LOCUS       DADXTH010000001        89637 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.001, whole genome shotgun sequence.
ACCESSION   DADXTH010000001 DADXTH010000000
VERSION     DADXTH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.001"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(45..1085)
                     /locus_tag="IWI97_000001"
     CDS             complement(45..1085)
                     /locus_tag="IWI97_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBC2461545.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1362..3164)
                     /locus_tag="IWI97_000002"
     CDS             complement(1362..3164)
                     /locus_tag="IWI97_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2461546.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3178..3930)
                     /locus_tag="IWI97_000003"
     CDS             complement(3178..3930)
                     /locus_tag="IWI97_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2461547.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3939..4301)
                     /locus_tag="IWI97_000004"
     CDS             complement(3939..4301)
                     /locus_tag="IWI97_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBC2461548.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4477..5349)
                     /locus_tag="IWI97_000005"
     CDS             complement(4477..5349)
                     /locus_tag="IWI97_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBC2461549.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5400..5888)
                     /locus_tag="IWI97_000006"
     CDS             complement(5400..5888)
                     /locus_tag="IWI97_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBC2461550.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(6026..6817)
                     /locus_tag="IWI97_000007"
     CDS             complement(6026..6817)
                     /locus_tag="IWI97_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBC2461551.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GEFYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(6872..7480)
                     /locus_tag="IWI97_000008"
     CDS             complement(6872..7480)
                     /locus_tag="IWI97_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBC2461552.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7473..7790)
                     /locus_tag="IWI97_000009"
     CDS             complement(7473..7790)
                     /locus_tag="IWI97_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBC2461553.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7946..8674)
                     /locus_tag="IWI97_000010"
     CDS             complement(7946..8674)
                     /locus_tag="IWI97_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBC2461554.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8833..9714
                     /locus_tag="IWI97_000011"
     CDS             8833..9714
                     /locus_tag="IWI97_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2461555.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9951..10994)
                     /locus_tag="IWI97_000012"
     CDS             complement(9951..10994)
                     /locus_tag="IWI97_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2461556.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10997..11665)
                     /locus_tag="IWI97_000013"
     CDS             complement(10997..11665)
                     /locus_tag="IWI97_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2461557.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11807..14143
                     /locus_tag="IWI97_000014"
     CDS             11807..14143
                     /locus_tag="IWI97_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2461558.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14258..14569)
                     /locus_tag="IWI97_000015"
     CDS             complement(14258..14569)
                     /locus_tag="IWI97_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461559.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14750..16225
                     /locus_tag="IWI97_000016"
     CDS             14750..16225
                     /locus_tag="IWI97_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBC2461560.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16272..16856
                     /locus_tag="IWI97_000017"
     CDS             16272..16856
                     /locus_tag="IWI97_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBC2461561.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16858..17277
                     /locus_tag="IWI97_000018"
     CDS             16858..17277
                     /locus_tag="IWI97_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBC2461562.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17558..17842)
                     /locus_tag="IWI97_000019"
     CDS             complement(17558..17842)
                     /locus_tag="IWI97_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBC2461563.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18194..18904)
                     /locus_tag="IWI97_000020"
     CDS             complement(18194..18904)
                     /locus_tag="IWI97_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBC2461564.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18901..20373)
                     /locus_tag="IWI97_000021"
     CDS             complement(18901..20373)
                     /locus_tag="IWI97_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBC2461565.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20511..20984
                     /locus_tag="IWI97_000022"
     CDS             20511..20984
                     /locus_tag="IWI97_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2461566.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21137..21688)
                     /locus_tag="IWI97_000023"
     CDS             complement(21137..21688)
                     /locus_tag="IWI97_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBC2461567.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21793..22323
                     /locus_tag="IWI97_000024"
     CDS             21793..22323
                     /locus_tag="IWI97_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBC2461568.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22806..23249)
                     /locus_tag="IWI97_000025"
     CDS             complement(22806..23249)
                     /locus_tag="IWI97_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2461569.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23265..24425)
                     /locus_tag="IWI97_000026"
     CDS             complement(23265..24425)
                     /locus_tag="IWI97_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2461570.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24932..26632
                     /locus_tag="IWI97_000027"
     CDS             24932..26632
                     /locus_tag="IWI97_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461571.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26734..27567)
                     /locus_tag="IWI97_000028"
     CDS             complement(26734..27567)
                     /locus_tag="IWI97_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBC2461572.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27871..30036)
                     /locus_tag="IWI97_000029"
     CDS             complement(27871..30036)
                     /locus_tag="IWI97_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2461573.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30296..32590
                     /locus_tag="IWI97_000030"
     CDS             30296..32590
                     /locus_tag="IWI97_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBC2461574.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32583..33248
                     /gene="pgmB"
                     /locus_tag="IWI97_000031"
     CDS             32583..33248
                     /gene="pgmB"
                     /locus_tag="IWI97_000031"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBC2461575.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33241..34248
                     /locus_tag="IWI97_000032"
     CDS             33241..34248
                     /locus_tag="IWI97_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBC2461576.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34270..35289
                     /locus_tag="IWI97_000033"
     CDS             34270..35289
                     /locus_tag="IWI97_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2461577.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35570..36937)
                     /gene="nhaC"
                     /locus_tag="IWI97_000034"
     CDS             complement(35570..36937)
                     /gene="nhaC"
                     /locus_tag="IWI97_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBC2461578.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(37046..38467)
                     /locus_tag="IWI97_000035"
     CDS             complement(37046..38467)
                     /locus_tag="IWI97_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2461579.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38710..40587)
                     /gene="tdc"
                     /locus_tag="IWI97_000036"
     CDS             complement(38710..40587)
                     /gene="tdc"
                     /locus_tag="IWI97_000036"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBC2461580.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40878..42134)
                     /locus_tag="IWI97_000037"
     CDS             complement(40878..42134)
                     /locus_tag="IWI97_000037"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBC2461581.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(42246..42451)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42714..44015
                     /locus_tag="IWI97_000038"
     CDS             42714..44015
                     /locus_tag="IWI97_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBC2461582.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(44084..44683)
                     /locus_tag="IWI97_000039"
     CDS             complement(44084..44683)
                     /locus_tag="IWI97_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2461583.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            44937..45818
                     /locus_tag="IWI97_000040"
     CDS             44937..45818
                     /locus_tag="IWI97_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2461584.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            45831..47441
                     /locus_tag="IWI97_000041"
     CDS             45831..47441
                     /locus_tag="IWI97_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBC2461585.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(47518..48177)
                     /locus_tag="IWI97_000042"
     CDS             complement(47518..48177)
                     /locus_tag="IWI97_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBC2461586.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(48460..49125)
                     /locus_tag="IWI97_000043"
     CDS             complement(48460..49125)
                     /locus_tag="IWI97_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBC2461587.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(49272..49877)
                     /gene="dhaL"
                     /locus_tag="IWI97_000044"
     CDS             complement(49272..49877)
                     /gene="dhaL"
                     /locus_tag="IWI97_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBC2461588.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(49905..50915)
                     /gene="dhaK"
                     /locus_tag="IWI97_000045"
     CDS             complement(49905..50915)
                     /gene="dhaK"
                     /locus_tag="IWI97_000045"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBC2461589.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(50890..51336)
                     /gene="dhaM"
                     /locus_tag="IWI97_000046"
     CDS             complement(50890..51336)
                     /gene="dhaM"
                     /locus_tag="IWI97_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBC2461590.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            51439..51759
                     /locus_tag="IWI97_000047"
     CDS             51439..51759
                     /locus_tag="IWI97_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBC2461591.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(51871..52740)
                     /locus_tag="IWI97_000048"
     CDS             complement(51871..52740)
                     /locus_tag="IWI97_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2461592.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(52836..54140)
                     /locus_tag="IWI97_000049"
     CDS             complement(52836..54140)
                     /locus_tag="IWI97_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2461593.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(54137..54646)
                     /locus_tag="IWI97_000050"
     CDS             complement(54137..54646)
                     /locus_tag="IWI97_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461594.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(54821..56956)
                     /locus_tag="IWI97_000051"
     CDS             complement(54821..56956)
                     /locus_tag="IWI97_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2461595.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(57039..58694)
                     /locus_tag="IWI97_000052"
     CDS             complement(57039..58694)
                     /locus_tag="IWI97_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBC2461596.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(58696..59010)
                     /locus_tag="IWI97_000053"
     CDS             complement(58696..59010)
                     /locus_tag="IWI97_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBC2461597.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            59180..59440
                     /locus_tag="IWI97_000054"
     CDS             59180..59440
                     /locus_tag="IWI97_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBC2461598.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            59454..59768
                     /locus_tag="IWI97_000055"
     CDS             59454..59768
                     /locus_tag="IWI97_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBC2461599.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(60498..61052)
                     /locus_tag="IWI97_000056"
     CDS             complement(60498..61052)
                     /locus_tag="IWI97_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBC2461600.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(61178..63107)
                     /locus_tag="IWI97_000057"
                     /pseudo
     CDS             complement(61178..63107)
                     /locus_tag="IWI97_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(63413..64129)
                     /locus_tag="IWI97_000058"
     CDS             complement(63413..64129)
                     /locus_tag="IWI97_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBC2461601.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(64130..65953)
                     /gene="glpO"
                     /locus_tag="IWI97_000059"
     CDS             complement(64130..65953)
                     /gene="glpO"
                     /locus_tag="IWI97_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBC2461602.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(65967..67463)
                     /gene="glpK"
                     /locus_tag="IWI97_000060"
     CDS             complement(65967..67463)
                     /gene="glpK"
                     /locus_tag="IWI97_000060"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBC2461603.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(67599..69059)
                     /locus_tag="IWI97_000061"
     CDS             complement(67599..69059)
                     /locus_tag="IWI97_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2461604.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(69049..70344)
                     /locus_tag="IWI97_000062"
     CDS             complement(69049..70344)
                     /locus_tag="IWI97_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBC2461605.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(70676..71353)
                     /locus_tag="IWI97_000063"
     CDS             complement(70676..71353)
                     /locus_tag="IWI97_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBC2461606.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(71383..72474)
                     /locus_tag="IWI97_000064"
     CDS             complement(71383..72474)
                     /locus_tag="IWI97_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBC2461607.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(72575..73009)
                     /locus_tag="IWI97_000065"
     CDS             complement(72575..73009)
                     /locus_tag="IWI97_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2461608.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(73593..75050)
                     /gene="ascB"
                     /locus_tag="IWI97_000066"
     CDS             complement(73593..75050)
                     /gene="ascB"
                     /locus_tag="IWI97_000066"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBC2461609.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(75047..76942)
                     /locus_tag="IWI97_000067"
     CDS             complement(75047..76942)
                     /locus_tag="IWI97_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2461610.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(77000..78775)
                     /locus_tag="IWI97_000068"
     CDS             complement(77000..78775)
                     /locus_tag="IWI97_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBC2461611.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLDESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(78825..80321)
                     /locus_tag="IWI97_000069"
     CDS             complement(78825..80321)
                     /locus_tag="IWI97_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2461612.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(80515..82137)
                     /locus_tag="IWI97_000070"
     CDS             complement(80515..82137)
                     /locus_tag="IWI97_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBC2461613.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(82465..83778)
                     /locus_tag="IWI97_000071"
     CDS             complement(82465..83778)
                     /locus_tag="IWI97_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBC2461614.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(84023..84247)
                     /locus_tag="IWI97_000072"
     CDS             complement(84023..84247)
                     /locus_tag="IWI97_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461615.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(84267..84689)
                     /locus_tag="IWI97_000073"
     CDS             complement(84267..84689)
                     /locus_tag="IWI97_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBC2461616.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(84833..85543)
                     /locus_tag="IWI97_000074"
     CDS             complement(84833..85543)
                     /locus_tag="IWI97_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461617.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(85561..86058)
                     /locus_tag="IWI97_000075"
     CDS             complement(85561..86058)
                     /locus_tag="IWI97_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2461618.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(86095..86547)
                     /locus_tag="IWI97_000076"
     CDS             complement(86095..86547)
                     /locus_tag="IWI97_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2461619.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(86682..87287)
                     /locus_tag="IWI97_000077"
     CDS             complement(86682..87287)
                     /locus_tag="IWI97_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBC2461620.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(87737..88357)
                     /locus_tag="IWI97_000078"
     CDS             complement(87737..88357)
                     /locus_tag="IWI97_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBC2461621.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(88359..89228)
                     /locus_tag="IWI97_000079"
     CDS             complement(88359..89228)
                     /locus_tag="IWI97_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBC2461622.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(89251..89613)
                     /locus_tag="IWI97_000080"
     CDS             complement(89251..89613)
                     /locus_tag="IWI97_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBC2461623.1"
                     /translation="MAKEEKALSPEATEKIQSEITQLKQEISTTQDKHKLAKLYEQVG
                     LKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRADAARAGDDQGIDYYMG
                     KMDEMRQIAKQVTIKGNK"
BASE COUNT        26168 a        18785 c        15574 g        29110 t
ORIGIN      
        1 taaaaagggg atgtgacact gtcacactcc ctttttaatt tattctattt ctttatattt
       61 attttgtatt ccgttacaaa ctcgcgtact tggttaggtt ccagagtgac atctccaaaa
      121 ctttcaagtc gctccgctcc tggcagttct tgagtctcaa atgtgatacc gccatgattt
      181 actaatttat tcccgcgcat ttccggtcca tcttctccaa aatttgctgt aaaaatcacg
      241 attcctgaag catctgtttt tacttggatc gatacttttt catctgggct tgtcaattcc
      301 gcagcattta gtaaaccaga ttgttttttc aacaaaaatg gatgatcgat gccgcccact
      361 atcattgatt ggcgaaaatc actggcaaag acctcaccta attttttagg acgttggaag
      421 tcaaatgctg tgccacttac tgaaactact tctcctgtag gcgttgtatc tttattgagc
      481 gtagcaaata atcggctatt caaccaaagt atatgttcgt cgattgactg tgctggatca
      541 ccggttaaat tgaaatagac atgatttgta ggattgaata ctgttttgcg gtcactgatc
      601 ccttggaccg tcactcgcca tgtatcatcg ttcgtcagcg tataccgtac ttctacatct
      661 aaagttccag gaaatccgtt ttccatatct gggctagtct gatagaaaat gaccgatgct
      721 tcgttttcac cattgatgac tgtataatgc cactgcttgg cttcaaagct aggtgcacca
      781 ccatgtaaac aatgaccagt gtccgggtct gtttttactt cgaacgtttc atcatctaat
      841 ttgaatgtgc cattcgtaat ccgtccagcc atacgtccga ttgaagcacc gatataaggg
      901 tctttttcta gatattcttt tgcttcatca aaacctaaga tcaactctcg tttttcatta
      961 tcgattattg tttgaaaatg aacaacccgt gccccaagat cactgacaga caaagatgtc
     1021 ccattttgat tcgtgatcgt aatcaagtga tagttttcgc caaaattttg ttcattgatt
     1081 tccatgttcc ttgcaccctc ttttctttca tttgcactga ttccagcata catggaaacc
     1141 gttttcttaa cagcttattc cgtgagtaac gagaaaaaat ttactatatt ctaataaaag
     1201 accgtgaaaa aaaggttgtg acaaaaatat tgtcacaacc tctttttccg aacaaacggt
     1261 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg aggagctatt
     1321 tttgcttggc tgttcttatt acttgatgca ggacagcttt ttcacaatct ctttttcaat
     1381 acgattccag tatataacca tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat
     1441 atcgtaaacg gaacccaaat atctttgtat ggattttgca tgaatagacc aaacatttgt
     1501 aatccgaata agacatggac tacacctaag attcctggtg ctaagaataa cacgcctact
     1561 tctctacgga tcgattgttt aagcaacccg cgtcgtgttc ctatcttttc aagcatttca
     1621 taacgagcaa tgtcactctt gcttcctgac aagatcttga acatcaaaca gctagcaagc
     1681 atggtcaaga aagctaaacc taggaagaat cccataaatt caaaaccaga gaatagacca
     1741 ttatacactt catacatttc atatttctgg ttgctgtact ctactttttg caaagatgga
     1801 ttgttcattt tattttgagc aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct
     1861 tttacctgaa ctagctgtaa agtggattgt ggcaagttca gttgatcaaa ttctgattga
     1921 ctgactacct tgatttcttt ttcttgctgt tccggcaatt catagcttcg aagttcctct
     1981 tgtacagtca gttcattcat ttttttagca ggaacttttt ctttcgtgat cttcggcggt
     2041 gttccatctt catgttttat catgatcaat ggttgagccg caaattcact tgcattatag
     2101 taaatcgttc ccgcatcttc tttttgagta taagaagcag aaagtgttgg tgaaagttct
     2161 ttgatttttt gctgatcttc tgcccgagga ttattcatca ctagatcata tccagaattg
     2221 ttttctgtca agtctgcaat ttcattttta aaacctaatc ctaccgtcaa agcgcccagt
     2281 gccaaggcaa acagcatagc gaccattgag agcatttgtg tatattcacg aatacgaaag
     2341 tttaactgtg acaatgtgaa attgttcaat tttttcaatg aaatctgttc tgatttcttt
     2401 agtaatgata aaaagaaaat gatcacagaa tggaaaatga aataagttcc taatacgatc
     2461 gtaactaagg ctacccctat accaaagact tgataggtcc ccacgtgttt catcatatag
     2521 taaccgcagc ctagaaaagc aattcccagt actacttcaa ggaaaaacaa gaatttatgc
     2581 tgcttgattc ttgttggtgt ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca
     2641 atcgaaaaag cattgacaac agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg
     2701 atcaacaacc cgtgaatact aaaaggtgta aaatgcgaaa tattgagatc taactgatcc
     2761 actaataatc ggtggacgat acttgtcaat cctacaccga cagctgaacc cactaaagta
     2821 gctgcgatcc ctactaagaa tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt
     2881 gctcccagca tcataaacat tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt
     2941 gcatataaaa tataaacgaa tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg
     3001 ataactgtag ccgcaacgat cgtattactt ttcaaaaaag cttcgttgct tgccattgat
     3061 tcaaacatgt aaaaaatagc agatgccata actaatcctg agaataacac caagtagtct
     3121 cttaaacggc tcttgatccc agtaagtgat aatttccaaa gcatagtcaa tctcccccta
     3181 ctgttccaag tttccaagaa tagatagtat ttctcgatag aaggaatcac ggctttgatc
     3241 tccacgtttc acttcttgat ggataccgcc atccttgatg aataaaatac gttggcaata
     3301 acttgcagaa aacggatcat gcgtcacaag aagaatcgat acatgatctt ttgtattcaa
     3361 ttcgtccatc gtatctaaaa gatcccgggc acttttggaa tccagcgcac ctgtaggctc
     3421 atcccctaat agaatcgttg gttgtgtgat caatgcacgt gcggcggcga cccgttgttt
     3481 ttgtccaccg gaaatctcag aaggatagct ttccaaaata tgagagatgg ataaacgttc
     3541 tgcaattttt tggacacgtt gcttgatttc ttttggtctg acgccttgta aagaaagagg
     3601 aacagcgata ttttcttgtg ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa
     3661 gccaatctca ttcgcacgaa aatctgctag ttgatttcct tttaatgtgg tcacatcttt
     3721 ttgattgatt cggatatgcc catctgttgg tttatctaat gtggataaaa tattcaaaag
     3781 tgtcgactta ccagaaccag aagcgcccat gatcccaata aattcgcctt tttctacatc
     3841 gaagctgatg ccgcttaatg cttgcgtctt tttctcattg ttttttccgt atacttttgt
     3901 tacattctgt acttcgacta ttttgttcat ttctttccct accctttaat tagtttttcc
     3961 agtgctgttt tcggcacgtc ttttttttcg acttcttcat acgaagtgac acctttattt
     4021 ttgttccagg tgatttttaa atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg
     4081 aagtccagtt ctttttcctt accctctttc gtatagccag gcaggatata tctatagttg
     4141 actgtttctt gtccagagtc tgattttgaa actgtttttg taccatctgt cgtaatttgt
     4201 acatagtatt cttctccacc cataactacg taatctgcga ttttcacccc tgcaagtacc
     4261 acaacgatga aaacaagtag accgatcaga agttttttca ctttttcccc ttctctctta
     4321 ctttatatac tgattataga aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct
     4381 tctttcaatt ttgtcatttt ctttaaaaca actaaaaaaa gagttcaaga caataatccg
     4441 tcctaaactc cttcttccta tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc
     4501 tgtattgata acaggttgcg ttcctttgtc tgtaatgatt gagacaagta aattattgat
     4561 caattggact ttccgatcat cggtgaaatt gatttcttgt ccatcttcga tttgctctaa
     4621 ggccatttga gtgattgtga cagctccttc aacaatggtt tgtcgagcag acaaaatggc
     4681 ttttgcttgt tgacgttgga gcatggcact agcgatttct gttgcatagg ctaaatggtt
     4741 caagcgtgtt tcgattactt cgacacctgc tactgctaaa cgctcttgca gctctttttg
     4801 caactcgtca gacactgccg tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt
     4861 atcatacggg tattggctag cgatatgccg aatggctgtc tcactttgga tttctacaaa
     4921 atcttgataa tacgctacat cgaataatgc ctttgctgta tcaacgactt taaagactac
     4981 tactgctgag atttcgatgg ggtttccatc caaatcattg acttttaaaa cagaactatt
     5041 gaaattccga actttcaacg aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa
     5101 gccattttct cgaatcgtac ctaaatattg tccaaaaaac aaaattactt ttgcttgatt
     5161 aggactgact acagttgccg aacttaacaa caataacgtg atcaaccaaa gaaaaatact
     5221 taaaaaaagt gcccaaactt tcgcttgcca catacctaaa tagaagaaaa acaatccaac
     5281 taaagctaat acgaccaatc cgacaatccc tgcataacca ttgatataaa atgctctttt
     5341 ttctctcatg ctacttcctg cgctccttta tccttcaata aatatctaat gttacttttt
     5401 taattttttt ttcgaatacg cgccattaca ccttctagtt ctaactcttt gtcttcagtg
     5461 atgtaccaac cgcttgcctg tgtccctccg gctgcctgcg gtaaataatc caattttgtt
     5521 cgaacccagt gcttttcttc tgtaaaatag tcttctacca agacctctag atccatccct
     5581 gctgaaagag gcaaatcttc ccgaacagat ttaaagataa atcgttcctt cttcccatca
     5641 tacttcaata ttcctgtatc gataatcggt aaagaataat aatccagctc tttcttcacc
     5701 gtcaataaat gtttgaaaac ttcatggtac acttgctcta attggcgacc ttctgaattg
     5761 ttcgggtaat taaccatcac tttttctttg tctggatcat agaaggtttg gcttagttga
     5821 gtgatataag tatgtaatct ttccatttct gtttttaact tttctctttg accttcttct
     5881 accttcatat actctttcac cctgttttct ttttagatat tttctttctt attttactat
     5941 agttatgtgc gttaagcgat gaaaaacagc aaagattgga aggatctttg ctgttttttc
     6001 ttaaaatatc cagttgagta caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa
     6061 tattgatcga tgtgtcttta gcagtgatct ttagcttttt ctgtccttgt tctaacaaag
     6121 tatttgttat tggctggtag tctttttcaa tataaaattc accagacgtt tggatagtat
     6181 agccactgtt ttccatctca tgcaaagata aattgccacc gtttaggatg acttttcctt
     6241 ctttgctgat caaactgtct tctagatgga agttgctgtt ttcacctgtg acgatcaagt
     6301 tatccacatt ggcctcatct aatcgaagtg agcctaattc atctttcgca gttattgttc
     6361 ctcctgtaaa ttcattgaac gatacccagc catcatgtgt cttagtattc acttttttga
     6421 ttgatacttc gttgatatca attgcactat tattcccttc gattgtcaat tcagataatt
     6481 catgatctgc agggacaaca atagtgattt gtgcacttgt gtcttctaaa ctaatgattg
     6541 cacctgttga ttttttgact gcattcacag tcaacacgcc atttttctcc tttatttctg
     6601 gcttttctgt cttatcataa gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa
     6661 ctgtttgatt ttctgcatgg atgacgattt tgtttgcatc cttgattttt tttgtctcag
     6721 taaccatatc gatcaaccga ggaccattat cccaagtcag actcttttga gcgcctaatg
     6781 tataagttgc acctgctagg aggccaccag taagcatcag accaataccg gtaatcaaaa
     6841 aatgtttcaa tctcaattta tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt
     6901 ttgtaataaa acgtcgtccc atccatttaa caaagttcat catgacataa aaaagccctt
     6961 ttgttccttt cagcaacagt ggcgaaataa atactgcgct tcctgtggcg aacagaccga
     7021 caccgataaa gaataatcca ctcagaatcg attgtccaaa cacactgatt cctgagatga
     7081 tcatgaatag gccaacacct aagatactga tacataaaac atagatacta aaaatgagcg
     7141 ctagtaaagc aaccaatagt cccccaataa ccaatataaa ccccagtgct agtggaatga
     7201 ggacaggaga agcaaataaa ccgataatga ttaaccaaat caggcgaatt tgtctttttg
     7261 gatttttcac taactcgttt tctgcttgtt cttcttctga aaagtaactg atttttaatt
     7321 ttaaggcaaa ctgctttggt gtaccatatt catcaatgat ctgatctaat gtttttccag
     7381 aatctaaaaa atattcctca taatactgga tcaaatcaaa acgatcgtct tcaggaattt
     7441 cttgaagatg atccacaact tcattcaaat attcattcat ttgtgctctc ctccaataac
     7501 cggtctaaag aatctcgaaa aacaatccat tcatcaataa tttcttctaa atgacgactt
     7561 cccattggtg tcaacgagta atatcgacgg tttctgcctt caaaaggttc atcataggtc
     7621 tcaagatatc cttctttttt caatcttctc aaaaccggat atagtgtaga ttcactgaca
     7681 gagagacttt cttgtacgat ttttgttaag gcatacccat acatatcttc cttgcttaat
     7741 gctgctagca cagtaccatc aagaagaaca gttggaatct ggatgtgcat tacgtcacct
     7801 tctattctca agtgtaatat tttatttcaa tctatattat acaacatata atatagtttc
     7861 aaaatcagtt tgtcaaatac aaaaaaactg actaaaagat ttgatccttt ttagtcagca
     7921 aaaaatggct tctctttgat ttttattata acagtggcat aatccaattt gtccacatcc
     7981 agctcagtgg catgataacg atcattgcag gtatcatgtc agctactggg aacattttca
     8041 cgttgatcat tctaaaaccg gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta
     8101 agatcatgtc tggtgtagtc aatggataaa taaattttgc caataagaac aaaatataaa
     8161 aaataatgaa ttggggaatt gctatcacag aaacgacata ccctaaatta cacgcaaaaa
     8221 tagctgctgt gaaaaaatca aggacagatt tagaaatcaa aatcgtactg tctcctgtca
     8281 tcccagaatc taaacttcca tagatccccg taccacttgc acaaaataga acaatgaccg
     8341 tcactaatgt gctgataaat tcatcgtggg tcataccaag cttttcacta gggaagattt
     8401 tagaaatagg tacttgcatc agcgcagccc ccttatttat cccattacct aaatgaacaa
     8461 tcaaaccaat cgctgttcct atcactaaag caaaaataac agccggcata tttttcatcg
     8521 gcgcaatgga atagattccc atccccatcg aacacacacc aaaaaccatc gtcagctgtg
     8581 ctttaaaatg ttcacttaat ttatttccta ctaatccgcc aattagtccg ccaaacaaaa
     8641 cggaacctac attgatcaaa actccagttg gcatcaaaaa actcctctct ttttctacga
     8701 agcgaaaaaa atcagaaaaa tttctgtaat tattgctttc gctgcaagaa agattgtaac
     8761 agacagagaa gattacacca attcattttt tgtgttaaag tattctttat tggaataagt
     8821 aggaggaata tgatggatat ccataaattc gaagtttttc ttgatttagc cgaaacaatg
     8881 aatttcacca aaactgctga tcgacaattc accacccaag gaaatatttc taaacagatt
     8941 ctttcgttag aaacagaatt agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa
     9001 ttgaccgaag ctggaagctt attgctccca tatgtaaaaa atgtcgtaga acaataccat
     9061 gctatgcaag aagtgcttga aacctatacg aaagacaaca atctttcatt aaatatctta
     9121 acgataccga caatgatcaa ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc
     9181 catcctgaat ttacagtcca gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac
     9241 gccaatgaac atagtgacac gatccacttt gctagaagtt tccaggcccc accaagttcg
     9301 attgagtggt tgcctactga aacagacgat tttgtagcag ttctacctaa aaatcatcct
     9361 ttagctagta aaaagaaatt ggatctaagc gaactgaaaa aggagaactt tttgctcctt
     9421 ggccccacta cgaacttgta tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa
     9481 ccaaaaatct cttataaagg agcaagaatc gacttgatca tcaatatgat tgcaaatgac
     9541 atggggattg ctattgttat ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg
     9601 attctcccta tttctccaac aacagagagt tatcttgcat ttactcgtaa agtcggtgag
     9661 cattctttgg cttcagatac attttgggag tatttaaatc aagaagaaaa ctaagcccta
     9721 cattttctgt ccatctatcc acccaaaaaa agagagcgac gcatgacttt cgctctcttt
     9781 cttgcatcat gtccttgttt caatgacacc tctaacattt ctcgtagttc aataagaaag
     9841 cagcgatttt tatgccaaaa gaaccgtaag aacatttccc taatcacaaa aaaacgacta
     9901 aaaaccccta aaaaccttca atagaatttt taggggtttt attctttttc ctattgttta
     9961 cttaaccgta tcattgctgt cgttcctact ccttcgatac tgctataatc aatacgtcca
    10021 tcatgcagtt ctagaatttt tttaaccagt gaaagtccta gcccattccc agaaatttga
    10081 cgacttgtat ctccttgata aaatttttca aacattcgat ctctggtctc ttcattcatt
    10141 cctacacctg aatcggtcac ttccagtaca atctctgtgg ctgtctcaaa tagtttgata
    10201 tgaatctgtc cattgatttg attgtacttt atggcgttat ccatgatatt cagccacact
    10261 tggtacaaaa actcttcatt gcctgtgtaa tttactgcag caagttcgat atccaattct
    10321 agctgttctt tctcccattt aggctgaaaa aaaacgatta cttctctgat ttgttcatct
    10381 aatcgatact ctttcttttc cagttggata cgctggtttt ccagtttgtt caattttaag
    10441 atgttctctg ttaattgtga aagctgcgtg atactttcga taatccgata taaaaatatt
    10501 tggcgttctt cgtcagagag atttggggct tgtaacaatt gaacatatcc ttggattgtt
    10561 gctagtggtg ttttgaattc atgggataca ctggacacaa agtcattcct cagtgtctcg
    10621 atactattga gttcttggac cataacgttg aaatctttat acagttgctg tacttctgct
    10681 actttctgct gttcatccat tcgaatcgaa aaatctcccg ttgctaccag actcatattt
    10741 gtccctagcg cactgattgg atgcaggata cgccgcccaa caaaaatcga tattcctgta
    10801 cccacgacca tactgaaccc cgcaaagacg aataacgggt ataacgttgg ttttccagcc
    10861 gattcagcag gcgagataga atgtcgagac aataagaaaa taaaaccgag aaaacacagc
    10921 agtgtcacta agatcgaaat gaagacaata ctagcaaaat agatccataa ttgtgaaaat
    10981 agcttttttt tcatctttaa ataaccgcct tatagcctag tccccgtact gtgatgattt
    11041 gaaaatcttc cgtcttttca aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc
    11101 gttcttctgt attcgtatct aatccccaaa tatcatccat caactgttgg cgagtgaaga
    11161 tcttgttggg ataagacagt aatttataca gaagcagaaa ttctttttga ggcagaatca
    11221 acgaatgctg atcttgtaac actgtataag catctagctc taattttgtt tgtccgatta
    11281 tcaagctact gctgtaattg atctttgaac gtcgaagcaa tgcttccaca cgtaggatca
    11341 tctcattgac atcgatgggt tttgtcatat aatcatctgc tcctaatttg aacccgcgtt
    11401 tcttatcctc aaaatcggcc tttgccgtaa tcattagaac aggtgtgtca aatctggtgc
    11461 tacgtaatga gtctagcaag tcatagccat ccattttcgg catcatgata tctgtaatga
    11521 ttaattctat acgtgtcgct tctagttttt tcagcgcatc ctctccattc aatgcggtga
    11581 tgactgaaaa gcccgctcgt tccaacacag aacggtaaag caactgtaat tgcggatcat
    11641 cttctactac taaaatacga ttcattgatc tccctctgtt ttttgaaaaa tttctagatt
    11701 ttttcttgta gttcatcttc agttaacatt tatttcgtat actacgatca ttgttctgat
    11761 atacttatta gtataccgaa tatttcaata cgggagaagg gtgattatgc ttcaattaaa
    11821 agatataaaa aaatattata aagtcggcga aaccaccacc aaagcattgg atggtgtgtc
    11881 tgtcgctttc agacaaaaag aattcgtagc gattttaggt cctagtggtt ctggtaaaac
    11941 gactatgctg aatgtgattg gtggactaga taattatgat tccggtgata tggtaatcaa
    12001 cggaaagtct actaaagact ttaaggacag tgactgggat gcctaccgaa acaactctat
    12061 cggtttcatt tttcaaagtt acaacttgat tggtcatcta ggcattattg aaaacgttga
    12121 acttgggatg acattaagtg gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc
    12181 attgcaccga gtcggcttga caaatcatat gcataaaaag ccaaatcagt tatctggtgg
    12241 acaaatgcag cgagtggcca tcgctcgagc actagccaat gacccagata tccttttgtg
    12301 cgatgaacca accggagcgt tagatacaga aacaagtatc cagatcatgg aattgatcca
    12361 agagttatcg aaagaaaaat tagtgatcat ggtcacacat aatcctgaac tggcccatca
    12421 atacgcagat cgaatcattg aattttctga tggtaagatc ttaacagatt cccatcctca
    12481 catcgaacga ccaaaagatg atcaatttaa cttacgcaga acaaagatga gtttttggac
    12541 agcgttaaag ctttccttca acaatatccg aacaaaaaaa ggacgtactt tcttgacctc
    12601 ctttgcttcc agtattggga tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt
    12661 ccaaaaacaa atcgataaca cgcaagctga aacgatggcg aaatttccga ttactatttc
    12721 taaggtaacg actaaccaga ctcgtgacga tgccggattg ggtgcaagca aagcggatta
    12781 tccagacagc aaaacgatta ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa
    12841 taagattgat caaacatacg tcgattacgt aacagacatc gatcctaact taagtaataa
    12901 tatcggattt acccgtacta caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca
    12961 gcctgtcagc ttctcgaatc aaaatcctga tacggaatct ctttctttat ctagtacgat
    13021 gtcagcaatg acaggtgtgg gagtgtcaag tttccctaca cagcttgaca ctagcaaaga
    13081 aaacttttta aaagacaatt attctttact tgccggaagt tatccagcct cagcaacaga
    13141 tgtcgtgctg atcgtagacg gcaataataa tactaatatc aacgcattga agaatcttgg
    13201 atttgatgtg aaagaagacg aaaaacttga ttttgatgat atcgttggta caacattcaa
    13261 attagtaaac aacaatactt actatacaaa acttccaact gggaatttta tccctaatac
    13321 agattatgat gcgatgtatc agaatgcttc tgatgaattg aagatctcgg ggatcctacg
    13381 cgtgaaatct tcttctacaa tgaatctctt atctccagga atcgcttata gcgatcaatt
    13441 aacgactcaa atcgtcaacg aaaacaaaga atcagaaatc gtcaaagctc aaagagacag
    13501 tggtgtaaat gtcttaacta ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag
    13561 ttatttaggt ggagacagct tgccttcaag tatcatgatt tatccgaaca attttgaaga
    13621 caaagaaaag atcttggatt atctagatga ttacaataaa ggcaaatcag atgaagataa
    13681 aatcatttat actgatttag ctggaacgat gactgaactg actggcggat tgatggatgc
    13741 tatcacttat gtccttattg cttttgcagg gatctcttta gtaacaagta tgatcatgat
    13801 cagtatcatt acttatacat ctgtgattga acggacaaaa gaaatcggtg tactaaaagc
    13861 tttaggtgca cgtaaaaaag acatcactcg cgtatttgac gcagaaacgt gtattttagg
    13921 tatttcttct gggatattag gtgtgtttat tgcatggcta gctacgttcc ctatcaatag
    13981 catcttatac aacatgacgg atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat
    14041 tattctagtg atcgtctcta ctatattgac gatgcttggc ggtcatcttc ctgcacgaat
    14101 ggcagcgaaa aaagacgccg ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat
    14161 ttagatttga gtcataagag cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat
    14221 atgttccttg tttcgcgctg ttttttttaa ttatttttta taattctggt atacttttct
    14281 ttcctttgta ccacaactct tctggcgtat acctgtcttt tccatttatg aatactgttc
    14341 catctgcttc aatcgttact tcagtcccaa tccacatatt acgagaaagt gcgagaagat
    14401 tttttctttt tcctcttcct ttttttccaa attgcaaggc atcggttatc tcttctgtcc
    14461 aatgagattt cacgtatttt acttctccgt tttcaaatgt aactttcaaa atattcgcta
    14521 tattttccac ttttactgct ttaggaaatg actcctccat ttttttcact ttccttcctc
    14581 caacactaag tatgcgcttt cttttagtga taagtatatc atagtttatt ctattatcaa
    14641 aaaaacgcat ccattccatt gtagaatact ttaccgaaca agttgaattg ttttcctctt
    14701 tttcaccttt tactatagat aggtaaagac aactaaggag gaatctctca tgacagatca
    14761 accgtatgat ttaagaaaag tcatcgaaga actaaaaaca cttccaggtc aatatcacga
    14821 aacagatatt gaaatcgatc ctgaagcaga attatctggt gtttaccgtt atatcggtgc
    14881 tggcggtaca gtaaaacgtc cgactcaaga aggaccaact atgactttca acaatatcaa
    14941 aggtttcccc ggcacacgtg tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga
    15001 aattttgcac catgattatc gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc
    15061 aatcaaacct gtgaaagtag acaaagcaga cgctccagca caagaagtcg ttcatctagc
    15121 taccgatgca gacttcgata ttcgcaacat tttgcctgca cctacaaata ctcctgaaga
    15181 tgctggtcca tacatcacaa tgggtgtagt cttaggatca gatccagaaa aaacaatgag
    15241 cgatgtaacg atccatcgta tggttctaga agataaagat acgatcggta tgtacatcat
    15301 gcctggcgga cgccacattg gtcacttcca aaaacaattc gaagccttga ataaaccaat
    15361 gcctatcact atcaatattg gtttagatcc agcaattgcc attggcacga ctttcgaacc
    15421 acctaccact ccacttggct acaatgaact atggtgtgca ggagctttgc gtaacgaacc
    15481 tattcaaatc gttgatggta tagcagtcga tgaagtgggt attgctcgtg ctgaattcat
    15541 cattgaagca gaaatcatgc caaatgaaac aatgcaagaa gatatcaaca cgaactcagg
    15601 aaaagcaatg ccagaatttc ctggctatac tggggatgct aatccagctg tacaagtagt
    15661 gaaagtcaaa gcagttactc accgaaaaga caatccaatc atgcaaacaa cgattggtcc
    15721 cagtgaagaa cacgtatcta tggccggtat tcctacagaa gcaagtattc ttgccttggt
    15781 cgataaagct attccaggaa aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa
    15841 attgatgacc atcatgcaaa tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca
    15901 agcagctatt cttgctttat cagccttcaa agaattgaag acagtcattt tagtcgatga
    15961 agatgttgat attttcgaca tgaacgatgt agtttggact ttcaacactc gtttccaagg
    16021 agataaagat atcatcgtga tcccaggaat gaggaaccat ccattggacc catcagaatt
    16081 accagaatat aatcctggct ctattcgttt ccgtgggata agtgcaaaaa ctatctttga
    16141 cggtacagtt cctttcgata tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga
    16201 agattgggaa aaatatttga aataatgaat gatctgcaac tgttttgaca tcataaaaag
    16261 gagtgcggaa aatggaacaa ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg
    16321 gcgcttcagg caccatttat gccatcaacc tagtgaagaa actaaaagaa tatccgatga
    16381 tagaaacaca cggaatcatc agtccatggg caaaacagaa cttaaagctg gaatccgact
    16441 tatcactaac tgaattcgaa agcctgtttg attaccatta ttcaaacaaa gatttaggtg
    16501 ctgctgtcgc cagcggatca tttttgaccg atggaatgat catcgtaccg gcaagtatga
    16561 aaacagtcgc aggtatctct gttggtttag gcgacaacct gattgcacgt gctgctgatg
    16621 taacaatgaa ggagcaacga aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca
    16681 ttcacttgga aaacttgact aaattagcac gactaggtgt gcaaatcata ccaccaattc
    16741 ctgcttttta taatcagcct caaacgatcc aagatcttgt taatcaccat acaatgaaac
    16801 ttttggatca atttgaaatt gtaaatgagc attcaaaacg ttggcagggt gattaatatg
    16861 gaaaaagaaa cgatagttga agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa
    16921 gtaatcggta aagacttatt gatcacttta acaggcggag atacaccaca tatcggaaca
    16981 gtcactacat tttctcgtga tacagagatg caaactgtac gtttccctag ccacgatggt
    17041 cggttccata aagacgatgt cttagccgaa aaaatagctg tgatcatcaa aactgccgtt
    17101 ccgggaagtt gtacgatcac ttccggtatc catgttgacc atatctccaa tgcacaaatc
    17161 aacgcctctt ttccgatgtc agaagagtta ggttacaaga ttttagattg gttggaaaaa
    17221 atagatttcc atatcaaaga accagtttat tataaagatg gtgaaaaacc attatgaaag
    17281 aagaggttgt gaaatcagcg ttatgacctg agtatgagag aaaaaaatgg aaaaatagct
    17341 tctcatattt tgccgttttt gagctaatac cgagggacag ctgattgaac gccgtttatc
    17401 aagaggttgt gataaagccg cttcgtttcg agtagtaaga aaaattttgt aaacatgggt
    17461 ttccacattt ttgcataaat ttgacttact accgagaaac tggcttttga acaccgttta
    17521 tcaagaggtt gtgacacgtc ttttgtcaca acctctttta tttttgcttc actgtaactg
    17581 ctacaatatc accgatttct tttccgatgt ttttacgaat atcttttcgt agtccaagga
    17641 tgtagcagac tgaaccatct ggatttttca cgcccatatt cacgatactg ccttcatatg
    17701 gcacattgtc aaaagttgct tggactttaa ctcttccagc accgaattct tttcttatat
    17761 catacggaaa aataatatat gccccgccct tgccgacttc ggaagcatag atttttgatt
    17821 caaaagaata aatttccctc attttatttc tcctattttt tacatctagt atagcatagc
    17881 aaaaaaaaga aggaactgtg acaaaagtct tgtcgcagtt ccttttttcg aataaacggt
    17941 gttctcctgc caactcctcg gtcacaagtc acaatagtca acaaacatga gaagctgttt
    18001 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc ttccttaaac
    18061 ggtgttcaaa agcttgcatc tacggcacta agcccaatca gatgaaaaat atggagtgct
    18121 atttttatct gattgttctt actgcttgat gcagagcagc tttttcacaa cctcctacat
    18181 ggtgttcaaa atattattcg ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt
    18241 ggaattcagc agctaaggct ttcgtacgtt gttcttgctc tgctttgctc atttttggat
    18301 tttcagtcat tgcttttacc ataccgtctt gaacataagc ttttgcttca ctttccatcg
    18361 tactttgctg tttcttcact aattcagcta acttggctgc ttggtccgta cttaattcta
    18421 cccatgtctc tgggattttg aatgtattca ctcggctgtc atattcgtga ttgttatcag
    18481 caatattgaa ccagaattta tacatgtcgt ttttgtattc ccaataagta gttttcgtca
    18541 ctttttcagc atcgccgtct gtcttttcta ctttatttgt ttcgttatcc gtaccagttg
    18601 cttttggttt ttcttgtttt tcatttgttc ggtaaagata cactttttct tttccattcc
    18661 ctaaagcttt atacaaaagc atatctgctc cttgggaatc agcactggaa acaatttttt
    18721 ctgtttttgt ttccgttact ttttccattc caaagtgatt ggacaaatta gcgacgatca
    18781 aacttagtga agcgacgaag attagtccga aaatcaaaga taaaaaggtt tgccatgttt
    18841 ttttagcgaa cacgttcgtt aaagcaaaag caagcaagct gacaatcaaa actccaataa
    18901 tcatgcatta tcccccgctt tcattagaac aggttctttt ttcttcaata aaaaggcaat
    18961 aataaagcca agaatcccaa agatgatcgc tacgataaac gcggcatggt aacctgacag
    19021 tgtggcattg attgcctgat ctttataagc taatggcgta ttatccaata cagatttgct
    19081 tgggatattt gcttttgtca cattcgtcaa cacactgatc aaaatagctg taccaatcga
    19141 gcttgcgatt tgacggaatg tattatttac cgctgtccca tgactgatca agttatttgg
    19201 caatgcattc ataccagaag tcgttaccgg catcatgacc atggagatcc caaacatacg
    19261 gatcgcataa aggatcacga tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg
    19321 tgcagttgcc gctgtcaaaa tgaacatccc tgtgatggct aaacgtttcg cgccgtattt
    19381 atcgaaaatc gcacccgtca caggcatcat agcccccatg atcaatgcac ccggcaacaa
    19441 catcaagcca gaatggaatg ctgattctcc acgaatattt tgaatataca atggtacgac
    19501 catttctgcc cctaccatcg ccatatttgt tacaccagct aaaacagctg caatcgtgaa
    19561 tgtagatgat ttgaatacac gaagttccaa gaatggatgt tccaaatgta attgacgcca
    19621 tgagaacaag gcgatgaaaa tcacaccaac aactaagaaa ccaattactt tcaagcttcc
    19681 ccagccgtca tctcctacac tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga
    19741 taaaatagcg gataaaatat caatgctagg atttgacaat ggaataacac ttttcattag
    19801 gaagaatgct aaaaccaaga caagaacaac gattggcagt accataccaa acaagtcacg
    19861 ccaatggtag ttatcaatga tataaccaga taatgtaggg cctaaagcag gagcaagtcc
    19921 aataacgatc cctgacatcc ccattgctga tcctcttttt tctggcggga aaatagatag
    19981 catgatattt tgcaataatg gcatcgatac tcccacaccg caagcttgga tcaaacgacc
    20041 gattagtaat gtactaaaat taggtgctgt aaagcaagta atcgttccga tcaaaaagat
    20101 cgtcatggca gaaagatata aatttcttga actgaattta ttgatcagcc atgctgttat
    20161 tgggatcatg atcccgttga ctagcaagaa accagtcgtt aaccattgca catcagacgc
    20221 tgaaatatcg aaagctttca tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa
    20281 aacggtacaa aatgtaccaa ttaataagac aacaactaaa agtgtccgat tatatggttt
    20341 tccgtaaata tctacgggtt ggttgttact catatttctc cttctttctg aattttttct
    20401 gaaactacta gaatacttta gtcctaaatt cgctatttgt caacctactt gacacataag
    20461 ttaatacgga tataattaag ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg
    20521 cttcccttaa acgtttgttg gaaaatgatc aattggttgt tggcatcagt gaattaagtg
    20581 atatcgtagg tgtgtctcca cgtcaattaa ggtattggga acaaaaggga ttcattcgtt
    20641 cgatcgaagc tgatgcgaat tgtcctagaa aatatcgttt acctacagtc ataaaagtag
    20701 aactgatcaa aaaatattta gatgacggat ttactttgtc aaaagctgca gaaaaggcag
    20761 aaatccgcca aaagaaaatg caccatgtcc gtaaagtttt ttcaaaagca atcaaagata
    20821 tcgaattgat cgacgaacgc tatacgattt tacaaatcgg taacttcaaa gaagagaatg
    20881 ctaagatgta catcattcat gacaacgaga cagacgaatt aacttacaag ctccttcctg
    20941 tcgatgaaaa agctgactac gaacagttgt taaaagaact ctaaacgcca ctaatctcct
    21001 aagtaccaag ctttttaata agtagatgct acataaatac ttgaaaaaag gcctgtttga
    21061 acataagaat atccgaacgg tgatctgcgt ttctttcata tagaaaggaa ttctccatca
    21121 ctgttcggat atttttttat ttttttcggt tactttcttc taatacatct gttagccaat
    21181 ttgcatattc tgtttcccga ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt
    21241 catgtttttc tttttcagta ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt
    21301 tttcttcatg aaggtcacgc tgctgctgaa taatttctac taatgccgga tcattcacgt
    21361 ctttgacgaa atacaatttc agaacaaatt cgtctcgatt aaccggtagt tcattggtgg
    21421 gtgtatgaat ccactctgcc aattgttcct tcccttcttc tgttaattca taaactttct
    21481 tttccaattt ttcacctgtg atctctactt gatgctgaat gattccttgc tcttccattt
    21541 ttgcaagttc aggataaatc tgactatgtt ttgcttgcca aaattctcct atttcatttt
    21601 tgaacgattg attcaattca tatcctgtca tcttcttctt acttaataac cctaacaaaa
    21661 tatatcgaag tgtatttttt cttgccatca gttttccctc cttgacaaat ctttaaatcc
    21721 attctatcat aaatgagtaa tcgaaaacat gtagtttatt acatgtttta atttataagg
    21781 agatgcttaa acatgaaaga atttaaagaa ttggaagatt ttattggaac tcactttatc
    21841 tatacgtatg acaatggttg ggaatatgaa tggtatgcca aaaatgacca tactgttgat
    21901 taccgtatcc atggcggaat ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc
    21961 gttaaattga cggacggcgt attcaaaatc acttggacag agccaacagg gacagacgtg
    22021 gcattggact ttatgccaaa tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg
    22081 gttgaagaac atccagaaat cactgttact ttccaaaacg aacatatcgc agaaatggaa
    22141 gcagctcgcg aaaaatatga aacttatcca aaattacttg ttcctgaatt tgctacgatc
    22201 acttatatgg gtgatgcagg tatagacaat gaagatgtta ttagcgaaga accatacgat
    22261 ggaatgccag atgatatccg tagcggaaaa tacttcgatg aaaattataa acgagtaaaa
    22321 taaggttgtg aaagagaggt tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa
    22381 cggaaaaata gttcctcatg ttttgccgtt ttttgagcta ataccgaggg tcagctgatt
    22441 gaacaccgtt tatcaagagg ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta
    22501 gttagaaaat agcttttcat attttctagg ctagtgggct tattaccgag aagcagccgt
    22561 ttgaacaccg tttatcaaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac
    22621 aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg
    22681 attgaacacc gtttatcaaa gattgtaaca actgcatatt cacacagtta aattagtgac
    22741 ttcaaaatga aataatgctc ccagcaaaaa attaggctgg gagcattatt tcgttattct
    22801 gttcatcaaa aatcagcgtt ttgattccat ttcatcacaa gttcctcctc atttactgct
    22861 tcatcttttt gctgtgtttg aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat
    22921 tgattttttt gatagactgt caaagtcagt gttttattgg tttcttttat ttttttcatt
    22981 agttgcgtac cgattctttg ttgccggtat tcccttttca caaagattcc tgcgataaac
    23041 tgatcaatca ccccagcaaa accaataatt tcttgatcta tttctgctaa gtaaatcact
    23101 gcatctggta aagctttcct aacagattct acttgattct cccaataagt tgatggaatg
    23161 aattcatgag aatccaagtt agcatgcagc caaatcgata gaacagattc cagttctttg
    23221 gcagacaatt ttgttgttgt gcggatcatt ttttctctcc ttacttattc attgatttta
    23281 aaataaaaac catttttttg aagctctttt acaagttttc gcccctcaaa aaagtatatc
    23341 ttctcttgtt ttacttttct aataccaaaa gaaacagcaa ctttaggatc atcagctgaa
    23401 acgattcgat aagttccacc aggcaattga tccagcttga ctttcccatg atggtcgctt
    23461 tgtactgaaa acggcacacc atttctagta agtgtttttt tcttatgttt caaaatgaat
    23521 gaactaccat ttaccggttt ccctgacgaa tcactaataa taaatgaaaa atcaagcact
    23581 tttttactta atctatactg atagattagt cgtccgataa acactaacag tatgactaca
    23641 gctattatcg ttcctgcaag aataaaccat tgccgcatgg ttctttcctg atttcctttt
    23701 ccttcttctt tttttaccga ttcgttataa ggaatccgat gtcctgtcac tagcaaacga
    23761 tgcgtgttga tcatatacgg cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa
    23821 atcgttagaa cagacgtgtc atctggcagt accacttgta tatttttaac ttgataagcc
    23881 aatttctccc ccaaaacagt caaaataaac gtgtctccgt tttctaaacg atctaattcg
    23941 gtaaataacc gtcgattagg taaaccgctg tgagcagaga tcactgaatg cgtcgaattt
    24001 ccaccaagcg ggaaagaggt tccttgcaag acgcctgctc cattttcaag gatcctacca
    24061 ttgagccgat cataaagtga aatatttaat tggatcttag gtatattgat acttccgatc
    24121 agccattcct ctaattggct ggatgttgta ctttcatttc tgttcttttc attgaaagga
    24181 tcagtaccag gattgaatcc ttggtcagtt aatcgttggt ttttctcctc cattttcttt
    24241 cgctgcactt caatttcagc tgcggtccgt ttttgtactt gttcgatacg tttttgatca
    24301 ataaaactat ttaaagaatc cacataaaat ggatataaag cgatcagtaa ccctacgacg
    24361 atacatatta ataaaaacaa acgaaacaaa agtcgacgag actgtttttg cttactctgc
    24421 ttcacatgtg tcctcccttg caagtttttt tgttctttct tttattatat gggttcagga
    24481 aaaaaataga aaatgaaagc cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa
    24541 gtacagccca aaaaaatagc tactcatatt ttttgttggg ttgggcttat taccgcagat
    24601 gcaagctttt gaacaccgtt taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa
    24661 taagagcgct agtcagaaaa tagcttctca tattttctag gctagtgagc ttattaccga
    24721 gaagcagctg attgaacacc gtttaccgag aggtcatgaa aaagctactt tgtttcgagt
    24781 attaagaaaa attctgtttc aaaacaagga gatgcaacaa aacttatgtc gcatcctctc
    24841 tccttagaac tttcacttat gtttttcttg taaaattcat aaatattttc atgaattttc
    24901 taatttcttg cctacctctc gcataggata tatgatgaat ttatctaaag tttgcaaagg
    24961 agttcgacaa ttgaaaaaat atggcccatt tttagcaatt tttttcattg ctttggcatt
    25021 tctcatgcct catttattca cacataaact aattttgggt gctgatgccc cattccatta
    25081 caatcggttc tatgatgccg cgcaacaaat taaatatggg gatttccatt attttccttc
    25141 cctttatgga ttccagcaaa gtggcaggat cgtgaatgcc gtttacggcc catttttcgc
    25201 ttatttacaa ggattgatcg tgttgcttgc aggtagctgg tttcattatc agattctttc
    25261 aaatttcatt ctttatctta tttcaggatt ttctatgctg attttactga gatatgtcaa
    25321 tgttcgaaat tgggtaagtg taccagtagc cgttttattc atgagtacgt attctattca
    25381 atactggtct atcaaccaag gattcactag ctgggggact tgtttttatc ctttatgtat
    25441 gatccctatt gctgatttcg tccttcataa aaagttccct gttatcaaag tcgctgtctc
    25501 agtgggactt atgacacaga tccatttact gtcttcgatc atgttagctt tgatgtatat
    25561 ccctttttat ctttattact tttttaacca gactgaaaaa ggaaaagcac tgcttcaact
    25621 gattaagtcg gtcttgcttt attttgttct gacagcaaat atttgggcag gattagctat
    25681 tgtttacagt gggaataaga ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc
    25741 gatagatctg gatggcagtt attggctcca ttaccctaag gcctttccta tattgatatt
    25801 atcaggtttt ttgcttttta ttttctttca acaaaagaac accttattcc aaaaaatcgt
    25861 gttgtacaca agtcttgttt tcttttttct ttctactagc ttgattccat ggacgacatt
    25921 gatcgaaaaa aatgtgcctt ttattagttt gatccagttt ccatttcgat ttttcgtgcc
    25981 atttacgcta ttgcttctac tttaccttgc cctttcactg aaccagtggt ctgaacaaaa
    26041 gtggttccgt cttttaagta tatttggcat tcttattttt tcagcacaga cgatccagaa
    26101 cttataccag catctggaaa agtgggaaaa tgaaacattt gtcagtcgac atacttatct
    26161 attcgatacg ccggaagaag caaggaaaac attcttcagc aaagatatgt ctaccagctt
    26221 gttcgttttc caaaaaacca cacctgacta tctgccaatc tataaggaga caaaagacaa
    26281 taaatacgac cggtataacg aagaaatact agaaaacgaa acaactttta taaaaacaca
    26341 tccaggcggt cttttgacaa tcaagtggaa aaacactggg gacaaaactg ttcatatccc
    26401 tgttatcgtc tacgatcgga ctgtgctaca gcaaaatgga aaaacattga ctgattatga
    26461 agtaacagat atcgggaccc caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct
    26521 tcattatcaa acgccaatct atttctattt cattctttct cttactttga ttggctggtt
    26581 taccttgcta tgtttattta tttatcatag atacaagctt ttacgtgctt aggcaaaagg
    26641 gataaataca tccgatcaaa aaactctcca aacggtataa tcgtttggag agttttattt
    26701 taatcagttt ttttatgtta tatgttatat tttttattcg ttcacatcta attcgatttc
    26761 caccccgtag tgatcactga tgattggact atcgatccca tcaaaaatcc gttgatattt
    26821 tttaacctgc attccttcag ggacgaaaat aaaatctata cgcaattttt ctgtattttg
    26881 ttcccaacca tctattttct tttccacagt agctgctttt cctttttcat tcgcgacaac
    26941 atacgaatct tgaagaagaa ggcaactttc tcttacagta taataacctt gagagtctac
    27001 tggattattg aaatcaccta ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc
    27061 caagtttttc cactcaaaat aaaagccact ttttttatct gtccaccaag aaaagtggca
    27121 gctcgagaca aaaatgtcct gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt
    27181 ctgataatct tctttctttt gaaattggga gacggtaagg acctgacttt ccaatgtttt
    27241 tttcgtaagc agtgcatttc cttcttcata tttatcataa ccgatgtggc tcatttccca
    27301 actccaaaaa tactcttttc cgtgttcctt taaaagctga actaaacggt aggcaaaatt
    27361 atctttacgg ataggtactt gattctttac cggacagaac ttcattagct tcgcaggttc
    27421 tacttcttca gattccagta attggttgat ttcttgtaaa gcaatcactt cataatcttc
    27481 taacaggatc ttatctgcta attcttgcaa ttttttctct ggttctggct caagccagct
    27541 atgcgtgttg agtgtcatga atttcattgg ttttctcctt gtcttcattt cgaaacaaaa
    27601 gggttgtgac aaaatttttg tcacaacctc tccagcaaat agtgttctcc tgctaactcc
    27661 tcagtaataa gctcaatgga ccaggagcta ttttttgttt ggctgttttt atcacttggt
    27721 gcagggcagc tttttcacga cctctccact aaacagtatt caaactgctg cttctcggta
    27781 ataagcccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt cttatgactt
    27841 gatgcaggac agctttttca caacctctcc ttacatttta atggagccga tcactttacc
    27901 agctgtcact gtacctaatt gcttgatcac tacttcttct actttatcgt ttgtaaatac
    27961 gatcatgatc gttggatctt tttcttcgtt ttggatcaca tcaagatcca tctcagctaa
    28021 tttcgttcct gttcctactt cttggccttc tttgacagca atctcaaaag ctggctgatt
    28081 catttggact gtatcgattc ccatatggat caaaacttcg ataccatctt tcgtttccag
    28141 cccaattgca tgtttgctag ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac
    28201 ttctcttgat gttggaataa cagcaaagcc gtcacccatc attttttgtg agaataccgg
    28261 atcttttact tcagataaag gaatcacttc tccagaagct gcggaaacta gttcttgttc
    28321 tatacctaag tattgcttac tacctgcttg tccatttcct gtagaaacca tttctggctc
    28381 tggaatctct gctcctgatt gcagcagatc ttcgatatct gatttcaaga catctgcttt
    28441 tggtccataa actgcttgga ctccattgtc tttgacgatc aatcccatcg cccctgctcg
    28501 tttccaggct tcttcagtcc ctactttttc tcgatcattt acacttacgc gtaaccgagt
    28561 catacaagca tcgacatctt tgatattttc ttttcctccc agcaaatgaa tgatctggac
    28621 cacttgctga tctgctgttc ctgttccgct gttagataca acggtttctt cactgtcatt
    28681 gtcgtaattt ccgttacgtc ccggtgtagc aaagttgaat tttttgatca agaaatttgc
    28741 taagaaatac gtaacaacac caaaagcgat cacacacaac acgaagttga taagatctcc
    28801 acctagaccc gctttgatag ctaacggtgt ccgagtcagc aattcgatat tcccaaatga
    28861 atggacccgc aatggcaaga tatctgccat cgcaaatgca gccccttgga tcactgaata
    28921 aatgacatat aatggcacag cagcaaacat aaacatgaac tccaatggtt ctgttacacc
    28981 tgttagaaag acagccaacg ctgctgaaaa atacattgat ttatatcttg ctttcttatc
    29041 aggatcgaca ttacgataca tcgcaaaagc cattcccatc aagatacctg aagaaccaat
    29101 catttgtcct actttgaaac gagccggcgt ccagttatgc aacacaaact catattgcga
    29161 catatcccct gcacctttaa gattcactaa atcagtggcc caagccaacc ataacggatc
    29221 ttgcccaaat acttgtgtcc ccgcctgtgc gcctgataaa atctcatacg atccgccaag
    29281 ctgtgtatag ttgataggaa tcgtcagcat atgatgcaat ccaaatggca atagtaaacg
    29341 ttctaatgtt ccaaataaaa acggtgctag aatcggtgcg ctttcttgtg attctgcaat
    29401 ccataaacca aaattattga ttccagcctg tacgtttggc cacaccacag caagaatcaa
    29461 tgccacgatt gttgaccata agataacaac aaacggaaca aagcgctttc cattgaaaaa
    29521 tgacaaagca tccggcaatt ttctataatt atagtactta ttataagcca ttgccccgac
    29581 aaatccagcg atgatcccta cgaatactcc catattcaaa gctggtgctt ccaatacact
    29641 tgtaaaaaat cctttaacca tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc
    29701 attcgcaagc atgtcacttg tgacaccaaa gatagcccca gtgatccgat tgattaaaat
    29761 aaatgagatc ccagctgcaa atgctccgcc tgctctttct tttgcccaac ttcctcctat
    29821 tgctaaagca aataaaatat gaagattacc aataattgcc caaccaatat tttcaattac
    29881 tccgcccgtt gtgacaagcg gcgtccaatc aggattgatc aagggaattg atttccctat
    29941 actgatcatc aacccagctg ccggcataac tgctaccaca accattaaag cctttccaaa
    30001 tttttgccaa aactcaaaac taaatatctt tttcattccg tttagctcct ttacgcaatc
    30061 ggtttcgtta aacgtattat agcgtgcgtt ttcttttttg taaatgcttt attttttatt
    30121 tttctgtcta taccattcta taaaacctat aatacgtgtg ttcccttcta taattttctc
    30181 ctaaaaattt atttttttaa gcttttttct atgaaatatt ttcttttgtc tattgacttg
    30241 tgcaatcggt tgcgtttatt atgaacgtat aaaacagctc attaggagga gaaaaatgaa
    30301 acagatcaaa cgactatttg ctattgatcc atggaaaatc agcacgacac aattagataa
    30361 aaaaaatctt cgactgcaag aatctttaac aagtattgga aacggttata tggggatgcg
    30421 cgggaacttt gaagaaactt actcagggga ccaccatcaa ggtacttatt tggcgggtgt
    30481 ctggtatccc gataagacac gtgtcggctg gtggaaaaat ggttatccag aatactttgg
    30541 aaaagtgatc aacgccttga actttatcgc gatagatatt tcgatcaacg atcagctgat
    30601 cgacttggct tctttggaac cagaagactt ttactgggaa ttagatatga aaaatggtgt
    30661 attatctcgt acattcacaa tcactacttc tactaataaa gtacgcattt ccttcgaacg
    30721 ttttctgagc attgtcaaaa aagaatcagc ttatatctat ttaactatag aaatgcttga
    30781 aggtagcgga acagtcaaac tcgtatcaaa attggatgga aatgttcaaa acgaagacag
    30841 caactatgat gaacattttt ggaatgagat tgatcgtgga actatagaag aaatcggcta
    30901 tttgacgact aaaactattc caaacaactt tgggattgaa caatttactg ttactgcagc
    30961 aatgcgccat ttcgttaatg gaaaatcagt catccctgac tatcacgaag atactttagc
    31021 attacatggt tgcttgtctg cagaattatc tcaaggtgag acatggtcag tggaaaaaga
    31081 aattatcgtt cttactagta gagatattcc agaagcgcag caagcagaaa aagctgtctc
    31141 agaattaacg aaccagacaa acaattatat ccaagcaaaa gaagagcaaa cagcggcttg
    31201 ggcagaacga tggaagcttg ccgatgtggt catcgaagga gacgaagaag cacaacaagg
    31261 tattcgattc aatttgttcc aactattctc tacttactac ggcgaagatg aacgattgaa
    31321 tatcggcccc aaaggattca caggagaaaa atacggcgga gccacttact gggatacaga
    31381 agcgtatgct gtccctcttt atcttgcttt agccaaacct gaagtaacta agaatctgct
    31441 gaaatatcgg cataatcaac tgccgcaagc aatccacaac gctcagcaac aaggattaaa
    31501 aggcgcgctt tatcctatgg tcaccttcac tggtgtcgaa tgccataacg agtgggaaat
    31561 cacattcgag gagattcacc gaaatggcgc gattgcctat gcaatctaca attatacgaa
    31621 ttacaccggc gatacggaat atttgaaaaa tgaaggactg gaagttttag cagaaatcgc
    31681 acgcttctgg gcagaccgtg tccatttttc aaaacgagca aataaataca tgatccacgg
    31741 tgtgaccggc ccaaacgaat atgagaacaa tatcaataac aattggtata caaatacgat
    31801 cgcagcttgg gtcttgcgtt acacgcgaga aaattattta acttatcaaa aagaagctgc
    31861 tatcactatc agcgaagaag aactagccaa atggaaagat atcgcagaga acatgtactt
    31921 cccagttgac gaagaattag gaatctttgt ccagcatgac acattcttag ataaagatct
    31981 catgcctgta tccgatattc ccacaagcga tcttcctctg aatcagcatt ggtcttggga
    32041 caaaatcctt agatcttgct tcatcaagca ggcagatgta ctacagggga tttatttctt
    32101 caatgatact tttacaaaag aagaaaagca aaaaaacttt gatttctatg aaccaatgac
    32161 tgtccatgag tcttcccttt cgccaagtat ccattcgatt ttagcagcag aattaggaat
    32221 ggaaagcaaa gctgtagaaa tgtaccagcg aacagctcgc ttggatttgg ataattacaa
    32281 taacgacaca gaagacggct tgcacatcac ttccatgact ggtagctggt tgtcgattgt
    32341 ccaaggattt gctcaaatga aaactgccca tgatcagttg agattcgcac catttctgcc
    32401 aaaaacttgg aaagcttatt ctttccatat caattatcgc ggacgtttat tgttcatctc
    32461 agtcacagat gaacaagtta ctttcgagtt attagaaggt tctcctctag aaatttacgt
    32521 ctatgaacag tcttattctt tagcggatcg tctagaaata acgctaagaa aggaggaagc
    32581 aaatgtttaa aggggtattg tttgatttag acggtgtcat taccgatact gctgaattcc
    32641 attatcgtgc ttggcgaaag cttggtcagg aaatcggcat tacgatcgac cacacattca
    32701 acgaacagct aaaaggcgtc agccgagaag actcactctc tttgctttta gcttatggcg
    32761 gaaaagaaca ctctttttca aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt
    32821 attatttaga gatgatccag acgatcgaac caaaagatgt cttccccggg gtcgtgcctt
    32881 tactggattc tttaaaagaa gaaaaaatca agattgctct tgcttctgca agtaaaaacg
    32941 ggccattcct acttgaaaaa atgggtctta caccttactt tgatgctatt gccgacccgg
    33001 cagaagcaac aaacggaaaa cctgcgccag atatcttcct ccttgcagca aaagctgtcg
    33061 gactaaaagc agaagaatgt atcgggatcg aagacgcgca agccggtatc caagccatcc
    33121 tctcaagtgg cgctcaacca attggcgtgg gacgtgctga agaccttgga gaggagatcc
    33181 cgcttgtccc tgatacgact tttcttacga tcgattactt gaaagaaaag tggcatgacc
    33241 atggataatc cagagatctc cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca
    33301 ttgaagaacg actacttaga agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc
    33361 ttcgcaccag acaaagaagg aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa
    33421 gatgttctaa cagacaaaag cttttttgga gcaactgtcg gacctgtagc cggaaggatc
    33481 cgggatgctt cttggggtga gcatagattg gagaaaaact gcggatcaca ccatatccac
    33541 ggtggcacga aaggctggtc tttccaattt tgggaagtag aagttttcaa aactccatca
    33601 tcaataggtg tggtctttta cttaaaagat gaattttcta cctatccagg accaatcact
    33661 gctacgatca cttaccgttt aacaaaaaac gaattagaga tgacaacctc tgctagtagc
    33721 cttgttgaaa ccatctgcaa tccgaccaac catgcctact tcaacctttc aggaaatgga
    33781 acaagagaca tctatgaaca tcagttaacg gtctttttag atggtgtgct tgaattagat
    33841 caagaaaaac tgcctactgg aaattggaca aaaaaagaaa aactgccaat cgactttaga
    33901 aaatccccaa cactgcaaga gatccttgca tgttatccag atggactaga tgatgtattt
    33961 cttttgcatc atcctaggct ttccaaaact tccttacaat tattcgagaa gcattctggt
    34021 cgccaaatga ctatagcaac ttctaataaa agtatggtat tattttctac gactggattc
    34081 gaagctgatt tctctgtcaa tggaaaacag atgcattcaa actacggatt agcaatcgaa
    34141 ccgcaagaaa tccctgatat cgttcatttc cccaaatttg gatcgatcaa cctccatcct
    34201 ggtcaagaac ggatcagtca gacgatttat cggttctctg cacaataaca agagaaaggt
    34261 gtttttatca tggctattac cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg
    34321 accgtctctc gtgtcatcaa cgatcatcct agtatttccg agacaacaaa gaagaaagtc
    34381 cgaaaaatta tggatgaact aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa
    34441 cgcatttcca gtgccatcgg cgtaatctta ccccctcttg attcaaaaga gcggatcggt
    34501 aacccatttt atcttgagat catggaagca atcaacgaag aagcgcggaa ttatgatatg
    34561 actacagcaa tcgctactgc taaaaatttt gaattgctgt tagagaacgt ccagcgtatg
    34621 catttacaaa aacaagtaga tggttttatt ctagtgtatt cagatagtca agatactgtc
    34681 attgattatt tataccaaaa ccacatccct ttcactttga tcggacagcc ttatcatcat
    34741 gagaacgaaa tcgtttatgt agataatgat aaccagcttc taggtaaaca agcaacagaa
    34801 tttttgatcc agaatggaca taagaatatc ctgtttgtga caaacacgac tcatgaaagt
    34861 ttgtatttcg aacgatattt cggttatcaa aaagcaatga tgatggctgg tcttcctgcg
    34921 ttatccagtg tagtactaga gcatccggag gattatctgt cctttgagaa aacgttgaaa
    34981 gaaaacgaag ccacagcatt ggtggtcatt gacgatatct tcgcccttcg cacgatgcag
    35041 ttggcgcaaa tgtatggcta tgtcattcca gagacactct ccgtcattag cttcaacaac
    35101 tcgatcttct caacattaac acacccttac cttacaagta tcgatatcga tatctccgaa
    35161 ttaggacgtg tcgctatgca aaagctgcgc gaattgatcc atgaagaatt gagcagtggc
    35221 gtacgtttgg tcatccctca tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac
    35281 ccatactaaa tagctgactc actaaatacg ctgaccattt atcctatggt cagcttttta
    35341 tgtaaaagaa gatactactt ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa
    35401 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac
    35461 tccttcggaa ataagctgaa attcacaaaa atttaaaaag caattttctt gagtttcttc
    35521 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttccttcctt caatttttta
    35581 cacgtcgacg attcaataaa aaaccgccta atattgtaaa gacaggtgca agaatcgaga
    35641 aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat cgttcccata atgaatgtgc
    35701 cgctgactcc ccacggtacg agagaattga cggtcgcacc tgaatccgct agtgttcgtg
    35761 tcaaataggt ttttgataag ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa
    35821 gaatgatgga cagatactgt tctccaacga tcagattgat tccgatacag ctcaaggcag
    35881 taaacccgac taaacgagaa ggtcggtcca tcttttcttt taattcttga acgatggtat
    35941 caacgatcag atatttgatc agtaacccac ctaatgccaa tgcaagtaaa atcaaactag
    36001 cagaacctaa catactcatg atcccgcctc gagaaagcag ttcatctagc tttggatcag
    36061 gtgtatttgc gacataacca ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt
    36121 tctcatggat aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat gtagggatag
    36181 ccggaatttt tttgattgcc cccccaaata aaataactag tggaatcaat gtccaaggag
    36241 agatccaaaa gctcgctttc atgttttgga tcatttcatt gatactagct agatcggctg
    36301 aatgactgtt atgtcctaat atcgtataaa aaatcaacga aaggacgaat gctgggaaat
    36361 ccgtataacg cataccagcg atatgttcga atagattaac tcctccaata cccgctgcca
    36421 agttggtcgt atctgataaa ggcgagcagt tattccctaa aaaggcaccg gaaacgactg
    36481 cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc
    36541 caatcgtcga aatcgttgta aatgaactgc cgacagtcac tccgacaatt gcacaaacaa
    36601 gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt aaatccataa accataatcg
    36661 tagggatggt tccagataaa atccatgaag caactaagac accaatcaaa agaaaaatca
    36721 ggattggaat gatccctgga gtgattcctt tgatgatccc gtcatggatc tcatcccatg
    36781 tgaacttttt aaatctgcca taaaacaaca aacacatgaa agctgcaagg atcgggatat
    36841 gtggcgagag ctggaatccg atgatcagta ctcctaggag tgccaacaac acgatcaata
    36901 cagcgatact ttcttttaaa gaaatatttt tattcatcgt cgatctccta tttcaaatga
    36961 aaaggccctg ccatgaaaaa actattcact tggcaggacc tcctactaaa tcagcgacaa
    37021 aacgtcggct gaaagactta tttttttatt tttcttctaa acttttttct acctcaatcc
    37081 cccaagaatc tttcttgaag tgattgatga tcaatggaat aacgaacatg accactgtga
    37141 tagctgctac taataatgca tcactcaatg tcgctgtacc taatgtagca aatacgacaa
    37201 cgatgataga gaagatcagc atgcagctga ctacccaagc taatccgttt ccgtttttac
    37261 caattcggta aggacgttcg aattctggat gtttcttacg cattttaagc aatgaaacag
    37321 caatcaaaat gtacacgata caataaataa cagttgtagc atttgtaagt gtcaagaata
    37381 cagcacttac gtcatccatt acaccataaa gcaaagcaaa taatgaaata acgattgatt
    37441 gtgttagaac gacatttcta gatacgccgt atttgttttc tttatggaat ccgaattttg
    37501 gtggaaggaa tccttcacga gctacttgga tgattgtttt tgatggtcca gtaacccaag
    37561 ctgataattg taaaagcaca ccaataaata ccatgaagct gaagatattt ccgatcactt
    37621 ctggtaagcc taggatttga cagtcaatca agataggctg cgtgatattt gctaattcca
    37681 tttttccatc tggtacgaca tttgctacaa gcatcgcatt gatgacattc aataaaacta
    37741 atccgatcaa agcgataaat acgccttttg tataattttt tgttgcgtct ttcaaacgag
    37801 gcatgtaaac agaactcatt tcgatcccaa caaaaataaa tgtgatacca gctaagtatt
    37861 tcaatgaatc aatatgttcc aagtcaggga aagcttttga ccatgaaaag tcacctagat
    37921 agccgtttga gactagtccg actttgaagg ctgccaaaac acctaaaacg aacatgatga
    37981 ctactgggat atatacccct agccatacac cgatgttccc gccgactttt gccatatcga
    38041 atttcaagtt caagatcgtg atgatccagt agataactaa aatacaacct aaaacgaacc
    38101 agtgattatt gccaagttct acattaccaa atgtgtttcc taacaatggt cctaaagtag
    38161 aagcgaccat taccatacct gggaacattt gcacccataa tagccatgca acgacaaatc
    38221 cccatttgct gcccagtgct gtttttaccc ataactgagg accgccttct tgaggaagca
    38281 tcgtggaaag ctcaccggaa accattgata tcggaccggc aaaaaacaaa actgcaaaaa
    38341 ttgaataaaa aatcaatgtc caaccgactg cggctaacgt aggaatactc cgaaccgttg
    38401 cacacagtgc cattgtcaga ccgataaagg tcccaagacc cattttactt acttcagatt
    38461 tactcaccgt tgacactctc cttgattttt tggataaaat aacggtgtct ttgaagtagt
    38521 ttcagcaata aacgaaacaa ttcagcgaat atttgagggg attctttgag tttattgctt
    38581 gaagggtttc ttcaaagaca acctcaaaac aaagggatcg aagagcttag gtctcccctt
    38641 gctcttcaga tccaaacatg cgtcagaaac agttagtatt caagctgttt tctaacatga
    38701 ttaccttgct tattttgctt cgcttgccaa taattggtct gcgtagattt tttccaattt
    38761 ttcttgaaga gctgctttga ttttttcagc atattcttca aagtgttctt ctttgttcat
    38821 gtatggtgtc attacgctgg cacgcaatac agttactttt ccggcacggt tccattcttc
    38881 atctgagaat cctagttggt tcacaaattg taatgggctg ttgccatagt ctgggatagc
    38941 aaagtcagta tgtgaagtta agaactcatt accgtaaatg cttcctttca cgtatgaaga
    39001 ataatcatat acgtcatggt tcaatttatt cattgcaact aagtcatcgt tgcctttttc
    39061 tttgaataca tagtctacca tattgaaatc tggataagtt aatggatgaa cttcgatttc
    39121 tttgtctcct actttaaatg acaagtcatt caagaagttg tagaaacgat gtgcaccttc
    39181 gattgatgca cccattaatt taccatatcc tgtgacattc aaaggtaaga catggtgtgc
    39241 tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct tccaagatat aagccccaag
    39301 taatgctggg atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag agataacgtc
    39361 tctcatgcgg atatctttga tgacgatacc accggcagaa tatggaacat agcccatttt
    39421 atgtgggtca atcgttacag attctgcttc ttcgattgct ttatatgcac tgtgtacttc
    39481 ttctaagata taatctttgt tttctgtaaa gacattgtat ttgtaatgaa catcttttaa
    39541 atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc
    39601 ataagcagca tctacgtgta agtagaagta aataccgtct ttttctaata cgcgacgcaa
    39661 tgcaacgatt ttatcgatac cgtcgattgc tccttcttct gttgatccta caacaccgac
    39721 tacacctagg attggtgttt tttcagctgc tagaccacga acgatttttt caagttcgtt
    39781 gatgtccatg cgatagttgt ggtcaactgg cactggaatc acttggtcta aaccgatacc
    39841 gatgatgtcg gcagctttta gccatgaata gtgtttagtt tgtggaacca accattttcc
    39901 taatttttct aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt cgattttttc
    39961 tgggacgctg tctaacagat tcatgatttc ttctgttgac aagttcatta attcccaatc
    40021 gctttttcca gcaactagtt caggtgtcac ttctttcatt gcaagcggca atgatttgat
    40081 gttgcgggca taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc
    40141 ccagccatct ttatagctca ttaattttgc aaattccatt cctacttctt cttccatttg
    40201 gcttgttgca ggagaagatt cataggcaac gttattacca ttccatagca tagcaaaatt
    40261 gtacgctagt aaagatggca tcaatgtttc agagttcata tgaccccagt aacgtccggc
    40321 attatgccaa ggaacggaat gggtacgcat ccgcgctgaa atttctgata gaacatcctt
    40381 tgttttattc acagtgtgtt caaaactagc actgcttttt tcttctggcg taataatagg
    40441 catgtcttga ggcatgtagt tttgacgcca gcctaaatgt tcatccacca attcattcaa
    40501 caaagcttta tagatttggc cgttctcagc tttgtcccca atgaataggg catttaggtt
    40561 tagatctttc gacaatgatt cactcatagt gaaaccctcc taaaaaaata tttaaatttt
    40621 gtcgagccct acattacacc tgttcttcta ttctgtaaac taagttttgg cctgattttt
    40681 aatcatattt taatatgtat caattatccg aattgttcac acttttgaaa taattaaaaa
    40741 aaacgaggga atttacaaat gtaaactccc tcgcaaagat tgtattaacg acaaccagcg
    40801 ttctatttta ggatgggttt caaaattgga atgttgtaat agtatttccc tttttcaagg
    40861 taaaaactta ctaaattcta atcaattact ttggccaaaa agtaattttt cttacctttt
    40921 cgaataacca caaacttacc atcgaattca tcggatggat ttatgacaaa atttaaatcg
    40981 gtaacgcggt caccgttaat acttatagct ccgtttgaaa catcttcacg tgcttgacgt
    41041 ttagatggtt caatttttgt ggaaacaagc agttccacga tattttcagg tgtgcttgaa
    41101 atttcaacgt tcggcatttt accgaatcct tgcgcgattt cttccgcatt tagttcttta
    41161 atatttccag agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta
    41221 tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct
    41281 gtctctactt ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt aaagaatttc
    41341 aagtatttca ctacatcgcg atcatcttga ttcaaccaga attggtagaa ctcaaatggt
    41401 gaagttttct tcggatcaag ccaaacggca ccgcctgcag tttttccgaa tttcgttccg
    41461 tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat
    41521 tttcggatta ggtctaatcc agctgtgata tttccccatt ggtctgctcc accgatttgc
    41581 aattgcacat cgtatgtttt atgcagatgc aaaaagtcga tagattgcag gatttggtaa
    41641 gtaaactctg taaatgagat tcctacttcc aaacggctgg caacgatatc ttttgctaac
    41701 atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa agagattttt
    41761 gacaaccagt cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa caattttctc
    41821 atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg caacacacgt
    41881 tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat cagaatatat
    41941 ggatgatggc ctgccaattg gaatcttttc atcatcataa atggaatcaa atgtccgata
    42001 tgcatcgaat caccagttgg atcaacgccg caataaagtg aaatgctctt ttcttcaacg
    42061 agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca agttaattca
    42121 tcaataatat tcattttact gacctccgtg tagttttttt atgaaagcag gaacctgcta
    42181 gcatgtgtaa ttaattattt cttcaggata ggataggaaa taaaaaaagt cccttggcat
    42241 ataagccaag ggacgaaatc tcgcggtacc acccaatttg tatgtctaga tatccaacat
    42301 acctctcgaa catttaacgc tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa
    42361 ttcgctttaa agcatgtact ggtttgcagc gaccaccagc tttctgaaac attggcttta
    42421 acaactactt cgtttcgatc ttcgtcttgt tattattaat atgtgtctat catagagcgc
    42481 aactttttct ttgtcaacag aaaaaggata ttttttttaa attcctcatt ttttcttttt
    42541 tgaatccgct ctaattccga acattttatt ttcaattatt gttaaaagaa ataacttttt
    42601 ctgtattttt tctgcaattt atcgagaagt cactttactt tgcctcttat tctttcttcg
    42661 ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa
    42721 aaaagcaaaa aattatttta ggagccactt ctgctttaac tatcgttatt ttaattctaa
    42781 gtttgttttt tgtcatccga tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa
    42841 taacagaaaa agcaaaagaa ccgacagatg ataagcaaca actagttact caagcaaaaa
    42901 atctggctca aagctacgat tatgaccaag cgattgcgtt attggaaaaa gatagtaaga
    42961 acggatctac tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa gaacaactga
    43021 tcgactggtc tgatcctact cagatctctc atgtcttttt ccacagcttg atcgtggacc
    43081 cgcaaaaagc cttccactct caacaggcac aaggatacaa agattatatg gtgactgttg
    43141 aagaattcaa ccgttcgatc gaacaacttt atcaaaatga ttttgtttta gttaatttga
    43201 atgatttgat ccaaaaagac gaacaaggaa atctttcctt taacgggctt gcgctacctg
    43261 aaggaaaaaa accattggtt ctttcacaag atgatgtcag ctattatgaa tatatggata
    43321 attccggttt tcctagccga ttggtcgtat caaataacaa tgaaatcaaa aatgtctata
    43381 aagagaaaga taaagaagaa atcggtgact ttgacatggt tcctctgatc gatgcctttg
    43441 tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt
    43501 acaacggagt tttaggttat cgaacatcaa aatctcaata tggagacaat gaaaaaacaa
    43561 aaaaagagat taaagaagct aaaaaagtgg cggaacagtt gaagaaagac ggctggagct
    43621 ttgcttccca tacatggggt cacttgaata tgactcaagc ttcactagaa gatgtaaaaa
    43681 aagacaatga attgtggcag caagaagttg cacctatcct aggaaaaaca aatattctga
    43741 tttatccatt tggtgcagac atcagcgact ggcagcctta ttcagcagaa aatcaaaaat
    43801 ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct tctacaccag
    43861 cttggggaca attaggtacg gatttctatc gaaatgctcg gatcaatatc gatggcattc
    43921 gttttcaatc tgatttagac ggaaaaaatc ctattcttga tccattcatc aatgtccatg
    43981 atgtctatga caaagaagca cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa
    44041 agccgaactg acattttatc gtcagtttcg gcttttttat gctttatttc ttcttcggtg
    44101 cgattccctt gatcaggact tctgtccatt ctgctgtata tggaagcagt tttgcgtata
    44161 tctccggctt agaaattcct tctaccattg cttgaaaata gaggatcaag aactcatccg
    44221 aatatttcaa atccactttt ccttctttcc gccctcgatg gaaaagatca agcatgatac
    44281 tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt tctttttttg
    44341 tatagtatgt catcaaatcg atataaaatt gtttgttcac tcgattgaga taacggattt
    44401 tgttttcact catggcaatc agtgtttctt caaatggaag attttccgca atcaacgctt
    44461 tcgcttcttg ccctaaatca ttcataaatg caaaaaatac ttcataaatc agcccttcct
    44521 tgctgccaaa atatttaaaa atcgtcgctt tacttacctg agcgttctca gctaccttat
    44581 ccattgtcac tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa
    44641 tctctttctt tttctcttgt gctctctttt caaaaccgtt catttgtaga gcctcccgta
    44701 attttaaact tttataatat atatagttca aatttattct gaggtttatt ataactatct
    44761 attgagaaag tatcaagcta gcaattcctt gtttatgaat tcattgacag aaatttataa
    44821 aaagaatatt atgtactaaa ataataaaaa aagttttata aaaaggtata aaaccttttt
    44881 attaaactat ttaatcttgt ttagtatata tttttagaaa aaggagtggg gtttttatgg
    44941 cagaaatcgt caaagtcaca catttgaaaa agagctttgg tcgtttcgaa gctttaaaag
    45001 atgtctcttt caccgtacat tcaggtgaag tcgttggatt tatcggacca aatggttctg
    45061 gaaaatccac gacgattcgg acacttttag ggattataaa aaaagacggc ggaacggcaa
    45121 cgatttttgg gcaagatgta tgggaagata gtttatctat ccatgaacgt ctttcttatg
    45181 ttccaggcga tatctctttg tggggaaatt tgactggagg agcgatcatc gatctcttca
    45241 tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga actgattcaa cgtttctctc
    45301 ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga
    45361 ttgctgcttt ggctgttgat tcggatctct atttatttga tgagcctact tctggtttag
    45421 atcctttgat ggaaacgatt ttccaagaag aagttgaaaa attgaaagaa cagggcaaat
    45481 ccatcctact ttcttctcat attctcagtg aagtcgagcg actagcggat aaagtcgtga
    45541 tcatacgcga aggaacactc gatgaagaag gaacactcga tgaattgcgt catttgactc
    45601 gttcgaccat cactatagaa acagaagcag agaatagtca cttagcacaa ttgaacggtg
    45661 tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc tgctgataac gaagctttcg
    45721 atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa atttgaatct attccaccga
    45781 ctcttgagga tttattcatg agacattatg aaggatgagg aggaagcgaa atgactgata
    45841 aattttcacg ctggtccacc cttttttctc aaaaccttag ccgtgactgg aaaaagatcc
    45901 tgatatggct agtcggtgtc actgcttttt ctggtggttt tgtccctgcg tttgaagaat
    45961 tgacttccgg acaaggatta gctggattat acgaaacctt gcaaaacccg gcaatgattg
    46021 caatggtcgg accaactcca gcttcgaatg ctgctgagta tactttagga gcgatgtatg
    46081 caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg
    46141 tgagccaaac gagaaaaaaa gaagaacttg gtctgattga acttattcgt tcctttcgag
    46201 tcggacgtca agcaaactct ttagctgtca tcgctgaaag tattctgatc aatttgcttt
    46261 tagcaatatc gatcagttgt acgatgattt ttttccaagc ggattcgatc actgcagaag
    46321 gtgcttggct ttttgggcta tctgtaggta gtatggggat tttggggagt gccgttgcac
    46381 tgtttgctgc acaactgatg ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt
    46441 taggtggact ttaccttcta cgagcaataa cagatgtagc tgattcctcc ctttcgatgt
    46501 ggaatccttt aggctggtct tatttgacct atccatttac aactaacaac tggattcctt
    46561 tgctttattt gtttttgttc agtctaatct tgataatcag ttcatttgtt ttagaggaac
    46621 atcgagattt gaacaccggc tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt
    46681 ctcttttatc cttgcgtggt ttattattct atctaaataa agggatgaca atcagttggc
    46741 ttatcacttt tggtttgatg ggggcggcat acggctctat ctacgggaat atgcaacagt
    46801 ttttagaaag taacgactta atgaaacaga tgtttatcca atctggcgtc tcaattgaaa
    46861 aaaactttac aagcacgatc atggtcgtct tgatttgtct agctgctatt ttaccaattg
    46921 ctagcatcaa tcgtttattt acagaagaaa cccacggacg tatgaatcag ctgttcgcaa
    46981 caaaaatttc taggaaccaa ctttattgga ccaatatgtt actagctatt ctatttgcta
    47041 tattggcaat cctgattagt tctggtgcac tagggctttc tgctttggct tcgatgaagg
    47101 atgcgaaaga catggatcta ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg
    47161 ttttagtgtt tatcggaatt agtggctttc tattcggatg gcttcctcga atcggtaaac
    47221 ttgcttatat ctacttagct tactcctttg cactcaatta ctttggcgga attttggatt
    47281 taccagaatg gttttccaaa acagctgtcc ctagctgggt tccacggatg ccaaacgatt
    47341 cttttgatct ttccgtgttc cttacgttat catgtatcag ccttgcactg ttcgtccttg
    47401 gttctattgg ttataggcga agagacttac tagaaggata ataaaaaagc atgtacaatt
    47461 tcatgctagc ctccaaatct gggtcaaaaa tccaacattt ggaggtcatt tttttattta
    47521 caagtcttct aactgctctt tatcaatgat ccagacttct cgattctttc tttttacata
    47581 accattctcc tctaataatt taaattttct agataaagtt tcgggggtcg taccgataaa
    47641 tgcagcaagt tctttcattt tcattggcag gaaaaagtga tccttccctg tcgctttagc
    47701 taagtcaagc agatagacag cgactcgttc ttctactttt tccatcatca aaaaagttgt
    47761 ctgctgctgt acttttgtta atttctcaac gttgattgct aaaagtttca gactaagggc
    47821 gggataatgc agtaagagtt ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt
    47881 ttctagtgct tcgccaaata aagactcatt tggaacgcct agcaactggc tctctccctc
    47941 atatccgccc ggctctatta ctcgtaaaag ctgttccttt ccgctagcag ataattgata
    48001 cactttcata ctgccggagg ccacgatcac taattgaggt tctccattcg gcgcaagaat
    48061 ctgttctcct ttttccacat agtgatgttt gacaagtgcg tgtacttttt cttgatcttg
    48121 ctgtactaag tgattgaaca aaggaacaaa tgaaacacat aaatgatcat gactcataat
    48181 ctctcctcct tctaaacagc tatttctaat aaagaaacat aacttccctt attccaactg
    48241 ataattttct tttatcatag cataaacgtc aagcgaacca gataccagca aatagagtgg
    48301 atttcttttt gtcccttgct ttattttcct tcttttgaga ccttaagtaa ttgattccta
    48361 tcaatttttt aaatatttac tttttgaata atcaaaaaca aaccttcact cgcaagccga
    48421 gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc tattgaaatt tttccgcacc
    48481 tagcttttga tatagtggtg ctagttcttg taaggtttca gcggttattt gctcaacgtg
    48541 ttcgatttct tgcggataga agaatagttt acccatttga aaggttcggt ctctcatcgc
    48601 ttttttcaac tcttcttgtt tctttgtatc ggacaaatag tcagacaaag ctaagtggtc
    48661 gtcaagttca tgttctggtt gcggccatat gtaataatcc ttgatatcga ctgttttgct
    48721 ccactctttc agtatcgcaa gcaactggtt atacgctgca atgctaccat atcgttcttc
    48781 gcttttataa tgttggtaca tcaaatagat ttggtattgt ttttgattca acaaaactgc
    48841 tagacaggca ttctcgtctt gacgagattc gcttcggtac gcgctccaaa aatgactgcg
    48901 taaattccag ccattcgtcc agctttcgat tttaggtttg ctcatgccgt acatatcagg
    48961 caaaagtgca gctgtctgga gctgaatctc tttccatttt tgccagtgtt ctttatattc
    49021 tttcttgatt tgttcaatct tatctgttgg atggctctct ttgagctgtt tgaatgcgta
    49081 gattggtcga tcaaacaact gataatctga ttttccaagt tccattgttt cgcctctttc
    49141 cgttatcttt tgtattctac ctgaattttg cgatatggac aaatagaggt tgtgaaaaag
    49201 ctgttctgca tcaagtcata agaacagcca aacaaaaaac tgcgatgagc atcaccgcag
    49261 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
    49321 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
    49381 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
    49441 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
    49501 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
    49561 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
    49621 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
    49681 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
    49741 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
    49801 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
    49861 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
    49921 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
    49981 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
    50041 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
    50101 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
    50161 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
    50221 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
    50281 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
    50341 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
    50401 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
    50461 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
    50521 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
    50581 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
    50641 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
    50701 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
    50761 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
    50821 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
    50881 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
    50941 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
    51001 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
    51061 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
    51121 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
    51181 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
    51241 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
    51301 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
    51361 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
    51421 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
    51481 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
    51541 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
    51601 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
    51661 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
    51721 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
    51781 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
    51841 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
    51901 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
    51961 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
    52021 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
    52081 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
    52141 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
    52201 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
    52261 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
    52321 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
    52381 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
    52441 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
    52501 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
    52561 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
    52621 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
    52681 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
    52741 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
    52801 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
    52861 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
    52921 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
    52981 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
    53041 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
    53101 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
    53161 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
    53221 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
    53281 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
    53341 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
    53401 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
    53461 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
    53521 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
    53581 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
    53641 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
    53701 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
    53761 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
    53821 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
    53881 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
    53941 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
    54001 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
    54061 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
    54121 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
    54181 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
    54241 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
    54301 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
    54361 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
    54421 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
    54481 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
    54541 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
    54601 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
    54661 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
    54721 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
    54781 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
    54841 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
    54901 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
    54961 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
    55021 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
    55081 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
    55141 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
    55201 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
    55261 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
    55321 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
    55381 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
    55441 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
    55501 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
    55561 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
    55621 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
    55681 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
    55741 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
    55801 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
    55861 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
    55921 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
    55981 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
    56041 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
    56101 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
    56161 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
    56221 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
    56281 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
    56341 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
    56401 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
    56461 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
    56521 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
    56581 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
    56641 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
    56701 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
    56761 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
    56821 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
    56881 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
    56941 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
    57001 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
    57061 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
    57121 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
    57181 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
    57241 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
    57301 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
    57361 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
    57421 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
    57481 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
    57541 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
    57601 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
    57661 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
    57721 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
    57781 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
    57841 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
    57901 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
    57961 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
    58021 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
    58081 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
    58141 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
    58201 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
    58261 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
    58321 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
    58381 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
    58441 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
    58501 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
    58561 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
    58621 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
    58681 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
    58741 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
    58801 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
    58861 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
    58921 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
    58981 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
    59041 acctcatggg gtatattaaa caggagcttt attaatatgt caaccaaaat gactcttcat
    59101 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
    59161 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
    59221 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    59281 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    59341 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    59401 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    59461 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    59521 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    59581 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    59641 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    59701 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    59761 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    59821 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    59881 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    59941 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    60001 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    60061 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    60121 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    60181 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    60241 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    60301 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    60361 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    60421 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    60481 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    60541 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    60601 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    60661 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    60721 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    60781 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    60841 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    60901 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    60961 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    61021 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    61081 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    61141 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    61201 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    61261 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    61321 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    61381 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    61441 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    61501 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    61561 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    61621 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    61681 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    61741 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    61801 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    61861 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    61921 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    61981 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    62041 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    62101 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    62161 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    62221 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    62281 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    62341 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    62401 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    62461 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    62521 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    62581 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    62641 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    62701 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    62761 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    62821 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    62881 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    62941 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    63001 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    63061 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    63121 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    63181 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    63241 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    63301 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    63361 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    63421 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    63481 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    63541 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    63601 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    63661 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    63721 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    63781 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    63841 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    63901 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    63961 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    64021 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    64081 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    64141 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    64201 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    64261 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    64321 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    64381 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    64441 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    64501 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    64561 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    64621 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    64681 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    64741 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    64801 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    64861 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    64921 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    64981 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    65041 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    65101 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    65161 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    65221 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    65281 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    65341 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    65401 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    65461 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    65521 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    65581 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    65641 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    65701 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    65761 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    65821 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    65881 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    65941 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    66001 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    66061 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    66121 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    66181 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    66241 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    66301 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    66361 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    66421 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    66481 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    66541 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    66601 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    66661 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    66721 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    66781 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    66841 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    66901 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    66961 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    67021 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    67081 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    67141 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    67201 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    67261 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    67321 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    67381 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    67441 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    67501 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    67561 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    67621 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    67681 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    67741 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    67801 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    67861 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    67921 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    67981 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    68041 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    68101 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    68161 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    68221 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    68281 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    68341 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    68401 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    68461 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    68521 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    68581 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    68641 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    68701 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    68761 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    68821 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    68881 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    68941 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    69001 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    69061 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    69121 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    69181 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    69241 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    69301 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    69361 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    69421 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    69481 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    69541 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    69601 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    69661 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    69721 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    69781 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    69841 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    69901 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    69961 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    70021 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    70081 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    70141 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    70201 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    70261 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    70321 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    70381 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    70441 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    70501 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    70561 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    70621 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    70681 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    70741 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    70801 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    70861 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    70921 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    70981 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    71041 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    71101 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    71161 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    71221 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    71281 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    71341 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    71401 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttctt caaaaggttt
    71461 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    71521 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    71581 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    71641 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    71701 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    71761 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    71821 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    71881 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    71941 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    72001 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    72061 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    72121 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    72181 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    72241 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    72301 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    72361 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    72421 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    72481 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    72541 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    72601 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    72661 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    72721 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    72781 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    72841 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    72901 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    72961 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    73021 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    73081 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    73141 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    73201 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    73261 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    73321 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    73381 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    73441 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    73501 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    73561 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    73621 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    73681 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    73741 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    73801 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    73861 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    73921 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    73981 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    74041 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    74101 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    74161 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    74221 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    74281 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    74341 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    74401 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    74461 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    74521 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    74581 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    74641 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    74701 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    74761 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    74821 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    74881 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    74941 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    75001 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    75061 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    75121 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    75181 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    75241 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    75301 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    75361 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    75421 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    75481 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    75541 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    75601 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    75661 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    75721 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    75781 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    75841 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    75901 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    75961 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    76021 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    76081 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    76141 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    76201 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    76261 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    76321 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    76381 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    76441 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    76501 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    76561 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    76621 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    76681 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    76741 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    76801 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    76861 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    76921 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    76981 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    77041 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    77101 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    77161 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    77221 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    77281 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    77341 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    77401 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    77461 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    77521 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    77581 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    77641 aagcgctttc atctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    77701 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    77761 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    77821 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    77881 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    77941 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    78001 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    78061 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    78121 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    78181 gcatgatatt ggaaaaaatt gtactgattt gttgaggcaa ccttgcgtgt tggatcaccg
    78241 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    78301 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    78361 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    78421 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    78481 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    78541 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    78601 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    78661 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    78721 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    78781 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    78841 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    78901 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    78961 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    79021 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    79081 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    79141 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    79201 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    79261 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    79321 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    79381 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    79441 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    79501 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    79561 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    79621 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    79681 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    79741 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    79801 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    79861 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    79921 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    79981 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    80041 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    80101 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    80161 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    80221 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    80281 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    80341 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    80401 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    80461 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    80521 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    80581 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    80641 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    80701 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    80761 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    80821 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    80881 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    80941 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    81001 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    81061 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    81121 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    81181 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    81241 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    81301 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    81361 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    81421 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    81481 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    81541 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    81601 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    81661 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    81721 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    81781 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    81841 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    81901 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    81961 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    82021 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    82081 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    82141 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    82201 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    82261 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    82321 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    82381 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    82441 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    82501 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    82561 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    82621 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    82681 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    82741 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    82801 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    82861 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    82921 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    82981 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    83041 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    83101 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    83161 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    83221 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    83281 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    83341 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    83401 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    83461 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    83521 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    83581 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    83641 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    83701 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    83761 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    83821 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    83881 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    83941 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    84001 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    84061 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    84121 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    84181 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    84241 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    84301 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    84361 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    84421 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    84481 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    84541 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    84601 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    84661 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    84721 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    84781 tttacaaaaa aaaaaaacgg caatgaatgc cgctcttctt atttcgattc ttttataatt
    84841 ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca aatgtttgat
    84901 cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt tcagcattga
    84961 tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt
    85021 cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg atttcttccg
    85081 gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca ccttgggctt
    85141 ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa
    85201 aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa aattcccgtt
    85261 cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt ttagtcttga
    85321 atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat gtttcttctg
    85381 taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac gtttcttcac
    85441 tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg gttgcttcag
    85501 gaaatacttc ccagaaattg gtatccacta ttactttttt catctgttca cacctcttga
    85561 ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca tataaaaaac
    85621 atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa catcgttgtg
    85681 ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt caatagctgt
    85741 atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat agattcgctc
    85801 gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat ttagtttcag
    85861 gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt tatcttttag
    85921 ctcttctgta agtttttcta gtgcataagc ttgtgtcaag taatcagcta aatgctccgg
    85981 tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc
    86041 tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt cctttcaata
    86101 gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct tgcatacttt
    86161 atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta atgcgactgc
    86221 attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt ttttagtcgg
    86281 atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca tcccattgga
    86341 ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca tttctgctag
    86401 tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat actgtcctct
    86461 tgttaaatcg acttctttga attcgatatt gctaatagaa tctagcgctc gggcaatcat
    86521 tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt gtaagactag
    86581 ttcacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt tcttttttaa
    86641 taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca atagatctgg
    86701 tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga tagctttggc
    86761 aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat aaacttggcc
    86821 tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag ctgtggaata
    86881 tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac tgttaatcct
    86941 gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat ttcagcaaat
    87001 ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac cttgccatct
    87061 tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc aatcgttgtg
    87121 agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc caatgaaatc
    87181 gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata tagaccagct
    87241 tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct cttcccttct
    87301 tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctggtttaat tacggcaaat
    87361 aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa caaaagacat
    87421 tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc ggtaataagc
    87481 cccaccaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt aattgaggca
    87541 ggacagcttt ttcacaacct ccccactaaa cggtgttctc ctaccaactc ctcggtcaca
    87601 agtcacaata ttcatcaaac atgagaagct gtttttttga aattgctcct tgtgactcgg
    87661 agctgaacga caggctcacg acctctcggt aaacagcgtt caaaagccaa tttttcgata
    87721 ttaagccatt tctttgttac ttattgtcat tgatttcttt ttctaattta tccaatagtg
    87781 gagtgtaatc cacttgagta tagtctgtta catcaaattc atatagtgtt cctaactgga
    87841 atttgtcata ttttcggtcg gcgatgatct ttttgaatgc ttctttcgta atatgattgg
    87901 tccctttcgt tcgatcttcc aatacgtcac catagtgata atgatccatg atatagctca
    87961 aatgacgatc tgattcacga tcacgtttgt accgacgttg ccataacact tcaaagtctg
    88021 ctgttaggcg aatcgtgatc acctgatatc cgtatctatc tgctaattct tggagtcgtg
    88081 ctttttgctt atcactaaaa ggatactcgg acacaatcac tttctttcct gcttccatat
    88141 acaaatcaag tgctttgtaa tagaaaggat aaactttcgt cctttcttgt tccgcccgtt
    88201 cttccaaatt gttaaacccg atggattctg catagtattc cttgatttca tcgagcgtga
    88261 tggcatacat atctggaaat cgttcccgta gtttattcat aagatatgtt tttcctgtac
    88321 caggactacc agccagaaga atgaaatact ttttcatctc atttatttcc taagttaatt
    88381 ggactttcca agttcataaa cagtccaatc ttatgcttga caatttcttt tactgcttca
    88441 tttgctggaa tcacattatg gcgaagagac ttgttgattt ctgtttcact aaaggtttct
    88501 ttagctactt tcgtccagtt gactaaaagc tccgttccaa cattgaattt gcggatcccg
    88561 ttattgatca attcaggata atcttcttct ttcacacctg ttcctccatg gataactagg
    88621 gggacatcaa ctactgcatc gatttctttc agcaagggaa tgtttacttc tgtttttgat
    88681 ttgaattgtc catgattcgt accgatagca atagctaatg catccacgcc tgtagcttct
    88741 acaaatttca ctgcgtcttc cggtcttgta tataccttgt cattttcagc gacatggatt
    88801 ccttcttctg taccgccgat tgttccaagt tctccttcta cggaaacacc gtgcgcatgg
    88861 gcatattcta ctactgcttt tgtcttcagg atattttctt tgaaaggcag atgggaccca
    88921 tcatacatga cagaagtata tccgctgtcg attgcttctt tgatatcatc aaagtctcgg
    88981 gcgtggtcta agtgcaagac tgcatcaacc atttcttctt ctgcaatagc tgtcattaca
    89041 ttgactaaca catgcatgcc gatatattta gcggtatcga cacttgtttg aataatgatc
    89101 ggcgaaccca tttcttttgc tgcgcctagc atagcaggaa gcatttctag gttatgcgta
    89161 ttaaaagctc caactgtaaa attcaactct tctgctgttt ttgttacttc tttcaatgtt
    89221 gtatacatct tttattgcca ccttatcttt ttatttgttt cctttgatcg ttacttgttt
    89281 tgcaatttgt cgcatctcat ccattttgcc catgtagtaa tcgatccctt gatcatcacc
    89341 agcacgagca gcatctgcac gcttctggtt atagaggctc atcagttttt tatacccatc
    89401 tccgatcgtc tgtttatttt ccaaactctt ttctaaatat tggatggctt gatcgttcac
    89461 atacaattca gaaaatttaa gtccaacttg ttcataaagt ttcgctaatt tatgcttatc
    89521 ttgggtagtg gaaatctctt gtttcaattg tgtaatctct gattgaatct tttcggtcgc
    89581 ttctggcgat agggcttttt cttcttttgc cagaactttt ttcggttctt tttcttt
//
LOCUS       DADXTH010000002        88721 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.002, whole genome shotgun sequence.
ACCESSION   DADXTH010000002 DADXTH010000000
VERSION     DADXTH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.002"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(414..1196)
                     /locus_tag="IWI97_000081"
     CDS             complement(414..1196)
                     /locus_tag="IWI97_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461624.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1261..2097)
                     /locus_tag="IWI97_000082"
     CDS             complement(1261..2097)
                     /locus_tag="IWI97_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2461625.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2115..3524)
                     /locus_tag="IWI97_000083"
     CDS             complement(2115..3524)
                     /locus_tag="IWI97_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2461626.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3538..5361)
                     /locus_tag="IWI97_000084"
     CDS             complement(3538..5361)
                     /locus_tag="IWI97_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2461627.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5641..5868)
                     /locus_tag="IWI97_000085"
     CDS             complement(5641..5868)
                     /locus_tag="IWI97_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461628.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(6204..6530)
                     /locus_tag="IWI97_000086"
     CDS             complement(6204..6530)
                     /locus_tag="IWI97_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461629.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6576..6938)
                     /locus_tag="IWI97_000087"
     CDS             complement(6576..6938)
                     /locus_tag="IWI97_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBC2461630.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6975..7190)
                     /locus_tag="IWI97_000088"
     CDS             complement(6975..7190)
                     /locus_tag="IWI97_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2461631.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7317..7652
                     /locus_tag="IWI97_000089"
     CDS             7317..7652
                     /locus_tag="IWI97_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2461632.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7728..9725)
                     /locus_tag="IWI97_000090"
     CDS             complement(7728..9725)
                     /locus_tag="IWI97_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461633.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9823..10098)
                     /locus_tag="IWI97_000091"
     CDS             complement(9823..10098)
                     /locus_tag="IWI97_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2461634.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10330..11064
                     /locus_tag="IWI97_000092"
     CDS             10330..11064
                     /locus_tag="IWI97_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2461635.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11103..12245
                     /locus_tag="IWI97_000093"
     CDS             11103..12245
                     /locus_tag="IWI97_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2461636.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12340..13731)
                     /gene="sufB"
                     /locus_tag="IWI97_000094"
     CDS             complement(12340..13731)
                     /gene="sufB"
                     /locus_tag="IWI97_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBC2461637.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13736..14206)
                     /locus_tag="IWI97_000095"
     CDS             complement(13736..14206)
                     /locus_tag="IWI97_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBC2461638.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14193..15428)
                     /locus_tag="IWI97_000096"
     CDS             complement(14193..15428)
                     /locus_tag="IWI97_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBC2461639.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15425..16711)
                     /gene="sufD"
                     /locus_tag="IWI97_000097"
     CDS             complement(15425..16711)
                     /gene="sufD"
                     /locus_tag="IWI97_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBC2461640.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16724..17497)
                     /gene="sufC"
                     /locus_tag="IWI97_000098"
     CDS             complement(16724..17497)
                     /gene="sufC"
                     /locus_tag="IWI97_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBC2461641.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17694..18542)
                     /locus_tag="IWI97_000099"
     CDS             complement(17694..18542)
                     /locus_tag="IWI97_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBC2461642.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18566..19252)
                     /locus_tag="IWI97_000100"
     CDS             complement(18566..19252)
                     /locus_tag="IWI97_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2461643.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19245..20279)
                     /locus_tag="IWI97_000101"
     CDS             complement(19245..20279)
                     /locus_tag="IWI97_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2461644.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20342..20598)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20674..21012)
                     /locus_tag="IWI97_000102"
     CDS             complement(20674..21012)
                     /locus_tag="IWI97_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBC2461645.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21014..21370)
                     /locus_tag="IWI97_000103"
     CDS             complement(21014..21370)
                     /locus_tag="IWI97_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBC2461646.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21376..22563)
                     /locus_tag="IWI97_000104"
     CDS             complement(21376..22563)
                     /locus_tag="IWI97_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBC2461647.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22754..23599)
                     /locus_tag="IWI97_000105"
     CDS             complement(22754..23599)
                     /locus_tag="IWI97_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2461648.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23833..24798
                     /locus_tag="IWI97_000106"
     CDS             23833..24798
                     /locus_tag="IWI97_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBC2461649.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25175..26218
                     /locus_tag="IWI97_000107"
     CDS             25175..26218
                     /locus_tag="IWI97_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBC2461650.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26280..26744)
                     /locus_tag="IWI97_000108"
     CDS             complement(26280..26744)
                     /locus_tag="IWI97_000108"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBC2461651.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26990..27937
                     /locus_tag="IWI97_000109"
     CDS             26990..27937
                     /locus_tag="IWI97_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2461652.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28148..30220)
                     /locus_tag="IWI97_000110"
     CDS             complement(28148..30220)
                     /locus_tag="IWI97_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBC2461653.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30153..30530)
                     /locus_tag="IWI97_000111"
     CDS             complement(30153..30530)
                     /locus_tag="IWI97_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBC2461654.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30659..31237)
                     /locus_tag="IWI97_000112"
     CDS             complement(30659..31237)
                     /locus_tag="IWI97_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2461655.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31369..32661
                     /locus_tag="IWI97_000113"
     CDS             31369..32661
                     /locus_tag="IWI97_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBC2461656.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32721..33839)
                     /locus_tag="IWI97_000114"
     CDS             complement(32721..33839)
                     /locus_tag="IWI97_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBC2461657.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33853..35274)
                     /gene="arcD"
                     /locus_tag="IWI97_000115"
     CDS             complement(33853..35274)
                     /gene="arcD"
                     /locus_tag="IWI97_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBC2461658.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35520..36641)
                     /gene="mnmA"
                     /locus_tag="IWI97_000116"
     CDS             complement(35520..36641)
                     /gene="mnmA"
                     /locus_tag="IWI97_000116"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBC2461659.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37045..37644
                     /locus_tag="IWI97_000117"
     CDS             37045..37644
                     /locus_tag="IWI97_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBC2461660.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37782..39068)
                     /gene="efmA"
                     /locus_tag="IWI97_000118"
     CDS             complement(37782..39068)
                     /gene="efmA"
                     /locus_tag="IWI97_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBC2461661.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39339..39683)
                     /locus_tag="IWI97_000119"
     CDS             complement(39339..39683)
                     /locus_tag="IWI97_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBC2461662.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39820..40965)
                     /locus_tag="IWI97_000120"
     CDS             complement(39820..40965)
                     /locus_tag="IWI97_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBC2461663.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(41145..42119)
                     /locus_tag="IWI97_000121"
     CDS             complement(41145..42119)
                     /locus_tag="IWI97_000121"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBC2461664.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(42199..43074)
                     /locus_tag="IWI97_000122"
     CDS             complement(42199..43074)
                     /locus_tag="IWI97_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBC2461665.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            43204..45111
                     /locus_tag="IWI97_000123"
     CDS             43204..45111
                     /locus_tag="IWI97_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBC2461666.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(45652..48930)
                     /locus_tag="IWI97_000124"
     CDS             complement(45652..48930)
                     /locus_tag="IWI97_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBC2461667.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(49201..49746)
                     /locus_tag="IWI97_000125"
     CDS             complement(49201..49746)
                     /locus_tag="IWI97_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2461668.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(49758..50450)
                     /locus_tag="IWI97_000126"
     CDS             complement(49758..50450)
                     /locus_tag="IWI97_000126"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBC2461669.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(50469..50753)
                     /locus_tag="IWI97_000127"
     CDS             complement(50469..50753)
                     /locus_tag="IWI97_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461670.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(50766..51329)
                     /locus_tag="IWI97_000128"
     CDS             complement(50766..51329)
                     /locus_tag="IWI97_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBC2461671.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            51557..52219
                     /locus_tag="IWI97_000129"
     CDS             51557..52219
                     /locus_tag="IWI97_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBC2461672.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            52343..53539
                     /locus_tag="IWI97_000130"
     CDS             52343..53539
                     /locus_tag="IWI97_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBC2461673.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(53640..55016)
                     /locus_tag="IWI97_000131"
     CDS             complement(53640..55016)
                     /locus_tag="IWI97_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2461674.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(55053..55919)
                     /locus_tag="IWI97_000132"
     CDS             complement(55053..55919)
                     /locus_tag="IWI97_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBC2461675.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(56008..57033)
                     /locus_tag="IWI97_000133"
     CDS             complement(56008..57033)
                     /locus_tag="IWI97_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBC2461676.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(57051..57725)
                     /locus_tag="IWI97_000134"
     CDS             complement(57051..57725)
                     /locus_tag="IWI97_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2461677.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(57909..58802)
                     /locus_tag="IWI97_000135"
     CDS             complement(57909..58802)
                     /locus_tag="IWI97_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2461678.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(58876..60573)
                     /locus_tag="IWI97_000136"
     CDS             complement(58876..60573)
                     /locus_tag="IWI97_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBC2461679.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(60566..61879)
                     /locus_tag="IWI97_000137"
     CDS             complement(60566..61879)
                     /locus_tag="IWI97_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBC2461680.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(61879..65397)
                     /locus_tag="IWI97_000138"
     CDS             complement(61879..65397)
                     /locus_tag="IWI97_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBC2461681.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(65690..66148)
                     /locus_tag="IWI97_000139"
     CDS             complement(65690..66148)
                     /locus_tag="IWI97_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBC2461682.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(66445..66963)
                     /locus_tag="IWI97_000140"
     CDS             complement(66445..66963)
                     /locus_tag="IWI97_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBC2461683.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(66965..67582)
                     /locus_tag="IWI97_000141"
     CDS             complement(66965..67582)
                     /locus_tag="IWI97_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBC2461684.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(67579..68433)
                     /locus_tag="IWI97_000142"
     CDS             complement(67579..68433)
                     /locus_tag="IWI97_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2461685.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(68430..69341)
                     /locus_tag="IWI97_000143"
     CDS             complement(68430..69341)
                     /locus_tag="IWI97_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2461686.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(69382..70716)
                     /locus_tag="IWI97_000144"
     CDS             complement(69382..70716)
                     /locus_tag="IWI97_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2461687.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            70924..71754
                     /locus_tag="IWI97_000145"
     CDS             70924..71754
                     /locus_tag="IWI97_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBC2461688.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(71998..72280)
                     /locus_tag="IWI97_000146"
                     /pseudo
     CDS             complement(71998..72280)
                     /locus_tag="IWI97_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(72438..72632)
                     /locus_tag="IWI97_000147"
     CDS             complement(72438..72632)
                     /locus_tag="IWI97_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461689.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(73137..73322)
                     /locus_tag="IWI97_000148"
     CDS             complement(73137..73322)
                     /locus_tag="IWI97_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461690.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(74172..74513)
                     /locus_tag="IWI97_000149"
     CDS             complement(74172..74513)
                     /locus_tag="IWI97_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBC2461691.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(74513..74743)
                     /locus_tag="IWI97_000150"
     CDS             complement(74513..74743)
                     /locus_tag="IWI97_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBC2461692.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            75030..75368
                     /locus_tag="IWI97_000151"
     CDS             75030..75368
                     /locus_tag="IWI97_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461693.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(75573..75812)
                     /locus_tag="IWI97_000152"
     CDS             complement(75573..75812)
                     /locus_tag="IWI97_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBC2461694.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(75788..76135)
                     /locus_tag="IWI97_000153"
     CDS             complement(75788..76135)
                     /locus_tag="IWI97_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBC2461695.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(76867..77448)
                     /locus_tag="IWI97_000154"
     CDS             complement(76867..77448)
                     /locus_tag="IWI97_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2461696.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(77631..78257)
                     /locus_tag="IWI97_000155"
     CDS             complement(77631..78257)
                     /locus_tag="IWI97_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2461697.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(78279..79610)
                     /locus_tag="IWI97_000156"
     CDS             complement(78279..79610)
                     /locus_tag="IWI97_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBC2461698.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(79765..80166)
                     /locus_tag="IWI97_000157"
     CDS             complement(79765..80166)
                     /locus_tag="IWI97_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBC2461699.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYPKATRGNIDVEVTIV"
     gene            complement(80252..80458)
                     /locus_tag="IWI97_000158"
     CDS             complement(80252..80458)
                     /locus_tag="IWI97_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461700.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(80553..82055)
                     /locus_tag="IWI97_000159"
     CDS             complement(80553..82055)
                     /locus_tag="IWI97_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBC2461701.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(82073..82528)
                     /gene="argR"
                     /locus_tag="IWI97_000160"
     CDS             complement(82073..82528)
                     /gene="argR"
                     /locus_tag="IWI97_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBC2461702.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(82626..83243)
                     /locus_tag="IWI97_000161"
     CDS             complement(82626..83243)
                     /locus_tag="IWI97_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBC2461703.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            83464..84837
                     /locus_tag="IWI97_000162"
     CDS             83464..84837
                     /locus_tag="IWI97_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2461704.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            84912..86024
                     /locus_tag="IWI97_000163"
     CDS             84912..86024
                     /locus_tag="IWI97_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBC2461705.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(86096..87733)
                     /locus_tag="IWI97_000164"
     CDS             complement(86096..87733)
                     /locus_tag="IWI97_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBC2461706.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(87901..88509)
                     /locus_tag="IWI97_000165"
     CDS             complement(87901..88509)
                     /locus_tag="IWI97_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2461707.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        25516 a        18719 c        15515 g        28971 t
ORIGIN      
        1 tattcttgct aataaactac gcgaaaggag tctaacgttg tttgatataa cttagactga
       61 tcgttaactt attttttata tttctacata gttatcttta gaaccatttc agatacatat
      121 gaaacaatta catatgattt tttcataaaa aaacagtact gtctagtagt ataaaatata
      181 caaatttctt aaaaattttt tcactaaaat cactacctga aacagtctaa aacacttata
      241 tatcaacgtt taatgaaact atctccgttt catttcagat acaaataaat atagtcgggc
      301 tccaaatccg aaaaaaaata cccccagtag cttatcctaa gctactggag atgtataagt
      361 acggtcatat aacaacgggg tagggtaaat aaatgaaaaa gatggaagaa tcattattca
      421 ataacttttt taaatacttc gttgtaatca gttacctgtc cgttcttaaa taccacagaa
      481 taatcttttt tcccattttc aagtaaccga agtggaatgt ctttaccaac ttgctctgtc
      541 actgcattac tgatacctgt tagattagct aataaatctg ataaagattg agcatctgta
      601 gaaatctctg tatcagtatt tatcgtgatt cctttatctt catttacaag cttccaagta
      661 ggtggtatag ctgtgtatgt ttcgatgaat gaatcaattc tttcatctat cgtcttccca
      721 gtatctactt ctgtctcatt actcttagat gaatcgattc tttcatccat cgtctgctcc
      781 gtaactgctt ctgtctcatt attctcaccg gaaactctct tcacacccaa acttatatat
      841 tgtccatctt tagtgtgcat agttaaaatt cccacgcttt tactaataaa ttcactataa
      901 ccagttcttt caatagagac ttcaatgtat ttcacactat catactcaaa agaaaaatag
      961 acatgtaaaa gcattttgtt gtttacatct acataataat cctttgactc tacttgagac
     1021 cttcctatac ggaatacagc tttactgtct gatatctcta taccagcatc cttattatta
     1081 atctgccaat ctccaagtag ctcttctggc aagctagtag tttcttgtga acactttacc
     1141 acttcactgg tagaggtcga aacattcacc tccgcactgt tagaagaatt ttccaaggaa
     1201 tcaggagcct tagcacaacc aacaagtctt tatatattgt gcagccgata ttttcttcat
     1261 tcagtacacc cttgaaatat gcagtgttag ataaagcagt tcgtcctgat ttgctgaggc
     1321 acccatctct tcttgaacgt actccttgat tttcaaggcc gcccgatact ctttcggaaa
     1381 acgctgcttc actgaagcca gcaaaaaatc atccttctcc ggcaactctt ccttcttaat
     1441 cattcgggca ataaaaaact gtaaatgcgt cacaaatctt aagtaaagca tcgaatcttt
     1501 atcaaccttt ttttcgggaa acagatatcc tagcatagcc aagatatcct ttagtgctct
     1561 taccgagagc atggtttctt ccatatttgc ttgttcactt tgggcattta caatgtgaaa
     1621 cgcaatgttt ccagcctcct cttctggcaa ctgaacattc atttttttgt tgatcaattg
     1681 gatagcatag cagccgatct caaattcttt tgggtaatac ttttgaattt caatcagcag
     1741 gcgattttga aaaacaatgc ctttttgact gcgttcaatg gcatgcttca agtgatcaac
     1801 caaggtaaaa aatagacttt tattcagctt aaagtcaaac ttggcgttgg cataatcgat
     1861 aattcgcttg acgcaaataa ttagatcatc cggagcagcc tcaaacagtt taatgtaatc
     1921 cttttttaga gcatcagaaa gtgaatctaa tttaaatact ttttggattt tgtcagccgg
     1981 aaccaaatcg cctttcttta gttgaaatcc aatccctttc cccatcaaaa ttacttcttt
     2041 gccttgctta tctaagcaaa gaattaaact gttgtttaac gttttaatta ctctcattag
     2101 attcaccgtt ctctctaagc taagccattt cttgcgatta ctttttgata ccaaaagaag
     2161 ctttcttttt ttattcgttt taattcttta agattctcat cggttcggtc aacatatata
     2221 aaaccataac gcttattgaa gccttgatgg gtactaacaa catcaatcgc tgaccaaggt
     2281 aaataaccaa aaacatctac accatctgtt aaagctgctt gaatagccag aatatgtttg
     2341 gctagataat caattcgatc ctggtcatta attcgtctat cttcctcaac tatgtcgggt
     2401 gccccatagc cattttcagt aattaaaata ggtaaatcat agcgatcgta caatttcctc
     2461 aatgttaatc gtagtccagt agggtcaact acccagccat atggcgtttt atctacataa
     2521 ggattttcag ctgcccggta gacaccttct tctcccaaca tgatctgttg atcccccgct
     2581 ctagcggaaa catctgatcc gtcatttttg ctggcagcga tggtggcagt tgaataataa
     2641 ttaatcgcaa taaaatcggg tttaccggag gataaaattg cctgatcctc tgcctcaatt
     2701 ttaggatata atccacgatc attcaaatag ttttcaaaca aataattgta ttttccccga
     2761 actgctacat ctaagaaaga ccagcctctc aatgtttccc aattatgggc tgcaattgca
     2821 tcttctggac tggaggtagc ttgatacata gccgttaaat tgattgctgg accgattttt
     2881 cctttaagat tcaactgatg atacaagtga attactctag cttgagctaa taaaacatgg
     2941 tgattaattt gaaagagttc tttttttgaa ggcagctctc cacttggtgg caaaccaatc
     3001 gctccaggat gaaggatcat cgtgttttgc tcattaattg tcagccaata gttcaccttg
     3061 tcaccatact cagtaagcaa tacctgtaca tagtccacaa aataatcgat agttgctcga
     3121 tttaaccaac cgccctgctc ctctagaaaa taaggtaaat cgaaatggta catggtgaca
     3181 atcggttcga ttccctgatt caggcattca tcaattactt gatgataaaa agaaattccc
     3241 ttttgattgg ttttattatt ttcaatatcc ataattcgcg tccaagcgat ggaaaagcga
     3301 aaagctttta agcctaattc tttaaacagc tgtatatctt ctttatagtg atggtaaaaa
     3361 tccgaggcaa cttcaaaatt agctacccct tctgtatgct gatacttgtc aataatagac
     3421 ggttgtttcc catcctcctg ataggcaccc tctgcctggt aggcactaat ggaggcaccc
     3481 cataaaaact tctctggaaa tgctgaaagc tgtttgtatt gcacaaaaat cctctcctta
     3541 actaatttgc atcaatggtt cgtgttcctt taagcttccg cttgcttcac taattcctgc
     3601 gtaattttca ctgttggtca ctaccacaat cactgtatca tcgtagccag cgtttctaat
     3661 ggcagcgaaa tcggcgttga ttaataattg tcctgctgac acctgatctc ctgcttttac
     3721 aaatacttca aagggttccc cttttaaatt cacggtgtcg atccccacat ggatcaataa
     3781 ttcaatacct tctttagaac gtagaccaat cgcatgttta gattctaaaa tagtaatgac
     3841 ttctccctca acaggtgaat aaatgctgtt ttcttctgga tgaatggcta cgccctttcc
     3901 caatagtccc ttagaaaaga cagcatcgtt cacttcagaa ataggacaca cggttccttt
     3961 taccggattt tcaacaatga ctgcttcggc tattgccgtt tgctctgatt catcagaagt
     4021 atcgtcaaat ccaattagat aagcaatgat aaaggttgca acaaaggcga ttacacacgt
     4081 aataattgcg tgaacaatat tcattggatt ttctccaata aaggctggta aggctgctaa
     4141 gccaggggaa acaaaggcat atctgactaa accgctcaag ccagcataca aaccagcaat
     4201 tgcaccacca atcatggaag caactaatgg ttttttcagc ttcaaggtta ctccatagag
     4261 tgaaggctca gtaattccca ttaaagcagt gatcccagtg gacacagcta gctgcttcaa
     4321 ctctttgttt ttagttttcg cggctacagc aaaggtagca cctgattgag caatattaga
     4381 agccagcatt cctggcccat tgatcatttc agaacccgta ctgctcaatt gcatagtagc
     4441 gattggcgtc attgcccaag ctgtacccgt aaccaccaaa aatggctgca aaccacccat
     4501 caaaaatgga attaaccaag ctgctttgcc gttaatccat tcagccccca ttgctataag
     4561 atcgttcagc aacgttccaa acggtccaat gacaaccaac gctaaaggcc ccataattaa
     4621 aaataccagc atcggcttag taaagaattt aataaacgaa ggcgaaattc gatccgcaaa
     4681 gggctctaca taggacatga tccaaacagt aaagataatc ggtaaaactg atcccgcata
     4741 atctgcagct aataccggaa ttcctagaaa agaaacggca tcgccagtag aaaaaagctg
     4801 tgaaatattt ggatgaagca aaatgccagc aacagtaatc gccaaaattg ggttgacatc
     4861 aaactttatg gctgccccat aggctaatag tactggcata aaataaaagg ctgcatcagc
     4921 aatagtattc aaaatgatgt aagtttgact actgtctgat agtacaccag tcaaagtcaa
     4981 gacggccaaa atggctttta ccatcccggc acctgttata gctggaatca tcggtgtaaa
     5041 ggtggtagaa atgacactaa tcaatcttga aataatactt tcttgattgt ctgaagaggg
     5101 gcccttttta attgaagttc ccacttcctt ttgaatttcc ttgaaggctg tttggacttc
     5161 attgccaatc accacttggt attgtccacc gctatccacc acactaatta caccaagtgt
     5221 tttttctaag gctttcgtat cggctttcct tttatctttg agttcaaaac gcagtctggt
     5281 ggcacaatgg gttaagctga ccacattttc ttttccccca acctgttgca atacatttct
     5341 tcctacttct ctgtaatcca ttattctctc tccttaaagc agtaaaaaag accaagtcaa
     5401 accacgcccc aatcaacatt gaagtgttag tttgtcttgg tcttgcctgc ataatcagta
     5461 acatgccaat aacgttattt tgctgtaatc caataatagc acattatgat agcgcaatca
     5521 acagggaatt taatttttta tcttttcgtt tgaccgtata gggtaatata acagttagat
     5581 cacatcaact agggtatatc tgcgtatact tactgctaaa ataagtatat ctaatattaa
     5641 ctataccagt aaaaccattc ctacataacc agcgggaaat tcccaaaatc gtgaaagcat
     5701 caaaaatgca tataacggat cacacaactt caacaattct gctcttggga aaaattgatg
     5761 aatttggtga gtaaaggatg gaataaacag cattccaaca atataggcaa caatagtgat
     5821 acttgttttt ttggtaaaaa tcagtaagca atagccaatt tcaatcattc cgagatataa
     5881 aggtatcgat agaatgattt gggctagtat tcttaaaaat taactagcaa atgtttaaac
     5941 tgatttattt ctaatctaca tgtagtaagt atttcaattc caccaattat caataaaaat
     6001 accaaacata tccataataa ttttttcttt ttcatttctt ttatattgtt ataacagtga
     6061 ataacataaa aattcccgac aatcattgta atattttttt gtcacctgta ttagtattta
     6121 atttatagga aactataaaa actccctagt gaatttacac ctttgggagt tcctgtttgg
     6181 acggtatgca taatacattt aaatcaattt aacattaaca cctcagcata tggtatctcc
     6241 acatatatac ggtctttctt tggatagtac tcaatattta tgttgatagc acttccattg
     6301 tctaatatag ctttagcccg gtatgtgtta tctccatttt catgagtaat cgtcacatca
     6361 tcacatgatc gatttaagtt gtactgttct tgtaaaatct gtgttaccaa tggttttgat
     6421 gacttctcta atccagatgt acctgtcatt tttgaaaaaa taatacctat aatagccaag
     6481 acaacaatac ctataatgac aaacttcttg ttcatattta ctttattcaa ctttttatct
     6541 cctatctcca aaataatttg ctcttatccc tcttatcagt acattgacat ccctaaaagt
     6601 acaccaaaga ttgctccagc tacccctgct atcatcaaaa tcgtacccat tttctcatct
     6661 ttttctcgat ataagtagcc cataataaca gcaacagcac caaatacaac aggaataaat
     6721 gctaacgata ctaccataga aatccaacca ataatcaaat acagcttccc tgtctcttgt
     6781 ccatgtacga tttgttcctt catttcatta attgtctgct cagtactttt ctttactgtg
     6841 tctgttccgg ttgtctcatc tgtttcttgt agtgctccac aaaactggca atacttactt
     6901 tcatctgtta tttctttgcc acattttcta caatacatgt cttctcctcc taaaatcatt
     6961 caattgctta ttagttaact gtttacttta ttaaatacat cagaaaacag ctcattcatt
     7021 ggtacactta atatctcaga aagcatcttc atttgatagg gtttaggaat cgttctccct
     7081 gtttgccacg aacttacaca ttgctgtgta acattcattt tttttgctaa ctccgtttga
     7141 gaaagtcctt tttcttttct gtatcggctg agattactta cttttttcat gattctcccc
     7201 tcctgtttta ttatatacaa caatatgatg tatttcaact cataaaatat aaaaaacttc
     7261 aaataccatt acaaatacaa caacttgttg tactatactg agatgggggt gtattaatga
     7321 aacagaaaca atatgcaaag gtcattagag aaaagcgaaa agctctcggg tggacacaaa
     7381 aagaattagc tgaaaaaatc ttctctacac aacaggcagt agcaagatgg gaaaattctg
     7441 taacagagcc taacctcgat agcttaacag ctctttctag agccctagga acccctgtaa
     7501 gccattttct agacaatgtt gtcgtagatt atgaggaaga gtttttggct ctctatcgtt
     7561 ctcttagcac tgaagatgca gttcgaacaa ttgactatat gaagctatta aaaaggcaag
     7621 aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg aactttaacg
     7681 gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta gtttaataca
     7741 attgggtctt ttctcttcct tactctccgt ccatcatctc ttgttacact tgtaaattga
     7801 tacagaggaa ctatcccagc tcctgataat atcaattctt ttattgtttc atctatccct
     7861 gcccctttcg ctaaatatat taaaaaacta ctacttgctt tcacattgta tagtttctta
     7921 atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc tctgtttttt
     7981 attaaagcat ttttcacgct gttatcccca acattacgta ggcgttcaca aacagtgtta
     8041 ataagttctt tctctgtcat tagatatgtc ccttgccacg ataagccctg taaaacttca
     8101 tgtgcttcat ctaaaatcaa ttgtttttcc ttgctgagtt ctgatcggga attttgagga
     8161 aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata agaaaaatat
     8221 tgatatgtaa aatgaatttt ttccagaagt ggaaggatat cttcattcaa tttttgaatc
     8281 acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg attaaagttt
     8341 cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa aggaacatct
     8401 tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa cttattacat
     8461 ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa ttgttccccc
     8521 gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc agaaagcaag
     8581 acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa aagtttgtct
     8641 ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt tcgaaaatgt
     8701 gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg actgtctttc
     8761 tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt actggaatga
     8821 caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg attatgatcg
     8881 ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat atcaatggtt
     8941 aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag gcagtcttta
     9001 aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa atcggtttca
     9061 ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca tgtcatggta
     9121 aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat tatcactgca
     9181 tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc atcacacccg
     9241 gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc agtaattgct
     9301 tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg aatacttttt
     9361 tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc tacgcttaag
     9421 tatctactat catctttact caaatctact accttcccct tctcatcttt aacagtgtta
     9481 tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt gcctttattc
     9541 tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa ctcatctctt
     9601 gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc tttttttgta
     9661 actggcttct ctaataattt ttcttgatct acataaattg gaatattaat cattgatgtt
     9721 tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca gataataaaa
     9781 acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt tataccgttc
     9841 catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt ttataacagg
     9901 taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta gatattcact
     9961 tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt ctagcatagg
    10021 ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa caatgctatc
    10081 aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa attttctaag
    10141 aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg attaatcaaa
    10201 caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa aggggtgtca
    10261 acatctttgt aaaatttatt tacaacaccc cttttttgag gtatactaat tttaggaggt
    10321 gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa gatactcgga
    10381 acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt tggtcttatg
    10441 attaacaacg gtcaattaat tgacaaaaaa actatctacg aatgggagaa gggaacctat
    10501 ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag tatagaggag
    10561 ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag agatagcatt
    10621 gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg tcaacagttc
    10681 ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt agaaccagag
    10741 atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa aattagtctt
    10801 ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaaa atttgacaat
    10861 aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt agatgaatgg
    10921 ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga aattactgat
    10981 aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac taagaaaaaa
    11041 atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga ggagatgatt
    11101 taatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg atgtataaag
    11161 gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga agaggtttta
    11221 gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata ttatacggtg
    11281 ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg ataactcatc
    11341 agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca aataatcaaa
    11401 tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat tgtcaaaaag
    11461 tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa cagactgcac
    11521 aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg gaaaaaacac
    11581 ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta
    11641 ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc ttcttcagat
    11701 tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg aaagacatag
    11761 acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa tttggtaaag
    11821 ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta gatactgaga
    11881 ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta aaacttggat
    11941 acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact ctatacattc
    12001 caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg cctcgtatta
    12061 cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga gaatctgtca
    12121 aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac atctatgcac
    12181 atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg atggctcatc
    12241 agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca aaaaagctag
    12301 aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac tgccttccat
    12361 ctcataactg atcaatcgat tgagttcgac tgcatattcc attggaagtt ctttggtgaa
    12421 tggttcaaca aagcccatga cgatcatttc agttgcttca gattcggtca ggcctcggct
    12481 catcagatag tagagttgtt cttctgagat ttttgagact ttggcttcgt gttctaaaga
    12541 gacttggctg ttgtggatct cattgaatgg gatcgtatcg gatttcgacc attcgtccat
    12601 gatgatcgtg tcacattcga tatgagagat cgaacccgca ctgtttttgg caaaggttac
    12661 ttgtcctcga tagtttactt ctccgccgtc ttttgaaatt gatttagaga cgatagagct
    12721 agatgtattt ggtgcattgt ggatcatttt tgcacccgta tcttggattt ggtttgctcc
    12781 tgcaaaggcg atggagagca tggtgcctcg tgcacctttt ccatctaagt aaacacttgg
    12841 gtatttcatc gttgtttttg cacctaggtt tccgtcgatc cattcaacgg ttgcgccttc
    12901 gtatgctttt gctcgtttgg ttactaagtt gtacacattg tctgaccagt tttggatcgt
    12961 tgtgtaacgg caatacgcat cttttcttgt aaagatttct acgatggcgg catgcaggct
    13021 gttacttgaa taagtaggtg ccgtacaacc ttcgacatag tggatgcttg ctccttcgtc
    13081 aacaatgatc agcgtacgtt cgaattgccc cgtgttttcc gcattgatcc ggaagtacgt
    13141 ttgtaatgga acatcgactt tgacggcttt tggcacgtaa ataaaggtcc cacctgacca
    13201 tacggctgaa ttcagcgctg ctaatttgtt gtcggttggc ggtacaagtt ttgaaaagta
    13261 ctctttgaat aattcagggt attctttcaa agcagagtca gtgtcagtaa agacgattcc
    13321 taacttttcg aattcatctt tcatattatg gtacacgact tctgattcat attgtgcgga
    13381 agcaccggct agataagctc gttcagcttc tggtatacca atcttttcaa atgtatcttt
    13441 gattttttct ggtacgtctt cccagtcacg agcgggtttg tcacttggtt tttggtaata
    13501 tttgatggca tcgaaatcga tgtctgaaag gtcggggccc catgtttgca ttggcatttt
    13561 gttaaatgtt tctagagatt tcagacggaa ttcaagcatc cattctggtt cgcctttgat
    13621 tcttgacatc tcacggatca cttcttctgt caagccttct cctgtactga aaaccggttc
    13681 gacatcatcg tgaaacccga atttatattc ttctaattct ggtacgccca tgttctcact
    13741 cctcttcctt gtggtaatga tggaaagctg cggtaccatc gttttccatt gcttttccta
    13801 gtgctttcca ggcaagtgtc gcacatttga tccgagcggg aaatttagct acaccaccaa
    13861 gcatagcagc atcgcctaat tggtcgggat ctttgacttc ttttccttgg acaagctgta
    13921 gaaagtctac agttaaatgt tctgcttctt ctattgattt tccgatgacg gcgtctgtca
    13981 tcatcgaggc acttgccgta ctgatggagc agccactgcc gtcaaaggcg atatttttga
    14041 tgattccgtc ttttacatcg acttgcaatt cgatcacatc tccacatgtg gggttgttca
    14101 attcgatctt gcttgtcgag tcagtcaagc ttccacgatg atgcggatgg gaagaatgat
    14161 ccaagatcac ttgacggtat aaattgtcta atttagatag tcccatgttg gaaaaactcc
    14221 tttgttgcca tgattgcttc aactaaacgg tcggcgtctt gcgttgtgtt gtagaaataa
    14281 aagctcgcgc gagcagtcgc tgatacttca aggtacttca atagcggttg cgcacaatga
    14341 tgacctgctc tgacggctac cccttccatg tctaaagctg tcgcgacatc atggggatgt
    14401 aacccatcca aattgaatgc aacgactcct gtccgaaaat tcgggtcttg tggaccataa
    14461 acagtcaatc catcaattgc ttgtaatttt ggcaacacat aagcggctag ttcttcttca
    14521 tatcgatgga tattgtccat accgagtttt tgcaaataat cgattgcatg gcctaatgcg
    14581 atagcgccgg cgatatttgg tgtccctgct tcgaatttcc aaggtagttc tttccatgta
    14641 ctgtcgtata attcgacaaa atcgatcatt tcgccaccaa attcaacagg ttccatttct
    14701 tctaaccatt ttcgtttacc atataaaaca ccaatacctg ttggtccgca cattttgtgt
    14761 ccgctgaaag caaagaaatc acagtctagt gcttggacat caatggccat gtgcggggca
    14821 gattgagcac catctactac gatgaccccg ccgttttcat ggacaagttc agctaattct
    14881 tgaacaggat tgaagacacc taagacattg gatacatgag caatggagac gattttggtt
    14941 ttgtccgtaa tctttttcgc tgcatcctcc atatccagga agccttcttc tgttagttcg
    15001 atatatttca agacagcacc tgttcgtttt gccaactgct gccacgggat aacattagaa
    15061 tgatgttcca tgtaagagag cactatctcg tctcctgctt tgatatagcg gtcgccgaag
    15121 gttctggcta cccaattcaa gcttgtcgtc gtaccacgag taaacagcac ttctgctgtt
    15181 tcttttgcat ggatgaacgc ccgtactttt tctcttgctt gttcataatc atgggtcgcg
    15241 cgttctgcta acgtatggac accgcgatga acattggcgt tatcgtgttc gtaatagtac
    15301 gctaatgcct ggagtacttg ttttggcttt tgggtcgtcg ctgcattatc cagataaact
    15361 aaaggttcat cattgacgtc ttgaaataag ataggaaaat cattgcgtag ctttttagca
    15421 tcgatcatcc attcaacttc ccttcgatta cttcgactaa ttctttccga acttgttcta
    15481 cagggattgc tgttaagaca gaaccgagga atcctcgaat cactaagcgc tctgcttctt
    15541 ctttatgcaa tcctctactc atcagatagt acatttcttc tggatcaact cgtcctacac
    15601 tggcagcatg tccagcagtt acttcgtttt catcgatcaa aagaatagga ttcgcatcac
    15661 cacgtgcttt atcagaaagc atcaagacac gactttcttg ttgggcatct gcaccttttg
    15721 cccctttcaa gatgtggccg ataccattaa aggtcaatgt tcctttttct cggatcactc
    15781 cgtgttgcaa aatatgcccg atcgtatgag gggctttatt ggttactcgt gtatcgattc
    15841 cttggatttg tctgcccgag ctgattgcca cgattttgac ttctgcgtgt gcaccttctc
    15901 ctgctaaatc tgagtcaaag tcagcgatca catgaccgtc attcatgaca ccaattgccc
    15961 agtcgacaga agcatctcgt aaaatgtgcc cacggcgatt catataagaa gtaatatttt
    16021 ctcctaattg atctacggct gaatatttga tttttgaacc agctttagcg atgacttcca
    16081 cgatgatatt acctgatgat tttgctactt gttctttggt tgtctggaaa cgttccagat
    16141 aagaaaattc actatgttca tctgccacga tcaacacatg cttgaagaag tgctcatcgg
    16201 atgcgccatc ttgaatgaat aaggactcga gtggttcatc gatcacgaca tttttaggca
    16261 catacaggaa aataccgctg ttcataaaag ctacatggag tgcagtcaat tggtcttcgt
    16321 tcatgtttac tgcttttttc atataatatt cttggactag ttctggatat tcgatcattg
    16381 cagtgaacaa gtcggtaaaa atgactcctt tttctgctaa ttccacaggc agttgttcaa
    16441 aaatcgtcca tgaaccttgt tggacaagca caggattgtc tttcattgca tcaaatgccg
    16501 gaatcgtgcc tgtttcactg atggtttgtg tttctttcac atcaaataac gaccagcgat
    16561 ggaatttcac ccgatcgata tgcggcaatg gcagttcatc tgctttcgct agtgcgtctt
    16621 gacggaaggt tctcatccaa tcaggttcgc ctttacttaa agaaaaatca tttaccgcgt
    16681 caagataatc gagccagttt ttttctttca ttcttgctcc cccttacgct tcttcttctt
    16741 tatagtcgat tcctaattct ttactgatac cagcgtagcc ttctgcttcc agacgtttag
    16801 ctaggtcagc attccctgtc atgactactc ggccttccat catgatgtga acgacatcag
    16861 gtgtgatata gttcaataat cgttgatagt gggtgatgat caatgcaccg aaattgtctc
    16921 cgcgcatttc atttactcct ttagcaacga ctttcaaagc atcgatatcc agacctgaat
    16981 cgatttcgtc taaaattgca aatgtaggtt ctaacatcaa caattgcagg atctcattcc
    17041 gtttcttttc tccgccagaa aaaccttcgt tcagataacg ttcagccatt tcttctggca
    17101 tatttagtaa agccattttt tcatctaact ttttcaaaaa tgccataaca gaaattttgt
    17161 cgttttcatc acgtttcgca ttgatcgctg cacgcatgaa ttccgcattc gtgatacctg
    17221 gtatctcact tggatattgc atagctaaaa ataagccaag tcgcgcacgt tcatctactt
    17281 ccaagtctaa aacatttttc ccgtcaaaca aaatttcgcc ttctgttact tcatagtttg
    17341 gattgcccat gatagcggca gataaggtag attttcctgt gccgttaggt cccatgatgg
    17401 catggatttc tccggttttc attgttaggt ttacgccttt aaggatttct ttttcctcga
    17461 tcgacacatg tagatttttt atttctaaga cagacatgca ttataccccc aaattctctt
    17521 tcgttctcac tcattttaga ctaattgaga atgaagcgca atgaccttcc ttactaaaat
    17581 caaatatctt ttatttatta ttaaacaatt ttgatttcta aattagaata ggtataaaaa
    17641 agatgagaaa taagccatta cagacttatt tctcatcttt tttgttccat accttatttt
    17701 gcatcagcct ttactggttt aacagagccg ccccatttgt ctgtgatcca ttcttggact
    17761 tctttgcggt gaagcacttc aatcagtttt ttgattttgt cactgtcttc atcaccttta
    17821 cgagtcgcaa taatattcac gtaaggagag ttgtcctttt caagcaatac ggcatcttct
    17881 aatggattca accctgcgcc gtaagcgaag tttgcgttga ttgcaactag gtcaccttca
    17941 tcattttggt acgtagaagc taaaagtgat gggtcgatcg tatggttgaa cttcaagttc
    18001 ttagggtttt cttcaatatc atcgaatgtt gctgtttcca gatctactcc atctttgact
    18061 ttgactaagc cagcatcttg tagaattgtg atgatgcgtc cccagtctga ttcagaattt
    18121 gaagtaatga ttgttgcacc atcttttaat tcggaaacat ctttgacttt tttagagtac
    18181 aagcccattg gttcgatgtg gacagcaccg gcattgacaa agtcatatcc tttttctttg
    18241 atttctttgt tcaaataagg aatatgctgg aagtaattgg catcaatgtc gccttcttcc
    18301 aaagattggt tcggtaatac atagtcatcg aatttgacga tttcaagatc gattccttca
    18361 tctttcaata atggtttcac atgttccaaa atctctgcgt gcggtgtcgg tgaagcgccg
    18421 actttcaatg tcgtgtcgtc tgctttattt gctgaatctt tgctgtcgct gttgttaccg
    18481 catgctgcta atcctacagt cagcaaaagc gctgctgcaa atccaaaaat tttctttttc
    18541 atatcctaca tccccctata tcctattatc ttttgtctgt tttcttagtt aagaaatcac
    18601 cgattccttg aacgataaat acgattagta aaataattaa agtcgcaacg aatgtcactg
    18661 ttagattacc acgctggtag ccttcctgcc aagcaagacc tccaagtcca cctgcgccga
    18721 tcgcgccggc catcgcagta aatccgatca tcgagatcgt tgtaactgtt aatccagaaa
    18781 tcaatgcggg aatactttca ggtatcaaga ccttccagat gatttcccag taacttgctc
    18841 ccattgcatc tgctgcttcc agtacaccag ggctgacttc acgaaaagca atctctacta
    18901 gacgggcata aaatggtgct gcggataaaa caagagctgg aatggctgct tgcgcaccta
    18961 gcatagtgcc tacgattgct ttagtaaatg gaatgatcaa aaccatcagg atcataaatg
    19021 gcgttgaccg gaaaatatta ctgatgatcg atacgattcc ataaatgatt cgagcatacg
    19081 gtttgttttt gcgtccaata gagtaaagga tcaatcccaa aactaatccg atgatcgtca
    19141 ctaagaccat tgagattaat gtcatatata gtgtatcaag gatagcctga ttcatcgttt
    19201 ccgggttgat ttgtttaaaa tctagatatg tttctgaaaa gcttttatcc acgatggatc
    19261 acctcaattc ctactccttg ttctttgatg aacacttctg cttgatctaa ttctttggtt
    19321 tcaccagtca tcaagacagt caatgaacca aaggaacctt ctttcacttg tttgatcttt
    19381 ccatacacaa cgttgacatc gatgttatag cgtcgaatcg cttgggaaat gactggctcg
    19441 ttggcatttc tttcgctaaa ggtcaaagtt gccaaacggc cgcttggatt ttctttgatg
    19501 agttcagcta atagttctgt cgaatcagct tctggttcga tgtcttgttg tacaaagcgt
    19561 ttggttactt cttttttcgg ttgtctgaag acagtcaata cgtctccttc ttctacgact
    19621 ttccccaact ccatcacagc gactttgttg cagattttgc ggatcacatt catttcatgt
    19681 gtgatcaaga cgatggtcaa attcaatttc ttattgatcg ctaacaataa gtctaatact
    19741 tcttctgttg tttgcgggtc caatgcgcta gtagcctcat cacaaagcag gatctcagga
    19801 tcatttgcca atgctcgtgc gataccgacg cgttgtttct gtccgccgga aagttgtgaa
    19861 ggatatgctt gtccgcgtcc ttctaacccg actaaacgca acagttcttc tgcccgttct
    19921 ttacgtttgc tctttggtac gccagctaat tccagcggca gctggatatt ttcaagtaca
    19981 gttcttgacc ataacaggtt gaaatgctgg aagatcatac cgattttctt tcggaatgta
    20041 cgtaattctt tgttttttaa ttttgtaata tcttgatcgt ttacgatcac ttcgccgctc
    20101 gtgggaagct caagtccatt caggagacgg actaatgtac tttttcctgc gccagaatag
    20161 ccgacgattc cataaatatc ccctttttca acgtttagtg aaacgtcatt gacggcttgg
    20221 atgtttccgt ttttcccatt gaatgtcttt ttgacattcg ttagttggat taatgacatg
    20281 ccattcacat ctttctctta tgatttttca aacaaaaaag ccttcgccct cttgccttaa
    20341 gcaaaaggac gaaagcttga acttccgtgt taccaccttg atttaccgtt acttcgcaat
    20401 aacagcctct tccagtactt ggacttagct tctttccgta tactgtcgcg ctgtaacggg
    20461 cactcccgta gtaagtttgc ttacgctcgc ttactctgct ctaagaccat cttccatctc
    20521 attttcgtta cccattttca gcaaacaggg ctctcttcaa acgtccgtga catgtactct
    20581 tcttttcaaa gctttctcgt ttcatatagg attgcttatg attatagcaa ctggaagaat
    20641 cagtgtcaac ttttttcaag tgattttctg attttataat tcgtcaaatt cttttggatc
    20701 aacatctttg aaacttaaga tccacgcatt catttcatct gaatcattca agatattgat
    20761 atcttcaccg attttatcat taacagaaga aactgtaccc gttagcgggc tggcaaattc
    20821 attgaccgct ttttccgctt caacttcgat cagaggttca tcttttttta gcgattgtcc
    20881 tgcttttggc agattggcaa acgtgatgtc tccaagttct tcttgcgctt catttgtcaa
    20941 accaacacag tattcttttc cattaaataa gatccaaagg ttgtcctttt tcttcaaatg
    21001 cttgcctgcc attttatatc agttctcctt cataaatttt ttcattgaat ccaattgcaa
    21061 caagctggtc ctctcgtaag atcagtggtc gcttgatcag cataccatct gtcgataaga
    21121 cttggctcgc ttttgaagcc gtaaactcgc ctacttgatc tttcagtcca agttcccgat
    21181 atttcatccc gcttgtgttg aacaggcgac ggattggtac ttgactcgtt tctagccaag
    21241 tctcaatcgt tgaggccttc ggtacttctt tgatcatatc aacggtttga taactaacgc
    21301 catgctggtc taaccacgct ttggcttttt tgcatgttga acatttcggg taccagtaaa
    21361 aggtgtacaa ttttcttacc tccttgaaga tcttgcaggt gcttcatttt ggtatctcat
    21421 cgataatatc aacccgatac cgatcatgtt ccctaaaatc gaggatcctc cctgactaat
    21481 gaaaggcaat gggatccctg ttaaaggaag taatccgata tttgctccaa tattctcaaa
    21541 gacatggaac agcagcatca tgatcaaacc gcttgcgata taggcgtaaa attcattatt
    21601 cgtatcgaaa cacacccgaa tcatacgata gattaaaata aaatacaaga aaatgacgaa
    21661 tgcaccaccg ataaagccga agttttcacc gataacggta aaaatcatat cggactcacg
    21721 aacaggcaca tagatattgc ttacgttgaa tcctttacca aacaagccac cggtaccaat
    21781 tgccaaaagt ccttgggccg gctgataact cattcctgac gtatcatgga aagggtccag
    21841 ccatgaatca atacgtgcaa actgataaga tttgaatcca actttataaa gcaagtctct
    21901 tccgccttca gtcgtaacca agaagatcgt tcctgctcca acgactactg ccaagattac
    21961 tacaggtgcg atgatgcgcc aagagattcc agacatcagg aaaatcccgc caaagatcgc
    22021 aagaaagaca agcatcgttc caaagtcatc ttgggccatg atcaatgcta aaaccggtat
    22081 tgtcacgatc aagagcttcc caatcagcca aaagtccgat ttcatcgtcc gttcttttac
    22141 ttgcgtatta tgtttagtta cgatcagtgc catcatcaag atgtacgcaa tcttcatcaa
    22201 ctctgctggc tggaaagtaa acgcaccgaa tcgaaaccag tttttagacc ctgttgaagc
    22261 agctaaattc tgatcataga atttgagcaa gagtcccatt accactaacc ccaaaccata
    22321 aaggtaagga gtcagtttcc agatccattt ggaattcaaa tgcatgatga caacgattgc
    22381 tgccgctcca actaaatacc agacaacttg catccctacg cctttcaaga ctgctggatt
    22441 gttcggatca tggctaagtg ctacatataa tgagagcaga ccaatcaaac ataacagaaa
    22501 gactggtaaa atcacgccat aatcaatacg attatcgttc gaagctttta attttttttc
    22561 catgtttcat cccttttctt aaaaaccata ctgctgatta ttatagcggt ccagttagat
    22621 aatggaaagt ctttccatag acatttggga aaaattaact ttccgtttgt gcagttgctt
    22681 gagcaaatgc agtacaaaag cctactgctt atctttagtg tatcatagaa ctttttttga
    22741 tacatgcaat tatctaccag ctgagattct cctcgctgtg agaagtttct gtgctttcat
    22801 tgtatcgaag gatctcttcg gcaatctcta gcagctgatc agaaaccaga ttggttcgtt
    22861 ggatgaaata aaaatatcga tggaaattca acggagtcat tggcacattt tctggaacag
    22921 agcgcttaga caaaatcgag cgaccgattc cttccttcag caacgcaaca attacatcgt
    22981 tacttttcac gatcatcttc ttatcttgga tattcatctc ttcaaaataa cgctgcgtgt
    23041 aatgaaaaac accagagttt gcttctctta caagccacaa tttgcttttt gggtccccag
    23101 cgatgactag ctggtcttcc atcaaagagt atctttgtac gccggaagtc tctaatggct
    23161 tttcgataaa accaaaatcg gattcatgtt tcgctacttg ttctaatgat tcatgagaat
    23221 tcgtgatttc gatctcaaaa tgcactttag gaaacctttt gatcagatgt ttcatcaact
    23281 gaggcaaaag atagacagca aaggtgtggg aagcaacgat ccgacatact tcttcttcat
    23341 tttgttcatt gagcaatttt tgctgagttt cttcccattc ttctaacaaa accagtgtcc
    23401 gttcatataa taagtcggct tgcttagttg gaagcatctc ttttcttccg ttacgttgaa
    23461 aaagatcgat attcaattcc cgctccaact gtttgatttg attcgagact gctggttgag
    23521 ataaaaacag tacttctgct gctctggaaa aatttcttgt ttcatatact gctcgaaaag
    23581 tcgttaataa cttaaacatg gcaccacttc cataactttt tatgataatt actataaaaa
    23641 ctatttgatt taaatataca aaaaaccatc tataatagaa atgtacaaca aaacagttgc
    23701 tgatccaact agtaacttag atgaagacaa tcagctgtct gcttgctcgt aatcatacat
    23761 tcctattcat tttacaatgt attcgttcat cttggtacta aaatctatta aataaaaagg
    23821 agaatattat atatgaagaa ctttttgaaa attattccga tcccgatctg cgggttgatc
    23881 cttgggttag cttcccttgg aaatctgttt aaagactatc accatgttgc tttaggcaat
    23941 ctaactggcg gtatctctat gattcttatg atattcattt ttgttaaatt gtttatctta
    24001 ttcgaacaca cgaaacaaac gttgcatgat ccaatcatcg cctccgtttc accaactttt
    24061 acgatgtcac tcatggttat ttgtacttat tttgtttcat atgaaagtat tgcaccaatt
    24121 gtcaaattca tttggctgtg cgctgttatc ttccaagtga tccttgtctt ttatttcaat
    24181 tatcaccatg ttatcaaagc tgatctttca attgaagcaa tttatccaag ctggtttatc
    24241 atttacgttg gatttggggt catcactgta actgccggga acttcttccc acttattgga
    24301 aaaattttct tttggatttc attgatctgc tatgcgttct tattgccgat catcatcaaa
    24361 cgtgtctttt tcgtaagaaa tatggctcat ccgacacgtc cgctgattac gatcgttgca
    24421 gcacctggtt ctctttgcct aacaggttat cttaaaaact ttgctgaacc taatttttat
    24481 cttgtcgtag cattgttcgc tttatctcaa gtattgtatt tcgttgtgct gtctatgctt
    24541 ccacgtttgt tcaagcttcg tttttatcca agctatgcag cctttacatt cccgcttgtt
    24601 atctctgcaa cagctatatt tactactgtt cattacttca acagtttagg tatctatagt
    24661 agcgtattgg atttcttaaa agttgcggaa tgtctgtttg caagtgtagt tgtcttctac
    24721 gttcttgctc attacgtacg tttcttaatt aaagaacata gcaaacatgg aaaatttgtt
    24781 tctaaaaaaa tcgcttaata atcgattcaa taggttgtga aaaagctgtt ctgcatcaag
    24841 taataagaac ggccaaacaa aaacagctgt tcatgttttt gtactggttg ggcttattac
    24901 cgcagatgca agcttttgaa caccgtttac caagaggtcg taaaagaagt ggtcagcttc
    24961 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    25021 ttccgtagaa gctacttctg gaacaccgtt tattcgaaac aagaggtcgt acaagacttt
    25081 tgtcacgacc tcttgttctt ttgtcaaaat actggtttag tgctactatg ttcgagtatg
    25141 cttttagtat ttattgacga aaggaaccga tcaaatgaaa tcattgtcac atccaataaa
    25201 atctgtaaag gattcagcgg tccgcgaaat cttacccggt ttactgcttt cttttgccat
    25261 cgctgccttc agcaggcttc ttgctattta tctgccacag ttaggtggag cgactattgc
    25321 tattttattg ggtattttat taggaaatac tttttttaaa cagcctgttc tagcaatcgg
    25381 cacaaaattt gctgaaagca aactattaga attctctgtt tttttactag gtgcaactgt
    25441 cacagctcaa acgattgctc aaattggtgt aaaaggaatt gtttttgtgt tactgcaaat
    25501 gtcagtcact atcatcggtg cttacttgat tggtaaaaaa ctgcaattca gcgatacttc
    25561 ttctttgatg atggctggag gaaacgctgt ttgtggttct tctgctattg cttcaatcgc
    25621 cccttccatc catgcgaaag aggaagaaaa aggacagatc atcacactag tcaatctact
    25681 gggtactgtg atgatgttga ctctcccttt tcttggatta cagttgtttg gtggaaatct
    25741 cattgaaaaa agtgcacttt tgggtggtac actccaatct gttggtcaag tagtcgctgg
    25801 ggcaagctta cttgacaatt ctgttgtcga attcgccatg ctatttaaga ttctacgtat
    25861 tatgatgctc gttgttgttg tctatctgtt tgaaaaattc atgcacaaga aacaaccagc
    25921 tctgtttgaa acgccaactg atacgcagaa aaaacgaaaa ttaccgcttc cttggtatgt
    25981 attagggttt gtgatctttt gtttggtcaa cagcttgttt tctttacctg tagttattga
    26041 ccaatcggca cattttatca gcagctggtt tgagatcact gctttagcgg caattggtct
    26101 gcgtttagac tttgcaaaat tcatgaaaga aggtacacgc ttcttatctt atggattagc
    26161 tgtcgggctt ctccaaacag tgatggcttt actatttatt tggctgatca aagtttagaa
    26221 tgaacatgaa aaaacagttg tgattctcgc atggaacaat gtccgaatca caactgtttt
    26281 taattttgca gatatccttc taacaacaac aattggcgtt tgatttcaac cccatctatt
    26341 gcgtttccca tatatcctgt cagttttcca ttttgcccga tcactcgatg acaaggaata
    26401 acgattggca atggattggt tcgatttgcc tgcccaatcg ctcgcacagc tttcggacta
    26461 tcaacagcaa cggctacttc tttatacgtt cggatttctc cataaggaat ggtcgacaaa
    26521 gctttccaga cattttgctg aaaaggcgta cctctcttta tagataaagg aacatcaaat
    26581 tcttttcttt ttcccgcaaa atattcttca aactgttctt gtgtgcgcgt taaaattcgc
    26641 tgattcgctg attcgctttc tgaaattggc tgaaaagata cagcagtcaa tccggattca
    26701 tctgcatcta accataaaaa gccgataggt gtgtgtattt tcattttgtt tgctcctagt
    26761 caatagactg gaacttcctc ctttttctct atccaaaact acttgccaac taataaaaat
    26821 tgttcttaaa agtttgataa accgaggaaa catggtaaac taaatacgtt agtttacaaa
    26881 aagaatacct gttcttttac gtaaaatcaa attttaaagg tgttatttaa ggtttattaa
    26941 attttgaatg taaaccatat ataaacatac gattgaaagg atagaaacga tgaaagtatc
    27001 aattgttgtc ccatgtttta atgaagaaaa aactgttcct cttttttttg atgaggttga
    27061 aaaattcaaa ggtgcccatg agttcgaata tttgtttatt gacgacggat caaaagacgg
    27121 tacgttagca gcaatcaaga agctagcctc agcttatcct tatgtccgct ttgtctcatt
    27181 ttccagaaat tttgggaaag aagcggctct gtacgctgga ttgcaagctt ctacaggaga
    27241 tttagtgacc gtgatggatg ttgatctgca agatccgccc gaactgcttc ctgagatgat
    27301 caaccggatc actacttctg atatcgattg tgtcggcacc cggcgttcga ctcgtgaagg
    27361 cgagcctgcg atacgcagtt tctttgcgaa aaaattctac cagttgatca ataaaatcag
    27421 cgatacagaa atggtcgatg gcgctcgtga tttccgcttg atgactcgtc aaatggtgga
    27481 tgccgtccta gaattaacag aatacaaccg attttcaaaa ggattattta gctgggtcgg
    27541 atttaaaaca gaatatattt cttatgaaaa ccgcgaacgt gtggctggcg aaacatcttg
    27601 gtccttctgg aaattattca actactcgat tgatggtatc gtgaacttct cggatgcacc
    27661 gttaacgatt gcttcattta tcggcgcttt gtcttgtgtc ggttctggta ttgcaatttt
    27721 gttcattatt ttgcgggcat tgttgtttgg tgatcctaca tcaggatggc cttccatggt
    27781 ctcgatcttc ctgttcattg gtggaatcca attgttatgt cttggaatca tcgggaaata
    27841 tattgggaaa atcttcttag aaacgaaaca gcgtcctgtt tatatcataa gagaaacaga
    27901 aaaagtgaag ccaaatgaat tgaagcctca ccaataatat acgtcaaatg aagtaaacat
    27961 ttttttaaat gaaaaaggac tgtgacaaaa gtcttgtcac agtccttttt tccgaataaa
    28021 tggtgttcca gaagtagctt cttcggaaat aagccgaaat ttcacaaaaa tttgagaagc
    28081 aattttcgta aattccttct tatttcttga agctgaccac ttctatcaca acctctttca
    28141 tatcttatta cttcttcatt gctgctttta gtgctgctgc taatgggctt tcttctggtt
    28201 cttctgcttg tgcatatttt ttcagcaagc gtcgttcttc atgtttggtc attttcttct
    28261 tctgctcttt tttatcaagc aatttttctg tcgttccatc gtatttacaa cggaaataag
    28321 caccgttttt accttcgatg atttccattt tccggtgaca ttgcgggcat cgatgattag
    28381 agacttttgg atcttttctt ctgcggtagt tgcaatctga attggtgcag acatagattt
    28441 tgccgtcacg tgtgtttttc tcgcgcagat tggccccaca ttctgggcat ttcttttggg
    28501 taatcgagaa atcttgatat tcttgtttgc ttgatttgat ctcattcacc aagcgttttg
    28561 tttctttttc gattccattc aagaaggttc ctgcttgttt ctttcctgca gcaatagctt
    28621 caaggtcttt ttcccagctt tctgtcaaat ctggtgttac taaagaggga tttaccaata
    28681 ctaataattg cttaccttta ggagatactt gcagtccttg aggggtacgt tccatcagtt
    28741 cagatttgat tagtttctcg ataatctctg cacgcgtagc tggggttccc agactgtatt
    28801 tttccatttg accaagcaag cttgcttctg acaatggttt aggcggtgca gatagttctt
    28861 ttttgatggc gaaatcggga ttgattctca ttcctttttc ccatttgact gtttccgtct
    28921 cagtttcagt ttcgcctttc catcctgctt ggacgacacg attttgccga aaaatgaatc
    28981 gttctttacc aaattcaacc gtaacttttg tttggctgac tttatggggt tgagcaaaca
    29041 gtcctaaaaa acgagagaca atcatttgat agatttttcg ctcttcatta cttaattttt
    29101 cataacgtgc tctgttttca gttggcaaaa gtgcatggtg atcggtcact ttactgtctt
    29161 ggaatacttt tgtctgttgg acgatggctc cgtttttcaa atactgcttg acctcaggag
    29221 caaagtcgct gactgcttgc aatcgttctt tcatcgtact tttcatgtca tttgtcagat
    29281 atttgctgtc tgtacgcgga taagtaacga tcttatgggt ctcatacaaa ctttgtacaa
    29341 gacttaatgt tttctttgca gaatagccat agcgttgatt ggcttctctt tggatctctg
    29401 ttaaatcata aggcaatggc gcagattccg tttttaattt ttcttggact tctgtgacag
    29461 ttcctttgct tgtactgatc aattgaacaa gtttttgtgc ttcttcttgt gtttttaaag
    29521 caaattgatt tttttgaagc attttggctt gttcttttcc agcttgcaac gtgatcgtga
    29581 aatacgtttg tggacggaac tgctcgattt tcttttcttg gtctctcact aatgctagtg
    29641 tcggtgtctg cacacggcct gctgataaat tatccgcgta cttcacagtt aaggctctgg
    29701 tcacattcaa tcctaccagc cagtccgctt ttgcccgggc aagagcagat tcatacaatg
    29761 catcatattc tttcgcaggt cttagttgct tgaatccagt tttgattgct ttatctgttt
    29821 gagaagagat ccataaccgc ttcactggtt tattgaaatg cacatactct aagatccagc
    29881 gagcaacaag ttccccttct cttccggcat ctgtcgcaat gactgcttct gagacatctt
    29941 ttcgctgagc cagctgcttg attgctttta attgatgtcc tgttttagga agtggtttga
    30001 ttcctagttt tttagggatc attggcaacg tgtccatttg ccagctctgc cattccttat
    30061 tcagatcttc cggcattttc aatcctaata aatgaccaag ggcccaagta acgatcacct
    30121 tgggtccttc ataataattt ttgtgctttt gattagcacc aagcaccttg cttaggtctt
    30181 tcgcaacgct tggtttttcc gctagaatta gttgtttcat aaaattcctt tctgctttct
    30241 actgtgatgg gctgatgatt tttcaacaac gcaagtccct tgtattgctg taaatcatcg
    30301 tcacactctt cacaccagcg ctcatctccg tcattccaaa aggcgctgag aaagttactt
    30361 tttgcattga tatattcata attttgatgg atttcgttcc attgtttttc gtacgcttgt
    30421 cgtgcttctt cgaaagactc gaatgtttct tcttcttcaa tatcttcttc ccagtcttca
    30481 aaaaaccacc aaggctcatt gtccccgaac atggtaatta cttgatacat actcgtgctc
    30541 cttctttcag cttttctaga ctcatcttag tcacttttca agaaagttgc aagaattttc
    30601 tcttataaaa gggtcattta atgaacatct taaaattgtt acaaatgaat ctgattgttt
    30661 aaggcaaagt agcgataaag ttattgatca tccaaaaata gctgtcttct ggatcgattg
    30721 ttggttcttt tgtaaagtat cctaaaaaac gtaaggaaag ataaccgtgg atcatactac
    30781 gaagttccct gcttcgatga actttttctt ctttcgttaa ttgaaaagcc tcaaatatct
    30841 gcaaaatgat ctgattcgtt tcatggattc catctaataa aacagcttca ttggtatgcg
    30901 gtacactgat cagtaactca tacactgctt gatttgaaaa agcaaaggat cgataagttt
    30961 ctgcatagat ctgcagtgct tcctgaccac tttttcccac aagagcccgt cgcagctggt
    31021 cattaagttc ctgcagaagt ttcgctgtta gcgctgtttt gacatcacga atatttttga
    31081 aatggttata cagcgaggga gccttgatat ccaagcgttt ggctaaatgc tgaaaggtta
    31141 tttgctgaac atccatctct tctgctagtt ctcgaaagca gtcgatgatt ttctcttgag
    31201 acagatttct ttttttcatt tccatgtttc ctctcataaa ataaatcctt cactctttca
    31261 gtttaccacg aaaaagaaag agaaaaaaga gactttcatt gataaactac atattgtagt
    31321 ttataataaa gagctgtaga tgaaaatttt cagaaagaag gagtctaatt ggctgcaaga
    31381 ataaaggaaa aattatttag gaaaagagaa acttgggaac aaaacttgtt tgtcttatgg
    31441 tttggaacat ttatggctgg tatcggattt agtttagtca tgccttttat gtcactttac
    31501 atagatacgt taggtaatta tagtacgtca caattgaact tttggagcgg attgactttt
    31561 tcatctactt tcttggtgac tacgcttatc tctccttggt ggggaaaatt agccgaccaa
    31621 aaaggaagaa aattgatgct gttgcgtgct tcattaggaa tggcaatcgt catttctttg
    31681 atgggttgcg tgactagtgt gtatcaatta gtcgcattgc gtctgcttca agggattttt
    31741 tctggttata tcagtaatgc cactgccctt gtcgcaactg ggacgccaaa agaaaaaagt
    31801 ggtcaagttc ttggaacctt agcaactggt tctgttacag gaacattact tggaccgctt
    31861 cttggcggga tctctgcttc agcgtttggc tatcgcccta cctttttcat tacagggatc
    31921 atcttatttt tcgtcttctt gttgagtctg tttttcgtcc atgaaaaatt tgtcccagtg
    31981 aaaaaagaaa acatggtttc tgccaaacag attttcaaag acttgaaata tccacacgtc
    32041 gtcatcggta tgttcgtcac aacgatgatc atccaagcat ccaacaattc cattagtcca
    32101 atcatcagtc tgtacattcg ccaattgctg catggacatg ggaatgttac gttgatcagc
    32161 ggaatcattg cttcgatacc tgggattgcc actttgatag ccgctccaag atttggtcgc
    32221 ttaggtgaca agattgggag tgaaagaata cttgcgattg gtttgggttt tgcgattctg
    32281 gtttatatcc caatggcatt cgtcacgaat gtttggcaat tagctgcctt acgttttttg
    32341 attggtatct ctgatgcttg tctgctccct gctgtccaag cattgatcac aaaatattcg
    32401 cctcatcaag ctgctggacg gatcttcagt tataaccaat ctttccaagc aactggtaac
    32461 gtagtagggc ctatgatcgg ctccagtgtt tccagtgcat ttggttatcg tggcgtcttt
    32521 ctttcgactt ctcttcttgt tttggtcaac ttcttgcttg tacgacacaa tacacaagag
    32581 atccatcagc aagaaactgc agaagaaaaa gcttcaggag tctccatagc ttcagggcac
    32641 tctccttctc atagacaata agcgtaaatg aaaaacactg cttgaacagt tgtcacagac
    32701 tgtccttgca gtgtttttgt ttaggcctgt tttttcaaga actcagataa gaggaataat
    32761 cccttttcca aggtttcttt tttacaacag taacctagtc gggcatgctt cggtaaatca
    32821 aaggcacttc ctggtactaa tagtactcct gtttcttcta ataaattgat acaaaatgtc
    32881 tggtcatctt cttcgatggc taatttaatg aaagaagtag agacgtaatt gggagcgaca
    32941 agttcaactt tcggttcatt ggctacccat tgttttaaaa tcacaaggtt ctctgtaatg
    33001 atctttttgt ttcgcgccaa tattttttct ttattcttca atgcatgtac agcaagatca
    33061 tctgacagga ctccgccgca aatcatcgtg tagtctcgat actttcgaaa aacttctgcc
    33121 aactcaggtc cagtagctgt ccagccaata cgaatacctg gaatggagta ggttttggat
    33181 agggaattgg ttgctatacc tttttcatag acatctacga tcgacaagaa ttcaccttta
    33241 tccgttaaag gcgcatatac ttcatctatc aatacatatg catctactgt acgagcaatt
    33301 tctgcgattt tttctaaagt tttccgatct agcaatgtgc ctgtaggatt gtttgcacta
    33361 ttcaaacaga tgatttttgt ttcaggtttg actaaggctt ctagctgttc taaatcaaac
    33421 tgccaatctt cttcttcctt caaatgaaca aagtcaacag cggcacctaa agatttgggg
    33481 atgtcatata attgctgata agtaggaagc atcgaaacaa catggtctcc tgcttccact
    33541 aacgcgtata aagctaaaag attagcacca gtcgctccat tcgtttgtaa gatgttttcc
    33601 ggatcgacgg tttgataaag agaagcgact tcttgtttga aaacatctga tccttcgatc
    33661 cagccatagt ccaatggttc gtttttgcgt ttttcgaaaa atttacttac gctagtccca
    33721 tctaagccaa cgagttcgtc taatgtcaat gcttcgatcg agctttgtgc aatatcatat
    33781 accgctttct tttcccattt atttagccat tcttctacac cgaaatttgc gattttcatg
    33841 tttctttccc ccttatatct tgatccagcc tacagcaagc aacccgattg aaagcagtgc
    33901 ggccacacaa atcagcccaa tcattacttt ttcttttccc gtaatttgat gtttggtttc
    33961 acgacaagcg atccagtaga agcaaaaacc aggaagatag gcaatactac ataaaaggac
    34021 ataagaaaat cctgctaaaa tgatgcccat caattcaaaa actacagcaa tcccaccgat
    34081 cactacttgt tttttgattc cttcttttaa agaaaactgg acttgataga tcccgacaag
    34141 tagataacaa atcaaaattg ccgctgtaca taaagaatag gcaaattgat aggcataatc
    34201 agtaactaag aaagtcaata agaatagatt agtcaagatt gcagtaacaa ctaacgaata
    34261 agttggggca ttttttttat tcgtttttcc ccaacctttc ggcaacagac cgtctcgagc
    34321 catcaacatc gttgtttctg ctgggagcat cgtccaagac aaccaagaac caatgatcga
    34381 aataatcaag ccgatattga tcagcgctgc tccccaattt cccacgattc cttttaaaat
    34441 atttgccata gcaggttgtg aaatagttgc taactcttct tgagataaga cgccgtatgg
    34501 caagatcgaa gcaaggatat agataaacaa taaacaaagc agaccaatga tcgatgcttg
    34561 ctgggcttct gaacgtttct tcgcgcggtt ggctaaaaca cttgcgcctt cgatccctac
    34621 aaagacccac atcataacca tcaaacaacc tttaatctgc aaccagatcg ttccttgctg
    34681 ccctgttcca ttcactagat tatcagatac gttaccccaa aaatcagcag tgaatatatt
    34741 cactttaaat gcgataaata caaatattaa aaataggaat aatggcacta atttacagat
    34801 cgtcacgatt gtattgatga cgctcgctcc ttcaactcca ttgtttacta ataaagtcag
    34861 accccaaata aaaatacttg caaataaaat agaaggaaca ttttgtccac ctttgaatat
    34921 cgggaaaaat tctcccatgg cgctcatcag catcgttgca aaagcaacat ttcccagcca
    34981 cgcagacaac cagtaacccc aaccactgat gaagccacct aatttaccaa atgaagcttc
    35041 cgcgtaaccg aaaataccac catctagatc tggacgcttt tctcctaaat gattcagcga
    35101 taataccaaa gctaaaatac caatcccaac gattatccat gaaaggatcg ctggccctgg
    35161 agctgcagaa ctagccagat cacttgtgat cccaaacaca ccgcttccga ttgacgaact
    35221 gacaaccaga gcaaccagtc cccatttgct aatacccgtc gatttctttt ccatttccat
    35281 caccctcact tataaaattg aacaaatcac ttgcaaaaga ttcccttcta ttttgcaact
    35341 gctcttgctt aacaaaaagc aactttattc atattattgg atattatgaa ctttttacac
    35401 aagtatttca agtataaaat gaataaaaat taattttatt gcaaaaaaac agctatgcta
    35461 tccaaaagaa aaggtgtttc ttttgttagc catagctgtt ttgattcatt taatatcgct
    35521 taaacgtatt gtaaaacttt tgtttcttga tatgcgtggt cgatcaaacc accacccaga
    35581 cattccattc catcataaaa tacaactgct tgtccaggag tgattgcacg tacaggttcg
    35641 tcgaatacga cttctgcacg attatcatcc aataagcgaa ctgtcacagg aacgtcttgc
    35701 tgacgataac ggaatttcgc tgtacacttg aattcttttg gcattggttc attcgtcgta
    35761 aagtggattt cacttgcatc caagcttgtg gcatacaatg caggatgatg gaacccttgc
    35821 cccacatata atgtgtttgt cgctagatct tttcctacaa caaaccaggg ttcttgcgaa
    35881 tctcctccgc caccgatccc tagtccttga cgttgaccaa tcgtataata tatcaagcca
    35941 gcatgttgtc ctttgacttc accatcaaga gtgaccatat ttcctttttt tgctggcaga
    36001 tagttgctca aaaattgttt gaagttcttt tcaccaataa agcagattcc cgttgaatct
    36061 tttttcttag cagtcgcaag gcctgcgcgt tcagcaattg cccgtacttc tgatttttcc
    36121 atgccgccta gagggaacat cgtttttgaa agctgttctt gtgataattg gctaaggaag
    36181 taagtttgat ctttattatt gtctactcca cgaagcatgt ggaccacgcc gttttcatcg
    36241 cgagtgactt gcgcataatg tccagtagct acataatcgg cgccaagatc cattgcataa
    36301 tccaagaatg ctttgaattt gatttctttg ttgcacatca cgtctgggtt aggtgtacgt
    36361 cctgcgcggt attcggctag gaaatattca aatacacgat cccaatattc tttttcaaaa
    36421 ttcacagagt aataaggaat cccgatttga gcagctactt tcgctacatc cttataatct
    36481 tccgtggccg tgcatacacc gttttcgtcc gtgtcatccc aatttttcat gaatattccg
    36541 atcacgtcgt atccttgttc ctttaacaaa agtgctgtta cagatgagtc gacaccgccg
    36601 ctcatcccta cgacaacgcg tgttttgctg ttgtctgtca tgatatcacc atcatttcaa
    36661 ttagattctg aaacatctac gagttgaagg tcgcaacttt tcagtatttt acattatacg
    36721 tcatatttct gaaaatttca tcccttaatt atcaagtcgt aaaaatactg ttctactgga
    36781 cctattcagt acacaaacat gatagtcaac caaaaaatca ctcatctttt gagcattcga
    36841 tataaaaaat tggcatactc ataaaaaggg ccagcctttc ggcaataagg aataatttta
    36901 taaattgtga aaaactattt ttcaaaattg cttcttatcg cttaaagctg cacgtctcat
    36961 ttacaacctt ttctttttag atgttatgag ttgctcagat aagtgatttt gtttactcta
    37021 agaaaaaagg gaggcaaaag atacatgaat tactttatca gtgatatgca ctttttccat
    37081 gaacgcttat taggaaaaaa cgactttgct ccgcggctct ttgcaaatgt ggatgaaatg
    37141 aaccaacagt tgatcactag ctggaatgca gtaataaaag aaaccgacca tgtttatcat
    37201 ctaggcgacc ttgctatgca tccgcaatat gaaaaagcaa atgcagaaat tttggaactg
    37261 gtcactcaac tgcaaggaac aatccacttg atcaaaggga accatgacag tcgcgctttt
    37321 ttccgttatt tagaaaaaaa tgatcctagg cttactgacg gaagtcccaa attttatttt
    37381 tatgatgtag gtgccatcgt taaattcgac catcaacaat attacttgac tcattatccg
    37441 atgttgctcg ggcctaacga cactatccgc aatcttcatg gacatatcca tcattacagc
    37501 gtacctatcg agaacgatgt caatgtaggc gtagacgcac cggaaagaga actcttaaag
    37561 gaaaaactac catttggcac tccgttatct gagcaacaaa tcaaagaaat ctatgaagca
    37621 aagaggaagt acgataggtc gtgaaaaaac tgccctatat caagtattaa acaaataaag
    37681 ataattactc atatttttca tttgagtagg cttattgcca caggaacatt aggatcagaa
    37741 atggagttgt aacaagactt ctgtcacaac tccattccat actattcgtc tttttgctca
    37801 tggtgttctt ttaccagttt tttctgtaat tctttatcat aatttcgggc agctgacgtt
    37861 aaaaataaaa cgattccgca taataaaatc ccgattccgg aaaacaagaa cattttttct
    37921 acccctaaac gatcagctaa tggtcctgca aataataatc caattggacc tgccaggttc
    37981 atcaaagagt tgaagttccc taagacacga cctaaaacat tcgattcata actctgctgg
    38041 atcattgcca tcagcagtgt attgaaatat ggcgttgcac aacctgctcc tgcattcaag
    38101 ataagaaagt acaagaaccc ttgactatcg tttggaacca atccgcttgc accgatcgtc
    38161 aggcccataa tgatgtaggc tataataata ggtttcattc ggtctttcca tgttcctata
    38221 aaactgatca gtgcaccgcc taagagcatt ccgactgcgt agactacttc cactgctccc
    38281 gcctgtccaa cggaaccacc aaagtaatct agcgtcatca atggatacag actgattgct
    38341 ggcatgaaca acagcatcac aaatgcacca ttgatcgtta tgtaccataa ccctttatta
    38401 tccatcaatt gctgcagccc aaattttgca tctttaaaaa tagtaagcgg aacttccaat
    38461 atttcaggag aaacttttgg gatcttcaca aaaatcaata agccgacacc aaaaactgcc
    38521 cccaatacat ctaataaaat aaggtaattg acaggaacga ctgaaaacaa agccgcacct
    38581 agagctggcg caatgatgaa attcgctgat tggaccattc ctaactgtcc attcgtcctt
    38641 gttatgtgag aagatggaac gattgtcggc ataatcgact ggatcgttgg catttggaat
    38701 gtttgtgcga ccgaacgcat aaacaaagac acaaaaacta gccaaatcgg gaaggattct
    38761 gaaatcgttc cgactactgc taaaataagc gcgaaaatcg ctacaataat atccgtgaca
    38821 atcaaaagag cttttttatc ccagcgatcc actagcggtc ctacaaatgg gcttagtaaa
    38881 accatgggaa tcattcctag taaagtagca aaactcaaaa ttgtcgccga accggtttct
    38941 cttgtcaaat accagatgat cgcatattgg acgaccatac tagtgattcc tgacaaaaac
    39001 tgtcccgata aaaagaacaa ataatttctc ttccaattcg ttaaaacgac cgactgttca
    39061 ttttccataa acatccctct tttctttttc attttgtata gcgtttgcac aacaatcatt
    39121 atacgggatt cttttttcaa aaaatacctt tttccctaaa taacaatcgt ttcaatttaa
    39181 atttctttac ttagaaacaa taaatcagca aaatattaaa aataacaaca tgtgttaaat
    39241 ttaaaaataa aaaagacaac taatttgcgt cgtccttaca aagttagtca tctaacaata
    39301 ggccgtttca aaactagttg tctttttata tccacagatc attcgacttt ttcgaataac
    39361 ccacggctaa tcatcccatc catcagaaaa taaaatttat cttggctcat tttctgtttt
    39421 ggatctttga aaatattgac tgctttcaat ccatttgctg tagtgatcga cattttcaat
    39481 gtttgaatat cttttgatac agtgattttt aatttgaatc cttctttgct ttgcggcgtg
    39541 gcatctaatg gtcggatcaa ttgataattg cctccgtttg tttctgtaaa tccattatcg
    39601 cgcaatgtat atctttttgc atcaggatgc aaacgatagc taattggtgc gcccagtacc
    39661 gttgctgttg tttcaaatgc catgattttc acctcgataa aattagtttc tctttattat
    39721 aaaagtttat cctgattttt actaggtttt tcagttaagt tccctaaaaa atgaaggcgt
    39781 tctctcagac atctgttcat ataagaatga cttcactagt tattttttga cagcacgaac
    39841 taattgatca aggaaatact ggatgtcgtc ttcggtattt cccaaaccaa agctgatacg
    39901 gatggattct tttagtatcg gtgtatcttc tccatacatt gcttctaata cgtgtgaagg
    39961 ctcgatattt cctgctgtac aagctgaacc agtagaaata gcgaccccat taagatcaag
    40021 cttcatcagt agcaaatcat ttgcttcgcc aattaagcga agattcaaca catgagggag
    40081 tttattcgtt aaatcaccat ttacttgata agggatttcg tttgcttcta atgtatcaag
    40141 aatcaattgc tggaatcctt tatatttgtt gttacgtgct tcacgttctt caggtgacag
    40201 cagttcaact gcttttgcca taccccagat ccctgctaaa ttttctgtac ctgcacgccg
    40261 cttttcttcc tgctccccgc ctaaaagcaa tgcagggata tttactccgt ctcgtttata
    40321 taagaaaccg acgccttttg gcccattgat tttatgagca gagatactta ataaatcgat
    40381 tcctaattct tctggatgga tcgtttggtt tccgtacgct tgtaccgcat ctgtatggaa
    40441 ataagctgga tgatccgcta aaagttcacc gatttctttg attggcatca aatttccgat
    40501 ttcattgttc ccgtacatca cagagaccaa gatcgtatct tctcgtaacg cttttttgac
    40561 ctcttcgatc gacagattcc ctttttcatc tacaggcaga tatgtaacgt caaacccctt
    40621 tgtctctaaa tatttcatgg tattaagaac agcaggatgt tcgatagctg tagtgattag
    40681 atgtctgcct tctttttgac gcgagaacgc tgtttctaag atagctgtat tatccccttc
    40741 tgttccccca ctgttaaaga tgatctcgtg aggttttgct tgcagactgt ctgcaatgat
    40801 ttgtcttgct gtttccaatt tttcatgcgc gtggcgacca aatccatgta tgcttgaagg
    40861 atttccaaac gtgtcttgca tgattgccgt catcgcttca attacttgtg gatgcatggg
    40921 ggtagttgcc gcatgatcca gataaatagt tttcacttca ttcatccctt ccaaattatt
    40981 gctaggcaca ttctagcata aaatagcgaa caaaccaaag aagagctcat ttttgtctag
    41041 aggacgtttc atgggcatag aaaaaaggag ctgtgacaaa agtcttgtcg cagctccttg
    41101 aagctgctca cttctgtcac aacttctttc cctctttttt ttgtttattc gaattctttt
    41161 tcttccagac ggaataacgg actcatcggt gtattttcat ggatgcgttt gaccgcttca
    41221 cccattaatt ctgaacatgt aacgattgtt aagttttctg gatgacgatt ttcttctgtg
    41281 aaaacagaat cagtgataca aatatctttg attggtgcat tgtctaaaat acttttcgca
    41341 tgatcggaca ataatccatg tgacgcacag gcatatactt cttttgcgcc gttttctttt
    41401 aatacatttg ctgctcgtgc aagcgttgct cctgtgttca agatatcatc taccatgata
    41461 catgtctttc cttgaacatc accgatcaca taaccaccct cagttgaagc atcatctgca
    41521 tgatctacga tggctaatgt gctgttcaaa tattctgaaa gactacgtgc acgctgaaca
    41581 ccgctgtttt ttggcgaaac aactacgatg tcatctcccg tcagacctaa ttgacggtaa
    41641 taatgagcaa acaacggcat cgtaaataaa ttatctacag gaatatcaaa gaatccttgt
    41701 acttgaaccg tgtgcagatc taaagtcagt actcgagttg cccctgcttc ttcgatcaag
    41761 ttagcgatca atttcgcagt gatcggttca tgaggtttcg ccgtgcggtc ttgcctagca
    41821 tacccatagt aaggcaatac gacattgatc gttttcgcac ttgctcgctt cattgcatcg
    41881 atcatgatca gtagttccat atagtaatca ttgacaggtg cattcgttga ttgaacgata
    41941 tacacatgat cccctcgaat actttcttcg atattgatgg aaatctcgcc atcactaaat
    42001 tgcttcacga cgcttttacc taattctgtt ccgaacactt tcgcgatttt ttcagctaat
    42061 ggacggttgc cattcaaact aaaaatcttc aacgtgtcat cctgatactt attactcatg
    42121 tagtcctccc caaaacttct ctattcttcg atagtttaac atatttttcc aagattttct
    42181 gttaatttca gcaaattctt aaggaagtga aactgttccg ttactccaac tggcaggaat
    42241 gactttatga taatcgcccc agttgtcgtg tactttgtaa taacgttctc ctgtttcagt
    42301 ttcaaaataa gcgtacgctg tcacccaatg attgccatat gtactgccaa gtactttcaa
    42361 aataccaatc atcacaggtt tgccttcacg tatacgtttt gtagctcgct gccatgaacc
    42421 tagaagagtt gctcgtgcac gctcatgatt gccattcttc tttaaaaaac tagaaatccc
    42481 tgtactaacc tgaaaaggaa tcgtaggaag tcctagtggt tggatctggc tacgcaatgc
    42541 cttcgtcaaa accaacgaat cagccgtatt tttcttgcga atctcatttg gaagcatcca
    42601 ccccttacga tagtcttgat aataggcaag gagaactgct gccgcgtaag taccacaaag
    42661 atagcctgag ggggtagccc atttcttata cctcaaaaaa cgttcttctg gcaatcccac
    42721 ccagcaattt gcgagttcct ttcctgacct tttttcttcg atcaaaacgg gaatatcttc
    42781 ttttctgttg atccatctag ataactcgat tggctcttca ttgatcagct tccatttttt
    42841 acaagaaaga ccctcttgat aagtatattt tgctggagaa tattctaccg gatgtcctga
    42901 ttgatcgata atcacataac tttgatcttc aaaaaccaga gctaaaaaaa gcaatttact
    42961 ttttcttgac tgcagctgct gagtgctggc taatttttta atttttttat ctactttgaa
    43021 atataaaggt ttatccacta cttgattcag ttgggatcgc ctcctttttt tcattataag
    43081 tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt ttatgttcta
    43141 tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag gagcgatcga
    43201 tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga agaatggcaa
    43261 aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga tttagttgac
    43321 gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc agaaaaagtg
    43381 tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga ttatgaccga
    43441 agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga atcattgcag
    43501 tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt accattgcca
    43561 tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt ctttgcttat
    43621 ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc caacggtgca
    43681 caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc cggcaaagaa
    43741 aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt gattgctcct
    43801 catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc gcaatctttt
    43861 gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac gggtttgatt
    43921 gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca tttgaatgac
    43981 atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa aacagtacgt
    44041 tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta ttctcgcgta
    44101 ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct tgcctctgga
    44161 caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga agctgctaaa
    44221 aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg tctagaagaa
    44281 aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa taacttaggt
    44341 atccaagtag gctaccccga taagatccca gcattattcg accagttcaa aacagtacct
    44401 gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact cacattaaaa
    44461 gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat gagtgctgac
    44521 acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc agcgatcctt
    44581 caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg aatcggagct
    44641 gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt tgacgaatat
    44701 ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa caaagcacaa
    44761 gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa cggaaaatta
    44821 accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga agcagcaaaa
    44881 acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat ttggcgtaca
    44941 aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc ccctgctaaa
    45001 ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt tggtatcacg
    45061 gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta agaggttgtg
    45121 ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt ctcaaatttt
    45181 tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt attagaggtt
    45241 gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg ctttccacat
    45301 ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt tatcgagagg
    45361 ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat agttcctcat
    45421 attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga
    45481 gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa gcagcaagga
    45541 gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca tttccttcta
    45601 tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca cttatttttt
    45661 tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag ctaatgctag
    45721 caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt tttcactttc
    45781 ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg gtgattcatt
    45841 tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag tttctcgcac
    45901 agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt ccccatcaaa
    45961 tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg ctgaaaaaat
    46021 agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga tcttttctgg
    46081 gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag ttgacacaaa
    46141 aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca caccatttgc
    46201 ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg gttcttgtac
    46261 gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa tagcacctac
    46321 agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg gtttaatctg
    46381 catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct gctgaccttc
    46441 tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc caagagtatt
    46501 ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga tttgatcttc
    46561 gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc ggaaagataa
    46621 gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag ccacattata
    46681 tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc tattggaatc
    46741 gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag ggcccgttaa
    46801 agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc ctggtaccca
    46861 gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac ctgtttcatt
    46921 tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac cgtcaaatac
    46981 atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa atcctggatc
    47041 ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg cttcgaaagg
    47101 atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag catgaatctc
    47161 ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg cactatttgt
    47221 aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat ttccagttcc
    47281 tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca actgaatcgg
    47341 gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga aggaataagt
    47401 tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt gtgtcagaga
    47461 taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta cataaactgt
    47521 cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt cataactagt
    47581 catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga aagtaaattc
    47641 gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag tgatagccaa
    47701 gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca ataatccgtc
    47761 agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac tagaggtcac
    47821 agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat ctgtcgcagg
    47881 atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg ctgcagcaaa
    47941 tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat acatgatgtc
    48001 agaagccatt tcttctcctg tttcaggatc gtaataatat gcctctaaac cagtattcgt
    48061 ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca gattcccatg
    48121 accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg ctgaagtctc
    48181 taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg gatcttcaga
    48241 tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt tgctgccgct
    48301 cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac tgcctagtat
    48361 ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta ctgcgtctct
    48421 taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta tttcgatcgt
    48481 cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg tcattccatc
    48541 tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca cgccgtcttc
    48601 taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa cccctttcca
    48661 ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt ctttcatcat
    48721 aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtattt cctccattgt
    48781 tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt gttcgttagc
    48841 agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat tcatcattag
    48901 cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa attagaaagc
    48961 gtttacttaa agtattacct aatttatttt tactgtcaat aaaaaacaaa attataataa
    49021 gtgtatttta taaaaagaaa aaatataact taagttgttg ataataaaga gatttattaa
    49081 cgtaaaaaga ataagttttt ttaaaataac gtataaaaaa gagcataaaa caaagtcgtt
    49141 tgttttatgc tcttctttta ttattttgtg ctttttattt tatcaaatca ctttgttttt
    49201 ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt catgtccaac
    49261 gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta gattatacgc
    49321 atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat aaatatctgt
    49381 gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg aataatgccg
    49441 tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac tttttaaaga
    49501 gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata agcggttctc
    49561 cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa aatccttttg
    49621 atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc ctgctgtccc
    49681 agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg agatcaatgc
    49741 gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat ttctgctgat
    49801 cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg atctgctgtg
    49861 tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt ttgtccaaca
    49921 gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat ttctttgatt
    49981 ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt tgcgttcaaa
    50041 ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc ttcataatat
    50101 agaggcattc ctggtaattg tcctgggttg tagccaaatc ctgttgcatc tgcatcaaaa
    50161 taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc gattccgcca
    50221 gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag acttgtggta
    50281 atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg gttacctaat
    50341 gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct taggttctct
    50401 ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat ctttttaatt
    50461 ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag gataacaata
    50521 ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc gctgatcctt
    50581 gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc aatctttttt
    50641 tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc gcttgcttgt
    50701 gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc cataagccga
    50761 tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat cgtctttgca
    50821 tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag atgatacaac
    50881 tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac cccttgtgca
    50941 tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga agtaagatgg
    51001 ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc tgtcatttcg
    51061 ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat cactttttct
    51121 aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa agcaatgatc
    51181 ccgacaccgc caggatgaaa taccagttct ctcttcgaag ttcctccatc tggcaaacga
    51241 acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg actgattgtt
    51301 ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt aatcataact
    51361 gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc atagataaac
    51421 cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt atagtagtcc
    51481 gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca acgataaatg
    51541 acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat tggtcttgct
    51601 atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat tgataggtgt
    51661 agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac aagaagaaat
    51721 tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga cagcgaatga
    51781 aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc aattgcaaat
    51841 gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc cagtcaaagc
    51901 agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg aagcaagtca
    51961 gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata atgagatcaa
    52021 tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg cgcagatcat
    52081 cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg aggatctaga
    52141 tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata ataaatatga
    52201 accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca ttcagatgat
    52261 gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa tcgagaacag
    52321 attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag atgttacacc
    52381 agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa cagatacgct
    52441 gaccacttca caacaagcgg agattgatca gctccaagag caacagacag ctgctcgttt
    52501 aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag caaaaatcga
    52561 tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa aattaagtga
    52621 attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg taggagattc
    52681 cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc gtgccggaga
    52741 agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg aagtgacagc
    52801 aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag ataaagaaaa
    52861 agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa acaaagacta
    52921 ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag aacttcaaac
    52981 aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga tggatgttca
    53041 gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc atgatcttcg
    53101 cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc aaaacacaaa
    53161 tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc tgtggaaaaa
    53221 ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg ctcaacgcca
    53281 agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga aaatctcagc
    53341 catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc ttcaaaccac
    53401 tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag aaggaaaaga
    53461 aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga aacaaaaact
    53521 gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc ccaacaacag
    53581 tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc cattggagtc
    53641 tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat cagtccaata
    53701 accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat caatacaata
    53761 caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa ttctccagct
    53821 tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag catcaaatag
    53881 gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa tccggcaaag
    53941 aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg cttttgatcg
    54001 atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag taaacgagca
    54061 agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc tacagcgatc
    54121 accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg gtcattgact
    54181 cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat gtaaaggaca
    54241 gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg gtttttgact
    54301 tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac aagagctgca
    54361 ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc aggtttaacg
    54421 aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata taccatggca
    54481 ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac gaatatcact
    54541 acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc gccagttaat
    54601 gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc agaccatccg
    54661 gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc tgtcagccag
    54721 gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat cactggcact
    54781 cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc agctaagaca
    54841 aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt caccacaaat
    54901 attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt ttttagttct
    54961 tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa atccattgaa
    55021 gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata ccctaaaagg
    55081 ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc ttttttcgtt
    55141 tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc catttgtgaa
    55201 agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca gatgtttcct
    55261 aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac tttaacttta
    55321 cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa gataattgac
    55381 atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc aatcgtagag
    55441 aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt atctattttt
    55501 ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc taaagcaata
    55561 ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc
    55621 gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac acccatgtac
    55681 ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga taaaaatccg
    55741 atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac taaagaaaac
    55801 agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc aattttacca
    55861 cttactaaac caatactacc ccatgccaac atagggacta atgctattaa aatttccata
    55921 accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt aacaacacaa
    55981 gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat ttaagattgg
    56041 gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca aatggatacc
    56101 taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat gtgttttgct
    56161 aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt cttccgctgg
    56221 gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct tttctatgct
    56281 cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca attggttgaa
    56341 gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa cgatctcgta
    56401 gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct gaatgattcc
    56461 ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata
    56521 caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta atgtatactt
    56581 ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg aaagtggcac
    56641 tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat tactgattgc
    56701 tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt ctaccagttt
    56761 ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt tttgtatgtc
    56821 ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat aaaaaatcag
    56881 aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt tgatgtttgg
    56941 cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca agatcaatgt
    57001 ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt tcagggaacg
    57061 atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg ttcgattttt
    57121 cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc
    57181 cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt catcaaaata
    57241 aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat ttccatactt
    57301 ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga taattctgtg
    57361 tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc gatttggaaa
    57421 tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc catattcgta
    57481 ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt ttgacaccaa
    57541 taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg ttcttctctt
    57601 tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc
    57661 tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac catgattttt
    57721 gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa gcccttgtga
    57781 aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat
    57841 gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta ttcttaataa
    57901 ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt tcgataacac
    57961 caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag taatcgataa
    58021 agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa gggaactcgt
    58081 cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg
    58141 actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag gacaacaatt
    58201 ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa tcggtcatct
    58261 tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct atgggcggta
    58321 atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag gactgtcgct
    58381 cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac attgattttc
    58441 ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg attttggaca
    58501 tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat tactgatgta
    58561 tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg tttttcttct
    58621 ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa ataattgctg
    58681 agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa catcactgcc
    58741 ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat tttctttttc
    58801 attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc gattgctgca
    58861 gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc agctgtacgc
    58921 tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc caccagtagc
    58981 aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt ctactcctac
    59041 gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa aggctgcctc
    59101 acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat aggttccata
    59161 atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg ttgcttgtcc
    59221 gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag gcgactcccc
    59281 tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc ttgcatcttt
    59341 aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg ttgtgacttt
    59401 ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga tttcaataac
    59461 cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca aattatacgt
    59521 gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta ctttggctac
    59581 tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt ctactatata
    59641 atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct gatcataata
    59701 agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag cgcttccgtt
    59761 ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag aagccgtttt
    59821 tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat taataatcgt
    59881 tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc tgatttcgtt
    59941 tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac catcccacac
    60001 gccatccacc atatcaggca tcatcactgg catcataaca accattggag acgcgattct
    60061 ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc tagaagaagg
    60121 tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat tcgttgtttg
    60181 tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc gtttttgaat
    60241 ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta aaggattatc
    60301 tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat
    60361 attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt ccggaacaga
    60421 tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg cgacttcttc
    60481 ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga gtaacagtac
    60541 actcattccc gtgacaatca tttttttatt catttttttc tccccgttct ttttttatta
    60601 ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat
    60661 tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga ccactcggct
    60721 ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt gttgcggatt
    60781 cataggcaga gactggaact ttcccggatt ctaattcagc catctcctgt ccgttaacta
    60841 ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg acctcgattc
    60901 tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca aaataatagg
    60961 tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt
    61021 tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat tgatttttca
    61081 agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc tgatatagag
    61141 caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga cgtccatact
    61201 tgaagaagta tggatctctt agataaccga catttttagg atagatatga atctcttgca
    61261 gttcctcggt tgattctgtc gggtgataaa ttggcaaaat agcagcaatt gggatagctg
    61321 tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg aaaatcaaat
    61381 aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc ccgtctccta
    61441 atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca agcctttctt
    61501 ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg acgaattctt
    61561 taggagtcat gttttcagca gctttttttt catgataata atcggtcatg tcgtaaatgg
    61621 caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt
    61681 cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta taatcgatat
    61741 ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat tcttctgccg
    61801 acatcgcatg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat tgaagcatca
    61861 aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga caaacaaacc
    61921 ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga ataatcgtcc
    61981 ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa gctgtaatct
    62041 gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag gcgtctctgc
    62101 ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt ttcctggaac
    62161 tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa taaagtaacc
    62221 tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga ccccttccaa
    62281 caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc ctccctcgat
    62341 gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc cttcaagacg
    62401 tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta gttcatcttc
    62461 taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg gagcagtttc
    62521 gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg caaactggaa
    62581 cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct catattcacc
    62641 aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta ctcctacggc
    62701 acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt ctaacggatt
    62761 accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga tatcgaaacc
    62821 tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct cccgataatc
    62881 aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg ttgtatacgt
    62941 gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta tagcattatc
    63001 gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt ctgggttctg
    63061 gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct tataaacaaa
    63121 tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta catatttccc
    63181 gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt cgttcacacc
    63241 agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg tttcataaag
    63301 ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat tgatattcat
    63361 gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat catactctgt
    63421 gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt cataatagga
    63481 attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt catctacaag
    63541 ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt ggaattgata
    63601 cgtctcatct actaactgat aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc
    63661 gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc
    63721 gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg gattttggtc
    63781 atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct tgctgacttg
    63841 gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca tccgtccagt
    63901 agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt tcactgtgca
    63961 aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc tgaccatttc
    64021 cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc cgacatacgt
    64081 tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat cccatatttc
    64141 tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact gaatatctgc
    64201 cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg
    64261 ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa tgtcattctt
    64321 gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag caatattttt
    64381 caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc catcgattcc
    64441 taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca gccaatgatg
    64501 ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc cattcgtatt
    64561 gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt cttttacctc
    64621 tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag atgcataggt
    64681 aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa aatttctgaa
    64741 caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc tccaagctcc
    64801 attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc
    64861 tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt ctttatacaa
    64921 tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg gtgtacgatt
    64981 taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc
    65041 cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat tcaccatacc
    65101 gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt ttactgcctc
    65161 aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac tcatactcgc
    65221 tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct cgcccccgat
    65281 gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat cagatgctgt
    65341 gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat aatccaagta
    65401 actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg gaacgtttct
    65461 atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc gccagttcgc
    65521 tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg gcgagccttt
    65581 gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg tatagtcatt
    65641 cgttgtaaac ttcatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt
    65701 tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat
    65761 aaacttttcc gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat
    65821 cttcatcata ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg
    65881 ctgccgcatc gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag
    65941 ccagatgcca ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt
    66001 cctcatctaa gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg
    66061 tcgtcatatg atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact
    66121 gcaaaagctt caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt
    66181 ctttcacaac aatcaacaaa acaatgccca tctaattata atttaattta attttaattt
    66241 taaatcaaat gatatccttt ttagttaaaa acaccaatta tacaacattt attattaaaa
    66301 ttaaattata aaattttata acgttctcat ttaacaaaac ttaagttatt aagacaaatg
    66361 aataaacaac tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac
    66421 tcatgcttta ttcgtacaat taattcagat aacggcagcg cataggatgc caataacact
    66481 tcctaaaata gctcctccta aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga
    66541 ataagcgatc aaacaagcaa ataaaaaggc taatattcct gcaactggac ttaattcgaa
    66601 caacgcagct actgataaaa aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt
    66661 cggtttactt gtgtaaagga caagccgacg atttttttca taaggtcttt tccgtcttgt
    66721 cgtttttttg ataaaaagca aaccaacaaa aaaatagatc aaactgccga taagaatggc
    66781 tagaccatat tctctagtat caggtatcaa gactaaaaca gcagcaagga gcaaccaaat
    66841 aaaaccacca ttagagatat tactgataaa tttcaaaaaa ttgatttttc gactatccgt
    66901 tttccgttgc ttggcgaact tgttgataat actatcatcg attttagcaa tcaaacgcaa
    66961 catgttattc ctccttttta agaacctttt cttgcgtacg atggataaaa agcaggactt
    67021 gaaaataaaa caagaaactg aaacctaata aagcaaaata gattggtcct gcaaaccaga
    67081 atgcgacaag aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa
    67141 aaaacaagaa aaatgcagta atccaagaag cttctttttg ttccagttga agcaagattt
    67201 tgtaactaaa gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat
    67261 agccaattcc cccaccccaa tagcggaaaa tctgataatc gatcaataga agaagaaaat
    67321 aaacacttgc taaactatct tgctttccat aaagagagaa gttctcacga aactttttcc
    67381 agaatagtga accgagcttt ttctcttcat tttttttgta atgataaatc atatacagaa
    67441 ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac catagtagct
    67501 gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca ttggcttttt
    67561 taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc cttcaatgaa
    67621 ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc
    67681 catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa
    67741 cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat tccaagctgc
    67801 gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg
    67861 actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca attcattatt
    67921 gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga agaacgatgg
    67981 aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata catattgagg
    68041 gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa acatcagctt
    68101 tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg aaaggacatt
    68161 accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa
    68221 ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa aggtcggagg
    68281 ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa gcaatggaat
    68341 gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt ttgtttgccg
    68401 attcacactg gtctttttga aataatccat catttgccgt cacctccaga ataatagacc
    68461 cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag aaacaggatc
    68521 attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt ccacatatgc
    68581 agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt catgacataa
    68641 gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta aaaaataata
    68701 ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc tccatcgatt
    68761 tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta aagtagcatt
    68821 gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca
    68881 aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat caatcctgca
    68941 gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa gactgccggt
    69001 aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag tactgtggct
    69061 aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata aacggtgttg
    69121 tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt tgctagacca
    69181 atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac tgaaaaaagc
    69241 ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa taaataggcc
    69301 catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg cctcctctgt
    69361 acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta ctgctttttg
    69421 tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt caatctgttc
    69481 attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca ccgtattttt
    69541 aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt tgtcattatt
    69601 cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt cgccgaattt
    69661 ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct caggatcttt
    69721 tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc ctgaaccttc
    69781 ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat tttccatcaa
    69841 actagagtaa taattgatat tttgggcacg catggctact aatccggaaa taaatggatc
    69901 agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc cataataatc
    69961 ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa tcttgacttt
    70021 ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata catcagcccc
    70081 taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt ttttatctag
    70141 tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt cactgattcc
    70201 agcttcttta aataactttt tattataaaa aatgacttgt gtatctgtat tgaatgggat
    70261 cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa aatccttaga
    70321 agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg ctctttgggc
    70381 aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta ttgcagataa
    70441 agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga tctcgttttg
    70501 tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac cggatcccca
    70561 aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag aatttgctgc
    70621 acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa acaatgctga
    70681 acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa ttttaatgta
    70741 accgatgccg gttttaatat aacgatatca ttttgttctg aaaataacaa tttataaggt
    70801 atgattctaa aaaaattatt aatgatacca atactttata taattcgcga gcgctttctt
    70861 tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt aggaggaatt
    70921 ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg tccgaatcca
    70981 ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat gagtgtagct
    71041 atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa agatatgctg
    71101 atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat ggttgctcgt
    71161 ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc
    71221 ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt aggacctcgt
    71281 cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag tggcaacttg
    71341 ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca agtagcacgt
    71401 atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga
    71461 gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga aaaagaaggt
    71521 tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat tgcttatgac
    71581 ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag tggcaaagca
    71641 ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg cgctgcacca
    71701 aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa ataaatatct
    71761 acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg actaaagtca
    71821 ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct caaatcaccg
    71881 gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg cctttgccag
    71941 ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag gatcgtctta
    72001 ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt gaatgtactt
    72061 tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca cccaaaagtg
    72121 tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc tgaatattgg
    72181 ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca atgtggtatt
    72241 tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc gcatagcaag
    72301 tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat aaatctaaaa
    72361 acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc
    72421 tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc ttcaagaagt
    72481 ccaagaaata aagccgtata aaaacaagaa cctatgaaat ctttatttat tagatattca
    72541 acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa ataaggatgc
    72601 tttttataga atagaactag tgttgttttc atagactctc ctttcttcat atttccaatt
    72661 aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc
    72721 ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa atctaaggta
    72781 cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct tctatctccc
    72841 atccagtata cataaccttg atacttcgac cacttatata tcgaacacac aaatacctat
    72901 ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca gctgaaaaga
    72961 taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca tttttatcct
    73021 caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa atgattaagg
    73081 aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat tagtcatcat
    73141 atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact tggaactaat
    73201 gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt tagcagccca
    73261 accaacatat accctataaa agctaataag atggttagga atcctttaga atttttactc
    73321 attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat aataatattg
    73381 acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac tgtattgata
    73441 gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat tttattaatt
    73501 atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt acattcataa
    73561 ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat tttcgatcat
    73621 tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt tttcagaatc
    73681 tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca ttgtttttct
    73741 tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct ttcctagaca
    73801 gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg acattactca
    73861 tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt tcttctttct
    73921 gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt
    73981 gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt aaaatgctat
    74041 atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta gaatgaacga
    74101 catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat tatagagttt
    74161 acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat gtcttgcaat
    74221 ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt taattgtgta
    74281 ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt cggtaaattc
    74341 tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt aaattcataa
    74401 cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa
    74461 tcaatccata caatatcacc ttttttagga atataaattc tttcttctga cactataaaa
    74521 tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac tcggcctctt
    74581 ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca tcatcagaat
    74641 acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa ggtaaagtta
    74701 ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc actcccttaa
    74761 taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa
    74821 attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt agccagttca
    74881 ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac
    74941 ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga
    75001 aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt
    75061 ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt
    75121 caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa
    75181 ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga
    75241 ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca
    75301 gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag
    75361 agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa
    75421 caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc
    75481 ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt
    75541 tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc
    75601 atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt
    75661 tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca
    75721 gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca
    75781 atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca
    75841 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
    75901 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
    75961 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
    76021 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
    76081 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
    76141 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
    76201 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
    76261 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
    76321 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
    76381 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagctatt
    76441 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
    76501 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
    76561 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
    76621 taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
    76681 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
    76741 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
    76801 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    76861 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
    76921 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
    76981 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
    77041 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
    77101 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
    77161 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
    77221 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
    77281 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
    77341 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
    77401 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
    77461 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
    77521 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
    77581 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
    77641 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
    77701 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
    77761 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
    77821 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
    77881 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
    77941 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
    78001 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
    78061 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
    78121 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
    78181 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
    78241 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
    78301 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
    78361 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
    78421 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
    78481 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
    78541 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
    78601 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
    78661 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
    78721 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
    78781 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
    78841 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
    78901 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
    78961 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
    79021 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
    79081 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
    79141 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
    79201 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
    79261 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
    79321 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
    79381 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
    79441 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
    79501 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
    79561 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
    79621 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
    79681 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
    79741 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
    79801 agttgctttg gggtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
    79861 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
    79921 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
    79981 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
    80041 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
    80101 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
    80161 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
    80221 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
    80281 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
    80341 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
    80401 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
    80461 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
    80521 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
    80581 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
    80641 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
    80701 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
    80761 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
    80821 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
    80881 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
    80941 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
    81001 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
    81061 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
    81121 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
    81181 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
    81241 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
    81301 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
    81361 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
    81421 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
    81481 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
    81541 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
    81601 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
    81661 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
    81721 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
    81781 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
    81841 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
    81901 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
    81961 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
    82021 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
    82081 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
    82141 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
    82201 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
    82261 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
    82321 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
    82381 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
    82441 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
    82501 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
    82561 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
    82621 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
    82681 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
    82741 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
    82801 tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg ttctttcaag
    82861 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
    82921 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
    82981 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
    83041 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
    83101 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
    83161 aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
    83221 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
    83281 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
    83341 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
    83401 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
    83461 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
    83521 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
    83581 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
    83641 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
    83701 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
    83761 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
    83821 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
    83881 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
    83941 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
    84001 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
    84061 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
    84121 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
    84181 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
    84241 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
    84301 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
    84361 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
    84421 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
    84481 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
    84541 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
    84601 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
    84661 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
    84721 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
    84781 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
    84841 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    84901 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgaccctag
    84961 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    85021 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    85081 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    85141 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    85201 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    85261 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    85321 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    85381 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    85441 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    85501 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    85561 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    85621 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    85681 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    85741 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    85801 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    85861 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    85921 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct acggaagatt
    85981 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    86041 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    86101 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    86161 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    86221 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    86281 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    86341 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    86401 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    86461 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    86521 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    86581 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    86641 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    86701 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    86761 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    86821 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    86881 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    86941 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    87001 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    87061 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    87121 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    87181 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    87241 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    87301 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    87361 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    87421 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    87481 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    87541 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    87601 aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    87661 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    87721 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    87781 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    87841 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    87901 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    87961 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    88021 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    88081 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    88141 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    88201 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    88261 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    88321 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    88381 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    88441 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    88501 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    88561 atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat
    88621 tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg
    88681 ttgtataagc aaagtaatcg tttttctatg tttttttatt t
//
LOCUS       DADXTH010000003        75091 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.003, whole genome shotgun sequence.
ACCESSION   DADXTH010000003 DADXTH010000000
VERSION     DADXTH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.003"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(502..1575)
                     /locus_tag="IWI97_000166"
     CDS             complement(502..1575)
                     /locus_tag="IWI97_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2461708.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(1572..2399)
                     /locus_tag="IWI97_000167"
     CDS             complement(1572..2399)
                     /locus_tag="IWI97_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2461709.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(2400..3206)
                     /locus_tag="IWI97_000168"
     CDS             complement(2400..3206)
                     /locus_tag="IWI97_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2461710.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGERKKGGRS"
     gene            complement(3218..4303)
                     /locus_tag="IWI97_000169"
     CDS             complement(3218..4303)
                     /locus_tag="IWI97_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2461711.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(4320..4862)
                     /locus_tag="IWI97_000170"
     CDS             complement(4320..4862)
                     /locus_tag="IWI97_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2461712.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5120..6109
                     /locus_tag="IWI97_000171"
     CDS             5120..6109
                     /locus_tag="IWI97_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBC2461713.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            6106..6843
                     /locus_tag="IWI97_000172"
     CDS             6106..6843
                     /locus_tag="IWI97_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBC2461714.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            6840..7391
                     /gene="coaC"
                     /locus_tag="IWI97_000173"
     CDS             6840..7391
                     /gene="coaC"
                     /locus_tag="IWI97_000173"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBC2461715.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            7407..8039
                     /locus_tag="IWI97_000174"
     CDS             7407..8039
                     /locus_tag="IWI97_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2461716.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            8124..8771
                     /locus_tag="IWI97_000175"
     CDS             8124..8771
                     /locus_tag="IWI97_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2461717.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            8776..9759
                     /locus_tag="IWI97_000176"
     CDS             8776..9759
                     /locus_tag="IWI97_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBC2461718.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(10162..11676)
                     /locus_tag="IWI97_000177"
     CDS             complement(10162..11676)
                     /locus_tag="IWI97_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBC2461719.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            11814..12620
                     /locus_tag="IWI97_000178"
     CDS             11814..12620
                     /locus_tag="IWI97_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461720.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(12795..13364)
                     /locus_tag="IWI97_000179"
     CDS             complement(12795..13364)
                     /locus_tag="IWI97_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2461721.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(13447..15507)
                     /locus_tag="IWI97_000180"
     CDS             complement(13447..15507)
                     /locus_tag="IWI97_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBC2461722.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(15784..17262)
                     /locus_tag="IWI97_000181"
     CDS             complement(15784..17262)
                     /locus_tag="IWI97_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2461723.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(17560..18495)
                     /gene="murB"
                     /locus_tag="IWI97_000182"
     CDS             complement(17560..18495)
                     /gene="murB"
                     /locus_tag="IWI97_000182"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBC2461724.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(18568..19488)
                     /locus_tag="IWI97_000183"
     CDS             complement(18568..19488)
                     /locus_tag="IWI97_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2461725.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            19601..20353
                     /gene="xth"
                     /locus_tag="IWI97_000184"
     CDS             19601..20353
                     /gene="xth"
                     /locus_tag="IWI97_000184"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBC2461726.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            21217..21750
                     /locus_tag="IWI97_000185"
     CDS             21217..21750
                     /locus_tag="IWI97_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBC2461727.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(22240..22764)
                     /locus_tag="IWI97_000186"
     CDS             complement(22240..22764)
                     /locus_tag="IWI97_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2461728.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(22757..23230)
                     /gene="tsaE"
                     /locus_tag="IWI97_000187"
     CDS             complement(22757..23230)
                     /gene="tsaE"
                     /locus_tag="IWI97_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBC2461729.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(23353..24333)
                     /gene="pta"
                     /locus_tag="IWI97_000188"
     CDS             complement(23353..24333)
                     /gene="pta"
                     /locus_tag="IWI97_000188"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBC2461730.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(24486..25166)
                     /locus_tag="IWI97_000189"
     CDS             complement(24486..25166)
                     /locus_tag="IWI97_000189"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBC2461731.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            25292..26161
                     /locus_tag="IWI97_000190"
     CDS             25292..26161
                     /locus_tag="IWI97_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2461732.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            26187..26702
                     /locus_tag="IWI97_000191"
     CDS             26187..26702
                     /locus_tag="IWI97_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461733.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(26749..27342)
                     /locus_tag="IWI97_000192"
     CDS             complement(26749..27342)
                     /locus_tag="IWI97_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2461734.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(27367..27546)
                     /locus_tag="IWI97_000193"
     CDS             complement(27367..27546)
                     /locus_tag="IWI97_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461735.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(27623..28132)
                     /locus_tag="IWI97_000194"
     CDS             complement(27623..28132)
                     /locus_tag="IWI97_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461736.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(28228..28608)
                     /locus_tag="IWI97_000195"
     CDS             complement(28228..28608)
                     /locus_tag="IWI97_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2461737.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            28938..29687
                     /locus_tag="IWI97_000196"
     CDS             28938..29687
                     /locus_tag="IWI97_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBC2461738.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(29871..31148)
                     /locus_tag="IWI97_000197"
     CDS             complement(29871..31148)
                     /locus_tag="IWI97_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461739.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(31227..31871)
                     /gene="pcp"
                     /locus_tag="IWI97_000198"
     CDS             complement(31227..31871)
                     /gene="pcp"
                     /locus_tag="IWI97_000198"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBC2461740.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(32070..33014)
                     /locus_tag="IWI97_000199"
     CDS             complement(32070..33014)
                     /locus_tag="IWI97_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBC2461741.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(33016..33702)
                     /locus_tag="IWI97_000200"
     CDS             complement(33016..33702)
                     /locus_tag="IWI97_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBC2461742.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            34175..34495
                     /locus_tag="IWI97_000201"
     CDS             34175..34495
                     /locus_tag="IWI97_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBC2461743.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            34656..35561
                     /locus_tag="IWI97_000202"
     CDS             34656..35561
                     /locus_tag="IWI97_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2461744.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            35558..36331
                     /locus_tag="IWI97_000203"
     CDS             35558..36331
                     /locus_tag="IWI97_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBC2461745.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(36508..37932)
                     /locus_tag="IWI97_000204"
     CDS             complement(36508..37932)
                     /locus_tag="IWI97_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2461746.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            38143..38589
                     /gene="nrdI"
                     /locus_tag="IWI97_000205"
     CDS             38143..38589
                     /gene="nrdI"
                     /locus_tag="IWI97_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBC2461747.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(38659..40641)
                     /locus_tag="IWI97_000206"
     CDS             complement(38659..40641)
                     /locus_tag="IWI97_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2461748.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGARQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(40655..41551)
                     /gene="murQ"
                     /locus_tag="IWI97_000207"
     CDS             complement(40655..41551)
                     /gene="murQ"
                     /locus_tag="IWI97_000207"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBC2461749.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(41554..42615)
                     /locus_tag="IWI97_000208"
     CDS             complement(41554..42615)
                     /locus_tag="IWI97_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBC2461750.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(42827..43393)
                     /locus_tag="IWI97_000209"
     CDS             complement(42827..43393)
                     /locus_tag="IWI97_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBC2461751.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
     gene            complement(43421..44923)
                     /gene="eat(A)"
                     /locus_tag="IWI97_000210"
     CDS             complement(43421..44923)
                     /gene="eat(A)"
                     /locus_tag="IWI97_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBC2461752.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(45181..46302)
                     /locus_tag="IWI97_000211"
     CDS             complement(45181..46302)
                     /locus_tag="IWI97_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBC2461753.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            46428..47369
                     /locus_tag="IWI97_000212"
     CDS             46428..47369
                     /locus_tag="IWI97_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBC2461754.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(47512..48408)
                     /locus_tag="IWI97_000213"
     CDS             complement(47512..48408)
                     /locus_tag="IWI97_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBC2461755.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            48690..50030
                     /locus_tag="IWI97_000214"
     CDS             48690..50030
                     /locus_tag="IWI97_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2461756.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            50225..50668
                     /locus_tag="IWI97_000215"
     CDS             50225..50668
                     /locus_tag="IWI97_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBC2461757.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(50974..52338)
                     /gene="nhaC"
                     /locus_tag="IWI97_000216"
     CDS             complement(50974..52338)
                     /gene="nhaC"
                     /locus_tag="IWI97_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBC2461758.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAGGS
                     QRNL"
     gene            complement(52742..53965)
                     /locus_tag="IWI97_000217"
     CDS             complement(52742..53965)
                     /locus_tag="IWI97_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBC2461759.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(53997..54836)
                     /locus_tag="IWI97_000218"
     CDS             complement(53997..54836)
                     /locus_tag="IWI97_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBC2461760.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(54853..55533)
                     /locus_tag="IWI97_000219"
     CDS             complement(54853..55533)
                     /locus_tag="IWI97_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2461761.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(55535..56608)
                     /locus_tag="IWI97_000220"
     CDS             complement(55535..56608)
                     /locus_tag="IWI97_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2461762.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(56667..56905)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(56948..57181)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(57258..57929)
                     /locus_tag="IWI97_000221"
     CDS             complement(57258..57929)
                     /locus_tag="IWI97_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBC2461763.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(58017..60695)
                     /gene="mgtA"
                     /locus_tag="IWI97_000222"
     CDS             complement(58017..60695)
                     /gene="mgtA"
                     /locus_tag="IWI97_000222"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBC2461764.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            61355..61792
                     /gene="ndk"
                     /locus_tag="IWI97_000223"
     CDS             61355..61792
                     /gene="ndk"
                     /locus_tag="IWI97_000223"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBC2461765.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(61814..62680)
                     /locus_tag="IWI97_000224"
     CDS             complement(61814..62680)
                     /locus_tag="IWI97_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2461766.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(62914..64992)
                     /locus_tag="IWI97_000225"
     CDS             complement(62914..64992)
                     /locus_tag="IWI97_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBC2461767.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            65485..66702
                     /locus_tag="IWI97_000226"
     CDS             65485..66702
                     /locus_tag="IWI97_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBC2461768.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(67161..67784)
                     /locus_tag="IWI97_000227"
     CDS             complement(67161..67784)
                     /locus_tag="IWI97_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461769.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(67940..69337)
                     /locus_tag="IWI97_000228"
     CDS             complement(67940..69337)
                     /locus_tag="IWI97_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2461770.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(69359..70330)
                     /locus_tag="IWI97_000229"
     CDS             complement(69359..70330)
                     /locus_tag="IWI97_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2461771.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(70337..72637)
                     /locus_tag="IWI97_000230"
     CDS             complement(70337..72637)
                     /locus_tag="IWI97_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HBC2461772.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(72639..73964)
                     /locus_tag="IWI97_000231"
     CDS             complement(72639..73964)
                     /locus_tag="IWI97_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2461773.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            74195..74620
                     /locus_tag="IWI97_000232"
     CDS             74195..74620
                     /locus_tag="IWI97_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461774.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            74682..74879
                     /locus_tag="IWI97_000233"
     CDS             74682..74879
                     /locus_tag="IWI97_000233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461775.1"
                     /translation="MTVKETKWMIDSPDYCTCNNNKNIHTEFNDQGSLSICDNCHKLV
                     KDSFKSFESYPVKNYVKSKRR"
BASE COUNT        21905 a        15576 c        13174 g        24436 t
ORIGIN      
        1 tcctccagtt taatgtgtct aaaagcataa taaactatcg gctgcaatat gtctattcta
       61 tatttgtatt ccaattttta ctttgaaaca caaatagtcg aatatttgcc tcagatatct
      121 gacaaaataa tttatctacc aatttagttt ttatctaata tgatttttat gttttttcat
      181 agctacaaat attgaactgg attcatatat tagttttatt aatagattac agaagggtta
      241 agtatatttg catagtttcc aattacttta tccttctgta actttttcaa tcttagattt
      301 actagcccgt ctcgggccct attttacaat ggttttgtcc acttttctct ggtagaaaaa
      361 cgtggtagaa aaaataaaat aacacaaaaa aagcggcgtt tcatccgctt ttggtagaag
      421 attggaaaac tcagatagaa aaaggacgat ttttagctct ttagaacgtt gataagtccc
      481 cattcttact gagttttgcc attatctcct atacatcttg aattctaaga ataggtcatt
      541 atagatgcct aggtatttcg atcctagatc tttgtagact tcaagatggg agatcgtgtc
      601 atcagatgga tagaattgtt cgtccgacga aatgtctttt ggcaataacg cttttgcttc
      661 ttcatttggt gtagaataac caatatactc tgcattttgt gccgcatttt ccggttctag
      721 cataaagttg atgaaatcat aggcaccttc tttatttttc acggtttttg gcataacgat
      781 attatcaaac caaagattgg atccttctgg tgggatcaca taatgtaaat gttcattttc
      841 ccacatcata tcggcagctt cgccagaaaa tgtcactgcc acggaggctt cttcattgat
      901 catatacatt ttgatctcat ctgccacgat tgctttgaca tttggtgtta aatgattcaa
      961 tttatttgct gcttctgtca gctgtgccat attcttacta ttcaaagaat agcctaaact
     1021 attcaaagaa agccccataa cttctcgtgc accgtcaatc agcatcaaac tgtcttttaa
     1081 ttccggcttc cacaaatcat cccaatgctt aatttgattt gcatcaaaga atttatcgtt
     1141 atatacgatc cctaaagttc cccaaaaata tggaatcgaa tactgattat ctggatcaaa
     1201 atccaagtct aaaaatcgtg gatcaatatt atttaatcct ttgatcttcg aatgatctaa
     1261 tggaagtact agtttttctt tgatcatttt ttggatcata tattcacttg gtatagcgat
     1321 gtcgtagttt gtcccacctt gctggatttt tgtgaacatc gcttcattag aatcaaatgt
     1381 ttcataattc actttgtatc catattcttt ttcaaatttc gtgatcaaac taggatcgat
     1441 ataatctccc cagttataga tggtaagtac ctttgctcca ctcatgccac ttgttttttc
     1501 aagttgacga acaccaagca gcagcaccgc aataatgatg agaataccta aaaacagaga
     1561 ttgtaatttt ttcatcgcag ccgtgccacc ccctcgcgta ctcttctgtt tttcttcagc
     1621 cgttttggac ggttttcttt actaataaag tagtatccaa tgaccaatat catggagaac
     1681 aagaaaacta acgcacttag cgcattgatt tctaaactga ttccttgtct tgcacgagaa
     1741 tagatctcta ctgagagcgt cgtaaagcca tttcctgtga cgaaaaatgt aaccgcaaaa
     1801 tcatcaagag aataggtaaa tgccataaaa tatccagcta tgattcctgg agaaagataa
     1861 ggcaaaatga tatttttgac tacttggaaa ttattagcac caaggtctct agccgcatcg
     1921 accattgaat cgttcatttc ctgcaatttt ggcaagacca tcaagacgac gattggaatg
     1981 ctgaaagcaa tatgggaaag caatacactc tgaaaaccta atccgaatcc tagtagtgta
     2041 aagaaaatca agaaactagc accgataata acatcaggag agactaataa gatgttattc
     2101 atgctcaaca acgctgtccg tgcacgtcgt tttttcgtgt aatagattcc catagcccca
     2161 aatgtcccga tgatcgtggc gatcaatgca gataagaaag ccagcatgaa cgtattcaat
     2221 acgataatga tcaaacgtgt atcttcaaaa acagcttcat aattttccca agtaaatcct
     2281 gtaaagctgt tcatcgttcc tccttcattg aaggaataaa aaatcagata gaagatcggt
     2341 gcatataaga ataaaaagac gatccctaaa taaagatacg accatttaaa ttttttcatt
     2401 tatgagcgac ctcctttttt cctctcgcct gtcaggagca tcacgatgaa catagcaatg
     2461 attaaaatca caccgattgt agaacccatc ccccagtttt gtgtaaccaa gaaatgctgt
     2521 tcgatagccg ttcctaaagt gatcacccga tttccgccaa tcagacgagt cagcatgaat
     2581 aaagaaagag aaggaataaa tactgcttgg acaccacttt tcacaccatt taatgataac
     2641 ggaaaaatca ctcgtcggaa ggtctcaaag ctattcgcgc ctaaatctct actagcactg
     2701 atcaaagaag gattcatctc ttccaatgca ttgaaaattg gcataatcat aaatggaatc
     2761 tcgatatacg ttgccacgaa cagaaaactg aagtctgtga ataatatctg tttcggcccg
     2821 atcccgataa aatcaaagaa atgattcacg ctgccgtgca cactgaaaat cccaataaat
     2881 gcgtaagctt taagcagcag attcacccat gtaggcaaaa taatcagcat cagccataat
     2941 tgcttgtgct tcagttttgt taaaaaataa gcagtcgggt aactgatgat cagcgtgaaa
     3001 aacgtgatca aaaatgcata ccatacagaa ttgatcgtca tagaaagcga tgtccccgac
     3061 gtaaaataag atagataatt attcagcgtg aattgcccat tcatatcaaa aaaagattga
     3121 taaatgatca ttaatacagg cgcgatcacg aataaaccga gccacaaaat ataagggacg
     3181 gaatagatcc tgcgcattgt tttcattctt gtccccccta ttcttcgtag ctgtcaagac
     3241 gtgcatcgaa ttcttcttca gactcgttga aacgcatgac atggatatct tctggctcaa
     3301 aggccaatcc tactttgctt ccttctttag cttttttcgt tgaatggacc atccattcat
     3361 tttgctgttc atcataacag atgatttcat aatggacacc acgaaacagc tgagtatcga
     3421 ccgttacgac tagttttcct ttatctggtg tcgtgatcgt caaatcctct gggcgtaaaa
     3481 caacttcaac tggttcattt agacgcatcc cgccatcgac acattcaaat tgcttaccaa
     3541 caaactcaac tagattatct tcgatcatta caccgtccac gatattgctc tccccaacaa
     3601 aatcagcaac gaaatgattg attggctcat cataaatatc tacgggtgta ccactttgga
     3661 cgattttccc tttattcatg acaaaaatct catcactcat tgccaacgct tcttcttggt
     3721 catgagtaac aaaaataaac gtgattccta atcgttgttg cagatcccgc agttcatact
     3781 gcatatctgt acgtagtttc aaatcaagcg ccgataacgg ttcatctaat agcaatactt
     3841 tcggttcatt tacaattgct cgtgcaatcg ctacacgctg tctttgacca ccagacattt
     3901 cactaatctc ccgtttttcg tatccaggaa gctggaccat acgcaaagca tcttttacct
     3961 tcttttcgat atctgatttg gacattttct tgatcttcaa tccaaaagca acgttatcaa
     4021 aaacattcat atgaggaaaa agtgcataat cttggaagac tgtatttact tgtcgcttat
     4081 tagctggcaa atcattcaac cgtttccctt caaaatagat gtccccttct gtagcttcgg
     4141 tgaatccagc tataatacgt aggatcgttg ttttaccaca gccggaagga ccaagcagtg
     4201 tataaaattt tccttgttcg atctcaaaac ttactttttt taatactgtt tcatcatcat
     4261 agcgtttcac tacttgatca aatgaaatga ttggtttccc cacgttttta ccccctatat
     4321 tacaaatatg attgcgttgc aacaatcaat acttttgttc gttttttgag atgattcctt
     4381 aattgatgtt gttttgtcgc ttgataatag atcgactgtc ctgcttttgc cacatagacc
     4441 gtctctccta agcttaactc taattcccct tctaatacaa aaataaacgt ttcagataat
     4501 gaaggttcaa acgtcttata ttctcctgca ccttcaaaag tcagcaagac aggctccatt
     4561 tctttttcat tcgaatcagc cacgagccat tccaattcat acccgttttc ttcatcgaaa
     4621 taaaccgtat gatcttcttt tgaataaacc acttgcagat tcgctgcttc ttgatgaaaa
     4681 aattcttctg gagtcacacc taagacttca agaatagaaa agaatgtttc cattgatggt
     4741 gaacttaaat ctcgttccaa ctgggaaata tacccttttg acaaatctgt gcgttcgcct
     4801 agttcttctt gtgtcaaatt cttttggatg cgcaaatttc ttaacttctc accaatttcc
     4861 ataatttcct ccgaatcgaa gttttctaat agtaaacttc ctgtttacta ccactcgtat
     4921 tagtatacga aatttattga aaaaggcaac catttgtaaa attttttttg ctgaatattt
     4981 ctttatcttt tggaataaat tctagttgtt tgattcttta tttttcacct aatttttgtc
     5041 taaatttacg gaagaacaag aataaaagag taggctttat ggtatgataa aagaaaattt
     5101 taaaaggagt gtctcacgaa tgaacgcttt tcgatctttt caggtaataa aggaacctgt
     5161 tttccataca gcaatcagtg atctgccatt tactgagttg gctgaaaatg aagtcttggt
     5221 aaaaattgcc tattctgatg tcaattataa agatgcattg gcaatgagcg agtctggtca
     5281 ggtgatacga acatatccga tgactccagg gattgattta tccggaactg tcgtccaaag
     5341 taacaatcca cgtttttcaa aagaagatct tgtacttgct actggttttg ggcttggcgt
     5401 cacccatcca ggcggttata gccaatatca aaaagtacca ggagactggt tagttcctct
     5461 accaaaaaat atgacgctgc gtcaagcgat gatccttggt acagcagggt ttactgctat
     5521 gttgtgtgtg aacgctttaa tccgtcaagg tatgacctcc gataaaaaag ttgtcgttac
     5581 tggtgcttct ggcggtgtag gcagtacagc gatagccctg ttacataaac taggattcca
     5641 gtcaatcatt gccttttcca gaaaagaaga gtctgtcaca tggctaaaaa gtctaggtgc
     5701 tttgcaagtc gtacgacctg atgaatttct gccagagaca acaaaagctt taggaaaaca
     5761 gcaaatcgac tatgtcatcg acacagtagg tggcgagcag ctaagcagtc ttcttccatt
     5821 gatctcttat aacggtgcag tagctttatg tggcaatgct gggggaatca aactaaacgc
     5881 gactgtcttg ccttttattc tgcgaaacat ccagttgatt ggaattgatt ctgtcaatgt
     5941 accaatagat caacgtcttt ctttatggca acagatggcg gatttgcaga ttgcagatga
     6001 actggtcgtc caagagatta ctctggatca attgcctgaa acagcatcta aactacttgc
     6061 tggaacacac caaggaagaa cactagtaaa tgtaggtgac cataaatgaa gattctaatc
     6121 acagctggag gaacgactga aaaaatcgat caagtacgtg ctattacaaa tcattcgaca
     6181 ggacgattag ggcaagcatt agctgattat ttagctgcca atccggatac cacaatcgat
     6241 tacgtgacta cgaaacaagc actgaaaccg aaaagacact ccaatataac gatttatacg
     6301 atcgaatctg cactggactt atttttacaa ttagaagaac taacgaaaaa agaacactac
     6361 gatgcgatta tccacagtat ggcagtcagt gattttactc ctgcttttag tttttcagaa
     6421 gaacagctgg caaaaaacct acccacttct tcaacgcaag aagaactgga caattggttt
     6481 gcggaaaatg aacagacaga taccactgtt tcaaagatct cttctaacac cgagcatctt
     6541 gtgctcgttt tgaagaaaac accaaaaatc atttcttatc tacgtgaatg gcagccaaaa
     6601 gcaaaaatca ttggtttcaa actattagta gatgtaccaa aggaatctct tctggcagtc
     6661 gcaaaaaata gtctgataaa taacaaaact gattttattt tcgcaaatga tttgacggaa
     6721 atccatggcg agacacacca cggctatctc ttatctaaag atggaacagt ggaagaagct
     6781 caaagtaaat ctgagattgc tgcattgatt actgagaaaa ttagactgga ggaatcaaaa
     6841 tgaaaaagat cttactaggc gttactggaa gtatttctgc ttacaaaagc gctgatctta
     6901 caagtcaatt cgtcaaatta ggtgcccagg tagatgtgat tatgtctaaa agcagcacac
     6961 attttatcac gccattaacc ttgcaatctc tgtcgaagcg cgcagtccat acagacgtca
     7021 tgcaagaatc aaatccgcag gtaatcaatc atatcgaact agcaaaacaa gctgacttgt
     7081 ttctcatcgc gcctgctaca gcaaacatta tcggcaagct agcaaacggc attgcagacg
     7141 atcttctttc aacagtcgca atggcgcttc cagtgggaac accaaaattg attgcccctg
     7201 ccatgaacac gaatatgtat caaaacccga tcactcagcg taatctggaa acattgagat
     7261 ctgtcggcta tcaggaaatt gaaccaagag aatctctttt agcttgtgga gattttggaa
     7321 aaggtgcctt agctgatatt cagacgatca tcgaacaggt catgctgact cttaatgaaa
     7381 gaaatgctta gaaaaaagga gtacttatga aaaatacaaa aaactttaca ttaactgcga
     7441 tgtttttagc tattttgatt ttattagctg ttacgccttt aggttttatc cctatcggtc
     7501 caatcaatgc aacaaccatg catattcctg taatcattgc ttcgatcgtt ttaggaccaa
     7561 gactaggtgc ttttcttggc ggtacatttg gactgatcag catgatccgt agcacagtta
     7621 tccaaacacc tttgtctttt gtcttttctc cgtttatccc agtcatcgga acagaacatg
     7681 gaagctggaa agccttgctg atcgcgttta ttcctcgcat cttgattggg gtggttcctt
     7741 atttcgttta taaaggtctg cgcaaactaa tgaaacaaaa agcagattcc gtttctttat
     7801 tcttagctgg actttccggt tcattgacca atacgatttt agtgatgaat atgatctact
     7861 ttttattcca gcaagattat gccaaagtca tcggaacaaa tttaaatgct gtttattcag
     7921 ctattctagc ggttattttt acaagtggta tacctgaagg aatcgtcgca ggtcttgcga
     7981 ctgcagcagt atgcaatgtc ttgttaagat acttcaagca cccttctctt actaactgat
     8041 agtcatcgta gccgatctct gatttttttc tcttttggtg ttactattga atggaaagat
     8101 ttgacgtcga aaggatgatc caaatgaaaa tttttgttgt aggtgcaaac ggacaaattg
     8161 gccgacacct gataaaagat ttagctccaa gttcccatga aatttttgca ggggtgagag
     8221 atgttgcttc ccaaacatta gtaaaaaaag agaatgtttc gtatgtctct tttgatttaa
     8281 cttggtccgt ggaaaaaatg accgaagcat tcaaaggcat cgatgtattg atttttgctg
     8341 ccggctctca aggaaagaat cttctgcaag tagacttgga tggcgcaatc aaaacagtga
     8401 tcgcagctga aaatgcgcat gtctctcgct atctgatggt cagtgctgtt tatgcagatg
     8461 aaccagcaaa atggcctgag tccatgacag attattatat tactaaacat tatgcagatg
     8521 aatggctgaa acgaactaac ttagattttg tcattcttca accagtcaca ttgactaacg
     8581 atgaagaagt tacttccatt caattaacta agcctaatga gaaggcatca aaaacgatca
     8641 ctcgctctac agttgctgct gtacttgccg ctctagtgga agaaacggat atttctcgta
     8701 cgactctagt actttctgaa ggtagcaaag agttgaatac agcattccaa gaatgggcaa
     8761 aggaggaata gatccatgcg tgccatttca atcaagcatc ctggttctgt agatgtatta
     8821 gaacaaattg aagtgcctgt tcctaaacca aaacaaggcg aattactgat caaagtccat
     8881 acagcagctg tcaatcgaac agatattatg aaaagagaat cagtctcttt gaaacctccc
     8941 taccctattt tgggagtcga agtagctgga gaggtagttg agaataaaag tgatatctcc
     9001 acttttactc ctgggacaaa agtatacgga ttagtcaatc taggtggtta tgccgaatat
     9061 gctgtgatgc ctgccgatcg tgcaatcctt ctccctgata cgctagacta tgtttctgct
     9121 gctggaatct cagaagtttt tttgactgct tatcaaacac tttactggct tggaaaatta
     9181 caagaaggcg aaactgtctt gatccatgca ggagcaagtg gtgtgggaac agcagcgatc
     9241 caattagcta gacagctttc tcacgcgaaa atcatcgtta ctgcaggatc agaagaaaaa
     9301 ctcgcctttt gtaaagaact tggtgcagat gaattgatca actataaaaa acaagacttt
     9361 caagaagagg tacgaaaaat cactgaaggt cggggagtcg atgttatttt ggatttcatc
     9421 ggcgcttctt actgggaaaa gaatttagct tctatagccg ttgatggacg ttgggtcttg
     9481 attggtatgc taggtgggac catcgtgcct gagatagacc ttggtgtatt gattgggaaa
     9541 cgtgtccaac tgcttggtac attattgaca cctagaagtg atcagtataa agcagagctc
     9601 gcacaaactt ttatgcaaaa agctgggcct tttctagaaa atggcaagat tcggccaatc
     9661 attgaccgta cattccaatt cagcgcagtc aaacaagcgc atgaatatat ggaagcaaac
     9721 aaaaacatcg gaaaaatcat tttgaagata actgaataaa atcatgctga tcaaaaaaga
     9781 ggctgggaca gaagcgttta actccaagaa ataagaagaa attcacgaaa attgcttttc
     9841 aaatttttgt gaatttcagc ttatttccga aggaatttct tctgcttccg ccgtttatac
     9901 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt tttccgaata
     9961 aacggtgttc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataagaa
    10021 gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca caacctcttc
    10081 actaaacagt gttcaatcag ctgaccctcg gtattagctc aaaacgctga tttcacaacc
    10141 tctttttatt tacgttctat ttcattcatt ttccgaagca aatcgagctg cttgtatgcc
    10201 cgcttgtcgc ccaaaaataa tgatctccgc aactgaattt ccaccgatcc ggttgtttcc
    10261 atgtaagcca cctgtgactt caccagcagc atataacccc ctaattggct ggttttcttt
    10321 atctaatact tcagtgttcg tattgatttt cacaccgccc atcgtgtaat ggattcctgg
    10381 tgcgattttg atcgcataat aattcggaga cgataagtca tgttccatgg ctgtggcacg
    10441 gttgaattga gcatcttttt tctccgcaac atccgtattc catccatcaa ctgtttttgc
    10501 tagtgtttct tttggtaaat cgattttttc tgctaattct tccattgtct ttccagttgt
    10561 gacatatcct ttttggtcat aaaattcaat agctgtggca cgatcacgca cgccttggtc
    10621 aaaaatcaaa taagctgatt tttcaggcaa tgctgtaatt gctgcagata ctttatccct
    10681 tgtatccatt tcattgacaa atcttttccc ctcttgatcg accaagattg ctccttctcc
    10741 gcgaacaacc tcaccaatca agaccccctc atcctgctgc acagttggat gaatttgtat
    10801 tttatccata tcaacggttt gtccacctaa tttttcaatc atggtgatac catctccagt
    10861 tgatccttct tgatttgtcg taatataatc agctaaatcg ggacgatatt tttcaataaa
    10921 ttctttgctg gcaccaaaac cgcctgtggt aactacgatt gcttttccgg tgacctcttt
    10981 tggttcttct ccttgaacat gtacagtcac ttcattgacc gtttccccat ttttcttaat
    11041 gtctgtcaca tctgcgttga caaaaatagg gatttcccgc tcatggacgt tattcaataa
    11101 cccctccact aagtatccac caatagccga gccatcagac ggacgatgtg tcctcttttc
    11161 actcatccct ccagtgatcg ttaagttgtc cagtttgata ccgttttgat ctagccaatc
    11221 gaccgcatca gcagagtgat cgacaaagta tcgaagcatt tctttatcat tcgttcctcc
    11281 accacctttt agcgtttctt catagaaggc atcattcgag tctttgattc cttcttcttt
    11341 ttgaaatttt gtttctgaag cattcatacc gcttgatgat ttagatgtgt ttcctccggc
    11401 aacaggcatc ttttccaaaa ttactggatt caacccagca tcttttgctt cgatagctgc
    11461 tgccatccct gcaccaccag ctccaataat cacgacatca taagattctt tcatttcttt
    11521 tggatctgta tatacttgtt cagaagcgcc tgatgatact tcggcagatt ccttcgtact
    11581 agaacttgtc tgcgctgtat ttttctgatc tgcgccgcat gcgctcatag caagaagtgc
    11641 aacggctgtt aataaaccag caactaattt tttcatttgt atcccccttt tctccttata
    11701 ctatattaag tgattttttt cacaatatca actctgtttt tctttaagtc atatagcttt
    11761 ggtaaacaca cgcttcttag tataattaag gatatacatg aaggagttat tttatgaatc
    11821 atttgaaaac cttttttacg aacagtacaa aaactgcagg tagaatcaca aacggaatct
    11881 atattttatt acgtatcatc gtcgttatta tgctagtgga tcaggtctgg aaccaacact
    11941 ggggaaatac aatgctttta ttactgacat taattttatt tgaaatccct tattttctgg
    12001 aaaagatatt aaagatacag attccaaata tgctagaaat cattttgtta tcttttattt
    12061 ttagttcaac agttttagga gagttaagtg atttttatgg ctatttttca atgtgggata
    12121 cttttctaca tgcattcagc ggatttttag ctgggggtgt cggcttttcc cttgtttatt
    12181 tgctcaataa aaatacaaaa accatgaact tgactccttt attactagca gttgtttcct
    12241 tctgtttttc gatgacagtt ggtgttatgt gggagtttgt ggaattttca gcagataaat
    12301 tactgcaatt cgatatgcaa aaagatacga tcatccagca aatcaatagt gtggagttaa
    12361 gtaaagacgg aaacactgtc tatcatgttc cagatatcac tcggacaatc attgaaagtc
    12421 aaaatgcaga tggtaaaaca gataaaacaa tcatccaaaa tggttactta gacgtgggaa
    12481 tcatggatac tatgaaggat ttatttgtta attttattgg agcagttgtt ttcagtgtat
    12541 ttggttacct ctatagtcga aacgattacc aaaaatttac gttcatcaag aattttattc
    12601 ctaaaaaaat caaacgataa aacatgctaa aaaacaagag aaggggcagt gacaatactt
    12661 ttgtcactgc cccttctttt ttgaatgtat ggcatgccat tagttatccg atagtattaa
    12721 ctcaaaaaag taagatatga ggaatgacta tttcgttttt tatcccaata ctgaggactt
    12781 atttcaccat ttcgttattt atctaattta cttaaaacct gcataacttt ttcataatct
    12841 ggattaggtg tgccgtctag attttcagca ataaaagtaa aacccatttt tttataaaga
    12901 gcaatcgcct gatggctcca tgtctgtgtg tgaagataga ttggtccgtc cgtatggagg
    12961 gtaggaaatt cttgcaagac tctggcagtc aatgcttttg cgagcccttt tccttggtaa
    13021 tctggtttca ctgctaacca atgaaaaacc ggatacactt gctctcttga tttttttcgc
    13081 caagcggtac aggttgctac tttttctcct ttttttgtct ctacaaaaaa catccttctt
    13141 tcaagttctt caggataagg agcaaagttt tgttgaaaat actcaagtgc ttcatctgtc
    13201 gtttgaaatt ccccaacgga agtctcaatc tccgcccaag cagattcatc ttctttttga
    13261 taaaatcgaa attcaaaacc ttccgctaaa ggcgtgattg gaacattttg ttgaagtggt
    13321 cttgtcatcc agatttctgc gtaaggtaac gttttatcta acatttttct atccctcctc
    13381 gtctccatta tgccaaataa catatgactc agctacttat ttgactataa cagatagaga
    13441 tataaatcag aaccagtcat ctgcgtcaaa ttcatttcga ttagcgtata cccatgttcc
    13501 tgccatacct cgcatgaaat ctcctaaagc agaagaagga atacctccac caggtgcggg
    13561 tgttcccgga ctaggaagtg gtacttgcgg ctgcagatgc tcatcggtgg aatcaatagt
    13621 tgacaagcgt tttttcttcg tttccttctg gtcatctgat tccttctgat cttccgactg
    13681 tttagcaaca aattcttttt ctgattgttg ttttatttta ggctctaaaa tttcattttc
    13741 caatccagaa acttgtgggg ccaaatgagc ttcttgcctt atcgttttat tagattctga
    13801 gatttttcta gatattcttg ctaactctag gcttcttgtt tgttttactt tccctgttgg
    13861 agaaactgca tgggccattt gttgtttttt ttctgacaag ctttcttctt gtcttttttg
    13921 ttctttttga tctatctgtt tgtttaatgg taaaacatgt aatagtgact gagcatggag
    13981 ttcttggagt gaatacgggt catgataact atacataatc gtaaaatcac ctgtttgttt
    14041 ggtcaaataa ccatcctcca atggtacttc attgattgta accgatacat cctttaacgg
    14101 tacgggttta cctaaggaat ttgttgctga ttggatcaac tcttctttgt ttattttttc
    14161 gtttggatga acaataagat catgaccttc taatgaacga ttgtctggta tataaacata
    14221 agtaaaggta atttcccctc ctgattgctt cctattattc atcagttctg agtgaagtac
    14281 ttcaaggatc tccgaccagt gactttttct gtctaatccg tttgcttgca tccaatcaga
    14341 aatagaagtg actttcccat tttgttcata agtcgataaa tctatatttg gcactcgatc
    14401 agaaaattca gcaggtaggc gcaaatcagg aatacgattt acactactca tatgtgtttc
    14461 tgcttgaagt tctttatttg tttgactatc tataaaatga gtgatgacag tcgatgaagc
    14521 agcagatgct tctacataaa ctacagctgt tgtttctgcc acatttgatg ttttcgagtc
    14581 tatataagtt aatacaacgg gatattctcc cggagttgta gtatctactg tcccgttcat
    14641 tttgatttct tctttatttc ctgaagttcc atcgcttttt gttacatatt caacaagatc
    14701 tttaggggcg taactctctc ctgttcttaa gtgaagatct tttcctttga tagctgtttg
    14761 atcagcacga acatggatca cagcagatgc agtcattgtt tttaaagcaa ttggatcaaa
    14821 ataggacaag gaaatagtag atgttcctgt attagtcgta tccacgatat tcccattgtt
    14881 tgaaattact ttggacatat ctccttgtgt tccatctgcg ttcttcagcc tttctaccag
    14941 atcagatatc gaaacttttg tccctgccag aacatcgatt ttctttcttt caaactgtga
    15001 ataatctttt tcatatacat aagaaacttg tgcgcttcca ccgtccttta tatctgtcaa
    15061 agcatgattc aaaatatcag tgatctgttt taactgatca ccttttgtag gatcagtcat
    15121 atcggaaaag atagaaagcg gcacactgct tccggtttcc gatgtccata atgttttgtt
    15181 aggaattaac cagtagtggt ttatcgttgg aggataactg actttattat aggaagtcaa
    15241 tgcaggaagt agctgttttc catttgcatc agtgaaagta tgagtaatag tgatagggga
    15301 atcagtggtc tcagaaacag aggtacgtac gaccttgatg gtttgtgtcg ttgcctgaac
    15361 gggtacatgc atatagacac ctacagataa actgatcagc atcgtgcgca tcataaactt
    15421 aacgtagtgt ttttcagaac acgaccgttc cttatctaaa cgacaagcgt gtttatttag
    15481 ttgttttgaa cgtctttttg aacacattta ttcttagtcc tccctactct tgtctaaaca
    15541 tgaagtagta attttcataa gatgatttca ttatataaat aaaatcaaaa gaagatggac
    15601 tattaggaga gtattttttt tgatttttta ttgtttcaag aaaatcggaa taataactta
    15661 tgtacttttt aattttgtta agtaatatat ctagttatat ctagttatat ctagttacct
    15721 tttctttgaa aaaaacataa atcagctaat aaatatgtga gacaaagctg ccatccataa
    15781 atactagctg attttttcag ttaaatgtct atttttttct ctgatgatgg tatttccaaa
    15841 atattcccag tcatctggag tcggtggatt tttccaaatt atctgttctt tattaaattt
    15901 ttcaagtaaa caatcagata aataaatatt cgtacgagaa gtgattcgac tctcaatcac
    15961 aatattgtaa tttttaaatc ctttcacttg agaaataatg taatcggtat aagcagatcc
    16021 ttgcgaaaaa tcaatacaga catgaatcgg tgcttcaaaa tatgataccg gacaaatacc
    16081 gaccaataaa aagagataat cataaaataa gcgaatcatg aaacgttcat cttttcccaa
    16141 gaaatagcgt ttattctctt gtatgacctt ttggattgct gtactgaata ttggatacgt
    16201 ctcgaagaaa aactgtagct gctttttaga cgtaaagcta gtcatctcaa agtcgaatat
    16261 ccaatttttt cgattgacat gcatcatacc catacgaagt tcttttcgta tatatagtct
    16321 ttcttcttca gagatttcaa tcgaaaatgg taattgatca atcaattcca ctgacatttt
    16381 ttgactaatc atgtggatat catgaaaatg agcttccgca agtccaaatg cgccatctaa
    16441 ttcaaattct tcttccgtga tcaaaaacaa catgatataa gaaatctcat caatgatctg
    16501 ctctttaggg atatgaagaa aagaaatatg tgataaatcc ttgatgatct tcattccttc
    16561 tgagctctta aagtattcat ctgaaaagaa acgttttccg attggttgat tcccataggc
    16621 cctcaacaaa ctaatagtgg ttagcaaatt gaatttgatt cgttttgttg cagacagttt
    16681 taaattgaac agatattcaa aatattgcac gatactcttt gcttcattca tgacgctttc
    16741 tctaaatggc atagaaaaac cattaaagac cccgaaatat accccaaata atacgttccg
    16801 tatgtttatt tcttccccaa ctaatatgtt tttctttaac tttatttgat gctgttcaag
    16861 aaaagagact aattctcttc tttttacata ggctcttgat atgcttaaaa aatgattctc
    16921 tgcaaatttt tcgatcgtta cttttgaatc cacgatctcc gttaacagtc gagcaatttt
    16981 tgatcgttga aagtagattc ttcttaaatt tttgatatcc gtcaaattca gacgttcgac
    17041 atggatctct gcttcttctg aaatttggat ctctggcttt gttttgaatt cagtaaattc
    17101 tttatttaat tctatgatat aatttcttgt tttaaattta gacaaaccta atgtttcata
    17161 taacttaggc agcactacat aattcttttt ctcaaattct aaatatctag ctagcagcag
    17221 tttttctcta tcttcctctt ctataaggta atccaacttc atatcctaca cctcaaaaaa
    17281 tgcctcatac caattacttg atatgcagat gaaagttcat cttttcacct atactgattt
    17341 tatgatggat tatgcttgtt ttttcatcag ttattctcat tttttatctt ttttttgaga
    17401 atattgcaaa aaatggaaaa atttatgagg aaatattcta tattttttgt tttattgtgt
    17461 taaaatgtac aagatttgcg ccatttgcgt aaacaaccac aaaaaagagc aggacggcca
    17521 ccgtcctgct cttttactta ttttaagttc cattacttat taattggcta aacttctgct
    17581 tgtttctgat attgcttgtt tttctccaat gatccgcact tctgtttcta aagacacatc
    17641 aaatttttct ttgattactt cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt
    17701 cgcatgatcg atattgacga tgaatccagc atgtttttca gagacttgtg caccgcccca
    17761 tttcaggcct tgcaatcctg cttgttggat cagctgacct gtatagtgtc ctactgggcg
    17821 cttaaagaca cttccgcatg aagggtattc taatggctgt tttgattgac gcaattcggt
    17881 caactcatcc attcttgctt tgattgcttc ataatcccct gtttgcaaac tgaatcgagc
    17941 ttccaatacg atagcacgta attcttggac tttactgtga cgataagaaa ataccatttc
    18001 gtcttttgtc aaagttttca atgttccatc ttcaagcaaa agatccactt cagcaaaaac
    18061 atctttgatc tctccgccat acgcacctgc attcatataa accgctccac cgacacttcc
    18121 cgggatgccg caagcaaatt caaatccagt caatgattct gccaaagctg cataagtcgt
    18181 atcaatcagt gaggcacctg cttccgcaat gaccatcgtt cctttgacat gaatctgttt
    18241 catttcagtc agcatgatga ctacaccgcg aatcccgcca tcttgaacga tcagattgct
    18301 tgcatttcct aataccatcc aaggtatatc atggattcgg caataatcga tgatttgctt
    18361 tacttcttct tgttttttcg ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa
    18421 cgtatagttc atcaatggtt catcaaacaa tatagtaatc gtggctaagc tttcaactat
    18481 ttcttcttta ttcactgagg attttccttt cttttctcaa acgtcctcat tattctatca
    18541 cgcttaatac aatcttgcca gtcttgttca aagttgatga cggtagtgat ctttaagcac
    18601 ttcctcacat aattcatgac ttcttacaac ttctttttgt ttaagcaatt ctgtttccga
    18661 tttacctaaa cggatctgag aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac
    18721 tagcgtgttt gtccagtcgc caaattcttt cacttttttt ccatcagcat cttgaatgac
    18781 aagctgggtc agatcgttta cttcataagt accattttta cttgttactt gatatttttc
    18841 tatattagcg ccacttgcta aatccatcgt taaaatagcc gtttgatttt gtgtttctaa
    18901 ttgtaaaatg gcatattcca aaaattcctt tgtttctctt atctggtatt ttgttctcaa
    18961 aatgggttca tctaataaat acacagctgt atcaataaga tgtaaaaaca aatcataaag
    19021 gacgaactct gtcgtttctt ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt
    19081 atctgggata cgtttcaatt cttctaccat aggagcaaag cgccggttga aaccgatcat
    19141 caaaattatt tggttcttat cagctaattc ttgcaattca cgaacttcat ttagatggac
    19201 actcagtggt ttgtcaatat aaacatggat accattttct agacattttt tggctaattc
    19261 ataatgtact tttgttgctg cgtggatcat acatgcttcg attttttctt ctaaaagctc
    19321 atcgatcgtt tcatatagat gttggaaccc gtactctttt tgaattcttt cttttgtttc
    19381 tttatttctt gttgcaaaat aaaaatcaag tgttccttgt tttttcgcat aagttggtaa
    19441 atatgctttt tctgcgatat tccctacacc gattacacca atcttcatct actttcctcc
    19501 tggatcattt agttactttc atcttacacc attttttact aaaatgaggt acaatagtat
    19561 cgcaaaggta aggaaattaa ttcgaaggag tgtgctttta ttgaaattta tctcttggaa
    19621 cgtaaacggg cttcgtgcca tcgtaaataa aaatttttta gaagtttttc atgagttaga
    19681 cgccgatttt ttctgtctgc aggaaacgaa actccaagca ggacaaattg atttagatct
    19741 tccaggttat tatcaatatt ggaattacgc ggaacgaaaa ggctattccg gcacagcgat
    19801 ttttgctaaa aaaccagcat tgaatgccac ttacggaatg ggtatcgata tccatgatac
    19861 agaaggccgt ctgattactt tggaatattc cgatttcttt cttgtaacct gttacacgcc
    19921 aaattctcaa agcgaattaa aacgtttaga ttaccgatta gaatgggaag aagcctttta
    19981 taattatttg gaaaatctaa aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt
    20041 cgctcatcaa aagattgatt tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac
    20101 tccagaagaa agagcggctc tttctcgact gttggataac gggtttatcg atacattccg
    20161 ttatttttat ccaacccagg aaggtgtcta ctcttggtgg aactaccgat tcaattcaag
    20221 gaaaaacaat gctggttggc ggatcgatta ctttttgacg agcaaagatt tagagccagg
    20281 actagctgat gcaaagatcc atacggacat tatgggcagt gatcattgtc cagtagaatt
    20341 agatttgaaa taaagaggtt gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt
    20401 gtgaaaatgg ctttccacat tttgcataaa tttggcataa taccgagaaa ctggcttttg
    20461 aacactgttt atcgagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
    20521 aacaaaaata gttcctcata tttttcgttt ggttgggctt attaccgcag atacaagctt
    20581 ttgaacaccg tttattaaga ggttgtgaaa aagctgcact gcatcaagta ataagaacag
    20641 ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg cagatgcaag
    20701 cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa gtaataagaa
    20761 cagccaaaca aaaatagttc ctcatatttt tcgtttggtt ggacttatta ccgcagatgc
    20821 aagcttttga acaccgttta ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag
    20881 agaaacaaat ggcaaaatag ttcctcatat tttgctattt tttgagctaa taccgagggt
    20941 cagctgattg aacaccgttt agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt
    21001 aagaaatttt tttgaaaatg gttcctcaca actttgcata aatttgactt aataccgaga
    21061 agtcatgatc cccatctaac tccaaatcac aaggaatgag ttcaaaaact agcttttctc
    21121 aacaaatatt acataaaaaa ttctatgttt tttgttaaat ttatttgctt ttttcaaaag
    21181 tatgttacag taatttccca aggaggtttt acagaaatga attttgttgc catggatttt
    21241 gaaactgcca atcatcagcc ttatagtgct tgttctcttg cgctagtcat ggtaaaaaat
    21301 agccagatcg ttgatgaatt ctatacattg atacagcctg agactccttt tttctggcga
    21361 aatgtccaaa ttcacggaat ccatcaagaa gacgtacgca atgcgccgaa attcccagaa
    21421 gtctggcaaa ctatccaacc atattttcaa aaaaatcgtt tagtcgttgc acataatgct
    21481 gcttttgata caaaggtctt agcgggatgc ttagattatt atcatcttga acagcctaat
    21541 tatctttctt tatgtacagt gaaaagtagc cgtcgcctgt ttcctgaatt tccaaatcat
    21601 cgattgaaca ctgtgtgcga aaatttgaac atccaattag atcatcacca tgatgcattg
    21661 gaagatagtc gtgcatgtgc ggaaatcctg ctttatcaag aaaaaaaatt cgggacagat
    21721 ccattgaaaa aacttgtgac tataaagtaa aagaatactt atgaaacaca caaagaggtc
    21781 gtgagcctgc cgtttcactc cgagtcacaa ggagcaattt caaaaaatag cttctcatgt
    21841 ttattaaata ttgtgactgg tgaccgagga gctggcagga gaacaccgtt tattggagag
    21901 gttgtgaaaa agctgttctg catcaagtaa taagaacagc caaacaaaaa tagcttctca
    21961 tatttttcat ttggttggac ttattaccgc agatgcaagc ttttggacac cgtttaccaa
    22021 gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaacggca aaatagttcc
    22081 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttattc
    22141 aaaaaaaatg gactgcgaca cgacttttgt cacagtccct tcgtgtgttt ttctgccttc
    22201 tcaaaaggat gacagtttct cactcttttc ctatttcttc tattcaatca aataactcat
    22261 tttatataca tctaaaaatt cacctagatc tgttcttgcc cctctaggca tgaccgcttc
    22321 gatttggaag cccatttttc ggtaaaggtg tacggctctt tcattacgaa cttgaacgtc
    22381 aagctccaat cgaaaaagta cacccatttc cttcgcccaa ctgatgattt cttctagcat
    22441 aagagtgcct agtcccattc cccaatattc ctgcagaata ctgataccga cttctcccac
    22501 gtgagacaaa cgaaattgac tgtctgcctt gacagatgct gttccaataa tcgtcccttc
    22561 atagattgca agcagcaata aattattatt gctttcgtat atataatcca actcttcttc
    22621 taaggtttct ggtggtaaca ggagttctgc ttcatccaaa actaaaaatt ctgtctcctt
    22681 attaacttgt cccatcatca aaagaatgtc ttttgcatct cctggcactg cttcccgcaa
    22741 gatgatccca acttcttcac tcatgatttt cagcccattt ttctagaatt cgctgtttga
    22801 aggtttcact ttgctccccg tatgagatta gtttcacata ccgcttttct aaatctgtat
    22861 cacttttttc taaaatcagt tccaaataat cctccggcag tgcttcttct aatagatttc
    22921 cccattcaac aacggaaagc ccatcacctt caaaatattc atctaaccct aaatctgctc
    22981 cgctagctgc tacccgataa atatccatat gatacaacgg aatccgacct tgactatatt
    23041 ctctaataat cgtgtatgtc ggacttttga tcatttgcgt tatccctaat ccttgggcaa
    23101 ttcctttcgt caacgttgtt ttacccgcac ctagatcacc agtcaatatc agattgtcac
    23161 ctggcattgc ggaatgacca attgcctctg cgaattgttc tgtcattgtt aatcctgata
    23221 gttcgatcat ttttcatctt ctcccgctct tctttctttt tgccagtata tcgcgtaatg
    23281 aagttttttt aaagaaaaaa tgaactagtc aagaacagca gctgctgttt ttggctagtt
    23341 cgcgtgtatt tgttaatcaa tcaatgtttg tgctgctgtg ataatcgata atttatatac
    23401 atcttcttca tttgctccac gagacaaatc agaaaccggt ttgttcaagc cttgtagaat
    23461 tggtccgatt gcttcaaagt taccgaaacg ctgagcaatt ttatagccga tatttcctga
    23521 ctgcaattct ggaaatacaa agactgttgc ttgaccagct acttctgaat ctggcgcttt
    23581 cagttgtgca actgaaggaa cataagaagc atcaaattgc aattcgccgt cgatcatgta
    23641 ttgtggcgct agttttttag cgattttagt tgcttcaact actttgtcta cttctggcga
    23701 tttcgctgag ccttttgtcg agaagctcat cattgctact tttggttcga tatcgaacaa
    23761 ttctgctgtt tttgcagaat ctacggcgat ttcagccaat tcttgcgcag ttggattcac
    23821 gttgatcgca caatccgaaa agacatattt ttcttgatct cttccacgta ccatgatcat
    23881 cgctccgctt gtccgactga tgccaggctt tgttttgatg atctgaagtg ctgggcgaac
    23941 agtgtcgcca gtcgaatgga tcgctccaga gaccattccg tctgcaatcc ccatatacgt
    24001 aagcatcgta ccaaagtaat tcacatcttt taaaatcttt tctgcttgtt ctttggatgc
    24061 ctttccattt ctacgttcaa caaaagtatc caccatttca tcccacttat cgtaattatc
    24121 agggctgatg atcgtaaagc tagatgtttt gattcctctg gcatgcgctg catttcttat
    24181 ttcttcttcg ttccctacta aaatgggttc cacaagttct tctgctttca accgggcagc
    24241 tgcgcctaaa atacgagcat cagtagcttc cgggaaaacg attttgatcc cacggcgaac
    24301 aattttaaat cttaaactat caaacaattc cacaagatga cctccagacg tttttatatg
    24361 tattatcata cactattcca gaaaaaaaga acagtttatt ttatctgtat cagtcaaaaa
    24421 tgctggaaaa attaagtttt tcacatacaa agcgaactgc actataacag gaaagcgctg
    24481 ttttattaca ctgtttcagg cagttgccaa tcaatcggtt cttctcctaa cgcaaggagc
    24541 gcatcattcg ttttcgagaa gggtttggaa ccaaaaaagc cacgacttgc agataacgga
    24601 ctgggatgtg gagatgttat gatgatatgg cgactcttat cgatcatttt gattttttct
    24661 tgtgccggtt ttccccacaa gataaacacg attggttttt cccgttcatt taatttttca
    24721 atgatcgtgt ctgttaatct ttcccaacct ttcccgcgat gagaatacgc ttgcccttcc
    24781 cgaacggtaa gaacggtgtt gagtaaaagg actccctgtt tagcccatga cactagattt
    24841 ccatgttgaa caggtgcgat tccaagatca tcataaagct ctttatagat atttcttaac
    24901 gatggcggga tctttacgcc aggttgcaca gaaaaagata agccatgagc ttgattttct
    24961 ccatgataag gatcttgtcc taaaatcacc actttaactt cttcataagg agtcaattcc
    25021 aatgcttcat agatatgata catatctgga tggatctttt gggacgcata ttcttttttt
    25081 aaaaattctc ttagatgcag ataataatct tttgaaaatt ctgaggacaa aacttcttgc
    25141 caactgttat gaatgatttc tttcaaaaac tccacctcct aataaaaaga ataactcaca
    25201 ttttcttcaa tcacaaggga caatcttcac cctcagcact tgattaatat ggtaaactag
    25261 gaacaagaag cagaaaggaa cgataatcat tatgatcaaa ttaattgcat cagatatgga
    25321 cggcacatta ttagatgccc atatgagtgt ttctcctgaa aatgccgagg cgatacgttt
    25381 tgcaaatgat gccggtgttg agttcatggt cgctactggt cgaaactatc aagaagctcg
    25441 cgcagcttta gatgaggtag gaatcgattg tgcgatgatc actttgaacg gggcacaggt
    25501 atttgataaa gaaggaaatt cattgtttac cgtgtcgatt ccaaataatc aagcaatctc
    25561 tgttttagat attctagatg ctaacggtat ttactatgaa gtagcaacca acgatggtct
    25621 ttattctgaa aatcaagcga aacgaattga aagttttgct tccatggtgg ctacccattt
    25681 acctcacctt acgtataaaa tggcgattgc catggcctca gctaagctgg agttattgca
    25741 catcacatac gtggacagta ttcgtgctat tttggaagaa aagaatctgg aagttttaaa
    25801 aattatttgt tttcatacag aagggccagc tgtattaggt ccagttggaa agcagatttc
    25861 tcttttaggc gatctagccg ttacttcctc tggtcaaaac aatatcgaag tgaaccataa
    25921 aaacgctcaa aaaggaattg ctgttgccca tgtcgctcac gagcgtggga tttctttaga
    25981 agaagtcatg acgattggcg ataacttcaa tgatgtcagc atgctgcaag tagcaggagt
    26041 cagtttcgca atgggaaatg cagaaatcga agtaaaagat tatgccaaat atacaacgga
    26101 cacaaacctt gaatctggcg taggaaaagc catattgcgc gcgattgcag aagacttgta
    26161 gttttatcta gaaaagaggg atatagatgt cagaattttt cattcaggaa aagcagcttg
    26221 gacgtgcaac tcggacaatt gtcaaaaacg aaaaaggaca ttccctgttt ttgatggttg
    26281 gtcgctgggg aacaaagggc gatgcattgt ctctttatgc catgaatggc gatctcgttg
    26341 ctcacatcaa gcagataagc ctgacattcg gtacgcgttt tgagctgtat aaagagttcg
    26401 aaaaggtggg aaccatgcag aaaatattca attggcccgg tgacttttat tatattcgta
    26461 gacttcactg gacagtccaa ggtgatatct ataatcatca atatcaaatc catcatttca
    26521 atcagcaaat catgaaaatg gataaagcaa cacttttttc aggagattac tatgtattgg
    26581 atgtctcaaa tgatgaagat gctccagttt gtatttgtat tgctgcggtt ttagattact
    26641 ggctctaccg taaagacaaa agtcgcgata agcctgtttc ttaccgactt aacttaagtt
    26701 aaaaagatgt acctgaaatg cggaaatacg catttcaggt acatcttttt agatattctt
    26761 ttgcatatga tcataaagat gaccactgat catccgctga ccgacaactt tgaagtcatg
    26821 gcgttcgtat aattttttag cctttggatt tttttcatcc acacttagac cgatgacgga
    26881 gcgattagct ttcttcgcca atttaggtaa tgcttctaat aatcgcgaac cgatgccttg
    26941 tcctctaaaa tcttctgata cactgataga gtccaaatac cattcgttgg gaaacgtttc
    27001 tttatcaatg aacaaacgtg tagacgcatc tagctgatat ttctctaaga taggtaccag
    27061 tggctgatcg atgattgatt cttcttcatc tgtataaccg taagcaactc ctgcaacttt
    27121 accgtctatt tcttctacga tacctcgtga ataactgtaa cgataatttg gatcagtgat
    27181 tgcttcttct aaaatctcta aagtcttttg ttcccctgtc tgcttcaaaa atggcagttc
    27241 catatctttt agaatcacta agatcaatgg ggcgatttct ggagcatctt cttttctcgc
    27301 aaaacgaatc atagtttcct ctccttttct ccattctaac atgcagaaaa agaaatcgca
    27361 aaatcgttat tttaaaaaat cttcaatctt ttcagtaatg aaataaccat gagtaacgat
    27421 tcttgccgct tcagttcggc aataagggca gacgactggc atttctttat cattatgttc
    27481 gactacatta ttgacttctt cgtatgcaaa tttttttccg caattagggc atttcatttt
    27541 ttccatgtta tctcaacttc ctttcatctt tactctctta gcttactctt atattgtaaa
    27601 aaagagaagc aatatccttc ttctatgcag atatccatac ctgacggtcc tgttctccat
    27661 ctattaatcg gactttataa taaggacgtt catctttgac tagaagtgtc cactctttca
    27721 cttttgcttt tttcccgtag tagctgactg cttccttcat cgctgcttgc ggttgtttga
    27781 actcttccgt tttttctata tcaaaaagat gttctgcggc cttttccgtg tcatttcgta
    27841 cggttgtttt cccagtggat ggattgactg ctatgacttg tccttcacaa aaaaactggt
    27901 attcatatgt tgcattcgta ggattctcca aatctttttc aactgcaaaa tgaatagcag
    27961 atatgttatg ttcttttgtt tcgtttttta cgattgctgc tgcttttttc aatgagatcc
    28021 gatattcctg gtcactaata tcatccatgt gaaaagcggc attgagcagt cccgtcatac
    28081 cggtaatccc tattacgatg agggcagcaa caccaaacat catttttttc attcttcatt
    28141 ctcctgtttc ttttttttaa agaatatcaa gaaatgaacg aatgaaaaat cgaatgaaac
    28201 gaaaaaaaag tgacaatttt gttatcttta ttcgtgcagt tctaatggca atccgtctgg
    28261 atcaaagaaa aacgtcattt tttttcctgt aaaggtatct tctctaatcg gttctgattc
    28321 gatccctaag gactgcaatt ctgcaattgt ttcttcaata tcgtctactt tgaaagcaag
    28381 atgacgcaag ccacaagctt ctggatagtt tggtcgttgt ggcgcattag caattccaaa
    28441 aatctccaat tcagcacttc ctagtttcag atctaattta tagtcatctc gttctttgcg
    28501 gtaattttct ctgattactt caaatcctaa tttattaaca taaaaatcac gtgatttctg
    28561 ataatctgaa acaatgatgg ctatatgatg gatcgttgta aggttcatat gagcactcct
    28621 attcttcgtt taagctcgga tctatattaa tctccagttc cattgtaaat gtgtcagatt
    28681 gaaaaaaata gctgtttttc ctttttataa gaaaaaaggc aaaaaaaaca gaaagtaggt
    28741 cctccaagac actcttcctg ttcccaatat aataaatgaa agtaagaaat tatattttca
    28801 agaaaacaaa cagaaaatta tcataacaca aattatttat aattgaaaga ctagtttttt
    28861 ttcaagaaaa gcatacaata gattgtgtta gtaaatacag aacacactac atttagtgtc
    28921 ttaaggagtc ctcatacatg aagtcatatt ttcagttttt agctcatcag ctaaaaggct
    28981 ggccgcaaca aaattattat cttttctttt tcagtcttgg ctgccaggtc atgactcttg
    29041 tcaatagtcc catcacttgg ctgtccgtta ttacatttat cggtaccacg cttggtgtat
    29101 tgtgtatttt atccatcaat gcggcaaaat cagtcaacgg tattttaggt attttgtctg
    29161 cgatttgttt tattatcgtt gggttttcag caaagaacta tttaagtatc ggtgaacaga
    29221 ttgcttatat gatcacattg gatatccccg tattgttaag taaagaatgg aatcataata
    29281 tggcttcaaa aatccgaaaa ttaactggaa aaagctggtt tatcgcaatc atcagtactt
    29341 tccttgtcta tcttgtttca ggattcctga ttggtcgtta tacagatgat cctcggccgt
    29401 gggtagatgc cattgctttt tctattagct tgactgctgg tatcatcagt ttcttgcgtt
    29461 acaacaacca atattactgg tggttagctt cagggcttgc tcaaatggtc ctttggtata
    29521 tttcatttac gcatggatct gcaagtttgg cgatgttcgt gaacagctct gtttacttgc
    29581 tgaatgatgt ccttgcattt accgtttcac cttggtataa ccaaaaagaa cgtgaacgta
    29641 tgcaaaaaat ggaagaagaa tactacaacg cacaacaaca cgcataaaaa aactgagcgt
    29701 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaatca
    29761 gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
    29821 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctatttt ttatagtaat
    29881 tccttcagat actttcttgc ctcatcattg aaacgcagtg attcttctaa cttctgataa
    29941 tattccaacg cttgtttctg agcttcagta aaacgataat gttttccttt ccatttgtcg
    30001 attgtctctt gcacatttcc agattggata tctttcacta ctagttgcca tttttctttt
    30061 gagactgtac ggtctttctt atcagcagat tgtatagctt ctcgttcttt acgcaatact
    30121 tttaaaaaac catttctgac tatccgagga atgctgtcga agaacccttc tgtaaaacca
    30181 tagcgacaat attgcttcca aataaataga tagatttttg gttggatgaa tcgttggttt
    30241 ttcgcaaaat attccatctg agcagtgact tctggtgttc gttgacgttt tgtctttctc
    30301 aacacggaaa taaatgtttc aaaataagca ttaggatgta ctggcacttc ttcatatacg
    30361 atggattgcc aatctttacg taaaaatgcg cgatagttga tcaagaattc ttctacatcc
    30421 tttactaatt cttctgcaaa gtcgtcaaat agttcttttt ttatctgtcg cttttttgac
    30481 tgcccattga tgtgttcact tattttcttg atttcatttt ccaatgcttg ataatccgct
    30541 atgtaaatcg gcatttctac ttggcgaaat tctgcgagcc gctgagctaa ttttatatcc
    30601 ggtaaatatg gctgtttcat ccgacgattt tggtacaaga caaaacagta tttttgccat
    30661 agtccttcag gaaaatcgat attcttctgc ttaagccata ataattcgtc taaggccaga
    30721 tcaacttttg tcattccctg atggatcgaa gctgccactg tcggagagac atgcaggtga
    30781 tcggcaaagt ggttgatgcc tagttttagg atctttccgg tttttggcga tttaacgatg
    30841 tattgcactt ttaattctct accacactca caatacaatt tttgctggct tcgttttgtt
    30901 cgaaaaaacg gattgatctt gaagtcagaa aacacccaat ctgttgcagc taaataatgt
    30961 tcttccataa aacgagagct gattagatat cggcggtgct tatcgactag ttcccgctgt
    31021 tttttcgtta attttgcata gatctcccaa cgttgactgc ttgtgtattg tttagaaatt
    31081 ttgatgggat agcttttgct gatttctttt ggaaaccatt cttcaaagac ggattgccat
    31141 tcttccacgg ctgtttcttt cctttctaat tacaaaaagc tggctactaa cataacgtta
    31201 gcaaccagcc agtgtttctt gctaaattag tgagttgttc cggcatttcc ggaaatatct
    31261 tctttatttt ctactgctgc ttcaactgca taagtcaaag aagaagcaat gtcttctaaa
    31321 gacatagaag gcgtacccac tggacgatcg atcacttgat cgggtgagaa tggcacgtgg
    31381 atgaacccac ctcgaattgc cgggaattgt gtttcgatca aatgaagcaa acgatacatg
    31441 atatcattgc agacaaatgt tcctgctgta taagagatgt acgctggtag accatgatct
    31501 tgtacatttt ttgtcattgc tttgattggc aatgaggtaa agtaagcggc tggaccgttt
    31561 tcttctaaag ccgtatcgaa tggctgattt ccttcattat cagggattct tgcttctgct
    31621 agattgattg cgacacgttc gaacgagact gctgaccgcc cacctgcttg tccgacacag
    31681 atcacgacat ctggttggtg ctgttggatg gcttctttga ctacttgact gcttttttcg
    31741 aatacggtcg gtacttccac tttgatgatt tcagcaccgc tgatttcatc tggcattttt
    31801 tttactgctt cataggctgg attcactttg tccccaccaa atggatcaaa acctgttact
    31861 agtactttca tctgttgttc ctccgtccga ttgatttaac atatggttat cttttatcat
    31921 accattttcc ttgaacagcg taaataaagg aaacaatagt ttaaataaca taacagatca
    31981 aacttaatat attcttataa aaaaatagtg ttccactaca atgttagaag aggttgcgac
    32041 aattattctg tcacaacccc tctcttctat tatttaaata gaatcatata agcaatttgg
    32101 aaaactagca taaataacgc aataaacaac tggtttttga tcactccgta tttgtctttc
    32161 atctccagca tcgctacagg cacgatattg aagttagcag ccatcggtgt cagcagggtc
    32221 ccacaaaaac cgcaagtcaa tgcaaccatt cctattaacg ctggattcgc tccatgacta
    32281 aatacgaatg gtgcaccgat cccaaccgtc atcacggtga tggccgcaaa tgcattcccc
    32341 atgattaccg taaatatcac catgccaaga gcgtaaatga tgatgccgat gttcacattt
    32401 ccttctggaa cgatcgtacc tactgcattg gaaatcactg tcccaactcc agcgcttgta
    32461 aataccgcac ctagagaagc taaaagcatt ggcaacatgc ttaacggccc tactgtgccc
    32521 agcatatctg ctgcatcatc taaaaatgta cttggtcgat tatctcgaga aaagaacatc
    32581 aagattagca tggctgcaaa aacaccgacg cccacgccga ctaaagcacc taaattggta
    32641 aatagagcaa acaagatcgc aaagacaccg atactcaaag ctggaataaa aattttcatt
    32701 cctagtttgt ctgacatttt ttccatatat tcttttgatg gaagccggct ttctcctttg
    32761 ctgactttct tagcaattgc tggtatggtc atcgcaaaga tcaagccacc gttcacgatt
    32821 gccggaagcc atcttcctcc tgcaataact acccctaaga ccgtccagaa aaaggctgac
    32881 ccataacgat gaggattggt tttatctgtt aaatttctta ctccagtgta aatcaatacg
    32941 agtcccatca cgatataaag gatctccagt aatttatcgc ctaataaaat atcaggattt
    33001 gtaaaaaagt ccatcttatt gcccctttcc tggataatat ttcttcgcta atttcttttc
    33061 tttaagatag aagtagacac ttgccacaac taaagccgtg atagccactg gaacttccac
    33121 taaagcaagc tgtcccaatg ttacgtcata tcctagatct ttcagtgttg attggactag
    33181 caacgcccct gctcctccga tgaatagtac ttggccaaag aaccagcaga cattttccat
    33241 agctgaagcc atacctttta gttcttcttc atgcttagga tggacttcta acccttttga
    33301 ttcgatggtc cccaatgcca tcggtaaaat gattggacga acaaatccag caactccacc
    33361 aaaactcacg ttaaaagccg caaagatcat tcgaaagatc ccataaatat ctacaatcat
    33421 accagcagat actttcttga atcgcttgat caaggaagcc gctgattctt tcagaccatt
    33481 tctttcaagc gttcctgttg ccaacataat gatgataaaa atcgccatcc cgcgattgtt
    33541 tacaaaactt gtacctaaaa gttctaacat tccttgaatg ccaagaccgc cagtcaaagc
    33601 tgtaacaact aaagcaacca ttacgatcaa tatcgagtct aatttaaatg caaacccgag
    33661 aataacaatc aagacgccta aaaggctaag ataattttcc atatattttc ctccataaaa
    33721 tgacaatatt tttttaatta taaagcaaaa cacataaaaa atatagttgt tttctcacga
    33781 aatagacact atttttaaat atttttcgtt atttttttat tatataattc accttactta
    33841 ttgtaagata aagctttttt taatttattg cctgctaata tataaaaata aggtctagtt
    33901 actgtttttt taaatgatta ataaataaca actcctttct atgaaattgc aaagcattca
    33961 tcttcttttc tgctaacgat atatccgtca atgcttgtta cactttacta aaaagtatga
    34021 gattgccgat atctggatag gtgtgctata ctacagaaaa agatatacga tctgccaccg
    34081 ggtagtaaaa aagaggatga tcattccatt aggtctttga agggatgatt ctcctctttt
    34141 tgtgtatttt ttatattgaa ggaggaacgt atgtatggca tggatggaat taatcatagc
    34201 aggaattttg gaagtattct ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat
    34261 caattattct ttttacacag tgattggaat gattgctagt ttttactttt tgtcaaaggc
    34321 aatcaaaagt ttgcctatga gtctctctta tcctatttgg acagggattg gcgcagttgg
    34381 ttcggtcatt attggtgtgg tcttgtttca tgataagctg aatctttcca cttggttctt
    34441 tgtcgggttg ctgctcatca gcatcattgg catcaaaatc actagcggtc actagatact
    34501 tagacaagca gacaaggact gatcagaaaa agcggggctt tttatttgat attcctcttt
    34561 ctatgaaaaa acaagaaaat aagactaatg acctagcgac ttctctactc atcaggtgta
    34621 tgcttgtact atctatagaa aaggagtgaa tatttttgaa aagtctttcg gtccaagaat
    34681 tatccaagaa ctttggtcac aaacaagccc tcagcgatgt cagttttagc ttatctcctg
    34741 gcaaaatcgt tggattagta ggtcctaatg gcgctggaaa aacaacgatc atgaaagcta
    34801 ttttaggact gatccattac tcagaaggaa cgatccgtat tggtgaaaat gagatttctc
    34861 cctctagtca taaaggatta gaacaagtag gagcattgat cgaatatccg ggtatctatc
    34921 cattcttaac aggttatgat catctgaagt tattctctga aacaaacgat gtatctgcta
    34981 tcgacactat cgtgaaacag ctcaagatgg aaaaatacat tcacaaaaaa gcgaaatcct
    35041 actcgctggg aatgaaacaa aaattgggaa ttgctcttgc cttactgaac catccgtcta
    35101 tcgttatttt agatgagccg atgaacggtt tggatccaca agcaacaaga gatgtgcgaa
    35161 aaatcattac cgacttggct actcagggca cctctttctt gatctccagt cacttactca
    35221 gcgagcttga aaaaatggtc gatgagctga tcctgatcaa tcaaggaaaa atcatcaaac
    35281 aatgtacaat ggctgaattg aaccaaacag cgcaagattt catcgttatc tctacgacaa
    35341 atgatcaagc tgctcgaacg atcttgacag acgctggata tcccttggaa gatcctgata
    35401 aaatcaagct tctaaaaaaa aacgaagagt tattaggaac tgttatccag ttattgtctg
    35461 cagaaaatgt ccatgtgaca gatgtccagc atctgacgaa tgatttggaa tcttccctat
    35521 tggaaattct ggattcagta aagggggaag aagaaaaatg acgttgctta taaaacaaga
    35581 attatttaaa ttgatcaaga aaaagtcaac tgctgtttta tctgttctat tagttgcttt
    35641 gctcatagga acagcacttc tagccaaaaa atacacgacg atcattgatc cagttgaaat
    35701 gactgctcag cttttcagtg ccacatcttg gattgtcttt atcatgattg ctgctgcaag
    35761 tacgatcatt tcaatggaat cacaatatgg tacattgaaa aatctccttt atcgaaagta
    35821 ttctcgagga gaaatattag taagtaaatg gatcacatta gttatttatt ctatttattt
    35881 atacctttta gctattattg tcaccgtgct gatgaaactg atccttttcc cttcgatttc
    35941 ctttacggag aagatttcta ctggtcagac actgatccaa agcttattca cttatacttt
    36001 gggtagttat atcggtttat ggctaatttt aagtttagtg ttgatgattg cttgttttat
    36061 caatagttcc ggtgcctcca tctcagcagg gatcgtcttt tattttgctt catcaatcat
    36121 atctggtatc ctcatcgcac tcattcaaaa atgggagtgg atcaaatgga atccaatcag
    36181 tatgctgaat ctgcaaaacc aaattggcaa cgaagagatt atgaagcaat tgactcatct
    36241 ttctactaat caaatgctgt ttggcaattt agcttacatt gctttgttct tagcattggg
    36301 atacctcgta ttcaagcgaa aaaatgtatg atggttcatt aacaaagaag ttgtgacaga
    36361 agtggccagt ttcaagaaat ttcggcttat ttttagaaga agctacttct tgaacaccat
    36421 ttattcaaaa aaagaagcga tgacacgaat gttgtcaccg cttctttttt gaataaacca
    36481 caggcttcac agcctctctt tatacttcta tacgtgaagc atcccagtgg caaataaaac
    36541 cattgcgatt actccgacca tcgtcacacc gataagtaaa atcttatcta agtggatatg
    36601 ttgtacaggt tcgttttgtt ctttacgtgc tttgaggtaa aaataaatac ctggtaagaa
    36661 aagaacaaga acaagcatca cttcattaaa ggctacaact agcatggccc atacttgaaa
    36721 agcacttgct aaaaacccta ctaaaaattg gacccactct ttccgctgat aagaaagctt
    36781 catttggtac agccctaaga aaagccacgt gaagaaaatc gctgctgtac caagtgtata
    36841 agcaaagtta tatgcactgt cagtgaataa taatgaaagt aaaaagacat tcgtacataa
    36901 cgtcgtcaat acgatagcat aagttggtgc cccatttttg ttgagttttc cccatgcttt
    36961 aggcaaaagt ccgtcctcag ccatattccc cattgtttca atcggcaaga ttgtccatga
    37021 gagccaaacc ccaactaaag aaatgatcaa accgccgttg atcacgaccg ccccccattt
    37081 accaaccatc atttcaaata catatcccat agcaggttgc gctttgatcg tttgcagctg
    37141 attcatcgtc attgtgccat atggaagcat ggaaaccaag acgtaaataa tgatcaaacc
    37201 gaatattcct agaatcgttg ctttcccaac atccgattgt ttttttgctc gtgaacctac
    37261 gacagaagct ccttcaatac caacgaatac ccagagcatc accatgatgc aactcaatac
    37321 ttgactagaa aacgggatcg gctgcccatt cgcattcaat atgatatcgc tagaaaagtt
    37381 attaatgaat tgttggatcg aaaaggtgct gatagctact acaataaata aaaataatgg
    37441 cactaattta caaaatgtga cgatagcatt gacaaagcta gcattttcaa ctccacgatt
    37501 cactagccat gcatatcccc ataagaagat cgtactcatc acgatcgatg ccacattttg
    37561 accaccttcg aatacgggga agaaataacc taatgcactc atcaacaatg ttgcgaacgc
    37621 cacgtttcct aaccagcatg tgagccagta accccaaccg ctgataaaac cagcgaaacg
    37681 gccaaaacct tcttctgcgt aactaaagat cccgcctttt aaatctggac gttttttatt
    37741 gatattattg atcgataagg agagcatcaa cattgctgtt cctacgagta cccagctgaa
    37801 gatagctggg ccaccagccg atccggagat gtccgtcata ataccaaata cgccacctcc
    37861 aatcgctgaa ccaatcacta aggcagcaag tggaatcaaa ccaattcctt ttttctgttg
    37921 ctctgtatcc atgtaagcat cctccaaagt aaaaccgctt ttatcaaaaa gtataacaaa
    37981 ccttttttat ttttacttaa ttaataaaaa ctttaataga aatataattc taccgacttt
    38041 tttctcatta tacaagcaaa gcgcctagtc cctttccaaa ttacaccacc aaaaaaactt
    38101 aaaaagatgc tatactaaag ggaaagaatt gggggatgat ccatgaacat tttatatata
    38161 tcgatttcag gaaatacgcg tgcttttgcc aaacatctag cagaatatgc tgaaaaaatg
    38221 catgcagaag atcctgtcaa tcctgaagtt actttaaaag aaatccatga aaatagtgat
    38281 tttacgaaag aaacagaacc attttttact ttcgttccta cttatttaga cggcgggaat
    38341 gggttagaca acggtgacac agaaatcttg accgaaacga tgcgcgaata tctggaatac
    38401 gaagacaatc ataaactttg ccttggtgta gtcggtagcg gtaataaaaa cttcaacaat
    38461 caatattgtc tgacagccaa acaatacgca caaagattcg gctttccttt cttggcagat
    38521 tatgaattac ggggaacacc atctgatgtg acacgcgttt atcaaatttt ggcagaaaaa
    38581 aataaataac acaaaagaag aagctgaaaa ataaatttcg gcttcttctt tttatgttct
    38641 ctattgtttc tattcttatt aagctttgac atccatcaca ggtgcttttg ctttcgcttt
    38701 acctgttttt tccaagacaa agttttttac acgatccata ttggttacca agacgatcat
    38761 ggaggtttct ttgtctgctg ctttgattgc ttctaagtca gcttctgcta caagcgtatc
    38821 aggtgtcact tgctgtcctt ctgttacttt cacatcgaat ggcgtaccgc ctaagtcaac
    38881 tgtgttgatg cccatgtgaa gcaagatttc taagccattt ggtgtcgtaa tgccgatagc
    38941 atgttttgtt gggaaaacag aacttattac tccagtaacc ggggaataga tatgtccgtt
    39001 ttctggttct acaaagtaac cttcacccat cattttttgt gcaaaaactg ggtcacttgc
    39061 tttatctaaa gaaacaactg ttccgtcagc aactgatgat aagatagtca cactttcagc
    39121 tgaagcttga ggtactgctt tttcaggtac ttcatttact gtggctgttt cggtcgctag
    39181 ttcttctttt tctaaacggt ctttaggaat accgaagaag aatgtagcaa cgaagccacc
    39241 agcgtaagca gccaataaac caaggatata gcctaaccat ttaccatcag agatcaatgg
    39301 aatcaaagcg gctcctgaag gtccaatagc gattgctccg acatttccga tcattccgat
    39361 cactgcccca ccgatacctc caccgataca agcagtaata aatggacgtc ctagaggtaa
    39421 tgttactccg taaatcaatg gttcgccgat tcctaagatt ccgactggta aagccccttt
    39481 gatcatttct gttaattctt tgtctttttt gcagcggatc cataatgcca atgctgcacc
    39541 cacttgtctt gctccggcca tcgcaaggat tggaagcaat aaggtacgac cagtttcatc
    39601 aatcatttgt acatggattg gcgtcaagat ctgatgtagt ccgaacatga ccattggcaa
    39661 gaataatgta cctagaacga agccggcaaa tgctcctcca acaccaagta cccagttgat
    39721 cccaccgacc atgccgtcag aaatgaaccc tgctaaaggc atgatgaaga aaatttcgat
    39781 cagaccgatg gcaatcaggg aaagagttgg cgtaacgatg atatctaccg catcaggtac
    39841 tactttatgc agttttttct caacgattga caataaccaa actgcgaaga taacacctaa
    39901 gatcccgcct tgtccagttg caagagcttc accggtaaac aagtttttca gtggtgcttc
    39961 tggattcatc cctgtcagca tcacaacagc cccgataact ccaccaagcg ttggcgttgc
    40021 accaaaaact tgtgcagcat tgatacctgt atagataact agatagctaa acatcccgtt
    40081 tttcaagatg ttcatcacat cgatatattg ttgccatgtt cctgcgtcta gtgttccagc
    40141 tgtgactaag tttgctaaaa ctgcggcaat ccctgccacg atcccagtgc cgacaaatgc
    40201 agggatcatt ggtacaaaga tattagagat gtcttttaat accgatttga ttttagaggg
    40261 tttttgtttc gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta cacggtcttt
    40321 tccagatgca gaagaacttg ctgtagttgc actagcagaa ggaagttttt ctcctaattt
    40381 cacgcctgct aagtcaacca tttcctgcgc tactttgttc actttcccag gtccgatgat
    40441 gacttggagc tgttcatcct ctactacgcc aagtactcct ggaatcgctt ttaattccga
    40501 gacatttaca agatcatcat tgatgacctt catacgtaca cgagtcatgc agtggacaat
    40561 gctttcaaca ttgcccatac cgcctgctcc ttgataaatt tgttttgcca gttcttccaa
    40621 agtcaattcg ttttttgcca tggtcttttc cccctcataa ggttcctttt acaaaaccgt
    40681 ttgcttcagc taattttgct tgagctgcct ctttatcact atttgtcaaa atcatgacga
    40741 tagccagttt gacattttga tctgcttcct caaagtactt ttcagctgtt tcgtagtcag
    40801 cttctgtcgc ttgcatgatg attcgttttg agcgttcaat tagtttttca tttgttggtt
    40861 tgacgtcgac catcaagtta ttgaatactt taccgatacc gatcatactg atagtagaaa
    40921 tcatattcaa aatcagtttt tgtgctgttc ctgacttcaa tcgagtagag cctgtcaaaa
    40981 attctggtcc tgcatcgact tcgattggga aatccgcatg ctcgctgatt tctgcttgtt
    41041 tattgcaaga aatcgttcct gtaacagcac caatcgattt tgcgtaatct aatccaccaa
    41101 tcacatatgg tgtacgaccg ctagctgcga taccaataac tatatcattt tttgttaaat
    41161 tcagcttgat tagatcttct tgccctaatt cataagaatc ttcggctcct tcgacggcta
    41221 cagtcattgc ttcttctcca ccagcaatta ggcctatgac catttccggt tctacaccga
    41281 atgtcggtac acattcagca gcatctaata caccaagtct accactcgtt ccagctccca
    41341 tataaatcaa gcgaccacct tttttaaagg attcaatcgt gttggcgatc actggctcga
    41401 tacttggcag ttctttttca acagcagcgg ccactttctg atcttctttg ttcatgagtt
    41461 ggactgcttg agaaatactc atttcatcta atccaaaagt ttcttggttt ctgcgttccg
    41521 ttgttaggtt ctctaaattg attttgctca tttttatctt ccttcttttc ttataaattg
    41581 atagttcata ccagctttga tttggttgat cagcggtagt tcttctttga tgactttggc
    41641 tactacattc actttttcat ccgctggcaa atcatattta gttaattgga tctcacccat
    41701 atagcgcaaa tacttttcgt tatctaaagt aatagatcct ttcatacgct tagctgtatt
    41761 tcttactggg attggcggaa tctctcgaaa acgcgcatct gcgcttcgta tgacatctct
    41821 tgcatcatct tgccgattcg tatgttttcc taatatatat tcgtaaaaaa cttcatctac
    41881 aggttccgta tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt gctcctgaac
    41941 gtgttctgac agacctgcat ctccgatata aacaatatcc gtgttacttg ctaatagatc
    42001 aagagcacct gccagaggat ggatccctcg atgttcttcc aaagttggta atccttgata
    42061 cagtggacct ctcaactctg tatctcctgg tacaaagcct tgtacaagga atccttgagt
    42121 tttgagccat tgattttttt cctgatacca ttctttgtcc aaccctgttt caagacgtgg
    42181 ataataatta tgccaagctt caatctgtga gaaatcagcc gctgcttctt tcaactcatc
    42241 gatatcatta ggcgtgatcg tgctggcatt caaactgatt ttcaattgat gtgaccagtc
    42301 ggcgatctgc tgattagaaa tatgataatc cattcttaac cctgttacac caattgcttt
    42361 caagggtgtt agatcttcta atgaaaagcc agctcgtttc aatgcctctc ctgaaatatc
    42421 gaccatcaac tctagtttat tctttttcgc ttcgcttcct agttcgatca atcgattttg
    42481 ataagccgaa acatcatctt ccggtatatg catggaagtg aaaataccga caaagccatt
    42541 ggatgccatt tggcggatat actgtttctt ctcttctgac aaagaatcat tcataaatat
    42601 tgaaaagcca aacatattat ccctcttttt ctgtatccgt cttcatttct atgactagag
    42661 tatatatcta aagaaacaaa atttcaatac ttttgttaaa ataattaaat tttatttcta
    42721 aaaattggat ttataatgcc aaaagaattg acacagccac aaaaaaggca ctgctaagca
    42781 aaagcagtgc cttgaaacat tttatttaaa taacacaaaa gtctaattac tcataagaaa
    42841 ttaattctgt attgtctacc atgaatgtaa agaacccttc attaggcatt ggatgaaaat
    42901 aagcattgat cactcttatc aaggcattat gacagactaa caagtagaca ttttcttcgt
    42961 cttccatgca ttctttcaat aaaggaacga tccgtgcgta cacatctaag accgactctc
    43021 catttgggaa acgtacagca aacgctaaac gcgctttttg aaagttttca tctttgcttg
    43081 gcagtccgtc ataatcccca aaatccattt ctaccaatcg ctcatcagcg gtcatcggca
    43141 aattgagacg atcaactact gcttgtgctg tatctcttgc tcgttgaaga ggggaatgga
    43201 tgattttcgt gattggaaca ggcaaatcta ctaccttttc tgctagtgcc tgtgcctgca
    43261 tctgtccttt ttcagtcagc ggcacatctg ctcgcccgca aactaatcct tgtgcgttcc
    43321 aagttgtttc tccgtgtctc gttacataaa gcatgtgatt ctctccttcg ttgacaaagt
    43381 tctccttaac cataccttgt tgtctttgag attacaaggt ttagattttc tcaagagaaa
    43441 taatctcagt agatacttta tctagaaagg tttgatcatg ttccactagt aacatagcag
    43501 gtttcacgtt caaaatcagt tgttctaatt gttcttgatt gaagacatcc aaataattca
    43561 atggttcatc ccatatatag agctcagctg gttgggataa agatttagcc aattccactt
    43621 ttttccgttg tcccatactc atctgttcga ttttattatg aaaaacatct ctttccatcc
    43681 caagctttcg gagattattc aaaaatgctt gatagtctac tcggtttttc ttcgcgaatt
    43741 ccgccaatgt ccctcgattg tcttcataat tttggcgtac gtaactaatg gtcaaatgtt
    43801 tgggctggga tttttctcct acaaccttgc cgttgaatgt ccccagaaga taatggataa
    43861 tggatgactt tcccgcgccg ttaggacctg aaattgccat ccgctgatga ggttcgatcg
    43921 taaaatgaac tggctcgaat aacagatttt cataccctaa ttgaagattt tctacgctta
    43981 acagccgttt atgatgagac acttggctat tcatcgtcaa agcatcgata tactcgatat
    44041 cttttaataa tttttctttc tcactgatct gggtttccat ccgattcacg attgctttag
    44101 atcgtttcat cgtccgtgca gcatcagcgc ctactgctcc tttattcact cgcctagatt
    44161 ccgtatcgat gaatccgact tttttctttg ttttgtcccc ttctcgggaa cgagaccatt
    44221 ctgctttttc agctgctgtt ttctttagcc tgctgacttc ttttttcaat ttttcatttt
    44281 tagctatttc aaattcatca cgaagttttt tctgttcttc atagatagag aaattccctt
    44341 gataaagttc cagttggctc ttttcgattg ctaaaacatg gtccactact tcatcgataa
    44401 atccccgatc atgactcacc acgatgaatc cttgtttttt ctttttcaag taagccgcta
    44461 cttgttttct gccagagata tccaaatgat tcgttggttc atcgattaac gggaaatgag
    44521 tgtcatccac aaataagagt gccagtagga cttttgtctt ctctcctcct gatagtgtgc
    44581 tgaattctct ccataagatt tcaggatctg tttgcatcag ttggagctct ctttcaattt
    44641 cccatatctc aaaatccata atctcattta acacataata ggtcaaacgc tctttatctt
    44701 ttgtttgctg cgggaaatag acaaacgctt gctgatggat cacttgccct tgataaggga
    44761 gtttgttttg caggatattc agtaaagttg tctttcctcg accgtttcgt ccgataagtc
    44821 ctagtttcca ttgtgtgtca aagtttagat tggcttgttc aaataataat gtgccttggc
    44881 tgtcatagcc gaatgtcaga ttttttattt cgattttaga cattattcca tcacctcgta
    44941 aaaaaagacc agtcctattt tcagaactgg ctaaagagga tgcaagggtt ggggcacaca
    45001 aaaagaccca tacatcgaaa gaaccaagcc aatcccgaaa atctgcacac tgctcccttg
    45061 tctccacaag gtacagtctg aatgcagctt tcatcaatcg acttaatttt tcaatgcacg
    45121 gatccctcac tttgtcctat atttgaaatt acgatagcat aaagaaattg tttttgtcaa
    45181 ctacttcggg agaatgttga aaaatctgct gagtgtctgt cctaagattt ctgttttttc
    45241 taatgtttgc ctgtgttcca ttgcttcctc taaatcgatt ggtcctaatt gaatgcttaa
    45301 gacaatgggg agaaaatcaa agagtccttc cagctctcgg tctctacttc cgcaaatagc
    45361 gattattggt tttcgatatt tttttgcaag cactgctacg ccatatggaa cttttccgtg
    45421 gattgtttgc tgatcaatcc gcccttctcc tgtaatgatg acatccgctt gttcgatctg
    45481 tttttctaat ccagtgattt ggctgaccaa ctcaaaacca gagaccatct tcgctcctaa
    45541 caaaaaaagg gctccgccaa taccgcctgc tgctccgctt cctgggatgt catctaaatc
    45601 cacctcccaa acatctttta tctgctgagc aatcttttca gcttgtttat ccaagaaagc
    45661 taacgtcgta tccgttcctc ctttttgtct tccaaattgc caagcagctc cttgttgacc
    45721 tgtatatggg ttggtcacgt ctgcagcgat gatcactttt actttagaaa caagaacgtt
    45781 ccttcgatcc aaatgaagtt ttctagtaga taacaaagga tttccttctg tgtacccttc
    45841 taaagaagca cccaaagatt ggagtaatcc tagaccacca tcgctacatc cgcttcctcc
    45901 taaagtgaca atgatttgtt ccacttcttg aagcagtgta tctttgataa gctctcccaa
    45961 ggcataacta gaagcaagtc ggatggtttg atccgttggt ttatcaagta aatctaatcc
    46021 taatacttgt gcagattcaa tgactgcggt attttttcct tgccagttcg tcaataaata
    46081 actgattttt cgctgacgaa ataagagatc catcccatca atcgtacgcc aggttccccc
    46141 gagcgcgtaa tagattgctt ctgcagtatt ttctccgcca tctgcaatcg gcacattgat
    46201 ttttcttcct ttacggtctt cccaaccatt tagaactgcc tgatttaatt caaatgaaga
    46261 agcacttcct ttaaatgaat cgattgccgc caaaatatcc atctatatcc ctcactttct
    46321 tcttaagtaa gacacttttt ttcacttttt tcaaacaaaa caatgctatt ttcttctgtt
    46381 tttctatata ctaaaaggga actcattgaa taaaggagag aaaaaacatg aaaatcgcaa
    46441 tagctggagc cggagcaatg ggcagccgaa tcggattaat gttgcatcaa agcggaaatg
    46501 aggtcgtctt gatcgatcgc tggcctgctc acattgaagc cattcgtaca aatggattga
    46561 ttgctgactt taatggcaaa gaagtagttg caaaactgcc aatctattca ccagaagaaa
    46621 tcatcgaaag caacaaacac gtggatttga tcgtagcctt gacaaaagcg aatcaattag
    46681 atgatatgtt tcgttcgatc caatccatta ttactgagaa tacttatgtt ttgtgtttac
    46741 tgaatggatt aggtcatgaa gatgtactgg aaaaatatgt accaaagaaa aatatcttat
    46801 ttgggatcac gatgtggaca gctggtctag ctggtcccgg aaaagtcaca ttattaggtg
    46861 atggagaaat cgaactagag aacttggaac cagaaggcga agcatttaca aaaaaagttg
    46921 tagaagtctt tcaagaagca aaattgaatc caatctatag tcataatgtt cgttattcca
    46981 tttggcgaaa agcttgtgta aacggaacat taaatggatt atgcacgata ttggattgta
    47041 atattgctga attaggtgcg caaaaagcgg cagagggcat ggttcgaacg attgtatctg
    47101 agtttgcttc tatcgcagca aaagaaggaa tcacattaga tcaagaagaa gtgtaccaac
    47161 atattgcttc tacttatgat cctgacaaca tcgggttgca ttacccatct atgtaccaag
    47221 acctgatcaa aaaccatcgt ttgactgaaa tcgactacat caatggtgcc gtttggcgaa
    47281 aaggacaaaa atatgatatt gctacaccat attgtgcctt tttgacccaa ttagtccatg
    47341 caaaagaagg aatccttggc gcagaataaa aggctctctg atgagaatca gctggatcag
    47401 cagctggttc tcattttttt acccccatga aagttcaaaa agaaaaacac aattctcatg
    47461 ttgccataag aattgcgctt gtttattttc atctatgatt ggttggcact tttatttatt
    47521 tttaacagag gaaagatcgt attcatattg ttcataaata gcggagtcag tagtgaaaaa
    47581 acgttttgcc caattttctg catcaaaaaa ttccaacagt tctgatggga ttggttgaaa
    47641 tggcgtatgc aaaaattccg gtaattcatc taaatcacgt tcttgaagca aaaaaatatt
    47701 ttcagaatga taaaactgat aatgagctat ggcttgatta tctgtcacta gtttcttttg
    47761 aaaatacata gattcaaacg ttcgcaaagt cactccttgc tgcccttccc gcaacagttc
    47821 tagaatcccc ctcgattccc cggttttttt cagatattct tcataagtaa ggtatccagg
    47881 ttgttcattt cctcgaagcg gaagaataaa gtaattggtc gtaacacctc tttttgtaaa
    47941 ttcctgctta tatttttctg cccgttcttt tctgccgtta tttgttgcac caaaaaatag
    48001 atccgtcttt ggttcgctag aagaaaaatc catatgtttc gaataaaagg aactattatg
    48061 ttttagtcca agttctttcg cttctaaaaa atcaaaatga taaatctcat cgattgccgg
    48121 ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct ttaaagtggt tccaaaaaaa
    48181 taacacgatt tttgcattgg taaatggacg cacgcgtcga gccatctcca tatcaaatcc
    48241 ataatctaaa aaaacgatca gctgataatt tgccaactgt tgtcgagctg atttaatgaa
    48301 ttccgactgt agtaaatctt ttccggacca aacatctaaa gagcggatgg aaggaaaaaa
    48361 ataaagtgat ttatttttcg caattagcaa tacgtcgttt ttgttcatct gttccgcctc
    48421 actttctctg tcactttttc ctatgatagc ctgtttaaat agtaaaaagc cagtaaaacg
    48481 cttcctatta tttttataaa aatttaacat ttctatgaaa atacgatgaa ataaatcatt
    48541 cttccattaa aatatgccca aaagttttct gaatagttgt aattatctga aaatttgtta
    48601 tcattagtca ttattattat tactatttat tcttactatt tattcttact atgcattttg
    48661 attgtgaatt taaaagaaag aggttattta tggaagataa aaaattgaaa aaacaactta
    48721 gctcccgaca tatcaccatg ctagctttag gtggagcgat tggtgcaggt ctatttaaag
    48781 gaagtggcga agcgattgga atcgccggcc cttctgtatt gatcgctttt ttgattggcg
    48841 gcataatttt atatatcgtg atgaaaggtc tgggaaaaat tgttttaagt ggtggtgata
    48901 cgcaccacgg attatctgga ttgattcgtc catatctagg tgctcatgct gccgatttta
    48961 ccgattgggt ctactggtcc atgtggatta tcaatatcat tgctgaagct gttgcagctg
    49021 cttccttttt acagttgtgg ttcccccaca tcccagcttg ggtatttgtt tttatgttgg
    49081 ctgttttaac tactatcatc aatctttatt cggtacgatt attcgcagag acagaatatt
    49141 ggctggcctt tgctaaaatc agtgtcatta tcctattgat catttttgct gcttatcttg
    49201 tcggtcagca aatgttgggt actggtgttt tccctaccct tcaacaatta actgatcatg
    49261 gtggttttac tccccacggt atgaagggaa tcatcagttc tttgcttgta gttatctatt
    49321 cttacggcgg ttctgagttg attgctatca cagtaagcga agcagatgat cctaaacgtg
    49381 cgattcctaa agcaatcaaa ggcgtcatgg gaagaatcat ttccttttac attattcctt
    49441 tatttttgct tttgattatt tttccttgga atactttagc aggcacgaac gttagtcctt
    49501 ttgtgatggt atttgaaaaa atgaatattc catttgcggc agatatcgtc aatttcgtga
    49561 ttgttcttgc tctgttctcg tctatcaatt caggtgtata tgcttcttcg agaattttgt
    49621 tcttccgctt gaaagatcgg aaaggttcct ctagaaaact agctgtttta aacaagcatc
    49681 aagtaccgca acgtgctgtg ctgttttgta ccagtgttct ttatctaggt gtggctcttt
    49741 cttattttgt cggtgacaaa ttatttggct atctggcagg ttctctttcc tatacagtat
    49801 tattgatctg gatcattatc agtgctgcgt cctttgtact gacactaagg aaaggaacgt
    49861 tatgggacaa aggaatcagc ttttttgcat tagcagtctt atggttgatt ttccttggta
    49921 tcctattcac gaactccatt ggggtaactg tattgaccgg tttgctttac ctgttcattt
    49981 atttcagtta tcaaaaaaag aatgatgctt tcgtcttgac gaatgaatga aaccaataga
    50041 gaattttatt ctatttatat tgaatatctg gagctattga aaaaaaggag ctgcgacaaa
    50101 aattgttgca gttctttttt ccgaataaac ggtgttcaaa aaccatctgt gcagattaat
    50161 tgattgttct ccacgctcat gacatgatag aagaaaggaa tgaagaaaaa ggagtttgtt
    50221 ttaaatgaaa aagtattgga agtttttgtt gatgattggt gtttcgacgg tagtcatgtt
    50281 tggtatgatg tatttgaatg tttatgcgct tgatcatatc ttcttcagtc gcacacgtgt
    50341 attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc atgttgttgt tcatgtggaa
    50401 aatgtatgaa aacaaaaaga taaatgctat gattttagcc gctagtcttc ttgtttttgc
    50461 cggttcctta tttatggtac gaagccaaac aactgtcaac gatacggcgt ggatgaaggc
    50521 gatgatccct catcactcta ttgctatctt gacaagtaaa cgagctgatt taaaagatcc
    50581 tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa gaagaagaaa tcgacaaaat
    50641 gaaaaaaatg atcaaagaaa tggaatgacc attatccatc caaataaaaa aagaggatgt
    50701 tccagaagtg atcaacttca agaaataaga agaagctact tctggaacac cgtgaatcag
    50761 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
    50821 catatttttc gtttggttag gcttattacc gcaaatgcaa gcttttgaac accgtttatt
    50881 cgtaaaaggg agtgtgacaa aagtcttgtc acactccctt tttacgtttt tattgttcta
    50941 attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttctttgag atcccccagc
    51001 actgaccatc gaaaacacag gtgaaagcaa actgaaaaac acgaatggca agtaagtgag
    51061 tgtaggaatg ccaaatgtac ttgctacgaa acttccggcg attccccaag gcactaagta
    51121 gttgattact gttccaccat cttctagtgt tctccctaat acagttggat caaatcctgc
    51181 tttttcgtaa gtttctttaa atgcattacc aggcaggata acagatagaa attgttctcc
    51241 aataaagata tttactccga tgcttgtcat caatgttgta aaaatcaatt ttggggtgtt
    51301 cgtcatcttt cttgatactc gttccataac ggttgagatc aacccaaatt cgatcaacaa
    51361 accacctaat gacaatgtta gaataatcaa tgacacagtt ggcatcatgc tttcgattcc
    51421 tccacggcta agtagtgaat cgatttgttg gtttccagta tttgaaacaa atcccgattc
    51481 gatgacccct gccaacttcg ttaaggaagt ttgtgggttt tgaataaaga tcattcctac
    51541 tgctacagca atattcaaca aaatcgtcaa aattgccggt acctttttcc atgcacacaa
    51601 gagcattaaa aatagaggaa tcagtgacca gatcgaaata gtaaagtttg cttctaaaat
    51661 atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat
    51721 gaataaaaag aaagaaacaa caaatgccgg aacagttgac cacatcatat gcttgatgtg
    51781 tttgaacaaa tcggcatcaa caatagctgc tgctaaattt gttgattcgg ataatggaga
    51841 catcttgtct ccaaaaacgg cacctgaaac aatggctcca acaacaaggg ctggatttag
    51901 tcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc
    51961 actccctaca gcgctaccta cgattgcaca aacaacaaat acagaaggga cgaaccactg
    52021 gacactaatc aatttaaatc caatgaccat gagggttgga ataattcctg cttgtatcca
    52081 tacagcaatc aatgaaccga ctaaaataaa aataaagatt ggaatgatcc ccggtttgat
    52141 gccattgatg atcccttcgt gcagccaatc tgtcgggtat ttttttatca ctgcgtacaa
    52201 catgaccatt ccgatagctg ccaaaatcgt gatattaggt gaaaccttta atccgacaac
    52261 gccaatcgca atgcctaaaa caatgaccat caatacaagt gttgcttcta aaaaacttgg
    52321 tgttcctttt ttttccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag
    52381 ttaagatatc ggattactcc gccacaattc atccgcgatt cctcctttat aaaaataaaa
    52441 agacctgctt tcaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc
    52501 tgtttttcac tttcaaacga ttattcaaat gtatggactt tccatgtaat tcgtcaatta
    52561 ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac cactactgcg
    52621 tgaaaagttg acttgtatga agtttacctt ccataagatc atttgtcaat gaaaaagaaa
    52681 atattttttc atttttctga aaattatttt tataactttc gcgcacaact ttatcttttt
    52741 actagaaagt agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgtttga
    52801 ttttttctaa tagcttgggt tctctcaata aatctagagc tgtattggca agtaattccg
    52861 ctgcaatcgc aatagaagca agtccttttt cacttttagc agcagctttg aattcttctg
    52921 tatgtccggc aatatatgtc tcagagattg ctacagtagg ctgtatcgtt ggaataactt
    52981 ggctaacatt tcctacatca gaagaaccta gacttgttct ttgttccgtt tctatttctt
    53041 catcaggaac ccctgcttct tttgcatgtt ggaagaataa gtcatcgaaa gaaggcgtta
    53101 cgatgacatc atcgactgca ttttggaaca atccaaactc aaaagtcgta cccgtgctta
    53161 atgcagcacc tcgtacgata ttttccactt tttcatatac tttgtctaat gtttgtcgat
    53221 ttcctgcacg taaatagaaa cgtccagcag cgtagtctgg tatgacattg gccgccacgc
    53281 ctccatctgt aatgattcca tggatattga catcttttgg caattgcaag cgtagagcgt
    53341 tgattccgtt aaacacttgg atcaaggctt ccaaagcatt tactcctttt tcaggtgctg
    53401 ctgctgcatg agaagcgaca ccgtaaaatt tgatatcgac tggatcattt gctaaagatt
    53461 ctgtcgtttt cccataacga aaagctggat ggacacataa tgctgcatcg acatcctcaa
    53521 aaaaaccttc ccgaacgaag cttcccttag ctgatccatt ttcgcctcct tcttcccccg
    53581 gagtaccata cacacgaatt tctccgccaa tctcatcaat gaattgtttc actatgctgg
    53641 ctgctaatac ggagtaagta ccaaataaat tatgtccgca tgcatgacca atacctggta
    53701 aagcatcaaa ttctgccaaa aaggcaattg tcggacctgg tttattggaa atataacgtg
    53761 catcaaagcc tgttcggtgc ccagctactt ctttttctac ccagaaccct tctttttcca
    53821 attgtttgat taaagctgct gaggaataaa attcataatt acttacttca ggatggtcgt
    53881 ggatagctag tgcaagtgct tgatattctg gtaaacggta ttgaatcaga tccttcaatt
    53941 gatttgttga gagtgtggtt gtcataaaaa tttcctccta tgctagtcgt tttttcttat
    54001 tttctcccga atgtttccca cgcaggtacg cttgaacctt ttgatgtttt ttcgattact
    54061 ttggctgttt cttctgtttg ataggcatct actatttttt gatacgtttt attgtcttca
    54121 tcttcttttc ttgcaacgat gatattgaca taaggacgtg catgctcatc gactggttct
    54181 aagaagattg catcagaagc gggattaaat ccagcatcta cagctacacc gctattgatg
    54241 actgaaatat ctacatctga caaagcacgt gctgtttggg aagcatctaa ctcagaaatg
    54301 tctagatttt ttttattctc ggtaatatca gaaacggtag gcgcttggcc ttttgccttg
    54361 tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc attcgtgaca
    54421 tcattaggga ttgcaacttt tgctccatct ttgatatcag ccacatcttt taacgtattg
    54481 gaataaattc ccaacggtgc attaaccgta ttcccaatcg atactaaatc agttccgtgt
    54541 tcttcattgt aattgtctaa aaagaattga tgttggaagg aattcaaatc gatcgatcca
    54601 tctgctaaag ccgcatttgg ctgattgtaa tctgtaaatt tcacatattc caaatggatg
    54661 ttttcctttt tcaaacggtc tttcacatcg tcccatacat ctgtatcttc cccaatgatc
    54721 cctagtttta cggtcgtttc ttttttttca gttgtagctg cttttccttg gctgcatcct
    54781 gccaaccaaa taattgctac aaatgtaatg actgcaaaac gaataatttt tttcattttt
    54841 tctcctccat tttcaatgtg tgactttttt aatcagttga ttactgataa attgactcaa
    54901 aaaaactaag accagaatca ataatgtagc gacaatcgtt acatctgttt ggaatcgatt
    54961 atatcctctc gatatagcaa gattccctaa tcctccggca cctactgctc cagccatggt
    55021 agtcagtccg atcacattga tgatcgtcaa cgctgagacc cgaatgatac caggtaaccc
    55081 ttctaacaaa taaacacgta aaatgatgcc gattgggctc gtgcccatcg cttctgctgc
    55141 ttcgatcacc ccaggatcaa cttctagcag tgcattctcg atttgacggg caaaaaacgg
    55201 gacaacgcca aaaatcaaag ggactaaagc tgctttggca ccaatcgtcg ttccaactaa
    55261 gaaacgagta gtcaaagcta aaagagaaag caagataatg aaagggatgg aacgaatgat
    55321 attgatcaac ttatcaagta tttcgaacag cagcctgtta gctagtaatc catttggacg
    55381 tgtcactact aacgtcacac ctaagatgat tccgatgatc ccaccaatga atgccgtcca
    55441 aaaagacata tatagtgttt gctttgtcgc ttcgatgaat tcatccggaa tcaaatagac
    55501 attcggcaaa tatgttttaa aaaattcagt cattttagtt tcctccttcg atgactttga
    55561 tacggttatc taattcattt ttttgtaaaa tagttacttt tacaccagat ttaatcaaat
    55621 aatccaatgc tttttgccgc tgtttggttt ctccagctaa agtaacgatc aaacttccta
    55681 agggtgtttc ttgtaatagc tctacattac cgtataagat attcgctgtt acttggtact
    55741 tgctgtaaag atgagcgatt agaggttcgt tcgtctggtt gccgatataa cttaattcca
    55801 ccaaccattt atttgcaatc tgatcggcaa aagcagcatg ggcactgatt gtttccaatg
    55861 cttggtcaag gtgtgtcgct gtccgaataa aatctttagt caattcttct tcaggatgac
    55921 tgaaaatctg gatgctgcta cctttttcaa tgattttccc cgcttccata accgctacct
    55981 tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt gttagaccta
    56041 gctgacgatt tagttttttt aacagtgcaa ggatctgaag ggtcgttttc ggatcaagtg
    56101 cactagttgc ttcatcgcat aacaacactt taggttcatt tgccagagct cgtgcgattg
    56161 ccactcgttg tttttgcccc cccgacagtt gtttaggata cgcagatgct ttttcttcca
    56221 gaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttt gattttccag
    56281 aatactttag agaaaaatct acgttgtcaa aaatcgttcg actttccatt agattgaaat
    56341 gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttcttttgga gagagctggc
    56401 ttaaattcgt ctgattgacg atgacttctc ctgctgttgg tttctgcaac aaattcatca
    56461 cgcggactaa cgtactcttt cctgcacctg agtaaccgac gatcccataa acatcccctt
    56521 tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac
    56581 atacagaaat atctcgcaat tcaatcatta acattcctcc ttgtctataa aaaaagcatc
    56641 ctcaagaaaa tgaaaatagt cattttcttg aggacgcctt tttgcgtgtt accacctcat
    56701 gttcaagctg tgttcacaca caactcctct tcaagtacca tcatactcta gcaagatatc
    56761 ggtcgcagcc gtcaccgcct tacatctatg ctcgggatga attgctcgat ggccattttc
    56821 caatagtcgt tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc
    56881 gtacttacca tttcaatgca aataatattc aaaaaaaaaa tccttttact aacataaact
    56941 atgtcagtaa aaggacgacg tatcgtggta ccaccttttt tcggaagttt ccttccctca
    57001 agcccaacat ctcaaaaaga gagattggcg ttcgttttaa cggtcgaaca tttccgaatt
    57061 tgcctactta tcgacaaatt attctccaag gccattttca gcttttctta cttgactctt
    57121 cacaccagcc agagcctctc tgcaaagttt ccaagcttac tttccttctc aacgaaatca
    57181 atattttgtt gttgggaata atctacagga ggaaaactga aaagtcaagc atgaaatcca
    57241 aattattttt tctatattta tttttctgtt tttttatcta acatttcaat aacagttcga
    57301 agatcttttc cttccgcaag aaggttcaat ttttccttct cttcgacgct ataatttcga
    57361 acaaaactgg aatcgggaat caaaacaacg cctagtcctg cacgtgaagc ggctgtcgct
    57421 cctactaaag aatcttccac tgcaaaagct gaaagagctt ctaatcctgt tcgttccaaa
    57481 gctgtcagat aagggactgg tgaaggcttg ttctccgata cttcatctcc gaacgtaaac
    57541 gtatcaaagt aatcggctaa ttgaaagtga tccaagatat ctgttgctct tttcgtaaca
    57601 gttgtcgtag caatccctag tttcatatga cgttctttta agtctgtcaa tatttctttt
    57661 gcatagggtt tcaattggat agctccactt gtcagctgtt gataaaaata ttcttcacgc
    57721 tttgctcgga cttcctgcac tttttcagga ctgcctgctg cctgcactaa aatatcaaaa
    57781 ctttgtttcc agttttttcc gccccaagcg tcaacgattt ctttcgggat atgaatatca
    57841 aattgctgga atccccaaag ccaaccatcc cgatacgttc tttccgtatc cactaacaaa
    57901 ccatctaaat caaatattgc acctttcatc tatacattaa tcctttccaa aacaatacac
    57961 ctagtttagc atgactgatc caaaatgaac agaagcatag agcccagccg catcacttat
    58021 aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag cgccagataa
    58081 gcgacgactg ttataaacaa ccataaccag taattgccag ggagcggcat caagcctaac
    58141 tcagcaccaa atcctgtgaa tggaaggact gaaccgacaa tgattcctaa tgtagtgata
    58201 accagcatgg caaatgttgc attgctttgg ataaatggta ttttcggtgt acgcaatgca
    58261 tggagtacta atgtttgtgt ccacagggat tcgacaaacc atcctgcatg gaataatgcg
    58321 ataaaaatca cttgttgtga gctatccaat gtatggaaac ttccaccaac tacagcagga
    58381 caaataacaa aatacatcaa caagtaagta gtgatatcaa aaatcgaact tgttggacca
    58441 aaataaacca tgaattttcc aatactagat gcattccatt tctttggctc atccaaatat
    58501 tctttatcca tccgatccca tggtaaggaa atgcaagaaa cgtcatagat cagattcaaa
    58561 aatagaagtt gcaaaggcaa cattggtaaa aatggtaaga acgtactggc aatcagtacg
    58621 gaaaacatat tgccaaaatt agaacttgct gttgccttga tatatttcat gatgtttcca
    58681 aaagtttctc ttccagaaag aaggcctctt tccaaaatcg tcaaatcttt ttctaaaagg
    58741 atcacgtctg ctgattcttt tgcgatatcc acagctgtat ccacggaaat accgatatca
    58801 gccactttca ttgctggagc atcattgata ccatctccta aaaatccgac agtatgccca
    58861 tttttacgta aagctgtcgt caatcgtgct ttttgctgag gattcaattt tacgaatact
    58921 tcgtgttctt cagctacttg cgctaattca tgatcattca tttccgagat tttctcacca
    58981 gtaatcaatt cattgatttc taggcctact tgacggcaca cagaccgtgt gactaattca
    59041 ttgtccccag tcagaacttt tacagctaca ccatgttctt ttaaagcttt caacgcttgt
    59101 tttgttgttt cttttggtgg atcaaggaaa gcaagataac caatcagaac catgtctgac
    59161 tcatctttga ctgaaaattc tccaacaacg cttggattgg tcttttgtgc gacaccaatc
    59221 actcgtaagc cgtcttcatt aagtgattgt acttttgcaa gcaccgcttc ttttcgttct
    59281 tttgttaatg gtgcgacagt accaccaaga tcaataaatt tagaaatgtt caacatttct
    59341 tcaatcgcgc cttttgtgat catctgtgtt ttgccagccg gatcagcgac tacaacactc
    59401 atacggcgac gttcaaaatc aaaaggaatt tcatctactt tttgataatt gattgtatct
    59461 gtatctaaag tttctgctgc ttcatcaatg atagccacat ctaacaaatt tttcagccct
    59521 gtttgatagt agctgtttag ataagcatgg cgcaaaacac gatcatcttc atttccttca
    59581 caatccatat gatattcaag aatgattttg tcttgtgtca atgttcccgt tttatccgta
    59641 cataacacat cgatcgcacc aaaattctgg atagcattta aatttttgat gactgttcct
    59701 tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat tggaagcatt
    59761 tcaggcgtta aaccaactgc cacagataat ccaaaaagaa aagcttctaa ccaatctcct
    59821 tttgtaagcc cattgataac gataactgct ggagccatca atgacataaa tttgatcagc
    59881 agcatggatg ttttgtggat gcctaattca aaacttgatt ggatgggttt ttcagatagc
    59941 gtttctgcta catgcccaaa tagtgtgtca tttccgacag aaacaatgat tccttccgcg
    60001 cttccactga tgacattgct tcccataaag acaagatttt cataacttgt ttcactttgc
    60061 tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc tgtcaatgct
    60121 gcttgagaaa tgaatagatc ctttgcggac aataaacgca tatccgctgg tatcatatct
    60181 cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat ctctgtatac
    60241 tcgccatttc gtttgacagc tgccgttact ttcacaagtg atttcagttt ttcagctgct
    60301 tgattagaac gcacagactg gatcaacgtc atcgtcccac tgacgataac catagtgaag
    60361 ataatggcta caccaaagag atctttatct cctggagcgg ctaatacgta ttccgtaatg
    60421 aatgaaataa ttcctaatgc aatcaacact aatgtaaagg gtgtgatgta tgctttcaat
    60481 atttctataa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc ataagtttct
    60541 cttgaacgct cgatttgttc ttctgacaat ccatttggtg atgcatggaa caattcaaat
    60601 aattcttgct tcgtttttcc agcaaaagct gcatagattt tttgtgtatc tttctttttt
    60661 tccatctcga tctctccttt gtgtccaaaa gacagctgtt ctcgctgttt ttggtctttt
    60721 gctattcatc ggatcgatag agaaaatgct gaaaggggct gagaacagat agataatagc
    60781 cctggccttt cctctaaatc cttgtttttc gaatgactgt catccatttg ttctcatctc
    60841 ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt attttcccaa
    60901 ctcgctcacc tccctttttg ttttttcacc tatgggattt gcggcaacaa aaaaacctct
    60961 cgccaaatac tcagcgaaag gtttgaaaaa gcaaatcaaa aaaagcttct cagatctcat
    61021 cgttgagttt tgacactgta caacgtaaaa agcagatatc tgcttttagc taccttatga
    61081 aaagccttat ctcgacaatt cctgttctac ccattggcgt ctctcgacgt ttttgggcag
    61141 tagcctatgt ttgtatagga gcctcaccta acaaatccta ttcggttttc cctcgttaat
    61201 ataacaacta aaaaagagga tgtcaaatca attttccatg tttttcatac ttttttaaaa
    61261 tacacgcgtt atatttcctg tctcacgcag ttttatttgt ctcattttcc acctcccgct
    61321 ataatagaat tatagtagga aacgaggtgc tgttatggaa cgaacacttg ttattatcaa
    61381 gccagacggc gtaagacgcc atttggtcgg aagcatcatc caacgttttg aagcaaaggg
    61441 actggccatt gctgaaatga aatttgcaac gatgacacct gaactagcaa aagaacatta
    61501 tcagcacttg actgaacgta gtttttttga tgagcttatc gattacatga cttctggtcc
    61561 ggtcgtttat cttgtgctgg tcggcgagga agtgatcgat atcgtccgta agatggtcgg
    61621 agcaacaaaa ggggcggatg ccgttccagg tacgatccga ggagattatg cactgccggg
    61681 gactgaaaac atcatccatg cttctgattc ccgtgatgct gcaacaaaag agatcgcccg
    61741 ctttttcccg ccattcgata caaacaaacc gtctgaaaaa gcggcgaagt gatacaaatc
    61801 ctaaactgtt ggtctaactg ctgggtttag caatggccag cagttttttc tctaaagaat
    61861 tcctttgatt cttccctaat gtatattcga tcactgcgaa aaactcttgg tgattttccg
    61921 atttttcttc ccctaaagca gcagaaatca gtcgttggta ataatccgcc atcaatgttt
    61981 cataaatcac tgtacttctt tctttatgcg ctaaactccg tttcatatct tcattcacgc
    62041 tacgtgcctt ttttaagcga ccattttcta tataaaacaa agtcaagttc gtcaaaaaag
    62101 taaagagata ctgctcgtga ttattcagtt gcgcgatccg tcgcattctt tttttgattt
    62161 tcaaataatt attctcaata aaacgatcac tgaatatcca aggaaaatct gctagaatag
    62221 atacttcaaa ttgcccatac tctggtactt gcatcaaata ttcttcaatg atacgacatt
    62281 cataacgcaa aaattcaaat tccgtctcag atacttctgt ttttttggac atgtgcagat
    62341 tcaaaaccaa atagtacaga tcttctgttt tcttatagtg cctgcggtta ttagcaattt
    62401 ctttgtaagg atcaaagctg tcttcttgaa tccgatctag cattcgtctt tttttcttta
    62461 atttttttgg ctcatcaagc tgatttcgta tccattcata ttcggcaagt gtcatgttca
    62521 ttcgctctaa aaatgatttc aatgttccaa acgatacttt tttcactgaa tttgtctcga
    62581 tcccgactag cgaagtttta gaacagattc cttccgctaa ttgggtctgt gtcagctttt
    62641 tgttgactcg aagcttctta atcaattctc catcatacat aatagttctc cttttttaat
    62701 tgaaattaac ccttggcttt tccagaatgc ttgccaataa gaggaatgat tatttcaatt
    62761 taaattttcc gcattcaata aatagatacg ttaaaaataa aaaatacacg aaaaaaataa
    62821 aaaaatgttt ttctcataaa aaataccaac cttcaagcat gagactgctg tctaatccct
    62881 gaaggttggt ttgattgttt attgatcatt tttttactgc tccgcattgg ttcctgttac
    62941 tggatatagg atagtcgttg ttttattgcc gttttttgaa aagacactgg tcgaatgttc
    63001 acctttttgc ttttcgattg cttcattacg ttccgcttcg tttttatagt catatttttt
    63061 cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt cctgattcag
    63121 cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga tttcctcgtc
    63181 gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag cttttcctcc
    63241 taggacggtg tatttgggcg tcacgaagtt cccatttcga aaagcaacta cttgatcgtg
    63301 ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga ttcccaatag
    63361 atgtagtaat gtcggcaaaa catcgatttc tccaccatat tcatgattga ctgtcccttt
    63421 agtgtaacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt tgtcaaattc
    63481 atcccatgta tctggatctt taccaagagc acttgctaaa tctttattat ctgtattcga
    63541 gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac cagatttttt
    63601 caaatacgta aaaaattgtt ccaatgattg atccagataa tgggccgttc gaacataatt
    63661 atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt catccgtata
    63721 ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt gtagatgttc
    63781 aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac ctaacgtttc
    63841 atcggattca tcaaaataag atcgatcaaa gaaattgtca taccctaaat ttttatatac
    63901 atgatctcta ttccagaaac tagctacatt cccatggaaa acagcacttg tatagccttc
    63961 tgtttgacca agggtcgctg gtgctgcttg aaagacatta tctgacccaa gttctgtgaa
    64021 taatgagcct tgaggtaaac caaatgtgcc agtttccaac atgttttcag catcacttgt
    64081 ctttccctgt cctacttcat gaaagaaatt gtcaaaactg atcgttgctt gattctggaa
    64141 aatactattt aaaaacggcg tgacttcttg tccgtctacc ttcatattga tcaaaaactg
    64201 ttgaaaactt tctaaatgaa ggacaatcac attttttcct ttagcaatcc caaatgtttc
    64261 aggatttggc tctgcataat gtttctttgt atagtctaaa acttcatcaa ttccattact
    64321 gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat aaatagtgta
    64381 cgcatcgatt cctagatatt tgacaatata ggaacggtca aatgtccgct gtagcaattg
    64441 gggacggttg gcttcgctca acgctaaatt tcctgaaaaa atcaatcctg aaagacaggt
    64501 aacagcgata gccatcggtt tgctgacagg gttgcttttg ataggtattt tcttgacctt
    64561 caaccaaacc agtagcccaa tgaacacagc gatatccaac caatagatga catcatgggg
    64621 tttcatcaaa gaaaaggagc ttcctgacaa cccttgtgac actttcgaaa agcctaatac
    64681 agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca agtacaacaa
    64741 aagcgtattt aaaatatcaa tgatcaaaag aatggctaac gctggctttg gctttttaat
    64801 atacaaagaa aggccaaaaa acaataaggt agtcgcaatc gggttgatcc acaagataaa
    64861 gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa tgatcgtctt
    64921 cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc acaatttttt
    64981 aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt gctcctgaaa
    65041 aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc taaagattat
    65101 tccattcatt tttcacagca taaaatcgtt tcggtgtaag cggttaaaat agtaaggaga
    65161 taaaaatctt gtggtgaccg atttactcta tagtattcaa aagtagtttc tcggtattcg
    65221 tacaaaaaaa acatactaag attagagggg aacatcattg acgaatgatg ttctcctcta
    65281 ttttctattt ttagattata gtgaagagtg aaagaaagtt cgaacaaagc cttgaggact
    65341 acgagtccct atcaaaaacc gaacttcttt actgtcattt tagaatcagg tttacgtatg
    65401 ttggggccat gagcccgtgt ataggaaatt ggaatcgaaa tgaagcagta aagttctttc
    65461 aacacttaaa aaggaggaaa acaaatgcct tacattcttg cattagatgt ttcaatggga
    65521 cacagctaca gtgtcctata caaagacgac acctgcttat ttgaagacga gatcgaacac
    65581 actcaaagag gatttcatgc attactcgaa gagatccagc acttgcctga acgtccattg
    65641 attgtttttg aagcaacggg tatttattcc agaccagtcg aaaagttctt tcaagatcat
    65701 cacttttctt actgtttgtt aaacccgtta gaagcgaaaa aacagatgga agaaaacacc
    65761 ttacgcagtt ggaaaacaga taaatcggat gcccatcggc tcgcacagac tcatcttaaa
    65821 aatcaacgtc aaccaaaaga agtacaaagc aatttttact tagagatgcg cgatttagcc
    65881 cgtttttatc aagaaattga gaagaaaatc actcgactgc gcatggattt acacaactgc
    65941 cttcagttga cttttccaga gcttgaacag ttcttttcaa acaggctgac cccttacgca
    66001 ttgacattga tccgtctttt tccccatcca gattttgtat tggcatctac tcggacaaaa
    66061 ataaaaaata aactgatcaa cgaaacacgg gaaaaaatct ccgcgaatcg tgccgagcaa
    66121 aaggcagatc aaatcatcca ctatgcacag tgtgcctatc cagctgttga aaaagacagt
    66181 attcactgtc aaaaaacgat ctattacgcc gaacttctcc aagatttact cgaacaaaaa
    66241 aaagcactgg cgacacaaat gatcaaaaaa gccgaagcct ctccttgctt tttgttgtac
    66301 caaacgttcc ctgggattgg cgcactcaca gccgctttat tgctcggtga attaggagat
    66361 atcacacgat ttaaaaccca taaacaattg aatgcctttg tcggaatcga catccgacgg
    66421 tatcattcag gtaagtatac tggccaagac cgcatcaata agcgtggtaa tcccaaagca
    66481 cggaaaatta ttttcttcac cattcgaaat atgattcgcc agcaacgcgc agctcctaat
    66541 catattgtcg attattacta caaattaaaa aagcaaccta tccctaagaa agataaggtt
    66601 gccacagttg cttgtatgaa caagcttctg aaatgtatgc acgctatggt tagggcacat
    66661 acagagtacg actatgcgta cgcggtctca gtggaccatt aactaaacca cttacagtat
    66721 accacttctt tgcccagatc gaaaattttt tacgaacggg gctttattta gtgtgccttg
    66781 attttgtcgc tttcgtttac gagctatttt tcttgcacct tttacttgac taatcgtagg
    66841 aaagaggctg tgacaaaatt cttgtcgcag ctccctattt cttgatgcag gacagctttt
    66901 tcacaacctc tccactaaac ggtgttcaat cagctgacct tcggtattag ctcaaaaaac
    66961 ggcaaaatat gaggagctat tttcccgttt ttttctctaa tactcaggtc aaaccgctga
    67021 tttcacaacc tctgattcac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    67081 ttacaaaaac ttgagaagca cttttcgtaa atttcttctt atttcttgaa gctgaccact
    67141 tctgtcacaa cttctgtttt tcattgtcca taggtttgat attgtccttc tgatgactca
    67201 ttcgtaatga ttgaggcttg attttcttca tttcccgtta tcgtttgtcc actgccagaa
    67261 tctgaagggc ttagttctgt cgactcgttt ttttgagaag aagcagttgt tttcttgact
    67321 tgttcgttgg tctttttcca ttccgctagt cgatcggcca ccactttttt gtccactttt
    67381 tcagaataag ctgtaaccac ttttccattt ttcaaacgtg ctaccttgtc gccatcatga
    67441 gtcaaaagca aaacgaccaa atgtttgtct ttgaattgat attgtctgag agaaatcgca
    67501 ctaagtacgt catcgatatt gataaattga ttttcttctt ttccttttgg caaatagatg
    67561 tccaattcac ctttttcgta attggcagtt aatttatatt cctcccacca ataagttgag
    67621 aattcttttt tgaaattatc tactgattct ttgcttgcta cgattttttc tgtttctttc
    67681 tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa
    67741 aaaccgaata accaaaagag aagaaacggt ctttttttcc tcatgccttc ctccactcct
    67801 atttaatttt gactattcta ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa
    67861 aaagatcgta aataattgaa cttctttttc ctgaagcggt aagaacggga attttcatac
    67921 attctctctt ggctattctt taagcttcaa atccatttct gtctgataca tctttgaacc
    67981 aatgacccga tttcttgatc gttctttttc catcttgtgc taaatctact gaaataaaac
    68041 cgtaacgatt cttataggca ttggtccacg accagttatc catgcatgtc cacatatgat
    68101 aacctaaaca attggcacct tcttgaatgg cttgatggac atatttcaga tgagattgaa
    68161 caaattcgat ccgataatca tcgtggatca taccatcttc cttgataaat cgtgcttcat
    68221 tttctacacc catgccgttt tcagaaataa aacagtcgat attcccataa tgatctcgta
    68281 cattgatcaa aatatcatag attccttttt catagatctc ccatccgcga taaggattca
    68341 ttttacgacc aggcatttca taattgtcga agtaatcctc aggcatagga ccgtttttag
    68401 tatcgattgg tgtttctttt gcttttattc ggcgtggttg gtaataatta attcccaaaa
    68461 gatctactgt gttttcttta atgatcgtca aatcatctgg atgaatctcc ggcattagat
    68521 cgagttcttt tattctctca atcaattcac tgggaaactc gccttttaca gctggatcaa
    68581 ggaatgaacg attgaagaac gcatctgcta ttcgtgctgc tttaacatct tcgggatttg
    68641 attcgtctct tggataactc ggtgtcaaat ttaagatgat tccgatcaca ccgctttgat
    68701 ccatttcatg gtaagctttg atggctaatg ctgaagccaa cgcttcatga taaccaactt
    68761 ggattgcctt tttcatatct gattcttccg gataatgcca tcgatacaaa tagcctgcct
    68821 cgactggaac gattggttca ttatgggtaa accatttttt tactttcgag ccaaacaatt
    68881 gaaaacaact ttttgcgtat ttttcatagg cttcaaccgt ttctcggttc aaccatccgc
    68941 ctttttcttg cagatacatc ggcatatcga aatgatacag attaacaaag ggttcgatac
    69001 cgttttcaat caactcatca aaatagtgat gatagaaacg aacggcttct tcgttgattg
    69061 ttccagattc atcaggtatc aaacggctcc attggataga ggttcgaaat gaattatgcc
    69121 cagtctgttt catcaactgt acatcctctt gatacttcgt ataaacttgc gaggtttttt
    69181 caggtcctac ttgatcaaag aacttttgcg gttcgttttt gtaccaataa tcaaaaatat
    69241 tctctccttt tccatcgcct gaaattcttc cttctgtttg tggaccactt gctgcagagc
    69301 cccaccaaaa attttttgga aattgatatc ttttcatcaa agcacctctt tttctttttt
    69361 agttgattcc atgtatgata ttttccatca caatcacttt ttcattttct tcctcttctt
    69421 ttttgattcg tttcaacagc tgttctgctg ctcgttcccc gattttatct gttggtggcg
    69481 tgatcgtgat ttttggaagg atactcagat cgtttttatc taaataatca tagtgactga
    69541 acgcaatatc cttcccgatt ctgacacctt cttgattgaa acattcgatg gcaccaatcg
    69601 acatgttgta attgctgaca aaaatagcag ttgccccttt ttctaataat gcttttgccc
    69661 cgtaatagcc gctttttcta gaatagtctc ctgtaaagat ctctaaatgg atagacggat
    69721 ccttttgtac cgcttctttg gctccagcta aacgttctct tgctacgtaa tctgtttgag
    69781 cggcagcaat aaatcctatt gttttatgtc cttgtccgcc atgatgagca atcacttttg
    69841 ctacactttc tcgatcattc actactattg tatcaatacc tgctggccca cccggacaat
    69901 tcaatgcgac aactgggata ctcatttttg tcagcatttc aaatccagac cactcttctt
    69961 ctgataagaa gacgatcaat ccatcgatcg catgcttcat caggtattcg attttttgtt
    70021 cgattttttc tggctgatcg gcaaacccgc ttaataataa gctataccca ttttcttcag
    70081 ctactctttc gatccctgaa acaacctccg aaccaaatcg gttagatatc ccgtttatca
    70141 acagtccaat agagaaactt cgattattct tcaatccttt agcaatgatg ttttctttat
    70201 agtccaacgc tgaaatcgct tctgatattt tttccatatt cttttccttc agctgctgac
    70261 cgtttaaata gcgggaaact gttcccactg aaacaccagc catcttagcc acgtcacgga
    70321 tcgatgccat acattttcac tcctgccagt tacatttgat ttctttttct tgttgttcga
    70381 ccatgatgac cgctttcttt gcataatgcc gcttgtcatc aatatccata taactaaagt
    70441 cttcagaagt gagttcaaaa acaagcagtc tttcctcttg agaccgcaat tcatctttac
    70501 gaaaagcttt caataatttc gttctttgga tgacaacgcc gtcaattaat cggacataga
    70561 caagagtact tgttttacct gaaacacttc ctgtattttt aactttaatt gatattatta
    70621 ctttgtcaat atccgttttg acttccatct gactgtattc aaatgttgta tagcttagac
    70681 catgtccaaa aggataaaat ggacttccgc ttaaatcata ataattctct tgtttagaag
    70741 cagcacgctg atagtaataa acaggtagtt gccgactatt tctaggataa gacaaactca
    70801 atcgtccgct tgggttattt cttccagaaa tcatctgcgc aatggctttt ccaccctcct
    70861 gtccaggaaa ccatgctgtt gcaatggcat tcgtaaatga ctcgattttc atcaaatcgt
    70921 agggtcttcc ttgcacaagt atcgtgatga ttggcttgcc tagttgatac aattgttcaa
    70981 tcagctgttc ttggtatcca cctaaagaaa gagaagcaac atctacattt tctccagaat
    71041 ccatgttgat ccctttcgat gaaactgccc cgttacgaag aaattccata tcaaagttcc
    71101 gcgcacttga tccacctaga actgcaatga tcacatctga gttttgagca agggtcaccg
    71161 cttctaataa tttttcttcc tgatcctcct gatttcttac ttcacatcct tgtgcataag
    71221 aaacctttga ttggggaaac tctgttttta tctcttgtac tatctttttt ttcaaacggt
    71281 cttctgtttg tggtgcagag tagtctccca acaagtggta aacatcgtga gcatttggac
    71341 ctataacagc aatgtttttt acttgttgat ccaacggcaa tataccgtca ttttttagta
    71401 aagtgatact ttcttttgct gtctctaaat ttaagtgctc tgattcctcc atgagatgat
    71461 cccaatactc ctttggatcg tttgcaaatg gctgttcaaa taaacctaat aaaaatttga
    71521 cagataaaac tcggcgaact gcttgatcta gtagcttttc atctacgact tgattatcaa
    71581 tggcttctgc aatcttggta taggtctcat cccacaaact gagatcgatt cctgccgcta
    71641 atgcatatgc tgcagcagtc tttttatctg taaaaacatc ggataaacga tccaaagcca
    71701 caccgtctgc cataacgatt ccttgataac cgatctcttt tcttaatgtt tgctcaaaaa
    71761 gcgctcgatt gatatgacaa ggtatcccgt cgatgtcatt ataagcagcc atgacaccta
    71821 ctgcatttct tgtactcttc aataatggtc cgtaaatatc ggagaactct ctgcgtccaa
    71881 tcgttactgt gcctgaatta tgaccgccta aagcttctcc ttgtgcgata cagtgcttta
    71941 acacaacacc gatctgatcc ttcttcttat tgatttcctc tactgtctga tcaagaaaag
    72001 aaactttttc tgaaataatc tctccttgga accctcgaac gaccgcttca gaaaaacgag
    72061 ctgagagaat cggatcttcc ccaaagcact cttctgtcct tccccaacga ggatcttttg
    72121 ccagatccaa agtcgacacc agcgctaaat gaacaccttt agcagccagt tcttttgcca
    72181 tcaatcgact cgattgttct aataatgcta cattgaaagt atttccacgg ccgatattcg
    72241 ttggataaga aatcccatcc aagccttgat gaccatgtgg acattcttct acaagcaaaa
    72301 ctggaatttt ccatcgcgaa tgctgtagta catattcttg gactaggtta gccaccttcc
    72361 agctttcttt tgcaggaatc ccattcttat aatctgtttt agaccacgga tcagagcgaa
    72421 acagaccgta taaggcaccc agaccgcctc cccatttaac atgttctttt aacttttctg
    72481 ttaattcgat cttgtctccg tttttttcat aacattccca accatacaaa tgctggttta
    72541 cctgtcctac tttttcagca atattcatcc tcgaaagtaa atcttctact ctttggcgga
    72601 tggttaactt tgggttttga tacgggtaat cgtacacctt aatatcctcc ttctgccaat
    72661 tttgccttat tagcggctaa tacaaatggc aggaataaaa atacatctag aatgataagt
    72721 attactgtta agactgctgc tcgtatatcg cctcctgtag caataaaaga ttgtataacc
    72781 ggtggagtga tccaaggtgc agcagcaact gttttactga tgattccaag acttgtggct
    72841 gcgtaggcaa taaccaagtt aatagatggt ataatagcgc atggaatcat atatattgga
    72901 ttcatgacta ctggtaatcc aaacataatt ggttccgtaa tattaaataa acaagtagtc
    72961 agaccaaatt tagcgattga tcgctgttct tgacgtttag aagcccacaa gatagcaata
    73021 actaacggta aaatacaccc gccacctccg atcaatccaa atgcattggt aaagggctcc
    73081 gtaataatat taggaatttc ttttcctact gaaaaagcat ccatgttttc ctgaatagac
    73141 tgtaacaaag gtgcttgccg tataggagcc aaaatactcg aaccatgcat accaaacacc
    73201 cataagaaat cagagaaaaa ctgtaaaaat aagatgcctg ggacggataa aacaaaagct
    73261 ttcaatggtg tctgaatcgc taattcaatt aattgcggaa cattcaaacc agtcgtatgg
    73321 tttaaagtaa aaacgacaac tgaaaaaaat aaaatgacaa ctatttctgg tactaaagaa
    73381 ttaaatgacg cagctactgc cggtggaact ccttctggca tcctgatttt aaaactcttc
    73441 attttcgcta atttgataaa taattctgta ctgacaaggg cagcaatcat cgccgttgct
    73501 aagccagacg aagaagtcag actttgtaag aaaacccctg tagcttctgc tgtatttccg
    73561 tccatcaaag tgactgtact tgataaagac atcaaaataa acattacggc tgtacttaga
    73621 attcctgcat gaacaggact aaatgatgga tcatcaatca cgcctgtttg gatatagtgg
    73681 gttgccaaat tccagccgat attgtaacac acgataatag ctaaaatact catcgtacca
    73741 ttattcacga ttgaagcaag ttcagccaat ggacttagat caagtattct ttggactgtt
    73801 gcatttgaaa aaatcagagc attcagcaaa accatgacag aagcagccat tactaacggc
    73861 atagtaacga taaaactgtc tcgaatggcc agcaagtgtc gctggctact catcttagat
    73921 acgattggta gcattttttc agatagtctg tctacgactt tcattatttc atctcctaga
    73981 ttctaaaatt gaaccggttc atttttaata tagcactttt tttaaagatt gcaacacatt
    74041 tttttaaaac gctttctaaa ggtggtggat tttttatgac ttttgaaaaa ttattctatt
    74101 taatggtatt ttgtgaattt tttctcttcc atccgtttga agttttactt tccaatggta
    74161 taatttcttt atcctatcaa atgggggaag tattttgaag aaattaatcg tactatttat
    74221 tttgtctgcg ggacttttga cattgactgg ctgtgatttg tttggcggct ctgaccaatc
    74281 ctcggacgaa acaaccgatt cttctgtctt aaaagcggaa aaagcagata tcaccaacag
    74341 tgtatatacg ctaaaagtac gtaaagatat ggatttatcc gcgggacagt tgacatttac
    74401 agataaaaaa atggaatgga cacgcacgta tcagacacaa ggagctgatt cttctgaatc
    74461 atctgaagaa aaaacagtgc tgacagatat caagattact accaaagatg acgaatatat
    74521 tatttcagga aaagaaaata aaaaagaagc aacagtcaca tttaccaaaa tcggtaatta
    74581 ccgtttgaag gataaggatg gcaacatcta cagtctatag tgccatctgt tggatcttct
    74641 tttgtttctc cccttctttt ctgctatagt aaagaagcaa gatgactgta aaggagacga
    74701 aatggatgat cgatagtcct gattattgta catgtaataa caacaaaaat atccatacag
    74761 aatttaatga tcaaggaagt ttgagtatat gcgacaattg ccacaaacta gtcaaagatt
    74821 cttttaaatc gtttgaatct tatcctgtca aaaattatgt aaaaagcaaa agaagataag
    74881 caaaatcaag caaaactcac tatggctgat ttttatgaac ttcaaactca gcttcttcct
    74941 ttgatcaagc ttcaaaaact ggaatcagta aaacaaacag tggatcattt cagttcacaa
    75001 atcaaagagt aacgttttat ttatttcaca tctttgaata taaaataacc aaacgataga
    75061 aagacttatt ctacatcacg tagtaatttt t
//
LOCUS       DADXTH010000004        73375 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.004, whole genome shotgun sequence.
ACCESSION   DADXTH010000004 DADXTH010000000
VERSION     DADXTH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.004"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..230)
                     /locus_tag="IWI97_000234"
     CDS             complement(<1..230)
                     /locus_tag="IWI97_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461776.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ"
     gene            713..928
                     /locus_tag="IWI97_000235"
     CDS             713..928
                     /locus_tag="IWI97_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBC2461777.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            1789..2748
                     /locus_tag="IWI97_000236"
                     /pseudo
     CDS             1789..2748
                     /locus_tag="IWI97_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            3279..3689
                     /locus_tag="IWI97_000237"
     CDS             3279..3689
                     /locus_tag="IWI97_000237"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBC2461778.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            3682..4137
                     /locus_tag="IWI97_000238"
     CDS             3682..4137
                     /locus_tag="IWI97_000238"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2461779.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            4154..4885
                     /locus_tag="IWI97_000239"
     CDS             4154..4885
                     /locus_tag="IWI97_000239"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2461780.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            4902..5714
                     /locus_tag="IWI97_000240"
     CDS             4902..5714
                     /locus_tag="IWI97_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBC2461781.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            5862..7049
                     /locus_tag="IWI97_000241"
     CDS             5862..7049
                     /locus_tag="IWI97_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBC2461782.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(7385..7684)
                     /locus_tag="IWI97_000242"
     CDS             complement(7385..7684)
                     /locus_tag="IWI97_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBC2461783.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            8035..8667
                     /locus_tag="IWI97_000243"
     CDS             8035..8667
                     /locus_tag="IWI97_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBC2461784.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            8790..9428
                     /locus_tag="IWI97_000244"
     CDS             8790..9428
                     /locus_tag="IWI97_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBC2461785.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(9547..9619)
                     /locus_tag="IWI97_000245"
     tRNA            complement(9547..9619)
                     /locus_tag="IWI97_000245"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9584..9586),aa:Thr,seq:cgt)
     gene            9740..9982
                     /locus_tag="IWI97_000246"
     CDS             9740..9982
                     /locus_tag="IWI97_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461786.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(10121..10750)
                     /gene="upp"
                     /locus_tag="IWI97_000247"
     CDS             complement(10121..10750)
                     /gene="upp"
                     /locus_tag="IWI97_000247"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBC2461787.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(10884..11831)
                     /locus_tag="IWI97_000248"
     CDS             complement(10884..11831)
                     /locus_tag="IWI97_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2461788.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(11984..13228)
                     /locus_tag="IWI97_000249"
     CDS             complement(11984..13228)
                     /locus_tag="IWI97_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBC2461789.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(13403..14428)
                     /locus_tag="IWI97_000250"
     CDS             complement(13403..14428)
                     /locus_tag="IWI97_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBC2461790.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(14450..14629)
                     /locus_tag="IWI97_000251"
     CDS             complement(14450..14629)
                     /locus_tag="IWI97_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461791.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(14622..15461)
                     /gene="prmC"
                     /locus_tag="IWI97_000252"
     CDS             complement(14622..15461)
                     /gene="prmC"
                     /locus_tag="IWI97_000252"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBC2461792.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(15454..16527)
                     /gene="prfA"
                     /locus_tag="IWI97_000253"
     CDS             complement(15454..16527)
                     /gene="prfA"
                     /locus_tag="IWI97_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBC2461793.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(16530..17114)
                     /locus_tag="IWI97_000254"
     CDS             complement(16530..17114)
                     /locus_tag="IWI97_000254"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBC2461794.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(17220..17689)
                     /locus_tag="IWI97_000255"
                     /pseudo
     CDS             complement(17220..17689)
                     /locus_tag="IWI97_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            17795..18397
                     /locus_tag="IWI97_000256"
     CDS             17795..18397
                     /locus_tag="IWI97_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBC2461795.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(18501..19070)
                     /gene="lepB"
                     /locus_tag="IWI97_000257"
     CDS             complement(18501..19070)
                     /gene="lepB"
                     /locus_tag="IWI97_000257"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBC2461796.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(19300..20067)
                     /locus_tag="IWI97_000258"
     CDS             complement(19300..20067)
                     /locus_tag="IWI97_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461797.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(20064..20543)
                     /locus_tag="IWI97_000259"
     CDS             complement(20064..20543)
                     /locus_tag="IWI97_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBC2461798.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(20668..21405)
                     /locus_tag="IWI97_000260"
     CDS             complement(20668..21405)
                     /locus_tag="IWI97_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBC2461799.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            21566..22966
                     /locus_tag="IWI97_000261"
     CDS             21566..22966
                     /locus_tag="IWI97_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBC2461800.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            23189..24541
                     /locus_tag="IWI97_000262"
     CDS             23189..24541
                     /locus_tag="IWI97_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBC2461801.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            24534..25232
                     /locus_tag="IWI97_000263"
     CDS             24534..25232
                     /locus_tag="IWI97_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBC2461802.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(25294..26445)
                     /locus_tag="IWI97_000264"
     CDS             complement(25294..26445)
                     /locus_tag="IWI97_000264"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBC2461803.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(26458..26895)
                     /locus_tag="IWI97_000265"
     CDS             complement(26458..26895)
                     /locus_tag="IWI97_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2461804.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(26910..28676)
                     /locus_tag="IWI97_000266"
     CDS             complement(26910..28676)
                     /locus_tag="IWI97_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBC2461805.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            28940..29617
                     /locus_tag="IWI97_000267"
     CDS             28940..29617
                     /locus_tag="IWI97_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2461806.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            29620..30747
                     /locus_tag="IWI97_000268"
     CDS             29620..30747
                     /locus_tag="IWI97_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2461807.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(30947..31387)
                     /locus_tag="IWI97_000269"
     CDS             complement(30947..31387)
                     /locus_tag="IWI97_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2461808.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(31398..33431)
                     /locus_tag="IWI97_000270"
     CDS             complement(31398..33431)
                     /locus_tag="IWI97_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBC2461809.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(33433..35136)
                     /locus_tag="IWI97_000271"
     CDS             complement(33433..35136)
                     /locus_tag="IWI97_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2461810.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(35230..36180)
                     /gene="manA"
                     /locus_tag="IWI97_000272"
     CDS             complement(35230..36180)
                     /gene="manA"
                     /locus_tag="IWI97_000272"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBC2461811.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(36219..37244)
                     /locus_tag="IWI97_000273"
     CDS             complement(36219..37244)
                     /locus_tag="IWI97_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBC2461812.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(37453..38322)
                     /locus_tag="IWI97_000274"
     CDS             complement(37453..38322)
                     /locus_tag="IWI97_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBC2461813.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(39026..40918)
                     /locus_tag="IWI97_000275"
     CDS             complement(39026..40918)
                     /locus_tag="IWI97_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2461814.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(40915..42642)
                     /locus_tag="IWI97_000276"
     CDS             complement(40915..42642)
                     /locus_tag="IWI97_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2461815.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(42658..43308)
                     /locus_tag="IWI97_000277"
     CDS             complement(42658..43308)
                     /locus_tag="IWI97_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBC2461816.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            43512..43808
                     /locus_tag="IWI97_000278"
     CDS             43512..43808
                     /locus_tag="IWI97_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBC2461817.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            44000..44620
                     /locus_tag="IWI97_000279"
     CDS             44000..44620
                     /locus_tag="IWI97_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBC2461818.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(44712..45878)
                     /locus_tag="IWI97_000280"
     CDS             complement(44712..45878)
                     /locus_tag="IWI97_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2461819.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(45890..46243)
                     /locus_tag="IWI97_000281"
     CDS             complement(45890..46243)
                     /locus_tag="IWI97_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBC2461820.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            46371..47393
                     /locus_tag="IWI97_000282"
     CDS             46371..47393
                     /locus_tag="IWI97_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBC2461821.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            47412..47573
                     /locus_tag="IWI97_000283"
     CDS             47412..47573
                     /locus_tag="IWI97_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461822.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(47832..48266)
                     /locus_tag="IWI97_000284"
     CDS             complement(47832..48266)
                     /locus_tag="IWI97_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461823.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(48376..50235)
                     /locus_tag="IWI97_000285"
     CDS             complement(48376..50235)
                     /locus_tag="IWI97_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBC2461824.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(50353..51513)
                     /locus_tag="IWI97_000286"
     CDS             complement(50353..51513)
                     /locus_tag="IWI97_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBC2461825.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(51820..53244)
                     /gene="araA"
                     /locus_tag="IWI97_000287"
     CDS             complement(51820..53244)
                     /gene="araA"
                     /locus_tag="IWI97_000287"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBC2461826.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(53260..53958)
                     /locus_tag="IWI97_000288"
     CDS             complement(53260..53958)
                     /locus_tag="IWI97_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBC2461827.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(53915..55576)
                     /locus_tag="IWI97_000289"
     CDS             complement(53915..55576)
                     /locus_tag="IWI97_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBC2461828.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            55794..57194
                     /locus_tag="IWI97_000290"
     CDS             55794..57194
                     /locus_tag="IWI97_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBC2461829.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(57446..58525)
                     /locus_tag="IWI97_000291"
     CDS             complement(57446..58525)
                     /locus_tag="IWI97_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2461830.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(58611..59570)
                     /locus_tag="IWI97_000292"
     CDS             complement(58611..59570)
                     /locus_tag="IWI97_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBC2461831.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(59582..61093)
                     /locus_tag="IWI97_000293"
     CDS             complement(59582..61093)
                     /locus_tag="IWI97_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBC2461832.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(61115..61678)
                     /locus_tag="IWI97_000294"
     CDS             complement(61115..61678)
                     /locus_tag="IWI97_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBC2461833.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(61684..62547)
                     /locus_tag="IWI97_000295"
     CDS             complement(61684..62547)
                     /locus_tag="IWI97_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2461834.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(62561..63457)
                     /locus_tag="IWI97_000296"
     CDS             complement(62561..63457)
                     /locus_tag="IWI97_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2461835.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(63632..64921)
                     /locus_tag="IWI97_000297"
     CDS             complement(63632..64921)
                     /locus_tag="IWI97_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2461836.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(64893..66410)
                     /locus_tag="IWI97_000298"
     CDS             complement(64893..66410)
                     /locus_tag="IWI97_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBC2461837.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            66608..67498
                     /locus_tag="IWI97_000299"
     CDS             66608..67498
                     /locus_tag="IWI97_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBC2461838.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(67571..68395)
                     /gene="nadE"
                     /locus_tag="IWI97_000300"
     CDS             complement(67571..68395)
                     /gene="nadE"
                     /locus_tag="IWI97_000300"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBC2461839.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(68407..69879)
                     /locus_tag="IWI97_000301"
     CDS             complement(68407..69879)
                     /locus_tag="IWI97_000301"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBC2461840.1"
                     /translation="MNNTYPDESLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(70516..70878)
                     /locus_tag="IWI97_000302"
     CDS             complement(70516..70878)
                     /locus_tag="IWI97_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBC2461841.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            71101..71853
                     /locus_tag="IWI97_000303"
     CDS             71101..71853
                     /locus_tag="IWI97_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2461842.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(71993..72916)
                     /locus_tag="IWI97_000304"
     CDS             complement(71993..72916)
                     /locus_tag="IWI97_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2461843.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(72931..73203)
                     /locus_tag="IWI97_000305"
     CDS             complement(72931..73203)
                     /locus_tag="IWI97_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461844.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        21498 a        14825 c        12790 g        24262 t
ORIGIN      
        1 atttgctccg tattatctac aaccgcatat tcatctaaag gcttccacaa aacaggcttt
       61 cccggtaatt tctcgacctt tttatcttcc actaatctcg tcaaataatg gctggcattc
      121 tgacgagaga tacctagatt cttcgaaatt ttctcggcag tgacagaaga catattccct
      181 gttagtcgta tcagctcatc aaaaactcga tctttagcgc tctttttcat tttcccctcc
      241 agttcaacgt gtctagaagc ataataaact atcggctaca atatgtctat tctatatttc
      301 tgttccaatt ttactttgaa acaaaaataa tcgagcttaa actgatatta gttataaaac
      361 tatagttaga ttaccaatct tcctctcata caataaactt ctctcacctg catccaacct
      421 acttatgtga aagaataact gccgattatt tatctttttt acagttactt tcgaacataa
      481 aaaaatcacc ttcaaatact agaaaaacta ctatttgatg gtggtttaga acaatttccg
      541 ttgattcagt atcgtctggt cccacatcct ctgtctcatt aatcacaata gcatactcca
      601 aaggataaag ctggttgata tctcaatgcc tttcacacct atcaattttg tcacacttat
      661 cttttcctag gttctattct atataatcta gaaaaacaaa gaaggagaca ccatgaaaaa
      721 attcattatt ccttgtatat ttcttttctt gagtattttg ttattttcaa cttccatcct
      781 agtcggttct tacctcgatt caagcggaat gctgattgaa ccagcgttct tttgtgttcc
      841 gttgggctac ttaacattag gacttgcttt ctttactgcc ttatacagtt tcgtcaaaca
      901 aagattattg aagaaatact cggcttgatt tgcacgtaaa tcctctgttg aagaaaagtt
      961 agttttttca acaacgaacc ttcgagtatt aagaatggat gttgatgtag acatcaaacg
     1021 tctgcataaa cattcatttt attcctccat taccaacaat tattcccaat cgccaaaaga
     1081 agcttttttc ggaattacct tctccatttt ttcacctgct ggttatattg tcttttgtaa
     1141 taccgatagt cctcttcaat cattttcaat atgcgaaaag aaccaatttt aacatggcac
     1201 tcaaatcaca attcctaggg cggccaaaaa gatacggctc gttgatttca agggtttcaa
     1261 cgagttcatt gacaaaccca gcggtgctat cttcttttgg aataagaagt tgtgatatct
     1321 aaagtgagtt gattcatgtt ataatttgta tgcatgattt tgatcctttc aagggttgaa
     1381 tgatttagag tgcgagtatc ctctcgtact cttttatttt acctaaaaag ctccagaaat
     1441 gacgaatgac tcgtcatttc tagagctttt ttcagagaat tatgtcacaa tctctttttg
     1501 ttatattgat attctatatc tttttttatt ggttttattc ccttgttcgt tatagactaa
     1561 aatccttctt tcagcattat tcaaatcaaa tggcaacata atagagaaat ttatagtatg
     1621 tataatcatc tgaattcaat caaatcaacc ggagtcatat acggagtcaa actatcccac
     1681 tgagagttta ttaaacgcag attgtaaaat taagctagac aactaaaaaa gtcatttgtg
     1741 ctacactcaa aatagttccg accaaagaat tataaggagt gaagacaaat gacctatacc
     1801 catcttacaa caaatgaact cgtgatgata gaagcctatt tcaatatgca tcaatctgtt
     1861 accgtagtcg ctaagacctc aaatcgttcc agacagacca tctacaatgt gtacaacttc
     1921 ctgaaacaag gccaatctat ccatgactat taccaacaat atcaaaaaaa taaaacacgg
     1981 tgcggccgac gacccatttg tttgccagca gaacaagatg cgtatatccg aaacaaggtg
     2041 gcgcaaggat ggacacccga tgtgattatc ggtcgtgctg agttttcaat ttcatgttct
     2101 gttcgaaccc tttatcgcaa gtttaaaaca aactaatttg attgctcgaa actacccatg
     2161 aaaggaaaac ggaaacccaa cgggcataaa gaacgtcgag ggaaacaagc cttcaaacgg
     2221 aatatttcag aacgtctcac ggactatcct gaatttcaac aggaatttgg acatttggaa
     2281 ggcgacacta ttgtgggtgt ccatcataaa agtgctgtca tcacgctggt tgagcgcttg
     2341 tcaaaagtga ttattacttt aaaacccgaa ggacggaaag cactagatat tgaaaaagtg
     2401 acaactgact ggctacaaaa gcttccccga aacttgttca aatccattac ctttgattgc
     2461 ggaaaagaat tttcgaattg gaaaaacatg agtaatgcta acgatatttc tatttatttt
     2521 gcagatcctg gaacgctgtc acaacgggga ttaaatgaac attctaatgg attgttacgt
     2581 aaagacggac ttcctaatac aatggatttc aatcaggttg accaaaaatt tgtggcggcg
     2641 gttgcctcca aaagaaatca tataccaagg aaatctttaa attatcgaac atcgttagaa
     2701 gtatttttga gttacataag tgaggagcaa ttgtctaact taatttgaca tttagaaaat
     2761 aataaaccat atcccatcta ttcaaataat attttaccga ttgaaagact tgctaatcaa
     2821 agcagaatag tttatataga taaattattt tctttaaact tatactcatt tttagatatt
     2881 ttataagaat acttcatgtc ttcgccagta atataaagaa catctgtctc tccatttgga
     2941 cttgggttat ctattttggg tgtaacatac tatacataca accgatacat aaaagatttg
     3001 acatctggac aaaacacaat gctttacttc atttttatag gctttgttta caccatcaca
     3061 aaaatcacct cctaatttac aaattcatta aacaaacaga aataaaaaat caccacgata
     3121 gccgcgttca aaaaaattta caaaagagaa ttttagttaa agtttttaga ttcgccccac
     3181 taattaccaa aggtatttta tttattctta atacgaaata atttattgac atttagatta
     3241 ccaatggtat attatttata aataaatgga ggtggattat gtacacaatt attcttgcaa
     3301 gccacaaaaa tttagcgaaa ggaatgaaag agacagtcga atatatcctt ggccctgcac
     3361 cattcataaa agtaatgact gcatacacag acgataacta tgatattgaa aaagatacga
     3421 atattcttct cgatggaact gataaagtcc ttgtcatcac tgatttactt ggtggtagcg
     3481 taaataatca ttggatgaat tatgtttacg aaaaaaaatt aacaaaaaaa attacagtaa
     3541 ttgcaggtat gactttgtcc ttaattatgg aattaagtat gaacattgaa gattataaat
     3601 tgagagaaaa aatttccatg attattgctg aaagccaaaa gagcattata aactgttcag
     3661 aattaatgga ggtagaagat aatgattaaa ctattaagaa ttgacgatcg cctgatccac
     3721 ggtcaagttg caatggcttg gactaagtcc gtatctgctg actacattat tgtagttgat
     3781 gacgcctctg ctaatgacaa aatgaaacaa atgattttat cattagcaaa acctgtatca
     3841 acgggactag atattattac ttcagcagat tttttaagta cctttgaaaa aaggaaaaat
     3901 caaaatttga tgatagtcac tagttcccct aaaagcgcat ttgaaattgt aaacgctatc
     3961 ccaggggaag tcgattcaat taatatcggt ggtttacggt acgccgaggg aaaagagaaa
     4021 gttaacgagt atgtggctat cacatcagaa gacaaaaact acttagcaaa attaaaggag
     4081 tccggcttca aattaattat gcaggctact ccaactggaa aagcgaagat ttattagaag
     4141 gtggggaata agaatgattg aatgcatttt aattggcata gttgggtatt taacgacaat
     4201 agatgaaaga tattttgggg catctatgat gaatcgaccg ataatagttg gacctgttgt
     4261 tggacttatc ttaggggatc tccatcaagg catcttgata ggagcagcat tagaagcaat
     4321 gtttattggc attgtaacga ttggtgctgc actaccacca gatgtaggtg tagctggtac
     4381 tattgcaacg gctctagcaa taaaaagtgg agcaggtgct gatgtagctg ttacattggc
     4441 gatgcctttc gctgtattgg cacaaggatt aaacatgttg gcttttacga ttaactcctt
     4501 taccgtttca caaggtagaa aagcaattga aaaccgcaaa ttatcatctt ttcagaagtg
     4561 gcattttttt ccattaatcg ttcgcttacc ttccgcagta cttacatttt tagtagtata
     4621 ttttggtgcc aatgctacag aaagttttgt aaatattatg ccagaaaaat taatgaccgg
     4681 tttcaccgta gcttctggtt tactccccgc tgttggattt gccatgctaa ttcaaggaat
     4741 gattgacaaa aaatcagttc cttatttttt cattggatac cttttagcgg catatttagg
     4801 agtccccgta gttggtgtag ctgttgctgc aattatggtt gctttattag ttatccaaac
     4861 taaacgcaat cctgtaaata tttaaaggga gagtgataat tatgggagat tctaaattaa
     4921 ctaaaaaaga aataaacaag gttttctggc gttcatttgc tttacaggga tccgagtcat
     4981 acactgatat gcaaggaatg ggatatagtt tttctatatt acctgcttta aacaaaatat
     5041 atcaagggaa tgatgaaaaa tattatgaag ctgtttaccg aaatacggaa ttttttaata
     5101 caacgcctca tatttcaacc tttattatgg gtatgtcatt agcacttgaa gaagaaaatg
     5161 caaaaacaga tcaatttgaa actgaaacaa tttcatcgat aaaagctgca ttgatggggc
     5221 ctgtagctgg tattggtgat agtttttact ggggaacatt tcgcgttatt gctgctggca
     5281 tcggcttatc tttagctgcc caaggaaaca ttttaggtcc aatattgtat ttttcaattt
     5341 atacgctgtt acattttctt ggcagatatg gctgcttacg tattggatat cggatgggag
     5401 caaacgccat tgaaaaagca tttgagaaca attaccttga aagtttctcg ttagcagcat
     5461 ctatcgtagg tttaatgtca attggagcca tgacctcatc tctggtacgc tttactattc
     5521 ccgccgtatt taaatcaaaa ggtggagaaa taaatgttca gcaaatgctc gatgatattt
     5581 ttccaggaat tctctcattg gggctaacat tctttgttta ctttctatta aagaaaaaag
     5641 taaaggttct ttggattata cttgctatct tcgtctttag tattgttgga gtttatattg
     5701 gattattagg ttaattaatc cagaataaaa ctagatcaga catcaactta aacgattacg
     5761 tccatataat tgtgtaaaag tgaagcatca aaaaagccat tgatctcagt taggagtaca
     5821 atgttttttg cagaaaacat actcgaatag gactgatatc aatgactcaa gtacatttta
     5881 cttttgaaag cgaagaaatt caagcaatta taaacgaaag cggtgcgaat gataccgcaa
     5941 aaacacttat gactatcatg tttaatcagc tgatggaaga acaacgaaat caatatattc
     6001 aggcaaatgc ctatgaacgt tcagaagaac ggcaatcaca aagaaatggt tactacgatc
     6061 gttcgtttac aactcgaatt ggtacgttag aacttcatgt tccacgaacc agagacggta
     6121 agtttagtcc tactatcttt gaaagatatc agcgaagcga aaaagctttg atcgctgcca
     6181 tgattgaaat ggtcatttct ggtgtctcca ctcgcaaagt aactaaaaca gttgaactgc
     6241 taacggatgg tgcgactgtt tctaaatcat tcgtttcaaa tctgatgaaa cagttggatc
     6301 catttgtttt tgagtggaga aaccggagcc tagaaggctc ggaatatcca tttttcatgt
     6361 gtgatgccct gtatatgaaa gtaagggaaa atcatcgtat tgtctcaaaa ggtgtatata
     6421 ttggtatcgg cattgattct gatggacgac gtacgattct tggttttgat gttcaagacg
     6481 gcgaatcgga agataattgg gataccgttt ttcaatcgtt cgttcaacga ggtttgttcg
     6541 gtgtaaaact ggtcatttct gatgcacaca aagggttagt taaggctgtt cgcaagaact
     6601 ttttaggtgc gagctggcaa agatgccaag cccacttttt gagaaatatt tttgataagc
     6661 tgccaaagaa agtttcttct gatgtaaaag acgagttgaa gagtatcttt aaagcctctg
     6721 aacttgaatt gacacgtgaa agaaaagagc acttcttgga aaaatatggt tgtgattcaa
     6781 aattaagtgc tgcttgcgac attttagaaa acggctttga agatgctatt caaattttgt
     6841 cttttcctga aaatatccgc cgaaggattc gtacaaccaa tgtgttggaa cggttgaacg
     6901 aagagattcg ccggagagaa cgagtgattc ggatttttcc aaatatcaat tccatcactc
     6961 ggataattgg aacacttttg atggaaaaag acactgagtg gctggcttct ccacgaaaat
     7021 atttagaatt taattcgaat aacatttagc accatgaacc tgtgcgagtc tcttctttct
     7081 gaggagagac atcaagagtc gctgcgctac tgctcctgac ctctctcctc agaaagaaag
     7141 gcatggcagt taggttcaca acgcaaaaca ttgtgctttt gatgaggttg aacttttaca
     7201 caagattgtg gacttgactt atactagctg tttttgtgtt aattaaagga taaaatacaa
     7261 tataaagaaa catctgtttc tttatttaga ataaaaaaat acagactctg ttcaaataaa
     7321 tgaacctagc ctgcattatg agattgacta tactttttaa ttcattgaga tgaataactc
     7381 gtttttataa atgcccaaaa gtaatcggtg ccgaagccat tcctcgacct aattcttttt
     7441 caaattttcc tattgttgat atctccatat aaaacggtga tactggttta gacaattctc
     7501 ccctgattgc taacggacgt aaactacgtt ccaaattatt tatgacttta ggataatact
     7561 gacctttttc taagcctgtc tttgcgtcta tcaaaagtat acgttccttt tctttgatct
     7621 ctggatttaa aacgagatca tataatttta gtaccatgtt ttctatttct tcacttttag
     7681 acatcacgat ttcctcccaa aaatatattt cttcccacta cattctataa acaaagtata
     7741 cgctacatct agattttttt aaatcattac tggattgtta aaacctttca atacactgtt
     7801 atggcaacga tttttatttt aaaaacattc taaattaaat cagtaaaaat ttttattcta
     7861 agatttttat tagaattacg cctaatttgt gtctctcagg gcaattctaa gaacgtttca
     7921 agagatagcg acactatcgt tatcgaaaaa ggcgaaaacc tatctcattt tctgctaatg
     7981 taagttggtt tagcttttgt taatatgact ttatgaaaat ggaggtaatt aaatatgatt
     8041 tttcctaaag ttaatgaagc aagcttgaat aaaggatcct tttttcatat ctatgaaaac
     8101 gaagattttg aactgtataa tatcctttca caaaaagagg tcatcgaaaa ccaaataatc
     8161 tgccatccaa ttatttacaa atctattttc aagtatgcat tttttgcttc caatgatttt
     8221 caaagaatgg agattaccga ctgtatattc gaaaactgtg atttttcagg ctgtatattg
     8281 acaaaaagca tgttgcatcg cgtgaaattt gtcaattgta aattaacagg tactaaattg
     8341 atggaaagtt atattgggaa cacgttattc gagaattgta aaatggacta tgtaaattta
     8401 tctggttcaa acataaaaga aagtaccttt gagcactctg tcttatcaag cgcagatttt
     8461 gtagactgtt ccctcactaa aataatgttt tctactaatg atttagaaaa tgctagcttt
     8521 tttgatactg atttaaaaaa tatcgattta agtagcaact cctttgaaat gattgaagta
     8581 cgctcggaac atctaaaaaa ttgtacgctt aatcagttgc aagcacttgg ttttgctaga
     8641 aaatttttac aaataaaagt agtataatca aaatgaatat ataagataaa tttttagcaa
     8701 tacaaatatc cataaaaaaa cattgcctat tttaactctt catgtatact gactctatct
     8761 tcaaaaactg aatagtagga gttattatca tgaataacat tttaggaaaa aaaatagaac
     8821 ttaaaaaagg gatattgggc actattgtcg ttgtactcgg tttgttaggg ttgctatttg
     8881 cgccatccat tattagcatt ttttctttaa ctatcactgg atttatagca tttacacttg
     8941 tttttggtaa aaaagatacg aaaaaaatgt tctcgaaacc tgtcgctcct gtcaaaaata
     9001 tagccaaata tttttttatc aatgtagtaa tcagtgccgc agtctcggta tttttgcaac
     9061 aaattttgaa atggggttta acaggcaatc caataaatga ggctttcagc ccgttattat
     9121 ttattatttt gcctatcatg atacttggag aagaattgtt ctcagtgtat tttctcgcta
     9181 ttttttctag taagttcagc attcctgtcg ctagtttctt gtcagcaatc atctttggat
     9241 ttatccatta cagtacttat gataatggaa atattttaca cactgtagca cacatccttt
     9301 tgatacaagg ggtggcaaga ttgttattca accaagctgc cattaaaagc aactcgatca
     9361 ttacaagttg ggcagttcat gtgatatttg atttcacagc tatattgcta gtcgttttat
     9421 tctcctaaat ttttatagaa ataatacatg ctctattgct aagaaagcat actttatttc
     9481 ctttaaacaa aaagacagat gtatcaatgg attaaaaatc cttgatatat ctgtcttctc
     9541 ttttaatgcc agctataggg atcgaaccta cgacctacgc gttacgagtg cgttgctcta
     9601 ccaactgagc taagctggcc ttatccaaaa gaaattatat actctatttg gctgattgta
     9661 aagagtccga tggaaaatta gtttaatttc atgatatttt tcataaaacg tggtataatt
     9721 gatgtgaaag gcaggtttta tgaaaatggc aacttttgga aaatttgata caactatcgt
     9781 accatcggaa attggtgaaa aattcaagat caatactcac gtaatgttca caattcacgg
     9841 caagtcgcaa actggaacga tttcaaagca attgaaaaat tccgctgttg ttgagattga
     9901 tgaaactgca gaaaatgcga acttgatgac gcaaagtaag ggtgtagtaa tcgttaacta
     9961 caaagaacta aaaaagattt gaatttgacc atctctgcat ttttgggatg gtttttttat
    10021 atgcaaacga aagctgtgaa aaagctgttc tgcatcaaac gttaaaaatg accaaaaaag
    10081 gatgtgacaa aagttgtgtc acaccctttt tcgtatttac ttattttgta ccaaacaaac
    10141 ggtcacccgc atcccctagt ccaggaacga tataaccatg ttcattcaat ttttcatcta
    10201 gagaagctgt atagatgtca atatcaggat gcgcttcttg caaagctttt actccctcag
    10261 gtgctgctac caaacaaaca aatttaatgt tcgacgcgcc acgttctttc aatgcatcga
    10321 ttgccatgat tgctgatccg cctgttgcca acattggatc tactacaaac aactgacgtg
    10381 catcaatgtc ttctggaagt ttgacgaaat attcatgtgg ttccaatgtt tcttcatctc
    10441 gataaagacc gatatgtccc actttagcag ctggaatcaa ttccaagata ccgtcaacca
    10501 ttccaatacc tgcgcgcaag attggaataa tcgctacttt tttaccagac aacgtttttt
    10561 gtgttgtttc agtgatcggt gtttcgatca cgatgtcttc caatggcata tcccgtgata
    10621 cttcatatgc catcaacata gcgatttcat cgactacttc acgaaaaacc tttgttccgc
    10681 agtttttgtc ccgaataata gttagtttgt gttgaatcaa tggatgatcg atcacttgaa
    10741 atttgcccat tttttaagct ctcctttgtt ctgtcgtttc tttttaattg taaaacaaaa
    10801 tcaagtgatt gactagtcaa actcgccatt tttcctaaga aaatccgttt ttgaaaagaa
    10861 gttttcctgc ggacaagcaa agcttattct tttttcttga tccaagaagt agataatgcc
    10921 attgctgcac gcgttgcctt tgtctgatcc atagcattta ccatgacaaa atcatggatg
    10981 attccttgga agcgtacttg tgtaacagga acccctgcat cacgaagttt gcgcgcataa
    11041 gcctcacctt catctcttaa tacatccgct tctcccgtta aaatcattgc tgaaggaaga
    11101 tcatttagtt catttatatt tgctctcaaa ggcgaagctg tgatttcagc acgctcttct
    11161 tcattcgtcg tatattggtc ccaaaaccac atcattcctt ctcttgtcaa aaaatagttt
    11221 tctccgaatt catgatagga atctgtatca aaatcagcgt ttgtgactgg ataatacagc
    11281 aattgcttgg atattgggag gccctttcgt tcttttgtta agatcgtcat gactgtagcc
    11341 atgttaccac ctactgaatc tcctgcaacg attagattct cgagatccag attttttgac
    11401 ttggctaatt tattcagctg ttgcaacaca gcataatttt gttcaattgc tgttggatat
    11461 tttgcttctg gtgatcgact atattctgga aagacaacga cggaattcgt acgaaccgca
    11521 agttctctga tcaatttatc atgggtttga gcactgccaa aaacccagcc tgctccatga
    11581 atataaaaaa taactggtaa tttatccata ttcccttctg gtcgtacgag acgaatattg
    11641 agttttcccc attcgccagt atccaccatc aagtcttcaa tatcgacgga atatttgttc
    11701 acaggtgatt cttgtacttc ttctagtaaa actcttcctt cttccggtgc tagttcatag
    11761 atacgcggat gaggcgcatt tgcatcactg aatgctactg cttccttttc taacgagata
    11821 cgatcagtca tgacttttcc tccctgagat ttagtgtgaa gactaaaatt acacctcata
    11881 tactagagag catcaccgat attgtcaaag aatccgtttt cccaaaaaat aaacagaaaa
    11941 gcccaacatt gaacttttct gtttactaat ggaatttaaa tttttatttt ttatgcaacg
    12001 gaaatttttc agttaattcg cgtacacctg ttttgacttc gtctagttga gcgttatcat
    12061 cttttgcttg taatgctttg acaactagtt cagccacttt tacagcatct tcttctttga
    12121 acccgcgagt agtgattgct ggcgtgccga ttcgaatacc gcttgttttg aatggactta
    12181 gtgtttcaaa aggaatcgag tttttattta cagtgatatt cacactgtcc aaaatgctct
    12241 ctgcttcttt tccatttata cctaactcgc gaacatcgat caacatcaga tgattatctg
    12301 tcgcccctga aatgactctg gttccaattg cttgattgaa aactttgacc attgcttttg
    12361 cattggcgat gatttgttca ctatattctt taaatgcagg atctaatgct tctttgaatg
    12421 ctactgcttt acccgcaatc acatgctcta gagggccgcc ttgaatacca ggaaataccg
    12481 cactatttat tttttttgct aatgcttcat cgtttgtcaa aatcatgccg cctcgagggc
    12541 cacgcaatgt tttatgagtg gtagtcgttg tgatatctgc ataaggaact ggattaggat
    12601 gaagtcctgc tgctactaat cctgcgatat gtgccatatc taccatcagt tttgcgccta
    12661 cttcatctgc gatttctctg aatttcgcaa agtcgatcgt tcgtccgtaa gcactggcac
    12721 ctgctacgat caattttggt tgatgttttc tagctaaaat acgtaccaca ttataatcaa
    12781 taacttctgt tgtcggatca acaccgtaag caacaaagtg ataggttttg ccactgaagt
    12841 tgactggtga tccatgtgtt agatgtccgc ctgcggaaag atccattcct aagatcgtgt
    12901 cccctggttc taccaaagcg agataagcag ccgtgttggc ttgagagccg gagtgaggct
    12961 ggacgttcgc aaatttcgct ccgaataatt cttttgcacg gtcaatcgct aaattttcaa
    13021 cgatatcgac aaattcacac ccaccataat agcgatgccc tggataacct tctgcgtatt
    13081 tatttgtcaa gatacttcct tgtgcagcca tcactgcttc ggagacaaag ttttcagagg
    13141 caatcagctc caaattatgt tcttgtcttt cttcttcttt ggcgatcgca gcccaaagat
    13201 cagggtcaaa cgttttgtaa tctaccacaa acaacagctc ctttttattt tcctacaatt
    13261 ttatttttcc catcaaaaac aaattgccaa atattcgtat ctcttctatt acgattagtt
    13321 acgcttagtt tttcgttcac tgatagaaga tttacttgat agcattgtat catactaaaa
    13381 gaatgcgaca ataaccaatt gatcattttt caaaataatt ttgtcctgct gcttttttca
    13441 agcgattcat gtaagcattc cccaatccag tttctggata cacttgtaca aaaatcaaat
    13501 ctaatccaag tgttggttca tctaatcctc ttagacctgc aaacaatcct ttagccgccg
    13561 cttctactga gttgtcgtta tacatataaa cagctgcagt atctgtccgc acttgatccg
    13621 caatttcggg gcttgctatg acacctgcac ggatcttctt attttttgcc cattgaactg
    13681 cagttgacca atcgccatct ctgaccatca gaacacgtgt atcgggtgag tagtgtttgt
    13741 atttcatccc aggggctttt ggtgtttcat tttcttttac aagatgctga tcaatagcaa
    13801 caggagattc gatgactgct tcgatttgtt ctttcgttac tgcacctggt cgtaaaatca
    13861 taggcatggc tgttggatca cttaaatcaa gaacagtgga ttctacacca attcgcgtag
    13921 ctccatcatc aataatcccg gtaatttttc cttgtagatc atgatataca tgatctgcag
    13981 tcgttggact tggttttccc gacgtatttg cacttggacc gacaagcgga acaccggaaa
    14041 gttcgattac ttctagtgtt tttttgttat ccggtatacg aaatgcagct gtggataatc
    14101 ctcccgtcac aactgagggc aaagtgtctt ttttgatctt aaatatcaaa gttaatggtc
    14161 caggccagaa gttcttcaca atcttctctg ctgccggatg aaagttgtcc acatactctt
    14221 ttacttgttc aaaagaggcg acatggacga ttaatggatt atcttgcggt ctccctttta
    14281 cagaaaacac tttcttgaca gcgtttggta aaagagcgtt ggcgcccaat ccgtatactg
    14341 tttcggtagg aaaagcaaca agttctcctt ctttcagtgc ttcggctgcc tcttgcaatt
    14401 ctgtttcatc atatcgtttc gtttccacag tttatccaca accccttttt catacattct
    14461 tattttgtct gagtttatcc tcaagttttc cactgtttgt ggataatcta tttgtcaata
    14521 gtggataaac ttgttgataa accaaaaagt cgtttacatc cttgtcaaat aagtgttttt
    14581 ttataataaa aaaacaaata aaaaattttt catccacaac ttcaaccata aacttggatc
    14641 attcgttcat tgccaaaaag atcctttttt atcgccactt ttttatctgg aaatgcttgc
    14701 tgaaatattt tttttactgc ttcaccttgg cgaaatccga tttctaaaaa aatctttcca
    14761 tttgatttta gtacgcttgg tgcttcaact gctatttttt cataaatagc taatccatcg
    14821 ttctcagcaa aaagggccat tttaggttcg aatgaccgga cactttcatc catcagttcc
    14881 cattcattcc gactgatgta cggcggatta gaaataatca catcaaattg ctgatccatt
    14941 actggtccta atgtatctcc atgataaaaa ctgattttcg ttccttcttt ctgagcattt
    15001 tctttcgcta cttctaatgc ttcttctgaa agatccacag cagatatatg ccaatttttt
    15061 cttgctgctt tcaaactaat agcaattgct ccagtccctg tcccaatatc tatcacttct
    15121 aacggtacac caggcgtctc gtctaaacac cattcgacta actcctctgt ttctggtcga
    15181 gggatcagtg ttgcttcagt tacctttaac cgatgatcat aaaaatctgc atatcctagc
    15241 aaatactgag gtggatgatc tgcaagcaga agtcgcagat cggtttcgat caaccgctgt
    15301 tcttcttctg aaatctcttc gttcatatga aggagccaat caagtttttt ccattgtttt
    15361 cgttccaaaa ataaaaattg gatactatat gcttctttcc catgatcctc taaaaaagaa
    15421 gaagcccgtg acaggacttc tcgataggtc attttaccca ttttgcatag cttccagttt
    15481 tgatgtttga tcatacatga tcaatgcatc aacgatctca tctaatttcc ctgctaaaat
    15541 ctgatctaat ttttggatag ttaatccgat acgatgatca gtcacacgat tttgcgggaa
    15601 attatacgtc cggatccgtt ctgaacgatc cccagttccg actgctgatt ttcgattggc
    15661 atcatactcg ctttgtgctt cttgctggat cttatcgtac acacgagcac gcaagatttt
    15721 cattgctttt tcacggttct tgatttgaga acgttcatct tgcatagcaa caacgatccc
    15781 tgttggtaag tgagtcaaac gtacagcaga tgctgtttta ttgacgtgct gtccgccagc
    15841 acctgaagca tgatagatat ctgtacggat atctttgtct tcaatatcga tctctacttc
    15901 ttcagcttct ggcatcacga caactgttgc ggtggaagta tggatacgcc cttgcgattc
    15961 agttgaagga acacgctgga cgcggtgcgc accactttca tattttaatt tcgaatatac
    16021 gttgtctccg gtgatcatca tgatcacttc tttatatcca ccgattccgg taacgttagc
    16081 ttccatcact tctgttttcc agccttgtgc ttcagcatac ttttggtaca tattgaaaag
    16141 atcgccagcg aataatgctg cttcgtctcc gcctgctgct ccacggattt ccatgatgat
    16201 atttttatcg tcatttggat cttttggtaa aagcaagatc ttgatttttt cttctaagac
    16261 ttctttttct ttttttaatt cagataattc ttcttttgct agttcagcca tttcatcatc
    16321 cagtttttca ccaagcagtt cttctgtatc ggtgattcct tgtactactt ctttataacg
    16381 acgataaact tcgactgttt cgcgcgtgtt tgcttcttct tttgaaagct gcataaaacg
    16441 ctttgtatca gaaatcactt ctgggtcact taaaagttct ccaagctctt cataacggtc
    16501 ttcaattgct tgtaattgat cgtacatgct tattctcctt ctgtaatcat tgatgggtga
    16561 aagtaatgtt ttcgacaaac ggggtaataa gcttcatttc cgccaatttg cacttgatcg
    16621 ccttcataaa cagggtttcc atcaatataa tgtaaattca tgattgcttt tttgtggcag
    16681 aaccaacaga ttgttttcat ttcttcgatt ttgtctgcat aaagcaataa atattttgat
    16741 ccttcaaata actcgtttcg aaaatcattt ttcaaaccaa atgccataac cggaatatcc
    16801 aattcgtcca caattctcgt aaaagcaatg acatgtttct tttttaagaa ttgcgcttca
    16861 tcgatcagca cacaataggg tttgctttcc attttctgaa ttacgtcaaa tacgtctgtt
    16921 tcttcaaata tcggcatcgc attacgtttc agtccgattc ggcttgaaac aacgcctaaa
    16981 ccatctcttg tatcaatacc gcttgtcatt aagacaactg gcttattttg ttcttcataa
    17041 ttatgtgcga cttttaggat ctcgatcgtc ttgccgctat tcattgcgcc atacttaaaa
    17101 aatagttgtg ccatgctctt tttcaccttc cagtttgttc ctatggtatt atacttgatt
    17161 ttcagcattt tttacagtga gaattttcat caagcacaga ttagtattaa atctattgct
    17221 cacttttgtt ccaatttttt gacaagaact aaacatgggt tccattcatc ccataaattt
    17281 ggaaaaactt ccaaccttga aaatcctaat gattcgtaga aacaaatagt ttgatcatat
    17341 atagagtagt gcccctcgtc tacagtcttc acttgcaaat atttgtaatg ttttgctgcg
    17401 taaccttctg cttctttaaa aagttttgtt cctaatcctt ggtgatgata gtcttttttt
    17461 attcccatac aatagaatag atttcggcag tatcttcact tgtctgtgaa agagtgataa
    17521 agcctaaata ttcttctttt cttttttcac accataaagg tagtttactt gattcctcga
    17581 tatatttttc cgttgattca ggaagcccaa accactcagg caagtctagt aatatttctc
    17641 ggacacactt tcctttttcc tctctatctt ttacaaacga tatcggcata ttctttctag
    17701 attctccctt tttgaattga gtatagcatg ctttctttct tttcacgtgc tataataagt
    17761 taaaatttca aagttttacg taaggagcta tttgatgaca aaaaatattt ttgattccat
    17821 cgctaaccat tatgattccc ctgaccgtca agcattagcc aagattattc gcgaagaact
    17881 aactacttat ctaccgcgcg acagccacca aaaagtcctg ctggattacg gaggcggaac
    17941 tgggcttgtt agtttaccgc ttgcggaaag attcaaagaa ctgatcattg ccgatgcttc
    18001 agaaactatg ctgaaaatgg cagaagaaaa gatccaagca gcagatctga aaaatgtccg
    18061 aacgatccac gctgatgctt ctgtcgaatt tcctgctgtc caagctaatc tcatccttct
    18121 atcacttgta cttttacaca ttcctgatac ggagaatatt cttacaaaac tttatgaaat
    18181 tttagctcct ggcggtcaat tgatcatcgt agactttgac aagaatgaac aaattagtca
    18241 tccgaaagtc cataatggct ttagccaaga agaattgaat aatcgattgg aaaaaacggg
    18301 ctttgtctct gctactagtc ataccttcca tcgtggggaa aagctattta tgaacaaaaa
    18361 cgcttcgctt ttcttatcca tcagccaaaa agcttaaaat gagattgcaa aaaaagtgag
    18421 caactccaag aaataagaag gaattttcgg aaaaagaagc tgtgacaaaa aacatgtcac
    18481 agcttctttt tattttttca tcacatgatt ttaatttctg aaagaggcca ataacggaaa
    18541 acaacttttc cttggatcat atctttgtcg actaacccaa acattcggct gtcttttgaa
    18601 atcaagcggt tatcgcctag aacaagatac tttccttttg gcacagtata ttcaaagtca
    18661 ctcgtaaagg tggatgaact ttctgcttgt gcttggaatc ctgagctata agcatattct
    18721 ccttgtaact tatcttctga aaattctttt ttgaactcat ccagataagg ttcatcatac
    18781 tttttgccat taatggtcaa ctggtcattt tccatcttga ctgtatctcc aggcataccg
    18841 atgatccgtt taacaatcat ccgttcttca tcccccggtt cctttgtagt aataatatcc
    18901 attcgttcat agtttgagat cttagatgaa atcaatcgtt gtccatcact taatgtaggg
    18961 tccatcgaat gcccgtttac agtaactggt gtaaacacgt acactctagc taataaaata
    19021 gccgctaata caaggatcaa aatcccccaa tttttcaaaa attctttcac agcttacctc
    19081 ctacttgtat tttcctcgtt atttattacg ttttttcaca atacttcgtt gtactttctt
    19141 tattctacaa tgtttataaa tgaaaggaaa gaataagtga tgtgttcgtc ttatctttct
    19201 cgttatataa aaaaggaacg tgacaaaaga cgtgtcacat cccccttatt ccgaataaac
    19261 gtttttatcg caaccactga tacacggcgt ttcatcacat tatgaatgtc ttcttctttg
    19321 gctacgacta taaaccactg gttcttcctg cgactgtgcc ttttttgcaa tcaatttcag
    19381 acgataaggc tcatgatagc gtaacccgtc tttgacaaaa tcaccgccga cgatgaacaa
    19441 tccgacaggt tccaatgtgt cttctgttga gtcaagtcga atggtcccta ctttttccat
    19501 gtccgagtag cttcgtccaa tatttagaac atactctttc tctcctgttt tcttgacata
    19561 gtaataagga tacaatgtat ttctatcgac cagaaatcca tccatgcgac cgagttcatt
    19621 tgttttgatc tcaccatttc caatcaaata ccgcatattc tcggctaatt tcaattgata
    19681 atcttcgtca taagaaacca tgacctcttg tgtattcact ttatcgatcg aatttccatt
    19741 atctatcgat tcttctgtct ggatgttgaa cgcttcatca ggtaaatact tcacaaaaag
    19801 ctgagtgaca tcggctttcg tgtttttatc taaaaagtaa taaaatacat ttaaaacagt
    19861 aaaataaaca agcactagaa atgcaagtcc atataagaaa aattgaagat agttttggtt
    19921 ttgccaaagt ccataaacaa ttctcaataa ataaagcgtg ggaatcacac ttaaaatcgt
    19981 atatccacgg ttaagatatt taggactgat gtctaaatag ccaaggaatt tatgcacccc
    20041 gttgataagg tctagtaaaa atgtcattga gaatcacctg cttgctggtt atacgtttgg
    20101 gtatttcctg aatcttggtt ttgctgtgct gtacgtgctg catcatcatt ttgcgtgtta
    20161 tcatctacag tcgtactgct tggtatcgtt tcggagctag cacttgtatc cggacttgta
    20221 cttggattgg ttgaatccga tggagtgcca tctggtaccg tattacttgt ttcgcttgag
    20281 ctgtcagttg tatatgtctc atcatacgac gaactagttg ttgtatcgtc ccatgtactt
    20341 gtcgtagtgg tttcattcaa actttccgtt gttgttggtt cagatgtcga gactgaagta
    20401 gttggtgttg tttcttctac agtactagaa cttgaacttg gtgcacggta gctggaacta
    20461 ttcacaatac ctgtagtagt cgcgacaatc gtcgagattg ttaatacagc gattggcttc
    20521 aaaataggtg ctacttttcg catcttatct cttccttcat tgaaatttat tcttgtttcc
    20581 ttactttact acggacaaat tatacagtct accttatttc taccataaat tctttatttt
    20641 tttcttacgt ttttccgaaa gatttcttta attttcctca aaaactacgg ttgatgtttt
    20701 taagtaagct tttgcttccc gattgatttc tttcagatcg catccttgct gcttggcagc
    20761 gaacacgcaa ccacaataac attggcgata aatatcatac tctttgcaca tttgcaaaga
    20821 gcgttcatag cctttgtttt tcttgaaatc acttggcaag tattgtgtcc cgtaaatctt
    20881 ttggatatcc attccgatct gattgatcaa agctgcattt ttctttggcg agatggtcag
    20941 ggcacttcca aagtaatcgt atcctagttc ttgcgctttt tgcgcaacaa gatctaatcg
    21001 catattgaag catgcactgc agcgagcacc accttctttt tcgttttcaa gatggtttgt
    21061 taaaaccatt ttggtgaatt catttggacg gtattcatca acgattagcg caacatgatg
    21121 gtcagttttc tcattaaatt ggtctacaaa tcgtttttgt tcatgtaatc ttcgcaaata
    21181 ctcactttta ggatgaatat tcgaatttga aaaaaataac gtgatctctg catattgaga
    21241 catgaattcc aatgtgtgcg tactgcaagg agcacagcat gtatgtatta atatcttagg
    21301 ctttacttct tctttctccc attgcatgat catttttttc agaactgtat cataatttat
    21361 tttttgattt ttcatttttt ctacgatttg ttgagcatta atcaatttgt tattcctcct
    21421 ccttcttttt tctaggattt tccttattat aagccatttc tattttaatc gaaagctgta
    21481 ttgttatctg tttgataatt tctttgagta ttcctaattc cccttgtata ctggtagaaa
    21541 cgactattgt caggaggata gtactgtgca tgttgattta ttctttcgaa ggctgcagcg
    21601 gcgaggacag aagtttgctg ccaatcattt ctcatcgatc caaatcatcg ttttttatta
    21661 tatattgatg acggtcttat cactcgttct attttatatg ccgatttttc gcgaaccaga
    21721 tagccatgtt tcttttgtgg acatgctgtt catggcaatc agtacagtga gtgtgactgg
    21781 actatcgact tttgatatca attctgtgtt caatgataac ggtattatac tgctggaaat
    21841 attatttcaa gttggtggtt tggggatcat gatgatttct actgcttttg tcattttttc
    21901 taaacgacgg atcacactga aacagcgaca gctgatcatg acggatatga atcaaccgcg
    21961 tttgtctggt atcgtccgac tgattcggat cacttttgct attttaatct ggtttcaatt
    22021 actttttggg acattctttt ctatttattt ttattacaga ggctattttg atcgttggcg
    22081 tgatgccgtt ttttacggtt tttatcaggc gatatcggct gtcacgaact ctgggtttga
    22141 cgtcactggg gattccatca agccctttgc acatgactat tttttcttga tcctgatcat
    22201 gtttttgatt tttgtcggag gaattggttt tcctgtattg atggagtgcc gggaatggct
    22261 attatacaaa cgttccaatg cgaagcttcc gtttcgtttt tccttgttta ccaaattagc
    22321 tgtgttagct tttgttatct tgtttgtttc gggaactgtc ttgatttatc tattggaaaa
    22381 ggaccattta ttccaagatt ccaatatgag tgtcaaatgg atcaattcga tgttttattc
    22441 gattacgact cgtaatgctg gtctgcagat tcacgatttg ggtgattttc aaatcacaac
    22501 actgatcatt ttttctttac tgatgtttat cggatgcagt cctagttctg taggcggcgg
    22561 gattcgaaca actactgtag caatcatcgg tttgtatcta tattccttcc tcaaaagtga
    22621 agataacatc aatatttttg gacggagaat cgatcaagat gatgtgcgaa aatcagtcgt
    22681 tgtctttatg ctttctcttg gtatgtgttt cttttgtatc gtttttttat ctgctacaga
    22741 agaacaaacg ctgatctcga tcatcatcga agtaacttct gcgtttggaa caactggttt
    22801 atctttaggg attaccggag acttgtctgt tgtgggcaaa atcacgattg ctacactgat
    22861 gtttattggg cggatcggaa tgctatatac attgatgtta ttcgtgccaa aagaaacacg
    22921 agatctaggc tatgaatatc caagcgagaa aattattatc ggttaaaatg ggaatatagg
    22981 taagagggaa taataaaatc acagcagctt gaaacaatca ttttgtttca agctgttttt
    23041 ttgttaaatc atgcgatttt gtccgatttc atgcactttc agccgtaacc ttttcttaag
    23101 aataagaaaa gcaatttaac ctttttttca acatatggta tgattagttg aatttattcg
    23161 tcagcaaatc gtttttggag gttacataat gggaatacgc agtcattttg cgattgcagt
    23221 tggaaaaacg tcgcaatggg cattaaaaac tttttttaaa ggcggatcaa gtttaccagg
    23281 taaactagcg ctgaaagtcg atccacatat actagatagt ttggcaaaag actaccaagt
    23341 cgtcgttgtc accggaacaa acggtaaaac attgactacc gctttaacag tcaatatttt
    23401 gcgccagcaa ttcgatgaag ttctaaccaa ccctactggt gcgaatatgg tacagggaat
    23461 tgtatcgact ttcttgcagg caaaaccaaa aaaaggacag aaaaagtttg ctgtcctaga
    23521 aatagatgaa gcaagtttga gcaaagtcac agaatatatg aaaccagaac tgttcttatt
    23581 tacaaatatt ttccgtgatc aaatggatcg gtatggagaa atctatacga cctataaaat
    23641 gattgtagac ggtgccgcaa agtcaccaaa tgcaacgatt atcagtaacg gtgattcacc
    23701 aatcttcaat tctatcgaaa cagtcaatcc tagaaaatat tatggctttg accatgaaga
    23761 agatcgtgaa caaatggctc attacaatac agacggtgtc ttatgtccaa attgtcatca
    23821 catcttgcat tataaaatga tcacttatgc aaacttaggg aaatattact gtccaaattg
    23881 tgacttcaaa cgtcctgaat tggattatcg attaactgat atggtacgga tggacaatgt
    23941 gtcagcagat ttcgtgatcg atggtcatga atacggaatc gaagttggcg gtatgtataa
    24001 tgtctacaat gctttagcag ctactgcagt ggcagagtac ttcaatgtcg atcctgataa
    24061 aatcaaacaa ggtttaggat atgacgagaa agtattcggc cgacaagaag tcatcaatat
    24121 caatgggaaa aaatgtacat tagtacttgt caaaaatcct gtaggtatca atcaagtaat
    24181 cgatatgatt ggtctagctc cttatccatt ttctttagtg gctttattaa atgccaatta
    24241 cgcagacgga atcgatgtca gctggatttg ggatggtaat tttgaaaact ttgccgatat
    24301 ggatatccca gctgttattt ctggaggaga ccgtcataca gatatgactt tacgtctaaa
    24361 agttgccggg attccagaaa ataaattgac agaaacaaaa gaattaccag aagtaatcga
    24421 aaaaatcaaa gaattgccaa ctgagcatgt ctatatttta gctacttaca ctgctgtatt
    24481 gcagcttcgt aaagaactag ctgctcaagg atttatcaaa ggaggaatga accgtggcta
    24541 attatgaact caatatcgcc cacctttatg gaaatttgat gaacacatat ggtgataacg
    24601 gaaacctttt gatgttgaaa tatatagcag aaaaaatggg ggtttcttgc catacggaga
    24661 tcgttagtat acatgaacct tttgatgctg ataaatatga cttggttttc tttggcggtg
    24721 gtcaagactt tgagcaatta atcatttcaa aagacatcca gaaaaagaaa gattctttga
    24781 tcaactatat cgaaaatgat ggcgtcatgt tagccatttg tggtggctac caattgctag
    24841 gtcactacta catgggagct aatggagaaa agatccaagg aatcagtgca ctggatcact
    24901 acacgttaag tcaggaaaat aaccggttta tcggtgatat cgttatccat aacgaagaat
    24961 tcaatgaaac ttattatgga tttgaaaacc acaatggtcg gactttctta ggtgaaggtg
    25021 aacgaccttt ggggaaagtg gtacaaggtc aaggaaataa tggtgaagac caatctgaag
    25081 gtgtcattta ccgaaatgtc ttcggttctt atttccatgg acctatcctt gcaagaaatg
    25141 agaacctggc agctcgtttg atccgcctag ctttagaaca acgttacggt aaagaattgg
    25201 atctgcccta tcttgaagga caaacagcct agttattcga aaaaggggaa tgtgacaaaa
    25261 atttcgtcac attccccttt ttcgtatcct attttaagcg acttgcttgg cataacggtt
    25321 cacgttttgc ttgatattcc cgattgtttt cggatcattg attcctgtga cttcttgaat
    25381 caattgttcc aagttttcat ggatcttcat ttcatgcaat tgacggcttt gttcatcttc
    25441 aggatcatag taattaaaaa taatccccat tgttgccgtc aaatgtgggc aagtcaagtt
    25501 atgttcttgt agttctctca ctggacgtgt aaatctttct tgataaccta acttacgtaa
    25561 tggtgtccga gcgacacgtg tgatcgcatc agagatattt ttgttttgga agcgatgaat
    25621 gattttttca atatacgcat gatgctgctc tgcatcaaac ccccatttgg caatcagcaa
    25681 acttcctgtt tcctgcaaaa ctgattttaa ttgtgccaca actaacgcat cttgcattgc
    25741 ttcatcaatg gtctggtaac ctaacaaggc acctgtataa gcgacagtcg catgaccagt
    25801 attgacacta aatagtttgc gttcgatata tggttctaga tcatctacat ataaaacccc
    25861 ttttaattgg atttttttat tttttctttg gctatcgtca atgacccatt ctttgaaagg
    25921 ttccacttgg acgaataaag gatcttcatg gtgttgtaat gggacgattc gatcgactgc
    25981 tgcatttgga aagccgatat atttgtcagt aaatgatgtt tccggaagat atttatacac
    26041 ttctttttct agaaatgctg aaccgccaat catattttca caggcaatga tatctaaaga
    26101 tgttgtgctt ccttcctttt ctctttcttg gatacctttt gcaatcaact cagcaataaa
    26161 aggtaagata ttcgggccaa tggcggtagt aactaaatct gcttcagcga tttcttggat
    26221 tactttttct ggatttgttt gattgttgat tcctttgaca ttttttacag tgattttttc
    26281 ttttgattca tctgctaatt cgatttcgta ttcttttctt tcatttaaag catcaatgat
    26341 cgtgtgattc acatctacga aagtaatctc aaatccatta gctgccaaaa cctctccaat
    26401 aaatcctcga ccgatatttc ctgcaccaaa atgtactgct ctcatttttc tctctcctta
    26461 agcaattgct agattttccg ttacttcttc tttgctgagc gcgtctgcta gctggacaac
    26521 attgtccata tcactgcaat aaagtgcgat ttgctgtaca agttgcaaat gctcatctcc
    26581 gactcctgca ataccgaaca aaacagtcgc aatcttttct tcttcttcgg taccaaaatc
    26641 tacaccatct ggtacttgaa cgacacagat gccggatttt ttcacatatt tttttgcttc
    26701 atctgttcca tgaggaatcg cgataaaatt ccccatataa acagataaca ttttgtctct
    26761 atctaccatg gcatcgatat attcctcatc aacgcaccct gcttctacaa gcatctgacc
    26821 gcaatagcgg atcgcttctt ctttggtttt aaaagatcga ttgagctcga tcatgtcgaa
    26881 tggcaaactt tgcatgctgt tttcctccct taagctgtct gtttcaaata ttgtgcaatc
    26941 gattcgtatt tttgcggatt gaccatgctg ttgactgata aatgaatgga ttgaggtgca
    27001 gccatctgta ctttttccgc taaactttct ttggctacga tcagtgaatc aggttcatct
    27061 tttaaatctt ggatattgac ttttcttaac gggatagtta aatggttctt gtctaatata
    27121 tctttgagca cggtcatccc cattgttgct gatccggaac gatcatcgtg gacaagtaga
    27181 atctctttga tttgtttgat gtttgctgaa agaaactctt ctgctggctc attctcaatt
    27241 tcagaaactg atgttttctg cttgctgctt aattcttgaa tgatttcatc atatcgaggt
    27301 gaattcaaaa agttttctac tgagacaaaa cgcgcgctcg gtgctttttg ttttgctctt
    27361 tcttgtagtt cgacctgagt aacgatcaat gtattcgagt catctgtcag ttggctgatt
    27421 gcttgattgg tcaccgtttg aggcaagtct gcttcctgta ctttttttct caagatcgaa
    27481 gcgcccatcg cacttgatcc cattcctgca tcacaagcaa aaatgatttt ttgtacgcca
    27541 gagaatgtag ctccttgagg aataccttgg gcacctttgg aagcttgctt agctgccttt
    27601 gtctcttgaa cagatttttc gaagtcttca tttgtatctt tcgcatccgc tttcaaaatt
    27661 actgcagaaa taccaaacga aacagctgcg gcaacaatca cgcccaaaat aacaggaagg
    27721 taagaattga acggtgtcat cgctagaatc gcaatgatcg accctggaga agctggcgct
    27781 cttagtccag catccatcaa ttggaaaatg aaacttcctg acattcctcc gcctataact
    27841 gataagaata ataacggttt catcataacg taaggaaaat agatttcatg gatcccgcct
    27901 aaaaagtgaa tgatcatggc tcctggtgct gaagattttg ctgctccttt gccaaacaaa
    27961 gtaaaagcaa gtaatacgcc aagtcctgga ccaggatttg cttcaagcaa gaatagaata
    28021 gattttccag cctctgctac ttgttcagtt cccaatggtg tcaaaatccc atgattgatt
    28081 gcattattca aaaataagat ttttgctggt tcaataaaaa tacttgtcaa tggaatcaaa
    28141 tgggcagata aaatgatctc tacacctttt gccattgctt gagtcaatgc atcgacaact
    28201 ggaccaatcg cagaaaaacc taataacgct aatccaaaac cgataagacc tgctgaaaaa
    28261 ttattgacta gcatttcaaa accggatttg atccgttttt ggaatagctg atcaaatttc
    28321 ttgatggcat atccaccaag aggtcccatg atcatcgcgc ctagcatcat tggaatatct
    28381 gttccgacaa tcacacccat cgttgcaatt gccccaacaa ctgatccgcg gtcaccagca
    28441 attacttttc cgccagtata accgatcaat aaaggaatca gataagttag cattgggctt
    28501 accatggtag cgaaagcctc atttggtaaa taaccatctg ggataaatag agccgttaat
    28561 acaccccagg caatcagtgc tccaatattt ggcatgacca tgcttgataa agtactgcct
    28621 aatttttgta ccctcgcttt tagcgaaatg ttgtttttgg gaatcgtccg ttccattttg
    28681 tctctccctc tctccttttg atactctaat tgtatccgct tactaaaaag agaaatagtc
    28741 gtactttggc acgaaacttt gtgccaaagg tttgagtatg tcaacaaaat aaaaagattt
    28801 cggtatcaga aaacttcaat tttaaataaa actgttattt taattttttt ataatatgca
    28861 aacaataact tttgttagca tttaatttag atgttttcca ttctattatg ttattaaatt
    28921 ttatattgga ggcctatcta tgacattact gatcaaaaac atgaacatga aacgtggtaa
    28981 aaaacgtgtg attcgacaat tgaatctttt agtagaatca ggagaagtag ctgctttgat
    29041 tgccccgaac ggatttggca aaacaacttt gctgaatggc attgcccaac tgctgccaac
    29101 aacttatgat caattgtccc ttcacaacat ctctcctact gcttatacag attatcgccg
    29161 gtctttcttt tttctagaat caacgaatca gttatccgac tatctatcgg ctgttgatta
    29221 tttgactttg atcaaatact attggcagag caaagtagaa tgccaaactg ttttccaacg
    29281 attgaagatg actgagtacc ttactttgcc gattggaaaa atgtctctag gaatgaaaca
    29341 acatgtcttg cttgctgctt acttgatcag tgattcatct gtcatgttat tcgatgaacc
    29401 gttgaacgga ttagacccag gaagtattga tatcttgaat agcctcatca gacaatggca
    29461 gaaagagggg aagacgattc ttctttcttc ccacgctatc gcgaatatcc aatcgatttg
    29521 ttctaaagca tgttttctaa aaaatggaga aatcgtcaca gaatcctcaa atatggaaga
    29581 gattttagct atttatcgta aattatatga ggtgtaacta tgtctttact tacatttgaa
    29641 cgcaatctct tactaaaaaa caaagtaaac tttttatttc ctctattgtt ggtcgtgctc
    29701 tttgcattcc cacttttttt tgatcataaa cttgcttata cagaattcga tgaactgaat
    29761 cataactatg aagagatgca acgtttgatt gaaacgttga aagaagatga gaatgagaaa
    29821 gaatttgtgg aaagtttaga gaaaagcaac aaactgatcg aagcaatctt acatgcaaaa
    29881 aacacaggga atgtccagca aatggtcgaa gcaacttatc attatgaaaa agatatactg
    29941 gatcgactga tttctggtca aagacaagga attccaatca ttgaacaaca aaaacgtgtc
    30001 gagttgctcc gttatatgaa agaacatcag atccaaagat attctatttt tgacttgcca
    30061 gcgcatctct ctttagcaaa ttactacgaa aatatttttt caggaatgat ttctagtttt
    30121 ttgattttat gtataaccgc cttgtttctt tcttccatca tctcttatga aaaaagaaag
    30181 caggtaatct ctctagtcaa tctattaccg gatagtatgg taaagaaaca tagcatccgt
    30241 tttaccattt attatggtgc agctatgcta agtctcgtga tgccattttt gatcgtctct
    30301 atactagtaa taatcaaaaa tggtttggga gacttccgtt atcctgtagg aacgatcatt
    30361 ggtcaggaga tacgaatcct gccgatgtat gagtatcttt tccagagctt tctgttcctg
    30421 ttgttgtggg tgctcttttt aagtacaatc agttttcttc tatctgcttt atttgaacat
    30481 tctctagtca atcttttagg tacacttctt tgtcttttct tagcagagta tcgcttgttt
    30541 tcttcgattg gatggataga atcaatcagt cattacttgc caacttctta tgttgatttt
    30601 caaaatgtga tcataggagg agatattttc tcgccactag cttcagaaca ggttactttc
    30661 atgaatggta tcttgacact tggtatatgg agtatcgttt tattattcat aggtatgggc
    30721 actatctata taaaaaagag ctattaaaac acaaaaataa taggggcggt gacaaaaacc
    30781 ttgtcaccgt cccctatttc aaataaacgg ttttctagaa taaatagttt tcaaaagctt
    30841 gcatccgcag taataagaac ctcccaacaa aaatagctcc tcttattttt atcttggttg
    30901 aagttattac ttgatgcaag gcagcttttt ttactgtgaa cattggttat atatgtccat
    30961 gttcaaaaat catttggact tcttcaatgg tttccgcatc actgagcgcc attacttggt
    31021 cgatgtctga acagaaaaag gctaaatctt ggagcaccgc taactgatgt tcttttttta
    31081 aagccactac aaataagagt gtcgcgattt ttggttcctc ttttgttcca aagtccacac
    31141 catcaggcac ttggactaaa tagattcctt cttctactac ttcttcatct ttgtcagtcc
    31201 catgtggcaa ggctacgaaa ttcccgatat agacactcgc tttttcttgt ctttcgatca
    31261 ttgattggat atatgccggt ttgattcccc cttgttgggt catccactgg ccgatctcgg
    31321 tcaacgcttc tttccaattt tgataggatt gatcgagcaa tattttttct gctggtagct
    31381 gtatcatctt ttcaccttca tttctttatt tcttcaatca ggattttcgc taacaactga
    31441 taaacgattt ctttatttcc tgagcggaag atctcggtgt tcaagtcatt catgatgatt
    31501 gctccactga tctttcctaa aagaatcccg tctttttctt ggattggcga aggcgccagc
    31561 ataactaaca tacgggacaa atcgactggc tgtctatcca tcccttcaat ggtcaacgga
    31621 tgtttcaaat tgaaaataca aaagatcggt ttagaaatca atgagctgga tgtatggaat
    31681 aaccccatat ttgtatgagg aataccgata ggtgcttgtt catatcgctt aagcagccgg
    31741 cgatgaacca cctcttcatc cgttactagt ccttcaggaa aataaggaaa taagctgcga
    31801 atcgtttcgg aaatcgtctc aggattatcc agttcttgga taaaaaaacg accgagtaat
    31861 tgattgattt gatccatgaa atgcattgtt tcctcataag tatcttctat catccactcg
    31921 ttattgctgt tatacgtaat tttttgcctt tttttcttct caatatgcga aaaagcttct
    31981 tttagctgct tgacttcatc ttctagcagt agcggggaga ctaaaatata ttttccaccg
    32041 tatcctggta aaatagatgt agaaataaca acatcatatt cttcttgtaa atcgattttt
    32101 tttaaatcag caatccgata gaaatgaatt tgttggatga agggaaaata ccttttcaat
    32161 cgtagttcca acatgctagt agaagctaat ccacttggcg aaaatcctgc aatactgata
    32221 ttgctttctt tcggacttcg ttccaatgaa ttcgcaaaat gaaggaccat ataagcgatc
    32281 tcttcttcgg ataatttttt ttcttgaaaa atccgctcta ctgctttacg gattgcattc
    32341 gcaacttcct cgtattgaac catgattttt tctaaaatcg gattggtcag tgtctcttct
    32401 tgtaaaacgg ctcttgaaaa cacaccagat aaatgagtca acagcatttt gtacaaagtt
    32461 tcatcttcat aaaaatggac attcgtctct tcacttactt cttgaatcag ttgttttact
    32521 aaataagcaa gctgtgtatc gaacgtctct tcaaagaaat tctgatcaaa tgagttcgtg
    32581 aagtcattta ataatcctgc aaaaaagacg atctcattta ctggataaag ttgttttgtc
    32641 tcagaagcga tccgtgaaaa caatcgttta gctgtctgta gtaattcttt gtttgccaga
    32701 ctattatatg tctcatttgt cagtaaatga tttttcgtca cacgctccat cgtcaatgtc
    32761 agcatcaaaa ccaaatgttc aagctttcta tcacttagat tggtcttttt ttggatcaat
    32821 tcttccccga ttactttgcg tgcaagagat agatgttgcg gatcaacgaa tcgaaaaaaa
    32881 taattcagtg atgcatcgaa agaaatacta tgaaaaagtt catattcatt gatttctaat
    32941 tctaatacat tcgctgtcag aagccggcgg tatttttccg gtccttcgat ctcgtatccc
    33001 tggttccgaa tgatttccaa aggcagttgg cgaatacttt cttccaactg tttgatatct
    33061 gcataaaaag tggtattact gatgcgatat gattccatga aatgagcaag actcaacggc
    33121 tctgtcgtca gtaacaactc ggctaaaatc gcgtgttgtc gtttagcagt ggataagtca
    33181 ttacttttcc catctgtatt ttcttttaat tgctctaata cttcttctga tgcctcaatg
    33241 acaaatgagc ctctagctgc ttttttcaaa gaagcattta ccgatttcaa tgagacttca
    33301 agattatcta attcgcggta aatagtccgt ttactgacct tcaataaatt catcattttt
    33361 tggatagata caggtgacgg gctactgaga aattctgcta ataattgttt ttctctgggc
    33421 gataaataca tcttatattt ctcccttcac gagtttttcg tattcattcg tagctaccca
    33481 atgttcaact agaactagcc gagggatctt ttcttctagc tgttcagtta cagctagttc
    33541 tctttgtgcg atataaatcg tttcttcttg gattttgatt ttttctattg attcttttga
    33601 gataacaaac tgttttttcg ctttttttgc taattgactt aaaatcgtca tgcccatagt
    33661 ttgtgagccg cgtctgttgt ttttatacaa aaatacaata ttagcaggcg acgttttttg
    33721 ttctgtgata tctgctatct cttttttttc ttcccgcttt ggaaattgga tcgcttcttt
    33781 tattcgtgac agcaagatat ctgtttccaa aaagtgatcc agtacataga tttgactttg
    33841 aggtactttt tctcttgcaa gacccgctag ttcatgttgc acgataacca gctgatttac
    33901 ttggttattc aattcataga tagagctgta tccgacagaa agatcgattt tttctgctgc
    33961 taatttcttt ttcagcaggg cggctcccat agcacttgat cccatacctg agtcgcaagc
    34021 aaaaatgacg gaatgaatga tagatggttc caagatttct cccctttctt tgaccgtttt
    34081 gttctctttt tctttatttg gtaatttcaa tagtatcgct gcacaaatga aggtcacaaa
    34141 tgtactgatt gctatcccta tacttacccc agctagttgt ggataaggcg cattggcaaa
    34201 aatcacgagt agtgacccag gcgatacagg tgcagctaat cccgtatcta accattgaaa
    34261 tatcaacgtg ccgcttgccc caccagccat tacagctaaa aatagatacg gtcgcagcaa
    34321 gacaaatgga aaatagattt cgtgaatccc gccaatcgca tggatcatca atgctccact
    34381 cgcatttact ttttcctgtt tttctgcaaa acaaaggata gaaagtaaga tccctaaacc
    34441 aggtccagga tttgcctcca tcaaaaataa ggaagagtac ccgactgttt gtgagaattc
    34501 gattcctaaa ggtgtcaata caccatgatt gattgcatta ttgaaaaata gtacttttag
    34561 aggttctaag atcagactga caagtggtaa cagattcatt gtgactagcc aagacacaac
    34621 ggaggcgagc aacaagtgaa cactatttaa aactggttca ataaaaacga ttccaattat
    34681 acaaataacg ctaccagtca gtcctactag taggttgctg acaagcattt catatccagc
    34741 cttgaatttc acatggaatc tagaaacaaa ttgttcatat acgatgccgg caagcggccc
    34801 gataatcatc gctcctacga tttgtggagc attggaacga gcgatcaacc ctaaagaagc
    34861 aattgcaccg gcgactgccc ctttttgtcc ccctaccgat ttcccgcctg aataactgat
    34921 caagatgggt aaaagcagct ggatcatcca atggcatacc tcttctaatg tggtgtgcaa
    34981 atcgccagac acaccttttg ttgctaatga caagattccc caagcgataa acagacttag
    35041 atttggcatg atcatttcat ttaagtgatg tccagctttt ctcagttgtt tcatagacac
    35101 gcgactgctg ttgtttgctt tccatttgta ttccatcttt ttcaccagac ttctatttat
    35161 tgatggacgg attttatgta gaaaaagcag caaacgaaaa accgtctgct gctttcagct
    35221 atctgggaac tatttgcctg gttcagacgc aatgattgct agttcacctt ggaccgccca
    35281 ctcagaaacg ccatttggaa taatacagct tgttcctttt tctaatggat atgtttttcc
    35341 gtcaacgaca aggtctcctg ctccttcgat taccgtcatc aatgtataag gggcttgttt
    35401 tttgaaagat gtgattcctt taatatccca ctcatataca ttgaaaaact cagtttctaa
    35461 ataagtgacg atcgatgaat tcccttttcg gacttctttg atttgtaatt ctggtgtttt
    35521 cgcagggaca gttgttacat cgatcgattg ttggatatgc agctctcttg tttgtccttg
    35581 cgcatctttt ctgtcgtagt catacacgcg gtaagtcgtg tcagaacttt gctgtgtttc
    35641 taaaatcatg attcctttac caatagcatg gatcgttccg cttggtacat aaaagaaatc
    35701 acctttttta actggtactt tcttcaaaag gtcatcccag cgcccatctt tgatcatctc
    35761 agctagttct tctcttgtct ttgcatgatg accatagatg atttctgcac caggttcagc
    35821 atcgatgata taccagcatt cagtttttcc taattcacct tcatgtttca tcccataggc
    35881 atcatctgga tgtacttgta cagatagatc atcttctgcg tctaagatct tgatcaataa
    35941 tggaaacact ggttctgaag gatggccaaa caattccttg tgttctgacc atagttcatc
    36001 taatcttttc cctttgaatt cgccattttc tactacactg acaccatgag gatgcgcact
    36061 gatcgcccaa tcttctccga ttttatcgtt agggatatca aaaccaaaga ctgaacgtaa
    36121 acgacttcct ccccaaattt tttcttgaaa aactggtttt aaaaacatag gttcctgcat
    36181 aattcaaact cctctcattt ttcatagcta ttatagcatt atttcggtaa taccgctttc
    36241 tctttcaaaa agttttcaac aatttcatct cgacatatga gatactccgc acgatgatcg
    36301 acaggatgta cgcgatttga caaaaatatc aacgcttctt gtgtttttat atcaatcaag
    36361 aaaaacgtac ctgtataacc agtatgaaaa agaatcgcat gaccgtcttc tacatccttt
    36421 tttaagtccc atcctaaaga acgatagcca ttttttttcg gtgtttgatc ttgcagtaaa
    36481 gaatcaatcg tctcttcttc taaaaaacga ccattacgga tttttccctt gttcaaatac
    36541 atttggacaa aagtaatgct gtctttcaaa tcaataaaga gacccgcgtt acctgcatgt
    36601 tcagccaaaa ctcttgcttt cggatcatga gtttgtccac gcaaaacctc acccgaaggc
    36661 agttgttctg tgggaacgat ttttttggaa ggaaaaggaa gaaataagct gttcatcatt
    36721 cctaatggtt ccaatacttg ttcttgaaag atttccactg cagtttcttg gtaaatttcc
    36781 tctagcatca acccaagaag aatcgtaccg gcatctgtat attttacttc ttttcctagt
    36841 tgctcacctg gttctagctt caaataagcg gcgatcaatt cttcctgaga tagcttatct
    36901 ctgtttggta tccatgtttt gatatcagaa gtatgtgtca gtaaatgacg caaagtgatt
    36961 gtctgatttt caaattcagg aagatattta tgaagcggtt gatcgagttc tatctttcca
    37021 tcttctaata gacgtaaaac taatgaagtg gttgcaacga ctttcgtcag tgaggctgca
    37081 tcaaacaaca gatcttctgt catcagctgc ttttcaggaa cgacttgggc aaagccggct
    37141 gtaaatgttt ctacttgatt ttcttttatg aaggcgactg ctacacctgg ataaaatcct
    37201 tcttccatct ttttcaaaat aagctgtttc gtgtgtggat acatcttttt ctcctgcttt
    37261 tctatcagat tcttacagtc caagcttaaa gcttaatcta tgaggaatca acctttttta
    37321 cacaaaaaaa ctgcgcgaaa aaccgcgcag tttcccccgg tatactattt tctctttcta
    37381 tctctagaat aagcgatagt atgaccgttc ataaaatgac gagacatgcg acaatcgtta
    37441 agaaagaaaa agttaagcgt cgcatcgaat gtcagtttta ttcttttctt ttggctcact
    37501 gacgaaccac cattcgattc ctatagcatc atagattgtg agtactgtgt gctttttgtc
    37561 taagaagaaa tcaactgata gttgctgcaa gtgagcaatc agtccttcca tgctttcagt
    37621 atcgatcatg aatttcagaa aatcaagtcc aatcacttca tctgtaggca aatcaattgc
    37681 gcctccgcta gcttctgtca aaccaacatg gaaatctcct tcattcaata catggatgcc
    37741 ctcttcttca ttatgtacct tcaaaccaag tacatgatta agaaattggc gttcttttgt
    37801 taagttagta acatttaaat ggattttttc aaaataagaa cctgttgata gtttactgaa
    37861 tttttcttca gattttctta ataatgcttc ttgatccatt gtttctggaa tttctctttg
    37921 gtcgttctta ttctcttcat ggtaaatttc caattcattc tcttctgggt ctacgagcaa
    37981 aatccccata cgtccatgat cttctaaagc ttctttgatt ggataattca ttttacggat
    38041 acgacaaagg atatctgcca tttcttctgt gctagggata atcaatgaaa aacggttcag
    38101 ctttttgatc acccccatgt gatcattcgc tctaggactt tcttccagca gcaacatttc
    38161 acttccagct tcttctgttc ctaaaatggc taattcgttt tcttctcttt tcaaatcaaa
    38221 gccaacaacg ttcttgtaaa aatcaatcat tttatctcgg tctttcaccc gaatggctac
    38281 actttttaaa taggtggcag cccccaattg aaaatctttc atatttcttg ctcccctcta
    38341 cgtcaagctt tattttaacg atggattctt cttctttcaa gcaataactt tgattttgtt
    38401 aaaaaaaatg tgagaaaatt cgcattttgc ttgttttttg taaattaatg agtaaaaact
    38461 cttataaaaa gaggttgtga aaaagctgtt ctgcatcaag taataagaac agccaaacaa
    38521 aaatagctcc tcctattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
    38581 caccgtttaa ggaagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt
    38641 gtgaaaatgg ctttctacat ttttgcataa atttggctta ataccgagaa actggctttt
    38701 gaacaccgtt taccaagagg ttgtgagcct gccgtttaac tccgagtcac aaggagcaat
    38761 ttcaaaaaac agcttctcag gtttgttgac tattgtgact tgtgaccgag gagttggcag
    38821 gagaacaccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagata
    38881 caaaacggaa aaatagcttc tcatattttg ccgttttttg agctaatacc gaaggacagc
    38941 tgattgaaca ccgtttattc ggaaaaaggg actgtgacac gacttttgtc acagccccta
    39001 aaaaaaataa taaatttgta ctattctatg aagcttcttc ttcagaaaac tgactgttat
    39061 aaagatctgc atagaatccg tctttttcca tcaatgtatc gtggtttcct gtttctacga
    39121 tcgaaccatg attcatcaca ataatgttgt ctgcatcgcg tattgtagac aagcggtgag
    39181 caacaacgaa acttgtccgg ttttccaata ggcggttcat cgctttttga atcagtattt
    39241 ctgttcgtgt atctacacta gatgttgctt catcaaggat caggacatct ggatttgcta
    39301 agaatgcccg cgcaattgtg attagctgac gctgcccttg cgaaatattg cttgcatcct
    39361 cgtttaatac agtgttatat ccttctggca atttacgaac aaaatcgtct acgtgagcag
    39421 ctttagcagc ctcaatgatt tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt
    39481 catagatact tcctgtaaac agccaagtat cttgcagtac cattgaaaac tgtgcgcgca
    39541 attcatcacg tgaaagatca cgggtatcta caccatcgta acgaatacta cctccagaaa
    39601 catcgtagaa ccgttcaaga aggttgatca gtgtcgtctt ccctgcacca gtcggaccta
    39661 caatagcgac catctcacca ggtttgacat ccaagttaaa atcagtcatt agcaagttat
    39721 ctggtgtgta gccgaattga acattttcaa aacgaacttt gtatggtgta tctttttctt
    39781 ctggaaggcc agatttttca ttggacatct cttcttcatc caacacttca aatacccgtt
    39841 cggctgaagc aactgttgat tggatagtgt tcatcaaact tgcaagttga gtgattggtt
    39901 gagagaactg tgttgtatat tgcaagaacg cttgaacatc ccctaatgtc atgttcccat
    39961 ttgcaacttt tactccgcct agtacagcga cgaacacata gcctagattt ttaacgaaat
    40021 tcatcaacgg catgatgatt gccgaaataa attgagcttt ccaaccagcg gcatacagat
    40081 tctgattttc tttttcaaat acttcctggt ctttttcagt atgattgaat gttttaacaa
    40141 tggtatgacc accatatgtt tcttctactt ggttattcaa taaacctaaa cttttttgct
    40201 gatctgcgaa aaacacttgt gatttcggcg ctacgatcat cacgatgatc aagcttaaag
    40261 ggatcatcaa taacgcgact aacgtcaatt gccagctgat tgaaagcatc atcacgatga
    40321 ccccaatcaa tgtgactgca ctagttacaa attgagtcaa attttgctgc agcgtgcttg
    40381 cgatgttatc catatcattg attgctcgag acatgatatc cccatttgta tgtgtatcat
    40441 aataggaaat cggtacacga ttcattttgg cttccaaatc atgacgtaat tcataaactg
    40501 ttcgttggga aacacgtgtc ataataaatt gctgtaaaaa gttgaataca gcagaaatca
    40561 aatacatcac gattactgtc gcaatgatct gcgcgatttt atcaaagtct atcggaaatt
    40621 ttgttacttg ttggccttgt tgcatctgtt gagcgccagc cattacacct ttgaatattt
    40681 ctgtggttgc tttccccaag acctttggtg tttggatttg gaagatcacg gctgctattg
    40741 ctagtacaaa tacagcgatg atcgcaacca tacgtttaga catatagcca aacaatcggc
    40801 gtaccgttcc ccagaaattt ttaggctttt caccttttac accgagattt ctgccaggtc
    40861 caccatgtcc tggaccgcct gctggtccat gagagggacg tgtgtttgtt ttactcatgc
    40921 taagtcctcc tctcttagtt gtgaatgaac gatttcttga taagtttcgt ttgttgctaa
    40981 tagttcttca tgtgttcctt tgccaaccag ttttccttca tctagcacaa ggatcatatc
    41041 tgcatccatt actgtactta ctcgttgagc aactaaaacg gtgatacttt ctttcatatt
    41101 cgttttcaat gcttggcgaa gattggcatc cgttttgaaa tccaacgctg agaaggaatc
    41161 atcaaacaca tagatatctg ctgttttgac taatgcccgt gcaattgcca gacgctgtct
    41221 ttgtccacca gagaagtttc ctcctccttg ttccacatgg ctatctaagc catcttccat
    41281 ctctgaaaca aaatctttag cttgtgcgat ttctaatgct ttccaaatca tttcatctgt
    41341 tgcatccggc ttaccatact gcatattgtc tcggatcgtt ccagagaaca gaactgcttt
    41401 ttgaggaaca aagcccatta attctcgcaa attatattga gtcatgtcac taatgtccgt
    41461 tccatttatt tggatcgaac cagattcgat gtcatataat cgagggatca aattgactaa
    41521 ggtcgtttta cctgaaccag tcccccctat gattgcgacg atctcgcccg acttggcttg
    41581 gaaatcaata tcttccaatg caagtttttc tgctccatga tatcgataat tgacatgatt
    41641 gaaggccaat gttgcgtttt ttccagcaaa agaaactgtt ttaggatttt cagggtcatg
    41701 gataccgatt ttttcatcca acacttcatt aatacggtca gctgaagctt gtgcacgagg
    41761 caccatgata aaaatagcgg aaagcatcat aaagctgatc aagatctgca tcgcataagt
    41821 cataaatgca atcagattac cgacttccaa agtcatatct gctatgtagt gtccaccaaa
    41881 ccaagtgatt gctacgtttg taccgctcat gatcaatgtc atcattggca tcatcaatgc
    41941 gacgatcgta ttaactttga ttgccgtatt tgtataatct ttattcgcta aatcaaaacg
    42001 attttcttca aagcgtgtct tatcgaatgc tcgaatcact ctgacccctg tcaatccttc
    42061 tcgaaatacc aggtttaaac ggtcagtttt cttttgcata cttttgaata atggaacagc
    42121 aaagtacatg attccgccga tcaaaataat cattactggg atgacaacta agaagatttt
    42181 tgtcaactga tgatcttttt gataagcaag tatgctcgcg ccaatcaacg tgattggtgc
    42241 attgatcatc atacgtaaaa acatttgtgt aaccatttgg atttgagtca catcattggt
    42301 cgttcttgtg atcaaagagg ccgttccata tttatcaaac gcatcttttg tcagactctc
    42361 tgatttttta tagacatccg tacgcaattg cttacccagt ttctgtgatt cacgagtagc
    42421 aaaaaatgta tttgcgatag cagctaagat actgatcaaa gaaaagccga tcatcacaaa
    42481 acctgtatgc caaatataat cgacatctcc ttgcgctacc cctttatcaa taatattgga
    42541 ggtaagtgtc ggcaaatata aatctgccag tacttggatc accatgaaaa ctgcagctgc
    42601 aattgctgaa gtcaaggaga tccgttttac gatttttagc atctagttcc cttctttcta
    42661 tttatatact ccgtttttca gccaagaaag tttcaaattg aattcactga ttgcttgctc
    42721 tactgagtaa tcttcagatg ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac
    42781 agtattcatc actaattgga tcagtaattc caattcatga tcatttttta ctttcagctt
    42841 cgtccgatcg atcaattcaa gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc
    42901 gtgaggcgtt ttttggcgac tcgtatgagg agcgactttt cttgaggcac ggtaatccat
    42961 gttcataaac aagttacgat aaaaagaagc attctcgcct tccaaaatct cataaatcat
    43021 tttagaaaaa taggattcga atcctttgaa tagatcacca tcggattctt tcattagttg
    43081 taacatatct ttagagctgt cacgtctcat tgtctcaaag taataaaaat aaagatcctc
    43141 tttatcttca aaatactgat agaaactgcc tctagggata tccgctaatt tgacgatatt
    43201 agcaatcgaa gcttctttta agggtactct agaaaactcg attctcgcag cctcgattag
    43261 tcttttttgt ttttctttag gcaaatggaa aaatgtctgt gtcggcatat ggattctcct
    43321 ttctttcaag atgacacatt gtcatatgac gctatgtcat tataacacct cctacctgtt
    43381 ttgcaagaca tttgcatagt ttttttcatt atcatccgat acattaaaaa agtgataaga
    43441 ggaatccttc catttgaatt aatgagagtt gcccattaaa atagccacat actaaacaaa
    43501 ggatgagata aatggcccaa tcaattagtg tccaagactt caataaattg cctctcgatg
    43561 ctgacagcat cgttttagat acaagaaata ccttagatta cacaaaaaac cacttaagcc
    43621 actcgctttc tatccccgct gctatcttgc caagacaatt aaaaaatcta gatccaaaga
    43681 aactttatta tattctaagc tatacaggca ggcgatcaga agcattagcc gatcaactta
    43741 ctgctaatgg ttttcgtgct gtttatatca ttggtgggat ggaagctctt cagtcgagtg
    43801 ttgcttgaat attgcttgtt agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc
    43861 tcttttatac cctgtctttt tttagttatt tattcttcaa ccttatcaat atgacttgat
    43921 agccttttct ttttcgagta aactgatttt gttagtcata gttataacaa tcatagcaat
    43981 aaaaggagaa ggtaagcata tggcatctgt tttagtagtc aaaggtcatc ccctaactgc
    44041 agaagaatcg cgcacagtta aggcattgac gtctttttta acaagttata aagaaaatca
    44101 tccagatgat gaagtgactg ttttggaact ttatcgcgat gatattccgg aaatcgatga
    44161 agaattatta tcaggttggg aagcattacg tgctggtgca gaattcactt ccttatcaga
    44221 aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact gatcagttcc ttgctgctga
    44281 taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc ccaacaaaat taaaagcttg
    44341 gttcgatacc gtcaatgtag ctggaaaaac gttccgctat acagaaaacg ggccagaagg
    44401 attagtgaca ggtaaaaaag cactgcacat ccaatctaat ggcggtgtat acaacggaca
    44461 agactttgcc tctcaatatg taaaaggtat tttaaacttt gtaggcatcg atcaagtaga
    44521 tcagctattc atcgaaggaa tcgattatga ccctgaccgt gcagatgagt tgatgcaaaa
    44581 tgcattagat aaagctgctg ctttaggtaa atcattttaa aaattgagat aaatgcatac
    44641 aaaagagaca gaggaaaaaa tcttcaaagc agcatttgtg ccttggatat tttcccatct
    44701 gtctcttttt gttattcttt ctgctttttc aacggatttt cttcataaag agaagagatt
    44761 gttgtgtacc attcaaaaac atgagtaatc actactttag ctgctgcata aacaggaatc
    44821 cccaagatca caccgatgat tccgaacaat ttcccagaag tcagcagaac taataaaatc
    44881 gttactggat ggattgctaa ctgattccct agtactaatg gagagatcaa gcgtccttct
    44941 attgtctgct ctactgcaaa aacgatcagt actttgatga tcatgaatgg acctgctaca
    45001 atggctaaaa agatagccgg aatcatcgct aaaaatgatc ctaagtaagg gatcaggttt
    45061 aaaaaacccg caataatccc caacgtgaca gcatactcta aaccaatgac cgcaaaacca
    45121 atcataaaca tgattgccac agcaaaagcc acagttagtt gtcctcttat ataagaagaa
    45181 acttgactat tcatttcact cagcacattc aacgtcggtt tttgcatgcg tgtcggtaaa
    45241 aatttgacaa aataaggtgc taattgttta ccgtctttca atagataaaa taagataaac
    45301 ggcatcgtca gcagagccac taaaatagtc gctaccgcgc cgacaaaact tccaagactc
    45361 tgaactgtac tcttagatat atcttggatc atatcgcctg ccgaattcat gaacttatca
    45421 cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg ggtcccgcaa aatctcggtt
    45481 aatttattat caatcgtatc tacatattga gggaagttac tgatgaagct cactgtttgt
    45541 tcctgaatct tagggatgat cactacgctc ccccatgcga ttagtgcaac aaccaaaaca
    45601 aacagcccaa tgatactgta aagccttggt acttttcgct tttcaaagta atcaactacc
    45661 ggattcatca agtaatacaa gattcctgct aaaatgatag gcagacctac gattgctaaa
    45721 aattgccaga ttggtgcaaa caaatatgaa acttttgtaa ataaaaagac gatcagcaat
    45781 attaacaata caacaagtaa ggctgtgact acttgattat ttaaaaacca gcgccaaaac
    45841 cacgataccg gtctgttctt cttatttttt ccttccataa ttcctcctgt tagttataaa
    45901 aaatttggct tcccaaacca atagcaggca gtctatgcgg atgaagatag attccatttg
    45961 caatttgtcc ttcttctgga caagcatcaa aaatcaatga tacgtgagct actgtcttta
    46021 aattcccatt ggccatcgga ccgacataat cgatcgtata tacctgatca tcaaaagcaa
    46081 ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga tacttcttga atcaccgaat
    46141 attttcgtaa ggcagctgtt gctgttttat cgaacaaaat cagcatgggt tcttccttgt
    46201 ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat cattgcactc tctcctttaa
    46261 ttgctttcaa aaacagtata acacattctc attctttagc cgtgtggaat aattatgtga
    46321 tagaataaaa acgaaaacta tttcattaca attaggagga atcagctcat atgatcgaaa
    46381 agataatttt aggaacctat acgcgtcgcg aaagtaaagg aatctatacg atcgaccttg
    46441 atacagaaaa agaagaatta gccaaccttt cattagctgt tgaagaaaat agccctacct
    46501 atgtagcaaa aagtaaagct ggcaatcttt acacagttac tagtgtggat ggattaggtg
    46561 gtgccggtgc gtacgataag gattttcatt ttttgaatgc ggtaacagag tctggcgctc
    46621 ctttgtgtta tgtagcggtt gatgaagcac gtcaattact atacggggcg aactatcata
    46681 aaggtgaagt aaatgtctac catatattag gagatggcga actgaaagca gcagattcaa
    46741 tattccatca agaagcgaca ggtcctcata aaaaccaaga tcatgcgcat gtccactata
    46801 cggatctgac tcctgaccaa cgattagtcg tatgtgatct tggtacagat cgtgtgtata
    46861 cttatgatgt gtccgaaaac ggcaaattga atcttgtaac taccttcact gcagagccag
    46921 gaacaggccc tcgccatctt gtctttcatc caaatggaca attcgcttat ttatttggtg
    46981 aattagacag cacagtccgc gtgctttctt ataataaaga aacaggtgct tttgaagaat
    47041 tacagaaaat atcgaccttg ccagaagaat tcgacggtga aaatggaggg gcagctatcc
    47101 gcatttcaaa tgatggcaag ttcctttatg cttctaaccg aggacataac tcgattgctg
    47161 tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa gcaaatcatc tcaacagaag
    47221 gggattttcc ccgcgatttc gcactagatc caagcaataa ttttgtcgtg tgtgccaatc
    47281 agaacaccga taatctgaca ttatatcgcc gtcatcctga aacaggatta ctggaattac
    47341 tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta ttttgatcgt tagtaagaaa
    47401 aggagaatga aatgaatcag caagacgtat tagacagact acgggaagaa ctaaatattc
    47461 ctttctttga tgggaaaatc gaagataaag agtatagcga agaggaatat cagaaactca
    47521 aagccgattt acagaactac tttgaacaat atgtacagaa tgtagaaaac taatcagctt
    47581 tccatactat ctgaaaagaa catcaggaca agacttttcc gtctatcctg atgttctttt
    47641 ctacaaataa aaaaccgccg agatggttag actcgacgga aaaggagtat ttactcgatt
    47701 ttgagtaagt acaaggttgt tgttggttat aggtatgtca ttatcataaa ggattttgat
    47761 cactctatca taaaggtaat attacattta tattagataa ctctagtttt tattgttatc
    47821 aaagatttct tttatttttt caacaacaaa ttagtcattt tttggatggc agctgtcaat
    47881 tggtctgtcg cttggtcttg ttttgcttca gatgtattga atagatcttc atctacccct
    47941 gattcaagat cgatactttg gatcatttct tcacagcctg ctacatatga aagataagct
    48001 ttttcaaaat ttttatgaat gcccattgct tttgctggtg gacgcaaagt actgactttc
    48061 ataagcattg cccgatattt ttttgttcct tcttcaaata acaggatgat ctcttcaaga
    48121 gtcgatttgt ccagttcact tgtttttcct tcatcaattg cgtttctgat cacttcatat
    48181 ttcggatgca ttccttctcc gattgcttct gtttccttga cgatttgatc gatggactgc
    48241 acataatagc caatattcgg taacatgttc gttttcctac tttctccatt gttgttatta
    48301 tttattctac ttgtttttat cctcaaatga caagtacttg aaaaaattta atggtttcta
    48361 tacagctcgc cattcttatt cgtgctcaaa gaaaccatct gctaaactat attgtttccc
    48421 tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa atctcttgtt gcgagcgatt
    48481 gaattgcgct tctttatacc actgtccgtt ttgatagtac agttctcgtt caaacgccgg
    48541 aagttcctgt tctaatgata aaagcagctg ataaaagcca tttgttgtct gattcgtctg
    48601 ttccattaga ttcgctgcaa aataaaacgg actggtgatt gcatcgtgag tcgtttgctt
    48661 ttctaattct cctttactgt cgaacataag aaattgtgtt tcatgcaatg tttgtttgtc
    48721 attttttgct tggatcgtat ctgaatagat ccctggaaga tgatcccccc aaaaaacaac
    48781 taaagtcctt ctagaaagat tcttcaattc ttccgtgaac tgcttcagtg ccgtgctgct
    48841 ataggaaata tcttgcagat aattctctaa gtcaagtgtc ccacttccat ctgaagtttt
    48901 tgcaggatag tctagattat catactttcc atcgtagggc atatgtgtct gcatcgtgac
    48961 taaatgaatg aactgaggtg ttttttcttc cttgagaagt gtcattactt ctttgtaagc
    49021 tgactcatca gaaatatatg gattattctc tatcgtatcc gtataggtca tcgtacgctc
    49081 gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc tctcttttat acatcgaagt
    49141 attgtatgga tggatcgctg tcgtctggta tgattgtgcg tttaacgctg aaacgataga
    49201 aggaagctga tccatttttg gtacaagcat cgtgtaaggg gtggtgagtt gcgcattaaa
    49261 taacgccata gaaaaaccag tcaatgcttc aaattcgata tttgctgttc caccgccata
    49321 gttttgagaa agcatattgc cactatatgt ttgatcagct acttcataat aatctgccaa
    49381 tggctcacca gatacttcta cgccgtttaa tcgactagga tctgagaaac tttcactcat
    49441 cacgaacacg atgttcggcg actcttcctc tacagtttta tttttttcat cggctagctt
    49501 ctggtatttt tcagtgattt ctttgatctt ttctttcgaa tatccttcag gttcatccat
    49561 tggttctacc ttcagattgt acaaaaaccc accgataaaa ccagtgttat agtagttcat
    49621 tttctggctg taaggtatcc atagtgccgt tttattatat gcttttcgca acaaattatc
    49681 gggattgttg aaattactga tataccctag cagtccaatt gccacaagca gtgtcgttaa
    49741 tcggatcaat tggaatcgcc ttcctttttt caggcttcga aacatgtacc agcaaaacaa
    49801 tcctatcacg gttcctgcgg cagctaagat cactgtaaac agcatagtgc cggtcatatc
    49861 tttcagcaat ccgatttcag tgatcatctt caaatcgtct ggataaatcg gttcttgccg
    49921 ataactcatt ttcatataat ttgcaaaacc taatacaccg atggctacga catacgtaag
    49981 ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa ataagaataa tcagcaagac
    50041 gagacaggct aagaagaatt tttccgtatg ccaagaaaaa gcaaatttca acgcaaggtc
    50101 tacggataga tcattttgac accactgcaa aaataaatta ctaaaaacaa caagaaaaaa
    50161 gatactgatg attgctgctg ttccttttaa aatctttttc tgtttggtcg aaagtctgct
    50221 tatatatctt ttcaaatgct tgctccttca tttctatcaa taaaagaatg ttgcttctct
    50281 tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag aagaattaat gaaatccaaa
    50341 tattctcttt ctttatggcc tttctggttt ctttaataag cggacctttc gtactccaga
    50401 aattttttct agctgttgtt tcacttgatt gagctgttca aaatcagtac tgtctatgtc
    50461 aatcaatatg tagacatgct cattcttatg gttacgcgat atatctgcga tcgctagctc
    50521 gttctcattc agcagagaaa atatctctgc taatatattg gttttctttt gataaaatat
    50581 agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat ggataattga ccgcttctct
    50641 aaccgtaccg aacaaaagat aatcttttaa ggcttcaact gccaaacgtc cgctgtctgc
    50701 caaagcttcc tgagtcgatc cccctagatg aggcaacata agtatacggt cattttctaa
    50761 gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt ccaatgcttg
    50821 cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta cagcatcttt
    50881 tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg tcaacggcag
    50941 catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt gccgaacata
    51001 atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc tatagcaact
    51061 tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt tgccttgcaa
    51121 ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat taggacctgt
    51181 tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga gcaaacagca
    51241 caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga cgatcgtccc
    51301 gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc ttgaaattgc
    51361 tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa ctattccttc
    51421 ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta tctcttgttc
    51481 tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact aacaagtatc
    51541 tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc aaaaagagca
    51601 gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact gccataagcc
    51661 ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt tccgttagct
    51721 gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt caatctcttc
    51781 ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa ccactgtttc
    51841 caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta gcgagacaac
    51901 ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga aattcggttt
    51961 agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg gttcaaaggc
    52021 ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg atacgactac
    52081 cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat ctcccattga
    52141 taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta cttccatcat
    52201 atgggattcc aagattgctt cttggccact agctagctcg taagtataat cttccatgaa
    52261 tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg cagttttcca
    52321 gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag ctagtccagg
    52381 tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat agcctccttc
    52441 ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat gttcctccca
    52501 tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact cttcaaataa
    52561 ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc cgataccaaa
    52621 ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt ctgtgacacc
    52681 cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt cattataggc
    52741 tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt tccagtagcc
    52801 aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt cgcggtcacc
    52861 atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag gaatgctttc
    52921 gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc cgcggatcca
    52981 catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt cttctcgata
    53041 attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt cttgaaggcg
    53101 gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt gtgcatgttt
    53161 ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa caaaccaaaa
    53221 ttctttttct ccaatattca acataattag ccactccttt tatttgtttt gtccgtaata
    53281 cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag gcagctcttc
    53341 tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat ctagtacgac
    53401 tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt gtccgtgtaa
    53461 aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt ctatgataac
    53521 ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa atcgagcaca
    53581 aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa catccaatcc
    53641 tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc cgatttgagg
    53701 aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga gtttaccttc
    53761 aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct cttcataact
    53821 gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc tcacatttcc
    53881 ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt atacttcttc
    53941 tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga atcgctgttt
    54001 tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct gctatttctt
    54061 ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct gccaagaagt
    54121 tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg attccccacg
    54181 ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca gatgctagaa
    54241 tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa cgatcaattt
    54301 ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa gcactggcta
    54361 aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt acaaataatg
    54421 gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa gaaagtaatc
    54481 ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat aatttatctc
    54541 tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc caagcattga
    54601 tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa ggtgtagtga
    54661 ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc atagcgaagg
    54721 cggaagtacc agccgaaatg ttccctgtcc ctttggctac actgttcgta gcgaccatac
    54781 cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa tttcccgtag
    54841 ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct gctactagaa
    54901 ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt aactgaccaa
    54961 aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga aacatgcctg
    55021 atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca taacctgcaa
    55081 gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata gattgataca
    55141 aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt aatattcgtt
    55201 ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac tgttccttgc
    55261 tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg attgtcgtaa
    55321 gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg acgcgcagtc
    55381 ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg ttttcccact
    55441 cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct ttgatcctag
    55501 ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac atactttgcg
    55561 catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga tcaaaccgtc
    55621 tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc aagcagttat
    55681 ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa aatgaaattg
    55741 cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata cttatgaaaa
    55801 aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc ggcattctat
    55861 ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat gattggggtt
    55921 tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta ggtgcgatcc
    55981 ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa atgattcttc
    56041 tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct cacaatggtt
    56101 cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct gcatctgccc
    56161 tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga ttgtcaggga
    56221 tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat tatttgctaa
    56281 aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct gtccccgcgc
    56341 tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt atgcaatctg
    56401 gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa gaagctgaac
    56461 aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga acttcttggc
    56521 atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt gtagcagtat
    56581 tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc gtagaaaaag
    56641 cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga atcattttag
    56701 ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga actttattga
    56761 aaataggcgg tagcgtgatg ggactttctt tcatcctacc agctgtttta ggaactgtac
    56821 tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat gtcgcatttt
    56881 attcttgcac atgggctcca ctgacatggg taatcgtagg cgaaatattc ccactagctg
    56941 tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct ttcctagttg
    57001 gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc ggcatttttg
    57061 ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa actcgtggga
    57121 aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa cgtatttcta
    57181 tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaacc
    57241 gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt tgatgaatat
    57301 tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt gaaagaaacg
    57361 ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac cgtaggagca
    57421 ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt tcccgttcaa
    57481 ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc gttgtgacga
    57541 tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt gtcaattttt
    57601 tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg tcatctggaa
    57661 tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga tcgttatagc
    57721 aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt tcagcgattg
    57781 tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc acattatact
    57841 gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg tcgatttttg
    57901 tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc ataaaaccga
    57961 ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca gcattgatca
    58021 tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt gggttatact
    58081 gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc attttcttca
    58141 aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat gaatacccag
    58201 attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa tcagagagat
    58261 atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct ttcttatagc
    58321 tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca tttaccaaca
    58381 cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc agcttgaatt
    58441 ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt tttatctctt
    58501 ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac gtacatatat
    58561 cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc tcataaggca
    58621 tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa gcattgcgcg
    58681 ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc tggtcgagca
    58741 tgatagatca aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt gtgaccagga
    58801 ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt atgccatgat
    58861 tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc atagttttca
    58921 tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag gactgctgga
    58981 ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga aaggaggatc
    59041 tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt agaattccca
    59101 gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga agagaaagtt
    59161 gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc tatccaatct
    59221 gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg atgagattca
    59281 tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc attgatataa
    59341 tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc ctgccataca
    59401 atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa ttcaatcact
    59461 tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt atatacccaa
    59521 ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat aacaaatttc
    59581 ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct acatttaatt
    59641 cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct ggttcttcac
    59701 gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct tgatctagca
    59761 aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg atcaagaaaa
    59821 tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca ttttttatct
    59881 tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct ttagcatatt
    59941 tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct ttagcatctg
    60001 tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt acgcgatctg
    60061 cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc atagtaaagt
    60121 aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc ggttgggcat
    60181 gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa tatcctttta
    60241 cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa gcttttgcaa
    60301 ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct tcttcatcat
    60361 acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct tcttctaatg
    60421 cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca actaagattg
    60481 tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct aacctaccat
    60541 attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc cctaaacacc
    60601 ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca ctccagaagg
    60661 ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt ccttttgttc
    60721 ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta aaaaattcgt
    60781 ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt tttggtcgtt
    60841 gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt cctccaggat
    60901 agcgaatgac acctaattct aattccttta cataagagag gacatcttgt ctaaacccgt
    60961 caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca cttcccatat
    61021 gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa tcctctctga
    61081 tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct atagtcaaga
    61141 taatagatac aagtttccat acgccaccag caaagataaa aataaaagga gggaaaagaa
    61201 atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc aaaaaatcaa
    61261 aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct ttgttttcag
    61321 aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact acatatccac
    61381 taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa taaataatga
    61441 tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct tgaaagaaaa
    61501 aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa aaaacagcac
    61561 atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga atacctaata
    61621 agatgtaatc agaaaccaca atcaactgtc cataaagttt agtctcaact attttcatct
    61681 tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct gtgcaaaaat
    61741 ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag gtccataaaa
    61801 agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca tttttgaact
    61861 acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg tgaaaatccc
    61921 taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa tattaaactc
    61981 acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa taaaaaagga
    62041 acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac ctgcataagt
    62101 atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga cttggaatgg
    62161 gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga aacgcatacg
    62221 agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg tccctccagc
    62281 aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa ccttaatata
    62341 gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa tgtctttttc
    62401 gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca acaatataac
    62461 tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac tattcacagt
    62521 aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt tgtttttctt
    62581 tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata aacaaaaccg
    62641 aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga ctgtaaatat
    62701 attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca tatacttggt
    62761 caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag gagggaacga
    62821 tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc atttgagcag
    62881 attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc atagtaacac
    62941 caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt ggatcgcgaa
    63001 aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc cctacattcg
    63061 gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata actggaatgt
    63121 aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct actgctgcta
    63181 ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa gaatgaatca
    63241 acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa cttacaaaat
    63301 ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt cctaaaagcg
    63361 gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa tacgcagcac
    63421 gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca tctttcattt
    63481 tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa caatcgctgt
    63541 gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata tctgtatctt
    63601 gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga ttgatggcat
    63661 catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt tcaatcagtg
    63721 gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa tatggctctg
    63781 catagtccat ttgtttcgat aattcagata caataggatc attttttact tcttcatctt
    63841 ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt ttaccaattt
    63901 cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta ttatctgtac
    63961 ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct gaatcactcc
    64021 cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg ttatttaacc
    64081 atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc atttcggcgc
    64141 cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc gcttttaacg
    64201 ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc actaatttac
    64261 cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg aagacaaaac
    64321 ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat tcttcccaag
    64381 tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta ttccaataca
    64441 tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact tcacctaatt
    64501 caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag aaatcgtcta
    64561 atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt aatgcaataa
    64621 agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa ttggcccaag
    64681 tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat tcattaacga
    64741 tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc aaagtcactt
    64801 ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa atacctgttg
    64861 cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt tcctccttca
    64921 tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca attgattttc
    64981 ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact tttaggaaga
    65041 ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc ctctaatgat
    65101 aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac tgcaaatatg
    65161 acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc ttcaaaatct
    65221 tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg tccatacaca
    65281 gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt tgtttctgtc
    65341 ctgattggtg caataacatt taccaattga gcaagacaag caatctttac tctatctgca
    65401 tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa attatagtta
    65461 tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg ttccgctgaa
    65521 tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact atgtttcttc
    65581 gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat gtctaaggaa
    65641 cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg cagagacaaa
    65701 taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc aaatgtaggc
    65761 atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac gagtttcatc
    65821 gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg tccaatctgc
    65881 caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg gctctcttga
    65941 ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca atattcgact
    66001 aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt gatttcggcc
    66061 cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt ttcaattgtc
    66121 cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact gtcttcccaa
    66181 cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt caattctttt
    66241 accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg atctggctga
    66301 taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta caaccgcgga
    66361 tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat aactgacctc
    66421 ctttaattta tacatgaata atacacctcg ttgaaagcgt tgtcaataat ataaatgtta
    66481 aacttactta ttttttatcg attttaatta accaaaatat taataatgat taaaacaaat
    66541 aatttaatat aacttttatt aacaaatatg ttaatatata agaaaaacct tttaagaggt
    66601 gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag cattagctag
    66661 taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg tcaaagattt
    66721 ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa agctagagga
    66781 agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc ttcgtgtcga
    66841 taaaatcgat atcagttttc cagaaaaaat attcaatgct tttgatacga aagaaacatc
    66901 gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt tagctacgat
    66961 ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc gaaggatgga
    67021 tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta attttttaaa
    67081 tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg aatttccttt
    67141 ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg agctaggaac
    67201 atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg attggtatcc
    67261 agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac acttgacgaa
    67321 tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag agcaagacac
    67381 ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct gtactttatt
    67441 tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact acagctgata
    67501 tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga cggctgtttt
    67561 tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt cgtttatgtt
    67621 gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact tcttttcctt
    67681 ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct gcaatcattg
    67741 gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc ggtgcattca
    67801 atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc aaaatgtcgg
    67861 ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca tgatctgtcc
    67921 ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata cgttgacgtg
    67981 ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt aatacttgta
    68041 cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc tggataaagt
    68101 caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga cgaacagcga
    68161 taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt agttgcgcca
    68221 aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa gttttaagaa
    68281 acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt tcttcttttg
    68341 gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg ctcatttatt
    68401 ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt ccatgatagc
    68461 cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg gattcaagtc
    68521 tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt tgatttcgtt
    68581 tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta aaactgggcg
    68641 agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga acatgaagat
    68701 agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt cagatttccc
    68761 atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta ctttttctgc
    68821 attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga tcgatactag
    68881 cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg tcccaacacc
    68941 ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt catccaagtc
    69001 attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt cccgaacacg
    69061 cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt tgattttgtc
    69121 acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg tatcataagt
    69181 gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg cttcataatc
    69241 atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta tcccaaatat
    69301 cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg ctctagttcc
    69361 ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata gtggatcttc
    69421 tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga aattgaccat
    69481 attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca cttggattaa
    69541 tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg ctttaaattc
    69601 aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac gcaagtaagc
    69661 aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt ccaatcctgc
    69721 aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa aaacagcatg
    69781 taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat atagatccgt
    69841 atgcagcgtc aaactttcgt ctgggtaagt attattcatc ttgtattact cctcttggca
    69901 agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa tcttccattt
    69961 tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc catctgctaa
    70021 aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt ttattcaatg
    70081 tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa ataagtagat
    70141 tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata accaaatgtc
    70201 tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc cttttttgct
    70261 tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg gaaaggcgaa
    70321 ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa
    70381 aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg cgagacatca
    70441 atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta ccataaagat
    70501 gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca ctacaatatc
    70561 tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca ctcctacatg
    70621 gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt cgatttcttg
    70681 ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat atgtgtctac
    70741 caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag ccgtattccc
    70801 aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac tattcacagg
    70861 tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga tccaatctta
    70921 agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc tttaaagaca
    70981 aaaggattta ccttttatca cttaatagta taacataatt tcgtgagaaa aaaatacaat
    71041 tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg aggaaaaata
    71101 atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag aaccttgcgc
    71161 ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg ttatcggatt
    71221 tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac aatggcattc
    71281 aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca gaaagcttta
    71341 atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct cttgacaact
    71401 gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt
    71461 gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca agagattcga
    71521 caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa tatggataaa
    71581 gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct cactctttac
    71641 ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca taagaagtgg
    71701 ttctcttaca gctggcctga atatcagcca aaagcgcata aaggactagg aatgatgtac
    71761 gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa
    71821 gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa caaaaaagta
    71881 cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt atgctaactt
    71941 ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat attcatcgtt
    72001 gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac aattgaaatg
    72061 ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga agctcgctga
    72121 tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag ctttgcaaga
    72181 tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc tctttaaaaa
    72241 agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca agtgattcat
    72301 caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta taacttagcc
    72361 tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc aaataagtaa
    72421 gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt tttcccttga
    72481 tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca tctagtgtat
    72541 tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct tcaagatacc
    72601 ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa
    72661 acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg gtataaggca
    72721 gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt gcattaggga
    72781 tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca tctacatatg
    72841 gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc tttgcgatat
    72901 ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt gtaaatagtc
    72961 taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg aaaaactgat
    73021 ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca ttggtatttc
    73081 ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg atcccttgaa
    73141 ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta cataagtatt
    73201 cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc tcatagcaag
    73261 gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa gtctcttatt
    73321 ttaaattgaa tacattataa tgatactgta aatatatctt tattattagt gaatg
//
LOCUS       DADXTH010000005        61533 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.005, whole genome shotgun sequence.
ACCESSION   DADXTH010000005 DADXTH010000000
VERSION     DADXTH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.005"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IWI97_000306"
     CDS             complement(59..187)
                     /locus_tag="IWI97_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBC2461845.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWI97_000307"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWI97_000307"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBC2461846.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IWI97_000308"
     CDS             complement(1674..2729)
                     /locus_tag="IWI97_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBC2461847.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWI97_000309"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWI97_000309"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBC2461848.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWI97_000310"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWI97_000310"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBC2461849.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWI97_000311"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWI97_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBC2461850.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWI97_000312"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWI97_000312"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBC2461851.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWI97_000313"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWI97_000313"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBC2461852.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IWI97_000314"
     CDS             10491..12050
                     /locus_tag="IWI97_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBC2461853.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IWI97_000315"
     CDS             complement(12161..14092)
                     /locus_tag="IWI97_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2461854.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWI97_000316"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWI97_000316"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBC2461855.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IWI97_000317"
     CDS             complement(15021..15773)
                     /locus_tag="IWI97_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBC2461856.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IWI97_000318"
     CDS             complement(15932..16141)
                     /locus_tag="IWI97_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461857.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IWI97_000319"
     CDS             complement(16152..17480)
                     /locus_tag="IWI97_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461858.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="IWI97_000320"
     CDS             17616..17771
                     /locus_tag="IWI97_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461859.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="IWI97_000321"
     CDS             complement(17845..18432)
                     /locus_tag="IWI97_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBC2461860.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWI97_000322"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWI97_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBC2461861.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IWI97_000323"
     CDS             complement(20012..21301)
                     /locus_tag="IWI97_000323"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBC2461862.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IWI97_000324"
     CDS             complement(21455..22387)
                     /locus_tag="IWI97_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461863.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IWI97_000325"
     CDS             complement(22544..23203)
                     /locus_tag="IWI97_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBC2461864.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWI97_000326"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWI97_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBC2461865.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IWI97_000327"
     CDS             complement(24244..24534)
                     /locus_tag="IWI97_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461866.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IWI97_000328"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IWI97_000328"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBC2461867.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IWI97_000329"
     CDS             complement(26821..28176)
                     /locus_tag="IWI97_000329"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBC2461868.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IWI97_000330"
     CDS             complement(28200..29369)
                     /locus_tag="IWI97_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461869.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IWI97_000331"
     CDS             complement(29366..30253)
                     /locus_tag="IWI97_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBC2461870.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWI97_000332"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWI97_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBC2461871.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="IWI97_000333"
     CDS             34320..34841
                     /locus_tag="IWI97_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBC2461872.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="IWI97_000334"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="IWI97_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="IWI97_000335"
     CDS             complement(36284..36940)
                     /locus_tag="IWI97_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461873.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWI97_000336"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWI97_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBC2461874.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="IWI97_000337"
     CDS             complement(38477..39475)
                     /locus_tag="IWI97_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBC2461875.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWI97_000338"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWI97_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBC2461876.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="IWI97_000339"
     CDS             complement(40491..41222)
                     /locus_tag="IWI97_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBC2461877.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="IWI97_000340"
     CDS             complement(41226..42491)
                     /locus_tag="IWI97_000340"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBC2461878.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWI97_000341"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWI97_000341"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBC2461879.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWI97_000342"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWI97_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBC2461880.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="IWI97_000343"
     CDS             47833..49335
                     /locus_tag="IWI97_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBC2461881.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="IWI97_000344"
     CDS             49337..50074
                     /locus_tag="IWI97_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBC2461882.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWI97_000345"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWI97_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBC2461883.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWI97_000346"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWI97_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBC2461884.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWI97_000347"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWI97_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBC2461885.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWI97_000348"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWI97_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBC2461886.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="IWI97_000349"
     CDS             complement(55574..56740)
                     /locus_tag="IWI97_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBC2461887.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="IWI97_000350"
     CDS             complement(56756..56983)
                     /locus_tag="IWI97_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBC2461888.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57370..57626)
                     /locus_tag="IWI97_000351"
                     /pseudo
     CDS             complement(57370..57626)
                     /locus_tag="IWI97_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57969..58992)
                     /locus_tag="IWI97_000352"
                     /pseudo
     CDS             complement(57969..58992)
                     /locus_tag="IWI97_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(59476..59637)
                     /locus_tag="IWI97_000353"
     CDS             complement(59476..59637)
                     /locus_tag="IWI97_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461889.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            complement(59630..60304)
                     /locus_tag="IWI97_000354"
                     /pseudo
     CDS             complement(59630..60304)
                     /locus_tag="IWI97_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            60827..61417
                     /locus_tag="IWI97_000355"
     CDS             60827..61417
                     /locus_tag="IWI97_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBC2461890.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT        17077 a        13211 c        10646 g        20599 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccaaacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt ttttaagtta
    59401 caataattat ctatttaata gtcttaattc attaaaataa aaaactcaac tttacaagga
    59461 tatggtttaa aaaatttatt tatccaaccc aactagtttt cgctgcttac gatttgcttc
    59521 atctgctacg taattcatta ctgttttaaa ttctttcgct gaggcgtttt ctgataaatc
    59581 agcgatactt ttattatact tcttcacgat ttgttgtact tgttctttct cagtcatgta
    59641 attcctcctc caactctttt gaagtatatt gtattggtag tactaattga atattatctg
    59701 ctgcataatg cacttcagaa atgtcgtttt gataatcctt aatcattatg tgaaggacta
    59761 cttcaggatg tctatttttg actttttcaa tattctctct ggacttaaaa aacgcattga
    59821 taaacatttc ttttggcaca tgacgtcctt cagctagttc acgttttcga gtgaaatccc
    59881 aggcaacttt gggatcttgg taaacataga aaatcgtaac gcgaaagtta tttttaaggg
    59941 ctcgaataat atttttctct gcactaagga tagcaaaagt atcctctagt atgaaagaat
    60001 agccatgttc cagtacatat tttaatgatt gttcaactaa ccaagaagaa gctttctgaa
    60061 aaacacttga gttctttcca ttgtattctg gaaattgtgc tctaaaatag tccgcatcaa
    60121 tcacaacatg gttgtcataa ttttaagcta aacctagtgc aacctctgtt ttacctgctc
    60181 ctgggcttcc agccatgaat accgcatctt tttcatcatc agggattttt cccttgatga
    60241 ttgtttcaat aaatttatct ttattgaccc tagcgtaatc taaatacttg tctttaggtt
    60301 ccaagattgc accttctttc cttcttctat attcatacta attatacgat atttatataa
    60361 aaaatataac ggtcatttta accatattaa catttagtca cagtaatagc agatatgctc
    60421 gcaaaatgag atatgaatgt acgcagaatc cttgttttct acggcattcg cttttttttc
    60481 attggactag ttatatattt ctagggttgt tttcatgtct gagtgcccga tatgatttat
    60541 ttttcgtttc tataattact ttttgccttc cagtttcttt taagattgta ctcaagcaag
    60601 tgggaattgg ttcttttgtc atccatgtat gcaaaatgtt ataatgctgg taaacaaaaa
    60661 tgattacctg tcatttttat ttagttcgta tttaattaag ttgactgtct gtatactctg
    60721 tctaaaactt aagaattgtt gttttcgctg tcgctatcca aatttaatga atgtctgtat
    60781 gttaaaactg ttgtccttgt catagatagt aaaggagagc ttgaaaatga aaactgcgat
    60841 tacgtttcta ttagaggaat atgccgattg ggaaggtgct tatattacta gcacactaaa
    60901 ccaaaaagag ggatggcaaa acaaaactgc ttctatccaa tcagaggtca cttccattgg
    60961 aggattaaaa acaaaaatcg attacttaat cgaacaaata cctgaaaaag ttgacctttt
    61021 tattatgatc ggtggcaatt catgggacat aaaaaatcat catttatatc aaataatcga
    61081 taaattgtta aaaacagacg taccagtagc tgctatttgt ggggcagttg attatttagc
    61141 tcagagcgga ttattgcaag gttacaaaca caccggaaat gcgcaatttc cttggttaga
    61201 atcggaaaat tatcagaata aagaagattt tatagaacaa caagctgtga aagatcgtaa
    61261 tttgattact gcgaatggaa cagctcctat agaattcaca gacttgattc ttcaagcagt
    61321 ccattttgaa acagaagaaa taagagaaaa aaagattcgt ctatacaaac ttggattcta
    61381 tgattactat aaaaagtacg gagatccttt ctcctaatca ttctcttcaa ttaaatactc
    61441 cttatcaata gtatgacaag taattttact gatggttatt tgtcacactt tttctgacat
    61501 ttatcaaaga agatacactc tatttatatt cat
//
LOCUS       DADXTH010000006        59618 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.006, whole genome shotgun sequence.
ACCESSION   DADXTH010000006 DADXTH010000000
VERSION     DADXTH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.006"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            189..419
                     /locus_tag="IWI97_000356"
     CDS             189..419
                     /locus_tag="IWI97_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBC2461891.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="IWI97_000357"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="IWI97_000357"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBC2461892.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="IWI97_000358"
     CDS             1830..2006
                     /locus_tag="IWI97_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBC2461893.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="IWI97_000359"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="IWI97_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBC2461894.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="IWI97_000360"
     CDS             2622..3089
                     /locus_tag="IWI97_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2461895.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="IWI97_000361"
     CDS             3174..4058
                     /locus_tag="IWI97_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2461896.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="IWI97_000362"
     CDS             4172..4408
                     /locus_tag="IWI97_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBC2461897.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="IWI97_000363"
     tRNA            4496..4576
                     /locus_tag="IWI97_000363"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="IWI97_000364"
     tRNA            4583..4654
                     /locus_tag="IWI97_000364"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            4779..4949
                     /locus_tag="IWI97_000365"
     CDS             4779..4949
                     /locus_tag="IWI97_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461898.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5169..5876
                     /locus_tag="IWI97_000366"
     CDS             5169..5876
                     /locus_tag="IWI97_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2461899.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="IWI97_000367"
     CDS             complement(5935..6579)
                     /locus_tag="IWI97_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBC2461900.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="IWI97_000368"
     CDS             6705..6992
                     /locus_tag="IWI97_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461901.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="IWI97_000369"
     CDS             7100..7420
                     /locus_tag="IWI97_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2461902.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="IWI97_000370"
     CDS             7501..8814
                     /locus_tag="IWI97_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBC2461903.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="IWI97_000371"
     CDS             8811..9530
                     /locus_tag="IWI97_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBC2461904.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="IWI97_000372"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="IWI97_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBC2461905.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="IWI97_000373"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="IWI97_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBC2461906.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="IWI97_000374"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="IWI97_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBC2461907.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="IWI97_000375"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="IWI97_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBC2461908.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14967..15860
                     /locus_tag="IWI97_000376"
     CDS             14967..15860
                     /locus_tag="IWI97_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2461909.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="IWI97_000377"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="IWI97_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBC2461910.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="IWI97_000378"
     CDS             17797..18654
                     /locus_tag="IWI97_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBC2461911.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="IWI97_000379"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="IWI97_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBC2461912.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="IWI97_000380"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="IWI97_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBC2461913.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="IWI97_000381"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="IWI97_000381"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBC2461914.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /locus_tag="IWI97_000382"
     CDS             21410..22168
                     /locus_tag="IWI97_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBC2461915.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="IWI97_000383"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="IWI97_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBC2461916.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23095..24126
                     /locus_tag="IWI97_000384"
     CDS             23095..24126
                     /locus_tag="IWI97_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBC2461917.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24123..24890
                     /locus_tag="IWI97_000385"
     CDS             24123..24890
                     /locus_tag="IWI97_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBC2461918.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24990..26558
                     /gene="liaX"
                     /locus_tag="IWI97_000386"
     CDS             24990..26558
                     /gene="liaX"
                     /locus_tag="IWI97_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBC2461919.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26855..27175
                     /locus_tag="IWI97_000387"
     CDS             26855..27175
                     /locus_tag="IWI97_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBC2461920.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27175..27525
                     /locus_tag="IWI97_000388"
     CDS             27175..27525
                     /locus_tag="IWI97_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBC2461921.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27692..28630
                     /locus_tag="IWI97_000389"
     CDS             27692..28630
                     /locus_tag="IWI97_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBC2461922.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28645..29475
                     /locus_tag="IWI97_000390"
     CDS             28645..29475
                     /locus_tag="IWI97_000390"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBC2461923.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29519..30544
                     /locus_tag="IWI97_000391"
     CDS             29519..30544
                     /locus_tag="IWI97_000391"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBC2461924.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30559..31497
                     /gene="galU"
                     /locus_tag="IWI97_000392"
     CDS             30559..31497
                     /gene="galU"
                     /locus_tag="IWI97_000392"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBC2461925.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31622..32062
                     /locus_tag="IWI97_000393"
     CDS             31622..32062
                     /locus_tag="IWI97_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBC2461926.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32065..32667
                     /locus_tag="IWI97_000394"
     CDS             32065..32667
                     /locus_tag="IWI97_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBC2461927.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32726..33832)
                     /locus_tag="IWI97_000395"
     CDS             complement(32726..33832)
                     /locus_tag="IWI97_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBC2461928.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34042..35043
                     /gene="ccpA"
                     /locus_tag="IWI97_000396"
     CDS             34042..35043
                     /gene="ccpA"
                     /locus_tag="IWI97_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBC2461929.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35380..37725)
                     /locus_tag="IWI97_000397"
     CDS             complement(35380..37725)
                     /locus_tag="IWI97_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBC2461930.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38004..38906
                     /locus_tag="IWI97_000398"
     CDS             38004..38906
                     /locus_tag="IWI97_000398"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBC2461931.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39119..40894
                     /locus_tag="IWI97_000399"
     CDS             39119..40894
                     /locus_tag="IWI97_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBC2461932.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40960..42546)
                     /locus_tag="IWI97_000400"
     CDS             complement(40960..42546)
                     /locus_tag="IWI97_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBC2461933.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42797..43021
                     /locus_tag="IWI97_000401"
     CDS             42797..43021
                     /locus_tag="IWI97_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBC2461934.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43163..44914
                     /locus_tag="IWI97_000402"
     CDS             43163..44914
                     /locus_tag="IWI97_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2461935.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44914..46698
                     /locus_tag="IWI97_000403"
     CDS             44914..46698
                     /locus_tag="IWI97_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2461936.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47024..47875
                     /locus_tag="IWI97_000404"
     CDS             47024..47875
                     /locus_tag="IWI97_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2461937.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47929..49680
                     /locus_tag="IWI97_000405"
     CDS             47929..49680
                     /locus_tag="IWI97_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2461938.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49702..49854
                     /locus_tag="IWI97_000406"
     CDS             49702..49854
                     /locus_tag="IWI97_000406"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461939.1"
                     /translation="MLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDA
                     LITALT"
     gene            complement(49894..50796)
                     /locus_tag="IWI97_000407"
     CDS             complement(49894..50796)
                     /locus_tag="IWI97_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBC2461940.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51027..52430
                     /locus_tag="IWI97_000408"
     CDS             51027..52430
                     /locus_tag="IWI97_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBC2461941.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52430..53863
                     /locus_tag="IWI97_000409"
     CDS             52430..53863
                     /locus_tag="IWI97_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBC2461942.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            54008..54934
                     /gene="trxB"
                     /locus_tag="IWI97_000410"
     CDS             54008..54934
                     /gene="trxB"
                     /locus_tag="IWI97_000410"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBC2461943.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55148..56875
                     /locus_tag="IWI97_000411"
     CDS             55148..56875
                     /locus_tag="IWI97_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBC2461944.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56890..57240
                     /locus_tag="IWI97_000412"
     CDS             56890..57240
                     /locus_tag="IWI97_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBC2461945.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57332..58192)
                     /locus_tag="IWI97_000413"
     CDS             complement(57332..58192)
                     /locus_tag="IWI97_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBC2461946.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58284..58712
                     /locus_tag="IWI97_000414"
     CDS             58284..58712
                     /locus_tag="IWI97_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBC2461947.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58874..59050
                     /locus_tag="IWI97_000415"
     CDS             58874..59050
                     /locus_tag="IWI97_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBC2461948.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59077..59523
                     /locus_tag="IWI97_000416"
     CDS             59077..59523
                     /locus_tag="IWI97_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBC2461949.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        19420 a        10339 c        12778 g        17081 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaagcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgac gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagtttc aggaggtctc tttgacttag atcagctgga agaagacatc gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgacagcga agccgcacaa aaagtgatca
    13261 gtgaaaataa cgctaataaa gaaacctatg accaattcca ttctcttgcc gaagaactag
    13321 aagaaatcga attgatggtg gagatgctgc aagaagaacc tgatgcagca atgcaagaag
    13381 aagcagaaac acgtatcaat gcattagatg aaaaaatgaa gacgtatgaa ttatctatgc
    13441 tgttagacgg accttatgat cgaaacaatg cgatcgtcga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagactgg ggcagcatgc ttttacgtat gtatacacgt tgggcagagc
    13561 agcatggcta tcaagtagaa acactagatt atcaagcagg tgatgaagca gggatcaaga
    13621 gtgtgaccct gctgattaaa ggacataacg cttttggtta tctaaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacggatt tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gctcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatcccta ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct cgattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gagatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tcgtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacgttgact ttagtcggtg tattcatggc agttatcatg aatgcaacta
    15121 aattagctca ggatatagaa ggaaacgttg atgtatctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagtaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaggaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat ggctgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaagcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaaatc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatatctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctgatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactgatt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcatttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacacaaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgaggattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgtg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta ttgttgccac accatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggacag ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta
    19321 aaggcagtac cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggacttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatgtagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctgacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcattgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggctttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggtttca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgatcat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcggctcg tatcagcgac tatactgcct ttttctatct
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 aattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagt ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cgggagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaaaat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 taaagcggtg attctgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa atagctcctc atatttttca
    22981 tttggctgga cttattaccg cagatgcaag cttttgaaca ctgtttattc gggaaagggt
    23041 ttgtgacaaa atatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa
    23101 aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag
    23161 catcaagctg ctttatatga agtacctgtc atcgagatcg aaaaagtacg aataaaagag
    23221 gatcatcaat tgattacagg aagactgatg aacacgaatc aaaaaggaga atcgatttat
    23281 ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg
    23341 tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg
    23401 cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggcgg aaaacatggt
    23461 ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc
    23521 tttactaaaa tagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg
    23581 atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca
    23641 gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatctttgg
    23701 aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt
    23761 tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc
    23821 attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg
    23881 ctttcgggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc
    23941 gtctatatca gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc
    24001 aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta
    24061 ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa
    24121 cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg
    24181 gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcttgt
    24241 tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat
    24301 tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt
    24361 ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt
    24421 tagcaatcca aggttttaca acgatagaac tagaagaatt atctggctta gataagactt
    24481 tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg
    24541 ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc
    24601 aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt
    24661 caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt
    24721 ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat
    24781 atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga
    24841 ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac
    24901 ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc
    24961 aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag
    25021 tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg
    25081 aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa
    25141 aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc
    25201 cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg
    25261 aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg
    25321 ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag
    25381 aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa
    25441 tcaaagagtt agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag
    25501 aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt
    25561 ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa
    25621 aaatgtcaga agctggatca cagttaggaa aattcttgaa gaaaacattc caaactgttt
    25681 cagaaacagt gaatgacaac atggaatgga aagatgtcag cttgcgtgtt cctgggattg
    25741 cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata
    25801 tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg
    25861 aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac
    25921 ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc
    25981 gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc
    26041 taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga
    26101 atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg
    26161 gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta
    26221 cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac
    26281 gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg
    26341 tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa
    26401 gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc
    26461 aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga
    26521 caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc
    26581 agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc
    26641 cttattgccg ataggctatc tcttgaacac cgtttaccaa gagattatga acttgccaat
    26701 tatcgccgag tcacaaggaa taatttcaaa aaaagctcgt cacgttttat gaatatggta
    26761 atttgtgacc gagaattttg caaaaagaca ccgattaaga agaaggttgt cacaacgatt
    26821 ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc
    26881 caataaaaat gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga
    26941 tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat
    27001 cattttatac attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta
    27061 tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac
    27121 aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg
    27181 tactttcaac gtattttagt taatacgtta acattcatat cattggctgt tctttttcct
    27241 gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg
    27301 ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca
    27361 ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg
    27421 ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg
    27481 tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg
    27541 aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat
    27601 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
    27661 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
    27721 agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat
    27781 cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta attattattc
    27841 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
    27901 agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat
    27961 ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc
    28021 tgtactgcgt tctcccatct caacttctcg cttattagga gaattatcca gttatttaga
    28081 tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg
    28141 tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa
    28201 acgcggtcat cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac
    28261 ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat
    28321 cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt
    28381 ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga
    28441 cacgaccgtt gaaatcgcgg aagtaggcat tccacaaata aaaatccctg tcaaaacagg
    28501 acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg
    28561 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
    28621 caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
    28681 gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgccatg
    28741 tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt
    28801 atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat
    28861 cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct
    28921 atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt
    28981 atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc acaagcaatc
    29041 ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc
    29101 ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat
    29161 cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg
    29221 tttttacgaa agaaaccaca ccttttgaaa aaaggtgaag tccttttatg ttatctaatg
    29281 tggtatagtt tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta
    29341 ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta
    29401 tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat
    29461 caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
    29521 gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct
    29581 cgccgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
    29641 caacacccaa cacacgaatc aacgttattt accagatcat tgtctacccg attccatcca
    29701 agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc
    29761 aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt
    29821 gatcatccat gcaagtaaag gattagaaca ggggacacat aaaaggatct ctgatatctt
    29881 gttagaggaa atcccagtag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca
    29941 tgcagaagaa gtagcgaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa
    30001 agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc
    30061 tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg
    30121 cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt
    30181 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt
    30241 aagcggagta ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc
    30301 aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt
    30361 cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaataa tggaaatcga
    30421 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
    30481 cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt
    30541 ttgaaggagc gaatgtatat gaaagtcaaa aaagcagtta ttccagcagc aggattagga
    30601 acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa
    30661 ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc
    30721 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa
    30781 acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat
    30841 gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa
    30901 gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa
    30961 gacgaagtac cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg
    31021 atcgccgtta tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga
    31081 gaagtattgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
    31141 aaagcaccaa gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat
    31201 gtattgtccg agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat
    31261 acattgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc
    31321 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta
    31381 ggtgcacctt tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc
    31441 aagatcgatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc
    31501 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
    31561 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
    31621 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
    31681 ttttatcgtt cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt
    31741 agcagaagca aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca
    31801 agtagaaggc ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc
    31861 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa
    31921 tcattctagt aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc
    31981 gacggttgcc ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac
    32041 tgaaacgaca ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta
    32101 gtcggaggca cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa
    32161 cttcgggatg aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca
    32221 gatatggcga aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat
    32281 ctttctcaac aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca
    32341 gatgaagcag gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac
    32401 ttatctaatc gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga
    32461 agtattgcta aagatacttc agaagatatc atgattgatg taaaagacgc tgctggtaaa
    32521 gcaggagata ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa
    32581 gaaatgatga aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca
    32641 atgacagatt catctacaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc
    32701 ggactagctc tcaccttttt tactcttatt tttctaaaac aagtaattct tttggtgtag
    32761 tcgtgaatgg cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta
    32821 ctttttcagg aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta
    32881 agtcatttcc ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta
    32941 aacggtgatt gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat
    33001 caagttcacc tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa
    33061 gagtgatgtt gtagattttt tcttggaagt cggttggttt ttgataagca attgtacgag
    33121 tagcatcact gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg
    33181 gtgaaatctt cgtatttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga
    33241 ctaatgtgtc gaaagacatt tgtgaaacac cttgtttttt caattggtat tcaatttctg
    33301 caatgatcgc ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa
    33361 catcggccca tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt
    33421 gtagacgttg gatgatggaa gttagatcag tgtaagaatc cgttcccgtc aagatatcag
    33481 agatagcatc gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct
    33541 gaccatagcg agcacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat
    33601 agccacggac atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag
    33661 tgaaaaggaa aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat
    33721 cactttcgta tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga
    33781 cttggtgttg tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac
    33841 ctactttcta aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac
    33901 gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt
    33961 cttgagatta tttgatattc tattttagaa atgtaagacg tattttcaga aaacatataa
    34021 aatatgaata ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga
    34081 gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgttaaa
    34141 ccagcaacaa gaaaaaaagt cttggaagtt atcgatcgct tggattatcg tccaaacgca
    34201 gttgcgcgtg gcttggcaag taagaaaaca acaacagttg gtgtaatcat ccctgatgtc
    34261 agcaatatgt tctttgcttc tttagcacgt ggtatcgatg atgtggcaac aatgtacaaa
    34321 tacaatatta tcttagcaaa ttcagatggc aacgaccaaa aagaagtcaa tgtattgaat
    34381 aacttactgg ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat cacagacgaa
    34441 atccgtggag aattttcacg ttcaaaaact cctgttgtat tagctggctc tattgatcca
    34501 gatgaacaag taggtagcgt aaacattgat tatgtatccg caacaaaaga tgcagtaaac
    34561 aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt gatcgatccc
    34621 atcaatggac aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa taacctacca
    34681 tatagcgaag gattgatctt tgaagcacaa tacaacttca aagatggtct atctctagtc
    34741 gatcggatcc ataatagcgg tgcaacagct gtctatgttt cagatgatga attagccatc
    34801 ggtattttgg atggtttatt agaccgtggc gtgaaagtac cggaagaatt cgaaatcatc
    34861 acaagtaaca attcactact gacagaagta gctcgtcctc gtttaacaag tatcacacaa
    34921 ccattatacg atttaggggc tgtatctatg cgcctattga cgaaaatgat gaataaagaa
    34981 gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atctacaaaa
    35041 taataaaagg acaaccattc agaggttgtg tgcctgccgt ttaactctgc gttacaagga
    35101 gcaatttcaa aaaacagcat ctcatgtttg atgaatattg tgactcgtga ttcggagttg
    35161 gcaggagaac accgtttatt tgggaaaagg ggttatgaca aaattttgtt ataatccttt
    35221 ttttgtgtct taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat
    35281 ccgtgtatag atgctgcatt ttgttcacca ccatcaaaaa aataagggac tgtgacaata
    35341 ctattgtcac aatcccttat tactgtgtat tctattaaat caatcatctt tctcatctga
    35401 ttctttagtt gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata
    35461 gttttcatcg gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc
    35521 ccatagcgat tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt
    35581 tccaggtgtt tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc
    35641 gaatttctca tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt
    35701 ggcaagataa ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg
    35761 ccctgcccag ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta
    35821 ttcattggta gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct
    35881 tgagatggca tctttgaatg gtgtagtgat ttttgcattg atcacactac gcatcatgtc
    35941 attcatgatc gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata
    36001 aattacattt cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc
    36061 gccgtttgct aaagcctgaa atccgttggt ctgttgaagc gtcgtgacat ctactgtacc
    36121 taaaggagca gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta
    36181 gtgctcgtaa gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata
    36241 agccgggata ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc
    36301 ttcgttagta gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc
    36361 ataatctgat acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac
    36421 aagaactggt ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg
    36481 attctgactg taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat
    36541 caatacattt ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt
    36601 ttcaacgcca ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata
    36661 tcctttattt tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc
    36721 agacatatca gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt
    36781 catcacagta taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa
    36841 gtcttttgta aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc
    36901 acgatacata ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt
    36961 tagcgcgcct gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat
    37021 ataagcagct tgcggcagcg tcacatcttt tgcatctaca ccaaagattc cttttgcagc
    37081 ttcctggact ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa
    37141 atacgtagca atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat
    37201 ctcgtttgct ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg
    37261 ctgtgtcaaa gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg
    37321 taaaacagct ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat
    37381 aattgcagtt ttcaataaag gtgaaatctt atcagatgaa atagtcgtgc gcatcagatc
    37441 tgtctggatt tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat
    37501 gtttcctagt tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata
    37561 ggcaaaatag ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt
    37621 aataaaaaca gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc
    37681 tttttttctt ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac
    37741 taatggtttt atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt
    37801 tttggcagga atttcttttt taaaagagtt aaaacgtgag aaaatgatct ttattataaa
    37861 agagttctta agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa
    37921 aaatcaatca ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt
    37981 gcctttcaag gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga
    38041 aaatgctgtt aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct
    38101 atacaggcgc ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg
    38161 gaaaagcgta catggcagaa aaaggtctaa caaatatcgt cgttcatgcc ccttatatca
    38221 ttaatttggg gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag
    38281 aagagattgt tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc
    38341 atgtaggggc aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt
    38401 tgacaaaaga acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa
    38461 tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat
    38521 tatccgttac ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact
    38581 tttcaggtgt aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc
    38641 atatcaatga ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt
    38701 ttggaacaat cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata
    38761 tttcgaaaat cttggaaact ccctatgtag gcaaagataa aaagaaagct tacgcaccat
    38821 atggaaaaga aatcgccatg ttcaaagcgc aaacattcga tccagaagtg tttcctgagt
    38881 tattagcaga agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag
    38941 gcacattgcc ttgtcctttt ttgtttaaga aacaaaggag tactttttgc agtctttttc
    39001 atcatgcatc ttctcccccc tctttttaaa tttagacaaa caaggtatac tggtagaaaa
    39061 gtaaaaaaac gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat
    39121 gctgaacact ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct
    39181 tggcatagcc ctattattta caattattcg tttgtattta tatagtaaag caatctatgg
    39241 aacagacatg tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt
    39301 ggacggtaaa tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag
    39361 cttattttta gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt
    39421 cagtattttt gccagtatat tggttgcact cgcgtttaaa ccgattgtga agaacaagta
    39481 tcttctttta agtatctatt tttatttttt gttttctccg attagtttta cacatgaata
    39541 ttccactcga acatatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg
    39601 tttattagct atttattacc gcaaatacgg ttctagcaga aaattgctgc catggttttt
    39661 agggctttct gtcgcttttc ctttcttttg gttcattcgc gaagattcta tctggcttct
    39721 tccttttact gtagtagctt tgggtatcag tttcgtccag atcttgctag ggcaagactt
    39781 ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt cttctctcga aacgggaatg
    39841 gctgaaactt tcattgtttt tacttcctat agttggcttt tccatcgtct atcaatggat
    39901 cgaatataaa aatgagacaa catacggtat tgcaaccatc aatgaccgtt ccagtggaga
    39961 atttggacgt ttgatgaaac aactgatccg aatagatgat gaaacgaact tgaataaaga
    40021 aaacagtgag atatgggtct cacatgctgc actaaataaa gcattagccg catctcctac
    40081 attccatact gtttctaccc gtattgacga attatatacg actcacgcgt ggactcaagg
    40141 tggaaaagta aaagaattac ccggagatat catcttttgg gcattgcgag atgtggtaag
    40201 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    40261 ccaagaatta gaagaagcct atcacaaggg aacaatcaaa aagaaaaaag agttctatct
    40321 gatgggatca ggagatggaa agacgatgag tgatctgcca atcgtattcg atttccttca
    40381 atcaggatac gcttatgcat ggcgatataa agatttccat caaggaggaa attatagttt
    40441 cggaacgcaa gaacaagtca aacaggctca agaattatta aaccttccgc taatggagaa
    40501 ttggatcgat agtaatgaac cgagaacacc taatttccaa ttaacaaaat cagcaaaagt
    40561 cgcgaatgtc atcataaaaa tttaccaagt tctggctatt cccgtgctga ttgcttcaat
    40621 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    40681 tagttcctct ttgattgtgg tcaccggatt aatactgaca caattggttt ttcttttcgg
    40741 tgtctcatgg ttttgttctt atgcgccaga aaaaaaggac tattttttaa gtgtctacac
    40801 tggtgcaggc gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa
    40861 attaaatctg aaaaaagctt tatcttctaa ataaaaagac ccttggtagc tagattttta
    40921 acctaactat caagggtctt tgtgtggaaa aatcagtttt tattcgattt cttctaccgt
    40981 ttcctgcaaa agctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc
    41041 caacaggaga aggacaagaa caatgattac ataagcgatg tgatcagcca atgctagttg
    41101 agcggaagtg gcttgctcgc cgaagaaact ttcgatctgt cctggtaatc ctcgtaagtt
    41161 caagtaagtt agactaatca cagagaacca gccaagaagt tttacgatga atgaattttt
    41221 aaatcggcta cccatctccg cttggctgtt ggtcatcatc agtaaaggaa tcatcgaaaa
    41281 cggcaacgca aaagccaaga acacctggga ttcattcatt aagttattca aagcggcatg
    41341 ttcttcgatc gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga
    41401 aagcaatcgt gtgaccagtc ttcttagcca aatcgggata cgcatatgga tgaatccttc
    41461 catgatgact tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag
    41521 tgctacagca aataaagtgg acaagatacc agattttgct acagcaatca aaataccatt
    41581 gctcatagct gatggatcag ataaagcttc atacagacca aaaaaggaag gatcttggac
    41641 agaaccggtt ttgaagacag cgacacccat gatcaatagc agtgcattga ctagaaaagc
    41701 cattgacaac tggatattag aatcccaagt agaaaaacgt acagcttgtg ctacatcttc
    41761 ttcgttttgg tgatcgatct tccgagtttg tgaaactgct gaatgaagat ataagttatg
    41821 cggcatgact gttgcaccaa taatccctaa agctccagtc agcggagttt cgccgccgat
    41881 gaccggtgag tctgagaatg tatgactgtt agggataaag ccagcaaata aactgtgcca
    41941 atctgggtta gacaagccaa cttgatagac aaaaacgaat aaaatgacga aaattaagca
    42001 gacaaccagt gcttcgatct tacgaaaccc gacttttgtc agtagtaaga gaacgaaaac
    42061 atctaataca gtaataaaga cggcaatcac caatggaata tcaaaaagta agtagagggc
    42121 gattgctgca ccaatgactt ctgcaatatc tgttgccata atggctaatt cagtcaagat
    42181 ccataaaatg acacctaatg tcttacttgt gcgtgctcgg attgcctgtg ccaaatccat
    42241 ttggctcacg attcctaatt ttgctgccat atactgtaaa agcatagcaa tcagactaga
    42301 catcaaaata actgacatca ataaatattg gaaattttgt cctccggtga tcgaagtaga
    42361 ccagttaccc gggtccatat acccaactgc tactaacgca ccaggtccag agtaagcaag
    42421 aagtgtttta aaaaaacttt tcccctttgg tacatcaact gttccattta cttcagctaa
    42481 agataaacca tttgtatgtt caataagtcg atgcttatga aattgtttat tggattgatt
    42541 attcaagaaa aaaccacctt tcctatttat ctacgattac attctacacc tcaaaaagaa
    42601 aaaattaaag aaaaagattc ggtacgccta attttttttc atggcgcgtc ttgctagatg
    42661 acgaaagaaa ttttgttctc ctctaaaaaa accactattt tttgcatgaa actaaagaat
    42721 acgcctttct tttttcaata aactttagta aaataagtca ttgagagcaa ttcggaaata
    42781 gaaaggagag atttatatgt tatcaacagg tattgtcgta cttattgcta ttattgcttt
    42841 gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga
    42901 aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa
    42961 accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata
    43021 ggttctgacc caacgacttt ttacgggctg attttttcag tccgtttttt atttcgtaat
    43081 ttgttagact attcagatta ttcagcaaaa aagacaagca cagacaatcc taggctcttg
    43141 ataaagacaa aggaggagta aaatgtcgat ttttagaaaa ttaggctggt tttttcgtca
    43201 ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt
    43261 agttcctcca aaagtgattg ggatcatcat tgatgagatc gccgataaaa atttatcaat
    43321 aaagatcatt ttcgggtgga tcttcctatt gattatcgct gcttttttac aatatctttt
    43381 tcgttatatc tggcggacga atatttgggg aagtgcagct cgattggaga aagaacttcg
    43441 gcagcagtta ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa aatatcggac
    43501 tggggatttg atggctcacg caacgaatga tttgaatgcg atccagaatg tagcaggagc
    43561 tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat
    43621 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt
    43681 cacttcacga gtgctggggt caaaactcca tgatgctttt cgtgattcgc aagaagcttt
    43741 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    43801 tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa
    43861 aaacaaacga gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg
    43921 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    43981 gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    44041 gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga
    44101 acttttgcat gaaaagacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa
    44161 aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt
    44221 agaacaaatc aagtttgcga tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    44281 tgcgggaaaa acgacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    44341 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    44401 cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt
    44461 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    44521 caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc
    44581 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    44641 attattaata ttagatgatg cactctctgc agtagatgcc aaaacagaag aagctatctt
    44701 gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag
    44761 tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    44821 aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca
    44881 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    44941 acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc
    45001 aaacctttta gaagaacgtt catcgttgct atcttatttg cgtttgcttt gtctgtgatc
    45061 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg
    45121 gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt catagtaaaa
    45181 agtatcattt ggtttttcca atggtatttg tattccatgg cctcattgaa gacctatcag
    45241 tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag
    45301 acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    45361 tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc attccttgcc
    45421 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgtttttgcc gattttagga
    45481 atcgtcatct ggtattacca gaaattcagt tctcgtattt accgaaatat gcgggaaaaa
    45541 ttaagtcagc tgaacacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    45601 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac
    45661 acgcgttatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta
    45721 tatgcattgg cgattgcgtt ggtgttgact ttgtttggga tcgatgcgtt gcattctccc
    45781 gtagaagttg gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg
    45841 tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc
    45901 ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg
    45961 attacttcag gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    46021 gtattaaaca atatctcttt tgttgctaat ccgggagaga ccgttgcatt agtgggacac
    46081 accggaagcg gaaaaagctc gatcatcaac gtattgatgc ggttttacga attttatgaa
    46141 ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    46201 aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt
    46261 cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa
    46321 gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt
    46381 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat
    46441 cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg
    46501 atccaagaag gattggcaaa aatgcgtcaa ggacggacaa cgattgcgat tgcccatcgg
    46561 ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa
    46621 cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    46681 caaagcagtg gtatataaaa gatgttgtga cagtagaatt ttgacctaag cactagagtg
    46741 aaaataaaac atagcttctc atatttttca tctgtgagag ttagtgtcgt agtcaccaga
    46801 tttaaaaaaa gattcgacag ctagtcagaa gtgcctaata gccactaaga aagcgttgac
    46861 aaaagcattt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    46921 tgcaagctaa acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt
    46981 ttgttttgga tacagcccta tacatcatgg aaaggagcaa aacatgctga ttgagaaagt
    47041 actgaataac aacgtcgtaa taaccaggga tgaaagaaat caggaaatga tcgtgatggg
    47101 aaaaggtttg gcattcaaaa agaaagtggg tgacccgatt tttgactcat gcatcgacaa
    47161 aaaatttcag ttgacaaatg acgggctttc acaacagttc caagaattgg cacaagcgat
    47221 tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctaca
    47281 agtccagttg aatgattctg tgtatattgc attgacggat catttgtata cggctatcga
    47341 acgtcacaaa atgggaatat ttgtaccaaa tgtattgctg tatgatatca aacaactatt
    47401 tcctaaagag tatgccatcg ggaaaaaaac agtgaagaaa atctcggata aataccacat
    47461 caagttatca gaagatgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat
    47521 tcaaagccag caagacaata cctacctgtt tactataaca atacaggata ttctaaatat
    47581 cgtccgctat tactataaga ttacatttga tgaagattca gtccattttt ttcgattcgt
    47641 cacacactta cgtttttttc tgacacgcgt cctagatgga gaaaaatcag aagtgggaga
    47701 agtttctgag gaattgtttc aggttattca aaaaaaatat aaggatacgt ttggttgcgt
    47761 tgagaaaata gcagactttt tagccgagac gttcaactac cagatgtcca cggacgaaaa
    47821 aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaaaaaa
    47881 ggaggataaa ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct
    47941 tccactaata aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc
    48001 gtagaaaatg tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt
    48061 cgtttcaaat taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt
    48121 attgtaacgg taatgcgagc gggagggcag tatcaagttg tcattgggaa tcatgtgcca
    48181 gtcgtgtttg aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca
    48241 gaaaaaatga gaccgtttga tatgcttatt gatggaatat cgggctgttt ccagccattc
    48301 cttgctatcc tagctgctgg cgggatgatt cgtggactga cagcattcct tgtcttctta
    48361 ggcgcgtttg accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta
    48421 ttccaattta tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa
    48481 ttcgtaggga tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg
    48541 agtggtgcag cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct
    48601 atttatcaaa cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt
    48661 cccattatct ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta
    48721 cctgagatga tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta
    48781 ggttttttac tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt
    48841 gaaacacttt tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa
    48901 attctggtga tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct
    48961 gtgaatggtt ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca
    49021 gcacttaccg cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca
    49081 gccattgttt ctggtattgt ggggattaca gagccagcta tttatgggtt tacattacca
    49141 cgtaaaaaaa tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg
    49201 ttaatgaatt taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat
    49261 attagaccag atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg
    49321 atggtggtat cctttacgct gacttttttc ttttggaagg atgaagcagg tgaaacagat
    49381 gatatacaga aaaaatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct
    49441 ccactaaatg cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg
    49501 atctaccctg aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gaccctgttc
    49561 ccgacgaaac aggcaattgg aatggtttct gaaacagggc tggaattgct gattcatgtc
    49621 ggattggata ctgtgcagtt ggaagggaaa tattttgaaa gcttagtgca gcaaggataa
    49681 agagtgaaac aaggcgatct gttgctacgc ttcgacaaaa agaaaatcga aaaggaagga
    49741 tacagtttag aaacacctgt gatcgtcacc aattacacgg attatttgga tattttggaa
    49801 aaccaagggg agacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt
    49861 aggtgaagaa tggactaggc ttaactattc tttttattcc acttctttat tttcaaaatt
    49921 gaaaggctct ttcatgatgg ctgagctgat agggcgttta tcgaattttt gaccgacaga
    49981 aattagatgg actaaattct gttgataatt ttctgcaaaa acacaactat ataacacgtc
    50041 tagaagataa caaattgatg tattgatcgt aaaattacca atttttgaat acagtcgctc
    50101 gcgtgtcgta agtcgcaaag cggctgtact cattgcagat agcgttgaat caccgatact
    50161 ggtcaaagct aaaattggaa catgattttg tttcaatatt tctgcggtac gaaggatcac
    50221 attgttttca cccgtataag aaatcagaat cccgcacata tcactcgtgc aattgtacgc
    50281 ttcatagaat gattcaccaa aagtggtgac aatcgtgaca tccattttga tgcgcttcat
    50341 tttcagcatg aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt
    50401 ttttgcttga agcattagtt gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag
    50461 tgtatcatga atcgtcatct gttctaaaga agccatttta tttgcgatcg tcataatacc
    50521 gtctgtggtg gaaaaaggaa gattcgcatc tacgtcttga aaattccctt caagataggc
    50581 tgtttcagct aaaaaagcat tacgaaaact gacccagcca tcaaaaccca ttttttttgc
    50641 tacacggatg agtgtagagg ggtggacata acatcgctca gcaatcgctt ggatggtcat
    50701 ttcgccaagt gctggagaat cgcttaagag aaactggatc acttcatttt ctgaaggaga
    50761 aaaggagatt tctttgattt tttcacgtaa taacatgtag ataccttctt cttatttttt
    50821 tacgtaaatt ataacttttt tgaaaatgat ttgtaaacgg atttatacta tataacttaa
    50881 aatagacatt ttttgacaag atatttacaa aaatccacta gaaaacagta aattttgtaa
    50941 atgttactgg aaagaaacag atttctattg tttgttttgt ttacaaaaaa gataatgaaa
    51001 gtgtaaaaaa gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg
    51061 gaggcagcag ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac
    51121 cgatacgaga aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtta
    51181 gtcagatgtc tcagcgtatg tgggatgctt ctccgtatga agtcaagatc tatgcaactc
    51241 tggatcggga agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc
    51301 aattagatgc acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag
    51361 aaacaaatgg tgctggcggg atattcaaag cttttcgtac cgttccagtc gttttggcaa
    51421 tcgttgagga catgaagcgt ttatgtccaa atgcttggct gatcaatttc gcgaacccaa
    51481 gtgggatggt gacagaagca gttatcagat atggcaaatg ggataaagtg attggattat
    51541 gcaatgtgcc agtgatggcg atgatgacag aaccagcaat gcttggcaag aaaaaagaag
    51601 agctgatcta caagtttgcc ggtctaaatc atttccattg gcacaaagta gcagataata
    51661 aaggaaatga ccgcacggca gaattgattg atcgtttata cgatgaagag aatggattac
    51721 cgaaaaatat ctttgatatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc
    51781 cttgtggcta ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag
    51841 aatatcaaac gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctatttg
    51901 aattatacaa agatccagaa ttagaccata aacctgaaca attgcaaaaa cgaggtggcg
    51961 cttattattc agacgctgcc tgtgaaacga ttgcttcaat ctatggaaat aaagaaacac
    52021 agatcgtggt atctacaaag aatgatggag ctgttcctga cctgccggca gattgtgtag
    52081 tagaagtcac tgcctatgtt ggcgcacaag gagcgagaaa tgtagcgttt ggttctcttc
    52141 ctgctgctga aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag
    52201 cagctgtgac tggtgattat gggacagctt tacaggcgtt tacgatcaat ccacttatac
    52261 gatcaggaaa gacagcaaaa cggatcatgg atgagttgtt tattgcacac cagcagtatt
    52321 taccgcaatt caaggagaca atcagccgat taaaagaaga aggaatcgaa gttcaagatg
    52381 aactggcaag agaattatca ggtaatacac atataatgga ggaagtataa tgggattcag
    52441 aaaagatttt ttatggggcg gtgctacagc agccaatcaa tgtgagggcg gttatgatga
    52501 aggtggacgc ggactagcaa acgtagatgt tgtgccaatc ggaaaagacc gttttcctgt
    52561 tatcaccgga aagaaaaaaa tgtttgaatt tgatgaggat catttttatc cagcaaaaat
    52621 tgcaatcgat atgtatcatc gttataaaga agatattgct ttgtttgcag aaatggggtt
    52681 caagacttat cgaatgtcca tcgcctggag tcgtattttt ccaatggggg atgagaaaga
    52741 gccgaatgaa gctggcttga aattttatga ggatgccttt aaagaatgcc aaaagcacgg
    52801 aattgagcca cttgtgacga ttacgcattt tgattgtcca atgcatttga tcaaagaata
    52861 tggtgcgtgg cgttccagag aactcgttgg tttttatgaa aatctgtgtc gagtcatctt
    52921 caatcgctat aaaggacttg tcaaatattg gctgacattc aatgagatca atatgatcct
    52981 tcatgcacca ttcatgggcg cagggctgta ttttgaaaaa ggagaaaatg aagaacaaat
    53041 caaatatcaa gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agattgctca
    53101 tgaagtagat ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata
    53161 cacttgcaat cctgaagatg tttgggcttc tagaaaatca gatcgagaaa attatttctt
    53221 cattgatgtc cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggagcgaaa
    53281 tggcattcag atcgaaatgg aaccagggga tgccgcattg ctgaaagaac atactgttga
    53341 ttttatctct ttttcttatt atgcttcccg agtttcgact accgacaacg aactcttgga
    53401 acagactgca ggaaatatct ttgcttctgt caaaaatcca tatctgcaag caagtgaatg
    53461 ggggtggcaa attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta
    53521 tcaaaaacca ttgtttattg tagaaaacgg cttaggtgca gtcgatacgc cagatgaaaa
    53581 tggttatgtg gaagatgatt accgaatcga ttacctggca gcgcatatcc aggcgatgaa
    53641 ggatgcagta gaactagacg gagtcgatct attaggctat acgacttggg gatgtattga
    53701 tttagtatca gctggtaccg gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg
    53761 tgataaccaa ggaaatggca cattagcccg ttcaaagaaa aaatcattca actggtataa
    53821 aaaagtaatt gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag
    53881 tctattgttt ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga
    53941 acaatagact ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa
    54001 tctacatatg tatgatgtaa tcgttattgg cgcaggtcct gctgggatga ctgctgcttt
    54061 atatgcttca cgttcaaact tatccgtatt gatgatcgaa caaggtgccc ctggcggcca
    54121 aatgaacaat acggcagaaa tcgaaaatta tcctggattc gattcgatca tgggccccga
    54181 tctagcttat aagatgtatg aaagtgtctc aagatttggg acagaaaatg cttatggaat
    54241 cgttcaagat atcaaggatc acggcagcca taaagaagtc atctgtgaag ataaaagtta
    54301 cgaaggaaaa acagtcatta tcgcaacagg atgtatccac cgtaaattag gcgtacctgg
    54361 cgaggaagaa tttgctggac gaggcgtttc ttactgtgca gtctgtgatg gtgcgttttt
    54421 ccgtaacaaa cgtctagtag tcatcggggg tggggactca gcagtagaag aagccatcta
    54481 tttgacacaa tttgcttctg aagtagtgat cgtccaccgt agagatgaat tacgtgcaca
    54541 aaagatcatc caagaccgtg catttgcaaa tgaaaaaatt tcctttatgt gggatacagt
    54601 cgttgacgaa atcgttggaa acgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa
    54661 aaccggcaat gaatttgatt tagcagcaga cggagtattc atctatgttg gactggatcc
    54721 attaactgca ccttttagag aagcaggttt gacgaacgaa gctggctgga ttccaacaga
    54781 caacgaaatg aaaacgaata tgccaggcgt ttttgccatc ggagatgttc gtgaaaaaga
    54841 tttgcgacaa atcactacag ctgttggtga cggcggtatt gctggacaac aagtgtataa
    54901 atacttggag tctttagaag aaacaacagc ataagtttaa aaagagtaga agaacgggtt
    54961 caattgttct tttactcttt tttttcgcgt gaaatttact gaatctgatt tatgcgaaca
    55021 cgtccaacac cttttgataa aagaatcctt tcttgattta agcgtttttt ggtcctcata
    55081 ctttcatatc taataagtga atgctatact tattaagaac catatttcag aaagaggtgt
    55141 tattttcatg tcttgggaac aagtgtatga acaatgggta catgaagaaa atttagaaga
    55201 aaacttaaaa aaacaattaa atgatctggg tgaagatcct gaaaaacttg aagatgcttt
    55261 ttatgcaccg ttggaattcg gtactgcagg tatgcgggga atcttaggac ctgggatcaa
    55321 tcgaatgaac atctatacgg taagacaagc aacagaagga ctagcacgtt tcatggatac
    55381 acaaggtgaa gaaacaaaac gccgaggtgt cgctatcgct tatgactcac gccatcagtc
    55441 accagaattt gcaatggaag cagccaaaac actagcacat cacaatatcc cgtcttatgt
    55501 attcgaaagt ctgcgtccaa caccagagct ttcttttgca gttcgtcatt tgaagacgtt
    55561 tgcgggaatc atggtaaccg catctcacaa cccagcggct tacaatggat ataaagtcta
    55621 cggagaagat ggcggacaaa tgcctcctgc agacgcagat gcattgacga aatatgtccg
    55681 tgaagtcagc aatccgctaa aagtcgaagt attatctgac gaagaagcta aacatagcgg
    55741 gttgatcacg atcatcggag aagaagtgga cgctgcttat ctagaagaaa tcaaagcagt
    55801 gacgattgat cgtgaattgg ttgaaacaat ggggaaagat ttgaaacttg tgtacacgcc
    55861 acttcatgga acagggaaaa tgttgggcga acgtgcgttg aaacaagctg gattcgaaca
    55921 attcgtacta gttcccgaac aagctgtggc agatccagat ttcactacgg tcaaatcgcc
    55981 aaatccagaa gaacattcag cttttgaata tgctatacga cttggagaaa aagaaggagc
    56041 tgacttgctg attgcaacag atcctgatgc agaccgttta ggcgcagcgg ttcgtttgcc
    56101 agatggttct tatgaagtat tgactgggaa ccaaatcgga gcattgtttg ctcaatatat
    56161 tttggaagca catcaatcaa gaggcacttt accagaaaac gcagcggtat tgaaatcaat
    56221 cgtttccagc gaattgccta cagcaattgc ggaacactac ggtgctacca tgttcaatgt
    56281 gttgacaggg tttaaattca ttgctgaaaa aatccaacaa tttgaagaag accattcaca
    56341 tacttttatg tttggattcg aagaaagtta tggttattta gttaaacctt ttgttcgcga
    56401 taaagacgcg atccaagcat tgcttttatt agcagaagta gcagcttact ataaaaaaca
    56461 aggcaaaacg ttgtatgacg gcttgcaaac gatttttgaa acctatggct atttcgcaga
    56521 aaaaacgatt tctgtgacaa tgagcggaca agaaggttct gcgaaaatct ctgctttgat
    56581 ggaaaaattc cgtaaagaag caccgacaga atttgccgga gcaaaagtta ttcaaacaga
    56641 agactttaaa gaattgacga gaacagaccg tgaaggaaac gtagaaaaac taacaacacc
    56701 gccatccgac gtattgaagt atacattgga agaaaatggc tggattgcta tccgcccttc
    56761 aggaactgag ccgaaaatca agttttatat tggtgtaaaa ggaagttcta cagaagacgc
    56821 agccgaaaaa gtagcctctt atgaagcgat tatcaaaaag atgacagaag aataaaaaac
    56881 ttgagactga tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg
    56941 aatggattgc cagacacgca aaaatcaaaa gtagagaaag tcagtcaatt attcaaaatg
    57001 ttgagtgatc ccactcgtct taaaattctg ctatatctga aagacgggga acaaaatgtg
    57061 acagcaatca cccaagcagt ggaaatggag cagtctgctg tatcgcatca actacgttta
    57121 ttaagagaaa atcatgtcgt caagtctcat cgcgagggaa aagcgatttt gtatagtctt
    57181 gatgattctc atgtactgga tattttgaac cagacactca aacatgtgga acatcaataa
    57241 cgataggtgc aaatagataa acactggagt gtgacaacga agaaatttcg ttgtcacact
    57301 ccagtgtttt ttaagaaaaa gacaaaaatg atcaatcgct tttttctgtt gtttcagttc
    57361 cataataact atgatcttct aggtctaaag cattcaaaat aagcgaagcc gtttcttcaa
    57421 tagataaaga tgctacattg atcactacac atcctagttt ttcataaaga tcattcgcaa
    57481 aatcgagttc agctctaatt ttttcgatat cggaataagc agtatcaggg tttaatccgt
    57541 aagagcgcat ccgttcttta cggatattat ttaaaatatc tgggttattt gttagtccca
    57601 cgattttttt aggatcgatc tcccaaagct gtttgggaat atgtgcttgt gggatcaaag
    57661 gtaaattggc aacttttaaa tttttgttag ctaaaaacaa gctcaaaggc gttttggaag
    57721 ttcgggatac gcctagcaaa acgacatctg cttctaaaaa accacgagga tctttcccat
    57781 catcgtattt tacagcaaat tccattgctt tgatccgttt gaaatagttc tcattcaata
    57841 aatggagggc accaggttgc cgaattggct tgataccagt taaacgttca atttctgata
    57901 tcggtggcgt catcacatca aaactaaata attgatgttc ttcacaaaaa gaacgtgtta
    57961 tctcgatcag ttcttgattg attaatgtat gcaggacaag tgctttttct tctaatgcat
    58021 cttgcaatgc ctgccttaat gttttttcat ctgtcacgaa cgttctgcgg aataggttga
    58081 actcaacggt aggatactga gccatagtag cttgcgctag ttttgtcgca gtttctccag
    58141 cagagtcgga gatgacaaaa aaagtaacga tttctgtaga atcggaagtc atggataaaa
    58201 cctcacttgc atttttttta tgtttattat accatactct agaatgcgta cttcgtaatc
    58261 attttgattt ggagggataa gtcatgaaaa aacagacatt gatcgaacaa tcattagaaa
    58321 cattgaaaaa taacggtttt aaatatacta aaaaaagaga agcattgtta acttatttag
    58381 tgaagcgtaa ccgctacgta tctgctaaag aagtttttga ctatatgaat caggaattca
    58441 aaggggtaag ttatgatacg atttaccgga atctccatga ttttgcagag atcgatttac
    58501 tggaagaaac cgatttaaat ggagaaatga agtttcgttt tcattgctgt caggggaaaa
    58561 tcggccatca ccatcatttt atctgtacag tgtgcggaac aacgaaagaa atccatatgt
    58621 gtccgatgaa ttatttcgaa gaacagctag gtggatgcac gatcgaaggg catagattcg
    58681 agattttcgg aagatgtgaa aattgtcaat aaggatttaa ataaaataac atttgataat
    58741 tttaaaaaaa agcaagattt ccttgacggt caaaacagca ttagatataa tatttacagg
    58801 acagtcttgt tttttgagat atatttcaat aacaattacg gttacttttt aagctcggag
    58861 ggagggaatt aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct
    58921 tcgtcgcttc aaacgttccg tgtcaaaagc gggtacttta caagaatctc gtaaacgtga
    58981 attctacgaa aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa
    59041 aaaattctag ttctgtgatg aattggagtt ggtagtgtgt cactactaag tagattaaac
    59101 gatgacatga aagcagcgat gaaagcgaaa gataaggaat ctttacaagt catccgaatg
    59161 atcaagtcat ctatacaaaa cgagcagatt aaagaaggtc acgacttgac agaagaagaa
    59221 gaactaacgg tgttgtctcg tgaaatgaaa cagcgacgtg attctttgca tgagtttgaa
    59281 gaagcaggac gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat agtcgaaaaa
    59341 tatatgccgg aacaactttc cgacgaagaa attcgacaac ttgttcaaga agcaatcact
    59401 caaactggtg cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat tatgcctaaa
    59461 gtcaaaggaa aagcagatgg aaaccaagtg aatgcgattg tgaaagaact attacaatct
    59521 taattttatc atttaaactg cggacagtga ttatccgcag ttttttgtgt tcgtaaaaaa
    59581 agaactaaaa aacagctaga agaaagaatc ttattctt
//
LOCUS       DADXTH010000007        56383 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.007, whole genome shotgun sequence.
ACCESSION   DADXTH010000007 DADXTH010000000
VERSION     DADXTH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.007"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            326..1171
                     /locus_tag="IWI97_000417"
     CDS             326..1171
                     /locus_tag="IWI97_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2461950.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1452..2672
                     /locus_tag="IWI97_000418"
     CDS             1452..2672
                     /locus_tag="IWI97_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBC2461951.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2669..5389
                     /locus_tag="IWI97_000419"
     CDS             2669..5389
                     /locus_tag="IWI97_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2461952.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5373..6317
                     /locus_tag="IWI97_000420"
     CDS             5373..6317
                     /locus_tag="IWI97_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2461953.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6386..7396)
                     /locus_tag="IWI97_000421"
     CDS             complement(6386..7396)
                     /locus_tag="IWI97_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBC2461954.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7576..7920)
                     /locus_tag="IWI97_000422"
     CDS             complement(7576..7920)
                     /locus_tag="IWI97_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBC2461955.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7913..8347)
                     /locus_tag="IWI97_000423"
     CDS             complement(7913..8347)
                     /locus_tag="IWI97_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBC2461956.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8485..9222
                     /locus_tag="IWI97_000424"
     CDS             8485..9222
                     /locus_tag="IWI97_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2461957.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9215..10426
                     /locus_tag="IWI97_000425"
     CDS             9215..10426
                     /locus_tag="IWI97_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2461958.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10630..11412
                     /locus_tag="IWI97_000426"
     CDS             10630..11412
                     /locus_tag="IWI97_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBC2461959.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11482..12144
                     /gene="trmB"
                     /locus_tag="IWI97_000427"
     CDS             11482..12144
                     /gene="trmB"
                     /locus_tag="IWI97_000427"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBC2461960.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12242..13996)
                     /gene="ade"
                     /locus_tag="IWI97_000428"
     CDS             complement(12242..13996)
                     /gene="ade"
                     /locus_tag="IWI97_000428"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBC2461961.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14128..15057
                     /gene="tadA"
                     /locus_tag="IWI97_000429"
     CDS             14128..15057
                     /gene="tadA"
                     /locus_tag="IWI97_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBC2461962.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15014..16063
                     /locus_tag="IWI97_000430"
     CDS             15014..16063
                     /locus_tag="IWI97_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBC2461963.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16060..16338
                     /locus_tag="IWI97_000431"
     CDS             16060..16338
                     /locus_tag="IWI97_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBC2461964.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16313..16783
                     /locus_tag="IWI97_000432"
     CDS             16313..16783
                     /locus_tag="IWI97_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBC2461965.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16800..17102
                     /locus_tag="IWI97_000433"
     CDS             16800..17102
                     /locus_tag="IWI97_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461966.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17119..17490
                     /locus_tag="IWI97_000434"
     CDS             17119..17490
                     /locus_tag="IWI97_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBC2461967.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17462..17821
                     /locus_tag="IWI97_000435"
     CDS             17462..17821
                     /locus_tag="IWI97_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461968.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17993..19000
                     /locus_tag="IWI97_000436"
     CDS             17993..19000
                     /locus_tag="IWI97_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2461969.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19025..20209
                     /locus_tag="IWI97_000437"
     CDS             19025..20209
                     /locus_tag="IWI97_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBC2461970.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20646..22229)
                     /locus_tag="IWI97_000438"
     CDS             complement(20646..22229)
                     /locus_tag="IWI97_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBC2461971.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22807..22950)
                     /locus_tag="IWI97_000439"
     CDS             complement(22807..22950)
                     /locus_tag="IWI97_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBC2461972.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23173..23652)
                     /locus_tag="IWI97_000440"
     CDS             complement(23173..23652)
                     /locus_tag="IWI97_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBC2461973.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23725..24009)
                     /locus_tag="IWI97_000441"
     CDS             complement(23725..24009)
                     /locus_tag="IWI97_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2461974.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24087..24281)
                     /gene="ssrS"
                     /locus_tag="IWI97_000442"
     ncRNA           complement(24087..24281)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWI97_000442"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24353..25639)
                     /locus_tag="IWI97_000443"
     CDS             complement(24353..25639)
                     /locus_tag="IWI97_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBC2461975.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25777..26448
                     /locus_tag="IWI97_000444"
     CDS             25777..26448
                     /locus_tag="IWI97_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBC2461976.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26414..26902
                     /locus_tag="IWI97_000445"
     CDS             26414..26902
                     /locus_tag="IWI97_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBC2461977.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26915..27862
                     /gene="prmA"
                     /locus_tag="IWI97_000446"
     CDS             26915..27862
                     /gene="prmA"
                     /locus_tag="IWI97_000446"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBC2461978.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27863..28627
                     /locus_tag="IWI97_000447"
     CDS             27863..28627
                     /locus_tag="IWI97_000447"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBC2461979.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28860..31073
                     /locus_tag="IWI97_000448"
     CDS             28860..31073
                     /locus_tag="IWI97_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBC2461980.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31084..31530
                     /locus_tag="IWI97_000449"
     CDS             31084..31530
                     /locus_tag="IWI97_000449"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBC2461981.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31701..32960
                     /locus_tag="IWI97_000450"
     CDS             31701..32960
                     /locus_tag="IWI97_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBC2461982.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33049..33996)
                     /locus_tag="IWI97_000451"
     CDS             complement(33049..33996)
                     /locus_tag="IWI97_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBC2461983.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFRETLTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34390..35682
                     /locus_tag="IWI97_000452"
     CDS             34390..35682
                     /locus_tag="IWI97_000452"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBC2461984.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35697..37466
                     /gene="aspS"
                     /locus_tag="IWI97_000453"
     CDS             35697..37466
                     /gene="aspS"
                     /locus_tag="IWI97_000453"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBC2461985.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37575..38450
                     /locus_tag="IWI97_000454"
     CDS             37575..38450
                     /locus_tag="IWI97_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2461986.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38716..40599
                     /locus_tag="IWI97_000455"
     CDS             38716..40599
                     /locus_tag="IWI97_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBC2461987.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40674..40958)
                     /locus_tag="IWI97_000456"
     CDS             complement(40674..40958)
                     /locus_tag="IWI97_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBC2461988.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40948..41682)
                     /locus_tag="IWI97_000457"
     CDS             complement(40948..41682)
                     /locus_tag="IWI97_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBC2461989.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41811..42434
                     /locus_tag="IWI97_000458"
     CDS             41811..42434
                     /locus_tag="IWI97_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBC2461990.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42626..43747
                     /locus_tag="IWI97_000459"
     CDS             42626..43747
                     /locus_tag="IWI97_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBC2461991.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43754..46882
                     /locus_tag="IWI97_000460"
     CDS             43754..46882
                     /locus_tag="IWI97_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBC2461992.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46901..50095
                     /locus_tag="IWI97_000461"
     CDS             46901..50095
                     /locus_tag="IWI97_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBC2461993.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50241..50948)
                     /locus_tag="IWI97_000462"
     CDS             complement(50241..50948)
                     /locus_tag="IWI97_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBC2461994.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51713..52111
                     /gene="spxA"
                     /locus_tag="IWI97_000463"
     CDS             51713..52111
                     /gene="spxA"
                     /locus_tag="IWI97_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBC2461995.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52443..53096
                     /locus_tag="IWI97_000464"
     CDS             52443..53096
                     /locus_tag="IWI97_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBC2461996.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53322..54320
                     /locus_tag="IWI97_000465"
     CDS             53322..54320
                     /locus_tag="IWI97_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBC2461997.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54496..56304
                     /gene="pepF"
                     /locus_tag="IWI97_000466"
     CDS             54496..56304
                     /gene="pepF"
                     /locus_tag="IWI97_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBC2461998.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18551 a         9728 c        11685 g        16419 t
ORIGIN      
        1 caaaaagatg aactagaaat agttcatctt tttttgttta aatcttttgc taagttgaac
       61 taatagagtc ttttatgaat atcgaagtta gtttttcacc taacttcgtt ttttatagtt
      121 ggttattata aaatagtaat atagctgatt tggattcaat tcatatttat ttataaatca
      181 aaaatacaga gaatagtata taaatgtcct ttcctattct taaaatgatg tttgtactta
      241 cttaaattag gtataattag tatatgtttt gtgagaaatt tagacttaca ataagcagtg
      301 aatttataat caacgtatag gagacatgga tgaaatgaaa ctaattggta tagatttaga
      361 cggtactctt ttgaattctg accagagcat tagtcaaaaa aatgcaagaa caatgaaaca
      421 tttaccggaa gactgttttc cattcatttg ttctggaaga gaagtagaag acattaataa
      481 tattctcaaa aaatctggtt taaggatacc agcagtaggg ttaaatggtt ctgttggatt
      541 tgatggagaa aaaaagatat ttgagtcttc cttcgataaa aatgatgtaa aaaaaattga
      601 ttctgtccta tcgaaatttc caaccaaaat atatacaaat ctcggaagtt atgaatctca
      661 gaattataaa aataaaatga aaaaaatttt tcacgaggta gggagtgaat tttctatcaa
      721 agagctaaac tatgagcttg agtatgaaaa atctatttgt tcaatccctt ataataatat
      781 tgaggaaatt atagaaaagg atgctatcag catttacaaa ttttttattt ttattcctaa
      841 tcgtcaattg aaagctaata tatttaacca tttgaaaaag atgctaaatg tgtctgttac
      901 tgaatcggca cctgtaaata ttgaaatcgt acctcaaaat gtatctaaag gtgtggtgtt
      961 tgatcgtttg gaatcaatct acaatttaaa gaaacctttg agaatcgcga tcggagatag
     1021 tttaaatgac ttatctatgt ttgaaagtgc agatattagt tttgcaatgg gaaatagtca
     1081 tcaagaaatc atgaaaaaag ctgattatgt cactgtttca aatgataaag acggagtatc
     1141 tcaagcattt gaaagaattc ttaccatata aactaattaa ggtagtatag aacatagaat
     1201 tctaaatacg gaaaaatgga gcataaattc cagtacaaat agtacttctt cagtgtcaaa
     1261 cgaagcgaat ataacataga tatttaggat tgtgacaaaa atcatgtcac aatctttttt
     1321 tcgaataaac ttattttttg aagtcggcca tttatcgtaa tttctttgca taatacgaac
     1381 aaattttcgg ttattgatga aaaaaaacat tgaaaatggt atgataaaag aaaaaaagga
     1441 ggaaattttt aatggtgaaa tttatccatg cggcagattt acatatggat cggtcatttg
     1501 aaggactgac aactcttgat aaaactgtac aggaaaaact attgaagacg aatctgaccg
     1561 ttttatcaaa tatcattgaa caggcaataa taaataacgt tgattttgtt ttacttgcag
     1621 gagataattt tcatcaaaat cgtccttcat taaaaataca gaaacatttt tcagaacaga
     1681 tgaaacggtt agaaaagaac catatacctg tatttataat attcggaaat catgatttct
     1741 atcaaaaaga gcggtattgg ttctcctttc ctaaaaatgt ccatttattt acatccgaaa
     1801 cggtagagac aaaaaaaata acgataaaaa gtggagaaac tgtctctctc agtggattta
     1861 gttatcgtca accgtggatt caaaaagaca aagtgatgga atttccttct agagaactca
     1921 cagattacca tattgggttg taccacgggg aacctggcgt ctctcaaaaa ggaaactatg
     1981 ctcctttcca accctcaaag atgcaagaaa agggatacga ttattgggct ttagggcata
     2041 tccatgtgcc aacggtctta aatgaaaagc aaacaattgt ttatccaggt gcgcctcaag
     2101 gtcatactaa aaaggaacaa gcttctacat ctattctact agttgaatta agtaaaggaa
     2161 gttgccaaat acatccgatt aaagtagcag aggtctattg gaagacaaag gaaatatcat
     2221 tgaggaaagc aagaacgaca aaagaggtca ttgttcacat tcgtcagcag ctttcaaaag
     2281 tagaggatgg atttagttta attgagataa aattaaaaga ttatgaccat ctcaatacag
     2341 atgttttgga acggatacaa tcaggagagc tattagacta tttgttagag gaattctcag
     2401 ataggataaa tgatttcttt atttggcgta tttctctcat agagaatact ttttatacga
     2461 agaccccact tgctgcttct gctaaattga tggaacagtt atttcaatac tatcagacac
     2521 ctcaagattt ccaacaaatt ttaaatgaag tatatagtca tcaagaagcg gctagaatat
     2581 tgagcgaact gccggaatat caagaagaaa cacttgaaaa agccaagcag ctgcttaatc
     2641 aagatttttt atttgaggag gatcaggaat gaagatttta aggatcgaga ttgctgcttt
     2701 tggaaaatgg cgtcaaaaaa gttttgactt ttattcaggc aatcagctga tctatggggg
     2761 aaacgaagca ggaaaatcaa cgatttatca atttattcaa gcaatattat ttggtttccc
     2821 ttcaaaaggc cgtaaaaaaa aggattatac accaaaagac ggctcagcct atggcggcaa
     2881 aatatggctt aaacatcctg tatatggtga atttgctgtc gagcgctaca agcagcaaaa
     2941 tcgcggaaaa tcgaaggtgt ggctggggga tcaagtggga agtgatgagt tactagaaaa
     3001 actgatcgca cctttgacga aagagttatt ccagcaggta tttactttcc agcaagagca
     3061 gttgatggaa ctagatcatt tgagagaaaa agagctacat gatgcgctga tttctttagg
     3121 catcacgggc agttctcaag tattccaaaa gcggcaagaa tattatcaga aagcacaaca
     3181 attattcaag aaaaaaggcc aaaaattact aatcaatcaa aaattaaatg aatggaaaga
     3241 gctgcaagaa aaaattcatc aaaaacaaga acaggaagct tattttggca agttgatcca
     3301 acaagaacag gaatacgagt cgcaacagca gcagttattg gcacaattaa aagaggcgaa
     3361 caaacaattt tcattagcca gacagcaaga acttaatttt tctttatatg aagaatggca
     3421 atctctcaaa aaaaacagca ctacaggtct tcctgatgag caacttcttt tagatctgga
     3481 gtctttctcc aaacggtatc agcaaacaaa tgagcgtttg gaagaattag aagcaacact
     3541 ggaaaagcat agcgggatgg atcagcagtc agccagatat tatttttatt tagagcagga
     3601 gcaggaacta aaagatctcc agcaacttca atttacagcg gaacaattaa acgaagaatg
     3661 gcaaagatta caaattgaga ctagagaaac aaattcagaa ttgaagattt tggaacaaag
     3721 gtggcattgg cagaaaaatc cgttaccgga agaattttta tatgaagaag aatggaagca
     3781 tcttctggct gaagaaagaa ggattgctga tctagaaagc cagatttctg tgcgtctcca
     3841 aatagctaaa gaacagcaag agatgattga agcagaagtg aatgacttgg aagagaccta
     3901 tccagcatta ttacaaaatc agtcagagat agcaccagtc tcttcatcta aaccgtatat
     3961 gctgatggct ggcggagcaa taatcattgc aggtatattt gtaccaatgc ccttgaaagt
     4021 gatcctttta ctaattggtg caggcatgat tggctatttc ttctacaaaa ggcaaccaaa
     4081 aattaaaact aaaaatgatt tctctgagat aaaaggaatg tggcaagaaa aactaggaca
     4141 acttgatcta gccttagctc aattagctga agcagaagaa aaacaaaaga gaactggaga
     4201 agaaaaacag attcttcagc aagaagtata tcgttttgcg gatcatcggc acttaggaaa
     4261 gatggatacg ctcaaactca tgaaagaata tggacaggaa gtcatagatt atcagaaatt
     4321 atatggcgta tttgagcgga aacataaaag agaacaggaa atacaagaaa aactacgaca
     4381 aatcgaccaa aaatttgagt ttgttaaaga atggttaccg atacaagata agacaatttc
     4441 agaaaaaatg gtattgcttt cccgttttat acaagagatg gaacagatga aatttgccag
     4501 aagctatcag caaaatactt tattgaagca agaaatcaat caattgaaaa agaaacaaaa
     4561 agtatggata caagaataca gcgaacaatt gactcaagca ggcttatctt atccttcgga
     4621 agtacctgtc tttttacaaa aagccaaaca agccgaacag cagatgaaaa gatacaatga
     4681 attatcggaa atgcttactc cactatttcc tgaagagctg acaaaagaga aattatctgt
     4741 aagactgcgc caactgaaag aaaagcaatc ccaactccag caagaagaaa aagaagcaag
     4801 tgaaaatcgg caacgactcc aacttcaagt agaagagttg caaagagacg gcacattgga
     4861 cgaactttat caacaagcta gccggcaaaa agcaaaatta caggaattac tggaggaatg
     4921 gggagcactt gaaatcgccg ggacattctt aggtgatctt tcaacagaga tgagtgaaag
     4981 acaattaccc cagttgttaa gagcagcagg tgattattta ggtattttaa caaaaggaaa
     5041 ttatcagaag atattactga aaaatgatac actgacttta acagacggat atacagaatt
     5101 tcccatctat gagttatcta caggtacgaa agatcagctg attatggcga tgagatttgc
     5161 ttacctctca ttagaagcta accgttcgat atgcccgatc atcattgatg atggctggct
     5221 tcattatgat catcagcgaa agtatcaatt agctgaactt ttagctaaat ttggagaaaa
     5281 gtatcaaata atttgttttt cttctgataa agaaatggta agctattacc atgagatgaa
     5341 ccaaatcgta aaacaattag aaggagcaaa taatgaaaaa aattcgtgat ttagtaatcg
     5401 atgagcagtt tgaacaattt gtcctgatca aaagtgcaga tgtaagaatc gctaaaaacg
     5461 gaaaaaaatt tattgctttt actttccaag atacttcagg tactatcgat ggtaaatact
     5521 gggatgcttc ggaggaggaa atcaataaat ttacagccgg cacagtcgtt tttctaaacg
     5581 gtaaaagaga ggtttatcaa ggaaaccctc aagtaaagat tcttcatatg cgcttaacaa
     5641 gggtagatga gccaggagag cctagtttat acatggaaag agcccctttg aagaaagaag
     5701 aaatggaaga agaaatcaat caattgttgt ttgagatcac caatgcgcat tggaatcgta
     5761 ttgttcgcta tctacttaat aaatatcaaa aacaattttt tgagtttccg gcagcaaaaa
     5821 gaaaccatca tgcatttgcc agtggattag cttatcatac tttgacaatg cttcacttag
     5881 ggaaatcaat cgccgatgaa tatcctgagc tgaatcgagc attgttgtat gctggtatca
     5941 ttttgcatga tttaggaaag gtgattgaac tttccggtcc tatgtcgaca gaatatacgc
     6001 ttgcaggaaa tttgatcggt catctggtat tgattgacga agaaataact aaagcctgtc
     6061 ttgcgttgaa aatcagcgaa gctgacgaag atgttctcgt attacgtcat atggttttag
     6121 cacaccatgg cttattagaa tatggctcac ctgtacgtcc aagaataatg gaagcagaaa
     6181 tattacatca aatcgacaat atcgatgctt cgatgcagat gatgttgact tcgattcgtc
     6241 aaacagaacc aggaaaatac accgatcgga ttttcggtat ggacaaccga agtttctatg
     6301 taccaaaaga tttatagcaa aaatttattt ttttcattca aaacaaaaaa agcgagacat
     6361 aagtcttcgc tttttttgtt ttcttttatt ttgatgaatc agttgtttct tcagttgaag
     6421 attcttttgt tgaatcagtt gattttgtgt cagaactctt tgttgaagct gtagtttcac
     6481 ttgagtcttt cgtacttgat gaagttgttg tgaatgcact caacacattt tcaaaggcat
     6541 catctttgat ttttacattt gcatctttta attcttcacc gattacttta gttgtgaatg
     6601 cattgtcact tagtttagtt tcagtagcga tatcttttag ttgatctttg tatttatcca
     6661 tgtcattgcc tttattttgg tttttcacca ttttgacaac gtagtattct gtcgcataag
     6721 aagtaggatt tgttgtagta ataacatcgg agatctcacc gtctttcaat ttgaaagctg
     6781 cttcttttac ttctgcagga atagttgttg tcgttgagtc gaatttaact ttaccgccat
     6841 cttcttttgt ctctgtatca gttgatttgt ctttggctaa tttagagaaa tcatctccat
     6901 catccgctga ttttttcaca tcttttgctt catcttcact tgataatttg atgatttgtg
     6961 cttctacttc tggatggaaa gatttccaag cagttttcaa atcgtcatct gtaatatcta
     7021 cgtgagcttt caagccagct tcaaaagcta aattgttacg aatatattct ttataagtat
     7081 ctttcgtata tccagcagct tctaattgtg attcaaaagt atcacctaat gatttcgctt
     7141 gtttgtcgta ttctgcatcc acttgcttat cggttacttt atcgccatat ttgttattga
     7201 atactttata aatgatcatc cgttgtacta aggattggtt cgatgattca agttttgctt
     7261 catcatagaa atcagataca gtgatcgttc cgcctttcat cgttgcgata tctttattag
     7321 tatcgcctga acaagctgct agagttaaaa cagccaacgc actagtagct gccaaaataa
     7381 ttgatttttt tttcattaaa atttgcactc ctgtaattag ttattatttt tatatattaa
     7441 ctataccata tcccatttcc tttaaaaacg ttgtcgtggt agtttcaaac aaaattcaaa
     7501 atttcatcac attcctagga aatacgcaaa cttttctcac ttatatagtt aaatctgttg
     7561 agaaaataat cattattata tttgttcagg atactctggc agatcagctt ttagtttttc
     7621 tacttgttca ttgatttgtg tgatacgtgg ttcggcttgg aatttaaaat cttcgatatc
     7681 ttttttgatt gcttcttgga aagcaggaat cacagattca gctgtttctt ttgtttcgat
     7741 caatgcgctt ttaaaatgat tcaggctgtt atttaaatca tttgtcagag ttgttgcttc
     7801 ttccaattct tcgatcagtt tctttcttgt ttcattcccg ctcctgggcg ccaataacag
     7861 acctccgaca gtgccagcag cagctccaaa taacagtcct ttgaaaaagt ttttaatcat
     7921 cgcaggacac ctgctttacg attgtttctt gaatagcttt tagttcttcc gatccataac
     7981 tatcttgatg attgacaaag tgaatagaaa aatcatcctc tttgctgtag cgagggacta
     8041 aatggatatg tgaatgaaaa acagattgat aggctagttc acggttgtta ttcaagatat
     8101 taagaccttt catttctgga aaagcttttt ccaatgcttg agcaatctta ggcacacgcg
     8161 aaaaaacctc tcctgcaagt tcgggatcat attcaaaaat atctgctaca tgacgtttag
     8221 gcacaactaa tgtgtgtcct ttcgttgctt gagagatgtc taaaaaagca tagaccttgt
     8281 cgtcttcata tattttatag cttgggattt cttgattgat gattttgcaa aaaatacagt
     8341 tttccatata aaacatcctt tctataaaga ataaatactt cagcttaact ttaccataag
     8401 ttaaagctat tgtaaggaaa aaaacaaaat accgtcattt tcttcgtggt ataataaata
     8461 aaaataaatg gcggaggaaa aagaatgagt ttaattatcg atcatgtcac aggggggtac
     8521 ggacatctcc ctgtgttaaa agatatcagt ttcgatgtac agcctggaga gatagtaggg
     8581 ttgattggac tgaacggagc agggaaaagt acgaccatca agcacattat cggtctgctg
     8641 gaagcttcaa aaggaacgat agaaatcaat catctatcct taaagaaaaa tacggaagaa
     8701 tatcgcaaac aaattgggta tattccagag actccttctt tatatgaaga attgacattg
     8761 agagaacata tcgaagtcac tgcgatggcc tacgatatac ctatggatat agcaatgaaa
     8821 agagcagaaa agttgttgga gacttttcgg ttatctaatc gtcttgactg gttcccagct
     8881 aatttttcaa agggaatgaa acagaaagtg atgatcttat gcgccttttt gatcgagcct
     8941 tctttatata tcatcgacga accattttta ggcttggatc cactagctat caatgcattg
     9001 cttgaattga tggtagaaat gaaaaaagaa ggagccggta ttttgatgtc tacgcatatt
     9061 ttagctactg cagaaaaata ttgtgatcgt tttgtggtct tacataacgg agagatacga
     9121 gcagacggtt ctcttgagat gttgagaaaa gaatttcagc tgccggattc ttctttggac
     9181 gaaatctata ttgctttaac aaaggagcag gcacatgagt agtttttttc aaaaacgttt
     9241 agcaagacat caaaagaaaa tgatgaaata tatgcgttat gtattgaacg atcattttgt
     9301 tttagtttgt cttttcttga ttggcggtgt gggattatat tattctaatt ggttgaaaac
     9361 cctgcagcca ccgtttgcaa ttggagggct cataatctct gtattttgga tgctctgttt
     9421 attcgtgggt aagatggcta cttttgcaga ggcagcagat attgtttttt tacttcctaa
     9481 agaaaaagaa atgcaaacct atttggaacg agcttttcgc tattcttgca tttttccgtt
     9541 gatctttttg atattggcat gcggattggc catgccatta gtggtggtta gtacggagca
     9601 atccttttcg atgtttttcg tctatctgat catattatgg agtttgaaat tcagtcatct
     9661 gcaaatcaag agagcagaat tgtttcgact agatcaacaa gttattcgtt attggaaatt
     9721 aggctggttt ttttcttctc tgatcttttt gtttctatca ttttattggc taatatgggc
     9781 aggaatagca ggtgcattag tacaggcaag tctatattac tggtttttgt ggaagcaaca
     9841 agaaaaagga ttggattggg agaaaatggt gcatgtagaa gaacataggt tgcatcagat
     9901 ctatcaattt atcaatttat tcacagatgt tcctgaaatt acggcaaaga tcaaaagaag
     9961 acgctatttg gatcctatat tgactcatat taaaagagaa tcaaaaaaca cttatcttta
    10021 cttgtttgca agaagattgg caagaggttc tgattttagt ggtctttatt tccgattagt
    10081 aattataggt gggttattga ttgccggagt agtcgacttt tattttagtc tgattatcgg
    10141 tttcttattt ttatatttga tcggtttcca actgttacct ttagcgaacc agttccgcta
    10201 tatggttctc gttcagcttt atccaatcca taagaatcag aagaagcagg cgattcagta
    10261 tctactcaca tgggtattaa ttatcgcgtc ggttattttt ggtttgattg ctgcagtcgt
    10321 attagatggg cagcagcgat gggttccgct agcggcttat ttgattgtta ctatgctctt
    10381 tgttaaacta tatgtaccaa ctcgtttgaa gaagatgtca gactgaaaac aaagctgtgt
    10441 agggtaaaaa tgagaatgaa aaatagagcg ttgacagtaa gaatgctgtt cgttctattt
    10501 tttttgagaa aagctggttt tttgaatggc aattaaatga aaaagaacgt atatgtgctt
    10561 gacgtgtagt gttcaggcga ctagaataat aagtactgca aaacaaagag taagaggacg
    10621 ggagaatgca tgatggagtt tcagttagac caagagtggc ggttacgtcc catcaaaggc
    10681 gacacaggaa aaacatacat aggcatgcgt gatgaggaca aagtctttat taaacgaaac
    10741 acgactccga tgttggctgc tctttcaagg gaaggaatta caccaaaact tgtttggaca
    10801 aaacgaacgg ggaatggcga tactttgaca gcacaagagt ggctggaagg tcgcttgctg
    10861 actcctaatg agattggtca aagaaacgac gtgattgatg ttttgtacca tttacatcat
    10921 tcaaattctc ttaaaaccat gctgacccgt atcggtggtc gtgtcatgac gccggagaag
    10981 atgctgcaaa aatatgaaaa agaattaccg gaagaactcc agaaaaatca atttctgcaa
    11041 attgtccatc gttttcttcg tgagcagatc ccgtcaatag ataaggaaaa cttatcagta
    11101 gtacacggag atgtaaacca tcgtaattgg ttagtatgta aaaattattt atatctagtc
    11161 gattgggatt caatcatgtt tgcagaccct gcggtggata ttgcaacgat tttaggaaat
    11221 tatgtgccac tgtctagctg gaatcagtgg ctgatcagtt atggcatacg cccgactgac
    11281 gaaatgcttg aaaaatttca ttggtatgcg gccatgaatt tgctccaaga aatcaccaga
    11341 tattgcaaac gtgaagatca tcgacggatg aacgaagaaa ttttacaatt aaaacgaatt
    11401 ttcagtggct gaggtttggc ccaaactctg cggagatttg gccagacctt tttaattaga
    11461 ggaaaggaat ggagataaat aatgcgtgtc cgaaatcgtc cgggagcagc ggaaatgtta
    11521 gcggctcacc cgaattttgt aattagtgat ccaacattgt ggaagggaaa atggaatgaa
    11581 ttgtttgaaa acgatcatcc aatccacatt gagattggta tggggaaagg ccagttcatc
    11641 actggaatgg caaaagccca tcctgaaatc aattatattg gagtagaaat gcaggtcagt
    11701 gttgtttcga ttgcactaga caagttgatc gaacaaccgc ttcctaacct gaaacttcta
    11761 catgtggatg gatcagcgtt gacagagtat ttcgctgaca gcgaagtaga tcaaatctat
    11821 ctgaattttt ccgatccatg gccaaaaaaa cgtcatgaaa aacggcgatt gacatataag
    11881 acttttcttg ctgtagatga acagattctt cgtccaaatg gagaaatcca tttcaaaaca
    11941 gataatcaag gattgtttga gtattctctg gcaagttttt ctcaatatgg catgatcctc
    12001 aaacaggtat ggttagattt acatcaaagt caatttgaag gcaatattat gacggagtat
    12061 gaagaaaaat tttcttcaaa aggtcaacgt atttatcgag tagaagctca atttcaagat
    12121 aagaccaaaa agaaaagtct gtgaaactaa ctttctgctt tcataaaaac attaatatcc
    12181 ataaataaat cccagaaaat gaacattaca gatgtaatgt attttctggg atttatttga
    12241 tttaaaatgt atttatttca tcggtttttc cacaaggtgg aatcaattgg aagcgagaaa
    12301 atgaaaataa tcctttgtcg gtgatcttca attcgggtat gacagataat gttaagaaag
    12361 aaagtgtcaa aaaaggatca aaggttttag ctgctcctag ttgatgtgct ttagttatta
    12421 attcattcaa ctgttcatag acgatcgaaa aaggttcctg tgtcatgatt ccgcctatag
    12481 gaagagaaac acaagctatt tcctgctgtt tgttgacaac ggtcatcccg ccaccttttt
    12541 gaatcaaaag attagcagca tacagcatat cctcgtcgtt tgtcccaacg acaactagat
    12601 tatgcgagtc atgggcaatc gttgtcgcta tcgctccttg tttcagctga aatcctttca
    12661 ctatgcctaa tccaatgttt cccgtttgat ggtgacgctc gatcaccgca atctttaatt
    12721 ggtctttttc gattgaagga atgaatcgtt cgccttgtcg gtccactttt tctaccaaat
    12781 ctttagtcac tagactattc gggataattt cgatcacatg ggcttgtgaa gaagaaagaa
    12841 gcagctctaa cgaatgtgga tttaatggtt gagcgtttag ttttggcaga caacacgtat
    12901 atgaatttga atgatcagca aataaatggt cttggagttg cccagattga acaactaatg
    12961 ttcccttata gaaaacttca tgaattggca gcttggtaag atcgtctgtt aagaagaaat
    13021 cagcttgata cccaggggca atcgcaccta gtgttttcaa atcaaaacat tctgctgcat
    13081 tcaatgttgc catctgaatg gcttgtaatg gatcgattcc aagtgcaata gcttgacgga
    13141 cgatatgatc gattgatcct tcttttacta aatcattgat cagtttatca tcggtgacaa
    13201 agaagcatct tcttgaattt tcaggtgtga tcgccggtag tagcgatgcc agatcttttg
    13261 caacggtccc ttctcgaacc attaaataca taccaagatc caaacgttct ttagcttctt
    13321 ttgcagttgt agcttcatga tccgtccgaa tacctgctgc aggataaaca tttaactctt
    13381 ccatgccgat accagctgca tgcccgtcaa tttttttctt atgctggtgc atcaattgga
    13441 tcttttctag gatgtctgtt tcattatttg ctactgcttg atagttcatg acttcagcta
    13501 agcctaaaac ttcgggtttt tgtaaaaaag gggctaattt ttttgcattt aaatttgcac
    13561 cattggtctc aaaaggggtt acaggtacac aagaaggtag cataaaaaag atattcatga
    13621 gtgtttgtct tgcatcttct atcataaact caagacctgt actcccagca atgtttccta
    13681 tttcatgggg atctgtcact acagtagtaa ctccatgaag caaggatact tttgaaaatt
    13741 cgcttggtga taaaagtgag ctttcgatat gcacatggcc atcgatgaat cctggcacta
    13801 gatattgccc attagcatta tgtacgcttt ttccttcgta ttcaccgatt cctacgatca
    13861 ttccttcgca gattgctaca tctttttcca tgatttcttt cgtaaagacg ttgacgatct
    13921 tagcatgctt gatcactagg tcagcaggct tttttttact tgctacttcg atatatttcc
    13981 ttaatgttgt ttccaatgga gtgcctccca agataaattt ttataagtat aacataaatt
    14041 cggtttttgc caatattttt ttgtaaattc attttatttg aaacaaaaag atcaaaaaga
    14101 cgtatgtttt ttgtggaggg gagaataatg gaaataaaac gattatcgga tcaaatgata
    14161 agtgaaagtt ttgccaaaca tgcccaagac gtatatatat tgccagcttc agaagtattt
    14221 tacatctatt ttcgctatgg aaaaaaacga acattctgga agaaagtctc ttcggcagat
    14281 ggacagcaac tgatctcaag atttaagtat ttgggacaga tggatatcgg agaaaaaaga
    14341 aaagctcagt taggtgcatt tacttattct ttagaaaaac aggaagttcg tctgcggttg
    14401 tcttctgtag gagattacct tcaacgagag agtttagtga ttcggctatt atacggtact
    14461 tttgaaacac cccatcgttg ctttagttct cgagattttg caactatttt acagaaaacc
    14521 gtcagacgtg gattatacct tttcagcgga ccagtcggat caggcaagac ttctctaatg
    14581 tatcggttgg ctttggaaga aaaattgcaa gtgattacta tcgaagatcc tgtagagata
    14641 gaagaaccgt tttttctgca gctacagatc aatccgaaaa tagcacagac ttatgataca
    14701 cttttgagac tcgcattacg gcatcgtcca gacttgctaa tcattggcga gataagagat
    14761 gaatcaactg cacatgctgc ggtaagagca gcactaacag ggcaccgggt ttttgcaacc
    14821 gtacatgcac gcaatcttga aggtacattg gtcagaatcc atgaactgac aaagaaaaaa
    14881 gaagagctat ctgaatgttt agctggtgtc atttatcaag aacttttgga agatgccaat
    14941 caccatcctt ctgttttatg gagttatcaa tttatggagg aagaatcagt aaaaaaatca
    15001 tgggaggaaa gttatgaaga agctcaggaa aatatttggc agtcagaact attctaaagg
    15061 attaacaaga agccagcaaa accagtttgc acaaaccatg ggagaactgc ttttatctgg
    15121 ttttagtctg caagaatccc tttatttatt actcacgatc cgttcgtttc cacctgttat
    15181 tttggcaaat atccaacatc agctaaagga agggcaagcg tttagtgagg tactttcgca
    15241 aataggatat ggagcagagc aactggtaca gatcgagtta gctgaacgtc atgggaatct
    15301 gccgacaact ttgttggaaa ttgccaaaca gtttcggctt gttgaaggat ttcgcaatga
    15361 tttgaaaaag ctgcttgcat atccgatgct attgctcacc tttttgctag gtatcttgtt
    15421 tagtatgcgg ttgttcgttt tgcctcagct tattggttca ggaatggtca tgcaagagca
    15481 ttggggtatt cagctgatac aagtctttca ttggtatgtt ctactattca tcctagcggt
    15541 tactctattg gctttactag taaggtatcg tctgatgaag ttgtctcctg ttgatcagag
    15601 tgaatggata agcagacaat tattgattgg accgatatat tccttgtatc aatcggctta
    15661 tttgtcacta gagattggaa agctttttca tgaaggtttc gaattaaggc aaatcatagt
    15721 ctgcttaaag gaaacgaaac aagaaagtct gattcagcat cttgcctcta agttgattca
    15781 aggattggag gcaggtatgt cattggctga gcagttccag aattatcgtt ttctatcaga
    15841 agaattcagt aaaattgttt tacagggaga ggcaaaggga agtttaggta aagaactgat
    15901 tttttacagc gaatggacaa gaaaacagct tttcaaacgt cttcatcact ggatgcattt
    15961 tattcagcca atcatttttt tgggcgtagc ggctttgatt ttattagttt atgccgctgt
    16021 tttattacct gtttacggaa gcatagagga ggttttgcca tgaaaaagag aaaaatatct
    16081 tattatagcg gttttacatt gatcgaaatg ctaatcgtct tactgatcat cagtgtatta
    16141 gtgttgttgt tcgtccctaa tttgtcgcgt tataggaatc atgtagacca agaaagtcgt
    16201 gaagcgatca tccagttggt agacacacaa aaagagctct atgcgcttca aaataatgga
    16261 agagttccta ctgtggagga attgttgaat gaaggctata tcaaacgaga acatgcagaa
    16321 atctatcagc ggccttgaag gtaaggtttt agcatcagga tatacgttgt ttgaatcact
    16381 cttactgatt ttattactta caacgatcct ttcttttcca gttctcgcat tttctgcatg
    16441 gaagaatgaa ttggcagtct atcaattttt cggccaattt gaaaaacgaa tctattctac
    16501 gcaaaaaatc gctattgtga atcagatcca aacaggcttt tattggaata gtgaagagaa
    16561 tcaaatcatt tttcatgttc cgaatccaga taaaataaat tgggaagtat tagatattcc
    16621 tgatgagatt accttgaaac gccatgcttc tattactttt gctgctagaa ccgggaatga
    16681 aagttcacta aaagcttatc aattttattg ggaagaaaaa gagcggacga ttacgtatca
    16741 attccagata gggagtggtc gctatacaaa aagaattgaa taaaacagga tttgttttgt
    16801 tggagtccct tacagcgctt atgatcgctt ctgtctgtgt ctacttagtg agctttggcc
    16861 agctgttttt gcttcaggca gaaaagaaaa gtggggagga actgctattt attcgaatga
    16921 tttatgaaga tatcaagacg cagcggatgt atcacaaaag accagagcga agggaagaaa
    16981 tgatcccata cgatatccaa attgtcgata ataatggagt aatcgaaaaa gtgtttgtca
    17041 atatggagaa agaagcgatc agtattgaaa aaacaagtaa ttccagcatt tacgctgatt
    17101 gaatgtctaa ttggactgat gatattgagc agtctattgc tcacattcaa tcttttgatt
    17161 cagcaaacca tccaagtaag tgaatttttg cagcgtaaag atcaaaaaga gtggctaata
    17221 tttttagcac aattagaaga agaactcaag agcagtcaca atgtctctgt gaaaaatcag
    17281 caactgcact acacgatcga ggataaacag tatatcatcg aaagatatca ggcaatgata
    17341 cgtaaaagaa gaacttccgg cggacatcaa cctatgttaa caagtatcac tgagcttcaa
    17401 ttattggaaa aagaacacac aatttctttt tatgtacact ttgaaaatgg agaagatgga
    17461 tatgccatat ggacaaaaaa cgatcagtag aaaatgttat aagggctcta ttttattttc
    17521 tgtcttgctt ttatttcttt tatgtacgtt tttattgatg gcagcattag aaagctaccg
    17581 tttgtcaatg gatcttaata tacgaatcaa aaattattat atagcaaaaa taatgaagca
    17641 gatggcttta tcggaacagt ctacgcttgc cgaaagttcg gaaggagttt tttactacac
    17701 aactggatct gtaacatatc agtgggtaca acagtcccta ttcttagagg tggaagtctc
    17761 accgtttatc tttcggttca cagaagaagt aaaaaatgat acagatacta gtacagaatg
    17821 atggaatgtt cgtgaaaacg ccctcttttc tgttataaca ctgtttttaa taaaaaaatg
    17881 tttgccgttt cacaaataaa aaacagtgaa aacgattgaa attttgtcaa ttgttcgata
    17941 gaattaaaca ggtgaaaaaa ttgttataat atatctagaa atgaggtgca ccatgttacc
    18001 agaaaaaatg gaacaagggt ttaatcaaaa cctggaagct attcagcttt tacagaatgc
    18061 attgggaact tcctttttag aagcctatgt cgaaaatgca gaaaatttaa tagatgatta
    18121 tcaagtacgt gtggtggatg gagtgccgac aaaagagaca acgcaacgga tcacagctct
    18181 ttatgaagag ttgaagaaac tttctttcga gccagaagaa tggcgcagac tgtctcaatt
    18241 acttttattg aagggcagcc agacagaaca tttgcaggcg aaccatcaac tcactcctga
    18301 tagtatcggt tttttatttg tttttcttat tgaacaacta tacacaaata aaaaagcacc
    18361 ggttaagatt ctggatattg cggcaggaat gggcaacttg ttattaacag ttcttttaaa
    18421 cctatcgaat gcggggtacc aaacagaagg aattggtgtg gatattgatg atacattgtt
    18481 ggctgtcgca gcttctacaa gtgatttgac acaagcaaat gttcaatact ttcatcaaga
    18541 cgggttgcag gaattattaa tagatcctgt cgattttgca atcagcgatt tgccaatcgg
    18601 ttattatccg aatgacgaaa aagcaaaaga gtttttaaca agtaccgaag aaggccatag
    18661 ttatgcacat catttattgc tagagcaatc aatgaaatat gtgaagccgg atggttttgg
    18721 tttgtttttg atgccgagtg gatttttaga aacggatcaa agtgaagaaa tcaaaaaatg
    18781 gtttaaagaa gaaggttatc ttcaaggaat gattcaattg ccagatgaat tgttccgtaa
    18841 taaacaatcg caaaaaagta ttttgatctt acagaaaaaa ggaccacaag cacaacaagt
    18901 aaaagaagtc ttgcttgtga aacttgcttc attaaaagaa ccagagaagg tgacggaatt
    18961 tttcaacgag tttaaaaact ggaaatcatc tgctctgtaa tcattcaacg agaaggagag
    19021 tcatatgtct aaaacaattg caatcaatgc tgggagttca agtttgaaat ggcaattata
    19081 tcaaatgcca gaagaaaccg tagttgcgaa agggatcgtc gaacgtatcg gtttacaaga
    19141 ttctattttt accatcaagt acggcgataa tcaaaaattc gaacaaatca tggatatcga
    19201 aaaccacgaa gtagcagtaa aaatgctgtt agatcagttg attgaactgc aaattttaag
    19261 ctcttatgat gaaatcaccg gagtagggca ccgtgttgtt cacggaggag aaacttatgg
    19321 ggattcagta gtgatcgatg agaaagtaat ggaccgcatt gctgaattag cagaatttgc
    19381 tcctttgcat aatcctgcaa acttaatggg gatcaaagcg tttaaaaaga ttttacctga
    19441 cattttgagt gtggctgtat tcgatacatc tttccactca acaatgccaa agcaaaatta
    19501 tttatatagc ttaccggtgt cttattatga agatttcaaa gtaagaaaat atggcttcca
    19561 tggcacaagt catcgttatg tgtcggaacg tgcggcaaaa ttattgaaca aaccgatcga
    19621 agaactaaaa atcatcactt gccacttagg aaatggtgta tcgatcacag cagtcgatgg
    19681 aggtaaatcc atggatactt ctatgggatt cacaccatta gctggggtga caatgggtac
    19741 acgttcaggt gatatcgatc cagctgttct cccatattta atggaaaaat tagagattga
    19801 tattgatgaa atgatcaatg ttttgaacaa gaaatctggt ctgctaggcc taacaggatt
    19861 gtctagtgat atgcgtgatc ttgaaaagaa ctatgaaaaa gaacatattc gtttagctta
    19921 tgacattttc gtggaccgca tccgaaaata catcggcggt tatgtgacaa cgatgaatgg
    19981 tgttgatgca attgtcttta ctgctggtat cggcgagaat gacggacatg tacgtagtga
    20041 aatcatcaaa ggtatgtctt ggtttggttg tgaaatcgat ccagcgttga atgaattgcg
    20101 tggtgaagaa gtagatatct ctacaaaaga atcaaaagta agagtattag tgatcccaac
    20161 agatgaggaa ctaatgattg cccgtgatgt ggagcgtctg cgcggctaag ccaaagcatt
    20221 cgttaggata aaagatgaaa cgcatgcaag agggaattct tgcatgtgtt ttttttgcgg
    20281 agaagaccgc aaaatggtaa tttaacttca atttcccttc ttttttacag gatcttctta
    20341 gggaattttg cttggaaaag tggtcaaaag ggaaaagact ggcattttat ttattgattt
    20401 tcaggaaaat cccaatattt tattttcatt catctttata agggacatta catatgtaaa
    20461 aagaaaaaac atttttagat cagcctcgtg ggctgtcgac tttattcttc gctttatgtt
    20521 tagctagaaa accgatccga aacattcatt gatcgaataa taaaaacttg attttccacc
    20581 tgtactttgc ctgaatcgtg aaagcacagg tatgaaaatc aagttttttg ttgcgaaaaa
    20641 acgtcctagt caatcagttg gccgagcggc tggcggctga tctgctcctg atcaataacg
    20701 agtgcttctt ggaagtgttg ctgcaaaaag tagcggtcat ggctgactgc taggagcgtt
    20761 ccttgatagt cttgcagcat ttcttcaatt tcttctcgag catagctgtc gaggtgattg
    20821 gttggttcat ccaaaatcag gacgttgatt ttttctaaaa agagtttcag caacgccaaa
    20881 cggatttttt caccaccgga taaatctttg atccgtttac ccacatcgct ttgataaaag
    20941 ccacttttgg ctaattgccg tcgagcgact tcttcgtttc cagtcaatgt gatggcgtaa
    21001 gccagcaaac ggctatcggg atcggcaaac tgcagctgct gcggcaaata accgatttgc
    21061 aagctactac ctaatttgat ggtgccaccg taaggctttt cctcaccgag taataatttg
    21121 agtaaggttg attttcctgc accattgtcg ccgatgatgg ctaaatgatc tccacgatag
    21181 actgcaaaac tgctgtcttt gaatagtact tgatcagcgt actgctggtg caagttttga
    21241 atgatgaaaa cttctttgcc agattttcca ccattagaca agctttgcag ccgatttttc
    21301 gggggaggtg gcggtttgac gagtgtcagt ttcgccaggc ggcgttccag ttctttggct
    21361 tttttgaaga aggactcatt gtctccttca tgtgcccact gacgaaaacg acgaatcatg
    21421 actttcaacc gctggacttc tttttgctgc agttcgtaat tttttgtcag ctcagccaag
    21481 cgagcttctt tcaagactcg aaaacggctg tagtttccgg gatactcaat caatgcacct
    21541 tcttccagtt cccatgtttt tgtggtgact tgatcgagaa attgccgatc atgagaaacg
    21601 accagaaccg atttttttgt attctgtaaa tagttttcca accagcgaat actgttaaga
    21661 tctaaatgat tggtcggttc atcgagtaaa agtaaatcgg catcggctgc taaaattttg
    21721 gctaaagcga ctttaactcg ttcgcctccg ctaagttctg ttaaccgtgc ttcctgacgg
    21781 tgtggaatcc ctaagccttg caagatcgct gcaatccggt cttctaatac gtaaccgcct
    21841 agttcttcaa agcgatgctg cagctcaccg taacgggcca gacacttctc taagtttgta
    21901 tctggttctg tcatcaatgc ttcgtagcga ctcatttgct gtttgaggtc ctgtatctca
    21961 gcggcagaat ggagcaagta gtggttgacg gtcgcttcgg aaactgtaag ttcttgttcg
    22021 accgtcccga tctttaagcc ttttttgcga ctgataacac cgctgtcgac accttctctg
    22081 ccggtcaaga tttgtaaaaa ggttgatttg ccagttccgt tgataccgac taaggcaatt
    22141 ttttcaccag cttcaatttg acaggataaa tcctcaaata atggattccc gccgattgtt
    22201 tttttaatag aaattgcttg tatgatcatg atctctctcc ttgagaccac tcaaaaaggc
    22261 catggttatc catggcctgt gttatttaaa ctcagattat catgagaaaa ataccttgag
    22321 atggccattg attttacgtg aattcaactg ttttggacaa acttgtcccg tcaacagcaa
    22381 ctgtaaaacc aattgcatgg ctaaatgcga tatggtatcg ttggtaattt tcattacttg
    22441 ttattttagt catgcgcatc atagtatttt ccctccttcg tgtagttgtc ttttccagtg
    22501 tacctgaaag tgtcagaaag ttcaactagc gctgacattt acttaatatt tatcgatgtg
    22561 taattctgtc agagagcttt ttttatctga ctgagggtaa tgtctcgtgg accacatcat
    22621 tgtattctga gctaaaccag cgttcaggcg aaatcatatg gtgtttgata aaaaactttg
    22681 tctacatgac ttccttttgc catttgctta ataccgttgc attaaacagc tcttctcttt
    22741 ggaaatctca ccaacatcgc gttcaggagt atcgttgtgg ttgcaatggc aagcggtagc
    22801 ctttcatcaa ttcttgaatg atttaccgaa tgtagcactt gccttctcgc ggtacccgaa
    22861 cccgaaataa aattttagtt tggcgataaa atcggtatcc gccatcgata cttcatattt
    22921 tttcgtatca gattcatcgg aaacttccac attgacgaac caataagctt tggcatgctt
    22981 aggtcgtttt aaaccacacc agggcgacta atcgccctgg tgttcacttt tttttatttt
    23041 acttgaactc attgactgtt ttccctttat tttaaacatt ataaagaccg atgagtgctt
    23101 gccgcgtctt ttgtttttat cacggttttt tgtgcaaaaa aacctgccga tagttggcag
    23161 gtcgtctacg tgttaagctt tggtgtggtc tttagctggc aattggtttt ctaaatcagt
    23221 tcggacaaca agcacatcgc aagctgcgtt tctaataaca tattcagaaa cagaaccaat
    23281 aaataaacgt tctacagcat tcaatcctgt ggctcctaac ataatcaaat ctacttgatt
    23341 atcttctggg atttctctgg caataatttg ttttggtgag ccatattcta cgacagaggt
    23401 tacattattc aagccagctt tcttcgcatt gctttcataa tctgccaatg tttgctttgc
    23461 catttcagta gcttgttctg caagcattcc atcaaaggat gaaacggttt ggaaagaacg
    23521 tgtgtcaatg acatgtgcta atagaagttc tccgttattt cggatagcta cattgaccgc
    23581 ctttttaaaa gctaattcag cttcatcaga accatctacg gcaaccatga tttttttgta
    23641 ttgttgtaac ataagcattc actcctcaag tcaatcgtat tgtactcttt tattgtagaa
    23701 cactttaggg aaaaagagaa ttatttactg ctttggttca gcaatttttt ttgcaaatcg
    23761 gatactgaag gtggcagaaa caataattac gatcagtaaa aagaccaagg caataaagcg
    23821 ttgattgaaa aaagctgtta aaatacccat tacgcctaaa acagcatatg cagctccgta
    23881 aaattgtagg aatccttgat ttttatcatt tttgttgatc aatacaaaaa agatatcctg
    23941 tttattaaat aaataatagc taacgaataa taatactaaa gtggcgataa tcattaaaat
    24001 cctaatcatg aacaaatcct ccaaaataag caacaaatag aaaaagcaat ttgccaagag
    24061 caaattgctt tattctttaa aagatggtaa tctccgttaa tgccgttgtt tgtcctgtag
    24121 tattgatccc gcgaattcaa gcaggtgggt cccatggtcg tagcttcgaa ctaccaaatg
    24181 ataaggcatg ttaccagcag tgacacaaga cgggtcccaa gatatcaata aaaatgttcg
    24241 gtcaacacac aaagaggaca attcgaacat cacagatttc ccacctctat agtagcatat
    24301 gaatttaaaa agagcaatga gagagctcgc agaaacagaa ccgttttctt gtctagtttt
    24361 ttggaggatg ctttttccac tcttttatac ggtaatactg ttgacccaga gcctgttcat
    24421 atttccctgt atcttttggc tgataatact gagcattctt cagtttatct ggtaaatatt
    24481 gctgattgac ccaagcctga tcaaaatgat gaggatactg ataacccacg ccacggttga
    24541 gtgatttcgc acctttgtaa tggctatctc tcaaatgatc aggaacatct ccagctttgc
    24601 cctctcgaat atcagcaagt gctgcatcta atgccatgta ggcagaattg gattttggcg
    24661 acaagcaaag atcgacgacg acgtctgcta gtgggatacg tgcctcaggc aatcccaatt
    24721 tttcagcagc taatacagcg ttcactgtac gagccgcagc agcaggatta cctaaaccta
    24781 tatcttcata accgataacc atcagacgcc ggcaaatact tgccagatca cctgcctcaa
    24841 ctaaacgggc gagataatga agtgcagcat ccacatcact tccacgaatc gatttttgaa
    24901 aagccgaaat aacgtcataa tgagcatctc catttttgtc atgggtaaga gcttttcttt
    24961 ggacacattc ttcaatgatc gacagtgtaa gatgaatcct tccttcttta tctgaaggag
    25021 tcgatagtgt agccagttcc agcccattca atgcactccg caaatcgccg ttcgttgcac
    25081 gcgaaaggtg aagaagagca tcttcgtcta actggattgc ttgttgtcca aggccgcgtt
    25141 ctttatccct taaagcatgt tcgactgcca actggatgtc ttgttctgtt aatggtttga
    25201 cttcaaagat ctgtgtacgg ctgcggatcg ctggattgat ggtaatataa ggattctcag
    25261 tagtcgctcc gatcaagatg attcggccac tttctaaatg aggaagcaga aaatcctgtt
    25321 ttgttttatc taatcggtgt acttcatcca ataagaggat cactgtacca ctcatttttg
    25381 cttcttccgc tacaacttgt agatcctttt ttgaatctgt agcggcgttt agcatgcgaa
    25441 atgcataatt agtagagccg gcgatcgcac ttgcgatact cgtttttcca gtaccaggag
    25501 gtccatatag aatcatggaa gagagcatgc gagcatcgac catgcggcgt atgatttttc
    25561 cttccccgac tagatgctgc tgaccaacga cttcatcgat cgttcgaggt ctcatgcgat
    25621 aagccaatgg ctgttgcata ggattttcct ccttcttgtt tttacgtccc ttaattatat
    25681 catatttagc aagatgccta gaaagaacag ccttctgttg ttttgttttc atagcttatc
    25741 caactaatat tgtgtatgat aaagtaggtg agaaaaatga cggataaaca aaagatagat
    25801 gaattttttt ggaataaaac gacgccagaa gtggcacggg atttattggg gatgtatttg
    25861 gagcatgaca caccagaagg aagattagct ggatatattg tagacgcgga agcataccta
    25921 ggtccagaag acgaggccgc ccatagctat ggtttaagaa gaacaccgcg tgtgcgggca
    25981 atgtatgaaa aaccaggtac gatctacctt tacacgatgc atactcatcg gattttgaac
    26041 atcattactc agccagaagg gattcctcaa ggagtaatga tacgtgcaat cgaacccgcc
    26101 gcaatgattg atcaaatgag taaaaatcgt ggcggaaaaa ctggtccaga tatcagcaac
    26161 ggtccgggaa aattagtgga agcattagca attcctcaag aactttatgg tcagtctata
    26221 gcagatagtt cgttacgctt agtttttgag aaaaaaaaga caccaaaaaa gatcaatgct
    26281 ttgcctagaa tcggtatccc aaacaaaggt gtatggacag aaaagccgtt gcgttttgtt
    26341 gtgtcgggaa atccttatat ctctttacaa agaaaaaacc aaatcgagaa agattggggt
    26401 tggaggaaag aaaatgaaaa agaaggatct attaacatat ttagatgaaa taattgaaaa
    26461 acaagtgaca gactatgatg tagctttaga ttgggataca aaaaatcata cgatcgagat
    26521 cgttgttcgc ttgtttgcag aaaataaagc acaatttgag attgatgatg cagaagggat
    26581 cgtttcgcaa gaaccaatta tcgaatttga agacggcgta cttcttttta atcctcagaa
    26641 atctgttttt gatgaacgca attatctagc agtaatccct tatgaaggaa aaaaagggtt
    26701 ggcaaaatca gtagctgatt ctttagtgga atatctaaat gaagtattag ctcaagggca
    26761 aagcgatttg cttgattttt tggatgaaga cgatgacgaa gctgtattcg agcttcactg
    26821 ggataatcag caactagcag aattagtaga agtaaaacag caaaatgaag cagatactta
    26881 tttaccgtac ccaagttatt aaggagtgac agatatgaaa tggaatgaag taaaagtaga
    26941 aactgaaagt gaagcagtcg aagcagttgc taatattttg atggaggcag gggcgagtgg
    27001 tgtcgcgatt gaagattctt tagatgttga acattttcag tcggatcaat atggtgagat
    27061 tttagataaa aatgatttta catcgctgaa agaaggcgca ctagtcatgg cttattttcc
    27121 ggaaacgact ttcttaccgg aaatcttgcc ttttatcaaa gatcgtatca cacaattacc
    27181 tgagtttggt ctagctattg gaaaaaataa agtaacagtt agtgaagtag aggaaagcaa
    27241 ctgggcaaca gcctggaaga aatactatca tccggtgcgt attacgcgaa tgctgacggt
    27301 tgcgccaagc tgggaaaact accagacaga agatcctcta gaaaaaatta tcacactaga
    27361 tcctggtatg gcatttggca caggcactca tcccacgact tccttgacat tgacagcact
    27421 agaaacagtc ttgcgtggtg gtgaaacatt gctagatgtg ggaactgggt cgggcgtgct
    27481 aagtattgca gcaaaatatc ttggtgcaaa agatgtctat gcctttgatt tagacgaagt
    27541 agccgttcgt tcggcgaaag aaaatatgga tatgaatgaa atagccaaag atgtccatgt
    27601 atcagctaac gatcttttga caggaatcga gattgaaagt gatgtaatcg ttgcgaacat
    27661 tttagcagat atcatcttat tgatgatccc agatgcttgg cgtttactga agcaaacagg
    27721 cacgctgatc gtttcaggaa tcattgaaga gaagaagcaa atggttctgg atgccatgtt
    27781 agaacaaggg tttatcgtgg atcaaatttt ccagcaaaaa gactggtatg caattatttt
    27841 gaaaaaacca gaggtggaat agatgcagcg gtatttttta aaagaatcat atgaatcaaa
    27901 agactatttt gttttagaag gggaaaacta tcaccacatt gtacgggtta tgaggatgga
    27961 acccgaggat gaagtttttt tggcattcaa tgatcaaata gccattgttg cagaaatcac
    28021 ggagattact gaagaggctg tttatctgaa agaaattaaa aaagaagaaa atcaaaagga
    28081 actgccaagt tcgattacca tagcgagtgg ctatcctaaa ggggaaaagc tggattggat
    28141 cgttcagaaa tccactgaat taggagcgca agcttttctt ggtttcccgg catcagcctc
    28201 tgtcgttaaa tgggatgcaa aaaaacgtat gaaacgacaa caacggcttg aaaagattgc
    28261 tcaagaggca gcggaacaat cccatcggca gatcgtgcca tcagtagagt tattggaaac
    28321 gaaacaagat ttatttgagc ggctagatga ttatgacgtg gtcttggttg cttatgagga
    28381 atcagcaaag caaggcgaaa aatcaaatgc agccaaaatt cttagtgaaa caaaaccaga
    28441 tagtcgaatc ttagctgtct ttggaccaga aggtggatta tcccgcgatg aagtcgatta
    28501 ttttgtagaa cacaaaggga tactttgtgg attaggacca agaatccttc gtacagaaac
    28561 agcacctctt tatttattaa gtgctgctag ctatcaatgg gaattaatga atccttcttt
    28621 cagctaacgt tcttcattaa taaaagagta tcgacagaaa gaattgaaaa aatagtcaac
    28681 aatttgtata atagaaacaa ttatttatac aatggatacg aaaaatcgtt tgaaaaagct
    28741 tttaaaaagc gaacatttcc gtaaaaaaac gtataactgc acggattttg ggacaattat
    28801 caaacagata tttgtcccga ttcccgtttt accgaaaaaa gaaaggggga tcgattcctg
    28861 tgacaaaaga agaaatttta acgggacccg gtgtcataaa gctcgttagt caatacatgg
    28921 gtcccgaaca tgtagcattt gttgagaaag catgcgaata cgcgactgct gcacatgacg
    28981 gacagtttag aaaatcaggc gaaccctaca ttattcatcc tatccaagtc gcaggtatat
    29041 tagcagattt aaaaatggat ccccatacag tggctacagg cttcttacat gatgttgttg
    29101 aagatacaga aatcacttta gaagatctga gagaagaatt tggcgatgac gttgctatgt
    29161 tagtggacgg cgtaaccaaa ttagggaaaa taaaatataa atcccacgaa gagcagctag
    29221 cagaaaacca ccgaaagatg ctgctagcaa tggctcaaga tttacgagtt atcatggtca
    29281 aattagctga tagactacat aacatgcgta cgttgaagca cctgcgagaa gataagcaaa
    29341 gaaggatcgc tcaggaaact ttagaaatct atgcgcctct tgcacatcgt ctagggatca
    29401 gccggatcaa atgggaatta gaagatacag cacttcgtta tctaaatcca aaacagtatt
    29461 accgtatcgt ccacttgatg cagacgaaga gagaagaacg tgaaaaatac gtaagtggta
    29521 ctgttgaaga tattcgaata gcgacagaag agctggggat ttttgcagaa atctatggac
    29581 ggccaaaaca catttattca atctatcgta aaatgaaaga tcagaaaaag cagttcaacg
    29641 aaatttatga cttgctagct attcgagtga tcgtagattc aatcaaggat tgttatgctg
    29701 tattaggagc aatccacaca aaatggaaac caatgcccgg cagatttaag gattatattg
    29761 ccatgccaaa agctaatatg taccaatctt tgcatactac cgtgatcggt ccggcaggaa
    29821 atccggtaga aattcaaatc cgaacacagg aaatgcatga aatcgctgaa ttcggggttg
    29881 ctgcacactg ggcctataaa gaaggaaaaa atgaaaaagt agaaccagat ggtatgacga
    29941 aacaattaag ctggttccat gagatactcg aacttcaaga cgaaagctat gatgcttctg
    30001 aatttatgga aggcgtaaaa ggagatatct ttagtgataa agtctacgtc ttcacaccaa
    30061 aaggagacgt tactgagtta ccaaaaggat ccggaccatt ggactttgca tacagtatcc
    30121 atacagatat cggtaacaaa accactggtg caaaagtaaa tggcaaaatg gtgcagcttg
    30181 attacaaatt gaaaaacgga gatatcattg agatcatgac ttctccaaat tcatttggcc
    30241 caagtcgcga ctggttgaaa ttagttgcta ctagcaaagc aagaaataag atcaaacgtt
    30301 tcttcaaagc ccaagatcga gaagaaaatg tgatcaaagg ccacgaatcc gtggtcaaat
    30361 gtattacaga tctaggattt acgcctaaag atattttgac gaagaacaaa ctgcaagaag
    30421 cactcgatcg ttttaattat caaacagaag atgatctcta tgcagctgta gggtatggag
    30481 aagttagccc cttgacgatg gccaatcgtc tgactgaaaa agaacgtaaa gaacaaaaaa
    30541 tcgagcagca aaagcaagaa gcagaagaaa tcatgaatca gccgaaaaaa gaacctgaca
    30601 aaatgaaagt acgtcatgaa ggtggcgttg tcattcaagg ggtagaaaac ttactaattc
    30661 gtatcagtcg ctgctgtaat ccgattcctg gtgatgatat cgttggttat atcactaaag
    30721 gcagagggat atccattcat cgtcgagatt gtccgaatgt tcagcctgac aaaccaaatg
    30781 tagcagaacg tttgattgaa gtcgaatggg aagatacatc gaatacacga aaagagtatg
    30841 atgcagattt ggaaatttac ggctataatc gttcaggctt attgaatgat gtacttcaaa
    30901 cagtcaatgc gctaacgaaa aatctcaaca gcgttgaagc acggacgaat aaagataaaa
    30961 tggcgacgat ccatttgacg gttggtatcc agaatttatc ccacctaaag agtatcgtgg
    31021 ataaaatcaa agcagtacct gatgtctaca gtgtacgccg gacgaatgga taggaggaca
    31081 gatatgagag cagttgttca acgggtaagt aaagctgctg tgtcagtcga tcagcaagaa
    31141 attggaaaaa ttggctacgg cctacttgtt ttattaggca ttcatgaaac cgatacgcaa
    31201 aaagatgtgg actatctggt aaaaaaaatt gcacaaatgc gtatttttga agatgaacaa
    31261 ggaaaaatga atattagtgt agaagatgtg ggaggacaaa ttttaagtat ctctcaattc
    31321 acactgtttg cggatacgaa aaaaggaaat cgaccaagct ttgtggaagc cgcacgacca
    31381 gaaacagcta ttcctttata cgaatcattc aatgaaggaa tcagaagtcg cggcatcaca
    31441 gtcgctaccg gacaattcgg agcagatatg tcggtttctc tcgtcaatga tggacctgta
    31501 acgatcatta ttgattctaa aaacaaataa agtaatctgg ttttcactag taaaaactat
    31561 gaaacacgtc gcttaagtta gactgataaa ctctaactta agtggctttt ttgtctgcaa
    31621 aaagcgcttc ttttgtcagc agtcggaaaa aaagttaagc ttaaattata caagttattt
    31681 tttataggag gttgctgaag atgaaagaag taccagtagt agatatcaaa atcacagatt
    31741 taaaaaaatt gtatcaaaaa ggatacaaca tgttggagga gttacgccat gaagcggacg
    31801 ctcccgtcgt caaagcaaag attttcaata aagaagctat cacgatctac ggatcatcag
    31861 cagcaaaagt attttatgat ccaaggaatt tcaaacgaaa aggtgctatg ccaaaattag
    31921 tattgaagac actttttggt caaggtggag tacagacttt ggatggagct gctcatcacc
    31981 atcggaaaaa tatttttatg gatctaatga caccggaaag aatggaagat tatcatcgta
    32041 tcttagataa gaatctgaca caagcacttg aagcacagca tggtcaattt gaactatttg
    32101 atttaagtaa gatggttttc tttacatcta tttgcgagtg ggcaggtatc aatttatcag
    32161 cgatttctaa agatgaggtc gaaaaattag cagaatatca aatctctatg atcagcggaa
    32221 cgtttacttc tccgattgat cacataaaag gtgtcgaaaa tcgcaaaaaa tcagaaaaat
    32281 gggcgcaagg cttgattgaa gaagctcgac aaaatcctgt ggctggaaaa gaaaatgtgg
    32341 cattatatgc ttttgccaat gccacagatt tagatggtca gcttttacct ctggaagtgg
    32401 cagctgttga attattgaac atcatccgtc caacggttgc tctaactgtg tgggcggcat
    32461 tgatgggaca tgcacttttc agtagaccag atttgtatca gcaattaaaa aatgactttt
    32521 cgacgttaca agatccgttt attcaagaaa tgagacgcta ttatcctttc ttcccaatgc
    32581 tacctgcgat ttcgctgaaa gaagtagaag tagatggtta tcgtatacca gaaggcagtt
    32641 gggttatttt agatttatat ggaacagacc atgatgaacg aacagttgaa gcgccagatt
    32701 cctttatgat caaacggtat gtagggaaag caaaagatat ttcctataaa gaagaatatg
    32761 agatgattgc acaaggcggg ggaaattttc gacaaatgca tcgctgtgca ggagaatgga
    32821 tcactcttca tagtctacgt gtgttttccg atcagctagt aaataagttt gagttctctg
    32881 tacctgaaca agattggacg attccattca atcagtttcc gacttatccg aatagccgag
    32941 cgctacttta caagaattag attagcataa acttatcgaa aaaaaggaag atcaaacagt
    33001 gctgatcttc cttttttcat attactataa attgataagg tcaattgatc aaaatccacg
    33061 aaatgcttca tcaatggttt gataatcttc attggataaa tgcaaatcta aagcttggac
    33121 attgtcagat acttgctctg gacgcttagc tccaggtata actacagaca ctcgtggatt
    33181 cttcatgtac caagctaaaa caagttgcgc aggagtggct tggtatcttt tagcaatggg
    33241 gcgaagtaca tcgacagcgg ttaaagtttc tctgaaacgt tctccttgga aatctggatt
    33301 tctttttcga ggatctcctt caccaaattg tttttcctca ccaagttcat attttcctgt
    33361 cagcaaaccg gaagcaagag gaaaataggg aacaaaactg attttgtttt tttcaagata
    33421 agggaataat tctttttcag cctgccgatg gatcaaactg tatttatctt ctactacatc
    33481 gacatatcca tctgcattcg cttctttcaa ttgttctaac gtgaagtttg acacaccgac
    33541 tgctcggatt tttcctgctt cttttaattc gtgcaatgta gcaactgatt cattttttgg
    33601 tgtgctttca tccggaaagt ggatataaaa aatgtcgatg taatcagttt gcagacgttt
    33661 tagtgcatct tccaccgatt gtttgagaaa ctcaggagaa ttatcaaatg ttcgcccttt
    33721 atttggtaca tgagcagctt ttgtagcgat gacgacacgt gagcgatcat attctttttc
    33781 cttcagtacc tcgccaatca gttcttcgga acggccgttt ccgtaagcat aagcggtatc
    33841 gatcaaattg atcccgctat tcaaagctgt acgaactact tgttttcctg tctcatcttc
    33901 caaaccgtca aaaagattat gtcctccaac ggcatttgct cctaatccaa gctgttctgc
    33961 gtagacttgt gaatgtccga ttcttatttc tgccatagct gattcctcct ttttgtctag
    34021 tctaaaacaa aataccaaaa agctgaacta aaacgttttt gtgaggggaa atagtcagat
    34081 atctcttgac tttgagactg gtttttagta ggttataagt aatatcgaat gtcaatgaaa
    34141 gagcgagtaa gtttttgaaa ctttacaaga gaggatcatc gaggctgaaa atgatctaaa
    34201 gtttcattag cctaaagaca ctctggagat ggacgagtga aaagccgtcc cgcgtacgag
    34261 cgttattcgt aagaggaaag agaaatcttt caaaattagg tggtaccgtg tatgaatttc
    34321 attcaccctt gttcatgatg aacaagggtg atttttttat ttacatagct aagaaaggga
    34381 gagaaaacta tgagtttcca aagaccaaaa ggaacaaatg atattttgcc aggcgaatca
    34441 gaaaaatggc agtttgtcga agaaacagca cgtttattat tcagagatta tcagtataat
    34501 gaaatgcgta caccgatttt tgaacactat gaagtgatat cccgaagtgt aggggataca
    34561 acggatatcg tttcaaaaga gatgtatgat ttttatgaca aaggggaacg ccatgtgacg
    34621 cttcgaccag aaggtacagc accaatcgtt cgttcttttg ttgagcataa attgttcggc
    34681 ccagagtatg cgaaaccgtt caaaactttt tatatgggac caatgtttcg ctacgaacgc
    34741 cctcaaaaag gacggttacg tcaattccat caaatcggag tagaagcatt tggatcagaa
    34801 aaccctgcaa ctgatgtgga gagcatggtg atggcacttg atttcttcaa acagttgggg
    34861 atcacgcaaa tccggctagt catcaattca ttaggtgata aagaaacgcg tcaagcctat
    34921 cgtcaagcat tgatcgacta tcttttacct tttgaatcag agctgagtga agactctaaa
    34981 cgccgccttc atgaaaatcc attacgcgta ttggacagta aagacaaacg agatcaaaaa
    35041 tttgtggcag aagctccatc tatcttagat tatctaagcg agcctgctca aaaacatttt
    35101 gataccgttg tttccatgct ggatgcattg gctgttcctt atgaaatcga cagtaatatg
    35161 gttcgcggac tagattatta cacacatacg atttttgaaa tcatgagtga cgcaccaaaa
    35221 atgggggcac aagcaacgat ttgcgcaggc ggacgttatg acaatctagt ggaagaacta
    35281 ggtggtcctt caacacctgg cttcggattt gcaatgggta tcgagcgatt actcttgact
    35341 atggaggcag aggaagtagt tatccctgca tttaatgagt tggatgctta cgtggtcgca
    35401 ctcggagacg aaacaaatat cgaagcattg aaagtggtcc aagcgattcg aaactttggt
    35461 ttttcagctg accgcgactt catgaaccgt aaagcaaaag cgcaatttaa gacagcagat
    35521 aaagcgaatg ccaaattagt attggtgatc ggtgaagatg agcttgctag tcaaacgatt
    35581 aatgtcaaag caatggcaag ccgaaaagaa aaaagttttc cattagaaga gatatatgaa
    35641 cattttgatg aagtctatga tgaaatgact ttattcgagt aggaggaaaa aaataaatgg
    35701 cacaaagaac gatctattgc ggattagttt ccgaacaaca attagaacaa acagttacat
    35761 taaaaggatg ggtacaaaaa agacgagacc ttgggggagt aatctttatc gacctacgtg
    35821 atcgtgaagg aatcgttcaa atcgtcttta atcctgaaat caacaaagaa gcatgggaaa
    35881 tcgcggataa atgtcgcagt gaatacgtga tcgaagtgac tggggaagtt agaagacgcg
    35941 gagaaaatgc agtaaatcct aaaatggcca ctggtgaatt cgaagtgatt gcaagtgaaa
    36001 tcgtgatttt gaatcaagca aaaaccccgc catttcaaat tgaagatgat caagcaatca
    36061 gtgacgaaat acgtttgaaa tatcgttatt tggatctgcg tcgtccaaaa atgactaaaa
    36121 acatcaaatt aagaaatgcg acaacaaaag cgattcgtca ttatttagat gataatgact
    36181 ttctcgatat cgaaacacct tacctaggga aatctacacc agaaggcgca agagactacc
    36241 tagttccttc acgtgtccat gctggacatt tctatgcgtt gccgcaatct cctcaattgt
    36301 ttaaacaatt gctgatgaat gctggattcg accgttatta tcaaatcgtt cgttgtttcc
    36361 gcgatgaaga tttacgtgga gaccgtcagc ctgaatttac gcaagtagat attgaaacaa
    36421 cgttcttaac agcagaagaa atccaaactt acacagaggg attgattgcg aaggtaatga
    36481 aagaagttcg cggtatcgaa gtcacattgc catttcctcg tatgacctat gatgaagcaa
    36541 tggctcgcta cggcagcgac aaaccggata cacgttttga tatggaattg atcgatctaa
    36601 gcgatacagt caaagaagta gaattcaaag tcttccaaat ggctcttgaa aacggcggtg
    36661 tcgtgaaagc attgaatgct aaaggcgctg cagatcgcta ctcacgaaaa gacatggatc
    36721 agttaggtca atatgtagga caattcggag ctaaggggct tgcatggctg aaagtagaag
    36781 aagacggatt gaaaggtcca attgctaaat tcatgggaga agcaacagaa gcaatcatta
    36841 aagcaacaga tgcaaaacca ggtgacttgc tgatgtttgg cgcagataaa agtgaaatcg
    36901 tcgctgctgc attaggtgct atccggacac gtttaggaaa agaattagga ctaattgatg
    36961 aatcgaaatt caatttctta tgggtaactg attggccaca atttgaattt tcagaagaag
    37021 aaggtcgata tgtctctgct caccatccat tcacgatgcc aaaagaagaa gatatcccgt
    37081 tattggctga agaccctgca aaagtctatg cagaagctta tgatatcgtc ttgaacggtt
    37141 atgaactagg cggaggttct atccgtattc atactcgtga gcttcaagaa aaagtactag
    37201 aaactctagg ctttacaaaa gaacaaatgg aagaacaatt tggtttcttg ttaacagctt
    37261 tagattacgg attccctcca catggcggta ttgcattagg attggaccgt tttgcaatgt
    37321 tattagccgg agaagataat attcgtgaag tcattgcctt tcctaaaaac ggaaaagcag
    37381 cagatgtgat gagtgatgca ccaagtatgg tttcgccttt acaattgttc gaattgaata
    37441 ttgatgtaac gaatatagaa gaatagccga taaaaaggta aaaaggattt gtcgaatacg
    37501 cttcttttga caaccttgat ttaattgaaa aacactttat aatgaagcca taacatataa
    37561 agcggggctt ttccatgaaa aaaacattcg aaaattgggc ttatcatgat tacacgacca
    37621 agctttcggt atctatcgtt tatgcgttga tcgcatcagt cgcattgaat ttcttttatc
    37681 agccaggaca tatttattca agtggtatta ctggtctagc tcagattttg acgactttgt
    37741 caaaatcact gataggattc gaagtaccgg tatcaacaac tttgtattta ctgaatatac
    37801 ctttgtttgt attggcgtgg ttaaaaatcg ggaagaagtt tacaattttc acttttatca
    37861 cagtcacttt gacctccctt tgtatccata ttttaccgga aacagtccta tctaccgatc
    37921 caatcatctg tgcgttgttt gggggggctg tgatgggact aggtattggg ctagcgttga
    37981 gaaacggcct atcatcgggc ggtctagata tcgtgagtat cacgattcgt aaaaaaacag
    38041 gaaagtcgat tggttctatt tcgatctact tcaatgcatt gatcgttttt gttgccggaa
    38101 ttctatttgg ctggcaatat atgttttaca gtacgttgtc tattttcgtc agtggaaaag
    38161 taactgatgt cgtttatact aaacaaaaga agatgcaggt aatgatcgta acgaaacatc
    38221 cagaaaaagt agtaaatgaa atccaaaact gtatgcgtag agggatcacg atcatcaatg
    38281 aagcagaagg tgcttataag catgataagc agacagtatt gatgacgatc gttacacgat
    38341 acgaactgcc tctacttaga gaagcaatga aagcttctga tccacaagct tttgtaagta
    38401 ttgcagaaaa cgtccagata cttggtcgtt tctatgagga tggcatttag atcgagtttg
    38461 acaaaaattc atgaaatact gctgtagaca gacgttcgtt tgtctgcagt ttttttctac
    38521 catagtagaa tcagcaaaga aaaactgctt ttttatttga aattttctta aagttgaaaa
    38581 aaagcatgtt tattttttta aatttatggg atgatagaaa aaaatagtag agaaaggaca
    38641 cgattctact tgctattttc acttaatacc cgtatattac gaatgaaaaa agtttatgaa
    38701 aagttggaat attgaatgga aaaacaaaaa aggttaaaaa acagccgcta catcactggg
    38761 tttgatgggc tccgttcatt agctgtggtg ggtgttatct tctatcacct tctgccaact
    38821 agtatgaaag gcggctattt gggcgttccg atttttttcg tcgtatctgg ttatttgatc
    38881 acggatctgc tgcggcagga atgggaacaa aatggaaaaa tcaatatttg gcaattttat
    38941 gtcagaagaa tgaagagact ctatccggca cttgttttta tgcttgtgac agcttcggct
    39001 tatatcactt tgtttcagcg agggctgtta aacaatcttc gaggagttgt ggtcagcagc
    39061 ttactgtatg tcaacaactg gtggcaaatc aataacggtt tatcttattt cgatcgattt
    39121 gctaatgagt caccgtttac gcatatctgg tctttagcag tagaaggtca aaactatttg
    39181 gtttggccga tcttgtttgt attgttgatg gtatttgttc gtaaaagaaa atggatcttt
    39241 tatactgtgt taggtacatc cttcctctcc gccatcttga tgatgatctt gtatacacca
    39301 ggaggcgatc caacccgtgt ttattacggt accgacactc gtctgttttc tatatggttg
    39361 ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa agaaaaacat cccgaaacaa
    39421 gcgaaaaata ttctgaatat cgctggagga atctcgcttt tggcactgat tctttgcttc
    39481 ttctttttag atgatcatta caatttcatc tattacggag gaatgctcct agtgagtctg
    39541 ttatgcgtag tgctagttgc agtgacagca caccccggag caagtttgaa tcgatggctg
    39601 acaaatccag tatttacttg gattggcaaa cgaagctatg gcatttatct gtatcagttt
    39661 ccagttatga ttttttatga agcaaaggtg actgatattg ccgatcatgt tttgttgcat
    39721 actttagtgg aaatcacatt gattctaatc atcagtgaac tttcttatcg atatgtggag
    39781 cgtccgctag cacgttttga ttatggtcgt acattccaag tgatgaaagg atggttcagc
    39841 aaaccgatca tcagtaagag caaaccatgg gttgttccag gcacattact tgtagcaatt
    39901 gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa cagccgatca aaaacaactg
    39961 caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa ccagaaacaa ttcagaaaac
    40021 accactagtt cggttgacca agcgatcctt gataagtacg agttgtccaa ggcacaaggg
    40081 aataaggcac aaaaattaga actaacggca tttggtgatt cagttatgtt ggatgctgca
    40141 acggatctac aagaacttta tccaaaagta gtggtagatg gagatgtcgg acgacaatta
    40201 tatgccagtg taccgtacat tgaaaaatta aaggaacaag atttgttgaa agacactgtt
    40261 ctgatcggac taggaacgaa tggctcattc tcggaagctc agtttgatag tgtgatggat
    40321 gcaatcggtg atcgaaaagt atactgggtc aatgtccgag taccgacgaa gcgctggcaa
    40381 aatgacgtga atgcgatgct tgaacagatg acgaaaaaat acgacaatat gacgctgatt
    40441 gattggtaca gctacagtaa tgaccatagt gactggtttt atgatgatca agtccatcca
    40501 aatcctgaag ggatgacaca ttacattcac ttagtcagcg aagccttgct aggaggaagt
    40561 tcaagtacga acagcagtga gacaaacgtg acagaatgat agctacaaaa aacttcaggc
    40621 aaaaacattt gtttttgcct gaagtttttt tatatttacc gtgtttttaa cattcatata
    40681 gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac gttctttttg ttttcgggtg
    40741 agttttttga atgcagcttc tgcttttgtt gcttcgcttc ttgaagaaaa tacttctgca
    40801 tggatcatct taacaggtct tcttttttga agacgagtat atttagcacc agtaccattg
    40861 ttatgttcct ctaatcgtct agaaagatcg gttgtatatc caccataaaa tgtctgatct
    40921 ttacagaata atacgtaaaa ataatgttca tttgccatag agcatctcac gaatctctgc
    40981 agtgtattct ccatccatat tgtaagtgat caatggtgga gcgactttga atccgtctaa
    41041 tttgccttct ttgattcctt caactaacaa aatatttgcc tcttttccag gttttgggta
    41101 aacaaattgg atccttttag gtgcaattcg atagtcttcc atgacctgaa tgatttctaa
    41161 gaaccgatca ggacgatgga ccatagtaaa gcgtccattt gtttttagta gcttacttga
    41221 aatatcgatc acttcttcta acgtcgtatg aatctcatga cgggcgattg ccaggtgttt
    41281 attagggttc gttttatttg ttggcagccc tttgaaataa ggtggattac agacgactaa
    41341 gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc agatcgatgg tgtggacagt
    41401 catttgttca tttaaatggt taagcgcgat actacgtctt gccatgtctg ctagacgagg
    41461 ttgaagttcg atagcgtcga tagccgcctg ggttcgttta cttaaaaaaa gtccaactgc
    41521 gccatttcct gcacaaaggt caacaatctt ccctttttta ggtacacggg aaaagtgagc
    41581 aagcagcaca gcatctaaag aaaaagaaaa cactttggaa ctttggataa tcttgatatc
    41641 ttctgcgtat aattggtcga ttcgttcgtc ttcgtgaagc atggattccc tccaaaatga
    41701 aaaaaatagt cgtctgtatt atactatttt tattaaaaag aaccaaggtg gcttgtcaaa
    41761 cctctatttt ttatgcatac tatgaccata agaagggaaa ggaagagact atgttttatc
    41821 gatttatgcg cggggtagtt cgagtactat tagccttagt taatggaaat gcccgctatg
    41881 agaataaaga agttttaccg caagatgaga attacatact agttgctccc catcgtactt
    41941 ggtgggaccc attatatctg gcggtagctg cagcacccaa ggaattcagc tttatggcaa
    42001 aagaagaact gttcaaaaat ccagttctgc gatttatatt aactcgatcc aatgcttttc
    42061 cagttaaaag agaaaatcca ggaccaagcg tcattaaaac acctgtaaag attttgaaat
    42121 cgacaaattt gagtttgatc atgtttccca gtgggacaag acatgctaca gaacttaaag
    42181 gcggaatggc attgatttcc agaatgtcta aagcgaaaat cgtgccagct gtatatcaag
    42241 gtccgttgac acttggggat ctgttcaagc gtcagagagt gacagtgcgt ttcggcgaac
    42301 caatcgactt atcagatatc aagaaaacgg ataaagaagg tatggcggag atcgaacgac
    42361 gaacacaagc agcttttgac aaattggaca aagaagtgaa ccctgatttc aaatatgaag
    42421 tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc ttttgaatct actgtcctgt
    42481 tcagttcaga ctttttcaat gaaaacgagg gagagacgtc aagtctgccc tctttttgcg
    42541 ttccaatatg taaaatagaa gacaggtgtc atgtaatgga tcaaagtgtt ataataatta
    42601 gaaggacatt ttttgaggtg attgaatgcg ttttttacat acagccgatt ggcatatcgg
    42661 aaaaaaacta caaggttatg acttgattga agaccagcgt catgtactat cttctatact
    42721 agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt gccggagact tatatgatag
    42781 gagtgtccca gctgttgatg ccgtagaatt atacaatgaa ttgatggtcg attggaattt
    42841 aaaggaaaag atcccgattt ttgctatatc aggaaatcac gatagcagta cgagactttc
    42901 agcgggtacg ccatggttca gttattcgaa ctattttttg cgaacacagc tagcagaagc
    42961 atttgaacca gtcgagttta aaaacgtaca atttttcttt cttccttact ttgaaccaat
    43021 tgcagcgaga ttatattttg aagatgacaa tatccgaacg atcaagcaag cggtagccaa
    43081 ggtgatagat aaaatgcagg agctatttga ttcagaaaaa aaacaagtat tgattagcca
    43141 tttttttgta atgggaagtg taaaatctga ttcggaaacg aaaatcgaag taggcgggtt
    43201 ggattcgatc cctggtgaac tacttacacc gtttgattat gtagcactag gacatttgca
    43261 taatcaagct gctttgagac aggataatgc tcgctatagc ggttccccat tgaaattctc
    43321 tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt gacctgacaa caggcctgga
    43381 gttgaatttt agggagatcg aacccctgca tgatattgaa gaaataacag cgagtttcaa
    43441 agaattgctg gatcctgcct tttatcagtc cgtcaatcgg gagaattaca tctatatcta
    43501 tctcactgat agaacgatca tcccgaatat gatgaaccag cttcggcaaa tatatcctcg
    43561 tattttgggg attgaacggg caaacggtcg agcagaacaa acaaagcgca aacgaaaagt
    43621 actagaaatg aaagatcctc aaaaattagc agaagcattt tttagtgaaa tgacggaaga
    43681 gcagctaact gcaaaccaag aaaaatggct cggagaaacg ttgcaagaaa tcatggaaat
    43741 ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa aattttggtc cttttatcaa
    43801 tgagaccgta gatttttcac gtttgacaga agcaccgctt tttttgatca gcgggaagac
    43861 aggagctggc aaaacgacga tttttgatgg gatcaccttc gcactttttg gtgaaacttc
    43921 cggaagactt cgctctggaa aagagatgcg ttctttgttt gctacaccag acgaagaaac
    43981 aagcgtgact ttttcttttg aacatcagca gatgagctat gaaatcgaac gaaaaccaga
    44041 acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa caggcagcca aagtaacgtt
    44101 gaccattttt gatgggaaag gaaaagaaat aaaacaaatc acaaaacgta cagaagcaga
    44161 tcagttgatc agagaactga tgaatctcga tgcgaaacag ttttctcaaa tcgttttact
    44221 accccaagga gaattccgta attttttggt ttcatcaagc agtgataaag agactgtttt
    44281 acgtcattta ttcggtactc aatttttcca acagttcaat gagcgattaa aagaaaaagc
    44341 aaaacaacag caacaaaaat tggatcattt agagcaagag ctgcatcttc tgcaaaaaag
    44401 gtttgtttta gtcaaagaag aggaaccagc agcagggttt gaagccgttt tagcccaatg
    44461 gacaaaccat caggcaatac tcgaaaaaca aatcacggaa gaaaaagaag ctctaacgga
    44521 acttcaggaa aaacaaaagc aactagaaaa ttcctactat cgttttgaaa gcttgaaaaa
    44581 aagttatgaa gaaaaaacga tattaataaa caagcaaaag gaattagctg ctcaaaaaga
    44641 cgaaatagag gaaaagaaac gatggattgg gtattatgaa tttacgcaac gattagtcga
    44701 accaatccag cgcctacaag aaataaaagt tgaaaaaata gaactgtcac aaaaaagtag
    44761 tgagcaaaca aagaaactag cgatgactac gcagaattat gaagagtggc aaaaaaatga
    44821 agcaaaccgc gtacgccgac aaacaaaaat caaagaagct gctcaaaaac tgcaaaacaa
    44881 acagatgatg gtgccaattg tcgaatcctt acgtagtaaa aaacaagacg cagaacaatt
    44941 aacaatttca ttggataaag aagaattgaa tgccgagaag ctgtatcaaa atctcgctat
    45001 ctatcaggaa agaactcgaa ttcttcaaga aaatttactg gatcaggaaa aactgcaaga
    45061 agatcgtctt gcttttggac agttaaaata tctagatgaa aagcagcaag aaatagaaaa
    45121 acaaaaagca aaagacactg ggtcgctcgc acagataaga gaagaactca aaaaagcaac
    45181 tgaagaaata acaaaaaata gtcagctatt gaaagaacag acagaacaag cagaacagct
    45241 gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag ctttttttgc gtccagggga
    45301 gccatgtctt gtttgtggat caacggaaca ccccatacaa caattaaaac acgaacagcc
    45361 aaataaggaa gatatcttaa aagtagaaca acaactggca gaagctgagt ctgctgtaga
    45421 aagaactcag caaatcattg ccaaaagtga agcacatgaa aataatcttc aacaacaatt
    45481 atcagattta gaacaaaaaa tcatggcaac gaatatacaa ttggctgaac aagaagaaca
    45541 cttgcaacag gctttgaaaa aggcatttcc tgagataaca gaacaaaaaa caaaaacaac
    45601 tcttgtagta atcgaacaat ctttagatag acgaaaaagc aagatggcca atgcaagaat
    45661 agagctggaa gaaataagac aacaagcaga aaagctcgaa caacaattag ctgtctacaa
    45721 acagcagcag acagaaaaaa ataatcagct ccagcaactg caaggagaaa tcaaaagttt
    45781 agaacaacaa ttgcaagata cggatagtaa ctgtttagaa gaggagatac gacgacttga
    45841 aaaacagtta gaagaagact cgaatcaact agctgttgag cagaaagcag gagtgcaatt
    45901 gttgcaagat cttcatacgc ttcggacaca gcttgatctt caacatgaac aaatagaaaa
    45961 aatagatgaa aaaatagaaa aggaacaaga aagaatagct aaaaagttag aaagccagtc
    46021 gtatttttca acaattgaag aaatctgtgc atttgcacaa cagcaagcac aatataaaga
    46081 aaaccaacgt acagttactt cttatcatga agagcaatta gtgacagctg accggctgcg
    46141 tcaattagaa aatatccagc taactgagac attcccagat acagaagaga tcagccaaca
    46201 gttagaagaa ataagacgta tgatcgaaat aaagcaagaa aagatatacg gtttacaaga
    46261 acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt tcgatttatc acacaagcca
    46321 gacgaaatta gatgagctta cgcaattaca gcagctttcg caaacattca acggagaaaa
    46381 tcctaagaag actagtttgg aacggtatgt gcttcaggtt tatcttcaag aagtgttaca
    46441 agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt tatcaatttg agctagcgga
    46501 taccattggc agttatcgag ggaaaaccgg attagaaatc aatatctatg atgatcaggc
    46561 aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag agttttattg cagcactttc
    46621 tttagcatta gctcttgccg atgtgatcca gacacaagct ggtggtgtag caatcgatgc
    46681 attgtttatt gatgaaggat ttgggtcatt agatgaagaa gctttggaaa tggcaatgga
    46741 agctttagaa acgatcgaaa gtgaaggaag aatgatcggc atcattagtc atgtaagaga
    46801 attgaaagaa cgagtattac aacaaataag aattgataca gaaggtagcg gacaaagcaa
    46861 aatacgctat caacttggat aaaaagggag attggtaact atgaaatgga gtattccaga
    46921 aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc cgagtgttat cggttgtccc
    46981 tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc agtgaattgt atatggttga
    47041 tttggatggt acagcaaaag agatagatta ctgccaatgt ccctattggg aggaacataa
    47101 atattgtaag catacagttg ctgtggaact ttatttacgg tcgaaaaatc tatctcgtat
    47161 catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca acaagtgagg gagaaatcat
    47221 tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct caagcggtgc aacctttgat
    47281 tattgaatac caagtagaaa cgattgagac taatcagtat cacccagagc tttctatttt
    47341 ggcactttac ttgcgtatcg gttcattggc atcaccgaaa aaaacatata tcgtaaaaaa
    47401 catctatgat tttttacaag cttacacaga aaaagaaact tacactgtta ataaacagta
    47461 ccagtttcgt ctagataaac gggcgtttca aaaagaagat caaaaaatac tcacgcatct
    47521 tgcaggaagt gctcagacgc atcaattatt aggtacgaac ggcttacaag tcaaaggaaa
    47581 actagataaa agatatctgc ttctcccagt agaacaagcc cgcttcctgt tagagcagat
    47641 gaatcagact acacgtttgc atctgcagat aaatgaacaa aacgatcaag gaatacaatt
    47701 ttccgatcaa aacaaacctt tatcattcaa agtggaaaaa tttggagaag attatttgct
    47761 gcaagcattg aatgattttg attttttctt tatgcattat cagtggggag ttatccaagg
    47821 gacattttat tctctgacga aatatcaaca aacggtttat caaacttgga aacaattgat
    47881 tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa gaattaccgt ctttgtttaa
    47941 agagattatc cctctgctgt cagagatcgg cgacgtagct attgcccctg cagtagaaga
    48001 ggaagtagca gatatccccg tcgaatttat tttctttttc cgaaaagcaa aaggaaaaat
    48061 ccaagctcga gtcgatttcg tctatggcga agtcatctat tcgactgacc cgaagcatga
    48121 ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca gtagaagaga aacgagtact
    48181 tgatctattc agaatgtatc gctaccagaa aaatgaaact ggttatgaac gtccattgcc
    48241 aagcggggaa gaactatatg cctttttccg cacagaacta tctgtcttcc gtcagtttgg
    48301 tgaagtcaga cttggaagaa aattgcgaga gttgtattta gatgcgcata aacatcaacc
    48361 gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt cgattcgatg tgacagggat
    48421 agaggagcaa gaaatcgatc atgtcctgca aagcttgctg agaaatgatg ctttttatac
    48481 attagaaaat ggtcaggttc tttcttttga ttcagaagaa tttcaacaaa caagccaagt
    48541 gttgcaacaa ttacgggaat cgatacgtac agaagaagga acgatccatg tacctaaaaa
    48601 tcaaggattg atcattcaaa atcaattgga aaaaagcaat gctactttta gtgaatcgtt
    48661 ccaaacgatg gtacaagatc tcattcatcc agaaagatac caggcacagc ttcccaaagg
    48721 attgaatgca acgatgagag attatcaaaa gcaaggtttt cgatggttga agatgttagg
    48781 tcattatcag tttggcggta ttttagccga tgaaatggga cttgggaaaa cactacagac
    48841 aattgccttt ttactttcgg aaaaagaaga acggaaaagc ttctctgctt tgatcgtagc
    48901 gcctgcgagc ttaatttaca actggcaggc agaggtgaga aaattcgccc cttctttaag
    48961 tatccaagta atcaatggga ataaaaaaga acgtgaagaa ttactggcta aagacacaga
    49021 tatccgagtg acttcttatg ccagcttaag acaggatcta gctaattatc agtcgcaaaa
    49081 aattgattat ttgattcttg atgaggcaca aatggtcaaa aatagcagta cgaaaactgc
    49141 acaagcattg agagaattag ctgtacctca gcggtttgca ctgagtggga caccaatcga
    49201 gaataattta gaagaattgt ggtcattgtt tgcaacgatt atgccaggat ttttcccgac
    49261 taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg caaatgattc gaccgtttat
    49321 tttacggcga gataaacaaa ctgtgttgaa agacttgccg gaaaaaacag agatgaatct
    49381 atattctgca cttacagaag aacaaaaaac tgtttattta gcttacttgc gtcaaatgcg
    49441 tgaagaaatc acatcaatgg attctgaagc attcaagaaa aaccggatcg ggatacttgc
    49501 tggtctgaca agactgcgac aaatttgctg cgatcctcgt ctgtttattg aagattacca
    49561 aggcggttcc ggcaagctcg aacaagtaaa agatctactg gtagctgcca aagagaacaa
    49621 acgaagagtt ttgctgtttt ctcaatttac agggatgctg actatcttgc aagaagagtt
    49681 agccgagcta gggatctcga cattttattt aagaggcagt acgaaaccac aagatcggtt
    49741 aagtatggta gacgcattta atgctggcga aaaagatgtt tttctgattt ccttaaaggc
    49801 tgggggaact ggattgaatc tcacgggagc agatacggtc atcttgtatg acctttggtg
    49861 gaatcctgcc attgaagaac aagctgctgg acgtgcacat cggatcggac aaaaaaatgt
    49921 ggtagaagtg tggcgtatga tctctgaagg aacgattgag gaacgcatgg attcactcca
    49981 gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt aatgaagaac agttgacgaa
    50041 actgactgaa gaagacatcc gcatgatttt aagtgtagga gaaatcgagg agtaagagac
    50101 tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa agaagcgatt gtttatttga
    50161 tttaaaaaat agcaaggagg ctgggattcc attttatccc agcttccttg ctatcatttt
    50221 tttatttcta tttttaaagg ttatacaaag attgtcttca ttgtctctaa cagctgatca
    50281 aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa ttttgcgttc tcgatggttg
    50341 aaccaaagcg cttgccagtt agcacttttt gcacctagaa catcattgtc gtaattatcg
    50401 ccaacgtata aggtctcttc tggaagcata tgaaattctt tctcagctag ctgaaaaatc
    50461 tctttttctg gtttttggaa acctgttgct tgagaaatga gcatattgcg ggaaggaatc
    50521 caattgttca attgaagttg cttcagtttt tttgtctgat ggtcagtcgg cccatttgtg
    50581 ataattccta atgggacatt cttttccttc aagaaatcga gggttttttt gacctcctca
    50641 tgcaagcaga tattatcaag ttcttcctcg taaatttttt gaaataagag gccatcttct
    50701 tccgtaatat gagggtagtc taaatctttt agtgattggc tgatccgatg tgctcgcata
    50761 tattctagtg tccaatctcc tgccattact tttgggaagt tttcatcact atgatggcgg
    50821 aaccggatat atagtggatg catatctgca tctgaaacaa gcggaacgac tcttttgacc
    50881 gcgtttcgaa atggctgctg ctgatcatac atcgtatcat caacatcaaa cacaactgat
    50941 ttaatcaaaa ttttacacgc ctttcggttt tcattgattt tttcacaaat ccttgactat
    51001 tgtaacagaa aaaattgtct aaaaaagctg ataaataaag gtttttctta ctatatttct
    51061 atttccttga cagaataagg tttttatatt tcagtttttt tcactaaaaa tcgtgaaaca
    51121 cgtcaataag tttgatgaac aaattaatca ttatggtaga agtcgattga tttattttaa
    51181 tgcaatctct gtggaatttt cgaaagattt atttttttaa tacagtctca atataactat
    51241 tcaaacataa aataacgtta tttgatttat ttgtttatag taaattttat ttagtaatca
    51301 ttgctttaaa atactacgct tatataacag agaaaataag cgttataata aagaacagta
    51361 ttatttaaaa tgacagaaaa cattactatt aaagaaataa aaaacataaa aaatcttaag
    51421 aaatcggttt catttagctt ttgagataaa ataatagtac agttttatac gctgtttcat
    51481 atgtttagaa aatttgaatt agatttgaaa actctaattt ttaaatgata aaaaaagcga
    51541 gaatgctggt tttactgtat attaacctta gataactttc atttttttaa aaaaattatg
    51601 aaaaaaaggt agctatttaa aagttgatta tgtataatag aaacagatgg gagattatta
    51661 tgatcttctg ttacaaaaaa gtgattctta tcacgaaaag gagtggtgca cgttgttaac
    51721 attatatact tccccaagtt gtacttcctg cagaaaggct cgtgcgtggt tgcaagaaca
    51781 tcagattcca tttgttgaac gtaatatttt ttcagaacca ttgaacagtt ctgaattaaa
    51841 agcgatttta caaatgacag aagacggaac agaagaaatc atctcgacac gttccaaagt
    51901 attccaaaaa ctgaacatgg atttagatga tctgccgtta caagaattat tggaacttgt
    51961 gcaaaacaat cctggattgc ttcgccgtcc aatcatgatt gatgataaac gtctgcaagt
    52021 tggttttaat gaagatgaaa ttcgtcggtt cctaccgcgc gatgtacgac aattagaatt
    52081 acgtcaagca caattgatgg ctggattata gaaggataat acgaaacttg gaaaagacta
    52141 ttacaaagat agtttttcca agtttttttg tttttacaaa aagtattctt gattagatgt
    52201 ataaaaaggt agtagaagat gatgattgca cgaaaaatag agagggaaaa ttagctgaaa
    52261 agtcggctat atagtattcg tctgccaaaa atgttatagt taaataagat agcttgaaag
    52321 gttgaaaaat ccgctggttt tattaaattc gcttaacagc gagatttttt ttctttacat
    52381 ttaagctgat ttcgctacaa tgaatctata ttaaacattg tggcaaagtg aggtgtagca
    52441 aaatggaaat ggaacatatt aatgaaaata cgatccgtgt actaattggt catgaagatt
    52501 tagaggaacg gggcgtttca tttttggatt tacttggcaa tcacaaagaa atcgaaaatt
    52561 tcttttatag tattttggaa gaagttgata tcgaagagga gttccgagga agcgaagctg
    52621 tcactttcca agttttgcca aaaggagacg gattagaact gtttatcagt aaaaatcttc
    52681 cggatgaaga aatttctcaa tcagaagatt ctactgttga ttcagaagat gtgagtgact
    52741 ttcttcgtca gcagattatt ggcagcgatc cggaggaaga attatctgga agcgccctag
    52801 aagaacagga aagacaagca acttttgctt ttgaagattt tgaacaagtg atccaacttg
    52861 caacagatgt cgaactagag tctgcttgga ctgatttgta tttattcgaa gagcgctatt
    52921 acctagtcgt tcacttctgg atggaaaatc tcaaccaagc ggatgtagag aatcagattg
    52981 ctcgaatttt ggagtttgct gaaaagagcg atcgtacacc agaaacattg aatgaatatg
    53041 gacaatgtct gatggcgcgt aatgctatcg agcgaacgaa attttatttt gtataaatca
    53101 gcattatagt aagagactgt gacaaaaatt gtgtcgcagt ctttttttcg tataaatgtg
    53161 ttcaaaagct tgcatctgcg gtaacaagcc caaccaaacg aaaaatattg ggaactattt
    53221 ttgtttagct gttcttatta cttgatgcag aatagctttt tcacgccctc ttttttcgtg
    53281 taaataaatt cttagacgta aataagttca gagggagaaa aatgctgctt gcaatgaatg
    53341 aatataataa gtgctgtttt gctaaacatg cagaaaaagg aaagaaatat tattgtcgtg
    53401 gctgtcgaaa aagagtgatt ttgaaaaagg gaaagaaaaa atgtgctcat tttgcccatc
    53461 aaaaatcaga taactgttcg gtctttagtg aaagagaatc aaaagagcac ttacagttaa
    53521 aggaatgctt catggattgg ctgggacagt cagtagaacc agtatttttg gaagcctatt
    53581 tgccgaggtt gcggcaaagg ccggatattt tgttggcgaa tctagcaata gaaatccaat
    53641 gttctagact gtctcaccag agatttattg aaagaaccca aaattatttg aataacagtt
    53701 accaagtatg gtggatactt ggtaattctt ttcttggaca atcgcaattc tcattgatag
    53761 aaaaaagttg ctgctactat aatagaaaaa gaggagtgca ttgctggaaa gctgatctca
    53821 agaaacaaaa gctttatttg tatcatcata ttacagagac tgtatcaggg catatttcct
    53881 tttgcagtag ctgctggact ttttctagta gggaccttaa agaaatattc actagtaacg
    53941 aaattaaaat gaaccaaatg aaaaaaatag aaaaagtgag tgaagatggg aagaaatggc
    54001 tgacacgcca acttattcac aagcaaaaga atacagtatc catacaagaa caatgctact
    54061 tgcgtcataa acatcttttg catctatcat cgtggatcta tcaaaaaagt cggtttttct
    54121 tttatttaca agaacaggtt tttctttatc gaatgttgta cgaagaagcc ttggaaaacc
    54181 agaaagttcc tgatttcaac tcatggcttt gccaaataaa agaacataaa agggaatggc
    54241 tttttcctat gattgatgaa gagatggtct atcagcagtt ttttaacgaa tgcatacact
    54301 tgtcttcttt gaaaaaatag taagtataaa aataggctag gaatcggttt tctatgtaaa
    54361 acaagattta gttatgctaa aattgatgga agcagacaca tgaatgagta gaaaagttag
    54421 atgctgtgac atgtggatgt tagactatga tctattttga tagatagagc taagggaaga
    54481 aaaggagtgg atttcatgga agtaaagcag ttgccaaaac gagaagaatt gcctgaaaat
    54541 ttaacttggg acttgaccaa gatcttttca agcgaccaag agtttgatga gaaatatttg
    54601 gaattatcag aagagttaaa acaatctgaa aaacacaaag gaacacttga tcaaggcgct
    54661 tctcaatttt taaatgcgat tgaattcgta ttgagggttt atcgccaaac tgaagtcatt
    54721 tatgtatatg cgcaccttaa aaacgatcaa gacactggaa atacagatta ccaagcgctt
    54781 tatgcaagag caagcagtct gttttcgaaa gttagtgaag ccgtttcctg gtttgaacca
    54841 gaaatattgc aattgtcaga tgaccagatt tggcaatatt tcaaagaaga accaaaattg
    54901 gaagtctatc gccattatat ccagcaaata gtagataatc gagcccatgt cttatctgct
    54961 gagcaggaat ctcttcttgc tggagcaggt gaaatctttg atgcttcaag tgatacattt
    55021 gctgttttga ataatgcaga tctagttttt ccaacgattg aaggagaaaa tggtgaaata
    55081 gtccaattat ctcatggcgt gtatggtcag ttgctagaaa gcacggatcg aagggtgcgc
    55141 gaagcagcat ttaaggggtt gtacagtgtt tacgaacaat ttagaaatac atttgcttct
    55201 actttaggca cacatataaa aggacataat tttaaagcga aagtccgtaa ttacagctct
    55261 gccagagaag cgtctttgag caataatcat attcctgaaa gtgtatacga tactttggta
    55321 gacgtggtaa acaagcattt gcctttgtta catcgataca tggaattacg gaaacgttta
    55381 ttagaagtgg aaaaactgca catgtatgat ctttatacac cggtcttagg ggaagctcca
    55441 attaccttta cgtacgaaga agcaaaagaa aaagctttag aagcactgaa accaatgggt
    55501 gaagaataca tggccatcgt agaaaaagca ttctctgaac gttggatcga tgttgtcgaa
    55561 aataaaggga aacgaagcgg tgcttattct tcgggaagct atgacacaaa tccatatatt
    55621 ttattgaatt ggcatgatac gctggatcag ctatttacgc ttgtccacga aatgggacat
    55681 agtgttcata gttatttcac tcgttcgaac cagccttatg tgtacggcga ctactccatc
    55741 tttttagcag aaattgcttc gacaacgaat gaaaatatcc taacggagta tttattggaa
    55801 acagaaaaag accctcgtgt acgggcttat gtactcaacc attacttgga tgggtttaaa
    55861 ggaacagttt tccgccagac acaattcgct gaattcgagc atttcatgca tacggaagat
    55921 gaaaaaggtg tgccattgac tagtgaatac ctaagtgata gttatggtaa attgaatgca
    55981 aaatattatg gtccagcagt cgaagaagac ccagaaatta aattcgaatg gtcacggata
    56041 ccgcattttt attataatta ctatgttttc caatactcga ctggcttttc tgctgcttca
    56101 gcactggcga agaaaatact aaaccaggaa ccagaggcac tagagaacta cttggcttac
    56161 ctaaaagcag gtaacagcga ttatcctgtg gaagtaatga aaaaagcagg agttgatatg
    56221 acacaggctg catatattga agatgcaatg tcgatgtttg aacaacgtct aaatgaatta
    56281 gaagaattga tcgatcgttt ataaggcttt ctatttttga ttttctcgaa tagaaacgaa
    56341 aaaaatacgt cggctctctt gtaagggagt cgacgtattt ttt
//
LOCUS       DADXTH010000008        53772 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.008, whole genome shotgun sequence.
ACCESSION   DADXTH010000008 DADXTH010000000
VERSION     DADXTH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.008"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IWI97_000467"
     CDS             11..733
                     /locus_tag="IWI97_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBC2461999.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IWI97_000468"
     CDS             791..2830
                     /locus_tag="IWI97_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBC2462000.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLLAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="IWI97_000469"
     CDS             complement(2939..4090)
                     /locus_tag="IWI97_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBC2462001.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="IWI97_000470"
     CDS             complement(4244..4774)
                     /locus_tag="IWI97_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBC2462002.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="IWI97_000471"
     CDS             complement(4873..5223)
                     /locus_tag="IWI97_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBC2462003.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWI97_000472"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWI97_000472"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBC2462004.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWI97_000473"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWI97_000473"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBC2462005.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="IWI97_000474"
     CDS             complement(7626..8288)
                     /locus_tag="IWI97_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2462006.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="IWI97_000475"
     CDS             8472..10118
                     /locus_tag="IWI97_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBC2462007.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..11191
                     /gene="uxuA"
                     /locus_tag="IWI97_000476"
     CDS             10109..11191
                     /gene="uxuA"
                     /locus_tag="IWI97_000476"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBC2462008.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11299..12852)
                     /locus_tag="IWI97_000477"
     CDS             complement(11299..12852)
                     /locus_tag="IWI97_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2462009.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13000..14288
                     /locus_tag="IWI97_000478"
                     /pseudo
     CDS             13000..14288
                     /locus_tag="IWI97_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14414..15493)
                     /locus_tag="IWI97_000479"
     CDS             complement(14414..15493)
                     /locus_tag="IWI97_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2462010.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15596..16036)
                     /locus_tag="IWI97_000480"
     CDS             complement(15596..16036)
                     /locus_tag="IWI97_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2462011.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16093..16503)
                     /locus_tag="IWI97_000481"
     CDS             complement(16093..16503)
                     /locus_tag="IWI97_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBC2462012.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16683..17117)
                     /locus_tag="IWI97_000482"
     CDS             complement(16683..17117)
                     /locus_tag="IWI97_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2462013.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17217..18065)
                     /locus_tag="IWI97_000483"
     CDS             complement(17217..18065)
                     /locus_tag="IWI97_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2462014.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18454..20220
                     /locus_tag="IWI97_000484"
     CDS             18454..20220
                     /locus_tag="IWI97_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBC2462015.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20322..21581
                     /locus_tag="IWI97_000485"
     CDS             20322..21581
                     /locus_tag="IWI97_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBC2462016.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21728..23029
                     /locus_tag="IWI97_000486"
     CDS             21728..23029
                     /locus_tag="IWI97_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2462017.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23032..23883
                     /locus_tag="IWI97_000487"
     CDS             23032..23883
                     /locus_tag="IWI97_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2462018.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23896..24711
                     /locus_tag="IWI97_000488"
     CDS             23896..24711
                     /locus_tag="IWI97_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBC2462019.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24862..26028
                     /locus_tag="IWI97_000489"
     CDS             24862..26028
                     /locus_tag="IWI97_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2462020.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26258..26905
                     /locus_tag="IWI97_000490"
     CDS             26258..26905
                     /locus_tag="IWI97_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBC2462021.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26989..27933
                     /locus_tag="IWI97_000491"
     CDS             26989..27933
                     /locus_tag="IWI97_000491"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBC2462022.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28068..29519
                     /locus_tag="IWI97_000492"
     CDS             28068..29519
                     /locus_tag="IWI97_000492"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBC2462023.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29540..30376
                     /locus_tag="IWI97_000493"
     CDS             29540..30376
                     /locus_tag="IWI97_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBC2462024.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30376..31035
                     /locus_tag="IWI97_000494"
     CDS             30376..31035
                     /locus_tag="IWI97_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBC2462025.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31161..31850
                     /locus_tag="IWI97_000495"
     CDS             31161..31850
                     /locus_tag="IWI97_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462026.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31856..32812
                     /locus_tag="IWI97_000496"
     CDS             31856..32812
                     /locus_tag="IWI97_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBC2462027.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32856..33368
                     /locus_tag="IWI97_000497"
     CDS             32856..33368
                     /locus_tag="IWI97_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2462028.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33624..34172)
                     /locus_tag="IWI97_000498"
     CDS             complement(33624..34172)
                     /locus_tag="IWI97_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2462029.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34261..35442)
                     /locus_tag="IWI97_000499"
     CDS             complement(34261..35442)
                     /locus_tag="IWI97_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBC2462030.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35678..37186)
                     /locus_tag="IWI97_000500"
     CDS             complement(35678..37186)
                     /locus_tag="IWI97_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBC2462031.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37345..37536
                     /locus_tag="IWI97_000501"
     CDS             37345..37536
                     /locus_tag="IWI97_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462032.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37739..38656
                     /locus_tag="IWI97_000502"
     CDS             37739..38656
                     /locus_tag="IWI97_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBC2462033.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38649..39722
                     /locus_tag="IWI97_000503"
     CDS             38649..39722
                     /locus_tag="IWI97_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462034.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39870..40388
                     /locus_tag="IWI97_000504"
     CDS             39870..40388
                     /locus_tag="IWI97_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462035.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40369..40761
                     /locus_tag="IWI97_000505"
     CDS             40369..40761
                     /locus_tag="IWI97_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBC2462036.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40837..41772
                     /locus_tag="IWI97_000506"
     CDS             40837..41772
                     /locus_tag="IWI97_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBC2462037.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41905..42684
                     /locus_tag="IWI97_000507"
     CDS             41905..42684
                     /locus_tag="IWI97_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBC2462038.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42677..42994
                     /locus_tag="IWI97_000508"
     CDS             42677..42994
                     /locus_tag="IWI97_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBC2462039.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43238..44848
                     /locus_tag="IWI97_000509"
     CDS             43238..44848
                     /locus_tag="IWI97_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBC2462040.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45005..45853
                     /locus_tag="IWI97_000510"
     CDS             45005..45853
                     /locus_tag="IWI97_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBC2462041.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46084..46563
                     /locus_tag="IWI97_000511"
     CDS             46084..46563
                     /locus_tag="IWI97_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462042.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46596..48824
                     /locus_tag="IWI97_000512"
     CDS             46596..48824
                     /locus_tag="IWI97_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2462043.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            48953..50596
                     /locus_tag="IWI97_000513"
     CDS             48953..50596
                     /locus_tag="IWI97_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBC2462044.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50669..51214)
                     /locus_tag="IWI97_000514"
     CDS             complement(50669..51214)
                     /locus_tag="IWI97_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBC2462045.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51237..51722)
                     /locus_tag="IWI97_000515"
     CDS             complement(51237..51722)
                     /locus_tag="IWI97_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2462046.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(51712..52524)
                     /locus_tag="IWI97_000516"
     CDS             complement(51712..52524)
                     /locus_tag="IWI97_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBC2462047.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(52663..>53772)
                     /locus_tag="IWI97_000517"
     CDS             complement(52663..>53772)
                     /locus_tag="IWI97_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBC2462048.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        17905 a         9784 c        10381 g        15702 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgcttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatataccag taggagaggt atggcccagg gagaggatca aagaactaaa
    10261 agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca atgtgcatga
    10321 tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata agcagacaat
    10381 ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc ctgtatttga
    10441 ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt tagcttttat
    10501 atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac attccagcaa
    10561 tgaatttgaa ttgccgggat gggaacctga gcgcttagct catatcaaat ccttgcttga
    10621 agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt ttctccaatc
    10681 aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag atgatccgcc
    10741 atactcgatt tttggattac ctcgaatcgt aaagaatcga gaagatttag attggttgtg
    10801 ccgcatagta gacagccctt ataacggaat cactctttgt acagggagta ttgcagaaga
    10861 tccagaaaat gatgtatatg ggattttagc agaattttgt caaagagatc gaatcccatt
    10921 tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg caccgcataa
    10981 atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg acaacggatt
    11041 taacgaatac atccgtcctg atcatggaag aatgatctgg aatgagacag gaagaccagg
    11101 atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat gggaagcaat
    11161 cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa
    11221 taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa gcattcggat
    11281 ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt gatccatgag
    11341 tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt ttttccagtc
    11401 ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag gcaattgttt
    11461 caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt gtattttgat
    11521 cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga tataaaaatc
    11581 acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat catacaaatg
    11641 tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg gtaacaagtg
    11701 ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga cttctttcaa
    11761 cacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt gattgaagtt
    11821 aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt
    11881 ttcaggtata aacaaatcaa atacagtaca aaaggactcc accatttctc ttgaataaag
    11941 aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg tttcttccga
    12001 ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt gttcgcgaca
    12061 aattggcaga ttaggtcgca aaatatctac agagggatca ataaaaaagt caatatattc
    12121 atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat tctctttaca
    12181 tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt caagtacttc
    12241 ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat cataaaattc
    12301 tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt gaatttgtac
    12361 ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca aaccgtacaa
    12421 tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac tatagtttgt
    12481 ttgctgactt aaactgataa tcgaatttgt ctgacagctg aacaaagaag tcaagatctg
    12541 ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt aagccggatc
    12601 aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca gttctgttag
    12661 attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat ttgattcaga
    12721 catatggaaa tgatctgcta aatattgtgt agtaataaag aaacgatgtt cagataggaa
    12781 aagaaaaata tctaattttg tattgctttt tttcttcaag agaaacgttt taatcgcttc
    12841 ttgagtttgc atgaaagagg ctccttttta cttaaattgt taaaacttga aaatattact
    12901 gtttttttca taaatttaga caaaaaaaca gtaaacatat aggtagaatc tctactatct
    12961 tatttcttgc atacaaaaaa atcaaggagt gagagagtta tgagatattc aaccatctta
    13021 atatcaacat ttgcagcctt aagcttatcg cttactataa atcagacagt ccacacagag
    13081 gagatagaaa cagccaaatg gacagaaacc aagtgggcca acgtcaatcg aattaccttt
    13141 caggatgaat tttcatcctt caattcagga gtcgcaaaat taagaaaagg acaaacagcg
    13201 accatcaaag caaaaattga ctattcagga gacttagcac ctatactaca atcagctgtt
    13261 acatttgaga atgttgatcc cgctttgtcg attggctatg acgagaacac tttgactatc
    13321 caacacaagc aagcgatatt taccttcacc gttacattaa atcaggactt gttaaaacca
    13381 gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg atcatcatca tttaacgccg
    13441 tatagtcagc ggcagacaat tgagcaagat tcagctatcg actcaggagg agatcttgtt
    13501 gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc tacagaaaat
    13561 ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac agaaactcaa
    13621 ccagagccag acaatgaatc aaaacctgaa ataacccctg gagaaaaacc agatgtagac
    13681 ccagaagaaa aaccagatgt gactccagaa ccagatacgg attctggaaa ccaaacagta
    13741 ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagcc
    13801 ccagaagaaa aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta
    13861 ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat
    13921 ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata
    13981 ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca
    14041 aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat
    14101 agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa
    14161 cgaaaaataa acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg
    14221 gaatcattct attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat
    14281 ctctctaaca tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga
    14341 aaatgacccc aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt
    14401 cttcatattt attttaaggg atatctgttt cttttggaag atggttcggg atctttttca
    14461 ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc
    14521 caaagaaccg ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt
    14581 tgctggacat caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg
    14641 taatcaggat ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag
    14701 gaatacaaga aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg
    14761 ctaaactcag cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat
    14821 ttaaaagagc gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat
    14881 cggcaaaata gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg
    14941 agaatacaat tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag
    15001 caaacgccac tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat
    15061 tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag
    15121 gatctgcatc ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg
    15181 tttgatgaat aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca
    15241 gtgtataaat catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt
    15301 gaacaagatg aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac
    15361 ttctaactaa gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt
    15421 cattcgccaa aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa
    15481 acacgttatt cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct
    15541 aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc
    15601 cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat
    15661 ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt
    15721 gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg
    15781 tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg
    15841 taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc
    15901 ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag
    15961 tgtagtgtct aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta
    16021 aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt
    16081 acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc
    16141 ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga
    16201 aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt cactttctga
    16261 ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat
    16321 caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt
    16381 ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg
    16441 attatgcact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt
    16501 cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg
    16561 gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc
    16621 tttctcgaat agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa
    16681 cattacatat atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc
    16741 tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag
    16801 tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt
    16861 gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta
    16921 ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag
    16981 gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca
    17041 tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt
    17101 tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt
    17161 ttattatacc agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat
    17221 tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca
    17281 aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc
    17341 acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg
    17401 gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca
    17461 ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt
    17521 gaagtgattg caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga
    17581 ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa
    17641 tattttttaa aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat
    17701 aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct
    17761 gaagtaatca ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa
    17821 agtactttaa aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata
    17881 ttcaacacta aaataactat agctcggtca aagcctaaag cagcttccag taatattcct
    17941 agaccgctga cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact
    18001 cctaatatga agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct
    18061 ttcatttatt cacttcctta attcattaat aatactctta tcttattata acttactttt
    18121 caccttaagt taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat
    18181 tcttctagaa tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg
    18241 agaaattgat cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac
    18301 ttttaaaagt tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa
    18361 aaactgttac cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg
    18421 atgacgtcat attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga
    18481 ccggaaagtg aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact
    18541 aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact
    18601 gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat
    18661 tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt
    18721 ggggaagata acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat
    18781 gtgattactg aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc
    18841 actattccag aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca
    18901 aacggggaca aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac
    18961 cgagaagact ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg
    19021 gaccttggca tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa
    19081 tacgatacga ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa
    19141 aacttgattg atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat
    19201 catatgggtg tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc
    19261 tataaagact ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta
    19321 ccagaaactg ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa
    19381 ctaaatacag caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata
    19441 aaagaattcg atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt
    19501 tggaaaaaat tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag
    19561 atttggcatt cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat
    19621 gcatttacag attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg
    19681 gtcagtggaa tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc
    19741 aacttattag attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa
    19801 ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac
    19861 gggacagaaa tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg
    19921 gagaaggagg aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg
    19981 aaacagttgt ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt
    20041 gaagataagc tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat
    20101 cagtcaaaag aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt
    20161 cagttgttag aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga
    20221 aaactgttac cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat
    20281 aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga
    20341 aacagttggg tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca
    20401 atggtaactc ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg
    20461 attctggata taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg
    20521 tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg
    20581 ggcctgctgg aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag
    20641 gacagcaagg acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag
    20701 acaaagccca agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc
    20761 ttgttttgta ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg
    20821 agaatctatc caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat
    20881 ttttagctaa atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct
    20941 atgtatttgg tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg
    21001 tagaaggaat ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag
    21061 acataaaaag tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa
    21121 tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag
    21181 ccaaaattcc tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat
    21241 gggtagtcag caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa
    21301 cgaatcagga aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac
    21361 aagctaggga aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat
    21421 atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag
    21481 aaaacttgat gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag
    21541 cagtaaaaac aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca
    21601 tttttataaa aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt
    21661 atcttgctct tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg
    21721 cattcagatg cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc
    21781 acttattcca gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt
    21841 cgccatattc gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg
    21901 attggttact ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg
    21961 cactttgcaa ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga
    22021 cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga
    22081 agtcatacat aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct
    22141 tctcatgatt tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa
    22201 ttatgacttc aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt
    22261 ttttagtatc ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac
    22321 cgtgatttgg actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt
    22381 tgttgctcac cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc
    22441 atgggcggtc ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt
    22501 aggtgccatc aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag
    22561 tgcaatctct tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg
    22621 ctggttagga tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga
    22681 agacttatat gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat
    22741 cacgcttcct gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa
    22801 tttcaataat ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg
    22861 aggagctggt tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc
    22921 tccgcagtat tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt
    22981 gtcattgctg gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa
    23041 tttcattcga gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt
    23101 ttaatactgt cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag
    23161 tcaggaaata ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt
    23221 actcggctat tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct
    23281 gtcctaacaa tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc
    23341 tatcgtttta tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg
    23401 actatggcag cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag
    23461 tactggttct tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg
    23521 aaaggatact ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca
    23581 gggcatttca ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt
    23641 caagcattat gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt
    23701 cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca
    23761 aaccaaagag tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta
    23821 ttattcttct ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga
    23881 taggagcaga gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca
    23941 actcaagtgt ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta
    24001 tttttagttt ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg
    24061 attccattag aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa
    24121 ttttctgaac ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg
    24181 acccctgaaa ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact
    24241 tttattgatt ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc
    24301 cgctatacga tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat
    24361 ttccaacgat ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct
    24421 tatagtttaa gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct
    24481 tcctttttct tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag
    24541 agtgcaaatt tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg
    24601 tttatccact ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg
    24661 cttttatggg tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa
    24721 gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt
    24781 ctgaaaaaat taaacttctt agttgattct tttctttttt agtattaacg taatagggta
    24841 gtttttttaa ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt
    24901 tctttctcgc tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat
    24961 acggacactc ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt
    25021 acaagtaacg cttctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag
    25081 ggaagtatcc aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga
    25141 aaagattatc taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa
    25201 gatatccatg cgcattatga cttaggtaac gacttttata aactttggct agatccaact
    25261 ttgacttatt cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc
    25321 aataaagtcc atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat
    25381 atcggttgtg gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca
    25441 actgggatca cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa
    25501 catttagaag ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg
    25561 tttgatcata tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt
    25621 tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc
    25681 agccgacaac aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt
    25741 tatgtccctg gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt
    25801 gatttggaaa gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt
    25861 catcaagtcc aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat
    25921 ctatatttac aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat
    25981 ttgcttgttc aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag
    26041 tactgtttaa agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt
    26101 tttgtctaac ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa
    26161 taaaagtttg gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt
    26221 aatagaatac tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac
    26281 tagtttattt gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc
    26341 tgggaatacg tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa
    26401 agcaagcaca tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga
    26461 tatcaaagtg tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc
    26521 aattactagt ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt
    26581 cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga
    26641 gaatccagaa actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct
    26701 ggatgttgat aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg
    26761 aagcggtacg gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt
    26821 caaagtaaaa gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt
    26881 actgtcaact gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt
    26941 tagtcatgat acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa
    27001 acgacctttg ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc
    27061 atgactaccc aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag
    27121 agctgtacga tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta
    27181 gggagtatca attctatggg acttccaaac ttaggttttt cttattatct agagtatgct
    27241 cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg
    27301 atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg
    27361 atcactgaat taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat
    27421 tttgaggcca cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga
    27481 atcaaattgc ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat
    27541 cagtttccat tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat
    27601 acagaacaag aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag
    27661 tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa
    27721 atccaaatca ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta
    27781 tgtggcgcaa gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt
    27841 tctagaatca taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac
    27901 gaatttaaag gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct
    27961 tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat
    28021 gttagacgaa cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa
    28081 cagaattagc aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata
    28141 tacagaaaaa aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat
    28201 tcgagagtaa ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta
    28261 tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat
    28321 ctcgtttatt tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag
    28381 cgataggtct agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta
    28441 cagacccagt catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg
    28501 gatcaagtgg aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta
    28561 gtgatggcgg tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac
    28621 cttcgcgagg gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta
    28681 ttgatttcgg cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa
    28741 gtatccattc cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa
    28801 aaaaacggtt gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag
    28861 aggcagtact tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga
    28921 cagagattcc ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg
    28981 gcgcagaaac agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa
    29041 aagaagaact tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg
    29101 ctacttacat cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac
    29161 atcaagaata cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg
    29221 aagatttgca aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag
    29281 tagaaggttt agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata
    29341 cacagttggc taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag
    29401 aaaacatgcc tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc
    29461 aaatcggcta tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc
    29521 agaaacaagg agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt
    29581 ttttatttta agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa
    29641 tgtagaaaaa aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta
    29701 ttataatgat caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa
    29761 acggttaaat gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt
    29821 ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa
    29881 aaaattctcc ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa
    29941 agatgattca ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc
    30001 caatactagc tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa
    30061 tgccacgaat gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa
    30121 tgattattat ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact
    30181 ggagaacgtt tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc
    30241 tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa
    30301 aatatctgaa aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga
    30361 gtcggtgagt gagtgatgta ctttcctcta attgattttt ccctcatgtg gacttcactt
    30421 cctttcgtac tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta
    30481 ggaaatctgg tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat
    30541 gtatttattc gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt
    30601 ctgttatatt ttgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt
    30661 acgattacca gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct
    30721 tcaggtcagt gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat
    30781 atcgttttgc cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat
    30841 ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca
    30901 aagatcatag gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt
    30961 tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg
    31021 catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt
    31081 gtttcttgta tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa
    31141 tatgttcgga ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat
    31201 accaatattg catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc
    31261 aaggaattgt agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat
    31321 gctatgagct gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg
    31381 gacattataa ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc
    31441 ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct
    31501 ctggtacatt tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag
    31561 ctttgacaaa ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg
    31621 agcgaggcgt aaaatatttt ccaacagatc aattgtacaa agaattgaaa agacgttcag
    31681 aaaatcaaga tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta
    31741 gtcaggcaat caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt
    31801 tgaggcaaca acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta
    31861 ctatccttat ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg
    31921 aggattgctc tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct
    31981 taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc
    32041 ccaagtagga caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa
    32101 tagctctgtc gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc
    32161 acctccagcg tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa
    32221 acagttggca ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg
    32281 gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag
    32341 ttatgcagat aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg
    32401 atatattgga ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc
    32461 tagccgagcg cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa
    32521 acattttgtc tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc
    32581 aggagataaa atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg
    32641 aatgattgaa ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa
    32701 aaagtggtca cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga
    32761 ttggcttaaa tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa
    32821 tgtaaatgaa aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag
    32881 aagaagtaga agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag
    32941 gctctcctca atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg
    33001 aaagaaaaga aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag
    33061 tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc
    33121 cttatttatc gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa
    33181 aaccgttctt agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta
    33241 tcgatactta tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt
    33301 atcaagggat gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca
    33361 tcaattgact ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca
    33421 atcatatgat agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat
    33481 ttccgaaaat gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt
    33541 tctgaaactc ccttcatcga gaggtcgtga catagctttt gtcacgacct ctcggcttat
    33601 cctacgagta ttattcgata catttatatc aataatcctt tttttatttt atctgcagct
    33661 tcttggaacg ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc
    33721 aactgtgcta aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct
    33781 ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg
    33841 actaccgtcc cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa
    33901 caatttccta ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga
    33961 gcatattggt cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca
    34021 gaaacaaaat aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa
    34081 gtaatgatct tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca
    34141 gaaggaccga taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa
    34201 gtgaaaaagc tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtata
    34261 ttataccagc aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa
    34321 aattttcaat ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc
    34381 accagaaatg atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat
    34441 tgctgttgta cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa
    34501 ttcatttgta accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg
    34561 gattccgctg atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat
    34621 gccgaatatc ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag
    34681 catagcagca accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg
    34741 gaaaaatgtc tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac
    34801 atagggattt acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt
    34861 gatcacatat ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt
    34921 agacatagcc gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt
    34981 ttttatggaa ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga
    35041 ttccaatttg cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa
    35101 tggaagaatc ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat
    35161 caatacggaa agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc
    35221 aacaccttgc ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac
    35281 cgctgtacca aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat
    35341 cacaattagt ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc
    35401 taatgtgaca aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca
    35461 aattccgttt tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat
    35521 tactttttta gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa
    35581 ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct
    35641 aatagtttcg ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg
    35701 aagggtagac aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat
    35761 ttttattgac catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat
    35821 aagcaacttg agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat
    35881 tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt
    35941 caagaactaa tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg
    36001 catttaagat aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga
    36061 cttcatctgg taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt
    36121 gaatcttatc tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac
    36181 cgacttcttc cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt
    36241 tgaagtcgca ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga
    36301 caacggtatc atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt
    36361 gataaacaga ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct
    36421 caacatcccg attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga
    36481 tcatattgac atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt
    36541 tcaacggaac attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt
    36601 tttggctttc atgatacagt tggttcaagg aagtagcgag ctttccactt cctgggccag
    36661 gagctgtcac tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat
    36721 cttctgagac gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga
    36781 ccttgatgtt tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat
    36841 agcgagtgat aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc
    36901 gcaagatatc catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat
    36961 ctccagcata gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga
    37021 ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactagct
    37081 tcccaccaaa ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg
    37141 actgctcttc aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt
    37201 aagtactaaa gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct
    37261 caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat
    37321 agtttttgat aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa
    37381 cactatatcc gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa
    37441 caattgacgt ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc
    37501 attcatttca caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat
    37561 tcgattctgt cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt
    37621 tggtgcagcc tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa
    37681 atggtactat tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat
    37741 gaaagagcca gatacaaatg caaattatga gccagacgaa ctagaggaga cgaatgcgga
    37801 tcaggataca ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa
    37861 tctttcccaa ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat
    37921 ccctgagatc taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa
    37981 tgaaaatcac gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga
    38041 atttcctttt atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg
    38101 cacttattac catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt
    38161 tttgcctaag atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga
    38221 caaaaaaatc aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt
    38281 agatgaacaa ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt
    38341 cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact
    38401 atctaatgaa aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca
    38461 aaaagaagca ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa
    38521 agaacaaagg caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca
    38581 tgaattcttg atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg
    38641 ttccgagtat gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag
    38701 atcatgaaga aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag
    38761 aagaaatccc tatgctagag gaaacaaaaa tacacgaaaa agaaaaagta actccgacga
    38821 atacgaatac tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag
    38881 aaggaaaaga gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat
    38941 tagcggattt acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat
    39001 catatgatcg ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga
    39061 tcgaatactt cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa
    39121 attcagctta tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac
    39181 aaaacgaatt agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat
    39241 cgatgagtct cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg
    39301 tgctttcttt gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga
    39361 tattcaatca caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta
    39421 atcagacatt actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat
    39481 tgacgaatga agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata
    39541 tggaaaagat caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga
    39601 taacaacaaa cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat
    39661 ctgtcattga tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct
    39721 gattggataa gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg
    39781 cagttatcca gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac
    39841 ttgctgatga aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct
    39901 tattgtctgc tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc
    39961 caatgactgc tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg
    40021 tcatgagtct ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg
    40081 gccaatggat cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa
    40141 cagtgcaaag gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg
    40201 gaataggtat gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt
    40261 accttcaagg ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg
    40321 tttttctgcc agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata
    40381 atttatagtt tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc
    40441 tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc
    40501 acgttacaag aaaatggtca aaacttttcg gaaaaagaag tgttcttcaa aaaaggcgat
    40561 gatttactga gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact
    40621 tcgcttgaag ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac
    40681 gggaaaagtg ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt
    40741 ttcaatttgt ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa
    40801 ttcagtgagt tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga
    40861 atgttttccg taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc
    40921 ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat
    40981 tgcagcaatc agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag
    41041 ttgcatcaat ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg
    41101 cttttcttcg gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca
    41161 tcccatgaag tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc
    41221 gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt
    41281 tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa
    41341 gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca
    41401 accgggattg ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga
    41461 gccgccattt tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca
    41521 agcgatttat caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat
    41581 ataaaaacag ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt
    41641 ggtaacaaag agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag
    41701 tctcttcctg acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat
    41761 tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt
    41821 ttttaaataa aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt
    41881 gttgtaatta aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct
    41941 ttgcatgcag tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta
    42001 ttgttgcaaa aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt
    42061 tccggtggcg cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg
    42121 acattattga tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc
    42181 ggttttatga aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat
    42241 gaaaactatg cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag
    42301 gctttatatg tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat
    42361 tttttcggct cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg
    42421 ttcattttca tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc
    42481 tcaggcgtat taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca
    42541 gcaacagtca ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa
    42601 gctaagaaac taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa
    42661 caggaggcca atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat
    42721 cgtggcatat atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc
    42781 ctggttcagt gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa
    42841 agatgttttc attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat
    42901 gattctagta gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg
    42961 acttgcgtct gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag
    43021 attgcgacat aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg
    43081 gatgcaagtt tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt
    43141 tttattttag gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag
    43201 atgtttattt tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg
    43261 aacattttgg ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat
    43321 tccctggcta agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa
    43381 tgttggctat cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc
    43441 gattgcaaaa aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat
    43501 ttttagaagt acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta
    43561 tttatttgct cctttactag ccggaatcag tccgattgct aacgttaata atgggatact
    43621 agctatcaga agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg
    43681 atatttccaa ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc
    43741 tgtgcgagtg atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac
    43801 catattagaa gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat
    43861 tatccatatg tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat
    43921 ttccagccgt tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac
    43981 actaccgttt atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac
    44041 gatgaaacca ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt
    44101 attcagccgt gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg
    44161 aacagtagct ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca
    44221 aatcgaaaaa acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt
    44281 gactggaatg tctttattag ctggaccact atacacattg ttttttggct atgatcctga
    44341 aagtgtcgga tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat
    44401 cctatctacg atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga
    44461 agcaattatc ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg
    44521 gatgagctta tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat
    44581 gtgcaaagaa taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt
    44641 ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg
    44701 attgtcaatg gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg
    44761 cggcttagta tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt
    44821 taaacatcat agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa
    44881 aacggcaact cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc
    44941 taatcttact tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa
    45001 acacatgaaa cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa
    45061 ccatcccgac ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga
    45121 aggaatcgat atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc
    45181 tactacttca caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa
    45241 agggtatgac acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac
    45301 attatttgcg atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat
    45361 cactagtatg ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca
    45421 gtcccttgat gaaatacttg cacatatcga tcgaatccga gataatacct acgcttatct
    45481 gattgtagat gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct
    45541 gattggcagc ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt
    45601 attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag
    45661 aagaaataac gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga
    45721 agtagcgatt gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat
    45781 tggtcatttc ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat
    45841 ttcagcgcaa taattccttt tattcaaaaa acataccaaa aatattccaa catcatcttg
    45901 gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt tatttctatc
    45961 aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg catatagttt
    46021 agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa
    46081 ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat
    46141 aaagttgatg gaagaaatgc attaaatacg gatgcaaaag ggctttcact gggccttgct
    46201 cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa
    46261 aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta
    46321 gctgcacaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca
    46381 gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat
    46441 accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac
    46501 ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat
    46561 taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa
    46621 accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga
    46681 caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa
    46741 tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca
    46801 aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg
    46861 acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc
    46921 aagaattact agaagctatt taccaaacag atacacttga agctgtactg acactgatcc
    46981 agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa
    47041 ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc
    47101 gagcgattga aaaaggatat ttattagaga ttcaagagcc gactgtcatc cgagatgcct
    47161 ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag
    47221 gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag
    47281 gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc
    47341 ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag
    47401 aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg
    47461 ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag
    47521 gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc actgattcag
    47581 atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg
    47641 acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa ggaagaacca
    47701 ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct
    47761 tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag
    47821 aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg
    47881 aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg
    47941 acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca
    48001 tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa
    48061 aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc
    48121 ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg
    48181 aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa
    48241 agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt
    48301 caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat
    48361 tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa
    48421 gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg
    48481 aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag
    48541 cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg
    48601 gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg
    48661 ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca
    48721 aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg
    48781 aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac
    48841 gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt
    48901 ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca
    48961 agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga
    49021 tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa
    49081 agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa
    49141 tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt
    49201 tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat
    49261 caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc
    49321 gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac
    49381 atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca
    49441 tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact
    49501 attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat
    49561 ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt
    49621 tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta
    49681 cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt
    49741 agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca
    49801 agaaaaagaa gggcaattag atgtccttaa attgaaactt gcattaagcg cttggcaaac
    49861 ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc
    49921 cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc
    49981 tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat
    50041 gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta
    50101 taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac
    50161 aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc
    50221 tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca
    50281 agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac
    50341 caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta
    50401 tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc
    50461 acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa
    50521 ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata
    50581 taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt
    50641 tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt
    50701 taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga
    50761 caggtacttg gacatgtttg atatgcatac cgattgatgt gtcaccgata tcgataccgg
    50821 cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag
    50881 aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt
    50941 ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta
    51001 gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt
    51061 ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa
    51121 caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca
    51181 attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa
    51241 tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt
    51301 ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat
    51361 aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct
    51421 aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg
    51481 tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga
    51541 ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag
    51601 atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt
    51661 aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc
    51721 atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat
    51781 cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt
    51841 aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc
    51901 gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata
    51961 gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt
    52021 tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag
    52081 agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg
    52141 aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt
    52201 gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg
    52261 aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt
    52321 cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat
    52381 aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc
    52441 aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc
    52501 gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt
    52561 ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta aaaaaacaaa
    52621 aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt
    52681 taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc
    52741 aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg
    52801 attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt
    52861 atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat
    52921 agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg
    52981 gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc
    53041 ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat
    53101 atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt
    53161 catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac tctctagtgt
    53221 atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa gaaataccgc
    53281 atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa
    53341 agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag cagtaaaacg
    53401 ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag cttccaacat
    53461 atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc ccatggacat
    53521 caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa aagcgattaa
    53581 tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc atcccataga
    53641 atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat gggtcatata
    53701 agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt gttccattaa
    53761 ataaagaata gg
//
LOCUS       DADXTH010000009        44836 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.009, whole genome shotgun sequence.
ACCESSION   DADXTH010000009 DADXTH010000000
VERSION     DADXTH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.009"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWI97_000518"
     CDS             212..604
                     /locus_tag="IWI97_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2462049.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWI97_000519"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWI97_000519"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBC2462050.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="IWI97_000520"
     CDS             3164..4774
                     /locus_tag="IWI97_000520"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBC2462051.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="IWI97_000521"
     CDS             5066..5935
                     /locus_tag="IWI97_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBC2462052.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="IWI97_000522"
     CDS             6100..7371
                     /locus_tag="IWI97_000522"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBC2462053.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="IWI97_000523"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="IWI97_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBC2462054.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="IWI97_000524"
     CDS             8762..9025
                     /locus_tag="IWI97_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBC2462055.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="IWI97_000525"
     CDS             9470..9919
                     /locus_tag="IWI97_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2462056.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="IWI97_000526"
     CDS             9976..11394
                     /locus_tag="IWI97_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2462057.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="IWI97_000527"
     CDS             11394..12254
                     /locus_tag="IWI97_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBC2462058.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="IWI97_000528"
     CDS             12428..13201
                     /locus_tag="IWI97_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBC2462059.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="IWI97_000529"
     CDS             13348..13713
                     /locus_tag="IWI97_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBC2462060.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="IWI97_000530"
     CDS             complement(13861..15069)
                     /locus_tag="IWI97_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2462061.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="IWI97_000531"
     CDS             complement(15088..15366)
                     /locus_tag="IWI97_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462062.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15520..16215
                     /locus_tag="IWI97_000532"
     CDS             15520..16215
                     /locus_tag="IWI97_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBC2462063.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16657..17454)
                     /locus_tag="IWI97_000533"
     CDS             complement(16657..17454)
                     /locus_tag="IWI97_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBC2462064.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="IWI97_000534"
     CDS             complement(17451..18635)
                     /locus_tag="IWI97_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBC2462065.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="IWI97_000535"
     CDS             complement(18660..19907)
                     /locus_tag="IWI97_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBC2462066.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="IWI97_000536"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="IWI97_000536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBC2462067.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="IWI97_000537"
     CDS             21887..22201
                     /locus_tag="IWI97_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBC2462068.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="IWI97_000538"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="IWI97_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBC2462069.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="IWI97_000539"
     CDS             22886..23530
                     /locus_tag="IWI97_000539"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBC2462070.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="IWI97_000540"
     CDS             23545..23874
                     /locus_tag="IWI97_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBC2462071.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="IWI97_000541"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="IWI97_000541"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBC2462072.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="IWI97_000542"
     CDS             24862..25686
                     /locus_tag="IWI97_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBC2462073.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="IWI97_000543"
     CDS             25679..26026
                     /locus_tag="IWI97_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBC2462074.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="IWI97_000544"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="IWI97_000544"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBC2462075.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="IWI97_000545"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="IWI97_000545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBC2462076.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="IWI97_000546"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="IWI97_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBC2462077.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="IWI97_000547"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="IWI97_000547"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBC2462078.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31656..32450
                     /locus_tag="IWI97_000548"
     CDS             31656..32450
                     /locus_tag="IWI97_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462079.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="IWI97_000549"
     CDS             32440..34482
                     /locus_tag="IWI97_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462080.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34763..34912
                     /locus_tag="IWI97_000550"
     CDS             34763..34912
                     /locus_tag="IWI97_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBC2462081.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34923..36437
                     /gene="dltA"
                     /locus_tag="IWI97_000551"
     CDS             34923..36437
                     /gene="dltA"
                     /locus_tag="IWI97_000551"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBC2462082.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36434..37639
                     /gene="dltB"
                     /locus_tag="IWI97_000552"
     CDS             36434..37639
                     /gene="dltB"
                     /locus_tag="IWI97_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBC2462083.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37684..37917
                     /gene="dltC"
                     /locus_tag="IWI97_000553"
     CDS             37684..37917
                     /gene="dltC"
                     /locus_tag="IWI97_000553"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBC2462084.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37920..39188
                     /gene="dltD"
                     /locus_tag="IWI97_000554"
     CDS             37920..39188
                     /gene="dltD"
                     /locus_tag="IWI97_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBC2462085.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39329..40411
                     /locus_tag="IWI97_000555"
     CDS             39329..40411
                     /locus_tag="IWI97_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBC2462086.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40411..41214
                     /locus_tag="IWI97_000556"
     CDS             40411..41214
                     /locus_tag="IWI97_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBC2462087.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41284..42024
                     /locus_tag="IWI97_000557"
     CDS             41284..42024
                     /locus_tag="IWI97_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBC2462088.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42035..43039
                     /locus_tag="IWI97_000558"
     CDS             42035..43039
                     /locus_tag="IWI97_000558"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBC2462089.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43084..44781)
                     /locus_tag="IWI97_000559"
     CDS             complement(43084..44781)
                     /locus_tag="IWI97_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462090.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14403 a         7826 c         9342 g        13265 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aatttcttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgagtgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggtatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagcacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgaat tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcgcaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa
    34621 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
    34681 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
    34741 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
    34801 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
    34861 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
    34921 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
    34981 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
    35041 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
    35101 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
    35161 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
    35221 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
    35281 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
    35341 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
    35401 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
    35461 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
    35521 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
    35581 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
    35641 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
    35701 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
    35761 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
    35821 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
    35881 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
    35941 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
    36001 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
    36061 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
    36121 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
    36181 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
    36241 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
    36301 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
    36361 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
    36421 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
    36481 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
    36541 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
    36601 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
    36661 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
    36721 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
    36781 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
    36841 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
    36901 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
    36961 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
    37021 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
    37081 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
    37141 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
    37201 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
    37261 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
    37321 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
    37381 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
    37441 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
    37501 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
    37561 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
    37621 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
    37681 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
    37741 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
    37801 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
    37861 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
    37921 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
    37981 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
    38041 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
    38101 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
    38161 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
    38221 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
    38281 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
    38341 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
    38401 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
    38461 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
    38521 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
    38581 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
    38641 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
    38701 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
    38761 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
    38821 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
    38881 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
    38941 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
    39001 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
    39061 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
    39121 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
    39181 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
    39241 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
    39301 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
    39361 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
    39421 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
    39481 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
    39541 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
    39601 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
    39661 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
    39721 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
    39781 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
    39841 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
    39901 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
    39961 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
    40021 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
    40081 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
    40141 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
    40201 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
    40261 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
    40321 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
    40381 aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat
    40441 cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc
    40501 ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg
    40561 gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca
    40621 ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt
    40681 aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg
    40741 atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc
    40801 agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc
    40861 atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt
    40921 tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc
    40981 gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat
    41041 agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca
    41101 atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa
    41161 ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta
    41221 agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt
    41281 acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca
    41341 acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg
    41401 ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat
    41461 tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca acttcatcct
    41521 aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt
    41581 gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta
    41641 aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc
    41701 atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat
    41761 ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt
    41821 tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg
    41881 aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa
    41941 caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca
    42001 gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca
    42061 cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga
    42121 acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac
    42181 gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt
    42241 aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc
    42301 tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact
    42361 tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg
    42421 catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac
    42481 aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa
    42541 aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct
    42601 tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat
    42661 tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa
    42721 aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc
    42781 tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt
    42841 gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg
    42901 agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc
    42961 aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt
    43021 gcttacctta ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat
    43081 tttttataca ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt
    43141 atctttttcc tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa
    43201 gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc
    43261 gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa
    43321 ataaattttt cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa
    43381 agcgtggatc aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc
    43441 tgcttggaag gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat
    43501 aagatttgtc acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc
    43561 taatatcttt tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag
    43621 attctccatc tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg
    43681 aagctgctca taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct
    43741 ccgatctttg tcggtattta agatgtgatc gattagattg tagctgataa taacgattag
    43801 aatgataata tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt
    43861 ttggatgtaa atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac
    43921 ataaaaacgg ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct
    43981 tataacacta gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt
    44041 tgcataaagc agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca
    44101 ttttttatag taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat
    44161 aataaataaa tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat
    44221 atcttctgat acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga
    44281 gtattcaaga cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc
    44341 gataaaaaca gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact
    44401 aatcaatggt ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata
    44461 aggagatgag actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac
    44521 agccaatgag agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt
    44581 ttcgcctaga tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc
    44641 caacggtttt tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat
    44701 cgtacgagtg tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca
    44761 cgaatctttt atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa
    44821 ataagcaatt cctgtt
//
LOCUS       DADXTH010000010        43771 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.010, whole genome shotgun sequence.
ACCESSION   DADXTH010000010 DADXTH010000000
VERSION     DADXTH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.010"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..900
                     /locus_tag="IWI97_000560"
     CDS             <1..900
                     /locus_tag="IWI97_000560"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2462091.1"
                     /translation="IRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYK
                     KQIILSHLHNNPLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVL
                     LPNTVNTERIIKMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVM
                     IGEGPLFDEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEA
                     MLLETPVVAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISA
                     KKFSNMNRYQGVLEKIYVSAALK"
     gene            904..2205
                     /locus_tag="IWI97_000561"
     CDS             904..2205
                     /locus_tag="IWI97_000561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBC2462092.1"
                     /translation="MNYKNRDQLVLRKIIPISILFQSGMIFYSLMPSIAIAVVSFFMF
                     LYYMWCLKKYFCLADSLLLTSILFIPTSTISILGTSYSSLPLTWHNLIIIILMLYIII
                     EGTLNRSYLIILFLFICFGISTSMFLPFLIDALKQLMTISLFICSFLIGKRFKMYSKN
                     LTMIGWNFYFIGVVSMAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAG
                     LYLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLF
                     NINRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYL
                     IGYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKW
                     LFLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
     gene            2216..2677
                     /locus_tag="IWI97_000562"
     CDS             2216..2677
                     /locus_tag="IWI97_000562"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HBC2462093.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            2736..4193
                     /locus_tag="IWI97_000563"
     CDS             2736..4193
                     /locus_tag="IWI97_000563"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBC2462094.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLL
                     SVCLCVILYFFIYSCIFKGKTEIINMVRMFTGKGY"
     gene            4228..5397
                     /locus_tag="IWI97_000564"
     CDS             4228..5397
                     /locus_tag="IWI97_000564"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2462095.1"
                     /translation="MKKVTILIPGMYYGGMERVAFITRQILLKNNYNVSFVTLFDNDA
                     DYQPNFEYLSLKCDIKPSKFGKVINIFKRVYKMKSVKKKLKTDITISFGQSCNLVNVL
                     SKRDDKVYVGIRSYDWLFQYIFHYYVDKFVYKKADAVVSVSQLIKRDAENIFNFNKDK
                     SYFLYNPYDLELINNKSKEKITEIEVPTDKKIIVTVGRLEDQKGLYHLIKAISMLKNK
                     DNVVVYILGHGTKKELLENLIKAYQLQNVIFLLGGQSNPYKYMSRADLYVMPSITEGF
                     PNALVEAMSTETAVLCADCKSGPREILSNRTLDTICENIEYAESGILVCPMTDSRNYS
                     PQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
     gene            5435..6526
                     /locus_tag="IWI97_000565"
     CDS             5435..6526
                     /locus_tag="IWI97_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBC2462096.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(6594..8072)
                     /locus_tag="IWI97_000566"
     CDS             complement(6594..8072)
                     /locus_tag="IWI97_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBC2462097.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(8338..9086)
                     /locus_tag="IWI97_000567"
                     /pseudo
     CDS             complement(8338..9086)
                     /locus_tag="IWI97_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            9229..10179
                     /gene="pfkB"
                     /locus_tag="IWI97_000568"
     CDS             9229..10179
                     /gene="pfkB"
                     /locus_tag="IWI97_000568"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBC2462098.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            10161..11624
                     /locus_tag="IWI97_000569"
     CDS             10161..11624
                     /locus_tag="IWI97_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2462099.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            11621..12079
                     /locus_tag="IWI97_000570"
     CDS             11621..12079
                     /locus_tag="IWI97_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2462100.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            12076..13050
                     /gene="lacD"
                     /locus_tag="IWI97_000571"
     CDS             12076..13050
                     /gene="lacD"
                     /locus_tag="IWI97_000571"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBC2462101.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(13229..14179)
                     /gene="yidC"
                     /locus_tag="IWI97_000572"
     CDS             complement(13229..14179)
                     /gene="yidC"
                     /locus_tag="IWI97_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBC2462102.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(14617..14892)
                     /locus_tag="IWI97_000573"
     CDS             complement(14617..14892)
                     /locus_tag="IWI97_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBC2462103.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            15000..15764
                     /locus_tag="IWI97_000574"
     CDS             15000..15764
                     /locus_tag="IWI97_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBC2462104.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            15887..16390
                     /locus_tag="IWI97_000575"
     CDS             15887..16390
                     /locus_tag="IWI97_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2462105.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            16798..17445
                     /locus_tag="IWI97_000576"
     CDS             16798..17445
                     /locus_tag="IWI97_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBC2462106.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            17608..18876
                     /locus_tag="IWI97_000577"
     CDS             17608..18876
                     /locus_tag="IWI97_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBC2462107.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(18903..20102)
                     /locus_tag="IWI97_000578"
     CDS             complement(18903..20102)
                     /locus_tag="IWI97_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBC2462108.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(20221..21528)
                     /locus_tag="IWI97_000579"
     CDS             complement(20221..21528)
                     /locus_tag="IWI97_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBC2462109.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            21999..23117
                     /locus_tag="IWI97_000580"
     CDS             21999..23117
                     /locus_tag="IWI97_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462110.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            23313..23456
                     /locus_tag="IWI97_000581"
     CDS             23313..23456
                     /locus_tag="IWI97_000581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462111.1"
                     /translation="MKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKNIIYEQNVNN
                     GRS"
     gene            23501..23821
                     /locus_tag="IWI97_000582"
     CDS             23501..23821
                     /locus_tag="IWI97_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462112.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            24273..24473
                     /locus_tag="IWI97_000583"
     CDS             24273..24473
                     /locus_tag="IWI97_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2462113.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            24620..27409
                     /locus_tag="IWI97_000584"
     CDS             24620..27409
                     /locus_tag="IWI97_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBC2462114.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            27456..27983
                     /locus_tag="IWI97_000585"
     CDS             27456..27983
                     /locus_tag="IWI97_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBC2462115.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            28004..29194
                     /locus_tag="IWI97_000586"
     CDS             28004..29194
                     /locus_tag="IWI97_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBC2462116.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            29234..30532
                     /gene="asnS"
                     /locus_tag="IWI97_000587"
     CDS             29234..30532
                     /gene="asnS"
                     /locus_tag="IWI97_000587"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBC2462117.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            31709..32383
                     /locus_tag="IWI97_000588"
     CDS             31709..32383
                     /locus_tag="IWI97_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBC2462118.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            32380..32721
                     /locus_tag="IWI97_000589"
     CDS             32380..32721
                     /locus_tag="IWI97_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462119.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            32751..33110
                     /locus_tag="IWI97_000590"
     CDS             32751..33110
                     /locus_tag="IWI97_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBC2462120.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            33634..35715
                     /gene="cadA"
                     /locus_tag="IWI97_000591"
     CDS             33634..35715
                     /gene="cadA"
                     /locus_tag="IWI97_000591"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBC2462121.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            36026..37492
                     /locus_tag="IWI97_000592"
     CDS             36026..37492
                     /locus_tag="IWI97_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2462122.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            38107..38646
                     /locus_tag="IWI97_000593"
     CDS             38107..38646
                     /locus_tag="IWI97_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBC2462123.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            38720..39160
                     /locus_tag="IWI97_000594"
     CDS             38720..39160
                     /locus_tag="IWI97_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBC2462124.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            39173..39382
                     /gene="copZ"
                     /locus_tag="IWI97_000595"
     CDS             39173..39382
                     /gene="copZ"
                     /locus_tag="IWI97_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBC2462125.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            39382..41568
                     /locus_tag="IWI97_000596"
     CDS             39382..41568
                     /locus_tag="IWI97_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBC2462126.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            41588..43759
                     /gene="copB"
                     /locus_tag="IWI97_000597"
     CDS             41588..43759
                     /gene="copB"
                     /locus_tag="IWI97_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBC2462127.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT        14312 a         7270 c         9119 g        13070 t
ORIGIN      
        1 attagaggtt taagttactt accattaaaa aaattttcga gaaaagaaat tgacagagcc
       61 gtacatgaat taaacccaga tataattcat gctcatgatt ttaatgctag tgtaagagca
      121 agtagatata agaagcaaat catcctttcc catttgcaca ataatccctt gtggttacct
      181 aaactagaca taagaactat tatatattct ttatgttcgt ctaggtttaa ttcagtaatt
      241 ggtgtttcga attccgttaa ggaagaattt cgttacaaac aattaataaa agataaattt
      301 gtgttgttac ctaacactgt aaatactgaa agaattataa aaatggggaa tgaatttgtt
      361 gaagatgata ttgatatttt atatgtagga cgcatggcag aacctaaaaa tccaataggt
      421 gcaattaatg ttttagaaaa tgtagtaaaa aaatattcaa aacatttacg tgtcgtgatg
      481 attggtgaag gaccattatt tgatgaagtt aagatgcaaa tacaaaaaag aaacttaaca
      541 gatgttattg agttaaaggg attcaaaagt aatccctata agtacatgaa gaaatctaaa
      601 gttctattaa tgccatctct ttgggaaggg tttggcctag tatcagtaga ggcaatgttg
      661 ttagagacgc ctgttgtagc ttataacgta ggaggattga aggatattat cgatgagaca
      721 aatggatttc tgtgtggtag tgaagttgaa ttatctaatt cactaatgaa gctattaaat
      781 tcagaagaac ttagaaaaaa aatgggagaa aatgctgcta ttagtgcaaa gaaattttct
      841 aatatgaatc gttatcaagg agtattagaa aaaatttatg tatctgcagc tttaaaataa
      901 aatatgaatt ataaaaatag agatcaatta gttttacgaa aaataatacc cattagtatt
      961 ttgtttcaat caggaatgat tttttattca ctgatgccct caatagcaat tgcagttgtt
     1021 tcttttttta tgtttttgta ttatatgtgg tgcttaaaaa agtatttttg tttagcagat
     1081 agcttattgt taacatcaat tcttttcatt ccaacatcaa ctatatctat attaggaact
     1141 agttattcta gcttaccatt gacgtggcat aatctaataa taataatttt gatgttatat
     1201 attattatag aaggaacatt aaatcgttca tacttgatta ttttattttt atttatttgt
     1261 tttggaatat cgacgtctat gttcttacct tttttaatag atgcattaaa gcaattaatg
     1321 actatttcgt tgtttatctg tagtttttta ataggaaaac gttttaaaat gtatagcaaa
     1381 aacttaacta tgattgggtg gaatttctat ttcataggag tagtatctat ggcgtttcag
     1441 gtatatttgc aacgagtata tattttatct actggtgtaa taataggcca ttatgcagaa
     1501 atgggtttaa atagaattgc atatgcaggt ttgatgggag attttagttt tgcagggtta
     1561 tatcttgcta caggctgtct atatgctttt ctaaaatatg tggatttaaa aaattggaaa
     1621 ttattattct ttataataat agaagtattt ttattaggtg caattttatg tgttagtgca
     1681 agaacagggt tagtttcctt gtttgtcaca atgacattgt attatctatt taatataaat
     1741 agaattagtg ttcctcatct tttagttcta ataggtggaa tcataactac accttatata
     1801 ctagtaatgt taatggcagg tcgttctgga caaagtcttt tggattcttc aggcagatta
     1861 gaaaactata tgtcctcttt aaatgctttt agtgataaat acttaatagg atatggatta
     1921 ggattaaata atttatacac gctactaggt gtagcagtac cacataattt ttttattcaa
     1981 tatttattac agacaggaat tattggtacg ctaattattt taagtggctt ctttaaattt
     2041 acgtatgaag aattaaagaa atcaggttgc tataaatggt tatttttgat gatagtagtg
     2101 ggagcaatgt ttataccgga tattgtatct tcgcgttttc tttacgcaat agttgttttg
     2161 tgtatgattt ctgcatcaga aagaactata aaaaaagaaa gttgaggtag aaaaaatgag
     2221 aagaaaagca gagaaatggg cagataaagc ttgtagacaa tacaggaatg gtcaaaataa
     2281 aatgtcaaaa atatcctgct atattttaag gattatttat ggatgtgaat tctacggaca
     2341 ggtagatttt agctctaatg ctcaactagt ccataatggt cttggttgtg tattccaccc
     2401 taaaacaaaa atcggtaata attgtaaaat atatcaaaat gtgactttgg gtggaaatgg
     2461 caaaataata aatggaaaag aaactgttgg ggcaccaacg ctagaggaaa atgtggctgt
     2521 tttcgctggt gcatgtgttt taggaccaat taatatcgga catgattcaa tcataggtgc
     2581 aaatgctgta gttacaaaaa gtgttccacc atatagctta gtatatggaa atccagcaat
     2641 cataacagag ttgaaatttg attataaatt tgattaaaaa gtaatgttta atatgcgaaa
     2701 ggttgctata tttatttagc aaaatggggt aataaatggg aaataatatt catggaaaga
     2761 cagcaaaagc tttaaaatgg tcatccttag ctgaaataat tgcaaagatt ataactccaa
     2821 tttcgaatat gattttagct agattattga ctccagatgc ttttggagta gtagccactg
     2881 taactatggt tattagtttc gctgatatgt ttagtgattc aggatttcaa aaatttttag
     2941 tacagcatga atttgataat gaagaacaca tgttttcatc aacaaatgta gcttttacta
     3001 gtaacttagg tatttcttta ttattatggt tatttatttg tgtgtttaat aataaaatag
     3061 cgcacttagt tggaaattct ggacttggat tagtaattat agtagctgga gtttctcttc
     3121 caattacttc tttttccagt atacaatcgg caatttttag acgaaatttt gattacaaaa
     3181 ttttgtgtta tgctagaata atatctgcat tgataccttt agttattaca gtaccgttgg
     3241 cgataattgg actaagttat tggtcactag taattggatc tataatagga aatttagtta
     3301 cagctgcaat attaactaag ttatcagagt ggaaaccaaa actttttttt tcaaaaaaag
     3361 aattttacga aatgttttcc tttagtgcgt ggtcactagt ggaatcatta ggtacatggt
     3421 tgtcatctta tataggtact tttatcgtag gaggcatttt aagcgtatac tatttgggaa
     3481 tatacaaaac tacaatgaca acggttaatg gtatttttgc aattgtaact tcagcaacaa
     3541 caactgtatt attttcaaca ttatctcgtt tacaaaatag taaaacggag tatgataaga
     3601 catatttaga ttttattaaa attgtttcca tttttattat tcctctgggt ataggtatat
     3661 atgtatatag ggaactagta acaaatattc tactcgggag tcaatggaaa gaggctgtac
     3721 catttgttgg gatatatgga ttaatgagct gttttactct tgtcttggga caatatgcct
     3781 ctgagtacta tcgtggttta ggaaaaccaa aagcaaatgt tcttatgact atcctacatt
     3841 tagcagtatt aattccagtt ctaataatct attcaaggaa agggtttata gaactagcat
     3901 atggtagatc tttcgttaag atagagcaga ttatagtctt ttggattatt ttatggtttg
     3961 gttttaagtt taatccattt aaaattttaa agcaggtttg gaaatcttgt gctagtgcat
     4021 tgctaatggg agttctagca aaaattatgt tggaattctg tactaattat gttatgcaat
     4081 tactttcagt gtgtctttgt gtaattctat atttttttat atatagttgt atatttaaag
     4141 gaaaaacaga aataattaat atggttagaa tgtttactgg taaaggatat taagagagat
     4201 ttttatggca aggttgtgat taggaaaatg aaaaaagtaa caattttaat tcctggaatg
     4261 tattatggtg gaatggaaag agtagctttt ataacgcgtc aaatactttt gaaaaataat
     4321 tataatgtat catttgtaac tctttttgat aacgatgcag attatcagcc aaattttgaa
     4381 tatttatcat taaaatgtga tataaagcca tcaaaatttg gtaaagtaat taacattttt
     4441 aaaagagttt ataaaatgaa aagtgtcaaa aaaaaattga aaacggatat aactatatct
     4501 tttggacaaa gttgtaattt agtaaatgtt ttatcaaaga gagatgataa agtatatgtt
     4561 ggaatccggt cctatgactg gctatttcaa tatatattcc attactatgt agataaattt
     4621 gtatataaga aagcagatgc tgttgtgtct gtcagtcagt taattaaacg tgatgctgaa
     4681 aatattttca attttaataa ggataaaagc tattttttat ataatccata tgatttagag
     4741 ttgataaata ataagagtaa agaaaaaatt acagaaatag aggttccaac agacaaaaaa
     4801 attatagtga cggttggtcg attagaagat caaaaaggtt tatatcatct aattaaagct
     4861 atatctatgt taaagaacaa agataatgtt gttgtgtaca tcttaggaca tgggacaaaa
     4921 aaagaattat tagaaaattt aataaaagca tatcaactac aaaacgtaat atttttgcta
     4981 ggtggccaat caaatcctta taaatacatg tcacgtgcag atttatatgt catgccatct
     5041 ataactgaag gttttcctaa tgcattagtt gaagcaatgt ctacagaaac tgctgtctta
     5101 tgtgcagatt gcaaatctgg gccaagagaa atattatcga atagaacgct agacacgatt
     5161 tgtgaaaata tagaatatgc tgaatcaggc atattagtat gtccaatgac agacagtaga
     5221 aattattcgc cccaatatga agaatgtgat aaaaatttag cgaatgctat agaagaattg
     5281 attgaaaacg ataatttgag gaataagtat gcaaaagcag caaaaaagag agcagaacta
     5341 tatagttatg atgcctttga acataacttg ttgcaaatta tgaagacatt taattaattc
     5401 atatgatgaa ataaaaataa gggagtataa ctagatgctg aaaaaaaata catctttaca
     5461 gaataaaaca atattaataa ctggcgcagc cggttttatt ggatcaaatt tggttcttga
     5521 gttattacgt actcagtcat caataaaaat cattggcatt gacaatatga atgattacta
     5581 tgatgtctct attaagaact atagattatc agagatagaa acggaggcaa agaaccatcc
     5641 aggatctact tggaatttta tcaaaggttc tattgctgat aaagcaacaa ttgatttcct
     5701 ctttgataat tataaaccaa gtatcgtggt gaatttagca gctcaggcag gagtccgtta
     5761 ttcaattact aatccagata tgtatatcga atctaacata ataggattct ataatattct
     5821 tgaagcttgt cgtcattcat atgataatgg agaatctggt gtggaacatt tagtgtatgc
     5881 ctcatcgtct tccgtttatg gtactaataa gaaaattcct tattctacag aggataaagt
     5941 agacaatcaa gtatcgttgt atgcagcaac taagaaaagt aatgaatcaa tggcacatgc
     6001 atattctaaa ctttacaata ttccgtctac agggctacgt ttctttacag tatatggtcc
     6061 tgctggtcgt ccagatatgg cttattttgg ttttactgat aaacttcgtg ctggtgataa
     6121 tatccaaata ttcaattatg ggaattgcaa gcgtgacttt acctatatag atgatattgt
     6181 tgaaggagta aagagggtta tgcagtgcgc tccagagaaa attgatgggg aagatgggct
     6241 accaatccca ccatatcata tttataatat tggtaacaat acgccggaaa acctactaga
     6301 ttttgtagat attctacaac aagagctaat tgcagcaggg gtgctaccag aagattacga
     6361 ctttgaatct cataccgaac tagttccaat gcaaccagga gatgtgccag ttacttatgc
     6421 agatactagt gcgttagaac gagattttga atttaaacca agtacaagtt tgcgtgacgg
     6481 gcttcgtaaa tttgcgcagt ggtataagtc tttttatgaa gtataattgt atttttttac
     6541 ttataaaagt ttatagaaac acctcagaca ctacaaatct gaggtgttat tttttaatct
     6601 cgttgaaata aatctctggt atatactttt tcttttacat catcaagatt actatcgtaa
     6661 cgattagcaa taatactgtg actgatattt ttaaaatcat ctaaattatt tattactttt
     6721 gaaccaaaaa atgtagtgtt attttcaagt gttggttcat aaattacaat agttgccccc
     6781 tttgctttaa ttcgcttcat aactccttga atagatgact gacggaagtt gtctgaatta
     6841 ctcttcattg taagacggta tacacctatg actacctctt tttctttttc tgcattccac
     6901 gaactgttag cctcatacgt gccagcaagt tctaacactc gatctgctat aaagtctttt
     6961 cttgtacgat tactatcgac aattgcactt atcatattct gaggaacatc gttatagtta
     7021 gtgagaagtt gttttgtatc tttcggtaaa caatagcctc catatccaaa agatggatta
     7081 ttataatgtg tgccaattct tggatctaag caaaccccgt gaatgatttc ttgtgtattt
     7141 agtcctttca tttctgcata agtatccagc tcattgaagt aagatactcg aagtgcaagg
     7201 taagtattag cgaataattt aactgcttca gcttctgtga atcccatgaa aagagtttcg
     7261 atatcttttt tgattgctcc ttcttggagt aaatctgcaa aagtgtgaga ggcttctcgt
     7321 aaagttttgt cagtttggtc cgtgccgaca ataattcgcg aaggatatag attgtcatat
     7381 aatgccttag attctctgag aaattctggg ctgaatagaa tatttttagt attgtatttt
     7441 ttacgaatac tttcggtata gcctacgggt accgtcgact tgatgaccat tattgccttt
     7501 ggattgactt ctaacaccaa tttgattact gactctacag cagaagtatc aaaataattt
     7561 tgtttactat cataatttgt tggagcagca atgacaacaa aatcggcttc tctataggcg
     7621 gctgcaccat cagtcgttgc aattaaatta agattttttt cagctagata tttttcaatg
     7681 taatcgtctt gtattggaga tttcttattg ttaatttgtt ttactttttc aggaagaatg
     7741 tctactgctg ttacctgatt gtgctgggca agaagagtag caatggaaag acctacgtaa
     7801 ccagtaccgg ctacagcaat attcttcgac tgatgttttg ttctgtttga tttatttgat
     7861 tcatttataa atagatctgt tggttcaaaa tttagaactt ctgctaatga ttctaattgt
     7921 tgtattgacg ggataaaatc gagagattct actcgactaa ttaaggcacg gttgatacca
     7981 gtagctactg caagttgttg ttgtgtcatt ttcagagatt ttctagaatc aataatgatt
     8041 tgagctaaac gtttttgaga taactttttc atgatgcact cctttttttg ttgctaataa
     8101 taacaaacaa ttttttcttc tataacagaa tagcttatta ttaataaaac tgtcaatttt
     8161 tttattgatt aaaaagatat tttttgattt taagtatttt caattatttt ttgttactta
     8221 aaataacttt atagattgtg aataacttta tacataaaaa gtttacgtga gtccatacaa
     8281 tataaagaat aaaaaatgtg atgttttaaa ctgtaaccaa ccataataaa ctgcttctca
     8341 attctccctc atacgcaata cataggcgtc aagcaggatt ctcgcgatga tctggagagg
     8401 caagcgtgaa cgtacttcat aatcagggaa ataagaacct tctgatttga aacctacgag
     8461 atttatctcg caataatctt tgagtgtaga ggaatgattg gctgtaacta gaatcgaacc
     8521 aatctctgca tcatagcagt tctttgcggc ggtgacgagt tcttttgtct cgccgttcaa
     8581 agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt tgataatctc
     8641 tggatcagta tgcatttgac aattttttcc tgttaattga agttttacga gcatctcttg
     8701 accaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagtaa atttgattct
     8761 ttgtattgca tcttcaatgt tcccgatttc caacatattg atcgtacgta ccacttcttg
     8821 ttcatttttt agaatggctc gacggatacc tttgtctact ttatctaaaa agaaaaattg
     8881 atgttcttgt tttctccttc ttttagatgg tgcttaaacg aagtaaatcc ttcatagcct
     8941 tttttcttca ttgtccgcac aatggtggct gttgagacat ttgcttgttc acttagtttc
     9001 acgatggagt agttagggat ctctaggatg tggtgttcga taaagttcca gaggtactgt
     9061 tctgtttcac ttaatttgtg atccatattc tttccttctt tcactaaaag ttagttaaaa
     9121 aatagcacga tgtgtttata aatgtaaagg ttttctttgt gttggaacga ttaagaaaac
     9181 attttctctt tcgcgtattt cataatagat tcaaggaggt cattggttat gtctatctac
     9241 acatgcacga tgaatttggc aattgatcta tttattgaaa cagaggagat gcatcctttt
     9301 atggtaaacc gtacaaagga ggatgacatt caggcaaacg gaaaaggagt aaatgtgtca
     9361 ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc tggtttcaca
     9421 ggaaaatata tagaagacta tttacagaag aaacaaatcc aaacagagtt tattgaagtg
     9481 cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgagaga agaatacaaa
     9541 ttagtgaatc aaggacctga aataccgcag gcagctgtcc ataatttttt aaatcaaata
     9601 agagcattac aagcagagga ttatctttgt gtttctggga gtttaccaag aggaatgtct
     9661 ccgaaggttt tagtggaaat cagtcggatt tgtcaggaaa aggagattca tttgattatt
     9721 gatagcagtg atcaagaggt catggattgt ttgccctatc gaccattttt gctgaaaccg
     9781 aatgaggaag aactttcttc ttggttcgga cgaaaaatgg ttacagatga agattacttt
     9841 gtttatggac aacgactggt agagtcaggt gcagaaaatg ttttgttatc ccttggtgag
     9901 aaaggagcga ttctttttac gaaagacagg gtgattcgag gaaactcgcc aaaaggaaaa
     9961 gttgtcaata cagcatgtgc aggtgatgcc atgttaggca cttttttagt aggatacatg
    10021 aatcgacgac cgcttgatga aacgttacgt aaaagcatag cagcaggcag ctctactgct
    10081 tttcgtaaag gactgactga ttttttggat gtggaggaat taagtaaaca aatcaaaatc
    10141 aaggaagaga ggttcgaaag atgggaaaat atcaactaat tgcagcaacc ggttgtccga
    10201 caggaattgc acacacctac atggcgcaag aggcattaga gcaagcagct agaaaaaaag
    10261 gcatcacgat caaagtggag acccatggac aaattgggat cgagaatgaa ttaacggctg
    10321 aagaaatcaa acaggcagat gctgtcatta ttgccgcaga caaagatgtc cattcagaaa
    10381 gatttgcggg aaaacgagtc attgatgttt ctgtcagtac aggaatcaag gatgcacaac
    10441 ggctgattga cgaagcattg gcaggaaaag ggaccatctt agcagataat gaagctgttg
    10501 atactttaga atcagaaacg ataagtggtg ctaattttgg tcgcagcatc tataaaaatt
    10561 taatgaatgg cgtctctcat atgttgcctt tcgtggtagc tggcggagta ttgattgcta
    10621 tttcatttgc cgtttgggga atctattctt ttgatccaga ccatgcacaa tacaatgcta
    10681 cagcggcgat gttaaaaacg gttggcgatg cagcaatggg gatgatggtt ccagttttag
    10741 cagcttatat tgcagaaggg atcgcgaaac gtccagggtt agtcgttgga tttgttggtg
    10801 gaatcgtggc catgagtggc ggtactggat ttttagtagg tattttagct gggttcttag
    10861 caggttattt tatcttattt ttgcaaaagc tactgacaat tttacctaaa caactagatg
    10921 gcttgaaggc aatcttctta tatccagtta ttggtgtagt gtttatcggc atcgtgatgt
    10981 ctttgctggc gggtccaatg aaggctataa atgaaggaat gatgagcttt ttagctggat
    11041 ttgaacattc gaatccactt gttcttggcg tgattgttgg ttgcatgtgt gcgtttgata
    11101 tgggaggacc gattaataag gcggcatatg taacaggtac agctcttttg gcacaaggaa
    11161 acacgacttt tatggctggc gtgtcagtag cttgcattgc accaccgttg attactggtt
    11221 ttgcaacttt attgtttggt aaatattttg atactaatga tcgtaatgca ggtttggtta
    11281 attttatttt aggatcaact catattacgg agggagctat tccgtttgct gcgaaagacc
    11341 cgatccgagt attaccaacg atgatgttag gctcatcaat tgctgctgtc ttgacctatc
    11401 tgtttggtgt ccaagtacca gccccacatg gcggtttttt agtgttacct gtagtgacgg
    11461 gaaaaatcca atgggttaca gcgattctta ttggttcgat cgttggtgga gtgatactag
    11521 gtttgatcca aaagaaaaga gtaactgata aagacaagaa gatggataat aaagcagcaa
    11581 atcgtacagt tatcgaagaa ggaagagcag gaggcttatc atgacaatca ttcatgaaga
    11641 gaacattttt ttacaacagc cattagatag tcaagaagaa gcatttaagt ttttagcaaa
    11701 gcaaacattc tgtttaggaa tcgctcatga cgaaaaagaa gtatatcgaa aacttctaga
    11761 aagagaaaaa gaaggtacta caggaataat gtcaggtttt gctatccctc atgcgaagtc
    11821 tcaaacaatc aacgaaccta gtatgattat cgtcacactg aacaaaggaa ttgaatggaa
    11881 cagtttagat agacagtcag ttgattttat ttttgcgtta tttattccag attcagaagc
    11941 aggaacgact catttgaaat tattgtctct cgttgcacga tcattgatgc gaaaagaggt
    12001 tacagaacag ttgagaaaag caaaatcaaa aacagaaata gccacaatat taactaaaga
    12061 aataggtgaa gatctatgaa gaagatgagt atagaaaagc aggaacgttt gaagcgattg
    12121 acggatgaag ctggaattat tagcgcgtta gcaatcgacc agcgtggtgc tttgaagaaa
    12181 atgatcaacc aatataaaga agctacatca gaagacatca tccaatttaa acagatggtt
    12241 tctcaatacc tgactcccta tgcctcagct attttacttg atccagaatg cggcctgcct
    12301 gcagcagcgc ttagagagaa agaatgtggc ttgctgctag cttatgaaaa aaccggctat
    12361 gatacgaaaa tgccgggaag attacctgat ttattgaata tttggtcggt gaaaaggtta
    12421 aaagaagctg gtgcggatgc ttgtaaattt ttattgtact atgacgtgga tgaaccagca
    12481 gagattaatg tgcaaaaaca aatagttata gaacggattg gttcagaatg cgatgcagaa
    12541 ggcatacctt tctttttaga gatcctttct tatgatgcaa ctattggcga tccgtcctct
    12601 cttgcctatg caaaagtaaa accccataaa gttattgaag ccatgcgaga attttcaaaa
    12661 gaacgttatc aagtggatgt attgaaagta gaagtgccag tgaatatgag atttgtggaa
    12721 ggatatgggc aagagacttg ttatagtcaa gcagaggcat ccgcgtattt ctatgaacaa
    12781 tcgaaagtga ctgaacaacc gtttattttt ttaagtgcgg gagtcaatgc tacgttattt
    12841 caagatacat tacgttttgc aaaacaatca ggttcgacat tcaatggtgt cttatgtggg
    12901 cgagcaacat gggcgaaggg agtggcacct tttgttaccg aaggcgaaga aaaagcgagt
    12961 agttggatgt tagaagaagg aaaacaaaat attaaggaat taagtacact attaaaagaa
    13021 acagcaacac ctgtctacgt tgatagataa gaaaaagttg tttcttcata aaaagatgct
    13081 gctgcaatta gatagcgatc aatcgatgat tcaaattata cattcactta aataaaaata
    13141 tcgactagat ttctttttca aaaatcttgg tcgatatttt taattgctaa aactgttaat
    13201 gaatttttat tacactctta tttactatct acttccgctt ctgctgcttt cctgcgttgc
    13261 ggcctttgtt atttgtactg ttttttggtt gtttgatttg ttttgttgct tgtttgttgt
    13321 cagctggttt tgctgattct gtcacgtctt ttcttggagt gacgacttgt tttggcgggt
    13381 tttgtttcaa ctcttctgcc acttgtgctt tcaagcgtgg tttcatcata acgtttgtga
    13441 tgaatgtttg cagacagctg aacacaccac cgactaccca gtaaagtgta acgccggctg
    13501 gcgcactgat cgacatgaag acgatcatag caggagagac gatcagcatc gtacgcatcg
    13561 tctttttctg ttcttcagga ataccgattg ttgaaatata tccttgaagc agataagcga
    13621 cccctgcaat ggccacaagc accatacttg gtgagcctag atcgatgccg taaaaagatg
    13681 attcacggat accttctgtg tagcgagccg taaaatacaa ggcagagaag atcggcattt
    13741 ggatcaatag aggcagacag ccgattccgc cggtcatact caatccgttt tcgcgatata
    13801 cttgctgcat ttctgcttgt gctgccattt gttcttcacg tgttgttgca gcttttagct
    13861 tttgttgtgc tgcttcaact tgtggtttta acgcttgcat tttttcagat tggatcattg
    13921 tttttttcga ttgactgata cctagtggca agatgatgat acgaacgatc acagtcattg
    13981 caataactgc ccagccgtaa gaccaattga aattctcaac tagatatgta atcgcttggc
    14041 ccataggatg gacgagtact cgataaacga ttcctgaagt gtcagggttt ccgtttgaat
    14101 ccatccgcac acagccagat aaaaagagca ggatactgac aagtccggat cccaataacc
    14161 agtttttcca tttattcata gaatacttcc tcttctataa tttttacaca aacttaacta
    14221 tactaaaaaa gcgcatggaa ttcaatcaac ttcatagaaa tttcatataa atattctttc
    14281 tttttcgtag cgaaaaattt tgaaaaaaga caaaaaatca agaaagaaat acttggaaag
    14341 aaaagagtga ttccaagtgt ttctttctta tataaaaaac taaaaacttt tttgttctgt
    14401 ctggaaaata tgggttaagc aaatttattg caagttatag atcagctttt ttactaccta
    14461 tctttaatac ttgaggcaaa gcgcctcaaa aacaacctga tcgacggtgt tcaaaagtca
    14521 gtttctcggt attaagtcaa atttatgcaa aaatgtggaa agccattttc acaaaatttt
    14581 ccttaatact caaaactaag cgaccttttc acaaccttat cccaccacat caaatttctt
    14641 tcgttcctca atcatcggat cttcttggac atccacatac tgcactcgtc cacttgggct
    14701 cggagacttc ttgacttctt cgataaattt ttggatgatg tcgtcatttc cgattgcttc
    14761 gattgtgact gagccgtcgt cttcgttttt taccgttccg gtcacgccta actgatcagc
    14821 aaccatcttg gtcatatagc gaaatcccac gccctggaca cgtccttgga cattcatttt
    14881 aactttgcgc atgttcgtca cctttctttc ctgttttata aatcaatgat accgattttt
    14941 ttaggatgaa acaagctcaa gcacctattc catgatataa tcaggtgagg tgaaggaatg
    15001 tgaaagagat ccaatcagca aaaaatccaa aaatcaaaga attaaaaaag ctacataaaa
    15061 agaaatacag agaagaaaag gcagaatatc ttttggaagg gttccatctg atcgaagaag
    15121 ctgcgtcgtc tgatgcagaa ataaaagaac tttttgtgac acagagaggt atgaaagaat
    15181 ggggagactg gctggctgta aacaaagaga tttctacttt tctagtttca gatgaggtca
    15241 tgaccgctat gtcagatcta cctacaccgc aagggatctt agcggtgatg acgttaacat
    15301 ctgaagatac gccagatttt tcaggaggct ggcttttact agataatgta caggatcctg
    15361 gaaatgtggg aacgatgatc cgtacggcag atgcgtttgg tatggctggt gttttattag
    15421 gtaaaggaac ggcagatatt tatagtacaa aggtgctgcg aagcatgcag ggaagcaatt
    15481 atcatttgcc tgtgttacaa aaagatttgg cagaagtgat ccctgtaatg aaaggcaaag
    15541 ggtttacggt gtacggtaca gaattaaatg aagaagcaca tgcattagac aaagtagaaa
    15601 aaacagagga tttcgctata atcatgggaa acgaaggtca gggagtaagt caggaaatct
    15661 tgtctttgac tgatcagaac gtatatatcc caatgaaagg aaatgcggaa tcattgaatg
    15721 tcggagtcgc agcagggatc gttctttatc atttttcttt atgattaagg aactattaag
    15781 ctttagctat tttatctaga gcgactagaa taatccgaac actggtggta tacttagatc
    15841 aatttcagaa gaaaatgaaa ttcaaaaagg tggcagatcg ataattatga cagaaaaatg
    15901 gcgtgaagac gaagaatata tgtcttatgt acaagatctt ttagaaacag aagcagtgaa
    15961 aaaattagcg aattacacac agcatgttca ttccactcgt ttggagcatt ccatcagtgt
    16021 ttcttactat agttatctgc tcgcaaaaaa atggggtggc aatgctaaag caacagcgcg
    16081 tgctggactt ttgcatgatc tgttctacta tgattggcgt acgacgaaat ttgacgaggg
    16141 gacacatgcg tatatccatc caagaattgc tgtaaagaat gcagaaaaaa tcaccgattt
    16201 gtcggatctt gagcgagata tcattttgaa gcacatgtgg ggtgcaacga tcgcaccgcc
    16261 taagtataaa gaaggatata ttgtgacatt cgtggacaaa tactgcgcag tcaaagaagc
    16321 ggctcagccc atgtctgcaa gtatgcgtaa gagatggcaa cgttactttg ggaaagaatc
    16381 ctcgatttaa caggtaaaac caataataga ccatattgag gttgtgacag aagtggtcag
    16441 cttcaagaaa taagaaggaa tttacgaaaa ttacttctca aatttttgtg aagtttcggc
    16501 ttattaccgc agatgcaagc ttttgaacac cattcattcg aaacaaggga ctttgacacg
    16561 acttttgtca cagtcctttt ttctgtttgc agttgactat ctgttcaaga tacggtttac
    16621 ttttatttat cgaatgaatc atgtacatag aagaataagc attaaagggg gtgatgataa
    16681 gatgcaaaac gtatcttatt taaaagagtc cgggatcttc aggtaacaca tactttgcag
    16741 aagatcactt ttttctacaa agtatgcact ttggttagaa gaaaggactt taaaatcatg
    16801 gaacaaacaa taaaaccata ccaatatttt tttataaaaa gagaagtaga acagttatta
    16861 aatgcgtact catctgtcaa tgatccaaaa acggtacaaa cagttcaggc actagcaact
    16921 gaaaagattc gagacatatt agaacacgaa ctgccagaaa tcgatacatt tttaacgaca
    16981 gttttagacg taaaactgac aaaagcgcaa gcagaacgag cattggagga gctgaagcaa
    17041 atcgttcagc cgtttatgca gcctagcaaa ccacaaatcg agaaattatt ccgaaaagtg
    17101 aaaaaactca aacaaccagc ttggaatgaa atcgacttgc aagaacatac gtacatcggc
    17161 tggaatgatc caggtacgca gaaaaaattc atgatctatt acgaagatga aaaattaaaa
    17221 gggattagtg gtactttatc accaacgata aataagggaa tctgtgcaat ctgtcagaaa
    17281 acatcgaatg tttcgctttt tctttctaca acgaaagcag gttctgatgg cacgtatacg
    17341 aaaaagggga attatatttg tcatgatagt gaccaatgta atcaacagtt aacgaaattg
    17401 gctcctttct atgaatttgc tgaacgtgta aagaaagaac gataatttta ttgtttttta
    17461 cagaaaaata tgctatcttt taatggaagt gaataggtga tggtgttcgc ctttaaatgc
    17521 taacgaaaga tagctaatga cgcctactaa agtctgggga cagccccttt ctttagtagg
    17581 cgttttttta ttgaggagga attttttatg gaaaaacaat taccgagtcc agtacgcatt
    17641 ttagtgtaca tgctgaagtg gctggtgatc acagcgattc tcggtatatt catggggagc
    17701 ttgtctgctt ttttcttaaa tagcctgaca gttgtgacag atatccgact atcacatcct
    17761 tggctccttt atctactacc agttagtggt gcgctttttg cttatctcta tgcgtatcac
    17821 ggcggtcttt ctagtcgtgg aaacaactta gtgatcgatc aaggaaatgg tggagaagaa
    17881 aaaatcccgt tgcgacttat tccacttaca ctgtttggaa ccataactac ccatttattt
    17941 gggggatctg ttggccgtga aggaacagca gtccaaatgg ggggagcgtt agcagataat
    18001 acagcacgtt tgttccgatt ggataaatca gaacgggaaa tcttgattat cagtggaatc
    18061 agtgcgggtt ttagttctgt atttggcaca cctttagctg gaacattatt tggattagaa
    18121 gtgttggcaa tcgggaaagt ccgaacagaa gcgatcttcc ctagcttctt tgctgcactg
    18181 tttgcaaatt tcatgacaga aagctatggt gtttctcatc tacattacca aatgggtgtg
    18241 attcctgaat ggagtatgtt gctgttcttc aaagtttttg ttgctgggat tgcttttggt
    18301 ttgattggtt gggtattcag ccgttcgatc gtctttttaa aagcgcgata tgcacaatgg
    18361 attcctcatc ctgtttggcg aaatacgata ggtggagctg tagttgtggc agcagcattg
    18421 attttgcaaa cacaacgtta tctcggattg agtcttccgt tacttcaaga atcatttaat
    18481 ggacaggcac atgtctatga ttttattggg aagttattct tcacagtttt gtctttaggc
    18541 gcgggatatc aagggggaga agtgacacca ttgtttgaaa ttggtgcaac gcttggtgca
    18601 gcactagcgc ctttgctcca tgtttctgtt ccttttcttg caggattggg ctttatcgga
    18661 gtattttctg gtgcgacaaa tacaccgatt gcctgctttg tgatggggat cgaattgttc
    18721 ggttcacaag cggcagtata cttctttatg atctgcttga tcagctttat gtgttcaggc
    18781 aacagcggta tttattctgc tcagcaagta gcggtcaaaa aagggatact ctttttgcca
    18841 atagttaaga gaatgaaaga aaaaaacaag gtgtgacata tgttttgtca caccttgttt
    18901 ttttagaaaa gtaaagtaat cagcgataag aatgcggagc ctgcgaaaag caatccgcaa
    18961 atgatcatat gtcttttttt gttcatcatc aagatggcga tgtattctct taaaaacgca
    19021 ttaggcaagt agtagaaagc tgtttttgca ccaataccat ttgctttcaa tttggcttgc
    19081 cacgcataaa gaccggctct aaaaatgtgg taattgttgg aactaaagat cgcgcggtat
    19141 ggccctttca tttgctgatc catgatttcc tttgaataga gcatgttctc caaagtggtt
    19201 gtcgaattgg tttcgacaag aatgtctctt tcagggattc cttgttccat ggcatattgt
    19261 ttcatcgcaa tggcctctgg tactttttcg tctgctcctt gcccgccaga catcaataag
    19321 atgggcggat ttaatgtagc acggctttga gcccgataaa aagcaattgc cttattgatt
    19381 cgcttggcta gaagaggaga aactgtttct ccattgatca acccagcgcc taacacaacg
    19441 atgaagtctt gattgtatcg aggccggttg aactgataca agaatgagac agtcaaaaag
    19501 ttgtagaata cgataaatag atagatcaag atcaggggaa cgcagaaaaa caaagcattt
    19561 atccattgcg gcaaatattt taacaagaaa aagtcaaaaa caagaaacag tgtaagaaaa
    19621 atggctagta tcaacgttaa aagattagct aatgaatgcg attctctacg taaaactgtg
    19681 accccgttcc aaaaaagaaa aacaatcaat ccataaatac caaataaaag gacaagtagc
    19741 aatggaatcg tgatcaaggc gatcagtccg ccaaggaaaa tattttgcgt ttcatacagc
    19801 aagacaaaca agtagatgcc gaaactgatg aggaaaacat tgaataaaag accattcagc
    19861 agccttcgtt tttctgtgaa ataagaaaaa gcaaagatac taaagaacaa caatggaatc
    19921 aaaaaataaa aggtcgaata aaaaatttcc aacacgacaa aataagttaa gattaccgta
    19981 aatgtccacg tactgccgta aaagaacaag gaaggatcct tcttctgttt tcgccaataa
    20041 acgatagaga ataccccaat aagatagaga actaaaaaga tcagatctct ggttagattc
    20101 ataaattcaa ctcctttttc ttatacagta taacgaaaat agatgaaaaa agctgttatt
    20161 taaactagaa aaagcaaaaa aacatctttc cgatggaaag atgtttttct tattgttcaa
    20221 ttaaaacaag ttgccgaaaa attctgcgac gcgtcgccag ccaatcacga atatattcgc
    20281 tttttccact gttttatccg taatgatctc tgttgaaggt gcattgttgt cttctaaata
    20341 gcctaattta tcgtctgcca aggtgatcgt agcggtaccg atttttgtac ctttatccat
    20401 aggtgcttgg atctcattgt cagtgacttg cttcttgtca agtgttggcg tgatcgttaa
    20461 tttgccagta tccatatcgt ttcgtaccca tacttttaca gggcttttaa gcacaacagg
    20521 aactgttgat tcttttccgt cttttacatc aatggttttt agactaggaa tacttgcgtt
    20581 agcttttcct agctcttttt ctgaccaatt gtcatagcag tagtccatca aacggtctgt
    20641 ttcattgaag cgggcactcg ggttttccgc atggccgtcc gcattcaata cgacagtgat
    20701 gatccgttga tcgtttttag tgatcgtacc aacgaaacaa gcacctgcaa gctcagtcgt
    20761 accagttttt aatccgtcca cgccttcctt gtaattaacg aatcctggga gcatccagtt
    20821 ccagttgacc atttccactg gtgattgcgt attttcgcca aacatttggg tcgtagtact
    20881 tgatacatcc agtatttctg ggaaatccaa gatcaaatgg cgggcaacga tggccacatc
    20941 ttgagcagac atttgatttt cgtcgttttc tccagtgcct tctggtcggt tttcgccaag
    21001 ataagagttg ttcaatcctg atgcattaac gattgttgca tctttgattc cccaatcttt
    21061 caattgttct ttcattcgtt cagtgaattt agcttcgctg ccagagattt tttctgccaa
    21121 ggccaccatt gaagcattgg ctgactggat gattgcagag tcgaataatt ctttgacagt
    21181 gtaggtgttt tctttatgca aaggaacatt cgataaatct ggggtgatac tcaaattttc
    21241 tgcataatct gaaatcgaaa ctttatcatc ccatgacagt tttccttctt ttacttgatc
    21301 aaggacgatg taaagaccga tgatttttgt gattgaagcg atccccatgg ttttttctcc
    21361 atcttgatca taaagaatct taccgctttg cgcatctaca gcaaaagctg cttttgcatt
    21421 tactttgaac gtatcttcag cttgcacagc aacagctggt agaaaagaag caaggcaaag
    21481 aatgagacta gccaataata ggactagacg tgtttttttt atggacatat ccgatctcct
    21541 tgtaattaat ttgtttcgct acaaattaaa attttatcag agaggggaga gagtttcaat
    21601 gtttctgaaa aaactttagg ataagaacgc gcgttatgga agtttagcgg tcgtttttac
    21661 gtacttttac aggaataggc tgtgtttccg catctaataa gcgggaagcg ctgaaagcaa
    21721 ataaactgat aatacttaat aaactgaaaa tcataatcaa atgcataaga tccacctctt
    21781 ttattctttt tcttagttta tcagcatttc atgaagaata gttgaagact ggggctaaat
    21841 aattgaggaa attatgaaga taacttaaaa aaatagctaa atttaaaacc gtagaaaccg
    21901 ttttattttt ttagatacgt tttattgtat ggaaaaatag tgttgtggta gactttagat
    21961 agaaacaaag gaatgatttt tttaggaggg gtacgtttat ggcaaaaaag gataaagaat
    22021 tattgacaca attgacttca gaaggcgcta aaggaccatt cgtcacgatc atgctgaata
    22081 cgcatgtcgc ccatcaagat gtggaaaaag atcagatcaa attcaaaaat tttgcgaaag
    22141 aagcaaagaa acgttttgag aaaaaatatt ccgaacatga ctggtccccg ttccaagaaa
    22201 aaattgaaaa tttgttagga gatccttcgt tttggcgtag tgcaactacg agtgtagcgg
    22261 tcattataac tgctgaccag acctatatcc atcgtttgag tatcccggta gacgatcaat
    22321 actatgtaag tgatcttcct tatttactgg ctattatcaa aaatgggcag ttcaactatt
    22381 cttattattt attgggattg aaccgcgact ccatgaaatt gtatgaggtc agcaacaaac
    22441 aagtaaaaga agtatctcta ccagaagggg caccggttga tgtcgttact gcattgggat
    22501 ctgaactgac aggtggcggt ttgaacttct cttctaatgg aggaggcgga gcccgagatg
    22561 gagtaacttt ccatggcgta aatccaaaag atgaagaagt agagatcgat tgggtcaact
    22621 attaccaagc tgttgatacg tacttgaagg attctttcga aaatcctgaa caatggccga
    22681 tttatttatt tgcattacca gaaaatcaga cgatgttcaa aaaaattgct aaaaatccct
    22741 attattgtgc agacgttgcg atttctgctt ctcctgcgca gctatccttt agagatatcg
    22801 aagaaggggc gaagaagctg tcaagtgaac tcgcagctca agaatcaaaa gaatacaata
    22861 aactcttaga taagaaattc atcgatcagt tagttgatat cgttccagct gcgaaagaag
    22921 gaaaagtgtc acatttattt atcgctacgt caaacttagc tgatggtttc ggtgaagatc
    22981 ctgaaacaga atatgatcgg cgtcaagtgt tgaatgcatt agcagatgat gtgatcatta
    23041 atggcgggaa agtgtttatt ttggatcaaa aagatgcgcc agatgaaaag agtctgacag
    23101 cgattcttcg ttattaaaag aggttgtgaa atcagtgttt tgacctgagt attagagaaa
    23161 caaacggaaa aatagtttca catatttttc cgttttttga gctaataccg cagatgcaag
    23221 cttttgaaca ccgtttaagg aagaggttgt gacagaagtg gtcagcttca agaaataaga
    23281 agagattgac aaaaattatt tctcaaattt ttgtgaaatt tcgacttatt tccgaagaag
    23341 ctacttctgg aacaccgttt aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg
    23401 attatgatac aaaaaacatc atttatgaac aaaatgtgaa taacgggcgt tcttgagata
    23461 tgattttaat tgggaaaaac ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg
    23521 gtgtttgctg tttctatccc taagtttaac aagtcagcca gtttttgctg aagaaacaca
    23581 gcggtctcct attacaggca cacgtctgta caaaaatgtt tatgtacaga caacagatct
    23641 tgattctgcg attcctgaaa cagaagaacg gtctttgcag ccaagatttg gttacttttg
    23701 gtactgtccg acctgtgatt atgaaagttg ctggcatttc ttacattcta cagctgcagc
    23761 ttttgctttt actcatactc agaaatatga tggacatcat cctgttattt atgaggtata
    23821 gatatgagca aaaaagaaaa actaatctcg ttgatcgtat tggtttgcct tgttcttctg
    23881 gtaactgtca taggctatca gaaataccat gagaacatca gacctactaa agaatcttcc
    23941 ccagcctcga ccgttgaaac agaaccgata agtagtcaga aagaataatt tttttagttt
    24001 aaagaagaac atcagagcaa aaaaacacca actgaacaat atgtcgttta ttttaaaaaa
    24061 agttcggttt ttcactttat gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa
    24121 aaactctagc ttttttgtaa acggtatgat atactatagg agtagttttt gttcggtaat
    24181 ctgtggtagc ttgtttcaaa taaacatctt ccagatatac accattcaag taattgcaaa
    24241 atatgtgttg atacacaagg aggattttta ttatggaaac aggtacagta aaatggttta
    24301 actcagaaaa aggatttgga ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt
    24361 tctcagctat ccaaggcgac ggattcaaaa ctttagaaga aggccaagca gtaactttcg
    24421 atgttgaaga aggccaacgc ggacctcaag caacaaacgt tactaaagct taattaacct
    24481 atactaaaaa ataaacgccc agacatttgc gtgtctgggc gtttttgtct attttcttta
    24541 aaaagcgatg agagtatttt tctgcacttt gtagtataat ttcaaaagaa cattagaggc
    24601 aggagaagag gaagatattt tgaaaccaag aacttatgca gtcgttgata tcgaaacgac
    24661 tgggacgaat ccaaaagaag atcggatcat ccaatttggc tgcgtcatga ttgaatcagg
    24721 tcggatcact tcacgttttt cgatcgatat tcatccaggg cggaagattt caaaacaaat
    24781 ccagcattta acagggatca cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt
    24841 cgcccaaacg atttacaatc ttttggcaga caccatattt gtcgcgcata atatttattt
    24901 tgattataat tttttgaacc atgaactcat gcgatgcgga cttcctagtt tgaagatacc
    24961 aggcatcgat acagtagaac ttgcacaaat tttcttgccg acggaaccaa gctttcgctt
    25021 agctgatttg tcagaaagcc ttggatttat tcatgagaat cctcatcagg cagatagtga
    25081 tgccgaagtg acgggtcaac ttcttttgct gatcgaagaa cggatgagaa aattgccaat
    25141 catcacgtta gagcagattg cgcgattaag tagacacaca ggtatggata cgagtcgttt
    25201 catttatcat gtgattgaag aaatgaaaga aaaaccagag ccacttgatc cctcattaga
    25261 aatcgtcgat gggctagctt tgcagaaaaa agaagtggaa ctattcatat acgtccatta
    25321 tggcgagcgg acatatccaa gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact
    25381 gatgtatcga aaagaacaga acagactgat gaatgcggtc tacgaccatt tcacaaaaga
    25441 tgaatcaaaa gatttgatga tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct
    25501 gctgccattg agttatttgg ctacacctga aaaacccgct gtcatcagta ccgtttcgct
    25561 agttttgcag cagcagatca tagaaaaaga cattccattg ctgaatcaat tgctagatca
    25621 gcccatacaa cctgtaatta tcaaaagcta tcgccactat attgatttac agcgttttaa
    25681 aggcacactt gtggaaccgc cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt
    25741 agtttggctg acgcaaacga agaccggtga tttggatgaa ttacatttaa caaatttgaa
    25801 ccattcattc tttgcagata tcgcgcacag aggcactgga tttttagcaa gaaatcagtc
    25861 attttacgaa caagactttg ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt
    25921 gattgtcaat catgcgtttt taatacaaga aacacagcga aaagaaccat tgcttccagc
    25981 tagtccattt ttgttgatcg atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa
    26041 tgacaaacga ttaagtatta actatttcca taaacaaatc cagcaattag aagaaccaaa
    26101 tcagctgttt gaccgaatac aaagctattt accgaaagaa catgaagcct tacgctggtt
    26161 atcgatttat cgagaagaac tttctgcgct atgcgaagca cagatttgta tggcagaagg
    26221 attaatgagt ttagtgagcg gcaagcaacc taacggacag tatccagaag aagtcatgac
    26281 gacaaaagag atgttggatc agctgccact ggaatctggg acagccattg atcacctgca
    26341 gcttttttat caggagatcc agcagctaca gtcacaaatg cgtctgactt ttgagattat
    26401 ccaagaaagc tggacaaacc agatgcggat cgatttagca agtttatata gcttatttga
    26461 tcaattagac aagcagtact tgctgatgga tcaatggctg gaagactggc gagatcattt
    26521 gatccattgg ttcgttcctg ggaacacagc gcagcagatg atttttcatc tccatgattt
    26581 tcaagcggct atgttgccgt cgactgtttg gtatcctcga tatgatcgta ttctttatat
    26641 tggaggaacg ctgaagatcg gccctaatcg acactatctc tctaagcggc ttggcattga
    26701 agcagcacct cttaaagtga ttgcctctcc ttatgattat gagaaacagg caaggatcta
    26761 tgtgccaaaa gaggcacctg ccgtccatca agtcagcaat caagcatata tcgattattt
    26821 agagacagca cttacaggtc tgatcgaaca agaagatcgg ccaatacttg tgttattcac
    26881 ttctcatgat gttttgcaga aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg
    26941 acgagaaata ctagctcaag ggtttggcgg aagccgggag aagcttttga aacgcttcat
    27001 tttgtcagaa aaaagcgtat tgtttggcgc tgacagtttt tgggaaggtg tagatttgcc
    27061 tggagatgct ttgcagatcg ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc
    27121 gatggtcaaa gctaggaacg cttatttaga atcagagggg atcaatcctt tttaccagga
    27181 atccgtacct aaagcagctt tgaagttacg ccaagcatta ggacgcttga taaggacgga
    27241 gacagatcgt ggcgtattgc ttattttgga tcgaagacta gttactgcaa aatatggtgc
    27301 acggatcatc catgcattac cgaaaaaagt accgatcaaa gagctagcta tggaggaaat
    27361 ctatcaggat atccatgaat tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct
    27421 tttttcataa ttaaaatctg ctataatgaa aaggaatgaa gataaaggag cgacgaccct
    27481 tgaagaaaaa acagacaaag gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat
    27541 ttttattagc ggtcatcgtg atcagctcga ttttttactt gcgttccagt cgtccggcgg
    27601 tgcaggcgaa gaaagaagca gtagaaatcg caaagaaata tgcaaatctt gaggaagccg
    27661 atcattttta ttggtttact cgaaagaaga catacttttc actgacaggt acaaatgata
    27721 aaggaaaaga aatcgccgtt atcattccta aatctggtga aaaagtaaaa gtattggatc
    27781 aaacaaaggg actgactgaa aatgaagcaa aacagacaat tgctgcggcc catcctgaaa
    27841 tccaaatcga aaaagctgct ttagggatgt atgatgatca gcctgtgtgg gaagtagtag
    27901 gaaaagaaac aaatgggaaa ctgaattatt acctgattgg ctttgaatct ggagatgaag
    27961 tcaagacaat caagggaatt taaaaggtaa aggaatgaat gggatggagt tatcaaaaag
    28021 aacaaaacga ttagaaccgt ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa
    28081 agccaaaggc agagatgtgc tgagtttgac tgtgggagaa ccggattttg cgacaccaga
    28141 aaacatccaa gaagcagcaa tcgaagcaat ccgcaatggg aaagctagct attatacacc
    28201 aacagcagga atcccagagc ttcgacaggc aatcgttgat tatctgaaga aaaactatgg
    28261 acttgtctat gaaccttcac aaacagtcgt aacagacgga gcgaaatttg ctttgtatac
    28321 ccttttccaa acgatccttg acccgcaaga tgaagtcatc attcctgtgc cttactgggt
    28381 cagttacggt gagcaagtaa aactggcaga aggacttcct gtctttttga aaggagaaga
    28441 atccaatagc tggaaagtta ctgttgagca gctagaacaa gcacgttcag aaaaaacaaa
    28501 agccgtaatc atcaattctc catcgaatcc gactggaatg atttatagca agaatgaact
    28561 gcaggcaatc ggtgaatggg cagtaaaaaa tgatattttg atagttgctg atgatattta
    28621 tggccgttta gtttataatg gcaatgagtt tactccaatt gcaacgattt cagaagcaat
    28681 caaaaatcag accatcatca tcaacggcgt gtctaaaaca tatgcgatga caggctggcg
    28741 cattggttat gcggtgggaa atcctgtgat catcaatgga atgatcgctg ttgcttccca
    28801 atcaactagc aatccgacag cagtcagtca atatgcagct gttgaagcat tgaaaggcga
    28861 gcaagataca gtggaagaaa tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc
    28921 gcttgtggca gaactgccgg gtgtttctct tgaaaagcca caaggagctt tttatctttt
    28981 tcctaatgta aaagaaacgc tacgcatgtg taaatatgaa aatgtcacac aatgggtaga
    29041 agatctttta gaagaaacag gggtagcgct tgtgactggc gaaggctttg gggcaccaga
    29101 aaatgtccgc atgagttatg ccactgatcg cgctacgtta gaagaagcgg tacgcagaat
    29161 caagcaattt attgagagta aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa
    29221 aggagagtca tacgtggaac aaattcaaat catcgattcg aaaaaccatg tgggagaaac
    29281 agtaacaatc ggcgcttggg tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca
    29341 attaagagac ggaacagcct ttttccaagg tgtagttgta aaaagcgaag tgccggaaga
    29401 agttttccaa acagctaaaa atctgcacca agaaacatcc gtgttagtaa ctggagaaat
    29461 ccgtgaagac agccgttcaa aatttggtta tgaaatcgga attacaaatg tggaagtggt
    29521 aagcgagagc cacgattatc cgatcacacc aaaagaacac ggaacagact tcttgatgga
    29581 tcatcgtcat ttatggctac gttcttcaag acaacatgcg attatgcaga tcagaaatga
    29641 aatcattcgt gcaacgtatg aattctttaa tcaacatcat tttgtcaaaa tcgatccgcc
    29701 gatcttgact ggttccgcac cagaaggcac tacggatcta ttcgaaacaa attactttga
    29761 tcaaaaagct tatttatctc aaagtggcca attatacatg gaagctgctg ctatggcttt
    29821 cggaaaagtc ttttcattcg gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca
    29881 tttgattgaa ttctggatga tcgaaccaga aatggctttc actcatcaag aagaaagttt
    29941 agaagtacag gaacaatatg tagcattctt agtacaaaat gttttagatc attgcgaata
    30001 cgcattagat gtactaggac gagacaaaga agtcttaaaa acttatacaa aattgccatt
    30061 ccctcgtatt acttatgatg atgcgatcga attactgaag aaaaatggat ttgatgacat
    30121 cgaatggggc gaagactttg gttcacctca tgaaacgttc attgcgaatt cattcgaaca
    30181 gcctgtcttt atcctaaact atccaaaagc tatcaagcca ttctacatga aaccacatcc
    30241 aacaagagaa gacgtggtaa tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat
    30301 tattggcgga agcgaacggg caacggacta tgatttcttg ctagaacaaa tccgtaatca
    30361 cggtttggat gaaaaagaat attcatggta tcttgatcta cgtaaatacg gttctgttcc
    30421 gcactctggt tttggtctag gccttgaacg aacagtcact tggattgctg gtatcgacca
    30481 tgtgcgtgaa gctattccat tcccacgttt gttgaaccga atttatccat aagaggtgat
    30541 gaaaaagctg ttctgcatca agtaattaga acagccaagc aaaaacagct gttcatgttt
    30601 ttgaattggc gggacttatt accgcagatg taagcttttg aataccgttt agtggagagg
    30661 ttgtgaaaaa gctgtttagt ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac
    30721 atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg tttaaggaag
    30781 aggttgtgaa atcagtgcct tgacctgagt attagagaaa caaaacggaa aaatagcttc
    30841 tcatatttta ccgttttttt agctaatatc gagggtcagc tgattgaaca ccgtttatat
    30901 caagaggttg tgcgcctgcc gttcagctcc gagtcacaag gagtaatttc aaaaaacagt
    30961 ttttcatgtt tgatgactat tgtgacttgt gaccgaggag ttggccggag agcaccgttt
    31021 attctaaaaa agaactgcga actctcgcag ttcttttttt aaaatacggg ataaaggaag
    31081 tttccagcag acaagagaag aaagcaaaag aaattgctat ttgattcaca aggttctata
    31141 acgaaatcgc aatcaatcaa acatttacaa agaaggtaat tcaaatggaa atctgatttg
    31201 atgatgcttc tgctgttgaa ataaagcagc atctagcacc tgaaaacaaa ctaatagtaa
    31261 cattcgaaga tggagtaggt ccgtattttc agcatgcaat aatccatatg taagttcaat
    31321 tcacccttga aatcatcgga ccagataaag ttatgaccaa tctatcccaa gcaatctaga
    31381 agagatatta gtaaagggac atttactaga agtcttagat gagcctataa atatatgttc
    31441 tgatggcgtc atcagtggtc tgattgacgg aagcttaggt gtgatcgatc tctctggacc
    31501 aaacaacgaa ggaatcaagg ggaatccggc acgttaagaa aaaaatagca taaaaaacta
    31561 gacacttgat gagcgttcat tcaattgttt gaagggacta ggctggtgtc attttttata
    31621 catatatttt ctctttcgtc ttgatagggc ttatgggata atataaggaa gaaaatcatc
    31681 gttttattca aaaaggagtt tttgacaaat gaaaaaacgt atattattta tcggtgatag
    31741 cattacagac gcaggaagag accgagcaga agcaactgat ctaggtaaag gttatccact
    31801 tttgattgcc ggaaaactaa tctcccaata tccaacgatg gaatttgaat gcatgaaccg
    31861 tgggatagga ggagacaaaa ttgcagatct gcaagtacgc tggcaagaag attgtttagc
    31921 tttacaacct gactatgtat cgattttgat cggaatcaat gatacctggc ataatactag
    31981 cgatcaggct atattcggaa ctgagaaatc tgctcagcaa tttgaatccg tttatcgaaa
    32041 attactaaat gaacttcaaa cagtgggaat ctgcaacatc atcttgatgg agccgtttgt
    32101 acttcctata accgacgacc gtcatggctg gcgtaaagac ttagacccta aaatccaaat
    32161 tgttcgtaaa cttgctaagg aatatggcgc attgctggtt cctttagatg gtatattcaa
    32221 tgctttaggg attgtttatg gtttttcgaa atatacaaga gaagatgggg tccacccaac
    32281 gattggcgga catgagatca ttgcacaaag ctggatagat gcaattgccg cagtcgattt
    32341 ttttagtgga gataaaaatg agcgaaagaa aagtgagaaa tgatggaaat acggtgggca
    32401 gtaaaagaag atctacctca aatcaatcag ctgattactg atcatccaga atcattcgta
    32461 aaagcaaaaa cggcagctga cctaaatgat aagaataaac tattgctcgt tgctgtaaca
    32521 aaagaaaaac ggattatcgg atttgcgcaa gcggacgtgc aacagtcaga agaacgattg
    32581 gatcagcgac catcaggaaa actaatccaa cacacattag taacaaaaga aaatacggaa
    32641 gaaatccaaa aaaaatttgc agaatatttg atactggaag ctaaaaaaag aagaataaaa
    32701 tcattttttg cccagtttta atcaaaaaca gtaatagaag gagaaataca atgagttgtt
    32761 taggaaatgt catctggttt atattcggcg gactaatcgg tggtcttagc tggttagcag
    32821 cggggatact ttggtgtatc acgatcatag gtatcccaat cggtatgcaa tgttttaaga
    32881 tcgcctccct ttcttttgcg ccttttggca aagaagtaat ttatcaccag tctggcatgt
    32941 cattgattgt taatatcatc tggctgatcg tttcaggcct accaattgct ctcgcacatg
    33001 tcgtcagcgg catccttcta tgtatcacga tcatagggat accttttgcc aagcaatctt
    33061 tcaaacttgc tcgattggcg ttgatgccat tcggtgcaga aattgtataa aagagaataa
    33121 taaaggttag ataaagcata gagtcaggtg ggatacatgt atttcacctg actctatgcg
    33181 ttctcctgta taagttcttc catgtgactt ttacgtcttt tatttatatg gggttactgt
    33241 ttatggaaag ttgaaataat tttcattaaa gaactctttc tatatttttg aaagcgtagt
    33301 ggagcttcga atatctggga aggacatttt gcagggattg aaaaaagata ttgtcctatc
    33361 gaaaaattaa tgaatttaga caaattgcca gcaacaggct tcaaacttag ttgctatcct
    33421 gtcaagatca agaaggcaag tgcgggttgg ataagagcag tggcaatgat cgaaaagaaa
    33481 aagaaagaat aagccaaaac atctgtatgg aagaaatttt ccgatgcaga tgtttttcat
    33541 tttttctttc ggataccttg acatatgaat aaccgttcat ttataattag ttatgaagag
    33601 atattcatat gttgaggcga ggaggtcatt aaaatgaaaa gttacagatt agaaggttta
    33661 gattgtgcga actgtgcaat gaaaatcgaa aaaggagttc agaagatcaa tggagtcaag
    33721 gaagcaacgg taaacttcac ttctggaaaa ctaacgattg acgcagagga agatcatttg
    33781 gctacgattg aacaagaaat aaaaaaagta gtgaaggaat tagaaccaga tgtcaaagta
    33841 acagaaatag ataaggaaaa agtatctgaa catggaaatg aaaaagagag aaatacgctg
    33901 ttccggattt tattttccct tgcaggtatc gctttattgc tattatttga ttttaatgaa
    33961 ccaattcgct tgatagggta tttattgatt tatctgctga taggatatga tgtagtgaag
    34021 aaggcagtga tgaatatcgt caaaggaaaa atctttgatg agaattttct tatgtctgtt
    34081 gctacgattg gcgctatgtt gatcggtgaa tatccagaag ccgtagcagt gatgctattt
    34141 tatcaaatcg gcgagtattt ccaaggattg gcagtcagtc attcaagaaa atcgattcgc
    34201 gaattgatgg cgatccgtcc agaaacagct cacgtgcaga cgacggaagg attgatgacc
    34261 gtgaaccctg aagacgtgca gatcggtcag tttgtacttg tgaagcctgg tgaacgtgta
    34321 ccgttagatg gaacgatcat cgaaggagaa tcattagtcg atacgtcagc acttacagga
    34381 gaatcagtcc ctaaatctgt atatgttggt gaaacagtat taagtggatt tatcaataaa
    34441 aataaaccat ttttagttca agtagaaaaa tcttatgaaa attctactat cagcaaatta
    34501 ttagaattag tagaaaatgc cagcagtaaa aaagcaccag ctgaaaattt cattactaaa
    34561 ttcgcgcgct attacacacc agtcgttgta ggattggccg ttttattagc tgttttacct
    34621 ccacttgttg tttctggagc ggcgttttct gaatggatct atcgagcatt gacattttta
    34681 gtgatctcat gtccatgtgc attagtgatc tccgtacccc ttagtttctt tggcggtatc
    34741 ggaggagcaa gtaaaatcgg tgtattagta aaaggttcaa attacttgga gttgcttgca
    34801 caaactgaga cagtggtatt cgataaaacc ggcacattaa cgaaaggtga cttttcgatc
    34861 cagacgatcg atacaaaaat cgatccaaag atattcatgc aatatgttgc gagtgcagaa
    34921 cagttttcta cacatccgat tgctcaatct gttttagaag gctacgcagg tccgctttta
    34981 ccaacagcta atatccaaga atttgcaggt gaagggatct tagctgaagt tgatggcaaa
    35041 caagtattag caggtaatca caaattgatg gaaagatttg aaatctcttt tccttcaagt
    35101 caagaaatcg gtacattgct ttatctagcg atcgatcaaa gttacagtgg ttatcttgtt
    35161 attgcagata ccttgaaaga agatgccgta gacgcattgg ttcaattaaa aaaggctggt
    35221 gtgaagaaca cagtcatgtt gacgggcgat tcgaaaaaaa ttgctgatca tatcgggaag
    35281 caagtaggcg tggacaagat atatagcgaa ttgcttccag aagataaagt gcagcggtta
    35341 gaggaaatct tacaaagaaa caataaaaag accgcatttg tcggagatgg aatcaacgat
    35401 gcaccggtgc tagcaagagc agatgtagga atcgcaatgg gtggattggg aagcgatgct
    35461 gcaattgaag cagcagatgt agtcatcatg aatgatcaac catcgaagat tgcagaagca
    35521 atccatttag caaagaaaac attgaaaatc gtcaaacaga acattgtctt tgctattggg
    35581 ataaaaattt tggtacttgc cttaggtgct tttggttttg catcaatggg agatgcagta
    35641 tttgcagatg ttggggtgac agtattagct gttttgaatg ccatgcgaag cttgcatgta
    35701 aaaaataaac gttgatcaat gaagaggacg aatgaacgtc ctcttttttt tatatccttt
    35761 tcttaatgaa aaatgtaaag aaaatagata ggattataaa ataactaatg tttttaattt
    35821 tgcagaaaat tcaaaatagc ttgaaattca ataaggtaaa tgaaataaaa gaaaataact
    35881 tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat taaataaaag aatacacaca
    35941 taaaataaca ctgtcttgta agtttgttaa aaattgaata tgattacatc tggataacaa
    36001 atttttatca taaaggagag aacgaatgaa caacaaacaa ttacgctggt ttactgttgc
    36061 gttaattgca tttaacatgg tttggggcat ggggaatgta gttaataact atgctcagca
    36121 aggagtatca gttgtcacct cttggctatt gattttggcc atttatttta ttccatacgc
    36181 attgatcgtt ggacaattag ggtcagcctt caaggatagt aaaggaggcg tcagttcatg
    36241 ggtcgaaaat acgacatcta ataaacgact ggcatactat gccgcttgga cttactgggt
    36301 cgtccatatt ccttatttgg cacaaaagcc tcaagcaatc ctgattgctg gcggttgggc
    36361 agtccaagga aatggaaata tcgtaaatac gatgtcagta caaatggtag cagttatttc
    36421 acttattata tttcttgcct ttttatattt atcaacaaaa ggactttcta ctttgaaagt
    36481 gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg atcttattta ttcttttagc
    36541 tgtaacggca ccgttcatca atccaacgat ggaatttgcc acaccacata tgaatcaagt
    36601 gaaaacatat attccaacat ttgatttcag ttatttcaca accgtttcga tgctagtatt
    36661 tgcagttgga ggagcggaaa aaatatcccc ttatgtcaac tctacaagaa atcctgcaaa
    36721 ggaatttcct aaagggatga tcttcttagc agcaatggta gggatttgtg ctgtattagg
    36781 ctcagtcgca atgggtatgc tgtttgctag cgataatatt cctaaagact tgatggcaaa
    36841 tggggcatac agtgcttttc aaattttagg taattactat ggtatcggta actcattgat
    36901 gatcatttac gcattgacaa acatgattgg acaaatctcc gcattagcct tttcaattga
    36961 tgcaccacta aaaatcttgt tagcagatgc ggatccagaa tttgtaccaa actggctacg
    37021 taaacgtaat cgtaaaggta cattggtgaa tggttatctt ctgacaggta tactagtagg
    37081 aatcattatt ttgttgccga tctttggtat caaagatatg aatgaattag taaaatggtt
    37141 gacgaacttg aactcagttg tcatgccaat gcgttaccta tgggtgttct tggcatatat
    37201 gatgttgaat aaagcttata agaaattcaa ttctgaatat aaatttgtta aaaatccaaa
    37261 aatcggtttt atctttggtg cttggtgctt tgccttcact gcgtttgcat gtatcctagg
    37321 aatggtgcca aaagtagatt atactgctga tcctaaagca tggtggttcc aattattcac
    37381 gaatatcatc acaccaattg tcttgatctt attaggtatg atcttgccag caattgctcg
    37441 tcgagacaaa aacaaagaaa tcactgaacc agaaacagtg gttcagcctt aaaatagaaa
    37501 tacttgttac aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaatttac
    37561 gaaaattgct tctcaaattt ttgtgaagtt tcggcttgtt tttcgcagaa gctacttctg
    37621 gaacaccgtg aatcagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
    37681 aacaaaaata gcccctcata tttttcgttt tgctgggctt attaccgcag atgcaagctt
    37741 ttgaacaccg tttaagaaag aggtagtgag cctgccgttt aactccgagt cacaaggagc
    37801 aatttcaaaa aacaacttct catgtttgtt gactattgtg ccttgtgacc gaggtgttgg
    37861 caggagaaca ccgtttaagg aagaggttgt aaaatcagcg atttgacctg agtattagag
    37921 gaacaaacga aaaaatagct cctcatattt tgccgttttt tgagttaatg ctgaaggtca
    37981 gctgattgaa caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc
    38041 ttgttttttt gcggcgttat aagtggtatg atcgaaccaa attccaaaga gaagtgaggg
    38101 agaagaatga agatcatcgt gattggacaa agtgggtcgg gaaaatcgac cctcgcaagg
    38161 aagatcaaag aaatcaccga ctttccgcta ctgccgctag accttttatg gcatacaaca
    38221 gactacagta ttcaggcaaa aaagtggttt ttgcaagaac agcaatcgtt tatggtatct
    38281 aatccgtcat ggattgttga aggaaattat acttctacat tagaggaaag gattaaagaa
    38341 gcagataaga tcatttggct taaagtgccg cgttatctcg ccatctaccg agtagtttgg
    38401 cgttctctaa aaagaaagat aaacaaaaaa tctcggccag atatgcctga atcattttca
    38461 gagaaactaa acagggaata cttagaattt ttatctttta tctggcattt tgaagaaaac
    38521 aatgaaccga aaatacagca gctgatccaa gtagctaatg caagaaataa actgatcatc
    38581 cttaaaacaa gacgagagaa acaagaattt ttaactaaat tgaaaataga gagcaaaaat
    38641 aattgacagt ttttgtttac aagcgtaatc tgtattcata agctaatgat tacatttgta
    38701 aacgaaaggt gtgaaataaa tggaaaaagt caaaacgcca gtaaagatca gtgattcaga
    38761 gtgggagatc atgcgtgtta tttggacact tggacagacg acagcccaag aaatcacgca
    38821 gatcttagct gattcgatgg attggaaacc agcaacgatc aaaactttgc tgggacgttt
    38881 ggtaaaaaaa gaagtgatct ggacagaaca agaaggaaaa aaattcattt atcatccagc
    38941 tgtctcggag atggaaacag ttcgcagtgc gacggaaaat cttttttccc atatctgtgc
    39001 aaaaaggatt ggagagacga ttgcggatct agtagaagaa gccactttga ctcaagaaga
    39061 tatcaatgag attttgaaac aactggaaca aaagcaacca gtagatacga tcgaatgtaa
    39121 ctgtatccca ggtcaatgtg aatgtaaaca acatcaatag gaggaagaaa aaatgaaaca
    39181 acaattttca atcgaaggta tgagctgcaa tcattgtgca gcaagagtag aagaaacagt
    39241 aagcgcactt gacggcgtgc aaaaagtgaa agtaaatttg aaaaaagcaa acggtacggt
    39301 caagtttgat gaaacaaaag ttcaatccga aaagatttgt caagcaatca atggattggg
    39361 ttataaagca gaggtgattt gatggcaaca gcagaaacca aaatggatac gttcgtcatt
    39421 acaggtatga cttgtgcaaa ttgttctgct aggatcgaaa aagagctgaa cggacaacct
    39481 ggtgtggtca atgccacagt caacttggcc actgaaaaag caagtgtcaa atatgaaggg
    39541 acgaccactg aaaaattaat ccaaagcgta gaaaacatcg gttatggtgc aattttgtac
    39601 gatgaagccc acaaacaaaa aattgcagaa gaaaagcaag cgtatttgaa aaaaatgcta
    39661 ttcgatctta tattgagtac ggttttgact ttgccgttga tgttgtcaat gattgctatg
    39721 atgttaggaa gccatgcggc gattgttcac ttttttcatt ttccaatcgt ccagttagtg
    39781 ctgtctgctc ctgtccaatt ttatgtcggt gctcgttttt ataaaggtgc gtaccatgca
    39841 atcaagacga aagcaccaaa tatggatgta ttggtagcaa ttggaacatc ggctgccttt
    39901 gcattgagca tttacaacgg atttttccgt ggacaccccc aagatcttta ttttgaaagc
    39961 agcagtatga tcatcacgct gattttgctc ggcaaatatt tggaacatac agcaaaaacg
    40021 aaaacaggta atgcaatcaa acaattgatg tctcttcaaa caaaaaccgc ccaagtgata
    40081 agaaacggga aagaagaaac gttagcgatt gaagaagtag tcgttggtga tcaactagtc
    40141 atccgtcctg gtgagcagat tccagcagat ggtcgaatta tttcaggaag cagtgcgata
    40201 gatgaaagta tgcttactgg tgaaaatctt cctgtagaaa agaatccgga tgacacgctt
    40261 tttggcggga caatcaatac gaatggtctg ctacacatgg aagtcacaca agtcggcaag
    40321 cagacagtat tagcgcaaat cattcaaatg gtggaagatg ctcaagggag caaagcccca
    40381 atccaaaaaa tcgcagatcg gatctcaggg atttttgttc cgatcgtatt agtcatcgcg
    40441 tttatcacgc tcatagctac aggtttgatc actggtgatt ggcaactcgc attgatccat
    40501 agtgtctctg tattagtcat tgcgtgtcca tgtgcattag gtttagcgac cccaacagct
    40561 attatggtag gaacgggtgt tggtgcacga aatggaattt taattaaagg cggagaagca
    40621 ttagaagcag ctgctcactt agacagcatt gttttggata aaactggaac gattacagaa
    40681 ggaaagccca aagttacaga tctagtcggt tcaaaagaag tcctatctat tttttatact
    40741 ttagaacaag cttctgaaca tccactaggt aaagcaatcg tggaatacgg aaaactgcaa
    40801 gaagcagcaa catacgatat gattgatttt acagcccacc caggtgctgg gatcagcggt
    40861 acgattaatg gagtacgcta ttttgcgggt acacgtaaac gattaattga attgaaccta
    40921 tcatttgatg aataccaaga acacgcgtta gaattagaac agcagggaaa aacagtcatg
    40981 tttttagctg atgaaaagca agtgatcggg tggattgccg tggctgatca aatcaaacta
    41041 gaagccaaac aagcaatcaa gcagctccag aataaaggat tagatgtttt tatgctgaca
    41101 ggagacaaca aactggcagc cgaaacgatt ggtaaacaag ttggtattga tccaaagcat
    41161 atctttgctg aagtacttcc agaggataaa gcagcctacg tagaaaaact tcagaaagat
    41221 ggcaaaaaag tcggtatggc aggcgatgga atcaatgatg caccggcctt agcattagct
    41281 gatgtcggta tggcaatggg aagtggaacg gacattgcga tggaaacagc cgatgtgacg
    41341 ctgatgaata gcagtttggc atccattgca caaacaatcg agttgtctag gttgacattg
    41401 agaaaaatca aacaaaatct tttctgggct tttgtctata atacgattgg tattcctttt
    41461 gccgcactag gctttttgaa tccaatcatt gcaggaggag cgatggcgtt tagttccgtc
    41521 agtgtattgt tgaactccct gagcttaaat cgacacatga aaaagtaaga gaagggatgg
    41581 agtaaaaatg aatgggaacg atatggagaa aaaacatgag cataaaaaag aagtagtaaa
    41641 caaagaaagt acagaatctc atgaaaaaca gatggagatg aaacatgacc atagtcagat
    41701 ggatcattcc atgcacatgg gacataacca tggagatata gcaaacagca aacaaatgga
    41761 ccattcgatg cacatggatc acgaacacgg cggcatggat cattccatgc acatggggaa
    41821 tttcaaacaa aaattttggc tatcgcttat cctagctatc ccaatcattc ttttttcacc
    41881 aatgatggga atggaatttc catttcaagt aacgtttcct ggttctgatt ggctcgtatt
    41941 gatcttagca actatactat ttatctatgg cggtcagcca tttttaagtg gagctaagat
    42001 ggaattgaaa caaaaaagtc ctgctatgat gacactgatc gcgatgggga ttacagttgc
    42061 atatatttat agtgtctatt cgtttatcgc aaatctgctc aatccacata cacacgtcat
    42121 ggatttcttc tgggaattgg ctactttgat cgttattatg ctactaggtc actggattga
    42181 aatgaatgct gtgtcgaatg ccagcaatgc tttacaaaaa ctagcagaat tactgccaga
    42241 atcagtgaaa cgcttgacga aagatggaaa agaagagaca gtttcattga aagaagtaaa
    42301 cgaaggtgat cgtttgatcg tccggtctgg tgataaaatg ccaacagatg gtgtaatctt
    42361 gaaaggtgaa acaattgttg acgaatcagc tgtgactggc gaatcaagag gaatcaaaaa
    42421 acaagccaat gacaaagtga ttggcggttc tatcaatggg gatggaacga tcgaaatcga
    42481 agtaactgga actggtgaaa atggttatct agcgaaggtt atggaaatgg tgcgaaaagc
    42541 acaaggagaa aaatccaagc tagagtcatt atctgacaaa gtggcaaaat ggctgtttta
    42601 tgtcgcatta attgtgggta tcctggcatt catcgcctgg ttgttcttga cagatcttcc
    42661 taatgcatta gaacgtatgg taacagtctt tatcatcgcc tgtcctcatg ctctaggttt
    42721 agctatccca ttagttgttg ctcgctccac atcaatagca gctaaaaacg gattactttt
    42781 gaaaaaccgc aatgcactgg agcaagccaa tgatctagat gtgatcatgc tggacaaaac
    42841 aggaacacta acagaaggaa aatttactgt aacgggtgtc gaagtacttg atgatgcgtt
    42901 caataaaaat gaaatccttc aatatctagg agccttggaa gcgaatgcca atcatccatt
    42961 ggctgttggc attatgaatt atttgaaaga acatgaaatc aagccttatc aagctgaaaa
    43021 tctaaaaaat ctttcaggag tcgggctaga ggcaacagtc aaaaaacaac aagtgaaaat
    43081 agttaatgaa aaagaagttg agcgtctcgg attaaacgta gaacaagcat tattgaaacc
    43141 ttaccaagaa caaggaaata ccatcagctt cctgatttta gaaaatcatc tagctgcaat
    43201 cgttgcttta ggtgacgtag taaaaacaga agcaaaagaa tttatccgaa cattgaaaga
    43261 aagaaaaatc actcctgtca tgcttactgg tgacaataaa aacgctgccc aagcagtagc
    43321 agattattta gggatcgaag aatattatgg cggtttatta ccagatgata aagaagctgt
    43381 tgtccagaaa taccttgacc aagggaaaaa agtaatcatg gtgggtgatg ggatcaatga
    43441 tgcgccaagt ttagcccgtg cttcaatcgg gatggcaatc ggtgcaggga cagatatcgc
    43501 aatcgattct gcggatgtcg tattgacaaa cagcgatcca aaagatattt tgcattttct
    43561 tgatctagcc aagcaaacgc gaaagaaaat gatccagaac ctctggtggg gggcaggtta
    43621 caatattctt gcaattccgc tagcagccgg aattcttgcg ccaattggta tcatattaag
    43681 tccagcagtt ggtgctgtct tgatgtcgtt aagtacggta gttgtagctc taaatgcttt
    43741 gacattgaaa atcaaataat aatgaaaaaa g
//
LOCUS       DADXTH010000011        43609 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.011, whole genome shotgun sequence.
ACCESSION   DADXTH010000011 DADXTH010000000
VERSION     DADXTH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.011"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..544)
                     /locus_tag="IWI97_000598"
     CDS             complement(<1..544)
                     /locus_tag="IWI97_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBC2462128.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLK"
     gene            complement(621..1151)
                     /locus_tag="IWI97_000599"
     CDS             complement(621..1151)
                     /locus_tag="IWI97_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBC2462129.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1155..2015)
                     /gene="rlmB"
                     /locus_tag="IWI97_000600"
     CDS             complement(1155..2015)
                     /gene="rlmB"
                     /locus_tag="IWI97_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBC2462130.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2008..2403)
                     /locus_tag="IWI97_000601"
     CDS             complement(2008..2403)
                     /locus_tag="IWI97_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBC2462131.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2400..3812)
                     /locus_tag="IWI97_000602"
     CDS             complement(2400..3812)
                     /locus_tag="IWI97_000602"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBC2462132.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3809..4345)
                     /gene="cysE"
                     /locus_tag="IWI97_000603"
     CDS             complement(3809..4345)
                     /gene="cysE"
                     /locus_tag="IWI97_000603"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBC2462133.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4431..4670)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4667..6127)
                     /locus_tag="IWI97_000604"
     CDS             complement(4667..6127)
                     /locus_tag="IWI97_000604"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBC2462134.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6297..7454)
                     /locus_tag="IWI97_000605"
     CDS             complement(6297..7454)
                     /locus_tag="IWI97_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBC2462135.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7488..8858)
                     /gene="radA"
                     /locus_tag="IWI97_000606"
     CDS             complement(7488..8858)
                     /gene="radA"
                     /locus_tag="IWI97_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBC2462136.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8921..9448)
                     /locus_tag="IWI97_000607"
     CDS             complement(8921..9448)
                     /locus_tag="IWI97_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBC2462137.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9678..10484
                     /gene="proB"
                     /locus_tag="IWI97_000608"
     CDS             9678..10484
                     /gene="proB"
                     /locus_tag="IWI97_000608"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBC2462138.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10481..11728
                     /locus_tag="IWI97_000609"
     CDS             10481..11728
                     /locus_tag="IWI97_000609"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBC2462139.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11804..12148
                     /locus_tag="IWI97_000610"
     CDS             11804..12148
                     /locus_tag="IWI97_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462140.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12265..12591)
                     /locus_tag="IWI97_000611"
     CDS             complement(12265..12591)
                     /locus_tag="IWI97_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462141.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(12964..14165)
                     /locus_tag="IWI97_000612"
                     /pseudo
     CDS             complement(12964..14165)
                     /locus_tag="IWI97_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14282..14797
                     /locus_tag="IWI97_000613"
     CDS             14282..14797
                     /locus_tag="IWI97_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBC2462142.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(14996..15442)
                     /locus_tag="IWI97_000614"
     CDS             complement(14996..15442)
                     /locus_tag="IWI97_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBC2462143.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15489..16967)
                     /gene="msr(C)"
                     /locus_tag="IWI97_000615"
     CDS             complement(15489..16967)
                     /gene="msr(C)"
                     /locus_tag="IWI97_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBC2462144.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17321..17950)
                     /locus_tag="IWI97_000616"
     CDS             complement(17321..17950)
                     /locus_tag="IWI97_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBC2462145.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(17934..18398)
                     /locus_tag="IWI97_000617"
     CDS             complement(17934..18398)
                     /locus_tag="IWI97_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2462146.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(18885..20153)
                     /locus_tag="IWI97_000618"
     CDS             complement(18885..20153)
                     /locus_tag="IWI97_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBC2462147.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20384..21121)
                     /locus_tag="IWI97_000619"
     CDS             complement(20384..21121)
                     /locus_tag="IWI97_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2462148.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21188..21757)
                     /locus_tag="IWI97_000620"
     CDS             complement(21188..21757)
                     /locus_tag="IWI97_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBC2462149.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            21885..22757
                     /locus_tag="IWI97_000621"
     CDS             21885..22757
                     /locus_tag="IWI97_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2462150.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23099..23971
                     /locus_tag="IWI97_000622"
     CDS             23099..23971
                     /locus_tag="IWI97_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2462151.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            23968..25926
                     /locus_tag="IWI97_000623"
     CDS             23968..25926
                     /locus_tag="IWI97_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2462152.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26177..27028)
                     /locus_tag="IWI97_000624"
     CDS             complement(26177..27028)
                     /locus_tag="IWI97_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2462153.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27151..27618
                     /locus_tag="IWI97_000625"
     CDS             27151..27618
                     /locus_tag="IWI97_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBC2462154.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(27881..28363)
                     /locus_tag="IWI97_000626"
     CDS             complement(27881..28363)
                     /locus_tag="IWI97_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBC2462155.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28463..29326)
                     /locus_tag="IWI97_000627"
     CDS             complement(28463..29326)
                     /locus_tag="IWI97_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBC2462156.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            29924..30772
                     /locus_tag="IWI97_000628"
     CDS             29924..30772
                     /locus_tag="IWI97_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462157.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(30943..31284)
                     /locus_tag="IWI97_000629"
     CDS             complement(30943..31284)
                     /locus_tag="IWI97_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBC2462158.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            31370..32077
                     /locus_tag="IWI97_000630"
     CDS             31370..32077
                     /locus_tag="IWI97_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBC2462159.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            32064..32387
                     /locus_tag="IWI97_000631"
     CDS             32064..32387
                     /locus_tag="IWI97_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBC2462160.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(32476..33513)
                     /locus_tag="IWI97_000632"
     CDS             complement(32476..33513)
                     /locus_tag="IWI97_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBC2462161.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(33960..34943)
                     /locus_tag="IWI97_000633"
     CDS             complement(33960..34943)
                     /locus_tag="IWI97_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBC2462162.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(35359..36129)
                     /locus_tag="IWI97_000634"
     CDS             complement(35359..36129)
                     /locus_tag="IWI97_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462163.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(36122..37057)
                     /locus_tag="IWI97_000635"
     CDS             complement(36122..37057)
                     /locus_tag="IWI97_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462164.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(37651..39300)
                     /locus_tag="IWI97_000636"
     CDS             complement(37651..39300)
                     /locus_tag="IWI97_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBC2462165.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(39495..40391)
                     /locus_tag="IWI97_000637"
     CDS             complement(39495..40391)
                     /locus_tag="IWI97_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2462166.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(40384..41064)
                     /locus_tag="IWI97_000638"
     CDS             complement(40384..41064)
                     /locus_tag="IWI97_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2462167.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(41065..41847)
                     /locus_tag="IWI97_000639"
     CDS             complement(41065..41847)
                     /locus_tag="IWI97_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462168.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(41840..42757)
                     /locus_tag="IWI97_000640"
     CDS             complement(41840..42757)
                     /locus_tag="IWI97_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2462169.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(42760..43044)
                     /locus_tag="IWI97_000641"
     CDS             complement(42760..43044)
                     /locus_tag="IWI97_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462170.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT        12572 a         9293 c         7829 g        13915 t
ORIGIN      
        1 ttttgagttt tactctcccg taagcgctca ttacttgctt gtgatccatt gctaaacatt
       61 cagccatgtc tacaaaatca acgccttgca tataataatg aaaaatttta gcttctattt
      121 ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa taaaagatgc tgaatcgcta
      181 aaggttcttg caatgtgatc tctgctaaaa agtctggtcc ttctgtctct attttttggt
      241 cgattgagac ttcatctttt tgatcacgac gcttcagcgc gttctgcttc ctcaacagag
      301 aatagatttg attgcttata atgcttttat aataacttcc aaagctaaca cctttgcttt
      361 tttcgtaagt tctcaatgcc tgatataaag cgatccttgc ttcttgttcc caatcctcag
      421 aaacataatt tcttaaataa taggtgtttt tcaaacgtag taaaagtgga aaatattttt
      481 gatacagggc agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat aactcctcag
      541 acataaaata attcctccat aaattcgttt atggaggaat cttatcatca atgattcatc
      601 gaactgatta gaacgatatg ttatttattt tttctcatta aatcgtccaa tttttgggac
      661 aattttttta actgctcttc attccatgga gaattacgtc ggaaatcaat aaaatgcata
      721 tctttatttt cttgggctat tgttttttcc gtttttttta tcgtcttata taattcataa
      781 gcagaagttc gtaatgctcc ttgggaaaag atcatccact gttctgctaa atcgcttgtc
      841 gccactgtca cttgtgtcaa acggtcattc agctgaccag cgattctctc gatataactg
      901 tcagccgttt cgtcttcttt cgtaaaaaca acatccaact ggtatttctt ataattttgt
      961 tggatgcccg gaactagttg ggcatcgaag acaacgatga cttccattcc ttcatatttc
     1021 gcataatttg ataaacgatg taaaagagct tctcttgcat ccgccatttt gtcctgcttt
     1081 ttaagctgga caagttccgg ccacgaacca atcatgttgt agccgtctac aatcaacagc
     1141 tgttttttca tggtctactc cccctgacgt tgggaaaaag cctgatacat aagcaaacca
     1201 gcagcaacac tggcatttaa gctctggacg tgcccggtca tcgggatcgt cagcaattca
     1261 tccatttctt ttttcaatcc tggactcatt cctttgcctt cattaccaat gactaaagca
     1321 attgatcctt tggcattcca tttttggaag tttgtgccgg tcatatctgt gccaaatacc
     1381 caaaatcctg tttctttgag ctgttgcaca gcttgtacca aattcgtcac gcgtgcaaca
     1441 gggacatgtt cgactgcccc agtggaagtc tttgttacga taggtgtgat tccaactgct
     1501 cggtgttttg gtatgatcac accatcgacg ccacttgcgt ctgctgtccg taaaatcgac
     1561 ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc ttcttttttc
     1621 tcttttgttc gttcaatcag ttggtcaagg gataaatatt cataaggtgt aatcgcaaga
     1681 acgactcctt gatgcacgcc gtgatcgctc aaagtttcta atttttgttt aggaacccat
     1741 ttgactggca cagcttgttc acgtgcgatt tctttcagtt cttctacttt ttctcctcgt
     1801 gaatcttctt gaagaaagag tttattccct cgcccttgtt gtagcgcttc tactgcggca
     1861 tggtgcccaa agacgaaatt atcttctaat gcttcttcat tttgatcaga aactgactga
     1921 tttcgtccag tttgcttttt atttgaatat ctattttgtt ttttgtcttc aaatccacgt
     1981 ttcgattgat gtttgtttgt tctttcttta cccatttttt tcaccaactt tctgaataca
     2041 ccagcggatc aattcttcca tccgctcggt ttgttttgtc aaatgcagat agcccatgag
     2101 cgcttcgaac cctgttgcga cccgatacgt cgtaacatca gcattttttg cgctcgtatg
     2161 gcttttagca tttcttcctc tgcgataata aagttcttct tcttctgtca aaagatgttc
     2221 ttccagcatt tgctgaatca aaaaggcttg tgcattcgca gaaacataat tcgttgccgc
     2281 acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac gaacatagat
     2341 ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg ttcaattgtg tgtaatctct
     2401 catgcttctc ttctccatcg ggttccttgc ggtgtatctt ctaataagat ccctttttct
     2461 ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac gattgcttct tgcttgattt
     2521 cgttcttcga tcaattgttc aatctcttca tctaataact cttctgctgt aaataaaata
     2581 ccgaagatcg aaagccattg cgagaataaa gcttctcctt gtgccaagac agcggcagaa
     2641 actttcggct ggtctagata agtgttcagc cacttagcaa gctcatagac cactgtgatc
     2701 ccatttgctg cattaaaatc atcgtccatt tcttcgatga atcgctgttc taaattttct
     2761 aacaaagcga ttttatccgc atcatctgcc aaattctctg aggcagtttc ttggcggaat
     2821 ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc tttcatcgtc
     2881 gtttcactgt agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa acgcaagact
     2941 tgtggatcaa cgtttttgat catttcatgt acagtgatga aattccctag agatttactc
     3001 attttttcat cgtcttcgcc aatcgttaca tagccattgt gcatccagta attagcgaat
     3061 tttttccctg ttttcgcttc actttgtgcg atttcatttt catgatgagg aaattctaaa
     3121 tcttgcccgc caccatggat atcgatcgtt tctcctaaat gttttgttgc cataacggaa
     3181 cattcaatat gccaaccggg acgtcctttg ccccatggtg aatcccaaga aatctcgcca
     3241 ggttttgctt gtttccataa agcgaaatct aatgggtctt ctttttgctg ctgttcttcc
     3301 cctgtacgct gacttgctcc gacttccagt tcatcgatgg attggtcact taatttccca
     3361 taattttcaa atttacgggt acggtaatac acatcacctg aagattcata agcatagcct
     3421 ttttcaacca acacttgaat aaatgctaaa atatctggca tatgatccat gactcttgga
     3481 tgcagtgtag caggctcgac attcaacgct ttcgtatctt cttcaaatgc tcggatgaaa
     3541 cgatcagcaa cttctggtgc tgtaatacct aattcgtttg ctgtacggat gattttatcg
     3601 tctacatccg tgaaattcga tacgaaatcg actttatagc ctcgatattc aaaatagcgt
     3661 cgaattgtat cgaaagcaat ggtgctacga gcatttccga tatggatata attatacaca
     3721 gtcggtccgc agacatacat accgacatgt cccttttgta tagggatgaa ttcttctttt
     3781 tttctggtca atgtattgta gatttttatc attctgatct cctcactttt tctcctttga
     3841 ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc gtctggaaca tctttcaaca
     3901 caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct aatatctgcg
     3961 cattcgcaga gagcatggct ccttgtttaa cagttgggtg tcttttaccc gtttcttttc
     4021 ctgtgccgcc taatgttacg ccatggaata aaatcacatc gtcctcgatt tctgcggttt
     4081 cgccaatgac gattcccatt ccatggtcga tgaacacgcc agttccaatc gttgctcctg
     4141 gatggatctc aacaccagtt aggaaacgcc agaattgagc attgatcttc gccaataaaa
     4201 agagccggtg ctgatataaa aaatgagaga aacgatgcca aaacaaggca tgaaaaccag
     4261 ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg atcgtttctc tttgctgcat
     4321 caatcgctcg ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat tttatgtagg
     4381 aaatcatctt cttataaaga aaagaagcgc cttttaaata agtttcctta tcaaaagacg
     4441 ctttccgcgt ggttccactt ttcttcataa tcaatgatta tctctgacag ctaacgcctg
     4501 ttatacgttc ttggctactt tgtttcctat aaaacagttt ttcctgtttt ctgtgtttcg
     4561 ccaagaccac tcccagaggc atttcggcaa ccattcattg ataccttgca ccacccggta
     4621 tctctcttgt aatgatcagc tgcttacttt tttctgttca tcgtgtttag ttcagtactt
     4681 gatttaaatg tgctaatgat ttttcacgac caagcaattc gatcgtttca ggcaattctg
     4741 gaccatgcat ttgtcctgat acagctacac ggattggcat gaaaagattt tttcctttta
     4801 cgccagtttc tttttgaaca gctttgatgg cagctttgac agttggagca tctactactt
     4861 ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt tcaccagcaa
     4921 gtacttcttt tgctgcttca ttcaatacag gatgttcatt gaaaaatagc gtagacagat
     4981 caacgatttc tgcagcatag ctcatttgag gttgatacag gctaacgatc ttcttcaccc
     5041 attcgattgt cttttcatct gggttttctg ctacacgtcc atcagcgatt agataaggca
     5101 gacacatgtc agtaaatgca tctagatcca ttgctttgat gtaatggttg tttacccatt
     5161 gcagtttttt cgcatcgaaa gctgcgggtg atttacttag gcgttttgga tcgaacattt
     5221 tgatcaattc ttggtgttcg aagatttcgt cttcgcccac aggtgaccag cccaataatg
     5281 caataaagtt gaacattgca tctggtagat agcctaattc gcgatattgt tcgataaatt
     5341 gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg atgatcaatg
     5401 tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc atagatcatc agttgttttg
     5461 gtgtattggc aatatggtca tctccacgta aaacgtgagt gattttcatc aaatgatcat
     5521 cgaccgcaac agcaaaatta taagttggca taccatcgcg tttttgtatg acaaagtcgc
     5581 caccgatatt atcggattcg aacgaaatag cgcctttcac catatctgta aatgcgtatt
     5641 ctgtattgcg aggcacacgg aaacgtacaa cagattctag accttgtgct tctttttctg
     5701 cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt tctcctcttg
     5761 cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt gcaatagcat ttgtatgctc
     5821 gattgctagc aagcagctga tcaatcagcg gttgatagat ttctttacgt tcagattgac
     5881 ggtaagggcc gtattctcca ggattttcag gagattcatc ccaattcatt cccaaccaag
     5941 caaggttttc caactggctt ttttcgccat cttcgatatt acgtttttgg tcagtatcct
     6001 caatacgaat aataaagtct ccatcattgt ggcgggcaaa caaataattg aataatgcag
     6061 ttcttgcatt tccaatatgc aggtgcccag ttggacttgg tgcatagcgt acgcgtactt
     6121 tcgtcatttt cttcctcttc tttcaaacta gactttgact aacagcaaaa attccgtcag
     6181 ttacggcttc tcaaatatca tttatttaaa gccactcagc acaaatttta ccttttctaa
     6241 ggtaaatagt aaagccaact ggttgaattt ccttaccagt tggctgaatt tcttttttat
     6301 tttgattgtt tagtcttttt ctctgtctct tttttattgt cgattcctcg tcctgcatgt
     6361 gcaggtttag caaagatcat gcgtccagca gctgtttgaa gggcacttgt tacaacgact
     6421 tcgatacgtt cattcatgta atgctgtcca tcttctacga caaccatcgt gccgtcatca
     6481 aggtaggcta ctccttgttg gcgttctgtc ccggctttta ctactagaac attcatcgtt
     6541 tcaccaggga tgacgactgg ttttacggca tttgccaacg cattgatatt aagaacaggt
     6601 acattttgga attcagagac tttattcaag ttatagtcat tggttacaat gacaccatct
     6661 aaaagcttag ccaatttgat caatttgctg tctacttcac tgatatcttc aaagtctcca
     6721 tcgtacattt cgacagatat accctcttct ttttgcaagg catttaggat atctaatccg
     6781 cgacgaccac gcacacgttt caaactatcc cctgagtccg cgatgtattg tagttcgtaa
     6841 agaacaaaat tagggatcat caaggttcct tctaagaatc cagtctttgt aatatcataa
     6901 atccgaccgt caatgatcac gcttgtatcc aagattttat atttatggaa attttctcct
     6961 gattttcgtt ctaacagttg actctcaact tgactagctg tttcttgctc gatttttttg
     7021 cttcgtgaac caaaaacttt cttccattct tcgattcgtg tcgttcccat tctcaatccc
     7081 agatagccta agatgatcat cagtaaaatc ggcaatacac tattgacgaa tggaatttcc
     7141 atattgtaaa gaggggtcga gatcacaaca cctaaagtca atccgatgat cgcaccgatc
     7201 gcaccgaaga gaagataagt caagctgacc tcacttagtt tttgttctac ttgtttgata
     7261 atagctgaga tatgtttggc caacagcaaa gataataaaa agaaaataag tgcgcctaac
     7321 aggctgttgg taaatgtatt attcagccat ccgtttgact gttgaccgat tgcctgccaa
     7381 gctgagggta aaaatgaaat acccaagctt gcgccgacaa tgatcatcag caaagtaatg
     7441 acacgttttt gcatcgtttt tcctccgttt cgacaatcat ttattagtta gcggaacaca
     7501 cgcttcaacg tttcagccaa agttgctact ccaactatct cgattccttt tggcgcttcc
     7561 cagttaccta agttgttttt aggtaggtat acttttgtga agccgagttt ttggacttct
     7621 ttgactcgtt gttcaatatg actgacacgt ctgatttctc cagtcagtcc aatttcaccg
     7681 atgaaacatt ccgttggctg tgttcctttt tctttatagc ttgaagcaat actgacagcg
     7741 atagctaaat caatcgctgg ttcatttaat tttaccccac ctgcagcttt taagtaagca
     7801 tcttgattct gaagaagcaa cccagctctt ttttccagca ctgccatgat cagagatacc
     7861 cgattgaaat caagacctgt tgttgttcgt ttggcatttc cgaacatagt aggtgtgact
     7921 aatgcttgga tctctaccaa aattggacgt gtcccttcca ttgccacaac gatagcagag
     7981 ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg aaggattagc gacttcttcc
     8041 aatccatgct catgcatttc aaaaatccca atctcattcg ttgagccaaa tcggtttttt
     8101 actgctcgca aaatccgaaa actatgatgt tgttcacctt caaaatataa caccgtatcc
     8161 accatatgtt ctaacattct tgggccggcg atcgagcctt ctttggtaac atgaccgacg
     8221 ataaaaatgg ctatcccatt tgttttagcg attttgagta gttctgcagt ggtttctctt
     8281 acttggctga cacttcctgc cacacttgtg atgtctggct gcgtcatcgt ttggatggaa
     8341 tcaatgataa cataatcagg ggagatatgt tcgatggcac gactgatttc gttcatatct
     8401 gtttcagcgt atagataaaa ttctgtgttg atacttccga ggcgttccgc gcgtaatttt
     8461 atctgctctg cgctttcttc gccggataca tataagactt ttccgcctat ggctgctaat
     8521 tgctgagaaa cttgtaacag caaagtggat ttcccgatac ctggatcgcc accaatcaag
     8581 accatagacc ctggcaccac acctccgcct aacacacggt tcagttcttc taattttgtt
     8641 ttgatccgag gttctttttt agggacgacg tctgcgattt ttgttggttt tgctttttct
     8701 cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc ttcgaccatc
     8761 gtattccatt ttccgcaatt tgggcagcgt cccaagaact taggcgacac atacccgcaa
     8821 ttttggcaaa caaattgtac ttttgatttt tttgccattg taccgcctcc ttttatccta
     8881 atcatgagac attaaatcaa gcttaagtat ctttaacgtt ttatttactg gaagaaccaa
     8941 atccgccttg acgttgagca gtcgtttgat cttgatctgc ttttaaaaac ggcaaaaaga
     9001 tcccttggcc gatacgctcg ccttttttga tcactacatc cttgaaacca aagttggtga
     9061 attggaacat gatatggcct tcatttgcct cattattgta ataatcactg tcaatcacac
     9121 ctactccgtt tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg ttagcaagct
     9181 gcaaaaattc atcttctccc atgtaagctt tgattccagt aggcacgagg gttggtttca
     9241 attgtttttc attttctgat acatcttttt tcaatccatc tgcgactgtt tccaacccct
     9301 ttttccagcc tgctttccag atacttggaa tcacgacctc ttctgctgct tcaaaatcat
     9361 agccagcagc atgatgtgta gcgcgctgcg gtagattgat attttgatct ttatatttag
     9421 aaataacttc aaatcctctt tttctcaccg gttttcctcc ctaaataaac tactcctatt
     9481 ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa ttctgctatc
     9541 ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac aaaaaaacta
     9601 ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa tgagtggata
     9661 tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt attgtcaaag
     9721 taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca atcgatcagt
     9781 tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta gtctcatcag
     9841 gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact ttgattcctg
     9901 aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat aatcaaagat
     9961 tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg gtagattatc
    10021 ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg gacacgatcc
    10081 ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg acaaaatttg
    10141 gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac cttttgatcg
    10201 tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc gaggctcaga
    10261 tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt ggtgctggta
    10321 gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg atatttgaag
    10381 caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt gatattcttg
    10441 caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact tgatgcaatt
    10501 aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa accgcaaaaa
    10561 tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga tcattgaagc
    10621 aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga tgcaagaccg
    10681 cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa aaattgcttc
    10741 gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg gattgagaat
    10801 cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc gtccaaatgt
    10861 cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc ttcgcggtgg
    10921 caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag tactggtcag
    10981 tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg aaactgcagg
    11041 acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg gagcaaaatt
    11101 aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta caggaaataa
    11161 tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca tcgtcaatgc
    11221 aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc attctgagat
    11281 cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg tctctttacg
    11341 tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag acacagattg
    11401 ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt tggatgaagc
    11461 aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga cagatagtta
    11521 tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg tcaatgcctc
    11581 cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta tctctactca
    11641 aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa aatatatcat
    11701 ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc ccgccttttg
    11761 ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg aaattttaat
    11821 gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag tcggtatttt
    11881 atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa aatggtcgca
    11941 gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta gttacatggt
    12001 tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt caatcgctta
    12061 tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc agctgaataa
    12121 aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag agtgccttga
    12181 agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg tcacagcctc
    12241 tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt cctcttcgtt
    12301 aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct catccatgga
    12361 caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct gttcccgata
    12421 cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac cttccgcatt
    12481 gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg aaaattcaat
    12541 gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca tctattatcc
    12601 ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc agtttcatta
    12661 ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt ccgaataaat
    12721 ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca tgagaaactg
    12781 ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac ctcttggtaa
    12841 acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag
    12901 ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat
    12961 ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact
    13021 gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat
    13081 cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga
    13141 cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt
    13201 gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga
    13261 agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt
    13321 ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag
    13381 aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa
    13441 aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc
    13501 aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct
    13561 ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa
    13621 aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag
    13681 agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt
    13741 gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc
    13801 gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag
    13861 accagcgaat acaacgatga taaacaatcc tgtcatcaat gttttttcaa accgatccga
    13921 ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta actagtaaat
    13981 aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt taagattggg
    14041 tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac tagcgctgaa
    14101 cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt ctccaatatt
    14161 tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa atatgcacat
    14221 gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag gtaggatttt
    14281 tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac gttctgggta
    14341 tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga tctatacaac
    14401 gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg agcactctca
    14461 aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat ggatcaaaga
    14521 ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg ctttttcgac
    14581 gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc tgaacaaaat
    14641 catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac aagaatttgg
    14701 acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc aatggtgtca
    14761 gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa cctttctaga
    14821 aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg aaaagaaaaa
    14881 gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta gaactgcatt
    14941 caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg ttccatcatt
    15001 cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg atcaactgca
    15061 acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag aataattgat
    15121 caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc attcctaaaa
    15181 ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt actggtagtt
    15241 taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg aaagccacca
    15301 cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga tgcagccata
    15361 aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta aaaattattc
    15421 cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg aaaatcatcg
    15481 tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt tatttcataa
    15541 acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa aatcgttccc
    15601 ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat aaaattagtc
    15661 ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat cgtcagacgg
    15721 gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg agcaaaccct
    15781 aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct catgacaaaa
    15841 cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac ttgaaagctt
    15901 actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa taaggttgtc
    15961 ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa ctgaaaatca
    16021 ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc catgatggga
    16081 tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt catctgctgc
    16141 ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc attcttttgg
    16201 atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg tctgatttgt
    16261 tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt cttttcttgg
    16321 atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg tagctgagtc
    16381 tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa ctctcggatt
    16441 tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata gcgatcatga
    16501 ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg atcaatactt
    16561 tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata agaagaaaga
    16621 agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc tgaagtttct
    16681 tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc ttcagcgaga
    16741 taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc atgatcggga
    16801 aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt ttctcctatg
    16861 atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc tagctggttg
    16921 ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg ttttacactc
    16981 ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat ctgtttgcaa
    17041 aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa atcaccaaat
    17101 ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact ccatccatga
    17161 aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag ttcgaaacgt
    17221 aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc acacacgatt
    17281 caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac gtagtgatct
    17341 tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct attgataaaa
    17401 taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac tgatctgctt
    17461 cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac atgaattgca
    17521 tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt gaaaaactct
    17581 tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc atcttccctg
    17641 attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata tcgccaaaaa
    17701 tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc aaaggacgca
    17761 agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc atattgacag
    17821 aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt gggattttgt
    17881 atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa aaatcatttt
    17941 tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag ttgggtattc
    18001 aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc cggggatagc
    18061 acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg ggctttttct
    18121 gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga aacatagcca
    18181 tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg caacttttct
    18241 tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat ctcgtatcta
    18301 gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc ggctaatttg
    18361 atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt tctaataatt
    18421 gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt caaagaatca
    18481 gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa ttttttgtag
    18541 aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat tgattcttat
    18601 caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa aatttccgaa
    18661 atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc ttaaacagta
    18721 ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt gttcaaaaac
    18781 ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt tttgtttggc
    18841 tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata accataaaat
    18901 cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt agcactcaag
    18961 gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat tggcttggaa
    19021 ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa agagttgatc
    19081 tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa acggtagaca
    19141 ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc tacagttggc
    19201 agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg acggttatac
    19261 agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag attataggtt
    19321 tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg acctgttagc
    19381 caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga atagtagtaa
    19441 acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa tgattctttg
    19501 tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt cacatattcc
    19561 gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa tagattgtaa
    19621 ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc ttgagcaatc
    19681 atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga ggcaatacta
    19741 tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata gccagtatag
    19801 ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc atctgtcaat
    19861 tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga tgtactgtct
    19921 tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat tgtattttct
    19981 gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt ttctgaagaa
    20041 ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt tgtgttggca
    20101 actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt catctttttt
    20161 ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt aaaagcaagt
    20221 caatgtccat atttaaataa catttgtttc tttttgataa atttgctttt tctacataaa
    20281 aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt cgtttgaata
    20341 actatttaac aagtaattct ctttggatat tatccgttat ggattatact tcctgtgtcg
    20401 tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc gcaaatgcta
    20461 cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca tgaagttcaa
    20521 gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct cctggagaga
    20581 tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc atgattgcac
    20641 ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca tgtcccgcca
    20701 cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga agtacggcgg
    20761 aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat tcatcacggt
    20821 gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat acatcgattc
    20881 gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa tctcttacat
    20941 ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct tttaggcgat
    21001 cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc ttcgttgttg
    21061 cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat aaatcattca
    21121 ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc ttatttcatc
    21181 ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt cctgcaacga
    21241 tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg gcattctcgc
    21301 caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt ttcttgatgt
    21361 ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg ttcaccgtga
    21421 tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca cctaagtcaa
    21481 cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt ttgccgaaat
    21541 ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta atgatatttt
    21601 ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct gctaaaggct
    21661 cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt actcgaatct
    21721 ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct tttatcctta
    21781 ctttataacg gacggcttat catgtctaat acttataaaa catacttaac tattcctttt
    21841 aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat attcgtatac
    21901 tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca aaatctctgc
    21961 atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa cttggtgtga
    22021 cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga tatctttatt
    22081 caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta acaaatgacg
    22141 caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc tctttcattt
    22201 ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat ttatatagcg
    22261 ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt ggactcgtaa
    22321 tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa gatcgttggg
    22381 gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat ccgcaggaac
    22441 tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa ctagctgaat
    22501 ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta ttgtacaatg
    22561 cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg atcatcaata
    22621 cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct tcactgaatc
    22681 tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta gatgtcctaa
    22741 cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta acaacgaata
    22801 agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt tcttcctttt
    22861 atttctgata attccagtaa attactataa ttataaaaaa agaattgaca aaacataact
    22921 gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga tcatactagg
    22981 catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct atttttgttt
    23041 ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg gtgcgcgcat
    23101 gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg gacaggaaaa
    23161 agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga tttttgatcg
    23221 tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc aaaatttact
    23281 taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc atgcggaaac
    23341 tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc acattgcttt
    23401 ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa gagaaatcga
    23461 agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc tgacaaaaga
    23521 gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata ttcacagcgg
    23581 acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg tatctgacat
    23641 catccattta gtggaatcag aattggaaac agatatccat tcagaaccat tttcactgaa
    23701 ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg cgcaacaata
    23761 tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag aaagctataa
    23821 gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga tcacgaaaga
    23881 agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg tacaaacaaa
    23941 tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat gggacgttcc
    24001 cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat cggaaactgg
    24061 tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg taacgcagtt
    24121 ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc agtggacaaa
    24181 tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa tgttttaaaa
    24241 actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc atcttttgct
    24301 cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc tctttataat
    24361 cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa gcgtctagtg
    24421 ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct cgtatggcct
    24481 ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg ctggggtggt
    24541 gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca tcaagcaatg
    24601 aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt tttggcttct
    24661 gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat catgatgttc
    24721 gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa caaagcaaaa
    24781 acagcttcct tgatgctagc agcagctttt gcttcattct ttactggtgt gacagaacca
    24841 cttgaatttt catttatgtt cgttgcttgg ccattatatg tcttgcatgc aattttcact
    24901 gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc ctttagtgcc
    24961 ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc attgatgttg
    25021 atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt tgctatcaaa
    25081 aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga cgatactgca
    25141 acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc tgcattaggc
    25201 ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt gcaagtcaaa
    25261 gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg tatgaagatc
    25321 atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt tgcagatgag
    25381 atggcaaaac tacatggtgg tgctgcagca cgacctgcac aaacaaacac cgtggttaaa
    25441 acagaaacat ttgccggtga aacgatcgaa caagatattt acgctatcgc aaatgggaaa
    25501 ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat gggtgacggc
    25561 tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac gatcacaaac
    25621 attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga agtcttgctt
    25681 catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata tgtagaagaa
    25741 ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc tattcaatct
    25801 gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt ccaaagctta
    25861 gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt ttccaacaaa
    25921 gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag ctgagacaaa
    25981 aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa ctccgcggta
    26041 ataagtccag ctaaacgaaa catatgaaga gctatttttt atttgactct tcttattact
    26101 tgatgcagaa cagctttttc acaaaatttt tcttcatact caaaactaat cggctttttc
    26161 acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct tcaagagaag
    26221 ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac tgatcctttt
    26281 taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat ccggcttctt
    26341 ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaactct ttacctgtcg
    26401 cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat aattctaaga
    26461 tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat tcacgtttca
    26521 atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt tttaaagcct
    26581 gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat gtatgggaaa
    26641 tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat acactgatcg
    26701 agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct gcttgtgctg
    26761 ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag acaaaaagca
    26821 atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa tcaccaatac
    26881 gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt actagtacat
    26941 agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca ccaaaacctc
    27001 cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt tatttgttaa
    27061 gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg taagataagt
    27121 caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt tagtgatgca
    27181 atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc tagtgagatg
    27241 attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag tcagctcaaa
    27301 aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc caaaagtcca
    27361 gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca gcttatccaa
    27421 gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga agctttagaa
    27481 gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat cacacttgat
    27541 agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa cacaggcctc
    27601 gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg gaatcgtcta
    27661 cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt tcctctctaa
    27721 tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa gtagctgacc
    27781 ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt ttcctctcta
    27841 atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta ttgactaccg
    27901 gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact acagcatttg
    27961 gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag gccgtattcg
    28021 tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gcttacaaca agatacgttc
    28081 cttttgggaa tgggatgatt cgcgtagtgt ttggaacatc ctgactcgtt tgaacaccga
    28141 catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa tgattttcag
    28201 cagcttgttt caatgcatct attgttccat taccgataac tttttgctca aatgcttgtt
    28261 tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca ccaatacccg
    28321 tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc accctttcta
    28381 ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg ctcttttttc
    28441 tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa atctcactgt
    28501 aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct tcgactaacc
    28561 cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct tgtaaagttt
    28621 ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata gcaagcattt
    28681 ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc atcaaactgc
    28741 ccttttcttc caagcggtct ccatcaaatc cataagaaac gttgattact tgatttggat
    28801 ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc tgcgctaact
    28861 tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc acacctgcca
    28921 cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct ctgacatcaa
    28981 taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctactgatt tctgaaggca
    29041 gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca tatccatatc
    29101 taaacgccgt ttctgtcact cttgatccat ctagaagatc gcgattagcc aaagccaatc
    29161 tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag atgcgtttta
    29221 attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt ccttctgtca
    29281 aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt atcttcctcc
    29341 ttgattgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat caggaaccaa
    29401 tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc agcgtttcgt
    29461 aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat aaattttggg
    29521 gaagacagat tctaagtaaa caaagcggta aaaatatctt ttattccctt agttaatccg
    29581 cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa cgctgcttgg
    29641 atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt tatttctaac
    29701 aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc tttaaaacac
    29761 gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg aatttttgac
    29821 aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct taaacgtttc
    29881 tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata attggcttga
    29941 gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg gttttgtccg
    30001 tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg aagattataa
    30061 ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat atgccataga
    30121 aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac gtccaataaa
    30181 aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta gttcctctct
    30241 tgtacgggag cttttaacag agattcttat agaacatccg cactttatcg aacgcttcaa
    30301 agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta tcatccataa
    30361 atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca tcatggagat
    30421 gcgaaagttc atccaagaag cagttgaatc gcttattcag caaaatgcct atcttcccgc
    30481 attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg agcctgagga
    30541 tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat tattagcaga
    30601 agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag tcgatcatac
    30661 tgacgcctct tattcaaaac gctactctat aaaaatcctg aaagaagact ttcgggaaaa
    30721 agaattttct aaccaaaaga aaaaaataga taaaaaacta aagaaacggt aaagaaagac
    30781 gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac aagaaataca
    30841 gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag acttaacgcg
    30901 tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt catgacaaat
    30961 ttccttcgtt gctggataga ttttccggca ttgttgatag attttatgat acttttctac
    31021 attttgtaag agaggttccg ctgcatctgt atattggata aaattctgac aacaactttc
    31081 aggtgtgtcg aaccacccta accctagtgc agcaatcatt gttgcgccta atgcaggtcc
    31141 ttcctccaca cttaaacaat agaccgttgc atcgaaaata tcagcttgga tttgtagcca
    31201 tgcaagattt ttcgcgcctc ctcccaccga aacaatacgt gtaaatctgc gacccgctag
    31261 ttctttacgt ttcaatccca ccattgacaa atgctcatta aaaatttaga atattcacaa
    31321 gtttaacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat tggatgaaaa
    31381 gttagatata aaaactgcga tcaaagacac gttacctacc gttttcggtt atatcggtat
    31441 tggacttgca tttggtatcg ttgggaaagc tgccggattt catccactag tcgtcacgtt
    31501 gatgtccctg ctggtctatg ctggttctgc ccaatttatc acagtcagca tgcttgctag
    31561 tcacagccca ttgctttcca tcgttttctc gacctttcta gtcaattccc gaatgattct
    31621 gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa atctgctgat
    31681 tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga actatactgg
    31741 acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt tgacgtgggt
    31801 gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga aaaagtttgg
    31861 tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag tcatttctga
    31921 tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga ttctggtata
    31981 tgtcggactg atttttatcc caagcaactt agtgattgta gttgtaacac tgattgggtg
    32041 cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg actattttag
    32101 gttgtacttt agcaacttgg ctttctaggg tcctaccatt tgtcttactg aaaaaattcg
    32161 atttaccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt atgtctgctt
    32221 tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc aatctcgaaa
    32281 atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc ttattggtga
    32341 tcgtcttggc aggaattctt tccctttcgt tgattcgctt attataaata aaaaaacaag
    32401 agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat aagaaaaaaa
    32461 tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc ctaacatatg
    32521 tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc catctgaata
    32581 gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa tgtgcgctaa
    32641 agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca tatactccca
    32701 gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt tgatccctac
    32761 gatcgttccc acatgtccaa atggtcggac aaggtgcttg atttgatcaa agtagcgggt
    32821 aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac gaaaactttt
    32881 acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca gccattcaaa
    32941 attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta gttgattcaa
    33001 aatcgaaccg acgcccccgg atccgttgat cactaagatt tttttcccgc gattggcatt
    33061 ttcttctgga gttagaccaa atttttcaaa taacaattca taagcggtca atgaggtcaa
    33121 cggcaaggct gcagcttctt catctgaaag attttttggt gcaagagcga caatacgttc
    33181 gtcaactagt tggtattctt gattgcttcc tgctcttgtt gttgtacctg cgtaaaaaac
    33241 gcgatcgccg acagaaaatt tttgcacttc gtcacctaca gcaacaactt tcccgactcc
    33301 atcaaaacct aagatacgta aagcatttct tatcccgtta tcttggcgaa gtttcgtatc
    33361 gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac tgggtacagg
    33421 tatctgttct ttttcatcaa taaagctttt tggatcatcg atcggcaacc cttcataaaa
    33481 accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc ttcatcaagt
    33541 tggagcatcc atacagtgtt catttcaata tatatagtat gctatttgtt ccaccttttt
    33601 tattcatcat aattattctc ttacttaaag taaactattt gcactttttt ggttaaaaaa
    33661 gaggttgcga caagactttt gtcacaacct cttataaacg gtgttctcct accaactctt
    33721 cggtcacaag tcaaaatatt catcaaacat gagaagctgt tttttgaaat tgctccttgt
    33781 gactcagagt taaacggcag gctcacaacc tctgatataa acggtgttca atcaactgac
    33841 cctcggtatt agctcaaaaa acgacaaaat atgaagagct atttttgttt ggctgttctt
    33901 attacttgat acaggacagc tttttcacga cctctaattc acggtgttca agcaagaaat
    33961 cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tctctaagat ctctcgtact
    34021 tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc tggaaatcct
    34081 ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat gatttctgaa
    34141 taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt ttctgtatgt
    34201 tccctcgggt ctttttcaaa tgcataagga ttcttgaaaa tccctcggcc aatcatgatg
    34261 ccatcaacgc catatttctc ggccaattct tctcctgtct ggcgatcggg gatatctcca
    34321 ttgatcgtga ttagtgtttg tggagcgatt tggtcacgta aagcaatgat tttaggaatg
    34381 acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg gacagaaaga
    34441 ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga catttccgta
    34501 tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagctgc ttcaatcaat
    34561 tgtgccgcta cttctggacg aaggatcaag ccactgcctt ttccacgttc tgctacatta
    34621 gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc catagcaatg
    34681 ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat cggctgttca
    34741 tcttctgtaa atgttaatct tcctcgtaca ctgtcttttc catctggatg acagaaactg
    34801 tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt tacgacatgg
    34861 cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc
    34921 tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag cagtgaagtc
    34981 gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag caaagtgaac
    35041 agcttaaaag atgtttctga ttgagcaaaa aaaagactgt gacaaaaatc ttatcacagt
    35101 ccattttccg gaataaatgg tgttcaaaag cttgcatttg cggtaataag tccaaccaaa
    35161 cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt
    35221 tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt cgctcaaaaa
    35281 acaacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg tcaaaacgct
    35341 gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca aataccaagt
    35401 aacggcgtac aaaacaacga tgaataacac aagtacgatc atcgatgttg ctactggcac
    35461 atcgatcgtt ccaaggaatc cgtaacggaa accggagatc atataaacga tcggattgac
    35521 ttttgaaaca gcttgccaaa agggcggtaa catagagatg gcatagaaaa cgccacctag
    35581 ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcatcat aagactgagc
    35641 aaaaatcccg ttgagcagtc ctgctaatga aaacaagatt gccgtcatca ataatgtaat
    35701 gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa tgatcgttac
    35761 gagagagccg acgagaatac tgcgacccac accaccaatc acaaagcccc aaataatcac
    35821 atgcgtagga acaggtgcta ccaagatttc ttcgatattt ttctgaaatt tttgagagaa
    35881 gaaggacgaa gaaacattgg catacgaact agtgatagca gacatcataa tcaaaccagg
    35941 aacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgcccac cgatcatttt
    36001 tccgaaaata acaaaataca aggatgtcgt gatcactggc ggaaccagtg tttggaccca
    36061 tatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag taaaataaag
    36121 attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaag aacagttctt
    36181 ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt gtaagctgat
    36241 cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc tgctcatctt
    36301 caaatgtaca agcatatcct tcaatcacag gtttggtatc atatggcgct aaatcaaaga
    36361 taaatgtttc aaattgcagt tttgacagaa gctctttcat acttgtattc tcgatcagtt
    36421 cacccgattg gatgattccg atgttgcgac aaagcatttc agcttcttct agataatgcg
    36481 tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg tagaaactcc cacatctcgc
    36541 gtctcaactc gatgtcaact cctgctgttg gttcatccaa gatcagcaaa cgtggttcat
    36601 gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc atacgtgcac
    36661 ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg cgtttcatcg
    36721 cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc tggaccgttt
    36781 caaaagggtt aaaattgaac tcttgtggaa ccaatccaat ctgttgcttt gctcgaacta
    36841 gatccttgtc cagatcataa ccgaagacat tcacttttcc agatgtttta ttgactaaag
    36901 aagtaataat cccgatcgtt gtagacttcc cagctccatt tggccctaat agcgcataga
    36961 aatctccttc tttcaccgaa agatcgattc cccgcaatgc ttcgacccca cttccataga
    37021 tctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct ccttatagac
    37081 atgattctta attgtatcat ggaatacagc agatgaaaaa acaacttggc ttccacacaa
    37141 aaacacacct gctaacaaag aggagatgtg ttttctagtt catgatcatc ttcgatagaa
    37201 taactatcta aacaactctg gaacttttaa aatcataaaa gtaccgacag caactactac
    37261 gatgacatat agaagatcct tataactgag gttcttgtct tttttattca tgttaagctt
    37321 tcctccgtcc ttttatcgaa agcttttttc ttttatttta accgaaataa ataaaatgga
    37381 aatagttatt ttcaatttct gttttcgctg tttgttttac ggagaacttt gccatggaac
    37441 gtgcgtctcg tcgctgatgc taagtttttt cgataaaagc tgctagaagg aagacagcgt
    37501 ggtttatttt gtatcgtctt ttgctaaatc ttccaagatc atctggacag cagactcgtt
    37561 cccattgaat tctgccactt ttttggcatc aatgtacttc ttttgataat cgtctttttg
    37621 gacccacgca tcatgcttcg tttgataaag ttaatcaaac ggataccact gacgctgttc
    37681 atcgaacaac aaactttctt tttgggcctg ttcaactatg gatttaggat aattcaaaac
    37741 tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt tgaaatcaaa
    37801 actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac aataattctt
    37861 taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg gatactccgc
    37921 aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg ttccttttaa
    37981 aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca gcaaacgttt
    38041 gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat cttcgattgc
    38101 catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg ctgtttggat
    38161 cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt ttcctgatgc
    38221 attggaaccc gtgactaatg tattcttctg ccagttgact ggattaacta ccgcttcttt
    38281 taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact ctggtcggca
    38341 agtgatcggc atataggtgc gataattcaa aacggctgct gcaacttcca attctcccag
    38401 caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga gaacgaaatg
    38461 ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca attctgcttc
    38521 actcccgctc ttcattcgaa atgaaaaacc taatttaggg atgtctctta atggtttagt
    38581 ttgctgtttc agctcttttt gaagtggtgt cgtcattttt gctaactgat tggcggctga
    38641 aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt gtttcttcac
    38701 tagataatag accgtattga atactaagcc accgattgct agatagattc ctagtacttg
    38761 ccctaaaaga agcaaaccta cacccaccag cggaagcaag ccaagaaaaa taaataaacc
    38821 tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa aattatgatc
    38881 tttttttcct aaacgagcga attgatattg gattctttct ctcagttgag gattttcctg
    38941 ataaaaagca attagttttt ctaactgata atcttctcca aaatcaaagc tacgtaaacg
    39001 ctgatacaac gcttctgaac ctacactaga ataggtactg ttgatccctt caaatacttc
    39061 gaacatatcc aagtcatacc aagtaagatc gtcgatttca ctatcccatg aacggaattt
    39121 tttctccgtg agccacgcat ccttcaaact ttcttcttta tcaaatctcg gttgataagg
    39181 aatttttccc caattactac gaactgtttc ctttactttc aaacgtgaaa aaatgtctaa
    39241 aacaactaat aaaataacaa ctgcaataat aattaaaacg ataaattctc tttggcccat
    39301 atccctctcc gttccttgat ttgttagttt tagtatagca ctgttgcgct atttgaaaag
    39361 ggggtacaaa aataactgtc ttcgttacct aattcgtatc aatcacacca aaataaaaac
    39421 accttccaaa aaagaagatg tctttaaaac gagatacaat tttttgtcat tcacttcagg
    39481 taaattagag ccaattaaac cctccgcaac ttgacggaga tggagaagat gcccccgtct
    39541 aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag gtaaagcccg
    39601 attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg ctggaagact
    39661 ttttcaacag aggtttgcgc tcctggtttg ataggattgg ccactgttaa tcgaatgaat
    39721 gaatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa taaattctgt
    39781 atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt tgtatttatc
    39841 gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata aaaagagaaa
    39901 aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc tttttgcttc
    39961 gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct tgcaacagat
    40021 tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg ttccagtaat
    40081 tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag gccgacgatc
    40141 gatagtctga tgttttcttc catttcttga aatgcttgtc ctttttgttc attttcatcc
    40201 acccatgtat tcagtgcatc tactaggtcc attagattct tttctcgaaa ttcgactgaa
    40261 agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt taatctcttg
    40321 atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag aagcaacagc
    40381 cacttaatca aatctaaacc ccactcccca caccgtatga atatacacag catgtgcctt
    40441 tgctagtttc cctcgaagat tggaaatatg catattcact gttttctcac tatcaaaata
    40501 tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt tcggatgttc
    40561 taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagcca caccattcac
    40621 atttacatcc ctcgactctt tgtccagtaa gatttccttg taatagatgc gttgcgacag
    40681 atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta acaacagttc
    40741 ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca aggaaacttt
    40801 atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg tctgtcggat
    40861 ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa ggatcaaatc
    40921 gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat caagaagttt
    40981 gtatctctgt tcaagaacct cctccagtaa agaatgaaca gcttcatcgt cttcgataat
    41041 caaaatagtt tccagcagga acacctactt aatgtcagat ttttggaaaa taaacattcc
    41101 tagagctgat gataccacga ttgttactag actgaccaga atcattttcg gtgcttgcga
    41161 ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa accactccgt
    41221 tagaaattcc aagcctttga tttttgcgaa aacaccggga acgaaggccg ttcccatgat
    41281 ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg tcaaagccac
    41341 tggaacagat aatgtcgcta atatcagcag aagcaataca ggaaatactt ttagaacata
    41401 atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg tgtaagcaat
    41461 catggctagc cccgtaatca aaaccacgcc aagtgttgtg atcaaaagcg taacgagaag
    41521 tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc gaatcgtacc
    41581 gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca gaagaatcca
    41641 tgcagccatt ttttccacca ttaaaatccc tgttgcaccg ttgatgactg tatccgttgc
    41701 ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag aataaaaaag
    41761 cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc atacttttat
    41821 tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt aagaaataat
    41881 cttccagttt ttgcccaact aggtggatac catccacagg aacttttgcc tctaccaatg
    41941 cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca cggctagatt
    42001 ggacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt tccgtcgccg
    42061 aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc gataattctt
    42121 tgatcaagtg tccttcatgc aaaataccgt agtgagtggc gatctgtgac aattcatcta
    42181 gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt tggatgattt
    42241 ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc aaaatcaaaa
    42301 attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata cctaaagaga
    42361 aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc aggtcttcga
    42421 tttctctatc cgacacaccg ccgttcgctg cttggatacg taggttatct cttgcagtta
    42481 attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt gctgcctgca
    42541 aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa cccataatag
    42601 ttctcataaa ggtagactta cccgctccat tctccccaat caaaccatag atcattccac
    42661 gtttgatttc gatcgatact ttatctaatg ctttggcagc accgtatttt ttggttatac
    42721 cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt tcttattgat
    42781 aagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa gaaagtgtaa
    42841 attttccaat tgaatttcaa gaaaaatcag actgtctcgc agacgtggtt gagttgtctg
    42901 ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca tggtaatcgt
    42961 tgttatcgtt gacatcagac gtccttgtat atgcgttgga acttctcgct gaagccagac
    43021 catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa aataatacat
    43081 gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga cttttcacaa
    43141 tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
    43201 tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc tgaccacttc
    43261 tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc tttttcccgt
    43321 ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca tagctaaaaa
    43381 cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat taaggtaact
    43441 gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga acgataatcg
    43501 caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg gttttttctt
    43561 cgtatcattg aataagtttg aaaagccttg atttataaag gtttttccg
//
LOCUS       DADXTH010000012        43138 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.012, whole genome shotgun sequence.
ACCESSION   DADXTH010000012 DADXTH010000000
VERSION     DADXTH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.012"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            75..1073
                     /locus_tag="IWI97_000642"
     CDS             75..1073
                     /locus_tag="IWI97_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462171.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(1313..1396)
                     /locus_tag="IWI97_000643"
     tRNA            complement(1313..1396)
                     /locus_tag="IWI97_000643"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1360..1362),aa:Leu,seq:gag)
     gene            1451..1630
                     /locus_tag="IWI97_000644"
     CDS             1451..1630
                     /locus_tag="IWI97_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462172.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1779..2996
                     /locus_tag="IWI97_000645"
     CDS             1779..2996
                     /locus_tag="IWI97_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBC2462173.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3180..4016
                     /locus_tag="IWI97_000646"
     CDS             3180..4016
                     /locus_tag="IWI97_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2462174.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4092..5240
                     /gene="nagA"
                     /locus_tag="IWI97_000647"
     CDS             4092..5240
                     /gene="nagA"
                     /locus_tag="IWI97_000647"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBC2462175.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5461..6519
                     /locus_tag="IWI97_000648"
     CDS             5461..6519
                     /locus_tag="IWI97_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBC2462176.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6523..7557
                     /locus_tag="IWI97_000649"
     CDS             6523..7557
                     /locus_tag="IWI97_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBC2462177.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7659..8375)
                     /locus_tag="IWI97_000650"
     CDS             complement(7659..8375)
                     /locus_tag="IWI97_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBC2462178.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(8344..9129)
                     /locus_tag="IWI97_000651"
     CDS             complement(8344..9129)
                     /locus_tag="IWI97_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBC2462179.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9120..9869)
                     /locus_tag="IWI97_000652"
     CDS             complement(9120..9869)
                     /locus_tag="IWI97_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBC2462180.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10167..10691
                     /locus_tag="IWI97_000653"
     CDS             10167..10691
                     /locus_tag="IWI97_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBC2462181.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10707..12206
                     /locus_tag="IWI97_000654"
     CDS             10707..12206
                     /locus_tag="IWI97_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBC2462182.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12294..12896)
                     /locus_tag="IWI97_000655"
     CDS             complement(12294..12896)
                     /locus_tag="IWI97_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBC2462183.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            13000..13956
                     /locus_tag="IWI97_000656"
     CDS             13000..13956
                     /locus_tag="IWI97_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBC2462184.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14261..15217)
                     /locus_tag="IWI97_000657"
     CDS             complement(14261..15217)
                     /locus_tag="IWI97_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2462185.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15391..15951
                     /locus_tag="IWI97_000658"
     CDS             15391..15951
                     /locus_tag="IWI97_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462186.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15944..16768
                     /locus_tag="IWI97_000659"
     CDS             15944..16768
                     /locus_tag="IWI97_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBC2462187.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16778..17395
                     /locus_tag="IWI97_000660"
     CDS             16778..17395
                     /locus_tag="IWI97_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBC2462188.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17413..18096
                     /locus_tag="IWI97_000661"
     CDS             17413..18096
                     /locus_tag="IWI97_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBC2462189.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18779..20062
                     /locus_tag="IWI97_000662"
     CDS             18779..20062
                     /locus_tag="IWI97_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2462190.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            20078..20965
                     /locus_tag="IWI97_000663"
     CDS             20078..20965
                     /locus_tag="IWI97_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2462191.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20982..21818
                     /locus_tag="IWI97_000664"
     CDS             20982..21818
                     /locus_tag="IWI97_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2462192.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21821..22444
                     /locus_tag="IWI97_000665"
     CDS             21821..22444
                     /locus_tag="IWI97_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462193.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22441..23733
                     /locus_tag="IWI97_000666"
     CDS             22441..23733
                     /locus_tag="IWI97_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2462194.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24092..24189
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24305..24886
                     /locus_tag="IWI97_000667"
     CDS             24305..24886
                     /locus_tag="IWI97_000667"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBC2462195.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24889..26196
                     /locus_tag="IWI97_000668"
     CDS             24889..26196
                     /locus_tag="IWI97_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBC2462196.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26612..27100
                     /gene="purE"
                     /locus_tag="IWI97_000669"
     CDS             26612..27100
                     /gene="purE"
                     /locus_tag="IWI97_000669"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBC2462197.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27093..28217
                     /gene="purK"
                     /locus_tag="IWI97_000670"
     CDS             27093..28217
                     /gene="purK"
                     /locus_tag="IWI97_000670"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBC2462198.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28230..29525
                     /gene="purB"
                     /locus_tag="IWI97_000671"
     CDS             28230..29525
                     /gene="purB"
                     /locus_tag="IWI97_000671"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBC2462199.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            30052..31440
                     /gene="ltrA"
                     /locus_tag="IWI97_000672"
     CDS             30052..31440
                     /gene="ltrA"
                     /locus_tag="IWI97_000672"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBC2462200.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(31604..31981)
                     /locus_tag="IWI97_000673"
     CDS             complement(31604..31981)
                     /locus_tag="IWI97_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462201.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32237..32965
                     /locus_tag="IWI97_000674"
     CDS             32237..32965
                     /locus_tag="IWI97_000674"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBC2462202.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            32965..33219
                     /gene="purS"
                     /locus_tag="IWI97_000675"
     CDS             32965..33219
                     /gene="purS"
                     /locus_tag="IWI97_000675"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBC2462203.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33221..33892
                     /gene="purQ"
                     /locus_tag="IWI97_000676"
     CDS             33221..33892
                     /gene="purQ"
                     /locus_tag="IWI97_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBC2462204.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            33893..36115
                     /gene="purL"
                     /locus_tag="IWI97_000677"
     CDS             33893..36115
                     /gene="purL"
                     /locus_tag="IWI97_000677"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBC2462205.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36100..37539
                     /locus_tag="IWI97_000678"
     CDS             36100..37539
                     /locus_tag="IWI97_000678"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBC2462206.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37562..38614
                     /locus_tag="IWI97_000679"
     CDS             37562..38614
                     /locus_tag="IWI97_000679"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBC2462207.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            38611..39189
                     /gene="purN"
                     /locus_tag="IWI97_000680"
     CDS             38611..39189
                     /gene="purN"
                     /locus_tag="IWI97_000680"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBC2462208.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39186..40721
                     /gene="purH"
                     /locus_tag="IWI97_000681"
     CDS             39186..40721
                     /gene="purH"
                     /locus_tag="IWI97_000681"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBC2462209.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            40737..42011
                     /gene="purD"
                     /locus_tag="IWI97_000682"
     CDS             40737..42011
                     /gene="purD"
                     /locus_tag="IWI97_000682"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBC2462210.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42133..43047
                     /locus_tag="IWI97_000683"
     CDS             42133..43047
                     /locus_tag="IWI97_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBC2462211.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT        13854 a         7391 c         9493 g        12400 t
ORIGIN      
        1 atgtttttga gaaaataaat gttaaaaatt tattattggt ttgctctata tactgtttaa
       61 taccattgac agtaatgggg gaaaccggtt ggagagcaac caccttgaac tatcagtggc
      121 cagtagcatt tagtttacta actttttatc cgttttttca actcttaaga ggagaagaaa
      181 taaatagaaa aatatattgg gtaagtattc ctttattgat atttttaact aatcaagaac
      241 aagtcaacgc ctgttttttt gttctaacaa gtatagttag tttatatttg attgtcaatg
      301 gacgatataa ttataagtta tcagtatttt caataataag cttagcagaa ttaattttct
      361 ctttgacaac tccagggaat gcattacgtg ctgcacatga aattaataaa tggtttcctg
      421 aatataaaaa tttcaacttt cttaataagt tagatttagg tatttcatca tttggaaagc
      481 cgtttttttt agacatgaat atcttatttt tattgctatt tttcttaatt ttccttctaa
      541 catataggaa atgtcaaaac tattatgtgc gtatattgac tgcattaccg tttttcttaa
      601 acctgattat ttattttggt aatacaatgg gtcaaagctt tacttacgtc aatggaaata
      661 aaagggcaat gatttggagt agtagtaacc tgaataatct ctttacagag ttaggaacga
      721 aactgtcact tttttatcca ggtacttgga tagctacttt agtagtcttg gcattattac
      781 tatgtttaat tgttggaatt tatttaagtt ttgacaataa gaaaacttct atttttttag
      841 ttattttgat gataatggga ttttgttcaa ggttaattat gggattttct cccacagtat
      901 gggcttccgg aatgcggacg tactatatac tttatgttgt tatcgccatc ttagtactaa
      961 tggcagttaa agagctaatg aaaagcatga gtgtacaaaa aaatgaattt atgcaatttg
     1021 gattaaccgt gcttggtatt tgtaccttta taataacggt gataaacaga taatatacaa
     1081 aaaatccctc gctttagtat taggtgaggg attttactcg tttttcgatt aattgtttta
     1141 acacttgcaa gtttctacga gtagcttgtt tccgaccttc atatactcac ctcaggctgt
     1201 ttacatgatt gtacgttctt tttgtaaagg atacagtgat atgtatataa tggcataaag
     1261 aaacgttcaa acaaaaaaac tcgtgaagca aaattaactt cacgagttgt agtgcagatg
     1321 atgggactcg aacccacacg agctaacgct cacatgaccc tcaatcatgc ttgtctgcca
     1381 attccaacac atctgcgtaa taataacaca ctaatgatag cgaggtttta catagttgtc
     1441 aagctcaaaa atgggaggac ttattaagat ggagaaatat gacggagaat tttcaggact
     1501 gggaatgatc ttaggtgtat tgattggact tgcattcgga cgttttttgc ttggttttat
     1561 gctaggaatc atttgtggaa ttgcaatgga ttgggcagcg aatctatgga atgactatca
     1621 tgacaattaa gttctcaacg cttgtttgtg tttttttaat acttacgttt ttcctaaata
     1681 agtggcaaaa ttttctagga tggagtatga tattgaaata gtaaataaca aaaaatttgg
     1741 aaagttctaa ctttttgaaa tgagggattg gctaagtgat gagagaattt gaaaaatcca
     1801 acaaattaga aggtgtcagt tacgatgttc gcggtcccgt tctagaagaa gcggagcgga
     1861 tgcaagaaga gggcattagt attttaaaat tgaacacggg aaatcccgca ccttttggat
     1921 ttgaagcacc aaacgaagtc attcgtgatt tgatcatgaa tgcccgtgac tcagaaggct
     1981 actcagattc caaggggatc ttttctgcta gaaaagcaat tgagcaatat tgccaactga
     2041 aacattttcc gaatgtgacg atcaatgata tctacaccgg caacggagtg agcgaattga
     2101 tcacgatgtg tatgcagggg ttgctggaca atggagatga agtactggtt ccaatgccgg
     2161 actatccgtt gtggacggct tctattgcat tagctggagg cacacctgtt cattatattt
     2221 gcgatgaaga ggcagaatgg tatcctgata ttgatgatat caaatcaaaa ataacgagtc
     2281 ggacaaaagc tattgttatc attaatccta ataatcctac tggggcactt tatccaaaag
     2341 aattattaga agaaatcgtt gaagtagctc gacaaaataa cctgattatt tattccgacg
     2401 aaatctatga tcgcttagtg atggatggtc tagaacatat tccgattgct acattagcgc
     2461 ctgatttgtt cgttgttacg ctgaacggtc tatccaaatc tcatcgtgta gcaggatttc
     2521 gagtaggctg gatggtccta agtggagata agtcacacgt caaaggctat atagaagggc
     2581 tgaatatgtt gtcctccatg cgtttgtgtt ccaatgtatt gtctcagcaa attatccaaa
     2641 ccgcgctagg cggttatcaa agtgtcgatg agctgctcct gccaggcgga aggatctatg
     2701 aacaaagaga atatatctat aatgcaatca atgatattcc tggtctttca gccgtaaaac
     2761 caaaagctgc tttttatata ttcccaaaaa ttgatacaaa gcgattcaat attttggatg
     2821 atgaaaaatt cgttttggac tttttacatg aacatcatat tctacttgta catggtggtg
     2881 gatttaattg gaaccagcca gatcattttc gaatcgttta tcttcctaaa atggatgatt
     2941 tgaaacaaac agctaagaaa atgagagaat tcctagcaac ctatcgccaa aaatgatttt
     3001 aaaaaccaaa tttttcagta ggagggccac agacaaacac actgaggctc tctttctagt
     3061 ggagttttct taaataacta aagtatatta tttagttgca agtcttttct tgaagtttgc
     3121 gacttcctaa agtataatac gaatagaatg cgaatacgca tattttgggg aggatactta
     3181 tggaaacgtt aatcgagtcc tttggaacga tcattaaaga gattcgaaaa gaacaaaaaa
     3241 tgacgcaaaa aatgctttcg catgatattt gttcacaaag tgtattaagt cggatcgaaa
     3301 ataatgagga actgccgaat gtattggtga tgatgcagat ttgtcaacgg ctaggggtaa
     3361 cgattgatca tgtgatgaat ctctctcgaa aaactactcg gacaaacgat aaattatttg
     3421 aactgttgga tagttgtttc caaaaacgtg attatcggaa gttagaacaa gtactaaaat
     3481 cttctgatat ttctgaaaat ctgtatcaag caactgactt tcaacgctat tattattatc
     3541 tcggtgtttg cgaattcacg ttgcgtcaaa atatctctca gtccctttat tatttgaaag
     3601 aagcattaag ctactctagc caaaaagaaa gaatccatgt ttctgacaca gaaatacaac
     3661 tgattagttg tatcggaaaa atatatggtg tggcaggaaa aacggctgaa gcacgctact
     3721 atttgagtcg aagcatccaa ctgctgcatc aatcagctga tgaacgatca aaaagtgaat
     3781 tttcgcaaat tttttataat tatggaaatt tcttattcca tcaaaatgaa atcgacgaag
     3841 cgttagaaca ggtgaatcaa ggaatctatt gggctcaaga gaagaatagc tactattacc
     3901 tcaatgattt atttgtgctt aaaagcttaa tatacaagcg taaagaagaa ccagaaagag
     3961 cagcattcta tgaaggctta gcacaagctg tgaaacaaat ttcaaaaaat ttgtaaatgg
     4021 tgtagaccat tcttgacttc ttctctaaat aagagtactt tagaggtaga aaaacaaagg
     4081 gagagaatag aatgagaact tttatctatg ctgacaaatt ctttttagcg tcaggtgtca
     4141 aacatgcagg ctatcttgaa attaccgatg gactttttgg tgaatacacc aaagaactac
     4201 ctgagggaga agtcaacatc attgaccaaa gcggcaaatg gattgctcct ggtttggtag
     4261 atacacatat tcacggctat atgaatcatg atgtaatgga taatgatgca gaaggcatca
     4321 aagtgatgtc tgaaggtctg ttatcttgtg gagtgacttc atttttacca acaacattga
     4381 cgtcaagcaa agaacgttta aaagatgtag ctgagacaat cggcaaaatg tatcaggaag
     4441 caccaggagc taaaatcaaa ggaatctatt ttgaaggtcc tttcttcaca gaagaacaca
     4501 aaggggcaca aaacccaagt tatttcgggg atcctgattt ggatactttc catgaatggc
     4561 aagaagcttc aggtggttta atcaaaaaaa ttgctttagc accggaacgt caaggtgtag
     4621 aagagtttgt caaacaagtg actgaagaag gagtagttgt ttctctagga cacagtaatg
     4681 ctacactaga agaagcgcaa aaagctgttg aagcaggcgc aagtgtattt gtccatgctt
     4741 tcaatggtat gcgtggattg aatcatcgcg aaccaggtat ggtgggtgcg ttattatctt
     4801 tagaacatgt cttttctgaa ttgatttgtg atggccatca tgtacatcca aatgctgcgg
     4861 gaatcctaat ggaaaaagca ggacatgatc atgtcgcatt aattacagac tgtatgatgg
     4921 ccggtgggat gcctgatgga aattacaatt taggcgaatt tccagtagta gtaaaagaag
     4981 gaacagcacg tctagaatct ggcaacttag ctggaagtat attgaaattg aaagaagcaa
     5041 ttaaaaatgt tgtagactgg ggattagcaa caccagaaca agcagtcatg atggctagtc
     5101 tgattccagc aatcagctgt aaaatcgatg atacatgtgg aaaaatcgct caaggacgcg
     5161 atgcagactt tatcgtattg aaccctgata tgacattgga agcaacttat ctagatggcg
     5221 tggaacgttt ccacgcataa tataatttaa gttaacctta aaactaaact tcttaagttt
     5281 ggttttaagg ttttttttgt gttctagtaa aaatcacttt acataagttt caaaagtttc
     5341 tttaatttcc tattgttcaa taagtaacaa tgtaaagata aaattaaaaa tgttaatacg
     5401 tattagcaaa ataaaagctt tttttattgt gaataaatga acataattag gaggagaaag
     5461 atgaacttat ttgacatttt tcgatcgact ttgacagata tgtcgattgt cagtgcaatc
     5521 acttcaacag tttttattat attattaggg tttgtttgta gaaaaaaggg gattttttct
     5581 gaatctgttg gaaaaattct atcgaaagtc gtcttaacag tggctttgcc agcactggca
     5641 tttcgagctt tcatgcaaga tatcaataca gaattactga tgcaaggaat caatgtcttg
     5701 atttggggat tagttattta tattattctt attctagttt caaaaccttt tttcttaagc
     5761 tataaagggg ataagcaaac aacgttgcgt gtcctgacga tctttggttc tacgaccttt
     5821 ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta ttcctcaatt
     5881 ttcaatattg gctaccgaat ctttttgtat tcttatggat acataaaaat gagtggcttg
     5941 aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc caatcattat cgctactttt
     6001 gccggccttt tcatttggct gttccaaggg tatctgccac aagtgtcagt tacaggtgca
     6061 gaaggagaga taagccaagt agcgtttttg cgaattgacc agactgcacc gtggctattt
     6121 cagccgatgg attatttagc tggtttaagt tcaccattgg cgtggctagc aatcggcgca
     6181 acattaggtg aagtaagctt caaaaaagct gcatctgaaa aaacttcatg gtattacagt
     6241 ctagtcaaag tggtatttgt acctgcattg aatatcgtga ttctagcagt tttgacggca
     6301 acgaatattc taccagtcaa ttttgaagca ctggcaacaa tcgttatcat gatggctaca
     6361 ccagctgcaa cagtggcagc agcctatgct atcagttttg atagggaggc acttttagcc
     6421 tccaatgcat ccttactgtc aactattttt gctgtggtac tgacacctgt atggatcgtt
     6481 gtattacaaa tgattcatgg cataggttta ttttcataag aaatggagga agaaagtatg
     6541 agaatcgcat gttacggtgt tcgtccgaac gagatagatt tttttgaaaa attgaatatt
     6601 taccattatg atctcagtct ttatgaagaa ctgttgacac atgacaatat cgtgtcagca
     6661 aaggaccatg atgctgtatt gttgagggga aattgtatag cagatgagac caatcttgca
     6721 aaaatgcagg aatatgggat tcgttatgtt tttacacgga ctgtgggagt caatcatatc
     6781 gatctacaag cggcagctga ttttggtatg acagtagccc gtgtaccatc gtattcgcca
     6841 aatgcgatag cagagttatc tttgactttt gccatgatgc ttttgagaaa tactgcgtat
     6901 acgacgatcc gaacatcttt caaagatttt cgagtggatg agcaaatgtt tagtcgtgaa
     6961 atcagaaatt gcacagtagg gatcatagga acaggcagga tagggttgac ggaagctgtt
     7021 ttgtttaagg gactaggagc caaagtgatc ggctacgata ttcatcagac agaagctgct
     7081 aaaaaagtat tgacgttcca ctcgcttgaa gagttattag ccaaaagcga tattgtcagt
     7141 attcatgtac ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg atttttagct
     7201 caaatgaaaa aaggtagcat cttgattaat acagcccgtg gagagcttca agataatgaa
     7261 gctattttaa gagctttgaa aagcaatcat ttagctggtt ttgcaaccga tgtttttgca
     7321 aatgaaacag aggttttttt ccgatccttc aagccatggg agacgattcc agaccctgct
     7381 atccagcaat tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt aggttcaaat
     7441 acagatgagg cattatcaaa tatgatttcg acaagtttcg agaactttcg tgaaataata
     7501 gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt aagataagaa
     7561 aacatggata ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt tgttacttct
     7621 gtatacttgg actattttgt atgggataat tcgatgactc aacagtaatc tgtgagcttt
     7681 ttccgcccat tctttgtcaa ttcataacag gttagcggcc ggccgatttc caaatatttg
     7741 attctttcgt tcaatatatc ttgttctact aaaaaattca aatatttctt gacggatata
     7801 cgtgacagtg agcttttttg agccagttct tttgttgaaa aaatagtatc ttcttttaga
     7861 ataaatgcac ttattttttt taaagtcagt tttgacagac ctttaggcaa atcgatgaat
     7921 gatggtattt cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa tttctgatcg
     7981 gctttttcag tcgtttggat tgtctgagag aaagcaagat acttttcgat cgccagctga
     8041 aaacgttgca aagtgaacgg tttgatcaaa taatctaagg caccgtagcg gatcacattc
     8101 tttatggttt cgatatcatc tgcagcagtg atcaaaatca ctgggatatc atttccttgc
     8161 tgtctaagcc aagataaaaa atggatacca ctttcgccag gcatgtatac atcaagcagc
     8221 agcagatctg cgtttttttg tctgaaatat ttttttcctt cttctacatt tctgaaactt
     8281 tcgattacgt tcatattagg gatgctttcc acaaaagtac gattcaattc tgcaaccatt
     8341 ggatcatctt cgattatcag gacatttgtc atcattttga tcttcctttt ttggtaattc
     8401 tagatagacg attgtaccgc catttgtttg tttggaaaat tctaaaaatc caccgtggcg
     8461 tttgacgatt gaatggatga gattcaatcc aaacccacga ttgattccct tcgtcgaaaa
     8521 acctagcgta aatatttttt ctgagatttc ttctgatatc ccgacaccgt catcgcagac
     8581 ggtcacgatg aatacttcag cttctgtgtc atagtttaaa tgaatccgga cctgtccgtt
     8641 tctttcatgt ttttgtagca cggcttcttt tgcattatca agtaagttcc ctaacacttg
     8701 caaaagtgaa tggatggagt catccatctc cattttaggg ataaaagaat cgctttctaa
     8761 cagcatttca acagattgtt ctttggcttc gtttattttg cctaataaaa aaccggcaat
     8821 tgcaggtact ttgatcagac ttgagacttg gccaatctct tttttataat tttgatttag
     8881 ctgctggata aaatgggtga cttcttcata cttcttcaaa tcaatcaaac cagaaatgac
     8941 atgaagttta ttcatgaatt catgggtttg agctctcatg gagtttatat attgtttcgt
     9001 ccccccaagc tcctccatta atttcattac ttctgatcga tttcgtaaag ttgcgactgc
     9061 tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta gaaatagttt ctatcccatt
     9121 taaatacatg gattgagtcc gaacggtcgt ttgatgctca atcgtctctt taaacaaaac
     9181 gtcatataga ttatcactga tttttttcct acttcgaaat ccaatagctg ctctgtttgg
     9241 tagaaaatgt tttttgcagg tgtattgatc aagaggatag ttttatcaga agagatggct
     9301 aagattcctt catctacttc attttcgata atcgtttttt cacttaactg ttctgctatt
     9361 tcttgtggtt caagtccaag cagtactttt ttcatttttt gggcaagaaa aacggcgcct
     9421 atgatgccta cgctaagtcc aataatcaat gaaaaaacaa tcattttttg tccatctttg
     9481 acttgattgt caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat aatctcttga
     9541 tcataataaa tagggacgaa atagcggaag ccgaacccta acattccttc tttttcagag
     9601 aaaaattctt gtcctccaat cacttttttc acatcttcat gacttgagaa aggaagaccg
     9661 atgtaacttg ggtctgcgtg agaatatcga atgccttcag ggtccatgac aacgataaaa
     9721 tctaaattcg atgcaatcat gatttcattt gttattcctt gaatcgcatg tgtcgggtca
     9781 ccagtctgca gctttttttg gatgagttta tcattggcaa cgattttagc aatgctagag
     9841 attttatctg acacactaga tcggttcatc gaaagaataa aattcctgat caatatggca
     9901 gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt gttctggagc
     9961 tttaatggtt ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat ttattgtgaa
    10021 aattttaaca tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa ccttcctata
    10081 aaaaacacca ctgagtcggt aaaagaagct atacagaatt ttcaaacatt ggtatgatag
    10141 acgtagaaat ggaaggaggc gtgtatatga tagggggaat cgcacttgag acgatttatt
    10201 ggtatacatt gctgatttgt gcaggattgg cagtcctatt gattattttt ggagatgtat
    10261 tcgattttga tggcccgctt gatccgatgc ttattatccc ttggttaact ttcacaagct
    10321 tatttggttt tctaggtgag cgtttaacag atcaatcatc tttattttta tttattgcta
    10381 gtggaggcat atcgatagtg ttggtatttt tgcttaattt ctatatattg atgccgatga
    10441 aacatgccga atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa gtagctgttg
    10501 tgatcacacc gatccctgta cgaggtatgg gagaaattca gttgaaaagt gttaccggtt
    10561 ccattagtcg tccagcttgt ttttacagcc cccaagaaca gacagctgag cggggaacga
    10621 aagtattgat cattgagatc aaggaaagaa tctgttatgt catgccttat gaaagcatga
    10681 taaaagctta aaggagcgtg tgttaaatgg aaataccagt tgtttttatc gcgatcgcaa
    10741 tcgcagcttt gattttactg atgttgctga tcgtctttgt agcgaagtac cagacagcaa
    10801 aaccggatga agcgttgatc atcagtggaa gttatttagg aagcaaaaat gtgcacattg
    10861 atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc ttgccagttt
    10921 ttcaacgttc gaatcggatc agtctattat cgagtaaatt ggacgtctcc acccctgaag
    10981 tgtatacgga acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc aaaatcggtt
    11041 catctgtaga agagattgct acagcagcag aacagttttt aggcaagaca agagaagaac
    11101 tggaaaatga agcaagagaa gtattagaag gacacttgcg ctcgattctt ggttctatga
    11161 ctgtagaaga aatctatcaa aatcgcgata agttcagtca aagcgtacaa gaagtcgctt
    11221 ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa gaggtcagag
    11281 acaaaaacgg ttatcttgac tctttaggga aaccaagaat cgcccaagtc aaaagagatg
    11341 cagatatagc agaagcggaa gctctaaaag aaacgcgtat caaaaaagct caagcagaaa
    11401 aagaatcaca agcagctgaa ttacagcgac aaacagagat cgcagaatca ttaaaagaaa
    11461 aagaactgaa attagcaacc tacaaacaag aacaagatgt tgctaaagca aaagcagacc
    11521 aagcctacaa tttagaaagt gcccgagcac aacaacaagt aatcgaacag gaaatgcaga
    11581 ttaaaatcgt agaacgtcaa aaacaaatcg aactggaaga aaaagaaatc acacgtcgtg
    11641 aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac gctaaagaac
    11701 aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa cgattccgtg
    11761 tggaagcact ggcagaagcg gaagccaata agacacgttt agctggacaa gcagaagccg
    11821 aagcagcact agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc gccaatgcct
    11881 tcaaggaata tggcgaagca gctgtcttga gtatggttat cgatatgcta ccgcaattga
    11941 tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc gatacaggaa
    12001 gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca aatttgcttt
    12061 cttctactca agaaacacta aaagcgacaa caggacttga cttgaaggaa atcgtagaga
    12121 atttgtcgac acgtgggact actagccaag taaatatgta tgaagcgcca aaagaggcag
    12181 aacaaaactc gatgaaggaa gaataaagta gtacacttca ccgttcaatc aaaaatggag
    12241 gaagactttt taaagttctt ctctccattt ttttatttta ttgcaaaaga aaattagtcc
    12301 tcgctatttt tcttttgttt ttggatctta gcttctatct cttgtaaatt tgttcttagc
    12361 aatcgttctt tcttttggag ttcgaccaaa tcttctgata gttcttcctt cttttctgtt
    12421 tcaggaggac gaagaaagaa attgataccg attgcaatga atgtccctat aaaagtatct
    12481 agtacacgtt ggatagcata caaagcggat tcgccttgag ggatgctcaa cgcaatcagc
    12541 aacaacgtag ctattgcgga aatgataccg gaattattgt taataccatc tgacacgacg
    12601 atcacaataa taactagaat aggaagcaag atcaattcta ccaaaaaatc attgtgaaaa
    12661 tagcttttga caaaaaaata aatgatagct aatgtaccac ctaatgtatt gccgatgatc
    12721 ctagactttc caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa aacagcagat
    12781 aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat acataaaaaa
    12841 acagctaagg cacttttgat cgtacgcata cctagtcgaa aacggccaaa ttgcatagag
    12901 attctccttt ttggtctttt tactatcata cctaaacttt tgtctgatag ctagtttttc
    12961 attattcaag tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg aatgcgaaca
    13021 gtcaaaacag tgatcagtgt cttcttttcg atggtcacag cgggtagctt atcgctgttg
    13081 tattggccag ctgcagggat cattgcatta ttaagtgtag gaaataccaa gcgctctaca
    13141 ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag ctacggcaac agcactgttc
    13201 agtttttttg ctgttgggta tactattttt ggcttcagtc tttttttatt cctgttcatc
    13261 ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag taaattcggt tttggtcact
    13321 cattatttag tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc gggattaatg
    13381 gcgattggat tagtatttgc tttgcttgca aatgtttaca tgccagatcg tacgaagcag
    13441 ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa atattttgag acaaatggca
    13501 gaatttcttt tatcagaaaa taaagaagat gtgcagatca aatgcgaaca tttactgaca
    13561 ttcattcgcg aaagccaaga agatgccaga gaacatcaag aaaactactg gctcagacag
    13621 ccgctatact acgaaactta cttctctatg agaagagcac aggccaatgt gattaaagac
    13681 atgctggaaa atctagagag aatccagcaa ccagcttatt atggaaaaca tatctatggt
    13741 ttattgattt atactgcaga aacattttca gagtcaaatg atggcagaca aattttaaca
    13801 cgtattgaag aagtttatgt attgtatcgg caaatgcctc tgccgactag tcgccctgaa
    13861 ttcgaggatc gagcagaatt gttccagttt ttgcagtctt ttaaaagttt tattgaaatc
    13921 aaggcagaat tctcccaaca aaaaatccaa aaatagaggt tgtgaattca gctaatattc
    13981 aggtcaaaaa gctgatttca caacctcttc tttaaacggt gttctcctgt caactcctcg
    14041 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    14101 ctcggagctg aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa agctttcctc
    14161 tgcggtaata agtccagcca aacgaaaaat aggaggagct atttttgttt ggctgttctt
    14221 attacttgat gcagaacagc tttttcacaa ccttttaaaa ttacatattt ttccatagaa
    14281 cttgcatttg atccaagatt tcgatgacat cttttgtcaa gaagagagag cgattgggca
    14341 agtcaccttc aatcatgtgt accatattct ttatttcgta attcattgct tcagatgtat
    14401 tcccgctttc gatgaattcc tttgtaccat ctgtaaataa gatctccgca cgatcagcac
    14461 gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc aacgataccg acttttggca
    14521 tttttgcctg aaaagtcaag ctgacagcag caagttcatt ttctttgtta cgcaagatag
    14581 tgacggattg ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca actacttcag
    14641 gttgagagct caaaaagaaa cgagcgaaag atacagcata cgtaccgata tccaacagag
    14701 ctcctccagc taattctgga ttgaagaacc ggtttttagg gtctggttct ttgtaactgc
    14761 cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc tgtatccatt attgagcgta
    14821 actgctgata aagtggcatg ttgaagattg tcatcgcttc tgctaagatc aaattctttt
    14881 cttctgctaa gcgcatagcg tcagccagtt cttttttatt catcgtgatg gctttttcac
    14941 atagaacgtg tttgcctgct tccaatgctg ccaagatatg gtcgatatgc tgtctatttg
    15001 gtacagcaat gtacacgatg tcgatttcag catcgtctaa catttcttga tagcttccgt
    15061 aagcttttgg aatattgtaa cggtgagcga agttttccgc tttttctgaa gttctggaag
    15121 caactgctgt tagttcgctc gtctcttgat tgaagctttc ggcaaactca tgtgcgatgg
    15181 taccaagacc aacgattccc cattgatatt ttttcatctg aaaacgctcc ttcatagttg
    15241 gattgagttt cttgtctgtc tattattata acaaaaatca cgaagaataa agaggagagg
    15301 acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg atcaaaactt
    15361 aagtagaata gttaaaagga ggggataaac gtgcatcaag tatatattga tcttattatg
    15421 agtgtattat tagaacagtt tgaaactgaa aaaaattttt tagatgaata cttgctgatc
    15481 agtccagaac aatggcaaaa atggaaagat ggcgagaaaa atctgccatc tgaagcgatg
    15541 cagaaagtaa aaaatctttt cactgattat gaatggatgc taacgcaaaa gattttgcgt
    15601 caaacgatta tttttcccga aaaacgaaac gttgccgttg cagagtacaa gcaaatcaaa
    15661 acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct gatccctttg
    15721 aaaaataatc agcaggctgg tagatacctt gacttgaaag taagtattca atatgatgaa
    15781 tggggctatg atgatatttt gaattttcgt ctgccagcag atgttcaaaa acagatcgaa
    15841 ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc tggaagaaac ttacgttcat
    15901 gaggcgaatg gggaacgtga agaacaacga aaggattttt caagtgaata aatttttagg
    15961 atttggagca gttgtgatta tgatcgcagc aatgggctat gttattttta gcgatgatga
    16021 ttcgcatgtt caagccggca atcaagcagt cgaacgaaca agcaccaaaa caactgctag
    16081 taaagaaaag aacagtcaaa caaccagcag caaggtaaag gacaaagaaa caaaagatcg
    16141 tacgtccaaa caattgccag atgttaaaac agacgattgg aagctagttt tggttggtcc
    16201 agatcacaaa ttagaaaaag aaattgatga gtctactcaa ttggcatcat taagtaacgg
    16261 atacttgatc gacaaacgca ttgctccaga atatgaagca tttgccaagg ctgctgaagc
    16321 agctggattt ccattagtga tggtctcagc ttaccggtct gtatcatccc aacaacaagt
    16381 tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag aggaggcgac
    16441 aaagattact aaagaaacga ttacggagcc aggatacagc gaacaccaca caggtctggc
    16501 agtcgatgtc gtcgatcaaa actggtacaa cagttatcct tctactgtat tggatgcaag
    16561 ttacggagat cagcctggag ctaaatggat cgcagataat gctgcgcagt atggatttat
    16621 cgttcgttat ccaaaaggac acgaagatat aactaagatc acatatgaac cttggcactt
    16681 acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa caccaattga cgctagaaga
    16741 atatctgaat gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa agtggaaaaa
    16801 gcaaaagcgc aagaatatcc gttggccagc agtggttgcc ggtgtagcag tgatactctt
    16861 agcctttgtt ttttccttga agaatctgtc ttctcctttt actgataatc agcaagaaaa
    16921 tgaagcgcaa tcccatcagc aatttattga acggcttgtt ccgcatgccc aagaactaca
    16981 agatggttat ggagtgcttc ctagtatcat tttaggacaa gctatattag aatcaaattg
    17041 gggaaaaagt accttggcta gcaaatacaa taatcttttc gggatcaagg cttatgggga
    17101 tcagaagaaa gtaagcctgg aaacaaagga atttgttaac gaagaatgga taactatcca
    17161 aggagacttc aaagtgtatg attcatggga acagtccatg gatgaccata cacaactgtt
    17221 cgtccaagga gtagattgga atcctgcgct gtatgaaaaa gtgattacgg ccaccaacta
    17281 tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg acagaccccg gttatgcgca
    17341 aaaaatcatc caggtcatcg agacgtatca attgaatcag tatgaccgga gatagcaaag
    17401 gagaagtggc tgatggcaga acaatttacg ccagaaatca tgacggaatt gagaaaagat
    17461 atcgtaagag taccggaaat cattttagaa gcaagcggga tcaagatatt tggaaaattg
    17521 atccgttcga tcatctttac tacggatatt gcgatcatac gtaatacaaa tgcgaatgca
    17581 gtaattgctg tgtatccttt tacacctcat ccagcgatta ctaaaagcat tattgaggca
    17641 gctgacattc cagttttctc cggtgtaggt ggtggtttga cacaaggtgc tcgttcttcc
    17701 tacatgagca tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa cggtcctacg
    17761 cctttagaga cagtaaaaga agtttgtcga gtagttgata ttccagttat cagtacggta
    17821 acgtcgaaat atacagaaat agatgaaaag ttagcagctg gtatttcgat tatcaatatc
    17881 agtgcaggta aagaaactgc agaagcagta cgccattttc gtaaaaaata tcctgaactt
    17941 cctatcattg cgacaggtgg tccaacggaa gaaagtattg ctgagaccat tcaagcagga
    18001 gcaaatgcca tcacatatac gccaccaagt agcggagagc tttttagtcg taaaatggaa
    18061 aaatatcggg aaactgagaa aaagaatcat gaataacagg tgagaatcaa aagacgagga
    18121 agagcagata gtggctcttc ctcgtctttt gtactccagt actatttgtt cagaattcaa
    18181 aaaaaagaga aatttttttg tttttttacc tgtaatgtac ataagaagag ttaaaacctt
    18241 ttgttattat tagcattcta acaaaaaaat gtcttgattt attgaagcgc tttctataga
    18301 atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc taaattgtcg
    18361 gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg cttgtgcatc
    18421 gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg ttttttaggg
    18481 aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg gcagttgtct
    18541 gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag tgtttttagc
    18601 gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg ttcatcctag
    18661 gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt ttaataaata
    18721 tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg tgcagagaat
    18781 gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct tgacggcttg
    18841 tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt cgcaagtccg
    18901 acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc atgtgacgat
    18961 cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac tagcagcatc
    19021 agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca tcaatcagat
    19081 tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg cagatgaatc
    19141 ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg gggtgaattc
    19201 gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg aaccagcttc
    19261 aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag aattaggaat
    19321 ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa taccacaagt
    19381 tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg gatttgaaaa
    19441 tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca aaggggccca
    19501 tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg cggttaggga
    19561 tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat tgtcaggggc
    19621 tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag atggagaatc
    19681 tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag cccataaaga
    19741 agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg aaattttaaa
    19801 aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc gtgcagatga
    19861 tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg acaacggcga
    19921 agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca atcttttagt
    19981 tgaaaaagtc atttatgaag aagtaacagc agaagaagca gcagagagtc tttatgagtt
    20041 tgcgtcaaac attctaggat agcagcagga ggaaggcatg aaaaaagaaa aactaaaacg
    20101 gttcttggat caagacaatg tggtcggtta cgtatttgct tctccattca tcctaggatt
    20161 tctattgata acagtgatac cgatgatctt gtcactttat tattcgttca ccgattatcg
    20221 tttgggaaat ccagtctcat ggatcggctt ggataattat ataaagatgc tgagtgatgc
    20281 ccgttttttg aactccatcc gagtcacttt tctctatgta tttgcatctg tacctgtgaa
    20341 actgatcttt ggtctattga ttgcttatct tttaacgagg aatatcaaag gaacgacttt
    20401 ctttcgttct ttgtattatg tgcctagttt gatcggtggc agtatttcta tcgctctagt
    20461 ttggcgggag ttgttttcta aaaacgggtt agtgaatatt ttcctggaac gattaggcgc
    20521 acctgcgctt tcctggttta gcgatcaaaa actggtcatg atccctcttg ttttgatgtc
    20581 tgcttggcaa ttcggttcgg caatgatcat ctttgctgca ggtatcaaag agatcccagg
    20641 ctcgtattat gaagcggcag agatcgatgg agcgaacaaa tggcagacgt tttacaagat
    20701 cacgttacct cttctttcgc cgatcatttt gtataacttg gttatgcaga caatcgcggc
    20761 gtttatgact tttacgcagg catttatcat tacccaaggt ggtccgaatg atgccacgaa
    20821 cttttatgct ctttatgtgt ataaccaagc tttttctttt aattctatgg ggtatgctag
    20881 tgctatttcg tggatcatgc tgttgattat gggagctgtc acctttctga ttttcaaatc
    20941 atcaaaaaaa tgggtcgttg tgtgaatgag aggaggaaga aatgaataat acagtaacga
    21001 aatggtttca atacggtttt attttgctat taggtctagt catgatttat ccggtcttat
    21061 ggatgatcgc aggttctttt aaaacggatc aagagattat gagtggttcg ctgaatttga
    21121 ttccagagac ttttcaatgg gaaaattacg caaaaggatg ggcggggttt gctggaattt
    21181 cattttttac tttctttagg aattcgtttg tcatctctgc agtttcgacg atagggacag
    21241 ttctgagctc gacttgtatt gcttacgcat tatcgagaat cgatttcaaa ggaaaaagat
    21301 tctggttcgt cgtcatgtta gtaacgatga tgattcctgc acaagtgatt ttgattccgc
    21361 agtttattat ttacaatcgg ctgaatttgg ttgggacgta tgtgccgctg attttaccac
    21421 atttttttgg acaagcattt ttcatttacc agatcatgca attcatggtc agcatcccca
    21481 aagagttaga tgaatcagcg attattgacg gatgcagcaa gtatagtgtc tttaccaaaa
    21541 ttatttttcc tttgctgaaa ccttcgatca tcacgacgat catcatccag ttttattgga
    21601 aatgggacga cttcatggga ccattgattt atttaaacaa gccgagatct tacacggtat
    21661 cgattgccat caaattattt gcagacgctg gatctagtac gacttacgcg agtatgtttg
    21721 cgatgtctac gttgtcgctg ctaccggttt ttctgatttt cttattcttc aataaatatc
    21781 ttgtccaggg gatcagtaca agcggtctga agggctgaat atgaaaaagc caatagaaga
    21841 gatgtttctt tacaagctca actactatgt ctgtcggatt tttttaacca gtcttatcag
    21901 taatgctctt ttcgctttag ttctaggatg tttgttcact ttaggattag tgctgtctgc
    21961 tcgtgttgtc tatctgattt attttttgat gatgtatcct tcttattatc tatctgtcca
    22021 ttacttggca gaaaaaaagg tatgttggag gaaaggaaaa atcaaacgga tgtgtttcat
    22081 cattacgttg tttcagctgt ttgtcattat ttttgcagtt gattggtatt tattagtggg
    22141 aaagattgga gcttggtatc tgattccttt tctattgtgt ctgttgcttt ttgtatccca
    22201 tagttttttt agtacgctaa ttgatatctg caaagaagat aaagcagata gtcttgttta
    22261 tttgatcaaa aggaatatct ggctgttgtc aaaaaacgtc aaggggtttc tgtggtattt
    22321 atgccttttg tatggactgt tctggtgtat acagcatgat tttttactca ctgcattact
    22381 gtatccaggg ctgccatttg tcacagtatt ttgcttaaaa gaaaaggaga atgactttaa
    22441 atgaagaaaa aatatgtaca agtcggactt ggtgggagag cgcgtttttt ttatgaggca
    22501 attgcctcta cgtatcatca aacttgtgaa ctagtcggtt tttgtgatgt atctcaaaca
    22561 aggatggatt atgcaaatcg gatacttgtg aacaagtatg gacagaaaaa agtaaagaca
    22621 tgtatgccat cacaatttga gcaactactg gatgacgtac ggccggctga agtcattatt
    22681 acgtcagtag atcgaacgca tcatgattat atcattcgtg caatgaaaaa aggatatgat
    22741 gttatttgcg aaaaacctct gactatcgat gaggaaaaag ctcaagaaat aataacggtc
    22801 caaaaacaga cgggaagaag cctgcgagtc acttttaatt atcgttatgc accgtatcac
    22861 acgaagataa aggaactgat agaaagtgat gtgattggtg atgtgttttc tatccacttc
    22921 gaatggctgt taaatacgca acacggggca gattatttca gaagatggca tcgaaataag
    22981 cacaatagcg gcgggcttct tgttcataaa tctacccatc attttgatct agtgaatttt
    23041 tggttgaaca gccgtcccca aaaggtattt gcattcggtg gattaaactt ttatgggatg
    23101 cataatgcgg agaaacgcgg agtagaaata ttttacagtc gatgctatgg aagcgaacta
    23161 gctaaagctg atccatttgc tatagacctg tcagccaatg aggaattgcg agaaatgtat
    23221 ttagaagcag aaaaagacag tggatatatt cgtgaccgca gtgtattttc cgatgatatc
    23281 tctattgaag atacactagg cgtaacggtc aaatatgaaa ctggtgcgat tttgacgtat
    23341 tcgctaaacg cgtatatgcc gtgggaagga ttcaatgttt ctttcaatgg cagcaaagga
    23401 cggatcgaaa tgaaagtgat cgaaaagtct tacatcaatg caggtggaga taaaaaggat
    23461 gaaggagcag ccgttcttca gtccatccat gtctatccga tgttcggtga gccatatgag
    23521 gtaatcgtcg aacaagaagc gggcggacat ggcggaggag atccgcgttt attagatgat
    23581 ctatttggtg aagaaaaaga agaagatccg tggaatcggg cagcaactca cgtagatggt
    23641 attttgtcga ttttgacagg aatcgcagca aatcattcta ttgcttcagg aaaagcagta
    23701 gtgattgacg agcttgtcag cttaaaagaa taagaataga tttacggaaa ttacttcccg
    23761 tagtttataa aatcttgcct tattgccgat aggctgtctt ttgaacacta tttattcgga
    23821 aaaaggggct atgacatgct ttttgtcatg ccttcttttt atgtgggctg cttttttaga
    23881 taggagaaaa taaaaataac tatttaaacc cgaacaaaaa ggaataatgc attaaaaatc
    23941 attagatttt acaaaaaaat aagtaaacca cgttgtcagt ttcagaaagt agtgttagac
    24001 tgttttcaat atttgaaaca acatttttta ttcaaatatg gcaaactgcc ttcacgtgca
    24061 aaacagttgc aagaaaatga atttacacta ttattagttt tacataccta cttatatatc
    24121 gtcataatat ggatgacagt ttctagccag taccgtaaat gctggactat aagtaaaaga
    24181 ttggctattt cacccttctt ttcttatgat aaagtggagg gtgtttttct ttaacagaaa
    24241 catagtaggt ttttgcacaa aagcggttat ttcggtatta ataaatgata aaggagcaaa
    24301 taaagtgaaa gaattagttg aacgtattca aaaagacgga cgtatattag gcgagggcgt
    24361 attaaaagtc gacagcttcg tcactcacca agtagaccct gtattgatgg aacaaatggg
    24421 aaaacggttt gcagaagtat ttaaagaaca aggaatcacg aaagttgtga ccattgaagc
    24481 ttctgggatc gcaccagctt tatttgctgc aagagaatta gatgttccaa tgatcttcgc
    24541 tagaaaagca aaaagtttga cgatggatga agaattattg acagcttctg tttattcttt
    24601 tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa tttttatctt cagaagacaa
    24661 agtattgatt atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt
    24721 atgccagcaa gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa agtctttcca
    24781 agatggacgt cagttacttg aagaaatggg attgagagtc gtttctcttg cgcgtattgc
    24841 ttccttgacg aatggacaag ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa
    24901 acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat gtatgcagga
    24961 gcagtagcgg tgcctctttt gatcggtaca ggactaggct ttaatcaaga acagatgact
    25021 tatttgattt caattgatat ctttatgtgt ggattagcga cattattgca attgacagtt
    25081 aaccgatttt tcggaattgg cttgcctgtc gttttaggat gtgcgatcca agcagtagct
    25141 ccactgatct tgatcggaac caatgaagga gtaggagcta tctacggatc tattatcgct
    25201 tctgggatct ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa actattccct
    25261 cctatagtaa caggaacggt tatcaccgtt attggtttga ctttgattcc tgtagcaatc
    25321 gagaaaatgg gtggaggtaa tgcaacagca gctgattttg gtgacaaaac aaatctgcta
    25381 cttgcatttg tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt
    25441 cgttcaatcg cggtattgat tggtttagtc ggaggaagca tcttagctgc tttcatggga
    25501 atgattgatc tgagcgcgat tggagatgcg ccgttgttcc atgttccaca acccttttat
    25561 tttggtaaac caactttcga cgtatggtcg attttactaa tgatcatcat ttccatggtc
    25621 agcatggtag aatcaacagg tgtatatttt gcacttggtg acattactgg gaaaaatgtt
    25681 ggagaagaag aattgaaaaa gggatatcgt gcagaaggac ttgccgttat cttaggcggt
    25741 attttcaata cattccctta tactggcttt tctcaaaatg tcggattggt tcaactttcg
    25801 ggaatcaaaa caagaaaacc catctatttc tctgcattct tcttgattgt tttaggatta
    25861 ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc cggtacttgg tggaggaatg
    25921 ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta tgctgttgga agttgatttc
    25981 agcaatgata aaaatctatt gattgttgct gtttctgttg gatttgggct gggtttcaat
    26041 gttacgccga cattattcag tcaaatgccg caaacattgc aaatgttcac tggaaatggc
    26101 atcgtaatga gtagcattac ggctatcata ttgaatttga tctttaatgg attgaaaaaa
    26161 gatgaagaaa tattggaaga agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa
    26221 tcagcgtttt gacctgattg ttagagaaac aaacggaaaa atagttcctc gtattttgcc
    26281 gtttgtttga gctaataccg agggtcagct gattgaacac cgttgaagga agaggtcgtg
    26341 agcctgccgt tcatctccga gtcacaagga acaatttcaa aaaaaagctt ctcatgtttg
    26401 ttgaatattg ttccttgtga ccgaggagtt ggcaggagaa caccgtttat ccataaaaag
    26461 gaatgtgacc taacttttgt cacattcctt ttttttatct ttaataaaca cgaacaataa
    26521 aatgtttttc tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa
    26581 tatgaatgta gaaaaactgg gaggaaaaac aatgacagca aaaatctctg tgattatggg
    26641 gagtacttca gattgggaaa caatgaaaga agcatgtcaa atattggatg aattcggtgt
    26701 gtcttatgaa aaaaaggtcg tttctgctca tcgaacccca gatttgatgt ttgaatatgc
    26761 acaaaacgct cgaaaaaaag gaatcaaaat cattattgca ggagcagggg gagccgcgca
    26821 tctgccgggt atggtcgcat caaaaacaac tttgccggtt atcggtgtac cggtccaatc
    26881 acgaacatta aatggtttgg attcgcttct ttcgattgtc caaatgcctg gaggagtacc
    26941 tgtagctact acagcaatcg gaaaagcggg agcaattaat gctggactac ttgcgataca
    27001 gatgctttcc atgtatgatt tagaattgga ggctaaattg gctgaaagac gttcaatgtt
    27061 ggctaaaact gttatagaaa gtagtgatca acttggttaa acctttgttg cctggttcaa
    27121 cgatcgggat cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa
    27181 tgggctttaa agtaggtgtg ctggatcctg tgagcgattg tccagccgca caagttgcag
    27241 attggcacat tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt gccagaagaa
    27301 cagatgtcat tacctatgaa ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac
    27361 ccatgtcatt tattcctcaa gggacagact tgcttgcgat tacgcaagac cgcctgttag
    27421 aaaaatcttt tttggaaacg aataacatcg tgattgctcc ttatgcaacg atcgtcagtc
    27481 cgacggatat ccaagatgcg attgacggga tcggctatcc ttgtgttttg aaaaccactc
    27541 gaggcggcta tgatggcaag gggcagtatg ttttgaaaag tcgggcggat ctagctcctg
    27601 ccatggacct tttaagagaa ggaacctgtg ttttagaagc atggatcccg tttgaaaaag
    27661 agatttcgat catggtggca ggaaatggtc aaggagattt cacgactttt ccggtcgtgg
    27721 aaaacatcca ccacaacaat attcttcatg aaacgattgc tccagccgcg attgatcagg
    27781 atgtgatcga ggaggctgag cgtattgccc gtgtgattgc agaagcagtg agcctttcag
    27841 gtgttttagg ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg aatgaattag
    27901 ctcctcgtcc tcacaattct ggacattatt ctattgaagc ttgttctatg agtcagttcg
    27961 atgcccatat tcgaggtatt tgtggttggc cattaggaga aaaagtacgt ttgataagca
    28021 atgcagtaat ggtcaatgtt ttgggagaac aactgctgga aagctatcgc ttgattgaga
    28081 ataagccaga ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga
    28141 tgggacatat cactatcttg acagaagaca tccagcaaac gctggaagaa atcaaacaaa
    28201 cgaatatttg ggattagagg agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg
    28261 ggaatatctg gacggaagag aacaaatacc gtgcgtggct cgaagtcgag attttagcag
    28321 atgaagcttg ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc
    28381 atgcatcatt tgatatcgat cggatcttgg aaatcgaaaa attaacacgc catgatgtcg
    28441 ttgcgtttac acgagcggtt tctgaaacat tgggagaaga aagaaaatgg gtccattatg
    28501 gtctaacaag tacggatgtc gtagatactg cttatggcta tctcatcaag caagcaaatg
    28561 atattttacg ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac
    28621 acaaatatac agtgatgatg ggtcggacac acggtgtcca tgctgaacca actacttttg
    28681 gattgaaact tgccacatgg tattcagaaa tgaaacggaa catcgaacga tttgaacatg
    28741 cggcaaaagg agtagaagca ggaaaaatct caggagcagt gggaacgttc gcaaatatcc
    28801 cgccatttat tgaagagtat gtatgtaaac aattggggat ccgtccacaa gaaatctcta
    28861 ctcaagttct ccctcgggat ctacatgctg aatatttctc agcgatggcg cttattgcta
    28921 caagtattga acgttttgca acagagatac gcggattgca aaaatccgaa acacgtgaag
    28981 tagaagaatt ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc
    29041 cgatcggttc agaaaatatg gctggtctag ccagagtggt gcgcggacac atggtcactg
    29101 cctatgaaaa tgtcacatta tggcatgaaa gagatatttc tcattcttca gcagaacgaa
    29161 tcatcttgcc tgataccacg attttattga attacatgtt aaatcgtttt ggcaatatcg
    29221 ttaaaaatct aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca acatttggtt
    29281 tgatttatag ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca agagaagaag
    29341 cttatgatct tgttcagcca aaaactgcgt acgcttggga caaccaagtg cagttccgcc
    29401 cgctgttaga agaagatcca accatcacat cggtattatc taaagaagaa atagacgatg
    29461 cctttgatta tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg
    29521 aataaagaag agtgagttgc aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa
    29581 gaagcagatc cgttagcggt tctgcttctt ttagttgtgc gccctgcatg ggtgaaaact
    29641 tggtggtgaa agtccactac aggcttggca gtaggaactg ttagcgaaag acaaggcggc
    29701 aatcgtgagg ttgtggctga aggaagtcgg acgcaaactc ctgaactgac gaacagaaac
    29761 tagatacaag gctgaattag gtcggataag tcttcatcac aagacaaagt ccaatactgc
    29821 ccgaaaccta tacagtaaat ctagcagtta catgggagga aagttttcac tcttaccggg
    29881 ggagatctca tgagggtgtt gtgggcgttg aatgaaaaca aaccagcaca acgatcatgg
    29941 cgacatgatg gtgaatcatg agaagtcagc cgaggtcata gtagtaacga cactgaccgt
    30001 tacgaaggac tgaacaataa gaatcttgaa taatttagga ggtgtgagaa gatgcgacga
    30061 ccgcagaaaa caagaaaact tggctatcat caaagagcta gtgtggaaca cgaagggtat
    30121 gatgaagtgc gcagcacttc ttgcggtgaa cagaggaaac aagatggtgt agaaaatctg
    30181 tttgggagaa ttctagatcg gaataatctc aatcaagcgt atcttcaagt agtcagaaat
    30241 aaaggggcgg ctggtgcaga tgggatgacc gtcgatcaac tgcttcctta cttgaaagag
    30301 caccgcgagg aattactatc caatttacgc caagggaact ataaacctca gcctgttttg
    30361 cgagtagaaa taccgaaacc aaacggcggt gtaagaaaac taggaattcc cacggttatt
    30421 gatcgtatgc ttcaacaggc aatcaatcaa gtgttacaac cgctatttga accagaattc
    30481 tcagataata gttatgggtt tcgcccaaat cgcagcgcac atgacgcagt gaagaaagca
    30541 aaatcttatt atgaacaagg ttaccgatat gtggtggaca ttgatatgaa agcctacttt
    30601 gacaccgtaa atcacgataa gctgatgtat ttcattgagc ggaaagtaaa ggacaaacaa
    30661 gtcctaaaac tgatccgtca atacttaatg agtggtgtaa tggaaaatgg actcatcaca
    30721 acaactgaag aaggaacgcc ccaaggaggc aatctctctc ccctgttaag taacatctac
    30781 ctgaacgaat ttgatcaagt gctagaaaat ctaggacata aatttgttcg atacgccgat
    30841 gactgcaata tctatgttag aagcaaacga gcaggacagc gtgtgatgaa gaaatccatt
    30901 caatttctcg aagaacaact aaagttgacg gtaaatcgag aaaagagtgc ggtaggaagt
    30961 ccattgaaac gaaagtttct tggattctgc attctgccaa caaagaaagg tattaaaatt
    31021 cgccctcacg caaaagcaaa acagcgtgtg aaaagcaagc tcaaacaact cactaaaaga
    31081 aaccgtggaa gaagtatcca acttatttta aaagaaatca agcaactaat gactggttgg
    31141 atcaactact atggaattgg tgagatgaaa gaattcatga gggagttaaa cggttggttg
    31201 aagcgaagaa tcagacaata tatttggaaa caatggaaga atccaagaac caaaagaaaa
    31261 aatctgattc gtttagggat cgataaagcg aaagcgtatg aatggtcaaa tacaagaaaa
    31321 ggtatttggt cagtctccaa aagtcatatt cttcatcgat cattaacaga caaagaactg
    31381 gcccgccaag gatatgagga catctccttg aagtaccagt tcgtacactc aacttattga
    31441 accgccgtat acgggtccgt acgtacggtg gtgtgagagg ggcggaaggt cagttggctt
    31501 tcccctctac tcgattatta tattttctta tcaataatag gaacctgatt ttaaaagcgg
    31561 ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg taaatggctt
    31621 ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg agaaaagcgt
    31681 attccatctc ttgtccattg aataagatct ggatacgata atgatttttg aaacgcttct
    31741 gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa agaaaagtcc
    31801 ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat gtataaaaac
    31861 tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta tatagacagg
    31921 aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt gcatcttcca
    31981 tcggcaagtg cctcctcgaa cccattatcc aaatttttag ttaattctaa tataccatca
    32041 ttaagtataa atgtaaacgc ttgttgatgg tataagattt tcgtttctga taaaacacga
    32101 acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata aaaagatgat
    32161 tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt aatgtaaata
    32221 aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag ccaaaaaaat
    32281 atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag caactgcgtt
    32341 gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc aaatcacaag
    32401 tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg aacaattgtc
    32461 taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg ttatccgaaa
    32521 tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag aattcatcaa
    32581 accaatcatt gaattctact ataaggatga ccagttggat gatccattta tcaatgaaga
    32641 gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc agtgagctga aagcccaagc
    32701 gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg ttttagtaga
    32761 tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg aagtaacacc
    32821 ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag acgtttaccg
    32881 cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc tatcggaact
    32941 aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac ctataaagat
    33001 tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat gggatatgaa
    33061 acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga tgcagaagaa
    33121 ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt aaatatggaa
    33181 acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg cggtcatcgt
    33241 ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga tcatgggcgc
    33301 tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg gggttttact
    33361 gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc ggttttctac
    33421 aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg gtacatgcaa
    33481 tggattccag atattgacag aagcaggact tttgccaggt gtactgagac gaaatgagtc
    33541 tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg cctttacttc
    33601 agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag gaaattattt
    33661 ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct ttacttataa
    33721 tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag cagggaatgt
    33781 gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat cagaagatgg
    33841 gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat agatgaaaac
    33901 attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg aatggggatt
    33961 gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc ctaattatac
    34021 ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa attctaaacc
    34081 ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg gagaaggcgc
    34141 aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa gtcacaatca
    34201 tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa tcatcagaga
    34261 tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt tcggcgaatt
    34321 agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg gcggatacgg
    34381 caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt gttatgaagg
    34441 aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata tccagaaagg
    34501 gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag gtcgcgacgg
    34561 tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac agcaacgttc
    34621 tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt gtttggaatt
    34681 aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag caggacttgt
    34741 ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga cattagatga
    34801 agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg aatcacaaga
    34861 acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac tatttcagaa
    34921 gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat atcggctata
    34981 tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag atgcaccagt
    35041 ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt tagctcctta
    35101 tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc aaccgacgat
    35161 tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca atacagttgt
    35221 ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag cgttagctat
    35281 gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag gacaaatagc
    35341 agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg cgatcaccga
    35401 ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat cgacttcagc
    35461 tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag gaaatgtctc
    35521 tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg gtatggtcgg
    35581 attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag gggatctaat
    35641 ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa agatgcagct
    35701 tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga gaaaccaaga
    35761 gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact gttcagaagg
    35821 tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga tatctgtaca
    35881 gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg tgttgacagt
    35941 cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg ttttagcagg
    36001 taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga tcaaaataga
    36061 aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga agtgaagagt
    36121 ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc tagtgtggca
    36181 tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat cgtgtccaat
    36241 gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt attcaaagat
    36301 caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg ttatgcaaca
    36361 gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca tgatcagtcg
    36421 atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa agagttagag
    36481 gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca tttgatccga
    36541 cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga agtcaaaggt
    36601 ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga cccaaatggt
    36661 tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc ctcggaaact
    36721 tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg tgagatagtg
    36781 atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca gctttctatt
    36841 tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg gatcaatgtg
    36901 catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt aaaagcagat
    36961 atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc ggaagctagt
    37021 ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac atttatccag
    37081 ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt acgaggagta
    37141 gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac gacaagcaga
    37201 cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag gattgcttct
    37261 cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa agaactcatt
    37321 gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc tttaggtttt
    37381 ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc gtattcagga
    37441 ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta tgaagaaaaa
    37501 tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa gaagggagta
    37561 tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag ctggatatga
    37621 agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta tgatgggcgc
    37681 tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag agccagtcct
    37741 tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag cagacaaaca
    37801 cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag cacaaggcgc
    37861 agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt tacgtttgga
    37921 aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc tgattggcgg
    37981 agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag ccggatttgc
    38041 tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc caggagatgt
    38101 gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg ttcgcaaaat
    38161 tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga aaaaaacact
    38221 tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc cgctgatcaa
    38281 agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag aaaatatccc
    38341 gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc cgattcttcc
    38401 aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt atgaaatctt
    38461 caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag aaatagtaca
    38521 cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg ccaaagaaaa
    38581 acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc ttctggaaac
    38641 ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga atcttcgatt
    38701 gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac tgcgttgagt
    38761 gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta tgaagaagca
    38821 attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg atatatgcga
    38881 atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat ccatccgtct
    38941 cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc tggagtaaaa
    39001 gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc aatcatccgg
    39061 caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga aaaaatccat
    39121 agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa tggaggctat
    39181 ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta gcattcgccc
    39241 aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag catgcgttgg
    39301 ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct gaaatgatgg
    39361 atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga agacgcgatc
    39421 tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac tttgtttgtg
    39481 tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata gcagaagcta
    39541 ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa aaccatgaat
    39601 ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa ttacgtcagt
    39661 atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt cgtcatactg
    39721 cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa aaagaaccgg
    39781 aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa aatagccatc
    39841 aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca gcagcaaaac
    39901 aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct gcgctacgga
    39961 tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat ccttgtggta
    40021 ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca gatcctgttt
    40081 caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca gcagaacaga
    40141 tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa gctttggaat
    40201 tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag gaagaaaatc
    40261 aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat caagatctgg
    40321 caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact ccagcagaag
    40381 caaaagcatt agcgtttgct tggagagctg tcaaacatgt gaaatctaat gcgatcgtac
    40441 tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga gtcggttcag
    40501 taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta gctagcgatg
    40561 cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata aaagcaatca
    40621 ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat aaatacgatc
    40681 tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc ggataaatga
    40741 acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa cttttagcca
    40801 gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca aaagatggta
    40861 tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt gttaagaaag
    40921 aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg atcgtcgatg
    40981 attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct ctgatcgaag
    41041 gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca gctaaatcca
    41101 gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt gcgccaatcg
    41161 tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc acagtgaagg
    41221 aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat agtagcaaaa
    41281 aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg tttgtccgta
    41341 ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt gataacgata
    41401 aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt tcaaaaaaga
    41461 tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg aaagaactag
    41521 gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa ggaccaaaag
    41581 tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt tctcggttga
    41641 agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta gagttaaagt
    41701 ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac cccgaagaat
    41761 atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt tactatgctg
    41821 gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat ttagtagaag
    41881 catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta atggataaaa
    41941 cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat agcgaactga
    42001 tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa tcggaagcgc
    42061 cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt atataaataa
    42121 ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc agctgcagtg
    42181 atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat tgagacagct
    42241 tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg tgaatgtgaa
    42301 gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct ttttattcaa
    42361 acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca tatcattgaa
    42421 tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt acttcttcat
    42481 cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga attggaacgg
    42541 tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat cgaattgctg
    42601 aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt gaaacatact
    42661 ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat cgatcatgac
    42721 ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg gtcaccatac
    42781 cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga attaaaccaa
    42841 acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc ttctgcttgg
    42901 attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt agggcgaatc
    42961 aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta cgaattgtac
    43021 cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa gggctataaa
    43081 ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg tctttttt
//
LOCUS       DADXTH010000013        42879 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.013, whole genome shotgun sequence.
ACCESSION   DADXTH010000013 DADXTH010000000
VERSION     DADXTH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.013"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(9..125)
                     /locus_tag="IWI97_000684"
     CDS             complement(9..125)
                     /locus_tag="IWI97_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBC2462212.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(198..1154)
                     /locus_tag="IWI97_000685"
     CDS             complement(198..1154)
                     /locus_tag="IWI97_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBC2462213.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1325..1693
                     /locus_tag="IWI97_000686"
     CDS             1325..1693
                     /locus_tag="IWI97_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBC2462214.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1863..2294
                     /gene="mraZ"
                     /locus_tag="IWI97_000687"
     CDS             1863..2294
                     /gene="mraZ"
                     /locus_tag="IWI97_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBC2462215.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2315..3274
                     /gene="rsmH"
                     /locus_tag="IWI97_000688"
     CDS             2315..3274
                     /gene="rsmH"
                     /locus_tag="IWI97_000688"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBC2462216.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3279..3680
                     /gene="ftsL"
                     /locus_tag="IWI97_000689"
     CDS             3279..3680
                     /gene="ftsL"
                     /locus_tag="IWI97_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBC2462217.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3681..5873
                     /locus_tag="IWI97_000690"
     CDS             3681..5873
                     /locus_tag="IWI97_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBC2462218.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSVYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5899..6861
                     /locus_tag="IWI97_000691"
     CDS             5899..6861
                     /locus_tag="IWI97_000691"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBC2462219.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6875..8236
                     /locus_tag="IWI97_000692"
     CDS             6875..8236
                     /locus_tag="IWI97_000692"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBC2462220.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8262..9350
                     /gene="murG"
                     /locus_tag="IWI97_000693"
     CDS             8262..9350
                     /gene="murG"
                     /locus_tag="IWI97_000693"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBC2462221.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9365..10585
                     /locus_tag="IWI97_000694"
     CDS             9365..10585
                     /locus_tag="IWI97_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBC2462222.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10741..12066
                     /gene="ftsA"
                     /locus_tag="IWI97_000695"
     CDS             10741..12066
                     /gene="ftsA"
                     /locus_tag="IWI97_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBC2462223.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            12089..13330
                     /gene="ftsZ"
                     /locus_tag="IWI97_000696"
     CDS             12089..13330
                     /gene="ftsZ"
                     /locus_tag="IWI97_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBC2462224.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13347..14024
                     /locus_tag="IWI97_000697"
     CDS             13347..14024
                     /locus_tag="IWI97_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBC2462225.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            14038..14637
                     /locus_tag="IWI97_000698"
     CDS             14038..14637
                     /locus_tag="IWI97_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBC2462226.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14652..14939
                     /locus_tag="IWI97_000699"
     CDS             14652..14939
                     /locus_tag="IWI97_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBC2462227.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14988..15770
                     /locus_tag="IWI97_000700"
     CDS             14988..15770
                     /locus_tag="IWI97_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBC2462228.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15791..16498
                     /locus_tag="IWI97_000701"
     CDS             15791..16498
                     /locus_tag="IWI97_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBC2462229.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16760..19558
                     /gene="ileS"
                     /locus_tag="IWI97_000702"
     CDS             16760..19558
                     /gene="ileS"
                     /locus_tag="IWI97_000702"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBC2462230.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19607..21133)
                     /locus_tag="IWI97_000703"
     CDS             complement(19607..21133)
                     /locus_tag="IWI97_000703"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBC2462231.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(21148..21795)
                     /locus_tag="IWI97_000704"
     CDS             complement(21148..21795)
                     /locus_tag="IWI97_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBC2462232.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21979..22308)
                     /locus_tag="IWI97_000705"
     CDS             complement(21979..22308)
                     /locus_tag="IWI97_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBC2462233.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22485..23213
                     /gene="sfsA"
                     /locus_tag="IWI97_000706"
     CDS             22485..23213
                     /gene="sfsA"
                     /locus_tag="IWI97_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBC2462234.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            23229..24242
                     /locus_tag="IWI97_000707"
     CDS             23229..24242
                     /locus_tag="IWI97_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2462235.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            24242..25518
                     /locus_tag="IWI97_000708"
                     /pseudo
     CDS             24242..25518
                     /locus_tag="IWI97_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            25581..28283
                     /locus_tag="IWI97_000709"
     CDS             25581..28283
                     /locus_tag="IWI97_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2462236.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28435..28752
                     /locus_tag="IWI97_000710"
     CDS             28435..28752
                     /locus_tag="IWI97_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2462237.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28782..29102
                     /locus_tag="IWI97_000711"
     CDS             28782..29102
                     /locus_tag="IWI97_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBC2462238.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            29210..30670
                     /locus_tag="IWI97_000712"
     CDS             29210..30670
                     /locus_tag="IWI97_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2462239.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30738..30959
                     /locus_tag="IWI97_000713"
     CDS             30738..30959
                     /locus_tag="IWI97_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462240.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30990..31172
                     /locus_tag="IWI97_000714"
     CDS             30990..31172
                     /locus_tag="IWI97_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBC2462241.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            31172..31585
                     /locus_tag="IWI97_000715"
     CDS             31172..31585
                     /locus_tag="IWI97_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBC2462242.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31708..32889
                     /locus_tag="IWI97_000716"
     CDS             31708..32889
                     /locus_tag="IWI97_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2462243.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(32960..33124)
                     /locus_tag="IWI97_000717"
     CDS             complement(32960..33124)
                     /locus_tag="IWI97_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462244.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            33278..33351
                     /locus_tag="IWI97_000718"
     tRNA            33278..33351
                     /locus_tag="IWI97_000718"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33312..33314,aa:Arg,seq:cct)
     gene            complement(33420..34559)
                     /locus_tag="IWI97_000719"
     CDS             complement(33420..34559)
                     /locus_tag="IWI97_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2462245.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(34858..35493)
                     /locus_tag="IWI97_000720"
     CDS             complement(34858..35493)
                     /locus_tag="IWI97_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462246.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEEINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(35606..36241)
                     /locus_tag="IWI97_000721"
     CDS             complement(35606..36241)
                     /locus_tag="IWI97_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBC2462247.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(36275..36736)
                     /locus_tag="IWI97_000722"
     CDS             complement(36275..36736)
                     /locus_tag="IWI97_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBC2462248.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(36866..37297)
                     /locus_tag="IWI97_000723"
     CDS             complement(36866..37297)
                     /locus_tag="IWI97_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBC2462249.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(37315..37635)
                     /locus_tag="IWI97_000724"
     CDS             complement(37315..37635)
                     /locus_tag="IWI97_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2462250.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            37934..38710
                     /locus_tag="IWI97_000725"
     CDS             37934..38710
                     /locus_tag="IWI97_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBC2462251.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            38725..38928
                     /locus_tag="IWI97_000726"
     CDS             38725..38928
                     /locus_tag="IWI97_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462252.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            38944..39264
                     /locus_tag="IWI97_000727"
     CDS             38944..39264
                     /locus_tag="IWI97_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBC2462253.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            39269..39439
                     /locus_tag="IWI97_000728"
     CDS             39269..39439
                     /locus_tag="IWI97_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462254.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(39446..40228)
                     /locus_tag="IWI97_000729"
     CDS             complement(39446..40228)
                     /locus_tag="IWI97_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462255.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLCYLLQFLIN"
     gene            40279..40986
                     /locus_tag="IWI97_000730"
                     /pseudo
     CDS             40279..40986
                     /locus_tag="IWI97_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
     gene            41164..41505
                     /locus_tag="IWI97_000731"
     CDS             41164..41505
                     /locus_tag="IWI97_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462256.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            41498..42169
                     /locus_tag="IWI97_000732"
     CDS             41498..42169
                     /locus_tag="IWI97_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBC2462257.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            42175..42861
                     /locus_tag="IWI97_000733"
     CDS             42175..42861
                     /locus_tag="IWI97_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462258.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
BASE COUNT        14346 a         7227 c         8687 g        12619 t
ORIGIN      
        1 ttttattttt acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc
       61 agcattgtcc aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca
      121 cgcattgttt tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt
      181 tccacaagag ggctttttta taaaaacttc ccttttttta aataataggt agttacgata
      241 caaataaata atgttaatag aactagccat ataaaggcat catccctttc agcaataggt
      301 aacttaatgt tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt
      361 aaagaagtta aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc
      421 tgattcattt gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt
      481 tccaccaata catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt
      541 tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg
      601 cttttttgga tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg
      661 gcttccaaag tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg
      721 acctgccaaa tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag
      781 gtctggctaa agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc
      841 tttttgggcg tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga
      901 cttgttttag caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga
      961 ttgaattctt ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac
     1021 ggaatttcaa attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa
     1081 taccagatcg tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta
     1141 aaatatttga tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa
     1201 ttggaataca tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa
     1261 tgaaacagtt gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac
     1321 gttcatgggc acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt
     1381 gtttggttgc caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc
     1441 ccctgatttg gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg
     1501 gacaatttct atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc
     1561 tgttttccaa gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg
     1621 gcgtttggtt tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact
     1681 ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc
     1741 ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg
     1801 tggtagaaag tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt
     1861 caatgttgat gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct
     1921 caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc
     1981 ttttcggcta tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt
     2041 tggctaaaaa agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg
     2101 aacttgataa acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga
     2161 caaaaggttg cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat
     2221 ggcaagattt ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg
     2281 atttcggact ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac
     2341 agtcatgttg aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga
     2401 ctgtacactc ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg
     2461 gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc
     2521 ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga
     2581 acgcttgagt gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc
     2641 tcctcagcta gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat
     2701 gcgcatggat cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca
     2761 tgaattagta aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg
     2821 tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat
     2881 cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat
     2941 ttttcaagct gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga
     3001 acaggcaatc cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt
     3061 ggaagacaga attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc
     3121 gggacttcct gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc
     3181 aatccttcct tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg
     3241 tattgcagaa aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc
     3301 aagattacga atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt
     3361 cttcttcgaa tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt
     3421 ctgctttgga aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt
     3481 taacagttat gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa
     3541 cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt
     3601 ctcgtactga acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca
     3661 atttaaggaa agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga
     3721 aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta
     3781 ttggattgtt ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg
     3841 ctggtacctc attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag
     3901 caaaaagagg aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt
     3961 attcaattaa agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg
     4021 aagaaaagaa ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa
     4081 aagatgcact gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg
     4141 gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa
     4201 aagaaggtgt cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg
     4261 tcttttcttc tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg
     4321 gtaaactagg tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta
     4381 tttatcaaaa agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag
     4441 ctgtcgacgg acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa
     4501 cactgatgga tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga
     4561 aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa
     4621 tggaaggatt gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg
     4681 aaccaggatc aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca
     4741 acgagaatga aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc
     4801 atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg
     4861 tcgggatggt catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac
     4921 tgggatttgg acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta
     4981 cattaaatat tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt
     5041 tccaaatgat gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat
     5101 atatcagcaa agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc
     5161 tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg
     5221 tcgaaagcga aaactacggt agtgtttatg gtgtatatag tgtacctgga tacaatgtat
     5281 cggcaaaaac cggaacagcc caaatcgctt ctgatacagg ggggtatcaa acaggagata
     5341 cagcctacct gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt
     5401 atctaacaat gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc
     5461 cattgatgaa acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa
     5521 aaacagaaaa agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg
     5581 atgctcaaaa aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac
     5641 aatctactgc caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg
     5701 gtgataagct gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga
     5761 aaatcctcgg tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg
     5821 ctccatatga aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga
     5881 aataggagag aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt
     5941 gcaatgacaa ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt
     6001 caggcgattc gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg
     6061 ggcggcttag tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa
     6121 aaacagctaa ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc
     6181 ggctttcttg atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag
     6241 cagaaattga ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa
     6301 ggttatccag gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt
     6361 attttcgctc tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc
     6421 gatggattag ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg
     6481 catcagcatc agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt
     6541 ttcttcgtct acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg
     6601 ttgggcggac ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg
     6661 attggtttga tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag
     6721 cttacaggaa aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga
     6781 tggtcggagt ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc
     6841 acattatgga tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa
     6901 taagaaagtg cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca
     6961 tgaattgggc gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc
     7021 acaagagctg ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt
     7081 ggatgaagaa ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt
     7141 agcaaaagcc caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt
     7201 agcggaatgc ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat
     7261 gacaggacta ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat
     7321 tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga
     7381 attgtctagc ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac
     7441 caatatctat gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa
     7501 atggaacctt caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc
     7561 agaactacaa gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga
     7621 agtgttggaa gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat
     7681 aatggaaata tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc
     7741 tatttctgta gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt
     7801 tttccatggc gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta
     7861 taatgattca aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac
     7921 gtctaaggtc gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat
     7981 tccttcatta aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat
     8041 ggatgctgga aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc
     8101 agttccttta gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa
     8161 tgccagctgg gatcaatacc caaattttga aactagagga aacagattca tggaagctgt
     8221 gaaccgatta aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag
     8281 gcggaggaac agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc
     8341 aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg
     8401 tccctgatac aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt
     8461 ccatgcataa catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa
     8521 tcctcagtga ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg
     8581 tagtgtacgc tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac
     8641 caggtgttac caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag
     8701 atgcggcttc cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag
     8761 aagtagcaga tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga
     8821 aaacagtctt gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag
     8881 tttttttaag tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat
     8941 attatgaaga gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt
     9001 atatcaaaaa tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg
     9061 caacttcgat cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat
     9121 atgttacgaa tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa
     9181 aaatgattgc agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca
     9241 tgggtgatga agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg
     9301 atgcttcaaa acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt
     9361 cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa
     9421 tttgactcct tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca
     9481 agagccaaaa aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa
     9541 aacggttcaa gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga
     9601 agagatcaag caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc
     9661 caatttaaaa agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt
     9721 attgggtata ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc
     9781 agttgcagta tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt
     9841 gtcattagga gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct
     9901 taaaaaagca cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt
     9961 cgatttggat gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta
    10021 cccagtgatt gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct
    10081 ccctatcttg gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa
    10141 tcagttatca tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag
    10201 caataaaaat ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa
    10261 taatcttgcg aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg
    10321 tgtgatcgac atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga
    10381 agaatcgggc caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga
    10441 atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg
    10501 atcatctgat tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac
    10561 tccgtcaagt ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta
    10621 aaaaccagta atttatggta gaattggaag cagtgaatat atctattaat aagtatattt
    10681 taaaacggac gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc
    10741 atggcaaaaa caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc
    10801 gtggctgaat atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa
    10861 gggattaacc gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca
    10921 gtacgacaag cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca
    10981 gcaaacatgc ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag
    11041 gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct
    11101 cctgaacgtc aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga
    11161 atcaaagatc ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca
    11221 ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc
    11281 aacgaaatgg tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa
    11341 gattttggta cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat
    11401 gataagcaat tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat
    11461 atctctattg tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg
    11521 gatgcatacc cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag
    11581 tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa
    11641 cagattttca acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt
    11701 ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa
    11761 atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta
    11821 tttacaaatg taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg
    11881 gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa
    11941 acaacaaaca catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa
    12001 ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt
    12061 gattaattca tcctgggagg aataagatat ggaattctca attgataata atatcaatga
    12121 cggcgcagta atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat
    12181 gattgaagaa aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt
    12241 aaaaaattca aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg
    12301 tgctggttct cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg
    12361 cgaagcttta gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg
    12421 tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg
    12481 tgtcgtgaca cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg
    12541 tatcgctcgt ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt
    12601 agaagttgta gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt
    12661 acgacaaggt gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga
    12721 ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt
    12781 ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct
    12841 tgaaacatct attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat
    12901 gacattgttc gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt
    12961 aaatatcatt ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt
    13021 catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca
    13081 agcacaaatg caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac
    13141 ttcttcagaa gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg
    13201 tcctagagtt gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa
    13261 ccgcgaagaa acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg
    13321 taaaaaataa aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag
    13381 aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg
    13441 taacaaaatc tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt
    13501 gtgcagaaaa ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg
    13561 aaataaagtg gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg
    13621 aaattgatta ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt
    13681 tagagaaaga attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg
    13741 gaatttcacc agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca
    13801 aggttgttgg gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag
    13861 tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac
    13921 catgtagtga attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg
    13981 caacatttat ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg
    14041 ccgcttttaa acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac
    14101 gaataccaag attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca
    14161 acacagaatc aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg
    14221 gcgacttcac gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt
    14281 gaactacatc aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct
    14341 acacaaccta aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct
    14401 gaagctatgg cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat
    14461 ttagtagaag aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca
    14521 ctagatggag atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg
    14581 gagattgaca gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag
    14641 gaggaactac gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt
    14701 tctatagcat tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat
    14761 cagggctagg acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc
    14821 tgcctttgag gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact
    14881 tagccgtaca aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat
    14941 tgaagcaaca aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg
    15001 tataccagca ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg
    15061 aacaggttca aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct
    15121 atattttaga aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat
    15181 atgaacaggc agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg
    15241 aagattttga aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc
    15301 atgggaaaat ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata
    15361 tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg
    15421 ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag
    15481 aaatgattat gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac
    15541 tagacagtgt gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg
    15601 aagctggtaa agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac
    15661 tattagatat tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga
    15721 agacgaaaaa agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg
    15781 tggtttactc atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat
    15841 gcggggatat aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga
    15901 ggaattgact caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact
    15961 tgagtacttc aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac
    16021 ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca
    16081 aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga
    16141 tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc
    16201 gacagaaact gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat
    16261 gcttgagtct caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt
    16321 ctccagctac gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga
    16381 caatgaaaat gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc
    16441 atttgataca caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa
    16501 cagaaaagca ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac
    16561 tgcatggtga aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc
    16621 ggctgacctc gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt
    16681 ggtaccacgg catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa
    16741 taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt
    16801 ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa
    16861 tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga
    16921 tggacctcca tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa
    16981 agacattatc attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg
    17041 ggatacacat ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga
    17101 aatgtccatg gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa
    17161 gcaacgagca gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac
    17221 attagatcct tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg
    17281 ctatatttac aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc
    17341 ggaagctgaa attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt
    17401 caaagacggc aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc
    17461 atggacattg ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt
    17521 ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga
    17581 aatcggttgg gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat
    17641 gactgcaagt catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac
    17701 tttagacgct ggtactggat tagtccatac tgctccagga catggggaag atgactatat
    17761 cgctggtagc aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac
    17821 agaagaagca cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga
    17881 tctattaaaa gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca
    17941 cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat
    18001 cgataaattc cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg
    18061 gggtaaaaca cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca
    18121 gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac
    18181 accagaaaca atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt
    18241 tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg
    18301 cgaatttaca aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga
    18361 agctgtttta cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc
    18421 agaccaatat cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt
    18481 agcaccatac aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat
    18541 gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga
    18601 tatccttcgc ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga
    18661 tatcttaagt caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt
    18721 agcgaataca gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc
    18781 tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg
    18841 gtacgacaaa tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga
    18901 tctatcgtca ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga
    18961 ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt
    19021 attgactcct atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga
    19081 ggagtacgtt cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt
    19141 gatggatacc tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga
    19201 agcaagaaac agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa
    19261 acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt
    19321 ttcaccagac tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt
    19381 atttgatgat gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg
    19441 ccaagttcgt aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc
    19501 aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa
    19561 gggaaaaggg ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt
    19621 ttccacgttc acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa
    19681 gtagttcgaa agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt
    19741 gtgtttggtc ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt
    19801 cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag
    19861 cttctgggct attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg
    19921 tttcaaatcc ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct
    19981 gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag
    20041 atgttttgaa tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt
    20101 cagtcaaacg tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga
    20161 tagtgaattt gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt
    20221 cgcgatagcc cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga
    20281 agttttctaa cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt
    20341 ctgtacggat ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa
    20401 cctgtaaaat atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc
    20461 cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat
    20521 tgttccattg agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca
    20581 tttcttttcc taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga
    20641 tttgctggtt caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca
    20701 aacgattgaa gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc
    20761 caaaaaattg aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc
    20821 catctaatcg gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat
    20881 gggattgata gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg
    20941 cacggattgt ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg
    21001 ttccgattac agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg
    21061 ggtacaattt acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt
    21121 tttcattaga catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc
    21181 cttaaagctg acggggattt tctttctatc attttggata gtgatcggtc cttcataagc
    21241 tgcgatgtca acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc
    21301 caacaattct ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga
    21361 ctccaatgtt tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc
    21421 atgaggacaa tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga
    21481 agtcacatgt tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg
    21541 ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag
    21601 tccgcttgca gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac
    21661 aagtttagaa atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg
    21721 tttattgttg actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc
    21781 tcgatttggt gtcatattcg atcatccttt catggtaaaa aaccatcatt agtaagtgta
    21841 acaaaaaaaa cgttaattga ctatttttct aaataaaaaa caaaggattt ttagacgttt
    21901 ttacaacaaa aaacgaaaga tgatagagaa gactcattct ttcgtttata aaggatatat
    21961 atatacattg ggagggatct atttttcaga taacaactta tatgaagtgt ctgcagattc
    22021 gtcataaaga gtttgttcgg gataagaaaa gataagataa aaaccatact gatcacgtac
    22081 gactaaacga tattcatcta tttctaccac agatgtgcca agagactgac cgttgatcaa
    22141 aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc tggctatcac taaagaacca
    22201 tgtaccttga tactttgaga gtccttctga cttttccgct tgttttttct ttttcaagta
    22261 aataaaactt gagagaacgg taatgagtga accaagaaaa tagatcattt tttttatcac
    22321 caaatcactc ctttatgaga aaataatcgc atagacatat tctttcttct ttatttcatt
    22381 gtaccgaata aaagctaaaa tttctctaag actttcgact ggttttttta ctgtttttct
    22441 acaaattatg ataggataca tagtaggaag aaaggtgaca taatttgaag tatcagcaag
    22501 tggaaatagc tttttttatt gaccgcccga atcggtttat agcacattgt atgaaaaaag
    22561 acggagaaat aattattgcg catgtcaaaa atacaggtag aggaaaagag gtcctgatac
    22621 caggcgctga agtggctatt gtatttgctc ctggacctaa acggaaaacc gcctatgatc
    22681 tgattgctgt aaaaaagaag ggtgactggt ttaatatcga tagtcaattg cctaatcggc
    22741 tggcaattga tggtatttta gacggcacga tccttttacc aggattaact ggtccattta
    22801 cggtatacaa gcgtgaagta acttatcttc actccaaatt tgatatttat ttagagacgg
    22861 tggtcggaca aaaagcattt gttgaagtca aaggtatgac acttgaaaat aaagcgattg
    22921 gtgcttttcc tgatgcgccc acactcagag gattgaaaca tataggagaa ttaacgtatg
    22981 ccgcacaaga tggttatgcc gcttatgtct tgtttattgc tcagttcgaa catcttcatc
    23041 tggcaacgat ccatgaagaa atgcaaccag cactagctga tatggttcga catgcgcagc
    23101 aaagcggagt tcaaatcctc gcctataatt gtcaagtatc ggcagatgag gtaactgtca
    23161 agcaggccat tccatttgat gtagatatcc cttttgaaga tcctatacaa tagaaagaag
    23221 gtaaaataat gatcaactta ggtattatcg gaacaaattg gattactcat caatttgtcc
    23281 aagcagcttt aagcacaaaa aaatatgaat tgacagcggt atattctaga catttggaaa
    23341 aagcacagga atttggttct cattatgaag gggatatcga attcgcaaca gatttgaaga
    23401 cttttttcaa tattactcat atggacacca tttatattgc ttctccgaat agtcttcact
    23461 ttagtcaagc taagcaagca attttggcag gaaaaaatgt gatagttgaa aagccagctt
    23521 tttcgacgcc aaaagaaatg gatgaaatca tccatttggc aaatgaacac aatgtgtatt
    23581 tttttgaagc agcaagaaat atccatgaat caggttttaa aaagattgcc gattacttac
    23641 cattaaaaga cgagataatc ggtgcaaatt tcagctatat gaaatattct tcgcgctatg
    23701 accaagtatt agatggagaa gaaccgaata tcttctcgac tcatttctca ggtggtgcat
    23761 tgatggattt aggtgtttac cttgtatatg cagctgttgc ttggtttgga atgccaaaag
    23821 aagtccatta tttcccaaga aagatttcta ctggtgtaga tggtcttgga tgggggatct
    23881 tacgttatga gaattttgat gtatcgatcc agccaggtaa aatcggtgat tcttatttac
    23941 cgtcagagat ttattttgac tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac
    24001 aagcacaatt tcatgatcgc gcgcataaag aaatcattga tttggcggta acagctgcag
    24061 aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt catggctcat ccgaaagatc
    24121 cagcttgggg attgttgtat gaagagtgga tcgagctagc tagaaatgtg aaccaagtga
    24181 tttatgattt aagaaaaaat ggcggcatca aattcgatgc agataatgag gaagattttt
    24241 aatgaacgaa tttgtacaag tatttcccga aaattggcag aaaaaatatg aggataaagg
    24301 gtttatctca ccttctctta ttcaacgagc agtttttcag ccactaagta ataaacaaag
    24361 tatagtagcg gtttctccta caggttcagg taaaacactg gcttatcttt ggcctctact
    24421 actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt ctcaagaact
    24481 agctattcaa gtagcagatg ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca
    24541 atctttggtg ggcggagcca acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga
    24601 aatcttgatt ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa aataaaagca
    24661 catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc
    24721 caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca
    24781 gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca
    24841 attgacgtga cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta
    24901 ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt
    24961 ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga
    25021 ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta
    25081 gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt
    25141 ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct
    25201 tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc
    25261 gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt
    25321 gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca
    25381 gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa
    25441 tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa
    25501 ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg
    25561 gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag
    25621 aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca
    25681 atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc
    25741 gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact
    25801 cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca
    25861 tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg
    25921 gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt
    25981 cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa
    26041 atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag
    26101 ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga
    26161 tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt
    26221 accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga
    26281 cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca
    26341 ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta
    26401 tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg
    26461 ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag
    26521 tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa
    26581 aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac
    26641 gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg
    26701 atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa
    26761 aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa
    26821 aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca
    26881 aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa
    26941 aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa
    27001 gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac
    27061 ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag
    27121 aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca
    27181 atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg
    27241 gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag
    27301 ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac
    27361 aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg
    27421 gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta
    27481 tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa
    27541 gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt
    27601 tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta
    27661 ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata
    27721 aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg
    27781 ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa
    27841 atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc
    27901 gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa
    27961 gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc
    28021 agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag
    28081 ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta
    28141 ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag
    28201 aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc
    28261 ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt
    28321 gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat
    28381 tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca
    28441 aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa
    28501 atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca
    28561 gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta
    28621 cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc
    28681 atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat
    28741 ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc
    28801 tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag
    28861 cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag
    28921 aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag
    28981 gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta
    29041 ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat
    29101 aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt
    29161 tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact
    29221 atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca
    29281 tttagttgct ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt
    29341 cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc
    29401 tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa
    29461 tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg
    29521 gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc
    29581 taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt
    29641 tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg
    29701 cacactgtca gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta
    29761 ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc
    29821 caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc
    29881 cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg
    29941 tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct
    30001 aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg
    30061 gttctttggt attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca
    30121 agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc
    30181 agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga
    30241 tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat
    30301 tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt
    30361 caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat
    30421 cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg
    30481 cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt
    30541 cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac
    30601 tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga
    30661 acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt
    30721 ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag
    30781 aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa
    30841 tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg
    30901 aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga
    30961 aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta
    31021 ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata
    31081 gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa
    31141 aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc
    31201 agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac
    31261 cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca
    31321 aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg
    31381 tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc
    31441 gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa
    31501 tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct
    31561 agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa
    31621 atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact
    31681 aacttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct
    31741 tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa
    31801 aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag
    31861 gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa
    31921 gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc
    31981 gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta
    32041 tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt
    32101 gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga
    32161 aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag
    32221 atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc
    32281 aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga
    32341 tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat
    32401 catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca
    32461 tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt
    32521 gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg
    32581 gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat
    32641 tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc
    32701 tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag
    32761 ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca
    32821 aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac
    32881 tatttttgat aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt
    32941 tggcatttga aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt
    33001 tttcttttgt tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt
    33061 cccgattttt tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat
    33121 tcattggaaa ggacctccct acattatttc attctacctc tagtttatca tctgttaagg
    33181 actagtggaa acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag
    33241 taaaaaacaa acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag
    33301 agcaatcgcc tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa
    33361 cccctgtatc ctttgcggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac
    33421 tagttatcat ttttatataa actatcgaac acagtattga ttttttcttt atcctgatct
    33481 tcaagctctc gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct
    33541 aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgtgta
    33601 tgccttagtc catgcaaagt aaattgcttt tcaatcccta attcttttaa ttttttgcgc
    33661 aaatatttgc tgacggcgtt tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa
    33721 ttatgagggt ttttgatttc aaaattctcg aataattctt tttgattgat tttaaacttt
    33781 tttaagagat caatcgtatg tttatcaatg gaaattttcc tatttgaagt ttcgtttttt
    33841 gtttttcccc attcatttaa tttataattc catgttttat taatagtgat gatttgatct
    33901 tcgaaatcga tatcttccca tgttaatcct agaagttcag caaatcgcat tccagtggca
    33961 ccagcaacta acacgagcat aggagaagag tactgcgcag aaaggttttc ttctacgagt
    34021 ttcattaaag atttaaattg atcataatct aaatatttat cttcttttgg cttcaatgaa
    34081 tcttttcctt tgattactgc ttttctagtt ggatcaaacg gaattaatcc ttcttctacg
    34141 gcatctttca acgacgctct aatatggtta ttaaacttaa taacggtaga ttttacatgg
    34201 tctttagcat acttatttaa aaagcgttga tatcccactc tatctaaatc agaaatcaat
    34261 accgctggca tatatttctt tatgttcatt aacgtatctt cgtattttct ataagtgata
    34321 ggagatactg ttccttcttt ataaagttgc atccaatcct caaagtaatc tgataataat
    34381 agatttttgc gatccccttt aaggcctttg ttcaagttat attctaattc gttggcggca
    34441 tcttttgctt ctccttttgt cttaaatcct gattttctta tcttcgtgta ttttccgtca
    34501 tcttttttgt atgaaatttc gtattgccaa ctatttccac gtttgactaa tcttgccata
    34561 attgaatcag ctctctttct ctgatacaat aggaactata aagaagccca ttgtttgggt
    34621 tttattattt tttgcacatc ctttttgctt gccggcgcaa ggatgtgcta tttgtcgttt
    34681 taatggtata attggatgtg tattagatca tccaatcaga ggtgtttatt atgattaaag
    34741 tgatttttaa aagaataact gaacttccag agtttagatt atttgttgat tattggtatg
    34801 tctggttttt tattgttgca ttgctgatag gtctagatgt catattagaa agaaaactta
    34861 agtaagagtt gagttaataa attgaattcc taaatgagat attttgtagc ccatattttg
    34921 tttgccgaca tcacctaata ctatggagct aatcgttttt tctaacagac caaaatcaga
    34981 taatctattt aagctaccat ctattagctc ttgtgaatag ccagacatgc tgatatttcc
    35041 agtgaaattt tcgtttccac gactcattaa gcatgctaac aattgaatct caatcgatgt
    35101 taattgattc agtacaagaa gataatactc ctcgttatcc catgaagcgt tgttaattcg
    35161 aagtaaacaa ttcttataaa gatttttata catatctatt ttattttgtg cctttgattt
    35221 ttctacctcg tcatttatct cttcgaatat tccaatgagt tggtccttgt tctctaagtt
    35281 gaaaacatct tctggaattt gatcgcgaat gttttctaat gattcgttta aatttttgta
    35341 gaatttctcg atgcgtttga aacgcttctc gtttttagac ccaaaataaa gtgactgcac
    35401 aacgccacca atataaggaa ttgctccaat accgccttca actattaatt caagatattc
    35461 ttttttggtt agtttttcat tgctattatc catagttacc tcctattttg tcatttcctt
    35521 tacaagcatt gtgactcctg atggcactct tttgtatagg ttttattatt gtgcacaatc
    35581 taactcttag tggggcgtgg ttcatttatt ttttacttac tttactatta atcatatccc
    35641 attgagcata ggatttttct atgttcgatc ctcttgaact aatttctcct gtaaaagtta
    35701 ctttatcgcc ctttgtaaag gtatcagcat tgtttaagtc cttcgcaaaa attacatatg
    35761 aaactttagg tgttgcagaa atatcattcc aacttttatc atcgtactct ttgtctgcta
    35821 taatggctat tctagtagtc attgattcga taacagttcc ttcaaaagta tatgttttac
    35881 cactaatata tttagtaaaa gctgtatttt gctcagtagc tggagtaata gaataataac
    35941 tatctaagaa agcaccccag tcatgattat ttgtctctaa aaattcttca aattgagtta
    36001 ggcttttatt gctgctgtta gatgtagtag tttgagtatc agatgatgag ctttcctgtt
    36061 tgttattgtc atttgcctcg tttgatgagg aaagcgagaa acctacggta gtaaaaataa
    36121 tcccgataat aaggagaatc ataggtgttt tcttatttgt ttttttgata aatcttacaa
    36181 ttaacatgat taaacctata ataaataaaa caataccagt aaaaccaaat ataccgtcca
    36241 ttaatagatc ctctttctct tttgatattg aatattataa accaagcaat tcttttttct
    36301 taaaatcgaa ttcttcttga ttaataatcc cttcatctaa caactcttta tactttttaa
    36361 tttcatctgc acttgaaatt tgtgtttctt tggaattatt atctaatata gattgttgct
    36421 tctctatata tttttttaaa ttttccattt cattccaata tttttttgta aacataacgg
    36481 tgttttcatc tttcgtggcc gctaatatac ctccgcgact ttcgtttccg cctagcaagg
    36541 taaattgtat ataaccatta ctcatacctg gtttttttaa ttgtacggct gatatatttt
    36601 taaaggggat agttttttct cctttaatac cttgattaac taaatttata aatccttttc
    36661 taataattga aatagaatca tcagttactt taatcaaggt ttttcctgga gattttatta
    36721 aaatctcttt ttccattttt tcttcctcat ttctgtgata tgattttttt tgtagaagct
    36781 catatatgag gaaagagccc gtgttgcagc acgggctttt ttaatgtttt gctgaaatcg
    36841 gtttttttaa taattcttga tgtattcata catattgcct tgcgtaagaa tattttttct
    36901 taaaatagca ttggcagaca gcataacaag tgtatctgag cttattctga atagaataat
    36961 actccataaa tttttcgaga ttaaactgag attcatcagt cagttcgttc tcaatataga
    37021 tatttaataa aattagaata gctattttat ctgcttctgt ttcaaatttt gagtgaaaag
    37081 ttgtagaagt atcgtacaaa actgaaaatt caaaatgaga ggcgctgaaa tgtgcaagtt
    37141 catgagataa atgaaaggct tctgcagttt caccgtatag attttcattc aaaaaaatga
    37201 ttctgggttt tggatagtag aaaccaggct ctttcatctc catataaact actttcaaat
    37261 tgtattcgct caacatttct ttcaatttca aatacataca aaccatcact ccaactattc
    37321 attttcctct aaagctttag caattgcaat cgctttacgc attgtctcct tagatatttc
    37381 ttttccatca aaagaaaaaa cagtatcgtc ttctgataaa tccacatgtt taggggtttc
    37441 tctttcttct ctacctagaa ggtagtctac agagacatcg aaatagtcag caatccgact
    37501 taattcatct gaattaggag tattattttt ccactttgct agataaccgt tagaatatcc
    37561 aaaattaatt tctaactgcc gtatagatat ctttttcctc ttcgccaagt cttttattat
    37621 ttcataggta ttcatcgata aaacaacctt tctaaatgtt tacaaaaaaa gtttagaaaa
    37681 atacgcagaa ataatttgac taattctgag taatgagcta tactatatct tgtaaacaga
    37741 tttaacaact aaaaagacaa caaaaaacac tattgatatg taaatgcaga ccgccaagaa
    37801 agctttaaaa tcaatgtttt tatgtcttat ttaattatgc tcttagtata gagtattact
    37861 cagatactgt caattgaatt tagaaaaaag ttgttaaatt tgtttacgaa tataaaagaa
    37921 aggaatgaga aatatgaaca caccgcaaat ttttaatttc gaacaaaacg aagttcggac
    37981 aattttagta aatgatgaac catattttgt aggcaaagac gttgcgagtg ttttgggtta
    38041 ttcaaacact aaagatgctt tgtcgcggca tgtagatttg gaagataaga tggggtcgcg
    38101 aatcacgacc tcaggtcaat caagagagat gacaatcatc aacgaatctg gtttgtacag
    38161 tttaatctta aaatcaaaac ttccctctgc caaaaaattt aaacgttggg taacaagtga
    38221 agtgttgcca gcaattagaa aacatggagg ttatctaact ccagaaaaag tagaagaagc
    38281 tttgcttaat ccagatacaa tcattcaatt agcaactcaa ctaaaagaag aaagaactgg
    38341 aagattaatc gcagaacaaa agattgcaga gtacgaacct aaaatctcct atttagatag
    38401 catattatct tctacagatt cagtaacaat tagtcagatt gcagcagatt atgggatgtc
    38461 tccacaacag atgaataaat tacttcataa actaggtatt cagaaaaaag tcggtaacca
    38521 atggttattg tgcaaaaaac acatgaacca aggatacaca aaatctcata caactgagat
    38581 cccgaaagcc gatggtggca ctaaaattgt aatgaatacc aaatggacac agaaagggcg
    38641 tctatttatc tacgaattac taaaaaaaga aggatattac cctcaaatgg atttagagga
    38701 aattggttag aaaggagttt tagtatgact gacattgcag aaatcactca acgagataga
    38761 gaaaaaatca aagaatatgt cgaaagttca aagttcttaa cttacaccat gcttgctgaa
    38821 agatttggaa ttagcaaaag ctacttatct ttaattttaa acggtaaaaa gacttctgca
    38881 gaagcaaaca gaattataga ttcgattatc actatgtacg aattgtaaag ggaggaacag
    38941 ctaatgcaat atctagaagc aaaaatccca attccagaag gctatgtaat tatctcccaa
    39001 gtggattatg aggagttaaa aaaagctgat gatactggta gatggatgac gttgccagaa
    39061 gtgctagaac ggattaacag aaaatatgat tggtttactt ctagagtttt aaagaaccca
    39121 agatatagaa atattatcga tatcgagaaa aacaaaaatg gatttgtcta ttatccagtt
    39181 gaaggaagag acacatatct atttttaaga agtaaaacac ttgaattttt agaaacaaat
    39241 ttttcggaaa tcttaaggag gtaagcggat gggtaaattt aatagagcac tagtattcag
    39301 cgcaccacta attatctacg ctttaggtct ttggggaagc agacaagcat taatcggcac
    39361 aattgtatat atggtctgga tctttatggg gcttgatgaa gctgaatata gagcgaaaaa
    39421 gccagtcggg agggactgac ttaacctaat taattaaaaa ttgtaacaaa taacacaaaa
    39481 ataaagctaa tgaaattgta ttgttaggat ctactccaat agttatattt acaatttcgg
    39541 ttactactag tcctatcaaa gctgccccag tttgctctaa ataccacatt ttatctttgt
    39601 agaaagcagg tgaagcttgt tgtttttcag atggttcatt agttatatct ttgttcttgg
    39661 ctgtatttat ttcttcaaaa agctttatag aatcatcaag gactttcctt tgctcattaa
    39721 ggatatcaaa aaacttagcc tcaaactcat cagatggatt agaagtgtct ttaaaaagta
    39781 gattttttgt actaacttcc ggttcggaag aaaatgcttt ttctaattct tgggtagcta
    39841 cccctattct gaagtcgtac ttagacatgt caacggcaaa tttttctgtg attttagaga
    39901 tatcagaaaa ataaggtttt ggcatagtag ctgccatttt tttagcaata ttaaaacttg
    39961 gagattctaa ttgtttctgt attgcgaaca aagaaggagg aatcatatta gcaatagatt
    40021 tgttacgttt ctcgacaatt tgaatgagag aatcattgag tattgcttgc gaagctataa
    40081 aagtatcttg aattgcaatt aattttttag cttgttctat cgcaggctgc atagcgttaa
    40141 cgacatctag agaaggagca atggtttcac cgattaattt tgaagaagct atcaattttt
    40201 gataataagg agtatctgaa atatccaatt atatacacca cctttttacc taaattatac
    40261 caaaagggag agaaaaaaat gcaataatta gtaattttga aaaataaaga agctgtgact
    40321 acgagcttac aagtcgcaga aacattccga aaggaacata gagcagtttt aaaggcgatt
    40381 gacgatctaa aagatgggct tgcacaaaag tatgcagacc tattttggga agatgtgtac
    40441 atccatccgc aaaacaaaca ggcttatcga attgtctata tgaatcgtga tggctttact
    40501 cttgctgtat taggattcaa caatactcaa aaagttttag agttcaaatt gaaatacatt
    40561 gaagcgttca acgaaatgga aaaggcaatt cgcaaaaata cagcttttga cacaattgaa
    40621 gacatgatga tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt
    40681 aaaaatacta tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatacg
    40741 aaagttatgg aagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa
    40801 gtattctcaa aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag
    40861 ttacctcgta agagattcga tgatgctgtt tcatttattg aaatgtggtt accagaaact
    40921 gcgattcgca tggaaatcga tcaactgaac agacaacaaa gactttttgg tgatgaaaat
    40981 gaatagagct gaagcgctaa gaatcgggac ggtaattgct aatcgctggt ggagacacaa
    41041 taaaccaatc atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa
    41101 aaagtgactc cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag
    41161 catatggaaa aagaactttc cactctagat caatatttga ctgatcctag ttggggcaaa
    41221 tcgaatatca aggaaacaaa taatcgaaaa atcagacgaa atcttttgac ggatgaagaa
    41281 ctagcatgtg atcaagacga tttgggaaat tttgtgagta tttgggatca tgtctatctt
    41341 atccatttat cgaggaagtc caaaaaacct gaatatatct atgtcatcga agatggcttg
    41401 attgatgcgc tagaagagta tgacagagat aacttgattg atatctctta ttacggatca
    41461 ggtaagaaat acattgctga aatggaggca gaatttgatg agtgaaaaca aagggacaac
    41521 gacttttgaa aaacttttta gtcgcaagtt aaataaaata ctcaagaaaa aaggaaattt
    41581 tgattattta tcttgggctc acgcatggga gattatgaaa aagaatgatc cacaggcaac
    41641 ggtaactatt aatgagtata aacactacag agttgtttct ggaactcatc aagactttct
    41701 tgttgaggaa tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt
    41761 aacggttaaa ggacacacgg aaaccgaatt atttcctgtt ttagattatc gaaaccaacc
    41821 agttgttaaa ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct ttgtgaaagc
    41881 attggctcta cacggactgg gattatatgt atttcaaggg gaagatattc caacaccacc
    41941 tagaatcgat acgaagaaat taaacatgct agagacgatt ctagaagctt tcaatgagca
    42001 gatgggtaaa gatatgacca aaaccttaat cgaatatgtt aatgagcaga cagataaatt
    42061 agggctctta gctgataacg ttgaaactat tgaacagtta agctatgagc aatgtgcctt
    42121 gatggagcga gcaatagcag ctaagagaaa agaattagat aagaagtgat atgagtgttt
    42181 aaaccattaa tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa agacataggt
    42241 gcaaccatca atgaggaagt gaacattgat cgactaaaga cgatgtatga cggctacgat
    42301 ggtgatcgag ttattgaaat tcgttttatt gatcctagac gtttcacagt acagcaacga
    42361 aacttcatct atgcactcat aggcgatatt ttcatcgata caggcatgcc aacggacttc
    42421 tggaaggaat tcttttactt ccgctttgaa ggtgtcacag ggcgcaaaat aagcctcaaa
    42481 gatgaatcga atacgactgt gagtgatgct aacgtcttag caaatatcat cttagatttc
    42541 atctttgaac atcatattcc tttcaaagaa ggctatgaga ttttaccagc gaatcaagaa
    42601 tattacttct acaaatgcat cacaaaaaga gtctgttgca tctgtggcaa aacaggagct
    42661 gatatcgatc actttgacaa agcgctagga agacgaaagc gcaaagaagt tgatcattca
    42721 gagtacacat ttgcagcact ctgcagaatc catcacacgg agaaacacaa gataggtgtg
    42781 atcaatttca aaaataaata tcaaatcaaa gggatcaagt taaaccagga aacaattaag
    42841 aaacttagaa taggaggata aaaaatattg gctgacaac
//
LOCUS       DADXTH010000014        42521 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.014, whole genome shotgun sequence.
ACCESSION   DADXTH010000014 DADXTH010000000
VERSION     DADXTH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.014"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(39..1328)
                     /locus_tag="IWI97_000734"
     CDS             complement(39..1328)
                     /locus_tag="IWI97_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2462259.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(1355..1657)
                     /locus_tag="IWI97_000735"
     CDS             complement(1355..1657)
                     /locus_tag="IWI97_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2462260.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(1682..3775)
                     /locus_tag="IWI97_000736"
     CDS             complement(1682..3775)
                     /locus_tag="IWI97_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBC2462261.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(4264..5028)
                     /locus_tag="IWI97_000737"
     CDS             complement(4264..5028)
                     /locus_tag="IWI97_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBC2462262.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(5139..6008)
                     /gene="mdcB"
                     /locus_tag="IWI97_000738"
     CDS             complement(5139..6008)
                     /gene="mdcB"
                     /locus_tag="IWI97_000738"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBC2462263.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(6033..7109)
                     /locus_tag="IWI97_000739"
     CDS             complement(6033..7109)
                     /locus_tag="IWI97_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBC2462264.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(7133..7726)
                     /locus_tag="IWI97_000740"
     CDS             complement(7133..7726)
                     /locus_tag="IWI97_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBC2462265.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(7730..9406)
                     /gene="mdcD"
                     /locus_tag="IWI97_000741"
     CDS             complement(7730..9406)
                     /gene="mdcD"
                     /locus_tag="IWI97_000741"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBC2462266.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(9396..9695)
                     /locus_tag="IWI97_000742"
     CDS             complement(9396..9695)
                     /locus_tag="IWI97_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBC2462267.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(9797..11443)
                     /gene="mdcA"
                     /locus_tag="IWI97_000743"
     CDS             complement(9797..11443)
                     /gene="mdcA"
                     /locus_tag="IWI97_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBC2462268.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(11450..12361)
                     /locus_tag="IWI97_000744"
     CDS             complement(11450..12361)
                     /locus_tag="IWI97_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBC2462269.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12481..13338
                     /locus_tag="IWI97_000745"
     CDS             12481..13338
                     /locus_tag="IWI97_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2462270.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(13525..13887)
                     /locus_tag="IWI97_000746"
     CDS             complement(13525..13887)
                     /locus_tag="IWI97_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBC2462271.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(14076..14258)
                     /locus_tag="IWI97_000747"
     CDS             complement(14076..14258)
                     /locus_tag="IWI97_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462272.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            14233..15672
                     /locus_tag="IWI97_000748"
     CDS             14233..15672
                     /locus_tag="IWI97_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462273.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(15679..18231)
                     /locus_tag="IWI97_000749"
     CDS             complement(15679..18231)
                     /locus_tag="IWI97_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBC2462274.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(18345..19676)
                     /locus_tag="IWI97_000750"
     CDS             complement(18345..19676)
                     /locus_tag="IWI97_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2462275.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(19642..21120)
                     /locus_tag="IWI97_000751"
     CDS             complement(19642..21120)
                     /locus_tag="IWI97_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2462276.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(21425..23260)
                     /gene="tdc"
                     /locus_tag="IWI97_000752"
     CDS             complement(21425..23260)
                     /gene="tdc"
                     /locus_tag="IWI97_000752"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBC2462277.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            23730..25157
                     /locus_tag="IWI97_000753"
     CDS             23730..25157
                     /locus_tag="IWI97_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462278.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(25249..25614)
                     /locus_tag="IWI97_000754"
     CDS             complement(25249..25614)
                     /locus_tag="IWI97_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBC2462279.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(25611..25976)
                     /locus_tag="IWI97_000755"
     CDS             complement(25611..25976)
                     /locus_tag="IWI97_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBC2462280.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(26168..26416)
                     /locus_tag="IWI97_000756"
     CDS             complement(26168..26416)
                     /locus_tag="IWI97_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462281.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            26415..26543
                     /locus_tag="IWI97_000757"
     CDS             26415..26543
                     /locus_tag="IWI97_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBC2462282.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            26600..28294
                     /locus_tag="IWI97_000758"
     CDS             26600..28294
                     /locus_tag="IWI97_000758"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBC2462283.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            28298..28417
                     /locus_tag="IWI97_000759"
                     /pseudo
     CDS             28298..28417
                     /locus_tag="IWI97_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            29063..30196
                     /locus_tag="IWI97_000760"
     CDS             29063..30196
                     /locus_tag="IWI97_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBC2462284.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            30227..30547
                     /locus_tag="IWI97_000761"
     CDS             30227..30547
                     /locus_tag="IWI97_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBC2462285.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(30576..31040)
                     /locus_tag="IWI97_000762"
     CDS             complement(30576..31040)
                     /locus_tag="IWI97_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2462286.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(31171..32424)
                     /locus_tag="IWI97_000763"
     CDS             complement(31171..32424)
                     /locus_tag="IWI97_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2462287.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(32453..32770)
                     /locus_tag="IWI97_000764"
     CDS             complement(32453..32770)
                     /locus_tag="IWI97_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBC2462288.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(32796..33116)
                     /locus_tag="IWI97_000765"
     CDS             complement(32796..33116)
                     /locus_tag="IWI97_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2462289.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(33580..34995)
                     /locus_tag="IWI97_000766"
     CDS             complement(33580..34995)
                     /locus_tag="IWI97_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2462290.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(35012..36328)
                     /locus_tag="IWI97_000767"
     CDS             complement(35012..36328)
                     /locus_tag="IWI97_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2462291.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(36478..37176)
                     /locus_tag="IWI97_000768"
     CDS             complement(36478..37176)
                     /locus_tag="IWI97_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBC2462292.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(37465..38420)
                     /locus_tag="IWI97_000769"
                     /pseudo
     CDS             complement(37465..38420)
                     /locus_tag="IWI97_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(38533..39366)
                     /gene="msrA"
                     /locus_tag="IWI97_000770"
     CDS             complement(38533..39366)
                     /gene="msrA"
                     /locus_tag="IWI97_000770"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBC2462293.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(39541..41169)
                     /locus_tag="IWI97_000771"
     CDS             complement(39541..41169)
                     /locus_tag="IWI97_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462294.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(41156..42490)
                     /locus_tag="IWI97_000772"
     CDS             complement(41156..42490)
                     /locus_tag="IWI97_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462295.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12942 a         8771 c         7203 g        13605 t
ORIGIN      
        1 ttcttaaaaa atccaaacat cattttccct actttcaatt aaaatgcttt caaaactggg
       61 cttaagatcc aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca
      121 ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag
      181 aaaccatgag caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat
      241 atccctgctg tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag
      301 ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct
      361 aaaatgacag catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca
      421 tcaggaacta atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga
      481 cgtacaccag ctagcaatac atacacaccc actacgaatt ggatggcttg taaaaaggca
      541 aacatgatat agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa
      601 acagtgatga tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga
      661 aattcgaaat gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca
      721 ccaaaaagct tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa
      781 gcaatatcat ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca
      841 atggcgaaac aagctccgat aaaatcaccg accaagatca atacgatccc tcgcagaccg
      901 gcaaaatagc caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa
      961 aaaatgtact tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag
     1021 aacgtcagtg caatatctct acctaaaccc aaatcattca ttgcttgacc attgatagat
     1081 tcaatagaag ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa
     1141 gttaaacttc caacgatgat gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc
     1201 tttaacgtgc cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata
     1261 gcaatcaatg caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata
     1321 acgcccatgt ttttaccctc ttttcttttt gtatttagat aactccctta tcttgcagaa
     1381 ctggggtgat tttttcttca atttcatttt ttgaaacaat atttttcaaa tagatcatcg
     1441 ccgttttagg atcaactgcg aacttttcga actgcgaacg aaaattttct gctgtgatga
     1501 tcacatctgg tttcatggaa actgcggaag aaatatcaca atggtccagt ttggcttcta
     1561 ctcctaattt gttcaaaaca tcctcagcag acatctgtgc tgcaaaacta cttcctaaac
     1621 ctgcaccgca tacgaataaa atgttcaatt tgttcatctt tgttctcccc cgtatcattt
     1681 attatttctc aactactgaa aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa
     1741 tcaatcgatc caacttctct tcgttgttga tcaattcaat tagttctttc atgatgttca
     1801 gatgcgagta ggagtctact gctgccaaac aaaaaataat ctttactgga tccatttcag
     1861 gattaccgaa atcgattggt tgctcaatcg tagctacgct gactcctaat ttattgaccc
     1921 catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc aatcacgata tacggtccgt
     1981 aatcattcac tgcacgaatc atcgcttcga catagcgctc ttcgacaatg ttttctttaa
     2041 ccaatggtct agcagctagt tcaatcgatt cctcccaagt agtggctttt tctttgatca
     2101 tgatattgct gtccgtcaaa atatctttta acataggctg gatctccctt ttgttgattt
     2161 ctaaattgtt tgtttcgaat aatttttcta acttgccata gatgattcga tctactcggc
     2221 caccactttc tttcatgaca tctattattt gagtgaataa ctccgtcgat tcatgactgt
     2281 taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa gtcttctata accaagatat
     2341 tcgtctcagt taaaatcgga tcgacaacta aaagcggctt atttcgataa ttcaattgaa
     2401 aggtgctgaa aaccagattg acatccaatt tatctactaa ttcgatttct tttgaactta
     2461 aaactgcagt aatctcgatc tgaggaaact tttctttcag attctctgct aacagatttc
     2521 cagtagccat cccatggtta cagacaacga ctgatttata aacataattc aagtctcgct
     2581 tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa agctatctcg tcctccagta
     2641 tcttgccacc aattacttct tcgatcgtag gaacaaaatg agtgactgcg cggtagattg
     2701 ggaaataatt tgttcggatg ttttctttca atggattcat gacttggata tgattcttga
     2761 ttcttacaat cagtgcagcc atgtgcttat agagattttc gaataaagct tcttccttta
     2821 gatggaaagg tacgtgtgtc tcgttctcta caaattggat caattggatc gttagtaatt
     2881 gagcttgtac ccactcgatt gaattggaga tatctctagc attgaacgtc tcaatagtga
     2941 aacgaagata atttctttct acttgcggga cattgtccaa ttgaaattcc tcgatcatgt
     3001 ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt atttttccaa ttaatggcag
     3061 agataaattc atgtttcctg attcgctgga tccagatcaa tgtaaaaagc aggatttgtt
     3121 ttttataaat atcatcttct tgtttcaaca acttattgga atagatctcc tgtatttttt
     3181 tgatttccac caaaggaaaa taatggagaa agattttttg agccggtgtt aattgtgcat
     3241 cttgattcat aaagaagtcg gtcattccca gattctgatt gataaaatca tagatcatcg
     3301 tacggataga tcgttctgaa cctttataaa ccagtccttg ctttccataa ctgacgatct
     3361 ctatcccata actgaccagt gttgctcgta accgtctcat atcttcatct aatgtacttt
     3421 tagagatttg aaattcttct tcttttttgt ttagatggat cgggattcgg ctgtaagcaa
     3481 cggctaaaac tagatcaaaa atacgttcgc gccgattcaa aaagccgtct ttcacatctt
     3541 ttatttcagt ttctaatcga gcttgctttt ccttttccag cttcaacaaa aaacctttgt
     3601 tgcgaattgt acggatctcc ggaagctttt tactgctcaa ccagatattc gcttcaagaa
     3661 cttcctgacg gatcgtccgc tgactgacat tgaactcact tgctagatgt tttgctttta
     3721 ctggatattg ggctgtcatg attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa
     3781 tgatctcctt ctgatttcaa gtatatggtt ccgcttacat aataaaaagt agatatttct
     3841 gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa aataagccat atgctttttc
     3901 atctcctcaa cgaaaaagac atacgcctat tattacaaaa attttgccca tctatacaaa
     3961 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacaactaaa ctaaaatagt
     4021 gcttcctatt tttcgtttgg ttgttcttat tatcgcagat gcaagctttt aaacaccatt
     4081 tagtggagag gttgtgacag agatgttcag ccttgagcga taagaagcga ttttgaaaaa
     4141 tcgcttccca tattttacaa aatcgtgcct tattgccgat aggctatctc tggcacaccg
     4201 tttattcaga aaaaggagct gcgacaaggc ttttgtcaca actccgttca taaatatttc
     4261 ctgtcaatcc agcataatcg gctgaataaa atcggcttgc catctttttt ctttctcaga
     4321 aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa
     4381 tcgcccatct tttcgatcga ctgaataatc acagcatctg aaatcaattc ggatggcaaa
     4441 actattttcg tggatctgtc gataatttcc tacttcctga atagttattt ttttagtatg
     4501 atttcttaca ccgtctgctt tgtttttttg cagaactttc tgatgttcgg caaacagctt
     4561 gtctgtataa cagcttctgg ctttttcagg agtttcttct tcccaagcgg attggatctt
     4621 catgaatatc ttttggattt ccagaaactg tgctttcttt tctttagccg ttcctgacat
     4681 gcgggacaat atctccgctt tcagcctttt cttttgtctt ttattcttga cgatagaaaa
     4741 agcagaaacc cctcctactg acaaaacagc taatgcaaga aatcccgttt gctcataact
     4801 agaaggacct gtacctggac cataataccc ataagaatct ctgccgtaag aacgactgcc
     4861 aaaataactg cccccatatg aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct
     4921 agatgtacct ccaccatgac cacccgcgac agcgaatcct tgggtcggca gagcaaacaa
     4981 acataggccc actaacaaaa tgattgctaa ataattgatt tttttcattg tcttcctcgt
     5041 tatcttttat tacaaaaaac tatttttatg atagccttgc taaaaggaag agtcaataac
     5101 cagatgcata aactgttttt ttagcaattt tgtcttattt agtcattgag taacatcgga
     5161 caaatgtatg tgtcggcagt ccataattcc tctatcttca gcatgaacaa gcttgcagac
     5221 aagagatctg cgcttcctcc aggagagatt tggttttcct gacaaaagtg caacagtgct
     5281 gtaaatggac gatttggtaa agcactttga ctagcagcat gagcaagttt ttgcatttcc
     5341 actaacgttt tctgatcact gcgatgcaat acgcatgtgt catctagcga actcatcaaa
     5401 actaacaaca tatgcatttg tttctgtaca tatgaatcat tatctagctc cttagctgct
     5461 tgaattgcaa gcctgatatg cggataccct aatatcgctt cttcatatgc accatttacg
     5521 ccatattttt gcttcaccct atgaccatga gtggtttttt tatgacgata ttttgtatca
     5581 ggaaaagcag caagcttgct gacatcatca agtattttcg ataaagaaaa aggctgttcc
     5641 atactgattt tttgtgcaat gacactgatc aatagcccca ttgcccaaat cgctccttta
     5701 tgggtattga tgccatttgt agcaatcagc attgcctttt ccgcttttcg cccaatctca
     5761 gcaattcttt ctcttaactc ctgatcaata gcatgatgcc atgctatttg agccatttct
     5821 tcaaaaaaag gagttagcgt cttagcagac ttaagaaata aagcatagtc catatcggaa
     5881 tgtgcacccg catccagtgc atccactaat ccaggttttg gacttaatgt tacttctgcg
     5941 atcaatgctt cctctaccaa cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca
     6001 tttatcatga cactcactcc ttcttcctac tattacttgc tgaaaccttt tttgatagct
     6061 acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac aacttgccgt cattagataa
     6121 gctacaagaa tcatgactcc tgaaggtaac tgcagccaac tatctttaaa caataataac
     6181 agtacttgga aaacaaatac attggctaaa tacgtacgat aagcataaac agacaaccat
     6241 ttgaacaatg gcaattttgg cgaatcgttt acaatcattt tggaagcgcc tatgaagaca
     6301 gcaaagatga tgactaaact gtataaactt tggatcgtat tcaagtaagg tgcattcgca
     6361 aagctcaagt ctcctggatg tgcgagcaat gaacggacag aaaagaacgc gataactaag
     6421 gcaacaggta agaaaagata acgtactctg tccaaatatt gatagacttt ttcgtgataa
     6481 acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa accgatctaa taagtaccag
     6541 ctgtcatgat aaggcccagt caatacttgt gtttgataga acagatacca tgcgacatat
     6601 aaaatcgttg aaccaatcaa tactggccat acatattttt tattagcaaa tactttgtaa
     6661 ccgagccaaa caaaaaacgg catgaacaat tggatctgca gcatcataac ggtataccat
     6721 aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa agcttgcagt atccgtatac
     6781 ggagtttgtt gttggactgc tggaaatagc caaagataag ccgtcgtcca tagaagatac
     6841 ggaacgacta gttcaaaaaa cttttctttc aaaaattccc aataagacaa atgctgactt
     6901 gtttttacca tattataaac aattgcaaaa ataaacattg gtgctgtgta tttaacgatg
     6961 acgtaaaagg taccaagaaa agctgtattt gttgcgctgt ggctgtttgc caaagcaaag
     7021 ctcaatatcg tttgcatcat aacagccgtt acagctgata ctttctggaa gtcacttacg
     7081 ttgattttga tttttttgtt ttcaaacatc tctttctctc tctttttctt tattaccaaa
     7141 gttctttggt taatttaggt ccttcgactg ttttcaatag aatttttttc tctggctctc
     7201 tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac tacttgggta tcgaattgct
     7261 caaaaaaagc aggatgcgat tgaattacct gaattggcaa ttcaatcggt gaatttgcgt
     7321 acagcagcaa atcaaaatca ctggtttctt ttactgccgg aatcccagtc gataattcaa
     7381 atcctaagct gccgccgatt ccccatttac atcctgataa tatgtttcta gcagcatgaa
     7441 attgctgata aacaggataa tccatgattt tctcttgtct atacgattct tgttcgatga
     7501 tgtcccacgg atgaatgact tgtttgacgg catgttttga gatttctgtt gaccatctct
     7561 gattgcgggc aggtccacgt aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac
     7621 gacgaactgt tccgaacatc ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt
     7681 ctatttcatg tttatcaaaa aggacaatat catgtgcttg aatcattttt tatgcatccc
     7741 attcttcgtc cattttggca cggactttat ttgtttcgac acgtccgaat gttttggctg
     7801 tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg
     7861 cttcattgat tgcttcgatg actttgctga cattttgttc tgaagcagct tggtcggtga
     7921 tatcttctag gaaatgatag atcgctccta gttttgtata attattgata tcgtaagcga
     7981 tagctggaac tttttcagca gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg
     8041 cagcactttc tttagacatc gcttggacgg taatttcttt attgtttaaa gcaatcaagc
     8101 ggcttgattg taatccatgt gctaaaaatg cacctgaaat agcatttcct gcaatcaatc
     8161 caataactgg atgtccagct tgacggagtt ttgcataagc agcagcacta ttagccaaag
     8221 ccacatggat accgatcaat tcttcgttat agccgtatgc ttggcttggt acatcgatca
     8281 caacgatgat tggccgtttc acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa
     8341 tttgtcccat gcggaaacct tcaactaaac caacttcacc gtttcgtaca cggtacatcg
     8401 ggtttttagg atcaggtacg attgatgtca cgatggcttc ttctatgaat ttacttgatt
     8461 taccataata cacagtacta atagaagaag ttggattttt catgccagtt agtgccgcaa
     8521 accaatcgta accaacactt gctgcagctg gttcatttcc ttctgctact tctggtaagt
     8581 caattgattt tgcttgatgg ttagcaattg cttcattgta atcgtcaatg gccattggtt
     8641 ttgaaagatc taaagaatca agtaatgata aataaaatgc tgagctttca ctgcgatgcg
     8701 tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt gattgcttct acactatcag
     8761 tcactaattc atcaatgatt tttgattcta cccgttgacg agatcctaat gtattccaaa
     8821 tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc ttcttgttca ataacttctg
     8881 gtccattcaa accgactctc gctttttttg ttgcgattaa ataactcatt aatgctgaag
     8941 taatcgacat accgccaaat gatcctacac gtcctggaat caaaccgatg actggtacat
     9001 attgttttag agcaatgatt acatttccga tttctgaaat agataacagt ccgtagtttg
     9061 cttcttgcaa acgaacacca cctgtatcta gtacaatgac tggaaagatc tcatttcctt
     9121 ttttattttc ttctaacaca tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac
     9181 cgataccgcc accttggaat tttccttcca tagcgattac tactgcttgt ttgccattga
     9241 tcgtgccacg gctgacaacg atcccgtcat cactttcagg gacgattcct tgtgctggta
     9301 aacttggcgc gatgatttga tcgaatggat caatcaattc acgtccattg tcaagtagag
     9361 cgttcactcg ttctctggcg tttaattcta caaaactatt tttcatctga aagttcctcc
     9421 attgcttgcg tcaatcgtag gtacacaact cctggtgtag caccgaagtc attgatcacg
     9481 atatctgctg aaattggata acgatcaaaa aatctcgtta atacattgtt ccatacttcg
     9541 ccgaagccat cgcttcctgt acaaattttt acagtagatt ctgttttttc tgttggaaac
     9601 atcaagattt ccaaatctcc tgaaccgacc actcctacat ggattttctt attgatcggc
     9661 tgtttcgttg gatatttata agctaattgt tccatggatc tctccccttt ttgtaaaaca
     9721 gagcatacac gttctgctta ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag
     9781 gtttttctcg ccaattttat gaccaagttc tgaatttcat tggtggttca tacaggccat
     9841 ctgaccattc gatcaaatca tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac
     9901 tacgtttgat gcctagatct tctggtaatt tcacgatgcc ggctgcacgc aagctatcta
     9961 gtgattcttt cgtgctagtc attccaagag gtgtaacacc gccaattgct gcaattgctg
    10021 cttgtcgttc ttcttttgtt tttgctttgt acaaataagc aattccttct tctgtcacga
    10081 tatgtgtagt atcttcgcta taaatcatca ctggtgcatt ggctaagcct gcttgttttt
    10141 gaacagctag tgcatctaat tcttcgacaa agactggttt tttatttgaa ccatatgttt
    10201 caaccatttg aacaactaat ttttggcctt tccctaatgg atcatcattg tcgcgaagtg
    10261 attgccatgc tggtgtagaa tgacgacgac cgccaggatt atgtcccatg ttaggcgcac
    10321 cgccaaaacc agataaacgt ccttttgtta cggtagaaga gtttccttcg taatctaatt
    10381 gcaaggttga accgataaac atatcgatgg catattgtcc tgcagcttgt gataatgttc
    10441 ggttactgcg catcgtgccg tctttaccga caaaaaagac atctgggcga gcagcaatat
    10501 atttttccat accaacttcg ccaccaaaac tatggatgct ttcaacccaa cctgattcga
    10561 tggctggaat catcgttgga tgtgggttca atacccagtt cttcgcgatt ttccctttta
    10621 accctagtga ttcaccataa gttggcagta acaattcgat agctgctgta ttgaatccaa
    10681 taccgtgatt caaagattgt acgccgtgtt ctgcgtagat tcctttgatt gccatcattg
    10741 ccattagaat ttgcaattct gtgatgtttt gaggatctcg tgtgaataaa gcttctaatt
    10801 gataaggttt atctgctttc acgacaacgt ctacccagtc accagggata tctacacgtg
    10861 gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat accatctttg aaagctgcag
    10921 cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt gtacaagttg ccgtctttat
    10981 ctgctttatc tgcagctact aaaacaacat ttggaatcaa atcgatgaat aagcgtccat
    11041 aaagttccaa ataagtatgg atatcaccta atttcattgt gccgtcttca atcatttggg
    11101 cgacacgcaa gctttgtgca ccagcataag agaaatcgat cttgcttgcg attccttttt
    11161 caaaaatatt caaatgttct ggtcgtgaga tactagacat gatcaaatgc agatcattca
    11221 ctttttctgc atctactttt tcaagacttt ctgaaagaaa acttgcttgt ttttgattgt
    11281 cgccttctaa tacaactttg tcgccgggag caatcagttt ttccattact tctacgattt
    11341 tttcagtttc tacatacttg ccattgagca agttgctgat tttttccaat cgctgcgctt
    11401 ttttttcgcg tttagaagcc cagttgcggt ttggtttttc cataattagt catctccatt
    11461 tttgatatag aaacgtggcg tcatcaatac catgttggtc tagattgatc gtacggatcc
    11521 ctttatctac accaaatttt gtatgaatca gctttgtcaa agtattgccg gggggaaatt
    11581 caatcgtcag atccattcct aattcttttg ccaagtccat catttggttc cactgcacag
    11641 ggaaagctag gttttccaac aaatctttac gaatctcttc tgcgtcctga gtggctcttc
    11701 catccgtatt ttttatatac aaacatctag catcagatag atggacagac tgcatatacg
    11761 gcttgaattt cttgaccgta tcattcatca agagacagtg agatggcaca gggactttca
    11821 gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc caacgtaacg gataaacctt
    11881 cgatactccc agataacgta tgttgcatcg gacaattttc atttgacaga taaactggat
    11941 ggtcaggatc aaatgttttt gctacaattt cctcagcttc gcttctcgtc acacctacta
    12001 ctacgcccat tgcataaccg cttggataag cttctttcat taagactgcg cggtgataga
    12061 ctagttccgc tgcattctca aatctcaaag atcgacaagc aacagctgca caaaaagcac
    12121 caatcgaatg tcctgctgca atatcaggct gaataccgtt tttttccatt tcatctaaat
    12181 aaaatactgc ttttgtcagt aatgctagct ggataaaaac cgtatcttga tagttttctt
    12241 ctttatcaac taaccgatac cccgtcacat tttctaaacg ttccatgtac atttcaggga
    12301 tatccgctaa catccctaac ttctgactgc cttgtccagg aaataagaaa cctgtactca
    12361 tgagcctcgc ctcctcagca cttacttttt ccactgctat cttagtcaat ttctgctata
    12421 ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg tatggatggg atggataatt
    12481 atgaaattgc ttcatctgca gtattttaaa aaagtattcg aaacaaaaaa tgttacccaa
    12541 gcagccaaag aattatttat ttctcaacct gccttgagcc gtgcgatcaa acatttggaa
    12601 aacgaattag gtgtcccttt gttttaccat agcggcagaa acattgagac gaccgtttat
    12661 gcagaagaat tttatccata tgcagtcaag acattagaca cattagaaga aggaatcaaa
    12721 ttattgtctt cggtcaatga gaagatcgtt acttccgtca tattgtattt agaggtagcc
    12781 tctgtttcta ttccgaactt ggtccgaatt tttatggaaa agcatcctga tatccaatta
    12841 acgatcatgc aacatcattt accagatgat attaccgatc ctgtactgta catcacttcg
    12901 gagatgaagc ctggcatgac caatattcct atcatgaaag aacctgttta tgtcgccatt
    12961 cctaagaaac atcctcttgc taaaaaagaa catctgcaat tagaagatat ccaacgcacc
    13021 cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata cactggatgc agcacttgaa
    13081 gcgaaaagta tcgaattgac gatcagttcg acgacagatg atcctgcgac attacgttct
    13141 attgtcaagc aaggattagc tgttagcttt ttcccaaaag tttcttggtc ttacgataaa
    13201 aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct tgacacgaac aatttattta
    13261 tcttctagct tcacagaaga tcatcctttg atcaaaatga ttgcaaaaac gatgcgggaa
    13321 ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa ttcttgtcgc agctcttttt
    13381 tcgaacaaac ggtgttccag aagtaatttc ttagaaaata agccgaaatt tcccaaaaat
    13441 ttgagaagca attttcgtaa attccttctt atttcttgaa gctaaacatt tctgtcacaa
    13501 cctcttctct attgtcctag acattcattg cgttgattta aaacttccat caatcaaatc
    13561 tgctagttca tctagctttt ctattttacg tagatcaata gagatccaat gttctttatt
    13621 catgtgatag gctggaaaat acccctcttt tcctcgcaaa attccgccaa gttcaggatc
    13681 gaccttcaaa tcaagaatat tgatgatcct atggtcactt gcatcatcat agattttttc
    13741 ttctggaaca gacatcagga ccccaaacca ttttttgctt ttttgatgtc tgaatacgca
    13801 ataatcagga aattttttaa aaatatattc tggagacaaa ttgtatgttt cattgatgtg
    13861 atcgattagt tcttttttcg ttagcatctg cttccatctc tttcttattg attcgcttta
    13921 ttcaaagata ccacgtaaga atagaaaaat gaatacaagt agttgctaat caaatacttc
    13981 gatatttttc ttttttatca actcttaaat gttcaatccg cctgtgttgg tcttgctacc
    14041 atctactatt aaaatgttta ttattgtttg cccacctatt ttttgatttt agtggtatca
    14101 tttttcaagt tcatgatgaa attcttatca tcagtaaatg ctccagaaaa ataaacaatg
    14161 attgaaaaat cagttgaaac ttgaccaccg tttattttct ccgtattctt agaatagaag
    14221 ggagtctgat tgatgtattc actaatgaaa aaaatcatta ccgaaaaaga tattcgacgt
    14281 catgtttctt tagtagaaca attgctgaat catacaaaga taaccgtaaa tgaattagct
    14341 gaaatcatag ggacaaccga acgaacgatt ttttctgacc ttcaatctat tcgctctcag
    14401 ctgcccgaag gctgggacat catcagcgat caagcaggaa tcagtttgca aaaccagcaa
    14461 aatttattaa caaatgacct ttgggagatt tttttcaaac aatcagtcag tgtcgaactg
    14521 ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg attttctagc cgaccatggg
    14581 ctttcttatg ggacattgaa aagacatgtg actaaaatca atcagcgttt agcaagttac
    14641 gacttgcaga tcgatttgac gaaatataca gcttgtattc ttggaaaaga gcgtgcgatt
    14701 cgtacttttt atcatcgatt gctgattcct ttcactcata acaactattt tttcgaggat
    14761 tattcgatcc atgaatccca ttattttcaa tttttaagaa acctcagtca aactgaacta
    14821 gcggttgaaa cagaagagat tttcggaaca tgttggttct ttatcaatac gatccgtata
    14881 aaagcgaatt gccgattaga ttcttctatc cataccaaca gcacactctc ctccctgtat
    14941 gactctgcat tgaaaaaatt gtatttaacg gaaggtattt atcttaaaga cactgagctt
    15001 tcttttgctt ctttctgctt tctggaaagt tggaattaca acaataacta tggacaagaa
    15061 accgctcgct gcctgcatca ttctccattt ttagaggtcc tcgaaacgtt tgtagaggaa
    15121 ctagcttcag agctgtctct tgatcaacta aaaaaaacat ctctgactga taacttggct
    15181 ctcctcatat taaaatatca tgaatcgccg atattgattg aagcattgga ccgtcaatac
    15241 catcgttttt tagaaacata tgatcagcaa tatgcacact tgtatcaaca gaaagagaat
    15301 ttactgaacg atctaaaaaa agaaatagaa atcaatgaac caaattactt tctccaactc
    15361 ttgtctctac tgatccaaca aacgattttt tctatcaaac cgaatttgtt caatgtctat
    15421 tttattttcc aaggagaacc ttcatggaag gcgttcttgc agcaggaatt aaaagattac
    15481 ttaggaaaac gtgtctgctt cttacctatc gaactaaccg atctttccac tatttctttt
    15541 gaaaaaacag atatcttgat ttccaatttc cctctagatt ctctagatat ccctattgta
    15601 tacatttctt ctattccaac gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa
    15661 acttttttat agtaacggct agcgactgta ttctctagct gttttttctt ttttgcgaaa
    15721 cagtttgttc atgatccctt tttctatttc tacaactaag aatagaagaa tcacatttgc
    15781 tccaatcaat aactgttgtg tcatcgttaa acttactgta ccgatcagct gctggccgac
    15841 tggtagaaaa ataaccatcc cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc
    15901 tttgttttgc aaaagacctt ggttcaatga cggcttggac aactctctgc aattgatcat
    15961 gtaaacagct tgagctaaga cgatattttg cagaagaatg gtttgctgta agctttgccc
    16021 ttcaaactgc atagccaata aataggcagg aaccatgatc agtagggaga catagatgat
    16081 ccgaaacaaa tgataaccag ataggattcc ttgctttgga tctcttggtg ggcgcttcat
    16141 gatttctgga tctggcgact cgaaccctag tgcataagat aaagtgattg tcgttaccat
    16201 attgacccat aaaatctgta ctggggacaa aggtagcggg cgattcagca taagtgccca
    16261 aatcaagatc aatccttgag ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa
    16321 attatccaaa atccgccggc cctcttttac ggcttttgca atcgtatgga aattatcatc
    16381 tcctaaaacc atatcagctg cctgtttgct tacttcacta cctttgattc ccatagcaat
    16441 tcctacatcg gatcttttca aagcaggtgc atcatttacg ccgtctccgg tcattccgac
    16501 gatttctcca tttttctgca aagctttaac aattcgcagt ttatgttctg gtgtcgttcg
    16561 agcaaagaca tcgactattg gcacttgctg aatcaactct tcatcagaca taagatccaa
    16621 atcgatccct tctaaaactt tggcagtatg ttttaagcca atttgttccg caatcgcttt
    16681 tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag atccctgctt gttggcattc
    16741 ctttacagct ttgactgcac tttcttttgg cggatcgatc aatccggcga tccctgcaaa
    16801 agttaattgg gttaaagtct catgcgtgag ttcctgtttc gaatctactt ttttataagc
    16861 aaatcctaaa acacgctgac cttttctcgc aaattctgca gctgtctgac tccatgcttc
    16921 ttgttctgca gaagaaagag ttgatttagc aaataatact tctggtgcac ctttgacgta
    16981 aatcaccgct cctttttcct ccgcatgcat cgttgccata tatttgtatt cagaactgaa
    17041 cggaatcttt gcttctattt ttttcaaagg tagattggct gtttctgtgt gtcgaatcag
    17101 cgcaacttct gtcggattgc cacttaaatt ttctgttttc tgttcttctt ttgtttttaa
    17161 ttcctgacag tttttcataa tggacaacac gcaggcttct tcttttgcag ctacatccac
    17221 aactgtcatt tcattttttg tcaatgttcc tgtcttatct gaacagatca ctgtcataga
    17281 acctaatgtt tctaccgaag gaagtccttt gatgatcgcc ttttcttttg ccatttcatg
    17341 tactccaacg gatagaatca tggtcaaaac agctggcaat ccttcgggta ccattgacac
    17401 caccaaagca atcgtagccg aaagaaggat atgccaatcc ataccatatc gaaatgtcgt
    17461 aaaaataact aagaataggc tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg
    17521 gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc gattgcaatg cttgattgat
    17581 tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag aaccagattg
    17641 tacaagcgtt cctgaaaaac ccatattttt ctggtcgccg atcgattctt cttctgtcaa
    17701 aacggcatga cttttttcag atgcttctga ttctcccgtc aaaatcgatt cttccactaa
    17761 taaatcatgt gcttcgatta atcggatatc tgctggaacg acatctccgg cacgtaagac
    17821 aatgacatct ccttttacca gttctttcgc agggattttt tcttgcatcc cgtttgatag
    17881 tactatcgct tcttgtccca tcagctgctt caaaccattc aatgattctt ccgcttttct
    17941 ttcttgccag taactaacga gtccattgac gatcactacg aataaaataa tacttccttc
    18001 aatatattca ctagaagcaa atttcaaaaa agaggcaatg atcaacacga tcatcagcag
    18061 atcagtaaaa tgctttgcta tttttttcca tcgttttgtc tgctgccttg cttttatttc
    18121 attatatcct tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc cttctgttga
    18181 tgtttgatag tttttcaata catcttctac cgtcaatctg taccagttca tcctatcccc
    18241 tcctgtttaa ttgatactgt tattatacgc agggaagttt tttcaggcgt atcgattatt
    18301 tcaggggggt ctgaaataat tcgttttgca aaatgtatct gggcctaact gatatttcta
    18361 ttgcttattc ttttatatac aaaatttaag aatacaaacc agataaccaa taaaaaagga
    18421 ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac
    18481 aaagcaagat ctttatatct ttgtgtagtg ttccgtttct tttctttaat aaattgaaca
    18541 tgtgcacaca agatcgaaga ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa
    18601 agctgaaaaa acaatcgact atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat
    18661 aacattcctg taattaaaat accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac
    18721 caatggggca aacttttttc ttttgctgtt tgatataaaa tcctgctagt actgtagact
    18781 gcactatttg ctgaggacaa agaagcaacc aaaatcatta gattgacaat tgctgctccg
    18841 ccttggcaat ggatcacttg cagcatagtc actaaaggac tttcttttaa atctaaacaa
    18901 tgccatggaa caacaaataa aatcgacaaa atcgttccta tatagaaaaa gagaatacgc
    18961 caaggcagac agttgattgc ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg
    19021 gttaagccga tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa tgagactacc
    19081 attccagcaa agccaaacgg tagtaattca tcgatctgtc caaaattttc tttgattccg
    19141 atagactggg tatgaaaatc tgagacactc aatcctatac ccaataagat aaaaaggaaa
    19201 atccccaaga tcttgatgat tgagaaaaaa gattccaatt caccaaacca cttgactgca
    19261 acaaggttga ctgccattaa gatcattagt gcgctacctc cgcttatcaa agctggtaca
    19321 tgtacaaacc agaaatggat atacaatcct attgctgtta attccgcaag tccagctcca
    19381 agccagcaaa gccaataagt ccaattgaca aaaaatgccc actttttgcc taaataatag
    19441 tcaacgaaat cactgataga gttaaatgta ctatttgcta aaaataaatc acccatggcc
    19501 cgcatcaaac cataaatagc caaaccgcat atcaaataaa cgagaataac agctggccct
    19561 gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg ttccaattgc gccacctaat
    19621 gcgattaact ggatatttcg attagttaga gtccgtttca ttgccggttt ttccatcaag
    19681 ttcacctttt tctttttcaa gcagcatggc gacttcctgt tcccaagaag gtttctttat
    19741 tttttcaata cagagtggta ctacaaacaa aatgacactg atggctgtga ctgcaattag
    19801 attgatccat gttccagctt tcatcgtcag tccgacagaa aatccgattg ccgcaaataa
    19861 aacaataacg acagtccaaa ctccaagatt gctctttttc tctttttttc caattctaaa
    19921 tggtcgattc aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac
    19981 atagacgata cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac
    20041 gccaggaatc agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat
    20101 aacggatttg gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc
    20161 accttttcta gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg
    20221 gactgctaca ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc
    20281 aagaatttgg caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt
    20341 tggtacgaca tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa
    20401 cgcggcaaat actccgcgaa tatacgtttt aactggattt tccaatcgag tgatatacac
    20461 agaggacatt tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga
    20521 tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg
    20581 tacgatccca actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac
    20641 gtacaatcct aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt
    20701 caaaaaagtg atgatccaat aaacgactaa aatgacaact aatgtaaatt tgctgttcaa
    20761 accaagcggc acattatcga taatattacc aaacaacgga gcgagtgctg aagcgaccat
    20821 caccatccca ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt
    20881 tcctagagca ttcgataccc aaagttctgg accacctttt tggggaaaca ttccagcaaa
    20941 ttcaccagag atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa
    21001 aatcatcgtc cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc
    21061 gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat
    21121 ctttattcct tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa
    21181 cagaaatgac aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg
    21241 aacgatctta gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt
    21301 tataaaaaag aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt
    21361 gatgaagcaa ataattgtct gttctccata tgtctcttgt tattctttca cacaaaaatc
    21421 agttctattg gatgatttcg tttagtttct tttccattgc tttggcgatt ttttttgtat
    21481 aaaaattgaa aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag
    21541 tgattttctg ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa
    21601 tcggagaatc accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac
    21661 cataaacatc gccatccata taagaagacc gatcgaacat tttttcattt aattcattga
    21721 tagcgttcaa atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag
    21781 gatgattcaa tggatagact tcgattgttt ttcctttaac agtaaaagtc agatgatcga
    21841 gaaattctct gaatctttga gctgcctcga ttgaagcccc aatcaattga ccatatccgg
    21901 tcacattcaa tggcaataca cgatgtgctg tccagactgc ggcggcggtt gctccagctt
    21961 ttgatccttc caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca
    22021 catatggcgc gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc
    22081 cagcagcata agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg
    22141 aaatagctct gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat
    22201 gatgttcttc aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta
    22261 gaaacagtga acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatcc
    22321 cttcatcttt taacctttct cttaattgga cgattttatc tacactgtct acttgacctt
    22381 cttctgtcgt tcctacgaca gcaaccacac ctagaattgg gattttttgt ccagctaatt
    22441 cacgaattgt tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag
    22501 caaccatctg atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt
    22561 ttgtttgagg gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag
    22621 ctgctttgac ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt
    22681 caacagataa attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt
    22741 ccttgacagc taaaggaatc gatttgatgc aacgggcgta ccataatcct tcaagattag
    22801 caatagaacc atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa
    22861 aatcctgtcc tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat
    22921 tatttgggtt ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag
    22981 cattcatttg tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc
    23041 gctgactgac ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag
    23101 tgagcttatc tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc
    23161 caagatgttc atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt
    23221 tgtcgcctat aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt
    23281 gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca
    23341 aaaatgagaa tttttttttt ataacatcaa atccacgtag atttttacaa acaaaaataa
    23401 ataaagtcgg atttttctga taattcaacg caaaaaagcg aacaattttt caataaatca
    23461 gtcttctaaa atagataacc taaaaaaagc gaacaaacaa ataaaatgac tgtccttcat
    23521 tcactaaaca tatttttatt tttttatatt gttaaaaatt cgcaatttat tttttaatag
    23581 aattatctgt tttccaaatt ttataacata aaaaaccttt tttaacggca tttatatgct
    23641 ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt cataatattt cccatattgt
    23701 gcaaaaaaat acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt
    23761 taagacagct gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc
    23821 tctccaatct cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa
    23881 aagaatttga agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt
    23941 tttcagaaca tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt
    24001 taaaaacttg tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat
    24061 gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta
    24121 atgactgcgg gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc
    24181 tgctgcttct taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat
    24241 cgttttttga acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt
    24301 atgataaaga aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc
    24361 aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt
    24421 acaattatgt cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag
    24481 aattcgcctt tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt
    24541 tgattgcgga agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa
    24601 aagaactagt tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt
    24661 tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc
    24721 cagaaaaatt ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa
    24781 ttttttccag ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc
    24841 gggcttttgt gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct
    24901 atatcgtcac aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa
    24961 ctactttcga ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt
    25021 atgcgcagca gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg
    25081 tccaatatga aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag
    25141 ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa
    25201 gatgcccatt tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg
    25261 gacatctacg ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac
    25321 caacgccgct agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc
    25381 agctgaaaca agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc
    25441 attgttctcg ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa
    25501 atccttgatt gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc
    25561 cagtacaagt ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc
    25621 tctcctcttc tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact
    25681 gttcgaatga taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact
    25741 gactcgatga tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc
    25801 agttttcttg tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac
    25861 caatcaaaaa gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa
    25921 atatttaaaa tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac
    25981 gctacctact ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt
    26041 cgtctattta aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc
    26101 ccatttttct ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa
    26161 ataaggctta gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac
    26221 ccaaatttct aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat
    26281 ttttatttac aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc
    26341 gtcctatcat ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca
    26401 aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg
    26461 cccttggcgc tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat
    26521 atgatgaatt cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga
    26581 agacattgga ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc
    26641 caatatggca gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac
    26701 cttttattca ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga
    26761 tgaatattgg aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg
    26821 tcgcatgctt aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc
    26881 tgttactgag ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg
    26941 aacctttgac aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact
    27001 gggattcaat aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga
    27061 agaagaaaaa ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt
    27121 tgaaacaaac ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc
    27181 tcaagaattg cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct
    27241 agtgggcgtc aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga
    27301 gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt
    27361 caaagaaaca ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact
    27421 gactcaagaa gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg
    27481 gtccattaca gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga
    27541 ttatggtgca gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa
    27601 tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac
    27661 gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc
    27721 cttgaattca tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat
    27781 gtctatcgtc gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat
    27841 ttggggttat ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa
    27901 aatgaatgga aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg
    27961 accagtgaat attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc
    28021 tgtctatatg ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa
    28081 agtcattcct aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta
    28141 tcagatgatc tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt
    28201 tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac
    28261 tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct
    28321 gcatcaagta ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga
    28381 cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttgtg aaaaagctgc
    28441 cctgcatcaa gtaataagaa cacccaagct aaaactgctt cttatgtttt tgacttggtt
    28501 ggacttatta ccgcagatgc aagcttttga acaccattta ccaagaggtt atgaatgccc
    28561 tgcatcaagt aataagagca gccaagctaa aacagcttct tatgtttttg acttggttag
    28621 acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaaggcg
    28681 tttagtttcg agtatgaaga aaaattttgt gaaaatggtt cctcacgttt ttgcataaat
    28741 ttgacttaat accgagaaac tgatcccttc tgtcataacc tctttttatt tatgtctgtt
    28801 tctttttgaa aacaactatt ttttcaacgt ttactctctt gccaatccaa catcatctaa
    28861 cggatacaac tatttcaatg attaccatta cactaaaaaa tcggaaacac acctctccga
    28921 acacaaatac ataagactat agaatgtttc atttaagaaa aaaagcgttt cattatcaaa
    28981 aaggtaagat ctcctttaaa atgaaattat ctgtagtact aataatgcag acaattttat
    29041 gaatctgaaa ggagcatctc caatgaaagc agtcacttgg caaggaaaac agaaaatgga
    29101 aatcagaaac gtagatgatc caaaaatatt agaaccaaca gatgcaatca tccgtatcac
    29161 agcaacagcc atttgcggct ccgatctgca tctttaccat catggtgaag cagttttgga
    29221 aaaagggtat gttgtaggac acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt
    29281 tcaaacattg aaaaaagggc aacgtgtggt tatccctttt aatattagtt gtggtcattg
    29341 tcatttctgc ctgaatcaca tggaaagcca atgtgatgaa tcaaatccag atcagttata
    29401 tggcgggctt ttcggatttg gcaagttgaa cggtaatcat tggggtggtc aagcggaata
    29461 tctaagagtt ccttatgcag actctacttc atttgttgta ccagataatg atcttcctga
    29521 tgaaaaagtt ttatttctat cagatatttt gcccacagct tattggagcg ttgaaaacgc
    29581 aggagtaaaa aaaggcgata cagttgtgat tctaggttct ggtccagtcg gtctattcgc
    29641 tcaaaaattc gctgttatgg ctggagcaga gcgcgtaatc gcagtagatc cagtacagca
    29701 ccgtctagat aaagcggcta attacaatgg ggttgaaact tatttcttag aagacacggc
    29761 acaagctgga acagaattgt atgaactggc taaaggtggc gcagatgtca ttatcgattg
    29821 tgtaggaatg gacggattag agcctgtcaa agaaaaagcg aaaaatttag taagcttaca
    29881 aagcggcaca atctctccat tacagatggc atcgcaagct gtcaaaaaat ttggaacagt
    29941 ccaagtaact ggtgtttata tgactccagc ttcttcttac ccgctgcaag aattttttat
    30001 gcgggatatc gctgtcaagc atggacaggc accaattatc catctaatgc caaaaattta
    30061 cgcaatgatt gcagaaggac tattcgatcc tacacaaatc attactcatt cgctgccact
    30121 atcagaagca gcacaagcat atgagatctt tgacaaaaaa gaagataaaa atataaaagt
    30181 tgttttgaaa ccttgaattc agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa
    30241 acgaaatacc catgagatcg aaataggaaa agaagaagat atcgaaatac gaggacgtcg
    30301 cttccgcttc tatttccaaa atcgttacac attgatctca ctggctgtcg atttattaac
    30361 cgggattttc tatatttttg gaagccttgc ttccttaact aaaatccccg atcgatatgg
    30421 cacctatttt tatttggctg gtgctatttt cttgacgatt cgtcctattc tcagaatcgt
    30481 aaggaacatt tttatttacg acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga
    30541 ggcatgagaa tttttcaaat ctcatgcctc ttttttcacc ctatcgtatc actttgatac
    30601 agacgagcgg tcgtcccact ttcttccttg accattgtca aaaatgtcca ctcgtatcgt
    30661 tcataaaacg tggtatgatc tgtaattaag taaagttttt cgtacccaag ttgatgcatt
    30721 tcttttctga tttccgctaa tatccgcttg gctatcccct gcttacgctt ttccggttca
    30781 acaaacaggg cacaaagatt tggtgtcaga tcagggcggt tatggaagtc gttttcaatg
    30841 acgcccgctc ctccgacgat ggtttctttt tcatctaaga ccacatacca ttgaggaata
    30901 ctttccttat tagcgattcc agtacgaata ctttcctcat acacaacagc aggaactccc
    30961 cattttgatg aaaaccaatt cgcgctttgt tttactaatt ctgggtgttc tcttaatttg
    31021 atccagttat atgtaaccaa tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt
    31081 atacaagtct ttatgaaaat tagcaatgac aacaatagtg atgtaaaaaa aaaacggaaa
    31141 acaatcactt gttctccgtt ttttgattga tcaagcatct aaagcttctt ttcttctttc
    31201 ttggatcgta gctaccttga cgaacggtag atagatcaaa atagaaagaa ccaaattgac
    31261 agcggctagt acaccacctg caatactttt tgttgccaat acaccgccaa taattggtgg
    31321 agttacccat ggaggcataa cggtagctgc tggcactaaa cctgtagaag tcgcaaagta
    31381 agcaacacta actaagacca ttggtgtcaa aataaatgga atgaacatga tcgggttcaa
    31441 cacgataggt aacccaaaca tcgttggttc attaatattg aagacacctg gcgcaatcga
    31501 caagtttgtt acaaccatat atggtttgtt tttacgtcct actaaataaa tagctaacag
    31561 taaacctaaa gttgcacctg ttcctcctaa gttgacaaag ctgtcaaaga aaggtttgtt
    31621 cacgataaac ggtgcgctat gcccagctgc gattgcatta acgtttgctt caatggctgg
    31681 cgcattgatt gtttgcatca aaggatcgac catattcgct ccgtgtaaac caaagaacca
    31741 taagaatggt gtgataaatg caagcagtaa ggcagatgga taagagtttg caagtcccat
    31801 aaatggttct tggaccactg tatagaaaga acctacgata tcatcaacac cgaatgcagc
    31861 aaaaattgca gcaactaaag caaacgcaga gatagtgatc attgctggta gtaatgaagc
    31921 aaatgctttt gcaactgccg gtggcacacc atcaggcatt ttgatgatca atttttcatt
    31981 tccgcttaaa cgtacaaaga tttctgttga aataatccca acaataatag caataaacaa
    32041 accgtttgag ctcataccta atgctccgcc taaagcgaag aaagagccta aagaaatcac
    32101 acctgcggaa acaccgtctt ttcggtagcc gttagctaaa tgataagcaa gcaagaaagc
    32161 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    32221 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    32281 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    32341 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    32401 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    32461 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    32521 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    32581 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    32641 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    32701 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    32761 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    32821 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    32881 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    32941 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    33001 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    33061 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    33121 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    33181 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    33241 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    33301 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    33361 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    33421 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    33481 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    33541 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    33601 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    33661 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    33721 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    33781 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    33841 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    33901 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    33961 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    34021 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    34081 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    34141 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    34201 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    34261 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    34321 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    34381 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    34441 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    34501 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    34561 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    34621 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    34681 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    34741 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    34801 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    34861 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    34921 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    34981 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    35041 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    35101 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    35161 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    35221 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    35281 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    35341 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    35401 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    35461 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    35521 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    35581 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    35641 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    35701 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    35761 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    35821 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    35881 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    35941 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    36001 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    36061 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    36121 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    36181 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    36241 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    36301 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    36361 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    36421 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    36481 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    36541 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    36601 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    36661 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    36721 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    36781 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    36841 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    36901 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    36961 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    37021 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    37081 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    37141 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    37201 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    37261 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    37321 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    37381 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    37441 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    37501 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    37561 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    37621 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    37681 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    37741 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    37801 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    37861 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    37921 agtcattttt ttgtatcttc tccattatag ttggatatac caatatataa tgcttttcct
    37981 tgtcttacaa gttgcatcaa agcttctgct gtttcttcca aaggtgtatc agggtctggg
    38041 cgatgatgat agaagatgtc aacataatct agccccattc tttggagact ttggtcacaa
    38101 cttgaaatga tggatttacg agagccccat tccccatatg gcccttccca catatgataa
    38161 cctgctttgc tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata
    38221 cgcccaaagt ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa
    38281 tgggtaattc ccatgtcgaa tgcgcctctt aaaatctctc tttgattatg gataggatca
    38341 taatcaccaa aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga
    38401 ccggatttac gatattccat tttttcataa cgtgcactat ctgcaatata caatttttct
    38461 cctcctcatc aaatcttttt aaaacgcttt cttttttcta tttttagttt actacatttt
    38521 aaagaaaagg agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac
    38581 tcgttgatag atcagaaatt gattacgtgc ttgctggatt tttttataac gtttcggttg
    38641 ttttttgtaa aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat
    38701 ttttgtgacg attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc
    38761 cgctaattct ttttgtcgtt cgccttgata aaaaatcact ggacgatatt gcttccctct
    38821 atcttgaaat tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa
    38881 ctcgctataa cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt
    38941 gtaaccacca cttacttgat cataatttgg tttttcaaca tgtccgcccg tgtatcctga
    39001 caaaacagat aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc
    39061 ccctgcaaat actgcacgtt cttgatagtc agcatcttgc atggcatgct tggaaaagtc
    39121 atatttttgc tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg
    39181 cgtcagattc attcggatag ctaatggacg tagtgttgct tctagttttg ataactgttc
    39241 tttaacggat atattttctc ttatgtcatt ctttgtctga atcaatactt ttcgttccca
    39301 atctcgtgta ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc
    39361 atccatagat tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc
    39421 ataaagtgag gaaataagcg attacttcat ccccctacag atttttcgta taaaaaagag
    39481 agaatcctta tcgacagatt ctccttttta atcaatttat tttataaatg aaaagttaat
    39541 ttacctttta tggaaaacta ttttaaaaaa taaacataaa tatgcagtta atgtaacaag
    39601 ataagaaata atagtcgtta ctgtataact tgtgcttact ttaatatcag caactccttt
    39661 gactccgctt atttgtacca atccattagc attcacctgc tcctttacag gttgatgatt
    39721 tatttctaca ttcgttccta aataagcata tacaggaagt gtgattactt ggctcttctc
    39781 attagttact ctatatatga tagaactacc agttgtttta taaggaaggt ttacccattg
    39841 actgccaatc tttgcttcgt gttttattat atgctcctta ttctccaatg aagcgatagg
    39901 agaataatca tataaaaaat aatccattcc tttagtggta tacgcctcta ctgtttcttt
    39961 gtttacaaca atatgtcccg gctgagaaat tattttttga ttgaaatttt ttttactaga
    40021 aacagttaaa gtgattaata acaaaacagt cataaataaa taaaatagtt ctcttgtctt
    40081 attgtatctt ctatttttga aaaatatcat cgttaatatc agagaagaga atattatttg
    40141 caagcttaag atccgccaag gaaattgtaa caatttggct ggcgtatttt gcagaagata
    40201 ccatggcacc aaactagttg tagctagcca agtcaaacac gtaccataat aaatcttttt
    40261 ttctatgttt gttaattttt taaataaaat caggggaata cataaagata acagaccaac
    40321 tagtcccatt gagtaagtag ttaagtcgtt gttcatagct tcagtcagac tatcgaacac
    40381 attagatgca tgagcatata aatttgtcac gctaggtttg ttgatttttt ggtagagcat
    40441 ttgttcaaac atcggatacc aaaaagcact cgtcagaacc aatgtcattc ctgccgcact
    40501 aaataatttt ttaaatatag caataaaatc tgcattcttg aaacctttag aaaccaaacg
    40561 aataaaaatg ataattaatg ctgtactcat taatgttgat agtacatggc tgtacactag
    40621 caaactcata ctcaatgcta gctttacaga cggatacttt tcttcttttc cagagatgat
    40681 ttgataagca taaaataata cagggataac aagtgttatt gcgatagctt cggcaattgc
    40741 ggaacgatag tagatgtcta ctgtccgata agcagagaaa atatagagac aactaaataa
    40801 aaatgccgct tttttttgtt ttaaaaaccg ctcgccataa tgataagcta cgagtgttgt
    40861 acacaagttc aataaccaaa cgtaaataat atagcttaca atcaaatttt ttgtcatcca
    40921 atataacatt acagctggaa aaaaagtcag aaagggataa aaataattta caccatatcc
    40981 agtataattg aatgtctcat aattaatcgg actggtaaaa atagtcgata accccttgat
    41041 tcgagataag tgaaaagaaa gatctccagt ggtataaata ctctgtgtat ttttgaaaaa
    41101 gatgaaagaa tatatactac taactataat taatccactc aatattacta cttttttatt
    41161 tattttcatt cttcttgttt atctcctttt tagtcacata tcgagaatct ttcaacttat
    41221 taggtgcaaa tcgtttaaaa cttgaataca ccactaaaca ataagtcaga atactgatcc
    41281 ataaagccat gagagcagtt ggagtatatt ttgttgtaat ttctatctta ttttctccag
    41341 catttacatc cacattcact aagccatgtt ttttataaga gtcatttacg actttaccgt
    41401 ttacttttac ctgtgtacct aaataagcat atactggtac tgtcactttt tgctttttgt
    41461 tgcttgccac tgtgtaagta atgttattat ctgcagcttt atacttccct ttttcccacg
    41521 attctcctac ttttacttca tgtttttcta agtgaggcct ttcttttatc gcttctgttg
    41581 gggcgtaatc gtataatcct cccatatctg atgttgtata acgtgggagt gtttcttcat
    41641 tgataacaat atgaccgtgt tttgattgga taacttttgc gtaattttct tttgctgcta
    41701 tagacgtgac gagtagaaac accacagtag cgccaatgat taaccatgaa tattttttat
    41761 tcatagtctt tttcataaaa acaagcgtta tgatcattga agaaaaaaag atttggatac
    41821 ctaagatacg ccaaggaaac tggagcaagc ttatcggtgt tttttgaaac aaaaaccatg
    41881 gaaataaatt agttacgcat acccatgtag aaactacgcc aacatagatg attttttctt
    41941 ttttctccat ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat cctaataacc
    42001 ccatagtgta tgttgtaata tcgttattta acgcttgaat gatactatct gaaatagaaa
    42061 gtgcttctag ctgcaaatct gttttgaagg gactgtgtat ttcttgataa ttcatctgct
    42121 gcaacatagg ataccaaaaa tagctagtta atgcacaagt caaaaaaaca gcctttacta
    42181 aattcacgac cacaaatttt atatcgtttt ttatttgctt gcttaaaaac aaacgaacta
    42241 taatcataca agcaatcaat gcactacaca atagtgtagt taagacatga gtatacacaa
    42301 gtagacttat actgacagat aaagcgatga cttttttttc tctattgaat atcaatccat
    42361 aagcataata taaaacagct gggataatcg taagagcgat tgcttctgca attgcggatc
    42421 gataatagat atcaactgtt cgataagttg caaaagtata taaacaacta aataaaaaag
    42481 cagcatctat ctttttaaaa aacttttctc cataataaaa a
//
LOCUS       DADXTH010000015        42403 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.015, whole genome shotgun sequence.
ACCESSION   DADXTH010000015 DADXTH010000000
VERSION     DADXTH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.015"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(148..2301)
                     /locus_tag="IWI97_000773"
     CDS             complement(148..2301)
                     /locus_tag="IWI97_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBC2462296.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="IWI97_000774"
     CDS             complement(2595..3443)
                     /locus_tag="IWI97_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBC2462297.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWI97_000775"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWI97_000775"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBC2462298.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="IWI97_000776"
     CDS             complement(5944..6123)
                     /locus_tag="IWI97_000776"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBC2462299.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWI97_000777"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWI97_000777"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBC2462300.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="IWI97_000778"
     CDS             8271..10550
                     /locus_tag="IWI97_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2462301.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="IWI97_000779"
     CDS             complement(10643..11290)
                     /locus_tag="IWI97_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462302.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="IWI97_000780"
     CDS             complement(11469..12188)
                     /locus_tag="IWI97_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2462303.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="IWI97_000781"
     CDS             complement(12319..13470)
                     /locus_tag="IWI97_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBC2462304.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="IWI97_000782"
     CDS             complement(13687..14427)
                     /locus_tag="IWI97_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2462305.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWI97_000783"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWI97_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBC2462306.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="IWI97_000784"
     CDS             complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="IWI97_000784"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBC2462307.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16038)
                     /locus_tag="IWI97_000785"
     CDS             complement(15388..16038)
                     /locus_tag="IWI97_000785"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBC2462308.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWI97_000786"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWI97_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBC2462309.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWI97_000787"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWI97_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBC2462310.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="IWI97_000788"
     CDS             complement(17481..18008)
                     /locus_tag="IWI97_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBC2462311.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="IWI97_000789"
     CDS             complement(18188..18973)
                     /locus_tag="IWI97_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBC2462312.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /locus_tag="IWI97_000790"
     CDS             complement(18966..19835)
                     /locus_tag="IWI97_000790"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBC2462313.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="IWI97_000791"
     CDS             complement(19836..21215)
                     /locus_tag="IWI97_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBC2462314.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWI97_000792"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWI97_000792"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBC2462315.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWI97_000793"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWI97_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBC2462316.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWI97_000794"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWI97_000794"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBC2462317.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGTKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWI97_000795"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWI97_000795"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBC2462318.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWI97_000796"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWI97_000796"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBC2462319.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="IWI97_000797"
     CDS             complement(25173..25397)
                     /locus_tag="IWI97_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBC2462320.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="IWI97_000798"
     CDS             complement(25450..26412)
                     /locus_tag="IWI97_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBC2462321.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="IWI97_000799"
     CDS             complement(26422..26862)
                     /locus_tag="IWI97_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2462322.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="IWI97_000800"
     CDS             27168..28133
                     /locus_tag="IWI97_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBC2462323.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="IWI97_000801"
     CDS             complement(28190..28420)
                     /locus_tag="IWI97_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBC2462324.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="IWI97_000802"
     CDS             complement(28506..29393)
                     /locus_tag="IWI97_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBC2462325.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="IWI97_000803"
     CDS             complement(29482..30705)
                     /locus_tag="IWI97_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBC2462326.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31768)
                     /locus_tag="IWI97_000804"
     CDS             complement(30722..31768)
                     /locus_tag="IWI97_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBC2462327.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWI97_000805"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWI97_000805"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBC2462328.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="IWI97_000806"
     CDS             complement(33659..33925)
                     /locus_tag="IWI97_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBC2462329.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="IWI97_000807"
     CDS             34404..36638
                     /locus_tag="IWI97_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2462330.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="IWI97_000808"
     CDS             36786..37106
                     /locus_tag="IWI97_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBC2462331.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37311..37535)
                     /locus_tag="IWI97_000809"
     CDS             complement(37311..37535)
                     /locus_tag="IWI97_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBC2462332.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37733..39313)
                     /locus_tag="IWI97_000810"
     CDS             complement(37733..39313)
                     /locus_tag="IWI97_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBC2462333.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39714..41090)
                     /locus_tag="IWI97_000811"
     CDS             complement(39714..41090)
                     /locus_tag="IWI97_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBC2462334.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41218..42336)
                     /locus_tag="IWI97_000812"
     CDS             complement(41218..42336)
                     /locus_tag="IWI97_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2462335.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT        11987 a         9125 c         7325 g        13966 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcagatatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgta cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggtagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattcctttt cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcatatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc gaaagcagaa
    37201 gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    37261 ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt ctagcttttt
    37321 gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg cgtgtcttta
    37381 tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat ttgctcgttt
    37441 ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc ttttttctgt
    37501 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    37561 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    37621 gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa caaaatatga gaggctgttt
    37681 ttgtttggct attcttatta cttgatgcag ggcagcttgt gtcaagactt ctttacatca
    37741 aactttttaa ttttacattg ggatatttat ccgcaaacca gcgttctgca aattggttct
    37801 cgaataagaa caacggttgg tcgaaacggt cacgagccaa gatattgcga cttgaactca
    37861 tcttctcatc caaatcttct ggatcgatcc agcgagcgat tttgtgtccc attggagaca
    37921 tgattacttc ggcgttatac tcatttaaca tacgatattg gaatacttcg aattgtagct
    37981 gaccaacagc tccaatgata tattcttccg ttagatatgt tttataaagc tgaatcgctc
    38041 cttcttgaac taattgatag attcctttgt ggaaggattt ttgtttcatg acattttttg
    38101 cagtcacttt cataaacagc tctggagtaa atgaagggag ttcttcataa gcaactttta
    38161 gttttccttc gaacaatgta tccccaatct gataattgcc tgtatcatat acaccgatga
    38221 tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc tgccataaat tgtgtgacat
    38281 tgcttaattt cagtttttta tttgtacgtt ccaaccagac atccattcct cgttcgaatg
    38341 ttccagaaca gattcggaca aaagcgattc ggtcacgatg ggctggattc atatttgctt
    38401 ggatcttgaa tacgaaacct gaaaattctt cctcataagg acttaccatc tgatcatctt
    38461 gtgttttgtg tccataagga gctggtgcca attcaacgaa agtctctaaa aaagtctgta
    38521 caccgaagtt agtcaatgca gaaccaaaaa atactggtgt ctgatcgcca cgagcaattt
    38581 tttcttgatt aaatgcatct ccagcctctt tgattagttc tacttcttct aagacttgtt
    38641 catataagga attttcagtt agaggatgat tttcgggaag cagtccatct tcatccaatt
    38701 ctactaatcg ttcatcctga taattttccg gacgataaaa ctctacacga ttatggtaga
    38761 tatcataaag accttctagc cctttaccca taccgattgg ccaattcatc ggataagatt
    38821 cgatatctaa tagctcttct aattcttcta acaattccaa tggttcacga ccatcacgat
    38881 ctagtttgtt gataaaagta aatatcggga tgccacgttt ttttacaact tggaataatt
    38941 tctttgtctg tgcttcgatc ccttttgcac tatcgattac catgaccgca ctatccacgg
    39001 ccatcaaagt acgataagta tcttcagaaa aatcttcatg tcctggtgta tccaagatat
    39061 ttactcgttt cccttgataa tcaaactgca taacagaact agtaacagaa atcccacgtt
    39121 gtttctcgat ttccatccag tctgatttgg caaaattacc tgtttttttg ccttttacgg
    39181 tcccggcttg acggatagcc ccaccaaaca atagtaattg ttctgtaatc gttgttttcc
    39241 cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt gtccacttgt tgctttaatt
    39301 ttggattatt cataacctaa ctccatttct tccattcaat tatttatatt ttctctaggc
    39361 acagattgtc cgatctctta aagaaagaca atttcatata gggctgtgac aaaagccgtg
    39421 tagcagtctc tttttccgag taaacggcgt tctagagaca gcctatcagc aataagccca
    39481 cccaagccaa aaatacgaaa agctgttttt gcttggctgc tcttattact taatgcagga
    39541 tagctttttc acaacctttg tcaactacta caactgaatc agtatagcat ggattcagcg
    39601 aatgaaaaag ccaccaaacc ataagagtca aatatttttt tgtcagctga ctaaaagaaa
    39661 agacgtgttg cttgtttatt caagcaacac gtctttcata accaagtttc caattatctt
    39721 cttggctcgt catcttcgaa ttcttttctg aagtatcttc gttcttcttc aggttccgct
    39781 tctgtttctt ccggatcatg aaaaatgaca cggattttaa ggattcgaga accttccatt
    39841 tcttcagaaa ccagcgtaat attatcgaca tcaaacgata acttttcgcc ttcatccgga
    39901 atcatgccta atgctgtaat caaataacca gccatcgtat ctacatcact catatgcaga
    39961 tcactgtcaa aagcttcatt gaactcatca atcagcatac gtccttggat gatatattcg
    40021 tgttcatcga ttttttcata aagattctca acttcatctg attcatcatc gatttctccg
    40081 acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa cgacgccgcc atattcatct
    40141 aataaaatag ccatctgatt ctgcgtcttt ttcatttcat ataacagatc atcaataaag
    40201 accgtttcag gaacgaataa aggctcctgc atgattttct tgatatctaa attatcaaag
    40261 ccgaatttat gggcagcttt tagtaaattc tttgtatgaa ggattccgac tactttatct
    40321 ttgtcttcgt tataaacagg gattcttgaa tagttttcgg ataaaacttc attcacattt
    40381 tcttccacag tgtcgttgat atcaatcata aatgcatccg ttcgtgggac catgacttct
    40441 cgggcaactt tcgtatcaag agaaaaaacc ccttggagca tttctaattc ttcattattc
    40501 aatacgcctt cgttttcaag catataacgc atttcatctc tggtcatttt tgaatcttca
    40561 tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg tggaagcaga aagcagccag
    40621 acgaagggct tagctactac accaatcaag cgtatcagcc ctgaagtgaa ctttgcgact
    40681 tcttctgatt tatttaaagc aatacgttta gggtacaatt ctccaaaaac aatcgataca
    40741 taggtcaaaa tagccagcac aatgatattt gcaatatttt ttgccgctgt gccgccacct
    40801 aataccggag ctaaccgttc cgataaagta ttagctagtg aagcaccaga caaaatgttg
    40861 accaaagtga ttcctacttg gattgtcgat aggaaattat ttggattctg caatattttt
    40921 aaaagctttt gagatttcac atctccttca tcagcttttt gctcgattcg attcttattg
    40981 atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat tgatcaatgt caaaacgatc
    41041 aataataaaa tctgcgctaa cagcgactga ctctcagggt cagcattcat aatcgcattc
    41101 tctccttata aaattaattt ttagtttacg atagaaaaaa acgtggcact atagccacgt
    41161 taaaatggta tcacaaagca attttctttg caataaaaac aagtgtacta aaactaccta
    41221 cgaggtcgta gcaattttct tcttgttttc attttgcttg tcttctttga cgattttgat
    41281 tatataggaa atgatcgtct tgcagattgc gtaaaaagga acacctaaga agataccaag
    41341 caatccggca atatttccag ctactagaag aacgataatg atcgttaatg gatggatttt
    41401 cagtgatttc ccaattacat taggataaat gatattccca tccaactgtt ggacgattag
    41461 cacaaccaca caacaaagta atgcttttac cggatcattg aagactgtta ctagaaatgc
    41521 aggtgctagt cctagataag gtcctaaata tggaattagg tttgtcaatc ccgcaatgac
    41581 cccaaataag aaagcataat ccacaccaat taccatatat cctaaaaatg tgaaggtacc
    41641 aacgaataaa cattcgattg cttgtccgct aatgtaatta gccaatgttt tattcagttg
    41701 ccccaataac tccacaattt ggtttcttct tttttctggg aaaaactgtt ggatgtttgg
    41761 cactagtttt tctccgtctt tcaacatata gaacaagatg aatggtgctg taatgattac
    41821 tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa ctattggata ctccactaag
    41881 gaattgctga atgattgtcc cgtaagaaat gtctaattgc tcaaaatatt tattgatatc
    41941 tacttccttg aacaaaggca attcggctac ctcttttaac cagctttcta cctgtttgat
    42001 gaaatcaggc atattagatg ccaatgagga aatttgctgt actagacttg ggatgacact
    42061 aagtaaaatc caaataattg ctgcaactaa aaggagcagc acaattgcga cagcccatat
    42121 tcttttgatt tttgctttca tcaacagctt gacaatagga ttcaataaat agtaaagaaa
    42181 gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat gtcccgatcg gttcgaaaat
    42241 aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg atcaataatt caagcgacca
    42301 aaacattagt ttagactgtc taagtttttc aaacaataga tttcctcctt tctttcttac
    42361 tagtatatat tatattcgtt aaaaattctt ttattctttt ttt
//
LOCUS       DADXTH010000016        41978 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.016, whole genome shotgun sequence.
ACCESSION   DADXTH010000016 DADXTH010000000
VERSION     DADXTH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.016"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            281..1321
                     /locus_tag="IWI97_000813"
     CDS             281..1321
                     /locus_tag="IWI97_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBC2462336.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2145..2477
                     /locus_tag="IWI97_000814"
     CDS             2145..2477
                     /locus_tag="IWI97_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBC2462337.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3096..3437
                     /locus_tag="IWI97_000815"
     CDS             3096..3437
                     /locus_tag="IWI97_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBC2462338.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3456..3746
                     /locus_tag="IWI97_000816"
     CDS             3456..3746
                     /locus_tag="IWI97_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462339.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIINEQKSCVLSIDSTLV"
     gene            3938..4171
                     /locus_tag="IWI97_000817"
     CDS             3938..4171
                     /locus_tag="IWI97_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462340.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            4335..4505
                     /locus_tag="IWI97_000818"
     CDS             4335..4505
                     /locus_tag="IWI97_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462341.1"
                     /translation="MKQKHLKRLSVAQYINDMQLTGKEKACLLGSMKNFEQLRRTYVK
                     KSSNCQLLLEVS"
     gene            4591..5262
                     /locus_tag="IWI97_000819"
     CDS             4591..5262
                     /locus_tag="IWI97_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBC2462342.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5315..7291
                     /locus_tag="IWI97_000820"
     CDS             5315..7291
                     /locus_tag="IWI97_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBC2462343.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITRLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKVSV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            7306..8058
                     /locus_tag="IWI97_000821"
     CDS             7306..8058
                     /locus_tag="IWI97_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2462344.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            8074..8835
                     /locus_tag="IWI97_000822"
     CDS             8074..8835
                     /locus_tag="IWI97_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462345.1"
                     /translation="MRNRKWLFVCAAALFTFTAYTQLTVADSQESTDSTEQTIQTSDS
                     SAIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKKAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            8848..9108
                     /locus_tag="IWI97_000823"
     CDS             8848..9108
                     /locus_tag="IWI97_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462346.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            9105..11195
                     /locus_tag="IWI97_000824"
     CDS             9105..11195
                     /locus_tag="IWI97_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBC2462347.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNKTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDNMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKELNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            11222..11686
                     /locus_tag="IWI97_000825"
     CDS             11222..11686
                     /locus_tag="IWI97_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462348.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            11683..11925
                     /locus_tag="IWI97_000826"
     CDS             11683..11925
                     /locus_tag="IWI97_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBC2462349.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            11943..12245
                     /locus_tag="IWI97_000827"
     CDS             11943..12245
                     /locus_tag="IWI97_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462350.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            12315..14378
                     /locus_tag="IWI97_000828"
     CDS             12315..14378
                     /locus_tag="IWI97_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462351.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQLELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            14378..16987
                     /locus_tag="IWI97_000829"
     CDS             14378..16987
                     /locus_tag="IWI97_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBC2462352.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGEK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRSMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            16984..19362
                     /locus_tag="IWI97_000830"
     CDS             16984..19362
                     /locus_tag="IWI97_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462353.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVTLDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQIEKASEVKEGLKGAYGEGQV
                     NAAVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNI
                     NSENEKHISDLPKRNQEVQTDEDSIDLPNDNGVEVGTEKSTKIGLPKAQNLPMKDSRL
                     NQFKENDQSFVHQSTAGTVQMKSSRLPENGNLTTISYNLSESGETENASVQNIVTREA
                     APKPIQNVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP"
     gene            19414..19746
                     /locus_tag="IWI97_000831"
     CDS             19414..19746
                     /locus_tag="IWI97_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462354.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19747..20340
                     /locus_tag="IWI97_000832"
     CDS             19747..20340
                     /locus_tag="IWI97_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462355.1"
                     /translation="MRVKGQNSVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFIIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            20503..22527
                     /locus_tag="IWI97_000833"
     CDS             20503..22527
                     /locus_tag="IWI97_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBC2462356.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            22527..23744
                     /locus_tag="IWI97_000834"
     CDS             22527..23744
                     /locus_tag="IWI97_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBC2462357.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVYVTLF
                     LILAAGSIDNSDSDSSSGGEAFGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAA
                     VKYKLLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGW
                     YMKFPNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYT
                     GCMSIITSNKLTEYDEFAIKHWGEGNNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGT
                     NPGGEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGL
                     NMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGT
                     WIDPLPFLNSSKK"
     gene            23760..24404
                     /locus_tag="IWI97_000835"
     CDS             23760..24404
                     /locus_tag="IWI97_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462358.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSDAQVKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            24415..25221
                     /locus_tag="IWI97_000836"
     CDS             24415..25221
                     /locus_tag="IWI97_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462359.1"
                     /translation="MSKKKGARPRFKKRKSYLIWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            25244..25474
                     /locus_tag="IWI97_000837"
     CDS             25244..25474
                     /locus_tag="IWI97_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462360.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYIAAKKYFTADNK"
     gene            25987..26283
                     /locus_tag="IWI97_000838"
     CDS             25987..26283
                     /locus_tag="IWI97_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462361.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLIEND
                     FDRPLMEYKKEKFDQLLEKFSEIQSYNTKILEEYVSQNNRILEILIEQNRGSEL"
     gene            26285..26707
                     /locus_tag="IWI97_000839"
     CDS             26285..26707
                     /locus_tag="IWI97_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462362.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEMEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVYARVDPFDPTKILKR"
     gene            26726..28318
                     /locus_tag="IWI97_000840"
     CDS             26726..28318
                     /locus_tag="IWI97_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462363.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYTLEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIEGVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            28330..28665
                     /locus_tag="IWI97_000841"
     CDS             28330..28665
                     /locus_tag="IWI97_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462364.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            28811..29005
                     /locus_tag="IWI97_000842"
     CDS             28811..29005
                     /locus_tag="IWI97_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462365.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYRSGDI
                     LALNLITKTFVDAVENYPFV"
     gene            29018..31390
                     /locus_tag="IWI97_000843"
     CDS             29018..31390
                     /locus_tag="IWI97_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462366.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLARLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNGPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            31451..32920
                     /locus_tag="IWI97_000844"
     CDS             31451..32920
                     /locus_tag="IWI97_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBC2462367.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGLAVVNKGNDYRLEDHLSFV
                     FERKKQYFSWNSRNIHGDIIDLADLFFVDPSITDKKERFKAATNFILKNENKLERVEN
                     LHFETEKYKDHPIDYQPLTEKGRSYLKEERKLPDWLINYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFFWKDSQTKETVGASYQGTIVDFDRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELSR
                     KQTFPQSIEVCVDNDRAGHIFYEKEQLKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMNVEPEAIMAIHKTETNLQLTNQLVSAYDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGIQHERE"
     gene            32931..35135
                     /gene="topB"
                     /locus_tag="IWI97_000845"
     CDS             32931..35135
                     /gene="topB"
                     /locus_tag="IWI97_000845"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBC2462368.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKTSRKDSYFEIQDRLFSGKTVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYRYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWSIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTTQNGTFGAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEALQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVIKGETVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQTTNFGSTLQEVKA
                     ASEKQEAARHLGTCPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QKGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            35151..35300
                     /locus_tag="IWI97_000846"
     CDS             35151..35300
                     /locus_tag="IWI97_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462369.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRAKKRRLQQLKNQQRIQKE
                     IELDR"
     gene            35380..35976
                     /locus_tag="IWI97_000847"
     CDS             35380..35976
                     /locus_tag="IWI97_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBC2462370.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            35989..36888
                     /locus_tag="IWI97_000848"
     CDS             35989..36888
                     /locus_tag="IWI97_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBC2462371.1"
                     /translation="MTPTQLKAKVKNISSEKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKTYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYKETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            36891..37376
                     /gene="ssb"
                     /locus_tag="IWI97_000849"
     CDS             36891..37376
                     /gene="ssb"
                     /locus_tag="IWI97_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBC2462372.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVTDNFQL
                     LESKAATESRAHADQSSTSPSSTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            37398..37655
                     /locus_tag="IWI97_000850"
     CDS             37398..37655
                     /locus_tag="IWI97_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462373.1"
                     /translation="MAKNISLTMDLLIPVIAFIDGISLGITEDYLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(37814..37936)
                     /locus_tag="IWI97_000851"
     CDS             complement(37814..37936)
                     /locus_tag="IWI97_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBC2462374.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            38090..38350
                     /locus_tag="IWI97_000852"
     CDS             38090..38350
                     /locus_tag="IWI97_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462375.1"
                     /translation="MEHYTGLLDKIRVIKTQPLLVRFTLRTNHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVTESMQIRNPDHFTRLMGI"
     gene            38799..39005
                     /locus_tag="IWI97_000853"
     CDS             38799..39005
                     /locus_tag="IWI97_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBC2462376.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            39005..39256
                     /locus_tag="IWI97_000854"
     CDS             39005..39256
                     /locus_tag="IWI97_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462377.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            39272..39709
                     /locus_tag="IWI97_000855"
     CDS             39272..39709
                     /locus_tag="IWI97_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462378.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AMEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            39702..40340
                     /locus_tag="IWI97_000856"
     CDS             39702..40340
                     /locus_tag="IWI97_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462379.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDT"
     gene            40790..40987
                     /locus_tag="IWI97_000857"
                     /pseudo
     CDS             40790..40987
                     /locus_tag="IWI97_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41028..41708
                     /locus_tag="IWI97_000858"
     CDS             41028..41708
                     /locus_tag="IWI97_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462380.1"
                     /translation="MLKTKKNSYKLKLSILSIICLVDMIISGHLYRVAYTQFIEYKVH
                     YLGLAGNRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENMKLESDLNKLKKIKRRKHKLTKIILLRMMEYKDKATNITIITSLVGYGLLAISI
                     LSIDYGVTILQVLVLAIPMYLSLFIMHEIYKLFVLSKNVEIRVNILVNSYQKELKERR
                     RWRYLLKG"
BASE COUNT        15641 a         6597 c         8548 g        11192 t
ORIGIN      
        1 attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact aaattttatt
       61 catacgtttt tctggtagta tttaattata aataatattt atttaaagta gttcttaatg
      121 actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt taattaaaaa
      181 acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa
      241 ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt
      301 taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc
      361 tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag
      421 attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta
      481 tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa
      541 aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt
      601 agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat
      661 ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat
      721 gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg
      781 tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga
      841 acttaataat aatatattag acactaatag acacaatata gacactgaaa aagaccgcct
      901 acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac
      961 gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc
     1021 tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtggtat
     1081 ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac
     1141 gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct
     1201 gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag
     1261 tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact ggttagagtg
     1321 aaactttact gacaaagtga ttgttgcttc atgaagtaac attaaaatta aaaatttttt
     1381 attattctaa ttatattacg aatattacaa aataatagct attttaatag aatttataag
     1441 ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg ttaaaagaaa
     1501 taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt ttttgagtta
     1561 taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac
     1621 ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg
     1681 ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt tggacaattg
     1741 aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt
     1801 catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc
     1861 cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa
     1921 ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta taaaaatgtc
     1981 cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttgaatgcg
     2041 ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag
     2101 aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa
     2161 ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca
     2221 cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc
     2281 agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt
     2341 taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa
     2401 acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg
     2461 ggcgtttaat cagctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc
     2521 ttgaaaatat tgtcgagact tctaaaagaa cttagaatca ttagaaaaat aaggagtaga
     2581 cttgatctat caacaatttc cttcggggag tgacggggta gatttttacg attggtaacg
     2641 taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt
     2701 aaataatata cacagagtaa acaagaggga atttagaggt tagacaagtt agaatagcgc
     2761 ctagattaat catgagcagc tactaaggga tctaggcttt gacgcaaact catggaataa
     2821 ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac
     2881 atgtagatac ttgcgaggac gttggtcatt ggatcaatcg agcatttacc gctgtctact
     2941 agggtttctc gtttcggacg ccataaacga gtcgatattt tttatgtatt tttaagtaaa
     3001 cgaaaaacga taaaatatga acgagtcctg aataaagtga aaaaattgat ataaaacata
     3061 gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac
     3121 gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa ggttttgtga
     3181 cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattaaac gaggatgatt
     3241 tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg
     3301 aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg
     3361 aagatacttg gtcatatttg agtgattata ttgtcagcgt tgaaatcatt gaatttgtag
     3421 cggataaaga agaataaaaa ctggaggaat aaattatgaa atttttcgaa gtaaaatcac
     3481 catactatta tgcgttaatc gcagcaaaga atgaagaaga atgtgcagaa ttgtatgaac
     3541 aagtagtaac agatatcgaa aatcgtgaag aatttattcg atgtttgcat gagttaaatc
     3601 gaaatgaagc aattcaagag gatgcgaaaa aaattagtga agaaacgtta caaccaatcg
     3661 gtatggagga agctacaaaa gacattttta caattattaa tgaacaaaaa tcttgtgtat
     3721 taagcattga ttctacattg gtttaataga tccaagattc tataattttt ttcaaagtaa
     3781 aaagaaatta ggtaatgatt ctcagaccaa ttacttagga aaataaccat acaaattaga
     3841 ttttacagat aaaggattag aaggaatcac ggcgattaat aaaaaggtag tattacctta
     3901 ggcattgagt aaggaattta tttaggagga aaattagatg gcgcgaccag ttttagatgc
     3961 gatggatatg gatactttga caaaaggaag gtatgtacag ctgttagctc gtggttattc
     4021 acctaaagat gtattcaaag ccctatctaa aggaacagat atggaaaaag aaagattacg
     4081 aaaagaattc gactattgga gagagcataa cggaataaaa gatttaaaac cagcacgtcc
     4141 agttattaga cgaaaaaaat tgaagaagta agaccaaagc gtatggcgta cataggcttt
     4201 tttattttga taaaaataat gaaaggaagg aaaactcatg aattgtaaag aaatcgaaaa
     4261 tagaaaaaaa gtgtcgaaag aaatggaaga aaagttacta aaaacaatga aacaaaaaca
     4321 atgaaacaaa aacaatgaaa caaaaacacc ttaaaagatt atctgtcgcg caatatatca
     4381 atgatatgca gttaacagga aaagagaaag cttgtttgtt aggttcaatg aaaaactttg
     4441 aacagttgag acgaacttat gtgaaaaaaa gttctaattg tcaactattg ttagaagtct
     4501 catgacattc attaatagaa ttcattttta aaactttttc tatggaaatg agaaaaagtt
     4561 tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg ttttttagga
     4621 gtcgctttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa acatgaagga
     4681 gatgtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa tgcacacgca
     4741 aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg gcaaatgttg
     4801 tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc acaaattcag
     4861 attcctgcac ttggtttagc attgccgatt tttaaagggg caaatcaata cactttgagt
     4921 cttggagcgg ctacttattt ttatgaggat gccgaaatgg gcaaaggaaa ctatgttcta
     4981 gcaggacaca acatggaaat gccaggtgtt ttattttcag atattcaaaa gttgtcatta
     5041 ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt aacgtgtaaa
     5101 tttatcgtat ctgagtactt taaattaatt gatggcgttc ctgaagagaa ctctttcttg
     5161 tctttaccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt ttatacgtca
     5221 caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttg taatcaacgc
     5281 gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt atgtaaaaaa
     5341 agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg gatatcgagc
     5401 atatgcagaa gatgtcacgc aaaaaacgcc accagagaag gtcaatatca cggttcataa
     5461 attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca aaaacgatgg
     5521 ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag attatggtga
     5581 tgttgagttt acgttaggaa atattacgga tcaagtatta ccgactgaag attctgatct
     5641 aaccaacgcg aaagtggacg aaatcgttaa agatgtagaa gacaagggat ctaacagtga
     5701 gtacgtcaag aatgcaacca ataaaattac ttcagctgta gatgaaaatg gtgagatcac
     5761 ttttgctgat caaccagcct atgtcaattc caaagggaat gtatatgtgg tttatgaaag
     5821 taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga ttgcgccaat
     5881 gactgataat acaggttcag gttttttaaa agacattcac ttatatccta aaaatattgt
     5941 ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcacagctc aaagcaaaca
     6001 aacaccttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa aaggaacaaa
     6061 attaggagat cttgtatcgg atgatcaagg aaagctgact gctactgatt tgacgttagg
     6121 aaaatactat tttgtggaag ttccttcaga agttgtagtg ggatcagata aagaaccaac
     6181 agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata aattaacctt
     6241 tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa actataaagc
     6301 accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt tccaaattgg
     6361 tgacgcagta aattatcaag gaacgattca tatcccaaca gacattgcag gcggagcaga
     6421 cggtatcaca gtaaacggag tgaaaagcga aacttctcct tacagtgtat ttaaatgggg
     6481 ggatacagca ggacaaggac tttcttatgt cgctgctaaa gcaaatatca aagtaacaaa
     6541 taaagatgga tctgtggtat taaaagaaaa tacagattat aaaatccaaa attcagaaaa
     6601 tggctttgtg atcgatttta tcgtgaataa tggccaagta agtgatactg tagcaagttt
     6661 acacggacaa gatcttcaaa tgacttataa catgtatgta aatgatagcg cagctgttgc
     6721 caatccatta acaaatagcg tggatttcgt ttataacaac aatccattta atcaggaaga
     6781 acaccacgaa aaaacgaaag ctgacgttgt gacttatggt gcaaaattct taaaagtcga
     6841 tagcggacta tttggaacag gaatcaaagc aacaccgctt gaaggcgcag aatttgctgc
     6901 taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag acggtgtcaa
     6961 agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag ataaagaagg
     7021 tcactttgaa atcactcgtt taactgaagg tgaatattca ttggaagaaa caaaagcgcc
     7081 tgaaaactat caaaaattaa cgaaagagat ctctttcaaa gtagacaaag attcatacaa
     7141 agaagaaaac cgaatcacga tcaaaaacaa tcaaaaagtt tccgtaccaa tgactggtag
     7201 caacggtttt caaacgtatg tattaatctc ttgcttgttg ctaggagcag gggcactttc
     7261 tgcagttgtt tactttaaga agaaagccta gaaggatcaa aaccaatgat tatcaggaag
     7321 cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact ttcagctgta
     7381 ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca aaatcagcag
     7441 attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt ggatgttgct
     7501 aagattgagt taggcgatcc tgttggtatt cttacaattc caagtatttc tttaaaacta
     7561 ccaatttatg acggaacgtc cgacaaaatt ttagaaaatg gtgtaggtat tacggaagga
     7621 actggtgata ttacaggagg aaacggtaaa aatcctttaa tcgcaggtca tagtggactt
     7681 tataaagata atctttttga tgatttacct tcagtaaaaa agggagaaaa gttttatatc
     7741 aaagttgatg gtgagcagca cgcttatcaa atcgatcgga ttgaggaagt gcaaaaagac
     7801 gaactacaaa gaaactttgt cacttactta gaaccaaatc ccaatgaaga tcgagtaacg
     7861 ctaatgacat gtacgcctaa ggggatcaac acacaccgtt ttctagtgta tgggaagcga
     7921 gtaaccttta caaaatcaga attaaaagat gaagaaaata agaaacaaaa attatcatgg
     7981 aaatggcttt taggaagtac tgtttttctt tcagtcatga tcattggttc gctatttgtt
     8041 tataaaaaga aaaaataaag gagggtaacc aacatgagaa atagaaaatg gctatttgtt
     8101 tgtgcagccg ctctttttac tttcactgcc tacacacagt tgacggttgc agacagtcaa
     8161 gaaagcacgg atagcacaga acaaacaatc caaacgtcag atagcagcgc gattcaaagc
     8221 agcgatgaaa ccgtcgattc gtcaacagtt gattcttcag aagaacctaa aattgctgaa
     8281 gacgatcaaa tagtcaaaca gaccattcac gttcaaaaaa ttatttcttc cgaacaatta
     8341 gacaaccagg ggcaaaagct tgaaaaacaa gaaggagtaa atggcgcgaa gttttctgtt
     8401 tatgatgtat cggacatttt acaaaagatg gatgttaagg atctcactac tgaccaaatt
     8461 gaatcccaat taaaagatcg agtgaaaaaa ctatccagtg atcaactgaa gctaatttct
     8521 aatggagaaa caaagacaat tgatcaacaa acaggtgtct ttgaattctc ggtagaagta
     8581 caggcaaatc agaaaaaagc ttattatatt gtcaatgaat caagtcctga aaacatttct
     8641 aatagcgagg atattttgct attaacacct gtttctgata aaaatggtga attcttaaag
     8701 gatgtgtgga tttatcctaa atcagaagct tcgcaaccta aagaagaagt aaaaaaaatc
     8761 gtatctactg gcgtaaagaa aaacttcttt gagaattgtt gggattttgt tactcacttg
     8821 ttttctagag attaaggaag aaataagatg aaactgatca aattattatt attcaatctt
     8881 tcttgtttaa ccgcagtctt tttgtactgc ggtttattat tttctcaact atctattttg
     8941 ccaagctacc aactatacct aatgaggatt ttaaacatac cagctgcttc catgccgcta
     9001 gatattatgg aaaagctcat taggaatcca ttatttacta cgatcaattt gatcgttatt
     9061 gtcggaattt cactagtcat ttataaaaaa gaggggatga agggatgaat tttaaacact
     9121 cgtggacaag acggttaaaa tatgtttttc ccataatgat gatgttaggt acattgtttg
     9181 ggttaaaaac agccaatgtc tttgctgaaa aagttattgt cgatccgtca aatccaatcc
     9241 agaacgtaaa gaataatttt attattccaa aagatgttcc taatgcaaaa acaaatatta
     9301 ctgtaaacgg agcgtcaaaa gtttacaaat atcatgttga tgccaacaaa ggtggtttta
     9361 cagacgacta cgtaggatta gtggagggaa acaaaaatat acgctatcct tctttccaaa
     9421 aagaatacgg tgaaacaaac cttgttttag aaggcgtaac aaccaacacc aaagtcaata
     9481 ttgactatgg aaaaatcggt acgtataacg ggaaaaaagt caatatcaag ctagttctat
     9541 ctaatattca cttatactcg gatacgttgc cgtggaatct actagataac aactatacaa
     9601 aaactcattt tcgagacgat ggctataaaa atacgaatgg tgcaatgagt aaatctaaaa
     9661 aaagaacagt actttggatc tcagacaatc ttttctcagg aattgtttac cattcaacac
     9721 aaatgaatgt gcagctagta gccacatatg aggacggtag ccccgttcaa ttttcaggag
     9781 atacatttat cagttttaac tctttgaacc ctgcaggagg aaaaagcacg gatcttaaag
     9841 gagaatatgc ccactatgac aaaatgaata agacagattg gtatgtcaga aaagatacgg
     9901 ttctttcaga atttaaatct ttctacaaca atctgaacgt ggtaggtggc cacccaggag
     9961 gaagctctaa gctgacacaa gcggataatg atttcaataa tttgcatgat aaattaggag
    10021 atccgaagtt tggtcaaggt actgtgtcct ttaaaatatc tgaagcaaat ccgacgtttg
    10081 tgattggttc ttctaatgtt caaacctggt ttactttatc ttctgcgaca atctttagtg
    10141 ttgttcctga tcaacctgaa aaaacagggg ttgataaaaa tggaaataac gtgaacgata
    10201 acatgttgca agtaggagac acgattcaat atcgaatcaa acagaaggtg aatcgattag
    10261 gagtggattt actagcggtt tacgatcgat ttgagttaat cgataattta ccaaaagaac
    10321 taaattacgt cgatgcacac gttgaaagtg gtacaaataa aaaattcgat gtatctggtg
    10381 aagtgaccta tgacaaaaca aaacaccagg taaaatatgc agcgaaagct gatacattaa
    10441 aaaatcgaat gaagtataac ggcgaaactt acgaattagt gatcaatgta aaagtcaatg
    10501 aattggccaa tcaaaatagt gtagccaaaa atcagggaac gtcgatcatt aataaagtag
    10561 aaaaggatac aaatatcatt accgtttatt tccctaaaat tccagtgaaa gaagtacaac
    10621 aaaatggaaa agatgtaaac ggacgaaatg acggtaagaa gggtacgccg acagctcctt
    10681 taaacgcagg atctgaagta caatatctag tcacacaaaa atggcacaca aaaggcgtag
    10741 acgctgcgtc agatcactac aaacaattta gtattcaaga tccaattgaa gctcgtttga
    10801 catataaaga aggttctgcg caagtaatag ataagagtaa aggaaaagat attacttctg
    10861 aaggcacact aacctatgat agcaattcaa gaacgctgaa gtgggaagct tccgctgact
    10921 tcttaagtaa caatctttta gatggacgag aaattcagct gatatttaca gctaaaactc
    10981 cattacaatc agaaaaaaat attgataacc aagccgtagt agcagtagat aatgtttcta
    11041 ataaaacaaa tgttgtgaca attggggttg atcctaactt gccacaggtc attgttccta
    11101 aaacaggttc tacacattta gtaacgattt cagcggtcag cttactatta cttgtgttag
    11161 caacttttag ttatgcggtt ctgagataca tttaaaataa aaaaacgaaa gaatgaataa
    11221 aatgaaaaag ttaataatag tagttacttc cttgattgcc atgattgtta ttttagtcgg
    11281 atgtagtaat gaaaaaaata aacaaactaa tcaagataat ggcgtattga aatcaggaac
    11341 tatgtggaaa gaagaagtag gtggtcttgt ttataatttg aaaattatag acgagactac
    11401 ctgggaatac tctgagagtg tatggcatcc agatcctgta caaataacag tcaagaggca
    11461 aaaagactat aaaggtttag aaagatacaa aatagtagat tcagctggtg ttagggaatt
    11521 tataaacaaa tctgattctt tatttattgt tgttccctat gagaagaatg gcgtaaaaaa
    11581 aataattttt cttggatcta gcaaggatga aaagcgaacg aaagagaaat tgattcacga
    11641 tggatcacaa tccaataaat ataagttaca aaagactagc gagtgattgc tagtcttttt
    11701 atttgtcctg aaggaggaat cacagtggca aaagctaaaa cgccatttga aaaagccgcg
    11761 tctaaaatct tagaacgccg cggtgtatct gaaacacagt ggaaacaaga agtccttgaa
    11821 aaagcacaat ttgacctatt acggggaaaa gacaaggact tagaaggata tatctatcaa
    11881 cgtgaacgtg aaaagcttgt tttcgacgaa attattaaac actaaaagga gcgaacaatt
    11941 ttatgtttga atcaattaca atgaattttt taacacaacc gaaagtagta gctgcggttg
    12001 atatttttag agcattacaa actgccgcaa ctagtttaaa aaacaatctg atgatttttg
    12061 ctgctgctgc ggccgttgct tccattgtta ttgggggtct tttcttccta tttggacgaa
    12121 gaggagctga aaatgggaag atgcacgtag gctatgtatt gatgggggta ggagttattt
    12181 tagcagcagc ttctattgtg gtttcgttat ttggattgtt tggacaaaca cctcaaggat
    12241 tataagaaag taaaaaagta ggagtaagaa gtagtcgata ctttttactc cttttttatg
    12301 aaaggagaat actcatgtac tttacgattg caggcaatac caaaacagat aaagagttaa
    12361 taaaaaaagc aacgaaggaa gtaggaaagc gtacttcttt tttaccgaaa acaattaaat
    12421 attctgtcat tgaaacggaa gaacagctac atgtgttagg gaaagtactg gaacacgaaa
    12481 gtcgatcttc tgttaagttg actctcacgt tacatgacac agaaggaaaa atagagaaag
    12541 ctggtacaga atacgttttt ggcccactgg attatcaaat ttcagaaggt ttctatccta
    12601 cattgatgga tttaatccgc cttgatgtgt ttaaagaaac ggatgaaact atgagtgtgg
    12661 aagagtctta tcgaaacgaa aaacttttac aagttttaga agaagagtta gagagcgacc
    12721 aaagaaaagt gaatcaaggg agtgaagtcg aagtaaatga agaagtacca tgggaacgac
    12781 caatatttcc aaagaaggag tcaaaagaaa aagagacagt ctatgttgac gaacaagctg
    12841 atattccgac tcagaaatcg atatttgagg aagagaacga tgtgatgggt caattggagg
    12901 tagatccctt tgaagaaaca atggaggatg aagagacggc cgaagtagaa gttgatccag
    12961 ttgaagctaa tgaagagtca acatggactg aaggggattc tcaggaatat attgaaccac
    13021 ttatcagtga agaagtagaa aaaaacgaaa tagagataca aaaggcaact gtatttgatt
    13081 atattttgga tcaatatccg ccagagcatg aatggatgaa agaaaaaaac aaattatttt
    13141 taaatgagct ttattcgaaa tatcaattac cccaaaccta tcaagcattt ttagagagta
    13201 aagaagaatt aatctcacag ggtaaaggca cacttttaga acagttagag tctgtccaat
    13261 caacaaattg gcatgagctt gcacaagaag atttattagc cacctttgaa gaacgtcaac
    13321 aagaaacaga tcaagaaatt aatgagtatg cgacggaaca gtcgtctaat tgggagaaag
    13381 caaaagagga actaaataaa gaagaacagc gagtgattga agaagaagta caaaaaatta
    13441 aagagcgata cgaaaagaaa cgcgacaatg cgtattcctt gtgtgtagca aagatcaata
    13501 attttactca gacgaatcaa gaagcgttat cgaaagaaaa agaaaacttg ttaaaagaac
    13561 gtattaaaga acgaaaagaa gaattttacc gaggtttgcg tacagataag ctaaagatta
    13621 gcaaagaatt aaatgaacaa ctgaataagc tatatcaaac aacgactgaa gttcttatgg
    13681 aaaaacacga acagatccag ctagaattac aagtacaaat tccaaaatgg aaaaaagagt
    13741 atgaaatcca acaacaagaa ttgaaagaac aagaagaaaa acaacggcaa aaagaaaaag
    13801 agcgcgaaca attagaacta aaaaaacgtg aacaggatct tgcggaacaa caaatggcaa
    13861 tagaaaaaca acggttaaac caagaaaagg aaaaagaagc agctgctcgt aaggaacgtc
    13921 aagaacaaat ggatacattg cgacaagctc agttaaatta tctagcacaa cctcaaccaa
    13981 cgccttcagt agctgctgtt caaaccccaa tgaacaacca aacaaatcta actccttcac
    14041 cacagcgttc ctttaacgga tggatggtgg gatgtattgc aagtttaagc ttgcttctag
    14101 gtggagggac agtatttgct tttaatcata tcaatcaatc ttcacaggct gattctgtag
    14161 ccacaacggc gtcaatcaag gaagaagccc aagaacaagc aagaaaagag tatgaacaac
    14221 aattggaaga attattgaat cgtaccaatc agtcaacaac agagtcaagt atggaaaata
    14281 gcaaagagag tgatcctagt aaggctaaag aaggatcaag cgattcaact gtacaatcaa
    14341 acacttccga aagttcagaa tcaaatgagg tgaaataatg cgtgttagag aaacaacgag
    14401 acgaaaagac aaacgattca atttagaaaa gaataggtat ctttctcatt tctatcgcta
    14461 tactccttca ggagaagagc taccagaaaa acatgacaaa cgtatttctc aaaagatttt
    14521 ttatagtggc ttagcttatc ttcctttaaa ctatgttgtt acaagaatac cagaagcatt
    14581 tcatcgattt gagattattc caggaagttt acaaggagtg aatatttttc agttacaaga
    14641 aatgccgcag ttattccatt ttgattcaac tggtttttgg aatcctttga ccccaacaac
    14701 aagtttaggc acacgtttag ttgtttctac gatttgtgtt gttccagctg tacttatggg
    14761 attgaaaatg gacatgtggt ttcgtcctct agcagatggg caaaaaggag ataaccgatt
    14821 aaaaacgtta aaggaaatcc aacaaattta tccgcaaata ccagaatcaa agggaggttt
    14881 tcctggttat ggcggaatac caattactca ttataaaaag tactggtata ttcagacgga
    14941 tacagtaaat acttgtattg ttggtacttc ccgttcaggg aaaggacaga tagaagtttt
    15001 agcaaccatt gacaatttgt ctcgtgcaga aagacaaagt tcaatggttg ttaatgatcc
    15061 aaaaacagaa ttatatgttg ctagtaaaga tgaactagaa gctagagggt atgatgtgta
    15121 tgcatttaat atcaccgatc cactgcaatc aatgagtgat aatccattgg cactcatcgt
    15181 gaaatactgg aagcgaggag acatagacac agcgacacag ttaaccaata ctttcacgaa
    15241 tacgatttat tatgatgcca acgcttcaga caataaatac tttaacgaaa atgcgcaaaa
    15301 agctgtcaat gcgcttattt ttgcgttatt ggaacaagcg gatcagacag gggattactc
    15361 caaattaaca ccgaataatc tcgtcgaact tctttctgag attggtggtt ttaattacca
    15421 agatcctgat aatgaattca agcagctcaa tgctttagat gaatttatga atcagttacc
    15481 acctggaaac atagcaaaaa aacaatatgg ggcaacaaaa attggttctg ataaagcaaa
    15541 agggaatatt ttatcgacgg caatcacagg attgaatcca tttaccctaa cgaaaattgc
    15601 gaaaatgact tctcaatcaa ccattgatta taagtccatt ggttttccga agtatttgga
    15661 tctcaaacta cacgagagtc tgttaaatca gcgaattgtt gtagagtttt atcatcaagg
    15721 ggaaaaattt catgaggaac gtgtcaaagt aggttataga ggtttttgtg aaatcaactt
    15781 tgattgtcat ttagagcagg gagatcatgt ttgcttaaaa tacgatcatc aaggaaaaga
    15841 atatgttgct tggtatgagt tttctcaaag agtattgaaa gacgaagagg gaaatatcgt
    15901 ttatcgaaaa aaacgaggag aaacccattt accagaatta gaaaaagaag ctgttctaac
    15961 attagatgag gataaaagta acttattagt agagtctatt cggatgcact ataccgataa
    16021 accaatggct atttttatgc tcacacctga ttatgatcca tctaaccata tcattgttag
    16081 tatttttctt agtcagttgt ataaagaatt atctacgcaa tgtgttaaga ctaaaggaga
    16141 taaatgtcac cgtaggattc attttatttt agatgaattt gggaatatgc caccaatgga
    16201 taatatggaa ggaatcatga cagtcacagc tggtagaagc atgttgtggg atctgtttat
    16261 tcaatcctat cagcaacttt atgcgaagta tggggaacaa aacgcagcga ttatcaaaga
    16321 aaattgtcag acacatattt atattatgtc gacagacgat aataccatcg aagaattttc
    16381 caaaaaagtg gggaataaga cggttgaaca agagaacagt accaccaatt cattaggtat
    16441 gaatactcat atcaatcgca atgttgattc agatcgtatc ttaactcctg aaagaatttc
    16501 tactttttta gaaggtgaaa cgattgtact cgctccttta aaacgacaag atttgaaagg
    16561 tcataaaata cgtccgtatc ctatttttaa tcatggggaa acgaatcttc cttatgctta
    16621 tcaatatatt gaagatttca atccatcgtg ggatatcaat gatatagaca tacattgtac
    16681 acatgcggac ttggatctta aggctttaca aattgattac gattattttc tacctgatat
    16741 ctatgcacgt cgactttatc acaatatgca cgatcaaata gaagaggaag ctaaagaaaa
    16801 catttttatt aaaatcgttc aatctttaga caactcaacg gcacaaaagt tacttattca
    16861 attttatgaa gagaagcaaa tagatcgttt gcaagaatac cttgaaaaat tgcatcaaaa
    16921 tgaagaaatt acagaggtta taaggaaacg acttttgcta gaagctgaaa aggcggtgaa
    16981 agaatgatca ttggaggaat attttccggc attgccgact ggtggtcagg cttatttggg
    17041 gatggggatg cagatcccaa aaccgtaacg aaagtcttac aagattatgg taattatctg
    17101 cagtatcaag atgtattcag ttggatttgt aatacttttt tatggggttt gattaaaggg
    17161 ttatataatc tcaatacttt actagaaaaa acaatttatc aaagctttga tttaaaagac
    17221 ttattgaacg ctgctggtgt taatgaactg tttcaaagtt tgattagtaa ggtattggct
    17281 cttttttgtg tcgtcacatt aatttattta ggcttaaaat tttttatgag taaacatcca
    17341 ccaaaaatca aaaatatctt ggttaatcta tttatggcca tgattttgat ttttggtggt
    17401 agttctttaa tcgatgaagg gctaaatatt agtaaaaact tttacggaga cgtgactgca
    17461 gcaaataaag cagataaaac tggatcacct gcttttcaaa ttattcaaaa taatgtgatc
    17521 gatgtgagta cgctactaga aactgatccg aataaagtag aatccctacc aacagataaa
    17581 agaaatgctc tcacggcgaa aaactttaag acagcaaata ttaattcagt gattattcct
    17641 aaggaagccg ctaagatggc ggatgaagat aagagtgggc tatcagatat acaaaaggat
    17701 cggctaagag cgttacaata ccgactggaa ctggatgata atggaaaaga aatccctgta
    17761 gagattagtg atgaggggtt agcaaaatat gtgtatactt caggctatcg ccgatacatt
    17821 agtagtccag ggattatttt agctggtgaa tcttctttgt cggttgccta tttatttatc
    17881 ttgtttacga ttgtaacttg cattattgag ctggtattta aaaaattcta tttagtaatt
    17941 gccgcgtcaa cggactttga gacaggacaa cggatgaaaa gtgctataga agatatttct
    18001 caatccttct tgttattggc gtttacgatt ttagaattac gaatctatac attaatgctg
    18061 tcaggtatcg gtgatttgca cgcagcaggc aagatcaacg gatttttgta tgttgtcgct
    18121 ttaattgtac taaccattgc gctattcaaa ggatctcaat ctgttacgaa gattttcggt
    18181 gttgatacaa gtctgaaaaa cggcagcaat tctcttctta gcatgtttgc cttaggaaat
    18241 attgcgaaaa atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa gagtggttta
    18301 agcaatttaa atgatattcg taaaaatgga tttggcaatc gaaaagatgt tacccttgat
    18361 aacggggaag aacaatcaga cgataaagct acaactgtac ctaatagtaa gaaaacagtt
    18421 tccgaaatgt tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg tcttggttac
    18481 gtcaatgaac gtgggttaaa aggaacagct gaagatctta cagaaaaagc caaagacacc
    18541 ttagaagaga aattagacgg cacgagagct aaagaattgg ctgaagctgt gaagaatcca
    18601 tccgaagcac tagaaaaaca aatagaaaaa gcttcagaag tcaaagaagg actaaaaggt
    18661 gcgtatggtg aaggacaagt caatgcagca gtaaaagcta atacaacaac cgaaaaagaa
    18721 aatcaagaag gtgcagcgat tccttcagcc aaagatcacc aagatgagtt gcattctaac
    18781 gaaaattcag aagaaggaca aaatggtcaa ttaccaaaaa cagatccaga acaatatcgt
    18841 tataaatcta acattaattc tgaaaatgaa aagcacattt cagatttacc aaaacgaaat
    18901 caagaagtac aaacggatga ggattccatc gatctgccaa atgataatgg agtcgaagtt
    18961 ggaactgaaa aatcaacgaa aattggttta cctaaggcac aaaatttacc aatgaaagat
    19021 agtcgtttga atcaattcaa ggagaatgat caaagctttg ttcaccaatc aactgcaggt
    19081 actgttcaaa tgaaatcaag tcgtttgcct gaaaatggga atttgacaac tatttcttac
    19141 aatttatctg aaagtggtga gacagagaat gcgtccgttc aaaatattgt gacaagagaa
    19201 gctgcaccaa aacctattca aaacgttcat acaggttaca agccagcaaa tgtggcattt
    19261 actgcaccaa caaaaataaa tgtaacaaga gaaactaaaa acaggaacaa tgaattgaaa
    19321 gaacaactat tgcgggatat caaacaggat atcaaaccat aaaaggccta caagttaggc
    19381 cttttttcta tgtatgaaaa ggagaaatca tttatggata aatatattaa catgaaaaat
    19441 cctccaagat ttagtgggga atggaagttt ttcgcctttt tctttttaga tctattaacg
    19501 cttggtggtt gctttttttg ggcgaaaata gtcaataatc ttgtccactt accactcttt
    19561 tttgcactct tgaattatgg cttatggatt gcgatagctc tagtgattgt ttggcgaccc
    19621 tataataacc caggtcaacg ccagattttt gcgattatcg aatctatttt ttttacggac
    19681 aagaaccatt accatgccct agatcctaac cgaaattatc atggtacgta taaggaggat
    19741 gaataaatgc gagtcaaagg acaaaactct gttgataaga aatccatttt tcagtttgga
    19801 aaaccagaag aacgatcgat cttggattta tttccttata aatcctgtac agaagagggg
    19861 gtcttagtta ctaaagaaga tcattttcaa cgttattatc gtgtagtttc cactgacgtg
    19921 gagggattaa atgaacaaga gaaactggaa cgaatgaatc agttgaccac cgtcatgcgt
    19981 acgtatgtgc caacaattaa gttagttagt ttgaccacag aaacggatct aacggagcaa
    20041 atcgtacaaa agcggcagtt attagataag aatcgtatgg aacaatcctt aggaagaaat
    20101 ctacagcgat taagaaaata tgaaaagaaa ttagtagagg aaatccagga attaaaacga
    20161 gcagaacaag aacgtcctga cttaagcttc tttttcatca ttgaaggaaa gacaatcaag
    20221 gatttacaaa ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca
    20281 ctctacaaaa aagaattgat caagattctt tatcgaatga ataatatgaa tgatgagtag
    20341 gaatggagaa gagagaatga aatatctgtt gatatggtta gaaaaagatg ttccgaagga
    20401 agagaccact agaaaatttc ctcacacttt aagcctgggt aagttgggaa aagggcaatc
    20461 ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcgattaaa
    20521 acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca actaggaaat
    20581 cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa acaagtgatg
    20641 ccacaaggtg gattaaattt cgatgaagag tatgtgacct atggaaatgg ttattcacgg
    20701 tgtgtgacag tttatcacta cacaaaaaat ccaacgcttt tttggttggc aaatctcatg
    20761 aacaatcctc atacagtggc cacactagat gttagcacag atgaaaagca aaaagtgatt
    20821 gatcaaatca accgcgcatt agatgaatta aatgatcgat cagataatga acgaaaagcg
    20881 actgaccgaa ataaaagtgc ggaagaatac ctggaactag cggaatatgc ccgaaagttg
    20941 acttctaatg gggaaatttc taaacaagtc aaagtgagag tacatgttta cggaaatacg
    21001 atccaagaag tggatgaacg ggcgcaggaa ctgatcgaaa atctaaaagg gatggattat
    21061 cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt taccatgttc
    21121 gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc gttaaacatt
    21181 ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggaggtac acaatatggg
    21241 gaaaccatga caggcggccc atttgtctgg aacgcgacgt ataaaaatag tgtacggttg
    21301 tctactaata ccttgcttct aggaatgatg ggagctggta aatcaaccgt tcttaaaatg
    21361 atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac
    21421 tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg atctgacggg
    21481 atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcttc cagtttatat
    21541 gacgatggat cagtcaaaga tttgaagatc aatgaagcag catcctatca aacgcactta
    21601 gacaaagtag tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca
    21661 gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc acgcggcttt
    21721 acttcctcaa attcgcgttc aaatactgat acgcaagtca caggtaaaga tccagaagct
    21781 tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc
    21841 caagagaaaa agaatagaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg
    21901 tatggcatga tttttgatgg tcatagtacg attcgacacc ttgaccaaca acagttggtc
    21961 ttttttgaca ttgactctat tacaggctta gatcgagggg tacaacaatg tcaaatgtac
    22021 atggctatga cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgacgttta
    22081 attaaagaaa aacaaatgga agaacaagac ttcaaacgct tcttggcgat gattgatgag
    22141 tgccataatg cgattaatcc agattttatt gaaacggtaa attatgtcac aaactttcaa
    22201 cgagaaatgc gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg
    22261 gtgccagaaa atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct
    22321 tctacgaaga tctttatgcg tatggatgaa tctattttac gtcatataga aggattagtt
    22381 ggtaaatcaa tgacgaactc agaactagaa tccattcctc aacaaaatca aggagacgct
    22441 gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa
    22501 ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaagcagt
    22561 atgttaagcg tcgtgcaaag cgacgcttat ttttgtggtt gtttggtact tctgcaggtt
    22621 tgattactat tttgatcact gtgtacgtga ccctttttct aatactggcg gcgggttcga
    22681 ttgataattc tgattcagat tcaagcagtg gtggcgaagc ttttggcgaa gcttttacag
    22741 gagaatattc ggaaggatta cccatttata aagaaatcaa aggaagaggc cctttttctg
    22801 atgagattgc gcaatatgct gtaggtgcag cagtgaaata taaattatta ccaagtgtga
    22861 tcctttcgca atacggttac gaatcagcgt ttggtacgtc ggcgtccgca agaaatgatc
    22921 tcaactattt tgggattacg tggttcgatg gctgtttgtt tcctaaagga actgcacgag
    22981 gaattggtgg tattgaaggt ggctggtaca tgaagtttcc caactcaaaa gctgcattct
    23041 cctattacgg ctttatggta gccacacaat caaattttaa tgcatgtgta ggaaataaaa
    23101 gccctggcgc aagcttattg atcttaggac gaggtggtta tgccgctgca ggaattactg
    23161 aagatagtcc gtattataca gggtgtatgt caattattac ttctaataag ttaaccgaat
    23221 atgatgaatt cgcgatcaaa cattggggtg aaggtaacaa taacaatggc acaattactg
    23281 gtgaatggac gaatccattc cctggcagca gtttagataa aagttccttt agcggtgggc
    23341 aattatttgg aacgaatcca gggggagagt tccgaccaaa tggtttccat gacggattag
    23401 attttggttc agtcgatcat ccaggaagtg aaattcacgc ggtgcatgga ggaaaagtcg
    23461 tgtacgttgg taatcctggt atttcaggtt taggtgcatg tgtgatcgtg attaattacg
    23521 acggtttgaa tatggtgtat caagaatttg cgaatagtac aggaaattct cgtgtgaagg
    23581 taggcgatca agtaaaagtt ggtcaagtaa tcgggatacg agatacagca cacttgcatt
    23641 tagggttcac tcgcatggat tggcgtcaag cacaaggaca cgcattcaca gatgatggta
    23701 catggattga tccattacca tttttgaata gcagcaaaaa atagatcagg agaaaaaata
    23761 tggaaaataa aactttaaaa atcgtcttaa cagctgcaat tagcgttagt ttaatcggca
    23821 atatcgcgtt gttttggcag cttaaagaag aacaagagac aaataccttt aaaattgaag
    23881 aagtggctaa aaacgcgtct gagaagctca aaaaaaattc ctctgatgca caggtgaaga
    23941 tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga aatcgatcag ctgaagcaac
    24001 aagatggcac aaaagcaaaa gaggaagtca ctgaagcaca aagaaagacg gcagaagagt
    24061 tcggtcaatt ggccattgat aaatcattaa gcagaaatga attaacggaa aaactaaaag
    24121 atgtcgcaac ccaagaagtg atcgacaaat taagtcctgc agacgatgat caccaacatg
    24181 atgattacgg aagttactct tttacattag gtgacgtgca aacttacgct caaacaagca
    24241 gtgtaaaaga ggaacaaaat tttgtggtat ttgttgatta tacaatcggc aatcctcaat
    24301 tcaaagatgt caaaccgcaa aaaataaaag gtggattaac tgtcactgaa aaacaagtag
    24361 atggtcaatg gaaagtcaca gactttagtt atttcactcg ttaggaggaa gaaaatgagt
    24421 aagaaaaaag gagcaagacc acgtttcaaa aaacggaaaa gttatcttat atggctggga
    24481 atctttatga ttctcggctt tttaattctt gctagtccga tttatacgca taagaaggta
    24541 gacataccag atgaggtaag cacgaacgca tttttaaaca cgaaggaact gacgatggtt
    24601 tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat ttgcgatcga cgatgaagat
    24661 caaagcttat taaaagaact ggctaatctt aattatcgcg tagaagtaaa gacagctaaa
    24721 ggggattatc aagcaataaa agccaaagtg attaaggtgt ctgatgatta ttttgcagtg
    24781 aagctatcgc atgtaccaga agaatatatt gctatgcgat taactattat tcctgaaaaa
    24841 attgatccaa aggtggatat gcaagaaccg caagatctga ttttttatat tcacgaagat
    24901 aaggtcaaag atcacgtgaa agacgaagat tatgaacaac atgcgatcaa ttacaaagtg
    24961 aaaggataca agaaagaaca aaaagcagca caaaaacaaa ttgaatcctt ccaagcaacc
    25021 attgatttga acgaagaatt agtgaagaaa ctaaaagatc aattgcctta tcaagtcgca
    25081 gacgatcagg aaaatactga aaataagatt aatagttacc agcaagaaat tgaaacatcg
    25141 aaaacgcaaa tgaaagatca agaagagagc attcaaaaac ttcaagaaaa aattgatcgt
    25201 atgacaaaag aaaatattta gaagaatagg tggagtaaaa gaaatgaagc gagcgaactt
    25261 ggtcggcaaa gatactttcg ctgagcagaa agtattgatt aaagcctatc tcaaagttgt
    25321 aaaagctaaa gaaatatgtc aagaagtgaa tttgtctaac acaacaatat ctgagttgaa
    25381 gaatggtaga aagagtttag ataccttaaa attcagatcc tttgaagctc tatatatagc
    25441 tgctaaaaaa tattttacag ctgataacaa ataaaaaaca ggagtatttt atgtgaaaaa
    25501 agtatataaa aaatgcttat gaatcagcta tatcacctta accatttgtt aatgagatgg
    25561 gagaaaacat taaccacgat tctaacgcaa acatcaagca cataaaacat ttaacactcc
    25621 tcctttctgg ctgaagattt caaccaaaca ggaagacagc gtatcaatta tagcatacac
    25681 tttttcttaa attcattttt catatgacaa aatatttcct tctaatcgac aaagttagaa
    25741 ggatttttta ttgtctaaat acacaaataa ccttaattta taagagcata aggaaacgtg
    25801 ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct attacgtttc tcaaaaataa
    25861 atgtgggaac aaaatattta aaaataggat agtgaaagga tagtgaagga tagtaaaagg
    25921 agggtagtag gatagcaaaa aggataggct ccttttcgct atccttttta aattggagga
    25981 attaatatgg aagtaagaat aagaaatgtg gatgagaaaa tcgtaagaga aattgatcga
    26041 agatgtgacg aactaagtaa ccgtacgggt aataaatgga gtcgtaacga ttatttaaaa
    26101 ttactaatag aaaatgattt tgatcgtcct ttgatggaat acaaaaaaga aaagtttgat
    26161 cagttattag aaaagttttc agaaattcaa agctataaca caaagatatt agaagaatat
    26221 gttagccaaa acaatcgtat tttagaaatc ttgatcgagc aaaatagagg aagtgaactt
    26281 tgatatggca aaggttatag acaaacacat ttactttcac caaacacata tcgatgtgtt
    26341 ggatgagatt atttctactc atgcagaagt taaaaactat agtgaagccg ttcggtttct
    26401 atgtatgaat tatgatccag aaatggagaa aaaagtaaat tccaatcaag caaagttgaa
    26461 tggaatgagt aaagaaattt ctattttaac cgaaatggtt tcagagtttg cagatcttca
    26521 tttgaaagat acaggaatac taattggtac aaattcaacg gtttaccaag aagcaaaaga
    26581 acgtgtggaa ggaaaaatta aatctaatca aaccaaaaaa tattcccctc agttgaagaa
    26641 acctgtgaat gaatctgttt atgccagagt agatccattt gatccaacga agattctaaa
    26701 aagataaaag tagaagggga acatgatgaa taaatcgcct gcagtcattc tcacttgtca
    26761 atttaccttg cccaataaaa aatctttttc aacatacatt gattatatga caagagaaaa
    26821 agcgctggaa gaaatggaac gacgtacgcc tgaagaagaa caggaactgc aacgaatcaa
    26881 gaatgcttta gaggattttt atattcctga aggagaaact ttttcggaga gtaagacgga
    26941 taaagaattc agcgaaacgt cgaatgaagc tcaatctttg atgggaaatg gaatcaactt
    27001 tgacaagtta gaagaacaag attttaccaa atatctttct tatatgaccc gtcactatac
    27061 gttagaacaa aagaaagaac tgacgcaaag tgaacaaaaa gaataccaac gcttgaacca
    27121 agcaataaaa aaatatcaaa cagagccaca caaaaaagct tcaggaaacc ttcctggagt
    27181 tttttctatg gcttcagacg aagtgaaagg aagtgatctg aaggagatta agcaaatctt
    27241 ccaaagagga caacaaaaag gctcaatcat ttatcaagat gttgtctctc atgataattc
    27301 gtatcttgaa aaaataggat tgtacgatcc caaaacagat actttagatg aagaaggatt
    27361 aaaagcagct ggaagaaaaa tgatggagac attattcgag aaagagcaac tgaacgaaac
    27421 aggctactgg atggcaacga ttcaccgtaa taccaaacat atccatattc attttgcagt
    27481 tgttgaaaaa gaaaatacgc gtaaacctta cacggaggtt gaaaatggtg tgagttatat
    27541 cgtaccgaaa ggtaagagaa gacaagcaac attagacgca atgaagacca attatgtaca
    27601 tgagttagaa cgtttttctt atgaaaagga tcgaatgatt ggtatggaga aacgaaattt
    27661 attgacgcgg aaaagtgatt tgcgtaacac cttaacaaaa gtggtaaaag agccagcgcg
    27721 ttacgatcga cacgcaatga acttattaaa tgaagtatat aaaaccctac ctgacaaacg
    27781 ttcagaatgg aattacgggg atgaaaaacg aaccaaactt tcaccataca caagagagaa
    27841 actcgatgag ttaaccaaat atgttttaac aaaagaacct gaatatcaag aatatataca
    27901 gatttcaaaa gcattgaaag aagaagcaaa aagtctttat ggggaaagca aaagagaatc
    27961 aaaggatgca gaaaaaaacg ccctctttga tttaaaaaag cgcacaggaa atgccatcct
    28021 aacagaactg aagagacaag atagtcaaat aaaaccatta atagaaggtg tcgatcagat
    28081 gcaatctatt tataatgaac gatccttccg aacacttaaa cagttgccaa caaatgaata
    28141 caagtatagt tacgaagaat tcatgaagcg aaggaaacaa ttccaacaaa gagttgtcac
    28201 gcaacggagt ttgcgtcaat taaatcgaac gattaatgcc catgaagaaa aataccacgc
    28261 aagtaaagca tacgaagagc aacaacgaag aattcaaatg gaacaagaaa gacagtagga
    28321 ggaatagcaa tgaaacaata tttagatcaa tggaaagtta tagaaggatc attaagagaa
    28381 gaacgaatcg aacaacttcc cgattgttta gaaaaagagc atttgtttca gataagagaa
    28441 atgttgagaa acgaacagtt tgatccaaat caatttttag tcgttgagta cccagctact
    28501 ggtgtgtact gttgtaatca tgtgaagggt gaaaaatatt ttatcattca agaatatgaa
    28561 ggcaagttag cgccttatta tactacttgg gaaatgaatg aagaggggat caataacttc
    28621 ccgtgtaaaa gcattgaaga gtctatttca ctcacagaat gttagttgtt aagtgaataa
    28681 aagaagtaaa agagtcctga acgtaattgt ccaggagtct ttttttcaat gaattgtttt
    28741 ccgccaattc aatgagtggt gcgatttccg actacattat atcaaaagaa agagaaggga
    28801 ggaagaagaa atggcttatt caactattgg agttgttgtt gaaaaaagca gagacaatct
    28861 cgtttttgtg acagaaattc aaacaggtag atcttttgtc gtcacagaca aagcggcaaa
    28921 agcttatcga agtggcgata ttttagcgtt aaatctgatt acaaaaacgt tcgttgatgc
    28981 agtagaaaat tatccatttg tttaggagga aacggacatg gcagaagaaa aagaaccact
    29041 agacaatagt cgtttgggaa aatccaaaag aaaattagca cgattgcaaa atgaattaaa
    29101 tgatcaaatc gaaaagatgt ttgaacacca aagaaagacc aacggtcaac caatgaacga
    29161 taagagaaac gggcattctt ggtttaggca acaagaacga atcgaaaata aagtccaaag
    29221 tctgagagaa gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga
    29281 gctaaaggaa atgggttata acaagtacgg tggcctggat atgacgatcg aaaatattcc
    29341 aagaatcaaa gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagctgccac
    29401 gatccgaaaa taccaacgaa aattggaaac attagaacag ctgaaagaaa aatcagagaa
    29461 aggaaaagaa aatattcttc ctgaagtaca agcgatcatt gacagcggac gagtgacgca
    29521 atggaaaaag aatccaacta tctattttct gaaaggctat cgaaaagtcg ctttagaact
    29581 caatgaaaac gggggatttg aggaatctaa aaaataccaa gccacgtcgg aagaagaaaa
    29641 ggcagctgtt cgtgaattac ttgaagaagc tacacctact gattctgtga aggagaaaga
    29701 aaccgatcat ttctatcgag tagaattcaa cgaaaatgat ccagcaaaag ggttgaaaag
    29761 ttatggtggg gagatcgtaa cactagaatt gatcgatcaa ctcaaaacac tggataacca
    29821 atacgccgca gaagcaggct attataaatt ctattttgaa gaagtgaaga atggagaagt
    29881 agtagatcag atacgaatgg atctagggga tggcgtcaat agtaatcacc ccatctattt
    29941 agagttagct agattgtgcg aacaagtacc tgagaaggaa caaaaacaaa cagtcaaaaa
    30001 tggacctatt tcaaaagtac aaaatgagat taaagagaca aaaacaaaac aaaagggtta
    30061 tcaaaaacga acggcagaag agattaagca agaagtcaaa tcactttccc aacaggcgct
    30121 tgatgcggtg aaaaagcata ctcattcacc tgaagacgtc aaagaacttt tgaattttat
    30181 gtctcgtttt ccagaacgat cctttagaaa tcaattattg atcgaggaac aatttccagg
    30241 cgcaacggct tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg
    30301 ggaacaaggg aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg
    30361 tggctttgtt cgagaaacag aggctacacc agaagataaa aaacgaatcg aaagtggtca
    30421 aatcgaagtg atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac
    30481 acaattgaag cctgaagact atccgaaaat cttcccgaac cgcgtctttg attttcaatt
    30541 ggatcaaaca gggcatacag aacttcaagt tgggattcaa gcggtagcca aaaatattgg
    30601 aattcaaatt cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg
    30661 tggggcgtac gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa
    30721 tactcctacg cagcatttag ccacaagtat tcacgaatta gcccatgcta gaatgcacaa
    30781 attttcagaa ttagatactg caacaaaaga actacaggca gaaatgacct cctatattgt
    30841 gtgcaagcat tttgggatgg acacctcgga gaaagctatt ccttatattg cgacatggac
    30901 gaagaatggt cagacgttgg atgataagga agctgcggaa cgtgggaaaa tcatgaatga
    30961 tgtcagtcgt gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag
    31021 agaaagagaa ccagagatcc agccagcaga agtgaagcag gatgagaaaa atgttgtaga
    31081 aacagcagaa caaaagaata ttgtgctcgt tggctcactt tggatcgaca agaaagacgg
    31141 gaaagtcctg gaagaagaca cgggccgaac gttacatttg aaggatgcgg cgtttattga
    31201 agatacgcag cataatcaag taattttaaa aggaaaaagt tatggaaaag agatccaaac
    31261 ggcgttacct tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgagacagt
    31321 gtcagaagtg gcaaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga
    31381 acgtgtataa aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg
    31441 aggtttgaaa atgaccgaga aaaaaatgag tcttattgat cgatgtaaac agatcgatat
    31501 agtggatttt gcaagaaata atggtttagc tgttgtgaat aaaggaaatg attatcgatt
    31561 agaggatcac ctttcttttg tttttgagcg gaaaaagcag tattttagct ggaacagtcg
    31621 taatattcat ggagatatta tcgacctagc agatctattt tttgtcgatc catcaattac
    31681 agataaaaaa gaacgcttca aagcagcaac aaactttatt ctgaaaaatg aaaataaact
    31741 cgaacgcgtg gagaatcttc attttgagac agaaaagtac aaagatcacc cgattgatta
    31801 tcaaccactc acggaaaaag gaagaagtta tctgaaggaa gaacgaaaat tgcccgattg
    31861 gctgattaat tatgcagaaa aagaaggatt aattgctgaa ctaaagccaa aacatgaacg
    31921 gcaaaatttt ttagttggag acgaccgttt agatcatgcg gtcgcttttt tttggaaaga
    31981 ttcacagaca aaagagacag taggggcaag ttatcaagga acgatcgtcg attttgaccg
    32041 tttcggtaaa cggggaacgt ataaacatat tgacaagaat cctacgccaa atcatggatt
    32101 taatttaaaa ataggcgatc cgaagcattt aaaattcttt gaaagtagta ttgatctatt
    32161 aagttacgct gcattgaatc gggagaaact acaagatgct tggttagttt cgatggacgg
    32221 gttaaaacat catgtaatca gtcattacgt tgaagagtcg atttctgaat taagtcgaaa
    32281 acaaacattc cctcaatcga ttgaagtatg tgtggacaat gatcgtgcag gacatatttt
    32341 ttatgaaaaa gaacagctga agggaatcgt tgatcctttt accaataaga aaatccgttg
    32401 tgagcgagga attccaaatg attggcaagt acctaaagaa tacaaagcta cttatgaagc
    32461 agtcgcaaaa gaaatgaatg ttgaacctga ggcaattatg gccatacaca aaactgaaac
    32521 taatttacag ctgacaaatc aattggtgtc tgcttatgat gttcaatcta cgtttggaaa
    32581 gatgttggcg aagggcgaac cagtagaaac aattgatttg aaagaagcat gtaccacggt
    32641 cgcgaaggaa ctgaaagtat gtgaacgcgc agatggaaca tacaactttg atcgttttta
    32701 tagtagaaaa gccaacataa aagacgttaa tgcaggaatc cttctttcct ataaagcaga
    32761 acaatactac aaaggctata aaaagcatga acatgaattt gtacctgaag tgaaaaagga
    32821 ttggaatgat caattgaagc atgagatcca gcaacaagaa atcagaaagc aaaaacgtgc
    32881 gatgttgttt caacaaggca tacaacacga aagggagtga aaccgtcact atgaaaaccg
    32941 tcattttagc agaaaaaccg tcacaagcga aggcttatgc cgatagcttt tctaaaacct
    33001 cccgaaaaga cagctatttt gagatccaag atcgattgtt ttcgggaaaa actgtgatca
    33061 cgtatggttt tggtcattta gttgagttag attcgccaga tatgtacgat gaaaattgga
    33121 agcaatggtc attagaacat ttgcctattt tcccaacaca ttatcgctat catgttccta
    33181 aagataagaa aaagcaattc aacgtggtga aacagcaact tcaatcggcc gacaccatta
    33241 ttattgcgac agatagcgat cgtgaagggg agctgattgc ctggtcaatc attcaacaag
    33301 caggcgccga tcatggaaag atatttaaaa gactttggat caactcctta gaaaaagaag
    33361 ccatttatca gggattccag caattacgag acgcagaaga gacctatccc aaatttgaag
    33421 aagcacaagc acgccagatc gctgattggt tgatcggcat gaacggcagc ccactgtata
    33481 gtttactctt gcagcaaaaa ggcataccag gaagcttttc cttaggtaga gtccaaactc
    33541 caacgcttta catgatttat cagctgcagg aaaagattag aaacttccaa aaagaaccat
    33601 attttgaagg aaaagcccaa gtaaccactc aaaatggcac atttggtgcg aagcttgatc
    33661 cgaatgaaac acaagcgacg caagaagcat ttgaagccta tttaaaagaa aaaggcgttc
    33721 aagtaggaaa gcaaccaggc acgattcttg aagtcgaaac cgagaaaaaa agcgcggcaa
    33781 gtccacgtct tttttctcta tccagccttc agtctaaaat gaatcagctt atgaaagcta
    33841 gtgccaaaga tactttggaa gctttgcaag gcttatatga agggaaatat ctaagctatc
    33901 cgcgaacaga taccccttat attacggaag gggaatatgc gtatctgttg gatcacttag
    33961 acgaatacaa gcacttttta aaggccgaat caatccctac tcctattcat acaccaaata
    34021 gtcgatatgt gaataataag aaagtccagg aacattacgc gatcattcct acaaaaacgg
    34081 tcatgacggc agcggctttt gaacaattaa gtccactgca gcaggccatt tatgaacaag
    34141 tgttaaaaac aacggtggcc atgtttgcgg aaaaatatac ctatgaagaa acaacgatct
    34201 tgacgcaagt tcagcagctt cagttaaagg caatcggaaa agtaccgata gatctaggtt
    34261 ggaagaagtt atttggcaaa gagagcgacg gaaaagagaa ggaagaagaa ccgctacttc
    34321 ctaaagtgat aaaaggagaa acggtgacgg tagatctaca agtccttgaa aaagaaacca
    34381 agccaccaca accttatacc gaaggtacgt tgatcactgc gatgaaaaca gctgggaaaa
    34441 ccgtggatag tgaagaagcg caatccatat tgaaagaagt agaaggaatc ggtacagaag
    34501 cgacacgggc gaatattatt gaaactttga aacaaaagga atatatcaag gtagagaaaa
    34561 ataagctcgt agtgacgaat aaagggattt tgctttgcca agccgtggaa aaagagccgc
    34621 tacttacgag cgccgaaatg accgccaaat gggaaagcta cttgttaaag atcggtgaac
    34681 gaaaaggaac acaaaccact tttctagcca atatccaaaa atttgtctct cacttattgg
    34741 aagtcgtacc agggcaaatc caaaccacta attttggatc gacgttacaa gaagtgaaag
    34801 cagccagtga aaagcaagaa gccgcgcgtc atttaggaac ttgtccaaaa tgccaggaac
    34861 aagaagtctt actctatcaa aaggtggcag cttgtacgtc tgaagcctgt gattttaaat
    34921 tatggacaac gattgccaaa aagaaactaa cagcgacaca gctgaaggag atcattcaaa
    34981 aaggccgaac aagccaacca gtgaaaggat taaaaggtca aaagggttcg tttgaagcaa
    35041 cgattgttct aaaagaagac ttcacaacga gctttgaatt ttctgaaaag aaaaaaacga
    35101 actataagaa aagaacacgc cgcactacaa aataatggag gggttatctt atgcgaaata
    35161 caacacacaa acaacaagaa atcgaaaatc aaaaagcaaa aattcgttca gaacaagaga
    35221 aattacgtgc caagaaaaga agactgcagc aattgaagaa ccagcagagg attcaaaaag
    35281 aaatcgaatt agatcgataa aatattcctg actgttgtat acgtataaac ttatgttata
    35341 ctatattata agtttgtacg tatacaaagg agggagaaaa tgacaaaagc aatggtatat
    35401 aagctgctag aagaatttaa tggaactttg gctttgaagg atgctaaaaa agcgggcatt
    35461 tctcctgtta cgatcaaacg tatggtagat cgaggggagc tggatcgaga gtatccaggt
    35521 tttttcacct tacctggtca atttcctgat gaattgttta tggcacaaaa gaaatatgag
    35581 cgcggcatta tttcgcatat caccgcattg gatttatatg atttaacgga tatgatccct
    35641 agacagatcg atctaaccgt accttacggt tatcacgttt ctgaaaaggg gttgaaggaa
    35701 ttcgcagttg agttacacta ttcaaaagcg gaatggtatg aattaggtaa gatcgaaata
    35761 aagagtcgct atgggaatcc tgttatagct tatgatccag aaagaacctt gtgtgacatt
    35821 tggaatccct ggtataacgt tgaggatgaa atcaaagtaa aagcaatcaa aaactacatg
    35881 gagtcggaca gaaaaaatct tagaaaattg aatgagtatc gacgaatttt gcctacagac
    35941 aaaacgatgc gctcttatat tatggcttta aattaaagga gaagacacat gacacctacg
    36001 caattaaaag caaaagtgaa gaatatttcc agcgaaaaag aagttgatcc gcagcttgtt
    36061 ttaagacatt ttatgatgga aaaattcttg gaaaaaatct cagagtctcc ttatcgtgaa
    36121 aattttgtgt taaaaggcgg cttcttaatt ggctcaaagt atggcattga aaatcgaaca
    36181 actaaagaca ttgatacgac tttacgggaa atgaaagtaa ccaaagaaac attgacaacg
    36241 gttctaaacg atattttttc aacgccgacg aaagaaggca ttcaatttga gattcaagga
    36301 atgaaagaaa cgagggaagc tgattattat ccagggttta gcttacgtgt actggcacat
    36361 ttggaaaaca tgcggccaga ttttaaggta gatgtgacta caggtgactc gatctatcct
    36421 gcaacgatca ctcacagtca taaattgatg tttgaggatc gaacgattcc tttggaatcc
    36481 tatcctacag aacaaattat tgccgaaaaa ttaagtgcaa cctttgattt tttaacagat
    36541 aattcgagag gaaaagattt ttatgatctc tatactattc ctaaaatgga gaagatcgat
    36601 gagaagacct tgaaagatag tgtgagaaat acatttacac gtcgaggaaa aactgatcca
    36661 ttaaagactt actacgaaaa ggatatggat gtactgaaaa atagtcacga gctaaaagat
    36721 acctggaaga aatatcaaaa aagcaatcct tatgctgcgt cgatcacgta caaagagaca
    36781 atggattcga ttagtaattt gatggaaaaa gtgattcacg tcgaagaaaa agagcgaaaa
    36841 ctagcttatc aaagacacca acagatgaat agagaaatgg agagataaag atgatcaata
    36901 atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatact gcaagtggta
    36961 ctgcagtagc gacttttacc ctagccgtca accgcaattt cacgaatcaa aacggcaatc
    37021 gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaacg atggcgacat
    37081 tagctaaaaa aggcagtttg atcggcgttg tcggacggat tcaaacacgg acttatgaca
    37141 accaacaagg acaacgtgtg tatgtgacag aagttgtgac cgataatttc caactattag
    37201 aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc agtccaagct
    37261 ccaccacctt tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac
    37321 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttagttcg
    37381 agaaagaagg agtaagcatg gcaaaaaaca ttagtctaac gatggatcta ttgatacccg
    37441 ttattgcatt tatagacggg atttctttgg gtattactga agattatttg gtaaatagaa
    37501 ttgcgaaagg atcactattt cttgtattcg tgtatcttgt ctgttcttat cttttaaaaa
    37561 gagcaggaaa ggataattcc ttttggcgat tgactaaagt cacaatagca gcgacagctg
    37621 ttatacttac ggctattcta ccgctttata tgtagggtta acgggaaatc attgaacttc
    37681 ttgcgaaata ttgctataat gaacatgaaa aagaggagtt gcgcgaacaa cccctcatta
    37741 acagcaccga ttaaagaacg gtggcagaat attagttatt tggaaataac cgctgacctg
    37801 ctaaagtaga cggttatttc tttttgtcat ttttaagcat atttacaatc aaactaatca
    37861 aagcaatggt aaacatacca aaaccaagaa tggtttgaat tgtctcatat gcggacaaaa
    37921 ggcttactcc tttcatagat tttagcactt acgttcataa gcaccaccac ctttcagaag
    37981 tcgccaccgt cttcaaactt tactgtcttg attattatag catatctaaa atcgaataaa
    38041 aattctcaat tttagctgtt tttactataa ttttagcgag gtgatcgcga tggaacatta
    38101 tactggttta ctggataaaa ttcgggtaat caagacacag ccattgcttg tacgatttac
    38161 attacgtaca aatcatgaac cgattaattg tgtggtggcc aatatagaaa tagcggataa
    38221 attgctgatt ttggaagaag gcaagtatac tatttcagtg acaggtcact tcaataaacg
    38281 gaatcaatta gtcactgaat cgatgcaaat tagaaaccct gatcatttta cgagattaat
    38341 gggcatataa ataaaagaag agctgcaatc ctaatgtagg actgcagctc tttttgtttt
    38401 acatagaaac aggaagaaag gaaaaggcct gtttaggaag gcacaagcgc cttctcaatt
    38461 gaaaaacctt tctctcactc caaccgccac cgccgttcga gaagaaagga agtgaacgtt
    38521 gcacgttcac taagataagg ggtcttgctt tgcaagacaa aataggaaaa gcttcctctc
    38581 accaaaaggt tcgttcctgt tcgctatctc tttattgatt tgccagaccg gcaacaagtt
    38641 gccttttatc accaaaaagt gacaaggaca agggtaaatg gcacgctgcg cgcccttgtc
    38701 cttgtcgctt ttcgttgatt cattctggca aacaaatcaa taaagaaaga actaacgaaa
    38761 ggaagtaaaa cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa tttgattatt
    38821 ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accacatact gtcgaatgcg
    38881 attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt gtatcaatgt ccaacatgcg
    38941 ggaaaaaata taccttgtct tatggcgatt atattttgat tgatgagaaa ggaaagaaac
    39001 gctgatgaaa acacaacaca aattattgac tgtaaaagaa gtcgttcaaa ttttggtgga
    39061 caaagaagtc atttctgaaa aagtagcaga agctttactt attccgcggt actggacgat
    39121 agaaacccct ttagtagagg atgcaccgac tatgctattt gaagacctag tgaagatagc
    39181 aggatatgaa ctgcattatc aagaaaactt ggaaacttat gaaaacgaat attggttaac
    39241 caaattagag aactaaaaag gagaaaaaaa tatggctcaa aattactatg acgaatttgt
    39301 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tatacaacga
    39361 aacacgtgtt ccaaaaaaac attataaaga aaaattgtcg gtagctatgg aagaaatgat
    39421 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacactgg aagaactgaa
    39481 aaagcaaaat gcaaaatggt ttttccaagc cttactatgc ttggaagtag gtgtcaaacc
    39541 aagcactatt aaaccttctg aatatcaagc tttagaacta acgtatgcga aatttgtcga
    39601 aacgaaaaaa gccaagactg tttcaagtga atggttagat tattttgaaa atatcaataa
    39661 atatggcgcc tattacacca tgaaaaagga ggacaacgaa aatgaataag gtgatttatc
    39721 aactaacaac ggaagatgtg tcacactttt taacaaacaa agaacatttg aatgaagtaa
    39781 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
    39841 tggaagaaca gttgtatttg gaaagtgaaa cgagtcaaac gaaggtcaca gtcagcaata
    39901 attatttaga cgtgtataaa cgtcgccctg acacattaaa aaaaggagcg gagcttctac
    39961 gactagaaac agaaaaatat atcatgaatg tttacctaga aaggagtgaa ctggtcgaaa
    40021 actttgtcag ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa
    40081 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaagatgcga
    40141 cggtagacga ttattacctt gtatttacta gatttttgac gtgtgggacg gaatctatcg
    40201 tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg gaatatgtag
    40261 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagacacct
    40321 acacttacga cgatacataa gaaatccaaa atgaacttga ccgtaaacga atgccatatc
    40381 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag aatgtgtgaa
    40441 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
    40501 ccgcccccac cgcttaggtg gtgcgaaata agcgagcaaa gcccgaaaac ctaagagaaa
    40561 gaaggagatt tatatgaaca aaattattcc cttattatta gtcctatttg ttggagaaat
    40621 acttatgcga cttagtgcag ctgtacattt aaacctcgca gtatttttgg taatactaat
    40681 gttttgtttg atcttttggc gatttgtgtt agcccttgtg agtctaatat tcaagttgat
    40741 tgcagctata tttttcatag ggttatttcg atgaaaggag agagtaccaa tgccaattgc
    40801 aataggaaat aaacggttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagta
    40861 accaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatctatt
    40921 gatcgcgcaa gaaaaagcag gatttgatgt accagcacta aaaaagtgcc gtcacttttt
    40981 aacttgatcc gtataagtga tatcactttt agcaaggagg gaaataaatg ttaaaaacta
    41041 aaaagaattc gtacaaatta aagcttagta tactatctat tatttgtctt gtagatatga
    41101 taattagtgg tcatttgtat agagtggcat atacgcagtt tattgagtac aaggttcact
    41161 atttaggatt agctgggaat cgatacgttg aatatgtcca ttattcatta aaatgttctt
    41221 cgtatattct tctttggctt attttttcat tgtttgaatt gctaaattta atagcaatat
    41281 cttcatatca cttagataag ctaaacacca aatctagccg ttataacgaa gtgttcaagg
    41341 aaaatatgaa gttagaaagt gacttaaaca agctaaaaaa aataaaaaga agaaaacata
    41401 agttaactaa aattatttta ttaagaatga tggaatataa ggataaggct accaacataa
    41461 cgatcattac ttctctggta ggctatggac ttctagcaat cagtattttg tctatagatt
    41521 atggagtaac tatactgcaa gtcttagttt tggctatacc tatgtatcta tcattgttta
    41581 tcatgcatga aatatataag ctatttgttt tatcaaaaaa tgtagaaata cgggtaaata
    41641 ttttggtgaa tagttatcaa aaagaactta aagaaagaag acggtggcgg tatttattga
    41701 aaggatgaaa aagtaggatt tgacatactg gtattcagaa agtgccgtca cttttaaaac
    41761 aatggaaaag gtggagataa aaatgaagaa aaaacaaacc agactatcgt tagcatttaa
    41821 aaaaacagaa ttactagaat aagaagtgcg accaacaggg tataccctgt tggatttttt
    41881 tagggctatg gtagaatcat aattgtcatt tttattttta aatgacaaaa aatttaattt
    41941 ttaattggca caaactataa atctgtatac aagaaaaa
//
LOCUS       DADXTH010000017        40637 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.017, whole genome shotgun sequence.
ACCESSION   DADXTH010000017 DADXTH010000000
VERSION     DADXTH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.017"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(17..331)
                     /gene="trxA"
                     /locus_tag="IWI97_000859"
     CDS             complement(17..331)
                     /gene="trxA"
                     /locus_tag="IWI97_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBC2462381.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(418..2778)
                     /locus_tag="IWI97_000860"
     CDS             complement(418..2778)
                     /locus_tag="IWI97_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBC2462382.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(2893..3441)
                     /locus_tag="IWI97_000861"
     CDS             complement(2893..3441)
                     /locus_tag="IWI97_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBC2462383.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(3499..3936)
                     /gene="zapA"
                     /locus_tag="IWI97_000862"
     CDS             complement(3499..3936)
                     /gene="zapA"
                     /locus_tag="IWI97_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBC2462384.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            4138..5055
                     /locus_tag="IWI97_000863"
     CDS             4138..5055
                     /locus_tag="IWI97_000863"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBC2462385.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(5311..6222)
                     /locus_tag="IWI97_000864"
     CDS             complement(5311..6222)
                     /locus_tag="IWI97_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBC2462386.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(6625..7203)
                     /locus_tag="IWI97_000865"
     CDS             complement(6625..7203)
                     /locus_tag="IWI97_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2462387.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(7223..7891)
                     /locus_tag="IWI97_000866"
     CDS             complement(7223..7891)
                     /locus_tag="IWI97_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462388.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(7906..8976)
                     /locus_tag="IWI97_000867"
     CDS             complement(7906..8976)
                     /locus_tag="IWI97_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462389.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(9147..10511)
                     /locus_tag="IWI97_000868"
     CDS             complement(9147..10511)
                     /locus_tag="IWI97_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2462390.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(10508..12067)
                     /locus_tag="IWI97_000869"
     CDS             complement(10508..12067)
                     /locus_tag="IWI97_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462391.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(12123..14603)
                     /locus_tag="IWI97_000870"
     CDS             complement(12123..14603)
                     /locus_tag="IWI97_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462392.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(14587..14922)
                     /locus_tag="IWI97_000871"
     CDS             complement(14587..14922)
                     /locus_tag="IWI97_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462393.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(14936..15970)
                     /locus_tag="IWI97_000872"
     CDS             complement(14936..15970)
                     /locus_tag="IWI97_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBC2462394.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(16060..16740)
                     /locus_tag="IWI97_000873"
     CDS             complement(16060..16740)
                     /locus_tag="IWI97_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBC2462395.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(17024..18127)
                     /locus_tag="IWI97_000874"
     CDS             complement(17024..18127)
                     /locus_tag="IWI97_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBC2462396.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(18756..19226)
                     /locus_tag="IWI97_000875"
     CDS             complement(18756..19226)
                     /locus_tag="IWI97_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBC2462397.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            19556..21196
                     /locus_tag="IWI97_000876"
     CDS             19556..21196
                     /locus_tag="IWI97_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBC2462398.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            21350..22672
                     /locus_tag="IWI97_000877"
     CDS             21350..22672
                     /locus_tag="IWI97_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBC2462399.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(22740..23174)
                     /locus_tag="IWI97_000878"
     CDS             complement(22740..23174)
                     /locus_tag="IWI97_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2462400.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(23239..24075)
                     /locus_tag="IWI97_000879"
     CDS             complement(23239..24075)
                     /locus_tag="IWI97_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBC2462401.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(24817..25443)
                     /locus_tag="IWI97_000880"
     CDS             complement(24817..25443)
                     /locus_tag="IWI97_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462402.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(25738..26073)
                     /locus_tag="IWI97_000881"
     CDS             complement(25738..26073)
                     /locus_tag="IWI97_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBC2462403.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(26060..26344)
                     /locus_tag="IWI97_000882"
     CDS             complement(26060..26344)
                     /locus_tag="IWI97_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBC2462404.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(26400..27923)
                     /locus_tag="IWI97_000883"
     CDS             complement(26400..27923)
                     /locus_tag="IWI97_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBC2462405.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(27916..29091)
                     /locus_tag="IWI97_000884"
     CDS             complement(27916..29091)
                     /locus_tag="IWI97_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBC2462406.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(29095..29280)
                     /locus_tag="IWI97_000885"
     CDS             complement(29095..29280)
                     /locus_tag="IWI97_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462407.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(29246..30940)
                     /locus_tag="IWI97_000886"
     CDS             complement(29246..30940)
                     /locus_tag="IWI97_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBC2462408.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(30937..31410)
                     /locus_tag="IWI97_000887"
     CDS             complement(30937..31410)
                     /locus_tag="IWI97_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBC2462409.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(31479..31634)
                     /locus_tag="IWI97_000888"
     CDS             complement(31479..31634)
                     /locus_tag="IWI97_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HBC2462410.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(31768..32148)
                     /locus_tag="IWI97_000889"
     CDS             complement(31768..32148)
                     /locus_tag="IWI97_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBC2462411.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(32152..32367)
                     /locus_tag="IWI97_000890"
     CDS             complement(32152..32367)
                     /locus_tag="IWI97_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462412.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(32370..32777)
                     /locus_tag="IWI97_000891"
     CDS             complement(32370..32777)
                     /locus_tag="IWI97_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HBC2462413.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(33043..34518)
                     /locus_tag="IWI97_000892"
     CDS             complement(33043..34518)
                     /locus_tag="IWI97_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBC2462414.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(34508..35356)
                     /locus_tag="IWI97_000893"
     CDS             complement(34508..35356)
                     /locus_tag="IWI97_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HBC2462415.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(35393..35755)
                     /locus_tag="IWI97_000894"
     CDS             complement(35393..35755)
                     /locus_tag="IWI97_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462416.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(35756..35914)
                     /locus_tag="IWI97_000895"
     CDS             complement(35756..35914)
                     /locus_tag="IWI97_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462417.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(36055..36679)
                     /locus_tag="IWI97_000896"
                     /pseudo
     CDS             complement(36055..36679)
                     /locus_tag="IWI97_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(36676..36987)
                     /locus_tag="IWI97_000897"
     CDS             complement(36676..36987)
                     /locus_tag="IWI97_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462418.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(37031..37324)
                     /locus_tag="IWI97_000898"
     CDS             complement(37031..37324)
                     /locus_tag="IWI97_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462419.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            37518..38165
                     /locus_tag="IWI97_000899"
     CDS             37518..38165
                     /locus_tag="IWI97_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2462420.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            38226..39371
                     /locus_tag="IWI97_000900"
     CDS             38226..39371
                     /locus_tag="IWI97_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2462421.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(39452..39757)
                     /locus_tag="IWI97_000901"
     CDS             complement(39452..39757)
                     /locus_tag="IWI97_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBC2462422.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(39782..40213)
                     /gene="ruvX"
                     /locus_tag="IWI97_000902"
     CDS             complement(39782..40213)
                     /gene="ruvX"
                     /locus_tag="IWI97_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBC2462423.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(40210..40476)
                     /locus_tag="IWI97_000903"
     CDS             complement(40210..40476)
                     /locus_tag="IWI97_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBC2462424.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
BASE COUNT        11714 a         8412 c         6854 g        13657 t
ORIGIN      
        1 tttttgctgt aagtttttat aaatattgat cgatcatttg acgtagttgt tcttttgaat
       61 gaacaccaac agctttttct actacttgac catctttttt caacattaat gttggaatgc
      121 tcatgatacc gaattgttga ggagtttctg gattttcatc cacatccatt ttagcaattc
      181 tgaattcttc ttcatcatat tcttgttcca actgatccaa aatcggtgct tgcatacgac
      241 atggaccaca ccaagttgcc caaaaatcaa tcaatacaag tcctttatct gtttcttcat
      301 taaatgtctt atctgtaatt acttgtgaca tatcaaagac ctccattttt aaaatctatg
      361 attagtatag catttctata cttggatagc gaaccatctg cttaccttta gtaagcttta
      421 cttaaattta acaattgtcg caccatttcc gccttggttt gccggtgcaa actcaaagct
      481 tttgacgctc cgatggtttt taagataatc agtaattccc ttgcgaagtg cgcctgttcc
      541 ttttccgtgg acaatagtca cttgaggata accagctaga atggcagaat ccaaatattg
      601 atctacctca cttagcgctt cttcgtatcg ttttcctcga aggtccaatt gattcggcac
      661 gtgactacta gattcagatc gtacagcatg gataactcgt tgtgtcggct ctttttgagg
      721 agcaacaggc gtcagctcgt cttcggacac attcattttt aatataccga tttctacttg
      781 ccactgattc ccgtttttac gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac
      841 ttcgtctccc ggtttcaacg ttttttgttc tttcgccttc ttcaatactt tatttttctt
      901 caaagtttct tcttgatgta gatttgctaa ttgggtcttt gcatcgatca actgatgctc
      961 tttcacattt ccttgcccaa tctgctgttg cattttacga atatcagcaa tgattttttc
     1021 tgctttttct tccgcctctg acacgacttc gtttgctttt ttctttgctt tttccatttc
     1081 tttttcacgt tcttcaaaga aatagctgta agcttctttc aaatcatcgt gaagttcttg
     1141 tgcctcagaa acaaaatgac gcatttccag atattcggtc tctgccattt tccggcgatt
     1201 ctctaaatct gtgatcattt cgtttaaatc ttggctctca tcattcatca actgcttcgc
     1261 ttcatcaata acttctgtat caagacctaa tcggctagag atttcaaacg cattgcttcg
     1321 tccaggaacg ccaatcagca aacgatacgt tggacttagt gtatccacat cgaactccat
     1381 gctggcatta atcgtatttg cccgattgta tccataaact ttcaattcag gataatgcgt
     1441 tgttgccatc acataagcag actttttacc aagatcatct aagattgcga ttgctagagc
     1501 agcaccttct tgcggatctg ttcctgcacc gagctcgtca aataatacca gacttttttc
     1561 attgactttt gataaaatat ctacagtgtt cgtcatatga gaagaaaagg tcgatagact
     1621 ttgctcaatc gactgttcgt ctcctatatc cgcaaatact tcctcaaaaa taccgatttg
     1681 actttcttca cctgcaggaa tcggtaatcc tgcttgcccc atcagttgta acagacctaa
     1741 agtcttcaac gtgatcgttt tcccacctgt gttaggtcct gtaatgacga tggcttgata
     1801 gtcttttccg attgtgatgt cattagaaac tactttttcc tgatcgatca gtggatgtct
     1861 tgcttgtttc aagacgacat gattgtcttc actgattccc ggcacgatgg cttttacttc
     1921 tttaccaaat cgtgcttttg cattcatcag atccatttta ccaatcacat aagcattgtg
     1981 gatgatttct tgacgatggg gaacaagttc agcagaaagt tcacttaaaa tacgttgaat
     2041 ctcatttcgt tcagcgattt ggtattgacg caaacgatta ttcaaatcca ccacttgttt
     2101 tggttcaata aataaagtct gtcctgaagc actttgatcg tggactacac cgccaaatac
     2161 accgcggtat tcttgtttga ctgggataac gtagcggtca tttcgcatgg tgatgatcgc
     2221 atcacttaaa tactttgcat ttttcccacg gacgatccca tctaactgtt cacgaacagc
     2281 ttgttcactt cgccgaatgt tctgtcttat gctctttaac tcaggagaag cgtcatcagt
     2341 cacgcgtccg tcttcatcga ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac
     2401 taactgatct gcccacatat acaaacgtgc aaattcgatt tcactgtcag aaagatcatc
     2461 aatgaaacga ttcaattcgc ttgtcgtaga caatactctg gaaacttgtg caagctccac
     2521 tccattcaag tctgctccga tctcgatccg tttcatatgg ggacggatat tttcaagttt
     2581 aggtataggg atcccgcctc gcagacgttg cactttcaat ccatcctcag tttcttgaag
     2641 ccagtttgtg atcgtttgtt tctcactcac tggtaccaat tctgccagtt cttcttttcc
     2701 ttgtgcagtc actacaaatt gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc
     2761 aaggattcgg ttattcattt tatcacctta tctttcacta tgaaaaaaga gctgtgccgg
     2821 acatacgcta cgcagccttc ttaaataaac tactctgtac aaaaaacgaa atattttcca
     2881 gcactatttt ccttaattca aaatattcgt tacccaaagc tcatacatct tgttggtaag
     2941 aaatggcgtt ctctcaacga taaaacgagc gaaactatgc tcgccgaatt gattttggac
     3001 cacatctaat ggaacaaagg caagtaactg caaaatcaaa aatatagtaa cgtaagccat
     3061 gattagtgac aggatgcctc ctgcaatcca gtctacctgt ctcaagatcg gcacatacgt
     3121 caatccatgg acaaaaaccc ccacaaatcg ggtaagcagc cagccaatta gcaaaataat
     3181 cagaaaagca gttcctgcat aaaaagcttc atctaggtga aaagcaaata cctgatcaaa
     3241 aaatgctaac tttgatgttg gggtgacagc tgggtatgga atgtataatt ccaaatgact
     3301 tgctaatggt ttgtaaaaat gctgggcagc aataaatgac aatacatatc caatcgcgaa
     3361 tatgatttgc aaagcaaacc cgcgacgtgc tccagaaaaa aacgcaatta gcaataaaaa
     3421 taaaatcagc aaactcagca tgctacgttc ctttctcttc taactttttt aaatttttct
     3481 tattttgata aaaggatctt aattagaaga tttttgttta atttgtcctt ttcgtttctc
     3541 gtttaaaatc tgttgtgctt ccacatgatt tttaatggta tgatctgctt gaccattttc
     3601 tttcaatact tcccgagctt cgttctcgat tgcttcaatg cgtttcacac gattttcaag
     3661 ctctgtaaat ttgatcattt ttttctttaa ttctgctgtt tcttcttcca gctccaagat
     3721 tctttcttgt ttcttcaatt gatccgaaag cgcattgaca gccatcaaga ttgctgcttg
     3781 ttcattatcc atttgtggtg ataattgttt caattctgct aactgttcat tgatcagctt
     3841 cgttacgata tccatatgat gcttggtttc ccgtccgata atagtatatg tttggttggc
     3901 aatcactgct ttgtagcgtg ttttttcgtg cgccatacga atcatttcct cctatttgat
     3961 gaaagtttta tttagtgtac catgaagttg gttctttcac cgttctccac aaatattttt
     4021 cacatactta actattatat gataaacttt aaaaaaatgc tatgtctcgc aatctcttag
     4081 aaaacgaaaa gtcgtgagtt gcggtatagt tttatgtata aaaacgagga gaattttatg
     4141 agcaacgtca ttatcaaagc aacaaacgac caacttagtc aaatgaaagc atactatcgc
     4201 tcttatttgt taaataaaca gattccttat actagctttg ctgcaaaaaa agacgggacg
     4261 accattaccg cttatacatc cggaaaagtg atgtttcaag gtacgaatgc agcaatagaa
     4321 gctgataaat ggggggaggc acaggataaa caagccaagc cagcaaaaac agcctcttct
     4381 ttgccaaaaa atatcagtca attatccgtt ttaggtagtg atgaggtcgg aaatggcagc
     4441 tattttggtc cgctaacagt atgtgctgct tatgtgaaac gtgatcagct tgcagaatta
     4501 cgtaaacttg gtgttagaga ttcaaaagag ttaaaagata gccaaatcat ccagcttgct
     4561 gacatcttaa aaaaaaccat cccctataaa ctgcttgtct tatctccaga aaaatacaat
     4621 gagatccaac caaaatacaa tgccgttcgg atgaaagtcg ctttgcacaa tcaagcgatc
     4681 catttgctgc taaaaaaaat cgagccagaa aaaccagaag cgattttgat cgatcagttt
     4741 acgccggaag caaattttaa aaaatatgcg cgtttagaaa aaaatcaatt acaagaaaaa
     4801 ctgtacttcg ttactaaagg agaacaatat catttagctg ttgctgctgc ttctatcatc
     4861 agtcgtgcca gctttttgga agagttacac aaagcatcag ctgaagctgg aatcaccctt
     4921 ccatcaggag ctggaacgaa atctgaccaa gtagcggcaa aactattgga gaaaggcggc
     4981 atgcctatgc ttgaaaaata tgccaaactt cattttgcca atacagaaaa agctttgaag
     5041 ctgctaaaaa aataatggaa ctgtgacaaa agtcatgtca cagtcccttg ttccgaataa
     5101 acggtgttct catgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc
     5161 tgttttttga aattgctcct tgtgacgcgg agttaaacgg caggctcaca acctcttcct
     5221 taaacggtgt tcaaaagctt gcatctgcga taataagccc ggccaaacga aaaatatgag
     5281 gagctatttt tgtttggctg ggcttattta ttactgaaaa taagtggtca atgcttgtgt
     5341 caaacttgca gcaatctgct gctgataact gtctgttact aattccttta aatcactatc
     5401 attattcata taacccatct cgattaaaat agaaggttga agattttctc gaagaacttg
     5461 atagttccca gtggagattc ctcttgaatc cagaggcaga tcagtgaagg agtcagcaat
     5521 tgtctgtgct aatgtttcct cttgtccata gtaataatat gtcgtgattc cagttgcttc
     5581 attcgcatat tccgtagagt ctgcgtgtaa actgataaag acatctgcct ttttttcttc
     5641 actgtagtcg catatatctc caagctgtac taattcatct gtagtatggg tcaaataaac
     5701 cgttgctcca gcatcctcta gcgccttttt tacttttatg gctgtactta atgttatatc
     5761 tttttcttct gtttgttcat cattgcttaa tgccccagta tcatttccgc catgcccagg
     5821 atctaataca atcactgcat cagatagctc ttccggtttt gtttgtatat tctttgtgac
     5881 tttggttcca tcttcgaggg ctaaatatcc agtatagcca tcatttgtct tgatctgata
     5941 agcatttttt gtttcagaaa gaaactccgc cagctctccg ccgtttatag ttgcaagtgg
     6001 ggtagttagc gagtcatctg tatatagtgt acggtcattt tttccgattt tgattaatgt
     6061 ctcttgttct gtatcatcaa atgattcctt tgtctttgta accgtactgt tcgtactgct
     6121 gaccgtttgt ccaactatct ttttttgacc aaaaacatct atttgaaaac agataataat
     6181 gataatgatt gcaaatacaa aagctccaat tatttttttc attcttgact ctcacctcgt
     6241 agatccagac taagggtcaa aacttaaaat cagcttaagt acgcaaaaaa ggggctgtaa
     6301 aagatataac cgtagaaaat aacattactg ctaaaatgat ttttttcatt tttggcatat
     6361 cctgaaatct ccttttgact attcgtggtg attcatctac ccaataaata gtttccttca
     6421 ttctacaaac aattaatttc ttcgttatta aattaaaaca cttcctggaa caccgacata
     6481 ataaataaaa atataccctg ataatcatga gagaataaca aatttgtatt tatgaaaaac
     6541 tacctatata aaaaaaacaa gatataacga tcttcgtcat atcttgcttc ttcacctaac
     6601 tatttttctc cctatataac agctttacgc ttctcctaat acaccattca aatataaatc
     6661 gatcaattgc gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg
     6721 tgctaacact aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac
     6781 agattggttt gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa
     6841 gaaaagatga tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa
     6901 caaatcgacc agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa
     6961 tcccgtttcg cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt
     7021 ttctaaaact gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggttg
     7081 ggtgatccca actttttgtg cgatctctct tgttgaagtg ccgagatatc ccttggacat
     7141 aaacaagtca gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac
     7201 catttttcca cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa
     7261 atattctatc gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat
     7321 ttttgtcttt tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca
     7381 aactagcagt tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga
     7441 ttgcgaccct ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat
     7501 ccacgccaag ctgctcaaat aattcttttg cttctttttg acgatgttca tgtttgatct
     7561 tatctactaa taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca
     7621 aaataaagcc aatttcttta aaacgtagtt taacgcgttc actttctttt tgattactga
     7681 attcttgatc attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag
     7741 tcaaaaaagt acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat
     7801 cgatactcat ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt
     7861 tttctacttc tttaaattct attgctttca tgctcttcct ccttcttatc caattgcctt
     7921 caacggatcg atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac
     7981 cagcatcaac aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc
     8041 aggtagaaca agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga
     8101 taaaatgaat gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc
     8161 tttcattaca ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat
     8221 cacaatagcc gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc
     8281 attatatcca ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg
     8341 taaattactg attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc
     8401 ttctttttcg aagcggatct cttggtaagc attgatggtt gtatagagaa ctggtgagac
     8461 attgaatttg gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa
     8521 cttaactgta tctcctacgg ctagaccata ttcttctttc aaactaatat cgcctactgc
     8581 ctcatcatcg ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt
     8641 atcgataccg aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac
     8701 gactccaggt gtctgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt
     8761 cttagtaatc atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc
     8821 tgcctcccac ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt
     8881 caaaaaaaat accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa
     8941 ctttgaatgc ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc
     9001 attttataca acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt
     9061 cttccgctat ttcattatat gaatttttta ttctcgattg attaatattt attatatccc
     9121 cacacaataa aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct
     9181 ctcttagcta gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtgttca
     9241 gacagaaatc caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata
     9301 tccgcaagca ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt
     9361 atttctaatg tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcatcg
     9421 tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc
     9481 gcttcatctt ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt
     9541 atcttttgtc tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt
     9601 gccatatgta cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa
     9661 ctgactggga cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca
     9721 ttgactatca cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta
     9781 ggacgagttt gggcagaaaa aatgaccata tctgccttta ttgcttgaga tagaagttgt
     9841 acggatacta ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa
     9901 ttgatttgct tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat
     9961 cttgttaaaa cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa
    10021 gcatctgcta attccaatcc aatataacca gcaccgaaga tcactacttc ttttgcttct
    10081 tttaaagcgg caagtacttg cttagattgc actagatttt tataggtgta gacattttca
    10141 gcttcaattt gtttatacaa ggtgacagga gaggaacctg ttgctacgat taatttatca
    10201 taggttatgg tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga
    10261 tcaagttcag ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc
    10321 atgttatggg caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca
    10381 tttgaaacat agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc
    10441 ggttgaagtt ttcttagatt tagggcagct gtgatgcctg catgggaagc accaatgatc
    10501 acgattttca tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa
    10561 attggttcaa ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag
    10621 cggacttctg tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt
    10681 ctttctcgta ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt
    10741 ctttcacgat atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta
    10801 attctaactg cttgatcagc gtccgcaaaa tagatgcata gcgagcaaat aattcaaagc
    10861 gggcattttc ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc
    10921 tttcacataa aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt
    10981 cttggccaaa aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca
    11041 ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt
    11101 ctatcgattg tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc
    11161 ttatcaaaaa ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga
    11221 aaagataaaa aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac
    11281 gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa
    11341 gattgctata atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa
    11401 gccatatttc aaatttcaat cgtccttctt ctgtaatagt aaaactaaac tctttttgca
    11461 gcagatctaa aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagataag
    11521 gacgtccaaa ccaaaataat tggaaataaa aataacaaat ttgactttct ggaccggtca
    11581 gccgcccgct ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc
    11641 tgcgagacaa agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg
    11701 agccattttc caacagataa agaagtatct gatattttgt agaatagcgc aagtagcaat
    11761 gataataaac atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt
    11821 ggataacaat ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt
    11881 ctttcaaggt agcccgatca actgataagc gggtaagtac gttattaacg gaaatctctc
    11941 ctttttcctc taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca
    12001 aactgaaaag ttccaagtcc actcctccaa gcatttctga ttttaacagc agaagtttcc
    12061 ttctcatcct tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc
    12121 acttagctgg ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca
    12181 ggttgagcat tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca
    12241 acgagaacct ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc
    12301 tgaatcaatt gttttgatcc agaatgattg aagtataatt ttgttccgct taattcttga
    12361 ccttgatcat tcttaaataa agaagtcaat gtccctgtca gttgccaagg agacgtgctt
    12421 ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt ttgcttcata ataacttgtc
    12481 ttcaacggtt tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt
    12541 aattcttgtt tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc
    12601 ttagcaccat ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg
    12661 acggattgta cagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta
    12721 tctcctattt tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct
    12781 gcgatagttg taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt
    12841 gagatcgtga tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca
    12901 ttttcttgaa tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg
    12961 ctaaggtttt ttagctgact atctgcactc acagatggaa aattagccac tgttatttga
    13021 ggaagagtgg tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg
    13081 ttagtaagta catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca
    13141 ggtagtgtag aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt
    13201 tgatctgtcc atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc
    13261 gaaccctttt ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa
    13321 aggagctttc cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc
    13381 ttgtactgct ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc
    13441 ccactgtaca cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc
    13501 agtattgctc cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga
    13561 ctaaatggca actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg
    13621 gtagagcctg gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta
    13681 ttatattttc ctgttgaatc acttgttgag atgtaactat tccacgtatt tatctctgca
    13741 cttgcagaaa atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat
    13801 ctacctgcag ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa
    13861 tcataaaaat caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg
    13921 gtcaaaattt tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca
    13981 ttagctgtaa catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata
    14041 atagcaataa tatctgccaa tagagtattt gtatcccacg accccatccc aaaacgcaag
    14101 gtatggcttc ctgctgataa taaactaccg ctagtaacct tgatgggggc accggcaata
    14161 tacaagtagg cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc
    14221 ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt
    14281 acatttgtta gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga
    14341 tcatttgaac catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt
    14401 agttggacaa gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt
    14461 tcatacgtgt atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa
    14521 ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat
    14581 gaaaatttat tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa
    14641 ccacgcaaga agccataatt ctataccaat gatcaaataa agaaaacgga tcttttcttc
    14701 attcgtctcc gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg
    14761 ctgttttgat gcgccattga ctgaaccact tggatgcgga actggactta gatcataaaa
    14821 tgtaatcgta cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag
    14881 caaacttgca ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg
    14941 ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca
    15001 ctagaactgc agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa
    15061 aaacggaatt ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa
    15121 atcgccattc attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt
    15181 ttgctttatc accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa
    15241 attcattaga cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata
    15301 tcaaattgct gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg
    15361 cgttgacctc gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac
    15421 tttcttgtaa aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg
    15481 tagcattttc tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc
    15541 ccttaaagct ttttgctggg attttcactt ctataggtat agttgtttct gcatgtgctg
    15601 atacattgat ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct
    15661 catctaacgt tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg
    15721 gattgacaga gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta
    15781 gtgtctgatt ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt
    15841 ggttctctgg aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga
    15901 ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac
    15961 tttgattcat tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta
    16021 tagtattttc accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac
    16081 gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt
    16141 gatatttgct ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt
    16201 aaatattggt gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc
    16261 agtggattcg ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc
    16321 ttgcaaaact tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg
    16381 tgcttggcca tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt
    16441 ttttgaagcg tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg
    16501 caattcagga actacatcca atgttaaaga acctgtctgg ccagttgctg gattttccgg
    16561 atctgctggc tctactggtt ttgaaggatc agtagggtta agaggctcaa cggtatttgt
    16621 tggttcagta aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt
    16681 tgccaagccg cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat
    16741 aataaatcac tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc
    16801 tcatgaattc atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca
    16861 ataaaatcgt ttacatgaat attcttcctt ttttattaag tatgaaaaaa tatttcaatt
    16921 ttattttttc gtttcaaatt attctattag tgacatcctt gaactataaa aataggagta
    16981 cggcatcatc tgccgcactc ctattttttt cgtcatttat ttcttaaata aaacgattct
    17041 cacgcaattt tatttttttg atatcttcta ctgtttgtgt tccagctaat tgcatgacca
    17101 tttccaattc tgttttaaag aaatcaaaca cttgatggac tcctgtactt cctcctaaag
    17161 acaatccata aataacgggt ctgccaatag caacaagatc ggcacctgat gcgattgctt
    17221 tgaaaacatg ttgtccacgt cgtacaccgc tgtcaaacac gattggtaca cgtttatcta
    17281 ctgcttctgc cacatattgc aacgaatcaa acgcagcagg ccccccatct aattgacggc
    17341 caccatggtt agatacccaa attccaccag caccagattc taatgatcga tagacatctt
    17401 cttctgattg aacacctttc acgtagactg gcaaatcaga atgagcagcg ataaattcta
    17461 catcttttgg actaagtttt tgtttggaag atttatatac agcatccatc gtttggccta
    17521 ctcctgattg ataagcctga acgatcggca ttggcagcgg gaaggtaaac ccgtttctgc
    17581 ggtctgtttc acggtttccg ccaactgttg catccgctgt caatacgatt gcttttgctc
    17641 cattacgctt ggccacttca agaatatcca aattgattcc attatctttg ctcatataaa
    17701 attgaaacca ttgcggtgct ttctccccgc ctgcttctct tatctcttct aaagtacaag
    17761 aagcgtaaga acttgctgta tagatcgtcc caaaatctgc tacacctttt gcagaagctt
    17821 tctccgcctt cacatgagct aatccatgag cagcaactgg tgccatgatg attggtgcag
    17881 tcagcatttc ttcgtcaaaa tgtgtagttg tatcgggtaa ttctacatct cttaatacat
    17941 gaggaataat caaacgatga ttgaatgccc gttcattttc ttgataagta aataagtcgc
    18001 cagcaccact gctgatgtaa ccataaccac cagctggaat cacttgtgaa gccgcgattt
    18061 ctaaatcttc catattgata aaatctacta ttccttcatt tgtacctgct tgatatgttt
    18121 tttccatctc aatgccttct ctctataaaa tttgaatgat gaacaatatg atagcgttta
    18181 cactttttat attactcttc gacattgata caggcaaata atctgtttac atttcagcca
    18241 ttccagatgt cactactctg gaagaaatga ttgtaataaa aatagataaa aaaccgatgg
    18301 gactttgaca aaagtagtgt caaagtccct ttttttgaat aaacggtgtt caaaagccat
    18361 tttctcggta ttaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt
    18421 cttactactc gaaactaagc gactttttca caacctctcg ataaaacggt gttcaatcag
    18481 ctgaccctcg gtattagctc aaaaaatggc aaaatatgag gaactatttt gccatttgtc
    18541 tctctaatac tcaggtcaga acgttgactt cacagtctct tccttaaacg gtgttcaaaa
    18601 acttgcatct gcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg
    18661 gctgttctta ttacttgatg cagggcaggt ttttcacgac ctctgattca cggtgtttct
    18721 ttttcatcaa catttttacg aaatactgtg gaagactatt gaaataagtg tggattgttt
    18781 ctttgtgttt caatcgtttc ttgccattct tcatttaatt ttggattacc gttttcttcc
    18841 ttgaactcat aaggtcctaa ttcaaaaatc ccagacagta tttgattcac gatttcatat
    18901 agttcagtgt gatcagacac atgattcttg aagatcatct cattatcacc acgtttgaac
    18961 ctcacattcg ggctctcatg atgaacctct atgcattttt ctcctttttt taactgacgg
    19021 acttctgaaa acatctcata aatatcttct ccagtaaaac cttcagcaag cgccagactg
    19081 tccacaagac cgcctactgt cagttcagag attattgctt gattggaatc taattttaat
    19141 gtatagcttg tcgcttctac tacgccacat gcattgcaac cgccttctga tagttcgctt
    19201 tttctaatca attgaacttc tttcattttc tcttcctcct cttaattttc tacttatgta
    19261 tttttatcac ctgacatagg tgactcctat caacgctatc atgtgctttt ttcctcagaa
    19321 atcctattta tccattccat ctatccctta ttcttctact tttctttctc tagcatataa
    19381 gagatacgca tagtattcaa gcaaggattg tcgatataaa aagaacaaat actgctattt
    19441 tgatttcttt tggctatatt acatataaat aaaatttatc atacctgctc tttttgacta
    19501 tatcatcatc tgacttctat acttcgtttg tattaaaact taggaggaat cattcatgct
    19561 tacagggtta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
    19621 agaacgggca aaatatggaa tcactggtat gcttccaagt actgtccaaa cactggaaca
    19681 acaatctgtt caagcttatg gtcaatactt aagcaaacaa acagatttgg aaaaacgtat
    19741 cttcttaatg aatctattca atacgaatcg gacattgttt tataaattga tgggcgaaca
    19801 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
    19861 caacgaaatt ttcttgaaac cgcaagatgc tgcatttctt tcaatcgacg aaccagaaaa
    19921 catcaaagca agtttgaaga acgcggcaga tggacgagac attcgtttga ttgttgtgac
    19981 tgatgcagaa ggaatcttag gtatgggcga ctggggtgtg aacggtgttg acatcgctat
    20041 cggcaaattg atggtctata ctgctgctgc tggtattaat ccagcacaag ttttgcctgt
    20101 atcgatcgac gctggtacta ataatgaaga attattgaat aatcccttat acttaggcaa
    20161 tcgtcatgct cgtgtagaag gcgaaactta ctatgaattc atcgatcaat tcgttcaatc
    20221 agctaccgaa ttattcccag aattgttatt gcattgggaa gattttggac gaggaaatgc
    20281 tgcagcgatc ttagaaaaat acgaagatca aatcactact ttcaacgacg atatccaagg
    20341 tacaggaatt gttgtcctag caggtgtatt aggcggattg aatatatccg gtgaacaatt
    20401 gaaagatcaa ataattttga catttggtgc tggtactgct ggtgtgggta tcgcaaacat
    20461 ccttttagat gagatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
    20521 tcaagtagat aaacaaggtt tattgtttga agatacagaa ggattgactc ctggtcaaat
    20581 cccattcgct cgcaaacgct cagatttcac taatgctgat gaattgacaa acttagaagc
    20641 agttgtaaaa gcgatccatc cgacaatcat gattggtact tctacccaac ctggagcatt
    20701 cactgaagaa atcgtgaaag aaatggctgc tcatacacca cgtccgatca tcatgccttt
    20761 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttaatagaat ggacggacgg
    20821 aaaagcatta gttggaacag gtatccctgc tgctgatgtt gaatataatg gtgtaactta
    20881 ccaaatcggt caagcaaata atgctttgat gtacccaggt ttaggacttg gtttgattgc
    20941 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
    21001 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
    21061 attttctcaa acgattgctg aaactgtcgc aaaatcagtt gtcgcacaaa acttgaatcg
    21121 tgaagaaatc acggatatca aagaagcagt tgaatctgct aaatgggttc ctgaatataa
    21181 atctttagaa gattaagatc gttatagtaa aagaacatac agaaactttg tatgacgtct
    21241 aaaaagaaaa aagcgactgc ggcaaaacag cggccagcca actcctgctc ttttgtcgct
    21301 tttctttggc ttttttcata tgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
    21361 aacaacaact gcaactgcag ttgaggaaaa gaaaaaagaa ggtttttggt ccacaaaaat
    21421 caccggcgta agtttgccac tttatttgat catggtcctt gtcctgatca ttgcaatggc
    21481 tttagggaaa ttaccgacga atatgattgg accaatcgca gttttggtta ttttcggtaa
    21541 tttatttcat tttatcggga caaaaatccc tatcgtcaaa agttatttgg gtggtggttc
    21601 cgtttttgcg atcttcgctt ctgctgcgat tgctacattt gggatcttac ctgaatatgt
    21661 agtgaaatcc acagaaaact ttgtcagcaa tatgggcttt tgggactttt atattgctgc
    21721 cttgattact ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg
    21781 ttttattccc gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtggg
    21841 catgcttatc ggaaatggtt ttgcaaattc cgttttatac atctcattac ctgcgatggc
    21901 tggtggtgtt ggagccggag ccgttccttt atcaacgatt tatgcaaatg ttttaggtac
    21961 ggatgcttct tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcttagc
    22021 tatcatcggt gcagcattag ttgctcgtgc tggacaatca atgccaaaat tcaacggcaa
    22081 tggtaaatta atggaaaaag gcgatcttgg cgatggaaaa cctcgaactg tgaaaccttc
    22141 cattccacag ttaggtgttg gtttgatggt atcattgact ttcttcatct taggacaaat
    22201 tggtaactat tttgttccta aagtccatgc ttacgcattt atgattatta tcgttgtcat
    22261 ctgtaaaatc gccaacctat taccagagta ttatgaagat gcagctatca tgttcaataa
    22321 cttgattgtc aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttgctgaa
    22381 tctgaatgta ttagcttctg ctttgacatg gcaatttgtt gtcttatgtc tgacaagtgt
    22441 tatcgcgatt tcagttgttt caggatttgt cggtcgtttg tttggtttat atccaattga
    22501 atcaactatt actgctggct tatgcaacaa ctcgatgggc ggaacaggaa atgtagccgt
    22561 cttatctgca gctaatcgga tggaacttat tgcttttgct caaatgggca atcgacttgg
    22621 tggtgccatc gttttgatca tctctggttt cttgatgcaa ttactttctt agtcaaaaaa
    22681 acaaaaatac ctgatcatca agtatcggct tggtgatcgg gtattttttt gataaagatt
    22741 tacaggattt tcaaaaatct tttttgcatt ttatcggaat gataaccgat gtgttcataa
    22801 aattggtgtg cttctactct gtattctgca gaattaagcc ttattgtcgt tattcctcgt
    22861 tttttcgctt cttcttctac gccagtcatc aaagcctttc cgatgcctct tttttcagca
    22921 tatttagaca cagcaagtgc cataatattg aacattggat cgctaaaaat cgattcatag
    22981 gactccgctt gtacatagcc agctgctttt ttagaagtag gatcttcata aaccaagaaa
    23041 aaatttttct ctggctcctt cagcagtttc ttcatttgtc tcaccgtaac ggttaatggt
    23101 atatcatacc ctaattgatc cgcattgatt tcacgtagtg cttctgcatc ttctaaacgc
    23161 atttcccgta acatccaaat caccagccaa tttctgattt tttaaaatga atttttttat
    23221 catagtatac tctttttttc atttagaaca atatgtttca gtgattttga cttgcgttag
    23281 tctagattct cctaatgttt gtaagatgac aaaggcattg taacaattga ccaaaagaac
    23341 cacgaggaca attacttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa
    23401 tcttgctccc cacaaacctc caataacagc agcggggata acataaaaca acatagataa
    23461 atcaaagcgc ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa
    23521 gaaaatcgta gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat
    23581 caacaaagaa acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag
    23641 aaccccccca catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg
    23701 cttggtgtag aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg
    23761 cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac
    23821 tcctccaaca acagctccac tagataccca accgacaatc tgccaattca gtgatttccc
    23881 gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta
    23941 aaaagaaatc cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac
    24001 gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata
    24061 aatcagacca atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact
    24121 cttttctttt attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt
    24181 aaggaaattt agtgctaaac gaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata
    24241 agaacagcca aacaaaaata gctcctcata attttcgttt ggttgaactt attaccgcag
    24301 atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag cctgccgttc agctccgagt
    24361 cacaaggaac aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc
    24421 gaggagttgg caggagaaca ccgtttaagg aagagactgt gaagtcaacg ttttgacctg
    24481 attattagag aaacaaatgg caaaatagtt cttcatattt cgccgttttt cgagcgaatg
    24541 ccgaggggca gctgattgaa catcgtttat ttggaaaaag ggattgtgac aagacgtttg
    24601 tcacaatccc tttttttata tcttattaat tcattctttt gttctattct tcttcttccg
    24661 ccatgaatga gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact
    24721 aaatgaatta gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat
    24781 atggcataca aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa
    24841 atcaatgagt ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga
    24901 cgaatgtttt ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac
    24961 ttcaccttct agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa
    25021 agcatacaac acattttcca aatttgcttc cttataattt gaagaacgac catgttttac
    25081 tctgttatat attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt
    25141 tagtgaagtc aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc
    25201 gctagattta ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc
    25261 gcaaagttct ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata
    25321 ttcaatagag aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat
    25381 aaactcattt tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc
    25441 cattaaatca cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt
    25501 tgatttctac acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa
    25561 aattgaacgt tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca
    25621 attttcagtc caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc
    25681 cttcatttac taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta
    25741 agaaatctgt ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga
    25801 aataatttta taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc
    25861 ttgatcgtat cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag
    25921 cacgtctttt agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact
    25981 atccaccatc tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc
    26041 agtcaattca tttacattct taatgattgg cataataagc acctctcatt tgttgtacta
    26101 atgcttgtac cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat
    26161 gttgagtaag taatgacacc gccaaatcaa attgctgata attatccata tctttatccg
    26221 ttgcgctact atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa
    26281 taaagtcatc atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta
    26341 ccattttatc tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc
    26401 tatacttttg gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt
    26461 ttcttataat cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc
    26521 cattgagtcg ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata
    26581 atcataggga atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact
    26641 ggcataccga ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt
    26701 ccatctgaat ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt
    26761 ttatttaatg ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca
    26821 gccgttttct tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct
    26881 accaatttcg ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca
    26941 acctcatttg ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta
    27001 aacatttcag cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct
    27061 tgttggttag ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca
    27121 tattgagcta agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg
    27181 ggaacaactg ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg
    27241 atatattctt catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg
    27301 ttattactct ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct
    27361 tggttgttac tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca
    27421 taagcgggaa cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt
    27481 tcaattgtgc catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac
    27541 atagaatcaa caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg
    27601 gttagctctg cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct
    27661 ttgacactag ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa
    27721 tccacctctt ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac
    27781 ggttgcccga atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca
    27841 ttttctttct ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt
    27901 tccttttctt cttcattatt caactggtgt aactccttcc tgttctttta attgatattt
    27961 ctccaacgta tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat
    28021 ggggggtagc cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg
    28081 atccaataca ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa
    28141 gcggatattg gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat
    28201 cagcgtactt tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac
    28261 gcctaaacgc tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt
    28321 ggaattgata agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc
    28381 atcaagaatg atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa
    28441 cttttcacga attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat
    28501 acctgaccca ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg
    28561 aattttcagt tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt
    28621 gaaatatttg aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg
    28681 ataagacaac gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg
    28741 aatttcttct accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa
    28801 catattgacc gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc
    28861 gtttagtaac ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat
    28921 cggacttgaa gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct
    28981 taatgcacga acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg
    29041 ttgtaggaaa ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc
    29101 tcccttctgt tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa
    29161 caagccatag tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag
    29221 aagtaaaaga acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat
    29281 tgtctgcttt cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga
    29341 ttaatgcagc tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt
    29401 tgttatccgt ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt
    29461 gaataacgtt gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa
    29521 tcgttccttg tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg
    29581 aattagcgtt atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca
    29641 aatcttgaat aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa
    29701 tggaacactc accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt
    29761 cttgaagccc atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat
    29821 ctagcggtac gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga
    29881 tataaacggg cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag
    29941 caatcatata gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag
    30001 aattaaggtt attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta
    30061 cttttttgat ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt
    30121 gaatttcttc gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt
    30181 cgccttttag cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa
    30241 gcccagcggt actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa
    30301 tgacgttgta cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg
    30361 gtgcaaaccc atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg
    30421 gcaagtgctg aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact
    30481 tgaccacgtt tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag
    30541 cgttacttac aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat
    30601 tggcagccat gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg
    30661 ctcgattgaa tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa
    30721 tgaatttttg aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa
    30781 tgaatttaat cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact
    30841 tagaacgctc caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa
    30901 tcagcttacc tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa
    30961 cgggtcttcg ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa
    31021 ggtcatacct agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg
    31081 aaccgctgga ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct
    31141 tatctcggct tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc
    31201 ataatctgct tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc
    31261 acttctagcg ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt
    31321 gaacaattct tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt
    31381 tgtatcgtgt aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg
    31441 aacgtaagca attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat
    31501 ttatctatat aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt
    31561 aactcaatgg tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa
    31621 tctcctactt tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa
    31681 ttacccttat gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca
    31741 gtaagtttca cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc
    31801 attggctgtc ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa
    31861 ccgtctattc caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat
    31921 caagccttct tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc
    31981 ctttcgccat gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc
    32041 ttcacgctta attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc
    32101 aatcaatgtt ttacatccag catgattaca ttgtttttta actgccatag attaccaacc
    32161 aaagcgatct cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg
    32221 cgaaactatc ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc
    32281 aaaactaata aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc
    32341 gaagtatcta atactttcca tatgcataat tattctacct ccaataaatt agttatcaat
    32401 tcatcatctg ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc
    32461 tcattcgatg taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat
    32521 tcatccatta gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact
    32581 agcgattgac accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt
    32641 aatacttctg gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc
    32701 gtgttcatta gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt
    32761 gaaataatat tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta
    32821 acaggtaccc gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag
    32881 gtaacgggta aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt
    32941 ttaaggaaat aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt
    33001 aaactagtaa cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc
    33061 agctcgattt ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct
    33121 ttttggcagt tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt
    33181 agtttctgca tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc
    33241 caaaatatca gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc
    33301 attatagaaa tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata
    33361 agaacgtctc gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata
    33421 tttagtgatt gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc
    33481 aatttctaag acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa
    33541 aagcaccttt gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt
    33601 catctttggc ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt
    33661 caaatactgc ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac
    33721 attccgacca taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg
    33781 ttcaatttta gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg
    33841 aagctgcata taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc
    33901 atccgtgtaa agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac
    33961 tggaacgatc tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca
    34021 acgttttgtt acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag
    34081 tgtttccagt ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt
    34141 gaaaacattc tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac
    34201 tacattgtag ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc
    34261 tccagcggta cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc
    34321 gttataagca attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac
    34381 aacactgtta cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt
    34441 caattcaatg atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa
    34501 tttatgctta tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa
    34561 tttgattcac ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca
    34621 attctgctaa agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta
    34681 aatgatcgtt tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg
    34741 tccctgtttt tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta
    34801 ggttcactag ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt
    34861 catatgctct cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag
    34921 tgataatatc aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa
    34981 atgcacctgt ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc
    35041 ctttacctgg tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt
    35101 ttttataatt ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca
    35161 tttgatgatc ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat
    35221 taatttgatt agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt
    35281 tagttccagg agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt
    35341 gataattatt tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag
    35401 acacctccat gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag
    35461 caccttttaa ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt
    35521 gcatatcagt aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc
    35581 gacaaattat tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat
    35641 tgaatgaatt tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat
    35701 taatttcctc taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact
    35761 tatgccctcc agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg
    35821 ctaatcctgc aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga
    35881 ttaaaatgct tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat
    35941 ggtataatcg gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc
    36001 gccaagactg ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt
    36061 tgtctgcaat tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata
    36121 tttttcttta acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa
    36181 catttcagga gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac
    36241 agctttattg gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc
    36301 gtggcttaat ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg
    36361 aaagccttca agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg
    36421 aaatgcctcg gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt
    36481 tgatgatgtt tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc
    36541 catctttagg caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa
    36601 catctttcgc cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt
    36661 tccaaatttc agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa
    36721 ctcaacttgc tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc
    36781 tgcttgtact aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag
    36841 caatttttga tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc
    36901 ttccaagttt gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc
    36961 gattgcttta ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca
    37021 cggcttgcgt ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc
    37081 ttacgactcc atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag
    37141 tattgtatga aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact
    37201 ggtacttttt ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa
    37261 ctcgatcgtc aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt
    37321 ccatatttat cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag
    37381 aggctcttga atacatttct atacctctta taaactcaat ataccatcat accttttcat
    37441 ttgcaagaat tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga
    37501 tatagtagag aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag
    37561 cagtggagac aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac
    37621 aacttggagg taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg
    37681 gctataatct tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca
    37741 ctacctatct attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta
    37801 agcgaaatgt gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg
    37861 attctaatct ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga
    37921 agatttattt aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg
    37981 acgggagtac cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact
    38041 cattaattaa tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg
    38101 aaaatgatct tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa
    38161 aataataata aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa
    38221 ctattatggc aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag
    38281 cctatcttgg aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca
    38341 aaacaaaaaa agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg
    38401 gtattaaaaa agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat
    38461 ataaaaccac tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga
    38521 ttcttccaaa atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa
    38581 ttgtaaatag ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc
    38641 gtatttttag ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat
    38701 taatgcctaa aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac
    38761 aactgatcac cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg
    38821 ataaaacgtt actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat
    38881 tgaattggtc agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc
    38941 aatccaaaaa tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc
    39001 cactagatga aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata
    39061 tgctgacact tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa
    39121 tcacacacca cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat
    39181 ttctcggtgt gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta
    39241 ctatggtaga agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt
    39301 acggtcactt ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa
    39361 atattgacta gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct
    39421 atcatatcaa ggtttctcct agctttatag tctattcttc ttcttccgcc atgaatgtat
    39481 tgaatacttc ttcaatcatg tcccattctg cgtctgtttc gatttgttct aactcgcctt
    39541 ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg
    39601 aaacaccagc aggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg
    39661 ttagtaggat ttcatataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt
    39721 gtccttcatg gtcgtgggaa tggttatgtt ctgtcataag attacctctt ttcttttatt
    39781 attaaaataa ttgcttacct gagccgtcta aataattttg taagatcatc acagctgcta
    39841 atttatcgat gacttttttt cgtttggctc ttgaagtatt tgcctgctct actagcatac
    39901 gttctgcctg aacagtggtt aatcgttcat cttgataatc aactggcaag gagaatagtt
    39961 ctttgatctt ttctccgtat gacatggaag cttctgccct tggtccgatg gaattgttca
    40021 tatttttagg taatccaact acaaaccggt ctacttcata ttgtgctact aattccttca
    40081 accgatcaaa gccaaactct tcttctgctt cattgatccg aatgatctct actccttgag
    40141 ctgtccagcc aaaggggtca ctgatagcaa cacctactgt cttagaaccg acatccaagc
    40201 ccattactct cataaattca ctccatgatt tgagaggtag tccttgacaa ttacttccat
    40261 gatctcatcg cgttcgtgac ggcgaatcaa atttcgagca tcacgataac gaggaatgta
    40321 ggccgggtct ccagaaagca agtagccgac aatctgattg attggattgt agcctttttc
    40381 ttccaaagcc cgataaacaa tcgctaatgt ttcgctcact tcttttttgc gactatcgtc
    40441 aaaaccaaaa cgtacggttt catctgtaaa acccatgatt ctacacctct ttcccgcaag
    40501 gtaattccag cttaccttct acaaccttat tttactcgaa gaaagggaaa actacaaaca
    40561 aatattccaa acttccgaat aaattaagaa acactatttg tatgtttttt tccttttttc
    40621 tgtagcttta tcaaaag
//
LOCUS       DADXTH010000018        39293 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.018, whole genome shotgun sequence.
ACCESSION   DADXTH010000018 DADXTH010000000
VERSION     DADXTH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.018"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            74..472
                     /locus_tag="IWI97_000904"
     CDS             74..472
                     /locus_tag="IWI97_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2462425.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            751..1251
                     /locus_tag="IWI97_000905"
     CDS             751..1251
                     /locus_tag="IWI97_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBC2462426.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1334..1885)
                     /gene="lepB"
                     /locus_tag="IWI97_000906"
     CDS             complement(1334..1885)
                     /gene="lepB"
                     /locus_tag="IWI97_000906"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBC2462427.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2037..2264)
                     /locus_tag="IWI97_000907"
     CDS             complement(2037..2264)
                     /locus_tag="IWI97_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBC2462428.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2261..2779)
                     /gene="msrA"
                     /locus_tag="IWI97_000908"
     CDS             complement(2261..2779)
                     /gene="msrA"
                     /locus_tag="IWI97_000908"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBC2462429.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2799..3512)
                     /locus_tag="IWI97_000909"
     CDS             complement(2799..3512)
                     /locus_tag="IWI97_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBC2462430.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3515..4411)
                     /locus_tag="IWI97_000910"
     CDS             complement(3515..4411)
                     /locus_tag="IWI97_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBC2462431.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4569..5411)
                     /locus_tag="IWI97_000911"
     CDS             complement(4569..5411)
                     /locus_tag="IWI97_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBC2462432.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5543..6196
                     /locus_tag="IWI97_000912"
     CDS             5543..6196
                     /locus_tag="IWI97_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBC2462433.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6259..6786)
                     /locus_tag="IWI97_000913"
     CDS             complement(6259..6786)
                     /locus_tag="IWI97_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBC2462434.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6806..7753)
                     /locus_tag="IWI97_000914"
     CDS             complement(6806..7753)
                     /locus_tag="IWI97_000914"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBC2462435.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7765..9651)
                     /locus_tag="IWI97_000915"
     CDS             complement(7765..9651)
                     /locus_tag="IWI97_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBC2462436.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9811..11889)
                     /locus_tag="IWI97_000916"
     CDS             complement(9811..11889)
                     /locus_tag="IWI97_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBC2462437.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11902..12177)
                     /locus_tag="IWI97_000917"
     CDS             complement(11902..12177)
                     /locus_tag="IWI97_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462438.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12539..12994)
                     /locus_tag="IWI97_000918"
     CDS             complement(12539..12994)
                     /locus_tag="IWI97_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462439.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(13148..14356)
                     /locus_tag="IWI97_000919"
     CDS             complement(13148..14356)
                     /locus_tag="IWI97_000919"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBC2462440.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14468..14800)
                     /locus_tag="IWI97_000920"
     CDS             complement(14468..14800)
                     /locus_tag="IWI97_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBC2462441.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14948..15823
                     /locus_tag="IWI97_000921"
     CDS             14948..15823
                     /locus_tag="IWI97_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2462442.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15905..16486)
                     /locus_tag="IWI97_000922"
     CDS             complement(15905..16486)
                     /locus_tag="IWI97_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBC2462443.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16492..17751)
                     /locus_tag="IWI97_000923"
     CDS             complement(16492..17751)
                     /locus_tag="IWI97_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBC2462444.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17753..18799)
                     /locus_tag="IWI97_000924"
     CDS             complement(17753..18799)
                     /locus_tag="IWI97_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462445.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(19034..19309)
                     /locus_tag="IWI97_000925"
     CDS             complement(19034..19309)
                     /locus_tag="IWI97_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBC2462446.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19552..20862)
                     /gene="der"
                     /locus_tag="IWI97_000926"
     CDS             complement(19552..20862)
                     /gene="der"
                     /locus_tag="IWI97_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBC2462447.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(21044..22273)
                     /gene="rpsA"
                     /locus_tag="IWI97_000927"
     CDS             complement(21044..22273)
                     /gene="rpsA"
                     /locus_tag="IWI97_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBC2462448.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22408..23088)
                     /locus_tag="IWI97_000928"
     CDS             complement(22408..23088)
                     /locus_tag="IWI97_000928"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBC2462449.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(23137..23772)
                     /locus_tag="IWI97_000929"
     CDS             complement(23137..23772)
                     /locus_tag="IWI97_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2462450.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23838..25241)
                     /locus_tag="IWI97_000930"
     CDS             complement(23838..25241)
                     /locus_tag="IWI97_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBC2462451.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(25228..26259)
                     /locus_tag="IWI97_000931"
     CDS             complement(25228..26259)
                     /locus_tag="IWI97_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462452.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26290..26511
                     /locus_tag="IWI97_000932"
     CDS             26290..26511
                     /locus_tag="IWI97_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBC2462453.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26676..27413)
                     /locus_tag="IWI97_000933"
     CDS             complement(26676..27413)
                     /locus_tag="IWI97_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBC2462454.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27566..28906)
                     /locus_tag="IWI97_000934"
     CDS             complement(27566..28906)
                     /locus_tag="IWI97_000934"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBC2462455.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(29143..30666)
                     /locus_tag="IWI97_000935"
     CDS             complement(29143..30666)
                     /locus_tag="IWI97_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBC2462456.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(31044..31655)
                     /locus_tag="IWI97_000936"
     CDS             complement(31044..31655)
                     /locus_tag="IWI97_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2462457.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31778..31903)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31985..32701)
                     /locus_tag="IWI97_000937"
     CDS             complement(31985..32701)
                     /locus_tag="IWI97_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBC2462458.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32707..33276)
                     /gene="scpB"
                     /locus_tag="IWI97_000938"
     CDS             complement(32707..33276)
                     /gene="scpB"
                     /locus_tag="IWI97_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBC2462459.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33260..34057)
                     /locus_tag="IWI97_000939"
     CDS             complement(33260..34057)
                     /locus_tag="IWI97_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBC2462460.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(34027..34398)
                     /locus_tag="IWI97_000940"
     CDS             complement(34027..34398)
                     /locus_tag="IWI97_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBC2462461.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34417..35304)
                     /gene="xerD"
                     /locus_tag="IWI97_000941"
     CDS             complement(34417..35304)
                     /gene="xerD"
                     /locus_tag="IWI97_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBC2462462.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35400..35873)
                     /locus_tag="IWI97_000942"
     CDS             complement(35400..35873)
                     /locus_tag="IWI97_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBC2462463.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(35978..36841)
                     /locus_tag="IWI97_000943"
     CDS             complement(35978..36841)
                     /locus_tag="IWI97_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBC2462464.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(37037..39136)
                     /locus_tag="IWI97_000944"
     CDS             complement(37037..39136)
                     /locus_tag="IWI97_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBC2462465.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        10850 a         8186 c         6666 g        13591 t
ORIGIN      
        1 tgaatctaaa tttttgacag cgtttcctaa ataagagatg atagacgaac aaaacagaaa
       61 ggagacgaaa caaatgaata gtatggtttt tgttaatttt cctgttgctg atgtcaatcg
      121 ttccgttgct ttttatgaaa agctgggttt taaaaaaaat gaagaatttt ctactgaaga
      181 agctggttca atggtatggg atgacacttt ttggataatg cttctctctc atgattttta
      241 taaaaaattt ataaaagata aagaaatagc tgataataaa aaaacaagtg gtgctttgat
      301 ttcgtttagt gttgaaaatg cggatgaagt aaaaaaattt gctcaaattg caaaagaaaa
      361 tggtggtact ttccacgaag tagatatggg aatgcctgaa gaagaaatgt tttcattaga
      421 agtccaagat ctagacggaa atacattaga acccgtatgg atgaagatgt aagcagataa
      481 ctaatctgac gttttttata acaaaaaaac aaaggcggat gatacgataa gtattctccg
      541 cctctatttt ttttattcgt acaatctctt ttttacagtt tataaaagca gtcttctcac
      601 ccttttttta attgttagat gccagtgtat gtaaatcacc aatgtgtcat gacacattgg
      661 tgattttatg gtatcctttt attattaact gacaaaggag agaatatttt gaacactttg
      721 attgcaaaag acgtttttag attgcccgaa atgaatgcaa gattgttgtc gacctctgct
      781 cttttattag cccttgatgt catcttatac aagatagcga ttggtcctgc ttattttcaa
      841 atcacattcg gatttttaag tatggcgctt atgggttatc tatatggacc gatctgggct
      901 ggtttgcttg aaatgttgtc gaacacttta aatttcacga tatttggttc aggaacgttt
      961 caagctgctt ttcttatcac ggctttcctt ggtggattta ttaatggctt atttttatat
     1021 cataaaaaga taaacctgcc ttccgtcatc gctgcccaat tattgattat gatcccagtc
     1081 agccttttta tcaattcttg gctgatgtct gttttgtttg gtgcagattt caaagtaatt
     1141 tttctaggta gattcatcag gaatatgatc caaatcccta ttcaaataat ggttgtttat
     1201 tcatttatca aaactttaga ggctagaaaa gtgtttaaac gagctgtttg attttacgta
     1261 ctaaggtagt aaactgattt tggattaaac aataaagcgt gcagtctcaa cagactgcac
     1321 gctttattgt ttctcactgg atccatttta tttcatcaag tggataatag acaaatcttg
     1381 ctttacctag aatatcagtt ttattgatcg tgccaaacga acgactatct tttgaaactc
     1441 ttcgattatc tccaagtaca aaataattat cttccggaag cttggattgt cccgttagct
     1501 cttgtagtgt aaagtccgtc gtataggatg catttgcatg atctgatttc aggtttttag
     1561 ttagataagg ttccttaact actttcccat tgatcttcaa ttgatcattt tggtagctga
     1621 cattttctcc tggaagaccg attactcgtt tgatataaat cgtgccatct gctaattgaa
     1681 aaacaacaat atcaaatcgt ctgatctccg aaaatttttc catcacaacc atatcacctt
     1741 gcttcaacac gttttccatt gaattgcctt ccactggtac cgggatgaat aaaaatcccc
     1801 gcaacatgaa cgcaaggaat acagaaacaa gtaagtattt gaatacaagc cagaagcgat
     1861 taatatagcg ttgttttttt gtcaatctct atcaatccat ccctcttttt ttcattcttt
     1921 ctttattata agaaaaatat caaaaaaatg cgactagata gctgaaataa aaataattct
     1981 ttcaattagg ctattggaca aaaaactttt ttgatcataa agtacaaaac tatctcttat
     2041 atgtcatgat tattttctaa atatttttcc catgcttcgt caaagatatc catggacggc
     2101 aaatagtcga cattcagttc cagataatta gaaatttctt cgtagctaga gctgtgctta
     2161 ggaaattgga tgtctttacc ggcttctgta gcgaaaacag ataaagcatc atgtgtgcca
     2221 ccgcctcgca gtgtcattaa ataatgaaaa aagcttcgat tcacccttcg ttcctccaat
     2281 tttcttcgat aaaggcagcc cggcgtgcat ggtttctttt aaaattttca ggatgctttt
     2341 tataatactc ttgatggtct tcttctgcta gataaaatgg ttgggctggt tcgatttttg
     2401 taacgattgg ctgttcgaaa cgtccacttt cagctaactg tttcttactt ttttcagcaa
     2461 tgatccgctg ttcttctgtc aaataaaaga tcaccgggcg ataattgtca ccgcgatcct
     2521 cgaattgtcc gaaagcgtca gttggatcag tttgctgcca gtaaagttct actaattgtt
     2581 catatgaaat caatgttgga tcatagataa tctccactgc ttctgtatgt ccagtcgttt
     2641 ggctttttac ttgttcataa gtcggatctg gtacatgccc tccagtatat cctgacatca
     2701 ctgtatggat accaggaagc gtgtcgaaag gctggatcat acaccagaag cagcctcctg
     2761 caaatattgc tcgttccatt atgattcccc ctctaatact actcatccga tgatttttta
     2821 ttttcttttg tcttgctttc agtactgctg gtgctagttt tgttggtatt tgagttgttt
     2881 gaagcatcag aggtatcttt tggcaaatat agactaaacc gaatgtcatc atttactaag
     2941 tcgattttat ctgctttaat atacatcccg ttttgcattc ggaattgatc caacctaagc
     3001 atgatagtca gatcatctgt attgacttcg atccaatctg gtaatttata gtttctcttg
     3061 atcatattca tcacatcttt gattggcaaa ctcaatgttc cgatagaaag acttctagct
     3121 tttagttgga catttccgtt ttccatcaca tacggatcaa aatataaata gaaatcgata
     3181 ggaaaaccta gtacttcgaa agtaccattg agtagtgctt cattttccaa ataaaattta
     3241 tattcaatat cactatcttt ttgatactca ctaaggaaaa agctgatgat ttgattgact
     3301 ttttctttat ttgaattgat cgttaagaca ggatctcctt gtctttccgt aagagctggt
     3361 atttctggga gttctggttc ccgattagca aaaatacggc tgcctaaaaa tacggtcgaa
     3421 ccaagtacta ccccaagaag gataaggaag gcgaatttcc agtaatttgt tttctttgtt
     3481 ttgtttgaag atacgtcaga tcttgttttc atgtttattg atcatcctcc ttctctaacc
     3541 attggttttg tgtctcgatc agctgatctc tcactgcatt tgccatcaat tgataaccta
     3601 aattatttgg atggaaatga tcttcttcat agagcacatt atttttgatg ccatttagat
     3661 tttctgtcgt gctagttgtt tgatctgttc cttcttctgt gacacctact tcctgattca
     3721 atccttgata aagcaaatca ttgattggga taaagtagca gttcggcatt ttttttgtca
     3781 ccgtttctgt tcctttgttc caattgttaa caattgtctg catgtcagtg atctcaggaa
     3841 agttcaaata aaatggattg tagatgccta aaatgtaaat aggtgcggtt ttattgaggg
     3901 atcgaaattc ttccaatagt tctgtgacat tttcctgata gtttttcagt ggctttttaa
     3961 aggaattgac cgttaacccg aataaattat tttggataac tttcatcaaa tcattaccgc
     4021 caacagtcag tgtgataaaa tcagctgttt tgatattgtt tcgaatttct tcttttgttt
     4081 ttactcgctt taagatctgg tcacttcgtt caccatttac accatagttt tctgtttcaa
     4141 ccgcggtcaa attataacgg tcttttagat catttgcgac aaggggaacg aatcctcctt
     4201 gcttggtttg atctcctact ccttcagtca gcgaatctcc gatagctatg tagtgaagtt
     4261 tttctttttt atttggatct tctctcacag tagttactgg tttcattagt gggccagctt
     4321 tcggtataga agcagctaaa cctgcataga tgatgatcgc aattaataat gggaacaaaa
     4381 cgaggatcaa atgtcgtttc gttaatttca ttttttcacc tcttgtattc gattggttct
     4441 aatgttatca aaaattttat caaaaaggta gtctttatga aaaaagaaaa caattatttt
     4501 cctaatttta taaagaaaaa gagaataagc tgcaagcctt tcaggctgag ggcttatcct
     4561 cttttcgact aatctacata atacatgatt gcaaacgcgt ctttaccagt atgcgtagcg
     4621 ataaccggat tcgtatgcag tacagggata tgcatgtccg ggaataattc ttgcaaaccc
     4681 tctttgaatt cttgcgccaa ttcttttgca tccgcatgtg aaataccgat ttgtcgcaca
     4741 tttggcaatt ctttcagttc atttttcaaa ttattaaacc atttatcgaa cgttttcttt
     4801 cctcgcccct tagttacagg gattaattca gaatgggcga aattcatcac cacgcgtatg
     4861 ttcaaaacac tagacagcaa accagttgtt cggctgattc ttccaccttt taccaagtta
     4921 tctagtgtgg atattccgat gtataatttt gtattgtctt gtactttttt gacttcttca
     4981 aggattttct ctactgtttt accttctttt gctaattcag ctgctcgaat tacctgaaaa
     5041 gataatcctt ggtcagtggt atcactgtcg ataaccgtta ctttcgtttt tgttagatta
     5101 ctggcttgtc ttgctgcttc tactgttcca ctcaagcctt ttgtcatatg gatcgaaatc
     5161 acttcactgc cgtcttctcc tagctcatca tacatcgaga tgaattctcc aattggtggt
     5221 tggcttgttt tgggcagatt ttttgctttc gccatcattt ccataaatct ttcgccgtct
     5281 agttggtcat catcgggata tactgttccg tcgatcatga tggatagcgg catgacacga
     5341 atgcctaaag catcgcgtac atttttttcc atcgtacatg aagaatctgt tacgattttt
     5401 acatttgtca ttattttctc tctctctttc tttaacaaga tgccttattc atgttaaact
     5461 acatgtaaga caatttctat ttttagtata acagacaaat aatgattttt cattaattac
     5521 acctagtaaa ggagccgttt tcatggaaaa aacgaaattt aatcgaacgt atgaaatatt
     5581 aaatgaagtg tttaatgcag tcacccatgg aattgggact ggattgagta tcgcgggact
     5641 agtggttctt ttagtaaaag gtgcccactt gcattcctcc ctccatattg tctcttatgc
     5701 tatctatgga tcgatgatga ttcttttatt tttaacgtct actttgtttc attcattgat
     5761 ttttacaaaa gcaaaaaagg tgttccaagt attcgatcat agttcgattt ttcttttgat
     5821 tgcaggaagt tatactcctt tttgtttgct gagtatccga ggatggctag gttggacttt
     5881 atttgtatta atctggttgt ttgcgattct tggcattata tataaagcgt tgacgttgca
     5941 taaacgggag agagtatcaa aagtatctac gatcatctat atttttatgg ggtggctctg
     6001 tgtcgtggct gctgttcctc tttatcactc tcttggaatg actggtgtta ttttgatggt
     6061 agcaggcggg ctttcttaca cgataggcgc attcttttat agcttgaatt ctatccgcta
     6121 tatgcatgtt gtttggcatt tgtttgttat gttaggtgca ggttttatgt acttctctgt
     6181 tttattcttt acttaataaa aaaaataaag ctatggatgg gacagctcta aataacagct
     6241 agtctatcca tagctttttt atatcgtcaa ttcgcctgtc ccgcttttta caggattctc
     6301 attacgctga tacgtttcaa attgatgcgg ataacgattt ttttcatcca tttctccttg
     6361 actagtattg atcagtgacc aatcagacca gtcaacaggt ggaaaataag catctccatc
     6421 gaatacatga tggatgactg tacggtataa tatcgtacaa aatggcagaa attcttgata
     6481 aacagctgac cctccagcga tgaaaaagat accgtctgtt tctttttcca gagcaagcac
     6541 gtcttccaca ctatgcatga catgcacgcc ttctgcttta taggaagaat ctctcgtcaa
     6601 aacaatcgtc tgacgattcg gcaacggacg gctacccatc ccttcgaatg tttttcgtcc
     6661 catcactagt gtatttgctt ctgtcatttg tttgaagaat ttcagatcat ttggcaaacg
     6721 ccaaggaaga cggtcttctt ttccaatcaa tccgttttca tcctgtgccc aaattgcagc
     6781 taacattttc tcatcctttc tgattctaaa cagcgatagg agctttgatc gccggatgtg
     6841 ggtcatatcc ttctaattgg atatcttcca tctcaaaatc aaatactgac tgcttttcct
     6901 gattcaaaac taaagtcgga aatgcgcgta tttctcggct caattgttct ttcatttgtt
     6961 cgatatgatt gatataaaga tgagcatctc ctaatgtgtg aacaaattcg ccaaccgcta
     7021 aacctgtttc atgtgcaatt aggtgagtca gcaacgcata acttgcaatg ttgaacggaa
     7081 cgcctagaaa cacatcccca ctgcgttgat acaattggca gcttagccgt ccctcttgaa
     7141 cataaaattg aaacatcgta tgacaaggag gcaaagccat gctaggaaca tcttccggat
     7201 tccatgcaga aacgatcaat cttctagaat caggagtctt tttgattgct tcgatcactt
     7261 gtttgatttg atcgataaag ccaccgtctt ttgtttccca gtgacgccat tgatagccgt
     7321 agatgtgtcc aagatcgccg tatgtttcag caaaggaatc atcggttagg atcttctcgc
     7381 aaaaaagctt gctctgcttt ttatattctt cattgaattc aggatctgtc aaacatctat
     7441 ggccaaaatc agtcatatct ggtccattat agtctggact tttgatgtag cgttcaaatg
     7501 cccattcatc ccaaatatgg tttttatttt gtaatagata gcggatattc gtatctcctt
     7561 ttaaaaacca aagcaattcg cttttgatca atccaaaagg tactcgtttc gttgttaaca
     7621 aaggaaaccc tttttgcaga tcaaaccgca tttggtatcc gaataagctt aatgtgcctg
     7681 tgcctgttct gtcttcttta cgatgccctt cttccaacaa ttttcttcct aagtcaagat
     7741 acgcttgttc catccttttt cctcctaatc ttctacccat tcactcaagt attcccaccg
     7801 tgccattttt tcttcaagtt gtgcttctgt ttcagatacg tcgttttgca attcttgtaa
     7861 tcgagtgaaa ttatctcctt gatgattcat ctcttcttgg agcaatgaga ttttttcttc
     7921 aagttctgca atctcatctt caatcgtttc ccattctttt tgttccatgt aagataattt
     7981 cttcttttca gcaggttcct tgctctgtat ttttggtttc gctttttcag caggctgctg
     8041 ggtctctttt tgcttcagta aataactgga catgtttccg taataaagtt cttgttttcc
     8101 gtttccttgg aatactaata acttatctgc taccttgtcc aagaaataac ggtcatggga
     8161 aacagtgatc acagcacctg gaaaacttct aaaataatct tccaaaatcg tcaatgttgc
     8221 tatgtccaaa tcattggtcg gttcatccaa taataaaaca ttaggttgct gaatcagcag
     8281 tttcaacaaa aataaccggc gtttttctcc gcctgaaagt ttgcctatca gtgtcccatg
     8341 cataaatctt ggaaataaaa atcgttcaag taattctgct actccgattt gactgccatc
     8401 tgctgttttt gcttcttcag cagcttcttg taagtaagca atcatccgtt tatctggtgc
     8461 catttcttca ttttcttgtg tataatacgc aagatgaacc gtttcaccga tagacattgt
     8521 accgctatcc aggggaattc tgccagctag aatattaagc aatgtcgatt ttcccgcacc
     8581 atttttacca gtaataccta gtctttcccg agattgaatc aataaatcta aatgttctag
     8641 cagtgtttgg tcatttatac ggtaactgcc atctttgatt tccagtactt ttttccctaa
     8701 acgttgtgtc gctaggttca gctctaaatc atcttcttgg tttacttggt acaagttctc
     8761 ctttagatct tcgaaacggt tgatccgtgc ttgctgcttg gtgccacgtg cctgtacgcc
     8821 agccctcatc caagctaatt cctgtttata aagccgcttg cgcttttctt cttgttctac
     8881 ttctacacga tcacgttctg ctttttctaa gacgtaagct tcataatttc ctttatactc
     8941 gtataatttt ccaaatgaaa gttcgaagat tcggtttgct actctatcta gaaaataacg
     9001 atcgtgggta accatcaata gtgcgccacg gtattgtttc aaatagtttt ccaaccactc
     9061 gatcgcttca taatctaaat ggtttgttgg ttcgtctagt aatagtaaat cgggttcatc
     9121 aatcaacact tgcgccagac taactcgttt tttctgacca cctgataaag tactgatttt
     9181 tttatctaat tcactgattc ctaatttttg caaaataatc tttgcgttgg tatctgtagt
     9241 ccaagcatct tctttattca tttgttcttc tgctttagca taacgttttt gcacatcagg
     9301 gtcttctccg ttttccccta gttcgatcaa tgcttcctca tatgctcgaa cagtttggat
     9361 caaggggctg tttccttgaa agactgcttg gagaacagtt aattcatcgg aaaattcagc
     9421 tgcttgagaa agataaccaa tacggtaatc agttggataa aacactgttt gccgatcacc
     9481 gtcgctgcta tcgataccgg caataatatt taagagactt gttttacccg tcccgttcgt
     9541 cccaatcaac ccgatacggt ctttttcgtg gaccaaaaaa gaaatctggt caaacaaatc
     9601 tttttcacca taagtttttt tgaggtcagt tacttttaat tctttcaaca tgttccctcc
     9661 ttatcttgga tattgtatca taaaaatagt gaacttcagg aaaatttttt tggtatttgt
     9721 tctttttgtg ttctcaaaaa aagaacagga ccgattcgat cctgttctga tatgttttgt
     9781 tttttataaa acttgaaaat ttttaccttt ttattttatt ggactcgttt tttttgactt
     9841 tgctcgaaac gcaaatcgga aagaaaagtt atcagatggt ttgttcatcg tataagattc
     9901 taacaaattg atattcctgc gatagctgtt cgcagaaaac gtcgtgattt tttcagcaac
     9961 ttcgtattca tcaatgattt ttctttcaag ataataacct cttagcattt ttttcgtaaa
    10021 cagactatct tgctggatca tccacgaaga aatcaaattc cccgagttca tttttattcc
    10081 ttcaagtttg cgttgctgga taaaaaattg aaccatcgtt tcatcataaa tatcagtcaa
    10141 gttatctaaa ctttcaataa tcaactgagt atttttttga tatgtctctc tgatttcttg
    10201 gatatcttca tcactaataa atgtctgacg atcagcatat cttctttgcc aaaacatttt
    10261 cggatggaga acgattcgca ttaacctacg gacaacaatg aaccaaaagc cgataaaaga
    10321 caaaatctgg cttgtgatag aatgttcgaa tcgagaaaga aaacgggagt aaaaacgata
    10381 accattagaa gaaagcttgc ccgaacgata actttcatcc aatccatccc gttcgatcga
    10441 caaaatcagt gcttgtattt cttgtacttc ttgcttttca tcatcggtta aatcttccaa
    10501 gtacagatca cgtatccgtt cttgataatt ttctatcact gcttcagtgg ctaaaaattc
    10561 ttttgtatta cgatcaattt cctttatttc ttttcgcaaa acttcaacga cctcttctaa
    10621 aatcatcaaa gcattcaaat ccgtgctttt cgcttcctct gtttccgtca gcataggcaa
    10681 gacaatgatc cctatgacca aagtcactaa gatgacaccc gccgtcagaa acaaaagcaa
    10741 agaccgctgg taaaacatca tattattgat tgaaaatggc aaaataaaaa tcgtagccaa
    10801 actgactgtt ccctttacac cgccaaatgt taagatcaag atctcgttca actgtttttt
    10861 gaacttttta ctgccatctt tgaatacata aaaaagactg atagaaatga atctgatcac
    10921 aaaaagcatg acagagatta atacgataat cgctaataaa agaccgtttg gatataaacc
    10981 gtctccccat actggcgaga acacttgcgt caattcgatt cctaaaaata taaatactaa
    11041 tgcattcagt gtaaaagcaa tagttgtcca tgtactgtgg gacaagctcg acaattctgc
    11101 atcaaacaca gagattttcc taaaaccaga agcttggaga atacctgctg ccactgcagc
    11161 aatgattcca gatacatcaa atgcctctgc caatacatag gctagaaaag gaagcaaaag
    11221 ttcgatcagc agataccctg tgacatcttg tgctgatgct ttttcgatca aatgaattat
    11281 tttttgtttg aaccagacaa gcaaaaagcc gatcaaagct cccccaatac tagaaactac
    11341 tagactcatc cccgcgttta ctgctgaaaa agatccggtg atcagcgcac caagagcaaa
    11401 ctggaaggca gtaacaccag atgcatcatt caaaagacct tctccttcca aaatatgcat
    11461 cgccttttta gggatattca ctcgtccaga aagagaactg actgctaccg catctgtcgg
    11521 acctaacgct gctccaaaag caaagcaggc agcaagtggg acacttggca acaggaatga
    11581 taacgtcgca cctaccccga caagggtcaa aatgactcct ccaaatgcca agaacaaaat
    11641 tgtcccgaaa tttttgagaa tcgaagaaat atctgctttt tctccttctc taaataacaa
    11701 tggtgcaata atcataacta agaacaattc aggttcaaaa tcgattgatt gtccccactc
    11761 tgtcagacct aataccactc caaggaaaat ttggatcagt ggagctggaa tcattggcaa
    11821 aatgcggcta gctatgttcg ataaggtgat cgttactgca aatacaataa ctaactcagc
    11881 aaattccatc gtccatcccc tttatttttt attttgtgta agcattttta aaaattgttc
    11941 ttttttcttt aaaattttgg tttcgactac ttcaacctgt gtttggttcc cctctaattc
    12001 acactgtaca cgttttgact ccaaacgaag cagatcatgt ttcagacgtt ccaactgacg
    12061 atacatcaat atatttcttt tacgcaactt acaaagtatt tgacggcggt catccatcga
    12121 aagggcgcca tagcgatcat gcaacagttt tttttgttgt tcaaaactct ctctcatttt
    12181 ccagatctcc tatacacaaa cttccatcat tatagcaaag ttctttttgg atgaaaaata
    12241 tttcattgtt tcatcgttta ttgctagtta gaaatttcca taaatttttc aacgcctcat
    12301 tctattccaa atagtatcag atatcaagta aaacttaaga aagataaaag tttctgacaa
    12361 aaggatgttt actttcccat tcctgccttt cttgttcttt tatcacaaca aaaaaatggg
    12421 gctgcgacaa aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc
    12481 ctatcagtag taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt
    12541 attcgaaaaa ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca
    12601 agttacctgt tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc
    12661 ctttatctag atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca
    12721 ttcgctcgat atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta
    12781 ggttgatatc atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag
    12841 cacacggatg gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt
    12901 gtttgatcca aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa
    12961 aataaaattt tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt
    13021 tattcttgta ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt
    13081 tcttctaatg acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta
    13141 gtgtttttta aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt
    13201 ttattctttg ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac
    13261 caggtttttt tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg
    13321 acttgatagg taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa
    13381 aatagatcat caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga
    13441 atggttccaa agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt
    13501 taatcatttg gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta
    13561 gatccagttg gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg
    13621 gtaattcagc aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt
    13681 agcactctgt ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct
    13741 tgatgaattc cacatttata cgttctacag atattttacc taatagctcg tggttttctt
    13801 tgatggcgta gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc
    13861 cgcgcatcat tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca
    13921 agatctgttc ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt
    13981 tcaaagcaaa cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa
    14041 cgaattcgac atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg
    14101 tgatctcata ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat
    14161 ctatcgtttt tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca
    14221 catcatggat tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt
    14281 catatcctgc attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta
    14341 attgttttaa tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact
    14401 cagttgcatg gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc
    14461 atcttgttta ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca
    14521 tgtttttttc aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa
    14581 cgaataaaac atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc
    14641 ttttttctcc ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc
    14701 gagccatctg agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca
    14761 cttcctgctg catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat
    14821 cttaccaaaa ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg
    14881 ctacatttag gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg
    14941 aattcatatg gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag
    15001 tatttatgca tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt
    15061 ctcagggatc accttgatct taagagcttt atttggtatt gatccagctt atacgacttt
    15121 attaatcaac atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc
    15181 ctatactgtt ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc
    15241 actagaaatc aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc
    15301 aggtgtagga agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat
    15361 cgctcggatt ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga
    15421 tatcatcgtc ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt
    15481 gattgtttcg tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc
    15541 aaaaggtcta cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg
    15601 tctgcaaaga ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa
    15661 aatcatctat atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga
    15721 gattgacgaa aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt
    15781 tacttatttg aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata
    15841 ggctatgagg aaagattcca catccagctg acctttgtct tttttagtac tatactattt
    15901 ttaactaatt gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct
    15961 ttcggttaac ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt
    16021 gatcgatttg agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc
    16081 gttcaattaa ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt
    16141 cttctaagat agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat
    16201 ataaaggctt tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt
    16261 cttttccctg caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca
    16321 gtcctcgatc cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat
    16381 gattggcaag ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga
    16441 ccatccacgt taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc
    16501 tgttaggtca tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata
    16561 agtcaataat tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc
    16621 tttcaagaaa tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc
    16681 aacggtaaag tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt
    16741 agcagtttct tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa
    16801 gtaaagatta cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt
    16861 cagtaaaagt tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag
    16921 gtgataaagt tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc
    16981 ttcttcaatc atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata
    17041 atgcggatta acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa
    17101 ttgaagattc gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc
    17161 ttcttctaaa ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa
    17221 acgttcctgc atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata
    17281 aaccactgct gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg
    17341 gatcaatggt tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc
    17401 agggattccg ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc
    17461 actttgctta tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat
    17521 tgcaatttct gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg
    17581 ttcaaaaatc tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc
    17641 agctaatata tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga
    17701 caagtcttca tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc
    17761 ctcttatctc actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt
    17821 ttggatgttg gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt
    17881 tttctgccaa aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt
    17941 ggattggata gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag
    18001 gatatgcttg gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag
    18061 cagaaagtag atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat
    18121 ttgacaggtt ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa
    18181 acggccgagt cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac
    18241 gatgatcaca aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca
    18301 cctgccgctc gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca
    18361 gcactacacg atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag
    18421 aacggatcga tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa
    18481 aacctgccag atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt
    18541 cgggatagcg tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag
    18601 tgttggcaca aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat
    18661 ctgcaaacaa atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa
    18721 gttctcttag ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta
    18781 cttgataatc aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa
    18841 gcaaattcta gaaaagataa gagcaaagct cacctcataa cttgcaaata aataaaaatt
    18901 aaataaaata catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt
    18961 tattcctttt ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc
    19021 tttttcaact gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta
    19081 ctttgctagc tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg
    19141 cgcgttcgcg aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta
    19201 aagcatcttg aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta
    19261 aatcagtagc tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca
    19321 cctcctccac aagagatatg atagaaatat catttagtta aaactttctg agtggtccag
    19381 aagtttaaaa agaaatattg attaacttca atattctgtt atcacgatat catatgaacc
    19441 gcttaatagc aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca
    19501 aacgaaactg tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct
    19561 cctacgaggg ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt
    19621 ttctaagaaa cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa
    19681 tgtaggtggt ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc
    19741 tgttggtgtt ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga
    19801 gatacgaaga ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg
    19861 ttgtttagtc actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc
    19921 acgaatctct tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt
    19981 attcacgacg ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc
    20041 ttgttcccga atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat
    20101 ggcacgcatt gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt
    20161 acgcatacca gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt
    20221 gtcgattgcg tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa
    20281 aatcgcattg atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat
    20341 cgtgtcgtcg tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat
    20401 atcaccaatc cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga
    20461 ataaaattca tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat
    20521 cactggttta ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc
    20581 ttcgcgtccg ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg
    20641 atgcttgatc tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac
    20701 gctgaattct cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg
    20761 agtatcttcg acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc
    20821 gacgttcggg cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa
    20881 gtttccaatc tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat
    20941 taaacgaagt atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag
    21001 acaaaagcag ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat
    21061 cggttgcttt ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa
    21121 atccagtatt ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag
    21181 ctggtttttc ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct
    21241 ctaatacttt cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat
    21301 gtttatgtga aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa
    21361 aggcaccgaa gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt
    21421 ttgtatcgat ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt
    21481 cttgttcagg attgatagac aacactttga catttacatc atcattgaca gccaatacat
    21541 cacttggttt tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc
    21601 cgcctaggtc gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat
    21661 ctccctcatg gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta
    21721 ccgctttatg cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc
    21781 tcaatgtttt gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg
    21841 cagggacaaa accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa
    21901 caggtgcttc gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt
    21961 tagcatctaa acggcgtttt gaaagtagat agctgccgtt ttctttatct ttgccgatcg
    22021 tactgataac gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta
    22081 ccggcaaagt tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc
    22141 cgacaatcac ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt
    22201 ttacttcttg tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat
    22261 caaattctgt cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt
    22321 tttagtttaa ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa
    22381 caattgtttt tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac
    22441 agcttttatt gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc
    22501 atcctctgct tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg
    22561 tatctctttc gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa
    22621 acgtctttct gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg
    22681 taatacagct gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt
    22741 tcgttggaca gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc
    22801 ttttgtgaca tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg
    22861 ctgtccatct tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc
    22921 ttcttcatct gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat
    22981 ggcaccggta tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact
    23041 tttcccagat gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct
    23101 ttttctcaga aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc
    23161 agggtaaagc ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt
    23221 gatccctgca cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga
    23281 accggctgca gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc
    23341 ttgataagtt gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga
    23401 cgttacacct gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt
    23461 tgaagtctca gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga
    23521 agtttcagaa gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata
    23581 agcacctgct ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa
    23641 tgtatttcct ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc
    23701 gtagattggt tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc
    23761 ttttctgctc actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag
    23821 tgtcttgtcc taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt
    23881 tcccaatgaa gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt
    23941 gctccatcaa tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct
    24001 aagatgaatt ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg
    24061 aattcttttt ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac
    24121 tgctggatca gctccatcca ttttttttgc agctcatcaa agggagcttg ctctgtggca
    24181 tattccaaat aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa
    24241 tggatacgtt catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg
    24301 cctgctcgac cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc
    24361 acataacgga tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac
    24421 tgcagctgat tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca
    24481 ccgtgatagt atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct
    24541 ttttttgttg cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt
    24601 tcttgttctt tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat
    24661 tgtctgatct cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa
    24721 tctgttgctg tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt
    24781 cctaactgtt gatattctgg acggaaatca actccccatt gagacacgca atgagcctca
    24841 tctactacat ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc
    24901 aacatttcgg gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt
    24961 ctttcagatt gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt
    25021 aattgcgtca cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct
    25081 tccattaaat accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata
    25141 gcgagagtat cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat
    25201 gaatcaaatc caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct
    25261 caatagatag atttgataca aacgaaattc tccataatct aattgtccat ctacattcca
    25321 ttcgctaaag cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc
    25381 tgcagagaga atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg
    25441 atctgttact gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc
    25501 attcataaac atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga
    25561 atcgatcaaa gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg
    25621 gatacatctg ctttgttgca gataaaccgc tgactcatca ttgttttttg ccagctgata
    25681 aggaagcagc cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc
    25741 ttctggtatc tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc
    25801 acgtggcaat tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt
    25861 ctctgctgga agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa
    25921 tttaatgagc cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt
    25981 atgttctatt tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt
    26041 ttcatttatt tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc
    26101 ctgcttcaat tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat
    26161 aagtactgaa gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt
    26221 gtgtcctgat gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga
    26281 tggttagaaa tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt
    26341 tgtcaaatcg aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc
    26401 caagaaccag aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa
    26461 gcgtatcaaa aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg
    26521 aaactttaaa aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc
    26581 ttgttccgtg aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac
    26641 tagttttttc acggacccta taggacccat ttatattatt tcaacttttt cagccagttt
    26701 tccaatgtat tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct
    26761 cctttttgct caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac
    26821 tctgttcttt ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta
    26881 tcttttgtcg ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg
    26941 tcgggataac ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg
    27001 acataacgac ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt
    27061 ggaaaatctt ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc
    27121 attttgacaa tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa
    27181 ctttctggct ctacgagagc accaggataa aaaataagta atggagactt tccatgattc
    27241 tttttctctc cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact
    27301 tgcgcttctg acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg
    27361 agtaatccta aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc
    27421 caccttttct atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa
    27481 taagagagaa atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat
    27541 atccgccctt tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat
    27601 aaatttccat tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa
    27661 agtcaggagt atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa
    27721 aagtagcgcc gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc
    27781 ccaatagatt tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa
    27841 attcttcata agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat
    27901 ctttcttgaa tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga
    27961 tttccaaact tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca
    28021 cgacagcttc accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa
    28081 aatcaaatac ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg
    28141 catctttcgc gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat
    28201 cgaattcgtg ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag
    28261 ctgcatcagg aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg
    28321 gtcctgtcaa gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat
    28381 ctggcgcaaa tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc
    28441 ataaattttc ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag
    28501 catcaatcaa agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg
    28561 atccgcgacc aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat
    28621 tattttcgtc ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc
    28681 cagagccaat ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta
    28741 gcttttcact gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat
    28801 gtccttgtcc tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg
    28861 attctaatgc ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct
    28921 aatatgttaa aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg
    28981 acgttttaag cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa
    29041 cactcactga atggagctat tgacttatcc acgtgcttat cgatagccgt ttatcggttt
    29101 cttcattctt atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac
    29161 acctaggatc acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca
    29221 ccacactgaa acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg
    29281 tgtaattaag tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc
    29341 gacgccggca aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga
    29401 agttgaagga atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac
    29461 acttgaagat aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa
    29521 tccattgttc ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat
    29581 caaatcagaa gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt
    29641 gattgctact acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc
    29701 acctaatgga agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca
    29761 gtttgtcaat gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact
    29821 gatgatcatg ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt
    29881 gattacttct tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg
    29941 gttgtatact aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata
    30001 tacaatagaa atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc
    30061 catcgaaatc ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc
    30121 tagacagcct acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc
    30181 tacagccatc atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc
    30241 gtaagtagag ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt
    30301 tcctttgaat ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat
    30361 aatacctaaa aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt
    30421 ttctttagtt agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat
    30481 atcccacagc ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc
    30541 tgcatcgtct acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc
    30601 aataataata aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg
    30661 ttccatcttc ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt
    30721 ttatcaaaaa gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa
    30781 aaagttcgtt tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt
    30841 cattgtagcg tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat
    30901 ttcacatttg caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa
    30961 acaaataaaa cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag
    31021 aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt
    31081 tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg
    31141 atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac
    31201 atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg
    31261 aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa
    31321 aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta
    31381 aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct
    31441 gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg
    31501 aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa
    31561 gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct
    31621 actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct
    31681 cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat
    31741 agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta
    31801 tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact
    31861 ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt
    31921 tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa
    31981 gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat
    32041 ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca
    32101 caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg
    32161 tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg
    32221 gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg
    32281 tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca
    32341 ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca
    32401 gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc
    32461 ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac
    32521 cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt
    32581 ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt
    32641 cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca
    32701 ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc
    32761 aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata
    32821 atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc
    32881 tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc
    32941 tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt
    33001 ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg
    33061 agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc
    33121 tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc
    33181 ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat
    33241 aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat
    33301 aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca
    33361 attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag
    33421 aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt
    33481 cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac
    33541 gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca
    33601 acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac
    33661 ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta
    33721 aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtga
    33781 cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca
    33841 ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat
    33901 attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca
    33961 gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca
    34021 actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat
    34081 ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa
    34141 gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc
    34201 gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa
    34261 gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt
    34321 tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt
    34381 ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt
    34441 ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc
    34501 cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt
    34561 ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac
    34621 taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac
    34681 aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg
    34741 ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc
    34801 aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc
    34861 actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa
    34921 cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact
    34981 tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat
    35041 acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt
    35101 tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc
    35161 gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc
    35221 acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag
    35281 ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc atatcagata
    35341 ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact
    35401 cagacggact ggtttctttt ttctttttca tagcattcct gacagattcc atggaacgtt
    35461 agccgatgat cttttactaa aaaatgataa cgttcttcta ctacttgctc cacttcaagt
    35521 aaaagatcct cttctacttc ctcgatcgtg ccgcagttca agcatagtaa atgatgatgg
    35581 aaatgtttgg cgccttcttt tctcaaatca taacgggcta atccatcatt aaaacttact
    35641 ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc gataaacagt agcaagtcca
    35701 atttccggac tctttttttt cactaaaaag aagatttctt ctgcagataa atgctctttc
    35761 tcattttcca aaagcacaag cagagtagct tctctttgcg gtgtcagctt aaaccccgct
    35821 tcatgtagct gctgttttgt ttttttcatc gccgaaatag cattcatgac catttactca
    35881 accccaacaa tttataataa ttacaaataa aaaaagatat aacactatgt taattaaaat
    35941 tattataagc tagttaagaa aaacatgcaa gggattctca attagatttt ttgactagtt
    36001 ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa aagattacct aatgcgcgct
    36061 taaatgctcc tttgctgata ccaaaagctt tcatgatttc ttctggatcc gatttatctg
    36121 taaagttgat ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt tgtgcatcat
    36181 ctgatatagc ttcatatgct cttggcttca atgaaagatt cagtacaccg tctggtcgga
    36241 caccaatcac tcgacctttt atctgctcgc ctagacgagg ttcattatat cgttcagaag
    36301 gatggataaa cccaagatag aaatcttccg ttaaaacgta cgtacctgtt aatttcaagc
    36361 ggtatgctgt tcctatcaca tctttgtttt tcatttcttc tgttccatat ttgcttaaag
    36421 atttgaatat tttttcatct gctaaactgc cccaaatacg gtcttttgca tcaactctaa
    36481 gggaaatcat caagcgatcc ccttttttag gccatagctc tgtcattgtc ggcaattcat
    36541 caagagatac gacaaagtct ttatctggaa gaccgatatc gacaaacgca cctaaatctt
    36601 ttcgtgtagc agtaacgatt ccaaaaccgt aatgaccgat acggctttta ggaatttgtg
    36661 tagtgatccg attttcatgt ttttgatttt ggtaaccgaa tccttcaact gcttcaccga
    36721 ttttgtgttc accttctgat ttgcttaagc caaaggtttg tccgtttttt tggacaaagt
    36781 agtttttttc attttcatca ataataatac ctgtaaatat agttgctaat aaatcgttca
    36841 tttttttctc cctctatagt gaaaggtttt cttaatacat ttctttgcta tcataacata
    36901 atgttatcta cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg agtatgacaa
    36961 cagttgtgtc atactcccct ttttcgaata aacggtgtta aacggcaggc tcacagcctc
    37021 tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt ttacagttga
    37081 tggttacgat gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat gcagattttg
    37141 ttgaagtggc atatagatta taatagcctt ccccattttc gattctggct ttttctaatc
    37201 tgtaacctcc ttcaacgata tatccacgtt ggatacatgt agcaatcact ttgctttgga
    37261 cacctggtgc ccacgtccat gctggattag atgcatcggc aatatcactt gctctggcag
    37321 atttgattgg ctgattcgct gagtttgaag aggaaggggt cgaggatgcc gcttcttgct
    37381 gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc tgcagctgct gcttgagcgg
    37441 cttgttttgc ttgttcttca gctgcttttt tcgcttcttc tgcttttttc ttttcctctt
    37501 cttctttttt ctctttatca gtgagcatct tatctacttg tttcaattgt tcattcaact
    37561 gagcagatag atctgtattt ttcacttttt ctactgcttc ttttgccgtc ttgtattggt
    37621 cgcgtgttgc tgaatccact acttttccgt ctttaaagac tatagctgtt ttttctttag
    37681 ctgtttgcaa ttcagcatat tgatttttag cttctgtttg tgcttgttga atcaattttc
    37741 caaaatctgt gttatccgtt tcgattgctt ggatctcttt atctgctttc aacttgatgt
    37801 catctgctag atgatcatcg acgatggcag gtgctatgaa ccactcattc actttttgga
    37861 tatctcggat cttagattgg atatcttggt aagttgtttc taactctgaa tatccttttt
    37921 cattcttaag actggataaa tcatttttta gttttgataa atcttgatga atcatatctg
    37981 cacgaataaa ttgatggtga tcatctgtat aaaaggcagc aagtttctca tgaagttctt
    38041 ccatttgttg ctgtcttttt gcttctgctg ctacttcggc ttctactttt tgatgatggt
    38101 cataataagc ccagcttcct ccaccgagta gaagtactgc tgctaaaacg agataaggta
    38161 ctttttttga ttttttcttt tgcgctttat ctggatctgt tttttctaag tttgattctt
    38221 gatgtgtttt ttcagtatct ggcacgattg ttattgattt cgcttctgct tcaaacagct
    38281 taggctggtt ctcttctttt tctatttttg cttcaagatc agtaccctct ttgcttacag
    38341 attgattttc agcatcggat tcactattta tttcatcggg ctctgttgac tttttgtctg
    38401 tgttttcgag tatttctttt ttctctgctt ccggcagttt attcgttttt tccttttcta
    38461 attgtttttc agtaaatact tcagtttttt ctgtgatatc cgtttttacg tcttttattt
    38521 ttactgtttc tttacctgtg gcgttttctc tatcagagtc tttttcagtt tcttgttcct
    38581 tttctggctc tactttgttt tcctgtgcaa ttttttcact gatcattttg agcagctctt
    38641 tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat ataagcggat agtatcgaat
    38701 tttcttcctc attatcgacg atttctttca gttcagctac tcttcttttt tgttttgatt
    38761 caagcgtttc ttcttgattc tttcctgatt cttccgaagt ctcatgaaca gttgctttct
    38821 tctgttcatt tttctctata ttagttactt gatcgtgagc aggtggttct tcgtcttctg
    38881 gttctgcttt ttcgaataat tccattacag attctagagg taaatctttc aattccgacc
    38941 attcgattgg atcgtttaat tctgtatcag gaaagactgc atcttcagtt gatttatcct
    39001 gcatagctgc atctgtattt gtttcttcaa ttgattcttt tatttgctga tcttctgcag
    39061 ttatttgatg accgcagtct ggacagatag tattctgctc catttttttc gatccgcaat
    39121 ttggacattt tttcatcact tagcgatcgg ctcctttact aggtttacgc taccatttaa
    39181 tctatcatat tctatacgtc acgccaatag cttattttta ggatttactt aaaaataatg
    39241 aatgaatttc cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa gct
//
LOCUS       DADXTH010000019        37836 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.019, whole genome shotgun sequence.
ACCESSION   DADXTH010000019 DADXTH010000000
VERSION     DADXTH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.019"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..765
                     /locus_tag="IWI97_000945"
     CDS             64..765
                     /locus_tag="IWI97_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBC2462466.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            762..1883
                     /locus_tag="IWI97_000946"
     CDS             762..1883
                     /locus_tag="IWI97_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBC2462467.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1898..3130
                     /gene="pepT"
                     /locus_tag="IWI97_000947"
     CDS             1898..3130
                     /gene="pepT"
                     /locus_tag="IWI97_000947"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBC2462468.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3262..4170
                     /locus_tag="IWI97_000948"
     CDS             3262..4170
                     /locus_tag="IWI97_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBC2462469.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4205..4477
                     /locus_tag="IWI97_000949"
     CDS             4205..4477
                     /locus_tag="IWI97_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBC2462470.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4488..5534
                     /locus_tag="IWI97_000950"
     CDS             4488..5534
                     /locus_tag="IWI97_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBC2462471.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5785..8394
                     /gene="clpB"
                     /locus_tag="IWI97_000951"
     CDS             5785..8394
                     /gene="clpB"
                     /locus_tag="IWI97_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBC2462472.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8545..9231)
                     /locus_tag="IWI97_000952"
     CDS             complement(8545..9231)
                     /locus_tag="IWI97_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462473.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9209..9703)
                     /locus_tag="IWI97_000953"
     CDS             complement(9209..9703)
                     /locus_tag="IWI97_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBC2462474.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9723..10943)
                     /locus_tag="IWI97_000954"
     CDS             complement(9723..10943)
                     /locus_tag="IWI97_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2462475.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11090..12925
                     /gene="lepA"
                     /locus_tag="IWI97_000955"
     CDS             11090..12925
                     /gene="lepA"
                     /locus_tag="IWI97_000955"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBC2462476.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13121..14032
                     /locus_tag="IWI97_000956"
     CDS             13121..14032
                     /locus_tag="IWI97_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462477.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15028..15279
                     /locus_tag="IWI97_000957"
     CDS             15028..15279
                     /locus_tag="IWI97_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462478.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15362..15634
                     /locus_tag="IWI97_000958"
     CDS             15362..15634
                     /locus_tag="IWI97_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462479.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            15724..15963
                     /locus_tag="IWI97_000959"
     CDS             15724..15963
                     /locus_tag="IWI97_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462480.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            16110..20393
                     /locus_tag="IWI97_000960"
     CDS             16110..20393
                     /locus_tag="IWI97_000960"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBC2462481.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            20492..20857
                     /locus_tag="IWI97_000961"
     CDS             20492..20857
                     /locus_tag="IWI97_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462482.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            20859..21311
                     /locus_tag="IWI97_000962"
     CDS             20859..21311
                     /locus_tag="IWI97_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462483.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            21925..23235
                     /locus_tag="IWI97_000963"
     CDS             21925..23235
                     /locus_tag="IWI97_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBC2462484.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            23258..23569
                     /locus_tag="IWI97_000964"
     CDS             23258..23569
                     /locus_tag="IWI97_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462485.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            23930..25186
                     /locus_tag="IWI97_000965"
     CDS             23930..25186
                     /locus_tag="IWI97_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBC2462486.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            25183..25728
                     /locus_tag="IWI97_000966"
     CDS             25183..25728
                     /locus_tag="IWI97_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462487.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            25729..26229
                     /locus_tag="IWI97_000967"
     CDS             25729..26229
                     /locus_tag="IWI97_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462488.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            26310..26866
                     /locus_tag="IWI97_000968"
                     /pseudo
     CDS             26310..26866
                     /locus_tag="IWI97_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26886..27230
                     /locus_tag="IWI97_000969"
     CDS             26886..27230
                     /locus_tag="IWI97_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462489.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            27249..28160
                     /locus_tag="IWI97_000970"
     CDS             27249..28160
                     /locus_tag="IWI97_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2462490.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            28239..29231
                     /locus_tag="IWI97_000971"
     CDS             28239..29231
                     /locus_tag="IWI97_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2462491.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            29228..29446
                     /locus_tag="IWI97_000972"
     CDS             29228..29446
                     /locus_tag="IWI97_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462492.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            29459..29869
                     /locus_tag="IWI97_000973"
     CDS             29459..29869
                     /locus_tag="IWI97_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462493.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            29921..32449
                     /locus_tag="IWI97_000974"
     CDS             29921..32449
                     /locus_tag="IWI97_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBC2462494.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            32465..34675
                     /locus_tag="IWI97_000975"
     CDS             32465..34675
                     /locus_tag="IWI97_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462495.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            34683..35774
                     /locus_tag="IWI97_000976"
     CDS             34683..35774
                     /locus_tag="IWI97_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBC2462496.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            35795..36337
                     /locus_tag="IWI97_000977"
     CDS             35795..36337
                     /locus_tag="IWI97_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462497.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            36532..36789
                     /locus_tag="IWI97_000978"
     CDS             36532..36789
                     /locus_tag="IWI97_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462498.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            36803..37135
                     /locus_tag="IWI97_000979"
     CDS             36803..37135
                     /locus_tag="IWI97_000979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462499.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            37180..37728
                     /locus_tag="IWI97_000980"
     CDS             37180..37728
                     /locus_tag="IWI97_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBC2462500.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT        12843 a         5966 c         8062 g        10965 t
ORIGIN      
        1 ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag aaggagtgaa
       61 gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt tgtccctgaa
      121 tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc attgatatta
      181 agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta tgaatcagca
      241 cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct tgcaaatggt
      301 ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg tatgggaggt
      361 tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag cggacaagaa
      421 cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct gcaagaaaat
      481 cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata tgaaatcatt
      541 gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat gttcgggcca
      601 tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa
      661 agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa
      721 atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc agcggacgca
      781 ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa ggagacccag
      841 tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg acattagacg
      901 ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg attgctaaac
      961 atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa gaaaaaatgt
     1021 atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac atggatatta
     1081 tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg gagagttatt
     1141 tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct gtggatcatg
     1201 cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt gattataccg
     1261 gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt gcacaaccag
     1321 cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag gttattttgc
     1381 cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct tatgaggaac
     1441 ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta ggacgagtag
     1501 gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa gaagcgttcc
     1561 aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg
     1621 cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa agagcagatg
     1681 tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc gctgggttga
     1741 ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt atcgagaaat
     1801 ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct gaaacaaata
     1861 caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc ttttgccacg
     1921 ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa cgactccatc
     1981 gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag agttaggctt
     2041 agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac caagcaatgt
     2101 tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag attttaatgc
     2161 agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga tcgtactaga
     2221 tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa attatgaagg
     2281 ccacacattg atcacaacag atggcagtac attactggga gcagatgaca aatcagggat
     2341 tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc ctcacggtac
     2401 catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata aattcgatgt
     2461 tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag gtgagttgca
     2521 gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa atgtacatcc
     2581 agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt ttcataatca
     2641 gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct tccatttgat
     2701 ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg atcatgatcg
     2761 tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt tgaatcagcg
     2821 atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca tgaaagaaat
     2881 cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat
     2941 ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct cttacatggg
     3001 tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat tcgaatttgt
     3061 ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag aattgaacac
     3121 aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg
     3181 tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc tcttttttga
     3241 agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa aagttcaggg
     3301 aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga aaagcagcca
     3361 gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga attagtcaag
     3421 aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg cacgattcat
     3481 catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt acttccaggt
     3541 ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga agaagctgct
     3601 gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc
     3661 attatctcta cactgacaat cggaatctta gcagatacag cagtttggat cagtcaagaa
     3721 gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt attgctgaag
     3781 aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg aaaatcccaa
     3841 ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga cgactcagcg
     3901 accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat tttccgattt
     3961 attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg cattacttat
     4021 ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac tcgaacggat
     4081 ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg attaacagtt
     4141 tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag ttggaagaag
     4201 agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg tgaattttta
     4261 cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct ggcgtgttcc
     4321 tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat tgatctctcg
     4381 aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt
     4441 acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg tcgtctaaag
     4501 gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt gccattattt
     4561 attatttaat caatcaatca tttgcagata tctttgccga attgctgtct accagtgttg
     4621 ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa gggcgctcaa
     4681 tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg ttcttgacct
     4741 ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg atctcggaga
     4801 tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg acagcactcc
     4861 atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg gtcatccaat
     4921 tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct ggtatccttt
     4981 tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat gttcaaatat
     5041 tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa tgggtggata
     5101 catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc caagacagga
     5161 ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg tatgtacttc
     5221 cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc atttcgttta
     5281 tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt ggttcctttg
     5341 aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca gtttcttcgc
     5401 ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt gtgtatgtga
     5461 ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa gagaataaag
     5521 aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa gaaaaacgtt
     5581 atggataaga taattttacg tttgtttggc tcttttttta tatacataca atcaattttg
     5641 tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac
     5701 caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa gtgatcttac
     5761 ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac gcttcaagaa
     5821 gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat cgacattgcc
     5881 catgtatgga aaatcttttt acaatctaat cattttggtc gaaatttcta tacggatgcc
     5941 ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga gtatccagtt
     6001 gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt tcgattattg
     6061 aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt
     6121 attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa taaaaacgga
     6181 ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga aagagtgacc
     6241 tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga tttagtacag
     6301 caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc
     6361 atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga acccggtgta
     6421 gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa
     6481 aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc tggagcaaaa
     6541 ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat
     6601 ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg aaaaacagaa
     6661 ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga attacatttg
     6721 atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa agcgttggaa
     6781 cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat cagtattctg
     6841 cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga caacgcatta
     6901 gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc ggacaaagca
     6961 atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc tatgccaacg
     7021 gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa
     7081 aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa
     7141 cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat
     7201 tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga agatgcagaa
     7261 aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa
     7321 aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat ggttcaagag
     7381 tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat ccccgtaacg
     7441 aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact tcataaacga
     7501 gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg ttcacgggct
     7561 ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc aactggtgtc
     7621 ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga agatcatatg
     7681 gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct agtcggagct
     7741 cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat
     7801 ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc
     7861 ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt agtagatttc
     7921 aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct ggaaggcgtg
     7981 actgctgatg gaacgattcc agaagcagtg gctgaacaag tcaacacgct attacgtgga
     8041 aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac accattgagc
     8101 ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca acgtctggaa
     8161 catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct
     8221 tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga agtagaaaca
     8281 ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt aacgattact
     8341 ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc
     8401 attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct ccttttttca
     8461 aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc
     8521 ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt tataactttc
     8581 aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta aggcagcacc
     8641 tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc cgacaaacag
     8701 tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt cgcataagaa
     8761 ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga ataagaggaa
     8821 caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa tcaatccata
     8881 gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc aaaaagagat
     8941 gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga aaaaccagta
     9001 aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga cttgtggcat
     9061 gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt gagaagtcga
     9121 atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac caaaagcaag
     9181 taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca tctaaaaatc
     9241 tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc aatttgatca
     9301 aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct tgtagtgcag
     9361 cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag ccgatcactg
     9421 ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg atcgtcagcc
     9481 catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca gcaagagcaa
     9541 tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct tgaggaagat
     9601 agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg ttatataaca
     9661 aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca tcatcctttt
     9721 tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc tagtgacttc
     9781 agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc acggcgataa
     9841 ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc tataggttca
     9901 tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc attattccaa
     9961 ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac aaacggaaac
    10021 cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc gatctgctct
    10081 ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat atctccatgt
    10141 acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt tcgagcttct
    10201 tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca gtagaacaga
    10261 acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc atcttcattt
    10321 tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc atggttttgt
    10381 tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa tcgctcaacc
    10441 ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa ggaaagttta
    10501 tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg atacaaataa
    10561 gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag atcttgcatc
    10621 aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc cattttataa
    10681 cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat cagtttattt
    10741 tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga acggataaaa
    10801 gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg gaacacatag
    10861 gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa ttcaagattt
    10921 tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat aacagtatag
    10981 caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta aattactgct
    11041 tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta tggatataaa
    11101 gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc atatcgacca
    11161 tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga cgtctcgcga
    11221 aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa tcacgattaa
    11281 attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata tctttcactt
    11341 gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg
    11401 tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa cattggccaa
    11461 tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata aaatcgacct
    11521 acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc
    11581 tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata ttctcgagca
    11641 gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa aagctttgat
    11701 ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga tcacggacgg
    11761 tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat ttgatgttac
    11821 agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg tcggtgatgt
    11881 cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg atacagtaac
    11941 gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga atccgatggt
    12001 ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg aagctttaga
    12061 aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc aagcattagg
    12121 ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc aagaacgctt
    12181 ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt accatgttaa
    12241 taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg aacctgtaac
    12301 gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa atgattttgt
    12361 cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga tggattattt
    12421 agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg tctttgactt
    12481 tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg aaatgtctgg
    12541 ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa aagtcgatgc
    12601 attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa
    12661 actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag ctattggaca
    12721 aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta
    12781 tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag ggaagaagcg
    12841 gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat
    12901 ggatgagcaa gatagtgtga aataacttat aatcaatcga aaaacctatt aaataaacct
    12961 ttttgagtag atgcaacact gaggattatc tgtataaaag attttaaact gtcatttggt
    13021 tatcacggcg atactaaaat taacaaaatt ggaatatttt tcttgaagat ttttagctgt
    13081 cagtatatga tgaaagcatc tcataggagg tgcttttttc atggttgata attcaagtgg
    13141 aagggttctt aaatctctaa gaaaagagaa aaaactatct caaaaaaagt tagctgattt
    13201 agcaggtatt tcacaaagta ctttagttaa atatgaaaaa gggtcaagaa aaatccctaa
    13261 agacgtagat aatactctaa gcaagattct gaatatagaa actctaataa aagatgagga
    13321 agataagatt gagattctca tcggtaaact aattgcatac agagatatga ataagttact
    13381 taacaaagag ttagctgata atataggaat tagtgaagta ttactcagtt atgtgttaaa
    13441 tagaaagaga aatccgtcaa aagaaatgca aaaaaaaatc gatatttttc tattaagtaa
    13501 tgaaaaagaa attttgaagg agattaatcg tgatagtgag atatttagtt tatcaaaaga
    13561 cgataaaatt gttatgggaa aaagaatacg agaagtccga aagaaccggg aagagacgct
    13621 tgaaaaattt ggtaaaaact ttaccatcta tacaggcaaa aatgttataa gtagatggga
    13681 aaaaggtatt aatattccag atattgaaaa actaatgaat attgcctatc ttggtaaggt
    13741 cactgttcct taccttatgt acggagaaga ttataagaat atacttccaa aagatgaaag
    13801 agttagcgat tttaaaaaga taaattcttt tagtatggga ttaaggatga ggaaaattag
    13861 gaaagattat tatctagaaa gggaggagtt tggaaagctt ttttctcctt caatttcaaa
    13921 atggtccata gataggtatg agaatggtag agatataccc aatactaatc gaattatcca
    13981 atatgcttat ataggaaatc tttctttaga atttttgatt tacgggatat aaaaatagtt
    14041 tacgtcgata cttattaagt tatcgacgtt ttttattata gaaaaaattt ataaaaggag
    14101 gtttcttgat atgataagaa acaaaaaggg tttgttgaaa gttgctttac tggcagcttt
    14161 tttctaagta actttttgac aagctcaatg gcaacacaag cggaagaagt aatacaagaa
    14221 ctaactgtta tgaataacag ctagataaaa tgggctatgt atgttatcca acaatttagt
    14281 aatcactaga agcctagcga aagttcgctt gataagttat agatgagtat tgatttgtag
    14341 tgtttccaaa tcaaaagggt acagagccac atgaaatatg ttgtcctctg cagcgtcagg
    14401 atgggaatcc ataccatgga gattcggaga tctgtgaggg aatacttacg tggttaatat
    14461 gtcagttgtg ttggacgtag taatggtcat agtgggtaga ttgactgaat tcttatatga
    14521 gaaaatgaaa ccaacaagaa gatagggtta tttatacaga attggggatc ttttctggaa
    14581 aagtaatcgg ataatgcgag attcgtacgg tcttttttaa atttctctaa aaaaaacggc
    14641 atcgaatggg ttgggtgatt gataagcaat gaatcataga ctgatataga ataaaaaatg
    14701 attctattta tctttttaca tctatcgttt tttcaagtag ccgcaaatta tatcagcatt
    14761 caacaatttt tgtatacaag ttctagtaaa aggatctgaa gaaaaagttg gttgttgtga
    14821 agataaaaaa agaatcaggt tttactgagt ttttcttcaa aatttgtgtt attaggttag
    14881 gacaaagata agaagaagta tgaagattta aaaaaggaat gaggaaaaga tagtaatttc
    14941 cgattattgg agttttttta tttataaaga agaagcgaat aagttgtgat tcctagattt
    15001 cgcgcgtgat taattaatga gcaaaaaatg cggaaagttt tagtgtttaa tacacgagga
    15061 caaagtttct taggtattga aggcacacgt gtaacggttg atgggtggtg tgattgtttc
    15121 gaggttggaa aacatgttga acttatggca tttactgagc taaaagataa gaacaacgtg
    15181 gaatttttcg agagtgatat tggttgggac gatcatgaag aagtttatgg aaaatttatt
    15241 ttgcggaagg gaaattctta tacgaatggg gaagcataag tagcgatttg ttagagatca
    15301 ttgataggat tgaaattgtc gggaatattt atgagaatct agagttattt gaggtgatag
    15361 aatgagtgat ggttttgaac aagtatcaat aattaaaaat caagttcggg agattcttag
    15421 aaaaaaaggc tatttggtag atagttattt tgaaggagat tacgaaacat gggtaggtgt
    15481 ctatgctcgt ccagagaata aacctaccta tttagatcct acgacgtcag aagatgccta
    15541 tttacaaaat aggtatcgag tagatggttt taagcaagac tttgcggagt ggtttgaatg
    15601 ggaaattgaa aacggagaag taaaagaaga ataagagtac ttgttgtatc aaaataagcc
    15661 agtagaacaa ccaaaccagt agctagtaat gacaataata ctgaggaatc ggatcattat
    15721 ggtatgattg atatttctct tccagaagat cttaagccag atcctgtaac aattggcaat
    15781 agtgtcatga tttttgattc attagatgaa gctatgtcat atggttggaa agaaattaag
    15841 gatgaaggta gtaattggta tggcatgaga tttggtgatg gtgaagttgc caaaaaagct
    15901 ggtattccaa ttgggaaatg gacagttgat ttccacgaag caggttcttt ttttgttctg
    15961 taattatagt gggagggtaa aatcattgat gaaatgtgtt cagataagag cgccagataa
    16021 aaatagaaga gacagttcac gtcgttatca cttttggtaa cggcgttttg ttttattata
    16081 taaaattctc aggaaaagga ggaattcttg tgtatgcaaa aacgaaaatg aattggttga
    16141 aagcactcgc attggtggct ttttctcaat tctgtttctt ggtgggaact gctcaagcac
    16201 atgcagaaga aacgacggct atacaaccta cgatggaagc cattgcccaa gtcgcttctg
    16261 aagtgacaga agtccaacca gcagaagccg gggtcccagc tggtgaagcc actgttgaac
    16321 aggcccctgt gacagaagcg ccaacggtca attccaaaac gactctcgat cagacgattg
    16381 gggaaacaca acacccaggg aatagtcctg gtacttcaca accagttgaa gaaactgcct
    16441 caattacaac ccctgcttca gaaacgtcca ctctagaagg agaaaaagct gccgaagcaa
    16501 cgccttctgt gcagccagag tcttctgaga aacaagcgga agaaaagaaa cgaatagaag
    16561 aaacagtgga acaaccagcg gctactgctg acaaagaagg aaaaaatgag gaaaacgttg
    16621 aaaaaacaat ctcttcaacc gcagctagtc aaaatcaacc aacgaaacca aacaaagaat
    16681 ctacggctag tattgaaaat caaaagaaaa aagagcagaa cattccaaaa acaggtacac
    16741 ctacactgtt agtaacagta ccaaccaatc gttcggttcg tgttcgccgt gcaaccaatg
    16801 cagctgtttt aggtggtcgg aaaattgatt ggggcgaaaa tattggtgtt acttctagtc
    16861 cgaacaatct aaacttcact ttaaattata tggttcgttt tgagattaat ggacaagtgg
    16921 cgttttgtat tgagcctggt gtctcagccg cagaaggtca agtttataca gctactgctt
    16981 tgaaaaacta tttgaaaaat tcaaatgtac ggaaaaaaac aagcttgatc tcttatttag
    17041 gatatgtcac aaataaagac aaatccgatg aacaatacat tgcggcacaa tttatgattt
    17101 gggaagcttt gggcacaaaa atcccaatca aaaatatccg tgttgattat gatcgtcgaa
    17161 aagcagaaat tcaacgtttg attaataact ttggtgaaaa agcaactttt aataatcaaa
    17221 cacatacggt aaaaattggc gaaacgttga aagtaaagaa taaaaatgat tttttgaaat
    17281 atgtgaaaga cattcaaaaa ccaaaaggtg tcaatgtatc tattcacggc aatgaagtat
    17341 ggattacagt cacaaaagag gcaccggata aagcaaaaat tcaattgaat aaaattaaag
    17401 ttcaaggtgc gccattagcc tatcgtcgtc caggagctca aacaattggt gtgttacatc
    17461 caacagaacc taatgcgtct gttattaatt tacaaattct aaaacaagga aacattcgtg
    17521 tgtttaaaga agatgcggaa actggtgtga aagcacaagg tgcagcttct ttagaaaatg
    17581 cggtctatgg tttgtatcaa gcagatggga aaaagattaa agaaattacc ttgaaaaaaa
    17641 ttaacggcaa agtacaggca gaaattaaag atcttgatcc tggcaaatat gttttaaaag
    17701 aaatcaaagc gccaaaaggt tatgtgcttt ctgatgaagc aattgaaatt gttgttgaac
    17761 ctggaaaaac tgtcaatgcg accgcaaaag atcaagtgat caaaggaacc attgatttag
    17821 tgaaagttgc taataaagat ttagtcgatt caacgaatcc agataacaaa ccaaaattag
    17881 ctggcattga aatcactttg acgtcaaaaa caactggcaa agtggtgaaa accgttgtga
    17941 cagacaatga tggttatgct acttttggta aaaataccgt tgtctttgat acgtacattc
    18001 tttctgaaac caaaggaaaa gaagggtata aactatttga accacttgaa gtcacaattt
    18061 ctgaaaatgg gcaaactttc cattatgttt tagaagataa agtaattgaa caacgtctta
    18121 aagtggtgaa agtggatcaa gaaacaggaa aagtgattcc acttgctaat acagaattca
    18181 agatttttga tactttagcc aatcgttatg tcacaatgga gattccaaat gatacagaaa
    18241 caacggatat ctttaaaacc aatgaaaaag gctttttcct aactaacggt acacttactt
    18301 atggaaaaga tcgctatcgt ttggaagaaa tcaaagcccc cgtaaattac gttttgaatc
    18361 aaaacccatt agtgttctcg attaccaatg atacagaacc gatcaaagtg atccattttg
    18421 cgaaccgtca agcacggaaa agtgtaaaat tattcaagta tgaagaatgg aacaagaaga
    18481 aaacgccgct cgcaggtgtg aactttacct tgtataacgg taaaaatcaa gcaattggtg
    18541 aatataccac tgatcaaaac ggggaaattt tagtgaaagg tctagttgcc gatgactatt
    18601 acttcttgga aaatgcccca ttagaaagtt tccaaccaaa tacagataaa caagcattca
    18661 cggttgcatt caaacaaaat gcgccagatg atggggaaac gttagtggtt tctgtacgta
    18721 attacttgat tccaccaaaa ctagaaacac aagcgtcaaa taaagccgat ggtgaaaaag
    18781 tggttgatcc attagaaacg atcgtcatta aagacagtgt ttcttatacc aacttgtttg
    18841 tcggaaaaga atacaccatt tcaggagtat tgatgaacaa ggcaacgaat gcgccaattt
    18901 tacaagatgg caaagaaatc acaagtacct tggcctttat tgcagacaaa gtcaatggca
    18961 caaaagacct tgaatttgtg gttaatgcta gtgttctgaa aggtcaaacg atcgttgttt
    19021 ttgaaaaact tttccgtgat ggcattgaag ttgcagcaca tgcggatatc aacgatgaag
    19081 cccaaagtgt gaaagtgaat aatccaaccg ttcataccac ggctgtcttt gaagacggat
    19141 tgacagtcgc agacccaaca ggattgttta ccttaaacga taccgttgaa attaccgaga
    19201 ttcttccagg aaaagcatac ttggtcaaag ggatcttaat ggataaagca accggtgaac
    19261 ccttgcttgt gcatggaaat acggtagaaa cgagtgttgg ctttattgcc aaagcagaaa
    19321 atgaaacggt gatcgtacca tttacctttg atttgacagg tataaatggt cgtgaaatcg
    19381 tcgtgtttga aagtcttttt agaaatgatg aagaactagc aaaccataaa gacatcaacg
    19441 acaaagggca aacggtacga gtaactaacc caagtgtacg tacaacggct acacataaac
    19501 ccactggctt gaaagaggtt aatccattaa cgaaagtcac cattacagac gctgtgaaat
    19561 accacgattt ggtcgttgga aaaacataca cagtcaacgg tgttctaatg gacaaagtaa
    19621 cggaaaaacc agtgctagta gatggaaaag aagtcacaag taccttaacg tttgtggcag
    19681 aaacaacgga tggtacagtc aatctagact ttattctaga cgcacgtgcg ttgcgaggca
    19741 aagaagtcgt ggtctttgaa gatctgttta gagaaggcgt attattggct tctcacgcag
    19801 atattaccga caatgaccaa accatcaaaa tcagtaatcc aaccattacg acgaaagcga
    19861 cgaataaaaa tggcggaaaa gagatcttgg ccttgccaaa tcaaactgtg cttgaatggg
    19921 tcaaagtaga cggtcttgcg attggtcaaa tctatcgctt aattgttcaa ggctacttaa
    19981 caccagacgg cacaccattt gaaggcactc atgcggaaaa agtctttgcg gccgataaag
    20041 aaatgatgga attcttattt gaattcttag tggacggtcg caatcttgca ggaaaaggct
    20101 tgagttttgc ggaatggctc caagcgaaaa cggaagatga gaaagaaacg tttgaagaag
    20161 tggcgaaaca caatgtagat ctaacaaaca aagatcaaac ggttcaaatt gtggaagcgc
    20221 caaaaccacc gcaaccagtg aaaccagaac aacgggttaa accagctact gttgtaccaa
    20281 tgttgccaaa aacaggtgaa caaacaagta gtatacttat ggttcttgga ttagtagtat
    20341 tagtcattgc tttaggtata acactttata aagtacgtaa agccaaagat tagtcatgag
    20401 gaacaagcgg ttaggatttt taaccgcttg tttgattgta atgtagaaat atttagaatt
    20461 tattttggtt tttaagagga ggaaaaactt tatggcagaa aaagcattgc gtatgaagaa
    20521 agagttttta ggtcaagtat ttttattggg ttcacgggaa cctaatccac caaaggaaat
    20581 taatggtgtg tctgggcgat atgttcaatt acaagcagag tgttcgcgag aagaacagcc
    20641 tgcttttttt gtttatctca cagctgaaga ggataaaaga ttacgattac cagaaaaaga
    20701 ggtgcgtatt tcatttgaag aagcttattt tgtttttcgt aaagaacgag tgaatagtcg
    20761 tgaaacaaat gatatgggac aaacagagcg tcgaacaact ggctatcaag atgttggtgt
    20821 ctttttaact ggtgtaaaaa ttggggaggt tttgtaagat gggattaaaa tttccaaata
    20881 attatgacat tacagaagca ttagatgaaa cttgtttagg agccgtacgc tttggcgcat
    20941 tagaagagtc tgatcgtgta tttgataatg aaacaggcga ttatacggga gaaattcgat
    21001 cacagcgagc acaattagtt tttggaaatc cagatgaacc aaagattgca cgacgagtta
    21061 aatttccagc agatgtagat ttgactggtt ttaaacgacg tgatcctgtg aaattaattg
    21121 atccagaata tcatgtggta tatattccag cacaagatgg caatcgtgaa tattcagatt
    21181 tagtgattta tgcgaaagga ttagagaaag tagaagctcc aacaaataat cctaatatga
    21241 aacaatcaca agaaaaaggt ttagatcaaa aacaaccaag tgttgattct aaggaaaaag
    21301 agaaaaaata aggttttttt gaaagaatag gaaggagtct tcctattctt tttatctaag
    21361 atagaaggaa ggggagattt tgaattcaga taaaaccaat caaatacttt gagagattgt
    21421 gcttgttatc aaaatatcca acaattgatt gagtattttg gagacaatcc aacgattttt
    21481 gtacaaacaa atgcatatat ttgtgcggtt attgggcagt gtatgttttt acgtgatctt
    21541 gttcgttttt cattaccgta ccagtggaaa caagatcatc aggaagtgat ttggattcgt
    21601 atagtacgaa tatgtaatat gtgtgcacac ccttataaaa atcgattaaa tctttttcga
    21661 ttatgttttt ttactaaatg tgatattcct gtattagttg ctttttgtca aaagttttta
    21721 gcacaggaaa agtaaaaaca gaaaggagga atgtgacaat tgaaagctca ttttttcaag
    21781 tatcgtggtc ggcgaattcg ctatttcaat cgttatttat ggttttgcta ttatggtttc
    21841 tttttgtttc catttttgat tctgttaggt tggtttagtt ggaaagtatt ttatccacgt
    21901 ttaagtacgt tttcgaattg gcaaatgtat cttattcctg gaataagtat tgtgttgtat
    21961 ttgttgctgg ttagtgggtt aattttaggg ttgatgcatt ggtcgcgact aaaagaagga
    22021 tattttgcct ctgtttattg gcgtcagtta cttgttcgaa tgttaattga caatcggttt
    22081 gtttatacaa agaaacaagt tagtgagaaa caaacaaggg aaaaaatgag attaccaaaa
    22141 gtttattttt atcaaaagaa acaagaatta gtggtttctt ttcctttaga tggtgggcgt
    22201 tttcacgaac gatttttaaa aatgggtcat ttgttatctg aagtattttt gcgtgattta
    22261 attcgtgaag aacgagaaaa agcccgaatc aattatgtat tcctttcaga ttctggtcgg
    22321 attcctattg atcaatgtat agcagaaaat ggacgtattc atttaatgaa aactttggat
    22381 tgggtatatg atcaatcacc aaatatgttg attgctggtg caatcggtgg tgggaaaacc
    22441 tatttattgt attctttaat tcaagcttgt ttaagtgttg ggacagtaga tatttgcgat
    22501 ggaaaagcgg cagatttagc agcattaggt gacgtcgatg tattcaaagg acatgtgttt
    22561 tacaaaataa atgaagaaat gattatttgt ttacgaaatg cattaaaaga aatgaatcaa
    22621 cggtatgcct atatgaaaac catgaaagat cctaaatatg aaccagggaa aaattatgct
    22681 tattatgggt taccaccgca ttttatcttt tttgatgaat gggcagcttt ttatggttcg
    22741 ttggatatgg aagcgagaaa tagagtcgat aaattgattc agccattagt attaaagggt
    22801 cgtcaagcag ggatgtacat tattttagca atgcaacgac cagatgcaga atattttcca
    22861 agtggtgttc gggataattt aacattccgt atttctgttg gtcgattgtc tcccattggt
    22921 tatttaatga cttttggaga agattttaaa aataagccat tttacaacaa agatcaacct
    22981 ggtcgtggat atgcttttcc aggatttgat gtacctgtag agttttttgc gccatttgtt
    23041 tacaaagatt atcactttat caaagaattt gagaaattta ctgaaatgat tgtctatgat
    23101 tttacagtaa aagaggatag tcaagatcaa acgatgggag atgataatca gttgttacaa
    23161 gaacaactag agcgccaaat gtatttagca aaagatccaa aaggaacaag tttttctgat
    23221 gcgcatattt tgtaatcaac taaggaggtg aagttaaatg gcagagagtt gttattatag
    23281 agtagctaca gcaatacgaa tgattaatcc ttctctttct tccagaacgt tttatgattg
    23341 gttgaatcgt attgaacaag taactgatta ccgctttttg cgcaaagaac gtgtttttac
    23401 aggaaaagtt attaatcaag tattgctaac gaaaaaagat atagaacgat tgactcgatt
    23461 gtatcattat cgagtagatt tagaagagga tttaactttg tcaatttatc gcgttttttc
    23521 tcctgaaaaa tatagtgaaa taacaaaatt agatcattta attttgtagt tattatctat
    23581 cgggagggct ctagacgtag tctggtagcc gcactctgcc tggcggtgcg cattaaggct
    23641 gcgaagcatg ggcctataat gcgcactcgc tagcagcgtg tgcagctcac aaagaggtcg
    23701 tgcgcttgcg cacggcctct ttgtgtaaag ccccccgata tataatatgg ggggcagaaa
    23761 taacataaca catagaaaaa agttttataa acccttttgt atcaagtgat tatctaaggt
    23821 cagcagttgt actacttttc ccaaaaaacg agggggacta gttgatatat cggtgtttct
    23881 aaagggttaa aaaaaatcga ttttgttatt ttataaaagg agcgagaaaa tgacaccgat
    23941 tgagctaaaa agctacagaa agaagttaag tgcaattcat aatataagtg gagataaaaa
    24001 gccgctatca caagcaagat ttgcgcaatt gatgggctgc tcaaaagatt acattagaaa
    24061 gatggaacaa gggaaaagaa aaatcccaga ggattttgaa caacgaatca aggaacgctt
    24121 tcgattgtat atgaaaagcg gaatagatct tcgagcaatg attgattatt tgcgtctttc
    24181 cttttttggg acagactaca aaacagtgat tcaacgtgta ttacgaatgg atctgacaga
    24241 atttgaaaca cgtccaacgg gcttgtatct ttttgactgt ctttctgttc gtgggaatat
    24301 ctgggtgttc tggcattcaa aacgaactgt aacagaaaat gtacttgtgc aattttcagg
    24361 acaaggttgc cgggaatatg aggaagtcat gcgtgaacaa caaacagatt ggcgaacatg
    24421 gctatattcc atttggaaaa atcaaggaaa gttggaagct ggttatacac gtgtgcaatg
    24481 tagccgattc gatattgcgt tagatgaact ttggggattg gaaacggaag aacatttcga
    24541 tctctttgaa ttactagaaa aacaaaaagc aggtttatta gaaatggact ttaagagatt
    24601 ccagaatatt ggcggttgtt ttttagatga tggctatttt cgaagtgaag gattatcttt
    24661 gtattttgga tcgcgaaaga gtcctctctt ttttaatttt tatgagaaac gttttgaaat
    24721 tgcgaaccga gaaaaaattt cagaattaga agcattaacg aaatatggta tttataatcg
    24781 atatgagtta cggttagcca atgagaaagc aacacaagca gtagaagcat ttattttagg
    24841 tgattcaccg aaacgtttag gagagattgg cgttggttta attaatgcaa aacttattgt
    24901 ttgggataga ctggaaagtg atgcacggat tgttgataaa cgatgggcaa aattatttcg
    24961 ttcgatggaa gaattggatt ttacagtaaa gccagaaaaa ttctcattag atcgttcaga
    25021 acgctggttt ttgaaacaag tagctccgac attaaagttg ttacaagaag cagatcgtat
    25081 tttaggaaga gactttgtac aagaagggat tgataatgcc gaactaaatg acgagcaaca
    25141 aaactatcta gaatggatgg gaacgtcagg agggaaaatt gcatgaatgc atatacgaca
    25201 acagaatggc tgcgcctgtt ggatttaaag gctgcaatcg aagcattaaa tgaaaagatg
    25261 gttgatttgt cttattttcg ttttcgagtt ccttatattg aacaagcggt aaaagctgga
    25321 cgttatcagg aaaaagagaa ctggcaagaa attgcacgct tattagaagt tcgtaaggga
    25381 tacgaacaag aactcgaaga gttggaattt agccgaagga aaggaaggct agaattcatt
    25441 cgcttttatc gattttcatt gccaatacct gcgattttag cggttaaaaa aggctgcgat
    25501 aaaatgaaga tatatgaaaa ctgtgtggca gcacttagca gcgaaaaacc tcttatggaa
    25561 gaaatttcat tggtgactgt cacttgggaa atgagtcgcc agccaacaga agaacagaca
    25621 tatctttctt tagaagagat tgagatagaa ttagaggaaa ttggtcgcta tgccacttgt
    25681 tctacctatt gtggatcggt cattagtatt gctggtgtga ttgtctaaat gaaaaaaatt
    25741 atgatttatg ttggtgcgta ttggtcacgt gatccaacag tattagaaaa tccagaggcg
    25801 gtacactatt gcttgcgcca attattctat ctgtataaag aacgattaga atcgttgatt
    25861 cgtcagttac catatactga cagacgttta gatgaacttc ttctaaggta tccagcaatg
    25921 tataaacgac gaaaaaatag attgttacca gaagagtatc ccattgaaaa acatgaactt
    25981 gaagggcgat ttgtggctta tttctatgat gatgttcgta tgcgcttagt agaacaacga
    26041 atggaaatag aaaatgatcg ctactatttt atcaattatt gtaagaagcg taagtatcaa
    26101 gtaactgatg atttttatga ttgtatttta caagatgggg aagtgattct atccaaaatt
    26161 gctccaagtt ttcgtgaact tgtgccgatt gattttttga aaaaatgtca tttgcggatt
    26221 cttccgtaaa aaagagctat aaaaggaaag aaaagtagaa gccaaatcgg tttctgcttt
    26281 tttatttttt ttaggaagga gaagatggta tgtcactgga atttttagga gagccaattg
    26341 aaaaggaaga gttcgtttcg cattatatgg atttgtttat tcaggtgata aaaaactttg
    26401 tgcgattgat gaattttatc cacgcgaaca agactatcta gccgctgaac gaataaaaat
    26461 cagtttatta tgggaaaaat tcacagatga aaataaccga ccgccagatt atcgctattt
    26521 atcagattgt ttaacgaata atgtgctttc taaagaatac ctatttcaca aacgtgagaa
    26581 aaatcaaatg tcggttgaac atttttgtga ttgttatatt gcattattgc gtagtcaaca
    26641 gttattaaca gtagaacgat atggtgcaga gtttggttat gtcatgcaag ctgagcgtca
    26701 tagagctaca agatgctatg aaaaagaaga tcaagtgatt gatggctatg aagaattatg
    26761 tattcgaaac aatcgctatt tgcaacaacg tttatatgaa caattgatga tggatttttc
    26821 aaatctctat agagggtatg aatgttcgat gattcaagaa aggtagaaga aaagggggaa
    26881 tttttatgtg gcgattatta tttttggtgg tatgtggtat ggcattgttc tatgtgtatc
    26941 gtgtctatct ctatccacga atggaacgat ggacaagttg gtatagagat cctttaaaac
    27001 aacgaaaagg attgaagcgg tatcgttatg aattacttgt tgaattttcg gatgggcgac
    27061 aagcacagta ttattttttt gcaaactatc caaaatacaa caccagtgaa ctattgattc
    27121 aattattcaa aaataatcca tatgttgggt tagaagaaaa cggacgggag ttcttttatc
    27181 gttcagataa tattagtgcg gcgtctatga acaaaacacg aattgcataa aaaattggag
    27241 gaataatcat gaaaaaaaca acattagcag tcgtaacaac actaggaatt tcattatttt
    27301 cgtttcaatt agggagttca ttggtttatg cagatgaaac tgtagaaaca cctaagtcag
    27361 aaattgtaga aacattgcca ggggaaggat taccagatgt tgagacagag ctaaatggag
    27421 gaacaacgga aaaaccagca gaacctgaac aattgcagtc agagaaacct gaaaaagagc
    27481 cagtgacacc agaaccagag tttcctgaat caactttacc gactgattcg atcgaagaag
    27541 aaggtgtgca tagttcaaca acaacggatt caagtatagt cacaactccg gaacaatcag
    27601 gaacaaccag ttcttctgaa aagaatactg attcgtcaag tacggaacag ccaacgatgt
    27661 cgtctaccag tgaagaacaa aaagaagaac cgaaacaacc tgttgaaaaa tcagaagcaa
    27721 ttccaaaaga aaataaacct attgttccag caaaagaagt cacagtatcg gtaaccccaa
    27781 gcggtgaaat tacgacgaat acttctcaag gaatgagtgt accgattgtc actagtaatg
    27841 tagaggaatt gacccatatt ccaacaccaa ccaccccatt aaaagtagca ggtgatcaaa
    27901 caattgttgg tgtaaaagat ggtattccat taatccaaga tagtgaaggg aatttggtaa
    27961 aggatttgtc gattcctgta aagaaacttc caagtggcaa tattgaaatc aaaacagcag
    28021 atggaaaaac aaaagtctta ccaaagactg gtgaagaaat acatgttgca ctttctgttc
    28081 taggaggagt gttgacaagt attgctggat ttgttggata taaaagaaag agaaaaggaa
    28141 tagaaaatga gggtaattga atggactatt tttcagaaaa acaagcttat cgttgtcaat
    28201 cgtacgtact agtaaaaaca tactagaagg agtagcttat gaaaaaacga caactacggc
    28261 ggaaagaaaa agtttataca gagaaagtac cgaagcaaaa gaaaatccat tcaaaaaaag
    28321 cagtgaaatg gatttggtgt ttggttttat ttctttgttt ttcagggggc ttggcatttt
    28381 ttcaatccat gaaaacgaag gttcaagtgt cgtttttgaa agatgaaatc caacaattac
    28441 aaaagaaaga agtacaaagt cggtcagaat taacgtacac acctgaaatt gaatccttta
    28501 tgaatcgttt tgttgctttg tatatgaata cattcgataa tcatgaacaa gaacaacaac
    28561 gtcaagaaac attaaaaaaa gagttttatg cacaaaactt tattcaagaa ccaggaagtg
    28621 tttcaggtgt tcgaaaatta actgctgctt cgtttattgg attaaaagaa attgaaacca
    28681 ttccaactgc tagttataaa gtaacttatg atattcgctc gatgaatcca gagagtaaga
    28741 aagaaacaag tgtgacaatc acacaagtac tcaatcttcc cttttctatt tcaaacaagg
    28801 gctgtcgtat tattgcttat ccttatttta cggcactacc aaagaataca gaaaaacaac
    28861 gaatgattac ttatgatgac acattatacg aaacggttga tgcgtcggaa gcgaaagatg
    28921 ttgaaggatt tgtggaagat ttcttagata agtatgcgaa aaacaatgca aaagatatgt
    28981 catacatgat gaaagaacct gaaggactca atcaaacagc taaagttgat caagtagaga
    29041 tgaaagtatt gcgttataaa gaaacgaaaa aaccttttat tgtaaaagca acggtcgtct
    29101 ttcgagatgg tgatacgtca cttttacaaa aagaacaaat gacgattgtc attacgaaaa
    29161 aaacaaatca gtattatgtt gaaaaattaa cacataattg gttggatagg aaaggagaag
    29221 aacggaaatg acactgaagg gaattgtaga ttttatatta acgcaaggtg gctatttttt
    29281 aataatggtg gttgtttatc tgggattgcg aaattttatg aaatcaaaag taggaaatgc
    29341 attaatatct atcgcagcag gtgccgcagt ttatttcttc ttaaatgatc caacacgtgt
    29401 atttgatgcg tttggtaaaa tcattgaaaa attatttatg gattaggagg atagtgaagt
    29461 ggattgggaa ccttatgatt attcgattgt ttatgcggaa ccttatcgcg tacaacagat
    29521 tacaagcaat tggcgtcttc catttccagt tgcattagtc aatgcagcaa cttttttgtt
    29581 ggtcgtaatc tttgaatatt tatttttacg ttcatttatt ggttgggtca tttctttttt
    29641 tccaccagca ggagttgttt tttatatagg actcccctat ttatgtgtgt tagcactggc
    29701 aaaactaaaa cctgatggaa aaaaagtgca tttttattta tgggattggt tttgttatat
    29761 gattcgtatg atgaatggtg attgtgtgta ttataactgg gacaaggtat caaacaatgc
    29821 attggaagaa ttactttttg tgaataagta tgtttattta gaggattaga aaaagggaca
    29881 atcaactggt tgttcctttt ttattgggag gtgaaattca ttggtgcttg taaaagaacc
    29941 agtaaaagca ttcaagaaaa attgtttgtt aaatggagag gattgtatct gggcctacta
    30001 tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg aaataaaaga
    30061 aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt tggtgatgta
    30121 tccacgaaca atgaatttac ggacgaagtt tgacacatta ggaaagggat tttctcccga
    30181 tagtcaagca attggtgcgt attatgcaaa agaaacagtg acattgttgg aacgagaagg
    30241 tcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag caacggtgga
    30301 gagttttcat gatgtaataa atctaatctg gttagattta gcaggaagtt tagcacgctt
    30361 atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc cttatgtaaa
    30421 agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa aagagctgtt
    30481 gtatctcaat cgtctgcaat atattcgagg aatggctcac aaagtagaag aacaaaataa
    30541 ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg aacataaagg
    30601 ccttcttcaa ttagaagatg agttagggag tagttattta gcattgttgc ctattgggga
    30661 aacacctgaa aatatgagaa attgtgatct atttcaattt gctcaggatc tttctttccc
    30721 agcagaattg cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag gtattggttt
    30781 aaaactgaat tggctatcgg aacggttcaa aacagagcat aaggaattgg ttcaaaatga
    30841 tgaagaacct agtgaacgct tattaactgt tcgaggatta gtaaaacatt taaagcaacg
    30901 agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat acggtcaaac
    30961 ggttgaagaa gttcgtcaaa atagtgcaac ggttattcaa caacttcata cgattatcca
    31021 tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat tagggcaacc
    31081 attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg gcgaatggtt
    31141 gttttcagca gtatcagaat taggtatgga atccggctgg tatattggtc ggcaagatac
    31201 attaggggat ggtggttctc gaggagaaaa aacaccatta gaagaattaa tttatgcgtc
    31261 aaatcgtttt gtctttttga atcttctagc cattgcggaa ggaatcaaag gtgcgttgtt
    31321 tgatgcgcct catgtagcag taactggaaa aaccggtaaa gggaagacct ttttagttgg
    31381 cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtgg atcccaaagg
    31441 agaaaagcgc aaatggttta cgaaactcat tcaagacccg tattatcaga aacattaccc
    31501 tttgtttgta gaacatttgc aaaaaatgag ttatgtcacg ttagatgcta caaatccccg
    31561 taattatggt gtattagatc caattgtttg tttatcccga gtagatgcga aacaagtagc
    31621 ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa aaggggcggt
    31681 attaaaggga atccaagaag ttttagttca aaaagaagcc ggacaacaag ttggcttgat
    31741 gcatgtggtc gactatttag aacaaatgga acaaaaagag gttcgagaat atggtgagtt
    31801 tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg gagaaaaccc
    31861 gggattagac tttaatgcca aaacaacgat tttagagatc caagatttga aattaccaaa
    31921 tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt gtttgatgat
    31981 tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga aaacagcgat
    32041 ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta ttattgcagc
    32101 aatggcacgt gtggggcgtt cccagatgaa tcaattagtg ttagatacac aatttattgg
    32161 tgacttaggt tcagataaag aaaaagggaa ttttggtgtc gtctttgctt ttgatgaaga
    32221 cagtgagcga gaaaagattt tgaatcattt acgtttggaa gtaaatgaag cgaacttagc
    32281 tgaaatgagc cacatgatta aaggacagtg ttggatgtta gatccctatg gacgtgtagg
    32341 aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa caacggaaaa
    32401 aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga aatgaggtga
    32461 aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt ttttaacgat
    32521 tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc aggtggatca
    32581 atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt tatctgatag
    32641 tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc taaaaatgat
    32701 gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt ttgtcattga
    32761 gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca ctttttcgga
    32821 tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct tgtatgtgtt
    32881 atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg tgtttattcg
    32941 atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta acgtacctag
    33001 taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag aaggctttat
    33061 tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg gtgttttagt
    33121 gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata aagcggctgt
    33181 tgttgatccg tatatattgt taaattatgg gacaacaaat ttagaagaat tgtatcaagc
    33241 agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat tatctgatat
    33301 tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg cttatcgtgt
    33361 ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg tgccattgct
    33421 taatctaagt attggattac cacttgtagt gattggcatg attcgtttta tctttcaaat
    33481 gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt ttgttccagt
    33541 atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt ttcaaaaatc
    33601 tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag acgatttaat
    33661 tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac tcctatcaat
    33721 tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag ctgcagatca
    33781 aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga aacaactgcc
    33841 tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat tagcagggaa
    33901 tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc aaaagtttca
    33961 acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtggttaatc cctttgaact
    34021 aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt atcatggggt
    34081 agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc tttcaaatgg
    34141 tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac aatcattggc
    34201 acaattgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata aaggtatcgc
    34261 ttttgttggt caatcgattc cgttgccttc tggaaaagta cccgtgccac aattaacagc
    34321 tgctggttct ctttcacaac agattcgcca agtgaaaaat caggcgaaaa atgggagtta
    34381 tccagcaatg cagcagttat tgaaccaata ctctgttcct aattcgtcgg tagccactct
    34441 atcaggaaat cgttctgtgc aacaaccggg gcaacgttcg tctgaaagta aaccgtcgat
    34501 ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagccaa cagagcaatc
    34561 ggaaaaaata gaaaaggatc ctaaaacact gccgattttg gacgcaaaac caggaactga
    34621 ttttacttta ccaaaaggac gaacagtcca aaaaccatta tcgaagaaag gatgaggaag
    34681 aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt tttctattgc
    34741 cattcttttt ttcaggagaa ctttctgagc atggaggaga agctacaggt ctagctgtcg
    34801 cttttaatgg ggaatatacc gaaggattac cgattttttc agagattaaa ggacgtggtc
    34861 caatcacaga tgaacatgcg caagcagcag ttggtgcagc tgtaaagtat cagttattac
    34921 cgagtgtgat tctttctcaa ttaggctatg aatcagctta tggacagtca caatctggaa
    34981 gaaacgatac gaactttttt gggattacct ggtttcctgg ttgtccctac ccacaaggtt
    35041 ctgcacgtgg aattggtggt tctgaaggtg gtttctatat gaagtttccg aacttaaaag
    35101 cttgttatga ctattatggc tttatggtgg cgagccaaag caactttaat caatgtgtgg
    35161 gcaataaaga tcctggaagt tgtttattga ttctaggtcg tggaggctat gcggcagcag
    35221 gtattaccga agggagtccg tattatcgag gagcaatgag tatcataacg tcctataact
    35281 taacggaata tgatactttt gcgatcaagc attggaatag cctaccaaca aaaaaaactt
    35341 cgcttattgc aggaaaaagt aaaggcaatg cgaaggtttt ggatcggatc attggacgat
    35401 ccgtaaataa tggggaatgt tatggtggta cggcatggta tgtatctcaa ttaggtggcc
    35461 cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat tataactgga
    35521 caacctatgg ttggcaagta atcatgaacc caaaaccaag tgatttacaa gcaggagatg
    35581 tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac accggaatta
    35641 taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat gccgagcaag
    35701 gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt agtctggttc
    35761 gtaaggtgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa ttttagtatt
    35821 tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg aaaaacaaaa
    35881 aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa gtggaactct
    35941 tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag cgcaacaaat
    36001 gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat ggagtaattt
    36061 cacagatgtt tataaacgca atcaagctgt gagggactat atgacagatc gttgtattaa
    36121 agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga aactttacca
    36181 ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag aaacaatgaa
    36241 taaggttact caatttgtat tacttcaatt gaaagtagat caagaagctc aaaagattga
    36301 tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa cactcatatt
    36361 tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa ctatgtcatt
    36421 ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg aaaaaatatg
    36481 tcttatgaat aactataaga actattctat tttttaggag atggatacga gatgaaaaaa
    36541 ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt tgtatcgact
    36601 gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa cattgaatgt
    36661 caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag agtaaattgg
    36721 aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca tggctttacg
    36781 ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg aatcattagt
    36841 agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa ttcaaaaact
    36901 aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa aaatttctgt
    36961 agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg gattgaagct
    37021 agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca agtctaaaag
    37081 aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt cataaacaag
    37141 atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat aattaaaata
    37201 tttggtgttt taggtgtttt cttattaggt ttaagtactt ttgttagtac tggaacaagt
    37261 gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa taaagaataa attagtagat
    37321 gaattaactt tttattttga tacagttggt tttttagacg aacatggtgt ataccatata
    37381 aaggattttg gtgctattcg tgaacgaatc agtcaagata acgaacaagg aagagcagca
    37441 agagtctttt atgaacatat tattgttcct agaatggaga aagttgattg gaaacgatat
    37501 gctggatgtg tagccattaa ttctcttcct ggaggaggag tggcttgggc tataggtacg
    37561 cagggagatt ttattgaagc attaaaagct tttaattttg aaaaagctgc ggatttatta
    37621 ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta aagagttagc taaggtaaat
    37681 attgtagcag gaattgcagc agctgctgct agctgtgcat tagagtaaaa gtttatgaaa
    37741 agtttagaac aagagcatat ggcttttaaa caagcaatgt ttaaagaaaa tatctattta
    37801 aaccataact atattcgtgt ttctaaggca tgttca
//
LOCUS       DADXTH010000020        37250 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.020, whole genome shotgun sequence.
ACCESSION   DADXTH010000020 DADXTH010000000
VERSION     DADXTH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.020"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IWI97_000981"
     CDS             299..1960
                     /locus_tag="IWI97_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2462501.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IWI97_000982"
     CDS             complement(2030..2377)
                     /locus_tag="IWI97_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBC2462502.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IWI97_000983"
     CDS             2461..3402
                     /locus_tag="IWI97_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462503.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IWI97_000984"
     CDS             3403..4446
                     /locus_tag="IWI97_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462504.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IWI97_000985"
     CDS             4452..5522
                     /locus_tag="IWI97_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462505.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IWI97_000986"
     CDS             5512..6465
                     /locus_tag="IWI97_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2462506.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IWI97_000987"
     CDS             6477..7487
                     /locus_tag="IWI97_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2462507.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IWI97_000988"
     CDS             complement(7772..8593)
                     /locus_tag="IWI97_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBC2462508.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IWI97_000989"
     CDS             complement(8690..8968)
                     /locus_tag="IWI97_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462509.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IWI97_000990"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IWI97_000990"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBC2462510.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IWI97_000991"
     CDS             complement(11724..12311)
                     /locus_tag="IWI97_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462511.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IWI97_000992"
     CDS             12486..13808
                     /locus_tag="IWI97_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2462512.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IWI97_000993"
     CDS             13963..14739
                     /locus_tag="IWI97_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBC2462513.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IWI97_000994"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IWI97_000994"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBC2462514.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IWI97_000995"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IWI97_000995"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBC2462515.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IWI97_000996"
     CDS             16629..17258
                     /locus_tag="IWI97_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462516.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWI97_000997"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWI97_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBC2462517.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IWI97_000998"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IWI97_000998"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBC2462518.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IWI97_000999"
     CDS             19445..19981
                     /locus_tag="IWI97_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBC2462519.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IWI97_001000"
     CDS             complement(20021..21784)
                     /locus_tag="IWI97_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462520.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IWI97_001001"
     CDS             complement(21787..23502)
                     /locus_tag="IWI97_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462521.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IWI97_001002"
     CDS             complement(24281..24505)
                     /locus_tag="IWI97_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462522.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24696..27473
                     /locus_tag="IWI97_001003"
     CDS             24696..27473
                     /locus_tag="IWI97_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2462523.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27554..27973
                     /locus_tag="IWI97_001004"
     CDS             27554..27973
                     /locus_tag="IWI97_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBC2462524.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="IWI97_001005"
     CDS             27985..28455
                     /locus_tag="IWI97_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2462525.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="IWI97_001006"
     CDS             28472..29251
                     /locus_tag="IWI97_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2462526.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="IWI97_001007"
     CDS             29238..30059
                     /locus_tag="IWI97_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBC2462527.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="IWI97_001008"
     CDS             30076..31125
                     /locus_tag="IWI97_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2462528.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="IWI97_001009"
     CDS             31138..32142
                     /locus_tag="IWI97_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2462529.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="IWI97_001010"
     CDS             32775..33794
                     /locus_tag="IWI97_001010"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBC2462530.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33837..34718
                     /gene="galU"
                     /locus_tag="IWI97_001011"
     CDS             33837..34718
                     /gene="galU"
                     /locus_tag="IWI97_001011"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBC2462531.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35018..35395
                     /locus_tag="IWI97_001012"
     CDS             35018..35395
                     /locus_tag="IWI97_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462532.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36001..36615
                     /locus_tag="IWI97_001013"
     CDS             36001..36615
                     /locus_tag="IWI97_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2462533.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT        12228 a         6504 c         7600 g        10918 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttaggaggaa cagcaagtga
    33841 aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac acgttttttg ccggcaacaa
    33901 aagccatggc aaaagaaatg cttccaatcg tggataaacc aacgatccag tttattgtgg
    33961 aagaagcatt ggattcggga atcgaagaca tcttgatcgt aacgggaaaa gaaaaaagac
    34021 ccattgaaga tcactttgat gcaaatatcg aattggaaaa caatttgcgg gaaaaggaaa
    34081 aaacagaatt actcgcttta gttgaagaaa cgactcaagt caatcttcac ttcatccgtc
    34141 aatctcatcc aagaggactg ggggatgccg tgcttcaagc aaaagcattt ataggaaatg
    34201 aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga tgatatccca ttgactcaac
    34261 aattgatgat ggattatgaa gaaacacaag cttccgctgt tgccgtcatg cgtgtaccag
    34321 aaaatgaaac atctaaatac ggaattattg atcctaaaac agatagcgaa aagggtcgtt
    34381 gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa agcaccaagt aatttagcca
    34441 ttatcggacg ttatctactg actcctaaaa tatttgagat attagaaact caagaaccag
    34501 gagcaggaaa cgagatacag ctaacggatg cacttcagac tcttaaccag acagaggcgg
    34561 tctatgcgag agagtttaaa ggaaaaagat acgacgtagg cgataagtta ggatatatga
    34621 agacaaatat tgaatatgga cttcaacatt cggaaattgg gacttcatta agtgcatata
    34681 ttatccagtt gagtaaaaag ctaagaacag aaagttaact ggaaataagt ggcctgctga
    34741 gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg tcatttgcgc aactgtcgtt
    34801 ttagtttttt gactctagat cagttgagtc gtggaaaacg aaagatagaa aaagactttc
    34861 gtgaatagat tcagaaaata ttatgctaat ggtttaattt ttttttaagt tgtataaaag
    34921 aaaatgatta cataagagaa atgaaagcca agccagaaga ttcatagtag aataaaaagg
    34981 cgaatttttt aattataaaa aaaggtgtgt gttcactatg ggaaaattag tcgctgttgc
    35041 acagtcgctg cttgatatac tcaagtggct gatggaagga agcaattgga tatttgtgtt
    35101 gccactattg cttattagtc tatttatcat gtttcgcatg gtcagaaaaa aagaaacgtt
    35161 tctgattttt atctggtatg cagcatccgt tggggttgct accttgatgg ttgaattggc
    35221 gttaggtagt tgggaagctt gcgtatttgc tttatttttt gctgtactgt atctaatcat
    35281 ttatatccca tatatgaaaa gaaagtctcg gataggcaga aaaggtagac atacaactac
    35341 tttttctagt acagattctc tcgaacaaag tagaagaacg agaagaagac ggtaatattt
    35401 agtaggtata cgcaaaaaaa gataaaataa tggataaact attgagatga gagatttatt
    35461 taattggtgg aaaaaagctt ttttcactta tataaaagaa tcttttttaa aggaatgctt
    35521 gttgtatcgg gataagccat tccttttttt atgttattta tatatatata aatcatacca
    35581 atgttttttt gtattttctt gcttgcactt ggataagaga ttacttacaa taaattcaga
    35641 tagaaacagc tgaataaaaa aaggctccct gacccttagt tggcgctaag aatcagggaa
    35701 tgaccagtca cacacataca ccctgaccga tcattttgcc atagtcaatg cagtgcatcg
    35761 acctaattta ttgtacctaa tctagggtga aaattctacc cctatgtaga tattttccat
    35821 ggtctgcacg ttgttttatt tgctatgtca aggtattggg cgtattcgtt caatgccttt
    35881 tttgtgtttg aaacagcaaa gaccacatgt gttactgtct aactactgtt tttttatgta
    35941 cttccttaga ttaggcagct gtttctattc atgtcaagta tagctaggcg ggagagataa
    36001 atgagtttat gttattttga ctattatcac caattggaag agaaattaag gatagaagag
    36061 ccgctgatgc ttcaagctgg cacttggatc gcgaaacaaa ttaatcaagg tggtagattg
    36121 cagatatatg ctagccgcac actaagtggt gtagcatttg aattttggga tcaaaccccg
    36181 gatcttgtac caagtatatt gattgaaaat ccagccggag gtgtctatga aacgctagaa
    36241 ggtgcaggac aggcaattat tgatcagctt tctgtcaaat cagaagacat ttttcttctt
    36301 ctttcgaatg aaggaagaaa cccggcaatc atcgaactag ctcagtggat caaagaaaat
    36361 ggtcatccat taatcattgt tactggattt gatttgtcac gaagtatcaa atcgcaacat
    36421 agcagtggtt tgcgattatt tgaatttgct gatttggttt tggacaacca cgccaattta
    36481 catgatgcag tcttgacttt gccaaatcta gatccagcga tctgcggaac agcctctctt
    36541 gcaacgatct ttttgctgca gcaagttttg tattttacag caaaacagct agtccaggac
    36601 acaagtggaa aatagttatc cacatgttga caaagttcct taaaaaaata atgttccaca
    36661 taaaaatgga gcgtttgaaa aaaagaaaac gattattaat gaggattaac aacgactact
    36721 ttttttgttt tagttatttt aagatggatc atctttgatt ttaagcttgt tttgtttaaa
    36781 gtttgttatt tttataagtt gtttcacagt tatgagaaat gagttataag aatatgtagt
    36841 gtcagaaaaa taaaaagtag tggaaaaaga gacaagaaag aatccatatg gattctttct
    36901 tgtctcttaa tacacgttga tgaaagagtc ttccccaaaa ctctttacaa agtaattata
    36961 gtacatttgt tatcattatt gcaatgaagt ttacctgata taacaaaatt gtccaacagg
    37021 ataccttttt ctaatagaaa taaaatacac aaaaattgaa ggatagataa tggatattta
    37081 taagtgttta ttattttttt tgcaatgaaa ttcaattttt caatgattgt aaaatgaaaa
    37141 aaacaggacg agattttctc taatctaaaa ataaaaaaag aattttaagg aaaaacagag
    37201 aaataaaata cttaatataa atggaaagag aaaaaaataa tcatacatag
//
LOCUS       DADXTH010000021        36809 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.021, whole genome shotgun sequence.
ACCESSION   DADXTH010000021 DADXTH010000000
VERSION     DADXTH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.021"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            75..521
                     /locus_tag="IWI97_001014"
     CDS             75..521
                     /locus_tag="IWI97_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2462534.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(580..1041)
                     /gene="mscL"
                     /locus_tag="IWI97_001015"
     CDS             complement(580..1041)
                     /gene="mscL"
                     /locus_tag="IWI97_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBC2462535.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            1293..2561
                     /locus_tag="IWI97_001016"
     CDS             1293..2561
                     /locus_tag="IWI97_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBC2462536.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2554..3840
                     /locus_tag="IWI97_001017"
     CDS             2554..3840
                     /locus_tag="IWI97_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBC2462537.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            4579..5502
                     /locus_tag="IWI97_001018"
     CDS             4579..5502
                     /locus_tag="IWI97_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462538.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            5711..6289
                     /gene="pgsA"
                     /locus_tag="IWI97_001019"
     CDS             5711..6289
                     /gene="pgsA"
                     /locus_tag="IWI97_001019"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBC2462539.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            6529..7770
                     /locus_tag="IWI97_001020"
     CDS             6529..7770
                     /locus_tag="IWI97_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBC2462540.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            7879..8928
                     /gene="recA"
                     /locus_tag="IWI97_001021"
     CDS             7879..8928
                     /gene="recA"
                     /locus_tag="IWI97_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBC2462541.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            9339..10895
                     /gene="rny"
                     /locus_tag="IWI97_001022"
     CDS             9339..10895
                     /gene="rny"
                     /locus_tag="IWI97_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBC2462542.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10974..11780)
                     /gene="proC"
                     /locus_tag="IWI97_001023"
     CDS             complement(10974..11780)
                     /gene="proC"
                     /locus_tag="IWI97_001023"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBC2462543.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            11921..14494
                     /gene="mprF"
                     /locus_tag="IWI97_001024"
     CDS             11921..14494
                     /gene="mprF"
                     /locus_tag="IWI97_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBC2462544.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            14636..15499
                     /locus_tag="IWI97_001025"
     CDS             14636..15499
                     /locus_tag="IWI97_001025"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBC2462545.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            15744..16682
                     /locus_tag="IWI97_001026"
     CDS             15744..16682
                     /locus_tag="IWI97_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBC2462546.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            16705..17385
                     /locus_tag="IWI97_001027"
     CDS             16705..17385
                     /locus_tag="IWI97_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBC2462547.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            17358..18182
                     /locus_tag="IWI97_001028"
     CDS             17358..18182
                     /locus_tag="IWI97_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBC2462548.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            18349..19167
                     /gene="purR"
                     /locus_tag="IWI97_001029"
     CDS             18349..19167
                     /gene="purR"
                     /locus_tag="IWI97_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBC2462549.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            19670..21043
                     /gene="glmU"
                     /locus_tag="IWI97_001030"
     CDS             19670..21043
                     /gene="glmU"
                     /locus_tag="IWI97_001030"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBC2462550.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            21137..22108
                     /locus_tag="IWI97_001031"
     CDS             21137..22108
                     /locus_tag="IWI97_001031"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBC2462551.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      22114..22216
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22282..22851
                     /gene="thiT"
                     /locus_tag="IWI97_001032"
     CDS             22282..22851
                     /gene="thiT"
                     /locus_tag="IWI97_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBC2462552.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(22932..23705)
                     /locus_tag="IWI97_001033"
     CDS             complement(22932..23705)
                     /locus_tag="IWI97_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462553.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            23971..25671
                     /locus_tag="IWI97_001034"
     CDS             23971..25671
                     /locus_tag="IWI97_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBC2462554.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            25641..25859
                     /locus_tag="IWI97_001035"
     CDS             25641..25859
                     /locus_tag="IWI97_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBC2462555.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            25879..26769
                     /locus_tag="IWI97_001036"
     CDS             25879..26769
                     /locus_tag="IWI97_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBC2462556.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            26787..27071
                     /locus_tag="IWI97_001037"
     CDS             26787..27071
                     /locus_tag="IWI97_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462557.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            27558..28172
                     /gene="gmk"
                     /locus_tag="IWI97_001038"
     CDS             27558..28172
                     /gene="gmk"
                     /locus_tag="IWI97_001038"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBC2462558.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            28177..28482
                     /locus_tag="IWI97_001039"
     CDS             28177..28482
                     /locus_tag="IWI97_001039"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBC2462559.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            28735..31143
                     /gene="priA"
                     /locus_tag="IWI97_001040"
     CDS             28735..31143
                     /gene="priA"
                     /locus_tag="IWI97_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBC2462560.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            31158..31649
                     /gene="def"
                     /locus_tag="IWI97_001041"
     CDS             31158..31649
                     /gene="def"
                     /locus_tag="IWI97_001041"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBC2462561.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            31642..32580
                     /locus_tag="IWI97_001042"
     CDS             31642..32580
                     /locus_tag="IWI97_001042"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBC2462562.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            32580..33938
                     /gene="rsmB"
                     /locus_tag="IWI97_001043"
     CDS             32580..33938
                     /gene="rsmB"
                     /locus_tag="IWI97_001043"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBC2462563.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            33951..34691
                     /locus_tag="IWI97_001044"
     CDS             33951..34691
                     /locus_tag="IWI97_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBC2462564.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            34688..36757
                     /gene="pknB"
                     /locus_tag="IWI97_001045"
     CDS             34688..36757
                     /gene="pknB"
                     /locus_tag="IWI97_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBC2462565.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
BASE COUNT        12099 a         6503 c         8007 g        10200 t
ORIGIN      
        1 attcgtaagg ttcgtttgct tttttgtgtg cgaatcggta tgctaagaat aaaaaagcag
       61 ggggcgtttg cttagtggaa tatcaggtaa agaaatttga agaattgtca acagcagagt
      121 tttacgcgat cgtcaaagaa cgaatcgcag tttttgttgt ggaacaacag tgtccttatc
      181 aagaagttga tgacattgat ggacaagctt ggcatacgtt ttttcaagat actgatggga
      241 agatccttgc ctacacgcga gtatatgaag aagcggagag tatccatttt ggacgtgtcc
      301 ttgttcatca ggacaaccgt aaagatggtt taggaagaaa gattgttcaa gaaacgatta
      361 acatgatcca gaaaaatttt ccagaaaaac caatcgtgat cggcgcacaa gaatacctac
      421 aagcttttta tgagtcgttt ggattcaagg caatctcaga tgtgtatttg gaagatgata
      481 ttccacatat cgacatgaaa aaggaacaag tagcaagtta agaatctaaa aaaagaaagc
      541 cgacactgaa aatcggcttt ctttttttta gattctttat tatttagaag tatcggaaag
      601 atttgtttgc tctactggat gtgtattgtc ttctgccact tgtttggcaa gaagatcgcg
      661 tatgtcacta agataatctt ctgcagtagg agtttcttcc acttccactt gctcttcttt
      721 ggagcgtaaa ttcttcgcac ggtttgccgc tttgacaact aagaataaga caaaaccagt
      781 gatcagaaat gtaatgatag cactaataac gttaccaaac gcaaatgtga cgccttttgt
      841 cggcgaccag tcaagaattg aaagtgctcc ttctaaatca gaagtacctg tgatcattgc
      901 gacaaaccaa cctattagtg gtgtgatcaa gccttgcacc acttggttta cgatgcttgt
      961 aaacgcacta ccaataacaa cgccgacagc aagatccaag acatctccac gcatgatgaa
     1021 ctctttgaat tctttgatca tgaatgaccc tcccttcttt tatttgtctt tagtatatcg
     1081 aaaaataaaa aataaaaaaa caatatacgc ttttagccgt ctgtttcttt tgataaaaaa
     1141 catctatgct atactataaa gtgtaacatc atttagaaaa aacggcgaat tgcctgaact
     1201 tataaatttc aagcagagga ataagtaaaa aaggcaggtt gacccattaa aagagtaagc
     1261 gggcaatcaa ctgtgaaaag gagcatataa atatggtgta tcaattggca gaaggcgtga
     1321 atcttcatgt cttaccaaca aaacaataca aaacgatccg gatttttatc cgttttactg
     1381 cgcgtctgca acaagaagtc atcacgaaac gttcacttct cagcagcatg ttggaaacaa
     1441 atagtttgaa ttatccggat cagacaaagt taagtgcgca acttgcagag ctttatggcg
     1501 caagctttgg attatcagtt agaaaaaaag gaaatttaca ttggctaaac gcagggatca
     1561 gttttgtgaa tggtgaatat gtcaatgatc caaacctttt ttctcaagcg gtagattttt
     1621 taaaagaggt tttattttat ccaaatatta aaaatcatca gtttgatcag ttgacgtttg
     1681 acttggaaaa gaacaatctg cgtctatatt tggaaagttt gaaggaagat aaacaaactt
     1741 ttgcttccta tgccttgcaa gaactttatt ttgaaaacag cccggaacaa aagatcccag
     1801 ggttgggcgt agtcgaagat ttggacaaga tcactgctag ttcgctagcg tcctactatc
     1861 aagaaatgat ggcaaatgat caaatcgaca tttttgttgt cggcgatgtt gatccggaca
     1921 aagcagctga agcagttggg caattaccat ttgagtcgcg ggaaacagtt tatccagatc
     1981 tgttttatac ccaacctcaa gtgaatatcg tgaaggaacg acaagtacgt gagccaatcg
     2041 ttcaagcaaa gctgaaccta gcttatcaga caaatgtgta ttacgatgaa ccagaacgtt
     2101 ttgcgctaat ggtctttaac ggtttatttg gcggttttcc tcactctaaa cttttcatga
     2161 atgtcagaga gaaagaaagt ctagcttatt atgcctctag tagcgtggat acgttccgag
     2221 gattcatgag tgtccaaacc ggtatcgatg aaaagaaccg aaatcaagta cttcatctga
     2281 ttcacgagca attagaaagt ttgcgaaatg gtgaaataac agagttggaa ctgtctcaga
     2341 caaaagcaat gctgagaaac caatatctgc tttctctaga ctctccgcaa gcggcaatcg
     2401 aagcttcttt tttggacagc tggctgccgg aaacaaaatt atctgatgaa gaatggttga
     2461 aacgtatgga gagcgtaacg atcaaagaga tacaacaagt agctgagcaa atcgaattgc
     2521 aggcaatctt ctttttagca ggaggaaatg caaatgaata aaacagaata tgagcaaatc
     2581 aatgaaacac tttatcatga agtactgcca aacggtttga ccgtgtattt gttgcctaaa
     2641 aatgactatc acaagacata tggtttgttc agtacgaatt atggttcgat cgataacgaa
     2701 tttattccct atggtgaaaa agaaaaggtg aaagttccag atggaattgc ccatttttta
     2761 gaacataagt tgtttgaaaa agaagatggt gatgtctttc aattatttgg gaaacaagga
     2821 gcttccgcca atgctttcac aagttttacg aaaaccagct atctgttttc tacgacggat
     2881 caggtagaaa agaatctgac gactctgatc gattttgtac aagcacctta cttcacagaa
     2941 gaaactgtaa acaaggaaaa aggaatcatc ggtcaggaaa tccaaatgta tgaagacgac
     3001 cctaactggc ggatgttttt tggcatacta aataacttgt atcctacaca cccattgcat
     3061 atcgatattg cgggcacggt tgaaagtatc gacaaaatca cagctcaaga cttgtatacg
     3121 tgctatcgga cgttttatca gccaagcaat atggttttat ttgtagttgg taagatggag
     3181 cctgagaaat tgatgaaact tattcgggaa aaccaagaag caaaaaattt ccctccaaag
     3241 caagaaattg tacgttattt ccctgaaaac acaaaagaaa tcatcaaaca aagtgcattg
     3301 gaagcagcca ttacacgaga taaatttgta cttggtatta aaggattaga cacattgccg
     3361 caagaaggaa cagaactgtt acgctataaa acagcaatca atctgttgtt ccaaatgatt
     3421 ttaggcaata cttctcgaaa ctatctagcg atgtacaatc aaggaattat cgatgacagc
     3481 tttggttttg aattcagctt ggatcgtgaa tttcactttg cggacttcag cggagatacg
     3541 gacgaaccag agaaagcagc agaaaaagtc aaagaaatca ttcttggatt cgctgatgat
     3601 ccggaagtgt cagaaacaaa tctcgatttg ctgaagaaga aaatgcttgg acaatatttc
     3661 caatcattaa attcgatcga atatattgcc aatcaattta cgcaaagttt atttggcgat
     3721 cgaactttat ttgatcttcc tgaaatcatc gattcgatcc aaatgaaaga cgtactggca
     3781 gctggggaag catttataag tgaagaggcc ttcagcgagt tttacatgag accaaaataa
     3841 accagtattt tatgaggaaa agtcttaaat ttcgttttag acttgtagta ggaacctcct
     3901 ccaaaatcag cttttatggt attcttagtt gtgttggaaa aattagtgag aaaataaaac
     3961 aaatcatgct ctcttttaat caacttatat taaaataaaa tcaaacgaag taatcaaaga
     4021 ggttgtgaca gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc
     4081 aaatttttgt gatatttcgg cttattgccg aagaagctac ttctggaaca ccgtgtacca
     4141 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt
     4201 cttaatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
     4261 aaggaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacat acggaaaaat
     4321 atctccttat attttgccgg tttttgagct aataccgagg aacagctgat tgaacaccgt
     4381 ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca agttcaaaaa
     4441 acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc aggagaacaa
     4501 tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca aacataacgt
     4561 ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga tgaacgagtg
     4621 gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa aatatcttta
     4681 gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat cgacaatgat
     4741 gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca atacgctgaa
     4801 gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag caaattgaca
     4861 ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa agcaatgcat
     4921 caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt attaggattg
     4981 gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg tcaagctgat
     5041 ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga ggagtcctct
     5101 tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac aacaaaatca
     5161 agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc catcgcagta
     5221 aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa tcgtgtatgg
     5281 ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc aaatgaaaca
     5341 cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg tgcagcaagc
     5401 aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga aaacaatcag
     5461 agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa attagtttac
     5521 gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg tcgcggtcct
     5581 tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata aattcatttg
     5641 gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag ggaagaaagg
     5701 agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta tgatcccgat
     5761 tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag gagacaccac
     5821 actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta tcacagactg
     5881 gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca agtttgcgga
     5941 cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg accaaggaaa
     6001 ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca caggcttgcg
     6061 tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa aagtcaagac
     6121 ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat tcagcctgat
     6181 cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta tctattcagg
     6241 tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag tatcgtttag
     6301 tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt caaaaaacag
     6361 cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga gaacactgtt
     6421 tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct ctttttaaat
     6481 aaaaaaatat aaaaatacag cgtatactat ataagggagc gggaagtaat gaaagcagaa
     6541 ataatagctg tcggaacaga acttttacta ggacaagtcg tgaatacgaa tgccactttc
     6601 ttgtcagaac agctagctga tctaggtatc gaagtttatt atcaaacagt tgtaggagat
     6661 aatcagcagc gtttagaaga gttgattgct ctagctgaaa caagaagtga actcatactc
     6721 ttatgtggcg gattaggacc gacagaagat gacctgacaa aagaagcaac tgcggctcac
     6781 ctcggtaagt ctctcataca aaatacggaa ggatacaaaa aattattggc atattttgaa
     6841 acaacccatc gaaaaatgac caaaaacaat ctgcaacagt cccagattat cgaaggcggg
     6901 gttcctctac caaatcgtac agggcttgct ttagggactt tttatcaaac agatacacat
     6961 gcgtatatcc ttcttcccgg tccgcctaat gagttgaaac caatgtttgt ggaacaagta
     7021 cgtcctttac tagaggaacg ttttccttct gaagaaaaac taatttctaa agttttgcgc
     7081 ttttatggga ttggtgaatc gcgactggtc actgagctaa aagatctgat tgagacacag
     7141 atcaatccaa cgatcgcacc atatgccaaa ccaaatgaag tgacattacg actgacagtg
     7201 aaaacaaatg atgttcaggc agggaatcaa gctcttttag cattagaaga aaaaatccaa
     7261 gaacgagtag gggaatattt ttatggatac ggcgatgata attcgctagc aaaagtcgta
     7321 gtggaactac tgaaagaaaa caagcagact gtaacagcag cggaaagttt gacagccggc
     7381 gccttccaag ccgcattagg cgacattgct ggtgtatctg aagtatttcc aggcggattc
     7441 gttacctact cgctccaaac aaaagctggt tttctggaga ttgatccgga gctattagca
     7501 gaatatggaa cagtcagcaa agaatgtgtg gagcagatgg ccattcaagc acgtgagata
     7561 gcaagaacag attacagtgt agctttcacc ggagtggctg gaccggacac gttagaaggt
     7621 caatctgcag gaacagtatg gatcgctatt tcatctaaag acggcgtggt ttcggaaaaa
     7681 tatcactttg ccagagatcg tgctgctatc agatataatg cggttatgca aggatttgat
     7741 cttttgagaa aacagctttt aagaaaatga taaatgcgaa tatttgttcg gttttttctt
     7801 gctttttttc actaaaaaag atatgataag gataatgaaa tgttgctctg atttgtaaat
     7861 ataaggagga ttatcgtatt ggcagatgat cgtaaagcag ccttagatgc tgcactgaaa
     7921 aaaattgaaa agagctatgg taaaggctct attatgaaat tgggcgaaaa aatcgaccaa
     7981 caaatctcta caattccaag tggctctctt gcgttagatg tcgctttagg tgtcggcggt
     8041 tacccccgtg gacgtatcat cgaagtatat ggacctgaaa gttcaggtaa aacaacagtt
     8101 gcactacacg ctattgcaga agtacaaaaa aatggcggaa cggccgcttt cattgatgct
     8161 gagcatgcgt tagatccgca atatgcacaa aaattaggtg tgaatatcga tgaactactt
     8221 ctttcacagc ctgacacagg agaacaaggt ctagagatcg ctgatgcttt agtatcaagt
     8281 ggggctgtag atatcgtagt agtcgattca gttgctgctt tagttccacg agcagaaatc
     8341 gacggcgaaa tgggtgactc acatgtcggg ttacaagcac gtttgatgtc tcaagcattg
     8401 cgtaaactct ctggttcgat caacaaaaca aaaacaatcg ctattttcat caaccaaatc
     8461 cgtgaaaaag taggcgtgat gtttgggaat ccagaaatca ctcctggagg acgcgcattg
     8521 aaattctacg caacgatccg tttggaagta cgtcgtgcag aacaattgaa acaaggtaca
     8581 gatattgttg gtaaccgtac aaagatcaaa gtcgtaaaaa acaaagtagc acctcctttc
     8641 aaaatcgctg aagtagatgt catgtatggt ttagggatct cacaagaagg agaacttcta
     8701 gacatggcag tagaaaaaga cattgttgat aaaagcggtg cttggtattc ttataaagaa
     8761 gatcggattg gtcaaggacg ggaaaacgct aaaatctata tggctaatca tcctgaaatg
     8821 atggcagaag tttctgctct agttagagca gcttatggta ttggtgaaga agtcgctgtc
     8881 ccagaagatg aaaaaggaca agaagaattg cctttagtag aagaataaaa attatgataa
     8941 acaaaaacgg ctggtctgaa gagtaggtac tctccttgac ctgccgtttt tttattgaat
     9001 aaagaaatcg tgagttattt aattatacga gtgggtacat aaaataaaaa tatctgttca
     9061 tctacaaaga ataatttaat agaaaagttt ctcaatttaa aggtttacct atatgaaact
     9121 agtgtcattt attttaaaaa ccatcgttta atgaataagt agaaagccgt ttctttgttt
     9181 atttaaagtt gacactacgg ggtacaaaca ttagaataaa gttgtgcatg ttttttgtac
     9241 acatatatgc gaagatgcat attgacgagt ttagaataat ttatcataga agctgtatag
     9301 atgataaaaa tgaaaaagat agtatggagg tggattttat ggtattcaac attctcctcg
     9361 ctatcatcgg tttaattgtc ggtcttggtt tagggtttat ggtcgcaaag tcacgtcacg
     9421 agaaagaaat agctggtgca caatcatcag ctgctggaat catcgatagt gcaaaaaaag
     9481 aagcagaaac tctaaaaaaa gaagcattgt tagaagctaa ggaagaaaat cagaaatacc
     9541 gttctgaagt tgaaagtgag ctgagagaaa gtaagttaga attaaaatct caagagaatc
     9601 gtttgattca acgcgaacaa acgctgaatc gtaaagatga ctcgttagaa aaacgcgaga
     9661 actcacttga aagtaaagaa gaagcactta gttcaaaaca gcaattaatt gaagagcgag
     9721 aaaaagacgt tgaaaaaatg attgaagatc aacaaaaaga actagaaaaa attgctggtt
     9781 tatcaaaaga ggacgcacga gatgtcatca tgaaatcgac tgaagaagag ctgaatcatg
     9841 agttaactct aatggtaaaa gaatctgaac agcgtgccaa agaagaagca gatcgcaaag
     9901 cgaaaaactt attatcatta gcgattcaac gctgcgcagc agatcaagtt tcagaaacga
     9961 ctgtttcagt cgttaccctt ccgaatgatg aaatgaaagg tcgtatcatt ggacgagaag
    10021 gtcgtaatat tcgaacgctt gagacgctga ctggtatcga tttaatcatc gatgatacac
    10081 cggaagcagt tgttctttcc ggattcgatc caatccgaag agaaatcgca cggatgacct
    10141 tggaaaaact gatacaagat ggacgaatcc atccagcgcg tatcgaagaa atggtcgaga
    10201 aatctcgtaa agaaatggat gaacgtattc gcgaatacgg ggaagaggca gcgtttgaag
    10261 tcggtgctca tacacttcat ccagatctaa tcaagatcat cggacgtctg cgattcagaa
    10321 ctagttatgg acaaaacgtc ttgaagcatt cggtcgaggt tgcaaaatta gctggtatct
    10381 tagcagcaga gcttggcgaa gatatccaat tagcaaaacg tgccgggttg ctccatgata
    10441 ttgggaaagc acttgaccat gagattgaag gatctcacgt tgagatcggt gcggaattag
    10501 ctgccaagta caaagaaaac ccagtagtaa tcaacgcgat cgcttctcat catggagatg
    10561 tggaagctac ttctgttatc tctgtccttg tagctgcagc agatgcgctc tcagcagcaa
    10621 gacctggtgc gcgaagtgaa tcattagaaa actacatccg tcgtttaaga agtctggaaa
    10681 atatttcgaa cagcttcgcc ggtgtagaat caagttacgc agttcaagct ggacgcgaag
    10741 ttcgggtaat ggtcaagcca gaagaaatct cggatttaga cgctgttcgt ttagtacgtg
    10801 atattcgtaa acgaatcgaa gaagaactgg attatcctgg acacatcaaa gtaatcgtta
    10861 ttcgggaaac tcgtgcaact gattatgcta aataataaaa aatacaaaag aagagccgcg
    10921 gcaaaagcca cagctcttct ttttttgtaa ggcacgaaaa aacattgcta tcattatttt
    10981 ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat gattgctggg
    11041 atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc aggagaagaa
    11101 acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg acaagaaccc
    11161 ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt cagtacacca
    11221 gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc agcaaggcca
    11281 atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc ttcaaaaatc
    11341 atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat cgcagaaacg
    11401 cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc ctgaggatct
    11461 tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat gaataagact
    11521 ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg tttcacggct
    11581 aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc atggacgcca
    11641 gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa cacctcttct
    11701 ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat attccctgtg
    11761 ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct ttctttttct
    11821 atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa gataacattg
    11881 gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata aacgatactt
    11941 gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt tcggctccgt
    12001 gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt ggcaagatat
    12061 ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc tggctggact
    12121 gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct tagaaaaaca
    12181 aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa caaacacgat
    12241 caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta gtttttatgg
    12301 aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac tatttatgct
    12361 gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta ttcagtcaga
    12421 aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag caccagtcat
    12481 tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc caaaaggctt
    12541 gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg tatttttgat
    12601 catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta tgttcatgat
    12661 tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt ttgacgtttt
    12721 gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag tttggctatt
    12781 atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat tgttcatcac
    12841 acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt ttttccaaaa
    12901 ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga tggtacttct
    12961 atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat tgccgttctc
    13021 attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac tcggccttgc
    13081 tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt tactatcttt
    13141 tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg tatatgtctt
    13201 gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat tcgtttattc
    13261 ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg tttacgcaat
    13321 tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc attttatttt
    13381 gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct cattgctagc
    13441 tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca gtgagtacga
    13501 aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa gccatcagct
    13561 tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc aagtcgtttt
    13621 tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg gcaatcctaa
    13681 aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag gctatcagct
    13741 tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg gttttcactt
    13801 cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt ctcggctgag
    13861 tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt ggtacgatca
    13921 actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc ttcaaggtgc
    13981 acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat ctagcggtgt
    14041 cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc cgatgacgaa
    14101 gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg aattagctga
    14161 ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg aagccaatct
    14221 aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc aagaaaaagt
    14281 catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg cttatgaaaa
    14341 gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga aaggaagtaa
    14401 tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag gaaatgttcc
    14461 gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg tttggattat
    14521 gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact aggcagacat
    14581 aagctatact tttatttttc cctatgatag aatacttgcg acttagactt ttcgaatggg
    14641 tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac tggatgtatt
    14701 atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg tagacttggc
    14761 agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa caaataaagc
    14821 atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc taaaaaataa
    14881 atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag tagcagctgg
    14941 attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc aattatggaa
    15001 tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta cagatgttcc
    15061 ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa tccaagtatt
    15121 accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg tctctacacg
    15181 tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc acggacttgc
    15241 tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa acagtttaga
    15301 agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta tgatcaagta
    15361 cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct tgtgtcaaca
    15421 ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg aagtttattt
    15481 agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac tgaggaatcc
    15541 taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag cagcaactca
    15601 tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat ggatttctat
    15661 tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa aattccgatt
    15721 tgttcattga aaggagcata taaatgaaaa aatatctttt gacagctacg gtaatgatag
    15781 gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa aaagaggatt
    15841 tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc gtaggggatg
    15901 aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc gaaccaagtg
    15961 caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt tcagacatgg
    16021 aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg atgatcgaag
    16081 cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa gaatcagaag
    16141 aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg gtattagcta
    16201 taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat aaaaaggaaa
    16261 cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat caaaaatact
    16321 ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct gcttttgctt
    16381 atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca ccagatcagg
    16441 aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac aacatcaaag
    16501 tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg aatgaaacag
    16561 gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa atcaaagcag
    16621 gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa agtatcaatt
    16681 aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt gaccttttac
    16741 tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg tgaatttgtc
    16801 attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc gttaggatta
    16861 ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg aaaaaaatta
    16921 agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc tagtacggtg
    16981 atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg tctctctgaa
    17041 aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga tatgcgaaat
    17101 cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc gcgtattttt
    17161 gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga tgaagaatcc
    17221 agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa atcagttcta
    17281 atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat ccgactggtg
    17341 agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga gcgtttcttg
    17401 ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg atcagaagac
    17461 aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg ttaggattct
    17521 ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca gctgtgatat
    17581 tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc attttaatgg
    17641 ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat tcagcaacaa
    17701 gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa gtcatctttt
    17761 taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg ccgatgtatg
    17821 ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat ttgatgtcga
    17881 tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt gccggtgcac
    17941 ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta ggcaaaagct
    18001 tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg tcaggactaa
    18061 ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc tttattgctt
    18121 tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga aaaaataact
    18181 aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct tctttttatt
    18241 ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt ttgaagaaaa
    18301 acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt gaaagtacgt
    18361 cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca tgaattgatt
    18421 tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag tgaagatttg
    18481 ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac aatcccgggt
    18541 gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa aaaatatatt
    18601 gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg atatgtttat
    18661 ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat cattgcttct
    18721 aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg cgtacctatt
    18781 gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa
    18841 ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga aagaattgaa
    18901 aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt agtggatgat
    18961 ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga attcgaatca
    19021 gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg cgccatcgat
    19081 gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa tattacggtt
    19141 gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag tttcgagtag
    19201 taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc ttaataccga
    19261 gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt
    19321 attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga gctaataccg
    19381 aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt ttaactctaa
    19441 gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg tgacttgtga
    19501 ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat ggtctatcta
    19561 tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg aaaaaatggg
    19621 tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt tggacgcacg
    19681 ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat tatataaagt
    19741 tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg tcagtgaaac
    19801 gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta aagcacaact
    19861 aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac atgctgtttt
    19921 gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca gtggcgatac
    19981 accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc
    20041 aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac gaatcatccg
    20101 tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc
    20161 tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt tgttcgatgc
    20221 gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag atatcatcga
    20281 aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt ttgaagaatc
    20341 aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga gaaagcgaat
    20401 taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga catacatcga
    20461 tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa
    20521 tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag acagtaggat
    20581 tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca agaaacatgc
    20641 agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa acgtccatat
    20701 cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag tcggtcactt
    20761 gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt
    20821 cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca gctttattgg
    20881 ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga ttgctgccgg
    20941 gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag caagacaagt
    21001 caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt gcaaaatcac
    21061 ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag ttgtgaaaaa
    21121 gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa tttttgcgtt
    21181 aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa
    21241 atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag aaagtattcg
    21301 tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata acttgatgga
    21361 gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca acgtagtcat
    21421 gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac caatcacagc
    21481 gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga ctttggacct
    21541 tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga tgggtgcgcc
    21601 acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc
    21661 accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga aatctccgat
    21721 tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga atatcattgg
    21781 acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag caggtacgat
    21841 cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac
    21901 acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta tcgagcgtct
    21961 agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt
    22021 aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca aaccagttag
    22081 tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag tccaattggg
    22141 ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga gcgtaggaat
    22201 tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt gactttttta
    22261 ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg aacgattgtt
    22321 gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag tttttctatt
    22381 tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa ggcagggatg
    22441 ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta ttatttaagt
    22501 gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc agcagggctc
    22561 atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat tagaaaaagc
    22621 atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt tattgcagga
    22681 gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt ttctttcgta
    22741 atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt attagggatc
    22801 caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta aataaaaaag
    22861 aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt attttttgat
    22921 aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac caattgccag
    22981 tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg ataccgggtg
    23041 ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata tttaccatcg
    23101 atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt acgcatttct
    23161 ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa agattttttt
    23221 acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct ataaaattca
    23281 tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta tacaaaatta
    23341 ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc aaaatataat
    23401 aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc
    23461 cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg ctgaatattt
    23521 tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc agaagcagat
    23581 ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca
    23641 gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata aactgactca
    23701 gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac atttgcatta
    23761 aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc attgaaaaca
    23821 ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga tgttttatga
    23881 attgttttct tatggaatct acaaagtcga atacgagact catgaccttt ttatgacgta
    23941 ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta cttttcacag
    24001 aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt ttttcaaagt
    24061 catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca
    24121 gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga tgttattttt
    24181 aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg gtatatggaa
    24241 gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc tcctctatca
    24301 gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca attaaataaa
    24361 aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg tgcttatcag
    24421 attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat cacaggtacc
    24481 tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa agctgtgaat
    24541 ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa
    24601 gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa
    24661 ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta
    24721 ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg
    24781 gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc
    24841 tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac
    24901 ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca
    24961 tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt
    25021 atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa
    25081 aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac
    25141 tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt
    25201 ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt
    25261 tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg
    25321 atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac
    25381 atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca
    25441 caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata
    25501 caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat
    25561 cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct atatttatat
    25621 tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg
    25681 ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg
    25741 gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag
    25801 gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat
    25861 gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca
    25921 aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa
    25981 atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag
    26041 cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa
    26101 aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga
    26161 attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc
    26221 gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg
    26281 gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa
    26341 aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta
    26401 ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa
    26461 aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta
    26521 gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta
    26581 caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg
    26641 atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt
    26701 aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat
    26761 attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa
    26821 gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga
    26881 aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt
    26941 acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga
    27001 cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata
    27061 tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa ttggatttct
    27121 tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa
    27181 tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt aagccaaatt
    27241 tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga aactaatcgg
    27301 cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata ataaccccaa
    27361 ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt gatgcaggac
    27421 agctttttta cgacctcttg gtacagggta gcttttttac caatttctag ctataattaa
    27481 agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac aaaaagacta
    27541 gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct ggtccatccg
    27601 gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat gattttcagt
    27661 attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc gttgattatt
    27721 tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg ctggaatacg
    27781 cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag acattggatg
    27841 aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc aaagaaaaag
    27901 tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta aaatcacgga
    27961 tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa gttgcaaaag
    28021 aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa gtgccgctag
    28081 cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat cgcgtaatcg
    28141 gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc ttcaattgac
    28201 tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag taaacgcgcg
    28261 catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt aaaaagcgtc
    28321 ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca tcctgaatta
    28381 aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga gcgtgaacaa
    28441 cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa aatagagcga
    28501 acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc acttattgtc
    28561 tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt atctgctgtt
    28621 aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta gtaaaatagg
    28681 caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg aaccatgaca
    28741 ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc attcacttat
    28801 ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga agtccctttt
    28861 ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc agaagaatca
    28921 gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt ggtcaatgaa
    28981 gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa aatcacttgt
    29041 ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat ctatcctttg
    29101 gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc ctggaaggaa
    29161 gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca attagtagat
    29221 atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt ccgttctttg
    29281 ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc caagaaacaa
    29341 aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc ttatttcaaa
    29401 aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg gctggagttt
    29461 atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca aacgacggca
    29521 ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc aggtcagcag
    29581 caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac agaagtatat
    29641 cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt agtgcctgaa
    29701 atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga atcagttgcg
    29761 gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa gattgagcgg
    29821 gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct gaaaaatatc
    29881 gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga gacaccgcgt
    29941 tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc aatagttcta
    30001 ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt ttatcagtta
    30061 ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga gatcgtagat
    30121 atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt gcaggaaaaa
    30181 atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg tcgaggatat
    30241 tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa ttgcgatatc
    30301 tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg acatgaagaa
    30361 cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta tggaacaggt
    30421 actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat tttgcgtatg
    30481 gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa attcggtgca
    30541 aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga ctttccagaa
    30601 gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga ttttcgttct
    30661 agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg agcggaaaaa
    30721 gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga attggctaaa
    30781 aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg tggaggctat
    30841 cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca agtagcggca
    30901 aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca aagcttgcta
    30961 ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta tcaactaatc
    31021 atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt gaatggaagc
    31081 cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt aaactttatt
    31141 taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat aaattaaaaa
    31201 gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta gacaatttat
    31261 atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta ggacaaaaca
    31321 agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt aacccagaaa
    31381 tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt ccgcatgtct
    31441 atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga gacggagaag
    31501 agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa atcgatcact
    31561 tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa gaacttgaag
    31621 aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc tgctttttct
    31681 gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt ggtaacacag
    31741 cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa agaagcggct
    31801 ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc agaaatggaa
    31861 gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca attcttgcct
    31921 gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc tttacttcct
    31981 aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa agaaactggt
    32041 gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc gcaagaaagc
    32101 ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag ccttttagga
    32161 agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc agtacctcaa
    32221 gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc aatcgattgg
    32281 cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg gccaattgca
    32341 ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt ggcagaaact
    32401 acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg gattgcttgc
    32461 ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg aaaacaggcc
    32521 gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca ggtgaaataa
    32581 tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta ctgacattag
    32641 agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg atcaaacaaa
    32701 gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt acgattagcc
    32761 atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa aaagtggatg
    32821 actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta gataaagtcc
    32881 ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga aatcctggga
    32941 cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca ccagatctgc
    33001 aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct ctatggatga
    33061 ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt ttatcgctat
    33121 atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga gatcaggcca
    33181 tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt ccatacggta
    33241 tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc gtcatgacga
    33301 tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct gaacatcatg
    33361 tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct tttttagatc
    33421 cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc aaactcattg
    33481 aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag atggatgcta
    33541 gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta gacgcgcctt
    33601 gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa cagccggatg
    33661 aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca ccaactttaa
    33721 agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa aaccaagacg
    33781 ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt ttgaccaatc
    33841 ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat cagttttaca
    33901 cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa atgcagattg
    33961 aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc agtgtattta
    34021 ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt catcgtgccg
    34081 gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa aaacaagaag
    34141 ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa gaaaatatgc
    34201 tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca acaatcgtag
    34261 cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc agagcttatt
    34321 tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc aatgaactag
    34381 tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa aatattttaa
    34441 cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat ctttggcaac
    34501 taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct gaagaagaaa
    34561 taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag ctgattgatc
    34621 gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa tataaggagg
    34681 acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca ctggaaatat
    34741 cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg atcgtgatgt
    34801 agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc gacgatttca
    34861 gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg tttacgatgt
    34921 tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa tggacttgaa
    34981 acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata ttatgcagca
    35041 gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag atttgaaacc
    35101 ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg ggattgctat
    35161 cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg ttcattatct
    35221 gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt atgctttagg
    35281 gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat ctgctgtaac
    35341 aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg atccaaatgt
    35401 gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg cagaccgtta
    35461 taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag ctcgtgcaaa
    35521 cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca caccccttac
    35581 ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag acaagccaga
    35641 aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga aaggacagaa
    35701 aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa cagctgtatt
    35761 ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc tttctgaagc
    35821 ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa cagaagaaat
    35881 tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag cgggaacgac
    35941 agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa aaatcaaatt
    36001 agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag aattagggtt
    36061 ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa gcggaatgat
    36121 catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca cgattacttt
    36181 cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag aaaactacga
    36241 aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta gtgctactta
    36301 tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag cctcgggtga
    36361 agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag agcctaaatt
    36421 gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta gtgtcggtgc
    36481 agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag ataaagtgat
    36541 tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga acgtaattta
    36601 ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag acagttctag
    36661 cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt ccagctccac
    36721 aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa tatatcagaa
    36781 gggaatgcga cattgatttg ttgcattct
//
LOCUS       DADXTH010000022        36563 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.022, whole genome shotgun sequence.
ACCESSION   DADXTH010000022 DADXTH010000000
VERSION     DADXTH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.022"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            359..1375
                     /locus_tag="IWI97_001046"
     CDS             359..1375
                     /locus_tag="IWI97_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBC2462566.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1516..2817
                     /locus_tag="IWI97_001047"
     CDS             1516..2817
                     /locus_tag="IWI97_001047"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBC2462567.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2863..3525
                     /gene="deoC"
                     /locus_tag="IWI97_001048"
     CDS             2863..3525
                     /gene="deoC"
                     /locus_tag="IWI97_001048"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBC2462568.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3546..3944
                     /locus_tag="IWI97_001049"
     CDS             3546..3944
                     /locus_tag="IWI97_001049"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBC2462569.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            4010..5092
                     /locus_tag="IWI97_001050"
     CDS             4010..5092
                     /locus_tag="IWI97_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2462570.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5304..6866
                     /locus_tag="IWI97_001051"
     CDS             5304..6866
                     /locus_tag="IWI97_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462571.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6859..7992
                     /locus_tag="IWI97_001052"
     CDS             6859..7992
                     /locus_tag="IWI97_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462572.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7992..8948
                     /locus_tag="IWI97_001053"
     CDS             7992..8948
                     /locus_tag="IWI97_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462573.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            9047..10213
                     /gene="deoB"
                     /locus_tag="IWI97_001054"
     CDS             9047..10213
                     /gene="deoB"
                     /locus_tag="IWI97_001054"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBC2462574.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10237..11055
                     /locus_tag="IWI97_001055"
     CDS             10237..11055
                     /locus_tag="IWI97_001055"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBC2462575.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            11072..11779
                     /gene="deoD"
                     /locus_tag="IWI97_001056"
     CDS             11072..11779
                     /gene="deoD"
                     /locus_tag="IWI97_001056"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBC2462576.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(12077..12763)
                     /gene="gpmA"
                     /locus_tag="IWI97_001057"
     CDS             complement(12077..12763)
                     /gene="gpmA"
                     /locus_tag="IWI97_001057"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBC2462577.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(12935..13516)
                     /locus_tag="IWI97_001058"
     CDS             complement(12935..13516)
                     /locus_tag="IWI97_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2462578.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(13649..14332)
                     /gene="rpiA"
                     /locus_tag="IWI97_001059"
     CDS             complement(13649..14332)
                     /gene="rpiA"
                     /locus_tag="IWI97_001059"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBC2462579.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            14651..15064
                     /gene="rpsL"
                     /locus_tag="IWI97_001060"
     CDS             14651..15064
                     /gene="rpsL"
                     /locus_tag="IWI97_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBC2462580.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            15148..15618
                     /gene="rpsG"
                     /locus_tag="IWI97_001061"
     CDS             15148..15618
                     /gene="rpsG"
                     /locus_tag="IWI97_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBC2462581.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            15712..17799
                     /gene="fusA"
                     /locus_tag="IWI97_001062"
     CDS             15712..17799
                     /gene="fusA"
                     /locus_tag="IWI97_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBC2462582.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            17971..19158
                     /gene="tuf"
                     /locus_tag="IWI97_001063"
     CDS             17971..19158
                     /gene="tuf"
                     /locus_tag="IWI97_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBC2462583.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            19564..19872
                     /gene="rpsJ"
                     /locus_tag="IWI97_001064"
     CDS             19564..19872
                     /gene="rpsJ"
                     /locus_tag="IWI97_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBC2462584.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            19903..20532
                     /gene="rplC"
                     /locus_tag="IWI97_001065"
     CDS             19903..20532
                     /gene="rplC"
                     /locus_tag="IWI97_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBC2462585.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            20560..21183
                     /gene="rplD"
                     /locus_tag="IWI97_001066"
     CDS             20560..21183
                     /gene="rplD"
                     /locus_tag="IWI97_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBC2462586.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            21183..21473
                     /gene="rplW"
                     /locus_tag="IWI97_001067"
     CDS             21183..21473
                     /gene="rplW"
                     /locus_tag="IWI97_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBC2462587.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            21512..22345
                     /gene="rplB"
                     /locus_tag="IWI97_001068"
     CDS             21512..22345
                     /gene="rplB"
                     /locus_tag="IWI97_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBC2462588.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            22388..22666
                     /gene="rpsS"
                     /locus_tag="IWI97_001069"
     CDS             22388..22666
                     /gene="rpsS"
                     /locus_tag="IWI97_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBC2462589.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            22689..23036
                     /gene="rplV"
                     /locus_tag="IWI97_001070"
     CDS             22689..23036
                     /gene="rplV"
                     /locus_tag="IWI97_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBC2462590.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            23050..23706
                     /gene="rpsC"
                     /locus_tag="IWI97_001071"
     CDS             23050..23706
                     /gene="rpsC"
                     /locus_tag="IWI97_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBC2462591.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            23709..24143
                     /gene="rplP"
                     /locus_tag="IWI97_001072"
     CDS             23709..24143
                     /gene="rplP"
                     /locus_tag="IWI97_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBC2462592.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            24133..24321
                     /gene="rpmC"
                     /locus_tag="IWI97_001073"
     CDS             24133..24321
                     /gene="rpmC"
                     /locus_tag="IWI97_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBC2462593.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            24346..24612
                     /gene="rpsQ"
                     /locus_tag="IWI97_001074"
     CDS             24346..24612
                     /gene="rpsQ"
                     /locus_tag="IWI97_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBC2462594.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            24670..25038
                     /gene="rplN"
                     /locus_tag="IWI97_001075"
     CDS             24670..25038
                     /gene="rplN"
                     /locus_tag="IWI97_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBC2462595.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            25075..25383
                     /gene="rplX"
                     /locus_tag="IWI97_001076"
     CDS             25075..25383
                     /gene="rplX"
                     /locus_tag="IWI97_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBC2462596.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            25411..25950
                     /gene="rplE"
                     /locus_tag="IWI97_001077"
     CDS             25411..25950
                     /gene="rplE"
                     /locus_tag="IWI97_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBC2462597.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            25969..26154
                     /locus_tag="IWI97_001078"
     CDS             25969..26154
                     /locus_tag="IWI97_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBC2462598.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            26190..26588
                     /gene="rpsH"
                     /locus_tag="IWI97_001079"
     CDS             26190..26588
                     /gene="rpsH"
                     /locus_tag="IWI97_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBC2462599.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            26620..27156
                     /gene="rplF"
                     /locus_tag="IWI97_001080"
     CDS             26620..27156
                     /gene="rplF"
                     /locus_tag="IWI97_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBC2462600.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            27317..27673
                     /gene="rplR"
                     /locus_tag="IWI97_001081"
     CDS             27317..27673
                     /gene="rplR"
                     /locus_tag="IWI97_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBC2462601.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            27694..28194
                     /gene="rpsE"
                     /locus_tag="IWI97_001082"
     CDS             27694..28194
                     /gene="rpsE"
                     /locus_tag="IWI97_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBC2462602.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            28209..28388
                     /gene="rpmD"
                     /locus_tag="IWI97_001083"
     CDS             28209..28388
                     /gene="rpmD"
                     /locus_tag="IWI97_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBC2462603.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            28432..28872
                     /gene="rplO"
                     /locus_tag="IWI97_001084"
     CDS             28432..28872
                     /gene="rplO"
                     /locus_tag="IWI97_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBC2462604.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            28872..30167
                     /gene="secY"
                     /locus_tag="IWI97_001085"
     CDS             28872..30167
                     /gene="secY"
                     /locus_tag="IWI97_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBC2462605.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            30229..30876
                     /locus_tag="IWI97_001086"
     CDS             30229..30876
                     /locus_tag="IWI97_001086"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBC2462606.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            31067..31285
                     /gene="infA"
                     /locus_tag="IWI97_001087"
     CDS             31067..31285
                     /gene="infA"
                     /locus_tag="IWI97_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBC2462607.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            31318..31434
                     /gene="rpmJ"
                     /locus_tag="IWI97_001088"
     CDS             31318..31434
                     /gene="rpmJ"
                     /locus_tag="IWI97_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBC2462608.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            31452..31817
                     /gene="rpsM"
                     /locus_tag="IWI97_001089"
     CDS             31452..31817
                     /gene="rpsM"
                     /locus_tag="IWI97_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBC2462609.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            31845..32234
                     /gene="rpsK"
                     /locus_tag="IWI97_001090"
     CDS             31845..32234
                     /gene="rpsK"
                     /locus_tag="IWI97_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBC2462610.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            32314..33252
                     /locus_tag="IWI97_001091"
     CDS             32314..33252
                     /locus_tag="IWI97_001091"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBC2462611.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            33280..33660
                     /gene="rplQ"
                     /locus_tag="IWI97_001092"
     CDS             33280..33660
                     /gene="rplQ"
                     /locus_tag="IWI97_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBC2462612.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            33932..34771
                     /locus_tag="IWI97_001093"
     CDS             33932..34771
                     /locus_tag="IWI97_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBC2462613.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            34747..35616
                     /locus_tag="IWI97_001094"
     CDS             34747..35616
                     /locus_tag="IWI97_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBC2462614.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            35606..36403
                     /locus_tag="IWI97_001095"
     CDS             35606..36403
                     /locus_tag="IWI97_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBC2462615.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        11761 a         6679 c         8070 g        10053 t
ORIGIN      
        1 atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata actagctgaa
       61 aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg tatcagttct
      121 ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa gaaatagtag
      181 gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt atagaaggct
      241 gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt tttctctatt
      301 cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag ccaataggat
      361 gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca
      421 gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag tgttgtcgat
      481 cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca atcgacattc
      541 aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca aactgatcga
      601 aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca attctcgtca
      661 tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag tggacggaat
      721 cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg gtttttatca
      781 aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg gacggcttat
      841 cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa atggccatac
      901 gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac gtttcaacgg
      961 atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg ttgaacatgg
     1021 agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac ataaagatat
     1081 cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg cgatcgatga
     1141 attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat taaagctgtc
     1201 agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata ttggatttta
     1261 tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc cgaacaaagt
     1321 gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg
     1381 cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt atttttagta
     1441 aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc gaatgaagac
     1501 aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga tggaaacgag
     1561 ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa gattccagat
     1621 tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa tgaagaaatc
     1681 acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag ttctcttgaa
     1741 ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac attgatttta
     1801 gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg cggtctaggt
     1861 tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat cgagctttcg
     1921 gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg tcaatcagga
     1981 aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc gaccgttgat
     2041 tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca
     2101 atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga agatgcaaga
     2161 cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac agtggcggtt
     2221 atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga agttgtcgag
     2281 gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg ctacgcctta
     2341 ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg
     2401 cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat
     2461 caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc acgctacaca
     2521 atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga
     2581 attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat tgatcacgct
     2641 gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga gtcactattg
     2701 acgatctata gtaacgacga agaaatcact tctgtcattg aattattata taaaaatatt
     2761 gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac agaataaagg
     2821 aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact aaaccgaatg
     2881 atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca aattatcgaa
     2941 gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg
     3001 aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt tcctctagga
     3061 gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa cggtgcagat
     3121 gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca acaagtccaa
     3181 aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa agttattatt
     3241 gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc aaaagctgca
     3301 ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa ggtagaggat
     3361 gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc
     3421 cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat cggaacaagc
     3481 gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt tcaaaggaga
     3541 agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg ttagataaag
     3601 cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga
     3661 tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt gccgagcgca
     3721 cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg
     3781 ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg gcagaattct
     3841 gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag gcaacgaccg
     3901 ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg ttcattcagt
     3961 cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa tgaaaaaagc
     4021 aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg
     4081 caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa caactgcagc
     4141 attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag cttgggaagg
     4201 actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct tccaatattt
     4261 ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga atgctggatt
     4321 caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac aagcaacaaa
     4381 caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag ataacgttgt
     4441 atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag cagcttatac
     4501 gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt
     4561 tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa
     4621 agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt caattgctca
     4681 aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta caggtaacgg
     4741 tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt gggttatcgg
     4801 tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa agaaaaactt
     4861 cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg cacaaaaatc
     4921 agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag aagacggtgt
     4981 tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga
     5041 aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact aaaaccattt
     5101 ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac aagaattgtt
     5161 catttatcca agctgccttg tgacagtttg gataagggaa caactccctg aagtaatgtt
     5221 tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc cctagataga
     5281 aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa atgcagaata
     5341 ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa gtaaaggcag
     5401 gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg atgaacgtgc
     5461 tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa gaagtaaaga
     5521 tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag cactttatgc
     5581 ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag
     5641 ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa cagtatggat
     5701 tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa caacgggtag
     5761 aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag ccgacagctg
     5821 tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag
     5881 gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta gctgaccgct
     5941 gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac
     6001 aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg aaaaaagaag
     6061 cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat
     6121 tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg
     6181 gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg cgaaaggcag
     6241 aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc cgaaaaatca
     6301 ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta gtcctagata
     6361 tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac agtaaaaacg
     6421 gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa gaatatgatg
     6481 ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat cagcaaaaag
     6541 caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca aatccaactc
     6601 gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa caaagggaca
     6661 aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat gtttcggatc
     6721 gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg
     6781 aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga aaagagttag
     6841 aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt attagtgcct
     6901 gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc ttttggtttt
     6961 cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca aagagggata
     7021 ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt ttctgtagct
     7081 aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg gtggatcgtc
     7141 agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct tccgttatgc
     7201 gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt acttcgagca
     7261 ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt attatacgta
     7321 agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt ggactcttcc
     7381 aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc ttttggcggc
     7441 tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt ttggctgctg
     7501 atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc ttttgcttct
     7561 gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat ttctggcgct
     7621 ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa
     7681 acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg ttctggatca
     7741 gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg tggacttggc
     7801 atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc tttgattatc
     7861 ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa
     7921 aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg
     7981 ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct acactggttt
     8041 attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga agcgggatcg
     8101 taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc gttttcaact
     8161 tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta gtgggtggcg
     8221 tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg cgtgctgacc
     8281 atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt ttcttgatca
     8341 aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg tatttttctt
     8401 tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat acattcttac
     8461 ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc aagacgaaat
     8521 tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca ttaggtatca
     8581 atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg
     8641 gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc gttggacaag
     8701 ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg gcaatgggtg
     8761 cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa ttaccagtta
     8821 tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca atcattgtgc
     8881 tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac tatatcaaat
     8941 caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt cgtctcgccc
     9001 tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt
     9061 acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg ataagttcgg
     9121 tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga acgttccaca
     9181 tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga
     9241 tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata cgatgacagg
     9301 ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc ctaatggatt
     9361 ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa
     9421 accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga aaacaggcga
     9481 tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg aagaaatcat
     9541 tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa aagatgatcc
     9601 gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg
     9661 aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc ttgattcttt
     9721 gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt tcaacggaca
     9781 aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc agctgctgaa
     9841 agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt ttgatgcatt
     9901 atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat ttgacttacg
     9961 aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta cagccgatca
    10021 cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac cgttattggt
    10081 ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt cagatatttc
    10141 agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa
    10201 attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag cgagatgtta
    10261 caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt tggtttgatt
    10321 ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat gatttcttat
    10381 gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa gctagtttat
    10441 gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta ctatgaaggt
    10501 cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg ggcccattca
    10561 gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg agatttgatg
    10621 ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc gaatgaagat
    10681 gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta tcaagcaaca
    10741 gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata tatgggctat
    10801 tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat aggagccgat
    10861 gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg attaaaagtg
    10921 ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct gagccatgaa
    10981 gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt taaagaaaca
    11041 ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag cgaaagaagg
    11101 cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat atattgcgga
    11161 gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag gctttaccgg
    11221 caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc catcagccac
    11281 catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc gagtaggtac
    11341 atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac aaggtgcagc
    11401 gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc caatcagtga
    11461 tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata cggttcatgt
    11521 aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga cgttccaatt
    11581 agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt acttaggcgc
    11641 gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga tcaccggtga
    11701 agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag taggattgga
    11761 aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag taataagaac
    11821 atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat tgcaaatgta
    11881 agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct gagtcacaag
    11941 gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgtcttgt gaccgagaag
    12001 ttggcaggag aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg tcacagtccc
    12061 ttttttatag tgttttttat aggtagtatt ttttagcaac tgttaagtca tcatttaatt
    12121 cataaacaag tggttgacct gttggaattt caagatccat gatgtcttcg tcagagatac
    12181 cttcgatatg tttagctagg gcgcgtaatg agttaccatg tgcagctact aaaacagttt
    12241 tgttgtctag taatgctgga gcaatttcat cttgccagaa tggcaaagcg cgttctaatg
    12301 tcactttcaa gttttctcca ccaggaatat cgcgtttgtc taacattgcg taacgacggt
    12361 catttgctgc agaaccttcg tcagtttctt ccattaatgg aggaagagta tcgtaagaac
    12421 gacgccagat gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac
    12481 cttgtaattt accatagtga cgttcgttta agcgccatga tttgatttgt ggtacccaaa
    12541 gttgatctga atattctaag atcaagttac atgttttgat tgcacgagtt aaaacagaag
    12601 tgtaagcaac atcaaattcg attccagctt ctttgatttt gcgtccgcct tcttttgctt
    12661 cttcgattcc ttctggtgct agatttacat cagcccaacc agtaaattgg ttcaatgcat
    12721 tccattcact aagaccgtga cgagaaaaaa ctaattttgg catgtttagc tctcctttta
    12781 agataggttt atatttctga aattttcagt tctgttttat tgtacaattt tttggaagag
    12841 atttccatcg aaaaattaga taagattcta tgagaaaaca agtagagaat ataaaaaaac
    12901 gaacagtgcc cacgcaactg ttcgttttta ggtattagat gcataattgc tcgatttctt
    12961 tttcaggtaa attgatatat ttataacatg ttccaactga aacaccgcat tttgaagaaa
    13021 taaattggat cgtttctttt ttttcataat agagacgtcg gatttttttg atcgtttttg
    13081 catcgatttt tggtcttccg ccgatttttc cttttttacg agcttcatct aatccaacta
    13141 atgtacgttc tttgatcaat gcgcactcca tgtttgctag gttttccatt aattggaaat
    13201 agatttttcc catttcgcgc gtatcgattc cttcatcaag actaatcaag tgcagcccgc
    13261 gatctttgaa taattgcgtg agttctgtta attgccgcgt agatttgcct aatcgatgaa
    13321 gttggcaaat gactagtgca tcgccttgtt gcatgtcagc gatcactgac tccaaatgaa
    13381 cagcagtaga atcattttgt tccacttcat atgtctcatg gaaaatttct tgacaaccaa
    13441 agtcagataa ttgatcaacc tgagttgtta aatcaacgtc atttatggtt gtgcgtgcat
    13501 aaccaataat tttcatggtg attcctttct agcagtgatt ttctaactgg atataacagt
    13561 aaaaatgtag cataaagtac gtcaaaaaga tagttttttc gcaaattttc cgctgtcttt
    13621 ttttattgga tgatcaagca gtttttcttt atttacgacg gattgcgtca agaatctcaa
    13681 cgcctttttc tgagccgaca actactttag aaaccatgtt taggaaaagc ccatgttcta
    13741 ctaccccaac aagttcatct aaataggtag ctaagctttc tggctgttcg atgacttcta
    13801 gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa gacttctccg ttttcgtctg
    13861 tacgcaagac gggttggaat cttttttctt caaaaatatg aagtagctgt tgggaaccat
    13921 aagggatcac ttcaactggg agtgggaact tcccaagttt atcgactaat ttagaagaat
    13981 caacgatcca aatcgtttct tttgagtacg tagcgactat tttctcgaaa agcaaagcag
    14041 cgccgccgcc tttgatccct tgaaaatcag cactgatttc atcagcaccg tctattgtca
    14101 aatcgactac ggggacttca tcaatactct ttaacggtat tcctaatttt tccgcttgtt
    14161 cttttgtcgc atttgaagta gtaactcctg tgatacgtaa accttcttca cgcatacggc
    14221 gaccaatctc ttctaccata taataggcgg ttgaaccggt cccgagacct acgatcatcc
    14281 cgtcctttac gaatttcgcg gcttcaatgc cagctaattg ttttaaattc atctttgcgt
    14341 ccaccttttt ttagtttgag catcgtgctt tcctcactat tttagcgtga tttacggtaa
    14401 aaagatacca ttctttttta ttccaataaa gctttttcaa aaaaaattgc atctcttgtt
    14461 gacatgtttt ttcattcgtg atattctaac aaaggtgctt tatctacagg tttctgtgaa
    14521 gaaacgtgct gactgtttat caaagcatat ttgataagga tgcagacatt gcatttattt
    14581 tttatcacaa aaaaagaacc acctggatgt gtggaactag acgcaaatcg tgggaaggag
    14641 gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat caaaggtgga
    14701 aaaatctaat tcacctgctt tgaacaaagg atataacagc tttaagaaaa ctcaaactaa
    14761 cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga cacctaaaaa
    14821 accgaactca gctttacgta aatatgcacg tgttcgtttg tcaaacttaa ttgaagttac
    14881 tgcttatatc ccagggattg gtcataactt acaagaacac agtgttgtat tattacgcgg
    14941 tggtcgtgta aaagacttac caggggtacg ttaccatatc gttcgcggtg ctttagatac
    15001 tgctggtgtt aacgaccgta aacaaagccg ttctaaatac ggaactaaaa aacctaaagc
    15061 ttaattttaa ttattaaaca aatgaaactt gcttctacta tatagaagta attactatga
    15121 acaatatttc ggaaggagga gttacggatg ccacgtaaag gtcctgttgc aaaacgtgat
    15181 gttttaccag atcctattta taactcaaaa ttagtaactc gcttgatcaa ccgtgtaatg
    15241 gttgacggaa aacgcgggat tgcagctaac attatctata atgcctttga cttgatcaaa
    15301 gaatctacag gtaacgatcc attagaagtg tttgaacaag caatgaaaaa cgttcaacct
    15361 gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact accaagtacc agttgaagtt
    15421 cgccctgaac gccgcgttac tctaggtctt cgttgggtag taaactacgc tcgcttgcgc
    15481 ggtgaacaca caatggaaga acgcttagca aaagaaatca tggatgctgc aaacaacaca
    15541 ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa ccgtgcgttt
    15601 gctcactatc gctggtaaga ttctctctgt ttggtcggct tttataagtc gacctcagat
    15661 agtgtcctta tttttagcaa ctagatagta aagagaggag aaacaaggag aatgaccaga
    15721 gaattttctt tacaaaacac tcgtaatatc ggtatcatgg cccacatcga tgctggtaaa
    15781 acgactgcta ctgaacgtat cttgtactat actggtaaaa tccataaaat tggtgaaact
    15841 catgaaggag cttcacaaat ggactggatg gaacaagaac aagaacgtgg tattacgatc
    15901 acttctgctg ctactacagc acaatggaag ggtcaccgaa tcaacatcat cgacacacca
    15961 ggacacgtag acttcacagt tgaagttgaa cgttcattgc gtgtacttga tggtgctgta
    16021 actgttcttg atgcacaatc aggtgttgaa cctcaaactg aaacagtttg gcgtcaagca
    16081 acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat cggtgctgac
    16141 ttcttatatt cagtatcaac tttacatgac cgtttgcaag caaacgctca cccaatccaa
    16201 ttaccaattg gtgcggaaga cgacttcaca ggaattatcg accttgtaac aatgaaggct
    16261 gaaatgtata caaacgatct tggaacagag atcgaagaaa cagaaattcc tgaagaatac
    16321 cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga aactgatgaa
    16381 gaacttactt tgaaatactt agaaggtgaa gaaatcactg aagctgagtt aaaagaaggt
    16441 atccgccgcg caacagttaa cgtagaattc tacccagtac tatgtggttc agcatttaag
    16501 aacaaagggg ttcaattact attggacgct gttcttgatt acttgccatc accgttagat
    16561 atcccagcta tcaaagggat tgaccctaaa actgatgaag aagtagaacg tcctgcagac
    16621 gacagcgctc ctttctcagc tttagcattt aaagttatga ctgacccatt cgtaggtcgt
    16681 ttaactttct tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt acaaaacgca
    16741 acaaaaggca aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa ctctcgttca
    16801 gaaatctctg aagtttacgc tggagatatc gctgctgctg ttggtttgaa agacacaact
    16861 actggggaca cactatgtga tgaaaagagt ctagttattc ttgaatccat ggaattccca
    16921 gaaccagtta tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga taaaatgggt
    16981 gtggctttac aaaaactatc tgaagaagat cctactttcc gtgctgaaac aaacgtggaa
    17041 acaggcgaaa caatcatcgc tggtatgggt gaacttcact tagatatcat cgttgaccgt
    17101 atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc ttaccgtgaa
    17161 actttccgtg ctggcactca agccgaaggt aagtttgtac gtcagtctgg tggtaaaggt
    17221 caatacggtc acgtatggat tgaatttaca ccaaatgaag aaggtgctgg tttcgaattt
    17281 gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt tgaaactggt
    17341 ttgaaagatg ctatggaaaa tggtgtctta gctggctatc cattagtaga tatcaaagct
    17401 aaactttacg atggttcata ccatgatgtc gattcaaatg aaactgcctt ccgtgttgct
    17461 gcttctatgg cattacgtgc agcagctaag aaagctaatc cagtaatcct tgaaccaatc
    17521 atggctgtag aagttgttat ccctgaagat tatttaggtg atgttatggg tcacgtaact
    17581 gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg gcggccaaca agttgttcgt
    17641 gcgatggttc cacttgctga aatgtttggt tatgctacaa ctcttcgttc tgctactcaa
    17701 ggtcgtggta catttacaat gacatttgac cactacgaag atgtaccaaa atcagtacaa
    17761 gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat ttcttaaaac cggatagtcc
    17821 tgcaaagaga ttatccaaag agactatatt tttaatatag tttcgtgtaa aataattatc
    17881 gatgatatgg gcatgtggtg taccttgtgt acatcatata cctatcagac aaataaaaaa
    17941 tatattgtat ttttaggagg acattttaaa atggcaaaag aaaaatttga ccgttctaaa
    18001 ccacacgtta acattggtac tatcggacac gttgaccatg gtaaaactac attaacagct
    18061 gcaatcacaa ctgtactatc taagaaaaac ggcggccaag ctatggctta cgatcaaatc
    18121 gatggtgctc cagaagaacg cgaacgcgga atcacaatct caacagctca cgttgagtat
    18181 gaaacagata cacgccacta cgctcacgtt gactgcccag gacacgcgga ttacgttaaa
    18241 aacatgatca ctggtgctgc tcaaatggac ggagctatct tggtagtttc tgctgctgac
    18301 ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg tgttccttac
    18361 atcgttgtat tcttgaacaa agtagacatg gttgatgacg aagaattact agaattagtt
    18421 gaaatggaag ttcgtgacct attaacagaa tacgaattcc ctggtgacga tgttcctgta
    18481 gttgctggat cagctttgaa agctctagaa ggcgacgctt catacgaaga aaaaattctt
    18541 gaattaatgg ctgcagttga cgaatacatc ccaactccag aacgtgacaa cgacaaacca
    18601 ttcatgatgc cagttgaaga cgtgttctca attactggac gtggtactgt tgctacaggt
    18661 cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg aagttgttgg tattgctgaa
    18721 gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt agactacgct
    18781 gaagctggag acaacattgg tgctttacta cgtggtgttg cacgtgaaga catccaacgt
    18841 ggacaagttt tagctaaacc aggtacaatc acacctcata caaaattctc tgcagaagta
    18901 tacgtgttga caaaagaaga aggtggacgt catactccat tcttcactaa ctaccgtcca
    18961 caattctact tccgtacaac tgacgtaaca ggtgttgttg aattaccaga aggaactgaa
    19021 atggttatgc ctggcgacaa cgtaacaatg gaagttgaat taatccaccc aatcgctatc
    19081 gaaaacggta ctaaattctc aatccgtgaa ggcggacgta cagttggtgc cggtgtcgtt
    19141 acagaaatca aagcttaatt cacttaagtt ttaaagtaaa gcaacattta ttcggacgta
    19201 atcgctcgga acgaaggtct caggatcttt gttccgagct tttttattta ttgcaggatc
    19261 aggaaaaaag acaggagtat tataatagaa ggaacgcgaa ataaaagagt cagtattttc
    19321 taaatgaaac tttatgaaaa caaaatgttt tttacaagaa aaagccttgc tttgtgagtt
    19381 tatatatatt ataatgacaa aggtgcgttt actttagtga attgcacaga cgtaatcaga
    19441 atgatttcgt cggctaagaa acgagaggtt gcgacacgcc cggacgcttt gccacgaacg
    19501 agcgtgacgg aaattttcgt ggagctatgt ctacttttca aaataggcga aggagggaac
    19561 aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt atgaacaccg tattttagat
    19621 caatcagcgg ataaaatcgt ggaaactgca aaaagaactg gagctagcgt gtctggtcca
    19681 attccattac caactgaacg cagtctttac acaatcatcc gtgcgactca taaatacaaa
    19741 gattcacgcg aacaattcga aatgcgtaca cacaaacgtc ttatcgacat tgtgaaccca
    19801 acaccaaaaa ctgttgatgc tttgatgaag ctagacttac catctggtgt aaacatcgaa
    19861 atcaaattat aaaactaaaa caaaaataat ggaggtgtac tcatgaccaa aggaatctta
    19921 gggaaaaaag tgggaatgac acaaatcttc actgaatctg gtgaattaat tccagtaact
    19981 gttgtagaag ctactccaaa cgtagtacta caagtgaaaa caatggaaac ggatggctac
    20041 gaagctattc aagttggtta ccaagatatg cgtgaagttt tatcaaacaa acctgcgaaa
    20101 ggtcatgttg caaaagcaaa cacggctcct aagcgcttca ttcgtgaatt caaaaatgtt
    20161 gagctaggag aatatgaagt aggaaaagaa attacagtag atgttttcca agcaggagac
    20221 attattgatg taacaggtac tacgaaaggt aaaggattcc aaggggttat caaacgccac
    20281 ggacaaagcc gcggaccaat ggctcacggt tctcgttacc atcgtcgtcc tgggtcaatg
    20341 ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc ttgctggccg tatgggtggc
    20401 aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg atgcagaaag aaatgttatc
    20461 ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa tcactatcaa atcagctgtg
    20521 aaagctaaat aattgtagga agaaaggagg aactaaggaa tgccgaatgt agcattattc
    20581 aaacaagatg gaagccaaaa cggtgaaatc acacttaacg aagaaatctt cggaattgaa
    20641 cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac gtgcgtcatt aagacaagga
    20701 acacacgcgg ttaaaaaccg tagcgctgtt cgtggcggtg gacgtaaacc atggcgtcaa
    20761 aaaggaacag gtcgtgcacg tcaaggatct atccgctcac cacaatggcg tggaggtggc
    20821 gtagtctttg gaccaacacc acgttcttac agctacaaac ttcctaaaaa agttcgtcgt
    20881 ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa acaacttggt agcaatcgaa
    20941 ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac aagttcttgc taacttatct
    21001 atcgactcta aagtattggt tgttctagaa aaaggtaacg actttgctgc tttatcagca
    21061 cgtaacttac caaacgtttc tgtagtaact tctgataatg tgagtgtgct agacgttgta
    21121 tcaaatacaa aagtgttagc aacacaaact gctcttactc aaattgagga ggtgcttgca
    21181 taatgaactt actagacgta atcaaacgcc cagtgatcac tgaaaaatca atgcttgcca
    21241 tggatgacaa gaaatacact tttgaagtgg acactcgcgc aaacaaaact ttagtaaaac
    21301 aagcggtaga agctgctttt gacgttaaag tcaaaaacgt gaacatcgtg aacgtacgtg
    21361 cgaaattcaa acgtatgggc aaatatgcag gatatacaaa aaaacgtcgc aaagcgatcg
    21421 ttacattgac tgaagattca aaagaaatcc aattattcga agctgctgaa taagcactaa
    21481 tagataactc attaaattag gagggaaaaa cgtggcgatt aaaaagtaca aacctacctc
    21541 aaatggtcgt cgtaacatga caggttctga ttttgctgaa atcacaacat caacacctga
    21601 aaaatcattg ttacaaccat tgaaaaacca tgctggacgt aacaacaacg gccgcatcac
    21661 tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc gtgatcgact tcaaacgtaa
    21721 caaagataac gttgttgcaa ctgttcaaac gatcgaatat gatccaaacc gttcagctaa
    21781 catcgcgtta gtacactatg aagatggagt gaaagcttac atcttagcac ctaaaggact
    21841 agaagtgggt atgaaacttg tttcaggtcc agaagcagat atcaaagtcg gaaatgctct
    21901 tccattagaa aacattccag ttggtactgt tatccacaac attgaaatga aacctggtaa
    21961 aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa gtacttggta aagaaggcaa
    22021 atacgtattg atccgcttga actctggtga agttcgtatg atcttagctg catgccgtgc
    22081 tacaatcggt tctgtaggta acgaacaaca cgaattaatc aacatcggta aagctggacg
    22141 ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc gtaatgaacc ctaatgatca
    22201 cccacacggt ggtggtgaag gtaaagctcc tatcggacgt aaagcaccag tttctccttg
    22261 gggtcaacca gctcttggtt acaaaactcg taacaaaaaa gctaaatcag acaaacttat
    22321 tgttcgtcgt cgcaaaaata aataattact ttgaccatgt gtcgcacatt ttgagaggag
    22381 gttcaccatg ggtcgtagtt taaagaaagg accttttgtc gatgagcatt taatgaaaaa
    22441 agttgaagct caagcagggg ttgaaaagaa aaaagttatc aaaacttggt cacgccgttc
    22501 aacagttttc ccaagttttg ttggatatac catcgcagtt tatgatggac gtaaacatgt
    22561 accagtttac atccaagaag acatggtagg acacaaatta ggtgaatttg cacctacaag
    22621 aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa cgctaatttg aggggaggat
    22681 aaatccaaat ggcagaacaa atcacatcag ctaaagcaac tgcaaaaaca gttcgcactt
    22741 cacctcgaaa agcccgttta gtaattgacc ttatcagagg taaaagtgtc gcagatgcaa
    22801 tttcaatctt gaaattcaca ccaaacaaat ctgctggaat cattgaaaaa gtattgatgt
    22861 cagcagttgc gaatgcagaa aataactttg acttagatgt tgaaaacttg gtagtaagcg
    22921 aagcttttgt taacgaagga cctacaatga aacgtttccg tcctcgtgca aaaggatcag
    22981 cttctccaat caacaaacgt acaagtcata tcacggtagt cgtatcagaa aaataaggag
    23041 ggataatcag tgggtcaaaa agtacatcca attggaatgc gtgtaggcat catccgcgac
    23101 tgggatgcta aatggtatgc tgaaaaagag tatgctgagt tcttacacga agatttaaga
    23161 atccgtaaat ttatttcaac taaacttgct gatgctgctg tatcaacagt tgaaatcgaa
    23221 cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta aaccaggtat ggttattggt
    23281 aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa acaaattaac tggtaaaaga
    23341 atccatatca atatcgtgga aatcaaaaaa ccagatctag atgcaaaatt agtaggtgaa
    23401 ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc gcgctcaaaa acaagccatc
    23461 caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc aagtatctgg tcgtctaaac
    23521 ggagcagata tcgctcgttc agaaggatac tcagaaggaa ctgttcctct tcatacttta
    23581 cgtgcggaca ttgattacgc atgggaagaa gcagatacaa catacggaaa actaggagtt
    23641 aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa aaaacactga gaaaggaggg
    23701 aaataatcat gttagtacct aaacgtgtaa aacaccgtcg tgaattccgc ggaaaaatgc
    23761 gcggggaagc taaaggcgga aaagaagtag cattcggtga atacggtttg caagctgttg
    23821 attcacattg gatcacaaac cgccaaatcg aagctgctcg tatcgcaatg actcgttaca
    23881 tgaaacgtgg tgggaaagta tggattaaaa ttttccctca caaatcttat actgccaaag
    23941 caattggggt acgtatgggt tctggtaaag gggcacctga aggatgggtt gcaccagtaa
    24001 aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga agaagtagct cgtgaagcgt
    24061 tacgtctagc ttctcacaaa ttaccaatga aaactaagat cgtaaaacgt gaggaaatgg
    24121 gtggtgaatc gaatgaaggt taaagaaatc agagaattaa ccactgccga aatgcttgat
    24181 caagaaaaac aattaaaaga agaattgttt aatcttagat tccaattagc aacaggtcaa
    24241 ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga ttgcacgcat caaaactgtg
    24301 ttgcgtgaac aagctaagta attatggaag gaggccatta agcgtatgac tgaaggaaga
    24361 aatcaacgca aagtttatca aggtcgcgtg gtttcagaca aaatggataa aacaattacc
    24421 gttgctgtcg aaacaaagaa aaaccaccct atctatggca aacgcatgaa ctattctaag
    24481 aaatacaaag tgcatgatga aaacaacaca gcaaaagttg gcgacatcgt gagaattatg
    24541 gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt tggaagtagt cgaagaagca
    24601 gttattatct aataacacga gattttccta tatagattga atgcaaaatc tgaaaggagg
    24661 atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg ctgataactc aggcgcacgt
    24721 gaaattttga cgatcaaagt ccttggtgga tctggtcgta aaactgctaa catcggtgat
    24781 gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg ttgtcaaaaa aggtgacgtc
    24841 gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc gtacagacgg ttcatatatt
    24901 aaatttgatg aaaatgctgc agtgattatt cgtgatgata aaagccctcg tggaacacgt
    24961 atctttgggc cagttgcacg tgaactacgt gaaaacaact tcatgaagat cgtttcacta
    25021 gcaccagaag ttttataatc ttgagacact tatcaaagga ggtgcgaaac agttatgttt
    25081 gttaaaaaag gcgataaagt taaagttatc actggaaaag acaaaaacaa agaaggcgtt
    25141 gtactagcag cgttcccgaa aaaagataaa gttattgtcg aaggtgtaaa tgtcgttaaa
    25201 aaacaccaaa aaccttctca agctgctcct caaggtggaa ttgttgagat ggaagcgcca
    25261 attcatgttt ctaacgtgat ggtggttgac tctactggtg tagctggccg agttggctac
    25321 aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa ccggtgaagt tttagataaa
    25381 taaaagttta aggaaggagg gtcttactga atgaaccgcc taaaagaaaa atatcttaaa
    25441 gaagtaactc catcattgat ggaaaaattt aactatagct ctgttatgca aacacctaaa
    25501 gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg tatcaaacgc taaaaactta
    25561 gataaagcag ttgaagaatt aactttgatc actggtcaaa aaccaatgat tacaaaagct
    25621 aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc caattggtgc aaaagttaca
    25681 ttgcgcggag aaagaatgta cgaattctta gacaaattgg tttctgtttc actaccacgt
    25741 gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg gtcgtggaaa ctatacttta
    25801 ggtattaaag aacaattgat cttcccagaa gtagattatg atttagtaga taaagtacgt
    25861 ggtatggaca tcgttattgt aacaacagca aactctgatg aagaatctcg tgagttgttg
    25921 acacaattag gcatgccatt ccaaaaataa ttaaaggagg cgaactacgt ggctaaaaaa
    25981 tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa cacaagctta tactcgttgc
    26041 gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc atctttgccg tatttgcttc
    26101 cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga aagctagctg gtaaaaagta
    26161 aattcgcata agaaggaggt aaaagatcaa tggtcatgac agatccaatt gcagattttc
    26221 taactcgtat ccgtaacgca aacatggtta aacatgagtc tttagaagta cctgcatcaa
    26281 aaattaaacg tgacatcgct gaaatcttga aacgtgaagg tttcgttcgt gatgttgaat
    26341 acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct aaaatttggt aaaaacggcg
    26401 aacgtgttat cacaaactta aaacgtatct ctaaaccagg tttgcgtgct tatgtcaaag
    26461 ctgacgaagt accaaaagta ttgaacggtt taggtatcgc aatcatttcg acttctgaag
    26521 gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg cggcgaagtt atcgcttacg
    26581 tatggtaatc aagtagaatc tacaaggagg tgtctttaag tgagccgtat cggaaataaa
    26641 gtcgttgtta tccctgaagg agtaacagtt actcaagacg gaaacaacgt tacagttaaa
    26701 ggaccaaaag gtgaattgac tcgtactttt tcagcagata tcaaaatgaa tatcgaagga
    26761 aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa tgaaaacaat tcatggaaca
    26821 actcgtgcga acttcaacaa catggttgta ggtgtcagcg aaggtttcca aaaagcgtta
    26881 gaactaatcg gggttgggta ccgtgcacaa ttacaaggat caaaacttgt attgaacgtt
    26941 ggttactcac atccagtaga aatcacacca ccagctggag taactgttga agtaccagct
    27001 aacacacaag taattgttaa aggtgcgaac aaagaagaag ttggcgaatt agctgcaaac
    27061 attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag gtattcgtta tgttggcgaa
    27121 tatgtacgcc gtaaagaagg taaaactggt aaataatcgt ttagaaaaat tgcatagaag
    27181 ccagcagcag gaaaaaggca tttggctgaa gtttaccttc aaaaaaatac atttttactt
    27241 gtgccgcagc ttctattttt cgaaacgtat ttactgcttg aaatagtagt acaataaaaa
    27301 taaagaggtg acaattgtga ttacaaaacc agataagaac aaaacacgtc aaaagagaca
    27361 tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc ccacgcttga acgtttttcg
    27421 ttctaacaaa aacatctacg ctcaaattat tgatgacgta gcgggtgtaa cgctagcaag
    27481 tgcctctacc ttggataaag aaatttcagg tggaacaaaa acagaagcag cacaagctgt
    27541 cggtaaacta gttgctgaac gtgcagctga aaaaggcatt aaagtagtag tctttgaccg
    27601 tggtggatac ctttaccatg gccgtgtgca agctttagct gaagctgctc gcgaaaatgg
    27661 actagaattt tagaagaagg aggaatacca ttcatggttt atatcgatcc aaaacatttg
    27721 gaattagaag accgcgttgt tgcaattaac cgtgtaacaa aagttgttaa aggtggacgt
    27781 cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa acggacacgt agggtttggt
    27841 acaggtaaag ctcaagaagt tcctgaagca atccgtaaag caattgaaga tgcgaagaaa
    27901 aacttagttg aagtacctat ggttggttca acaatcccac acgaagtaat cggtgctttt
    27961 agtggtggta gaatcctaat gaaacctgct gtagaaggtt ctggggttgc tgctggtgga
    28021 ccagttcgtg ccgtattaga attagctggg gtagcagata tcacttctaa atctttaggc
    28081 tcaaatacac caatcaacgt tgttcgcgca acagttgaag gtttgaaaca attgaaacgt
    28141 gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag aattaatcgg ataaggagga
    28201 caacaataat ggctgaatta aaagttactt taaaacgcag tattatcgga cgtcctcaaa
    28261 accaacgcga tacagttaaa gcgttaggtc ttacaaagat taacagttca gtggttaagc
    28321 cttctaatga agcaattaaa ggcatgatca atactgtttc tcacctagtg gacgttgaag
    28381 aaatttaatt tagaaaaatc ataactagaa cattaaggag gtgccaaacc tatgaaactt
    28441 catgaattaa aacctgctga aggttcacgc caagtacgta accgtgttgg tcgtggtact
    28501 tcatctggta acggaaaaac tgccggacgc ggacaaaaag gacaaaaagc tcgttcaggc
    28561 ggcggtgtgc gtttaggatt tgaaggggga caaacaccat tgttccgtcg tttaccaaaa
    28621 cgaggattca caaacgtgaa ccgtaaagac tatgcagtca ttaatcttga tgtattgaac
    28681 cgctttgaag acggagctga agtaactcct gttgctttag ttgaagctgg aatcgtgaaa
    28741 aacgaaaaag ctggaatcaa agtattagct aatggggaac taaacaagaa attaactgtg
    28801 aaagcagcaa aattctctaa agcagctcaa gaagcaattg aagcggctgg tggatcaatc
    28861 gaggtgatct aatgtttaga ctattaaaag atgcttttaa agtaaaagac attagatcga
    28921 aaatcctatt tactgtattt gtgttgtttg tatttcgcct aggtgcacat attactgtac
    28981 caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt gccatttttc aacatgttga
    29041 acttggtaag tggtagtgcg atgcaaaact tttccatttt ctcaatgggg gtttcaccat
    29101 acattactgc ttctatcgtc attcagcttc ttcaaatgga catcgtgcct cgatttgttg
    29161 aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa tcaagcgact cgatatctga
    29221 cattagtatt ggcatttatc caatctgtcg gtatcactgc tggattcaat caatacgctc
    29281 aattaggttt cgtgaataat cctaacgtag caacttacgt aacgatcggt ttgattctaa
    29341 cagccggtac gatgtttgtt acttggctag gagaacagat cacagaaaaa ggtatcggaa
    29401 atggcgtatc aatgatcatt tttgcaggta tcatttctag attgcctgaa agtatcagag
    29461 aaatttatga agattacttt ataaatattg atccttcaga catatggaaa tctgctattt
    29521 ttgtggccat tttactagta gcggtcttgt tgatcgtgac attcgtaaca tttttccaac
    29581 aagctgaacg caagatcccg atccaatata cgaaacgcgt agctggcgcg cctacaagca
    29641 gctatctgcc gttgaaagta aatgcagctg gagttattcc agttatcttt gctagttcgt
    29701 ttattgctac gccaaatgct gttttacaag cattgagcgg tagattcggt tctgaaaact
    29761 ggtacaaagt gatgatggag atctttaact ataatacagc acctggtgct acgatttata
    29821 cgattctgat cgttgccttc actttcttct acgcctttgt tcaagtaaac cctgagaaat
    29881 tagcggaaaa cttgcaaaag caaggaagtt atataccaag cgtacgccct ggtaagggga
    29941 ctgaagagta tgtctcaaga ttactgatgc gtttaagtac agttggtgca gtcttccttg
    30001 gtttagtggc attgcttcca atcattgctc aaatggtttg ggatcttcca caatcaatcg
    30061 gattaggcgg tacaagctta ctgatcgtta tcggtgtagc tttagaaact gcgaaacagt
    30121 tagaaggctt aatgctgaaa cgtaaatatg ttggatttat tgagtaagaa agcgtgttga
    30181 ggtttcccct caacacacgt tctcacacat ttattaggag ggaaaacaat gaacctcatt
    30241 ttaatggggc tgccaggcgc aggtaaagga acacaagctg aaaaaatcat tgatgcttat
    30301 ggtattccgc acatctcaac aggagatatg ttccgcgcag cgatgcaaaa cgaaacagct
    30361 cttggcctgg aagcaaagtc ttatatggat aaaggtgcat tagttcctga tgaagtaaca
    30421 aatggaatcg tgaaagagcg actagctgaa ccagatacag agaaaggctt cttattagat
    30481 ggctttccac gtacgctaga tcaagcaaaa gctctggacg caatgctaaa agatttgaat
    30541 aaaaaaattg atgctgtcat cgatatccat gttggtgaag aaatcttggt cgaacgtcta
    30601 gccggccgat ttatctgcag caattgtgga gcaacttacc ataaaatttt caaccctaca
    30661 aaagttgaag acacatgtga tcgttgcggt gggcatgaat tctatcaaag agaagatgat
    30721 aaacctgaga cggttaaaaa tcgtctggct atcaacatca aaaatagtga accaatcttg
    30781 gcttattata aagaacaagg tttgctaaac actattgatg gtgctcgtga aatcgatgct
    30841 gtatttgcag atgtacaaaa aatcattgaa aaatagcacg attgcccgta gccgacttgc
    30901 attatgcagt tactcttgtc aagttcaata aattatgata gaatatgtca gtccgacttc
    30961 tagagaatcc tctagatgat ccattttgag aattttgagg tgggaactgc agtttccgcc
    31021 attttatcgt gtgaaaatgc gaaacaagta caaggaggta ctgtgcgtgg caaaagaaga
    31081 tatgattgaa gtcgaaggta cagtcgtcga aactttgccg aatgcaatgt ttaaagttga
    31141 actagaaaac ggacaccaag ttcttgctac tgtttcaggt aaaattcgta tgcattacat
    31201 ccgtatctta cctggtgaca aagtaacggt tgaattgtct ccatacgatc ttaaccgtgg
    31261 ccgtattact tatcgcttta aataattgta ctccgtaaaa ctacaggagg tattatcatg
    31321 aaagtaagac catcagtaaa accaatgtgc gaacattgta aagtaattcg tcgtaaagga
    31381 cgcgttatgg tgatttgccc agcaaatcca aaacataaac aacgtcaagg ataattggag
    31441 gtgctataaa tatggctcgt attgcaggag tagatattcc tcgtgataaa cgtgtagtag
    31501 tttcacttac ttatatctat ggtattggta acactactgc taaaaagatc ttagctgatg
    31561 ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa tgaacaaaca gatgctatcc
    31621 gtgcagaagt agataaatta aaagttgaag gggatcttcg tcgtgaagtg aacttgaaca
    31681 tcaaacgttt gatggaaatc ggttcttacc gtggaatccg tcatcgccgt ggattgcctg
    31741 ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa aggcccagct cgcgctatcg
    31801 ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa cttcatggca gcaaaaaaag
    31861 tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg tattgcacat atccattcta
    31921 cattcaataa cactatcatc atgatcactg atactcacgg aaatgcatta gcgtggtcat
    31981 cagctggttc attaggtttc aaaggaagca aaaaatcaac tccttttgcc gctcaaatgg
    32041 cagcagaaac tgctacaaaa gcagcaatgg aacacggatt aaagactgtt gaagtaacag
    32101 ttaaaggacc tggttctgga cgtgaagcag caattcgttc attacaagct acaggtttag
    32161 aagtgactgc aattcgtgac gtgactccag ttcctcataa tggtgcccgc cctccaaaac
    32221 gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat cagtaacgtc attgaacaag
    32281 acacttttcg ttttgaaagg ggtaaagata agaatgattg agttcgaaaa accaagaatc
    32341 gcaaaaattg atgaagaaaa agattatggc aagttcatcg ttgaacctct tgaaagaggg
    32401 tatgggacta ctttagggaa ttccctacgt cgtattttat tatcttcttt gccaggtgct
    32461 gccatcacta atattcaaat cgatggtgta ctgcatgaat tctctaccat caaaggtgtt
    32521 agagaagacg tcactcaaat catcttgaac attaaaggct tagcgctgaa aatgtatggc
    32581 caagaagaaa aaacccttga aatcgatatt accggacctg ctacagtaac tgctggcgat
    32641 attatcgttg atagtgatgt agaaattctt aacaaagata tgtacatctg tagtgtctct
    32701 gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg gacgtggtta tgttcaagca
    32761 gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc cagtcgattc aatctacaca
    32821 cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg ttggacaccg tgaagatttt
    32881 gataaattga cgatggagat ttggacagat ggttcaattg aaccgttgga agcaatgagt
    32941 ttgtctgcga aaatcatgac tgaacatttg gatatttttg tgaacttaac tgatgaagca
    33001 aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa aagaaaaaat gttggaaatg
    33061 acgatcgaag agttagactt gtctgttcgc tcttacaatt gtttgaaacg tgcaggaatc
    33121 aatactgtac aagaacttac aaataaatct gagcctgaaa tgatcaaagt ccgtaactta
    33181 ggacgtaaat cattagaaga agttaaagca aaattgcacg acttaggttt aggtttacgt
    33241 aaagaagatt aaaacctttt acgaaggagg gaaaccaacg tgagttatcg taaactagga
    33301 cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa caactgattt attaatcaac
    33361 gaacgtattg tgactactga agctcgtgcg aaagaagttc gctcaactgc agaaaaaatg
    33421 atcactttag gtaaacgcgg agatttgcat gcacgtcgtc aagctgcggc tttcgtacgt
    33481 aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg tcattgaatc agctttacaa
    33541 aaactgttca acgatattgc acctcgttac gcagaacgtc aaggcggata cacacgcatt
    33601 ttgaagacag aaccaagacg cggtgatggt gcaccaatgg ttgtcttaga acttgtctaa
    33661 aatcacacta ataacatcac tttatagatg attcttttag agtgttatga tgttggaaga
    33721 tatttttccg agtctagctc agcgctgctc ctcaagcgaa tatcgcttgg ggagtaagca
    33781 ctcatcataa agtgtttttt tataccttca attacctgtc atgacagata tgtttgaagg
    33841 cataaaaaaa cattttaagc gagaaataat tgaaaatttt gtcttttttt gatagtgtta
    33901 gtaggaactt taggatgaag aaggagtaat catggagcca attattgaat tagaaaagat
    33961 aaattataaa taccagccag atgatcttcg ccccgcattg aaagatgtct cctttacaat
    34021 tgataaaggt gagtggatcg ctattattgg acacaatggt tcaggaaaat cgacacttgc
    34081 caaaacgatc aatggattgc ttctgccaga atcagggatc gtgaaagtgg gaaatcaaat
    34141 attggatgaa gaaaatattt ggacgatccg acagatggtt ggaatggttt ttcaaaatcc
    34201 agacaatcag tttgttggtt cgacagtaga agatgacgtt gcttttggtc ttgaaaatca
    34261 agggattccc agagaagaaa tgctagtgcg tgtgaaagat gcacttgaga aagttagaat
    34321 ggctgagttt gcttcacgcg agcctgctcg tttatcagga gggcaaaaac aacgtgttgc
    34381 cattgccggc gtggttgcct tacgacctga tatcattatt ttagatgaag caaccagtat
    34441 gctagatcca gaaggtcgtg aagaagtgat ctctacgatc aagaagatca aagaagaaag
    34501 tcaattgacc gttatatcta tcactcatga tattgatgaa gcagctaatg ccaatcggat
    34561 actagtgatg agacaaggcg aacttgtccg tgaaggtaca ccaaaagaaa ttttttctgc
    34621 tggtcctgaa ctgatcgact taggtctaga tctaccattt cctgaaaaac tgaagagcgc
    34681 actgaaagaa cgtggggttg atgtgcccag tgaatatatg acagaagaaa ggatggtgga
    34741 ttggttatgg acatccgttt tgaacaagta gacttcactt atcagccgaa tacaccattt
    34801 gaacaaagag ccttatttga catcaatatg acgatcaaag aaaacagtta tactgcatta
    34861 gtagggcata ccggaagcgg gaaatcaacc ttacttcagc atttgaatgc gcttgttaag
    34921 ccaacaagcg gtacagtcca tattggagaa cgagatattc agccggatac ggataataag
    34981 aatttaaagc ccatccgaaa aaaagttggc atcgtctttc agtttccaga agcacagcta
    35041 ttcgaagaaa cggtagcaaa agacattgct ttcggtccta aaaactttgg tgtcagcgaa
    35101 gaagaagcac tagtcctagc aaaagaaaca ttagaacaag ttgggctgga tgaaagctat
    35161 ttggaacgtt cgccatttga actttcagga gggcaaatgc gtagagtagc aatcgctggt
    35221 gtgcttgcca tgaggccgga agtacttgta ttggatgaac caacggcagg acttgatccc
    35281 cagggcagaa aagaaatgat ggagatgttt tggcgtcttc ataaggaaca gcagatcact
    35341 attgtattag ttacgcattt aatggatgat gtagccaatt ttgcagatta tgtctatgtg
    35401 ttggaaaagg gacgaatcgt taatagcggg gagccgcaag aggtctttca aaacattgaa
    35461 tggctaaaag aaaaacaact aggcgtacct actgccaccg agtttgcaga agaattaatg
    35521 gctaaaggga tgaactttgc taccttgcct ttgacagctg aagaattagc agacgctatc
    35581 gtccattatg ctggaggaaa tgcccatgat gaataaattg atatttggtc gttatatgcc
    35641 tggagattcc ttgatccatc gattggatcc ccgagctaaa ttattagcca gtttttattt
    35701 tatcggaatc atttttttag caaataattg ggaaacgtac cttttgttgg catttttcac
    35761 attactttct gtcttcttgt caaaagtaga tttgaaattt tttatccgag gcattcgccc
    35821 attgatctgg ctaatcctct tcactgtagc tttgcaggtc ttgtttacaa gtggcggaga
    35881 aacttattgg agttggggtg ttttccatat cactgaattt ggtgtgatga acggcctctt
    35941 tattttctgt cgattcgtgt tgatcatttt tatgtcgact ctactcacgc ttactacacc
    36001 gcctttggaa ttatcggatg cgattgagta cttgcttcgt ccattgaaag ccgtacggtt
    36061 cccagtccac gaaatttcat tgatgctgtc gattgctctt cgttttgtgc ctacattgat
    36121 ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc ggtgttgatt ttggtgaagg
    36181 aaacctagtt cagaaaatga aagcagtcat tcctttgctg attcctttat ttgtcagcag
    36241 cttcaaccgt gcggaagatc ttgcgactgc tatggaagca agaggctaca aaggtgggga
    36301 aggaagaacg aaatatcgtg tattgcattg gcataataga gacacgatgg ttatcattgc
    36361 ttttgtatta ttaactgtgc tactgatctt tttaagagga taaacgttca gtgggccgct
    36421 atctattctt ttcttgctga tctgtttgca agaaaggatg caagcagtgt atgacaagaa
    36481 atattagggt ctgtggcaga ctaaggtcac aggcctcttt cttttatctg aaaagaaaga
    36541 acgaaaaaat tttatagaaa aat
//
LOCUS       DADXTH010000023        33288 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.023, whole genome shotgun sequence.
ACCESSION   DADXTH010000023 DADXTH010000000
VERSION     DADXTH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.023"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            134..409
                     /locus_tag="IWI97_001096"
     CDS             134..409
                     /locus_tag="IWI97_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBC2462616.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="IWI97_001097"
     CDS             415..1182
                     /locus_tag="IWI97_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBC2462617.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="IWI97_001098"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="IWI97_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBC2462618.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="IWI97_001099"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="IWI97_001099"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBC2462619.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="IWI97_001100"
     CDS             5326..5628
                     /locus_tag="IWI97_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBC2462620.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="IWI97_001101"
     CDS             5631..6794
                     /locus_tag="IWI97_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBC2462621.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="IWI97_001102"
     CDS             6820..10248
                     /locus_tag="IWI97_001102"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBC2462622.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="IWI97_001103"
     CDS             10269..11393
                     /locus_tag="IWI97_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462623.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="IWI97_001104"
     CDS             11383..11820
                     /locus_tag="IWI97_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBC2462624.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="IWI97_001105"
     CDS             11804..12142
                     /locus_tag="IWI97_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBC2462625.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="IWI97_001106"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="IWI97_001106"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBC2462626.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="IWI97_001107"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="IWI97_001107"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBC2462627.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="IWI97_001108"
     CDS             13299..14342
                     /locus_tag="IWI97_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBC2462628.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="IWI97_001109"
     CDS             14501..15229
                     /locus_tag="IWI97_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBC2462629.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="IWI97_001110"
     CDS             15345..15590
                     /locus_tag="IWI97_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBC2462630.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="IWI97_001111"
     CDS             15587..15805
                     /locus_tag="IWI97_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBC2462631.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="IWI97_001112"
     CDS             15941..16480
                     /locus_tag="IWI97_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBC2462632.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="IWI97_001113"
     CDS             16473..18746
                     /locus_tag="IWI97_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBC2462633.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="IWI97_001114"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="IWI97_001114"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBC2462634.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="IWI97_001115"
     CDS             complement(19919..20173)
                     /locus_tag="IWI97_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBC2462635.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20573..20842)
                     /locus_tag="IWI97_001116"
     CDS             complement(20573..20842)
                     /locus_tag="IWI97_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462636.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21312..21767)
                     /locus_tag="IWI97_001117"
     CDS             complement(21312..21767)
                     /locus_tag="IWI97_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBC2462637.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21905..23833)
                     /locus_tag="IWI97_001118"
     CDS             complement(21905..23833)
                     /locus_tag="IWI97_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBC2462638.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24030..24863)
                     /locus_tag="IWI97_001119"
     CDS             complement(24030..24863)
                     /locus_tag="IWI97_001119"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBC2462639.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25055..25759)
                     /locus_tag="IWI97_001120"
     CDS             complement(25055..25759)
                     /locus_tag="IWI97_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBC2462640.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGAIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25782..26408)
                     /locus_tag="IWI97_001121"
     CDS             complement(25782..26408)
                     /locus_tag="IWI97_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBC2462641.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26734..29109
                     /locus_tag="IWI97_001122"
     CDS             26734..29109
                     /locus_tag="IWI97_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBC2462642.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29133..29624)
                     /locus_tag="IWI97_001123"
     CDS             complement(29133..29624)
                     /locus_tag="IWI97_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBC2462643.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29811..30731
                     /locus_tag="IWI97_001124"
     CDS             29811..30731
                     /locus_tag="IWI97_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2462644.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30885..31229
                     /locus_tag="IWI97_001125"
     CDS             30885..31229
                     /locus_tag="IWI97_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBC2462645.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31419..31919)
                     /locus_tag="IWI97_001126"
     CDS             complement(31419..31919)
                     /locus_tag="IWI97_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2462646.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32447..32530
                     /locus_tag="IWI97_001127"
     tRNA            32447..32530
                     /locus_tag="IWI97_001127"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32481..32483,aa:Leu,seq:cag)
     gene            complement(32854..>33288)
                     /locus_tag="IWI97_001128"
     CDS             complement(32854..>33288)
                     /locus_tag="IWI97_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBC2462647.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11009 a         5771 c         6784 g         9724 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga ggaattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagatg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccatgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta tataacaaag
    20461 agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt
    20521 ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa ggctattctg
    20581 gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt tttatgacca
    20641 actggttccg acggttccat ttgccactta cttccagcac atatttattt aggggcaaac
    20701 gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca cggtcctcaa
    20761 gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac gtatcaatta
    20821 aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag
    20881 gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt tcttttgtcc
    20941 actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag agcatcgaat
    21001 tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt aaaaaaagcc
    21061 cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa gcagtatcaa
    21121 acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc aaaagtaccg
    21181 ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac attgtatcac
    21241 ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc ctaaaataga
    21301 caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt gataccagaa
    21361 gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat cgtgccgaaa
    21421 acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt ggaagataag
    21481 acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt aaaggcttct
    21541 aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc cgctgtgatg
    21601 atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga acgggaggct
    21661 ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg atttttagct
    21721 aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa atatattcct
    21781 cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag gaattcatcc
    21841 ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata aaaaaacact
    21901 ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta catttttttg
    21961 aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga ataaccaata
    22021 ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc aggaagttag
    22081 aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg atgatccgcc
    22141 gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt ttcattaaga
    22201 cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca cctaaagaag
    22261 cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt acttggtaaa
    22321 gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt gcatagtaat
    22381 gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg ccggttatca
    22441 tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta tttgcttttg
    22501 gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga taaatcacgg
    22561 ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg attatcatcg
    22621 tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag gtagtttgta
    22681 atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt attgtgcaat
    22741 cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa
    22801 gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca actggttttc
    22861 ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa agcttttcta
    22921 aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga gcagctagag
    22981 aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat aataccattc
    23041 ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga aggatcatta
    23101 gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg ataaatgaag
    23161 ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta gcaaagacag
    23221 tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc agcgttcctg
    23281 catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt aatgcagact
    23341 catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct gctcctttag
    23401 cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc atttgtccat
    23461 tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta atttcttttt
    23521 gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg aatgtcaaca
    23581 ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa gccatcaata
    23641 aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat gtttgtttat
    23701 atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga gagaatcggt
    23761 cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca aaagcttttt
    23821 tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata ataaaaatgt
    23881 aattgataat aataacagct aaatgagaac gataatggtt atcatttggt gattaagctt
    23941 gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac cagaacattt
    24001 ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata accaatcaaa
    24061 atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt cttcaaatag
    24121 cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt tcctacaaga
    24181 agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt gacgatcttc
    24241 aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca
    24301 aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg ggatgttccg
    24361 ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta cgtaaagtca
    24421 ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat aataaaggca
    24481 ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt tgcttctaac
    24541 caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat cactttaaac
    24601 caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa tgggtttaac
    24661 ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac gacattgaac
    24721 atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag gatcaaatga
    24781 ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag aatcactgct
    24841 ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac atcttatagt
    24901 ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa cttgagaaat
    24961 aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag actttgcctt
    25021 ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg cgactatgtg
    25081 ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat cgaccgtttg
    25141 cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc tgtttaggat
    25201 cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa acatttccac
    25261 caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca cggtctcgca
    25321 atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca ttagcaattt
    25381 gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc aatacacgag
    25441 ggatcgcacc ccacatttcc atgtcatatc caccttcaag agacccgaaa caccattctc
    25501 taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct cctagaataa
    25561 tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa aagtcgacat
    25621 tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg aggggtgtat
    25681 cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt cgtacgatat
    25741 aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt gaaaaaagga
    25801 tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata gactgcaaat
    25861 tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc taaattccct
    25921 gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga aacgacaaaa
    25981 tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac gacgcgaaaa
    26041 gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg cgagacaata
    26101 acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg atcacctagc
    26161 catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg taaaggttcc
    26221 actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg ctgttggttt
    26281 gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat gatcaaaagt
    26341 gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt taatctttcg
    26401 atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag tataacgcat
    26461 tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa ttatcagact
    26521 atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata gcattttaat
    26581 atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat atccctgcct
    26641 aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa ctaggttttt
    26701 tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa aatatctgtg
    26761 cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa aaaaagtaac
    26821 catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc gggagaaaac
    26881 tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta cctgatcgtt
    26941 gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga tgaaatcaaa
    27001 acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt ctttcatcaa
    27061 gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat aaatctgcaa
    27121 ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca tcagacaaga
    27181 gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg gcttgttttt
    27241 caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt cccccatgat
    27301 gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat ccgtacgaaa
    27361 cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac gttattcccc
    27421 gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac ggctaaaaca
    27481 ataactagac aagtagctga agatgctatg acagcattaa aagatacagg agctgaagta
    27541 aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac gacctgtaat
    27601 ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg tttatgggat
    27661 cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc aagaaagcat
    27721 acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt aatcattggg
    27781 gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga agatgaaaaa
    27841 aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg ttcccgagag
    27901 atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc tataccaaag
    27961 gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga ttcgatccga
    28021 gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc tgaatcttta
    28081 gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga atttcctgag
    28141 cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt tttgaaggtc
    28201 agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg cgatctgata
    28261 gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga taaaggaaaa
    28321 agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga aacactgggg
    28381 ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa ccagcaagtt
    28441 ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct ggcaaaatta
    28501 gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt tttctttcct
    28561 tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc cgacagcaat
    28621 gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt aacaaaattt
    28681 caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat tttttcagaa
    28741 ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt aggtttgccg
    28801 cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga acaccttgga
    28861 tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg tggtcgagtg
    28921 attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt ttctagtttt
    28981 tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg aagtgttggg
    29041 gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa tcaaaaaaac
    29101 gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa ttgcaactcg
    29161 attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc acggttctta
    29221 cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt ttcatttttg
    29281 atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa atcggcagtt
    29341 tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt tttgattggc
    29401 acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat tttgaaatca
    29461 tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt agaatccttg
    29521 aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt tacttcttct
    29581 gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac cctcctgata
    29641 tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga ttcttacctg
    29701 tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat aaactgttat
    29761 tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt atgactgcgt
    29821 atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga ttgaccgctg
    29881 cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg atgaatggga
    29941 tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata gacacaggaa
    30001 catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa gtaggaaaac
    30061 gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg atgctgcttc
    30121 cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt gttttattag
    30181 gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg gatatcatct
    30241 ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg attctaaatg
    30301 gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg tatacgatta
    30361 tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat caaaaagtaa
    30421 cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca aaacggatgg
    30481 tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa gggaacatga
    30541 tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt catgaaatcg
    30601 acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg tttgcaaatg
    30661 aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa aatcgttcac
    30721 aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca tctaataaga
    30781 acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca agcagctaaa
    30841 aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc gaagcaaacc
    30901 aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct gccttggaaa
    30961 tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt tatgatatag
    31021 aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact atgggctgtc
    31081 cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg ccagaagtaa
    31141 cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag atgagccgtt
    31201 atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat gagaagcaaa
    31261 taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa ggtttctttg
    31321 tttataaaat tttgtctgct atattataag ttaattaaat aaatgtgttc tattgataaa
    31381 ggacattcaa atccctttcc ttttggaatc cttctttttt acatcagttt gattttcgta
    31441 tagtattctt tctcatcttc acgataaatc gcagccccat ttttttgttg tgctttaaga
    31501 ctgccaagat tttttttatt tacagacaaa agtgcctcat caatgccgat tttttttgcc
    31561 tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt ttttacgata attaggggag
    31621 atgccgtaac caatatggcc agcgccgttc cttaatgttt cgttcagttt atggcgcaaa
    31681 ttaaaaattc ctacatactc atgttcatca ctttctaaaa taaagacggt tgcgggaacg
    31741 taactctcag gaagatcgat tcctaaagac gttttttcgc atttttttac ataatctaaa
    31801 aattcagcaa atgtatagcc ataagcattg ttgataaatc cactctcatt tggtccaaag
    31861 ctagtttgta gctgataagc tttctttaaa tctgcttccc atagtttgat cagtttcatc
    31921 gtattcacct ctttaagtaa ccttattcat ttgttttcta ctatagcact attgttgtaa
    31981 atagccatgc ctatttttat ttattgtgtc atatcgtatt aagaatcatc gaacaataca
    32041 aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt atgatctcta
    32101 gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag taatcaacag
    32161 aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag aggaaaaaag
    32221 aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa cattgaaatc
    32281 gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga gttgctcgga
    32341 tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag aacaaaaata
    32401 tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg ctatggcgga
    32461 attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag ttcgagtctt
    32521 cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga tgagaaagaa
    32581 aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc agattcaaat
    32641 aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat tgattcaaat
    32701 agtagattta tagattctga tacaagaaat actggcaaac gatagttaag gaataaagac
    32761 taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct cttactatat
    32821 tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg cttcccagaa
    32881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    32941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacggccgat
    33001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    33061 tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    33121 ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa caggttgttc
    33181 agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    33241 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DADXTH010000024        32467 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.024, whole genome shotgun sequence.
ACCESSION   DADXTH010000024 DADXTH010000000
VERSION     DADXTH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.024"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            45..1943
                     /locus_tag="IWI97_001129"
     CDS             45..1943
                     /locus_tag="IWI97_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBC2462648.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2242..2961)
                     /locus_tag="IWI97_001130"
     CDS             complement(2242..2961)
                     /locus_tag="IWI97_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBC2462649.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3298..3630
                     /locus_tag="IWI97_001131"
     CDS             3298..3630
                     /locus_tag="IWI97_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462650.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3617..5620
                     /locus_tag="IWI97_001132"
     CDS             3617..5620
                     /locus_tag="IWI97_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBC2462651.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5639..6109
                     /locus_tag="IWI97_001133"
     CDS             5639..6109
                     /locus_tag="IWI97_001133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBC2462652.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6130..6714
                     /locus_tag="IWI97_001134"
     CDS             6130..6714
                     /locus_tag="IWI97_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBC2462653.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6729..7715
                     /locus_tag="IWI97_001135"
     CDS             6729..7715
                     /locus_tag="IWI97_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBC2462654.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7705..8016
                     /locus_tag="IWI97_001136"
     CDS             7705..8016
                     /locus_tag="IWI97_001136"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBC2462655.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8038..9819
                     /locus_tag="IWI97_001137"
     CDS             8038..9819
                     /locus_tag="IWI97_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBC2462656.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9812..11188
                     /locus_tag="IWI97_001138"
     CDS             9812..11188
                     /locus_tag="IWI97_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBC2462657.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGQPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11195..11830
                     /locus_tag="IWI97_001139"
     CDS             11195..11830
                     /locus_tag="IWI97_001139"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBC2462658.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11860..13215
                     /locus_tag="IWI97_001140"
     CDS             11860..13215
                     /locus_tag="IWI97_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBC2462659.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13407..14801)
                     /locus_tag="IWI97_001141"
     CDS             complement(13407..14801)
                     /locus_tag="IWI97_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBC2462660.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14893..15864
                     /locus_tag="IWI97_001142"
     CDS             14893..15864
                     /locus_tag="IWI97_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBC2462661.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15861..16643
                     /locus_tag="IWI97_001143"
     CDS             15861..16643
                     /locus_tag="IWI97_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBC2462662.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16812..17183)
                     /locus_tag="IWI97_001144"
     CDS             complement(16812..17183)
                     /locus_tag="IWI97_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2462663.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17317..17760
                     /locus_tag="IWI97_001145"
     CDS             17317..17760
                     /locus_tag="IWI97_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBC2462664.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17803..18612)
                     /gene="yidA"
                     /locus_tag="IWI97_001146"
     CDS             complement(17803..18612)
                     /gene="yidA"
                     /locus_tag="IWI97_001146"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBC2462665.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18631..19998)
                     /locus_tag="IWI97_001147"
     CDS             complement(18631..19998)
                     /locus_tag="IWI97_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2462666.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19982..20812)
                     /locus_tag="IWI97_001148"
     CDS             complement(19982..20812)
                     /locus_tag="IWI97_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBC2462667.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20975..21412
                     /locus_tag="IWI97_001149"
     CDS             20975..21412
                     /locus_tag="IWI97_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBC2462668.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21488..22108
                     /locus_tag="IWI97_001150"
     CDS             21488..22108
                     /locus_tag="IWI97_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBC2462669.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22297..22803
                     /locus_tag="IWI97_001151"
     CDS             22297..22803
                     /locus_tag="IWI97_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBC2462670.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22820..23719
                     /locus_tag="IWI97_001152"
     CDS             22820..23719
                     /locus_tag="IWI97_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBC2462671.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23806..24690
                     /locus_tag="IWI97_001153"
     CDS             23806..24690
                     /locus_tag="IWI97_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBC2462672.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24882..24953)
                     /locus_tag="IWI97_001154"
     tRNA            complement(24882..24953)
                     /locus_tag="IWI97_001154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24918..24920),aa:Arg,seq:ccg)
     gene            25013..26347
                     /gene="rpoN"
                     /locus_tag="IWI97_001155"
     CDS             25013..26347
                     /gene="rpoN"
                     /locus_tag="IWI97_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBC2462673.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26496..27587
                     /locus_tag="IWI97_001156"
     CDS             26496..27587
                     /locus_tag="IWI97_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBC2462674.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            27628..28629
                     /gene="gap"
                     /locus_tag="IWI97_001157"
     CDS             27628..28629
                     /gene="gap"
                     /locus_tag="IWI97_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBC2462675.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28739..29929
                     /locus_tag="IWI97_001158"
     CDS             28739..29929
                     /locus_tag="IWI97_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBC2462676.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30033..30788
                     /locus_tag="IWI97_001159"
     CDS             30033..30788
                     /locus_tag="IWI97_001159"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBC2462677.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            30904..32202
                     /gene="eno"
                     /locus_tag="IWI97_001160"
     CDS             30904..32202
                     /gene="eno"
                     /locus_tag="IWI97_001160"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBC2462678.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        10721 a         5747 c         6911 g         9088 t
ORIGIN      
        1 acaactttat aatgttgagt ataccaattt ttggaggcgt tttaatggac cgctatttag
       61 atttactttc agagaagttc cctacaacgg aagatgtcat cacggaaatc atcaatctcg
      121 aagcaatttt gcagcttcca aaaggaacag agttattttt gagcgatata catggcgaat
      181 ttcctgcttt tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa aagatcaaag
      241 accttttctc tgaacaaatg acggaaacgg agatcagcca attcactatt tttacggctt
      301 atccagaata tgtgttgacg actgattggt acaaaaaaca agataaatcc cagctgatcc
      361 atcgtttgat cgatctttta cggtttacga cagtcaaata cactcgatca aaagttcgaa
      421 aaggattgcc gaaagaatat agctatatca ttgaggaact cttgtatatc gatgatcgta
      481 tcaacgggaa aaaagactat gtaaaaaaaa tcattgaaca acttgtcttg atgaaggaag
      541 aagaaaaatt tttaacaaaa cttgcgcaga cgatccaaaa atctgtcatc gatcatttgc
      601 atatcgtggg tgatattttt gatcgaggaa ctcaagcagc caaagtaatg gatcgaatca
      661 tggatttttc ctctgtagat atccagtggg ggaatcatga tattttgtgg attggtgcgt
      721 attgcggctc tgaggcatgt ttattgacgc ttttacggat tgccgcacgc tacaactatt
      781 tatttgaatt agagaaagaa tatggattaa atttacgtcc attattttct tttgcccatc
      841 aaacctatca gaagaatcca gtatttaccc ctaaaaacag gaaagatcta tctgaaagag
      901 agcaagaaga attagaaaag atccatcaag cactttctat cctccaattc aaatcagaac
      961 atcagttgtt gaatcgacga cctgaattta aaatggacga tcgtaaattg ttggaaaaaa
     1021 tcaactatga ggaatcaacg atcgttttag aaggtcagct ctatgagttg aaagggactt
     1081 gttttcaaac gatccagcca gatgatccta aaaaattctt ggaagaggaa caaaaagtga
     1141 tcgatagttt gatgtattca ttccagcact cgttaagaat gcaaaaacac atgacgtttt
     1201 tgatcgaaaa agggggaatg tatctcacga acaatcagca tgtactgttc catggctgta
     1261 tacctgtaga tgaaaagggg cagctcttag catttgaatt agaccaatct tatcgcggaa
     1321 aagaactgct gggatttttt gaaaaacaga taactgaaag cgcaaaagat ttgactgcaa
     1381 atgaagattt ggcgaccgat ttgatttggt atgcgtggag cgggccattt tcgcctttat
     1441 tcggcaagag taaaatggca acttttgagc gctattttct cacagagcct catacgcacg
     1501 tcgaaaaaag caatccctat tatcacttgc gagatgaaga gtggttcagt gaaaaaattt
     1561 tggcagaatt cgatgcaaaa gaagaaggtt cagcaatcat caatggacac acgccagtaa
     1621 aagtaaaaaa aggagagtca ccgatcaaag caaacggaaa agcatttgtt attgacggag
     1681 gtctatccaa agcttatcaa aaaacaaccg ggattgctgg ctactcatta ctatgcaatt
     1741 cttacggttt ccaaatcgtg acacattatc cgttcttgcc tattgaacaa caatttgcaa
     1801 aaaaatctga tcaaacgaat gtaaaaaaag tgatcgagca gcagctccca cgcaaactta
     1861 atcggaatac gacaaaagga atggaattac agcaacagat tactcaactc caaaggctgt
     1921 tggattatcg aaaggacgat tgagaaaaat gctagccaac attgctcaat agactaaaaa
     1981 gcgactgcaa caaaaggcgt gtcgcagtct ccttcggaat tagctcaaaa aacggtaaaa
     2041 tattagaagc tatttttctg tttatttctc taatactcag gtcagggcgc tgatttcacg
     2101 acctcttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccaac
     2161 aaacatgaga agctgttttt tgaaattgct ccttgtgact cagagttgaa cggcaggctc
     2221 acgacctaat cagaaaaacg attaaaattc attcacaaag aaatcgaata aacgctgttc
     2281 agatgaatct acacgatgag aaagttccgg atgccattgg acaccaagga ttttgtgcat
     2341 attatcttta ctttggactg cttcaatgag tccatcgttg gaaaaggcga tggcttttaa
     2401 caaaggagaa agatctttga ctgcctgatg atgataggaa ttcacaaagt atgaatcagg
     2461 gagaaggtca cttaataaac tgtccgaaac gattttgact tcgtgagttg caaactgcgg
     2521 ggcagtgggc tgttgttcgt gttttacgga ccattttgga tataatgaaa gatcttgata
     2581 aagtgttccc tccaacacga cattcaatag ttgaagtcct cggcagactg caaaaatcgg
     2641 ttttctttgt ttcaatgctt ccaagatcaa tgcctgttcg aattggtctc gttgaatatt
     2701 tgtttccgtc agttttggat gagggtcttc catgtaaagt tgcggactaa catcctgacc
     2761 gcctgcaaga atgagtttgt cgattttctc aattagttga ggcgctgaat ctggagacat
     2821 gactggcaga ataagaggta atccttgagc atctaaaact gcatccacaa atccctgcgg
     2881 tgtatagctt acttgatttc cttggaaagt atctgtcgct cgaatcagtt gattcccggc
     2941 aatgccgatg attggtttca tttttattcc ccctaaagta gactaattgt aacaaggatt
     3001 taaaaaaatt taaactatgt aagacttgcc cctatactta tctgttcttg ctgtgttact
     3061 atctgtaaag agaattttcg tgtgaaattc atgacgatag ttgaaaatat tgcttaattt
     3121 gttataataa aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag gatctagttc
     3181 ttcaaggggt gagaaggaca ttttattgaa ggggcctgcc tatactaagt tatctttaga
     3241 ggtacatact ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg aaaggaagtg
     3301 aagcaagtgt tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa tgagaagcgt
     3361 gctcgaaccc tacggcagca gttgaagacc tatgcagaag aaaaagagca agaattggag
     3421 ataatccaaa cgaacagtcg gcaacagctg aaacagttgg tagaagaaaa agaacaattc
     3481 gaaaagcaag aattagaaaa agaaaaaatg aaattgaaag cagaagcaga caaggtgcaa
     3541 gcaactttga aagaccaata tcaaaagcat cagagtcaag caatcggtgc aatcattgaa
     3601 agggtgaaag aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca tttcagataa
     3661 aagaaatcag gagaggattt tacaagcagt acaaggaatc caaaatgttg aaatccggga
     3721 tctgtatcaa aatagaacaa acaaccagtg ggtagaaaaa tattttccag ataccacgat
     3781 tattgataaa gaagcaaaat taagcgcttt aggcaatcgg ctgaaagaca tccaagaagc
     3841 tatccaattc atacaacacc acggcgatag ccgtcaaaag aaactccatt tgaaacgtca
     3901 agaactttcc ttacaagact tggaagcaaa ttattccgaa gaaaatttca tgaataaatt
     3961 agaagaagtc cttgatttaa aagaaaagtg ggaacagcta actgaaaaac gtgatcgttt
     4021 agaagaagag gaagaatggc ttcttcattg gcaacagctg gatattgctc caagtatgta
     4081 tgcaagtaag acgaccattt ttgagatggg gactgtgagt gcagcaaatt ttgaaagttt
     4141 caaggaagaa ctaagtaagc ttgatgacgt ttatatggaa gaaatagatt atacgccaaa
     4201 acaagtttat ctttcgtatg ttgctttaaa aaaatcagtt gctgctatag aagaaattgc
     4261 tgggaaatac gggttcaata aagaaacgta tccttatgaa caaaatccta aagaaaaatt
     4321 gcaagaaacc aaacagcaat tatccattgt tcacgagcag caaaaaaaga taacgacagc
     4381 gttaggtcag tgcagcggct atatccaaga atttgaatgg gtagaagaag tcacacttgc
     4441 cgttgcagaa cgggaaaaag taaaagaacg gtttatccat gccacttatc tgatcgtttt
     4501 gcaaggttgg gtagatgcag aagaaaaaca gaatttgatg catgtgttaa acgaaactgt
     4561 atcagaagaa gatatatacg ttagtttcga aacaccaaac tcagaagaga ttcagcagga
     4621 agtccctact aaattgaaaa accatccgct tatcgaacct tttgaattgc tgactgaaat
     4681 gtacagtctg ccaaaatatg aagaagtaga tccaacgcct tggatgacac cattttatct
     4741 tgtcttcttc gggatgatgg tcgcagatgt tggttacgga ttgctgatgc tcattgggac
     4801 aatactagct caaaaactat ttgttttacc ccgcgggatg aaacgtttcg taaaattctt
     4861 tgagattctc tcggtaccat cgatcatctg gggattgatc tacagttcat ttttcggaca
     4921 ggcattacca aaagaactat ttggtatccg cttgcctttc ccaatcctat ctacgacgga
     4981 tgacgtcaat acgattttga ttttatccgt gattttcggt ctgatacaga tcttggttgg
     5041 attattcgtg gcagcaaagg aaaatatcag gaggaaagat tacttgagtg ctgtcagtga
     5101 tggttttgcc tggcaaggga tcctgattgg gatcgttatt gcattgattg gtgtcatgtt
     5161 gataaaaaat acagcatttg tttatcttgg cgcggggcta tcgatcattg ctgctttatg
     5221 tatcttaatc gtgccgatta tccaatcgcc gtctaaagtc aagggtgctg ccaaaggagc
     5281 gtataatctt tacggattga ctggttatat cggtgattta gtcagttata cacgtttgat
     5341 ggcactaggt atttcaggcg gaagtattgg cgcggcattc aatatgctag ttgctttcat
     5401 gccgccagcc gctcgattca gtgtagggat ccttttgatc atcgctttac acgcactgaa
     5461 tatgtttttg acactgttaa gtgcctatgt ccatggtgca cgattgcagt atgtcgaatt
     5521 ttttggaaaa ttttatacag gaggcggacg tgcctttgac ccgctgaaaa cggcggaaaa
     5581 atacgtcaac atcaatcata agaaaaaaaa caataaataa ttggaggatt tttatacaat
     5641 gatggattac ttaattagtc aaaatggtgg aatggtattt gcagtattag cgatggcaac
     5701 agcgacgatt ttttcaggga ttggttcagc taaaggtgtt ggaatgacag gtgaggcagc
     5761 agcagcgtta acaacaagtc aacccgaaaa attcggacag gctttgattc tgcaattgct
     5821 tccaggtacg caagggttgt atggattcgt tatcgccttt ttgattttta tcaacttagg
     5881 aaatgatatg tcagttgtac aaggattgaa ctttctaggc gcttctctgc cgattgcttt
     5941 tacaggtttg ttttcaggta tcgcccaagg gaaagttgca gcagcaggta tccaaatttt
     6001 agcgaaaaaa cctgaacatg ctacaaaagg aatcattttt gctgcgatgg ttgaaacata
     6061 tgcgatttta ggtttcgtta tttccttcct attagtattg aatgcctaag cataaaggag
     6121 gaaaagcaga tgaacgccat tgaaaaaatc atctctcaga tgaatgaagc agcagagcaa
     6181 gaaagagcgg cgttagagca agaagagcga atgaagatcg atcaaaactt tgaacaaaaa
     6241 cgtacgcaaa tcgaaacaga acatcaaaaa caaaaagaaa aacaaatcga attacttgaa
     6301 aaaaaatacc gtcagcttag gaatcgccaa caagtagaag tacgacaaga aaatttgaat
     6361 gcgaagcagg aatttttacg acgattgttt gcagatgcaa tagctgaaat ggagaattgg
     6421 gatgaatctg aacaaatcca gtttatcaaa aattcattgt attcattacc tttgacaggc
     6481 aaagtaacat tcattgctgg agaaaaatca gcagcttatc tttctcagac attgctggat
     6541 gaatggaaca atgaacttcc ctttatgatg gttttaagtg atgaaactgt tgctgatcaa
     6601 gcgggttttc tgataaacga tcaaggagtt cagtataatt ttcttttcag cagccttgta
     6661 caagatatcc aaggaacgat gagttttgag attgccaatc aattattcga ataaggaggg
     6721 atcagtgtat ggaataccac gaactaaatc ccttgattcg cggaagagag cttgaacttt
     6781 tgtccaatga gacatttgaa cggatgattc aaacaaattc tgtcgaagca cttggtgaaa
     6841 tattgaaatc cacgatttac agtccatata tttatgacgg atatgaatat gacttcgaac
     6901 agaatctttt aaaggagcag agccgtctat ttcgctggtt aaaggaatca gcaccggagc
     6961 ctgaaatcgt ttggatctat acgatgcgct atactttcca taatctgaag gttttgacaa
     7021 aagccgagat gacaaatcaa aatctggacc atttgtatat cgacgatgga ttttattcat
     7081 tagaaacctt gaaggatgcc attcatacgc aagtttcaaa tgaactccct gaaagcatta
     7141 tggacagtat acgagaagta cgtgattatt ttgaagagtc taccattctt cagggaattg
     7201 acgtgattta tgatcgacag tttttgacag agcagcgacg gcttgccgat aagctaggat
     7261 atcaagaatt gctgacagaa gtcattgctt ttattgactt gaccaatatc acaacgatgg
     7321 ctcgaggaat attgcagaaa agaacatatg ggttcatgac gacggtactt tccagctcgg
     7381 gaagtatcga aaaagaagta tttttgaact ttgtagataa agatatggaa gattacacga
     7441 attttctatt agagacagat tatgctgatt ttctgcgccc tgtcatccgt gagggaaatc
     7501 ttgatctcgt aaagcttgaa cagttgaaag atgattatct aagttcattg tatcaagaag
     7561 cacaaaccca ggctttcgga ccactgccac tactagcctt tttgaacgca aaagaggtag
     7621 aaagcaaaaa ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa ccagaaacaa
     7681 tcagaaagcg ggtgagacaa gtctatgacg cataagatcg gtgtagttgg cgacaaagat
     7741 tctgttttgc cattcaaatt gtttggcttt gatgttcgtt atggtacaac caagcaaacc
     7801 gttcgtcagg caattgaaga aatggcaaag aatgagtatg gcgtgattta tgtcacagag
     7861 caatgtgcag cattgagccc agaaacgatc gaacgttaca aggaacaaat gatacctgct
     7921 atcgtattga ttcccaatca tcaaggatca ttagggatag ggctggaaga aattcaaaaa
     7981 aacgtagaaa aagcagtagg tcaaaatatc ttatagtatg aaaaggagat agaaattttg
     8041 caaattggaa aaatcataaa agtctccgga cctcttgtaa tggctgagaa catgtctgat
     8101 gccagtatcc aagacatgtg tctagtggga gatctaggag ttatcggaga aattatcgag
     8161 atgcgtggcg atgtggcgtc tattcaagta tatgaagaga cttctggaat tggtccaggc
     8221 gaaccggttc gctctactgg tgaagcttta tctgttgaat taggaccagg gatcatttca
     8281 caaatgtttg acggaatcca acggccatta gataccttta tggaaatcac acaaagtaat
     8341 ttcttagggc gcggagttca attgcccgca ttggaccatg agaaaaaatg gtggttcgaa
     8401 ccaacagtag aagccggaga aacggtgagt gctggagatg ttatcgggat cgtagaagaa
     8461 acaaaagtta taaaacataa aatcatggtg ccaaatggga taaaaggaac gatcaaaaca
     8521 atcgaaaaag gttcattcac tattgatgaa acagtatgtg tcattgaaac cgaaaatggt
     8581 gacaaaaatt tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc aatcaaagaa
     8641 aaattaaacc cggatgcacc catgatcact ggacagcgag tgattgatac attcttccca
     8701 gtgacaaaag gaggagcagc agctgtacct ggtccttttg gtgcagggaa aacagtcgtt
     8761 cagcatcaaa ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg atgtggcgag
     8821 cgtggaaatg agatgacgga tgttttaaat gaattccctg aattgattga ccctaataca
     8881 ggagaatcgt tgatggaacg tacggtattg attgctaata catcaaacat gccagtagca
     8941 gctcgagaag cttctatcta tacgggaatc acaattgcgg aatatttccg tgacatgggt
     9001 tatgacgttg ctatcatggc agactccact tcacgatggg cagaagcact tcgtgagatg
     9061 agcggacgtt tggaagaaat gcctggtgat gaaggttatc cagcttatct tggttcacgg
     9121 cttgcggaat actatgaacg atctggaaga gtcattgcat taggatcaga aggtcgcgaa
     9181 ggaagtataa ccgcaatcag tgctgtatcc ccgtctggag gggatatttc ggaaccagtc
     9241 acacaaaata cattgcgggt cgtaaaagta ttctggggtc ttgattccag tttagcccaa
     9301 aaaagacatt ttccttcgat cgattggata caaagctatt ctctttattc aacagaagta
     9361 gggaagtata tggatcatat ccttcaagct gactgggcat ccatggtcac cgagggcatg
     9421 cgtattttgc aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg gattgattcc
     9481 ctttcagata atgaccgtct gacattagaa gtggcaaaat ccattcgtga agattatttg
     9541 cagcaaaatg cttttgatga tgtcgataca ttcacttcaa gagagaaaca attcaaaatg
     9601 ttgaagatga tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt aggtgcttat
     9661 ttaagtgaga tcatggacgg taccgtatcg attcgtgaaa ggatcggacg aagcaaatat
     9721 atcccggaaa cagaattaga aaaactagat caaattattg gtgaaatcaa gcaaacgatg
     9781 caaacgatcg tttcagaagg aggcatgagc aatgattaaa gaatatcgta cgatcggtga
     9841 agtcgtcggt ccattgatgg cggttgaaaa agttgctggt gtcaaatatg aagaattaat
     9901 cgaagtccgt atgcaaaatg gcgagattcg tcgtggacaa gtattggaag tccaagaaga
     9961 taaagcaatg gtccaaatat tcgaaggaac aagtggtatc aatttacgtg actcttctgt
    10021 acgattttta ggccatcctt tagaactagg tgtatcggaa gacatgatcg gtcgagtatt
    10081 cgatggtttg ggacaaccga aagataacgg accagagatc ctgcctgaaa agcatttgga
    10141 tattaacgga gaagtaatca atccagtagc tcgggattat ccagatgagt ttatccaaac
    10201 aggtatctct gcaatcgatc atttgaatac attagttcgt gggcaaaaat taccagtatt
    10261 ttctggctct ggtcttcctc ataaagaatt agctgctcag atcgcgcgac aagcaacggt
    10321 actcgattca tcagatgatt tcgccgtagt ctttgctgct attggaatca cttttgaaga
    10381 agctgaattt ttcatggaag attttcgtca aacgggcgca atcgaccgtt ctgtcatgtt
    10441 tatgaattta gcaaacgacc ctgcaatcga acgtatcgct actcctcgta tggctttgac
    10501 tgctgcagaa tatcttgctt atgaaaaggg aatgcatgtt ttggttatca tgacagacat
    10561 gaccaactat gcagaggcat tgcgagagat ttctgcagca cggcgagaag ttccaggaag
    10621 aagaggatat cctgggtatc tttacacgaa cttagctact ttattcgaac gagcaggaag
    10681 aatccgcgga ctaaaaggat cggttactca aattccgatt ctaacaatgc cagaagacga
    10741 taaaactcat ccaatccctg acttgactgg ttatattaca gaaggacaga tcatcttgac
    10801 tagagagctt tacaagagtg ggattcagcc accaattgat gtactcccgt ctctttcgcg
    10861 gttaaaagat aagggaactg gacccggaaa gacaagagaa gaccatgcag ctacgatgaa
    10921 tcagcttttt gcagcctatg cacaaggaaa acaggcaaaa gaattagccg tagttttagg
    10981 tgaatctgct ttatctgatg tagacaagat ctatgcgaag tttgctgagc ggtttgaaaa
    11041 agaatatgtc aatcaaggat tctacacgaa ccgttcgatt actgaaacac ttgatctagg
    11101 gtgggaactg ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag acgacatgct
    11161 ggacaaatat ctgccagaag ggaagtgagt tctgatggca cgattgaatg tgaatccgac
    11221 ccgcatggaa ttgactcgat tgaaaaaaca gttgacaact gccacaagag ggcataaatt
    11281 attaaaagat aagcaagatg aactgatgcg tcaattcatc ctgttgattc gaaagaacaa
    11341 ccagctgcgt caagaaatgg aagagtctat gacagaagca atgtctgatt ttgtattggc
    11401 aaatgcttcg atcacggaac ctttcatcga agaattattt gttttgccgg cggaaaacgt
    11461 tgatttgtct atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga tgaattttga
    11521 atatgatgaa cagctgacag acacaccttt ggaatacggg tatttgaatt ccagtgccga
    11581 actggatcgg tcaatcgatc ggtttactac cctgcttcca aaacttttgg aactgacaga
    11641 aatcgaaaaa acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc ggcgagtcaa
    11701 tgcattagaa tatatgacga tccctcaatt agaagaaacg atctattata ttcgtatgaa
    11761 gctggaagaa aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa atatgggtac
    11821 cactgactaa gtggtatctt tttttaaaag aggaaaacga tgattaaaca aagcaaacaa
    11881 ataaagaaac gtttgccggc tgttcagctt ctcgcattag gattttttct cctgatttta
    11941 gttggcggcg gcctacttac cctaccattt tttagtaata gtgggcatgg gacaaatttt
    12001 atcgatgcgc ttttcactgc aacttctgct gtctgtgtaa cgggattgac aacattgaat
    12061 acagcagagc attggaacag tgctggtcaa tttttgatca tgcttttgat tgagatcggc
    12121 ggtcttggtt ttatgatggt tccgattcta tgctttgcat tgataaagaa gaaagtcagt
    12181 ctcagcacac gtattatatt aaaagaagcg ctgaatctag aagaagtatc aggtgtgatg
    12241 aagctcatgc tttacatcct aaaattggcc tttgttatcc aattgattgg tacggttgct
    12301 ttatcttttg tatttgtgcc tgaatttggc tggttgaaag gtatctggta cagtttgttc
    12361 catgcaatct ctagtttttg taatgcaggg tttgatttgt taggagacag tttagccaat
    12421 caccagcaaa atgtttattt gatcatggtg gtatctgctt tgatcatttc tggtgggtta
    12481 ggcttcatcg tatggcgtga tttactggaa tatcatcaga aaaagaagat gacgattcat
    12541 tcaaaaatcg cattaaccgt tacaggtacc cttttattag gcggattcgt tgtgttcttt
    12601 ttcacagagc gcaatggggc aacgctagtg gaagggaata tgtttcaacg attggcaaac
    12661 acctttttta tgagtgtgac acctagaacg gctggatatt attcagttga ttacctccat
    12721 atgagccatg caggtctgat tttaacgatg tttttgatgt acatcggcgg aacatctggg
    12781 tcgacggccg gcggattgaa aacaactacg ttaggtgtgt tgctgatcca gatgcgttcg
    12841 atgttgaaag gacgtacgag agcggaagca tttggccgta ccatccgaca aggcgcagtg
    12901 ttgcgggcac ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc catcatgatt
    12961 ttatcggtga ctgaaacgat acctaaaact tctggcatcg aatatattgc ttttgaggtc
    13021 ttctctgcgt ttggtacagt aggtcttacg atgggattga cgccagactt gactgcaata
    13081 gggaaattga tcattatagc gcttatgtac attggacgag ttggtgtatt gactgtggtc
    13141 ttctctctga tgacaaaagc acaaagaaca caatcaaaat acaaatatcc agaagaaagt
    13201 gtcatgattg gataatttca tcttctgaca gaaacctagt gatttagtaa aataagaaaa
    13261 taaaataatt tttcttgctt cttggatact ttaagtgcaa aatgttcgtt tattttttga
    13321 ggcactaccc gttgttgaac ctcgggtagt gcctcttttt tattgaaagg aatcaataag
    13381 atttgattcc tttatttatc aataggttat aattcatatt tttttatttt ttcttttacg
    13441 atacttagtc cagaagctaa aatttcaagg ttattagagg atagggaaat tcgaacgaat
    13501 gcatctggtt gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat accgttagca
    13561 agaaagtcat tctcaattat cttagggtca atctcaggat caactggaag aattcgatag
    13621 taagggacac gattcgttgg tttttctact ttgaaaagag aatcaaaaag ctgattggct
    13681 ctttttgtaa gacttaattt agtttctaag atcctatttg cttcatttga atggaggatt
    13741 tgagtgatga cttccacatc taatccagaa gttttcacat ttatattaaa aattgcttgt
    13801 tctatcttgc tttggacatg tactggaact acaagataag caactcgcaa tccagaacaa
    13861 ataaattttg tcattccagc taagtagatt gtttggttag gtaataaatt ttggaaagga
    13921 ggaatgatcg tatgttgata gtaagttgtt aaaaatgaat ggatatcatc ttcaataacc
    13981 catagacctt ctttttcaat cacttcggct aattgctctc gtcttttgtg agagatttga
    14041 aaccctatag gattattaca agaaggcata agaaagacac catgaatctt cttttttcta
    14101 cattcctgaa tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat tggaacgatc
    14161 tctaaatgat ataactgagc caattcaatg aaattagcgt atgtatatcg gtctgttgct
    14221 attctatcac ctggagagaa aagtgcagaa agtgtgatag ccaatccatt ttgaacaccg
    14281 gaaacaatag atagagattc taaggacgga atgactcctt gagtttttaa ccaatttgct
    14341 gcagtagtca attgaaaatt tgtgcctttg ggatcttggt aattaagcaa accaacaaga
    14401 tttgggttat tactagttga ttgaataaac gggacaacga atttattaca ttcttcaaaa
    14461 gaactaacca gaccaaaatc aatgatatgt tcggttactt cattggtaga gatggtgatg
    14521 gactcaaaga cattgggaga aataaatgtg ccacttccaa tattcgtata cagcaaacct
    14581 ttctcaattc catatttata agccttgcca acggtagtaa aatttaaatc taaataatca
    14641 gctagctctc tttgggaggg taactgagtg tttttctgta atttattctt taaaatatct
    14701 tgttctaatt gttgaatcaa cgatcgataa taaggacgct tcaatttttg tttgtctggt
    14761 cgccaagtta acgggtaatt gtcataagaa ttaataggca ttttctccac tccttaaaat
    14821 tgtataccat acaattttac cattgattgt attgatttta gaagttaatc ttttgtgagg
    14881 agggaattgt ctatgaaaat tattaatttt gaggaagtag tagcttcttt tagttttgaa
    14941 gaagcgattc aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa aatcagccaa
    15001 aatcctcgat tggtggaaat tttacctgac ggaaaaggca aaaatatgtt tgcgatgatg
    15061 cctgcttatt tgggggagaa cagatatttt ggcacaaaag tcattaccgc ttttccggat
    15121 aatcatcagt atcatttacc ttctcattta ggagagattt tcttgtttga tgctcaaaac
    15181 ggactaccca aagcattgat aaacgcaaat gcagtcacct ggattcgaac agctgctgta
    15241 tctgcgctgg caacagatta cttagcaaat ccgcatgcag aaacacttgc tttaattgga
    15301 gctggtcaac aagcattgtc gcatttgact gcgatttcta ccattcgtcc catcactaca
    15361 gtgtttgttt acgatatttt agaagaaaga cgaaatactt ttatcgctaa agctaaaaaa
    15421 cagtatccta aaataacgtt tataaatgca atatcagtag aagatgcagt acgtccagcg
    15481 gaaatcattt gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga gtgggtaagg
    15541 gaagacgcac atatcaatgc tgtgggcaca tttactcctg atacaagaga gataagtaca
    15601 tcgttgatgg aaacttctca aatctatgta gatgattact cagctgcctt aacagaaagc
    15661 ggggacctgc ttatagccat ggcagaagga gatttagagg aatcttgtat tgttggtaca
    15721 ttaggcgaat tagtttctga gaaaaaaaca ttttataaag gagaatcgag gccgacgatt
    15781 ttcgacgcag ttggtctagc tattgaagat ctttgttgtg cagaatatat ttataacaaa
    15841 ttagagaaag gggctttaaa atgaacttag attattacgt agttgatgca tttgcagatg
    15901 aggtgttcaa aggaaatcca gcagcggttt atgtgctaga agaatggctg ccagaaggta
    15961 cgatgcaaaa aattgccatt gaaaacaatt tatctgaaac agcatttaca gtgaagaaaa
    16021 accaagaatt tgagcttaga tggtttacgc cagatcgaga aatcgatctt tgtggtcacg
    16081 cgacattagc aacagcattt gttttattta actattacaa aatacctgat gaaacgatta
    16141 aattttctac tcaaagcggt aatctatttg tgacaagaat taaagattat tattacatgg
    16201 attttcctag tattatgccg aaaaaagtgc cgatattgtc tgaatatgaa gaagcgattg
    16261 gggctaagat taaagaagcc tacttagcac gtgatttatt tttcgtatta gaagatgaaa
    16321 aaacggtggc taaattgcag ccagatttta cagctatcaa aaattttgaa ttaggaatag
    16381 gagtcatcgt gacagcagaa agtgtgcaaa aagactttgt ttctagaacc ttctttccaa
    16441 aattaacaat caatgaagat ccagtatgtg gatcagcaca ttctaatctt attccttatt
    16501 gggcagaaag aatgaataaa acaaaattgt cagcttatca aatgtcatca aggggcgggg
    16561 atctagaatg tgaattaaaa gaagatcgtg tgataatagg aggaaaagca gttttgtttt
    16621 ctaaaggtac ctcgtttatt tgatttcttt caaatgaatt aaaaataaat tggtggagag
    16681 tggcaacctt atagctacaa tggttgttaa aaaaagacgc aaactttttc aatttaatga
    16741 aaagtttgcg tctcatttta attattggtg tttgtttttt attcaatcaa gagtaaaatg
    16801 ccccatacta actatcctcg gatgacttcg attttataac catcagggtc aatgacaaag
    16861 tagtaagaag gcgcagtgcc aggtaatcct tttaagtcag taatctcaag accggcagct
    16921 ttatgttgct cgtgtaattt ttctagatca tcagctgcta tagcgatatg accatagccg
    16981 tctcctagat catacgcacc atgatcatag ttgtaagtca actctaattc atagtcatcc
    17041 ccaggaagtg tcaaataaac aagtgtaaat ttgttttctg gaaaatcacg gcggcggctt
    17101 tcttcaaaac caaatgcctt ttgataaaat tcgattgatg cttcaagatc tttcactcga
    17161 acacatgtgt gagccatttt catagattca tctctccttt tccttttact caatcataac
    17221 aaaaagaagt aaggaaatag ttgatttgtg cttgttcatg agaaatttct catgtaaaat
    17281 ggatagaaaa taaaactgtt aaggtggaaa ctattcatgg ataaaccaat ttttggtgaa
    17341 aaaaagcaat ttgctgatta tcaaacccgt tatgcagcat atattgtcat agaaagagat
    17401 cagcagattg cagtaatcga ggctccaaat ggtgcttttt ttcttcctgg cggggaaatt
    17461 gaaggagaag agacgaaaga agaagcgatt gtccgtgagc tgatagaaga aatgggaatt
    17521 gtggcagaaa tttcttctta tctgggagaa gctgatgagt atttttactc gaattatcgt
    17581 gcaacttatt attataatcc aggatatttc tttgttgctg attcttggaa gcgtatagga
    17641 gaaccaactg aaaagaccaa taaaatttgg tgggtgagcc ctcaagaagc gcttgtgaaa
    17701 ttgaaacgtg gtagccaccg atgggcagtc gagagatgga tcgaaaaaaa taaaaactga
    17761 aagaaagcga gtagatcagc tttctctcag tttttttatt tattacagta catatttttt
    17821 gattgcttca gctacaccgt gctcgtcgtt tgatgaagtg atgacatttg ctgcttcttt
    17881 gacaagagaa acggcatttc ccattgctac tcctagacca gcatattcaa tcatggataa
    17941 gtcgttctcg ttatctccga tagccatgat ttcatcttga tcaataccaa ggtgctgtgc
    18001 gagattcgca actgccgcac ctttacttgc ttctttattt agtacttcga agtaataggg
    18061 cgcacttttg accgttgtgt atttatcacg gaaagtttgc ggtaaacgcg caattgctgc
    18121 atctaaaatt tctggttcgt cgatcatcat cattttgacg atgttcatgt ctggtgtcat
    18181 ttcttcaggt gttcgatatt ttagcggcat cttgactaaa cttgcttcgt ggatcgtata
    18241 agcgctgata tctcgattag gcgtgtagat cgctttgtca tcgatcgaat ggagatgact
    18301 gccgacacgg cgtgccatca cttcgatctc taggaaatct tcatgtgtca gtccgtgacg
    18361 ggcgatgatt ttacctgatt tcgtttgttg tacgagtgcc ccgttataag taataacgta
    18421 atcattatct tgaaaaaggt tcaactcgtt taactgttcc tggacacctg gtaagggacg
    18481 gccagtgcat aaaacgatcg aaacaccttt tgcaacagca gcggcaatcg cttcttttac
    18541 ttcttttgtt acttctcttt tctcatttaa taaagtacca tcgatgtcga tagcgactag
    18601 tttgatagac ataatttcct ccaatatatt ttaatttatt tcaatcaatg cgccattacg
    18661 gatatggctg gcaaattctt gataagtttc gtcaaataaa tcataatgat cacgcaagga
    18721 cggatcaacc atttcttttg gaaagaaaaa tcgctcatct ccttgttctt gtcccgctaa
    18781 tgcagcaaca agctgactaa cctgtgataa ttctatcaaa gttccgtcat ttcgcaaaat
    18841 ctcgatttgt gtgcgatgtc tgccttgttc tgggcgataa aaatcataag gtagatcgta
    18901 gctagaattc acagcagtgt aataaacagg attatatccg accttttcta ctaacaaagt
    18961 gagttcttgg actaattttg aatcccggtt gccagaaaaa gtagcagatt tcagtggttt
    19021 acgattcaaa aaacgtttag ctaaatcatt taaaatagga tcagcagatt cagaccattg
    19081 agtaaagtaa gtgcccaaga ccccatcatc aagtttgagg tattcttcta aagtaaagct
    19141 tccgtcaaga aatggcagaa gtaattgaga atggagttca aaactgctgg catcttcttg
    19201 atacaactcg tgtgcgcgat gaagcagatg ctccaaaatt acttccatac cacgggaaac
    19261 aggatggaag tacacttgca catacatttg atatcggcta acgatataat cttccactgc
    19321 atgcatgcca ctcatggaaa aagcgatccc gccttcataa ggacgaatca cacgtaatat
    19381 tcgggtaaga tcaaatgttc catattctgt tccagtgaaa taagcatccc gaagcaaata
    19441 gtccattcga tctgcatcga tctgactaga aatcatttgg acaacttgtg ggttggggta
    19501 agttttttgg atcacactag ctaccttttc aggaaaacct tcttctactc ggttaagaat
    19561 ctgatacact tctgtttctg gagaagtgat gatctctaca gtgatggctt catgattcgt
    19621 atggaagata tgttcaaatg tatgagagta aggaccatga ccgacatcat gcaacagtgc
    19681 cgcacataaa gtcaccagcc gttcctgatc atcccagcct ccgttgccta ttttttcttt
    19741 tgaaaaattt cgagaaaaga tatcgcagat tcgtcttgaa atctcataaa cgcctaatga
    19801 atgagtaaaa cggctatgtt ctgctccgtg aaaagtgaaa gaagaagtgc ctaattgttt
    19861 aatgcgtcgt agacgttgga cttctttcga attgatcaaa tctaagatta cttgatgctg
    19921 gacatggata tagttatgga ctggatcacg aaaaactttt tccataggca gcatttgata
    19981 tttataaggg gttgtcataa tgtagctcct ttatgttatt acgttgttcc attctctgga
    20041 tttggttgtt ccattgctcc agctggattt ctttttttag ccagtcggta gaatcctgta
    20101 aatcacttgc agaatacagc ttttcaaaag cttttgcaaa tgcttctttg acttctttta
    20161 caaaaagggg ttggtcaagc agttcttcaa gtgttgccat tgtagcagga tccactgcag
    20221 gatagccatt tgtaccaaag tcttctttta aagcagattg ataaaatcgc ttcatgactt
    20281 gcccgcgttt ttcctgatta ccgttgacac ttaagtacat cataacagaa atcccatttt
    20341 tgatccgacg ctgagcgata ccggcaaatt ttttgccatg gatgcttaaa tcataagttc
    20401 caggacaata agaatgggtg atttcgaatg cttgtatcgg tgcctttgga aaagcgtatt
    20461 tagtcaatag gagcatttgc tcgtaagcgt catctatggt tatttttttt gtgtcacttt
    20521 gggggaaaat caaagaaata ttcaaaattc cttcatctga tacgatccct aatccacctg
    20581 aatttctgat cactggagcg taacctgcat ctttcagtgt ttggatcccg tcacttagaa
    20641 aaggagttcg aacatccttc atacctaaga tgactgtgca gtcatattgc cagaaatgta
    20701 aaaagatttg ctggtgttct ttagaatatt ccgttaatat atctgttaat gcaaaagggg
    20761 aaaagtcgtt tttttgataa atgtgctgat ctagtcgaat cgcagggttc attcaaccgg
    20821 ctcctttgta taaattcata ttttctacaa tattatactc taaaatcaaa gagttgtttt
    20881 ggaaaaaagg ttttctgtaa aaatggacaa gttggttgac aaaacaaaag aaactaggaa
    20941 caatgtagct atgagaagta aaggagaatg aataatggaa ttatcacaag gaacaccgat
    21001 ttcaatcaaa ttgcgtacaa aagtaaaaca gcaaggggaa gtgcaagatt tttattttga
    21061 tttgaaaggg caaatggtga caattggcga tacgctgtac atacgttata aagagattca
    21121 ggaggagacg ggcgatgaga ttcctgtaac gatcaaatta gttccagatg gtcatgtaca
    21181 gctgatccgt gcaggagaaa tgcggatgcg tttgaaattt ggctataaag aacggttaga
    21241 aacatcctat cgaacaccat acggtatgat ccagattgct acttttacga aagaactgca
    21301 tgttagtttg aaagaccgtc ccacaggagg gaaggttcga atcgattatg atctgttcat
    21361 gggaccggaa aaaatcggcg aatattattt gaccttggat tttacagcgt aaatataaaa
    21421 gaaaagtttg attttttgtg tctgattttg tatgataagg agtagactgt gaaaggacgt
    21481 gtcctgattg gaaattagtg tatttgaagg tgcaaacaaa agcgaattat caatgatcga
    21541 agtagcacac gcgatcttag aacaacgtgg cgatgtcatg gatttttctg atttagctaa
    21601 ccagatccaa aactatcttg ggaaatcaga cagtgaaatt cgtgattcac tcgcacaatt
    21661 ttatactgac ttgaatattg acggcagctt tatctcatta ggggataatc gttggggctt
    21721 gcgttcatgg tatgctatcg attcgatcga tgaagaagta aaccatggaa tggacgaaga
    21781 ggatgaagat actccacgtc gccgtaaacg caagaaagtc aacgccttta tcaatgatga
    21841 tgaagacgcg atcgattaca atgacgatga cccagaagat acagatctga cagaagaaga
    21901 tgacgatgat ctgtttgatg acgatgatga tgaagatgaa gaaatcgctg cttataattc
    21961 tgacttgcaa gaaatcggag cagatgaccc gacagatgat gaagaagact tgccaggaaa
    22021 tatcgaagaa gatttaagta tcatagacga agacgatgat gatgaagatt atgaagagga
    22081 agaattttct gactctgaaa aagaataaac attattttct tacgagaggt tgtgacagag
    22141 acgatcagcc tatcagaata aggaggtgtg acacgatttt tgtcacacct tcttttttgt
    22201 atataaaaat cccaagtaaa tacataaaaa ataaaaaaat tgaaactttt agcgcacctt
    22261 gtacgtcata ttaagtgagg cagaaaggag tggagaatga tttttactgg atataagaaa
    22321 aaacggcaga tcgaaaaata ccgtcacctg ctgaatggac tcatcgaaac tgattatcaa
    22381 aaaatgttcc gtatcacgat gaattacgta catgataaag aagaggcctt agatgtgatg
    22441 caagatagtt ttcataaagc cctgaactat tttgaaaaag aaaaagagat cgagcatttt
    22501 agtgcatggt tttatcggat attgatccgc acggcattag atagttggcg cagacaaaaa
    22561 aggacgcctg tggacttatt tgaaattcaa gatatccccc aacagttacc tttagaaact
    22621 tccgttgcag agctccatat gattttagat aaaatagaca gtccggaaaa agagattctg
    22681 atcctctact tttttgaagg atttcgtttg aaagaaattg cgactatctt ggatatgaat
    22741 gaaaacacag ttaaaacgaa aatgtaccgc tcattgaatg ctttacgtca aatcctagaa
    22801 tgaaggagag aagagtgcca tgtattccca aaaagaaaat ttaaaaaaac taaagaaaac
    22861 ttataaagaa caagcaattc ccaaagggct taaagaggag atccgccagc gttttattat
    22921 cgaagagcaa tcatttataa aagcaaagcg gagaaagaga cgtctccaag ggttcggagt
    22981 atctttggct atcgtaatga ttacaacaag tagccttctt tttaatagtc aagttcgaag
    23041 ctttgcagaa gatttaccca tcttaggatc agtcattgaa ttgatcttag gcgaacgctt
    23101 tactgatcga tctgaaaaaa tcgatattca ggtaccgaag atcaatacac aaaatgagaa
    23161 agaaaataaa acaattcatg gattaaatca aaaatacttt cgcgaaggac aagcagattt
    23221 tgaaaaagct gaaaaagaat atggaaattt tgaaacggat cattaccaag ttttgggaga
    23281 ttatcagaaa atcgtagacg ataaccggtt tctcgtgatt gaaaggcaga tcacccaaac
    23341 agcagcagat agccatgttg aaaaaagata cgacacaatc gacaagaaaa acagtgtcca
    23401 gctctcactt ccacttttat tcaaagatga cacctatcta tctgttttga caaaagaagt
    23461 caaacggcag atggccgaac aagtcaagga agatccatct aagtactatt ggactgagca
    23521 agatattcaa gaagggacga tagaaaaacc aacacttgtt acgcctacac gaagttttta
    23581 tttgaacaaa gagcatcaat tagttttgac tttttctcaa tacgaaattg ctcctggtta
    23641 tatgggtaca ccagaatttg tcattcctaa gtccgtgaca aaaacgattt tggcatcgga
    23701 agattatttg gatcattaga cgagaagtag ttgcgactag cagtttcaat tttcaagtaa
    23761 gtacaagtaa ataataagaa gagaaaaaga gaaagaagag atatagtgaa aagaaattat
    23821 ttagttatca gtattattgc tttatccgtg cttttgtttt tcgaaattac ttcggtggcc
    23881 tccttgatca ccgaagtaaa aggacaaaca cactcttcgg agcattcgtc acaatctttc
    23941 aaacaatcag aacaatcaac cattaaaagc caatcaactg aaacagctaa cgtcaaagaa
    24001 gccattcaag aatcagcgga aaaggtacaa agtcagtcat cacaatcaac acaagacagt
    24061 acaaaaacga gccgagatcc ggctgtcaaa agcgtcgcaa tcagctttga tgatggacca
    24121 ggagcgacca caacaccgca gcttctgcga atcttaaaag aaaaaaacgt gcatgccact
    24181 tttttcgtct taggagaaaa tacggcgcag catcctgaaa tcgtgaagca gacagctgaa
    24241 gcagggcacg agattggcaa tcacacatac gatcatcaga atctggccac tctttctgcc
    24301 cagtcgatga cggaggaagt gacgaaagca gacactgaga tcaaaaaagt cactggcaaa
    24361 acccccactt ttgttcgtcc gccctatggt tctgtcacga atgtaggagc aacgatcatc
    24421 cagcgaccga tcatcgaatg gtctgttgat tcagaagatt ggaagacaag aaatcctgat
    24481 ttgattttgc agaagatcca agcaacagta tacgatgggg caattatttt attccatgat
    24541 atctatcctg aaacgattcg tgccgttcct caagtgatcg attatctgaa agagcaaggg
    24601 tatcgcatca cgacagttgg ggatttatta gggcatccaa cggctgtcga gaactattat
    24661 ggcagaaacg atcatcggcc agttcagtaa gtgagacaag tatctttaat aaataacaca
    24721 ttttaataag tagttgaaca cataactacc ttacttgcaa aggcttaaca atatctatac
    24781 aggtacaaca aaaaactgtc caccaatttt ctttggcgga cagttttcac ttaaaaataa
    24841 agaagcaact attcgtaaat tatgctaaaa tagcagtaga acgcgtcctg agggaatcga
    24901 acccccgtct caagaaccgg aatcttacgt gatatccact acactaagga cgcaagcact
    24961 gggataattg taactaaaca gataaaaaat tacaagggga ttggacaaaa aaatgaaatt
    25021 tgagcaaaat ttttcacaaa aacagcagca gacacagaaa ctagccatga cgcaacagtt
    25081 gcaacagtcc atccaagttt tacaatataa cagtgaggag ctgttggcat tcgttgagaa
    25141 tcaagcaatg gaaaatccat tggtggaagt tgttgagccg gaatggcagc cagattacat
    25201 aaaagcttct tcatcttctt atgaaggaga agagacaaat tacctaaatc agattcctga
    25261 tacaaaaggg tctttatttg attcattgat cgaacaagtc catttgaatt accgagatac
    25321 ttttttgagg aaaatcgttc tttatcttgt agaatacatc gatttgaacg gatttttgac
    25381 gattagttta gaagaagccg gaaaagagat tggagctacg cctatccaaa tgctcgatgc
    25441 gttgactctg attcagcaat tagaaccagc tggagtaggt gcacgtagtt tgcaagaatg
    25501 tctcatgctt caaacagaac gagatgatta tgcgccggaa ttagcttata tcgtgttgga
    25561 agaatgtttt gaggaactgg tggagcgaaa atggaaagaa atcgcacaac gtttttctgt
    25621 tgatctacat gctgttcagc agatattcga ctatttacaa acgttgagtc caagccctgg
    25681 ccggattttc gaccgtagca gcgaattgta cattattcct gatgtccggg tattagtgga
    25741 cgacgataaa aatgtacaag ttatctcaaa ccgaaaaaat cagccaaata tacgctttca
    25801 agagagttac ttcaaacaaa tgtcacaaca agcagataaa gaaactgaaa attatttaaa
    25861 agaacggaaa caggaattcg aatggttgaa gaaaacgatc ctacaacgtg gggatacaat
    25921 tttgcgggtt gctcaagtca ttgtttcccg gcaaaaagct ttctttatcg acaaggaacg
    25981 tccaatcaag cctcttacgt taaaggaagt tgcaactgag atagatgtgc atgaatccac
    26041 ggtcagtcgt gcagtcaacg ggaaatattt ggaaacagac ttcggtgtat ttgaattaaa
    26101 aaaatttttc actacacgaa ttcctacgaa tagtacagaa cagacagaag acctttcagc
    26161 agatacagcg aagaaaaagc tgcaggaatt agtcgatcaa gaagacaaaa acaagccgtt
    26221 gtctgaccag aaattggttg aattgctgaa aaaagatgag atagcgatct caagacgaac
    26281 agtagcaaaa tatcgtgatt tattaggaat cccgagttca tcaaaaagaa aacgttatga
    26341 taactaatca gtcaaaagac tagaaaaagt atttcacttt ttctagtctt tttagttgaa
    26401 aaacgagagc aaaactgtta gacttacagt cgtgaggttg agatttccct ttattgatgt
    26461 gaaattcgaa tagttaattt ctttttttat cgtatatggg tcgttttaag tctacattgg
    26521 acgtaaagtg tccaactaag gagatcgcta tgctggatga aataaaaatg attgaatctg
    26581 ttgcaccaga tattatcgaa gtgttgcaag aacgctataa aattttacga aatatctatt
    26641 ggatgcagcc gattgggcgt cgaagtttat cggaaagtat ggggatcaca gaacgagtct
    26701 tacgaacaga aacagacctt ttgaaaaatc tgaatttgat cgacacctca aaaagcggta
    26761 tgacgctgac aactaagggc gaagaagttt atcaaagcct tgaaaacttc atggatcagc
    26821 ttttaggtac gcatcaaacc gaacaacagt tggcagaata ttttggtaac caacgctgta
    26881 tcgttgtttc aggaaacagc gaagagcaga ccaaagtggc tgatgcattc ggcgaagcgc
    26941 tgagcgaagc cttagaccga ctacttccag aaggcgagaa tatcatcgct gtgatgggag
    27001 gaactactat ggctgtggta gccgaacaat tgtctaatct ggaaaataag aaacggcata
    27061 acctgtttgt ccctgcaaga ggcgggattg gtgaagcgat aactgtacag gcaaattctg
    27121 tcagtgcaag aatggctgct aaagcaaacg gtaaccaccg cgcactttat gttccggaac
    27181 aattaagtct ggcaacttac aattctttac tcaatgagcc atctatccaa gaagtcttaa
    27241 acttgattag tgaagcgaat tgtgttattc atagtattgg gcgtgcatta cacatggcag
    27301 cacgtcgtaa gatgactgaa aaagaattag tcatgctgaa acaagcaaat gctgttgcgg
    27361 aatcttttgg ctactttttc aatgaaaaag gtgaagtcgt ttataaggtc cctagaatcg
    27421 gcatagaatt gcaagatttg gaaaaagtcc cgatcatcat ggcaatcgcg ggcggaaaat
    27481 caaaagctaa agcaattcga gcttatatga aaaatgcacc aaaacaaacg tggctcatca
    27541 ctgatgaagc tgctgcaaat gagattttaa aaggggtaac cctttaaaat aaatattttt
    27601 ttgattttca taaggaggaa atcttaaatg acagttaaag taggtattaa tggttttgga
    27661 cgtattggac gtctagcatt ccgtcgtatc caagatgtag atggaatcga agtagtagca
    27721 atcaacgact tgacagatgc taaaatgttg gcacacttgt taaaatacga cacaactcaa
    27781 ggacgtttca acggaacagt tgaagttcat gaaggttcat tcaacgtaaa cggaaaagaa
    27841 gttaaagtat tagctaaccg caacccagaa gaattaccat ggggcgaact aggcgtagat
    27901 atcgttctag aatgtactgg tttcttcact tctaaatcag cagctgaaaa acatttaaca
    27961 gctggtgcta aacgcgttgt tatctctgct cctggcggaa acgatgttcc aacaatcgtt
    28021 tataacacaa accacgaaac attaactggt aaagaaacag ttatctcagg tgcttcatgt
    28081 actactaact gtttagctcc tatggctaaa acattgaacg acaaatttgg tgttgttgaa
    28141 ggattaatga caactatcca cgcttacaca ggtgaccaaa tgactctaga cggacctcat
    28201 cctaaaggtg acttccgccg tgcacgcgct gctgctgcaa acatcgttcc taactcaaca
    28261 ggtgctgcta aagctatcgg tttagtaatt cccgaattga acggtaaatt agacggagct
    28321 gctcaacgtg ttcctgtacc aacaggttca ttaacagaat tagtaacagt tcttgaaaaa
    28381 gaagtaactg ttgacgaaat caatgcagca atgaaagaag cttcaaacga atcttacgga
    28441 tacaacacag acgaaatcgt ttcttcagat atcgttggta tgacttacgg ttcattattt
    28501 gatgctacac aaactaaagt aatgacagtt ggcgacaaac aattagttaa aactgttgct
    28561 tggtacgaca acgaaatgtc atacactgca caattagtac gtactttaga atacttcgct
    28621 aacttataag atagacaaac gtacaagtgt aaattataga catgtacatt taaaagcggg
    28681 gaagcaatcg cgcttcttcc gctttttttg ataataagtt tattcaggag gaaataaaat
    28741 ggctaagaaa acagtgaaag atatcgattt aaaagacaaa aaagttcttg tccgtgttga
    28801 cttcaacgtt cctttgaaag acggcgtgat cactgacgac actcgtatca aagcagcatt
    28861 gccaacaatc aactacgttc tagaacaagg cggaaaagca atccttttct ctcaccttgg
    28921 acgtgtaaaa acagaagaag ataaagaagg aaaatcttta gctccagttg caaaacgtct
    28981 aggcgaatta ttaggtaaag aagtaacatt cgtacctgaa actcgcggag aacaattaga
    29041 agaagcaatc cgcaacatga atgacggcga cgttgtcgta ttcgaaaata cacgatttga
    29101 agatattgac ggtaaaaaag aaagcaaaaa tgatacagaa ctaggtaaat actgggcttc
    29161 attaggcgat gtatttgtta acgatgcttt cggtacagct caccgtgcac atgcttctaa
    29221 cgtaggtatc gcttcaactg gtatcccaac agttgctgga ttcttgatgg aaaaagaaat
    29281 caagttcatc ggtgaagcag tagaagaacc aaaacgacca atgattgcaa tccttggtgg
    29341 cgcaaaagtt tctgataaaa tcggcgtaat cgaaaacttg attcctaaag cagataaaat
    29401 cttgatcggt ggcggaatga cttacacatt ctacgaagca aaaggaatca agatcggtaa
    29461 ctctctagta gaagctgata aagtggaact agcaaaagaa ttaatcgaaa aagcaggcga
    29521 caaacttgtc ttgccaatcg acaatgtctg tgcaccagaa ttttcaaatg atgttgaaac
    29581 acaagtaatc gaaggagaca ttcctgatgg tttgatggct ttagacatcg gcccagcatc
    29641 agtaaaatta tttgctgata cattacaagg tgcaaaaaca gttgtttgga acggacctat
    29701 gggtgtattt gaaatgagta actttgctaa aggtacaatc ggtgtttgtg aagcaatcgc
    29761 taacttagaa ggcgcaacaa caatcatcgg tggcggagac tcagccgcag cagcagaaca
    29821 attaggattt gctgacaaat tcacacacat ctcaacaggt ggcggcgcaa gcttggaatt
    29881 gcttgaaggt aaagaattac caggactagc agcaatcaac gataaataaa cgaccaagtt
    29941 tgctcaattc tgacgagtaa tcgaacaaaa ggtgaaggga gttttcatga ctccctagcc
    30001 atttttcaat aaattaagga gtgtgttcct caatgcgtaa acctattatc gcaggtaact
    30061 ggaaaatgaa caaaactgca tcagaagcaa aagaatttgc tgaagcagta aaaaacaaaa
    30121 tcccttcaaa tgacaaagtt gattcagtca tcggatctcc tgcattattc ttacaagaat
    30181 taatgacatc agctgaaggt acagaactaa aaatctcagc acaaaactgc tactgggaaa
    30241 actcaggtgc gttcactggt gaaacttcac cagcagctct tgctgatcta ggtgttcatt
    30301 atgtgatcat cggacattca gaacgtcgtg aatatttcca tgaaacagac gaagacatca
    30361 ataaaaaagc gaaagctatc tttgcaaatg gtatgacacc aatcctatgc tgcggcgaat
    30421 ctttagaaac ctatgaagca ggcaaaactg ctgaatggat cgaaggacaa attacagctg
    30481 gtttagctga tctttcagct gaacaggtgt caaacatggt tattgcttat gaaccaatct
    30541 gggcaatcgg tactggtaaa tcagctgacg cacaaatcgc tgacgaaatt tgtggagttg
    30601 tacgtcaaac agttgaaaaa ctttatggaa aagaagtatc agaagcagtt cgtatccaat
    30661 acggcggttc tgtaaaacca gaaaatattg ctgaatacat ggcaaaagaa aatgttgacg
    30721 gtgctttagt cggtggcgca agcttgcaag cagattcatt cttgtcattg ttagacgctg
    30781 taaaataatt gttgaaaatt tgcgaaaaat gcaagaaatg tttgcactaa tgagcaaaat
    30841 taactaaaat cgtatatgag ggaaatccct taggaaaaaa caaactcaaa ggagagacaa
    30901 aacatgtcaa ttattactga tgtttacgct cgcgagatct tagactcacg tggtaaccca
    30961 acaatcgaag tagaagtata cacagaaagc ggagcttttg gccgcggaat ggttccttca
    31021 ggtgcttcaa ctggtgaata cgaagcagtt gaattacgtg acggagacaa agctcgttat
    31081 ggcggtaaag gtgtaactaa agctgtcgac aacgtaaaca acatcattgc tgaagcaatc
    31141 attggctacg atgttcgtga ccaaatggct atcgacaaag ctatgatcgc tttagacgga
    31201 actcctaaca aaggtaaatt aggtgcgaac gctattttag gtgtttctat cgctgttgca
    31261 cgtgcagctg ctgactacct agaagtacct ttataccact acctaggcgg attcaacaca
    31321 aaagtattgc caacaccaat gatgaacatc atcaacggcg gatcacatgc tgataactca
    31381 atcgacttcc aagaattcat gatcatgcct gttggcgctc caacattcaa agaagctcta
    31441 cgtatgggtg ctgaagtatt ccacgcatta gcttcaatct tgaaagctcg cggattagca
    31501 acttctgttg gtgacgaagg tggatttgct ccaaaccttg gttcaaacga agaaggtttt
    31561 gaagtaatca tcgaagcaat cgaaaaagct ggctatgtac ctggtaaaga cgttgttctt
    31621 gctatggatg ctgcttcttc agaattctac gacaaagaaa aaggtgttta cgtactagct
    31681 gactcaggcg aaggcgaaaa aacaactgac gaaatgatca aattctacga agaattagtt
    31741 tctaaatatc caatcatctc aatcgaagat ggattagacg aaaacgactg ggacggattc
    31801 aagaaattaa ctgacgtatt aggcgacaaa gttcaattag ttggtgacga cttgttcgta
    31861 acaaacactc aaaaattatc tgaaggtatc gaaaaaggaa ttgctaactc aatccttatc
    31921 aaagtgaacc aaatcggtac attaacagaa actttcgaag ctatcgaaat ggctaaagaa
    31981 gctggctaca cagcagttgt atctcaccgt tctggtgaaa cagaagattc aacaatctct
    32041 gatatcgctg ttgcaacaaa cgctggccaa atcaaaactg gttctctatc acgtactgac
    32101 cgtattgcta aatacaacca attactacgt atcgaagacc aacttggtga agttgcagaa
    32161 tacaaaggtt tgaaatcttt ctacaactta aaaaacaaat aattcttttc taatgaagat
    32221 acatgatcga ctattgaaag atagtcgagt atgatcactt agttaagtaa tcgaactctc
    32281 tacttgctga aatatcagca ggtagagagt ttttttgcaa ctagcttctg tatatgatgt
    32341 ttacaaagaa tggtacaaga aaagatcatc cttgaaaaag gaaagctaac aataactagc
    32401 atcatataag agcttttaag gatagaacag tagaatagga atctatgggc aagtcctctc
    32461 gtataaa
//
LOCUS       DADXTH010000025        31967 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.025, whole genome shotgun sequence.
ACCESSION   DADXTH010000025 DADXTH010000000
VERSION     DADXTH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.025"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            126..3353
                     /gene="fss3"
                     /locus_tag="IWI97_001161"
     CDS             126..3353
                     /gene="fss3"
                     /locus_tag="IWI97_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBC2462679.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3465..3779
                     /locus_tag="IWI97_001162"
     CDS             3465..3779
                     /locus_tag="IWI97_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBC2462680.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3792..4166
                     /locus_tag="IWI97_001163"
     CDS             3792..4166
                     /locus_tag="IWI97_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBC2462681.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4167..4520
                     /locus_tag="IWI97_001164"
     CDS             4167..4520
                     /locus_tag="IWI97_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBC2462682.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4589..5605
                     /locus_tag="IWI97_001165"
     CDS             4589..5605
                     /locus_tag="IWI97_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBC2462683.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6199..8073
                     /gene="ltrA"
                     /locus_tag="IWI97_001166"
     CDS             6199..8073
                     /gene="ltrA"
                     /locus_tag="IWI97_001166"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBC2462684.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8199..8555
                     /locus_tag="IWI97_001167"
     CDS             8199..8555
                     /locus_tag="IWI97_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBC2462685.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8600..9742
                     /gene="dcm"
                     /locus_tag="IWI97_001168"
     CDS             8600..9742
                     /gene="dcm"
                     /locus_tag="IWI97_001168"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBC2462686.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9732..10021
                     /locus_tag="IWI97_001169"
                     /pseudo
     CDS             9732..10021
                     /locus_tag="IWI97_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10277..11461
                     /locus_tag="IWI97_001170"
     CDS             10277..11461
                     /locus_tag="IWI97_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBC2462687.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11458..11571
                     /locus_tag="IWI97_001171"
     CDS             11458..11571
                     /locus_tag="IWI97_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBC2462688.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            11600..11821
                     /locus_tag="IWI97_001172"
     CDS             11600..11821
                     /locus_tag="IWI97_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462689.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            11834..12337
                     /locus_tag="IWI97_001173"
     CDS             11834..12337
                     /locus_tag="IWI97_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBC2462690.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            12464..13501
                     /locus_tag="IWI97_001174"
     CDS             12464..13501
                     /locus_tag="IWI97_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462691.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            13516..15213
                     /locus_tag="IWI97_001175"
     CDS             13516..15213
                     /locus_tag="IWI97_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HBC2462692.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            15206..15667
                     /locus_tag="IWI97_001176"
     CDS             15206..15667
                     /locus_tag="IWI97_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HBC2462693.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            15680..16540
                     /locus_tag="IWI97_001177"
     CDS             15680..16540
                     /locus_tag="IWI97_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462694.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            16578..16967
                     /locus_tag="IWI97_001178"
     CDS             16578..16967
                     /locus_tag="IWI97_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2462695.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            16951..19401
                     /locus_tag="IWI97_001179"
     CDS             16951..19401
                     /locus_tag="IWI97_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBC2462696.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            19406..21514
                     /locus_tag="IWI97_001180"
     CDS             19406..21514
                     /locus_tag="IWI97_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBC2462697.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            21511..22515
                     /locus_tag="IWI97_001181"
     CDS             21511..22515
                     /locus_tag="IWI97_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBC2462698.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            22534..23445
                     /locus_tag="IWI97_001182"
     CDS             22534..23445
                     /locus_tag="IWI97_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2462699.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            23632..23919
                     /locus_tag="IWI97_001183"
                     /pseudo
     CDS             23632..23919
                     /locus_tag="IWI97_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(24107..24322)
                     /locus_tag="IWI97_001184"
     CDS             complement(24107..24322)
                     /locus_tag="IWI97_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462700.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(24581..24748)
                     /locus_tag="IWI97_001185"
     CDS             complement(24581..24748)
                     /locus_tag="IWI97_001185"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2462701.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(25093..26532)
                     /locus_tag="IWI97_001186"
                     /pseudo
     CDS             complement(25093..26532)
                     /locus_tag="IWI97_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(27080..27751)
                     /locus_tag="IWI97_001187"
     CDS             complement(27080..27751)
                     /locus_tag="IWI97_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBC2462702.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(28100..29551)
                     /locus_tag="IWI97_001188"
     CDS             complement(28100..29551)
                     /locus_tag="IWI97_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBC2462703.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(29663..31005)
                     /locus_tag="IWI97_001189"
                     /pseudo
     CDS             complement(29663..31005)
                     /locus_tag="IWI97_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(31139..>31967)
                     /locus_tag="IWI97_001190"
     CDS             complement(31139..>31967)
                     /locus_tag="IWI97_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2462704.1"
                     /translation="TPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDAD
                     KYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYP
                     DGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTA
                     DKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQ
                     GSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT        10436 a         5454 c         6367 g         9710 t
ORIGIN      
        1 tttaggctca ttcattctca aatccattct atttagtcaa taaatatttt aaaaagactt
       61 tataaacaaa gatgttcaag cgagcatctt atttttttgt gcctaatttg aaaggagaac
      121 atttcatgta taaaaaacaa aaaaagaaat tatgtgtatt aactattttg agtttggctg
      181 ttcaatcaat tgtaggagca attgctccta cgattgcttt tgctgatgaa attacccatc
      241 cacaaacggt aaccgtagaa ctagatttag cacatcaata tgctgttgaa ggaactttta
      301 gtgatggtcg tccaatgtca gaagtcactg ttccgcatta tgcagtgtac aacggcgtta
      361 aacaagatat tttctgtgtt gaaccaggag tcccgattta caatgagttc actcctggat
      421 atgagaagaa cccgttacca gatatgtccg ataaagccaa attagtttcc gttttatgga
      481 aaaaagtagg tacagatgca gatactcata ttgtggcaca gaagatgatc tggcaagaag
      541 ttaatgggta tacccttcac tcaataaaac gatcagatgg tagtgccgta aatatcgcag
      601 ccattgaggc aaaaatcaat caagccattg ccgattatca gaagaaacca agcttccata
      661 atagtacggc taaaacagta ttagggcaat cgactactgt gacggatacg aataatctga
      721 atttgtcaga gttcgatgag gttgtggaaa ataccgcaaa cattgattat cgtgtgaatg
      781 gcaatcagtt gataatcagc ccaaatgcca attcaaaaga aagtggtgtg ttgacgctta
      841 agaaatctgc tggtaccgga acacctgtcg cctataaaaa ggctggccaa cagactttaa
      901 tggccggggc gatcgataag ccgaacactt atacggtcaa aattgatgtg gaaatggagg
      961 gttctttgaa gattaaaaaa gttgataaag aatcgggtga tattatacca ggaacggttt
     1021 tccatttaga ctttggaaaa actttacccg caaaagacgt gaccaccgat aaagaaggca
     1081 ttgcgacctt agatgagatt cctcatggta caaaggtaac cattactgaa aaatcggtgc
     1141 cggcgcctta tacgattgat accacaccca tgactgccac aattaaagca ggtgaaacca
     1201 tttcagtgac ttcaaaaaat acgcgagaaa aaggtcaaat tattctggat aaaaccggaa
     1261 ttgaaacagg gagtgatctt tggaacgata attactcttt agcgggcaat acgtttgcca
     1321 ttcgcaagga tagcccaact ggtgaaatcg tccaagaaat gaccacggat gaaaaaggtc
     1381 atgcagaaac accaaaagag attgccaatg cgttggaatt gggaacctac tatgtgactg
     1441 aaaccaaggc tagtcatggc tttgtgaata ctttcaaacc agtaaaagtc gaattgaatt
     1501 atgtcaatca aacagtggct cttgttacca gtaacgtaaa agggcaaaac caagaaatta
     1561 ctggtgaaac cactttgaca aaagaagaca aagataccgg tcatgaaact caagggaaag
     1621 ctgagtttaa aagagctgaa tacaccttgt ttactgttaa ggatggaaaa gcggtcaaat
     1681 ggaccgagaa ctttaaacca gaattgttga agggaacgaa agcttctgat gaaaccgtaa
     1741 ctttgacttt agatgaaaag aatcaagttg ccgttaaaca cttagcgatt aatgagtatt
     1801 actggcaaga aaccaaagcg cctgaaggat ataccttgga tgaaacaaag tatcctgtat
     1861 ccatcaaaaa ggtggatgat agcgaaaaaa atgctgtgat tactcgagat gttgcggcaa
     1921 aagaacaagt gattcgcttt ggctttgatt tctttaaatt tgctggatca actactggca
     1981 ctgccgaaac tggcttcaat gatctaacct ttaaagtgtc gccattggaa ggaaccaatg
     2041 aaatcaccgg tgccgaagat gaagcgacca cagcttataa cgagcaatta gggtttgatg
     2101 gttatggcaa gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaagccc
     2161 cagaaggttt tcaaaagatt acgccattag aaatccgttc tacttttaaa gaaaataaag
     2221 aggattatac caagagtgat tatatcttta ctattaccga aaaagggcaa aagcaaccga
     2281 ttaaaacggt aacggtccct tatgggaatc taaccaatga gaatttctca gtcagtttga
     2341 accgtttgat gctttatgat ttgcctgagg aagaagatag cttaacttct cttgcgactt
     2401 ggaaagaagg agataaagaa ttgactactc ttgattctac cgagctagtc gataaattga
     2461 gctataatct acatgaaatc aaagaagact ggtatatcgt agctcaagcc atcgatgtag
     2521 atgctacaaa agctgcccaa gaaaaagata aaaaagccaa accggtggcg attgcggaaa
     2581 caaccgcaac tttagcgaat aaagagaaaa caggaacttg ggagattctg cataaattaa
     2641 ctgtcgaaca agttttagac aagaccattg tcttattcaa ctatgtgtat gaaagcaaag
     2701 aagcctatga agctggtgat gagccggtag cgaaggatgc tagcttgaac aatcaagcgc
     2761 aaactgttaa ttgtatggtg gaacgtcatg tttccatcca aaccaaagcg cacttagaag
     2821 aaggttctca gacctttact catggtgacg tggtagatat gtttgatgat gtatccatta
     2881 cccatgatgt actagatggc tcaaaagaag cttttgaaac aattctgtac gctttatttc
     2941 cagatggtac aagtaaagaa atttggaaat ctggcaagat tgattacgaa gtgaatgaca
     3001 aagaatttac caaaaccgta cttgcggaaa aagtggatac aagtaaatat ccagaaggta
     3061 caagttttac cttcaaagaa attaactatg acaaagatgg gaatatcaat ggaaaacaca
     3121 atgaagattt gaaagaaaaa tctcaaactt taacgcccaa aaaagttccg acaacaccaa
     3181 gtacaccaga acaatctgaa atgccaactg ttccaagtgg ctctcaagaa tctagtccta
     3241 cagtgaaggc attccctcaa accggggaga aaacttccaa tgtgctactg ttcattggct
     3301 ttagcttgat ctttgcgacg gctggctatt atttttggaa tcgtcggaac taaggtgata
     3361 agatgaaaca tgtaaatagt agaaaacaat acgcgcgacc gcctcctaaa aaagtggtcg
     3421 cgctattttt gtattcaaaa ctaacaatga aaagaggaaa aaatatggaa ttgaaatttg
     3481 ttgtaccaga tatggtcgag acgttcggaa aactaacata cggtggtgaa ggtgaagttc
     3541 tatcggaagg gtatggtcga aatactacaa ttgttggtcg aagttaccat ctgttttcaa
     3601 ataaacaacg ggccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     3661 ttgaccaaga tcaaccacta aaagctgtaa atccccactt aattgccaaa ggctatgaaa
     3721 ttgagaatcg tgggtttacc gattacgtgt tgtatgttga tgatttagtg aaagcatagg
     3781 aggaaaagaa gatgagatta acagaaggta ttgtcgtaga ttcaggatta acctttggca
     3841 agttgcgttt ttccgcatta cgtcgagaag tacgcaagca aaatgaggac ggaacggtta
     3901 gtaacgaggt aaaagaacgg acctataatt tgaaatcctc cgcgcaaggt cgaatgatcc
     3961 aagtcagtat tccagcaaat gtacccttac gcgagtttgc ttatgatgca gaggtagagc
     4021 tggtcaatcc aatcgtggat acggtcgcca attatgtttt tcgggaagga accaccgtta
     4081 attggtttat caaggcagat gatttagtct tgaaacgaca actgaatcaa ggaaaaccta
     4141 cgaaccaaaa tgaaggaaag aaataggtga agaatatgga aattaatgtt gaacatattt
     4201 tagattgtct cgatcaattt ggcaaaggtg agctaacgga ggaacaactc actaacgcat
     4261 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     4321 atgccaaaga agactttgat gtactggatt ggttagggga agaagagtct ttctttatgg
     4381 ttgtcgaggt aaatgaaagt ttatgtcggc aggcagaatc tgttttggaa gaaatcgatg
     4441 tggagatgcc ggatgccatc gaaagatttt taaaacaatt agtggaaacg aaacaacttc
     4501 ccgtagtagt aaatgactag cataactttt tgaaattgcc caatgaaatc ctattgggct
     4561 ttttcgactg gaggtggaaa ccatcaaaat gtacaaagga catcgcatac gcgcaggaga
     4621 tcaacacttg atttatcact ttgttcttgg ctggctaagt gccttattca tcggttggat
     4681 gggcgtcttt tattttccag agcttagaca atttggtctt tctaaggtat cattttccac
     4741 tattgaaatc gcttggtcca tgaaagattt agtatgtctg cttggaagtc tcgctctttc
     4801 aggaagtatg atgttgcttt atattcattt ttttcctgat cattggcgaa gtttgtggaa
     4861 tcgacaaaag ctagccagaa tgattctaga aaaccattgg tacgaagtga agcaaactca
     4921 aagtgatggc tttttcaaag atttgaatag gagtcgaacc aaagagacta tcagttactt
     4981 ccccaaagtt tattatcgaa tgaaagatgg cttactgtct attcgcgttc aaatttcact
     5041 gggaaaatat caagatcagc tattgaagtt ggaaaagaag ttagaaagcg gactgtattg
     5101 tgagttagta gaaaaggaac tcaaagactc ctatgtggaa tacaccttgt tatacgatat
     5161 gattgccaat cgaattggga tagaagaagt agtggcagaa aatgggacgt tgcgattgat
     5221 gaaaaatcaa gtatgggcgt atgattcctt gcctcatatg ttaatcgctg gtggcacagg
     5281 tggtgggaag acctatttct tactcaccat tattcaagca ctattgaagt cagatgcaga
     5341 attgtttatc ctcgatccca aaaatgcgga cctcgccgat ttaggtacgg taatgcctca
     5401 tgtctattct caaaaagaag aaatttctaa atgtgtggaa gatttttacg aacgcatgat
     5461 ggctcgtagt aggtcaatga aagaaatgcc caactacaaa acaggagaga attatgcgta
     5521 tctcggactt ccgcccaact ttttaatctt tgatgaatac gtggcttata tgggtgcgaa
     5581 tcgttttcct acaagcatcg aatagatgtg aaatggaagc ctattgagat tatcaatagt
     5641 gacaactaat gttttgagag atggaatgaa agccttcaag ttgagctgaa acatccccgc
     5701 taatactcct acatgctcta atcaatgaag tagattggtt ttggtatgaa gctaggtgaa
     5761 gtcgtctgaa atctgcctaa gtgcttgagc ataaggctga ctatagggcg ggcggctgaa
     5821 aaagataact atggtaagaa tgtctgaaaa tgactgacga atctgcgaat gtacgggtct
     5881 aaaaggctga tcttattgaa agataggaac atacggacgt atggtgagat accgacaagt
     5941 aaggttccat gatatgaaaa tcgggatagc taacgaagaa gctataattt cacaggctca
     6001 tagtagatac ctaaggttat acatgatagc taaaaacatt ggaacgatgt aagcagaaaa
     6061 ctatcaatta aatgactact tctagatagg tgcatataag taataacgaa attgctttgt
     6121 ttctgtgaaa gtaagggcac agtaccgatg aagcgtctaa caaatgtgga gggatagccc
     6181 taagtctatt taataacaat gaatgttgaa acagatgaag ttcaaggatc tggtaagaac
     6241 gtgggaaaat ccgttatgga ggtgccacgc ttgaacacaa aaatacgtta ctgggaatat
     6301 tatgatctac aagaaacatt cgatcagcta tttactcaaa gtagaaacgg gaaaaagttt
     6361 tatcaattat atgagttaat catttctgaa aataatattc ttttagcata ccgtactatc
     6421 aaggcaaaca aggggtcttc gacaccagga acggattcct tcacaattga taactataag
     6481 gaaatgaacc aagcagagtt tatccatctt attctcagtc atttagaaaa ttacaaacca
     6541 aaatccataa agcgagtaat gatacctaaa cccaatggtg agaaacgccc attaggaatt
     6601 ccatgcatga ttgatcgaat gatccaacag atgttcaagc agatactgga gccaatatgt
     6661 gaagctaagt tttatgaaca tagctacggt ttcagaccat tgagaagtgc aaagcatgct
     6721 ctaggtcgca ttatgtatct cataaacatc agtaaaatgc actatgcagt agacattgat
     6781 atcaaaggtt tctttgataa tgtaaaccac cgtttactga taaaacaatt atggaatata
     6841 ggaatttgtg acaaacaagt cttagctatt ctttcaaaat ctctgaaatc acccattcaa
     6901 ggagaaggta tcccgagcaa aggaacgatc caaggaggta tcatttctcc actattatct
     6961 aatgttgttc taaacgactt agaccattgg gtctcaaagc agtggcatac ctttgaaaca
     7021 aagtatccct atactaaagg ttacaataaa tttcgtgctc taagagatac aaatctaaag
     7081 caaggctata ttgtcaggta cgcagatgac tttaaaatca tgactaatga ttatccgaca
     7141 gcattaaaat ggttccacgc tgtcaagctt tacctaaaag acagacttaa attagatatt
     7201 tccaatgaga agtctaaaat cgtcaatttg cgtaaatgta agtcggaatt tttaggattc
     7261 gctatttgcg tgaaacaaaa agggaaaaaa tgggtttgta attcacgtat ttctaacaaa
     7321 aaaaaggatc agattaaaga agagattaaa caaagaatca aagatattca aaagagtcct
     7381 acagcacaaa atgctctcct gtttaatgct ctcgtgctcg gtgttcataa ttatttcagg
     7441 tatgccactc atattaattt agatttgaat aggatgaact acgttctttc attcactact
     7501 tacaaccgat tatcaaattg tagtacaaga tgttacccca acaaagcttc cccaacttac
     7561 aaaaagtttt actcaacaac gatgaaaacg tataaagtag ctggagtcta tttgtaccct
     7621 ctttgcgacg tatctacaaa aacaatttac ggatttaata cggaggatac accgtttact
     7681 ccgtttggtc gtcaaagatt ggagcacaaa agcctacagt ctcttgtgtt ccaagaatta
     7741 agaaaattga tggaatcgaa aataccgaat agaacggttg agtatttaga tagtagaata
     7801 tctcgttact caatgaaatc aggcaagtgt gagattacaa aacaattttt gccagcaaaa
     7861 gccgttcact gtcatcacta tttgccaaaa agtcttggtg gagacgacaa attcgataac
     7921 ttgagaatta ttcataagga tatccatctt ctgatccata caacaaataa aatgataatc
     7981 gatcactacg tgcatgaact gaaactggtg ccagagcaaa tagcaaaaat caacctttat
     8041 cgaaagatgt gtaatctgca aaacattcaa tagaaaataa atagatggaa cgccgtatga
     8101 ggtgaaagtc tcacgtacgg tgtggagcgg gggaaaagat ggagattata tcaaattctt
     8161 acctatcgct atagatgtta acgacaaaag aaagtgcggt gattttgaat aagcttaaac
     8221 aaatcgtgat gttaggccgt caatctggtt tctttctgat tctagcttgt caaaggccgg
     8281 atgcgaaata tctaggagat gggattcgcg atcagtttaa cttccgtgtg gctttgggtc
     8341 gtatgagtga gcttgggtat tccatgatgt ttggagaagt cgataaaaat ttttttatga
     8401 aacgcatcaa aggacgaggt tatgtagata cagggggcag tgtgattagt gagttttata
     8461 cgccacttgt accaaaaggg tatgatttct taagggaaat tagcaacatt gttggattaa
     8521 ccgttcatac ggaaagagaa aataatagtg tgtagcctag gaatatcaat aatcctgggc
     8581 ttttttattg aggtgattta tgacattttt agatttattc gccgggattg gtggctttcg
     8641 cttaggtatg gaacaagccg gtcaccagtg tatcggtttt tgtgaaattg atgaatttgc
     8701 tcggaggagt tataaagcaa tccatgatac aaggaaggag gtggaaatgc atgacatcac
     8761 aagtgtatca gacgaatttg ttcgatccct cggaccagtg gacatccttt gcggcggctt
     8821 tccgtgccaa gctttttcaa ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac
     8881 tttattcttt gaaatcgctc ggttcgccac tcttctccaa cctaagtttc tattccttga
     8941 gaacgtccgg ggattgctca atcacgaagg aggggctacg ttcgagacga tcctccgaac
     9001 gttggatgga ttggggtatg atgtggaatg gcaagtgctt aactcaaagg cctacgttcc
     9061 ccaaagccgt gagcggatct tccttatcgg acattctcga gatgcatgta ccgaacaagt
     9121 atttcctatc attggatcgt cttcaacatc tgatcaaaac atcagaaact tggtaaatat
     9181 caatccttcc aatcgaggaa tgggtggcca agtctatggt tcagatagcg tagcaccaac
     9241 cttgaccggt gatgaaggaa ttaaaattgc cttaccggta aaggatggga tttctgtagc
     9301 gggtatgttg tcgggaaatt ttgaacaagg caataggatt tatgaggtaa ccggaacagc
     9361 ccccactttg tcaacgaaac aagggggaac gaagattatg attcggaaaa atgaaacaga
     9421 atatcaagag gtgaagccag gggacagtgt gaacctggct tttcctaatt ccacaagtcg
     9481 ccgaggtcgt ctgggaaaac aaagtgtgca taccttacta acaggagacc aacaagcggt
     9541 agtcacggat cagtaccaga ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg
     9601 ctttccagat tgggcttttg atcgagcttc tcaagtcaat tcagatagtc agttgtataa
     9661 acaggccgga aactcagtaa cagttccggt catttttgat attgctagaa gattaaaggg
     9721 ggtaaaagca tatgatttat gacgagttga ttggagaaat ctactgggtg attggcaaga
     9781 ttcaggcaga tccgaaattg gaagaagaac tgtaccggct caattttgat attcgcaaaa
     9841 acggggtgaa agtccaggtg attcgtatgt agtggatgag gaaacggatg ctcgaatgga
     9901 gctcaatcaa gtcatcggtg aattggaacg aattgcggac cttgccaaag aacctgatat
     9961 ccgccaatac ttgtttgaga taaaagcaga gttggaaatt gagggtatca ccgcggagta
    10021 atagggaaat aacccactga taggcactga ccgcggggga tttgcgaagc agaaatcctc
    10081 cgtggtgctg tgcctgttca tggacggcgg cagccggcca tgatgtctca ccccccgtat
    10141 ctaacagggg ggtacaaaaa cacaaaaaac aagcaagtaa ccataaaaaa tgcttgttgt
    10201 atcaaggata ttaagtaatt tgtataggtg ttaactcatt tggaaaagtt aacaccttga
    10261 atttggaggt gaaaacttgg cgcaacgaaa tttagattat cggttattaa aagaacgacg
    10321 caacgaatat ggcgtttcac aaaataaatt agctactgct tgcggactta gccgcccata
    10381 cctaaatcag atagaaaacg gtggcgtaat cgcctctaca aagaccatga gaaaaatttt
    10441 tgatcaactg gaaagtttca atcccgattt acctttaacg ttactatttg actatgtaag
    10501 gattcgcttt cccacaacgg atgtgcggaa aattattcaa gaaattctcc atttgaaatt
    10561 cgagtatatg cttcatgaag attacgcctt ttactcctat caagaacaat atgtcatggg
    10621 cgatattgta gtgatgttat ctcatgaaga agataaaggt gtccttttgg aattaaaagg
    10681 tcgtggttgc cggcagtttg aaactttttt actcgcccaa aagcgtagct ggtacgactt
    10741 ttttgaagat tgtctaaaag ccggtggcgt gatgaaacgc ttggatttag caatcaatga
    10801 tcgagtgggg ctactggata ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat
    10861 ctccttgttt cgtaccttca aaagttaccg ttccggggaa ctgttgaaag ctgatgaaaa
    10921 ggatggtatg ggcaatacct tatatattgg cagcctcaaa agcgaggttt atttttgttt
    10981 gtacgaaaaa gattatgaac aatatatcaa gttagggatt ccattggacc agaccgaaac
    11041 gaaaaatcgt tttgagattc ggttgaaaaa tgaccgtgct tattatgcga ttcaagattt
    11101 gttaaaaggt cgtagcattg aaagtaccac gttttccatt atcaatcggt atttgcgatt
    11161 tgcggataaa gtagatggaa aacggcgaac caattggccc ttgaatgaac aatggggtcg
    11221 ttttattgga cggaatcgga aagagattca attaacttct gaaccgaaac cgtataccat
    11281 tgaacgaaca ttaaattggc taggtagaca agtcgcccct acgtggaaaa tggctaagga
    11341 gttggatcga ttgaatcaaa caacgtatat ccaggatatg gtacagaatg cgcgactctc
    11401 ggaccggcat aaaaagattt tggagcaaca aagtatggca atcgaaaact tgattgtatg
    11461 aaaagaggaa ttttgatgga aattgtaaaa gatatgttat tggtacttgg tggcagtatg
    11521 cttggtgtta ccgtgatgtg cttgatgaat gcaagtgcag cagcggattg agcaatggaa
    11581 aagaaaggaa agtgacaaaa tgaatttcgg acaaaattta tatcaatggt ttttaaccaa
    11641 tgctcaatct ttagtcttac tagcgattgt ggtcattggt ttgtttttag gatttaaacg
    11701 ggagttttcc aaactgattg gcttcttggt gattgctttg attgcggtag gtctggtctt
    11761 taatgcttct ggtgtgaagg atgtcctact caatttgttt aaccgaattg tcggtgcctg
    11821 agggggaatc acaatggaac agatgcgtgt ttatattgcg aaccttggaa aatacaatga
    11881 aggtgagttg gtgggggcat ggtttacccc acctgtcgat tttgatgaag tgaaagaaca
    11941 gattggatta aatgacgaat atgaagagta cgcgattcat gattatgaac tgccttttga
    12001 gattgatgag tatacgccaa ttgaagagat taatcggttg tgtaacttag cggaggaact
    12061 agaaggaacg ccaattggtg aagctgcttc tgagattcaa cacgctttct tcaattcttt
    12121 cgaggaaatg gtagagcatg tggatgatat tgtctattat ccagattgcg atgatatgag
    12181 tgatgtggcg cattacttga ttgtagaagc tggtgatttt ggcgaagttc cagaacaatt
    12241 gaagccgtat attgattttg atgcttgcgg tcgagatttg gagattggag gaaattattt
    12301 ggttactagt cgtggagttt ttgagtatcc agaatgaatc aaacactata tatagtgttt
    12361 gattagtata tgtaagcaaa aaaacacaat atatagagtg caaactgaag ggtaatgtga
    12421 tataatattt gccgaagaaa tatttttgaa atgaggtaat attatggcaa atattcaaac
    12481 aagttttatt gatttccata attcaattag attggacgta gaagacaata cgctacttaa
    12541 ggactataaa gaccaagtaa ttgatggatt aaaagattac ttacctgatg atgtgaagtt
    12601 tgaaactttt ttacagggaa gctattctgt ttataccgga ataaaaagct gtgatgaaaa
    12661 aattgatttt gacatcgata ttgcagttgc tttcgaaatt gatcatactg tttatgaaga
    12721 tccaagggag cctaaactgt gggtaaaaga ggcgctagtt gaaatttttc ctaatgctca
    12781 agttaattta aaagtacctt gtgtaactgc tacatttact ggaaaaaaga caaaaaaaaa
    12841 tgtgcacgtt gatgttgcgg tttatgctaa agaagatgaa aattatttct tagcaaaagc
    12901 aaaagaattc agtgctcccg aaaaccgatg ctgggaggaa gcggatccca aagtattaaa
    12961 agagaaaatt aatagtcatg tagcagattc agatgatcga aaacaatttc gaagatgtat
    13021 tcgctattta aaaagatgga aagataacaa ttttaaccaa gaatataaac caacaggaat
    13081 tggacttaca attaatgtga tggatacgtt cctagttaat aagtccactg attttttaac
    13141 tagaaaagtt cagtataatg atatggaatg tatgaagcag attgtttctt cgctgaaaga
    13201 ttcatttgtg tatgagtaca gtgaaacaga tggttggcat tatcgtctac atgcatatct
    13261 tccagtgaag cctaacagtg acacctattc aaaaatgaca gtgaatcaga tgagtgattt
    13321 taaaaacaaa ctttctaaat tatatgatga tttgattttc gctatcgata ctgaagatga
    13381 gtatgaagct acaaaacgat taaataatca atttggggag gattttttaa ttatttcaga
    13441 agaagaagtt acagaaaaaa acttaagaaa tgcatttgtt acagactatc cgagtgctta
    13501 ggttggtgtt aaagattgga ttatactgat attttaaaag aaaagctaac agatgttgtg
    13561 cgtttattcg atggtaatta ctatggggat ttttcatttg ctacaattgg aaagttgcgt
    13621 ttaaaaattg aaataccaaa acattttcca ttggtacttc ctaggattta cattcataat
    13681 tttgatcatt taggacttta cctaccacat gtagaaaaag atgggttggt atgtttttct
    13741 tcagatataa attatttagt agatgaagaa aattcagaat tggttttatt gcagtgtgtt
    13801 aataaaacta taaaaacaat tgaggaaggt ttgaaagggt taaataaaaa tgattttcga
    13861 aaagagtttg ttgctttttg ggacaaacaa gagaatcaaa tttcgttatt ctacctttgt
    13921 gaaccagcgt tgtttatcaa gtcagttgat acaataatgt acggtaagtc tttggttgct
    13981 ttggataaag agaacgaatt acaattaaat gcagcgagta gattgtttaa tggagagaaa
    14041 gaagatactc atgaattaga tgcatactat gttccaatta gaaacaagaa taatattatg
    14101 cccccgaaga ataataaagc atggtcaaag aaggatattt tacaaatagt aaaaaaaaat
    14161 gtaacaggtg caaataaaag atttttttta aaacttgaaa aaagaaagaa tagacataat
    14221 gttttactta ttctagatat cccaattgaa gaaaagtcaa ataatgtcat aattggattt
    14281 ctttttacaa aacaatctgg atctataaaa gattataagg gtacgcctag gactgtatta
    14341 gttcagcgtc atgatgaatt atatttggtg aatagaacga gcggagaaaa ctatggattg
    14401 ggtgctaata tagctattgt tggggtaggg tcagttggca gtaaaattgc tgaaaatctt
    14461 ggtccattag gtgtgagaaa gatgacactc atagacaatg attgttttca agctgaaaat
    14521 ttgtatcgaa attcacttgg tgcagatagt ttaaaaaatg cccgatctca gaataatttt
    14581 tttaaagttg atttaatgca ggaggagctc gaaaaaaaat tcccatacct ttgtgttgaa
    14641 gtggagtcaa tgaacgtttt agacttaatt agtatgcaac ctggtttttt caaggaatat
    14701 gactatgttt ttgtttgcat aggagataca atggcaagcc tggctttaaa taattatttt
    14761 tccaaaagta gtcaaaaagt tttctattct tggctggagc catatggcat tgggggacac
    14821 actctattta taaattattc tctaaatcaa aaaggttgtt tcaattgtct ttatacagat
    14881 tcaaactcta aaaataggtg ttcaaataga gcttctctgg taaaagaagg acaaattttt
    14941 gaaaggactt tagcgagttg ccgctcaaaa tttataccat ataatgctat tgcctcgacg
    15001 gaatgtgcaa ttaatgcaat aaaacttttc cataaagtca atcaaaataa tatgaccaat
    15061 agtactcttt tttcatggat aggagatggg agtacttttg agaatttagg atatcaatat
    15121 agcgacagaa atacttttct taatggagtc aataagtttg aagaaagatt cttttctaaa
    15181 tattgcaaag tatgtgggga gttagatgat taaattttcg tgtgaccgta ttgtcattga
    15241 agtaagggat gaagttctta agatatttga acaatatcgg cagggaaagg atgacttgcc
    15301 tgaagctgga ggagttcttc atggaaaaag acttaagaat gggagcataa taattaccga
    15361 tataacaaca cctcaaatag gagatataca aaaaagatta ctctttaaaa aaaaccgaga
    15421 aattcatcaa cagctttcta atgagaaatg gatagaatca aataagtttt ctattgcatt
    15481 gggagaatgg catactcatc ctgaagctgt accaactcca tcaaatgttg ataagaaaag
    15541 ctggagactg aacgtgagca agcaacatga tgatagagtt tatgttttta ttatcgttgg
    15601 attttctgat ttgagaatgt ggttactgtc aaaaaaatca gatattaggc aggctataaa
    15661 actataaagg agtgattagt tgaaatcaca aaagaatatt tggtggtggg ggtttttagt
    15721 aggaaaggta atgataccag cccttctctt aatttctcta ccgatgatta cttttttctt
    15781 taccaataaa aaaatgagta aagaagccgt gatttttttc tttggagacc aaaaagctac
    15841 ctttattgaa acgttagtaa catctcttca gcttaatttg aattacattt tttcaattat
    15901 tattgttata tcgttattga atttttttaa aaaaagaaat tcaggaaagg tgtttaactc
    15961 taatggaaat gtgtactaca actattttta ttttgtattt tgggtagcag caactttact
    16021 tggttatgat aaaattcaga ttgcaggaat tcctatacac atgcaatata agttggtttt
    16081 gagtggaatt ttttctgagg tgttgccaga tatatacgat gatcattacg acagtgatgg
    16141 aacatgtaaa gtaagcattg aaaaagaaaa tttcgatgac atcgatggat atgactctgt
    16201 gaacttactg attattgata cttacgatat aaaaatgagt gaattatcta tggaaaatca
    16261 aacatatcct acgataattg ttagagggaa ttcaatagat ggagttcgta aagttaatag
    16321 atctttaatc ttggaaataa aaaaaacaat ggatgaaatt caaaaaagtg actttaaaaa
    16381 agtatttgtt gcttcaacat ctaatccaaa aaatagtatc aatattatta attcatcgtt
    16441 tagatttttc ggtcgatcac gtaggtttaa actttatgtt cttcaaaagg attatgcaag
    16501 caatggaaag tatagtaaga aatatagaat ttttatttaa gaaggaattt ttccttcttt
    16561 ttattttgga ggaaactatg aaaaaaataa aaagctacac cagtatttgg gcagttgaaa
    16621 aagtcattta tgcgattaat gactttcagc tgccttttcc tgtgaccttt aaccagatgg
    16681 cttggtttgt tttatccttg atgtttgtca ttgtatttgc ccatgtgcct ccgctatcca
    16741 tgatagaggg ggctttttta aaatacttgg gcattccgat agcggtcact tggttcatgt
    16801 cgcaaaagac ctttgacggt aagaaaccat tgggattttt acgatccttt atcagctatc
    16861 acttacggtt taaggtcact tttgcaggaa agaaagttaa agaacagaag aaacgatggg
    16921 acgaaccaat caccttggta aggagtgtga attatgtacc cgattaagta tattgaaaat
    16981 aatcttgtct ttaaccaaga aggcgagtgt tttgcttact atgaattagt gccttacaat
    17041 tactcctttt tgtcacctga acagaagtat caagtacatg ataactttcg ccaattaatt
    17101 gcgcaaaatc gtgaaggtaa gatccatgcc ttacaaattg ccacagaaag tagtattcgt
    17161 gccacacaag aacgttcgaa aaaggaaatt accggtagac tcaaagaagt agcaaagcaa
    17221 cgaattgatt tacagacaga tgcattagta tccatgattg gcgatagtca gattgattat
    17281 cgcttcttta ttggctttaa actgatttcg acagatgaag aggtcaatct aaagaatctc
    17341 aggaaatctt tcttttctgg acttcaagaa tttgtctatg gagtgaatca tcatttgatg
    17401 ggtgattttg tttccttatc caatgaagaa attcgtcgct ataccaagct tgaaaaacta
    17461 atggaaagta aattggctcg aagatttaaa gtcaaacgag taacgccgag tgacttgacg
    17521 tatttaattg aacatatcta tggtgaaaaa ggcacaccgt ttgaagaata tgaattccag
    17581 ctaccaaaga aaaagctgaa atcagaaacg ctggtgaaac gttacgactt attacgtccc
    17641 actcgttgtt tgattgaaga aaagcctcgg tacttacgga tggaacatga aaatcatgag
    17701 tcttatgtag cgtacttaac aattaatacc attgtagggg aaatggagtt tccttcctcg
    17761 gaacttttct attaccagca acaacaattt acgtttccga ttgatacttc aatgaatgtg
    17821 gaaattgtga cgaataaaaa ggccctagct accgttcgta ataagaaaaa ggaattaaaa
    17881 gatttagaca accatgccta tcaatctgat aatgaaacga actccaatgt attagatgct
    17941 ttggattccg tggatgaatt ggaaaccacc ttggatcagt cgaaagaatc catgtataaa
    18001 ctaagctatg tggttcgagt gagtgctaaa tcagtggatg aattaaaacg ccgctgtgat
    18061 gaagtgctcg atttttatga cgataccaat gtaaaactag tccggccctt tggcgatatg
    18121 atgggtctac acaaagaatt cttaccctca agtaagcgat acatgaatga ctatattcaa
    18181 tatgttacgt ctgacttttt ggcgggactt ggttttgggg ctactcaaat gctaggagaa
    18241 ttagaaggca tttactttgg ctataacgta gatacggggc gcaatgttta cctgaaaccg
    18301 gcattggcat cacaaggggt caaaggctca gtaaccaatg ctttggcatc tgctttcctt
    18361 ggttcgttag gtggggggaa atcttttagc aacaatctct tagtctatta tgccgtcctt
    18421 tttggtgggc aagctgtgat tgtcgatcca aaaggagaaa gaggcggttg gaaagaaacg
    18481 ttgccagaaa ttgcggatga aattaatatt cttaacttga ccagtaagcc agaaaatcaa
    18541 ggcttacttg atccctatgt gattatgaag aaaaagaaag actcagaaag tttggcgatt
    18601 gatatcctga cctttttaac agggatttcc agtcgggatg gggagaagtt ccctgtgtta
    18661 cgcaaagcca ttcgaacggt tacccaaagt gatcagcgag gattattaca agtaatcgtt
    18721 gaattgcgta aagaggactc accagtcgcg gaaaatatcg cggatcacat cgagtccttt
    18781 accgattatg attttgccca tttgcttttc tcagatggtt cgattaccca gtcgattagt
    18841 ttagataagc agttaaatat cattcaagta gcggatttgg ttttaccaga tgcagaaacc
    18901 acttttgaag aatataccac catggaattg ctgagtgttt ccatgctaat tgttatttcc
    18961 acttttgcct tggactttat ccatagcaac cgagaaattt tcaagattgt ggacctggat
    19021 gaggcttgga gttttcttca agtagctcaa ggaaaaacgt tatcgaataa attagtgcgt
    19081 gctggtcggg ccatgaatgc cggtgtttac ttcgtcacgc aaaacagtga tgacttgttg
    19141 gatgaacgtt tgaagaataa tattgggcta aagtttgcat ttcgcagtac cgatattcat
    19201 gaaatcaaga aaaccttgga attctttgga ttagataaag aagatgaaag taatcaaaaa
    19261 tgcatgcgag atttggaaaa tgggcaatgc ttgatgcagg atttgtatgg tcgagtaggt
    19321 gtggtccaag ttcatccagt ctttgaagaa cttttccatg cttttgatac ccgtccgcca
    19381 gttcaagaga gaatggagtg aggagatgaa aaagaaactt tttcgtatct ttggaattgt
    19441 tttaatcagt ttggtcactc tgataatact tttgagctta gttggaacgg tagctgaggc
    19501 aacaggtctg gttgatgata cagttggagc cggaaatctt tattcccaat attcacttgg
    19561 taactatcag ttggatttct ttgtggatag ttcgtgggat tggctgcctt ggaattgggg
    19621 tgatggccta gggaagagcg taatgtatgg actttatgcc attactaatt ttatttggac
    19681 agttagtctc tacttatcaa atgccacggg ctacgtggtt caagaagcct ataagctgga
    19741 tttcatttca gatacggctg aaagtattgg gaagaatatc caaaccttag ccggcattac
    19801 cgaaaatgga ctccagacct cgggctttta ctttgggttt ctattattga tgattttagc
    19861 tctaggagtt tatgtggcgt atacaggatt attaaaacga gaaactacca aagcagtccg
    19921 ggctgtcatt aattttgtga tgatttttct actttcgggt tcgttcattg cgtatgcgcc
    19981 cacctatatc acaaaaatca atgattttag ctcagatgtc agcgaagcgg ctcttagcct
    20041 tggaaccgag attgtcgtgc cgaattcaga aagccaagga aaggacagtg tggatttaat
    20101 ccgagatagc ttgttttcca ttcaagtcca acaaccgtgg ttactcttgc aatttgatga
    20161 ttccaatata gaagaagttg gcgaagatcg tgttaacaaa atcctgtctg taagtccaga
    20221 tgaaaataaa ggcaaggatc gggaagaagc ggtcaaagcg gagattgaag ataatgacaa
    20281 tgcgaatctc agtattacca agaccatgaa ccgcttaggg accgtggtct ttttggtgtt
    20341 gttcaatatt ggcatatcct tctttgtctt tctcctaaca gggattatgt tgttctcgca
    20401 aattctcttt attatctttg cgatgttttt gccgattagt ttcttattgt ccatgttgcc
    20461 gacttatgag agtttgggca agaaggcgat tattcgcttg ttcaatacga ttatgatgcg
    20521 agcaggagtt actttggtga ttacgactgc atttagcatc tccacgatgt ttttcaatat
    20581 ttcggcaacc tatccatttt tcatggttgc ttttctgcaa attgtgacct ttgccggtat
    20641 ctatttcaaa ttgggcgaca ttatgaatat gttcaatctc cagagtaatg atagccaatc
    20701 aatgggaaga cgggtcatgc gaaaaccgca aatgttaatg aatcgaacat tgcggcaact
    20761 caatcgaaat gtgggacgga ctttggcttt tggtggtggt gcagcagttg gaaataagtt
    20821 ggcaaaggaa caatcaaaat ccaaatttaa gccatccggt tcttctctac gaaagaattc
    20881 acgattaccg aacgatcacg agataccatc tgacttgaat aaggaaaagc caattccaaa
    20941 taataagaaa caatcccgaa tgaatttggc cgggagaaaa gtcggcaagg ttctggatac
    21001 ccaagctttg gtgaaagata agaccaaaca agtaaaggat ggagtacgga atacgccaac
    21061 caacttgaaa tacaacttcc ataaaggaat tgaaaaaacg aaaaaagcac cggaagagtt
    21121 taagcgagga ctggtccaag aaaaagcaaa tcgaggagag ttgcgagaaa aacaacaaca
    21181 acgtagagac gaaaaaatag ctgaaaagcg caaagtattg aacgaaatag ggaatcctca
    21241 tgggaaaaga agggaaaatg ttccgataaa agcaaaggtc caatctcaaa aggatagagc
    21301 accaaagcgg attgtgagag caaattcgaa tcccgaaatc aaacgaaaat tcgctagcca
    21361 agaaatcatt tcaaaaggaa taaaccaatc ctcaagtaag aaaaattctt ttcaacaagt
    21421 gaaacggcgg tctactctgt ccaaacgaac caatcgaaaa gtacaaaaaa gtttgaaccc
    21481 tcatcccaaa ccaaggtcag gtgagaaaaa atgaacgtaa agaaattaag tgccatttcg
    21541 ttaactttcc taacaatctc ttttatgggt atcttgatgt gtgtgggctt attgtttggt
    21601 gaagacagtg aaggaagtag tagttcaggt gctcctcctg gggggagtag tgtctcagaa
    21661 gaggttttga aacaccgtgc aatggtggaa aaatatgcca aagaatcgga tataacagag
    21721 tatgttccga ttcttttagc gattattcaa gtggaatctg gtggcacaat ggaagatgtg
    21781 atgcaaagtt ctgagtcagc cggtttacca ccgaattcat taagtaccga ggcatccatc
    21841 aaacaaggat gtctctattt tgcgtctttg gttaagcgtt ccaaagaatt aggttgtgat
    21901 caagatagta tcattcaggc ctataacttt ggcggtggtt atttggacta tgtggcaaaa
    21961 aacgggaaga agcatagttt tgctttagct gaatcttttt caaaggaaaa atcttgcggt
    22021 caaaaaatcg attaccctaa tcccatcgcg aataaggaaa atggtggatg gcgctataac
    22081 tatggcaata tgttttattg cttattggtg aagcaatacc taactacaac acagtttgac
    22141 gataaaacag tccaagggat ttttgacgag gcgtttaagt atgaaggaac ggcttatgta
    22201 tttggtgggt caagacccga aacaggattt gattgtagtg gcttgactca atggtgttat
    22261 gccaaagttg gtctgaaact tcctcgggtg gctcaagatc agtatgatgc catgacccat
    22321 attgacctaa aagatgccaa accaggggat ttggttttct ttcattcgac ttatgatgcc
    22381 ggtacttatg tgacccatgt cggtatttat gcaggggaga atcgcatgtt tcatgcagga
    22441 gatccagtag gctggacgaa tctaacagaa agctattggc agcagcatct tatcggtgct
    22501 ggacggtaca aataagagaa aagaggaaaa agaatgaaga taaaaattga acggaatcaa
    22561 aaggaaaaga gcctaaaaaa gaaaaaagaa cgagtcgtat ccgtgggtaa gcatcgaaaa
    22621 atggtgcttg ccctttggct tttattatct tgtagtctta cttttggcgt ttataaaaat
    22681 ttcaccgcca ttgatcaaca tacaactcat gaaaaagtgg ttattaaaga gaaagtagtg
    22741 aatacttctg gtattgaaaa ctttacgaag gatttttcga aagaatattt ttcgtggaaa
    22801 aataacaaag aagcaattga aaagagaatg aacaacctcg aacagtacct tacagaagaa
    22861 ggtttcgctt taagtcaaga tatggttcga gcagatattc ccactaattc cgaagttcag
    22921 tcagtaaaag tcttagatgt tgaaaagagc tcggaggaat ttgtcgtttc atttttagtg
    22981 gagcaaaaga ttacagaggg aaaaaaggca caatcgattt cttctgcata ccgagtgacg
    23041 atctttgaag atgaaaacag gaattatata gtaacgagtt tgccaactat gattgcaaaa
    23101 cctgataggg caaaatacaa gacaaaacaa gtagaaaatg attcgaaaat tgatgccaaa
    23161 actacagaag aaatcgctga gtttttggaa acgttcttta aattatatcc atcagcttct
    23221 gaaaaagaat tggagtatta tgttgaaaat gatgcgatgc gaccaatcaa tattgactta
    23281 aagttttcga agttagagga tccagtttac ctacaaaacg gaaaaaaaat tctagtgagt
    23341 acaaagataa aataccttga taatgtatca aaaaccacta gctactttca gtacaaatta
    23401 atattacaaa aaaatggaaa ctggaaaatc attgatgtta aataataatg actcaatcta
    23461 aatttatgag aagttataag tagtttaact tatttcaggc agaagtcgcc catcctctat
    23521 aggtgggtga tgaatgccgt tcggtaactc gaaaaccgac aattaagaca tagtgccttt
    23581 ttattttaat ccatgtcata atcagtctat ataagaaatg gttgatatac catggaatta
    23641 gatacaaata atcattcatt atttcttctt tgttaccacc ttattttagt agtgaaacat
    23701 tgtaaacatc tcattgatga tgtaatgtta gcttatgcaa aaaactatct ttgaaagaat
    23761 atcagaatca catcacatta ccttagttga gtgaaatcgt gataaagact actttcatat
    23821 tatgctcaaa gcacaaccta aaaccgaatt aacaaaattc agcaatgcct aagctcatgt
    23881 ctgataaaat gagactttct aagggtcaaa aatttccttt gaatacgatc attagcttag
    23941 ataaaagtaa aaaccttaga actacgggga tagcttggtt aataagagat atcgctgtta
    24001 ataaagacat aagcttgcaa gtatgctcta ttcccaagaa tctcccactt caagcactaa
    24061 ggatcgttaa attcagctaa gtggcaagta gttcactaga cgataattat ctccatattt
    24121 caatctcagc ttttccattt ttcatagcaa attccgttat ttttatagca tctaaaagat
    24181 tcagcttttc cactgatctt tcaccacttt tcagcttttt tattgtgctt aaattgatac
    24241 cagtaccttt tgatattgaa ttggaagtaa aatcattaag ggtttttttt attaaattca
    24301 tatccgcaat tccagcgttc ataagaatga agattctcct ctcatagact actttgaaaa
    24361 tggtactaca gtggctgttt atatatttca gtaggttact gtagtggtca ttttattata
    24421 attagaaaag gaacctaaat caaaaatgct aaagaaaatt ttcatagtat taaacctatc
    24481 atgactaaag ccacggaatt cttgataaaa gagtatactt gcaagcctat gtctttacta
    24541 acagcgatat ctcttattaa ccaatctatc cccgttgttc ctaaggttct tgatgttttc
    24601 taaggcaatg cttgtataca tagcccttga tcaaaatatt gatactaacg ttgacgtttc
    24661 gatcatgatg tgtacggcaa ataggacaag tccattctcg aattgtgaat gacttcttac
    24721 catctgtgtg tccacattct gaaccaattt gactagacgg aaatcattta tctatcttga
    24781 tgattttttt gacagaagta tcgaaaatct ttacataatc tgggtgttgc attcttatgc
    24841 agatgagagc gttagctccg tattcataac cagttgcctc taaaacgtag tccatcggtg
    24901 gatacctagc ttgcggttgc cagtggcttt cgcagtcttc ttgtaatcaa atcgagcatc
    24961 taattgagta ctagtatttt gaagataata cttgagtttt tcggttttca gaagttcact
    25021 tcatcttcca tcatccatgg aaatcccact cacccatacg caggcctcca aaaattcttc
    25081 aacaatggtt tttcagacat ttttctgaat acctccttgt tttttgatac gccagatagt
    25141 caggaaaaag gcaacaaaac acaaaacaaa gctgcctaaa aaggtaatct tcattccata
    25201 taaaaagaca tccggccgat ctgcgatata gttagttact cgttggccca tcttagcaga
    25261 catcgcttga tataagaccg tcgtagaaag cgaaatcccg atgaccatcc ccaaatttcg
    25321 ggcaaaggaa ttcatggagc ctgccagtcc caaattttcg atagtgacag aactcataac
    25381 catcgtatta tttggggact ggaacaaggc attgccggca cccatgatgg cagtagcgac
    25441 aatgtacagc cacaaaggag agtccactga caaaaacata tacatgactt gggttaccga
    25501 caataaaccc aagcctacta atacaaaaat ctccgggcca acccggtcgg tcaggtatcc
    25561 gcttaaaggc gccccgacga ccatcatcaa gggaaagacc atcatcaaca gtccggcata
    25621 gctaggactc aacccgcgag cattttgcag ataaaaagga atcactacat taacaaagaa
    25681 gttggaagaa aaaatcaaca aggctgtaat caagctcatg gtaaaaatct tgttcttaaa
    25741 aataccgaaa gaaatcaatg gagaggctgc tttcttttca acttttaaaa agctgacaaa
    25801 ggcaatcact gaaattacga acagtacaag ggggactgga tggaaaaagc cactttcttg
    25861 ccccaaaaag attgttccaa aaaggctgat aatcgtagcc gcaatggcag caaagccccc
    25921 tccatcgatt gtatggccgc tcatggtgat atcttttggt aagaattttt cagcaatcaa
    25981 catcgttacg attcccaccg gtacattgat ccaaaaaatg tacgcccaag aaaattggga
    26041 caaaatcaag ccgccaagac ctggtccagc aatcgagcct aatgaaacaa gcgcaccgat
    26101 tgctcccaat gcccgaccac gttcatgtat gggaaagact tccgtgatga ttccggcatt
    26161 tgtagccata gtcatactag caccaatggc ttgtaccacc cgtgcgaaca attaaatacc
    26221 tagcgaatga ttgaacccac acaacagcga gcccaagaca aagatcagcg taccaatccg
    26281 aaatactttg attttcccac agctatcgcc aattttacca aacagcaaca aacaagaaca
    26341 gacaatcatc agataaacgg acactaccca ttccgcttga ttcattggta tgcccatatc
    26401 atctgagatg gtaggtagtg caatattgac aatactgcca tccaatgtgg acatgaaggt
    26461 aaacatagat atagcaatca aaatccacca gcgatttttt tggacttttg catcttcttg
    26521 ataactatgc ataataggat ttcctttcta atgatcgtgt agctaagaac aatataacct
    26581 atgtctactg cccaacaact tctcgcgact ctagccatag gaagcttgag taatttcgat
    26641 ttgttattct catgtaacca tgtaagtttg agatgcttta tttgaactta ctctatagta
    26701 actagtgata attcgcttta ctttcttcta gcaatttcta aaagtcgact tcattgttct
    26761 tcagattaat agtgtgagct atctcgtgag acgtaagaaa atttccattc tactttttcc
    26821 caattttttc atatttatgg tgttgcacct atttgttttc caatttgctt ttcaaataca
    26881 ttgctatgat attcaattcc ataggattat actaaatgtc aatagtatga ataatatatt
    26941 atagatgttt atatatcact gttaattatt atccacaata attaatgatt acctcgatat
    27001 ttgtcaatca aaggttgaaa caatttattt tcagcatctt ttctaacttg agctgcactc
    27061 ttaatagaat catacgatcc taaatagtac ctcttttttt taaagttata tacgcaatcc
    27121 atttctcttt agaacgataa actcccttaa caccacttgt attatttttc ggtaattttg
    27181 acgttagaca ctctattact gtcccttcaa cacatgtcct tgtttcttgc ctttttaaac
    27241 atccacaact ttgagtatgt ccaaaaaata agttactata acttatctca cacatactgc
    27301 cacactcaca ttgacaaacc cattttgttt tccatgaaca gttattgtcc aaaggatact
    27361 ttgcagtaag taatccgaat tttttatttt caatgtgatg cttttttgat ttcaaacatc
    27421 cacagctttt tacatcacct tttagaagtc gctttgccga tactaacttt tttctcccac
    27481 acagacattg acattcccaa acaacagttc cttcacttcg cagtttagtt ggcttgattg
    27541 caactaactt accaaaagtt ttatttgtca aatttttata tttatgccta tttttataat
    27601 ttccacatcc acaacttttg gttctacctg atagtaagtt tgatcgatat acaatcgttt
    27661 cattcccaca gtcacatagg taacgatatt ttttatatcc attatgagta atctcctcaa
    27721 caacagtaag gtgaccatat ctttccttca ctgcacctac cctcccaaga aagaagtacc
    27781 atataatttc aattaacttt taataaatca gaaaaaaata ttttcaattt ttcttagtct
    27841 gattttatga ctaatttata atacaagact gaacgatacg tacacgaaat cctcaagata
    27901 gataaattgt tttcatctag taaaacctgc tcaaaatatg agcataaaga cggcaataat
    27961 acactcgata ttcgataatg gacttgtttt atttgtcaaa ctcatcgtaa tcgagatatc
    28021 aattccagtc tcaatatcct gatcaaaggt ctaagaatcc aaccattttc ttgttacttt
    28081 gtcagattga ttaccttttt tagtctccga ataataaggt tttcggatca actaacatgc
    28141 tctgagaaag actactatct ttgcaaattt ccaaatgaag gtgtgggcca gtactattcc
    28201 cagtacttcc tacatagcca attatttgtc cttgcaaaac attatctcca gtttttacta
    28261 ggtattgttg ctggtgagca tataacgcgg ttgatccatc ttcgtgttgt atcaccacgt
    28321 aatttcccca agagtagtga aactctgctt taacaacctt accagcttta actgcatgaa
    28381 tcggctcacc ctgtgtagct gccatatcaa gtcctcggtg aaattcattt ccacgatttc
    28441 caaacaaact ggttattgaa taattatgaa taggcacttg gtatccagtc ttactcattt
    28501 gtggaccttt aacttcttta tcatagttgg ttaaatcata ggttttaatt aattcattta
    28561 gcttcaaata gtattgtgta tctgttgcat accgtcctgt aaggaattta gttgcttctc
    28621 gatatgtttt actattagct tttaatgctc ctgcataaaa atctttattt ccactaattc
    28681 ccttagttaa taatttggca taatcttcaa aactttcctc atagttttca tacactctaa
    28741 attttgattg ggtcgtataa agcgttccat tacccaaatc ttcgtttgtt accataatga
    28801 caaagtttcc attataagtt ccttttatcc caaataaatt gtaattcggt gcctgagcta
    28861 actggctttg accactggca gattctaaga tcgcttgtgc aatcataaca gaagcatata
    28921 aatcatattt ctgaccaatt tttctcgcag actcaccaat tttcataata aaattttcta
    28981 cagattcatt tttgtcaaaa tgaatttctt cattaccaga tagtattggt tgtgattgga
    29041 aagattctgt tgattctatt tgctgtggtg tcgaaaaatt ttttgtttgt ccttccgttt
    29101 ttggcgcttc cttttttgaa gactctatat ggtttggtct tgtcactttc gaagaagtta
    29161 tttcacttgt actacttgaa gttgttgaag attcggcact tgaaatcgta ctactttcac
    29221 ttgacgaagt tgttgaagac tcggcatttg aagtcgtact actttcactt gacgaagttg
    29281 ttgaagactc ggcatttgaa gtcgtactac tttcacttga tgaagttgtt gaagactcag
    29341 catttgaagt cgtactactt tcacttgata aagttgtcaa aagttcacta ctctctgctg
    29401 ttacatcgga attactcatt tcagtcgtct cggttctttc ttttgcaatt ttgtatgttg
    29461 aattttcgct tgccaacaca tggaaaggtg ttgctataaa gagttgggac attaatataa
    29521 ctgctgatgc aagttttaaa gattttttca tttgatttac ctctcttttt tctctttaat
    29581 agtgttttct attaaaaatt atgataaata ttttcaatga attgagtaat acagtatatt
    29641 tcttgatttc atttagtaac gattatgttt caatgtttaa tcctgttact gcctttaaaa
    29701 taaaattata gggttggcta aaactaggat gaaataaata atctaacatt ttcaatttgt
    29761 ctaatgtaat ttgttcttct atagcaagag aaaacattga taatagtcca gataaatctt
    29821 cttgactaca aagttgtaca cctactattc tctttgtatt ttttcaaagc taattcttaa
    29881 ttttacacga ctattttttc tcaaaaaaac aggtctaatg aaatcttcta tttctatata
    29941 atctatagac tcacttaata atcgtgctgt ttttaaattc aaacctgctg cgtaaaaatt
    30001 gtaattaaat atagaaaagc catttgtaaa ttgggaacca gaaggtgtaa aatcttgatt
    30061 gtcaatatgt tgagttgcaa tataagctgt tcttagagca ttagaaatag aaaaatcaac
    30121 actacttcta ttaatggacg taaaaaaagt tgtagcacaa tccccaatag catatatatc
    30181 ttttatattt gtttgctggt attcattagt tagatatgct ccactaacat ctctcaagag
    30241 ttcctttttg ccaattggac tattagatag aaatcctata gcttgtacga ccatatccat
    30301 agagtattgg gtaccacttg tagtgactat tttttcaatc ttttttcttc ctaaatattt
    30361 tttaggactt tctgatagac aaagatgtat tccatttttt gatagtattt cttcaacttt
    30421 atctgaaaaa ctacggtcgt aataagtcga caacactcta tctgcaacat cgaaaagata
    30481 tacttctttc cctctttttc ttatggcttc agcaatttca actccaatat atcctgctcc
    30541 aataattcct acttttttaa tagtattatc atctaaagct ttaaaaatct ctttagcatt
    30601 tggaaaattc tttatcgaaa aaatattagc taaattattc cccggtacat ctaacataat
    30661 ttgctttgaa cctgtagcta gtataagttt gtcatattct tgggaaaatc ttgtgccatc
    30721 tttttttaca caaataatct ttttattttg aatatcaatt ttgtcaactg ctgtctccaa
    30781 aaacaacttt tttatcttag aatttagttc tcttctattt tgataaaaaa gatcttctaa
    30841 attgctaact tctcttttta tgaacaacga tgtcccacat tctaagtaac taatttctga
    30901 ttttttatct atcagaataa cttcataatt tgaatcacat aagtttagaa atcgagctgc
    30961 agctagcccc gaatggtttg atccaataat tactattttt ttcacttaat cacattcttt
    31021 tcccttctat gaaaagtatc ttcattttta actaacaacc tatctcccaa aataaaaaac
    31081 aaaaagagga agaaaaagat tcttcctctt ttcagagaag atttaagaaa ttgattttct
    31141 attcattctc tttttttcga tttttatacc ctaataaccc taaagttcct aacaggatta
    31201 aaccaccact aatagtaaag atattactat caatctctcc cgtcttagga taagcttttg
    31261 aggactgctt ttgaggactg cttttgcctc tatttttgtt ttgcttgcta ccttgattgt
    31321 cagtcttatt atctttacta acggcttttt cactgtcttt ttcgtttgaa gaaacaacta
    31381 cattagtagg ttcactagta ttaccagatt catcagttgc agttactatc acttttgttc
    31441 ctgcctctaa tttaggaact ttaactgtaa aattaccatt tttatcagct gtcccatggt
    31501 attgactccc gtttggtaat gtaactgtca cctctgcatt tggttcagtt ttaccagtaa
    31561 caatttggtc accctctgta ataggatcaa cttgaggttt attcggtgct tttttatctt
    31621 ctacagaaat agtaacctct acttcttctt tagatccatc tggatatgtt actactacaa
    31681 tccctttttt gtttccagct gtactaacat ctactttctc tttccaagta taagtagttc
    31741 cttttggtag atcttctttg ttcttaattc cctcagatgc gtctggctct ttatttaagt
    31801 ctgtagtcac tttttgtcct tcaggattgt atttatcggc atcggaacga ttgtctgtca
    31861 cttctactgg aactttcact gtatctttcg taccatctgg atacgttact tcgataaccc
    31921 cttcgtaatt acctggtgtg ttggtatcaa tcgtaccatc aggagtg
//
LOCUS       DADXTH010000026        31160 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.026, whole genome shotgun sequence.
ACCESSION   DADXTH010000026 DADXTH010000000
VERSION     DADXTH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.026"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(292..3966)
                     /gene="rpoC"
                     /locus_tag="IWI97_001191"
     CDS             complement(292..3966)
                     /gene="rpoC"
                     /locus_tag="IWI97_001191"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBC2462705.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(4024..7641)
                     /gene="rpoB"
                     /locus_tag="IWI97_001192"
     CDS             complement(4024..7641)
                     /gene="rpoB"
                     /locus_tag="IWI97_001192"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBC2462706.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8369..9337
                     /locus_tag="IWI97_001193"
     CDS             8369..9337
                     /locus_tag="IWI97_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2462707.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9355..10260
                     /locus_tag="IWI97_001194"
     CDS             9355..10260
                     /locus_tag="IWI97_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462708.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10257..11063
                     /locus_tag="IWI97_001195"
     CDS             10257..11063
                     /locus_tag="IWI97_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2462709.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11199..12188
                     /locus_tag="IWI97_001196"
     CDS             11199..12188
                     /locus_tag="IWI97_001196"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBC2462710.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12505..13029)
                     /locus_tag="IWI97_001197"
     CDS             complement(12505..13029)
                     /locus_tag="IWI97_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBC2462711.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13316..15070
                     /gene="scm"
                     /locus_tag="IWI97_001198"
     CDS             13316..15070
                     /gene="scm"
                     /locus_tag="IWI97_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBC2462712.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSS
                     TTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVI
                     LLVIGLRITFMSYKKSKR"
     gene            complement(15225..16211)
                     /locus_tag="IWI97_001199"
     CDS             complement(15225..16211)
                     /locus_tag="IWI97_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBC2462713.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16234..16800)
                     /locus_tag="IWI97_001200"
     CDS             complement(16234..16800)
                     /locus_tag="IWI97_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBC2462714.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16814..17230)
                     /locus_tag="IWI97_001201"
     CDS             complement(16814..17230)
                     /locus_tag="IWI97_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBC2462715.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17687..19621)
                     /locus_tag="IWI97_001202"
     CDS             complement(17687..19621)
                     /locus_tag="IWI97_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2462716.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19902..20858
                     /locus_tag="IWI97_001203"
     CDS             19902..20858
                     /locus_tag="IWI97_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBC2462717.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20948..22051
                     /locus_tag="IWI97_001204"
     CDS             20948..22051
                     /locus_tag="IWI97_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBC2462718.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            22064..23017
                     /gene="menA"
                     /locus_tag="IWI97_001205"
     CDS             22064..23017
                     /gene="menA"
                     /locus_tag="IWI97_001205"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBC2462719.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23952..25298)
                     /gene="gor"
                     /locus_tag="IWI97_001206"
     CDS             complement(23952..25298)
                     /gene="gor"
                     /locus_tag="IWI97_001206"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBC2462720.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25432..26685)
                     /locus_tag="IWI97_001207"
     CDS             complement(25432..26685)
                     /locus_tag="IWI97_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBC2462721.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26691..27614)
                     /locus_tag="IWI97_001208"
     CDS             complement(26691..27614)
                     /locus_tag="IWI97_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBC2462722.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27835..30327)
                     /locus_tag="IWI97_001209"
     CDS             complement(27835..30327)
                     /locus_tag="IWI97_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBC2462723.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(30334..30801)
                     /locus_tag="IWI97_001210"
     CDS             complement(30334..30801)
                     /locus_tag="IWI97_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBC2462724.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(30937..31010)
                     /locus_tag="IWI97_001211"
     tRNA            complement(30937..31010)
                     /locus_tag="IWI97_001211"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30974..30976),aa:Arg,seq:acg)
     gene            complement(31068..31141)
                     /locus_tag="IWI97_001212"
     tRNA            complement(31068..31141)
                     /locus_tag="IWI97_001212"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31105..31107),aa:Pro,seq:tgg)
BASE COUNT         9043 a         6616 c         5806 g         9695 t
ORIGIN      
        1 gacatgtcta ctggttttgt tttattcgag gaattattca ataatagcta gcaaaatatt
       61 aaaaataacc gctctatctt cccatcaatt tattttgcat tcaaaaaatg atctatcttt
      121 acttttgttg attgacttct cttggaagat gatcgttccg ataccttaat tggatacgaa
      181 ccgatgttgt taagatcctt tcccactttt cttctagtta gacaaattat tttagacaaa
      241 ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt ttttaaaata tctattttgt
      301 ttgattgttc attgcgtctt ctgcagccat ttgcgcttca atatctgaaa tactgtagac
      361 attttcgctt gctacaccga cttcttttgg ttccatgtca cgataacgtt gcataccagt
      421 accagctggg atgatcttac ctataataac attttctttc aaaccaagaa gaggatcttt
      481 cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt tcttggaatg atgcagcaga
      541 taagaaacta tttgtttcta atgatgcttt tgtgatacct agtaagacag gtcgagaagt
      601 tgcaggaacg cctcctgaga ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc
      661 taataatgta cctggtaaga tatctgtatc acctggatcc attacacgaa ctttacgcag
      721 catttgacga accattactt cgatatgctt gtcgccgatt tctacccctt gcatacggta
      781 aacacgttgt acttcacgaa gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa
      841 caattgtttt ggatcgattg atccttctgt caatggtgca ccacggtgga tcatatcgcc
      901 ttctgctact ttcatacgtg ctgtataagg aacagtatac gtacgtgtat ctgtttttcc
      961 tttgattgtt acttctttgg tacgtgttgc aggatcttct gagatatcga tgacatcccc
     1021 tgttacttct gtgatcactg cttgcccttt cggattacgt gcttcaaaga tttcttggac
     1081 acgaggaaga ccttgagtga tatcgtctcc ggcaacccca cctgtatgga atgtacgcat
     1141 cgtcaactga gtaccaggtt caccgattga ttgagcggcg atcgtaccaa ctgcttctcc
     1201 gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg
     1261 ttttgtatta catgtgaaga cagaacggat tgtcactgtt tcgacacctg catcaacgat
     1321 ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca atgatcaatt catttgtttc
     1381 aggatgacgt acttctttac gggtgtaacg tcccactaaa cgatcttcta atgattcgat
     1441 gatttcattt ccctcgcgga tggcttggat ttcaagacct cggtcagtac cgcaatcttc
     1501 ttcacggatg attacatctt gggccacgtc aaccagacga cgagtcaagt aacctgaatc
     1561 ggcagtcttc aaggctgtat cggtcatccc tttacgagcc ccgtgagttg agataaacat
     1621 ttccaagacg gatagtcctt cacggaagtt cgaaatgatt ggcaattcca tgattcgacc
     1681 attaggagcg gccatcaaac cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc
     1741 acgagctcca gaatcagaca tcatgaagat tggatttttc gcttccaaac cttccatcag
     1801 tttttgttgg atgctgtctt ttgcagcatt ccaaacagca atgacacgtt cataacgttc
     1861 gtcatctgtg atcagaccac gacggaattg ttttgtaata gtttcaactt gtttatgggc
     1921 attttcaatg atttcttgtt tttcattcag tacactgatg tcagcgatcc ctactgtgat
     1981 accggcatgt gtcgaatgtt tgtaccctaa gtctttcata cggtcaagca tcttagaagt
     2041 ttcttttact ttgaatcgtt tgaagacttc tgcgatgatg tttcctaagt ttttcttctt
     2101 gaacggtaat actaattctt gctctttgat atgtgcagga atatcagtac cagcttcaac
     2161 aaagtatttg tccggtgttt ggacggtcaa gttgaagtct gttggttcat tcagataagg
     2221 gaattcagga ggcatgatct cgttgaagat gatcttacca actgttgtga tcatcgtacg
     2281 ttctttttgc cattcagtaa atggtttttc acctaatgag ttagggttca caccgatacg
     2341 tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa
     2401 gaccattcct tcgccttcgc gtccttcttc ttccattgtc aagtagtagt tacctagaac
     2461 catatcttga gaaggagtaa caactggttt accatcttta gggttcaaga tgttttgagc
     2521 agctaacatc aacatacgtg cttctgcttg tgcttcttca ttcaatggaa cgtggacagc
     2581 catttggtct ccatcgaaat cggcattata agcttcacat acaagtggat gcaaacggat
     2641 tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg
     2701 tgcccggttc agcaatactg ggtgttcctt gattacgtct tctaaaacat cccatacttc
     2761 gtcttcttga cgttcgattt tacgtttcgc atttttgatg tttgaagcta gttcgcgttg
     2821 aacaagctca cgcatcacga atggtttgaa taattcaatg gccatttctt ttggaagtcc
     2881 acattggtac atcttcaaga aaggtccaac aacgataacg gaacgacctg agtagtcgac
     2941 acgtttacct agcaagtttt gacggaaacg accttgtttc cctttcaaca tatgagaaag
     3001 agatttcaat gggcggttac ctggtccagt aactggacgt ccgcgacgac cgttatcgat
     3061 caatgcatct actgcttctt gcagcatacg tttttcattt tgaacgatga ttccaggtgc
     3121 attcaagtct aacaaacgtt tcaaacggtt attacggttg attacacgac ggtaaagatc
     3181 attcaagtca cttgttgcaa aacgtccacc ttctaattga accattgggc gtaaatctgg
     3241 tggaataaca gggataacgt ccataaccat ccagcttggt tgatttcctg aagcgcggaa
     3301 tgcttctaaa atatccaaac ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt
     3361 caattcttct ttcaattcgg ctgcttctgc atctagatca acattatcaa gtaattgttt
     3421 aatcgcttct gcacccatag cagcttggaa ttcttgtcca tattgttcac gtttttcgcg
     3481 atattcgcgt tctgtcaaca attgtttttt ctctaaattc gtatttcctg gttcgatgac
     3541 tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt gggctcatgt ctaatacaag
     3601 acccatacgt gaaggaatcc ctttaaagta ccagatatgt gaaacaggag ctgctaattc
     3661 aatatgtccc atgcgttcac ggcgaacttt cgaacgagta acttccactc cgcatcgatc
     3721 acaaacgatt cctttataac ggatacgttt gtattttccg caggcacatt cccagtcttt
     3781 tgtaggacca aaaatccgct cacagaacaa cccttcgcgt tcaggtttta aggtacgata
     3841 gttaatcgtc tcaggttttt ttacttcacc gtaagaccag cttctgattt tttcgggaga
     3901 agccaaacct atttgcatac tttcgaattt atttacatcg atcaaaaggg gttccctcca
     3961 tttcatttaa tggcaaaccg caagacggct gatcttcgac cgccttgcag ctcatagacc
     4021 ggattagtct aaattatctt ctgcggtttc aaagttttgg atgacatcgt ctttttcatc
     4081 ttccacttgt tcagcagctt tcttttctaa ttctttggca gtttgctgtt cggcaaattt
     4141 tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat
     4201 ctctgtttct tcgatatcca agacacgcat gtccagacct agtgattgca attctttcac
     4261 caatacgcgg aatgattccg gtacacctgg ttttggaatt ggttcaccct tgacgattgc
     4321 ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg tatgtcaaga tttcttgtaa
     4381 agtataagcc gcaccgtaag cttccagtgc ccatacttcc atttcaccaa aacgttgtcc
     4441 accaaattga gctttacctc ccaatggttg ttgtgtaacc aatgagtaag gtccgattga
     4501 acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg atcatgtaca tcacaccgac
     4561 agacacacgt ccatcaaatg gttcacctgt acgtccgtcg tacagaacag ttttcgcatc
     4621 gctggccata ccggcttcac gaactgtttc ccatacgtct tcatcacttg caccatcaaa
     4681 tactggcgtt gctacgtgga tacctaactg acgtgcagcc atacccaagt gtaattccaa
     4741 tacttgcccg atgttcatac gtgaaggcac ccctaaagga ttcagcatga tatcgattgg
     4801 tgttccatca ggtaggaatg gcatatcttc ttccggcata atacgggata caaccccttt
     4861 atttccgtga cgaccggcca ttttatcccc ttcatggatt ttacgttttt gaacgatata
     4921 aacacgaact aacatgttta cgcctggtga caattcatct ccagcttcac gagtaaagat
     4981 tttcacatcg tgaacgatac cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac
     5041 ttcacgagct ttttcgccaa agatcgcatg caatagacgt tcttctgcag ataattctgt
     5101 tacccctttt ggcgtgactt tccctacaag caaatcgcca tcttggactt ctgcaccaat
     5161 acggatgata cccatttcgt ctaaatcttt taatgcgtct tccccgacat tagggatttc
     5221 gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct tctgattcat attcttcaat
     5281 atggatagaa gtgtatacgt catcttttac caaacgacgg ctcatgatga tcgcatcttc
     5341 atagttgtac ccttcccaag tcatgaaacc aactaaaacg ttttggccta gagccatttc
     5401 gccttgttcc atagatggtc cgtctgccag agtatcccct ttttcgactt tctcacccaa
     5461 atgaacgatt ggacgttggt tgtagcttgt tccggagttt gaacgacgga attttgttac
     5521 atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta
     5581 ttcaacgaca ccatcatgtt tacataacaa agcagctcct gagtcatgcg ctgatttgta
     5641 ttccatacca gtaccaaccc atggtgcttg aggattgatc aacggcactg cttgacgctg
     5701 catgttggca cccatcaagg cacggttgga gtcatcgttt tccaagaaag gaatacatgc
     5761 tgtcgcaacg gcaactactt gtttaggtga tacgtccatg tagtctactt tatcaataga
     5821 tacttctaag ttttcacttt gagcacgagc cataacaacg tcttgagcaa atgttccatc
     5881 ttcattcaat gggctgtttg cttgggctac gatgtaatgg tcttcgatat cagctgtcaa
     5941 gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct acacgacgat aaggtgtttc
     6001 gataaatccg aatttattta ctttcgcata actggacaaa ctattgatca acccgatgtt
     6061 tgggccttca ggagtttcga tcggacacat acggccatag tgggagtagt gaacgtcacg
     6121 aacttcataa ccggcgcggt cacgagtcaa tccgccaggt cctaaggcga aaagacgacg
     6181 tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg aactgtgaca actgagaaga
     6241 accaaagaat tctttgatac ttgccacaac tggacggata ttgatcaatt gttgaggtgt
     6301 caatgtttca gtatcttgga tcgacattct ttcacgaacc acacgttcca tacgtgctag
     6361 cccgatacgg aattggtttt gtaaaagttc accgactgaa cggatacgac gatttcctaa
     6421 gtggtcgata tcatcgacat tgccgatacc ttccatcaag ttcaagaaat agctcattga
     6481 agcaatgatg tcggctggac gaaccgtttt tacagctgct tctggatagc cgtttccgat
     6541 cacgtttact tcacgttcag ggtctcttgg tgaaaatact ttgatgactt ggacagtcat
     6601 tggatctgtg accacaccgt cttctgatgg atagtaagtc actgaattta ggccattatc
     6661 aatgaaagga gctagtgttt ccatgacttg gtgagtcaga actgttcctt tttcaacgat
     6721 gatctcgcct gtttctggat caactaatgt ttcagctaat gtcagattca atagacgtgt
     6781 tttcaaatct aattttttgt ttactttgta acgaccaacg tttgctaagt cgtaacgttt
     6841 tggatcgaag aaacgagcat tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc
     6901 tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct gttcttgagt cgcttgcgtt
     6961 tttatgcaag tctttttcga ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa
     7021 gatcgtatca tcggaaccaa atcctaacgc acgaactaat acggttaaag gaatcttacg
     7081 tgtacggtcg atacgtacat aagagatgtc ttttgcatca gtttccattt ctagccaagc
     7141 tccacggtta gggataacag tagaaccgaa tccttctttt ccgtttttgt cgacttttcc
     7201 atggaaatag acacctggtg aacggactaa ctgagaaacg attactcgct ctgccccgtt
     7261 gatgatgaat gttccttgtt ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc
     7321 tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta
     7381 gtttgcatca tgcgcacgag cttctgctac tgtatacttt ggttctttca attcataatc
     7441 tacaaattcc aaagatagat tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat
     7501 ttctctaagt ccctcatcta agaaccattg gtaagagtct gtttgaattt cgatcaaatt
     7561 tggtaattcc aacacttcac tgattcttgc gaaacttcta cgttcgcgat gttttccgta
     7621 ttttactacg tgtccagcca agctcttcac ccctctaaaa tgtcattcaa aaagactacc
     7681 cgatcaattg ttacaataat atttcaaata ttatttttag gtaacaacag acattttttg
     7741 cggcttttag gcaaaaaaaa accaaaaata gaacattctc ttgaaacctt acttcgtgag
     7801 cttctacttt tgtttcctta tattaaagcc gaaacggaca atatttcgtt tctgcctttt
     7861 cttcatcaga tagtttttgc aacttcatat actacactta ttttctataa aagtcaacac
     7921 ttcctggcgt ttcttctgct ttttcgcaac attttcttaa tgttttcaat tttatgtttt
     7981 caattatctg acaattctta attgacagac ctttttatct cggttatact ttagaaaatt
     8041 cattttgtgg aggaacttct atgcgaacca acatcaacca aatcgcacca acacaactga
     8101 ataactttta ctttagctac tttagatagg tttgtattca tcatgatgga tacaaacgtt
     8161 aaacgacagt ttgtatctat gatggctaaa gaactttgta ttgcagcaaa aagccacgta
     8221 gacaaaatgc taagtggctg agcagtgaaa atacaaagta aaagtatcaa gcattctact
     8281 ttttcaattt attttccgac ttttcaatcc atttggacat tttgtctgaa tggatttttt
     8341 gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc
     8401 tgccctgcta ctagctttag gaggttgcgc acaaaaagac aattccgcag aggcatcaga
     8461 tggacaaaaa acaattggcg tattgcaact tgtacaacat aactcgttag atgctgccta
     8521 taaaggattc aaagaaggat tagcagaaga agggtataaa gaaggcgata atctaacaat
     8581 cgattatcaa aatgcccaaa acaatcaaga caacctgaaa agtatgagtg agaaactcgt
     8641 caaagaagat cctgatttgc tacttggaat cgccactcca gcagcacaat ctttattaaa
     8701 tgaaacaacc actatcccga tgactgtcac agcggtcaca gacttggaac aagcccattt
     8761 agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc acgacagata tggtgccaat
     8821 cgaaaaacaa atcggcttac tgttaaatat cgtgccagac gccaaaacaa tcggcatcat
     8881 gtataacaac ggtgaagcaa actctaagat ccaagctgat ttggcagaaa aagaattgaa
     8941 aaaagcgggc gtcaaagtca aagtactgac agcaaattcc acaaatgatg tccaacaagt
     9001 gacgaaaagt ctagctaaag atgtagatgg aatctatatt cctactgata acacgttcgc
     9061 aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg
     9121 cgcagttgat caagtcaaag acggcggtct agcaacagta ggaatcgact acgaaagctt
     9181 gggcaaacaa acagggatca tggcagcaaa aatcttagac ggcaagaatc caagtgaact
     9241 tcctgtcgaa acagcgaatg atttggaact gtatgtcaat aaagacatgg ccaaagcatt
     9301 aggcatcaat ccagacagca tcaaagaagc tgaataaaac acaggaggat tctcatgaca
     9361 aatttgctct ctcttttctt atcatctgca tcgcaagggg tcttatgggc cctacttgcg
     9421 attggtgtat acgtcacttt ccgtatcttg gacattgccg acttgactgc tgaaggaagc
     9481 tttcctttag gtgcagggat cgcggcagta agtattacaa atggctatca tccattgaca
     9541 gcttgtcttc taggcttttt agggagtgcg gcagcagggc ttgtttccgg actgcttcac
     9601 acaaaattga aaattcccgc ccttcttgca gggatcatca ccatgactgg tttatattct
     9661 gtcacatccc gtgtgatggg tgcgccgaac atctctttat tgggtcagcc aacgatcttt
     9721 cattgggtcg agtcattcgg cataacaaaa gacaacgctg tactgatcgt cggtctgctt
     9781 tttgctttga tcgttgtatg tttacttgtt ttgttcttac gtacagaaac aggacttgct
     9841 attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa acgggatcaa cacagataat
     9901 atgaagatca tcggttatat gatttctaat ggatgcattg ctctttctgg ctccttgctt
     9961 gcgcaaaaca acggctttgc tgatttaaat tctggtattg gaacgatcgt aattggactt
    10021 gcttctatta tcatcgcaga agttttgtta cgtaaccagc cactatttct ccgtctcttg
    10081 actgtcgttt taggtgcggt catttatcgt tttattttag cgttagtctt cgaactgaat
    10141 gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc tagtgatctg tctttctttc
    10201 ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga aaggcggtag tgtcaaatga
    10261 atgacgtctt aacattggaa aatctccatc atacctttga aaaaggaacg atcaacgaaa
    10321 atcatgtcct gaaaggcatt aatctttcct tagatcccgg cgacttcgtg acgatcatcg
    10381 gtggaaatgg ggctggtaaa tctactttgc tgaacagcat cgccggtaca ttttctgtcg
    10441 atcagggtca aattttattg aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt
    10501 cgaaatccat cagtcgcgta ttccaagatc ctaaactagg gacagccgtt cgtttaacgg
    10561 tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg
    10621 tcaaaccaca agaccgaaca tttttcaaag accagctggt tcggctaaat ttggggttgg
    10681 aaaatcgttt atcctcagaa atcggtttgc tgtctggagg acaacgtcaa gctattacgt
    10741 tattgatggc cacattacag cgtccagaac tgatcttatt agatgaacac actgcagcgc
    10801 ttgaccctgc tacttccaaa acggtgatgg aattgacaga aaagctgatc actgagcaga
    10861 atctcactgc ttttatggtc actcatgaca tggaagacgc gatccgctat gggagtcgac
    10921 tgatcatgct gcatcaagga aggatcgtcg tagatatcca gcaagaggaa aaacagcagc
    10981 tcacagtgcc ccaattatta gcgttgttca aacaaaacag cggcacggca ttaaaagacg
    11041 atcaagtact tttatcttca taatcagaaa gaggatggga caaaaaatga acgcccaccc
    11101 tcttcttttt tctctttcct tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg
    11161 aaaactattt tgttctctcc ttcttcctga tagaatggat gggaaaggag cttgatgcca
    11221 tgacaacgaa agacaaggta cttcaattat tgaaaaagac accagatttt ctttccggag
    11281 aaaagttggc acagcaactg gaagtttccc ggacaagtat ttggaaagcc atcaaagaat
    11341 tggaaaaggc aggctatcgt tttgagcacg ggcctacagg gtatcgttac ctgccctctg
    11401 acgtactaga tgcaaaagaa atccagcaag agctacggtc gtttattcct gatttgaaag
    11461 tacaaacgat gatgacttct gaatctacga tgaaagatgc caaactggca gcagctcaag
    11521 gagattctgt ttcgaagtta tttgttgctg atacacaaga acaagctcgt ggacgttttg
    11581 gacggccttt tttcgctgaa cctggacgag gaatctacat gagtctcttg ctccatccag
    11641 acaaacggtt tgatgaattg cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag
    11701 ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg ggtcaatgat atctacatgg
    11761 atggaaaaaa agtctgtggg attttatctg aagcagtcag tgatatggaa agcggcagga
    11821 tcagtcatat catcattggg atcgggctta atttttctgt tcctcaaaat cattttccag
    11881 ctgatctcca agaaaaagcc atctctgtct ttcccgacgg acaagctagt gtcacaagga
    11941 atcagctaat cagcaaaatc tggcaagaat tgttcactta tctttctcac ccagaaaaag
    12001 cgttggactt ctataaagaa aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa
    12061 aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc
    12121 aaacaaatga acagaagatg agtttgtctt ctggcgaaat cagtctcaca tctatccgta
    12181 cgcaataatt atcagatttc acaaaaggac acctgatctc atacagtgtc cttttttctg
    12241 aattctcttc aaaagactct ccttattcta tcagctgaca gtttctctat cctctagaaa
    12301 aaggtattct aaaaatatct ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa
    12361 aaacaagaga gtcatcagtt tacacgatca aaagagatag caggccgctt cctttttcaa
    12421 tcttatagat agaccatggg taacgataaa aacaaaaaag aacttgcgac ttattttgcc
    12481 gtaagttctt ttttagctag tgccttaatc tcttaatccc aacaaacgtc tcgcatagat
    12541 cacctgacca gagtgcatag cagcatcatc aataatagaa acgagacgca ttcctcgtgt
    12601 gactggtggt gtccagttct cgtcaattac ttcatctagc tcggaaggtt ccagccttct
    12661 taagtaagaa atagcaaaat cagcagctcc tcgcaagtat cctagcagat cttctatttg
    12721 attagcttgg atctgctttg cttgttccag actatgcttc caatcttgtt cgttatctgc
    12781 tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc caaattggag aagttcctgc
    12841 taaatcagca atctggaaat ctaattctcg agcggtatgc caagccagcc aagtcattga
    12901 tttgatctgc ggggctctat cttccactgg aaaacgattg gcctcctcaa tagagacgcc
    12961 ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca ctctcgcata gcatatctaa
    13021 ataatccatt tattttcctc ctttttattt atcctactta ctttttccta attctaggat
    13081 acctacttat atgttactac acacccttat tttttacact ttaacagtca ccatcatcaa
    13141 tagtttggag attgaaagaa aaagatagtg tcgaactatc aattgaacaa gaagcaacaa
    13201 tatgaacgtt tcattaatat tctttaaata aacaatgatt tttcaaacga acagtttaag
    13261 actaggagaa aaagagtata ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa
    13321 aaaaagggca agtttactag tcctagttgc tttgttgttc aatatctttt cgtatggtgt
    13381 ggccgtttca gcggaaacat acattcctaa agaccctggg aatatttata atgatctaac
    13441 tggtaactat ggcttgttag gaattgctag tcaattccac gtatttgcta aaaataagac
    13501 aactgtgaat gcacatacag acggaaatat agcaacacat gaacttgatg caaaaaataa
    13561 ttttggtaca gagcttcatg aaggtttatt gaaccaagaa ataaattata tacaaaaaac
    13621 tgattctttc gctaattctt caggcatacc tacaggtagc acaagaatga ataaatttgt
    13681 cgttggcagt aacgtggatg tagcacttca agatggtcat cctacagtca atggatccag
    13741 gcttgatcac ttaactccag atgattttta taaagatcgt gctgggcatg aatatattga
    13801 tttcgagcag gaatttgcta aattgaatgt agcagcaaat gatcttgcaa cgatcactcc
    13861 agctaaaaca tatactgctg cagattttcc agacatgaac aatcggacaa tcgatttaac
    13921 tgatttatca gataccgggt ttttattggt aaatatcgat gcagaagtgt taacgatgaa
    13981 tacgccactt caaatcatta atccaaatga tcaagtagtt gtatttaatg tcatcaacag
    14041 ttcttctgca ttgaacgtac agtcaccaat caaatacaat gatcgttcaa atcatgaaac
    14101 agaagatttt tcggatgcga atatctcttg gaattttgga aatgagatga ctgatttgac
    14161 aatcagtgct ccttttcaag gaacaatcct tgcgcctaat gctactatcc gcattactca
    14221 aaaccaagac ggaacgatta taggaacaaa tgtgatttta gatgcagcta ccaatcgttg
    14281 ggatcctaac gaaatattta tcagcgacaa tggtacagat acgacagaca caagcgacag
    14341 tacagataca tctaccacaa gtgatagctc agattcatca acttcaagcg acagtacaga
    14401 tactacctct accacaagtg atagcacgga tacatcagct tcaagcgaca gtacagatac
    14461 tacctctacc acaagtgata gcacggacac atcagcttca agtgacagta cagatactac
    14521 ctctaccaca agtgacagca cggacacgtc agcttcaagc gacagtacag atactacctc
    14581 taccacaagt gatagcacgg acacatcagc ttcaagtgac agtacagata ctacctctac
    14641 cacaagtgat agcacggaca cgtcagcttc aagtgacagt acagatacta cctctaccac
    14701 aagtgatagc tcagattcat ctaccactac aagtgatagc acggacacgt cagcttcaag
    14761 tgacagtaca gatacatcct ctaccacaag tgatagctca gattcatcaa cttcaagcga
    14821 tagtacagac acgtcgtcta ccacaagcga tacacgtcat gcaagtgtct ccttctcaaa
    14881 aacaagttcg gataacagga cgaactttaa cttaactaat aaaacaagca atgacaatgg
    14941 aaacaaatca aaaagagcac ttcctaaaac tggaagtcaa tcgaataatt ggataacact
    15001 cgcaggcgtt atattacttg taatcggttt aagaatcact tttatgagtt ataagaaatc
    15061 gaaaaggtaa aaaaatattc aagataaaaa agagcaacgt cgctaacgga cgttgctctt
    15121 tatttttgct ttttatcata aatacaagaa ctacaattca atttgtcaaa aaacacgtag
    15181 atttccttct gagaaattta tttacgtatc gaacctttcc ctattcagtc tttgcgatcc
    15241 aaaatcagtt gggtaaggga aaagagattc tcttttgttc gaagagaggt ggcaggtaat
    15301 ttctttattt ccttcaaagc cttttctgtg taagaggccg ctaacttcct agcttcttcc
    15361 acgcctcctg tatgttcaac gattttgcgt acggcgtcga catcttcttc tgtcattgcc
    15421 gctttcttct ctaaataagg tagtagcttt ggtttgtttt tttgcaaaga gtagatcaac
    15481 ggtaaagaat agaccccttg acgcatgtct tctagtacag gtttgccgat ttcctggctc
    15541 tcttttgtat aatctaaaat atcatcgata atctgaaagg ccatcccgat agaaagtccg
    15601 atatctcctg ctttttttgc gaagcgctcg cttgttccac tttcaaaagc gccaacagaa
    15661 caacttaatt ggaacagttc agctgtctta cctgaaatat tttccaaata ttcttttaca
    15721 gaaagttcat aattatagcg atcatccatc tgccctaatt cgccattcaa tactttttcc
    15781 atactgcgag aattcaattg gagacttttc aaggaagtgg agtaatcaga caatagttta
    15841 aaacagcaga caaataaata gtctcctgca tagacagaaa cgacattacc aaactgacta
    15901 cggatattag gctgaccacg tcgcagatcc gcttcatcaa cgatatcatc atggatcagt
    15961 gtggccgtat ggagcatttc gatagaagct gccaaagcga tggctttgtc tcgatcttgc
    16021 tctggtccaa attgtgagaa caatagctga taggcaggac gcaacagctt tccgccggca
    16081 tggatcgttt ctttgattgc ttgttcgaca ggtttgtttt tcaggcggac agtttcttcc
    16141 atccgtttta gtgtttctgt caattcttta cttaaagcag gatagttttc ccacattgga
    16201 tgtagcttca taaaaaaact ccttgtgtga cgattatttt ttggtaaaca ttgggctcca
    16261 tttttgtttg gttccctgct cataatcttt ttggaatcgt tgaagtgtct gcacatgtcg
    16321 taaaagataa ttcgctgcga tcccgattgc aatccctgat aaaatcccta aaactgaaag
    16381 gactggcaag tacaacatga ctgtccatga ttgtgcaatg aaagaagcca tcaaaagctg
    16441 tccgacatta tgcatgaaac cgccggccgc actaattccg atgatactga cacgttttgg
    16501 tcctaattgc ttgaccaata acattccaaa ataactaagc aaagcaccac tcatactata
    16561 aaggaatgtg gaaatcgttc cgcctagcaa agtagtcagg aatagtcgta gccaaagtag
    16621 taagaagctg tcttgttttt tcatggtaaa aatggcaacg atcgtgatta gattggctaa
    16681 gcctagtttt gcaccaggcg caaaagaaaa aggataaggg atcatatttt ccaataaacc
    16741 aatgatcact ccttgtgcaa caagtagcga aatataaatc attttttgta atttactcat
    16801 ggtgtgtgaa ggtctaataa atcagactgt cttcgtcacc tccatctgtt gaacggactt
    16861 cgatcactaa tttatgtggc aaacagacga tggtttcgcc atttttcgtt gcccagcctc
    16921 gacgtacgca gatctggtca tcgcaatctg cttcttttat ccgtactcga tcgccatcta
    16981 tctcgatcaa gttataatct ccgtgttcat ctttgtaagt atatgtgttg ctctcttctc
    17041 cagccatcaa agggaatgtt ttgatttctg tgccatccac acgcagtacc gcttcttttt
    17101 ctgctgttgc ggtgttttgc tgaagaccaa aaatcatgac tggtaaaaaa gaaagaacag
    17161 tcaaaagcaa aataataaaa acatcccaag gtttgagcag acttttttca gcaaattctt
    17221 taaggctcat ttttgttctc ctttttccag attaactcac ccaattttag cataaaaaca
    17281 agaggttgtg acacctgtgt cttgctttga gtaataagaa cgaagaatag aaaacagctt
    17341 tccatgtttt ctaattcttt gggcttatta ccgagaagca gcaggtggaa caccgtttat
    17401 aacaagaggt tgtgacagaa gtgggcaact tcaagaaata agaaggaatt tacgaaaatt
    17461 acttttcaag tttttgtgaa atttcggctt atttttcgaa gaagctactt ctggaacacc
    17521 gtttataaca agaggttgta acagaggttg tgacacctgt gtcttgctca tggaggctta
    17581 tctcgggcat cttattcctt taattttaca taaaaaggag gctgtgacgg tttgtctcag
    17641 cccctttcta ttgaaatctt gcattcaaat aacgttcttt atctatttat ttcttgccgt
    17701 actgggaact tggtctaccg taccaccaat tacctagtgt acgttgtagc catggaatta
    17761 cccagcgatc caacccaatc gcacgtccgg aaccgttcat caatgcgaaa gcaacaaaaa
    17821 tgaaccaaat gtttacccag taaaacatgc ctgataagca gaacgtgatc accaatacaa
    17881 tcgttgtcgc actgcatagc catgtgaata atccagcaat caatgctaat gccaaggcaa
    17941 tttcaaagaa taccatgaat ttttggaaga acattgctac atcattgttt ggcatgatca
    18001 cttgcatgat ctttgtgaac cagtctggtg acttctcaat cacagccatt ggttcttcac
    18061 cgtatgcata actcaaacca aaatgtgcag cttgagtcgc tgcttctccg gcacttgccc
    18121 ctgctccgct ggcagctgtg gttgcatcac cagcagcaga agcaccactg gcaacatctg
    18181 ttacattgta ttcccatgtc caagggaaca cactgtttcc agtgaaccat gatccttcac
    18241 caaaccaagt acttggtttg aacacttcac cattcccaat gatttttttg gaagcttcaa
    18301 cgaaccaaac taaaccatag aaaatacgaa gtggcacact ccataagaca ttgccgtaac
    18361 gagaagaatg tcctcgagtc acgttgcgat catctttgat atggaaaaac tcatgcatta
    18421 aatattggaa catataataa ccagaacgaa tatcaaagaa gtatttcaaa ttcacgatat
    18481 gtttcattac gatagctaaa aatccgctta aatggatctt atcaaatagg ttagctaccc
    18541 cccatttggc accaatagaa accataaatc cttgatagtt tcctttaaat ttatgctttt
    18601 ctccgccttt gatcgaagcc acgatattcg ctgcggctgt gtgtcctgtt tgttcagcag
    18661 cttgaacgat ctgcggtgtc ggtgttcctg ggaattcttc ataaaagact aaatcaccaa
    18721 tgatatagat gtttttatct tcgtagcctt ttgcctgcat atattcattg gcgatcaacc
    18781 gctgtccgcg tgctgattct aatccaaagt cagcagcgtc agaagttcct ttgacaccag
    18841 ctgtccaaat cagtgtgtga gtcggaatgt tgcgtccgtc tttcaatttg atatgatcag
    18901 cagccacttc tacgattggt gcattcaata ataattccac gttgtgctta cgcaaatagc
    18961 gttcagcttt tgcagcatca tttcgatcca acatattcaa gatcgtcggc attgcctcta
    19021 cgaccatcag tgtgatttcg tttggatcta atttataatc tttggctagg cgatttttcc
    19081 agtcgatcag ttccccaatc atttcaatac cggtaaatcc agaaccacag acaacgaaag
    19141 tcagcatggc tttacgcagt tcatcatctg gttccaatgc agcttttgct actgtcttct
    19201 caatatgttc ccgaatcctt acagaatctt caaatgacca aagggtgaat ccgtgtgctt
    19261 tgacaccagg tgtaccaaag tcattcggct ctccgcccat tccaaggatc agctgatcaa
    19321 aaggatagga acctaatttg gtcttgacga tttttcgttc tttatcgata ccgattacag
    19381 tgtctgtgac aagttgtaca ttcttttgac gagcaaacaa atgctgcaga tcgtattgga
    19441 ttgcttctgg ttctacacgt ccgccagcta cttcatgcaa ctcggtcatc atagtatggt
    19501 acgaatgacg atcgatcaga gtaattgcta catcttcttt tttcagtttc tttgccaagt
    19561 attttgtagc tgaaacaccg gcatatcctg ctcctacaac gacaatattt tgctttgcca
    19621 ttgatcatcc gctccttata gactatctat ataatgaata tatgataatt cttttagttt
    19681 gatacttatt acacaatctc catatattat atagatgttc tgttcttttg tctaactcct
    19741 tttggatttt tttcaaattt taaaataaag cgttttttca cgaaatttgc tgaaagaaaa
    19801 cgtttgaaat attacatggg ttatttatca aaaatagttg acttataggc taaaaagatt
    19861 agaatgtata atgaatcaca aagaaaaatt gtggactaga aatggaatgg tggggtatta
    19921 aaatgaagaa aaaacgtttt gtaactggat ttgccgtatt agcattttca gctctagtgt
    19981 taggcgcttg cggcgcagat gacaacggaa gctcaaactc ttcttctgaa tcatctacag
    20041 cacaatcttc gactgcaaaa agcacaacag aatcatctgc aaaagttgta gctggcggag
    20101 atttgcaaga cggcacatat aaattagaag aaaaaaatta ttcaaatggt taccgtgcag
    20161 tctttgaaat ggttgtaaaa gacggcaaaa tcactgaatc aaaatatgat aacgtcaatg
    20221 aaaacggcga atcaaaaaca aaagatgcag aatacaacaa aaacatggaa gcaaaatctg
    20281 gcacaaaccc agaaaaatac attcctgagt tgaatgacca attcttaaaa gcacaaagcg
    20341 caagcggtgt ggaagtcgta actggagcta ctcactcatc tgaaagcttc caaaactacg
    20401 cacaacaatt gatccaagct gctcaagctg gtaacacaga tacaatcgaa atcgacaatg
    20461 gtgctgattt gaaagacggc acttacaagc ttgaagaaaa gaactactct aacggctacc
    20521 gtgtgcaatt cgaaatgaca gttgctggcg gcaaagtaac tgaatcaaac tttgattata
    20581 tcgacaaaga cggtaaatca aaacaagatg atacagaata taatgaaaat atgaaagcaa
    20641 aaagcggtac agaaccaaaa acttacatcc ctactctgaa cgacgaattc gtcaaagcaa
    20701 tgggcgaaga agatggctca cctgctaatg tagaagttgt tacaggtgct acacacagct
    20761 cacattcatt catcatgtac gcacaacaat tagtgaatgc agctgaaaaa ggcgacactc
    20821 aaacaatcga agtagataat atcgtaacag aaaaataatc acataatcat actaataaaa
    20881 gttggagggt gggacgatag ccagattagg cagtcgtctc acctttcaaa atgaagagag
    20941 gaaaaagatg cggaaaaaac tactattttc cttaatcaca ctgggcatct cttttatggt
    21001 gctaactggc tgcagcaact cagcaaataa aacaaccact gaaagttcag cagctaccaa
    21061 gatattgaaa gatccatatt ctgatgaaca gttcttgtta gggacttacg ttcgtatccg
    21121 tgtgtatgat gaaggcaaag aatcagcatt gaaacctgcc ttcgatcgag taaaagaact
    21181 tggtgataaa atcacgatca atcaaaaagg ttctgagatc gatgaagtga atgagcaagc
    21241 tgggatcaaa ccagtaaaag tttctgatga cgtctacacg ttagtcaagc gggcctacga
    21301 atatagccaa gattctcaag gcggattcga tatggcgatt ggtgcaatca cacaattatg
    21361 gcgaatcggt tttgatgacg ccagaaaacc ttcccaagaa gaaatcgatc aggcattgaa
    21421 attagtggat tatcataaga tcgaactaaa tgacaaagaa aaaactgttt atctaaaaga
    21481 aaaaggaatg atcatcgatt taggtgcaat cgccaaaggt tacattacgg acgaagtagt
    21541 gaaagtcttg aaaaaacaag gagtgaccac tgccatcgtt gacttgggcg gtaatgtcta
    21601 tgtcttaggc cacagcccaa gaggcgaaaa ccaagactgg acagtcggca tccaagatcc
    21661 gaacatggct cgcggatctg ttttaggttc tattaaagaa cggaacaaaa cattagttac
    21721 ctctggaatt tacgagcgtt atttagaagt agatggcaaa aaataccatc atttgtttga
    21781 tccaaaaacg ggttatccgt tcgacaatga tatcgcaagt gtcacgatca taacagataa
    21841 atcaattgat ggtgacgggt tgtctacagc agtcttttca atgggtgtga aaaaaggact
    21901 ggaatacgtc gaaagcgaat tgaataacgg gacggaagcg atcttcgtga caaaagacga
    21961 taaagtatat gtgaccgatc caatcaaaga caccttcaaa ttatcggagg actcccatta
    22021 cacgatgggt gaccgttctg aactaaaata aggagtacga accatgacac taaaaatctt
    22081 tttagaagta gttgaaatac gaacaaaggt ggctagtgta tttccttttg cggtaggggt
    22141 attattctct atcgcttttt ttcatgagat acattggcta aacacttttt tattttttat
    22201 tggaatgcta gtttttgatt tagcgactac cgctatcaat aattacatgg attatgaaaa
    22261 ggctcattct gaagtatata agtatgaaga aaatgtcatt ggccgcagaa atctatctcc
    22321 tgtattgatt cgaaacatga ttttagctat gattgcattt gtttttgtta tcggcgttat
    22381 tctaacgatc aatacgggtt ggcttttact tttgatggga tttatctgtt gttttattgg
    22441 agtattctat acattcggac cgattccttt atctcgaatg cctttagggg aaattttcag
    22501 tggggtgact atgggccttg gaatttttgc catggtcgtt tatgtcaata cattcggttc
    22561 agatgtctat gatctgacag tcaacttttc atctggacaa tttgctttgg taggaaatct
    22621 ttgggctata gcagcaatcg tattagcttc cttacccttg gtgtttacga ttgccaatat
    22681 catgttggct aataatttgc gggatcttga ccgtgatatt gaaaatcatc gctatacact
    22741 agttttttat atcggacgtc ctgcgggaac agtcttattc cagctattga tgtatgcttg
    22801 ctatttcgtg attttaatgg ggttgttgag cggtatctat gaatggccga tcttggtcac
    22861 cttcttgacc ttgccaattg tgaagaagca tttggatcaa ttcaagcaaa cgttacccca
    22921 gcctatcagt ttcagctatt ccatcaaaaa tatggtctta ttcaatgcca gttatgtatt
    22981 ggggctatta ctaacaatca ttatgcaagc catttaacag catgaagagg ttgtgaaaaa
    23041 actgccctgc atcaagtaat aagaacagcc aaataaaaat agctccttat atttttcgtt
    23101 tggttgggct tattaccgca gatgcaagct tttgaacacc gtttatcagg aggttgtgaa
    23161 aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggctttc cacatttttg
    23221 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatca gaggttgtga
    23281 aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttt
    23341 gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc agaggttgta
    23401 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
    23461 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
    23521 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
    23581 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
    23641 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
    23701 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
    23761 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
    23821 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
    23881 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
    23941 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
    24001 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
    24061 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
    24121 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
    24181 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
    24241 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
    24301 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
    24361 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
    24421 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
    24481 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
    24541 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
    24601 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
    24661 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
    24721 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
    24781 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
    24841 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
    24901 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
    24961 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
    25021 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
    25081 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
    25141 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
    25201 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
    25261 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
    25321 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
    25381 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
    25441 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
    25501 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
    25561 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
    25621 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
    25681 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
    25741 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
    25801 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
    25861 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
    25921 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
    25981 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
    26041 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
    26101 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
    26161 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
    26221 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
    26281 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
    26341 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
    26401 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
    26461 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
    26521 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
    26581 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
    26641 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
    26701 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
    26761 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
    26821 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
    26881 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
    26941 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
    27001 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
    27061 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
    27121 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
    27181 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
    27241 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
    27301 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
    27361 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
    27421 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
    27481 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
    27541 agtgtatctg cacctgcagc aagtaactgt tctgcttgtt caatagtttc tactgtggtt
    27601 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
    27661 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
    27721 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
    27781 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
    27841 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
    27901 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
    27961 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
    28021 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
    28081 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
    28141 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
    28201 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
    28261 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
    28321 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
    28381 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
    28441 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
    28501 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
    28561 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
    28621 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
    28681 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
    28741 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
    28801 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
    28861 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
    28921 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
    28981 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
    29041 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
    29101 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
    29161 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
    29221 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
    29281 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
    29341 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
    29401 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
    29461 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
    29521 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
    29581 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
    29641 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
    29701 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
    29761 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
    29821 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
    29881 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
    29941 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
    30001 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
    30061 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
    30121 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
    30181 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
    30241 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
    30301 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
    30361 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
    30421 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
    30481 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
    30541 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
    30601 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
    30661 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
    30721 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
    30781 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
    30841 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
    30901 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
    30961 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
    31021 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
    31081 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
    31141 gtttaaaaaa taaaaaagac
//
LOCUS       DADXTH010000027        29921 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.027, whole genome shotgun sequence.
ACCESSION   DADXTH010000027 DADXTH010000000
VERSION     DADXTH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.027"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(53..1117)
                     /locus_tag="IWI97_001213"
     CDS             complement(53..1117)
                     /locus_tag="IWI97_001213"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBC2462725.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1114..2199)
                     /locus_tag="IWI97_001214"
     CDS             complement(1114..2199)
                     /locus_tag="IWI97_001214"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBC2462726.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2212..3189)
                     /gene="mvaD"
                     /locus_tag="IWI97_001215"
     CDS             complement(2212..3189)
                     /gene="mvaD"
                     /locus_tag="IWI97_001215"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBC2462727.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3182..4126)
                     /gene="mvk"
                     /locus_tag="IWI97_001216"
     CDS             complement(3182..4126)
                     /gene="mvk"
                     /locus_tag="IWI97_001216"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBC2462728.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4442..5101
                     /locus_tag="IWI97_001217"
     CDS             4442..5101
                     /locus_tag="IWI97_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBC2462729.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            5186..6091
                     /locus_tag="IWI97_001218"
     CDS             5186..6091
                     /locus_tag="IWI97_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBC2462730.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(6093..6725)
                     /locus_tag="IWI97_001219"
     CDS             complement(6093..6725)
                     /locus_tag="IWI97_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBC2462731.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    6876..7001
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            7045..7569
                     /gene="infC"
                     /locus_tag="IWI97_001220"
     CDS             7045..7569
                     /gene="infC"
                     /locus_tag="IWI97_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBC2462732.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7641..7841
                     /gene="rpmI"
                     /locus_tag="IWI97_001221"
     CDS             7641..7841
                     /gene="rpmI"
                     /locus_tag="IWI97_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBC2462733.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7894..8253
                     /gene="rplT"
                     /locus_tag="IWI97_001222"
     CDS             7894..8253
                     /gene="rplT"
                     /locus_tag="IWI97_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBC2462734.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8505..9842
                     /locus_tag="IWI97_001223"
     CDS             8505..9842
                     /locus_tag="IWI97_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBC2462735.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            9890..12019
                     /locus_tag="IWI97_001224"
     CDS             9890..12019
                     /locus_tag="IWI97_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBC2462736.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11994..12683
                     /locus_tag="IWI97_001225"
     CDS             11994..12683
                     /locus_tag="IWI97_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462737.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13091..13777)
                     /locus_tag="IWI97_001226"
     CDS             complement(13091..13777)
                     /locus_tag="IWI97_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2462738.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13913..14080)
                     /locus_tag="IWI97_001227"
     CDS             complement(13913..14080)
                     /locus_tag="IWI97_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462739.1"
                     /translation="MYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKRIGG
                     RVKPIQEVRPD"
     gene            complement(14157..14597)
                     /locus_tag="IWI97_001228"
     CDS             complement(14157..14597)
                     /locus_tag="IWI97_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBC2462740.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(14601..15446)
                     /locus_tag="IWI97_001229"
     CDS             complement(14601..15446)
                     /locus_tag="IWI97_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBC2462741.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15595..17013
                     /gene="pepV"
                     /locus_tag="IWI97_001230"
     CDS             15595..17013
                     /gene="pepV"
                     /locus_tag="IWI97_001230"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBC2462742.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17070..18017)
                     /locus_tag="IWI97_001231"
     CDS             complement(17070..18017)
                     /locus_tag="IWI97_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2462743.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(18240..18590)
                     /locus_tag="IWI97_001232"
     CDS             complement(18240..18590)
                     /locus_tag="IWI97_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBC2462744.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            18790..19869
                     /gene="pepA"
                     /locus_tag="IWI97_001233"
     CDS             18790..19869
                     /gene="pepA"
                     /locus_tag="IWI97_001233"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBC2462745.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20013..20333
                     /locus_tag="IWI97_001234"
     CDS             20013..20333
                     /locus_tag="IWI97_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBC2462746.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            20355..20819
                     /locus_tag="IWI97_001235"
     CDS             20355..20819
                     /locus_tag="IWI97_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBC2462747.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            21024..21629
                     /locus_tag="IWI97_001236"
     CDS             21024..21629
                     /locus_tag="IWI97_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBC2462748.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(21668..22957)
                     /locus_tag="IWI97_001237"
     CDS             complement(21668..22957)
                     /locus_tag="IWI97_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBC2462749.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            23385..23864
                     /gene="rlmH"
                     /locus_tag="IWI97_001238"
     CDS             23385..23864
                     /gene="rlmH"
                     /locus_tag="IWI97_001238"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBC2462750.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            23949..24458
                     /locus_tag="IWI97_001239"
     CDS             23949..24458
                     /locus_tag="IWI97_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBC2462751.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            24558..25208
                     /locus_tag="IWI97_001240"
     CDS             24558..25208
                     /locus_tag="IWI97_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462752.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            25659..26597
                     /locus_tag="IWI97_001241"
     CDS             25659..26597
                     /locus_tag="IWI97_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBC2462753.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            26610..27662
                     /locus_tag="IWI97_001242"
     CDS             26610..27662
                     /locus_tag="IWI97_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2462754.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            27928..28563
                     /locus_tag="IWI97_001243"
     CDS             27928..28563
                     /locus_tag="IWI97_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBC2462755.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            28754..29545
                     /gene="msrA"
                     /locus_tag="IWI97_001244"
     CDS             28754..29545
                     /gene="msrA"
                     /locus_tag="IWI97_001244"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBC2462756.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         9316 a         5432 c         6175 g         8998 t
ORIGIN      
        1 gcttttactt ccgccgtttg tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
       61 gtcccacgct cttttctgtg cgtcttccat acttcactaa gtcgatccct cttgcttcgc
      121 accaatcctt cactggtccg gaaaagatca gagattgttg gtgtagggcc gcggtattcg
      181 ttgcgccgac catagtataa agcagacgaa gctcttcttg ccattgcttc atcagtataa
      241 tagtttcttc tacgccgtga ttcatcagat gcgtcagtac ggttcctgaa gtacccacgg
      301 catttgcgcc gagacaaagg gctttaaata tgtcataggc attgcggata cctccggagg
      361 ctaggatttc catagaagtg tctgcttcat ttgcttcaag caatgaagag actgtcgatt
      421 gcccccagtc agctaaataa ctcagttccc gcttggagcg acgggcgttt tcgatctggg
      481 tgaagcttgt tccgcttcga ccgctgatat caactgtatg aactcctaaa gcagctagat
      541 cattgagcgt ttctcttgtc atgccaaaac cgacttcttt tacgatgagg gggacgtcaa
      601 tggctgtttg agtttcttga ataagtactt tccaattggt aaaatcacgg tctccctcag
      661 gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa ggcatctgcg tgaaacaatc
      721 ggatagcttc tttggctctt tcgacagaag tccctgcacc gatgttggca atgattttcc
      781 cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc taaagaaggg actttcaagg
      841 cagcgctgac tgaaccagtg gcaatcatca aatctgcttc tcttgcgacg attgcaagat
      901 cttggttgat tttttttgtt ttttcgcttc cgccagtcat cgcattgata taaaatgggc
      961 tgcttagtgt aaacccaact gcctgtgtac ttagatcgac atcagaaact gctatttgcg
     1021 gcaaagggtt atggatgatc cgtacgaagt caaattcgtt tttttgttta tcatgaaagg
     1081 ctttagctag tgaaacatgt tcgtcttttc gattcatcgg ttttcctttc tttgatcgct
     1141 atagacatgt aacggcagtg gagtgatttc tgctttttcc catgcactca ttaaaggaag
     1201 aatgccagat ttctggtcaa caattacgat tccgcaatcg cccccacctg caccagaaga
     1261 ttttgcggct ccttcatact gttcagctaa attacacaat ttgttcaaag caggcgtttc
     1321 gatgacgacc ccagtgattg cagataaatc atgcagtaat tgtcggtttt ttcgaatcat
     1381 ctgttggatc aacgttacat tattttcttt aaaccctttg atcatttcgt tgacacattc
     1441 tgtactattt tttaagaaaa gctgataagc caccatttta tcttctctcg aacggtgaac
     1501 ttgatcgacc aaatcagaag tagaggcagg gctacccgtc caaccaatca ataaacgtaa
     1561 atcttcagga gcaatcaatg gctcaatgga tagacctggc caatccaggg caagtaactc
     1621 actgatagaa tgctgagttt cttgttcttg gagccaagga tggtcgaagg ttgagaaagc
     1681 gatccagcca ccataacagc tagcagcgat gtcgccgcag gaaccattat cttgaactgc
     1741 taaattggct agtgctgcaa tcttgaaaat ctccaactga gataaattca acgcgtaaaa
     1801 aacattcaag gctttgaccg ttgcaacggt cacggcaccg ctagaaccca atccatattt
     1861 ccggccattt gaactgtcta actcacttgt tactttcaga tcataaaagg ataaaagaat
     1921 gtttttttct tgtgcatact tttcagtcaa gcgaatagca gcaaggatat aatgaaaagg
     1981 attttcccga atatctaaaa ctaattctcc gttgcgtctt gtccaacgta caggcatccc
     2041 actatattgt gcagattgga tacttccgac ttttcgtgca gattctacag tgactgtcac
     2101 gaattgatcg actgcagcga taactgctgg atggcctgtt tcaacaactg catattctcc
     2161 ggcaatatag agtttgcctg gtgcagatac ttcaatcata aaaatctccc tttattcaat
     2221 aatcgcaatt cctggaccag catgtgcagt aattacttgt tctttgctaa ataatttggt
     2281 aaatgtttct tgtacctcag ataaatggct gttttccact aaaactttga cattagggcc
     2341 agcatccatc gtaaagtagc atggaatgcc ttgctttctc aattgtcgca ccgcatccat
     2401 cgcttttaag ctgtcaggag accagtaagt gaatggaggc tgagcagcta aagtcgttcc
     2461 atgcattttt agtccgtttc tttccatcgt ttctccgaga agttggaaat cttttgtttt
     2521 gattgcttgt ttcaattgat atagatcccc ttcaacggaa tctaaccagc cttgataaaa
     2581 actggatgtt tcgactgtcc gacgcatccc atttctgctg gacacttctt ttttctggtc
     2641 gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa gaatcggaag ggactggctt
     2701 agcgtaagaa cttaagtcgt catggccttt ttcccattcg acgaaaccac cgaaaatact
     2761 tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa agagagagat catctaatcc
     2821 aagcttaaga gcagtattgc aagcgcctgc taaagcagct agcccgctgg cagaggaggc
     2881 taatccagct gcggtaggaa cgaaattctg actgattact gaagctttta gtgagaggcc
     2941 ggcttcttta cgaaaaaggt caagaaactg actgactttt tttgttgctt tttcgtcttg
     3001 caaggtgcca tctagataaa attcatctac catatagcta tcagaaaaga tgacttctgt
     3061 ctctgtataa aatgcatcca atgttaacga aaggctgttg ttcattggaa ggatgagttc
     3121 ttcatttttc ttaccccaat atttgattag agcaatgttg gtatatgcgc gtgctttgcc
     3181 tttaaacata ggtatgtact cctaatcctt gtatccaagt ttcagcagca cccgcatctt
     3241 caagtgcttg actgatttct tgggcttttg tttttgtttg tgctaatgca atcatacagc
     3301 caccgcgtcc accgccagtt aatttggcac ccaatgcccc ggtatctttt gctgtttgga
     3361 tcaatagatt aagaaaatca ttgctgattg tcagcttttc cagaagtgat tgtgattcat
     3421 ccattgtctg tgccaacgtt tctggtgaat tttcaatgat tgcttgtttt gcttgcttcg
     3481 tcaagtatcc taatttttgg atcatttgtc cagtttcatg aggctgggtt tcaaagaggt
     3541 gagcgacatc tttgacggct tctcttgttt gtcctttgat tcctgtatca gcgacaatca
     3601 aaaaagcatc gatgttcaaa gaaaagtagt cgaaaggatg cccttttgta aaataaatcg
     3661 gctgcaagct gctagtagcg gctgcatcga ttccactagg attaccgtgg gcgatttttt
     3721 ccgaaagctg gacattttct aatagtattt cacgagacaa aggaaatgct aagtaatcat
     3781 aaaaagcacg agtgactgct gtggcgactg cagcgcttga tcccattcca cgttcagcag
     3841 gaatcgtgct ttcaatggtc agttgcaagt ttttttggat gtcatgctgt ttttttaagt
     3901 gaatgaataa ttttttgata ttttttaatg catgaggagc ttcggctaaa tttcctgaat
     3961 agtaggaaga aaccagttga tcgtccattg catccagctc ttcaaggaat gcggtgactt
     4021 ttgttgcata gaaaggaaag gcaatcgctg gttctccata aacaaccgcg tgctcgccca
     4081 tcaatatgat ctttccgctt gattctcctt ggccatagtt tgccataatc taactccttc
     4141 tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca aaacgagttg cgctgcttca
     4201 cgaatttttt tgtccactaa gggtctcaaa aaacattcct cctttatttt actaaaacca
     4261 tctcagaagt aaaactaaga atacgttttc taaagaaaaa acactttttt cttaagaatt
     4321 ttttgacttt ttttcttctt tgactagaaa aaaatacaat tgacactact agatattggg
     4381 ggtatattta aaaaggttgg agttaaacac aatatttagt gatgaagtga gctggaggta
     4441 aatgagcgtg atcgttttag caggtacaat tggtgccgga aaatcaagtt taacagaaat
     4501 gatggctgaa cactttgata gtcaggcatt ttatgaatcg attgatgata atgaagtctt
     4561 gcccttattt tatgcaaacc ctgaacagta tgcttttttg ctgcaaattt attttttgaa
     4621 taaacgtttt gcaagcatca aacaagcaat gaaagatgac aataatgtat tggaccgttc
     4681 gatttatgaa gattcactgc ttttccatct aaatgcagat ctaggaagag caacagaaac
     4741 agaagttcgt gtgtatgatg aactgctgga aaacatgatg gaagaacttc cctatgctgc
     4801 ccataaaaaa catccggatt tattagttca tattcgtgta tcttttgata cgatgttgga
     4861 gcgtatcgaa aaacgtggtc gttcgtatga acaattgtcc tttgatccaa gtctatacga
     4921 ttattacaaa gaattgaaca gacgttacga tcaatggtat gaagaataca aagaaagtcc
     4981 aaaaatccaa atcgatggag accgctataa ttttgtggaa gatcctcaag caaaagaaga
     5041 agtcttgaaa atgatcgaag aaaaattagc agagattcgc caaaccaaag tagccatttg
     5101 atttacagtt gctcaaataa agggtaaacc tttatttgag cgactttttt ctttatactg
     5161 gaaacaaatg tcaggaggat agagtatgcg ggaaaaacac tattttaatc aagaccgtaa
     5221 tttttggatc aatgtacagc aaaacgatgt gctagctgtt aaaaacttaa aagaagaata
     5281 tggcattagt gacgagatgc tgacttattc tctagataaa aatgaacggg cgcgcgtaga
     5341 gtacgattct tttgatgaag cgttactgct tgtatcaaat gttccgcacc agcaaaaagt
     5401 cgataatcat tatgaaacta gtcctatcgc ttttatttta aaagaagacg gtttgtttac
     5461 atttaccacg ccgaataccg agtacgtgat tcgactaatt cgatcgcttt tggaacgaat
     5521 gccagacatg agtgtataca gtttattgtt tcgtacattg tttttgattt ctgattcctt
     5581 ttttccttta atcgaggaag tgaatagtga acgtcagcgg cttaatctga aattaagaga
     5641 aaaaacaacg aacaagaatc tgctgcaact ttcagattta gagatcggtt tggtttactt
     5701 agttacagga acaaaacaaa atgtagtact tttagaacaa atcaaggcat tggctattta
     5761 tcgcaagttg tcagaaaagg aaaaagaaga gttggacgat gcgctgatcg aagcacgaca
     5821 agcagttgaa atgacgaact tagcttcgca gattttagac cagctctctg gcacttataa
     5881 taatttgctg aataacaact tgaatgacac gatgaaattt ttgacggtct ggtcgctgat
     5941 cttgaccgtt ccaactattg tgacaggatt cttcggaatg aatctccagt taccctttac
     6001 ccattccgtt ttcggatggg gaatcgcctt gatcatcagt ttggtcttat ctatttggat
     6061 gcttattgca ctgtggcggc ggattcgttg atctagtctt tgatctgcaa accaagggct
     6121 tgggcgatca caagtgcttg ctcttggttg acgattagtc ccgccaaaga atcttgagat
     6181 aaagcgattg ctgtgaagaa attagtcgta aagtctaatc ccttcagaga agttcgaaac
     6241 cagttcgaac cggtcaactg attgtcagac aaaaaaaggt ttttccaagt gacctcataa
     6301 aattcgctgt cttctaattg acaatgatcg aaggagacag ctttcaagtt tgtactacta
     6361 aaggaagcca tcgaagcaat acaatcagtg aaagtacagt ctcgaagata actttcggca
     6421 aaattcgtac ctgttaattt acattgatga aacacgactt ggtggaaact gccaccaatc
     6481 cactccagat tggaaaaatc acatttttca aaaatgacat tactgcattc aaaatgacgt
     6541 aaaactgttt tttgcatcgt aagtttttgg atatgactct ctcttaatac catatttttg
     6601 gcatcgagat agctttggtc acccccgttg atcagaagac cagagaagat ctcctcatct
     6661 tctaaataaa actctccctt tgtaagaaga ggaagagaag gtggaatagg ttcgattttt
     6721 ttcatgtggg ttcacctcgt ttttctttag tatagcaaag aagcgggaat cactgctaga
     6781 aagtctgcta atctaatcat tttcagaaaa ggaagacaaa ctgtatgaaa aacattgaca
     6841 aaaagattag tttttgctat actatgatgg ttgagaaata aaagcagaag cacccgcttc
     6901 tcgccttagt tgataatgtc actgggctag atagttttca ctggttctat taggtgaagt
     6961 acgcgcgggt tctgtaagag aactcgtttt tttattgccg tttattcggt aagcttttct
     7021 ttctctcata atatttggag gtgaatgacc atagcaaagg atatgatggt taacgacggc
     7081 attcgtgcac gagagttacg attgatcggt tcagatggtg agcaattagg agttaagaca
     7141 aaagcagaag cattgaatat tgcagaacaa gcaaacttgg atcttgtgtt agttgcccct
     7201 ggtgcgaaac caccagttgc gcgaatcatg gactacggta aataccgttt cgaacaacaa
     7261 aagaaagacc gcgaagcgcg taaaaaacaa aaagtgatca atgtaaaaga agttcgctta
     7321 agtcctacga tcgacttgaa tgacttcaat acaaaacttc gtaatgcacg taagttcttg
     7381 gaaaagggcg ataaagtgaa agcttctatc cgtttcaaag gccgtgccat tacccataaa
     7441 gaaattggtc aaaaagtctt gaatcgctta gctgaagaaa ctgcggatat tgcaacagtg
     7501 gaacaaaaag cgaaaatgga cggacgcagc atgttcttga cgctggcacc gaaaaacgac
     7561 agtaagtaat ttgaatgact aactggctgt tggacagccg atagtcggat aacgaaagat
     7621 tttgaggagg aaatatagtc atgccaaaac aaaaaacaca ccgcggatca gcaaaacgtt
     7681 tcaaacgtac tggtaaaggc ggattgaaac gcttccgcgc gtttacaagt caccgtttcc
     7741 acggcaaaac aaaaaaacaa cgccgtcaat tgcgtaaagc aagcatggtt tctagtggcg
     7801 acttcaaacg tattcgccaa caactagcaa aaatgaaata agaagtacaa ataacagaat
     7861 ctgaagattt atttttatta ggaggaatta tacatggcac gtgttaaagg tggaacagta
     7921 actcgtaaac gtcgtaaaag agtgctaaaa ttagcaaaag gttactacgg ttcaaaacac
     7981 actttattta aaacagcaaa agaacaagta atgaattcat acaactacgc atatcgcgat
     8041 cgtcgtcaaa agaaacgcga cttccgtaaa ttgtggattg ctcgtatcaa cgcagcagct
     8101 cgtatgaatg gcttgagcta ctcaaaatta atgcacggtt tgaaagttgc tgaaatcgac
     8161 atcaaccgta aaatgttagc tgacttggct gttaacgatg cagcagcatt tactgcattg
     8221 gctgaccaag caaaagaagc tttggctaaa taagctaaac aattataaag aggaaagaag
     8281 ataacgaaaa gttatgttct ttcctctttt tttatttaca atcctccatc aaatgaaaca
     8341 ggacaataac gtataagtct aaaatagcat aattaaggtt ttttaattga aaactgaaca
     8401 tttatggtca ccctttaatg aaagcgctaa ctacataagt tagaatgagt ttgactgata
     8461 tattagtttg cttatcagaa aaacaaaatg gacggaggaa taacatgagt gatattatta
     8521 ttggtatttt gttagttatc tcattctttt tcatggtctg gtactgtgta aaaggctaca
     8581 acttgatggt cggctttgcc atcatggcta ctgtatggat gggattagcg ctagtcggaa
     8641 acacgttttc accaaacccg gcgatggaag ggcaaggagt aattgatgtg ttgacgcata
     8701 tctatacgac ggggccagct gagtacgcca aatcgatctt ggtaaatgtc ttctttggtg
     8761 cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc taccctgatt cgaaaggtag
     8821 ttgaacttgg aggggacaag ccaagaatca cgatgagctt actatgtatt gtaaccgctg
     8881 ttatattcat gtcgatgaca ggaatcggac cagtgatttc tattgcagtt attgtgttgc
     8941 caatcttgat gtcattaggt atttcagtac cggttgcact gttctcattt atgggctcga
     9001 ttatggctgg aatctttgct aatatcgtca atttcaaaca atatcaaacg atttatgctg
     9061 gtttcaatcc agcggctgaa agctatactt ataatgacta tttccaaatt ggtatgattg
     9121 ggatgattgt aagcttagtt gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga
     9181 aacgctatgc aatggcagca aacgtaccag ctgaaggtgg cgacgcacca atgatctcat
     9241 ggttagccgt attattacca gttttagggg tcgttctttt agatcttcca atcatcttag
     9301 gttttattct agcaggtatc tgggcattgc tgtttactgg aaaattgcgc ggaggttata
     9361 aagaaatctg tcgtcagttt gcgaaattat ttacagatgg cgcagtagat gttgcaccaa
     9421 tggttggttt cttgatgacc ttagcgatgt tcaataattc agctgcgtat gcttctcctt
     9481 atttttcagc tatttttgga gattggatac cgcaatcacc attagtcttg gcaatcgtat
     9541 ttgctatatt gacaccacta ggatttttcc gcggtccaat gaatctagtc ggttcaggct
     9601 cagctatttt agctgtcgta ttagctgtta atccaacgat gtcgccagcc tttttgttcc
     9661 cgttatttgc aatcacaacg attgctcctc agcatttaga tattacacaa tcttgggttg
     9721 cttggggatt aggttataca aaagtaactt ctcgagaata tatgaagaaa tccatcccaa
     9781 ctggttggat catcggagcg atcttatgcc tgatcacgtt cttattgtac gggaacgcct
     9841 agtaaacaaa acgtcgataa aaacgattag atgaatggag gaaaagaaaa tgaaacgttt
     9901 tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc
     9961 gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc cacgatgatg tccgtggagt
    10021 agccgcaggt gtcgttcagt cttttcaaaa ttgtttgcgg gaaaataata tcagtccaga
    10081 agatgttgtg tttgttgcac attctaccac acaagcgact aatgcactga ttgaaggaga
    10141 cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa
    10201 acgacaaact agattgaacg atatcgattt agggaataag aaaaaaatag agattgtcaa
    10261 tgcattctta cctgtcaaac acttaaatgt agaccgtgtt tcagaaacga tttcttcttt
    10321 agaaagagaa agagcggagg tcttggtttc ttctatggca tttggagtag ataatgggga
    10381 accagaacga gttgtttatg aagcagcatc ggtaaaatcg atccctacaa cgatggcttc
    10441 tgacatcaca aaattatatg gattgacaag aagaactcgg acagcagcaa tcaatgcatc
    10501 tattttacca aaaatgctag atacggcaac ctctacagaa gattccgtac gagaagcagg
    10561 ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat
    10621 gaaaaaacga cctgttttaa ccatgctttc cggtccagca gcatcagtca tgggctcttt
    10681 gatgtacttg agagcgtcta atggtgttta ttttgaagta ggcggaacaa caaccaatat
    10741 cggggtcatt aaaaatggac gtccagcaat cgattattca attgtaggtg gccacccaac
    10801 atatatctct tcattagatg ttcgggttct aggtgtggca ggcggttcga tggtacgggc
    10861 taatcagtca ggaatcatcg atgtcggtcc acgttctgca catattgccg gtctagatta
    10921 tgcagtattt acggagacag aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa
    10981 agaaggagac cctgctgatt acgtcaaggt agtgatggaa gacggagaag aagtgactat
    11041 tacaaataca tgtgcagcca atgtccttgg attagtgcaa gaagaacatt tttcttatgg
    11101 aaatgtccca tctgcacgaa aagccatcca agcattagct gattattgtc atacgacggt
    11161 tgaagacatc gcagaacaga tcatggaaaa atcctatgct aagattgaac cggtgatttt
    11221 agaattggca gacaagtatc acttggagaa agatcagatt tctcttgtag gtgtaggggg
    11281 cggcgctgcc tcattgatta cttactttag taataaaatg ggtgtgaaat attcgattcc
    11341 tgaaaacgcc gaagtgattt cttctattgg tgtagcactt gcaatggtcc gagatgttgt
    11401 agaacgtatc atcccttcgc caagtaaaga agatatacga tcgttgaaaa atgaagcgat
    11461 gaataaagcg attgaatcag gcgctacacc agaatcgatc gaagtccacg tagaaatcga
    11521 tccacagact tcaaaagtaa cggctatcgc tactggttct acggaagtga aagcaaccga
    11581 tctaaccaag gagatcacaa cagaagaagc actagagctt gctgcagagg atatgcgatt
    11641 aaataaaaat gaagtttgct tattggaaaa tacacctttc ttttatgtat gcggcgaaca
    11701 gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt
    11761 ccaacgtgga catgcttcat gtatgaaaac aactgcagcc aattatatga cagctgtcga
    11821 acaactttgg gaagatatgg cagtttatca aactgaattg attgcccgac cagaatttta
    11881 tctctgctta ggagcaagag tctctgattt tacagcgacg gatctggaac aactacagct
    11941 tttgatggat ctggaagtat ctactatgga accggaggaa gaagtgatcg tcgttgccgg
    12001 aaatatcaaa caaacataag ttagaagagt tactaaacga attacaacag atccccgaag
    12061 aaatttgggg attctaccag tttcggcgag atttattttg gaaaaagatt cctttgtcca
    12121 agcagaaaat tcttatacaa caatcaatcg attgtggtat cgaaacggca tgtagcatca
    12181 aaaaaaagta cccttttgcc gatgtaggag aaatttgtga acaaatggct gttccaatcg
    12241 tttcctgtga atcagagcaa ataaacgaac gaatcacttt tgcaacttat gcagaagatg
    12301 aagggattcg attgatgacg gagccgttag aaaaattaaa atgttcagga ctagcttcga
    12361 tctctaaaga aacagcgcaa gctttgatta ttggacatga actatttcac cacatcgaag
    12421 cttctgttaa gggaatttat acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt
    12481 atacacatca atcaaacatt cgtgcattga gcgagatagc agcgatgagt ttttcaaaag
    12541 agatgaatca aagcgatttt tcgccttatg tattagaggc agtcttactt tggccatata
    12601 atgaaattcg tagccaaggg atcttagaag aaataaaaaa aatagaaaaa agatgtacgg
    12661 agtatgattt agattacaaa taaagagact gtgacaaaag tcgtgtcaca gtcctttttt
    12721 tccgaataaa cgatgttctc ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac
    12781 atgagaagct gttttttgaa attgttcctt gtgactcgga gatgaacggc aggctcacga
    12841 cctcttggaa aacggtgttc aaaagcttgc atctgcggta ataagtccag ccaagttaaa
    12901 aacataagaa gctattttta cttggctgtt cttattactt gatgcagggc agctttttca
    12961 caacctctca ataaacggtg tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg
    13021 caaaatatga ggaactattt tgccgtttct ttctctaata ctcaggtcaa aacgctgatt
    13081 ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc aaaataatcg gggtaattat
    13141 gaatcacgta ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa agaaatccga
    13201 cttcatcaaa gttatgatca atcattgatt gaagcattgt ctgatgttca tctaatactc
    13261 gaccccagtc taattctgaa atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac
    13321 tataactttc tagccaatcc caaatttctc gttttccagc aatttcataa caagttcggt
    13381 gaaaatcagt atccaaatca aaaaaagctt ttttattttg agcgattact gcctgatcgg
    13441 tttgttctag tattttttct agtttagctt gatcttgctt ggtcattttc gcactgcatt
    13501 cttgcaggat cggtttttcc aacttttccc gcgtataacg ggctaatgaa gcagatcgga
    13561 tatcgatttt tgaaacataa gtgccggact gtggaatggt ttttacaagc tcttgattgc
    13621 gcagttggat cagtgcttct cgaatcggtg tacgcccgat agctagttgt tcttctaaca
    13681 ctttatcgga gattttactt ccaggtgcta actcggaata aatgatttgt ttacgtatag
    13741 attcataagc ttgctgttgc aaattattga taaccattta accttccttt ctatgcagat
    13801 ataatctatt ataacataaa tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa
    13861 aaaaagccct gataggcatg cctattagag cctcgaaaag aaaaatattg aatcaatctg
    13921 gtcgtacctc ttgtatcggt ttgacacgac cgccgattct tttagcaaaa gcttcggatt
    13981 cttttttgtc tttgaaaatg agcagattgt ctaagttttt ttcccgagtg cgacagccag
    14041 ttttatcgat gtaaccgtgc attactttaa cgacatacat ggggatcacc tgcttttttc
    14101 ttttcattat acgaactact cgacacaata caagaaaaat gcttagacgg tctttattaa
    14161 gaatttacat catccaaaga agcgtacgct tggaataact gatctaactc cggattgact
    14221 ttgctcagct tcactaatcg ttgcgaatca gaaagtaaaa aacagtgttt actactgcca
    14281 gtagttttta attttttgtc ttcagttccc acaatttcgt agctaaaatc caaccgtgtc
    14341 cccgtatact tttggatagt tggatggatg ctcacttttt ctccgtaatg gatcatttct
    14401 ttatagctgc aatttacttc cagaacgggg atgatgatcc cagattcttc gattttttta
    14461 tagggaaacc ctagatgtac caataaggct acccgagcct cttcaaacca gcggatataa
    14521 ttgctgtgat gaataatccc catttgatct gtttcataat aaaaaggttc tcgcacataa
    14581 cctgtaaatt tctccatgac tcatgcctcg ttttccattg attttttaca aaaataattc
    14641 atccatttcg gaagtgcttc gctgatttgt gtcggcaaaa caatatattg ttcttttgcc
    14701 agctggtctc caatgaaact atgaagataa atagctgctg caatcgtttg gtcattttga
    14761 ggaaactggg ctaaaaatcc tgtgatcatc ccagctaaag tgtcacccat gccgcccgtt
    14821 gccatagcag cagaacctaa aggattggtg tagaagtgct ggtgaccata aatttttgtc
    14881 tcatgacttt tcaaaacgag cacagcaccg atttcctgtt gcttcttttt gttttcttta
    14941 tcccgttgtt cttgtatcgg tagagaactc aaccgttgcc actccatttg atgaggtgta
    15001 agtacagttt gttcgggata gggtagcaaa agatgattag cagcaagtaa acttaaagca
    15061 gagccatcaa tcaccagcca ttgttctttt gattggtttt ctagtacttg tttgaaacgt
    15121 tccaaactct tagaagaagt acccaatccg ggaccaatca agagtacatc tgcttgcttt
    15181 attacggaaa gaaactggtc agtatccgtc caaactacaa tcattgcttc tggcagatga
    15241 ctgtgaagag cactccgatt tttttcatca gttactacag tcgtgagacc cgctccgctg
    15301 tgtacggcag ctaaagcact cataatgatt gctccgccat attgttcatt gccgccaatc
    15361 aaaacgatac gtccaaatgt tcctttatgt gaaatttttg gacgaggtgt gatgactctt
    15421 ttcactagtt gttcgtttat cagcatctaa aaacctcctt ttactcatta tagcgtatat
    15481 tttttttaga gaaaaacaga taagaaaagg aattgtcaga ttttttgaac tatttttttc
    15541 tgtaatttgt ggtatcatga acacgaagac ttaaataagt ggaggattaa ctttatgaca
    15601 attgattggc aaaaagaagt agaagcacgt aaagaagact tgcttgaaga cttgaaaaat
    15661 cttttacgtg tcaacagcga gcgcgatgac tcaaaagtaa caccagatgc accatttgga
    15721 cctggacctc gcgacgcatt gaaacatatg ttagcttatg gcgaacgtga tggatttaaa
    15781 gtaaaaaatg tcgataacta tgcagggcat attgatcttg gcgaaggaga cgaaacgcta
    15841 gggatatttg gacacatgga tgtcgttcct gcaggagatg gctgggacac agatccttat
    15901 gaaccagtaa tcaaggatgg aaagatcttt gcacgcggat caagcgatga caaaggtcca
    15961 agtatggcag cttactatgc gatgaagatc attaaagaac ttgacttgaa actatcaaaa
    16021 aaagttcgct tcgtagtcgg cagcgatgaa gaaagcggct ggggcgatat ggcttattat
    16081 ttcgaacatg aagaagaacc agattttggt ttctcaccag atgctgaatt cccaatcatc
    16141 aatggtgaaa aaggaaatgt ttctttagct ttacgcttca aaggcgacaa tgcaggagat
    16201 tatgtcttga aatcttttgt ttccggactt cgcgaaaata tggtgccagg aacagcaact
    16261 gcagcacttc aagtaccaag tgcagatgca gctatcgcga tggaagaagc attttaccaa
    16321 ttcatcgaag caaatccagt aagcggaacg attgaagcgg acaatacgta tgtaaaaatc
    16381 gaattagttg gtaaaggcgc acatggtgca agcccacaat ctggtatcaa tgcaggttct
    16441 ttcttggcat tgttcttaga taactatgaa ttcctaggat ctgctaaaca atttatccat
    16501 gtagcagctg cttatgtcca tgaagatttt tatggagaaa aactaggagt cgcttatgaa
    16561 gatgaaaaaa tgggcaaatt gacaatgaat gctggactat ttgcttttga agaaaatggt
    16621 acagaggaag cgaacttcat caatatgaat ttccgttttc caaaaggtgt aacagtagac
    16681 ggattgcaaa gtgacatcga acaaacagtg gggcaagaag gcgcaacagt tactcgaggt
    16741 gctcgcgtaa tggaaccaca ttacgtacca atggatgatc cattggtggc tacattattg
    16801 caagtctatg aagaccacac tggagaaaaa ggctacgaac aaatcatcgg tggcggaact
    16861 tacggacgtt tgttgaaacg tggcgttgct tatggagcga tgttcccagg atatactgat
    16921 acaatgcatc aagcaaacga atttatgagt cttgatgact tgttcagagc aacagcaatc
    16981 tatgcagatg ccatctatcg tttggcgaaa taattaatta aaaagaggat caagaattgc
    17041 aaattatttt tgcagtcttg atcctttttt tactttctac tgtggtattc atgataaaca
    17101 tgatatagat tcaattcata ttctttctta gcgttcgtga cgactgtatc caaaatcaac
    17161 cctgttacta atgaaagaag tgctaaagtc atcaatccag tacttaaaat cgcagaaggg
    17221 atgcgtgtaa taaagcttgt cagcatatat tcgcgaatca caggaatccc cgtgaccaat
    17281 ccaaataaaa agaagaagat ggtccaaatc ccaaagaaaa gcaaaggctt ataatcttta
    17341 aacattttaa taatcatcat gatcactttg aatccgtcag agaatgtatt caattttgat
    17401 tcgctgcctt caggacgatc acgataatcg attgggattt caacgaactt gaatttttta
    17461 tctaaagcat ggatcgtcaa ttccgtttcg atttgaaaac cagaactcat aataggaaaa
    17521 cttttgacaa agatacgatt gaatcctcga tagccggtca tgacatcaag aatttttgtt
    17581 ttatacattc tagtaattgt atttctaaca aggttatttc caaaatcgtg gaaaggtctc
    17641 ttattctcat caaagtaggt tccatttgat aggcgatcac caatgaccat atctgcttca
    17701 ccagaacgta atttctcgag caaaccatgt acagcttctg caggataagt atcatcgcca
    17761 tctaccatta aataatagtc ggcatcgata tcaaagaaca tttggcggat cacatttcct
    17821 tttccttgac gaggttcttt ttttacgatt gctccacctt gaacggccaa ttcgtatgtt
    17881 cgatcggtcg aattattatc atagacataa atatctgctt ctggtaattc tcgtttaaag
    17941 tctgcaatga ctgttgaaat tgttgcttcc tcattgtaac aaggaatcag aacagcaatt
    18001 ttttcttgtt gttccatttt tcactctcct taactataga aaacaaaagc taaattaaaa
    18061 atacctcata atcaaaggaa atgctagttt ttcccgctaa atatcattta tttaaaaaga
    18121 aaatactgga atatatgttt ggaaagctct atctaaaaaa ccacccacta caaaagaagg
    18181 acatgatacc tctcttttga atagtgggtg cgtattcgtt ctctcacttt tttgacgtat
    18241 taatctgttg cacctttttt tcttgaaatt tctaatacgg ttcctgtatg agcatctgct
    18301 aaaaattcat aataaacagt ttcgccatct tctattcgag aaagacctcc ccgataagca
    18361 ctcgaccgaa cggcgaattt cctaataggt tccttttcaa aagagatcca agaaccttcg
    18421 attgcacctt cttttagaaa agatgacttg atattttcaa gtacttggtc tgctgacaat
    18481 acttttttgc gatgatacca aatcgttgaa gctaccccgc tcaataatcc tagaccgatt
    18541 ccaactgcta acccgccttt aaaatagctt agttcgcttt cttcatacat gtggacatac
    18601 ctccttcttc tcacttgtta ctccttgtgc ttattttacc acactctcat attttctttg
    18661 aaataaataa ccgctaccag aaaaaataag ctataataaa tacgatcggt ctgcagaaaa
    18721 aatcatattt tatccgcaga tcaatataag tctgagagga gtttagtctg aaatttagac
    18781 gaatagcata tggaagaaaa aacatttcaa cgaatcaaag agttaacaga attacaagga
    18841 acaagcggtt ttgaacaaga tgttcgcgct tatatggaaa gtcatatgga accgctggta
    18901 gatgagttac agttagatgg tttgggcggc atttttggtc tgcgccatca tgaagaagcg
    18961 gatgcacccc gtgtaatggt cgcagcacat atggatgaag tcggattcat gctgacacaa
    19021 atccaagaaa acgggctttt taaagtcgta cctctaggcg gctggaatcc ttacgttgtt
    19081 tctgctcagc gttttacact taaaacctct actggtaaga attatccatg tatttcttct
    19141 tccgttcccc cacatttatt gcgaggaaca agcggacaaa aatcagtaga agtaaccgat
    19201 attttgttcg atgctgggtt tgaatcaaga gaagaggcaa tgagttacgg tgtccttcca
    19261 ggagacacaa tcgttcctta cgctgaaacg atcaaaacag ccaacggaaa gaatattatc
    19321 agtaaatcat gggacaaccg ctatggctgt acaatggtat tagaagcttt agaagcgttg
    19381 caaaatgaaa cattaggtca tacattgatt gcaggcgcga atgtccagga agaagtaggc
    19441 ttgcgtggtt ctaaagcttc tgtcaataaa ttcaagccag atctgttctt tgcagtagac
    19501 tgctctgcag cagatgatac agtgacgaaa aatggcacat tcggacattt aggagaaggt
    19561 acattgatgc gtatccaaga tccaggtttg atcatgcttc ctcgtttgcg cgagtacttg
    19621 ctggatatcg ctgaaacaaa caatatccct tatcaatact ttgtttcaaa aggcggtaca
    19681 gatgcaggtg ctgctcatac acaaaacgaa ggaatcccaa gtacagtgat tggcgtagtg
    19741 ggacgttata tccatacgca tcaaacgatg ttcagtattc gcgattttga agctgcacga
    19801 gaaatgttga ttcaaacact gaaaggtttg gataaatcaa cagtgaatac gatcgtatac
    19861 ggaaaataat gcgatagtga aactataaaa tctggcaaaa gattgctttt tgccagattt
    19921 tttttataaa aagaggatca atgtttgtta gttcgaaaga aaggtgtata atttaattga
    19981 aaaatacata aacagaaagt agaggacaaa gaatgattat cccaaatagt ttagaagaat
    20041 tagcaggcta cgtagaaact ggaaagagtg tatttttctt tactgccggc tggtgcggag
    20101 actgccgttt tatcaaaccg caaatgcctg aaattgaaaa tgacttttct gattggcaat
    20161 ttattgaagt agaccgtgac aagtacatag atgtagccgc tgaatggaac atttttggta
    20221 tcccaagttt tgtcgtgatc caagacggaa aagaattagg tcgtctagtg aataaagatc
    20281 gtaaaacaaa acaggaaatc gaatcgttcc tagaaagtgt aacaaaaggc tgacatctgt
    20341 taggaggggt caagatgaat caagaatata agaatatttt agtaggtatc gacggtagtg
    20401 aacaagcact tgctgctttc aaaaaagcgg tagaagtggc tcgccgtaat aatgggaaag
    20461 tatatgtagc aaacgtaatc gatcagcaat tttataactt tatgggctat gcacctattg
    20521 atcaaaatct agtcgatcag caaactgaaa cagccaagga aatgatcgaa gaatgtaaag
    20581 aatatgggaa atctgttgat tacacaaata tagaaggaat tattgcatat ggttctgttc
    20641 gtgaatccat ggcagtcaaa ttgccaaaaa aatatgagat cgatctaatc atggttgggc
    20701 agtcaggtct aaatgcagtc gaacgcttta tgacaggaag tgtagccagc tacgtgatac
    20761 gtcattcacc ttgtgatgtc ttgatcgttt ctgacgaaga agaacagcca agtaattaag
    20821 caagtggaac ttgtacgacc tttttaatca attaactaaa acagtaaagg gactgtgaca
    20881 aaagtcttgt cacagtccct tgttacgaat aaatggtgtt ccagaagagg cttcttcgga
    20941 aataaagcat gaaaaaaatt tcatgcagtg aatgcaattt tttgttaaga taaatctcag
    21001 caaaggataa tggaggaaag aacatgattt ttgcttataa caaagcacac gtcggagaca
    21061 ccttgatggt tattatagcc gacgataaag gaacagaaaa cactgttgaa cgcaaatgga
    21121 atgttgctcg gatcaaagat gaacaaggtc aagttgttgc ttggaacttt ttccatatct
    21181 ccgatcatct aacaatcgaa gggaacggcc aagtaagctt aacagatgag gaattagcga
    21241 aattgaatcg attgatcaaa gaagcaggat tcgaagaaca gcttgaagct gatcatgaac
    21301 cgaagatcgt tgtcgggtac gtaaaaacat gcgtcccaca tcctgattct gaccatttat
    21361 cgattactga gacagaagta gacaatggta cagtattgca aatcgtttgc ggtgcaccca
    21421 atatcgaagc tggacaaaaa gttgtcgttg caaaaccagg agctatgatg ccagatggaa
    21481 tgatgatctg gccaggaact ttgcgagggg tagatagcta tggaatgatc tgttccgctc
    21541 gtgaattgca tttgccaaat gctccagcaa aaaaaggaat cttagtattg cctgaggatg
    21601 ccgtagtcgg agaagcattt ccaaaataaa gaggttgtga caagtctttg tcacaacctc
    21661 ttttttatta attttcacta ttttgatttt gttttaatgc cgattgatcg atcgtcaatt
    21721 caacagtagt tgttttttct ttcgagcctt catagtaagt gattttgaca gaatctccca
    21781 cttttttctt atacaaagca gattgaagtt ctacaccaga agaaacttct ttgtcatcga
    21841 tcttagtaat gacgtcgtat tgtttcaatc cagctttttc agccggtgta gctgtttgga
    21901 catttgtcac gatgacaccg ttagttacag atgaaggaat cttcaagatt tgttcttgtt
    21961 gctgagtaga aacggcagat aagtcgacca ttgtgatacc tagagctgga cgtgtcactt
    22021 ttccgtcttt ttccagttga ttgatgatat tcactacatc gttacttgga atagcaaatc
    22081 ccattccttc tacgcttaca ttagaagtag attcagaagt acttgcgatc ttacttgagt
    22141 tgataccgat aacttgtcct tcgatattaa ctaaaggacc accagaattt ccagggttga
    22201 ttgctgcatc tgtttggatc gcattgatat tgactgtttc gttcgattca ttcgtacttg
    22261 tcacttgtcg gttcaaggaa gaaatgattc ctgaagtgac agaatttgca tattcagatc
    22321 ctagtgggga accaatcgca atcgcaggct caccaacctt cagagcactt gagtcaccaa
    22381 aagaggcaac tgtttcgact ttgtctgaat tgatcttcaa caccgctaaa tctgaataag
    22441 catctgttcc aactaattca gcttttacct tcgtaccgtc tttcatcaag acttcaagac
    22501 cttgctggcc atctactacg tgattattcg tgactacata agcagtattc ccgtcttttt
    22561 tgtagatcac gccgcttcct tcactataag cttccaaatt actatcatct gagctgctac
    22621 tttcttcttg ctgcccgaat agtccgccaa aaccgcttga ttggttttga ctttgtaagt
    22681 tgataacaga aacgacagcg tcttgaactt tatctaccgc tttggtgata tcagaatcta
    22741 cattgacttt gacattttcc acaactgttt caccggcaga attctgattg ccgcttgtag
    22801 cagtagaact attgttgcca gtgcccatcg ctgcataaaa aatgccagct gtcagtagtc
    22861 ctccaacgat ccctccaaca agccccagac ctaacttacg ccagaggcta ttatttttac
    22921 tttttttcat ctttggtgtc acattttttc tatccatgtg ttcctcctcc aattacattc
    22981 atttatctac tgttagtata gtcttatttg ttgactttag tctactttga gactgtgaac
    23041 tttttatgaa aaactttagg aaaaatcatt gttttcagat gaaaatattt gattttcttt
    23101 ttccacagat actgtgtaaa actacgcgga taggattgga aaaataaggg ataaaacata
    23161 tccacagcct gtgtataaag tggataaatt cttataaaac gtatgtttgc ctaatgaatg
    23221 tgatgaaata aggtaaaata acactgtttt ctgtggataa acctgtggat gttgtgcata
    23281 actgttgggg aaatcttttt aacaaaaata gccaaaataa acatatagac gaaaaaattg
    23341 attataataa acaaagtgag catgaagacc aaaggagaga aaacatgaat atcaaaatta
    23401 tctcagtagg aaaattaaaa gaaaaatacc tgatccaagg tatcaatgag tatgtcaaac
    23461 ggctgaatgc ctatgcaaaa atcgaattga tcgaagttcc tgacgaaaaa gccccggaga
    23521 atttaagcga agcgcaaatg agacaagtaa aagaaaaaga aggggaacgg atcttagcga
    23581 aaatcaaaga acaagaatac gtctatgcct tagcaattga agggaaaaat cctacaagtg
    23641 aatcttttgc taaacaaatc gaccaattag gtatccaagg aaaaagccac cttgtttttg
    23701 ttatcggcgg ctctttagga ctcagcgaag ccgtcatgaa aagaagcaat gctcagatct
    23761 cttttggaaa aatgacgtat ccacatcaat tgatgcgtct gatccttgtc gagcaaatct
    23821 accgtgcctt ccggatcaat gcgggggcgc cttatcataa gtgatgcggt ttttatcatt
    23881 gatcctaaaa atcttagcaa gacaaaaagt acgtaagaaa gtaaagacta aatacttcgg
    23941 aggaacaaat gaataacaat gataggttat tgcgattgcg ttatgcgatt gatttgaaag
    24001 atacggattt gattaaagct tttgagctag gcggtgtacc actgactaaa gaagaagctc
    24061 aggcggtact gaccaaagta caggataaga acaaagataa tgcagaaaat aatgtgtatg
    24121 aaaaaaccat caacaaccaa gtttttgacg cattcctaaa tggcctgatt ctcttggcgc
    24181 gtggtccgca aaaagataag ccgatagtag acacgccgca aaaaagcaag gaaatcaaat
    24241 atatcaacaa tgtgttgcta aaaaaattaa aaatcgcctt gtctatgacc actgatgata
    24301 ttttagacgt ttttgctgag gcagaaatct atccatcaaa aggcgaaatc ggggcatttt
    24361 tgagaaaaga aggccaacga aattttaagc cttgcggaga taaatatatg cgtaattttc
    24421 tcaaggggtt aggaatttat aatcggagaa aagtataaag aaatttcatg agaaatcaaa
    24481 acaatggttg ttttgatgag agctagtttt tgtatgagat taaaatatta tcttaatcga
    24541 ggagctggat tggttttatg aatgatgaat ataagaatga tgaagataag atgctttttg
    24601 aagagataga gaatcgatgt aggctcaatt ttgagcttcg gggaaagatg tctttaatac
    24661 aacaaaaaaa gtatttagcg aataaatcag agtttactct gggacatgtt gaaaaattaa
    24721 tctcggattg gattagtagt agaagcgaat ttacaaaaat aaaacagccg attaaatttg
    24781 acatgaaaaa acttttgtta aataagagtg agataggtaa tagagatcaa tatattagag
    24841 ctaaagggca agaaataatt gattcattag gtgaaatgag gtcatacaat tatttgtatg
    24901 taacacatag agctgatgga atggtcatta ctgtggggaa atcttcttct aatgatattt
    24961 ttttagatgg agatcttttt tatcaactca atacaaatca cttgtctggc actgaaaata
    25021 taattttaag gacggaatat ggcaatgaaa tatttgcaaa atatgatgag attttaaaaa
    25081 attatttgga ctgggcttgg ataattccgg ttgagtcagg agatgctaag aaactggaga
    25141 gattattggg cgatgaacta attaataaaa aagtaccaat tttaaattat tattcccata
    25201 gacaataaaa ggctattttt catgataata ctagttataa ctgtaaaaat ggaagaaaat
    25261 ctgggaaaga agtttttaaa taagtgtatt gttcgatttt aataaataat aagcaatacc
    25321 atccgtctgt gttgcgcaac atcgtactac aatgaatatt ttttcacaaa aagaaaatca
    25381 tccatcgaat gaatggaagg ttttagtata cacgaggtta atgccccctc ttttataatc
    25441 agaggagtga ggttttttct atgaatgttt ctattttctt attgctagtc tagtttctct
    25501 tacgatgaaa ttgggattct attccagttt ttattgattt gctgataaaa taacttgaat
    25561 ggtggatata aacatgataa ataagagaag aataacaaaa caaggttgtg aaatgattct
    25621 caaatgaata taatagaaag aaaaaggagg aagaatcaat gaaaatagca attgcaggcg
    25681 ctggcgcaat gggaagtcgc tttgggttaa tgcttcacca aagtgggaac gaagtattat
    25741 taatcgatgg ctgggcagaa catgtccaac aaatcaaaga gcatggatta caagcaaatt
    25801 ttaatggtaa agaggtagaa gcaaaactac caatcgttct tcaatccgaa gtagaaaaag
    25861 aagatcaagt tgatctgatt attctattta ccaaagcgat gcagctggaa aaaatgctgc
    25921 aggatatcca atcattaatc aaaaaagata cagaggtctt atgtctatta aatggtatcg
    25981 ggcatgaaga tattattgag aaatttgtac caatggaaaa tatctatatt ggaaatacca
    26041 tgtggacggc aggtcttgaa ggtcctggtc aggtcaaatt atttggaagt ggttcagtag
    26101 aattacaaaa tttaggtgat ggaaaagaag cagctgcaaa aaaattagca gacaaattgt
    26161 ctgaatcagg attaaatgct catttttctg acaatattca ctattctatt tatcgcaaag
    26221 catgtgtgaa cggaacaatg aacggactat gcacaatctt agacgttaat atggctgagc
    26281 taggaaaaac atcgactgct cataaaatgg tggcgacgat tgtcaatgag tttgccaaag
    26341 tagcagcagt agagaagatt gaactagatg tcccagaagt cattgcacat tgtgaatctt
    26401 gttttgaccc ggaaacaatt gggttacatt atccttcaat gtatcaagac ttgattaaga
    26461 accatcgatt aacagaaatc gattatatca atggcgcaat ttctagaaaa gggaagaaat
    26521 atggtgttgc gacgccttat tgtgatttct tgactgagct tgtccatgca aaagaagata
    26581 gtttgaacgt aaaataaagg agagattgaa tggaaacttc acaaaaaata tcaccgaaaa
    26641 tttttctgaa taaagtcctc gctggtacag caacaggaat cattattgga ttgattccta
    26701 atgcagtatt aggtgccatt ttaaaatatt ttagtgcgta tccctttgcg gtaacgatcg
    26761 cacagttagc agttattttt caactagcaa cacctttgat cattggtggt ttgattgcct
    26821 tacaatttgg atttgatccg atgagaatga tggttgtagc cggagcttct tttgttggtt
    26881 ctggtgtagt caaattcaca cctgatttgg gtgaaacggg tgtttacgtt ggtgctggta
    26941 caggcgatat tattaatacg atgctgacag catcgattgc tgtcgggctg attttactga
    27001 ttggaaatag ttttgggtca gtgtcgattg tgttaacacc gattgttgta ggtgtaggtg
    27061 caggtttact tggtttttat ttattccctt ttgtaacagc aattacttct gcaattggat
    27121 cattgattaa taacttcact accttacaac cgctgttaat gtctatcttg attggttgtt
    27181 cctttgcctt tttgattatc tctcctattt caacagtagc aatcggaatg gcaatccaac
    27241 tagatggtgt ttctgctggt gcggctgcga tgggcgtagc tgcaaccact tttgttttag
    27301 tagtgaactc ttggaaggtt aataagtcag gtgtgacaat tgcgattgct ttaggcgcta
    27361 tgaagatgat gatgcctaat ctatttagaa aacctgtgat tcttattcct tgtttagtta
    27421 gtgcagtgat tacagctatt cctgtcgctc tattttctat ttcaggtacg cctgcttctg
    27481 ctggtttcgg cgtggtcgga cttgttggtc cgttggcttc tttagatgca gggctaaatg
    27541 gctttcttgt tgttctttgt tggttagtca tccctgtttt tgcggcacta attactcaat
    27601 ttttatgcga gaaagtattt aaactgtatg atcgagaaga agtcttcaaa tttttaggct
    27661 agtagatttt actaagaaat tttcaatcaa tggatgcgta agtgagatgt caacaacatc
    27721 tgacttacgt ttttttgcct ttttgagtag gagattgatg ctgacaactg aactcttcag
    27781 tgccattcga ttttttaaaa tgtagaagaa actcaccttt atgttattga ttgttcaaaa
    27841 ggcaactaga ataaaaatat gtgttaaaat gagaaaaaat ttgattgttg gatgtatcga
    27901 ataatcacta aaaagtagga gaagaaaatg atttttttgc aaaaatatgg tttttatttt
    27961 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
    28021 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
    28081 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
    28141 ttgtatcacc tatttattgg aacatcaaaa acacttgcaa tacttgctgt tgcaacagtt
    28201 ggtttatatg ggataggtga ttgtattttt actgggttat tcagtatcaa tacgaatgaa
    28261 agctcttgga atctatcgac ttggatccat aatataggtt ctgggctagg ctatgcaggc
    28321 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaagtggtca aggtacgctt
    28381 agccattttt atcttgtttt aacagttatt agtttactaa ttgcaggtct ttacggatta
    28441 gcacggattc cgtcgattag tcagtttgcc ttctttaagc aattgggctt ctttcaacgc
    28501 ttaagttttt tctttaacta tttgccgatg atttgtttaa gtttttttca actacgaaag
    28561 tagaaaaagc agtgtgaagc tgattaagta aaagaatttc tctatgggta accattcgtg
    28621 tggatgtgtt cgactaagaa tagagatgag gagagcgaaa agcaagttag tcttaacttg
    28681 ttatgcgttg tgctaaattt caggtagtca ttttttgaat gaataagaag aaaacttgaa
    28741 agagggtgac ttcatgttag atgtggagca gatggaaata gacctttata atattatttt
    28801 aaatcgaaat acaagagaat gggaaagaac acaatttgaa gaggcaaaag aagacctagc
    28861 aaaaatgtcg ataaagcagg tgatagaaaa actagaggtc aacttgcgtc cgctagcact
    28921 acgagataat ttgacaccag atgcgatgga tttttattta agaatcacag gggagtcagc
    28981 gagtgcaaag gcttatgatt atgccaaaca taagataact tcacccttat accaagaacg
    29041 ggcgattttt gccggtggct gtttctggtg tatggttgag ccttttgaaa caaaaccagg
    29101 tgtaatctca gtgttatctg gttatacagg tggagagacc aaaaaaccaa attacgatca
    29161 agtcagtggt ggatatactg ggcatgttga agctgttgag atcatatttg atacacgtca
    29221 aataacctat gaagagctgt tggagattta ttggcaaatt tcagacccta cagatgcctt
    29281 tggtcaattt caagatcggg ggaaacaata tcgttcaatt ctatttgttg ttaatgaaaa
    29341 gcaaaggcaa ttggctgaaa aatcaagaga acaactgatt caatctaacc gatttagccg
    29401 tcctatcgtt acagagattc gccaagcaaa gactttttgg ccagctgaaa atcatcatca
    29461 acaattttat cagaagcaac ctaaaagata taaaaagatc aagcattcgc atcagcttca
    29521 tttatttttt cagcgacata gatagaggaa ggttgtggcg caaagcgttc agctccgaca
    29581 aaataatgag caactgacaa aaatagcttt tcatattttt ggacagttat gaattatttc
    29641 gaggagttgc tttgagaaca ccgaggaaaa ggttgtggcg caaagcattc agcttcaagt
    29701 cgcaaggaaa caggaggtat gacaagtctt ttgtcacaac tcctgttttt ttactagcct
    29761 ttatttccat gtgaaagaga attcgaattg tagaatcttg atttttctat ggaattttgt
    29821 agagaatcat cttgataatt aatagacacc taatcgaatt ccacattaat gatgaactaa
    29881 attccgtata aaagcgcaaa caataactat aaaacccgtg g
//
LOCUS       DADXTH010000028        29110 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.028, whole genome shotgun sequence.
ACCESSION   DADXTH010000028 DADXTH010000000
VERSION     DADXTH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.028"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1031)
                     /locus_tag="IWI97_001245"
     CDS             complement(<1..1031)
                     /locus_tag="IWI97_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2462757.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IWI97_001246"
     CDS             complement(1113..1802)
                     /locus_tag="IWI97_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBC2462758.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IWI97_001247"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IWI97_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBC2462759.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IWI97_001248"
     CDS             complement(2329..2832)
                     /locus_tag="IWI97_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBC2462760.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IWI97_001249"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IWI97_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBC2462761.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IWI97_001250"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IWI97_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBC2462762.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IWI97_001251"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IWI97_001251"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBC2462763.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IWI97_001252"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IWI97_001252"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBC2462764.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IWI97_001253"
     CDS             complement(6444..7463)
                     /locus_tag="IWI97_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBC2462765.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="IWI97_001254"
     CDS             complement(7636..8541)
                     /locus_tag="IWI97_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBC2462766.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IWI97_001255"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IWI97_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBC2462767.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IWI97_001256"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IWI97_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBC2462768.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IWI97_001257"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IWI97_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBC2462769.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IWI97_001258"
     CDS             9754..10398
                     /locus_tag="IWI97_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBC2462770.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IWI97_001259"
     CDS             complement(10454..10957)
                     /locus_tag="IWI97_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBC2462771.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IWI97_001260"
     CDS             11554..12519
                     /locus_tag="IWI97_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBC2462772.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IWI97_001261"
     CDS             complement(12618..13790)
                     /locus_tag="IWI97_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462773.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IWI97_001262"
     CDS             complement(13777..14922)
                     /locus_tag="IWI97_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462774.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IWI97_001263"
     CDS             complement(14936..15868)
                     /locus_tag="IWI97_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462775.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IWI97_001264"
     CDS             15985..17064
                     /locus_tag="IWI97_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBC2462776.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IWI97_001265"
     CDS             17061..17690
                     /locus_tag="IWI97_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2462777.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IWI97_001266"
     CDS             complement(17800..18027)
                     /locus_tag="IWI97_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2462778.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IWI97_001267"
     CDS             complement(18130..20718)
                     /locus_tag="IWI97_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBC2462779.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IWI97_001268"
     CDS             complement(20738..21397)
                     /locus_tag="IWI97_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBC2462780.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IWI97_001269"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IWI97_001269"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBC2462781.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IWI97_001270"
     CDS             complement(22170..23036)
                     /locus_tag="IWI97_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBC2462782.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IWI97_001271"
     CDS             23145..23786
                     /locus_tag="IWI97_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBC2462783.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IWI97_001272"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IWI97_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBC2462784.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IWI97_001273"
     CDS             complement(25227..26126)
                     /locus_tag="IWI97_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBC2462785.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IWI97_001274"
     CDS             complement(26128..26925)
                     /locus_tag="IWI97_001274"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBC2462786.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IWI97_001275"
     CDS             complement(26903..27607)
                     /locus_tag="IWI97_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBC2462787.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IWI97_001276"
     CDS             27738..28313
                     /locus_tag="IWI97_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBC2462788.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="IWI97_001277"
     CDS             28431..29108
                     /locus_tag="IWI97_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBC2462789.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6167 c         5233 g         9144 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ttaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt agtggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa
//
LOCUS       DADXTH010000029        28995 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.029, whole genome shotgun sequence.
ACCESSION   DADXTH010000029 DADXTH010000000
VERSION     DADXTH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.029"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(42..512)
                     /locus_tag="IWI97_001278"
     CDS             complement(42..512)
                     /locus_tag="IWI97_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBC2462790.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(671..1129)
                     /locus_tag="IWI97_001279"
     CDS             complement(671..1129)
                     /locus_tag="IWI97_001279"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBC2462791.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1300..2445)
                     /locus_tag="IWI97_001280"
     CDS             complement(1300..2445)
                     /locus_tag="IWI97_001280"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBC2462792.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKRDEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2458..3375)
                     /locus_tag="IWI97_001281"
     CDS             complement(2458..3375)
                     /locus_tag="IWI97_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBC2462793.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3803..4366
                     /gene="efp"
                     /locus_tag="IWI97_001282"
     CDS             3803..4366
                     /gene="efp"
                     /locus_tag="IWI97_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBC2462794.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYGTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4852..6111
                     /gene="lysA"
                     /locus_tag="IWI97_001283"
     CDS             4852..6111
                     /gene="lysA"
                     /locus_tag="IWI97_001283"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBC2462795.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6098..6898
                     /gene="dapF"
                     /locus_tag="IWI97_001284"
     CDS             6098..6898
                     /gene="dapF"
                     /locus_tag="IWI97_001284"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBC2462796.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6901..7782
                     /locus_tag="IWI97_001285"
     CDS             6901..7782
                     /locus_tag="IWI97_001285"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBC2462797.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7779..8495
                     /locus_tag="IWI97_001286"
     CDS             7779..8495
                     /locus_tag="IWI97_001286"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBC2462798.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8501..9196
                     /gene="dapD"
                     /locus_tag="IWI97_001287"
     CDS             8501..9196
                     /gene="dapD"
                     /locus_tag="IWI97_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBC2462799.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9212..10213
                     /locus_tag="IWI97_001288"
     CDS             9212..10213
                     /locus_tag="IWI97_001288"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBC2462800.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10210..11571
                     /locus_tag="IWI97_001289"
     CDS             10210..11571
                     /locus_tag="IWI97_001289"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBC2462801.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11531..12682
                     /locus_tag="IWI97_001290"
     CDS             11531..12682
                     /locus_tag="IWI97_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBC2462802.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12657..13826
                     /locus_tag="IWI97_001291"
     CDS             12657..13826
                     /locus_tag="IWI97_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBC2462803.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14001..15380
                     /locus_tag="IWI97_001292"
     CDS             14001..15380
                     /locus_tag="IWI97_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBC2462804.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15661..16458
                     /locus_tag="IWI97_001293"
                     /pseudo
     CDS             15661..16458
                     /locus_tag="IWI97_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            16647..17315
                     /locus_tag="IWI97_001294"
     CDS             16647..17315
                     /locus_tag="IWI97_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBC2462805.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(17515..17901)
                     /locus_tag="IWI97_001295"
     CDS             complement(17515..17901)
                     /locus_tag="IWI97_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462806.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(17873..19255)
                     /locus_tag="IWI97_001296"
     CDS             complement(17873..19255)
                     /locus_tag="IWI97_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBC2462807.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(19411..20598)
                     /locus_tag="IWI97_001297"
     CDS             complement(19411..20598)
                     /locus_tag="IWI97_001297"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBC2462808.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(20605..21636)
                     /locus_tag="IWI97_001298"
     CDS             complement(20605..21636)
                     /locus_tag="IWI97_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBC2462809.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(21652..23106)
                     /locus_tag="IWI97_001299"
     CDS             complement(21652..23106)
                     /locus_tag="IWI97_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2462810.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            23281..24186
                     /locus_tag="IWI97_001300"
     CDS             23281..24186
                     /locus_tag="IWI97_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBC2462811.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24277..24954
                     /locus_tag="IWI97_001301"
     CDS             24277..24954
                     /locus_tag="IWI97_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBC2462812.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            24951..25121
                     /locus_tag="IWI97_001302"
     CDS             24951..25121
                     /locus_tag="IWI97_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462813.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            25235..26131
                     /locus_tag="IWI97_001303"
     CDS             25235..26131
                     /locus_tag="IWI97_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2462814.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(26186..27556)
                     /locus_tag="IWI97_001304"
     CDS             complement(26186..27556)
                     /locus_tag="IWI97_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2462815.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            27794..28540
                     /locus_tag="IWI97_001305"
     CDS             27794..28540
                     /locus_tag="IWI97_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBC2462816.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            28537..>28995
                     /locus_tag="IWI97_001306"
     CDS             28537..>28995
                     /locus_tag="IWI97_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBC2462817.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYIFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFF"
BASE COUNT         9364 a         5323 c         5964 g         8344 t
ORIGIN      
        1 gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac ggattcaatt
       61 gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac tttgccattt
      121 ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt accaggagct
      181 tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc aatcaattga
      241 tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc agtcgctgaa
      301 tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc ttgtgtctga
      361 taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca ttttgttgca
      421 gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac agtgccgtct
      481 tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta ttttatataa
      541 taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac tcttttcaga
      601 caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca tctttacgtt
      661 cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa aattcacgag
      721 ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt gtttcattgc
      781 ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga acatctaaaa
      841 tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg catcccattg
      901 gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc aagtgctcaa
      961 gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag cggacgtcgt
     1021 atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt acataaggtg
     1081 ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt tgtaagactc
     1141 ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag tgaaatttac
     1201 aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc aatctgaaat
     1261 attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga ttctgtcaaa
     1321 tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag aaagacggat
     1381 caagccgtcc tttatgccag cctcatctcg tttttctttt ggaatagacg catgagtcat
     1441 gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta aagtaaatag
     1501 ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa aagaaatcat
     1561 tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat cgggtaatcc
     1621 tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta ccacaaaagc
     1681 atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct gaagtaacca
     1741 gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga tctgatcggc
     1801 tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc cgcctagata
     1861 tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt gaaaataagg
     1921 agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca gagtggcact
     1981 tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat aaagtgcttt
     2041 tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta tgatcgtgta
     2101 ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc catagacatc
     2161 atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat gaatcccaga
     2221 aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa tcagttcttc
     2281 caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag gctggccgac
     2341 gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc ctgttgtggg
     2401 atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc tttcctccta
     2461 taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg taacgacacg
     2521 atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg caccactcga
     2581 acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa acccttctac
     2641 atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg gaatgaattc
     2701 tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca cagagccctc
     2761 tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg tagaagttcc
     2821 agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt ctttgatttc
     2881 atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt tattttcaaa
     2941 ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt tgctgatggc
     3001 accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg ctttcatcaa
     3061 ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta atttgtatgt
     3121 gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga tgatcgtcgt
     3181 tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc ccaatctatc
     3241 tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag aacctttagg
     3301 catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag cttctgtaat
     3361 tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag ctgactatta
     3421 aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa gttacctggt
     3481 actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt ttatgcacca
     3541 tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc cctccaagta
     3601 aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa agaaaaaaat
     3661 atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg agaccttaaa
     3721 agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt gattatgata
     3781 aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg gtatgacttt
     3841 cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac ctggtaaagg
     3901 aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat acggcacaac
     3961 gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg tacaatattt
     4021 gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc aatatgaaat
     4081 tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg aagtaaaaat
     4141 ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg tattacgtgt
     4201 tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta agccagcgac
     4261 aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg aattgttgga
     4321 aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg ctaacggatt
     4381 gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga tctgcctttc
     4441 aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat tttatttata
     4501 ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt tatgaagaaa
     4561 ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt gaagcgaaaa
     4621 tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata gcttctaata
     4681 gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa tatttcgaaa
     4741 tatactagac aattcagaaa attttgttta atattattcc aataaaattc aaatctttat
     4801 caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa aatgacagaa
     4861 ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac acctctgtat
     4921 gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg ctttgtatcg
     4981 aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat tgagatgctg
     5041 catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga aatctatacc
     5101 gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa caagacgcca
     5161 gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga taatttgatg
     5221 gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg tatcatgctt
     5281 cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc gcatattgat
     5341 tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca gttgataaaa
     5401 gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca aatttttgat
     5461 gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga ttttgaccaa
     5521 cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa tgatcagccg
     5581 atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc tttggaaaaa
     5641 cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt tggagaagct
     5701 ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa atattacttt
     5761 gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga atacgattgt
     5821 gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc aggaaaaatg
     5881 tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga atcgggtgat
     5941 cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa ctacaatcga
     6001 gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat tgttcgcaga
     6061 caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata aatataatgg
     6121 ctgcggaaat gattttatct tattagatta cgcagaagac acagattata gtcaattagc
     6181 cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg taaaaatcga
     6241 tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt gcggaaatgg
     6301 cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac aggaattcaa
     6361 cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac ctttttattg
     6421 cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta atcaaactta
     6481 tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca ctagtttatt
     6541 tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag tctggagaaa
     6601 aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg tcaactttgt
     6661 agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag taggttggac
     6721 acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa ctgggaaaat
     6781 ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct ctggtgagga
     6841 tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg aggaatgaag
     6901 atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg atcagtagat
     6961 tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga tggattatta
     7021 gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga gcagatcctt
     7081 caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc tggaacgaat
     7141 aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc agacgcactt
     7201 ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc tcattttaca
     7261 gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc tagaacaggc
     7321 atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat cattggtttg
     7381 aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat tggagaagaa
     7441 ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt aggaggaagc
     7501 ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt agtaaaagat
     7561 acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact tgaactgatc
     7621 catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc attattagac
     7681 ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa aaagaaacaa
     7741 ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga ctgattggca
     7801 gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat acggtccttc
     7861 ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg ccagaactgc
     7921 ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc ttatcatatc
     7981 aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa gaaataaaag
     8041 aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc atcatggtga
     8101 tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc gaactgattg
     8161 aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg ctgcttcaaa
     8221 caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa aaaagagaag
     8281 aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa catgatgtat
     8341 tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct aatcgaatct
     8401 ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca ggttattaca
     8461 aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc aagaaatcat
     8521 ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga atgtaaccga
     8581 gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg ttgtttttgg
     8641 tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca ttgaaatcga
     8701 aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa aaattaatgc
     8761 tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca atgccgtgat
     8821 catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga tgattgatat
     8881 gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg gtgcaggagc
     8941 tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag aagacggcgt
     9001 actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag atgcagtcgt
     9061 ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg gtggaatacc
     9121 tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat tgattgctgc
     9181 gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct gtattgtggg
     9241 ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg attttccagt
     9301 aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg tattcaagaa
     9361 aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg atttagcgct
     9421 attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa gtcagggact
     9481 atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat taatcgttcc
     9541 agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa attgttcaac
     9601 cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc agcgagtcaa
     9661 ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag atcttcatcg
     9721 tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa cggtgattcc
     9781 ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa tgataaatga
     9841 gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg tacgtgtgcc
     9901 aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt ttacgacgga
     9961 agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg atctagcagc
    10021 acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg ttgggaggat
    10081 cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg ataacatacg
    10141 caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga aaatagcgga
    10201 ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc gatgtctcat
    10261 gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg ccaagtgatc
    10321 gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga cttactttat
    10381 ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa aaggatttgt
    10441 gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga agatgaacta
    10501 gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt agtaagtaga
    10561 ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt tattgacgca
    10621 aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac agcacaggaa
    10681 atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta cggggcgaac
    10741 cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg cgcgatactg
    10801 gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg aatcatgatg
    10861 gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt tagagaactg
    10921 agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt cccagttcaa
    10981 gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca agggacaaaa
    11041 atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg acgcaaggat
    11101 tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt tatctggaaa
    11161 gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc agggatcgac
    11221 aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct catcacgaaa
    11281 gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt agctttgatc
    11341 tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt gttatcgata
    11401 ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga gttgaatctg
    11461 atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta tgaagggatg
    11521 gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg aaatcgggtt
    11581 tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg aggccgttat
    11641 ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag aagaaacgat
    11701 tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc tctcttttgc
    11761 ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg cgatgctttt
    11821 agggttgact gactttataa ctaaacacat tgaggatctt atttataatg tagtactgat
    11881 ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt caggattgtt
    11941 ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac tagctaaggg
    12001 agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag acattatcat
    12061 taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc tggaggcagc
    12121 tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag agtggaatca
    12181 tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag ctggagaagt
    12241 tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt tgaagagggg
    12301 attagaccat ttagcagaac agtttcactc tacagctact atttcttttc gctataatga
    12361 cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag attcaaacaa
    12421 actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat atacgcaaca
    12481 atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac atgatccgac
    12541 gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa cgattctttt
    12601 tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag taaataatga
    12661 tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca aaaatacgac
    12721 aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg ggagaaccgg
    12781 attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa gaagactttt
    12841 ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt atatttcagc
    12901 aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca gttggtgcaa
    12961 cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag gttcttattc
    13021 cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct gaattgatcc
    13081 cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa cgagcatttg
    13141 aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat cctactggga
    13201 cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa tatccggtat
    13261 ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat atgtccatag
    13321 ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca catgcgatga
    13381 ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa ctaattaaag
    13441 tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg gctgaagcgc
    13501 taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca cgaagagatt
    13561 atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac ggtgcatttt
    13621 acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt tgtgtagaat
    13681 tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca gaaggagaag
    13741 gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc tgcaaacgga
    13801 tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca atcgttaatt
    13861 ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat gaacgcgttt
    13921 cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat tttaaaagaa
    13981 tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt gcatctcatg
    14041 gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta gatatcacgc
    14101 tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg tatttagaag
    14161 acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt ccgcaatcca
    14221 atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa aaaacagtaa
    14281 cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa ttgattttga
    14341 gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa aatgtctact
    14401 tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat ccggctatta
    14461 aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa gcaagccgaa
    14521 aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc acttatttag
    14581 atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt gaagttttga
    14641 caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc aaaacaagtg
    14701 aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca aatacagaat
    14761 ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat gaatacttgc
    14821 aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat tctatcccag
    14881 cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca cgttatgtag
    14941 taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta ggttcctctg
    15001 cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt acagcagcaa
    15061 aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca aaatatgtac
    15121 cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca atgacccatc
    15181 aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga aatgtattgg
    15241 cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac ttcttcttcc
    15301 agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa gcgttaggag
    15361 aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga aaaagctgcc
    15421 ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt aacttggctg
    15481 gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa gggactgtga
    15541 cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt ggaggtcggt
    15601 attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg attatttgga
    15661 tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga aatgcttaaa
    15721 attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact aagaaaccgc
    15781 ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg aataaaagta
    15841 acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt tggagcagta
    15901 gccaaaaatc ggatccatcg tcgtttctac accagtatct gccatcagaa aatcacgacg
    15961 gatacaactg aatttaaata ccatgaagtg gatgccaaag gcattatccg tcaaaagaaa
    16021 ttgtatctag atccttttat ggatatgttc aatagtgaga ttttatccta tcgtatctct
    16081 gaaaagccca atgcattagc ggtaatggaa ggtttggagg aagctatcca gaccactaac
    16141 gattgtccct atcgtcgtac gtttcactca gatcaaggtt gggcgtatca aatgaaagct
    16201 tatagaaaca aattgaagga gtacaaaatc tttcaaagca tgtctcggaa agggaactgc
    16261 ttagataatt cacttatgga gaattttttt ggcttattaa aacaagagat ttttcacggg
    16321 aaagtttata actgtttcga ggaattaaaa tcagccatcg atagctatat ctattactac
    16381 aataatgaac ggataaaaca gaaattaaac tggcaaagtc tcgtccaatt tcgtaaaaca
    16441 accgtaacgg ttgtgtaaaa atatcatttt agagcataaa aaaaaggagt ggatttctcc
    16501 actccaaaaa agtctaactt ttgggggtca ctacagtgtc acagtccttt ttttagtaaa
    16561 aggatacatt tcttccttgt atattccatg cgtgttcgtg agaaagcata tataatttaa
    16621 ttaacagata gttaggggga gaaaaaatga ttttttcaat tacaatgaaa ctggtgttcg
    16681 ggttgatcgg tttattatta gtggtacgtt tgcttgggaa gaaatccatg tcagaaatca
    16741 caccgtttga tttagtctat acgcttgttt taggtgggat cttagaagaa tcaacctatg
    16801 atgacaatgt ccatgtaggg catgtcctat ttgccattgc gctttgggca gtaatgattt
    16861 atggtatcga acgtatcgtc caaaaaaatg aaaaggtcaa tcgttgggtc aaaggcgagc
    16921 catctgttct aatcaaagac ggcgtcatca acatgacaga gttgacaaat aaccatatcg
    16981 agatggagca gttgcgagca atcttaagac agcaagaatg cttttttctt gaaaatgcca
    17041 aacatgttat cctagagaat gccggtcaaa tgagtgtatt gaaaaaatca gatgaagaca
    17101 aagctttatc gatcctgctt gtagatgaag ggcaaattca gcataaagtg cttcagtcca
    17161 atcaattgac tgaagcatgg cttatggaga acttgaagaa agaaggctat gcagatgtaa
    17221 aacagctgat ttatgttgaa tggtcagaag aaaaagggct ctacatacta aataaagaag
    17281 acgtcatgca tgaatcttac cgaatagatg gatagataga cagaggttgt gaaatcagcg
    17341 ccttggcttg ttactcaaaa ctaagcgact ttttcacaac ctctgatatc taatatacgt
    17401 tgttccagaa gttgcttctt cgaaaaataa gccaaaattc cacaaaaatt taagaagcaa
    17461 ttttcgaaaa atcctttctt attttttgaa gttgatcact tctgtcacaa cctctcatgt
    17521 cacaaaccat ttttttgttt cctttaactc aatataccgt acgtcttccc acaatatcgg
    17581 ctgatggtca atcataaagt aatcttcata ggcttcacca atgaagtgcc cttcgacctc
    17641 atccaagagt cttccaaatt catcttttaa attgagctga atgattactg ctttacgaga
    17701 taaaaaggct tctgtcagta tactatcgat ttcttgcaat gtcatttgtt gtttaggaag
    17761 aggctgtttg cgtgcttctt cttcattttt tttgataccg ttgactagtt cttccatcgc
    17821 aaaagcagtc ccccatttta atccaaatgg ccgatctcga tactcgttat agtcaatgaa
    17881 tttttttgct gtccgtctca tcttttccac cttctaatcc actcatacca cctgcatgcc
    17941 cgccaacgag agaactacgt gcgatggcgc gtcctccttg caagagacta tgcgcatgga
    18001 tgattgattg aaagccgtat ttttttctaa tcgtatcgac aacaaaatca actttcgctt
    18061 catttatctg cttttcagga tcttcaaaaa ggttaagctg taaacctgtc gcgtatacga
    18121 ggtcggaaca gttcacgcct acacgccgaa tatcctggta gtggtaatgc tggtggaaaa
    18181 tggtcaataa agcatctgct agatctctgc tagcatttgt cgcttcgatt ttcatttgct
    18241 ggtgaaaccc ggtcttgcca tttggatcaa gatagcctaa agagtatcct acccataagc
    18301 tgactacttg tgtttttgct gcagatcgtc tcaagcgagt gccgacttgg tcggccattt
    18361 ctttgataac gatctcgatt tcttctcgtc ttgtataatc tctatctagg atttgactat
    18421 ttccgatact ttttgatttt ggtgtatatt cttgtcccaa aaaacttcga tcgatgcccc
    18481 aagcatgggc ataaagctga gtaccgatga cccctagctt gcttttcaag tgataataat
    18541 ccgcgtgagc taaatcataa acagaatgta tacccatttg tttcagacgt tttgctgttc
    18601 gtttgccgat tccccaaaag tcagtgatat ctgagatttt ccataacttt tctggtacat
    18661 cttcataacg ccattcagca aacatttgct ttccgtcttt tgagccattg tctaaggcta
    18721 gtttagcaag tagcgggtta tcgccaattc ctatagtcgt gtagattccg gtctgctcaa
    18781 acacatcttt atggatcagt ttagccatgt cgtacgcatt tgttttacca aagagtgcca
    18841 tgctatttgt tacatcaagg aagctttcat cgacggaata gacatgatgg ttcgcttcgt
    18901 ctgcatattt tttatagata gaattgattt ctctgttctt ttccatataa taggccatcc
    18961 ttggggcaac aatatataaa tcacttggat aggggaaggg aagatctctg gcacggctga
    19021 tattggtgat gccgtaagct ttttttgctg caggactgga agccaagatt aatccgctac
    19081 cccgtgtttc tgtcgaatcg gacggatagg acatgactac taatttagtt gtaaggggat
    19141 caagatcctt ttcaacgcac tctacggagg cgtaaaaaga tttgcagtca atacataaaa
    19201 tatctcttga aggttctttt tcataaacaa ataccggatt ttgattcgcc cacataacaa
    19261 tcacctcttc aacacaatta gaacacacgt tctctttccg tgtcaataaa aaacagcaat
    19321 tttcaaatac gagcaaaagg ctcacatttg gaaattgctg agaggttatt tttatctatt
    19381 tgtttttagc tgagatcgtt gattttttta tcaaacatga acagcttctg gatcgatctt
    19441 tgttgtccca tttccaatgt gtgcaacata aaattccgga gcatagccga caactttcat
    19501 gtattcatca tagacattgt ttttgaaggc aggtatattt tcttctttga caagagcaat
    19561 cgcacagcca ccaaatccag ctcctgtcat tcgtgcacct aagacacctt cttgtttttg
    19621 agcagcatct actagtgtat ctaattcgat cccagttact tcataatcat agcgaagaga
    19681 gtggtgagaa tcatttaaca gttgaccaaa tgcttctaag ttcccagctt ctaattcagc
    19741 ttttgccttc aaagtccgct gattttcaga aacggcatgg cgagctctgc gtatcaatgt
    19801 atcatcgccg atttggtcaa tagttgattc aaattcttct tcatctaagt ctcctaaaga
    19861 agagataggc aaagtttttt gcaaacgtgc caaggcttct tcgcattcgc tacggcgttc
    19921 attgtatttt gaatctgcca attcccgccg cttattcgta ttcatgatga cgattgcata
    19981 accatctagt ttcactggaa ccatttcgta tttcagcgta tttgtatcta ataagattgc
    20041 atgatctagc tcaccgaagc cgatggcaaa ctgatccatg atacctgaat tgacaccgat
    20101 gaattcattt tctacttttt tacctgtttg gactagttcc aagcgatctg tttttaaatc
    20161 aaacagatct tctaatacaa cacctactaa taattctaat gaagcgctac tggaaagtcc
    20221 tgcaccatta gggattgttc cttccactaa taactcaaac ccatgatcga ttgagtgtcc
    20281 agcttctttt aatttaagga ccattccttt tacgtaattt gcccagtttg cttttttatc
    20341 atattctagc tcatctaaag taaaactaat gacaccgata tcgtcaaagt ttgtggaata
    20401 aactaatatt ttattatcgt ctcgaggtgc tgctacacct tgcgttccat aagtgatgga
    20461 agcggggaag acatggcctc cattgtaatc tgtgtgttca ccaatcaaat tgatgcgtcc
    20521 tggtgagaag tattgtgtag tttttttagg tccgaatgtt tcggcaaatt ttgtgtttaa
    20581 gcttcttttg atttccatga tgagttattc ctttcttgtt ttgattttga attctgtcac
    20641 tgtttcaaat actgatccgg cttttaaatg aatcgaacca aatgctgaga agcgctctgc
    20701 acctggagct gtttgagtct caaatgtgat gccaccgtga tgtgccagtt ttcggttgcg
    20761 catttctggt gtttctgtgc cgaaatttgc agtgaaaata accacactag aagcatctgt
    20821 agcaatatct acttggattc ttttatcggg gctagtcaag cgagcggctt ctttacccaa
    20881 gccggtttct tttaagaaaa atggatgatc gattccgtca acaagatttt tttgatccaa
    20941 atcactagca aaaacggacg ctaatttttt aggtatttga aaatcgaaag cacttcctgc
    21001 agcattttct tttaccccta taggaatact atctgttcgc aaaggagcat aagcttcgct
    21061 attgagccaa agctcatgtt gatcgattga ctgagaagca tctcccgtca agttgaagta
    21121 aacatgattt gtcggattga ataaagtatc ttggtcactg gtgccgcgag aggtcactcg
    21181 ccagatatta tcttttgtca aagtatagcg gatttccaca tccattgtac ctggaaaacc
    21241 atgttctccg tcgggacttg tagtagtaaa aatgacagaa gcttcatttt cgccattcaa
    21301 aatgacataa gaccattttt ttaattcaaa tccaggagca ccaccatgca gattatgtcc
    21361 agtttttgga tctgtggcta attggtaagt ttttccattg agagaaaagt ggccattttc
    21421 gattctccct gcggtgcgac cgatagaagc accgatatac ggatcttttt cgatatattc
    21481 ttcagcggtg tcaaatccca aaactaattc tcgatcattt gattttaaac tgacgattct
    21541 agcacctaaa tcagagatag aaagtgctag cttgtttttg ttctccaagg ttatcaagtg
    21601 ataaccttgg ccaaaatctt tttctgtaat cttcattttc aagatccttt cttaatggga
    21661 aggaactact gcatcgtgaa gttcatcttc cattgtatca gctggagcca atttgttttc
    21721 caatttctca acgatttgtg catgtttttt ctctgtcaat gtaatcttcc aagcaaaaat
    21781 aatagcggag aaaatcatca aagcagcagg agcatagaat gcataagctt tgaaagtcgt
    21841 tacgccatga gcagtgatat cagcagcagt agcattacca gtcatcccag aagcgctagc
    21901 gacaaaccct acgatcccgt tagacaaagc accagcgatt ttatctagta aagggcgaat
    21961 tgatagagta acagcttcgt tacggatacc atttttccat tgaccatatt caacagagtc
    22021 tgtgatcgtc atcaaagcgg ataagaaaat caattgttga gggaagtaga acaatactag
    22081 cccgatgata acgattggca aacttgaacc tgcgatcgta aagaaaaagt aagaagaaat
    22141 catcattgcg atccctccaa ggtaaacgta gcgacgagag atgacgcgtg taagcattgg
    22201 gaatagtgga acagctacta aaccaagtaa ggcagcaact agaccaacga tccaaaattc
    22261 agttggttgg ccaagtacat atttgaagaa gtagaataat acggcagttg ttgctacatt
    22321 ggcaatcgca taagcgatat aagcaagggc aagccacatc aactgatcat tttgagcaac
    22381 tgctttgaac acatctttga tcccagcttt ttcattttgc tgacggatcg cagtatcgac
    22441 ttcttttgtg cccaaggcag tgataatcgc agtaccgcct gaaataacag cagcaatcac
    22501 accgaaccag aaccagcctg aagcgccttc aatatgttta tgagtaacaa gataggtaaa
    22561 gaaagtaacg atagggacaa cgactagagt agtaccattt gcacctagag aagagccgaa
    22621 acgagcaaac gtagcaaatt tttcacgttc ctttgtttcc gtactcaaag ttggaatcat
    22681 tgaccaaaaa gcgatatcct taaatgaata aaaactatcc aataagatga aaacgattgc
    22741 aaataatatg ataaataaag tcgtattatg agtagctaag ccaaagaaat tggtaaagat
    22801 tgctaccaag agtacggaac taacaacgcc gccgataact aaccatggtt tgaatttacc
    22861 gtattttgta cgagtattat cgatgatgcc accaatcaaa ggatcaaaaa taatttcaac
    22921 taagcggata cctacaacta agcttgtaac tgtagcaatc atttttgcat tcgttgcctt
    22981 atctgttcct gcgaacatct ggcttgtcac gaataccata aaataagtgc ttaatgtaac
    23041 atagaatgtg tcgtgtccaa aagcgcctaa agcgtaggac acacgttgga tcattttgct
    23101 tttcataatc tgtattcctc tcctgttcaa tatataatac taagtaaacc ggttacaacg
    23161 aataacaaag atcttttaat gacttaaact taacaagaaa tcgcttcctt tttaatatat
    23221 ttctgtgact tttatttaac tttttgttgc attatactaa aggagtaagg agggatagac
    23281 gtggagcaat cgattttttt aaaaaaggaa catcaacaag tagaaaaaga attgtatttt
    23341 gatttttgtg gtttctctaa aacgatgccg tttcattctt ttggtcctgc tattcgcaat
    23401 gattacatcc tgcatgtcgt gatggaaggc aaaggatctt atcatgtgaa aaatcaacaa
    23461 ttccaattga aaaaaggtga tctgttcttg attcgtccag gagactcgac gttttactta
    23521 tcagatggag aagatccgtg gatgtattgc tggctttcat ttggtgggag tgctgcagaa
    23581 gaaatcattc ggcagtcgct tttcagagaa gaccaataca cgatggtctc ttctgacata
    23641 caaaaatata tcgacattat tttagaatgc atgaaagata cggaagaaac cttggaaaat
    23701 gaattacagt taacagcatt aacctaccgt tttttatcag tccttttaaa agatggcgga
    23761 aaggtcagcg gtggtgtaca aaaggttcat tcctctttag ctgttgaaac agtcaaatac
    23821 atcgaggcac attatgctga aaaattgtct gttgaggaaa tcgcccagca tttagcggtc
    23881 aatcggagcc atttgtcgcg agtattcaaa aatcatatgg gtaccagtat caaggaatat
    23941 ttgatcgggg tacggatcaa tcgaggggcg tttcttcttt ctctgacggg tgaatccgtg
    24001 gaaagtattg cctatcaagt agggttcaat agtctagtcg tatttagccg aatgttcaaa
    24061 aagagtaccg gagaaacggc tacgaactat cgtaaacgta tgaaaaacga aaattttaag
    24121 aatattgctt tggatacctt gaaaaaacag ttggaaaaac aaaccgcagt ttcttggaca
    24181 acctgattag tctggttaaa atgacaagca tcggcaaaag aaacttgtgg cggttatgct
    24241 acactgtaaa gcgaagacta aaaaggagga aaagggatgc gtacacttat catcattagc
    24301 catccaacga tcgaagaatc tttaaatcag caatttttca aagaagcttc tgctggttta
    24361 ccagctactt ggcatcattt ggaatcaagt tatccagacg gaaaaataga tatagagact
    24421 gaactacagc tcttggccca gcatgatcgg atcattttcc agtttccgtt ttactggtat
    24481 agttctcctg ctcatttaaa aatttggcaa gataatgtct tggaacgagc agcacatgta
    24541 ttaaaaggga aagaactagg aatcgtattg acaacaggtg tggcggaaaa agaataccaa
    24601 gctggaggaa aagaagaata tacgatttcc gaatttttga ggccgtatca gcgaatcgca
    24661 aataaattcc atatgaccta tcttccgcca ttcgttttag cacaatttat gtatttatcc
    24721 caggaaaaac gttgggagaa gctaattgct tatcagcagt atctctcatt ggaaggcaaa
    24781 ccatcgctga ctcagcggat tgactggttt attcaaagag tacaggaaaa tcagaaaatg
    24841 caggaagaag aatctgaaaa acaagcatat atcgtagaag cactgactga ggcaaaagag
    24901 cagatcgaag acttgacctt tacactgcaa gaaatgaaag gaacttcatt atgacaaatg
    24961 attggctgat ccaacaatta gatgaattaa gcgaaaaatc agaatatcaa gaccgtgcgc
    25021 tcttatatga aataaagaaa ctgatggctg agatagacga acgaacaatg cagctgcaag
    25081 gagaaatcga cggacaatct tggaatcatc tcaactggtg aaattttctt ataaataaca
    25141 aagataaaaa tgtgcacagc gacaattatt tgctacaata agaatatgaa gcgtttgata
    25201 acgctattat acataaaaat tggtggtgaa aaaaatgtat gatgaaaatg agtggggaga
    25261 aaatgttcgg atgacccgaa gaagacagca aaaacaggca gctcgtaagc ggatgatcat
    25321 gattggggca gcagttgccg tgtttttgat catagcagga ggagttgcgg gtctatattt
    25381 tcatggaaaa aattcgtcag aagcgaaaag tacgaccata acggaaacaa cgacaaagaa
    25441 aacagagacg aaacaaacaa gtgagtcatc aactaaaaca aaagagacac aaacttctga
    25501 agaaaaaaca gcaaatggtt tgccagagca acttcaagca atcgtaaatg attattcgaa
    25561 aaagatcgaa gaaaagacgc ctgcattgat tgaagaatac cagacagaaa ttcagggaaa
    25621 tcaagaggga atagctggtt tatcagcagt tgctaaccaa aaagcacgtg agcttcaagc
    25681 aatctccgac gaagggatca gaaaattacg tgcagcgtat caggcagcag aaaataaaga
    25741 cggtgtagac ttagatactt tgatcaatca attatcggct aattatacga atcatgtcgc
    25801 taagatttcc gatatttatt tgcagactag cgcctctctt caagcagagt caacaagcac
    25861 acaagacaca acatcttctg attctgagac agcagagtcg gtagaagctt ctgaaatgca
    25921 agctcgaaca acacaagaca caacagattc ctctgaaact aataccaaca gcgcgacttc
    25981 acaggctgcg acaaccgtag ttttggaagg agaaggtcca aatcaaatag ccgaacgaac
    26041 tggcgtacca gtagaaacga tcttgtcgct gaatgggatg agcatggaga attatttcct
    26101 gactcccggt gagacgctaa aattgaattg ataaaagcaa taaaaaagaa cgaagcttcc
    26161 actaaggtga agcttcgttc tttttttatt ttccggttct tttttcgcgt acaaagtaat
    26221 ggatcgtcat gtaaagcaag aaaacgagtc caataatcaa tgagattctc gtttcaggat
    26281 tgataaacat gaaaatcaat acgatagcta aaaaagcaag tgtcacataa ttcgtataag
    26341 gagatagtgg cattttaaaa ggatgatcat gcattttatc tgcgtgcttc tttttgaagc
    26401 gaatctcgct gatcaaaatc actacccaag gaaccatacc aggcaaaata cttgagctgt
    26461 atacgaaaac gaaaatattt tctgactggt tgatgaacat tggtagaaga taatttaaga
    26521 tcagaccaat taagattcca cctgagattg ctacgacagg ataaaaaggc gcaccgtgac
    26581 gagatagttt cagaaatttt ttagacatct ttccatttat ggcaagtgta tagaccatac
    26641 gacttgcgct gaaaatccct gaattgcaac ctgataatgc cgctgtaatc acgacaaaat
    26701 taatcagtcc tgcagcaaat gtaatgccga ctttagaaaa agtttgtaca aaaggtgaac
    26761 caatctcact caattgattc cacggataga tactcacaat gataaaaata gcaccaatat
    26821 aaaaaatcaa gattcgaccg atagtggact ggacagcttc aatgatcgtc tttttaggat
    26881 tctctgcttc accagcagtc attccgatca gttcgattcc ttgataggaa gcaacaacga
    26941 tcgataatgc aaagaaaaat cctttgacgc ctccagtgaa gaatccacca tttttccaaa
    27001 gatttgaaat ccctactgcc tgaccatgat taccaaatcc aaataagatt accccaagac
    27061 ctgcaatgat catcaaaatg atcgttatga ctttgatgat cgaaaaccaa aattctaatt
    27121 cgccaaacgc cttgacagaa ataagatttg ccagcataag aaacaagacg acaatgattc
    27181 cgggaataat tgccggcaaa tcaggccacc aataattcat ataagatcct acagcaatca
    27241 cttcacttat tcctacgaca atatattgaa aaatattact ccaagcggtc aagtagccag
    27301 ccacaggatg gatgtactcg ctcgcgtaat ttgcaaaaga accagtcgaa ggatcaacgt
    27361 aaagcatttc gcctaaagca cgcataatca aatacaaaaa gaatcctgca atcgcatagg
    27421 ctcccatcac tgagggccct gtccaacgta tggttgagga ggcacccata aacagaccga
    27481 caccgatcgt tccacctagt gcgatcattt ggatatgacg ggcactaaga cttcgagtta
    27541 acttttcttc ttccatacaa aaactccttt cgttgttgag tcaacaagaa aaaataatca
    27601 aacgtttcaa cacatgtttt tcattattag gttagcctta ttgaactctt gatgtgattc
    27661 tacagaaaat tttcataaaa tgcgaggaaa attcgaaaaa aactttttca tcattctact
    27721 tgaaaatgct ttccatttta gttaaactat atttaaataa aaagttaggt aaacctaaaa
    27781 aggagtgtaa gatttggaac gtacggccat tgtaattcaa gacctttcag ccgcttatca
    27841 agggaaaact gttttgaaaa ctatcaactt gacgataaat ccacaaaaaa ttaccggtat
    27901 tatcggacca aacggtgcag gaaaatcaac atttatgaaa tctttgctag agttgatacc
    27961 agctactact ggtaaagtca ccttttctgg acagtcggta aactcagtcc gtaaaaaaat
    28021 agcctacgtg gaacagcgaa gtgaattgga tctttccttt ccaattgatg tattaggcgt
    28081 tgtactttta ggaacatatc catctttacg aattggacaa agacctggga aaaaagaaaa
    28141 agaacgtgca agacaagctt tgaaaaaagt agggatggaa gaatatgcca aaagacagat
    28201 cagcgaacta tcgggtggac agcttcagag agtttttatt gcaagagctc tagcccaagg
    28261 agcagaatgg atctttttag atgaaccatt cgtagggatt gatgcgttaa gtgaacgaaa
    28321 gatctttgac atcttgcagg aattgaagaa ttcaggaaaa acgattttga tcgtccatca
    28381 tgatcttcat aaagtagaag aatatttcga tgaggttatt cttttaaata aacagctgat
    28441 cgcttccggt ccagtacaag aagtctttac atcgaagaac cttcaattgg cctatggtga
    28501 gatcatcaga catttagtaa aaggaggaga gaaaaaatga tccaatcctt tattgatgga
    28561 ttaatgaatt atcaattcct gcaatacgct ttggtcactt ccatgcttgt aggacttgct
    28621 tctggagtca tcggttcttt tattatttta cgagggatgt ctttgatggg agacgctatc
    28681 tctcacgcag ttcttccagg tgtagccatt tcttatatat ttggttttag ttacatcttt
    28741 ggagccactg cttttgggat gctaacagca gcagtgattg gtttcgtgac tcagcggagc
    28801 cgattgaaaa atgatacagc gattggaatt gtctttagct ccttttttgc attagggatt
    28861 atcttgattt catatgccca gagtgcgaca gatttgtatc atattttatt tgggaatgtc
    28921 ttagctgtta gggaaagcga tttgttgctg acagcactcg ttagtggaat cgtcttgata
    28981 tttgtctttt tcttt
//
LOCUS       DADXTH010000030        27915 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.030, whole genome shotgun sequence.
ACCESSION   DADXTH010000030 DADXTH010000000
VERSION     DADXTH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.030"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(426..1370)
                     /locus_tag="IWI97_001307"
     CDS             complement(426..1370)
                     /locus_tag="IWI97_001307"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBC2462818.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1666..2610)
                     /locus_tag="IWI97_001308"
     CDS             complement(1666..2610)
                     /locus_tag="IWI97_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462819.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2831..4045
                     /locus_tag="IWI97_001309"
     CDS             2831..4045
                     /locus_tag="IWI97_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2462820.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4067..6082
                     /locus_tag="IWI97_001310"
     CDS             4067..6082
                     /locus_tag="IWI97_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HBC2462821.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6165..7538)
                     /locus_tag="IWI97_001311"
     CDS             complement(6165..7538)
                     /locus_tag="IWI97_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBC2462822.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7564..7848)
                     /locus_tag="IWI97_001312"
     CDS             complement(7564..7848)
                     /locus_tag="IWI97_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2462823.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7850..9865)
                     /locus_tag="IWI97_001313"
     CDS             complement(7850..9865)
                     /locus_tag="IWI97_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBC2462824.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10044..10349)
                     /locus_tag="IWI97_001314"
     CDS             complement(10044..10349)
                     /locus_tag="IWI97_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBC2462825.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10438..11469)
                     /locus_tag="IWI97_001315"
     CDS             complement(10438..11469)
                     /locus_tag="IWI97_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2462826.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11676..12551
                     /locus_tag="IWI97_001316"
     CDS             11676..12551
                     /locus_tag="IWI97_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBC2462827.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12703..13545)
                     /locus_tag="IWI97_001317"
     CDS             complement(12703..13545)
                     /locus_tag="IWI97_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2462828.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13637..15514)
                     /locus_tag="IWI97_001318"
     CDS             complement(13637..15514)
                     /locus_tag="IWI97_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBC2462829.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15511..16932)
                     /locus_tag="IWI97_001319"
     CDS             complement(15511..16932)
                     /locus_tag="IWI97_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBC2462830.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16935..20324)
                     /locus_tag="IWI97_001320"
     CDS             complement(16935..20324)
                     /locus_tag="IWI97_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBC2462831.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20581..21972
                     /locus_tag="IWI97_001321"
     CDS             20581..21972
                     /locus_tag="IWI97_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462832.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22045..22692)
                     /locus_tag="IWI97_001322"
     CDS             complement(22045..22692)
                     /locus_tag="IWI97_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBC2462833.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22700..23386)
                     /locus_tag="IWI97_001323"
     CDS             complement(22700..23386)
                     /locus_tag="IWI97_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462834.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23392..24465)
                     /locus_tag="IWI97_001324"
     CDS             complement(23392..24465)
                     /locus_tag="IWI97_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462835.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24687..25361
                     /locus_tag="IWI97_001325"
     CDS             24687..25361
                     /locus_tag="IWI97_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2462836.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25366..26706
                     /locus_tag="IWI97_001326"
     CDS             25366..26706
                     /locus_tag="IWI97_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2462837.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26855..27841)
                     /locus_tag="IWI97_001327"
     CDS             complement(26855..27841)
                     /locus_tag="IWI97_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBC2462838.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8139 a         5275 c         5059 g         9442 t
ORIGIN      
        1 taaaaaatga taaacctctc tcgaacggtt ttttatcaaa aatcgtcatt tttccatcaa
       61 aatctgctta tgcaaaatta taaaaaaatt ttgcagtttt tcttactttt tttgtcgttt
      121 aattgaatga taaaaaccag tttctttatt cagttaatca aatatgaacg aattagatat
      181 gaatcattgt atttgccgtt gcggaaggat tttttaatta accacagata aacattgata
      241 acgattcgct tattgtaatc aacagattaa atttcatatg ttcatatgta cggatacatt
      301 ttcggaccga ttaattttaa ccattctgtt tatttgctta cctattagat atcaataaaa
      361 aactgcttaa cagctatttc agctgttaag cagttttagg atttttagtt catattcatt
      421 gtttcttata atacagtgtt caatacttct cttaaagcac ttgttgattt gctgaacatt
      481 gctttttctt cttcgttgat tgataattca acaacttcac gaacaccatt ttcgtcaacg
      541 attgatggca cacctgtaaa gatatctttt tcgtcgtatt caccagttaa ataagcagat
      601 acaggcaata cagcttgttc gttattcaag atagctttta cgatacgtgc agtactcata
      661 ccaataccat agtaagttgc ttttttacga tcaatgatct cataagcagc attacggact
      721 ttatcagcaa tcacatctaa ttcatcttgg gcaatacgat ggtctttttc tacaatttca
      781 aacactggtt taccaccgac agttgtatgt gaccatgcag caacttctga atcaccatgt
      841 tcacctaaga tatagccgtg gacgctgcgt gggtcaactt ttaatttcaa tgcgatttct
      901 ttacggaaac gagttgtatc caatgttgtt ccagtaccga taacacgtga tgttggcaga
      961 ccagattcgt tccaagcgat atatgtcaaa atatctactg ggtttgaagc aacaacgata
     1021 ataccgtcga atcctgatcc catgatttct tttacgattt ggcgcatgat tgacgcattt
     1081 gttttaacta aatctaaacg agattgacca ggtttttggt tgataccagc tgtgataaca
     1141 acgatatcag catctttaca ttcttcatag ccacctgacc atacagccac attttcatcg
     1201 ccccaagcca taccatctaa aaggtccaat gcttcccctt ctgctttttc ttgattcaca
     1261 tcgatcaata ctaattcatt agagattcct tggttgatca ttgcataagc gatacttgtt
     1321 ccaacaaaac ctgtaccgac gattactact ttacgacttg tttttttcat gaataaacag
     1381 ctccttttca taactatttg ttactttcat gaaaagtata gtcttgttta ttcattttat
     1441 caacatgtta attataatag ttgttattat aatttttgat ggaaacgaat tcctgaatac
     1501 gcataacaga taaatgacaa atgtttacac ctatatcccc tgataataca accaaaagtt
     1561 tagaactgaa aataaatcca aaaaaattcg ctgcaacgat ataataagac agcaatcgca
     1621 cctaaaacaa ttttccttca tatgctttag tgaaagattt tcttcttaat gtttatttct
     1681 ccattcattg ggagtacatg ttgaatactt cttgaataat ttgaataaag aagtcgtttg
     1741 tttatatccg atacgattag cgatttcttc caacgaaaaa ctcgtttctt gaatcagttc
     1801 caacgcaaag tcgaattttt tctttagaac cagttcttga aaggaagcat caaaatacat
     1861 tttcaattta ttactaagat agaccggatt gtagccaaaa tgagctgctg tttcttttaa
     1921 cgtacaagaa gtaaaatgct tatcgatata atctaatact ctaagcatct cattttctgc
     1981 gcatccatct gctgatgaaa agctttgttg ttctaataaa tgagtcaatt cgatcagtaa
     2041 tagtgatgta tagagattta acgaacggat cttatgactg tatgtatcaa aataaatcaa
     2101 cagcatattt tcaatcaaat gcgtcaaacg acttgttttt ccgccgcgaa aatgaatgaa
     2161 ttgtgtatgt tccccttttt cttttgatgc atttatcaaa aaagaagaaa gtagattatt
     2221 tgtgcttgcc aaatgattca atatctctgt agataaggtt tcttctttta ataagatgtt
     2281 gattaaaata tcctcttgcc ctgtgtattc gattctttga gtagcttctt tatcaatgat
     2341 caccacgtca ctttcttcca tctctatcct ttctccatta ataatttgta aacatgtacc
     2401 gctatacata taattgattt caacaaaatc atgtgtatgc tcgggaactt ccgcatatcg
     2461 acggtgttta ctgatatgaa tatttccctg ttgaaataga tcccctcggt ggatttttgg
     2521 cggtgtctga agatggtaag ctaagtttaa atcattgata ttcattcctt gttcacgctg
     2581 cagagtttcg attgattctt cgttacgcaa aaaaaatgtc taattcagct agttccaatt
     2641 gactccctcc tttttgtaag cggtttcttt cgcttattag ttcacgctgt actgctatta
     2701 tatgcaaagc tatttttggt ttcataaaga ctttctatgg ttgggacatg aacttcctgt
     2761 gattccgcta ttgagagaat aaccacttca tactatattt ttgataagcg cttacaaaaa
     2821 tgaaaggaac atgcttatga ataaaaagat gatgaaattt tctctgctgt cagtctctct
     2881 tattttgact tctgctggtg cagtcacgtc tgcaattccc agtatgtcag ctacttatgc
     2941 cacgcgttct ttatctgctg ttgaattatt aacgactgct ccctctttta tggtcactat
     3001 ttttgttttg attagtagtt ttattgctag gaaaatcgga caaaaacaaa ctgtgatcat
     3061 cggattagtg attgcatcga tttatggtat cctccccctc ttctcacaaa gttacagtat
     3121 tgtcttagct tcaagagctg gactaggtat tggctttgga ttgatcaatt ctttggcagt
     3181 cagcatgatt gctgcctttt atcacggaga tgaacgagcc tctatgattg gttttcaaag
     3241 tgcatttcaa ggatttggtg cagctttaat gacatttgtt gcaggacagc tagtacagtt
     3301 cggttggcaa tatacctttt tagtctacgc catcagctta cctattcttg tcttgtttgt
     3361 tttgtttgtt cctacacctg aaacaactga acttcaatca catggcacag ccccaacaag
     3421 acaagtaatc aaaacgaatc ctaaaatgat tagttatggt atttttctct tgctggtcat
     3481 cgtggtatat aatgctgtat cgattaaatt agccacagtc ctgacgactt attctctagg
     3541 aaacgaaact aatgcagcga atatcttaac aatcatgcaa ttggcaagta tggctgccgg
     3601 agtttgtttt ggctggcttc agacaaaagc acatcgctat atgcttacac tttctttact
     3661 actgatggga atcggctttg tattgattgc cactgtgata aacctgtatg tgattgggtt
     3721 tggtgcgatc ctaacaggaa tttctttttc gttattcatt ccttacttat tcaaccaagt
     3781 aagtatccaa gcgcccaagt acgcggagag tttcaatact tcaatcttat tggttggcgc
     3841 taatttaggt actttcattg caccatatgg tttgctcttt ctctcttttg cttctcttgg
     3901 gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg ctgttgatta gcgcgcttat
     3961 tagccttcta gtagtacaca ggacgaatag aaagacagtg ataagagtag atgaagaaat
     4021 aaaagaacaa ttgacaacaa aataaatttg atggaggtcg tgtaaaatga caaatgaaaa
     4081 acataaagat agatggcttt ggtatcctgg agactttgag atccgccatg gactgctaca
     4141 aaatttccaa cgggaagaac gaggttttga ctggccagcg tactggtaca tggatgattg
     4201 ccatcggaac gtcaagttta agagatatta ctttcttgat caaccttcca tgtttaaagt
     4261 gacgatccaa ggagttgggt atgtagaaat taatggacaa aaacatccat gtggaaaatg
     4321 gctgacttgt ccagctggta aagcaaaaat tcgtatcttt gtaggacata catcagggct
     4381 accagcgatg tttatcgagg gagacgaagt aaaatcagat attggctgga cagcaagtaa
     4441 ttttattgaa gaatatccag ctggctggag tccgctttat gtcgatatcg caaaagatcc
     4501 aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc actgaaacgg agattggcgg
     4561 aggaatctta ttcgattttg ggcgagcagt gaatggctgt gtagaactaa tatctctgga
     4621 tgaccatatc gaatctatca ctctatgtta tggagagtcc gatgtagaag ctatggatat
     4681 cgattattgc tactataagc aaataggaat gacaagaaaa atgaaagctc gtaaacgcgc
     4741 ttttcgctat ttgtttatac cagaggtttc tactggaatg gttcaacttc aagtttttca
     4801 tgaattttta tctctttcga aacaagctgc tttttcaagc gattcttctt tactgaatca
     4861 gatctggtca gtatcagaag aaaccttcaa gttatgtagt gatttgtttt ttatcgacgg
     4921 tatcaaacga gatcgctgga tttggtctgg agatgcgtat caatcattca tgatcaatca
     4981 atatcttttt tttgacgagg aaatcaataa acgaacgata ttagcattaa gaggacaaaa
     5041 tgaaatcaag cagcatatga atactatcgt tgactattcg atcttgtggt tgattggtat
     5101 cgaaaaccac tatatgatga cgaaagacta tgaattttta cgcatcgtct accctaagat
     5161 gcaatcatta atgagatact tgatggagca aacgaatgaa ttagggttta tttatgggag
     5221 agcaaaagat tggatattta ttgattggtc tgaaatggat aaagaaggaa cgcttgctgc
     5281 agaacaagtc ttattactga aagcctatca ttccatgatc acttgcggag atatcttagc
     5341 tgaaacatat ggagagaacg aaaattatca tactcatctc tatcaagaaa aatatcaact
     5401 attgaaacaa aacctatttg actacttttg ggatgaagaa cttggcgctt ttatcgattg
     5461 ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat attttcgctg ttttattcga
     5521 ttttgctaac aaagaccaac agcaatcgat cttaaagaat gtcttattga atgacactat
     5581 cacacagatt actactccat atttcaaatt ctatgaacaa gatgctttat gcaaactcgg
     5641 acagacgaat atcgtctacc aaacgattct tgattattgg ggagggatgt tatctcatga
     5701 tgcggtaact ttctgggaag aatacgaccc tactcaaaca ggaaatgaaa agtacagtat
     5761 gtatggagat ccttatggaa aaagcttatg ccatgcatgg ggagcaagcc caatctatct
     5821 tttaggacgc tattttatcg gtttgtatcc aacaaagcca ggttatgaag aatttatgat
     5881 acaacctaat ctagattcat ttgaaacact gaattgtacg ctacctatca aagatggaaa
     5941 gatttcgctt gaaaaaacaa aagataagat cgtgattact tctgatagaa caggcggaac
     6001 agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa cagccacttg tcttaacaag
     6061 ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg acatatcaga agactttaaa
     6121 tcttcttctg atattgtcca agcaattttt attttgaagt ttgcttaatt ctgttcttta
     6181 gaaacaatgt ttgttctttt tccggcaaaa agaaagctag ataatacgac aagtaaaaag
     6241 aaagcgatga ttccaactgt caaggctatt tgaccgtatt ctcctagttt cccaagtaca
     6301 agtcctggta ctagataatc agtatcggca aatgtagaag aagcgatttt aagatctcct
     6361 aatactggaa ggataaataa tggcagccaa ctaatcaata agctattgaa agcagaacct
     6421 aataccgccc ctcttctacc gccagtcgag ttgccgaaga caccagaagc agctccgcag
     6481 aagaaatgtc ctactacccc aggaatgata actgtagttt gcaagcctat catagcaaac
     6541 atggatagtg ttcctacaaa gaagctaaca aaaaagccaa ttaatacagc attaggtgca
     6601 tatggaaaaa cgataggaac atctaatgct ggtttagaat cgggtacgag ttttttagca
     6661 atcccttgga atgctggaac gatttctgcc aacaccattc gaaccccttg aaggacgata
     6721 acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa agattacagc gttgtctcca
     6781 ttgcttagtt ctctttcaac aaatccagga ccagctaaca aagcaagtgc gacatatacg
     6841 atcgacatca agagcatgat cgatacagta gaatctcgca aaaagctgaa acttttagga
     6901 atctttatgt cctctgtaga acggtctttg tttccaaaaa actttccgat ccatccacta
     6961 gaagcataac cgatatttcc ggtatgccct aacgcaactt gattgtttcc agtgatgttc
     7021 ttcataaacg gttgacaaag agcaggcgta atagttaata gtgttccttc gaagaccccg
     7081 cctaataaga tcatccacaa atttgcagta aaccccgcgc ttactaaaat gactgctgtt
     7141 agacaagaaa cataaagcat tgcttgacct gtaagaaaaa tgaatttata tcttgtgaat
     7201 ctagcaagta aaatattcac gatcatccct actagcatga tcaaagcagt aggtgtaccg
     7261 tattgttgca aagcaatcgc cactactgct tcattgctag gaactactcc ttgagtatgc
     7321 agagctgttt ggaacatcga agcaaaaggg gttagtgaat tactgattaa accagcaccg
     7381 ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc cttggatcac ttctgaaaat
     7441 ttcttcttct gaagaacgag accaagcagt gcaatcaaag aaacgaggat ggagggctga
     7501 ctgacgattg acacaaacaa attcaacaat ttatccattg atagttcctc ccttgaatca
     7561 tttttattct tttgacaaga tcttcgttac tttttctttt acttcttcgc tgtcaatgat
     7621 cgattgcaaa ggtacgagct tttcatcagg tatagaagtt agcgtatctt tcaaagaatg
     7681 ctcaatgaag aaataatcag ccatatcgct agatacactt ccaacatccg tatgacttac
     7741 ttcaaactcg tccacatctg ctccaagatc tgcaagtgct tgtttgatgt tcatctctac
     7801 cataaaactt gttcctaatc cactttgaca tactgcaaaa attttcatat cattcgtctc
     7861 cttttcttat gatcgctaat atttcttctt ttgttgatgc gtgtaataaa tcttgaagat
     7921 tttgtttatt agaaagtagc tttgttaaat ttgccaaagc ccgaagatgt gcctcattat
     7981 cgatagcagc taaacagatg aacaaactaa tgggatgggc tggattatca agcagatcca
     8041 caggttgttg aagtttcagc aaagacatcc ctacttcgtt tacgccatct tctggacgag
     8101 catgaggtaa tgcgatgtga tcgccaatat ggataaacgc accatactgc tccacgcgct
     8161 cgatcatagc tttgatataa ctttcttcta ttttattttc tgataatagc ggctgtgcgg
     8221 caaatgtgat tgcttcttgc cagttttttg gttgatccga taattggatc atttcttctg
     8281 ttaataattc agatagcata ggacgttgat cctccttctt ttctaaaagt ttgttcattt
     8341 tacgatttaa tacttgatag agcttttctt cgtctacgcc ttcttttaat tcgatataag
     8401 gcagtagcgc cgacaacaat tcttgggcag aaggcattga aaatcctggt atcagccaat
     8461 ctctttgtac ttgattgatc agctgatttt tttccaagtc agtcatgatt ggatgcagaa
     8521 catagagaga ctttttctta tcagtaagtg tgagaggtac agtagaaaaa ataatatcgt
     8581 aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt agtctctgta aaaaagatca
     8641 tcgggaataa ttgctgaagt tcacttttta agatcaatga agagctaatg ccacttggac
     8701 agacgatcac ggctcttatt tttctatttc tttcttctgc gtccgctttt ctgatctctc
     8761 cgccaaatag tatagtgaaa aaaccaatct cttcttcagg gatgctcttc cctattcgtc
     8821 tttctaacgg aagtaatgct tttcttgtca tttcgtagat ggaagcatat tgatgcttaa
     8881 tttgatcgat catcacatta ggtaaataaa aagaatagga aatacgaaaa taggcaggga
     8941 ccaagtgata aaagacattc atcaataatt cacgaaacga agcaaattct acggccatca
     9001 ggaattccat gcgatggatg acttctgaag aacattccag caaaaaatca agtgcaggct
     9061 cctggacatc tccttcaacg atcgatgcta agcaaagaga gaaaaatata atctccgaat
     9121 tttgatgttc ggaatagcca aggccagtca atacttgtcg actaatgtca gtgactgcaa
     9181 gagaagccaa cagttcagca tcttttacca gttccatgga tgtttttcca attcgaatgg
     9241 ttgtcaaact caaataataa gctaattcat ccattcgatt tggtgcgaat cgaagatctg
     9301 atagtgaaat cgttttatct agcaaatctc taactttggc gtaataatca atggaaaggg
     9361 ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga ctgcatcaaa cgagcagtgc
     9421 gattttttgc ttctcttctt aatattgctt ctgaccctag cagcctaaaa ccagttttcc
     9481 tagtatacga cagctgacaa tcttccactt ctaattcatt tcttatcttt ttgatatctg
     9541 acaggactgt ccctttagaa acctgaagaa aatcttgaaa atgaaaaaca gaaacagacg
     9601 ttgcagtcga ataaaccatc aaataaagaa gatctcttcg ctcttgttcg gtatacacag
     9661 cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc gattgctcgg aatttcttta
     9721 tacggatacg ggtatcatcc acatattcgg caaaaggagt taattgtttg atcgcctcgc
     9781 ttgtatttac ttcgatgatc tctgctgctt tttctagact gatttctctt ggtgttttca
     9841 ggagataact aattttgtag ttcacagcgc tgttcacctc ctacttatat tctagtatgg
     9901 tagcgctttt aaatcgaggg gtgtttggta caacttacag ttggattttt tttacgactg
     9961 taccgaacga tttctatcga atggatacag aaaaaaagca ggtgaccaaa tgtctcctgc
    10021 ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt tattttttga ggtcagttct
    10081 tcaaattttt ctttgacatc ttcaaccgct ttttcgatat ctttttcaaa gtgattgggc
    10141 tcattaggct ttgtttcctc ttctacagaa tttgaaccag ataaagctgt ttctttattt
    10201 tctgtttcac cagagtttgt gccagctaat gattcttctt tgtcttctgt ttcaccagaa
    10261 ttagttcctg ctaaagccac ttcttcttct gttttagggg ccattgaacc tcttaatcgt
    10321 ttgtgttttt caccgtcata atttgtcatg ttggttcctc ctagtagttc tatacttttt
    10381 catcgtacaa taagacagtt tatgcgtcaa aaaacaaacc cgattcttac aaatatgtca
    10441 tatttttcct tctgattctt gaatgctttt gattccttct tccaaaattt tcatttgtaa
    10501 gattgtttct tcatctttga tgactttttc tgcgccatta actaatgtct cgtaaatccc
    10561 ttcgtagaca cggctgtaat caccaacctc ggaaattact ttttcttcat gataactccc
    10621 ttgttcatct atataggtca atacaccata atgttcgggc aaatcgatac caaagtcttc
    10681 atgatcaggc atgtaaaaca ttttcagatg ttcttcttgt cgatcttttg tttgtttcac
    10741 gaacatccct ttttttccgt aaacgacgaa acttggacgt tctttgatgc ggaaataact
    10801 ggattttatc gaaattttca tatttccata gaaaaaatcc agatcgaagt aatcattcat
    10861 tcgtcctttc cctaaaagtt gacgtacatc atagctgata tcatctggtt cgccgaaata
    10921 agaaagaact tgatctactg tatgacaagc atgtccatat aaatagctgt tcgcagtaga
    10981 aaattcatgc gtattttctg gtacttccgg tctgaaataa tcataatgca tttctacttc
    11041 taaaatatcc cctaattttc cgctttcgat tactttttgg actgtcaaga agtcagaatc
    11101 gaaccgtctg ttttggtaac attggataaa tagacctttt tcttttgcta atgcaaataa
    11161 ctcttctgct tcctttgacg tttctgcaaa aggtttctct actagtacat tcttaccatg
    11221 ttcgagtgcc atttttccat attcataatg aaattgacta ggtgtagtga tgacaactaa
    11281 ttggatctca ggatcatccc aaagatcaga cagctgatca gtatattcga tgtcttcgaa
    11341 tgttttccac actggttcag atggggaaaa aatttttttg atattgattt tgttcgtaag
    11401 ttttcttgaa aaaggcaaat gatagcgatt tgtactcttt ccattgccaa gataagcgat
    11461 tgtaagcatg agtggtgcct ccttttattg atgttttcag tatatcctag aaaagcgcag
    11521 aaagtattta taatagctat tctggtaaaa aagttcagaa aaacacttgt tatcattcgg
    11581 tttggtgaaa ttaatgttca aagtaaggat aaaaatcaaa aagatagtag cggaaccaag
    11641 tgtttatcag taagatagaa tttggaggtc agatcatgct tattgaagaa aaactaacaa
    11701 aacaagagtt gtttaccacg acagagaaac ggattgtcga ttacatccgt aggaatatcg
    11761 aagctgcagt ctacatgacg attgaagaat tagcaaaagc tacgtatact tcccattcag
    11821 ctatcattcg tttatgcaaa aaaatgggat tcagcggatt taaagaattc cgtttcgagc
    11881 tggggcggga agttcatcaa ttgattgctc agttcaatca aacgattgat cctaattttc
    11941 ctttttcaag tgaagatagc actcagcaaa ttgcaaagaa aatggcggat ttatcgatcc
    12001 agtcaataaa aaaagcacaa ttgcagattg aacatgaaga tttagatcaa atcggtaaag
    12061 tattgaacaa ggcacagcga atctttcttt ttgcaaaagg tgattctcaa atcacggcaa
    12121 ggaaattcca gaataaaatg gtcaagttga ataagttttt gatcatggct gaagaataca
    12181 gtgattcttc ttggaatgca gccaatttga ctagcgagga ttgcgccatt tttattagct
    12241 acagcggtcg catccatcat tatgaacgaa taatgaccta tttgaagcat gtagaggccc
    12301 ctaccttgct gattaccgga aatcagcatt cagaaatggc caaacaggct gatatgtgtc
    12361 ttgttatatc gcaggaggaa tatgattttg ctaaagtagc gactttctcc tctcaaattg
    12421 ccttcgatta cgtattaaat acgttattct cagtgatata ttctcaaaat tttgaagaga
    12481 atatccgcaa cttgaaaaac aaacaagaat tgatgcaaaa aggccttctt aaagaatatc
    12541 ggaaaactta agaaaagatc ctcatttagt aataagaaag atagtgtttg ataggctctc
    12601 tttgatatca tcataaaaac aggctgaagt tttctttttc aaacctcagc ctgtttgttt
    12661 acggaacgat tcgaatagat atattataga aatgaggaat agctaacttc tgtttcttat
    12721 tttgaacgat tgacgaaaaa gatatataag aaggaggaaa atcagtatac atgcagctaa
    12781 caatccatac aggaaaaatc gtttgttctc ggatactttc tgtacttctt tttgaacttc
    12841 ttttgcatga taaggaaccc ggtgccctct gacaagtaag cgatgactgt tgaccatata
    12901 aggtgtacaa gttaggagtg tcacgaaatc ctgccctttt tcgattctca gatcttttgt
    12961 atcagtcggt tctaccgtct tgatctgatc gacttgataa gcgagtgtcc gtccattgat
    13021 ttcgatatag aaagaatcac ctaaagtcag tttcggtaag tctgtgaaca actttgcttg
    13081 aggcagtcct cgatggctgg aaataacggc atgtgtattt tctccccccg taggataaga
    13141 cgttccttcc aataatgagg cacctttttc tagcagcaca gtattcgttt catcaaaaat
    13201 tggcaaatgg acattgatct cagggatcgt caaaataccg atcgtatgtt tctcaaaata
    13261 ggttctatcc tttttcgcag gttctttttt cttagtaaag ggatctgcac ctggatttcc
    13321 accttctttt gctaattgct ggtttttctt tgtcatgttt tcttggattt tagccatgac
    13381 ttcttcattt tctttgaccg cttgttgctg ataatgagag atgatctgct gatccagata
    13441 attattcagc gcgtcgctaa caaatggata agctagtatc cctattcctg agagaagcaa
    13501 gaaaagcatc agcagatcta gaagtttcct tttctttggt ttcattcgtg ttctccttgt
    13561 gatgactgtt tgataataaa agaaaaagga gcatttgcgt aatccgctgc tcctttttcc
    13621 aattcttgca aaaagactaa atctgactgt gcttgcgtct agcaaagtac agtccagcaa
    13681 tcagtagaag gactactcct gcaccgatat acacatagat tcccttaccg cctgttgaag
    13741 gaagtgtacc tttgtgttta tttggtattt tttcaggaga aatcaactgt cctgctgtta
    13801 cataagattg ttcatcgata gtaaatgcta cacggtttgt taatggaaca tatttttctg
    13861 gcgctttcgt ttcttccaga tagtacgtgc catattttag acctgtcaca tcgattaaac
    13921 cgttacttgt ggttgtaaaa accgttgctg attctttcgc cgatacccag ctgacggctt
    13981 ttgtggatgg gtcgatcgat aaatatttcg ctgtgtcact atcttgatca cgaacgacga
    14041 attctgctcc agcaagtgtt tgatctgatg taacgtcacc atctacttta acaaatcgtt
    14101 tgcccccagt aacgacatcg actgacggtg gtgtttgatc atttgtatgc ccgttatcga
    14161 catttgcttg gttagaaaat cctttggttg gatctgcttt ttcgttcaaa tgcatatagt
    14221 aaacaaattt cagcgtacca ccaggagtga gtgaaggaat ataattcgag tcaactgaaa
    14281 ccgtgaatcc gtttgtttgt tcagtgacag aataatttgt tggatcgatt tctttatccc
    14341 catcatacaa gacatacgca tacgttcctg aagtttcatg atcaaatgtc aaagcggcgt
    14401 catgagtatc aatgagtttg aatgtcgtgt acttgttttg tgttccttct ttatcagcga
    14461 tacctaaagg aatattgaca gagatctcat attggatttt ttctccgatt gcgacatctt
    14521 ttccattcaa ttgaggtgtt gttttatcaa ctttggacgt atcatttttg attgtctttt
    14581 ctacaggagt ttgactattt gccaagatct caaacggcgt gtttgtttga ttatcaatca
    14641 tttccgcatt atcaggagct tttacttctt ctaaattata ttttccaacc tctaaatgat
    14701 tgacgatcaa ctgacctttt tcaatcgttg cctcggcaaa gtcattgttg ccgatgtcat
    14761 aagaatgacc agtaatgaaa tgtttggctt tggtttgatc cgttgtccaa gtgtacaatc
    14821 catctgtgac actttggatg tattttttga cgcttggtct tccttcttct ttagaaataa
    14881 taaattctgc tccatttagt gcttcgtttt cggcagtacc gatttttgta actttcaacg
    14941 ttccatcatt accgactgta tttttagggt agagatggat agtatctagt tcttctgtcc
    15001 cgtatttata agagccatct gcttgtttga tcatctcata tacagggaaa gctaaaacca
    15061 tgtttgcggc agctgacact ccgtcttttg gttcttcttt gatcgtgtag actgcatctt
    15121 tcccattttg ttttttaggt aaagataaag tgacatttcc atcagcatct gtcgttcctg
    15181 aagcaactgg tgtaccagga gtcaaagact ggactgcttg ttttgctgca tccacggacg
    15241 ctcctttatc tcgttgcgca taaaattctt gagtgacgtt ataaactgaa aatgaaatat
    15301 cggctaatcc ttggtattga tcgaattcgc tcatttcttt cccgctgttt tggattaaag
    15361 gatcgggtaa atcagtcatt ttctttttat gaaggatgac ttgagctgct gtctcctctg
    15421 caaacacttt ttttgcgccg aagctgtttg tgagtaatgg taaaataagg aaaaggactc
    15481 ctaacataac gtttattttt ttatgatttt tcattttttc actcctgttc tttttttgat
    15541 aaacaaccag attgcaaagc ttgcgaatac cataccaaca acgcctaaaa gtgtattttt
    15601 cgtttcgttt gtttttggaa gaaatggacg gctattttct tttttattga ctactttcaa
    15661 tatactgtcc gcctctttat cctcagaaag aaaagttgaa atggtaaacg gaatttctgt
    15721 ttcacttaaa atataaccag aaggtgcttc gatctctttt aatcgataac ttcctgtttt
    15781 caacccggaa attgaaaaag cgccattttt atcggaaatc aggtgaagcg catctgattc
    15841 gttctttgtc cacttgtatc cgttggctgt ttgctggagg taatttcctg ctttgttttt
    15901 aataaggaat actgcgccag ctaatcgttt atctggttta tttgtctcga ttttgacaaa
    15961 ttgcttactc cctgtatata cagagacttt ctttgtactg ataccaaaac caaaatccag
    16021 tgttccgttg ttgttgattt ccttgttcgc ctgtgctgta ctgcttagct gcatctgata
    16081 agaaatggtc agtttttttc cagcatattt ttccaacgaa gggatcgaaa ataaaacatc
    16141 aaatccgtga ggttttttgt gtatgacaaa gccttctgtc agcttttctc cattcgatga
    16201 aatcgttaaa ctattaggta aaaacgttaa tgcttcatcc gcactatctg acaattcgaa
    16261 cttttgatag tcaaggatat ttacaggaat tgtcgtatgc aaggaataag tgattttttc
    16321 accaatcgta aaatcatttc taggctcttc taataccttc tcaaaaggtg gatcagggta
    16381 gtcgttttct tcatttttag gatacaaatg gatagttgaa agtttttgat tgttttgtcc
    16441 atgtacagga agaacaacta ccatattctc tgccttttct ttgacgactt ctggtgcttt
    16501 ggattcaatg aaaagatacg ctttatctct tttttcttga tctttagcgg ccagagaaaa
    16561 agaagcgata ccatcttcgt tgttaagagt tgtagttgtt gcttctgcgg taaacgtacc
    16621 ggaagacgca ccgttttttg cgatctctgt ttgtgcttct tctaccgttt tgcccttttc
    16681 ccgtagatgg taaaaagaat ctgtgacatc ataaacttgg aatgtgacac catttaatcc
    16741 tcgatacgtt tgtaataacg ctttttcttg tccgtcattt ggatgatttt tcggcagttg
    16801 cccgtttggg aaaagcaatt tgtgtaaagt aatctgaacc atttctgttt ctgtttctgt
    16861 tgctgcctga gtaaaagctg gtttaaatag taacaagaag agacacatac taagccaacc
    16921 aagttttttc atatttagct gccccctctt tcttttttca aaaaaagata ccaaatagaa
    16981 aacgcacaac caatcatccc tactagatag attcctaaac ggccaattcc tcccgtttca
    17041 ggtaatggta cttttgcctg attcgtgatg tctaaagaaa tctggttgtt tttggctcct
    17101 ggtgacagaa cagattcatg atcttgtcca tccacttgaa ttgacccatc ttcgtgtata
    17161 actatagtaa ctggctcttt tagaccttgg tatccttctg gtgtaaaagt ttcggtcagc
    17221 gtataagttc caggttttaa attttcgaat agaaaggtat ctgtttcttg tccatctttt
    17281 ggcgattcta agtccatttc tggtccctgc agtctgaatt ttgctccttt taatgtctgt
    17341 ccgttgtctt cttttttgtg taccgttaga tcaaacgctt ttagtcgatt ttgatgagtg
    17401 agcgtccata cattatttgt catctcggta ttttttcccg tgtaaatgat ttttccatat
    17461 ttatcgattt ggaattcata atttcccggc gaagtatcat atccggcagg accagctgtt
    17521 tcaacgactc ggtatttacc aggtgtgagt gaatcaaaac tggcaagacc tttttcattc
    17581 gttgtttggc tgtcaattgg ctgataagta ccatttgctt cttttttctg tagggaaaat
    17641 gctgctcctt taagatttat ctctttgtcc gtcccttgca tcgcatactt acgtactgct
    17701 acaggaactt caacaaaagg attttcaata gtcaactgga tcgtatcatc ggaagtagtg
    17761 actgtttttc catcaatggt taccgtacca tcagaagcga tcttgatttc ccaagtagtc
    17821 tttttgctta atccatgccc ttctggagct tttgtttctg tcagcgtata ggtcatttct
    17881 ttttgcaatt tgacattttc tggaagagaa taggttccgt cgccatgatc tgttagtaaa
    17941 gtatcaatag aatctcctgt taatttgaag acagcaccga tgagattctt ttcacctgta
    18001 gaggaatttt tcgttatttt caagtttaac ggtgtgaatt gtttagtatt tttccatgtc
    18061 attgcatcta tttgttgaga atcgtatcct ggtacaggta attctttgat tgtttggtaa
    18121 ctgaatgctt gaccttgatt attgtattga ggtagtgata atatctcttg ataactttct
    18181 ccgctatttg cagataattt gtcaatgtct gcacgttccc acgaatttgt agtatcttta
    18241 gctggtttaa tgattcgaat atatccgttt ttccaagctg cagcatttgt cgtatgttcc
    18301 cgttgaatct caaaagtaac ttgatctgga cgatcagcta gattgttgtc aaattcttcc
    18361 cataactttt tgatgtgaag actgactccg ggagctttag cagatggtat accgaattca
    18421 gctaattcat tggtatcaat gtttggctgg aaagttgtcc tgccgttcat ttgataccag
    18481 aaatttggat ggaagtcctc attttctgtt tggattctca cttggtaatg gatttggatt
    18541 tcttggtctt ttcctaaata gatctgattg ctcttgatgg tatttccttc tatggaaata
    18601 gatggagtga ccgttgtagg acttgtcccc acactcttga ctgaaagtgt accaggctga
    18661 taaatgaatg gttctgcgat tggatcattt atttgtccat tgcttacagt tgctgagatc
    18721 tgtacagctt ttttggctag gtattcagag atatctgttg catgatcagc tgattcatag
    18781 taaagatcgc ctttttcatc tgatgaaacc atttgtcgca tacgagactc tacttctgct
    18841 tttgagagac cagctgccgg atcgctttga agttggatgc caagaccatg tatttcgatt
    18901 cctcgttctt tgagtgccat cgcttctccg atggttgcga taaacgtact gtcgattctt
    18961 ctggatagat tgttttggtc aggtgcatca tagattcttg agattagaga agtatttccc
    19021 ggccgatctt gcgtattaga aaactgagtt ccgtaataat tgctgcttga ttgtgcgtgt
    19081 acccgctgta ctttatagga aaatgttggt acaccatccg tcagcaaaac gatcactttt
    19141 ttatgtccat ttggaacgga tagcatgctt cctgcatctc ttagtgcttt ttgtgtaaaa
    19201 gtaccaccat ttgtccgtga aggtgtaatg gattttactt gatttttcac tgaatcaaat
    19261 gaacccatct gtactgcacc gttactgtag ctatatcctt cgcttgagta gccgacatat
    19321 cccatattga ttttgtctgt gataccgcta tctgctaaag tatcgacaaa acgatcgaca
    19381 ccaatcttta cttcaccgat ccgattattg tcgttcatac ttcctgacca gtctacgacc
    19441 aatacgagat caagaggcgt gatatctttt tgtacatttc cacgagcatt caaataaaca
    19501 tcaaacaatc cgggtgtact tgtttccttt gcatactttc gtatcgcata gtctgcttga
    19561 ttaccggttc ctccatattc gatatacgaa tgggtccgat cattaggatc tccattccaa
    19621 gaatttatac catcccattg atttgttccg tttttattgc cttgatgatc aagaacattt
    19681 gtatttcctg taggttgcca actggcttta ggataagtgc cctgatcatc tgttgtatat
    19741 tctgtaggaa tcgtttgatt agatggcaca ctcacagctg gcaaggataa tgtaggcaat
    19801 gtaaatgggc tgacagaatt ttcgttttct tttgatgatt cttcttctat ttcgtctggc
    19861 tttggtactg acacatctgt atctttctta ttttctgtct catcaataat gtcatattct
    19921 ttattatcga cgatcagctt gtttgcctgg ctgtggtaaa gtgctggatc gattggaaaa
    19981 ctcagatcga tagtttgttt agtgtttgat gagatttcca acgtaatttt cccgctattt
    20041 acctgataag catagccatc tccttctttt ctttccacat cacttaagta attttcgact
    20101 agaccaattt ctttttctat cttttcagca ctggtgttat tcgcatttag cttgatgttc
    20161 agtgcttttt ttccagactg atccgaaatg acttcagctg aaattgtttc taaaactgtt
    20221 ggatcatctg tagtgtctgc tattgcagat aatggcacaa agtttgataa actcaatatt
    20281 atcagcatga aaataacttt cagtttcttc cctgtggtcg tcatttttcc cctccttttt
    20341 ctttttttct gttttgacgt gaatagtgaa aattattaaa ataaacaggc gttacataat
    20401 tttatttatt ttaatatgat aattattata cgaagggcgc taaagggaat cacaccaatt
    20461 ttcataccaa ttggtttcat ttatgaagtt gattccttcg attaatgagc gtttcattga
    20521 aagaaaaggg ttgagggaga aaaacatatg atcaggttgt tggaatgaaa ctatagaaag
    20581 atggaaaaaa tcattcgaat tctgtccatt tctgacaagt ggatgactaa aaaagaactt
    20641 gccaaacgaa ttggtagttc agaatctaca tttgtaaggt atatcgaaga gatcaaaatc
    20701 cgctggggaa cggatgtaac gattcaatct tcacaaaaat tggggtatcg tcttgaacat
    20761 ttgaatgtac cagcttattt aaatgttctg atggaaatgt cgcaaagttc tacaaacagt
    20821 cgtttattaa atgagattat tgtcaatccg ggtaaaacga ttgagtatta ttgtgaaaaa
    20881 atttccatca gccggtcttc ttttacacgt aagttgaaac aatgcaatca aattttagaa
    20941 agctatttat taaaagtgat cgttgaccaa ggttttcagt taactagcct taaaagtgag
    21001 ctccagttgc gtatctttgt tacctttttc tttttaagtt actacggaag acgcacgttg
    21061 ccatttccaa tcgaaaaaaa gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg
    21121 aatttgtttc aggaagaaag cccatatgag caatctttct ttattatgta ttttgttgtg
    21181 catctcatga gagaatccca aggaactcca tttgaatgga aagctggaga ctcttatgta
    21241 cagttgataa atatcgataa aaaagacttc ttaatcttga aaaatctctt aggaaaattg
    21301 aagtacagca gctatataaa agtagtgaac tattttgtca atacgatttt ttcagaattt
    21361 tcggagaaag aaaaaaatga aatcagtgag cagatacgaa aaaatatgtc ctactgttcg
    21421 ttttcatccg ctgattgcaa agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa
    21481 atttattttt tctcccgtat tttcccatac gatacgactc atttgacgat ccgtgcaact
    21541 tactttgcac agcagatgca acaccgccag atacgtctat atgctggatt tcgtgcagaa
    21601 cttcaatttt attccactct actgaatcag aatctgattt attatttatc tagctatatc
    21661 ttttggatgc ttatcggtaa tcctgaaatt caccattata cttctgataa aaaaatactg
    21721 attatcagcg acttaagtct tcgacatagt caatatattg aagaatatat cagtgatatt
    21781 cttgctgtgc ataaaattca ctctgagaca actgctatta ctgaagatca gctgtcgaaa
    21841 tacaatcttc tcgagtatga tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt
    21901 cctcatatat tgattgatga tagtgtgtca ttcgcaaatg aagaagaact catccgctta
    21961 ttcgagttat gaataatcaa aaaacagcta tgtataaacc accctgataa aaatttcagg
    22021 acaaatacac atagctgttt ctttttattg tattccttca gcaatccaac gagatttata
    22081 gcgaaagtct tttaacaact cttcttttgt ccaattattt gcaatgagat ccgtcaaagt
    22141 gttcattacg agggtcatgg aaaagtatag gaaactaact gcttcttccg acgattgaat
    22201 ggagaaatga ttgattctta ataattccag ccatttttcg ttcattggag aatcagccga
    22261 tagttctcta cgtttggttg tctgctgatc atttcgctgg gttaacaagt ataacctttg
    22321 ccaatagata ctttcgtgat ctaagtcgca gcaccactct aaatattttt cgatccccaa
    22381 aaagaaatgt tgtttgtttt cgctaatgaa ttttaagata tcttgatgga ctatgtgaga
    22441 actgcttttg atcgtataca aataagcatc ttccaaatct tgaaaatatt tataaaaagc
    22501 accgcgcgac atccccatct tgtcaacgat ttctgagact ttgacttggc tgatgtgctg
    22561 tgagcaaaat atctccagta ataggtcatc gattctctgt ttctttgcgt tcgataaatt
    22621 gaaaaaagtt tcttttggca tccacatctc tcctttatct ctgattatac gtggatagcc
    22681 cttcattgtc atgaaggatt cattccttct ttttctcttc ttctgtcaag tacccatctt
    22741 ccattcgata aacgcgatca ctccattgaa tcatacggac atcatgagta accataatag
    22801 tcgctttttg tttctcatga gcttctttaa ctagtaattt gacgacttca tatgcatgat
    22861 ctgtatccaa actagctgtt ggttcgtctg ctaagatcaa gcttggctca ttatataaag
    22921 cgcgagcaat tgccactctc tgtctttctc caccagaaag gtctttagga taactatttt
    22981 taagtgactg gatatctaaa gattgcagca aggaatcgat ttttgttgaa tcttcttttt
    23041 ttgaagtaac ttggtcaact agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat
    23101 tagagctttg gagaataaat cctatttctt taaagcgtaa atctgctcgt tttttttctg
    23161 gaagctcagt tagtgagtga ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc
    23221 ctcctgaaat ggtaaggaaa gtacttttac ctgaaccaga aggaccaatg atgctgacga
    23281 attctccttc ctttacataa aaatcaattg ctttcaaagc tgagaccttt gtatgaccag
    23341 aaccaaatgt tttggtaact tttttcatcg ttaatatttt tttcattttt attaacctcc
    23401 tattgctgta attgggtcta ctttggttat cgtacgtatc gaaaacaatc cgcctagcat
    23461 tgcgacaatg atcaaaatcc cactatatag caaccattgt gaccaaaaaa cagcaaaagg
    23521 catcgcgctt ggtaaaatca gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc
    23581 taccacccca acaataaagg actgtgctac taaagattta gcgatgaaga aatttttgat
    23641 cccttgtgct ttcattactc caaaaatagc agtcttctga agtgtcatca cgtacatgaa
    23701 aataccgacc acggcagcaa ccactaagaa caaaaagtat atcatcgcat ttaaggtcat
    23761 attttgggca gaatagccag gaatactttc aatgaatgtc ccaatagtta ataactgtag
    23821 acccttcgat gctttttctt tcgaaatttt agctgaatcc tttattacga tcccattgat
    23881 cggctgttct tcttcagaag caaacggctg ttgtccatac ttcaaagcag tccacgtatc
    23941 tagggacgca tagatgacgg gagaaactgt gtaataagtt tcaggaaaga taccgacaat
    24001 cgtcaaagga tcgtcgtaac tgccaatttt gatcgtatct ccaattttat agcctgaatc
    24061 agccagattt tgagagatga tgatttcatt ctttgtttta aattcatgcc ctttcgtgag
    24121 ttttggtaat aaaaatgctt ctttcgttgt gccgaaaact gtaacatttt ctttgttttt
    24181 tcctttgatt gctccgctgt acaatccgat tggtgctttc tctcctcctt caatgtattt
    24241 cagatctcct cttgttaatt gtgaagctgc aaatacttga tttgcatcct ccgacaaaac
    24301 aatctcttgt gcgtcccaat catcgattgc ttttttaaac tcttctgaaa gtccattcgc
    24361 tagtcctgac aacatgaata cgacatacgc aatcaagaac ataataccga cgatcaagcc
    24421 atatcgtaat ttagaatagc gaatttcttt gatagctaga aacatttgtt tttcctccct
    24481 atgatagatt gatagtgaca aaatgtcact tgttgaatag tgtaactcta aagtgacaat
    24541 ttgtcacttg tgttgttttt ttcttatcaa taaattgtct aagacatctt tcgataaaac
    24601 cttaacatga gtcataaaaa atttatttaa gccttattta tgctttccat gataaactaa
    24661 agtgaccaaa tcaatgaggt gggaaaatga ttaaactatt agtagtagaa gacgataaag
    24721 tattatccaa taatataaaa gaaatactcg ctgcaatcgg cgagataaca caagtttatg
    24781 atggtagtga aggactgttc gagttggaaa gtggagttta tgatcttgca atcttggatt
    24841 tgatgatgcc ggaaatgaat ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac
    24901 agactccagt attgattttg actgcaaaag atagtttgga agataagatt gaaggatttg
    24961 aacgaggtgc agatgattat ctaacaaaac ctttctatcg agaagaactg atcatgcgcg
    25021 tgaaagcatt attgaaacgc tcgctaggtc tctttgatga aaatgttctg aagtataagg
    25081 atatcagttg tgatttaaaa agcaatgaag tcttgtataa agaacaagtt ttgcccatac
    25141 aaggaaaaga gtttgaattg ctcgtttatt ttttacaaaa taagggaatc attttgacaa
    25201 aagagcagat atttgaccgg atctggggat ttgattctga tacgacgatt acagttattg
    25261 aagtttatat gagcaactta cgaaaacatc tgcgaagtac tggattggat aaagaaatca
    25321 aaaccttacg taatgtcggt tatatactgc aaagggtgtg atgcaatgaa tggagatatt
    25381 acacgaaagc aacgtagtcg cttttttgcg acaaatgtag cagcatttgc ttttatcttt
    25441 tttcttttgg gattcattac tttacagata ttaaaccaat ctgcatacag agaaacagat
    25501 ctgtcattag agaaaataaa ttttgattct cgaacagtac aattagagat acaaagatat
    25561 cagcaaggcg atcccttttt aaacccgact caaacggaac aaaacagaac aaacatggaa
    25621 cagccattaa tggaaccatc aaatgaccgt tttaacactc aagtgatctt gtggtctaaa
    25681 aatggtgaga tattgaataa agcagctatt ggtgggcgtt tcaaccaaat caaagaatta
    25741 aaaatggata cgaaaaatct gaataccatc caaaatattg aattaaatga agacagtggc
    25801 tcatctgccc tctctttccg ttcgattacg aaaaaagcgg agacgaatca agatggcgtt
    25861 gcctacatcc aaattttaga aaatacaaat caaatcaaga attcattagc gacttttcgc
    25921 acgattatga ttttgtgtat gattgttttt tggattctct ctattggtat cagttattac
    25981 ttgtctagtc taagcatgcg ccctattatt ctttcttgga gaagacaaaa agaatttgtt
    26041 gagaatgctt ctcatgaatt gcgcacaccg ttaacgatta ttcaaaatag tttggaacat
    26101 ctttttacca agcctaatca tactattatt gaagaatccg aaagcatcgc tcaagcattg
    26161 tcagagacga ggcgcctcac tggattgact tcggatttgc tgacaatcgc tcggaatgat
    26221 tctgatcagc agctgctttc aaagcaaatg atcaatacac aagaatatat cgaacaactt
    26281 gttaaaccat ttcaagagat ggccatcata gatggaaaag agtttattct ccataacgat
    26341 gcaacattag aagtaccaat tgatgaaaaa aaaatccatc aagttttggt tattttgctg
    26401 gataatgcat taaaatatac gaatccagaa gataagatca ttttggattc atctgttaac
    26461 cataaagaat ggctgattca cgtaaagaat actggccctt ctatcagtga agaagatcaa
    26521 gcaaggattt ttgatcgatt ttatcgggaa gatcaatctc gggcgaaaga gactggcgga
    26581 tacgggcttg gcttggcaat tgcaaaacag gtaatcgaac agcatcatgg acagatttct
    26641 gtatccaatt atcttccaca aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa
    26701 aaataatgct tatccctaaa aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt
    26761 tgcgaataaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttctttaagc
    26821 tgaccacttc tgtcacaacc tctttagctt acgcttacga ttttttattg cggtcttcaa
    26881 tgtgtacatc accgcttgtg tcaatatcca attcttctcg gctaacatct tctgtcactt
    26941 tttcaacatc ttcatgttct tctttatgaa tcccaacttc ttcacgaata actgtatgtt
    27001 tgcttacatc tacttgctct tctttgattg ggatagtaat cgtgtcatct tccaattttt
    27061 cgtctgttcc ctgtgaagat tgatctgtta ctggtttgcg ttcgatgacg atttcttcac
    27121 gtttgaccgg tacttctacc gtttcagtat cgttgaccac atgtttgtgg atgtcgactt
    27181 ctccagttgt cacatcatgt gtatttacat ctaatttttc ttcttttagt tgaattgtat
    27241 catcatctag atttggtttg cgatcgatgt cttcagtctt gcttgtaccc attgtatcag
    27301 tctcgccagg tacgtccatc ggatcagttc ctggtgtcat attcaatgga tccgttgtat
    27361 tttgaaagcc tgtcgtgcca cctacgtcca ttggatctgt tccagtgcca gtttctaatc
    27421 cagtccccat tgtgcttgct gggctaacag tatcttgtgt atccattgta tcttctgttg
    27481 tttctggctg acgatagcct tttactgtga tgacaaattt tccatcagaa atatctcctc
    27541 tatattgata taaaggatca ttttctggtg tagccgtctc agaatcatag gtatcaaaag
    27601 aaaaagtatc ttttattttt tcccataaag aacggtcttc ttcaccatcc atccgatttt
    27661 catctgtttc aacatctata ccagacaatc cgtcaaatcc tagtctagat gctgcttcat
    27721 cagtagtata aataacgata tcatctcgtt catacccttc gttacgcaac cgttcaatta
    27781 ctgataatgc ttcttccctc gtactatagc ttccgatgac cgttaaattt ttctcttcca
    27841 tgtaaattcc tcctagcaaa tagatttatg attggtatat tgttacttat aaatttattc
    27901 tataggagac gattg
//
LOCUS       DADXTH010000031        27633 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.031, whole genome shotgun sequence.
ACCESSION   DADXTH010000031 DADXTH010000000
VERSION     DADXTH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.031"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="IWI97_001328"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="IWI97_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBC2462839.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IWI97_001329"
     CDS             complement(2020..2922)
                     /locus_tag="IWI97_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2462840.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="IWI97_001330"
     CDS             complement(2919..3611)
                     /locus_tag="IWI97_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBC2462841.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IWI97_001331"
     CDS             complement(3654..4295)
                     /locus_tag="IWI97_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2462842.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="IWI97_001332"
     CDS             complement(4333..4647)
                     /locus_tag="IWI97_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462843.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="IWI97_001333"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="IWI97_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="IWI97_001334"
     CDS             complement(5984..6916)
                     /locus_tag="IWI97_001334"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBC2462844.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IWI97_001335"
     CDS             complement(6913..7689)
                     /locus_tag="IWI97_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBC2462845.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IWI97_001336"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IWI97_001336"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBC2462846.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IWI97_001337"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IWI97_001337"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBC2462847.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IWI97_001338"
     CDS             complement(12014..13294)
                     /locus_tag="IWI97_001338"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBC2462848.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IWI97_001339"
     CDS             complement(13313..14239)
                     /locus_tag="IWI97_001339"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBC2462849.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IWI97_001340"
     CDS             complement(14252..15535)
                     /locus_tag="IWI97_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBC2462850.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IWI97_001341"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IWI97_001341"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBC2462851.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IWI97_001342"
     CDS             complement(16323..17234)
                     /locus_tag="IWI97_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBC2462852.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IWI97_001343"
     CDS             complement(17231..17695)
                     /locus_tag="IWI97_001343"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBC2462853.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IWI97_001344"
     CDS             complement(17700..18398)
                     /locus_tag="IWI97_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBC2462854.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IWI97_001345"
     CDS             complement(18385..18771)
                     /locus_tag="IWI97_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462855.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IWI97_001346"
     CDS             complement(18918..20588)
                     /locus_tag="IWI97_001346"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBC2462856.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IWI97_001347"
     CDS             20771..21004
                     /locus_tag="IWI97_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBC2462857.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IWI97_001348"
     CDS             complement(21103..21534)
                     /locus_tag="IWI97_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBC2462858.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IWI97_001349"
     CDS             21613..22026
                     /locus_tag="IWI97_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBC2462859.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="IWI97_001350"
     CDS             complement(22064..23212)
                     /locus_tag="IWI97_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBC2462860.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="IWI97_001351"
     CDS             complement(23334..23930)
                     /locus_tag="IWI97_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2462861.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IWI97_001352"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IWI97_001352"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBC2462862.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKNGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEMAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="IWI97_001353"
     CDS             27160..27591
                     /locus_tag="IWI97_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2462863.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
BASE COUNT         7799 a         6089 c         4879 g         8866 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatta agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctctttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagtgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc atctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac cattttttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gtt
//
LOCUS       DADXTH010000032        27553 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.032, whole genome shotgun sequence.
ACCESSION   DADXTH010000032 DADXTH010000000
VERSION     DADXTH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.032"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(203..1006)
                     /locus_tag="IWI97_001354"
     CDS             complement(203..1006)
                     /locus_tag="IWI97_001354"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBC2462864.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1026..1868)
                     /gene="kduI"
                     /locus_tag="IWI97_001355"
     CDS             complement(1026..1868)
                     /gene="kduI"
                     /locus_tag="IWI97_001355"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBC2462865.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(1884..2891)
                     /locus_tag="IWI97_001356"
     CDS             complement(1884..2891)
                     /locus_tag="IWI97_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBC2462866.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(2898..3542)
                     /locus_tag="IWI97_001357"
     CDS             complement(2898..3542)
                     /locus_tag="IWI97_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBC2462867.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(3642..4400)
                     /locus_tag="IWI97_001358"
     CDS             complement(3642..4400)
                     /locus_tag="IWI97_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBC2462868.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(4527..5678)
                     /gene="fucO"
                     /locus_tag="IWI97_001359"
     CDS             complement(4527..5678)
                     /gene="fucO"
                     /locus_tag="IWI97_001359"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBC2462869.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(5760..6071)
                     /gene="rhaM"
                     /locus_tag="IWI97_001360"
     CDS             complement(5760..6071)
                     /gene="rhaM"
                     /locus_tag="IWI97_001360"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBC2462870.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(6073..6918)
                     /gene="rhaD"
                     /locus_tag="IWI97_001361"
     CDS             complement(6073..6918)
                     /gene="rhaD"
                     /locus_tag="IWI97_001361"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBC2462871.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(6932..8188)
                     /gene="rhaA"
                     /locus_tag="IWI97_001362"
     CDS             complement(6932..8188)
                     /gene="rhaA"
                     /locus_tag="IWI97_001362"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBC2462872.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(8185..9645)
                     /gene="rhaB"
                     /locus_tag="IWI97_001363"
     CDS             complement(8185..9645)
                     /gene="rhaB"
                     /locus_tag="IWI97_001363"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBC2462873.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            9798..10667
                     /locus_tag="IWI97_001364"
     CDS             9798..10667
                     /locus_tag="IWI97_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBC2462874.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(10793..11692)
                     /locus_tag="IWI97_001365"
     CDS             complement(10793..11692)
                     /locus_tag="IWI97_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462875.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(11857..12681)
                     /locus_tag="IWI97_001366"
     CDS             complement(11857..12681)
                     /locus_tag="IWI97_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBC2462876.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(12692..13987)
                     /locus_tag="IWI97_001367"
     CDS             complement(12692..13987)
                     /locus_tag="IWI97_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBC2462877.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(14003..14482)
                     /locus_tag="IWI97_001368"
     CDS             complement(14003..14482)
                     /locus_tag="IWI97_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBC2462878.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(14488..15516)
                     /locus_tag="IWI97_001369"
     CDS             complement(14488..15516)
                     /locus_tag="IWI97_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBC2462879.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(15533..16639)
                     /locus_tag="IWI97_001370"
     CDS             complement(15533..16639)
                     /locus_tag="IWI97_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBC2462880.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(16868..19465)
                     /gene="adhE"
                     /locus_tag="IWI97_001371"
                     /gene_synonym="adhC"
     CDS             complement(16868..19465)
                     /gene="adhE"
                     /locus_tag="IWI97_001371"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBC2462881.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(19770..20150)
                     /gene="yajC"
                     /locus_tag="IWI97_001372"
     CDS             complement(19770..20150)
                     /gene="yajC"
                     /locus_tag="IWI97_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBC2462882.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(20247..21392)
                     /gene="tgt"
                     /locus_tag="IWI97_001373"
     CDS             complement(20247..21392)
                     /gene="tgt"
                     /locus_tag="IWI97_001373"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBC2462883.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21610..22308
                     /locus_tag="IWI97_001374"
     CDS             21610..22308
                     /locus_tag="IWI97_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBC2462884.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(22476..25115)
                     /locus_tag="IWI97_001375"
     CDS             complement(22476..25115)
                     /locus_tag="IWI97_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBC2462885.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(25734..26765)
                     /gene="queA"
                     /locus_tag="IWI97_001376"
     CDS             complement(25734..26765)
                     /gene="queA"
                     /locus_tag="IWI97_001376"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBC2462886.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            26958..>27553
                     /locus_tag="IWI97_001377"
     CDS             26958..>27553
                     /locus_tag="IWI97_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2462887.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         7653 a         6131 c         4856 g         8913 t
ORIGIN      
        1 aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg
       61 aattccttct tatttcttgg agttaaatgc ttctgtccca gcctcttttt tcgaataaat
      121 gatgtttaat cagctgtcct tcggtattcg ttcaatccgc tgatttcaca acctctccac
      181 taaacagtgt actaaagctc ttttaaggtt gttttccgat ataggcaagt atgcctccgt
      241 ccacatacaa gacatggccg ttaacgaaat cagatgcgct agaagccaag aagacagatg
      301 gtccagccaa atcgattggc tcgccccagc gtgctgctgg cgtacgtcca acgataaatt
      361 gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg taatggagca gtttgcggtg
      421 tagcaatata acctggtcca attccattac attggatatt gtattgaccg tattcagaag
      481 cgatgttttt cgtcagcatt tttaatccgc cttttgccgc agcataagca ctgaccgttt
      541 cgcgtcctaa ttcactcatc atggagcaga tattgatgat tttaccgccg cctttttcga
      601 tcatatccgg aataactgct tttgaaacga tgaatggggc atttaaatcc acgtcgatta
      661 cttggcggaa gtctgccgca tccatttcga tcatcggtgt ccgtttgatg atccctgcat
      721 tgttgactag aatatcaact gaacctacat cttctttgat ctgttgaacc atcgcttgaa
      781 ccgcttcttc atcggtcaca tcgcagacat aaccttttgc atcgatgcct gcttctttat
      841 aatgcgctat accttcatca actgattctt gattcaagtt attaaatacg atttttgctc
      901 ctgctgtagc taaagagcgt gcaatctcaa atccaatacc atagacagca ccggtgacta
      961 atgctacttt tccatctaaa cgaaacatat ccatactaaa ttccatcatt ttctctccct
     1021 tatgcttatt ttaattgatc catttgcacc atatccatgt ctgtataggt gatattttct
     1081 ccgcacattg cccagatgaa tgagtaattg cttgtaccta cacctgaatg gatcgaccag
     1141 cttggcgaaa taatggcttg ttcattgtct acaactaaat gttttgtttc atctggtttg
     1201 cccatcatgt ggaacacacg tgtatcttca ttggcataat caaagtagac ataagcttcc
     1261 atacgacgtt catgtgtatg acaaggcatc gtattccaag cgcttcctgg ttctaaaatc
     1321 gtataaccca tttgtaattg acagctttcg catacatttg gatggatgta ttggtagatt
     1381 ttacgttcat tcagtgtcag gccttcacca gtctccattg gttttacttg gtcgatactg
     1441 atttttacat ttggatattt atgatgagct ggaacagaac taatatagaa tttagctggg
     1501 ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt cttttccgat atagtatccg
     1561 tcttgttttt tcatttcttc tttttgccca tcgatctcga tgaatcctgg tccaccgata
     1621 ttgatcacac ctagttcacg acgctctaag aagtaatcaa cacctagttc tttgtctaat
     1681 gtgatttcta attctttttc agtaggtgtc actccaccaa agatcattcg atcgttgtgc
     1741 gtataagtca aactgatttt ccctggttca aatacctttt ctactaaaaa ttcttttctc
     1801 aattgttccg ttgaataatg acggatatct tctggactgt gtgtataacg tgtttccatt
     1861 tcttgcattt tcttctactc cttctatcga atgatttttc cagagccgca tgctaaaaat
     1921 tgtgcgactt cttcttgact aaattggttg cagtccccat ggatcgtatg cttcaaggca
     1981 gaagctgccg tagcaaaatc aatgatttct tgcggagaca tattggataa aatcccatgg
     2041 agcacgccac ctgcaaaagc atctccgcca cctactcgat ccacgattgg ggtgattcta
     2101 tggacttggg attccacata ttcatggttc atccacaatg tccccttcaa ttcattttca
     2161 cttgctgaat gaacggtacg cttggtcgaa taaaacacct gaatattcgg gaacttttcc
     2221 tgcatttttt gataataata aattagaggt tgttcttctg cttctggcgc tttctcgata
     2281 cctaacaaat aaagagcatc catttttcca gctgagcaga tatctactaa aggtaaaagc
     2341 tgatggatta cttcgctggc ttcttgctgt gtccataatt ttccacgata gttgatatcg
     2401 aagctgatca ggcagccagc tttttttgca gcttctatca cctgtttcgt cattttttgc
     2461 cattttttag acaaagctgg ggtgattcct gaaacatgaa atatatctat tccttggaat
     2521 aactcctcta gcgaccattc caaatgatcc atttcagcaa aacttgatcc ggcacgatca
     2581 tagatcactg aggcggcgcg ttcgccacta ccagtttcca tataataagt acctagtctt
     2641 gatccaccag ataatacctg tttcgtatct actccataac attgcaagtg ctttttgact
     2701 gcttttccca atgcgttatc aggtactttt gtagcaaaaa tcgcttcatg tccgtaatta
     2761 gctaaagaaa ttgctacgtt tgcttcccct ccgccataat gggcagcgaa tcgttcactt
     2821 gaatcgatac gggttccctg ctctgtggac aatcgcaaca tgatttctcc gagtgtcact
     2881 acttttccca taagcagcta cctcttgatt tctttcattt ttgctgcata ctgttgcgcg
     2941 acttccgtta ctttgtcaaa atctcctgta gctgcgggtg ctaaaaggtt cccacctact
     3001 ccgactgtta cgacaccagc atcgaaccac tctttcatat tgtccagact cacaccgcca
     3061 gttggcatga tattgacttg aggcatcggc gccttgaagg cagaaatgat acttggtcca
     3121 taagcgcttc caggaaatag tttgacgata tctaccccgc ttttcagcgc tgttttgatc
     3181 tcggtaatag tcatacatcc tggtaagtaa ggaatttgat acagattgca catctctgcc
     3241 gtttcttgat caaaggaagg gctaacaata tactctgcgc cagctaagat agccaagcga
     3301 gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta tttctggttg gtctttgtag
     3361 aatgacaaaa gttctttgat tacttgatct gcttgaggga ctgtaaaagt caactcaata
     3421 ccagttagac cacctttgat gattgcgtgg caagcattca aagcttcttc tttgcttttt
     3481 ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag ataaaatatc aatttttttc
     3541 atcttctcca cctatttcct atttagaaat tttattttat aacttggaaa tctataccca
     3601 tattatacgg cagaaaagaa aaattgcaaa cgcttttttc attatttttg aattctttgc
     3661 tcgatttcgc tttttgtttg cttgagggca ctgattattt gttcttttgt ctcatctgtt
     3721 aaacgatatt tcggcatact tacactaaat gcaccaatga tttgttcgtt tttcacgatt
     3781 gaggctccta tacaaaaaat atcacgttcg atttcttcat cgtcaaatgc gatgtgggtt
     3841 tcttttaccg tttggatttc ctttttcaac cgtaatgcat tggtgatcgt atattctgtg
     3901 taagggatca acggatgctt ttcaagataa tcagtgtact gctcttcact gaattctgct
     3961 aatacggctt tgcccatcgc agaattgtat aatggtcggg tgattccgat ttttgaagac
     4021 atgcggatcg tttgatgttt tggatctagt ttgttcacgt acaagatctc attgttgtcc
     4081 aataccccta agtggatagt ctcatcgatc ttttgctgca gctgttctaa aaacggtaaa
     4141 gtcaactcta ccaaatcgat ttgttcaatg tttttattcg cgtagcgaat aagctttgca
     4201 cctaaacgat agtttttctc tgaattttta tttacgtagc cgatcatcaa caaagtatct
     4261 agtattttca agactgtaga agaggtcatt cccacaccct ttgcgatcgt ctgcaaagag
     4321 acatcttggt tttccgctaa atagtctagt attttagagg ctttgatcaa tacagttcca
     4381 taaggctttt tattttccat ataatattct cctatttctt tataagaaaa attatagcgg
     4441 aaataccatc aatttaaaag agatatccag atataaacta ccatttctct ttacaggcaa
     4501 tttaatgttg aagatccgct tcgtttttac atcaaacttt gatataaagc aatgatttct
     4561 tctactgtcg tttctcttgg atttcctggt gtgcatacat ctcgcaaagc atcttctgca
     4621 attgctggaa tatctgcttc ttttacacct aattcagaaa tggtttctgg gattccaacc
     4681 gctttgctca accgatcgat ctcaccacaa gccgcttctc ttacatcttc taaagacatt
     4741 tcagctgctc ctttaatccc tagaacaaga gcgatctcac ggtacttttc tccagtatat
     4801 tctttgttat atttcatgac agttggcaaa agtgctgcac acgctacgcc atgaggaatg
     4861 ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca gtcctaaacc gacatttgag
     4921 aagcccattc ccgcaatata ctgtcctaat gccatttttt ctcgcccatt ttgatcgcca
     4981 tctacagaaa cttccaaaga atggccgatg atttcgattg cttttaaatg cagcatatca
     5041 gtcatttccc aagcgccttt tgtgataaac ccttcaatag catgcgtcat agcatccata
     5101 cctgttgatg cagccaattt tttcggcatt cccatcatca tatcgctgtc gatgaacgcg
     5161 acgattggga tatcatgagg atcaacacaa acaaattttc ggtgatttac ttcatctgtg
     5221 atgacgtagt tgatcgttac ttctgctgct gttccagaag tcgttggtat agctaatata
     5281 ggtaaacatg gatttttcgt atcagcaaca ccttctaaac taatgacgtc tgaaaactct
     5341 ggattcgtca aaataatccc aatggctttt gccgtatcga ttggcgaacc tccaccgatt
     5401 gcgatcaaac agtctgcctc tgcctcttta acaaaagcca ctccatcttt tacattttgg
     5461 atcgttggat taggtacgat cccgtcgtat aaagcataag cgattgctgc tttatctaga
     5521 agttctgtta cttttgttgc tataccgtgc tcgatcaatc ctttgtcagt aatgacagct
     5581 gctttgccga atcctcgctg ctgaaattca gggacgatcg actcaatcgc tccttttcca
     5641 aaatacgatg tttcatttaa aatcattcga ttactcattt tctattcctc ctaatttgta
     5701 ttatttttca caataaaaac gaaagaaccc caagcaaccg gttgcccagt tgcttgttgc
     5761 tacagttcaa agactggcat caattcccaa ctgactggag aggcatctgg atttgtctcc
     5821 atgatatctt ccatgtactt ccaccatttt tgattgatag atgtatttgc cattttttcc
     5881 cataactttt catcttcgat cactaagtaa ccaaataaca aacttgtact cggatcaaga
     5941 tagatcgaat agtcgatgac cccgtgttca cgaagtgctg tacgcatttc tggccaaagt
     6001 tcatcatgtc tttttttgta ttcttcttgt ttttctgggt aaactttcat gcaaaaagct
     6061 tttttcacca tgtcagacac ctgctttcgt atgaacttcc tccaaataac ctgcttttgg
     6121 agtgacacca aacgcatctg ctaaatgcca caggttttcg tccgtgattg tttgcaagat
     6181 tggtccttgc gctttgacgt acgtatagac ttctgctgcc ttttctgctg tctcgatcaa
     6241 accaaagact tcatccatgt cttttcctga cccgtagatt ccatgttgtg gccataaaac
     6301 caatcgagtt tctttcattt tttcagctgt tgcttcccca atctcatttg ttcccggaac
     6361 catccacgga atgatggaaa ttccttctgg aaaaacaact aagcattccg tacacatttg
     6421 ccatagcgta cgactaaagg aacgttcgtc taattcatgc gtaaaactca tcgctaatag
     6481 atgactagca tggcaatgca taacgatccg attatctggg tctacttcta aacgggcgat
     6541 atgactcatg aaatgacttg gcaactcgct tgtaggaagg cattcgtttt ccagccccca
     6601 caaaagttcc agtgttttgc catcttcagc aactcgaacg attccaagat tctcggcagg
     6661 ttcgctcgct acatttttaa aatatcttcc tgaaccagtt acaataaaat agcatcctgc
     6721 taaacggctg gcatcaaaaa tcatcgagat gcggcggatc actttttcag gatccaagaa
     6781 cggtgtgatc tcttcttctt ttagtaaata agaaatattc ccgccatttc tttcatccca
     6841 tcccaagcga taaagattcg acgtcgtttc gatcatttct ttcacataag gcgcttgtaa
     6901 aatatctatt tttttcaata tagccacact cctattttct taattccaac acgtcttttt
     6961 gatattcctg gacttcttcc agccaatcca aaccaactgg aacttgattt ttgtaacaga
     7021 aataattcca tacatcggca tacgggaaat ctttcaattc ttccgtataa gccattcttg
     7081 aagtaaagtc aaaagccagc tccatttctt tcaatcgctc aactggttcc agcatagctt
     7141 tcagcagtgc tttttgtgta tttctcgtac cgatgaccca agcagcgata cggttgatcg
     7201 ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa caattcgtta cggactaatt
     7261 ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac atggtctgaa tcccaacgca
     7321 ctggacggga aacatgcaac atgattcctt tgctgaataa agctaatgaa gataatttat
     7381 tggaaatcac ttctgttgga tgaaaatgtc cagcatctag acaaacaagt ttgtcacgag
     7441 tcaacccgta acccatataa aattcatggg aaccgactgt ataagcttca gcgcctaaac
     7501 caaatagttt gctttctact gcatcttgta cataccgttc atctaactct tctgtaaaaa
     7561 tttcatcgag tgattccatt aaacgttccc gtggggtaaa acgatcaatc ggattatctt
     7621 tcatcccatc aggtacccaa aagttattga tacttgtttg cccagtttct ttcccaaagt
     7681 actccgcgat tttgcggctt cttttcccgt gttcgatcca gtattcacga acttctttat
     7741 ctggatgagc caatgtaaag ccgtctttca tcattggatg cgaaaagaaa gtcggattga
     7801 agtcaagacc gatttcttct ttttttgccc actctaccca tttttcaaaa tgtttcggct
     7861 caatttcatt tagatcgaca gattcttctg tatccaaata gatagcatga aggttcaatt
     7921 tgtgtttgcc cggtatcaac gaataagcct tctccagatc ttgtctaagc tcttcaggtg
     7981 ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc accggtcaat tcgccatccg
     8041 gtgtcaagaa acctttgaca tcatctcctt gccagcaatg catagaaatt ttgatgtcct
     8101 gtatcttttc tagtacagca tctgtatcta caccgatagt tgcatatttt tcttttgctt
     8161 gttcgtaatt tctttcaatc gtgttcatac gattactcct ttgattttat tcacgtacca
     8221 ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt tcacatggaa acgactgttt
     8281 gattacattt cttgcttgtt ctatattcga aaactgacct gtactgagca tttgcatggc
     8341 aacattcccg attgctgtgg cttcactagg tcctgcttct acaatgattc cggcataatc
     8401 tgctgtcaat tgattcagta gacgattatt cgatccgccc cctacgatgt gcagcttttc
     8461 caatttcgtt ccagttaagt gttctacttt gatcagctct tgtgcgtaac aaagagcaag
     8521 attatcatac acacaacgag cgatagctcc cgatgagaca gggactggct gctctgtttc
     8581 aaaacagtat tgctgaatct cttctatcat atttggggga ttcaaaaatc gagggtcatt
     8641 gatttccatc acttgctgca gcggtttttc ttttgcagca agctctgcca attccgcata
     8701 actgtactcg tactcttgca ttcgggcaac ttcctggatc aaccacatcc ccatgatgtt
     8761 tttcaaaaaa cgcaccgtat tatgggcacc ccattcattc gtataatttt cagaaaaagc
     8821 attgcgagaa acattggcga tctttgtttc aatccctaaa agcgaccatg tcccactgct
     8881 gatataaccc caacgttctc cttgacctgg cgttcctaat acagctgacg ccgtatcatg
     8941 acttgctact gtgataaagg tcacatctgg caaatcatat tttgaaaatt gttctttttg
     9001 cagatctcct agtatcgttc ccggctctgt caatggtgga aataattcct tcgagacacc
     9061 gattttttca agaagttcta tttcccattc ttttgtccct gcattcagca gctgcatagt
     9121 agatgcatta gttttctccg tcaccatgtt accagtaaag acatacccta aataatcagg
     9181 tatcagtaac aatttatgtg cttttgccaa attttctttt tcttcagcaa acaattggaa
     9241 caaggtattg aaaggctgga tctgaatccc tgtctgctca tacaacttat ctagagaata
     9301 gatttcagaa aactgttcta ctgctccatt ggtacgcttg tcccgataag caatcggatc
     9361 atttatccga tccccttgtt catcaatcag acagtaatcg actgcccatg tatcgatccc
     9421 cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca aatccaatca aaatctcttt
     9481 gataagataa tcgatcttcc agcgatcgtg accgtctttg ttccgaaagc cgtttttgaa
     9541 ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc ctcgtacttt tcattagtcg
     9601 tccgcttgat gcaccgatat cgatggcaac aaatgttgga ttcatttctc cacctctttt
     9661 actttattgt tgattagcac tttattaata aagcgttatc atttattaac aatgctatca
     9721 taatacagaa tcttatattt tgcttcacac tattttggta tataattgca ttatcttgac
     9781 aagttgggag tgtttacatg gattactggg aaaaagccaa ccactcatgg tcggaagact
     9841 caaaaagagt gatcttgacg cctacacaaa gaagcaggaa tttatttttt tatatccaag
     9901 aaatcggtta cttcaaagca ccaatccctt actatactga acgagcacat ttaccttctt
     9961 acttgataaa gtttactttg tctggcaaag gttcgcttac atataatgat aatcagcaac
    10021 agctaaaagc tggagactta ttttttattg actgccaaga atatcagcgt tatcaaacga
    10081 tcagcgaaga accatgggaa atggattgga tccatttgaa tggtcagaac gtccaatcat
    10141 tttatcagga gttcacaaaa gacggccata acaccttcca ttcaacaggg gatccatcag
    10201 aaaacaagat ccaccatttg atcacagaat tgatccacat gcaacaaggc acgaatgccc
    10261 gtaccgattt ccagtcctct gtagctatcc acgagttgct gaatgaactg attttgcaaa
    10321 agtatcagct agatttcgaa gaagaggata ttcccgccta cgtttacaaa ctgaagcaat
    10381 acttagacga acatttcaaa gaaacgatta cacttgatca attagaacaa caattcttat
    10441 tgaataaata tcagctgagc aaagattttt ccaaatttat cggttctcca ccaatcgatt
    10501 atttgatcag caaaaaactt tcttacgcta aagaccttct tcggtataca gaatttacgg
    10561 ttcgaatgat ttccgaagaa atagggattg agaattttgc ttactttagc cgcctgttca
    10621 aagcaaaaac aggactatcg ccgagagact ttcgtaaatt cagctgattc atataatatg
    10681 tacgtcattt tttcaaaata ttttgaaata tatagataaa aataaaataa ccgcagtttc
    10741 tgcttctatg taaacaacag cagctactgc ggttattttt tagtgatcat tctcattgtt
    10801 catcttcaaa atgttcataa tgaaccaact gtttctgtgt atcacgtttc aaaaattctt
    10861 taggcgttat gccgaactct ctcttgaatg ctttcagaaa atgcggataa gaagaaaaac
    10921 cacttttact atatacagca ctagagctgc ggtattctcg caacaagtgc ttggcataga
    10981 tcagcttttt cttcattaca tactgatgaa acgtaaatcc agtgtacttt ttaaattctc
    11041 gtgtgatatg gtatttgctg acaaaaaact gcttttccat ttcctctaaa gaaagcggat
    11101 gagataaatt ttttgagata tattgtaaca tttgttccac acgctcatta ggaactacag
    11161 cattctgttc ttgcgcactc tcttcttttt gtacaacttg attgagaaaa atcaagtatt
    11221 ctaccagagc ttgttcatat tttatgtctt caccgtaaat ttttttatca ggaggatggt
    11281 cgataaatga taatttttgc ttcaatactt cctgttctat ttgcaataca cgactcctct
    11341 tctctcccat tggttgaaaa caagccgata gattagacca tggcgtggaa cgactttcta
    11401 gaaaagaagg ggtgacaaag atatagacac gctcgaatac atctgaagat tggcggacga
    11461 tacgatgcaa atcattggaa tggatgagca gcacacttcc tgctttgatc gtaaattgtc
    11521 gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc atggatctca tagaaatcat
    11581 gatgatgata gatcgtttta ttttggctga cgacatcttt gacatgatag atttcatact
    11641 gatcagaaac catcgtattg acttgttcac cgctgatttt cttttggagc atgtgccgca
    11701 cctccttttg ttcacttatg cacaatttta ttataccacg ttttttgtaa tcaaaaagag
    11761 ctgcttttat atactcttat aaaaaagaag agacgtggca tcttctagcc ccgccccctc
    11821 cattctaaat attagttaac caacgtcttc tttacattat tcaagaagac ttcttccata
    11881 acgaatccca tcatcttttg tttcttcaag aaccacatac aggtacggac gattcttcat
    11941 gatgatttct ttgatttttg tgtactcgat cacaccgtct cccaaattta ccggcacgat
    12001 tttttcctgt tcgacacgaa aatctttcaa atgaaccgca catatctttt caccaaaacg
    12061 ttcaaaagct tcttctacca attggacttg ttcttgataa tcactacttg tgatcaaatt
    12121 ggtcggatct aaaatgattc ctagatagtc cgaatctact gcttggatca gctgctctac
    12181 ccttgctaaa gaatacaccg gatgattcaa acctggttct atccccacca tcatttggta
    12241 tttttctcca gcttttacca agcgacggat aacggacaca gcttcagcaa atgcttcatc
    12301 tgtgaaattt tcttccgtat attggatttc cggcaaaaca catccagttt ccgaagcaac
    12361 catcgacgca ccaaaggaag aagcatagcg aagatactgc tcgaactttg tcagcaactc
    12421 ttgtcgtatc gttaaatctg gatgaatcat attgatatag caacttaaaa ttcctacggt
    12481 cacttcttgc ttttctaaaa ctcgtttgac ataatttccc atcccagaat tgatctcatt
    12541 tgcgccagaa ggtgtttcag gaaacgaaag acctaaggcg agctgcagtg tatgaatccc
    12601 ttgttctttt gttttttctg caagatcttc tatcgtttgg acttcggaaa gatcatgtcc
    12661 tctaattgta atgttcaaca taactattct cctaactcag catattgggt agccacatga
    12721 cgatttgcgg gaagtaagta atcaaaaaca agacgactaa gataacccca taaaaaggaa
    12781 gaagcggttt cagcattgcc tctgctttta cgcctcctac acttgcaccg acatatagtc
    12841 ctgtacctac tggcggcgtg atggagccta cacacagatt catgattgaa aacaacccgt
    12901 aatgtacagg atcaacccct acacttgtga cgattggtaa aaatattggt gtaaaaatca
    12961 agatagcagg cgccacatcc ataaacatac ccaccagtaa taacacaaga ttgactaata
    13021 acagaataat aaatttatta tccgttacgc ccataatcaa cgaactaatt gcctgaggaa
    13081 taccggtaaa cgccatggcg aaagacatca tggaagaagt agccaataag aacatgatcg
    13141 tccctgacat tgctactgat tgaaacaaca tattaggtat atcttttagt tttaccgttt
    13201 tatagtaaaa catagcaatc aacagggagt aaatgacagc tatcgctgaa gcttctactg
    13261 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
    13321 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
    13381 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac aaagcgccta
    13441 cgagataacc tcccataaac aaagcagaga tcgaagcacc accagcaaca agtgaataca
    13501 tgataaaagc agtacttgga ggaatgacca tacctgtcgg agctgacgcg atattgaccg
    13561 ctgtcgcaaa cttccggtca tatccttctt ccacttgctg agggatcaac actccgccaa
    13621 tcgcggtaga agcggcaatg gccgagctag agatggaacc aaataaagca ttccctaaca
    13681 cattcgtatg agccaaagcc cccggcacgc gcccgacgaa caacatcgca aaatccacta
    13741 gtttcttagc tatccctcca ttattcatga taatgcccga gagaacgaaa aacggaatag
    13801 cgatcagcga aaaactattg atacttccca ccattttttg agctgaacta aaagcagaaa
    13861 catcaaacgg tataactaac aataaagtca tcatcgatga gaatgcaata cttatagcaa
    13921 tcggcatccc taatacaagc aacacaacaa atacaaaaaa taacactaat ccggcttcta
    13981 ttgccatttt attcctccat ttttatattt tttcttgaat tgctggcttg ttgcttaatg
    14041 tatcgattag attgatcagg ctatagataa tgatgagtac ccccgataca ggtactgaca
    14101 gataaacata tcccatagga atacttaaag aaggcgagat ctgccccatt gtcaatgtga
    14161 cagcattcca tccgccatac accatgatca tcgcagcaaa taccagaaat aaactttgaa
    14221 caacaacttc taaaatttta ctttttttgc cagttaattt ttcacttaaa accgttacgg
    14281 ctaaatgtgc tttttgtccc acaacatacg cggaagacaa catggcaaac cagacaagac
    14341 cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc taaaaccgtt ctggaaaata
    14401 cttgccataa aacaacagcg accatcccaa caagcaaaac agctcccagg atctctaata
    14461 ccttgtttaa aatcattttc atcacagcta cgctccttgc tcttggatct tttgataaag
    14521 gtctttgtag gtaggatcgt ttttgaatat atcatgaagt ggggcaactg cctctcgaaa
    14581 cggtgtatta tctacatcat tgaattggac tccatgttct tcttgtgcta aagctttttc
    14641 ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc gttgattctt tcgctgcttc
    14701 ttcaatggct ttttcctgct catcagttag ctgttctttg atggcatcat tcatgatgat
    14761 gatatctggc acacgtgtat gctcatcata ggaatagtat ttagccacac caccatgtcc
    14821 agctgtatac aagacaaatt cattgttttc tgatccgttg atcaaattgg attgaagaga
    14881 cgtgtacact tcatcacttc ccataggtac gggcgaaccg cctaaaagct ctaccatttt
    14941 gatagcggtt tcgctttgca tcacacggat cttctttccc tccaaatctt ccggcgtatg
    15001 gatcggaccg tctaccatat aaaagctgcg ctgaccagaa tcataatagg tcaatccggt
    15061 aaatcctaaa tttttggtcg attggtagat tggctccatt atttcttcat tatccatcac
    15121 cttaaagaaa tgttcttcat cattgaatag ataaggaact gaaaaaatcg aataatcttt
    15181 agagaaactt tccaacgctg aggcacttac ttttgtgaaa tctactgctc ctgactgtgt
    15241 caattcgatc agttcacgtt ctccacccag ttgtccatct gggaaatact ggatctgtac
    15301 ggaaccatct gttttttctt ttactaaacg cccaaattca ttcattgcat caattactgg
    15361 ctggctgttg cttgcataag caaagcgcaa ggtaactatc tcatttacat ctgcatctgc
    15421 gacagttgcc acactagaat tatttccgca gccgcttaga cctagcagac ctaggctaag
    15481 cgaacttgct gcaaacagca gtgatgtttt tttcatatgg gtttcctttc ttttaacaat
    15541 aagagacgag caattctgtc aaacaaagca ccgttaacga ctgtccataa ggcattgctg
    15601 tgattccgat attccgataa aaatctaaac tgtcccccat tcctgtcccg attgaaacgt
    15661 tttgaacttc tcctttttca tcgatttgtt caagcaatcc ttggatagca cgataagcga
    15721 cttctttata ttcttgtggt aaataacgtt tatggaccgc tttcaaaata ccgtaagcaa
    15781 atcctgcagt tgcagaagat tctacgtaag aagaaggatc atccaacaac gtatgccata
    15841 aaccgctttc agactgataa gtttttaatg cacgcacttg tgcttcaagt gtttcgatca
    15901 ataatttgcg caatgcatct tctttcgata attctaagat ttcaatgatc tctggaatag
    15961 caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc ggcatagtta tgatttcctt
    16021 caaacgtcca gccgtgatac cacaatcctg ttcgtttatc catcaagtat ttgatatgga
    16081 tcataaactg gtaacgagct tcttctaaat attctgggcg attcaacagt ttcccgattt
    16141 ttgccaaagg aagcactgtc atcatcaatg tatcgtccca caactgattc ttattttccg
    16201 gtccatatgt ggcatgctgt aacccgtctt catttgtccg aggcatgtca ttcatgaccc
    16261 attctgccca ttgttctaaa taaggcaaat agcgtgaatc tttcgtgtct tcatacaagt
    16321 aagccatagt caatagcggt gccattgtat tgacgttttt gggcggagcg ccttcttcca
    16381 tacgatcctc aaaccattca ttgaccactt gataagcgcg aggatttttt gtcatctggt
    16441 aatttttata aataccatat agaccgacac cttgtggcca attccacact gtccagcttt
    16501 tgtcatctac tttcagtcca tcaaagtcca acaaaaattc accattatcg tctttgatcg
    16561 ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac taattcttta ggtacaaaaa
    16621 tctcttcttt aataatcatc tttacgccac ctttttaaac gctttcattt tatatcctta
    16681 atatagcagt aataccgtcc actataaata tcgctttttg aacaaaaact gtctaaaatt
    16741 gacctaaatg ttaaataaca aaagtaactt tcagttactt ggaaattaaa aaaagaactt
    16801 ggagaaacaa cgttttccaa gttctttttc atctattttc ttatcttttt tctacgcttt
    16861 cttttgctta tccgttgtaa gcatcaatga tgatctgttt caattcagag atcaatggtt
    16921 ctttcggatt agctgtcgta cattggtctt cgtaagctaa ttcagccata cggtctacgg
    16981 ttgtatctag tgtttcttgt gtgactcctt gagcttttag actcatgttg atgccgacag
    17041 attttcctaa ttcataaaca gccgttgcta aagcttcgac taattcctct gttgtttcgc
    17101 ctttcagacc taagaatttt gcgatatctg cgtaatctgt atctgcacgg aaatagtcat
    17161 atttagggaa catcgcatgt ttttgtggat cttttgcatt atagcggata atatgcggta
    17221 aaagaatcgc atttgtccgt ccatgaggga tcccgtattc cccgccgatt ttatgtgcga
    17281 ctgagtgaca aatacctagg aatgcgttag caaacgccat accagccatt gtagaagcgt
    17341 tgtgcatttt ttcacgtgat tccatatcag gtgtttttac tgatttttct aaatagtcaa
    17401 agactaactt gattgcttgt aggctcaatc cacgtgtata atctgaagcc atgactgaaa
    17461 cataggattc gattgcatgt gttaacacat ccatcccggt atctgctgtg acagatgctg
    17521 gaactgacat gacgaattga ggatcgacga ttgcgacatc tggcgtcaat gcataatctg
    17581 ctaacggata tttcacatgt gtttcactgt cagtgatgac cgcaaatggc gttacttcag
    17641 aaccagtccc tgatgttgtc gggatacata cgaattgtgt tttttctggt ttgtcgatct
    17701 tatatgtccg tttacggata tccaagaatt tttgttttgc accaaagaaa gatgtgtctg
    17761 ggtgttcata gaacatccac attcccttag ctgcgtccat tgcagaaccg cctccaagag
    17821 cgattactgt atctggcttg aagtctgcca tcagtttcgt tcctgcgtat acggtatttg
    17881 tcgatgggtt cggttcaaca tctgagaaga cttcgatctt cacatcattt ttacgtcgtg
    17941 ccaatacttc acgaactgta tcgcagtaac cgaattggac cataccaggg tcacaaacga
    18001 tcatgacacg ttcaacgttt tccattttct ctaagtacaa tagagagttc ttttcaaaga
    18061 agatttttgg cggtaatttg aaccattgca tgttatttct ccgtttcgct acagttttga
    18121 cattgattaa gtttacagca gagacgttac gagaaacaga gttcttacca taagaaccgc
    18181 atcccaaagt caatgacgga atcatttcgt tatagatatc accgatgccg ccttctgcag
    18241 atggtgtatt cactaaaata cgacaagctt tcattcgaag accgaatttg acatgcaact
    18301 cttcgttttc tgaatggata actgctgtat gtcctaatcc tccaagattc aacatacctt
    18361 cacacaaatc aaacgcatgg tccgtgttgt tagctttgat catggcaaga actggagaaa
    18421 gtttttctct tgatagcgga taatctgggc ctacgccttc caattcagca atcagcattt
    18481 tcgttccttt tggtacactg attcctgcca attttgcgat ttccatcgca gaatgtccaa
    18541 cgattgatgg attgaccgca tattttcctt cgttcatcac tgcatcttct aatttttgca
    18601 attcatctgg ttttacgata taaacttggt gtgcttggaa ttctgctttt actgctgcat
    18661 agatttcttt atccacgatc acagcttgtt cagaagcaca aatcattcca ttatcaaatg
    18721 ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt tgctgttttt tcaatataag
    18781 ctggtacgtt acctggacct acacctaaag ctggttttcc tgtagagtaa gcagacttga
    18841 ccattccagc tccacctgtc gctaagacga tcgcgatacc tggatggttc attagcatag
    18901 aagtcgcttc gatcgaagga tgttcgatcc attggataca attttcagga gctccggcag
    18961 cgatagcagc atcacgtacc actcgtgctg cttctgcaga agatttttgg gcacttggat
    19021 ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat cattgatttg aaaatcgttg
    19081 tggaagtcgg attcgttgtt ggagttactc cgcaaaccac accgactggt tcagcaattt
    19141 caatcaaccc tttttggaca tcttcattga tcacaccaac tgttttatca tgtttgatat
    19201 tatgccaaat atattcggac gcatacatat ttttgattgc tttatcttcg taaacaccgc
    19261 ggcctgtttc ctctacagcc atcttcgcta atggcatatg ttgatccaaa gctgccattg
    19321 ccatttcatg cacgatatgg tctacttttt cttgatcaaa gctttccatt tctttcaatg
    19381 ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg tacgtcaatt gtcttcgttt
    19441 tttctttttc tattacttta gccatcatta tcctcctaat gggtcatggt tatttgttaa
    19501 ataattcaca tgtatatcat acaatattta tttttctatg taaacacttt tttgtgattt
    19561 ttttcacagt tatttttaaa tataaaattg ctttatttac aggtgtttgt atagaaaaat
    19621 atataaagtt atttattatt atttatatga cacacccgat tattcaatat tgagcaatat
    19681 atcccatgct cttatctctt tcttttttga ataaaaaatg aggtaggaca tctttctgcc
    19741 ctacctcgtc tttaaataag ctttgatact tatttttctt ccttctcttc tgtctcttcc
    19801 agcgtttctg ttgtttcagt ttttacttca actgtctcag ctggttcttc tgttgtttca
    19861 acagtagtcg ttgcatcatt tgttacgaca gtccctggtt tgaccgtttt gattgctgca
    19921 cgatcgtact caaggaagat cccttcgcag tctaaaacaa ctgtccgttt gtcattgtcg
    19981 atttctgaaa caactccatg taatccgccg atcgttacga ctgaatcacc tggtttcatg
    20041 ctatctaaca agttttgacg ttcttgctgt tgtttctttt gcgaacgtga cataaaaaac
    20101 cacattgcta atagcgcgat aaacatcaca agcattggta atgttcccat ttttttcacc
    20161 tcactaattc tgtatctaca aaaaacactt tagcagaaaa atgctgataa cactagtttt
    20221 ttacttaatt tttaaaagat tttcctttag aagctttttg cgttttcttt attgaacccg
    20281 tattcttcaa aaaaggcttc tctaaattca agcaaattat catccatgat tgcttgacgt
    20341 acttgtttca ttagattcaa caagaagtat aaattgtggt aagaagtcaa acgaatccca
    20401 aatgtttcat cacatttgat caaatggcgg atataggcac gtgtatagtt tttacatgta
    20461 taacaatcac atttctcatc aattggacgg aaatcatggg catatttcgc atttttcact
    20521 actagacggc cttgagaagt catacatgtg ccatttcgag cgattcgagt aggcaagaca
    20581 cagtcaaaca tgtctactcc acggataaca ccatcgatca atgaatcagc agcaccgaca
    20641 cccatcaagt aacgtggttt gttttcgggg atcaacggtg tagtgaattc taatacacga
    20701 ttcatttctg atttaggttc cccgacagat aacccgccga ttgagtaacc agggaaatcc
    20761 atcccgacta gatctttcgc gctttgacga cgaagatctt caaaccctgc tccttggatg
    20821 attccaaata atccttgacg gtctggatta gcatgagctt tcaatcctcg ttctgcccat
    20881 cgagacgtac gttctactga ttttttcacg tagtcgtaac tttcatcgaa tggtggacat
    20941 tcgtcaaaac tcatcatgat atcagagccc aatttatttt ggatgttgat tgctttttct
    21001 ggtgataaaa acatacgcga accgttcaag tgatttttga aatgcacgcc ttcttcttcg
    21061 atattccgca tatcacttaa tgaaaaaact tgaaagccgc ctgaatctgt taaaatcggt
    21121 tgatcccagt tcataaattt atgcagtccg ccagcttctt ctactaagtc ttcaccaggg
    21181 cgtagccata aatgatatgt attacttaaa ataacgccag cacccatttc tttcaactct
    21241 tctggggaca ttgtttttac tgtagctaat gttcctactg gcatgaacat tggtgtgggg
    21301 aaagttccgt gtggcgtgat caattcccct aaacgcgcgc cagtatgttt ttctttcttg
    21361 attaaacgat agcgaatggc aggttgcgtc attcatttcc ctactttcta tataaaatat
    21421 cttgacaact cccaccatca taatcgtttc aagtgctttt tgcaagttcc ctcggcttcc
    21481 ttctacttta tttaatattc ttttttttat ccttttttta tgaataacaa aaaaaagaaa
    21541 aacattatga cagaaatatt ttttgataca tggtatattg gtgttattaa agaaaaggag
    21601 ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag tctaaaagga
    21661 cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat tgctactatt
    21721 cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg atcagcgagc
    21781 gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg aattatttcc
    21841 agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga tattttacgt
    21901 ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa aggtttgttt
    21961 atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt atggggtttg
    22021 ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta ttttatttac
    22081 tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat cactgcaagt
    22141 cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc atttatcggc
    22201 tggcatatct tagcattagc aactgctggt attggttatc tatggctcag cccatacatt
    22261 tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa gagagtgtga
    22321 cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa aagaaacatt
    22381 cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt tttgtatagt
    22441 tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc tgcttccggt
    22501 aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa aatccctacc
    22561 aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt aaatggcagc
    22621 aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa agctttattg
    22681 gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac atgcagaatc
    22741 gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact tgcgtctacc
    22801 catgggctga tagtatcgac ataactacct aaataataac caaacaaggt tacgatggtg
    22861 aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat cccttcattt
    22921 ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc taaagcaaat
    22981 cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt aaatggtgct
    23041 ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt acagcttagc
    23101 aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact gacagcgtct
    23161 acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg cgccacatct
    23221 gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg tgcatcattc
    23281 acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt gacgatccgg
    23341 atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg cgcatctaat
    23401 tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg atcattcaag
    23461 atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt gatcatgact
    23521 gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg acgtggtgga
    23581 tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc agaagaaata
    23641 tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag tgcccgttta
    23701 ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa gcgcggagca
    23761 agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg gtgaactgtt
    23821 gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata tttttcttct
    23881 aattctgcct ttgtatgata attctcttca acagccctta cgatggctac ttctgttgga
    23941 ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt acatagtgca
    24001 gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt cgttacttca
    24061 tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt tcctgttttg
    24121 tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg tcggatgatc
    24181 gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac aataacagtc
    24241 aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaacat ctcaaggagt
    24301 ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa agcgatcata
    24361 ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg catagtattt
    24421 tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc agtgacgatc
    24481 gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag ttggtcacct
    24541 aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc actttctcca
    24601 gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc atcggcagaa
    24661 atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc tgactcaaca
    24721 gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc ttgcaaggat
    24781 tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt gatcacaacg
    24841 atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa tgcggtataa
    24901 aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa gcttcgaaag
    24961 aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc ctgctttttt
    25021 cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc tgttaccttc
    25081 tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta aaatcttcgt
    25141 acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca aataaagggc
    25201 ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc aaacgctacc
    25261 aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac agaagaattt
    25321 ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac ccagacatgc
    25381 gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca cgaagtatcc
    25441 gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga aaattgaata
    25501 ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt gtttaacatg
    25561 aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt tctgtttatt
    25621 tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt ttctttgtca
    25681 taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata accttatttc
    25741 acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc atgctggtaa
    25801 gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa agtcgatttt
    25861 ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc aggcgtaatg
    25921 aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt tccaatcgtt
    25981 tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt tttacgtact
    26041 tggttcaatc gctcagccgc ttcttccgtc aaacggtaga attcactatg catatggtgt
    26101 tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg tagtgtcaga
    26161 tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt aaaatgcaat
    26221 ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg atagcgatct
    26281 gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa tgattcaaga
    26341 atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg ttccaattct
    26401 tctttcacta ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc aactttggca
    26461 cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt tagaagaagg
    26521 acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg cagaactcgt
    26581 gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc atggaaatgt
    26641 ttatcttcca tctctccagt tttccgatcc aaaacaagta aacgagaatg atcgcggtct
    26701 tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc ttctgttgtt
    26761 aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt tttcgttctg
    26821 ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa aaaactaccc
    26881 tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc attgaaaata
    26941 gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat aaaggtggaa
    27001 aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt tgttttatcg
    27061 gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg actgatccaa
    27121 cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca gtggaattgc
    27181 gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg acgatccgtg
    27241 aaaatatcac tcttgtccaa aaattgttga aagtcagtca agaagaacgg aataaaacag
    27301 ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc tacccacatg
    27361 aattatctgg tggtcagcag cagcgtattg gagtcgttcg tgcccttgca gcgaatcaag
    27421 atatcatctt gatggacgag ccattcggtg cgcttgatcc aatcactcgt gattccttgc
    27481 aagaccttgt caaagattta caagagcgtt tgggaaaaac gatcgtattt gtaacccatg
    27541 acatggatga agc
//
LOCUS       DADXTH010000033        27296 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.033, whole genome shotgun sequence.
ACCESSION   DADXTH010000033 DADXTH010000000
VERSION     DADXTH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.033"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(643..1623)
                     /locus_tag="IWI97_001378"
     CDS             complement(643..1623)
                     /locus_tag="IWI97_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBC2462888.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1636..2514)
                     /locus_tag="IWI97_001379"
     CDS             complement(1636..2514)
                     /locus_tag="IWI97_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBC2462889.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2530..3330)
                     /locus_tag="IWI97_001380"
     CDS             complement(2530..3330)
                     /locus_tag="IWI97_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBC2462890.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(3605..3754)
                     /locus_tag="IWI97_001381"
     CDS             complement(3605..3754)
                     /locus_tag="IWI97_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462891.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            4722..5048
                     /locus_tag="IWI97_001382"
     CDS             4722..5048
                     /locus_tag="IWI97_001382"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2462892.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            5029..5964
                     /locus_tag="IWI97_001383"
     CDS             5029..5964
                     /locus_tag="IWI97_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBC2462893.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6593..6844
                     /locus_tag="IWI97_001384"
     CDS             6593..6844
                     /locus_tag="IWI97_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBC2462894.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            7386..7544
                     /locus_tag="IWI97_001385"
     CDS             7386..7544
                     /locus_tag="IWI97_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2462895.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            7616..8842
                     /locus_tag="IWI97_001386"
     CDS             7616..8842
                     /locus_tag="IWI97_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2462896.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(8931..9323)
                     /gene="rpsI"
                     /locus_tag="IWI97_001387"
     CDS             complement(8931..9323)
                     /gene="rpsI"
                     /locus_tag="IWI97_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBC2462897.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(9339..9782)
                     /gene="rplM"
                     /locus_tag="IWI97_001388"
     CDS             complement(9339..9782)
                     /gene="rplM"
                     /locus_tag="IWI97_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBC2462898.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(10301..10768)
                     /locus_tag="IWI97_001389"
     CDS             complement(10301..10768)
                     /locus_tag="IWI97_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBC2462899.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10888..12426)
                     /locus_tag="IWI97_001390"
     CDS             complement(10888..12426)
                     /locus_tag="IWI97_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBC2462900.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLSGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            12506..13192
                     /locus_tag="IWI97_001391"
     CDS             12506..13192
                     /locus_tag="IWI97_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBC2462901.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(13393..13920)
                     /gene="msrA"
                     /locus_tag="IWI97_001392"
     CDS             complement(13393..13920)
                     /gene="msrA"
                     /locus_tag="IWI97_001392"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBC2462902.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14004..14681)
                     /gene="pgmB"
                     /locus_tag="IWI97_001393"
     CDS             complement(14004..14681)
                     /gene="pgmB"
                     /locus_tag="IWI97_001393"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBC2462903.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(14678..15808)
                     /locus_tag="IWI97_001394"
     CDS             complement(14678..15808)
                     /locus_tag="IWI97_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBC2462904.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(15808..17991)
                     /locus_tag="IWI97_001395"
     CDS             complement(15808..17991)
                     /locus_tag="IWI97_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBC2462905.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17988..19013)
                     /locus_tag="IWI97_001396"
     CDS             complement(17988..19013)
                     /locus_tag="IWI97_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2462906.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19058..19885)
                     /locus_tag="IWI97_001397"
     CDS             complement(19058..19885)
                     /locus_tag="IWI97_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBC2462907.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19882..20949)
                     /locus_tag="IWI97_001398"
     CDS             complement(19882..20949)
                     /locus_tag="IWI97_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBC2462908.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20975..21811)
                     /locus_tag="IWI97_001399"
     CDS             complement(20975..21811)
                     /locus_tag="IWI97_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2462909.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21808..22677)
                     /locus_tag="IWI97_001400"
     CDS             complement(21808..22677)
                     /locus_tag="IWI97_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2462910.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(22747..24030)
                     /locus_tag="IWI97_001401"
     CDS             complement(22747..24030)
                     /locus_tag="IWI97_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBC2462911.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24044..25729)
                     /locus_tag="IWI97_001402"
     CDS             complement(24044..25729)
                     /locus_tag="IWI97_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBC2462912.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26004..27029
                     /locus_tag="IWI97_001403"
     CDS             26004..27029
                     /locus_tag="IWI97_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2462913.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7925 a         5678 c         4843 g         8850 t
ORIGIN      
        1 aaaaaatatg cggcgcacag aacgcgccgc atatcggact aagttttatt caaatttgtc
       61 ttcgccaaca caatttgacg aagagccttt tctttcagga agacttcggt aatttttagg
      121 agttctacct attcttttaa ccgtttattc tctttttcta agcgtgtaat ttcatttctg
      181 cgaatggatt tcatgacttc ctctaccgag aaaagtacgt tatccatatt tttcaacttc
      241 ttgaactcat caatacacgc tattagagcg agcttctagc tatagactaa cttctttaac
      301 agaaaaccct tcttccgtta ccagttttac agcggtaatt ttaaatattt catcaaatga
      361 acgtcttggc ataatccaga tcttcgcttt ctcttaattt ggtgtcaatt ttagtataac
      421 ccgtccaact tattttttaa ctaagtgccc ggtttttctt gacaactcca cccaaaagtt
      481 agattttttg gtctaagttt tagggtcgct tcattactag aattcaacaa gagatgtttt
      541 ttattttatt taaattatgg ggtcagtttc catttgatta caaacttctt tgttttcttt
      601 ttttaaacgc aataataaag gttttaattt cagccttttt aattagaaaa tcaattttcc
      661 caaattattt tctttagctt tcttaacgat ttctgtcgca actaccacat ctaatacagc
      721 cgaaccaact gttttgaaga tcgtgatttg ttctccactt gtccgaccga tcaattcttc
      781 attgatcagt tggcctagtt ccccatgata gctgtctctt tgaatatacc catcttgcag
      841 cggtgaaatg aagtccccag cttcttttag aacaccgtcc atggtatcaa aaataacaac
      901 atctgcggct ttgattattt ctcgtggaat ttcacacatt tccggtgtgt aagcaccgac
      961 tccattgatg tgcgcacctt ttttgaccca ctcagcagag aatgtcgcac gttttgaagt
     1021 ggtcacactt gtaatgatgt ctgctccttc aacgcattct tgactggttt ttgttgggcg
     1081 catcgttaca gaaaattgtt gcatcatttt ttcagcaaat tggctggcac gctcaaaatc
     1141 aatatcaaaa atccgaactt cttctaattt acgaacggtc aacattgctt ctaattgtga
     1201 ctgtgcttgt ccgccggttc caatcagcgc gccaatttta gcatcttctc ttgcaagaag
     1261 ttctgttgct gctccttgaa cagcgcctgt tcttaattgt gtcaaataag taccatccaa
     1321 acaagcggat accatacccg tttctggatc caatacgatc atcgttgctg gtacacttgg
     1381 taagtttttc ttgatgtttt caggatacac ggaaacgatt ttgacaccta gcgcgccttc
     1441 gccaccttct acataagcag gcatatataa gctttgtccg ttgctttttg gcacatcgat
     1501 attggtgcga agaggcacac ttgcctttcc tgctgaatac aatgacagtg cttttttgtc
     1561 cgcatcgatg gcttctctca ttgaaaaact tttgataata tcttcttttt taagaaataa
     1621 catttgaatc gctccttaat tagctgctgt aacttttttt aattgactgc ccaacatcgt
     1681 atatgcactg tatagtaaga ccaaaatcac gatccatttc aggatatagg tatctaaaga
     1741 tttgactagg aatacagcga ccaatacccc caatacaccg aaggttgaag taaaaagtgt
     1801 gatttttctg ctgtattcat taaatttaat gaattgaaca ctgccgatcg gtactgagaa
     1861 ggtacaagcc cccatcataa taggaaaggc tgcaactgga ttcaagccta gcaagtagac
     1921 agttaccatc gttaacgcat aagaaccaat gccgatattg ttcaatgcac catagacaaa
     1981 gagtaaaaat cctgccaaga ttaattttcc gccatacaat tcactggctg ttccgtttga
     2041 tggaatccag ttcatttgtc ctgcaaaaat caacaatgca gcaatgacca atcctatgcc
     2101 gacaaacagt ttgatcgttt tctctggtaa cttcacgaca aatcttggtc ctaaatatgc
     2161 accgataatt tggcagataa tacaaacagc tagtgtcttg atccctacgt tgatggcagt
     2221 gatgtaagac aatgccatga cggctaccgg tactacacat tgggtattca acgttccggg
     2281 tagttttttc atgctgaccc atttcaactt tggataaagg acggttccga tagcaaagtc
     2341 ggaaacgcca aatgttgaaa gaaagaagat gataaatgac gtgaagggca acagacgcat
     2401 atcccctggt tcttctttga aattttgttt gtttttgaaa aggtctctta gaaagtaaat
     2461 aataaaatat agattgattg cgacaattag tgtcagcaaa atattccgaa tcataaaaaa
     2521 ttcctcctac tagtaaatct ttctgattcc ctcaaagccc tcaccatatt ttgctttaag
     2581 ttcttcagga gattctgcaa tcacttttag tgtatatgca tcatgtttga catattcttt
     2641 tgctagattg accatggcgt ggttttttgt ccaagtgaca tcggttgatt gcagttcttc
     2701 gttaatggta ccaaacacga cttcttgctc atcggcgacg atgttgatcc atcttgctcc
     2761 aaagtcagca agtttgtctg tttcaaaacc atgaatataa tacttttcta agggaagatc
     2821 atattcatgg gtagaactga ataaaatcag tacaaactta tcaactcttt tttcagcttg
     2881 ctgcaatgcc tttagtagtg aatccgtgaa attctcatgc caaacttgaa tgagcaaact
     2941 ttcttttgcg tttttcacca aatgctctac tttatccaga acatactcga taccatcaac
     3001 tttccacagc atttcttctt catctttttc tttgatcatc cctagattct tggtaagaaa
     3061 agaaagatca tttttaacac ttttttctaa tttctctaaa aactcatttg ccgagatggc
     3121 atgataaagt ttaggttctg atttattaac gacaatcaaa ttctttttta atagtttttc
     3181 caaaatatta tatactttcg agcgtggcac acctgagatt ttacttactt catatcctgt
     3241 aagattacct ttttctaata gtgtggtata cactcttgtc tccatctctg aaaaatcata
     3301 ttttttcatg atttgaataa tcgtttccat tgattaatcc tcactttatt tttagtagct
     3361 actacggtaa caactataaa ttctttttga ttatctgtca agagttagtt caataacaca
     3421 catataataa ttagcttaaa aacaatcttt ttattaaaaa gaaacattat aatatgaatg
     3481 tttgtgaaaa aataatacaa cttagtcatt ctttacaacg atttatagaa aaacaacctc
     3541 tttaaatata aaaggtacgg gttactgatt acttgtaaat ctttacgttc cagttttatt
     3601 ctttctaaaa aatatactca cttcttttct tagacttctt tttttgattc tcctctttat
     3661 ggtcttgtcc ttttttgcct tcaaaatatt taaataaacc cctttcaaaa gcatttctag
     3721 gttttggaca ataaggaatg acttttttct tcatataaat acctcattta actatttgat
     3781 atcatcacat tatcaaaaaa aataggtttt ttattaaaac tatcttgacc tttcgggaga
     3841 tttcactgtg atttattttt attcccatac ttttttcgtt ggtcactttc gtatacctaa
     3901 tctaagcgat catcggcgac ggtgctttta caacgaatgg caggacaaaa caaggggcgg
     3961 aaaaactttt ttgcctattt cataaaaaac tttttcctac agcaatccct tgttttgtcc
     4021 taccacacgt tgtgaaccca aagcacgccg acgaacgctt agctatacga aaatgacctc
     4081 caaaaacaaa aaagtgaggt gaatagaaac aaaaagaaat tgaaatctcc caaaatgtcg
     4141 tccgcctcgg agacaacttg ctgctgaaaa tggttggtta attcatttaa gtattaaata
     4201 agataaatca tcaaattaat cggaaaaata gcatgattgg ccttatcaaa aataaactgt
     4261 aaaaacttat atttctatga gtggtggaac aatgctaata ccaaattaat ttatcgatta
     4321 cccacctgca caaaccaaag atagcattat taggaaacga atcaatcagc gaatccagca
     4381 ttataatgag gatactaaaa ataaagtttt cataattata aatccgcctt cctacgaaag
     4441 gcaccaatga ggtgatgaaa accttagagc gctttctttc tttttttatt ccgtgttacc
     4501 ctttccttgc aggtccctgg tacactacag aacccgagga ggtgagtggc ggggctgtat
     4561 agcggcaacc ccgcataatt atatggtgtc ggtcatccgt taaggcatca atgaatggcg
     4621 caaacccgta gcccgtaaac ttatctcttc cgaagttgac tcgatttcgc cggatgacca
     4681 gtccctacca aacctgtact atttttttac aggaggttgc tatgttcaaa atctttcgta
     4741 aaaattgttg tggcttggat gtccacaaaa cgtggatcta tgcgtgtatc ggaatcacag
     4801 atgcgaatgg tcgtacagtg tataagcaag ctcgcttctc ttcctttaca aaaggtctca
     4861 atgaattagc tgaatggctt gctaaataca actgtaagga tgtgtgtttg gaatcttccg
     4921 gtaaattttg gattcctgtt tttaatattc ttgaaaaaac ttgttttgtc acactggctc
     4981 atccgaagta caccaaaccc cagaaaggaa ataaaacaga tcgcaaagat gcaaaaatgg
     5041 atttgtgatc tctttatgtg cgacatgatc aaacccagtt ttattcctgg tcttgaaatc
     5101 cgccagcttc gtgatctaat gcgttatcgg acgaaactga ccaacatgaa aacaagtgaa
     5161 aagaatcgtg ctttaaactg cttaaccgtt tctaatctga aactagatga tgtttttagt
     5221 gatgtgttcg gaaaatcatc cacttctatc atccaatact tgttagaaca tcccggtgaa
     5281 tcatttgatg ttcgaccatt tatccatgcc cgttgtaaaa ctcccgttga agaaatccaa
     5341 gcggcagttg agggggcgat ttctgtacct caagcgatta agcttcgcca atgcctcaac
     5401 catattaacg agattgagaa gcatcgagaa gagatcaatc tagagattca agctcttgca
     5461 gaacccttct cccctgtttt ggaactactt cgtaccatac cgggcttaga cactcagcca
     5521 ataacagcga ttgcgattct ttcagaaata ggaccggata tgtccgtgtt tccaacttcg
     5581 aaacacttta tttcttgggc tggatgctgt ccaagaaacg atcgcagtgc ccgacgaaca
     5641 aaatcaacac gtatctctcg tgcagggcag tcgttgaaac cgctacttgt acagattgcc
     5701 aatgctcttc tcagatccaa agaacatcca gaatttaaaa atcgctatcg tagaatcaaa
     5761 gcgaatcgtg gacacaagaa agctatcatt gctatttgta agatgctcct gaccgctatc
     5821 tggaatgtct tatcaaaggt ggaaccctat tctgctaaag gatatctgga gccaaaacct
     5881 gcgaaagaag agaaagttct gacgcaatcc caagctctgg accttttaag aaaacgcgga
     5941 tataccatta tagatagtgc ttaaaccaaa gttacgttga ttcttcctaa atagactcgt
     6001 tgtcttccat ttcaaaaatt ttgaaaacgg taggcttatt tagtgtaccg ttcttcaggt
     6061 atcttcaaat agatttattt tcaaacttat ttcctttctt cttttactct cctatagctt
     6121 agcatttaag tgggcactcg tgtcaccttg ctagatagct atgaatgccg taataagcct
     6181 cctagtatat aggcagactg ccaattataa ctgattcaat ccacattatg ataaaatcca
     6241 ccttttcgtt tcatatgtgt tgaaatatca catatgtcaa aagtgcacga gtcttttcat
     6301 tcagtgcttt gtaaatactc aaaattattc cttttcactt taaaaaaaag ataattagta
     6361 caaaatcaga aatgaggaaa gtattaggat ggactagaaa ggagttgttg aatttgttgc
     6421 aacagattgg tcatttatca attaaaaatt aggtgaagtt ttattcatca ctgcagctgc
     6481 tgtttcccag agaataaaac gtctagaaga aactggtcag ttactgtatt atcaagcagc
     6541 aattgactac gaacaagctt gattactaac caaagcgttt atccacctgt tgattaaatc
     6601 aaatcaaaaa tcaggttttt atacgttcat aaaagcaatc tccagtgtac tcgaatgtga
     6661 ctgtatcata ggtccttatt cgatgttaat caaaatgggg tttccttcta ccgaatcatt
     6721 ggacagtttt atcaatgatt tatcaaagtt tggacgaacc aacacccaaa tcgttttctc
     6781 gactcttgtt tccttaatgg tttatcagtt ctggaatgtt tatcatgcgc ttttattaaa
     6841 atagaaaaga gcttttttca tgcgacaaag cagtgatcat ttattggcac atgcaatctt
     6901 tgtggatcca ttttttgaaa atacgatatt tagttttgac ccaaaaatct ttaaatacgt
     6961 atttgataat ttatcttaac aaccagataa ttttattcag caccgtatta tctatattga
     7021 attctcggaa tcaagataaa tttttttccg tttttgtttc ttttttaatc ttcttcttgt
     7081 tattaatagt gattaggatg atattcctat attgatcttt gataattaga tataattttt
     7141 gaataacgtt ccctgctatt cgagctagaa atacaagtac gccatgacct agttagcgac
     7201 aactacttag agatttcttt tgttctggga caacaaaatg ccatgacaat agcagtaaat
     7261 aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct atgtgcttac
     7321 ccaaagcggt tcaaaaattt taaactatta ttttaggaga tgattttata aaatcgaaag
     7381 cctacttgaa taaaaaacaa gcctgtgaat ttctaggaat tgataataat actttagata
     7441 agtggattcg tgtatataat tttccagtaa taaaaattga tggcgtagtt cgctttgacg
     7501 tagaggatat caaagatttc atgaatacat tcaaaaaaaa ttgaaatttt cttctcgtca
     7561 catatactat aaatagcgaa atgtactgcc ttacttttta aaggagtgag gaagtttgcc
     7621 tacaaagctt tcaaatggga aatataaaac taatctgagg tatccaaaaa aattcagaga
     7681 aatcactgga attgcatcag agaagtatca aaaagtattt ccaactaggc aattagctat
     7741 taaggccgaa agggaaatga aaaagaagat tgaaacagtt ttaagagagg agaatgcaaa
     7801 ctcactagaa ttgaaaggaa aaataaaatt taaagatttt tatgaatcaa aatggcttcc
     7861 tagatatgaa ctaggtcaaa ctactaggtc aaacagggct ccaagtaatg taacaattag
     7921 taatactaag gatatattcc gattgcacat ccttccaatg tttggtgaat atgcaatgaa
     7981 ttatctgaat tcaaatactg agataatatc agatgaatta acaaagaaat cgaaagaata
     8041 cgcaaatata aaaattatca aaggctatgt tcgctctatg tttgatattg ccgaaatact
     8101 atgttatatc gaattcaacc gaactaccaa aattattcaa tgtattaccg cgcctaagaa
     8161 aatggcttta gaagaaaaaa ggataaggga agggaatcaa gcattatcat cagaagagct
     8221 gactgattgg atagaagcag taaacaatga cttaaataat cacttgctta cacttcacga
     8281 ttacacactt ttcatgctta ccttatatct aggtgataga aaaagtgaga cttatgctct
     8341 gcaatggaaa tatattgatt ttaataaaaa tacggttcgt ctcaaatatg ctttagataa
     8401 atatcaaaga aagacacata caaaaggttg gaaagacacc gttattcagg caccagaagt
     8461 tgtgatgaca ttattaagag aatggaaatc agctcaggct gaacaattgt tgaaactaaa
     8521 aattatacaa acacctgacc agtacctctt tacctattcc aaaccttctg gagaagtaaa
     8581 ttgtcctgtt cacgctgact acttaaacta tcgaatcaat gcaataaaaa aaaggcatcc
     8641 tgaattggct caattatctc cacacaaact tcgtcatacc tatgcaacta tagctaggga
     8701 aggtggagca aatatgaatc aaatctctaa tgctttaacg cactcggata tttctactac
     8761 aaaaatatac gtgaacacac ctgacgtagt tgataaatct gtttttgaag catttcaaaa
     8821 aggattgaat aagtgtgatt aacatgtgtt ctcaaatgtg ttcttattat aaaaaaagac
     8881 actttccaaa taattgaaaa gtgtcctgaa atattgatat aaaaagaata ttaacgtttt
     8941 gagaattgtg aagctttacg agctttttta agacctggtt ttttacgttc gaccatacgt
     9001 gcgtcacgag taagtaagcc agcacgttta agagctaaac ggaagtcagg atcaacttct
     9061 agcaatgcac gagcgatacc atgacggata gctcctgatt gtccagcgta tcctccgccg
     9121 tttacgttaa caaatacgtc atatgcgcct tttgtttcag taacagcgaa aggttgattg
     9181 attacttcac gcaagtcagc atgtggaatg tattcttcaa catctttttt atttacagta
     9241 attttgccag ttcctggtac tagacgtacg cgagcaactg cgtttttacg acggcctgtg
     9301 ccgatatatt gaacttgtgc caatgaaatt ccctcctatt agattaagtt tgtgatgtct
     9361 aaaacttctg gttgttgtgc tgcatgtgga tgttctgctc caccgtatac gttcaatttt
     9421 gtaaattgtt tgcggcctaa agtgttttta ggaagcatac cttttacaga agtttcgatc
     9481 aaacgacgag aatttttagc acgcaattcg ccagcagtga ttgatttcaa tcctcctggg
     9541 tagttgctgt gacggtaata aattttgtca gtcgcttttt tacctgttaa tttcactttg
     9601 tctgcattga tgacgatgac gaaatcgcca gtatccaaat gaggtgtgaa agttggttta
     9661 tttttaccac gtagtacaga tgcaacaact gttgaaagac gtcccaatgg aacatcagtt
     9721 gcgtctacta cataccattt acgttctact tcgcctggtt tggccatata tgttgtacgc
     9781 acgttatttt cctccaattt aagttctgat gtttgacata cacttcgagt ttccggggct
     9841 ctttgtgggg caaacaatac catttaacat attacccgct aatctagtca atgtcaacgt
     9901 ttttctgctt gattgaaaaa tttatatgaa actttacttc ttttctttct tttatataaa
     9961 agggtcgttt cctgtcaaaa tgaaaaacaa aaagaggctg tgacaaaagt cttgtcacaa
    10021 ccccttattt tgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc
    10081 cataaaacat aagaagctgt tttttaaaat tgtttcttgt gactcggagc tgaacggcag
    10141 gcttacaacc tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    10201 cggcaaaata tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg
    10261 atttcacaac ctctttttcg gtcaagttca acaacagttt ttacgcgtcg atctctggcg
    10321 ctttgcccaa atgagcttgg atcatccaga tacgtttttc tgtggctgtt ttgaattcga
    10381 taaagatatc ttgtgttgga tcgtcgcctt cttctccaga aacttcgatt cctttttggt
    10441 aaagatctgc tagataacga tagcctgcaa caagtgtttc caaacgttct ggaatagtac
    10501 gatcccagcg gccaatttca tcagggattt ttgtgttatc tgcaaattca cgcaatgtag
    10561 aataaggtga accgtctaat gtgatcaagc gttcggatac ttcatctaac tgagcgttca
    10621 aatcatccat gaactcatcc atcattgggt gcaatgtaag gaaacctggt cctctcatgt
    10681 accaatgtgt ttggtgaaca acaacagaca tttggcttag atctgcaact aattgattta
    10741 atacttcttt ggtttgttgg aatttcatct ttcttgcctc ctaacatttt cttacactta
    10801 aaataatagt agactttcaa tgaaaagtac aacaaaatca tttacctgag aacgattttc
    10861 agttcgcttt tttatccgta aagccaatca tttttcagaa tcagctaatt cttctcgcag
    10921 ttgagcaaat tgctgataag tctgtgctaa atgcttttgt gtcgaaacaa ataatggata
    10981 gattttctga tattttttat gatcagtttc ttgtactcca acttgctctt tcaattctat
    11041 caattcctgt gcttcactga tactttggat ctttccgatg cttctccagc ctaaaatcac
    11101 agctcctaat gcggatgctt ctgtgacagt agggaaaaca agcgggtaac ccagtacatc
    11161 agccatcatc tgacgaaaaa tcggagattg agcaaacccg cctgtagcac ggatctctgt
    11221 gagctgattt tcctggcaaa ctccttttaa aatccgataa aggttgagac agatcccttc
    11281 tagaatcgcc ttcatcattt ctttttctgt atgttgccgc tgtaaaccga taaaactgcc
    11341 aaacgcatct gcgtcccaaa gcggtgctcg ctcaccaagt aaatagggat gaaaaatcag
    11401 accattggca cccgctggca caaaagcgat agccgaaagc agtgcatcat atggattttc
    11461 tcctttttct ttagcagatt gaacgacttc ctgtaaaaat gtatgccctg cccaatcaaa
    11521 gatgccagca ccattgctag tcgctcctcc cactacccaa tgtttctgat ccagaacata
    11581 acaaaacgtc tccatttctg gatggaactg aggctgagta gtaataaaac gaagtgctcc
    11641 gctcgttcct acggtcaaag cggcgatcgt atcaccaaaa gcacctaacc ctagattaga
    11701 aagcggcccg tctgcccctc ccaaaacaaa tgatatatca tttggcaaac ctagttctgc
    11761 tgctgttttt gatggtagac ctgttacttg ataggtggaa ggaaagactt gcggcaattg
    11821 ctctattcga atgttcaagt agtcaagcac ttcttctgcc cattggaatt catggatatc
    11881 aaaatatcca gttcctgaag ctgttccgta gtcgctgatc agttcgccag acaatctata
    11941 aaagatatag tctttgatac cgacaaaata agcagttttt ttgaataatt caggttggct
    12001 gttttgcagc cacttgattt tgttcaatgg tgtcattgga tgaatcggcg tcccagtctg
    12061 ccgataaaat tctttgccaa gcacggtata tttcagtttt tctgcttcgt ccgctgctcg
    12121 gttatctgcc caagtgatca ttcgagataa cggtgttttc tcttcatcca acaatatcag
    12181 gccatgcatc gcagaggaga aggagacaaa ttgaatgatt gtgtctgaag gcaactgttt
    12241 aattacttct tttataacgg tgcagacacc ttgaaagatc aattcctgat cttgttctgc
    12301 catcccttgt gtttcttgga tcaaagggta gcgaaaataa gcggatgctg cttcttgggc
    12361 ttcatctgtg aaagcaactg ctttggtttg tgtggttccg atatctaatc cgattgaaac
    12421 gttcattttt tcacctcaaa ttttatgtat gtacaaaaat ttagtttaag cttgcgtatc
    12481 ttggttttga aaagggggag atggcgtgtt actttatttt atcattggtt gctgtatcgg
    12541 atcattttta tgtttaaccg cccagcggat tccgttaggt cactctatta tttatcctag
    12601 atctcattgt gtcaaatgct gtcgttcgct ttcttggtat gaattgattc cgattctctc
    12661 gatcatcgtc caacgatttc gctgccgcta ttgcaggtgt cggttgccct tctactattt
    12721 gttggcagaa gccttatgtg gtggtctttt tgcctggttc tttacttttt cttccgcaag
    12781 aaatttttcg acgttcatat ggatgctttc ggctttgcta ttttcgttga tggatttatt
    12841 ttacttcatg gtccattctc acacgctatt ctgttcttgg gcaattttat ggattttttg
    12901 gttgcaggcc ggcgtcttcc aatggcaaag tgtgctgtta acgtttatcg taggcggtgt
    12961 gtgccttcgc tatggtcaat cctatctcgg agctggcgat actctgttga tgctttcttg
    13021 gagtggtggc ttgtctctag aagagttgct tcagatcttg tttcttgcta gtctgtttgg
    13081 aatatccgtt ttttctgttt actttatctg tcataaaaaa aagttggaaa agctgccttt
    13141 cattcctttt ttgagcagtg cgttgctgat tgtccttcat cttaggtatt gaaaaaaggt
    13201 tgtgacaaac gacttgtcac aacctctgat atatagtgtt ctcttaccaa atccttgtga
    13261 cttggagcta ggcgacaggt tcacaacctc tccactaaat ggtgttcaat cagctgaccc
    13321 tcagtattag ctcaaaatgc tggttttaca acctctgata tacggtgttc cataagtagc
    13381 tttttcggct tcttatttct tgacgcccca atgtcgacga aggaaacctg cacgtccaga
    13441 accttctcga taaagattgt aatggagctt gtttttcttg taataatcct gatggtaatc
    13501 ttctgcagga taaaattcgc ttcttggttc gatttttgtc acgattggct tatcgaatcg
    13561 cccgctgtct tgcaatgctt gttttgaacg ctctgcaata tttcgttctt cttcatcatt
    13621 atagtagatc actggacgat aagaatcacc gcgatcagcg aactgtccta atgcgtcagt
    13681 cggatcggtt tgctgccaat agatttccac caactgatca taagaaataa cttctggatc
    13741 atattcgatc tctactgctt ctgtatgccc tgttgtgcct gttgttactt gttcataagt
    13801 tggatttggt acatgcccgc cagtataacc ggatgtcaca gaaatgatcc ctggctgctc
    13861 ttcaaatggt ttgaccatac accaaaagca tccgcctgcg aaaactgctt tttttgtcat
    13921 ataaaatccc ccttttttct catcatacct tgatttccaa aaggaggata acgttttgct
    13981 tgtgacagaa aaaacggaaa ctattatata cgtgatacgg cttctgataa atgccaaaac
    14041 gaacgtactt gatcaaaaac ggttaaatca ggaatcacat attcagcaga aggaagattt
    14101 atgttttcac ctattcccaa tacatgcatg ttaccagcca aagctgcttg acaaccagct
    14161 tctgaatctt caatgaccaa gcatgcattt ggcggtacat tcaattgctg tgctcctttg
    14221 agaaaaactt ctggatcagg ttttgctttt gatacttgcg tgccgtcaat aaggacatca
    14281 aaataacttg tcaaattcgt tttttctaaa atgaggcgtg catttttgct tgctgatccc
    14341 aatccgatct tgatgtttgt ttttttcaag tactcaagaa cagctattgc accaggcaaa
    14401 acggatgttt catccatttt ttgaatcgct tgaagatata gattgttttt ttgctcagca
    14461 aaggctgatt tctctttttc tgagaatttc tgttccattt ttccaaatga cagcaatcgc
    14521 tctagtgact cggttctgga tattcctttc agttgctcgt tttctttctc agtgaatgga
    14581 atggacagct ggttagctaa aaccagccac gcttcgtaat gataacgagc tgtgtctaca
    14641 agaacgccat ctaaatcgaa tagtgctgct ttgattttca tcgattacct ccttgaaaat
    14701 gaacgacttg aagtaaagat gcgatgtttt tagctgtgcg atgaagatta tcagtggctt
    14761 gctgtaaagc ttcttgtaaa ggcacagctg caggaaggat cggaaaaacg gccgaaatgc
    14821 caacatctgc tgctttttcg cttcctttac caacacttcc gcaaatagct atcacaggaa
    14881 cgccctctgg tactcttcta gcaatcccaa ctggcgcttt tccttttaga ctttgtccat
    14941 ctagacgtcc ttcgcctacg attacgaggt ctgcttgctg tacccgttta tcgaactcca
    15001 gtaaatccaa tacgtagtcg atgccagaga ctattttccc tgaagcaaat gctaacagtc
    15061 ctgcacccat tccaccacca gcactactgc ccgatgcctc gattacttga ggggcaaatc
    15121 tcatataaaa atcatgcatg gcttggtcag ccactgctaa ttcattttct ttcaagcctt
    15181 tttgtggtcc gaaaacaaaa gaagcgcctt cttttccgca taaaagatta gtaacatcag
    15241 taatgacagt gattttggct ttaatcagct caataggcac gttcgtttta tcaactgtag
    15301 caattttacc tagattttct cccagtgctt ctactttttg attatcttga tcgtaaaaag
    15361 cataacctag accttctgcc atgccgatgc cgccatcgtt ggtactactt ccgccaacgc
    15421 cgatgatgat gtgtcgaaca ccttgttgca acaattgtcg tatcagttct cctactcctc
    15481 gcgtggataa cctaagtggc tgttgttggt ttagtgggac atctttcaat ccgacgattt
    15541 ctgccatttc aaaaatcgca gtttgaccgt ctgaagcgta ctggatttct ttgtttttgc
    15601 caaatgcatt agtaacgact gctgtttgcc gcttcaacct taaactgtct gctaatgatg
    15661 aaaccgttcc ttctcctcca tcgcctatag gtagcagatc atattcagcc tgaacaaaaa
    15721 cttcttgaaa acctgattga atggcttgtg ctgcctctaa ggctgtcaga ctttctttga
    15781 aagaatctgg cgcaatcaga atacgcatta ttttcccctc cgttccaaga tcagttcttt
    15841 ttccagctgg tattggtctc cattgacaaa aataggtaat gtttctttcg tctctttttt
    15901 tattactatt tgtttttttg tcaaaacaaa tagcaacctg tgatttttcc aaataaaagg
    15961 aaaagaaatt ttttcccatg ctgaaggaag tcttggagaa atagaaagag ttccattcgt
    16021 tactgtaata cctgaaaaac caaatataac tgctagccat agtgctccta atgaagcagc
    16081 atgaattccg tcatctgatg aatgcggatc tggtcctaaa tcaatcaggc acgcttcttg
    16141 gaataaatga tacgcccatt ccatttcttg gatacggcaa gcctctatgg cgtgaatcgc
    16201 tttactcaac gatgagtcgt ggattgtttt gttttcatag taatgcaaat tcttagaaac
    16261 gattggttga gaaaaatact caggtaataa gtataacagc atgacaagat ctgcttgctt
    16321 gctgacttgg agttcattta cttcgctacg ggcataatca agtaaaatag actggctgcc
    16381 aggacgttct ttgtaagtgg tcaaatcaat aactggctta ctgagatacg tgtcatcttg
    16441 cggaatcagc tgttctttgt taggcactgg caaatataac ttatctaaaa aattttgaca
    16501 ttctttcaaa aaaagcggat cgccagcttg atatttaaca gctaaggcca cattataatg
    16561 cgccagatag ttcgtatatg cattattgtc gatatattct gtgtattcat ctggaccaat
    16621 gactctgtgt atctctaaat gatcatcgac ttttacagct ctgctcagcc aaaaagctgc
    16681 tgtttcatga atgagcgttt ttccctttgt ttctaaaaac gattgatcct ctgttgcttg
    16741 gacatactct tgaacggcat aagcaaggtc tgcactgata tgatgttctg cggcagctga
    16801 agcaacttct tgtcttgttc cggttttgat attcaatgca gcaaatttgg gtgtctcttc
    16861 ctgaccagtg tgtgcgcttt cccatgggaa aagtgcacct tgatagtgat aatctcttgc
    16921 tttttgccgt gcgccttcta atcgatcata acggtacagc agcaaattct ttgctacttc
    16981 aggaaactga tataaaaaga aaggcaggat aaaaatttcc gtatcccaga aaacatgccc
    17041 tttataacct tcgccagtta gtcccttcgc ccctacacta acacttggat cattgcgggg
    17101 agtcataatc tgtaaatggt aagcagcgaa gtccatcgct aactgatcga aagaagaatc
    17161 gccttggatc gttatccgat gctgatgcca aaaatctgtc caataagaca tcgactgctc
    17221 taaaatttct gtataactaa cgcttttctg ggcttgggca atacttttct taaacgggtc
    17281 tgtttgctga agagaggtag agagcacgat gatcttctcc agcgtaaagg acgtattttt
    17341 tctgatcttt tctgaaaagg aagcggtgat ccggcgattt tttgcttgga atacacctgc
    17401 tttattgaat ttagctgcaa tcccaatatc aattttactt tctgttgtct ggtaggatgc
    17461 actgatcaaa tcgtcaccaa atactcgcag ttctttttca accaactgct gtgtaccaaa
    17521 attggtttgc tgtccatcga tacccgtttt cacatctact tggcagtctt cggtcaacgg
    17581 cttgatgacc actttcaggg ctgcctgatg gagattattt tgatcagcaa cacgacaagt
    17641 aatgacttcc actttttttc cttgagcagt ttcaatgagc gcatagcgtt taagctctcc
    17701 catttctaaa tctagtgccc gctcgtatgc aagaactttc atcgtctcaa gagaaaaatg
    17761 ttcaccgtcg atttttattt cccatctgat gacatctggg agattcacta actcggctga
    17821 ttctgctcct aaaggtcggt tatatacacc agctgcaaac attcccctta cttgattccg
    17881 atagtcttcc tcctggcttc ctctgatacc aaaaaatcca tttccaatgg tcatctgact
    17941 cgcatactta ttgatatact ctaaatcaaa ctgtttttct tctactttca taaggtaata
    18001 acttctcctg tttctgccga acgatataaa ttttccacca gattctggat gacatatcct
    18061 tgccagccat ctgcaatcat ctgagtctgg tcaccgagcg cccgattcac aaaatgagcc
    18121 atacttagtt catgtgggtc attaactggc gtggtctttt gtaaggaaac cagctttcca
    18181 tctacatctt cgaatagttc gcctggatac agattagcac ctgcctgatc gccacaaaaa
    18241 gccacattca gtttgtcttc tctgatattc aaagcaaacg aagtattcag ttggatgatc
    18301 ttgccgtttt ctaattccag tgtgccaaaa attgcatctt ctactgtgaa tttgcttgga
    18361 tcccatttcc cgaatttccc tgaatttttt ttcgtaccaa ttttttggaa gctgtacgcc
    18421 cgaacgcttt ttactttagg aaatccagta atatacatcg ctgtatcaag catgtgaatg
    18481 ccataatcga tcaaaggccc gcctccttga agttctttgt cgataaacgt tccccagcct
    18541 ggaacaccag accgacgaag cgcattcgct tctacgtaat aaatatcccc gagaacatca
    18601 agatgattct tgatcattgt tgcttcgtca gaaaaacgat gctggaatcc ataagccaaa
    18661 atctgcccgt ttttatcggc tgtctctgcc atctttcttg cttgatcagc tgtcatagct
    18721 ggtggtttct cgcacatcac tgaagcatta gcttcgaggg cagccaatac atgttcgaag
    18781 tgaaagcgat tgggtgtaca gacactcacg atatcgggag aaatctcatt cagcatttgt
    18841 gtaactgatg tatacgcatg agaaaatcct gtacgtcggc aaaagtcttc tgctcgctgg
    18901 ctgactggat caaccacagc ggccaattct aagacatctg atcttttttg ataataagca
    18961 gcatgtactt tttctgctac ttgtccagcg cctatgattg ctactttctt catttatttc
    19021 tccctccttt gcttatttac ttattgttat tctttctcta ttctttctct aaatcattta
    19081 aatgacgcgc tgtttccaga cagtttttca agtattgcaa tgattcctga taaattttca
    19141 cttcattttc tcctcgcaaa cgacattcat ataccatata accttcatac tcattttgaa
    19201 gtagcgtttg aaaatggcga gtaaaatcaa ttgcgccaga tcctggttgg tagcgatgat
    19261 tatctgctaa atggatatga cccacatact ctttttgcgt agtcaatgct tttgaaatat
    19321 catcttcttc aatattcata tgataaaaat cagcgatgat ctgcacatgt ttcaattgat
    19381 tagagtcaat gtaatgacgc gcatccgcta acaaattgat catatggtct tggtatcggt
    19441 tcaaaggctc caaataaata accgtacctg tttcttcggc tactttatct aaataacgaa
    19501 gtgattcgct gactgcttta tagtctcctt ctttgcttcg gggtgaaacc atcggcggta
    19561 atcgataagt gaacattccc caagctgctg ggataacgat tcctttgcct ccaacctcgc
    19621 ttagtgcacg caaaatctca gcaatttgac gtacaccatt aagacgccgt tcttcgataa
    19681 agtcaccgat ccacccatcg tagccgttac aagctgtcac tactgggatc cctgtttctt
    19741 tgattgcctg tttgactgtg tcgatatttt ctattaacaa cttaccgtcg atttcgtatt
    19801 cttcaaatcc acagtctttg ataaatctaa atttttctaa aatgtctctt gggaaaaatg
    19861 cttgatcttg cgtaccgaat ttcattgttc tcctcctcgg acttgaacgc ccattttgat
    19921 actttcttct gggtgctggt ctacataacg catgtaagct tctgcgcttt ctgcaaatgg
    19981 cacaactgga tcaatgattt cttcacaatc caagtaaccg ttcatcaata attcccaaca
    20041 tgtgtcctcg attcgtttac ggttccatct tgggtaatct gggtttggtt cactagatgc
    20101 tcgagcaaag acgatttttg cattattgaa atgtgcttcg cgaccaagat taaacccagc
    20161 tggaaaaggt ttagcaaagg caacataaga aatgatcccg ccatagccga tccctcttaa
    20221 agaagcttgt aatgcggcag cactgccact cgtttcaata actgaatcta ctccacgttt
    20281 atctgtcaat tcctttagac gaaaaccgac atctgctttc ataggatcta gcgtgtaatc
    20341 ggcaccgtat ttttctgcta attctctacg ctgcgctatg ggatcgatcc caacgatggt
    20401 agtagcacct gcttttttag ctaattgaat cgcaatttgc ccgatagcgc ctaaaccgaa
    20461 gattgctaca gcatcacctg ctcgaacatg tgcttcccgg ataccaccta aagcgaattg
    20521 agcaggatcg taacatactg catttttcca ttgatccaac cgatcaagtt tacgtagttt
    20581 ataattatca acaccgtttg taatcagcgt ttcacgaatg ggtccataag tgcagactaa
    20641 ctctcctttt tgatactctg tcaccaaact gcccgtttct tcgatcgtac cgacaatcat
    20701 atttcctaat tgaaattcgc caaataccac accgcgttgg cttccttctt ctcttggcaa
    20761 gaataggcgc cattcttctg aaaattcttc atcgataaat ggacttaatc ctctaaagtc
    20821 gacaatttct gttcctggtt ttggagtagc gaaaattact cggatacgta cttcgttctt
    20881 ttcgatctca cggtcttcgt aatctttgaa tccggctatt cttggttcta atgcaactaa
    20941 ttttttcatc ttttctttcc tctttctttt ctttttatcc ttttacgccg ccttcgacta
    21001 aatcaccttt aataaatcgt tcagataacg catacataat gatgactggt aaagcggtca
    21061 ccaatgaagc ggccatcatt cgtccccaaa tgtagtctgg tgtactgaat aatgtgttca
    21121 aaccgattgg taaagtaaat ttttcggagg tcgacaggaa aatcgaagca aataaataat
    21181 cattccacga aaccataaag caataaacga agacagaaat gattccagaa atagccaatg
    21241 gtataatgat ccgaataatg atctggatcc ggctaagacc atcgatcatt gctgcttctt
    21301 caatatcagt cggaatcgtg tcaaaataac tttttagcat gaatatcgca gtaggaagtg
    21361 tttgaacgac catcgtgata atcagtgctg tattggtatc atacaaacct agaccggaaa
    21421 tgattttaaa taatggaaca acgatcaaaa taccggaaaa catataaacg gtgtaaaagc
    21481 tagcattgat tttcgagcga gctttgaagc gtagttttga caaagcgtat gccccgctga
    21541 tcccaatcac tactgcagtt acggaggcta tcaaggagat cactaaacta tttttgaaat
    21601 acatgatgaa tggaaaaatc tcagcattga aaatatcctg ataatgttca aaagtccatt
    21661 ctttcggaaa aatagtttgt gtcgttgcaa ttgcttcttg tgaatccttg aatgatgtca
    21721 tcagcatgat caaaaatgga aacaaattga cagctaatag caacaaaatc gtcgcgtaaa
    21781 ataagatttt cctgctcttt ttatttttca tttcaagtcc accttctttc gtgagagcaa
    21841 gacgaccgct gcaataatga tgaacaatag aatcgatata gctgctgctt ttccaagatc
    21901 attgaacgca aaggctgtct gatacaaata aacgcctaag atattgactt tattcgtcag
    21961 tagatagaca tctgtaaaca tatagaacat ccagatagat cgtaaaacaa tcacagttgc
    22021 tagtactggt ttgattgccg gcagtgtcac gatcttgaac ttctgccaag catttgcacc
    22081 gtccatttca gctgcttcat aaagcgtttt gtcgactgtt tgcaaaatag ccaaaaatga
    22141 aataaacgca tacggatagt atctccagat agcaaacaaa acgaccataa agaagctagc
    22201 aactggctgg tcgaaccaca gcggtgcttc gttgaaaagc tgcaacctat ctactaacag
    22261 atcattgacg attccataac tattattgaa catatatttc caagcaaaaa tcaaagaaat
    22321 agatggcgta acataactta ataaaatcag tgaacgcacc gttttcctaa atctgaactt
    22381 gcggttaagc aaaatagcta cggctaatcc taaagctgta ctgcctgcta caactaagaa
    22441 tgtgtaagca actgttaaac ctaaagaacg ataaaattca ctatcactta aaatttcaag
    22501 ataattgctt aatccgataa aaacggcgtc tgagttagga ttcagcggca tatcgaaaaa
    22561 actgatctga atattggaaa gcattggaat cagcactaaa acagctaata aaagcatgct
    22621 tggtgctaaa agtcccatgg ctaattttaa gtcagaatgt ttccttggac gattcattga
    22681 aacctccccc tgataatctg ccaatagcta tctacttttt tcgaacacca ttggcagaac
    22741 ttcttgttat ttcgtatttt tttgtgcgtc agctaaagct tgatcgacat cttcgtttcc
    22801 gacagttact tggttcactg cttttccgat cacacctgag cttgtgatat ctcccatttt
    22861 cgtgaaattc ttttccccga ctagtccaaa gacttgtact tcatcaaaag cactagcaat
    22921 ctcagatggc agctcgccaa aagacttgat gacttcattt tctttgtaac catccagttc
    22981 gactacttgt ttattcactg gttgagcacc accaggagac atcaagaccc acttttccat
    23041 gtttttcggt tgagataaga aagctgtgaa tgcttcagca gcctcttttt gtttctcgtc
    23101 tagaccagaa gaaatcgtca atccagatac tgtgccatag acagcttgat ctttttgtgt
    23161 tgggatagca aaaccgatat tttctgagtt ccccgcttca taaacagaag gcaaaatgta
    23221 agttgaatag attgccattg gtgctgttcc attcatgaaa gcatctttga tttctgtcac
    23281 atcattagag cctggcatcg tgtattggga taagttttga taaaacgaca atgcttctct
    23341 catcttttcc gtatcgatcg ttacctcgcc ttcatcatcc aaaacatttg ctccatttga
    23401 caaagcaaat tgagagaacg cttgctcact cattgttcct tcagcagtag gcatagcaat
    23461 tccatattgt ttattctctt tatctgtgaa atgttgagca atttttaaaa tatcatccca
    23521 attttctggt tctgaaaaac cagcttctga taatttttct ttgtcatacc aaataccttg
    23581 aacccagccg ctaattggtg cagcaatata gctttttcca tcttctgaac gaacaaggtt
    23641 tttggctccg tcataataat tatcttctcc agcttcctcg ataacgtttt ggactgccgt
    23701 ctgatcaatc agttcatctt tatccatcac tttagcaaaa tcctgactga cttccattac
    23761 ttctggtaat ttaccggaac gagctaacgt aacgattttt gtattgaacg ctgattcttc
    23821 taccggcact tgcttgattt tgatgtctgg attttctttt tcaaaatcag caaccagttc
    23881 attgatcaca cttaatcgtt cctgttcgac agaagagtgc atgaattgaa tcgttgtctg
    23941 ttgtgattcg ttagagcttt gactttcacc acaagcagta agaagaaatc cagtagctag
    24001 cagacaggta gtaacaataa tttttttcat ttttttccac ctcttatttt attattttca
    24061 gccacatata ttcatacggc tggaatatca actttcgttc ttgactgcaa tggataattt
    24121 gtccagaaaa caggtctagg taatctcctt ttggtaaaag ataacctgct gattgattgg
    24181 aaaaattatg gataactaag actttctctt ccttcgcttc gcgaataaaa ctgataaaag
    24241 aatctgctga gtccagtaag taaatcaaac tattcggatg gaaggctttt tctttttttc
    24301 gaatagatag catctgggtc aattcaccat atactgctgt tcttaatgga tctgtctgca
    24361 actctttctc aatatctgct aacaaaaatt tttgccgatt gatttttcga ttctctcctg
    24421 ttttttctac gcctttgtaa tcatttcgac ttcccagcac actttgaata taaattgccg
    24481 gcacgcctgg taacgttacc aaaaatgaat gagcagctaa aaagcgtttg cttctccatt
    24541 ccgtcgtatc tgttggagaa cttaacgcat ctagatacgt agcgtttatc tcatatggac
    24601 tggtcgttcc atctgggttt ttcttgtagt ttactcgggc accctctttc ttcagctgtt
    24661 ccactaattc aaggatcgtt ttttccggca gaatacctcg taccggattc aatccgatac
    24721 cgtcatggga ggctaaaaaa ttaaaaaaag ttgttttttt cgatgcgaaa tcgatggttt
    24781 ttaaccattc tttcaactta cttgtattac ccgaacgaat cgcttgaagg gtcaaaggtg
    24841 gtagcgaaaa ctgatagacc atctgtgctt cattctcgcc attcccaaaa taactgatat
    24901 tatctttgtg tggcacattt gtctcagtga tcaataccgt tcctggagct gcttcatcag
    24961 cgattgcacg gaataattgg atgattttat gcgtttctgg caggtgtata caagaagttc
    25021 ctagttcttt ccataaaaat cccacagcgt ccaagcgaat aaatgaagcg cctttttcta
    25081 agtaaaacaa aagaatatcg atcattttca ataaaacttc tggattttta aaattcaagt
    25141 ctacttgatc ttcgctaaaa gtcgtccata cgtgtttttc tttcccgttt ttgtcaatga
    25201 acgttgtaag taacgggctg gtccttggtc tagtcacgct gcttgtatct gtatccagtg
    25261 gcaaatcaat aaaaaaatct ttgtacgtat cattcaagtt taaatattcc tgaaaccaag
    25321 tactttcggc agacatatga ttgcagacaa agtcgaacat taaccgtgtt tcgcttctca
    25381 tttcttctat atcttcccat gtgcccaaac ggtcgttgat tgcctgataa tttacaacgg
    25441 agaaaccatc atcagatgta tatgggaaaa atggcaagaa atgtacaact ctaaatgtat
    25501 ctgccaaata cttttgataa aatttcttaa atgtacgcaa agttggttgc tgctcttcaa
    25561 aaaattgatc cccataagta atcaagaaaa tatcttcttc gtcccaatgc agtttgcctg
    25621 cctggccttt cttctgctga taaacactga ctttttgttc aatcatagag gcggttttct
    25681 cagacaaatc tgagtcgcga taaatcgctt gtaaaagttt tgtatacatc ggattcccct
    25741 ttcttttttg ggagcgttcc caacgatttg atcatattat ggaaaacgct tactgtcaat
    25801 agcatttttt taaataattt tgatatacac aaaaatacaa ataagaaagc ttatgtatca
    25861 ataattaata gcttcttaaa aaaatatggt ctctcatgat catgcaagct attcctaaat
    25921 tttcaaaatc atagacaaaa aagaatgtct attgtactat ggaattattc taaaacgctc
    25981 ccaaaaagga ggtgcatata aaattgaaac ctactattta tgacattgcg aaaaaagcta
    26041 atgtctcaaa atcaactgtc tccagagtat taaataacca gccaaatatc tctgataaag
    26101 caaaagcaag tgtacttcaa gcaatcaaag aattgaatta tgaaccgagc aaaattgcaa
    26161 gaggactgtc aggaaatggt tttgacgcga ttctagcatt atctcatcgt acttctcata
    26221 cgacctatgg caatcctttt ttttcagatg ttattcaatc catttctgcc gtttctgaac
    26281 aaaataactt cgacttgatt ctacaaacgg caaaaacacc agaagaagag ctagaaaaat
    26341 gcgcgaaaaa gatccaagaa aaaatgatcc gaggaattat tattttaagc tctacgatgg
    26401 atgaaacact tctttttgaa ttggatcaat acaatattcc tattgtggta atcggaaaag
    26461 tcggaaacga ctacacgaac atctactcag tggacacgga taatttccag gatagttacg
    26521 atttggtgga aacactgatc aagaatggac accgtcatat cggctgtctc catgctccga
    26581 cagaagttta tgtatccaat gatcgtctca atggttaccg tcaatgctta tttgatcacc
    26641 acctagatat ccgtaatgaa tggattatag acggcggctt tcgcttagaa gatagcatgg
    26701 cagcaatgaa tgatttaatg aagctacgag aaagaccaac tgcgattttt tctaccgatg
    26761 cattaaaatc attgagtatt tacaaagtgg cagaacaatc tggaaagcag atacctgatg
    26821 atctttcgat cgcaggattc agtgatccct tattgtcctc cctattttcg ccacagttga
    26881 ctcggataga cattccgaca aatcagttag gaatcaaagc cacagaactt ttattccaac
    26941 taatccacca ccaataccct gaagaaaaaa gaatcattct ttcaaccgag attactctca
    27001 cagattcgat ccaacaacaa gaaatgtaga acagaattat aaaagaacag aaacactgag
    27061 gataagtcag ttactctgtt cttttttcaa tcactcatcc ctcgttaccc cctcccggta
    27121 ttttagcgaa tttttcttat tttccatctg ttttagatag ctgtggataa ctataatgtt
    27181 tgtgttaaac tattttttcc acagggaaga aaaaactatt tttaaccacg ttgtgtataa
    27241 gtttttatag ttcgtttttt taaacacatt tacccttatt tcaatccatg ttattt
//
LOCUS       DADXTH010000034        27214 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.034, whole genome shotgun sequence.
ACCESSION   DADXTH010000034 DADXTH010000000
VERSION     DADXTH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.034"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            161..976
                     /gene="thiD"
                     /locus_tag="IWI97_001404"
     CDS             161..976
                     /gene="thiD"
                     /locus_tag="IWI97_001404"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBC2462914.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1181..2632)
                     /gene="cls"
                     /locus_tag="IWI97_001405"
     CDS             complement(1181..2632)
                     /gene="cls"
                     /locus_tag="IWI97_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBC2462915.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            2974..5640
                     /locus_tag="IWI97_001406"
     CDS             2974..5640
                     /locus_tag="IWI97_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBC2462916.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6004..6756
                     /locus_tag="IWI97_001407"
     CDS             6004..6756
                     /locus_tag="IWI97_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2462917.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6778..7932)
                     /locus_tag="IWI97_001408"
     CDS             complement(6778..7932)
                     /locus_tag="IWI97_001408"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBC2462918.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8117..10558
                     /locus_tag="IWI97_001409"
     CDS             8117..10558
                     /locus_tag="IWI97_001409"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBC2462919.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(10888..11592)
                     /locus_tag="IWI97_001410"
     CDS             complement(10888..11592)
                     /locus_tag="IWI97_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462920.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(11779..12024)
                     /locus_tag="IWI97_001411"
     CDS             complement(11779..12024)
                     /locus_tag="IWI97_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462921.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12041..12892)
                     /locus_tag="IWI97_001412"
     CDS             complement(12041..12892)
                     /locus_tag="IWI97_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBC2462922.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13058..14062
                     /locus_tag="IWI97_001413"
     CDS             13058..14062
                     /locus_tag="IWI97_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBC2462923.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14303..14659)
                     /locus_tag="IWI97_001414"
     CDS             complement(14303..14659)
                     /locus_tag="IWI97_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBC2462924.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15027..16214
                     /gene="tuf"
                     /locus_tag="IWI97_001415"
     CDS             15027..16214
                     /gene="tuf"
                     /locus_tag="IWI97_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBC2462925.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16402..18993)
                     /gene="mprF"
                     /locus_tag="IWI97_001416"
     CDS             complement(16402..18993)
                     /gene="mprF"
                     /locus_tag="IWI97_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBC2462926.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19019..19780)
                     /locus_tag="IWI97_001417"
     CDS             complement(19019..19780)
                     /locus_tag="IWI97_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2462927.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            19947..20579
                     /locus_tag="IWI97_001418"
     CDS             19947..20579
                     /locus_tag="IWI97_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBC2462928.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            20638..21498
                     /locus_tag="IWI97_001419"
     CDS             20638..21498
                     /locus_tag="IWI97_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBC2462929.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            21683..23998
                     /locus_tag="IWI97_001420"
     CDS             21683..23998
                     /locus_tag="IWI97_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBC2462930.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24180..26021
                     /locus_tag="IWI97_001421"
     CDS             24180..26021
                     /locus_tag="IWI97_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBC2462931.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26045..27154
                     /gene="rpoD"
                     /locus_tag="IWI97_001422"
     CDS             26045..27154
                     /gene="rpoD"
                     /locus_tag="IWI97_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBC2462932.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8813 a         4829 c         5700 g         7872 t
ORIGIN      
        1 gacaactatt ttctgattta ttacaaatgt tttttcaaat aaatttgatt accaagaaaa
       61 ggtagtggta tatttactga gggatacttt gaaaaacata tgatagaaac agtaacagca
      121 ttatgaaaaa tctttttata aaaatggagg aatcgatttg atggaagatc agtatgtaca
      181 agcaatgacg atcgcaggat cagatagcga tggaagcgca gggatgcaag cagatatgca
      241 tacattcttt acaagaaatg tttacggtgt ttctgtcatg accgcatgtg ttgcaggtaa
      301 ttcttacgga atcggagcaa gtgtgacatt gccaacagat tttatcgaca aagaatttga
      361 actgattgcg aaggactttc aaatacgtgc agctaaaaca ggtatgctag cagattcaaa
      421 gttgattgag acagttgtaa aaaattacca aaaatatgat tttggaccat tagtcgtgga
      481 tcccgtaatc gtaacgaaac atggaaatct tttactagaa gaatctgctt tgcaaagctt
      541 gaaagaaaag cttgtccctt tggccgaagt actgacacct aatttttatg aagcagaaaa
      601 gctgactgga cggacattag cagctgaaga agattttgtg caagcagcaa aagaactgca
      661 agatatgggt gcaaaaaacg ttatgatcaa aggacatcat gctaaagatg cgcagcaaga
      721 aacagtcaat gactacgtat tgctcgaatc aggaaaatca ttttggttaa atgcaccatt
      781 ttatcctacc gaccggatca acggaactgg cgattcgcta tctgcatgta ttgcagctga
      841 gttagcgaaa ggaaaaacag ttgaacaagc gatccgattg gcaaaagact atgtaaaccg
      901 tgcaattcgt gaagaaataa atgtagggca caaatatgga cccatcaatc attgggcagc
      961 tggtgaatta gagtaaaaga aagaggtcgt gagcctgccg tccagctccg agtcacaagg
     1021 aacaagttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt
     1081 tggcaggaga acaccgtttg ttcggaagaa gggactgtga caagattttt gtcacagtcc
     1141 ctttaatctt agtatatttt tctctacttg aataaaaaaa ttataggatt ggagaaaata
     1201 atcgagaaat ttgttgcttg aataatagcc agttagacat ttccttgact gtttcagttg
     1261 tcatggatga acaatgtgtc tcatcttctt tgaactgagt agcaagttct tctaaaaact
     1321 caggatcgta aatgacagca cttgtttcaa aattcaatcg gtagctacga atatcttgat
     1381 ttgctgaacc taccatgcag atttcatcat ccattatcaa tgtttttgca tggaggaagc
     1441 cgttctcata gacaaggatt tgtacatttt ccttgatcag ctgccgagca taatattgtg
     1501 ttgctcgata aataaaagga tgatccggtt tgttgggaat catgattttc acatctacac
     1561 cagaggcagt tgcgattttt aaagcagcaa tgacactttc atcaggaaca aggtaaggtg
     1621 tctgtatcca aacacgtttt ttagcagaag taatcaattt gataaatgaa agcttgattt
     1681 gttccctttc gttattcggt ccgcttgaaa caagctggat ggatgtatca gcaagcttag
     1741 attcatctcg atctgctttt ttaaagaaat aattcaaatg atagccgacc tttttttctt
     1801 caggtaccga gacgttccag tctgttaaga agcgaagctg gagcaaagaa gaagcggcac
     1861 cgaaaatccg tatatgcgta tcgcgccaat agccgaattt ttttgttgta tttacatatt
     1921 gattggcaat attgaaacca ccggtataac taatctttcc atcgatcaca acgatttttc
     1981 ggtgatcatg gtaattcaat cggaaacgaa gtaatgcctt ttgtgaggta acaaatgtat
     2041 ggacatgtcc accatttttg attagacgat tgaaatcttt tgcttttgta ccatgagagc
     2101 caaatgcatc atataatatt cgaacttcca cgccttctgc ggctttttct tctaaaacat
     2161 gtaagacttg ctggccgata ttatctgtca caaatgcgta atattcgata tggatcgaat
     2221 gtttggcttt ttttagatct tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa
     2281 gagtgacaga attcattctt gtcagcggca tacgatttaa tgaagagaag aagtcgacaa
     2341 attgctgttt gtctttatcg cccatgtcag gatcgtaatg ttcaatggta tctcctctaa
     2401 aagactgaaa gttttctaat tctttcaagt cactttgtcg gagatagaat tttttcttat
     2461 ccgttaatcc acgtccgaaa aataaatata atacaaagcc caccccagga agggcaaaca
     2521 atactaagag ccatgcccaa atggctgcta catctcgggg tttgatcaaa atagccacca
     2581 aagcaataag tgcatttaat agataaaggg cggttataat actagatacc acaaaaatgt
     2641 tcctccttac atacaataac attagtatag catgatagaa gaaagaagcc taccaaggtg
     2701 aatatacttt tcactccttt catgaaaatg taggtaaatc gggctaaagt agttgctttt
     2761 tgtaggttaa taaggtagga tattagtaca gttgaatatc tccttagggg agtaaccggc
     2821 aacgtttgtt gcgataatat caacagtaag ttaaccaaat ggttttctgg tattgtatga
     2881 aatggtgaga cctaagacaa atgcgtattt gtcttaggtc tctttttttt tcagacagga
     2941 ctagggccaa gatcaaaaaa tgggggatga aatttggaaa acaaggaaca agcacaacaa
     3001 gcggtctata aagaaacgat cgactcgttg atgcagcaaa cgcagagctc aaccgatgga
     3061 ttgagcacca cgcaagcgaa ggaaaaatta gaaagagatg gcttgaacca gctaaaagaa
     3121 aagccgaaga agccaacttg gaaattgttc ttagaaactt tcaaagatgc aatggttatc
     3181 gtattgctga ttgtcgcact gattcagatg gtcatgggtg actacatcga attcttagtt
     3241 atttttgcag tattgatgtt gaattctata atcagtgttg tccaaacaaa aaaagcagaa
     3301 ggttctttag aagcgttgaa aaacctttca gcgcccgatg ctcgagtgat tcgtgatggt
     3361 aaggaaaaaa cgattccggc aaacgaactt gtggtaggag atatcgtgtt gttagacgca
     3421 ggcgattatg tacctgctga tggacgttta ctagatgcgg gatcattaaa agtagatgaa
     3481 ggtatgctga caggagagtc gacttctgca gaaaaagaag taacggatat cacaagtacg
     3541 gtaccaatcg gagatcgtac aaatatggta ttcagtggaa cgatcgtaac ttacggacgc
     3601 ggtaaattct tagtgacttc aactgggaat aatacggaga tcggacaagt tgctggcctt
     3661 ttggaatcaa caactgagca agtcactccg ttacaacgag gtctggataa attcagtaaa
     3721 aaactaagtc tggctatttt ggcattgtct ttattgattt taggtatcca actgtttcgt
     3781 atttatcttg gagaaggtac aggagatatg actgcagata tcgtcagcgc agtgatgttt
     3841 gccgtagccg tagccgtagc tgctattcca gaagcattac agtctatcgt aacaatcgtg
     3901 ttatctttgg gaacgaataa aatggcaaaa cgtcatgcca ttattcggaa gcttccagca
     3961 gttgaaacac ttggttcgac aagtatcatc tgtacagata agactggtac attgacacaa
     4021 aacaaaatga cagttgttga ctacttttta ggtaatggcg atactgggga tttcacaaat
     4081 gacccttcaa aatggacagc tgacgaacgt cgcttgatcg aaatcagtgt tttagcgaat
     4141 gatgcatcga tcagtgaaga cggtactaaa ttgggcgacc ctacggaagt agcgtttatc
     4201 gactttagtg aaaaattaaa ccagccttat caggaaatcc gaaacaacta cccaagacaa
     4261 gcagaacttc ctttcgattc tgatcgaaaa ttaatgtcta ctggacacac gatcgataac
     4321 gaaaattact tgtttgtcaa aggcggacca gatatcgtct tccaacgcag taaaaaagta
     4381 ttgctaaatg gtgaagtcgt tccgatgacg gatgaattga tgaaaaaatt ccaagagcaa
     4441 aatgaagctt tttcaacacg cgcattacgt gtactagctt ttgcttaccg tccacttgat
     4501 aattttgatt tatcatttga agatgaagac gatttgatcc ttgttggttt gcttgctatg
     4561 atcgatcctc cacgtgctga agtgactcag gcggtaacag aagcaagaag tgcaggaatc
     4621 aaaacaatca tgattactgg tgaccacaaa acaacagctg ttgctatcgc aaaacaaatc
     4681 gggatttttg aagaaggaaa cctggctttg accgggacgg aactagatga attgacagaa
     4741 gatcaactaa acgaagtttt agaaaaagtc actgtttatg cacgtgtttc acctgagaat
     4801 aaaattcgta tcgttcgtgc ttggcaaaat aaaggtcata tctcagctat gacaggtgat
     4861 ggtgtaaatg acgcaccagc attgaaacaa gcagacatcg gaattgctat gggaacgggt
     4921 acagatgtgg caaaagatgc agcagcgatg gtattgacgg atgataactt tgcttctatt
     4981 gtcagcgcag tagaagtagg acgtaatgtg tttgataata tcaagaaagc tatttcttat
     5041 ctatttgcag gaaatttagg tgcgatcatt gcgatcatct ttgccttagt tgctgactgg
     5101 tccaatccat ttacagcatt acaactgttg ttcatcaatt tagtcaatga ttcactgcca
     5161 gcaattgcat taggtacaga aaaagctgaa cctgctacga tgaaccgccc gccaagagat
     5221 ccaaattcag gaatctttac ggacaaaaca ttgatttctg taacgtatcg tggtgtgctg
     5281 attgggattg ctgttatctt tgctcaatgg cttggcatgc agcattcacc tgaattcggt
     5341 gtagccatgt cattttcaac attgatttgg tgtcggacat tgcaaacatt cccagcacgt
     5401 tcaaacacac aaacagctat ccaagcaggg ttcttctcta acagaagtgt cttactagct
     5461 gtagttgtat gtagcgcgct ttactgctta acaatgatcc caggactgaa agaagtcttc
     5521 tctatcccat cagcatttgg attgacagaa gtctttacat gtatgggaat cgcacttgtg
     5581 acaattgttt tgatggaaat cactaaattg atcattgtac gagtacaagg aaataaataa
     5641 aacgaatcaa cgaggttgtg agcctgccgt ttaattctga gtcacaaggt gcaatttcaa
     5701 caaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaagagtt ggcaggagaa
     5761 caccgtttaa ggaagaagtt gtaaaatcag cattttgagc taatactgag ggtcatctga
     5821 ttggacactg tttattcgga aagagggact ttgacacgac tttggtcaca gtcccttttt
     5881 ttatttatga tagacggaaa gccaatcatt ttattattca tcaaaagaga tagaatagat
     5941 aaaagaggag aataatttat tccgaaaagt gaaaacatat aacaaaaaat tagaggaaat
     6001 caaatgaatg aaaaagaatg ggatgaattt gctgaagaat attatgaaat ccagcaagaa
     6061 tcgcaagtaa ctattgctga agatgtaaaa aattatctga aaacggagaa agtcctgcct
     6121 gctgaaacag cagcagacgt tgctggagga tctggcagat ttctttcctt agcaaaagaa
     6181 attaatcgct atgatttgat cgatttttct tcaggaatgc tagaaaatgc aaaaaaagaa
     6241 gcagaaaaaa atgagttgcc aaatgtttcc tttatcaaac agtcgttaga agaatttatc
     6301 caatcaaatg agacatatga aatggttttt acagcagcaa atccagcatt ggtacatctt
     6361 attcaattag aaaaattgat gaaaaagtca agcaaatggt gcgtgataat cagggtagtg
     6421 gaatcaatcg atgatgtttt ttatcctatc gaaaagcaac taggaatgat agaagaagat
     6481 cctaatacat cgccagttgt aatggatcaa tttgaaacat acttaattga tgaaggattc
     6541 aaagtgaaga aaaaagattt tacttatttt acacaggaat caattactga agaattgatg
     6601 aagctatact atgacgcgta cgcaaaagat cctgttgtga ccaggtacat tcgcaaacta
     6661 ttcagtgaaa aacgagaaat ccttagtacg actaagctga cctatagact gctcctttgg
     6721 tccgcagata aaaaaagaac ctttcacgac tcgtgaaggg ttctttttat gattttatta
     6781 tattttgtag taacgaatat cattttcgat ctttgtaatc gaaaaaggta catctgaagt
     6841 atattctgca cattctccgt gttcaggaag cgtctcactg aaaatcgttt catattcgtc
     6901 aacagtgatc cgtgttcgag aatctagcat ctcttgatga gattgtgcga agagatattc
     6961 ttgatagttt tcttccagat aacctgtaaa aaattcactt actgcaccag aaccgtagct
     7021 gaatagaccg atgcgatctc ctggctgtag tgatttcgaa ttttccagta gagaagttag
     7081 ccccaggtat aatgaaccag tgtaaagatt accgattcgt cggctgtaac ggatgctttc
     7141 ttcatagcga gccataagac gttcctgatt ttcttcgtct gtttggtcta atacgctttg
     7201 caatgccttt tttcccatct tagtatacgg aatatggaaa gcaatcgcct gataatcttc
     7261 gagtcctcga cccgacaatt ctttatgtcg attccaaact ttttggaatg attcgatata
     7321 cgtagaatta gataaaggac catcaacaac aggaaattcg ctataatctg gacgccagaa
     7381 atcatagata tcttctgtca gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt
     7441 ttgagtgatc atcatcgcaa cagcaccgac accttgcgtc acttcaccac cgcttgccaa
     7501 gccgtaacga gcaatatcac ttgctatgac tagtactttt cgttctggat gatttttgac
     7561 atattctttc gccatgtgca aagctgcggt gccaccgtag caagcctctt ttacttcaaa
     7621 agaacgagca aaagattgga ttttcagtaa atggtggata attaccgcac tcgcttttga
     7681 atgatcaatc ccggattctg ttccgacgat gaccatgtcg attagctcac ggtctttttc
     7741 tgttacgatt ttgctggcag catttgctcc tagtgtaatg atgtcttcat ttgcacgatt
     7801 gactgccatt tggtcttgac caatccctat atggtatttt gcaggatcat ccccacggct
     7861 ttctgccaac tctgtcatat ctaaatataa attaggaata aaaaaggaaa gacgatcaat
     7921 ccctattttc atttcaacac ctctttactt atgataaaaa acttacttta ctaaggtatc
     7981 ataaaaagat taaaagaaat ataaaaaaga gaatatttaa ggattgttaa aatttaatct
     8041 tagaaaaaaa caactggaac aagagctatt tgtttttaca gcaaagaatg gtatattttt
     8101 gtaggaggtg gcttacttga aagaagtcgt tatgatagat gctgcaagaa cgccgatagg
     8161 aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa ttaggcacac ttgtgactaa
     8221 aggattatta gataaaacaa aactgaaaaa agataaaatc gatcaagtaa tattcggaaa
     8281 tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag attgctttga acagcggtct
     8341 ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt
     8401 gatcttagca cgccagctga tccaattagg tgaagcagaa cttgtgatag ccggcggaac
     8461 agagagtatg tctcaagcac ctatgctgaa accgtatcag tcagaaacaa atgaatatgg
     8521 tgaaccaatt tccagtatgg tcaacgacgg attgactgac gcattttcaa atgcacatat
     8581 gggattaacc gcagagaagg ttgcaacaca attttctgtg agcagagaag aacaggatcg
     8641 ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc gaagccggtg tattttctga
     8701 ggagatcatc ccagtcaaga tttctgatga agacgtgtta tctgaggatg aagcagttcg
     8761 tggaaatagt acattggaaa aactgggcac gttacgtaca gtattctcag aagaaggaac
     8821 tgtaacagca ggaaatgctt cgccattgaa tgacggtgcc tctgtggtga tccttgcatc
     8881 caaagaatac gcagaaaata ataatctgcc ttatttagca accatcaaag aagtagcgga
     8941 ggtcggtatt gacccaagta tcatgggaat cgctccaatc aaagccattc aaaaattgac
     9001 agatagatca ggaatgaatt tatcaacaat cgatcttttt gagatcaatg aagcatttgc
     9061 agcttcttct atcgtagtta gccaagaact gcagttagat gaagaaaagg tcaatatata
     9121 tggaggagca atcgcattag gccatccaat tggtgcaagc ggtgcacgta tcttgactac
     9181 attagcttac ggattgcttc gtgaacagaa gagatacggt attgcttcat tatgtattgg
     9241 tggaggttta ggcttggcag tcttgttaga agcgaatatg gagcaaacac ataaggatgt
     9301 gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg cgcagtcaat taatagaaaa
     9361 aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa caaacacttt ctgaagagct
     9421 ctccgatcac atgatcgaaa accaagtgag tgaagtggaa atcccaatgg gcatcgcgca
     9481 gaattttcaa atcaatggta agaaaaaatg gattccgatg gcaacagagg aaccatctgt
     9541 cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca
     9601 gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa
     9661 tgcagttaat catcgaaaag aagagctgat tctttgtgca aatgaaagtt atccttcaat
     9721 cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg gaattcatgg ggagtttcca
     9781 tgcatactta tctattgatt ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat
     9841 caactcgatt cttgagagtg tggcaaacaa gttacgggag tggttccctg aagaagagat
     9901 tctattcagt attttaagta attttgctac agaatcattg gcttctgcct gttgtgaaat
     9961 cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca
    10021 acaggcaggg gaatatgcga agcttgaccc ttaccgtgca gcaacgcaca acaaagggat
    10081 catgaatggt atagaagctg tagtggcagc tacaggaaat gatacacgtg cggttagcgc
    10141 ttcgattcac gcctacgcgg caagaaatgg attataccaa ggattaacag actggcagat
    10201 caaaggggat aaactagttg gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg
    10261 agcttccaat atcctgccaa aagcaaaagc atctttagct atgttagata ttgattcagc
    10321 caaggaacta gcacaagtaa ttgctgcagt agggttagct caaaacctgg ctgctttgcg
    10381 tgcattagtt acagaaggaa tccaaaaagg ccacatggga cttcaagctc gttcattagc
    10441 gatctcaatt ggggctattg gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc
    10501 agaaaagatg aaccagcaga cagctataca aatattggaa aaaatccggg aaaaataaaa
    10561 aagcaatatc tttagaagaa taaagcaaga tttacagtta ttttatttac tgcaaatctt
    10621 gcttttttct ctatcagaaa atatgtttat tattagaatt ttttatagtt aaggttaggc
    10681 atcttaagaa atcaggcaac tccaagatat cttcaggtag aagagaagtc gcctaacatt
    10741 gtaggatcat aggtgatttg ttggacaggt caagctgata tttgagcctg atgttagaag
    10801 gaatttcttt ttacttgctg tagaaattat tatatgacta aaaaggctgg gattttaaat
    10861 cccagccttt tatgacaaat agaattttta tcgatcagta atcgctgtcg ctgcaaattg
    10921 ttcgatcaaa ggaatcaaag tcaatttgac tttgtatttc cgttcgagtg ctttttcgat
    10981 aacgaactca ttttcagtag tcatagaaat agttgctaat ttacgaggtt catcagcatt
    11041 tttttcatta ataactactt catgttcatt ttgttctgtg taaaatttag gatactcagc
    11101 aacggctttt gaaaaatcag caaaacttaa agcggcattc gtatcagttg agcgtgtatt
    11161 ttcagttgtg tccgcttttg gtttttccgt tttttcttct ggcacactga ctgtttcttc
    11221 tgtaactggt gtcgcaacag cttgcgcagt tggttcatcg tttttcaatc gttgatcatt
    11281 ccagtaagac acgatttcaa taagaagatt gtcacggaat cgttcgaaaa tcccgttttc
    11341 tcctgaacga attctcttta atacagtatt gaagttgtca tcgattggca cgttgatccg
    11401 tgtatcttca tttgttttcc cagctaatcc gatcgttaag atttgacggt ttttgggatc
    11461 aagcatgact tgtaacggca aaccatagcg tcgcaagctc tcttcttcta gagaacggaa
    11521 taattgtcct cttacaccat ccaatgaagg acggaattga tttaataatt tttgtttctg
    11581 ttttttcttc atatgttctc tccttcgctc ataccctttc gattataaga aaaaaacagc
    11641 caaatgaaaa ggagtaatgc aaaaaaactt gcgtatacgc aaattacaac agaaaaattt
    11701 gcgtattttc ccgttttttg aaagaaaaat aaaaaatgga acggattatg tatatataca
    11761 taatccgttc cattctttct attttttcgg tatcaaggca aaaacaggta gttcaggtga
    11821 ataaatgact tcatgttcgt gccagggttt gaccattagc gaatgatcga accgtttttt
    11881 cagttcttcg atgatttgat catgtgtcca gtttcttgca ggaaaatgtt ggattttctc
    11941 tgcatcgatc aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt ccgagagaga
    12001 attcaagcgt tcgattggat ccataaaatc acctttcgac ttaaaattgg gtagtatctg
    12061 ggtcttttgc tttgccgact acaccatcta actgatcgat ttgtttcatt tcttcttctg
    12121 ttaattcaaa atcaaataat tcagtatttt ctttgattct gcttggtgtc actgacttag
    12181 gaagaggaag gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt
    12241 catgtgcttc agcgatttgt ttcatttcag gcacatcgaa gattttacca gtgcctaatg
    12301 gactatatgc ttcgaggatc atatcatgtt tttttgcata ctcaactaca gttggctgta
    12361 attcgcctgg tgcaaggaaa acttgattga ccatcggcat aatttttgct gtttttgcta
    12421 gtgtatctag atggtggggt aagaaattgc tcacaccgat tgctttgatt ttacctgctt
    12481 catacagttc ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa
    12541 aagcaacagg gtttggccag tggatcaaaa acagatctaa ataatctgtt tggagcttct
    12601 tcaatgattc ttcaaaagaa gacatcacaa gttcatagct gtggtttgca ttccataatt
    12661 ttgttgtcaa gaatatctct tctctaggaa ttccactttc cttaatcgct tggccgacac
    12721 tttcttcatt cttgtatcct tgggcagtat cgatatggcg ataacctgca gccagcgctt
    12781 ctttaacaga ggaaacagcg acatccccat caggtgtttg ccatgtgccg aatcctacta
    12841 ccgggatttc gtaaccattt aatagacgat aagtgcttgt taatgaattc atactttcag
    12901 ctcctttttt attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt
    12961 tgtttctact cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat
    13021 tttttttccg taaactaaga cagaagggaa tgagaaaatg gatatctatt taaaaaaagc
    13081 aattctgcat attattgaca gagaaactgg ttctccagtc ttttcacaaa aagagctgga
    13141 tctgacaaaa gaatatattc gtgattttct tcaaaagaag atccaaaaaa tctcttctgc
    13201 tcaaacaaaa actggacaac tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc
    13261 tcaagaaaat tttgtggaag caagtgaagc aatcgttcag cggtggttcg atatctacca
    13321 agaaagtgaa gaagcaccta gtgcagatgt atttgttgtt ttgtatgaat tagatacagt
    13381 gatgtactta tctttattga aagtcaatta tcgtgaggct tacactcact ttgttgaagc
    13441 agacgaagca gggatcgaca accgcttgat tttgaatcga gcaattctag gggcgaaaac
    13501 acaaaaagca gatgaagcat tcgctgtgaa tttgactgat tggacctacg aattaattga
    13561 aaaaaaatat gagttttctg gcaaaaaaga gctttatttt tctggaagag tgatcgaaag
    13621 tgttcctgca ccttctttag aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct
    13681 tggaaaaaaa tttgagacag aagaatttga tatcatggct gatttgaaag aagctgtcta
    13741 tgatacgata gaagaaaaag gtcggttgga tcatgagatg attgcggaaa aagtcttcaa
    13801 ggaaaacatc acggcaaaaa tggcttttaa agaagaagta caagagcaag gatttgtccc
    13861 agaagcacct cctgtaaaag aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa
    13921 attcaagcta tcaaacggga tcgaactgat cgtaccagta gatgtatatc gcaacccaga
    13981 tttgattgaa tttgtgaata atccggatgg tacgatatcc gtgatgatca aaaatgtgga
    14041 tgaagtattg aatcgtttat aaaaatgact agaacattag gtgttcaagt accagtgatc
    14101 agttaaatga attgtgtcaa gagaaggatc ggaaaagacg aatataacaa aaaaagagcg
    14161 tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
    14221 agaagcaact ccttcttatt tcttggagtt aaacgcttct gtcccagcct ctttttatct
    14281 aataaaattt taagaccatt tactaattat cgattttttc tgccgcattt ttaggcactt
    14341 cagaagcctt tatttcttcc cagcttaata cacctttacg aggattgact ttgagtttaa
    14401 gatacgcatt catctttaat ggatgttctc tttcttcgcg aagcgtcacc tgtttttttt
    14461 caccatcttt attataagcc gcttgcttgt aggtataagt cgtgaatttc tctccgttat
    14521 ctgcttgggt agctgacatt tcgccattcg tcgtgatttt tgtataataa gcttcgccgc
    14581 cgtaaaaata atgataagct gcaaaacttc cgccgagaaa tatgcctaaa acgacaagta
    14641 cgccaattaa ttttttcatg tacttcacct ctttccttat tattaacgaa atagaagatt
    14701 tcgtctattg aaagttgttg gcaacagctg aattttaaac ttttcctcat tttgttctta
    14761 ttgcttcaaa agaaagttaa ggactatctt gaagtttttc tcaaaaaaca cattttttta
    14821 tttataaaag gtactttttt cgcgtataag acaagcgagt gctcttagtt gactttgacc
    14881 aatattgacg tataataagc ttagttcgaa tcttacaaaa agtaagtcgg acgtattctt
    14941 tttgatttag tcaaaaaaag actataataa aaaggtgaga gctgaaattt ttcagtgatc
    15001 tatattattt aggaggtttg taaattatgg caaaagaaca ttatgacaga agtaaaccac
    15061 acgttaacat tggtacgatt ggacacgttg accatgggaa aactacttta actgctgcga
    15121 tcactacagt attaggcaaa aaaggcctag caaatcctca agactatgct agtattgatg
    15181 cagctccaga agaacgtgaa cgtgggatta cgatcaatac cgcacacgta gaatatgaaa
    15241 cagaaaaacg ccactacgcg catattgatg caccaggaca cgcggactat gtgaaaaaca
    15301 tgattacagg ggcagcacaa atggatggtg caatcttagt tgtttctgca actgacggtc
    15361 cgatgcctca aacacgtgaa cacattttat tgtcacgcca agttggtgta aaatacctga
    15421 ttgttttctt gaacaaagtt gatttagtcg atgatgaaga attgatcgat ttggtagaaa
    15481 tggaagttcg cgagttattg agcgaatatg gtttcccagg cgatgacact cctgtgatca
    15541 aaggttccgc attaaaagca ttgcaaggcg atccagatgc tgaagctgct attatggaat
    15601 tgatggatac agtagatgaa tatatcccaa caccagaacg tgatacagat aaaccattac
    15661 tattgccagt ggaagacgtc ttctcaatta caggtcgagg aactgttgcc tcaggtcgta
    15721 ttgatcgtgg tgctgttcgt gtcggtgatg aggtagagat cgtagggatc aaacctgaaa
    15781 cacaaaaagc agttgtaaca ggtgtagaaa tgttccgtaa aacgttagat tacggggaag
    15841 ctggggataa cgtaggcgtg ttgttacggg ggatccaacg tgacgatatc gaacgtggac
    15901 aagtgcttgc taaaccaggt tccattactc cacatacaaa attcaaagca gaagtgtacg
    15961 tgttgacaaa agaagaaggt ggacgtcata ctccattctt caacaactat cgtccacaat
    16021 tctacttccg cacaactgat gttacaggaa caatcacatt gccagaagat acagaaatgg
    16081 tcatgcctgg tgacaacgtg acgattgatg ttgacttgat ccatccaatc gctgttgaaa
    16141 acggaacgac attctcaatt cgtgaaggcg gacggacagt gggttcaggt atcgttacag
    16201 agatcgaagc ataagaaacg cgactcgaat attttggtaa tgaaaaatgt ttaaaataaa
    16261 agagcggtcc aaacaggatt tctgtttgga ccgctctttg ttcttcaatt gtataagaag
    16321 agattgtaga agcatgaact ttcgttttac ttcagtctct tttattcatc atgagagtgt
    16381 tcatctatcg gtatgcagtt tttaatactt tcttcgtatc cattttttca aaccacttat
    16441 tctaccttgt gtctgctcaa ctggcgcatt atcaataatc agcaaagcaa tcataacata
    16501 tgcaatccaa ctatctctag aatatagagt ataacgtgag acccagtttg tagcgtactt
    16561 gcttttatat tctcttaatc catggaaaga ataaaaatga gaaccaaact cgtaaaccaa
    16621 gtatgcaatc ctttcctgta aaaaactctt tctggaagtt ccgacatttg aaagaggggc
    16681 catccctaaa ttgaaccatt ggatatgttc ttctttcata taggcaaaca aatgcaagaa
    16741 caagaaatcc atactgccag aaggtgcttt tttaggatca tatcgcatca gatcaatcgt
    16801 tcctacttta ttgttcgtgt aagtaggcat gatcgttgca aaagcgacaa tttcttcttg
    16861 ggtatttttg acaactgcaa taggcgctct ttgtagataa tcttcagaga aaaagcctaa
    16921 agaaaaccct ttttcctttc gtgaacccaa ccaattatca gagatatttt tgaaagtact
    16981 catttgctca gtagaaaatg gtggttgtaa gacatcaaat gtaaatcctt ctcgttcgat
    17041 ccgattcaag acagcacgag tgcccttcat ttttttccct gaagttgtaa aactattcag
    17101 gtctacataa gcttcttccc ccatcttaat gaaatcgtag ccgaattcat gcaaaatcat
    17161 aacaatctct tcgcttgttt cgtagaaaac tggtaaatag catagtcgat ctgtttcttc
    17221 gataaaagct tcgattgctt ctggaaaatc ttctttctta ccagaaggat cacccattac
    17281 aatacattta ttattgaagc aagcgaattg gagaagaaca gttggttcac cgtctttttc
    17341 ataggcaaac atccgcttat cttttaagaa gatcaattga ctatcggagt ttcctccata
    17401 agttgtaagg atcttcaggg ccttttcttc attaaaggct tcgccaatct gtttcttttc
    17461 tccttgaagg aaatgaacaa atagaacgat catgaaacta acagcgatga tggccaacaa
    17521 tcccgaaaac caaatctttt cagaaggaaa taagaaaaag gaaataaaat ggtgacgccg
    17581 atgagggaaa tccggcagat tgtatacccc aataacgatg taaagcaagg acagtgcacc
    17641 aataatgatt ccatcgatcg tacgccactc ccatgaatag atcaattgtt cccgaaataa
    17701 ttcgttttta gaagcaagaa tgatcagaag cagaatagac aagaaaacca ccggtgtgaa
    17761 actaaaatca cttaataaaa cataaagtaa agctagaaca atcaagaaga ttgtaggcaa
    17821 atatgctcgt ttcacacgtg ctgcgatccc tctgcccata atgattaaaa gaaatcccaa
    17881 tagtatacta ggaaattgga taataaaatg aaatttcaat gggttcaaac tatgaagcca
    17941 gcgaaactct gtaaaagctt gcgggatagt ggcagaaagt actagcatga ttccagaaaa
    18001 gtataataag acaaccacga ttttatgcgc aatttctgtt gctaattgtt tagggatacc
    18061 agaataacgc tgatcaaaag tggaaccgat atttttgaag aaaaagacga ttccaatcaa
    18121 aaacggaatg atgtagtaga acaggcgata gagcaagatc cacacaacga ctatttctct
    18181 tggcacacca atagcggaca acccgataat catcattaca tcgaaactcc caatttctcc
    18241 tggaatcata gacacgattc ctatcactga agcagcaatg aataacggaa ttgtttgcca
    18301 cagatcaatc cggatttcca ttaaataacc aacagaaagg aaactaaaca ggacaccggt
    18361 ccattccaac aatgaaacta gcaatagctg tccttgagtt ttaagtgagg taccaccaag
    18421 gaatcctttc tttttgaaag tagtaaataa aaagacgaca ggaaaatata gaccgccgcc
    18481 gatcaaccat atccaatatt gcttcaaaaa aggagcaact ggcgtgaaga ctacaagtaa
    18541 aaaagaaagc agactgtaaa tagaaagtcc agccatcaaa aacaagaaaa ctttcgataa
    18601 tgcttggatc acgtttctgc tctctttctt ttgtccatat agttcagagc gaagccccac
    18661 actaacaaac ccgccgaaac cggcaatatt gttgattgta ttgatcagcc agcttgtctc
    18721 aaacaagtaa cgaggattct gcttttgttc aagcatccga ttcaaaataa tatcataacc
    18781 aatcataggt aacacagaga caagaccaat caacagcata agtccgcttt tccatacagg
    18841 tatagtggca aacgtttctc ctaattgctg gatagataga gttttaccga tggacatcaa
    18901 ttcaccgaca ataatgacaa tgacagaaat caaaaagatt gttttgaaca gtcctaggtg
    18961 gtttttcagc cattgtatgg tattttttaa caaataaatc accttctatt cctaatagtt
    19021 atgtttcaga aagaaattga agaacttctc gattgaagcg ttcaggatct tttctagcta
    19081 attcatgtcc ttgttctttc acaagcacaa aggaagcttt tggtatagtt ttagctatat
    19141 acaaagaatg ttttagcttg attacatctt tttttcccac gatgatcaaa gtgggggagt
    19201 tgattttttt taaatcattt tctgtaagcc cgatgtcatg cagcaaaagt ttgataacta
    19261 aaagattttt gcgaagactt gggcgaaata aagagagtaa ccatacccat gcataatgaa
    19321 tattactgat gactctggca gaaaaacgaa cgcctttaac aagcgtgttt ccagaattta
    19381 aaatcaatcg atgaactttt ttagggaagc tgctggcaaa taccagtgct agatttgctc
    19441 catcactaaa tccaagaaaa tctgcctgat cgattttttc cagcaacatg atcgtattca
    19501 gatcctcggc cattaactga aaattcaaca tagatgcttc gtttgtcgaa cgaccatgtc
    19561 ctcggctatc tactagatac acggtatagt agcgttcgag tacagggacc tgttctgaaa
    19621 aaaagcggcc gctgccgtca ttgccatgca gtaaaaagag agggaagcct tgaccgctct
    19681 tttcatagta aatttcgctc ccatctggca tttttgcata tttcttttct tttttcacat
    19741 aatcacctac tgtgtaaagt ataacgatta tatcagtcat tgggcaattg tttttactga
    19801 aaaacaacga tcattttcgt aagatgacaa aaaatacagg aaaattcatt ttttcttaag
    19861 gcgtacacag aaatcaagga agaaatagcc aagaagataa agaaatagta agataaaaaa
    19921 agatgtcaaa ataactaggt gatgaaatga agcgaaaacg attatctatt atccgtattc
    19981 ttttttacat gatcattctg acggttattg gtgcggtgtt gtttttagga ggaagaactt
    20041 atcgttcggt aaaacgagtg gaaagctatc aaatagaaat aacaacagct gcaaaaaatt
    20101 atcatatgga aaactacgag aatttgatca aggcgataat attgaccgaa tcaaaaggaa
    20161 aagggacaga tctcatgcgg agctcggaaa gtctgcatgg agaagctgag atcattgata
    20221 atccagctga cagtattgat caaggtgtca aatatttggc ggaaatgata gtagaagcaa
    20281 agaaacaagg atgcgattta tggacagctg tccaagcata taattttggg aaagattata
    20341 ttgcctatgt aaaaaatcat ggcggagaaa acacagtaga attagcagaa gcgtattcta
    20401 aagatgtgct gagcccactt ttaggaaatg aggaagggaa acaataccga tattggcgga
    20461 tccagtcctt gctttacaat ggcggctatc tctatcataa tggaggtaac atgttttatc
    20521 ctgatattgt caaaatgaat gaaaaaataa tagagaccta tgaaaaagtc tttaagtaaa
    20581 atagcaggaa atgttcaatc atgaataaaa ttaaatgaaa gaaggagtgc gcaaatagtg
    20641 gaaaaaaggc aaaaaatgga aaatcaagaa gtggggtaca caaccaaaga ctggatgtta
    20701 cggtttgtga agggaatgtt tatcggttca ggatttatct tgccaggagt cagcggtggt
    20761 gcattagcag ctgtctttgg catgtatgag cggctgattt cttttttagc ccaccttaca
    20821 aaaaatttca aagaaaatgt tttgtttttt ctgcctgttg gtctaggcgg agtgacaggc
    20881 atctttctac tttctttttt agtcagtttt ttattaggtg cgtatgaaac aacgatatta
    20941 tggttttttg tcggatgtat cgctggaact gttcctgcgt tatggaaaga agcagggaaa
    21001 aaaggaagag atacttctga tgtgatcatc atggttgtta cttttgttct ggcttatttg
    21061 tttttattat acggcgctca attatttgac gggcaagtcg atcagaattt tggaacatgg
    21121 ctgattgccg gcggattgat tggtttgggt atgatcgtcc caggattgag cccttctaat
    21181 tttttggtgt atatgggatt gtataaagcg atggctgacg gatttaaaga agtacgactt
    21241 gaagtgattg tgccgatcgg aattggtggg attctttgtg ttcttggatt gtcaaaaatc
    21301 atggattaca ttttctcaaa ggcatatagc aagctattcc atttcatttt aggtgtagtt
    21361 tttgcttcaa cgattatgat cattcctacc gattacgcgg gattgggagt tgctggggta
    21421 ctagcttgtc ctgttctttt tatagccggt acagcactag gttggtggat gagccgtttg
    21481 gaggaacaat ataagtaaag atagattgat aataaaaaca gcacacgggt gattcaaaca
    21541 aaaaggttgg catctttttt gtaaaatcat tcgtgtgttt ttctataaat tttataaaaa
    21601 caagttgttt ttaatcttgg aacacgctag acttaaacac gtgattattt ttgaaagttg
    21661 gcggaaaatg gagaaaaaga aaatgaatgg acttacacag caagaggtag ttcagaaaat
    21721 ggcttctggc cagcaaaatg actaccagga agatgcatca aaaagtacaa aacaaatatt
    21781 cagcgataat atattgacgt tattcaattt tctgaacttt ggtattggga tttgtctgtt
    21841 atttgtaggt gcgtattcca acatggcgta tttagcaatt attgtcgtca atatcgttat
    21901 tggaatttat caagaaatac atgcaagaaa tcttgtacgg aaattatcga ttgtttccca
    21961 atcagaagta ctagtgatcc gggacggtaa agaacaatcg atttctacta aagagctggt
    22021 attaggagat ttggtaaaaa tcggagcagg agaacagata ccagccgatt tacaggttat
    22081 atctggacgg gcagaagcaa atgaagctct tttgacaggc gaatctgatt tgattgtcaa
    22141 agaaactgga gctgaattgc tttctggaag ctttttgaca agcggttctg tcatggctga
    22201 agttatccga gtaggtgcag ataattatgc tgtccgcttg actagcgagg caaagactca
    22261 caaagctatt cgttctgaac ttgtagattc gattcgcaaa gtatccaaat tcactagttt
    22321 cgtgattatt cctttgggga tcatactatt tttggaagca ctgttcatcc gcgatgcttc
    22381 tatcaaaatt tctgttattg catctgcagc agctttgtta ggcatgcttc caaagggttt
    22441 ggtattgcta ataagtattg cattaacgac aggggtaacc aaactggcaa aaaaacgaat
    22501 ccttgttcaa gacatgtatt caattgaaac gctggcccat gtcgatacat tatgcttgga
    22561 taaaacaggg accattactg aagggaaaat gagtatccag cgggtagaag cactacatcc
    22621 gctgtacgag aaagccatcc cagaaattat ggggacgtat ctggcagaaa gcagtgataa
    22681 caacatcact atgcgggcta tgagagatta ttttgagctt tctaatcggt atgatgtgac
    22741 aaacaaagtc gcattttctt ctgatcgcaa atggggcgcg atggaatttc cagaactggg
    22801 agtcgtgtac gtaggagcgc ctgaacgttt ggtggaagaa gatcggcttc cggacagtct
    22861 aaaaacagcc caagcgaatg gctatcgcgt acttatgttg gggatcgctc caaatcagca
    22921 attgaacgaa gaaaaaccag catttgttga gccggttgct ctgtttgaaa tcgatgatcc
    22981 aatccgaaaa aacgctcaag aaacactagc ttatcttcat gaggaaggtg tggatctgaa
    23041 agtgatttct ggagacaatc cttttactgt ctccaatatc gctagacgtg caggattacc
    23101 gaattatgat gcgtatgtcg atctttctca aattactgaa gaaatggaag tgcgagaagc
    23161 tgctcatcgc tatacggttt ttggacgagt gagtccgcag caaaagaaat tgctggtaaa
    23221 tgaattgaaa gaaagcggca gaacggttgc aatgacaggc gacggtgtga atgatgtttt
    23281 ggcgttaaga gaagcagatt gcagcattgc tatggcagaa ggtgatggag caacacgtca
    23341 gattgcgaat ctggtattgt tggactctga ttttactaca ttgccagaag tcctgtttga
    23401 aggacgccga gtggtcaaca atgtgacgaa agtctcaggc atctttttta tcaaaacgat
    23461 ttattcattt attttatcga ttatatgtgc cgttacagca attggatttc cgtttatccc
    23521 tatccaaatc acgctgattg atttagcgat tgaagggtat ccttccttct ttttatcttt
    23581 tgaacaagat aaaagaaaaa tcacttaccg ttatttgcct acagcactta ttaatgcttt
    23641 accaaatgcc ttgcttgtag tagtgaatat cattgcggtg tatctacttg gaaaaacatt
    23701 cgcatgggga aatgctgaaa ctactacttt gatgtattac ttgctgattg ggatcagttg
    23761 catggcagtt gtaaaagctt gttatccctt caacccattg cgcatcttcc tagcagtaac
    23821 gacgatcgta gggatatatg tagctgcaat gctgttccat ggaattttgg aagtaaactt
    23881 agggttggga gagagcgtag gatacttctt tgtattcatg ctcgtaaata ttgttcttcg
    23941 agtattgtac tggaaggctg atattcaaaa tcagttactg aaaagagtag caaattgatg
    24001 cttaatgaga tagaaatgtg ttatacttga aaaagcaatg aaatatgata caatcaaaca
    24061 tgtataattg tgtcctttaa aatcgcactg atttcagtta gtgcgatttt ttaccggtta
    24121 ttctcctgaa atatcttctg ttaaacccaa aagatatcaa tagagcaggg ggtgaaaaaa
    24181 tggctcaacg gattcctcaa gaggttatcg aaacagtacg cagtcaaacg aatatcgttg
    24241 aggtgattgg acaatatgtc cagttgaaaa agtctggtaa aaattacttg gggctgtgtc
    24301 cttttcatga agaacgtaca ccgtcttttt ctgttgcaga agacaagcag atttttcatt
    24361 gctttggttg tggtaaaggc ggaaacgtgt ttacttttct tcaagaacta gaaggtctaa
    24421 gttttccaga agcagtggta aaagtggcag acttcgaaca gataccgtta gaagatcaat
    24481 ggcgtcagcg tactatagct gtccaaaatc ctgaatctgc tacggggaaa ctgattgctg
    24541 ctcatgaaaa agcagcggaa atctatcatc acatgctatt gcatacaaaa gctggacagc
    24601 cagctttaga atatttgcaa agtcgtgggt tgtctttaga attaattgaa gaattcaaca
    24661 taggttttgc acctaatgag cgttcttttc ttcagcaagt attcaagaat gaatcaattt
    24721 caaatgaatt gttagaacgc tctggattgt ttgtcacgca tgatgatggc cgtatgtctg
    24781 atcgttttta tcaaagagtg atgtttccac ttcgtaattc tcaaggccag acgatcggtt
    24841 tttcaggacg ctggcttgca cctgaaaaag ggcaggaaaa agatcagccc aaatacttga
    24901 atagtccaga aacagaactg ttcaataaaa gacaagtttt atttaatctt gataaagcaa
    24961 gaagcgatat ccggaaaaac ggagaaattt tgttgttcga agggtttatg gatgtgattg
    25021 ctgcatggca ggctggtatc aaaactggat tagcttctat ggggacaagt ctaacagctc
    25081 aacagatcca acaaatggaa aagctgacga aagaattggt cttttgctat gacggagaca
    25141 acgcaggttt tgaagcaacc aatcgaggga ttgaattgct tcggcaaaac agtcgattag
    25201 cactttctgt cgtagtcgtt ccagaaaagc ttgatcctga tgaatatctc cgtaagtatg
    25261 gagaagcatc tttccaagaa ttagtggctc atgggcgcga aacagtcttt acttttaaat
    25321 cacgttattt aaaacaaggg aaaaatttag ataatgaaaa agacaagatc gaatacctcg
    25381 aagaattact agttgaactt agccgagttg tttcaccaat cgaacaagat atgtatctat
    25441 ctcaactgtc aacagagttt cagatcggca gagagacgat acaaaaacaa ctgcgcgatc
    25501 ttcgaaggac aaatcagcaa aatcgtcaag aacctcagga aagtcaacac actcaggtta
    25561 cgagacaaga aactgcacgg aaaaagagac ctttgacgca agtagaaaag gcagagcaga
    25621 ttctattata tcgtgcattt aaagaagttt ctgtccgtaa tattttaaaa gaacgagaat
    25681 ttcagtttat tcatgatgca tatgcagaag tttatttttt attcgatgca tatgtctcac
    25741 aacattatga attcaacctt gcagagtttt tagacttttt gcaagatgaa aatttaaggc
    25801 ggttggtggt ggaaatcgaa tatttaccgg tggcagaaga aagttcgcca cgagaaatcc
    25861 aagacttgct agatgtgatc agcaaatctg gcttagctga tgaaataaca ttaaaaaaac
    25921 aaatgcagca agaagccaga cgaactggaa acaaacagct tgagcttgaa ttgacgattg
    25981 aaatcatcaa tttaacgaaa cagttaaaac aagccaaata gctactcaag gggggccttc
    26041 ttttatggca aatggaacag ataaaaaaac gtatgaagca gcagtagcag ctttcatcaa
    26101 agaaaacaaa cccaaacgac aagttgtata tgatgattta tccaatcagc ttgctacacc
    26161 attcactttg aatgcagatg aaatggacaa attgatccaa aagattgaag acgcaggtat
    26221 cagtgtggtg gatgaaaatg gtgatccatc aattcacagc ttgaagagtg ctgagaaaaa
    26281 agctgaacaa gcaaaaacag aagatctttc tgcgccaacc ggtgtaaaaa tcaatgaccc
    26341 ggttcgtatg tatctgaaag aaattggccg tgtctccctt ttaactgcgg aagaagaagt
    26401 agcgttagca ttgaaaatcg aagaaggcga ccaagaagcg aaacagcgtc tggcagaagc
    26461 aaaccttcgt ttagtagtaa gtatcgctaa acgttatgta ggacgaggca tgcagttcct
    26521 tgacttgatc caagaaggaa acatgggact gatgaaggca gttgaaaaat ttgactaccg
    26581 caaaggattt aagttttcaa cctatgctac atggtggatt cgtcaagcca tcactcgagc
    26641 aatcgctgac caagcacgta cgatccgtat tccagttcat atggttgaaa caattaataa
    26701 attgatacgt atccaacgtc aattgctgca agaccttgga agagaaccta cgccagaaga
    26761 aatcggtgcg gaaatggatc ttccaacaga aaaagtccga gaaatcttga aaatcgcgca
    26821 agaacctgtt tcccttgaaa cgccaatcgg tgaagaagat gactctcact taggtgattt
    26881 catagaagac caggaagcaa caagtccagc agaacacgca gcttatgagt tactaaaaga
    26941 acaattggaa gatgtactag atacattgac tgatcgggaa gagaatgtcc ttcgcttacg
    27001 ttttggttta gatgatggcc ggacgagaac attggaagaa gtagggaaag tgtttggtgt
    27061 tactcgggaa cgtattcgtc agatcgaggc taaagcatta agaaaattgc gccatccttc
    27121 tcgttcaaaa caattaaaag atttcttaga ataagcgtgg actattgacc agaagagaaa
    27181 tcagtaaaac tgtttggttt attttaaaag aaaa
//
LOCUS       DADXTH010000035        27073 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.035, whole genome shotgun sequence.
ACCESSION   DADXTH010000035 DADXTH010000000
VERSION     DADXTH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.035"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..74
                     /locus_tag="IWI97_001423"
     tRNA            2..74
                     /locus_tag="IWI97_001423"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IWI97_001424"
     tRNA            78..150
                     /locus_tag="IWI97_001424"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IWI97_001425"
     tRNA            155..225
                     /locus_tag="IWI97_001425"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IWI97_001426"
     tRNA            238..311
                     /locus_tag="IWI97_001426"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IWI97_001427"
     tRNA            323..394
                     /locus_tag="IWI97_001427"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="IWI97_001428"
     CDS             660..1853
                     /locus_tag="IWI97_001428"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBC2462933.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IWI97_001429"
     CDS             1983..3455
                     /locus_tag="IWI97_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2462934.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IWI97_001430"
     CDS             3538..4326
                     /locus_tag="IWI97_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBC2462935.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IWI97_001431"
     CDS             complement(4393..5061)
                     /locus_tag="IWI97_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBC2462936.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IWI97_001432"
     CDS             5176..6888
                     /locus_tag="IWI97_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462937.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="IWI97_001433"
     CDS             6891..8660
                     /locus_tag="IWI97_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462938.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="IWI97_001434"
     CDS             complement(8865..9431)
                     /locus_tag="IWI97_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBC2462939.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="IWI97_001435"
     CDS             complement(9431..10387)
                     /locus_tag="IWI97_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBC2462940.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="IWI97_001436"
     CDS             complement(10387..11043)
                     /locus_tag="IWI97_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBC2462941.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="IWI97_001437"
     CDS             11413..13431
                     /locus_tag="IWI97_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2462942.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /locus_tag="IWI97_001438"
     CDS             13858..16272
                     /locus_tag="IWI97_001438"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBC2462943.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="IWI97_001439"
     CDS             16620..18173
                     /locus_tag="IWI97_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBC2462944.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="IWI97_001440"
     CDS             complement(18554..20338)
                     /locus_tag="IWI97_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462945.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="IWI97_001441"
     CDS             complement(20353..22074)
                     /locus_tag="IWI97_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462946.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="IWI97_001442"
     CDS             22174..22935
                     /locus_tag="IWI97_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2462947.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23902..24170
                     /locus_tag="IWI97_001443"
                     /pseudo
     CDS             23902..24170
                     /locus_tag="IWI97_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24842..>27073
                     /locus_tag="IWI97_001444"
     CDS             24842..>27073
                     /locus_tag="IWI97_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462948.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         8647 a         4764 c         5655 g         8007 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta attccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag tagccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gtgaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 agcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcaa
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tgtaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactgtgac tttcctcagc tatcggtgat
    23401 actaccgata gcattttttt gcttataaaa taattataag tatttagaga tttttcttct
    23461 aacattcttc aaacaagaaa aatcacaaag gttattgtta tttttatata ttctttttaa
    23521 gaatgattgt ttttttatag aaattttaaa aaataaaaca cgttattgat ggatagttag
    23581 tttgttattt ggtaatatta tagaagttga ctggtagtga tttatttttc gtcgttttat
    23641 aaaaatgtta ttttttggaa aaattcacaa atatgtaacg aatgtttttc tggaatttgc
    23701 tgtgttaaaa agcagtatga ctaatataaa tactattgtc atttatgcaa ttttttatgg
    23761 atagtaattt tagaggggaa tcaatgaagg aagaatagaa gggaaaaata gttgcaaata
    23821 cgccagttag caaaaatagc aatacatctt caagctttaa aattcgagag aatcatcttg
    23881 gtagtggaat caactacaac aaaaggatta aaccaattaa tagatagaat taccaatgga
    23941 aaaatagaaa aggtagttat tctatacaaa agttgtttaa ttcgcttcgg ttatgaactt
    24001 agtgaaaccc tttgcaatat atggtacaga atatagaaat tattgacatt acagaaaatg
    24061 atgaagattt aatacagatt gtcactattt gtagatacct tttacaagga aaccgagcca
    24121 ataaagctaa gaagatgatt aaggagttga tggaagacga tacaagttaa gaaagtgaaa
    24181 ctacgcccaa ataaagagca aaaagagtaa ttggacgtgg aaaatgcaag aaatgaacca
    24241 tcgattcggt ggacagttta ttagtaatga cggtttgaga acatccatga cgagaatgaa
    24301 ggaacatcct aaatgcgttt agttgaacca agtgccaaat aatatggcca aataagcgat
    24361 cagttatcga tgaatcccaa gtattctaaa ctatatattc attttgatgc taagcattga
    24421 tatatttttt tgggattgat gaaggttaac caatacaggg actcactgat gaaattctag
    24481 agtctgacct tggtatcaac gatttagtga ttgtttctaa tggaaaagta taaaagtata
    24541 taagtacatt aacaaaataa ctattagggt tacgctccag cttgtgcaag gcactggcag
    24601 gaggaatcgt gaaatcataa ttatggttaa gccacacgga caactaaaag tcatacaaag
    24661 ctatcgaatc aatcaacaga agacaatcgg caatcattag ctttgtttac agcttacatt
    24721 gcaacgcttg agttgaaccg aaaatacgag ggaggtaacg cagccatttt gagttgggga
    24781 atctttttag aaaattatcg ggtaccttgt tgtttagtca aaattataag ggggagtaat
    24841 agtgaggaac aaatttcata aactgttaag tatgtttcta gttatgctta ttgtgatcaa
    24901 tcaatttggg aacataacgg ttctagctga gtctttagca gaacagccag agcgttcgct
    24961 gacgatcgaa gttgatcaag aggaggctgc tttgggtgag cagttccagt tgcgagtcag
    25021 aggaacacca gacctgctaa atgcattggt cattgataag agtgaggatt tgattgaagg
    25081 ggaagtgaag caactcaatg attacgaacg tctgattact tattcttccc agcattcagg
    25141 taaatacgaa ttgaccgcaa gaagcggtga tattcaatca aatacggtct caatagaaat
    25201 cagtgtacca gaagaaagca caagtgagtc ttttaatgta ccagaagaaa gcacaagtga
    25261 atcttttaat ataccagaag aaagcttaag tgaatctttt aatgaagcca gatcatctga
    25321 gccatctata acgcaggaat ctcagattga aagacaagag gagctcttga attatgacat
    25381 cggcatctct gctgagcgcc ccactttgcc gagcaggcat acacaaacgt taaagggata
    25441 cattcaagga ggcgctggcg atattcttga agaatatggg gagatggtcg tacgagttcc
    25501 tagagcatcg attggcttaa ttgagttcat tagagtggag gtcgctgatg attcaccttt
    25561 aaagccactg gcattttacc aagaaggcac agacgttgtt tttccattta cctttgatta
    25621 tgaaggatta aatgaagccg ctcagtcgta tacattcagt atcacgtttg aagttgcttt
    25681 ctttcaatca ggcggtactg agccaaataa tgactttcaa gtgagtctta cttatgctga
    25741 gcgagcagca aacacggaat tttcaattac gcctattcct tcatcaagac ccattttttc
    25801 gaaattatac tatggctcta gagaaatcga cagccaagga aatcttggga atctgctgat
    25861 tgacgggcaa gacgtgacca acagtaataa atttattttg caggtaaatt atgaaggatc
    25921 acgaaaagac ggtgtagtgg ttaccgatgt actaccgcaa ggaacaactt tggcaccgaa
    25981 tccgactaca tggagttcca ctggaaatag agaacctatt tcaggtgggg ttcggatcgc
    26041 agaagtaacg aatccaggtg aagcaaacgt tagaactcat tatgtttccg aggaatttgt
    26101 ggacaaagta tcttttaata aggaaacgaa tacacttgtt gtggactttg gtaatctagc
    26161 agagggtcaa tcgtatttta ttgaatatgc tcttgaagtg accgatgtcg acttgttcaa
    26221 tgaaggaaga caagaggatg ggttttctcg gaatcgagcg agtatgacct acaataatca
    26281 ggtaatgagc cgagactacc gcttgagaat ccctgaaagt aatcttccgg caatctcttt
    26341 aagaaaaagt gtggacaaaa atgttatcaa tctcaatgaa aatgaactaa cgtatactct
    26401 gcatgtctcg atgttttctg gaacaagaaa tacgcttgtt attgaagatg ttttggaaga
    26461 aggcatggaa tttgtgtcat ttctatcaga tattccctct ttcattgatg ccgaagtcaa
    26521 taatggaata gttcgtttca gtgcgaataa tacgattggt ggtccgacaa gttttaccct
    26581 ctcttttaag gtcaatgtag aggcgtatgc ggtcggcgat caaattttaa actttgcaac
    26641 gcttgtgaat agtgacaatc gattgaatac gagtactgta agaacaagga aaatcgatgg
    26701 gcgtattaaa gtaattaaaa ctgatgagaa tggccaaagg ctggcaggag caaaatttga
    26761 aattttatca agcgattcac aagtgataca agaaggtata acggcagaaa acggagaatt
    26821 tttaagtgaa ccgcttcctg taggtagcta tcaagtccga gaaacagaag caccggaagg
    26881 gtatctttta gatgcgacgg tacatgatgt ttttataaat gaatactcca tagaagaagt
    26941 gacgttaacc attgaaaaca tccttgatac gggtggtgtt gaactaatta aacatgctga
    27001 aggcagtggt gaagtccttc aaggagctgt ttttgagtta cagaaccgag aaggagaaac
    27061 gctacaaacc ggt
//
LOCUS       DADXTH010000036        26751 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.036, whole genome shotgun sequence.
ACCESSION   DADXTH010000036 DADXTH010000000
VERSION     DADXTH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.036"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            55..843
                     /locus_tag="IWI97_001445"
     CDS             55..843
                     /locus_tag="IWI97_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBC2462949.1"
                     /translation="MVLLESMPDGYIYSNILLKLYLRSLKHEGKLMFNDRIPFNSTML
                     ATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDIQSFIGKSSTEADRKRKYRKEI
                     EEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEKDIENVTPSKKSKAKPVRHKYG
                     EYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSE
                     SKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            855..1124
                     /locus_tag="IWI97_001446"
     CDS             855..1124
                     /locus_tag="IWI97_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462950.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1129..1293
                     /locus_tag="IWI97_001447"
     CDS             1129..1293
                     /locus_tag="IWI97_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462951.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1286..1588
                     /locus_tag="IWI97_001448"
                     /pseudo
     CDS             1286..1588
                     /locus_tag="IWI97_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1585..1746
                     /locus_tag="IWI97_001449"
     CDS             1585..1746
                     /locus_tag="IWI97_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBC2462952.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1743..2048
                     /locus_tag="IWI97_001450"
     CDS             1743..2048
                     /locus_tag="IWI97_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462953.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2048..2404
                     /locus_tag="IWI97_001451"
     CDS             2048..2404
                     /locus_tag="IWI97_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBC2462954.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2391..2609
                     /locus_tag="IWI97_001452"
     CDS             2391..2609
                     /locus_tag="IWI97_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462955.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2606..3025
                     /locus_tag="IWI97_001453"
     CDS             2606..3025
                     /locus_tag="IWI97_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBC2462956.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3022..3579
                     /locus_tag="IWI97_001454"
     CDS             3022..3579
                     /locus_tag="IWI97_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBC2462957.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3576..3872
                     /locus_tag="IWI97_001455"
     CDS             3576..3872
                     /locus_tag="IWI97_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462958.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3949..4362
                     /locus_tag="IWI97_001456"
     CDS             3949..4362
                     /locus_tag="IWI97_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBC2462959.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            4671..4823
                     /locus_tag="IWI97_001457"
     CDS             4671..4823
                     /locus_tag="IWI97_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462960.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(4820..5095)
                     /locus_tag="IWI97_001458"
     CDS             complement(4820..5095)
                     /locus_tag="IWI97_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462961.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            5549..5755
                     /locus_tag="IWI97_001459"
     CDS             5549..5755
                     /locus_tag="IWI97_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462962.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            5951..6118
                     /locus_tag="IWI97_001460"
     CDS             5951..6118
                     /locus_tag="IWI97_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBC2462963.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            6144..6488
                     /locus_tag="IWI97_001461"
     CDS             6144..6488
                     /locus_tag="IWI97_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBC2462964.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            6493..6774
                     /locus_tag="IWI97_001462"
     CDS             6493..6774
                     /locus_tag="IWI97_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462965.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6877..7191
                     /locus_tag="IWI97_001463"
     CDS             6877..7191
                     /locus_tag="IWI97_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBC2462966.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            7169..8863
                     /locus_tag="IWI97_001464"
     CDS             7169..8863
                     /locus_tag="IWI97_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBC2462967.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8883..10061
                     /locus_tag="IWI97_001465"
     CDS             8883..10061
                     /locus_tag="IWI97_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBC2462968.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            10024..10710
                     /locus_tag="IWI97_001466"
     CDS             10024..10710
                     /locus_tag="IWI97_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBC2462969.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10710..11870
                     /locus_tag="IWI97_001467"
     CDS             10710..11870
                     /locus_tag="IWI97_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBC2462970.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11880..12755
                     /locus_tag="IWI97_001468"
     CDS             11880..12755
                     /locus_tag="IWI97_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462971.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12752..13063
                     /locus_tag="IWI97_001469"
     CDS             12752..13063
                     /locus_tag="IWI97_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462972.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            13053..13406
                     /locus_tag="IWI97_001470"
     CDS             13053..13406
                     /locus_tag="IWI97_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBC2462973.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            13396..13797
                     /locus_tag="IWI97_001471"
     CDS             13396..13797
                     /locus_tag="IWI97_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462974.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13790..14194
                     /locus_tag="IWI97_001472"
     CDS             13790..14194
                     /locus_tag="IWI97_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462975.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            14206..14814
                     /locus_tag="IWI97_001473"
     CDS             14206..14814
                     /locus_tag="IWI97_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2462976.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14834..15196
                     /locus_tag="IWI97_001474"
     CDS             14834..15196
                     /locus_tag="IWI97_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462977.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            15398..18829
                     /locus_tag="IWI97_001475"
     CDS             15398..18829
                     /locus_tag="IWI97_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBC2462978.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            18880..19617
                     /locus_tag="IWI97_001476"
     CDS             18880..19617
                     /locus_tag="IWI97_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2462979.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19627..21918
                     /locus_tag="IWI97_001477"
     CDS             19627..21918
                     /locus_tag="IWI97_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2462980.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21942..24068
                     /locus_tag="IWI97_001478"
     CDS             21942..24068
                     /locus_tag="IWI97_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462981.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            24085..24234
                     /locus_tag="IWI97_001479"
     CDS             24085..24234
                     /locus_tag="IWI97_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462982.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            24231..24677
                     /locus_tag="IWI97_001480"
     CDS             24231..24677
                     /locus_tag="IWI97_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462983.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            24679..24816
                     /locus_tag="IWI97_001481"
     CDS             24679..24816
                     /locus_tag="IWI97_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBC2462984.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            24854..25147
                     /locus_tag="IWI97_001482"
     CDS             24854..25147
                     /locus_tag="IWI97_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2462985.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            25144..25368
                     /locus_tag="IWI97_001483"
     CDS             25144..25368
                     /locus_tag="IWI97_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBC2462986.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            25365..26390
                     /locus_tag="IWI97_001484"
     CDS             25365..26390
                     /locus_tag="IWI97_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBC2462987.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            26580..26653
                     /locus_tag="IWI97_001485"
     tRNA            26580..26653
                     /locus_tag="IWI97_001485"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26614..26616,aa:Ile,seq:cat)
BASE COUNT         9479 a         4063 c         5680 g         7529 t
ORIGIN      
        1 aacaaacgat actactattt aaaactaaaa gagaactttt tcgatagcga cgagatggtt
       61 cttttagaaa gtatgccaga tggatacatt tattctaata ttcttctcaa actttattta
      121 agaagtctga aacatgaagg caaactaatg tttaatgaca ggattccatt caattctaca
      181 atgcttgcga ctattacaag acactctgtg ggagttgtag aaaaagcagt acaaatattt
      241 cgtgatttac aacttattga ggtattagat aacggagcaa tttatatgtc tgatatacaa
      301 agttttattg ggaaatcttc aactgaagct gataggaaga gaaaatacag aaaagaaatt
      361 gaggaagcaa aacagaattt aataacaagt ggacaaatgt cggacaaatg tccggacaaa
      421 actacaccag agttagagat agagttagag aaagatatag agttagagaa agatatagag
      481 aatgtaacgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata cggagagtat
      541 aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaatagaatt ccctaatgac
      601 taccaagagc gaatagaacg gctatctgag tattgtgaat catctggtaa gacttataaa
      661 aactatttgg caactattcg aagctgggca agaaaagaaa aaagtgaatc taagaacgca
      721 agcagtggat acaagcgcac aggaagacga gagaagcttc ctgagtgggc aatcgaccaa
      781 gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc
      841 taagaggtgg gaaattgaaa atcgattatc tagaactaat taatgaaata gcaaagtata
      901 aaactggtga ggaaatagaa atcctgagag acgtatatga tcaactcgaa gaagctggaa
      961 tcgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat ttcgcacttt
     1021 atatcgatac aacacaatta agaaatttag catataccaa attactattt gttgattgtg
     1081 ttaagggctt gcaaaaacat cttaatgaac ttgagcaggt gtaatcagat ggacctaaag
     1141 acatttacag cacagattga actaatgcat caagaagctt taagacaaag tgtatcgtac
     1201 gaagacaagt ggctcaacac gttccacggc ggacgtgaga gcgcacttga tcaagtactc
     1261 aaattactga aaggagaatg tcgggatgga taagaaagcg gcaatgcagt gaattatcga
     1321 attgacttat tcagaagatt ggcaaaatga caaagaagct gcttcagaag tgatgaggct
     1381 tggaagagcg atgtgggcag acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg
     1441 gcacggtgac aaacttctag tgatagggac agctgaacag ttagcaagtc tcacaggctt
     1501 gcacgagaaa atcgtgagga aaagagcaag gtgtggctac acagacgcta agaagagaac
     1561 gtttagatac gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
     1621 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
     1681 gcgagaaagg caatcgattt gcacatagag aatttaagaa gggaaatcca tcgattggag
     1741 gaatgagcgt ggataagaaa gcagcaatga aaagaattgc tgaattaacc aagtcagaat
     1801 cttggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
     1861 ctgaaaagcc caaacggaga acgccgagaa aaattgcaat ctggcatgat gaccgaattt
     1921 tagtaacagg tactgctgaa cagttatctg aaattactgg attaagcaaa aacattatct
     1981 gggatagagc taggagctta tggattgatt caaaaggacg acagtttagg tatgtggagg
     2041 agagataatg gatctcatta cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa
     2101 gattcagaaa gataaaaaat caaaagaacg agctgaatta gttaagttag aaatggctga
     2161 aacaggagca ggagtgcgaa gtagcaggca ttggaaagca gcagcaaaca ttgaatttta
     2221 ttacaacgaa attcaaaaag ggttcgatca gatgcgtgag ctggatcggc aaacaaattg
     2281 gagcaagaaa cttcatcaag atcgtttcaa atttgtagag aagtataaag aaatattaga
     2341 agagtatttg aggaggacag caaatgataa aaaaactcgt tcaattcagc atggatttat
     2401 atgatatcga atcaggagca acactatctg tggaatcgga ccatctaatc ataaattttg
     2461 gtggaaagcg ccagattatt ttgtgggtag ttgatgatgt actgtttcca gaaattgttc
     2521 atgatttcga agaatcaaaa gcggttgagt ttgaaatagt gaaaaaagta atggaattga
     2581 ttgaaaaata cgaggaggac agcgaatgat accgaagttt agagcgtggg ataaacgaaa
     2641 gaacgtaatg agagatgtag ccgtcttgca ttttactaaa aacggcaaaa caaactttat
     2701 tgaatattgg ataaatccta ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat
     2761 gcaatccaca ggaatgaaag ataagaatgg tgtggatata tttgaagggg atatagtatt
     2821 agtcagcgtg cgaaatggct tcgattactt agataataaa gtctgtattg tcaaaaattc
     2881 aatagattat tccggattag tttgtgccac tgttgatgaa gacttagagt atcaaatttt
     2941 taacacagag ctgtttgaag aatacacgta cgaagtcacc ggaaatatat acgagaatag
     3001 cgagttattg gaggaacagc gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt
     3061 aaagcgatag gaaatgatgc aatagctgct tgctataacg agagtataaa ttcaggtatt
     3121 acgttaatga aaaaaataga cgaaccgcag aaaccagtta ttccacaact cgtggccggt
     3181 tggcttgaga aatctacgga cccttttaca aaagctgaaa aaatagcgta tttaatcaaa
     3241 tctaaagatg gtgattcata ttatttctgt gattggtttg tacgagatgg catagtgacg
     3301 caagagcaag gagaagaatt actcgcttgg gcaacaagac aatcatatga aacactattg
     3361 agcctataca acggctacga ggttgagaaa gagccgttat gggcaataaa gaatgccgat
     3421 ggaaactatc ttactaaatg tgctttatgg ggaaaagatg gagtaaatta tagttttgaa
     3481 tgcaatccat ctcatcgatt gcttttcact gataaagcaa cagcggatgc tgcagcattg
     3541 ttggtgaatg gaacagtgga agaggtggta gaaagatgaa actaaaagac ggattttacg
     3601 ctagcagtca cggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac
     3661 gcaagaaatc aaaattcaaa gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt
     3721 atccgttcag aggatacgtt aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa
     3781 atacaatgaa gtgtgatcgt tcgactgcaa aacacaagca ttatttagct gtagcgagat
     3841 tagttgacat ggaagtaatc aagagcaaat aaaaaaagcc ggatcgctcc gactgattca
     3901 ataaatccaa cacatttatt atatcacata aaggagcggt ttgacttgat gcaattgtta
     3961 cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
     4021 cgtttggagc ggatggcagg tcgctctttg atagatatta agtcgccgat tataacggat
     4081 atgccgaagg caccgaagca tggcaataag gcagaagacg cgattattca gatgatggat
     4141 atagaaacgg agagagatgc gattctagcg gctttgatgg ctcttagtct gattagtcgt
     4201 cagatactct actacagctt ctgtgtgcca gatagcttct caaactacag aattagccgt
     4261 gaagtgggtt attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt
     4321 gcagaagcat ataaacacgg aaaaataatt gcttataaat aatttggcgg ttttttggcg
     4381 gaatgatggc ggtttttaga catttaccag tgttattatg gtagtgtcga aagattagga
     4441 aacaggatcg acaaaataaa cgaagggagg caatctccct catcgtttta aacttcttct
     4501 tagatagaca gcaactaatt atgaagaaaa ggatgtgaat tcaactcctc ctaaattctt
     4561 catcggttgc tgtttatttt gattagacag cagcactcac aaaactaaac atgttcttgt
     4621 cgtctttcaa ctgctgctgt ctattaattt atgtattgga gggaagaaaa atgaaattat
     4681 cagtagaagg cactccagaa gaaatacaag aattgctcca agctattggt agtagcaagg
     4741 agcaaaaggt aagtgtttct aattcagata ttgacggtat ttgcaattgt ttaaacagag
     4801 aaaaaatatc taatccttac taagagaaaa tatgttcgtt aatgaatttc caattttcct
     4861 ctgtagctct accttgataa tttttttgca gttcacagat gaataaggaa tcgttattat
     4921 caagtactgt atttaatttt tcacacattt gatttggatt caaattagtt ttaactaacc
     4981 aaaaagattt ttgtagcttt atatatgccc caaattcttt tattatttca aatactttat
     5041 catacttttg ccctgggtta ttaagatcat aactaatcat gtagggttta tacatgttgt
     5101 cttgcacatc cttgttttat atttcagcgg accactcgct gataaataaa attatacgct
     5161 tagtatttat tttcacaata ttaatttatc gtaataactt ttgagtaaat agaacaaaaa
     5221 acctgcacta gtttccgcta gtacaggcag tgactatatc ggttgataat ctagcatata
     5281 ttaaaaataa ttgcaagaaa agaaatttat agtggtttat accaaattat caaagcaatg
     5341 ttattttgtt gctgtctatt gttttttaat tattcacacg ataactaaag gtgggtgaag
     5401 agaaatgatt ccattaataa tttcaatttt tgcgctctgt cttaatgtct atatgattgg
     5461 atttaaaaat gggcaaaata aaaaatagta gcagccaaga ataattttat agtgtcactg
     5521 tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca accatgcaag
     5581 gttcgattcc ttgccagcga cattaaatgc ctatgacggt tacgactacc gaaaaaagat
     5641 cgttaagaag ctatacggtg ctacgtacgg caatgtagta agtgtgctat ctgtacacca
     5701 ccaagcttcg gtcactgtgg cggaagtaga agacgcagcg gtaaatggcg agtagcctcg
     5761 tgagagcctg gtaagttctc gtgagtggtg caatcccact ccagcgactt taagcaaccg
     5821 aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg
     5881 caagttacta ttacatatta gatcactctt tgagtggtct ttttattttt gcacaaagga
     5941 ggaaacaaca atgtatagac cacaatactt agaacagaag tatgaagtaa tcactgttca
     6001 taaaggtaat ggcgaaaaag tatatgagta tagaagacca ataaagagcg atacatataa
     6061 acgaaaggaa aacaatgaag ttattccatt gtatggcaaa agaatagcta agcattaaat
     6121 aagattgcga aaggagacgg aacatgaccg aggaattcta tagatggcta ttacagttga
     6181 caagagaaga tcgtttggtt aagttctatc agtctcctaa atggcgcagg cttagagaga
     6241 aagcgatgaa acgagatcac tatgaatgcc aagagtgtag aagactaggt aagtatcata
     6301 gagtagagaa cgttcatcat ataaaggaag tcaaggatag acctgactta gctttagatt
     6361 tagataatct tatttgttta tgtgttgaac atcataatga agttcatggc agatatctta
     6421 cagcattaga taaacaagag aagaagatag aaagctttgc taacttcgat gcaagtgaaa
     6481 ggtggtaagt gcatgatcat caatgataat ggcagagagt atgatacaga aaagattgaa
     6541 gagtattcat cttatactca gggattaatt aaacgtttga tatacgttcg ctatgtaggt
     6601 attagggatc tgttatcaga taactgttgt agtaaataca aagtgaatca agtaagagaa
     6661 gcgttgaata aagataataa cgtcgaaaga ataaaaaatg tttttggata tagtattgaa
     6721 gagattaatt attacattga cttcgctgaa gctttcattc cgatggtgag ataacccccc
     6781 cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt caggaaaaga
     6841 gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa
     6901 aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag tcagaaaaca
     6961 aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga aacggcgata
     7021 tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa aaagaatgaa
     7081 tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga taaacttgcc
     7141 attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata agcatattga
     7201 aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc aattattgct
     7261 aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg ataagcagat
     7321 ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa ttccgtggga
     7381 aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa cactagtgtt
     7441 caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg cttcttggaa
     7501 caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg atatctatgc
     7561 caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa ttaaagatca
     7621 tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa atatcgctac
     7681 aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga agcgaccagg
     7741 tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa atgtggctac
     7801 ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta atggtcatgt
     7861 tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt ctggagaact
     7921 tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt gtcgcttgga
     7981 taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa ctattaatta
     8041 caatgcagat ttaaaacgga aaatgtttca agaatactct caaatgcaac gtaatgctgg
     8101 tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata cacgatttgc
     8161 tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg aaaaaatgga
     8221 tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg ttgggttgct
     8281 aggaaaatac gataaagatg tttattttac acaacatact tttatccacg aatcagccct
     8341 tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag gaaaatcaca
     8401 gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc ttgaaatgag
     8461 taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc
     8521 cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg ttacacatgg
     8581 tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt ttggtgacga
     8641 tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta atggaaatat
     8701 acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt cattccttca
     8761 tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca caaatagctc
     8821 agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa ggaggtgaga
     8881 aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg gttcctttag
     8941 aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg attgaaactt
     9001 gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat gaagatggaa
     9061 aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag aaaaataatg
     9121 caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa gtattgatcg
     9181 tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat gtcacagaat
     9241 atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc cttgatagag
     9301 aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt aggcaactag
     9361 tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat gtttacaagc
     9421 gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa gacaatacaa
     9481 cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg gaagctgata
     9541 atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc agcggaaact
     9601 tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag atgacagcag
     9661 cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta tcggatcaag
     9721 tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag gatgcgttta
     9781 acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt gtggatacaa
     9841 ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta ggcattagaa
     9901 atggagtatt tacaatcaac gaaggaagag aacgcgttgg aaatgatcgc tctgataagg
     9961 cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg
    10021 cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt accgcaaatt
    10081 tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga caagggaatc
    10141 aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat taattcaaac
    10201 ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa taaagcaaaa
    10261 gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc aatggctggc
    10321 gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc cacatacgga
    10381 gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat tgataaatcg
    10441 gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca agaacttctt
    10501 gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt cgatgaaatt
    10561 atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt
    10621 ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc agcattaaat
    10681 aaaacatttg gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag
    10741 ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct tttagcgcag
    10801 ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca acagcaaaaa
    10861 atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca caagaaacaa
    10921 aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa ttagtgccag
    10981 ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa
    11041 ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga gtcaatccag
    11101 catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatggc tttgatgtaa
    11161 ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca atgcttgatt
    11221 taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca ttgagaattg
    11281 cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga atgatgcgtg
    11341 acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct attacagctt
    11401 tagatgctgt aactatgggc ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg
    11461 tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa
    11521 ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat ggagaatacg
    11581 tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag
    11641 cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta aaaattgaag
    11701 cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa
    11761 acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta
    11821 ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag gtgatcagaa
    11881 tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt
    11941 taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg
    12001 atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg tacgatgatc
    12061 tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg aaatatttag
    12121 aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat gtcattttca
    12181 ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat tgtagcgact
    12241 gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa aatattctaa
    12301 aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata aacactaatt
    12361 caggaggcgc acagtgggtt attaaccgtc ctaaagcgaa ttattggaaa aaggtttatc
    12421 tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata caaatctgga
    12481 cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt cctaaagttc
    12541 acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa gaagtaaaaa
    12601 tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa gggagatacg
    12661 tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa tgctcttacg
    12721 catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga ctgaagaatt
    12781 gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg agtcaattaa
    12841 aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt
    12901 ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg actggaatcg
    12961 tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca ttcaacatta
    13021 tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa
    13081 cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat gaactaggaa
    13141 aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat ctttccatga
    13201 gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag aaagtaaaga
    13261 ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata
    13321 gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg
    13381 aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa
    13441 atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc
    13501 gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata
    13561 gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca
    13621 gaaaatagag aagatttaac aggagactca ctagatatta tttcattgat tcaaaatcgt
    13681 tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat
    13741 atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa
    13801 tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc
    13861 aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt
    13921 ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca
    13981 cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc
    14041 tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa
    14101 tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact
    14161 tctgattcgt caattcgata aattaaataa atagggggaa caacaatggc taaaacaaaa
    14221 actgtagtaa caacgttcga taacgtaagt atcaaacgaa ttgcttttaa ttttaagaac
    14281 gcaggaaatg caatcgcaac gaattgtaat ggacaattag atggcgaaac agaaatgcaa
    14341 acggtggtta aaaaatgtgg agcgacagaa gtaaaatcaa aatctaaacc aatcaatatg
    14401 acggtaacaa ttactgcaca tgtaccgatg gaagtttatc gacgtttcaa tgggttgaaa
    14461 caagatggac gtattaaacc aggcatttac tcttacggtc ctgattccgt aggcgaagat
    14521 ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa atagaaagtt agttggtatg
    14581 ttagcatgca cttcgaatac aggattaaca ttctctattg aaaatggtgc ggatgaagta
    14641 gctgcgttag aactagaaac aaaagttatg caagatgaat ttggtaaatt ttatcatgaa
    14701 gcaattgttg cagaacttga agaagactta acagatcaat ggatgacgaa tctatctgct
    14761 gatgtgatta aaaaaacttc agttgtgaca actacggcca ctcaatcaca gtaaaaaaaa
    14821 cggaggtagc gaaatgaacg aagattactc aaaaattgaa ctaaacgatg gaacaatttt
    14881 gaatttagaa cctaaactga atatcaagaa attattgatg atcaatagag attttaacac
    14941 agacgagttt gcaaaaatga ctgtgggaaa aggatccatg gatatttctg ttattcaagg
    15001 tgcaaaggct gtgtatattg cttaccgcca agcgaacatg actgattata tttcattcga
    15061 tgaatttatc gataaatggg attttgatat ggctactgcc agctatattt atcaattgat
    15121 gatgttcaaa caagcacgcg atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa
    15181 aaagcttcaa aagtaaaaat tccaaagctc ttagttgaaa cgtgggtcga tgtctattcg
    15241 atgttgaccg acgttttttc tatgccttca gatttggttt taagcgatat ctgtttagat
    15301 gacattttgc aaatggctta caacaagagt gcttatgaag gatggaaaaa ctatgcaata
    15361 aaccaatccc agaaaaacta aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc
    15421 agaagtaact ttcatagcta acgatgacgg attgaaatct acgttaaaag aaatcagtgc
    15481 tgaattaact aaaaatagag cagaattaaa actagaacaa gctcaattac aacagactgg
    15541 ttctgaatca gacaagttag gaagtaaatt atcttcttta gaaaagcagt atgaattaca
    15601 aagtcaaaaa gttgaagtaa ctagccaacg tttagccaat gcgaaaaaat attatggaga
    15661 aaattccacc gaagttcaga aacttgaaag agaactgatt aatcaacaaa cagcgcaaca
    15721 acgtttgtca aacgaaattg ataaaacgag taatgcacta gctcaagcaa aaggcgaaat
    15781 acagacgtac gagtctacaa tgcaacagtt ggatagcgaa caaaaaaatg ttcaagctag
    15841 tgcttctctg attgaatccg aatacaaaaa atggcaagca actgctggtc aatcagcttc
    15901 tgaagccgag aaattagcga aagcccaaga atatgtttct caacaatctg aaaatgcgga
    15961 gaaaacgata gatatcctaa gacgacagtt agaagctaca cagtctgaat ttggcgctac
    16021 atccacagaa gcaatgcaga tggaagcgaa gcttaatgat gctgaacgtg aatttgaaga
    16081 gttaggacaa gctgctaaaa atgtagatac aactaacttg gacgatatcg gaagcaaaat
    16141 agatatgaat aatttaatgg aagcttctga cgttttaagc gacattggcg ataagcttac
    16201 agaattaggg aaacaagcag tggactctgc taacagtgta ggtagttccc agagtaagat
    16261 acaagctaat tttggtttgt ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga
    16321 catttattat aaaggttttg gagaatcgtt agatcagtcc acagatgcat tgattttggt
    16381 aaagcgtaat ttaggagatt taaataatca agatttacaa aatatcacgg aacaagctat
    16441 ggtcctagaa aacaccatgg gcgctgatat ggatgaaacg ttacgtggtg taaatggctt
    16501 aatggtcaat ttcggcttga gtgctcaaga tgcaatggat ttaatggttt cgggtactca
    16561 aaacggttta gataaaacgc acgaattagg cgacaatatg gcagaatata gccaattatg
    16621 gagtcaaatg ggatattcag ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc
    16681 gggtgcttat aaccttgata aagtcaatga cttagttaag gaaatgggaa tatcgttaac
    16741 agatggtcga tttgagcaaa acatggatat gtttagtgaa agtactagaa aagcttttga
    16801 agagtggaaa aatggcggag gaacacaaaa agacgttatt aattccatga ttcaagattt
    16861 tagcaatatg gatggtcaat acgaccaatt aaataaagct tcgacaattt ggtctgcgct
    16921 tggcgaagat aatgcgatga aagttgtcca atctttaact gatgttaacc atacatttga
    16981 tgatgttagt ggatctgcac aaaaaatgaa tgaagattct actactccgt tgcaagagtt
    17041 gaacgggaaa atagctgaat taaaggattc attagctcct ataggcaaca caatcataga
    17101 tgcactcgaa ccagtaattg attttctagg aaagatggct gatgcgttta ataatcttcc
    17161 acaaccagta caggatttca ttgttgctat aggtggtctt acagcagcat ttggcatact
    17221 gttaccggca atactagccg tgtcatattt atttggtccg atgatgctta taattggagg
    17281 aattatagct gttatagcag gcgttattgt ggcaattaag aactggggtg caattactga
    17341 ctggtttagt ggtttatgga aaaaatttac tgattggttg ggtgatactt gggaaagtat
    17401 aaaagacgga gcctcatcag tttgggatgg ggttaaagaa acctggtctg gatttgtaga
    17461 ttgggttcaa gatatttggc aaggagtttc tgattggttt ggagagctat ggagcggatt
    17521 agttgaagga gcttccaaca tctggcaagg agtccaagaa acttggcaaa cattcgttga
    17581 ttgggtttca aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat
    17641 tgtaggaatt gttcaaatac catggacatt aataacgtca ttgattcaag ctggtattaa
    17701 tattatcgtg ggtatttttg atgtagctgg acagttattg ggcgcagctt ggcaagctgt
    17761 ttggacacct atttctgatt tccttaaaaa tacttgggat actatgacac aatggataag
    17821 tatcgcttgg aatgggattg taactacatt ccatactata tttgatccag tagtggcatg
    17881 gtggaatggt atatggacag ctattagtac tacggcttca aatatttgga attcaattag
    17941 tgcaacagct tctagtattt ggaacagtat caagaataca atcactagct tggtacaagc
    18001 agctgctaca gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg
    18061 gaattcaatc agctctacag catcaaatat ctggaatagt gtgactagta gtataagcaa
    18121 tgctataaac gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat
    18181 tagcggaatt tggaacggta tcaaaaatac tgctttgaat ctttggaatg gaattacaag
    18241 cactattagc tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct
    18301 aacaggtatt gtttccgaca tattcaataa tgttaaaagt acaattgcta acatatggga
    18361 aggcatcaaa aagactgtta gcgctccaat tgattggatt agagacaaaa tcagtggcat
    18421 ttttgataat ttgaatattt ctataccaca tattccgtta ccggaattta tcatggaagg
    18481 cagctttaac ccgctaaaag gtcaaatccc ccatttgcgt gtaaaatggc atgctaaagg
    18541 aggtatcttt actaaaccaa ctttactagg tggaatgaac ggtgtcggtg aagcaggacc
    18601 tgaagcagtt ttacctttga aaagatctgt tttgcaagaa attggtgatc gtatcttgag
    18661 tagcacatca gtttcatcta gggcacaaac gattcaacct gtgaataact acgaattcaa
    18721 tttcacaatt gatggtaacg cagatgaggt tactatgaag caaacaactc aacaaatcat
    18781 tgatagcatt acaaaaattc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18841 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18901 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18961 ccttctccaa aaagacagag agttgaaact tctgttttag gtaggttagg tggcttagta
    19021 caagattact cgtttgaaga tatgtcgttt acattgcact ataactattt agaggatgtg
    19081 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    19141 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    19201 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    19261 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    19321 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    19381 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    19441 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    19501 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19561 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19621 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19681 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19741 agcaataaac taaacggagc cgagatattt cagggtactt attcaataaa cggaactaat
    19801 gcagatttga ttgtagacgg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19861 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19921 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19981 gaaaagatca ctgctactca ggcatggcaa aacgcaaaag ttttagcgaa accagctatc
    20041 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaag
    20101 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggt
    20161 gagtttctaa aagataataa cacattacgt catgaaaaaa gtctaggtac ggatcataaa
    20221 atcaaagcga tttatactaa aaacttaact ggtcttgact tagagattga tgctcaaagt
    20281 gttttagttg gagtttatcc attcattagc agctcttcag aaggagaaga cgagatcact
    20341 ctaccagaag aagttatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    20401 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    20461 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    20521 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20581 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20641 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20701 aacttcttag aaaacacaga gaataatgtc agcaatttga ttaatgatgc cattgatcaa
    20761 ttgaaaaacg gtggcgaaat cagtgattta ctcaatgata ttgtagatca tcaaactgat
    20821 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20881 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20941 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaacggc atggacgtta
    21001 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    21061 ggtactacat taattagtga tggtgctgat tatagaacaa gtattgctaa tggcaaaatg
    21121 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    21181 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    21241 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    21301 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    21361 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    21421 actatgaaat ttactagtga tggtatgcta acgttaccag gttgtttttt tggttcatgg
    21481 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21541 gttagaggta ctaaaaactc aactgtacca acagaacatt atggacaacg actattgaac
    21601 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgacaat
    21661 ggtaaagttc gtgttgatat tgaccccatg tttgctgaaa cagtaaatct aagtcggtat
    21721 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21781 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21841 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21901 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21961 atttggcttt agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    22021 gtgataaagg gattacggtg acggttaaac cgtttctaaa tggcttgcag atggatacga
    22081 gtggcggaac atttacttta aaaggaacaa caccatctaa ccgttacgta gataatgttg
    22141 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    22201 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    22261 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    22321 atgtttcgca attagaagag ttgattcgaa agtacaacga aacttttgat gcttttatgg
    22381 ctgaaatcaa aggtagagtg gatagcttaa atcaacagat tactgattta actggtcaag
    22441 ctaaaacgct acaagacaag ttagatgctc tgaaagaaga aatttctaag ttaggtaact
    22501 tacaagtgat gtacagtaac agcatcgact tcggggacta tgattattcg gagaacccga
    22561 atttaatgcc atatattaca gaaccttggg ttggaccttt gttaggtaat ggacatacag
    22621 ttaaagatag tgttaaaagg gttataactc atactaaaac tagaacagct aattccggcg
    22681 acatattatc gcttggctta ggtattccgt gtacagcgga agctaataat aggtatctta
    22741 taaccacttt acgaccatct acgacataca cattaagcgt tactatgagt gtcggttcag
    22801 attggactgg agagaccaat actatagggg ttaggcttag atatttaaat gaacaaggtg
    22861 gtatagaact gcccattaat gcattaatac cagcaaatgt agaacgcgat aaaatggtca
    22921 ctcatacatt tactggtata acaaaagata atgtaactag cataacaaac tgttatgttg
    22981 aaatattctc acttaatagt gaatataaag gaacggttag tgtcagctat gacgttaagt
    23041 taaaagcaca ctaccctaac ttactagatg gcccttactg gttaggtaaa gttcctttag
    23101 gcgagaatat tgctgaccct acagttgtct ttccacacaa aactagtgaa tatatggttt
    23161 atggtagacg caatacagaa aactatatcg ctgaccaaac atataccata tcaatgaaag
    23221 caactaaact aacagttcaa tcgtttgccg tgtatattgc tgctggccgc gttaaagtag
    23281 gagacatgaa gcctactgag gggttagcta atacatggga gttaactttt actgtaacta
    23341 agcaacatat agatagtggg gtaactaatt atctggaaat ataccaatac ccgtcagcta
    23401 caaaaggagc tgttcaaatt gagtggctca aactagaaaa aggcaatact cgaaccccga
    23461 atattagtga gtataaatat cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt
    23521 atgtttggga tctagcacca gaatatgtcg aagataattt ggccacagat attaaaattt
    23581 ctgaaattac tggtaaagca aacaattata ccgatgggaa agtatcggag attaattcgc
    23641 agttgactgc ttcaattaat gaagtagaca ccacagctaa ggatgctcaa acaaaagcga
    23701 atgctaatgc gactgctata gatgaattag acaataagat cgatgaacgc attaatgata
    23761 cagctactac cacattaaca gttacaaacg ggaataccgg atcagcaaag ctttatcgtg
    23821 aaggaaaaac agtttctata tattttgtgg ctttaaacgg aaaaagcagt ggtggaaatg
    23881 attcaacgat actaacaatt ccagaaggct atcggccacc aattagtttt gagcaactgg
    23941 ttggctcgat agaccgttct actttgaaca gtgctcagtt atctattggt gcagatggag
    24001 ccattaaatg gcgaagaaac tcaagttatg gatcggatta tacctttgca attacttaca
    24061 cgatttagaa aagcgtgaat cgatatgaag gcagcatata gaccaattga accttacgga
    24121 ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg aagaatgcac agaagtcgaa
    24181 ccaccgattc caaattggaa accgaaattc aattactggg agggaaataa atgaaaaaca
    24241 tttggaaata tggacgtact ggcggagagt acgcaggaaa agtattggac gacatgcttg
    24301 tatccgttcc ttacacagat cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt
    24361 taacaatcgc tgatcaaatg tttgatccta aattgaatca atggattatt ttagcgaacg
    24421 cactagatca caacgattta aacaatctca aagcaatgta tgagtcgtta gaaaatgaga
    24481 acggcgattt aaaacagatc aatgccaaac tcatgctaag cgatgtagca attaaacagg
    24541 aaaatactgc attgaaagaa aaagcggata gtttagcaca aatcaattca aaaatgatgc
    24601 ttgcttcgtt acaaaatagc aaagacattt cagaaattaa agagcaacta aatccagctt
    24661 caaagggagg tgagtagtat gtttagtttt agcgatgtga aaatgatgta tgattggggc
    24721 tgtttcacta acgaacaagt aatggttttc gttccgttgt gcattactga agaaaaagca
    24781 gataaaatca ttagcaaaga agagagcgca tcttaattga tgtgcttttt attttgattc
    24841 aaggagttgt cacatgatta atttagggga atggggagcg atagcaggat caataaccgc
    24901 tatcgtttct ttgattttat tagtaataaa accaattact gcatctttct cgaagattac
    24961 tgagactctt tcaaaagtaa gtcacaattt agatttgctg actaaagatt tagaatcgag
    25021 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
    25081 acatgcagaa aaattggtag aacacacgca acaaattaaa actttattta gggagagaag
    25141 aaaatgaata ataaaacgtt cgaagtacta aaatggttcg cactggtaat tattcccgca
    25201 ctagctactt tcgtggggtt agttggtaaa gcgctcaatt ggcagtacac agatatctgt
    25261 gttgtcatca ttactggttt tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga
    25321 acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta
    25381 ctattactgc gatgagcctg ttaacggctc tttttttatt acccattaat acgtttgctt
    25441 atactattaa tgacgagtat aatttagcgc cgaatcaagg agactccaga ttagcaattc
    25501 ctaacaaaat tattttgcac gaaactggaa tagatgcacc agcaagaaac gtagccgcca
    25561 acatgaaaaa taattataac ggaagcaatt cttatactac agatgttatt ggtgacggtg
    25621 ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg gggagctggt aacgctaatc
    25681 cttatgcgcc tgtacagatt gaattacagc gcacatatga taaagcattg tttgaaaaaa
    25741 actatcgagc ttatattgaa tatacaagag atagtgcaaa aaaatatgga attccattga
    25801 ctcttgatca aggaacttct ttatttacaa aaggaatcat ttctcatttg tgggtgacaa
    25861 attatgtttg ggggaaccac acagatccat atggttactt atcgcaaatg ggagttagca
    25921 aagaaaagct tgcttatgat ttagctcatg gatttaccga tgaaaatcca acaacttctg
    25981 aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc taatcctacg ctgacagatg
    26041 gaacaaatca cgcccacatt gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg
    26101 ctggatggca cattgctaac tataaatacg agtatatttt cattatggac tacaatactg
    26161 ggaaagaatt agctcgagta agagctgatg gaatttatag accagatgta aaccaagctt
    26221 ataatacttc tggaaatgtt ggttatcatg tatctttcaa tatgcgtaat tttcctagta
    26281 agaaagtcta tgtaatgatg cgggcaacga atgatccaga gggaaacact aaaggcggtg
    26341 cgcaagattt ccatgacaaa cgttggtatt taaatattcc taaacgataa aaatagctcc
    26401 tcgttgagga gcagtacata actatattga caactataaa aattattcga taaaatagtg
    26461 atgttatcgc atatcttcac tatcacccat aaatagtcac actccaagct atgcgataac
    26521 aggtttgttg ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag
    26581 tacccttagc tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt
    26641 cctacagggt acattaacgt agccatttga atcgttctgt gttagaattt ttttgaagag
    26701 tattatacaa gctaaagctt ttcttcattg ccactcaaat gagtggcttt t
//
LOCUS       DADXTH010000037        26151 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.037, whole genome shotgun sequence.
ACCESSION   DADXTH010000037 DADXTH010000000
VERSION     DADXTH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.037"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            227..1231
                     /locus_tag="IWI97_001486"
     CDS             227..1231
                     /locus_tag="IWI97_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462988.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1232..2176
                     /locus_tag="IWI97_001487"
     CDS             1232..2176
                     /locus_tag="IWI97_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2462989.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2176..3138
                     /locus_tag="IWI97_001488"
     CDS             2176..3138
                     /locus_tag="IWI97_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462990.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3165..4079
                     /locus_tag="IWI97_001489"
     CDS             3165..4079
                     /locus_tag="IWI97_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2462991.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4105..5886
                     /locus_tag="IWI97_001490"
     CDS             4105..5886
                     /locus_tag="IWI97_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2462992.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNDAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6234..6920
                     /locus_tag="IWI97_001491"
     CDS             6234..6920
                     /locus_tag="IWI97_001491"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBC2462993.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6940..10521
                     /gene="smc"
                     /locus_tag="IWI97_001492"
     CDS             6940..10521
                     /gene="smc"
                     /locus_tag="IWI97_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBC2462994.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEVLAKEQPKMYQLMNQVQQLRARQKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCSVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10530..11339
                     /locus_tag="IWI97_001493"
     CDS             10530..11339
                     /locus_tag="IWI97_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2462995.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11351..12349
                     /gene="ftsY"
                     /locus_tag="IWI97_001494"
     CDS             11351..12349
                     /gene="ftsY"
                     /locus_tag="IWI97_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBC2462996.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12536..14803
                     /gene="gshAB"
                     /locus_tag="IWI97_001495"
     CDS             12536..14803
                     /gene="gshAB"
                     /locus_tag="IWI97_001495"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBC2462997.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTGRNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFADQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14892..15344)
                     /locus_tag="IWI97_001496"
     CDS             complement(14892..15344)
                     /locus_tag="IWI97_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBC2462998.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15494..16444
                     /locus_tag="IWI97_001497"
     CDS             15494..16444
                     /locus_tag="IWI97_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBC2462999.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLAT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16437..17396
                     /locus_tag="IWI97_001498"
     CDS             16437..17396
                     /locus_tag="IWI97_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBC2463000.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     GFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17393..18148
                     /locus_tag="IWI97_001499"
     CDS             17393..18148
                     /locus_tag="IWI97_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2463001.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18171..19124
                     /locus_tag="IWI97_001500"
     CDS             18171..19124
                     /locus_tag="IWI97_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2463002.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPNLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19647..19943)
                     /locus_tag="IWI97_001501"
     CDS             complement(19647..19943)
                     /locus_tag="IWI97_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBC2463003.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20298..20654
                     /locus_tag="IWI97_001502"
     CDS             20298..20654
                     /locus_tag="IWI97_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBC2463004.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20664..21563
                     /locus_tag="IWI97_001503"
     CDS             20664..21563
                     /locus_tag="IWI97_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBC2463005.1"
                     /translation="MGNLAIISDLHVDINKFGEPELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFANKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22080..22811
                     /locus_tag="IWI97_001504"
     CDS             22080..22811
                     /locus_tag="IWI97_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBC2463006.1"
                     /translation="MMKKEIVLTAATMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGPGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRGTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYMPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22956..24983
                     /locus_tag="IWI97_001505"
     CDS             22956..24983
                     /locus_tag="IWI97_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBC2463007.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISVVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENENLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDVEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            25091..26151
                     /locus_tag="IWI97_001506"
                     /pseudo
     CDS             25091..26151
                     /locus_tag="IWI97_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
BASE COUNT         8707 a         4559 c         5558 g         7327 t
ORIGIN      
        1 tttttatcct tcttttttta atctttttga cacgtaaaag tcatttttct gataattctg
       61 gcgataaaca gaaattatgt tgcaaagtaa agcggttttc tatataatta ttaataattc
      121 attacacaaa gataagaata acctttgtta taaaacgatt tattctgtgt aatgtccttt
      181 tttattaaga aaaacttcca tttaggaagc agagaggggt attcgattgt ccgataacca
      241 attattagaa gttcagaacc tacatacagg attccgactc aaagatgact attacgatgc
      301 agtagatgac gtttcattta ctttagacaa aaacgagatt ttagcaatcg ttggggaatc
      361 tgggtgcggg aaaagtacct tggctactac gattatggga ttacttgatc ctaataacac
      421 aaaaattaca ggtgagattt tatataacga tctgaattta atcgacctga acgaaacgct
      481 ctataataaa atccgcggaa atgatatcgg aatgattttc caagatccgt tgtctgcatt
      541 aaatcctctc atgagaatcg aagaccagat caaagaagga ctttcctatc acacaaagat
      601 gaatgcagaa cagcgtcaag ctcgtgcact agaattgcta gaacaagtag ggattcctaa
      661 tcctgcgaga gttggacgcc aatatcccca tgaactatca ggcggtatga gacaacgtgt
      721 catcatcgca atcgcaatcg cctgcaaacc tccaatcgtc attgcagatg aaccgacgac
      781 agccctagat gtaaccatcc aagcacagat tcttgattta ctgaaagacc ttcaggaaga
      841 aacaaaaaca gggatcatct tgatcactca tgacttagga gtcgtagcag aaatggctga
      901 tcgagtggca gtgatgtatg gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt
      961 tgatcatcct aagcatcctt atacacgctc tttactgaac tctataccac aagaaggaaa
     1021 tcatgatgca gaactccatg tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg
     1081 taaaggttgt cggtttgcac cacgtattcc atggatacca gcaagcgatc atgaagaaga
     1141 gcctatcctt catgaagtcg caccgaacca cttggtacgt tgctcctgct ataagcattt
     1201 ccactttaga gacgaaaaag gagaggtgta gatgacagaa ttaatcacaa tcaaagacct
     1261 gaaagttcat taccctattc gcagtggatt cttcaatcga gtaacggatc atgtatatgc
     1321 tgtagatggt gtggatttta ttatcgaaaa aggaaagaca tatggtttag tgggagagtc
     1381 tggctctgga aaatctacta cgggaaaagc cgttgtggga ttagaaaagg taacagccgg
     1441 acaaatcatg tatgaaggaa aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta
     1501 taacaaggat gtgcagatga tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg
     1561 agtgttggat atcattgctg aacccatccg aaattttgaa cggttaagcg atcaagaaga
     1621 aaagaaaaag gtaaaaggac tgctagatat cgttgggatg ccggaagatg cgctatataa
     1681 atatcctcat gaattttcag gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc
     1741 taccaatcca aaactgatcg tagccgatga acctgtatct gctctggatc tatctgttca
     1801 agcgcaagta ttgaatttca tgaaacgaat ccagcaagaa tacggcctca gctatttatt
     1861 catttcacat gatttaggtg tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa
     1921 aggtcgattt gtagaaatcg gtacaagaga agacatttat aatgacccgc gacatatcta
     1981 tacgaaacgt ctgctttcag ctattccgca aatcgatgta gaaaataggg aaagccacaa
     2041 agaaaatcga cgaagagtag aacaagagta tatccaaaac caaaaagaat actatgatgc
     2101 gactggtcga gtctacgatt tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga
     2161 tggaggtgca agctaatgtg gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa
     2221 gttattattt taagtgttct gatcttctta ttagcccaag cgatgccggg ggatccattt
     2281 accggtttga tcaatccaaa tcaggatccg gcagtgatcg aacagatgcg acaagcagct
     2341 ggcttgaatg acccatggta tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc
     2401 gattttggtc aaagtttctt atacaaacgc tctgttgctg acttgatcgg cgaacggatc
     2461 atcaacactt tatatctatc gatcttgaca gttgtcctta cttatttgat tgctgtgcct
     2521 ctggggatgc ttgctggccg gtatcaaaat tcaatcttgg ataagattgt cgtaatctac
     2581 aacttcttta gttttgctgt tcctttattt atcttcggct tgatcatgct atttgttttt
     2641 ggttatcgtt taggctggtt cccaacaagt ggatcacaaa cactaggttt gcaaggtgga
     2701 tttatcactc aatggataga ccgtttgaaa tatattctgt taccatcaat tacccaagca
     2761 tttttggcga ctgccgtcac aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg
     2821 ctcgattttg tcagaacagc ccgttccaaa ggggtaccga cgaataaagt attcagtcga
     2881 catattttcc gaaatgcagc gttgccgatg gcttctcaaa tggggtatga aatcacttcg
     2941 ttgattgctg gttctgtagt tatcgaaaag atttttggtt atccgggagt cggaaaacta
     3001 tttattgatt ccatcggcca acgggactat accgtgatca catccttagt attgatttta
     3061 ggaatcgcga cgctagtggg gacattactt tcagatatca tcatgagtat cgttgacccg
     3121 cgtattcgga ttcaataaaa ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca
     3181 atacagcagt tcaagaaagt attcctccta tggggatacg aatgattgct cgagaattta
     3241 aaaaagataa aattgctatt ttttcattag gtttgctagt ggttttatta ttagtgatct
     3301 ttatcggtgc gatgctgacc gatcaagaca aagtgatgta tgttagtatc ttcgacaaat
     3361 acgctgcacc aggaacagtt tcgacacaag gacaaaagtt cattctggga gcagatgaag
     3421 gcggacgtga tgtattagga caattgatca taggtgctag aaactctgtt acgatcggat
     3481 ttgcgatcac gatccttact tcgatcatcg gggttggatt agggatcatc tctggctact
     3541 acggagggat cattgacaac atcttgatgc ggatcgttga ttttatcatg attctaccga
     3601 tcatgctgat cattatcgtc ttcgtatcga ttatttccag atacaatgtc tggtccttta
     3661 tattcatcat gagtgcattt tattgggtag ctaaagcccg attattccga agcaagacat
     3721 tatctgaagc aagaagagat tatgtcagtg cctcaaaaac aatgggaacc aatgatttta
     3781 aaatcatgtt ccgcgaaatc atgccgaatc taagttcatt aatcattact aatttgacga
     3841 tgaactttgc ggcaaacatc ggtatcgaga caactttgac tttcttaggt ttcggcttgc
     3901 cggcaaatgt acctagttta ggtacattga tcggttatgc aagtaacgga gaagtcttag
     3961 tgaatcggca atggatctgg ttacccgcat caatccttat tttagttttg atgttgagta
     4021 tcaattatgt tggacaagca ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt
     4081 atagaagcga agggggaaat aggaatgaag aaaacgattt ttggatttgc agcgctgatg
     4141 tctgctgtcg ttcttgcagc atgcgggggc ggaggtacag atacctctgg aaacaactct
     4201 ggaggcaacg gcaataacaa agaggaatta acttttccaa ttgcaacaaa aaataatgat
     4261 gatgcaatcg aagacggcga attagaagct gcggtcgcaa cggacagtca gtttaaagga
     4321 ttgttccagt gggagtttta ccaagattca tatgatgcag cttttatggc tccttctcat
     4381 gaaccgctat ttgcaaacga tgacgatttc agaatcacag acgacggagc tgcaacgctt
     4441 gatctagatg aagatacgaa aaaagcgaca atcaaagtaa aagacaacgt caaatggtca
     4501 gatggtgaag atgtaacggc ggacgatgtg attttccctt acgaaatcat tgggaataaa
     4561 gactacactg gtatccgtta tgacgacact ttccgtaaca tcgtaggtat ggaagaatat
     4621 catgatggaa gtgccgacac aatctccgga atcaaaaaaa tagacgacaa aacagttgaa
     4681 atcgaatata aagaagtaaa cccaagcatg cttcaatcgg gtggtggtat ctggtcgtat
     4741 gcagcaccaa agcatacgtt agaaggtgta gcaatcaaag atatggaatc aagcgatcaa
     4801 gtacgtaaaa atccagtgac ttttggccct tattacatga gtaatatcgt tgcaggagaa
     4861 tcagtagaat acttgccaaa cgagcactac tggaatggta cgcctaaatt gaaaaagatc
     4921 acgatgaaat ctgtaccgat ggcaagtgca actgaagcat tgaactctaa attatatgat
     4981 atgattttcc aaatgccaac agatacttac gatacctata aagaaattgc tggctataca
     5041 aacttaggcc gtgaacaaac ttcttatact tatctaggat tcaagttagg taaatggaat
     5101 gcagatacaa agagtgtgga atacaatcca gattcaaaaa tggctgataa atcattacgt
     5161 caagcaatgg gctatgcact agataatgat gcagtaggtg aacgtttcta tgcaggtttg
     5221 agaagcaatg cgacttcatt gattcctcca gtattccagt cataccatga tgatgagatg
     5281 aaaggattca cacaagatct agacaaagcg aaaaaactat tagatgatgc tggctacaaa
     5341 gatgtggatg acgatggttt aagagaagac aaagatggaa ataaactaac aatcaacttt
     5401 gcttcgatgt ccggtgggga aacagcgcaa ccattggcag attactatat ccaacaatgg
     5461 aaagaaattg gtttggatgt ccaatacaca acaggtcgtc tgatcgaatt caattctttc
     5521 tatgataaat tagaaaacga tgatcctgaa atcgatgttt accaagctgc ttggaataca
     5581 ggaactgatc caaatccaag tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt
     5641 tatgaatcag aagaaaatac aaaattgatc aacgatatca gttcaagcaa agcatttgat
     5701 gaagagtatc aaaaagaagc atatcaaaaa tggcaacaat atgcgtttga agaagcattt
     5761 gcgattccaa cgttgttccg taatgaagta ttgccagtca acgaccgtgt caaagactgg
     5821 gattggtctt atgatgtacc aaacccatgg gcaaatgttt ctgttacagc agattcacgt
     5881 tcataatcac taaaaatgac cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt
     5941 tctgtttatt tctctaatat tcaggtcaag gcgctgtttc catgacttct ccaataaacg
     6001 gtgttcaaag tccagtttct cggtattaag tcaaatttat gcaaaaacat ggaaagtcat
     6061 ttttacaaaa ttttttcttc atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa
     6121 tcactaggat tattaaagct ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa
     6181 aaccagtata ataaggaagt atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca
     6241 accagctgac aaaagaacta aaagaaaact ataacatcgt ttttcacgat ctaaatttac
     6301 ttgaacaagc ttttacccat tcatcctatg tgaatgagca tcgcaatctg caattatcgg
     6361 ataatgagcg acttgaattt ttaggagatg ccgttttaga actgatggtt tctcagtatt
     6421 tattccgttt atatcctcat ttgccagaag gaaaattgac aaaaacacga gctgcgatcg
     6481 ttcgcgaaga cagcttgtcg aaatttgcca aagaatgtca ctttgatcaa tatgttctgt
     6541 tgggcaaggg agaagagaat tcaggaggac gtacgcgacc agcattgctt tgcgacctat
     6601 tcgaagcttt tttaggcgcg ttatatcttg accagggatt cgacacgacc catgcattca
     6661 tcgaaaaagt gatttttcct aaagtcaaag caggtgcttt ttcacatgag atggatcata
     6721 aaacaaaact acaagaagtc ttgcaaaaat caggtgatgt atcaatcgaa taccgattga
     6781 tcaatgaaga aggtccagct catgaacgag tattttggat cgaagtttat gtagatgatc
     6841 aattgattgg cactggacaa ggaaaatcga aaaaattagc agaacaagct gcggcggaaa
     6901 atgcattggc agcactgtaa aagtaagtga gggaaagctt tgtatttgaa acgaattgaa
     6961 atagccggtt ttaaatcatt tgcagacaag acagtcattg attttgaaaa tagcgtcaca
     7021 gcagtggttg gaccaaatgg cagtggaaaa agcaatatta cagaagctat ccgctgggta
     7081 ttaggagaac aatctgctaa aagtctgcgg ggcggaaaaa tgccggatat catctttgcc
     7141 ggttccgata cccgtaagca gctgaatatt gcagaagtca cagtgatttt agacaatagt
     7201 gatcattatt tgccattgga gtacaatgag atcagtgtaa cccgccgtta tcgacggact
     7261 ggggaaagcg agtttttcat caataaacaa agctgcagat taaaagatat ccaagaattg
     7321 ttcttagatt ctggattagg aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa
     7381 gcgattttca gtagtaaacc ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc
     7441 ttgaaataca agcagcggaa aaagaaagca gaacagaagc tttttgaaac agaagacaac
     7501 ctcagccgcg tccaagacat catccacgaa ttggaagaac agttaactcc tttggccgcc
     7561 cagagtgagg cagcaaagga gtttttacgg ctaaaagaaa cactgactca aaccgatgtc
     7621 tctttgatgg ttgccgaaat caagacagca aaaaaagatt gggacaacaa acaagcacag
     7681 cttgcgaaat tcaatctaga attaggcaaa ctatctgagt cgatccaaga acaagaatcc
     7741 attttggcga agcagcgtaa agaaaatgcg caagccgacc gtctgatcga aaaaaatcag
     7801 caagtattat tagatctttc ggaaaaactg aagcagacag aaggacaaaa agatgttttg
     7861 caggagcgga caaagcatac acaaaaaagc tcgcaagaat atcaaacttc attagcagaa
     7921 gcacaaaaaa aggttaaaca ttttgaaaag cttcaagaaa gcttgttgaa agaagcagca
     7981 gaaaaagaaa cagagatcca aaaagctgaa gcaaatctca taaaaacaca gcaagaatta
     8041 gaaaaatacc aaaaatcaac gaaagaatta ttggcagaat taagagatca atatgttgat
     8101 ttgatgcaag aacaagcagc tgtaggaaac gaactgaagt atctagaacg tcaatatata
     8161 caagaaacag caaaaagcaa gcagacgtta gccaagcaat cggaagtaga agcatcagta
     8221 gaccgattga tcttacaaaa agaagaattg acacaaaagc aggcgcagct aaaatcatct
     8281 ttaactgaaa caaaagagaa gttagaaatg atccaacaaa acgggaaaaa gttccaagaa
     8341 gtattagcaa aagaacagcc taaaatgtat caattgatga atcaagtcca gcagctgcgt
     8401 gctcgtcaga aaagccttca agaaatccaa gaaaactatt ttggttttta ccaaggggta
     8461 cgattggtct tgcagcataa acagcagctt tcgggtattg tcggagcagt ggctgaactg
     8521 atcgatgttc ctgcagattt cacgttagct atcgaaacag ctttaggtgg ggcagcccag
     8581 catgtcatcg ttgaaaatga aaaagatgct cggcaagcca tcacgtattt gaaacagcaa
     8641 agaggaggaa gagcaacatt ccttccactg acgacaatca aaccaagaca attaccggct
     8701 catatattga cacaagcagc agctgtagaa gggtttatag ggattgccag tgaacaagtc
     8761 agttatcccg atcaaatcca aactgtggtt caaaacctgc taggaacgat ccttttagcc
     8821 aaagacttaa ctagtgccaa tgcaatcgcg caaacgatcc gctatcaata tcgagtggtt
     8881 tccttagaag gtgatgtgat gaatgccgga ggatccatga caggtggagc gaataaacga
     8941 ggaaaccaag gcagtttgtt tgtacaaaat caagaattga aacagttgac tagtgaattt
     9001 gaagaagcag ataaacaact ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa
     9061 acagctcgtt tagccgaaga acaagaagtt ttgcgtacac gaggcgaaca attacgtttt
     9121 gaagaacaag aagcgacaaa tcagctgcaa aacatcataa atgaacttga acgttttgaa
     9181 aaagaaaagc aaatctcaac ttttgagaca cgtgaactgc agcagtttat cgaagattac
     9241 gaaaagcaaa cgaacgaatt gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa
     9301 atcgacgaag aaatcaaatc gttaagtcaa gaaagcgacc agatggaagc cagaagagca
     9361 caagtacaaa gtcaaaaagc acaagaacaa gcagatcttg ctgtcttgaa agagcagttt
     9421 aatcatctgc agatccagct ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt
     9481 caagaagcga tagaaaagca gttggcgaca ttgacagcag acttttctga tcatgaagtc
     9541 acagaagaaa gtttggaaaa acaaattaac gaattatctg ctcagcgaga aacattaaaa
     9601 gccgagttgg caaaagccaa agagcagcga gatagaaggc aaaaggagat cgataagctt
     9661 gaagcagtct tggcagagcg taatcgtgag caaaaagcac gactgtcgga acaatccaaa
     9721 ttagaagtcc aaaaagaccg agctgaaatg attttagata atcatttgac ctatctgcag
     9781 tcagaatatc agctgacatt tgagaaagct tcccaagact atcaagaaac aaccgatatc
     9841 gaagacagta gaacaaaggt ttcatcctta aaagaacaga ttgaaaaact gggaccagta
     9901 aacttaaatt caatcgaaca atacgaacaa gtcagtgaaa gacatacatt cttagcaaca
     9961 cagcgagatg atctgttagc agcgaaaaac caattgtttg aaacgatgga tgagatggat
    10021 gatgaagtgc ggacaagatt caaagaagta tttgaagcta tccgtcaaga attcaaaatt
    10081 gtctttccaa atatgtttgg cggcggtaaa gctgaattag tactgacaga accaactgat
    10141 ttgctgaaaa caggtattga gattgaagta caacctcctg gcaaaaaact gcaaagtcta
    10201 agtttgcttt ctggaggaga acgagcactt acagccatcg cgctgctctt ttctattatc
    10261 cgtgtctgtt ctgttccgtt ttgcatctta gatgaggtag aagcagcgtt agatgaagca
    10321 aatgtgaaac gttttggtcg ttacttgagt gatttccaag atgacacaca atttatcgta
    10381 gtcacacacc gcaaaggaac gatggtcgca gcagatgtac tgtacggagt gacgatgcag
    10441 gaatccggtg tatcaaaaat cgtttctgta cggatggaag atatcaatga agaggcgaaa
    10501 atcaacgaaa cgaaagtgta ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg
    10561 gtacattatt aaatgaacaa aagctgattt cagatgagaa taaacaagca ttagcacaag
    10621 caaaacagca aggggtcaag atcgttctat gtacaggacg ccccttagct gcgatggcgc
    10681 attatctgca agaattagga ctggtagatg aaggtgacta cagcatcact ttcaacggcg
    10741 gtttggtaca aaaaaatgat actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag
    10801 atatccaacg cttatacaaa ctggctcagc aattggatct gccactagat gttttgtctg
    10861 acaatgtggt tttacagctg cctagtgccc caaggaagca atccttgtac aatgtgttga
    10921 ataagctgct gcaattccaa ccagcggata taagcgatat tacagatgaa tttgtgctta
    10981 ataaggcagt aatcgcttat gaccaagaag aattagatcc taaaatcaaa gaaataccag
    11041 cagaattcca tgaaatgtat gagatcatca aaacgagaag tatgcttttg gaatttatgc
    11101 caaaaggtgt tacaaaagct tatggtattt ctttattggc aaaagatctt ggtttgaaac
    11161 aagaagaaat catggcaatc ggtgatgaag aaaatgattt accgatgatc caatacgctg
    11221 gactaggcgt agcgatggag aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga
    11281 cagccagcaa tgaagaaaat ggcgtagcca aagtgatcca aaaatttgta ttagagtagg
    11341 ggggagtcaa atgggatttt ttgataaaat caagcgtgct tttactggcg aagagaaaac
    11401 agaagaagtt gaagtacagg agaagtacga caagggttta gaaaagacgc gtaaaacatt
    11461 tggtgaacgg atgaatgagt tgtttgccaa ttttcgaaca gtcgatgaag acttttttga
    11521 agaattagaa gaaacactta tcggtgcaga tgtaggtttt gaaacagcga tacgaatcac
    11581 cgaagcatta cgtcaagaag tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa
    11641 tgcgatcatc gagaaacttg tggatctcta tgaagcggaa ggaatcaatg aagtaaatga
    11701 actaaatatc caagaagacg gactaacagt tattttgttt gtcggtgtta atggtgtagg
    11761 gaaaacaaca agtattggta aacttgccca tcagttcaaa aacgaaggta aaaaagtatt
    11821 gatggcagca gcagatacct tccgcgctgg agcaatcgac cagttagttg tctggggcga
    11881 acgcgctggt gttgaagttg ttcgtggcaa tgcaggcggc gatccagcag ctgtcgtatt
    11941 cgatgctttg gaaagagcaa agacagagca ggcagatgtt ttactagttg atactgcagg
    12001 ccgactgcaa aataaagtga acttgatgaa cgaattagaa aaaataaaac gagtaatcca
    12061 gcgggaatta ccagaagcac cccaagaaac acttcttgta ctagatgcaa caactggtca
    12121 aaacgcgatg gttcaagcaa aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt
    12181 gacaaaatta gatggtacag caaagggcgg tatcgtactt gctatccgta atgaattgca
    12241 tttaccagtg aaattggtcg gtcttggtga aggaatcgat gacttggagc cgtttgatcc
    12301 aaatgatttt gtcgttggat tgttcaaagg attactaaaa gaagaataac ccatgtctaa
    12361 aaagcaggag ctgtgacaaa agttgcagct ccttgtttga atgattgttt ttaacatagc
    12421 tgtgtttttg atgccttcat ttttatactt atttgtaagc ggttctcaaa caatttcgtt
    12481 ctttttctta gactttttga ttataattag attcatatga attggaggtc acttaatgat
    12541 gaattttaag caattattat tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt
    12601 tggtatagaa cgggaaggac agcgggttga tcttgcagga aatctagcaa aaaccgatca
    12661 tccagcaatc tttggcgatc gatcctatca cccctatatc caaacagatt ttagcgaaac
    12721 acaaacagag atgatcaccc ctgttaccga ttctattccc gaattatttc agtatctcgc
    12781 tgctgtttat gatgtgactg ctcgttctat accgaaagaa gagatgatct ggccattaag
    12841 tatgccacct gccttaccgg aaaaagacga agagatcatt attgcaaaat taaaaaattt
    12901 cgaagatgtc ttgtatcgac gttatttagc aaaagaatac gggaagcgaa aacagatggt
    12961 aagcggtatc catttcaatt ttgaatttgg cgatgagttg ctaagaacat tgttcagcca
    13021 tcaggaagaa tttcaagatt tttccgaatt caaaacagaa ctttatttga aaacagggag
    13081 aaactttatg cgctatcgct ggatgatcac ttatttattc ggtgcttcac caatgagtga
    13141 gaagaactac tttttagatg aatcacatcc gcaagaacct gttcgcagta ttcgcaacag
    13201 tgcgctgggc tatacgaatc atccaaatgt gaaagtttcc tatgcttcta tgaaacagta
    13261 tttagcagat atcgagcgaa tgatcgaaga agggaaactt tcggaggaga aagaatttta
    13321 tacaccgctt cgtttccgag gtggaaagaa agtcgcagat ttagcaacaa caggtgttcg
    13381 ttatattgaa ttgcggaata tcgatttgaa tccttatgca agattgggta tcaatccaga
    13441 gcaagttcgg ttcttacaat tattcctgat gtacatgtta tggacagaag aaaaagaaga
    13501 ctgtgaccaa tgggtagcag aaggtacgac tcgaaataac aaagtagcac tagaacaacc
    13561 atcagatcaa acagaatttc atcaagaagg tagagaaatt cttgaaggga tgaagcaaat
    13621 gctggttgaa ttggattggt tggattctct ttatttagta gaggaagcat tgactcaaat
    13681 ggatcatcct gaacaaacgt tagcagcgaa actctatcaa gaagcacagc tatcgagtca
    13741 gcaagaagtt gctgtggcat tgggacatca atattataaa gaaagccatg aacgtcccta
    13801 tcagttggcc ggttttcgcg aaatggaact ttctactcag atatttatgt tcgatgccat
    13861 ccaaaaaggt gtccaagtca aagtattaga tgagtcggat caatttttgc gtctgcaatt
    13921 ccaagaccat gtagaatatg tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt
    13981 cccacttatc atggaaaaca aaacagtaac aaaaaaagtc ttaaaagaag ctgggttccg
    14041 ggtaccagga ggcgcagaat tttcatcaat ggaagaagcc gtaaaagctt atccgagatt
    14101 tgccgatcag gcatttgtga tcaaacctaa atcaaccaat tacgggttag gtatcacgat
    14161 tttcaaagaa ggagctagtc tggaagatta tcaagcaggt ttagccattg cttttcgtga
    14221 agacagttcg gttttagtag aagaatttat gccgggaacg gaatatcgtt tctttgtgat
    14281 cgacggagaa gtccaagcca tcatgttgcg agtccctgct aatgttattg gtgacggtat
    14341 ccgaacagtg aaagaactag ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca
    14401 tcgtgcacct ttagaattga tccaattagg tgaattagaa caattgatgt tgaaagaaca
    14461 agggttaacg atcgaatcag ttccccaagc caatcaaatt gtttatctaa gggagaactc
    14521 aaatatcagt acgggcggtg attcgatcga tatgactgat gaattttctg aagcttacaa
    14581 aaaaatcgcc gtttctgcgg tagaagcact aggagctaag atcagtggta tcgatttgat
    14641 tataccagat aaagaaatcg atcctacaac tgataaaaaa gcatatggga tcatcgaagc
    14701 aaatttcaat ccagcgatgc atatgcatgt ttatcccttt gcaggaaaag gcagaagatt
    14761 gacgatgaat gtattgaaat tgttatatcc tgaagtattt taaaagaaaa agcttttgac
    14821 agattgtcct atttgatgtc aatctgatca aaagcttttt ttgcatgtaa aaatttatgt
    14881 gttttttcgt tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt
    14941 tcagctggcg aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt
    15001 actgcttctt ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg
    15061 ttgactacag taaatcggct attgtttttc gttgctagct ggatatattg gttttggaca
    15121 gaaaggggta gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt
    15181 aaaaagtttt ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag
    15241 cgttccctga atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca
    15301 taaagtccct tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt
    15361 cttctctttg tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa
    15421 aagcggttgc ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag
    15481 aaaggtggat tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat
    15541 ttcattgttc atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca
    15601 aagcttacaa atcttgctag cgactcgttt gccacgaacg atttgtttga tccttgtggg
    15661 agcgaccagt agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc
    15721 accaacgact gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt
    15781 cctacctggt gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg
    15841 aacattgctt tttttatccc ttacgcgatt gttcccccag aaaaatcaat tgatgcttcc
    15901 gttgattgga gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca
    15961 attccagttg gtacaaaata tgacttcatg gcttcaaggg aattttgcga ctgtgatacg
    16021 aggaaattat gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc
    16081 ttaccgattt acgatcattg gtctgggcga atcgtttgca gtaaatttgg gaatagatta
    16141 ccagaagatg cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat
    16201 catggtagga agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata
    16261 tggagatcaa gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt
    16321 attttgcgat cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat
    16381 cgttggaatc atcggtgggg ctatcttcct ttatcttctg ataagggggc gacgatatgc
    16441 ttaaaaaaaa gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa
    16501 tgtacttgac gatccataca tatggaaact ggcattttgc atgggcattg aggagtaaga
    16561 aaatttttgc atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga
    16621 caattacgca taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc
    16681 ttgtccaaac ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta
    16741 caggtttttt cttattgaac gttgggctga tggtcgggtt gagtctgttg ttatttttct
    16801 ttcttttgaa aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag
    16861 gtacgctttt tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg
    16921 atttgcttca aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc
    16981 ctgcttcttt gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag
    17041 acgttttcca tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg
    17101 aatttttgat tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc
    17161 cagttacttt tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc
    17221 gtcatggtca gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac
    17281 agctcgcagt agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg
    17341 tcggaggaat ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga
    17401 tttacaaaag gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac
    17461 gattcctgaa aagaagttga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt
    17521 gttgtcgatg atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa
    17581 cgaagtaaag gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc
    17641 gaatgaaata aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta
    17701 tacgaaagga aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacct
    17761 tgggttagaa gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg
    17821 tgcgtatatt gcaatggtac tagcgcaaga cacggattat attttactgg atgaaccttt
    17881 gaacaatcta gatatgaatt ttgcggttca aatcatgcag atcttaaaaa aattagtaca
    17941 agaaatcggg aaaacaattg ttatcgttct tcatgatatc aactttgctg caagttatgc
    18001 agacgaaatt gttgcgatga aagatggaca gttattcacg caaggaacga cccaagaaat
    18061 gataaaaaaa gatgtattgg atgaattata cgaaatggac atacggattt gtgagattga
    18121 aggccgcaga ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat
    18181 tagtagtagg tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagcaaca
    18241 acaccagtca ggcagataca aagaaaagca gtacgcaagc tgaaacggca ttaacgataa
    18301 aagacagtaa tggcgatcaa atcgaggtgc caaagaatcc tgaaaaagtc gtcgtattcg
    18361 acaatggttc tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag
    18421 ctacgagcag ccttcctgag tatctttcct cctataaaaa agtggaatca gcaggtggga
    18481 tcaaagaacc agacttagaa aaaatcaatc agcttcagcc aaacctgatc atcatttctg
    18541 gccggcaaag agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt
    18601 tagatgcgaa aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct
    18661 ttggtaagca agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga
    18721 cgaaaaagaa agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc
    18781 aattatcagc ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg
    18841 aacaagcaga tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg
    18901 tgctggaaaa aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag
    18961 acgacagcaa agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa
    19021 atcagcagat catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat
    19081 caatgaaact gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga
    19141 ttaaactctc atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat
    19201 cctcaaagaa gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt
    19261 ttatttattt gggaaatctt ttttcaacgt gaattcattc tctacatcat acgggttgat
    19321 tctagaatgg ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc
    19381 ctttgatttg aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt
    19441 tattttgtac cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc
    19501 cactagacta gaaaatatga gaagctattt tctgtccagc gttcttatta cttaaagcaa
    19561 atcgtagttg tcaaaatctc ttctaaacga tgttccatca gctgaccttc ggtattagct
    19621 caaagcactg atttcacaac ctctatttag gcatgtgtct cagcaagcgg ttgatagttg
    19681 atgatctgca tttttcgata aaaatcggcg ggaagcttac tccaccgttt taataattga
    19741 tagaaataac tttcttctgc tttgatttta tactcgctta atatcaatcc aaaactattc
    19801 gttttttttg aagctaattt tttataaata tccaatgctt tggttttatc ttcattgaca
    19861 aagagtgttt ttacttcaat ataagaaccc tcgatatttt gtgtttcttc gataaaatag
    19921 atgtttttta tatattcctt catagtgatt cctccaaaga agtcttttat ttgataagtt
    19981 tactctaccg aagagttgtt aataatccgt gaacaaaaag taattctttt tccaatagtt
    20041 gttaaaggtt ttttattttt tatagtattt tttgttattt aagaaaggaa taaggttaaa
    20101 caaataacag gttgcttttt gggtcttttt ccgttcaaga aactgaacga agaaaaggga
    20161 aagttgtctc ttttgtgata agagaaatca taataaacga atgacttact ttccaataaa
    20221 ttgagaacag tcagaaccag acaaattagt tgcatctgat gaataaagtg attattctat
    20281 gacatgaggt gatacagatg aaattagaag tgacaaaaga agcacaagaa gtattgaaga
    20341 ataaattaga agcagacgat caacttttat tagatatcga aaacggtgac ggtccatttg
    20401 cagaaagcca agtcagttgt caattagaca cggcatttcg attgatcatt gttaaaaaag
    20461 atacacaaac agatctaagt ctttattctg tagtggctga tacaccagtt ggaccaatca
    20521 aaatcaaaga cagtgcaata ctttacatgg atgatccatc cacacttgct ttagaaccaa
    20581 cttataatag tttacaatta aaaggaccaa gtggcttgag aaaaggcaac ttgcaagttg
    20641 ttcgaaaaga ctagagggat aaaatgggaa atttggcaat catcagcgac ctacatgtgg
    20701 acatcaataa attcggcgaa ccagagctaa tgcagctttg gcaagttttg caaaagcaca
    20761 aaatcactcg gcttcatcta gccggagata cggctaataa agtagatcgc tgcctagcgg
    20821 ttgtcgaatt tttcgccaat aaaggattat ctacgacatt tcatttcggc aatcacgagt
    20881 tggcagatgt gcccaatgaa cagatgatgg accattttcc agacccacat tttttaaatg
    20941 aacggtatat tgctttaaat gagcagaccg ttcttttagg gatgaatggc tggtacgact
    21001 atcagttttc cgaattgaaa gattacgagg caatcgtacg aatgaagcgt ttgtattggt
    21061 atgatcggtt catccagcgt gggaaaacag atctcgaggt caatgcagaa atgcttgcag
    21121 ctgcaaaaaa tttgctagac cagctggaag caaaaaaact tgaggtgatt ttatccactc
    21181 atttcgttcc taaacgggca tttatcatgt atcaatctgc tccttatgag cgttggaaca
    21241 aattaaatgc atttttagga tcagaatcat tcggagaatt acttgaccgt tattcaaata
    21301 tcaaacaagt agtttttggc catacgcatc gtcgctttga agataaatcc atcggtcaca
    21361 cgatttacag ctgcagacct tttggttatt attatgaatg gcagctgacc cgagattttg
    21421 tcttgaaaga acagttgcta gacagctatg atcctatgaa attacgtgtc ctcttgcgtg
    21481 cccatcaccc agcttttcag gcctatcaaa cacaacattt gcaagaggaa tttgaacaag
    21541 cgatgacaat cgttccgtat tgagacagca ttagtaatgt gtaaaagaaa ataccttgta
    21601 aaacataagg ataagtaaga gcagtagatc catttatcgt ggatatgctg ctttttttgt
    21661 ggaaaataat cagcgaatgt ttatttgatc ggatgatttg taactcgcag agtactagat
    21721 gcaagcgttt attctttttt tgaacgataa tcacaaaaaa aaatataata tacatttttt
    21781 atttaattat aattaatatt gattgaagtc taggcagtca agtcacattt tgatagatag
    21841 gagggggact tacgaattat tttaaaggct atgacagctg tatttctaga ttgtgtttgc
    21901 atcaagttgc aataaaaact agaaatcaaa tcgggctgat aatcatttat aactaatcga
    21961 tacataggac caaatatagg tctatgttct tttatttcat aagtatcgta aaacagaatt
    22021 ttgctatgga ttaatccaat cgactgatat gtttagtaaa taaagaaaga ggaatgtcaa
    22081 tgatgaaaaa agaaatcgta ttaacagcag caacgatgtt attcagcatg gtagcaagta
    22141 cgacttttgt ttctgcaact gaagtatacc caaaggaata taacacagaa ggaacgatta
    22201 catttgaagc aggagatgag ggtgtaactc ctcctgttga tccagagaat cctgatccca
    22261 ataaacctgt cgatccatca gatcctccaa gccctggacc aggaggggca ttatcgattg
    22321 attatggatc aaaattcaaa tttgggactc aaaaaatttc tactgcggat aaaacgtatt
    22381 atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc gacatatgtc caagtgacag
    22441 ataggagagg taccttatct gggtggaaat taagtgtctc tcaaccagaa cagtttaaaa
    22501 ctgccagtgg ggatgaacta gttggagcac agctgaaatt tacgaaaggg caggctgttt
    22561 ctttggttga tccaacatat atgccgcaaa cggtgaattc tgaattaacg ttgacaccgg
    22621 gaggaaacaa tacgttagca attaatgcta aatcaggaac aggtgtgggg acttgggtct
    22681 atcgatttgg tgcaaatgaa aatgaaaatc aagatgcggt acagctttcg gtacccggta
    22741 aatctgtcaa gttagcgcaa caatattcca ctaaattagt ttggacatta gaagatacac
    22801 ctaacaacta gtttactgaa aagaactgtt aaaaataaat acgtagtcat attgtcttgc
    22861 gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt tgtttggata cgaatagttc
    22921 atctaggaat ttaacgatca ggaggaaaag atatattgaa aaagaggagg atcgtgctca
    22981 ttttaagcag tctcttagtg actaatatct ttccgatcat agggtactct ttggaattga
    23041 atcagttgga gcatggaaat agaagagacg aagaaagcaa actcacttcg acattcgaca
    23101 gtttaataga atccaaccag acacaagaaa gcagtgagga tttaagtatt tcgagtagtg
    23161 gagagaccac tgacaacgta gaagctgagg gttcggacaa acaaggactg gaagatgcac
    23221 caatttctgt ggttgagctg cctgacgatc ccaatattat ttctatcgat aaaatttttc
    23281 aaacacctat cggtactagc acgtccatat tggaaaatgg gaagttactg cagttgaacc
    23341 cagcagaaag gtctcaaaga ggagctattt ggtcaaataa gaagattagt ttactctcgg
    23401 attttacatt taaatcttat ttatacttag gaaatcaacg agatagagcg ggagatggca
    23461 tgacctttac attgactggc gatcctagaa tggctagtaa tcccagtcaa gttattggat
    23521 cgccaggtat gggaattggc tcatattcta ctcgttctgg ccaaccttac attgcgaatg
    23581 cgctctcgat tgagtttgat acgtataaaa atagtggttc ttctgatcga atggatcgag
    23641 agataactgc tgataataga gggcatggac atgtcgcttt tgtcacgcct aaaccaaata
    23701 ataataatta ttctggagag catactggcg tgacggtagc tcctacattt ctttctaatg
    23761 gcacatggcg cttgttaata attaaatggg acgcggagca aagaagtctt acttatgatt
    23821 tggaaggtgt cggtcgctct tctcggatta tacctgatct cattaggcag tttggttcta
    23881 cagaagtaaa ttgggggttt acttcttcaa caggtacttt ttttcaagaa aatgctgtcg
    23941 ctgtcacgcg acttccatcg aatgtcacat caacagctga tgtaaaagtc aacgatagtg
    24001 attatggggc catcgctgaa gctggaggaa acgataagat aactatcaga aatacattga
    24061 aggtcgaggg ggattttctt gaatataaga aaatgcagac tagtatcgac ctcccacaag
    24121 aattaactat tcccgagcat atcttgatca atggaaatca aattgatgcc aaagatatct
    24181 tgataaccgg aaatcgagtc acgctaaatc tgacagatta ttttattgct ggagatacgt
    24241 taataatcga attggagaca actttgcagg aagtaattcc tgaaaaagta ctcactatgc
    24301 attttgaata ccttgaaaac gagaatttga ttgagcaatc gaatgaaata aacctaaaga
    24361 tcgcaaagct gaaggagata gatatccatg tttattataa agataaagac agtcagattg
    24421 atattgctga aatgaaaaca atcactggga aaataggaga gaagtataaa gaaactccta
    24481 ttgatgttga agggtatgtc tttgactcgg attcagcgaa tacagaaggc atcttctccg
    24541 aaaatacaga ggatatctat ttttattaca gaaaaggcaa actttatctc tcaactgctc
    24601 ctcaatatgt caattttgga aaacataaaa tatccagtag accattaagt gaattcggac
    24661 agttttcagg tgggttgaaa attatagatg aacgggcttt tggaagttgg aaattacaat
    24721 tgaaacaaga gaaagttctt acaaatggag attttgagct tccagaagct ctttcctttg
    24781 ttacctctga aaatacaacg gttataggga catccgctgt tacgattttc gaaagtgatc
    24841 aaaagggtga aagcgatttg tcctctcttt tagattctac agctcatcgt ggaatcagaa
    24901 tagatattcc tgtagaaaac caaagaatcg gaacctttga ggggaaacta tcttggatac
    24961 ttgcagatgt cccaggaaat taacaggaag agaagaatat aacaaaatta ttgagtttca
    25021 agaagggcac aggtaccata ggctcttgtg tcccacagag acaagatttt gttatagtct
    25081 tctcttacat atgattaaag gagcgatttt cttgagacca aaacaacaat tatttataac
    25141 actcctagtt attttagggg cactttttaa caatatatca gtgtcagcta gtgcatttaa
    25201 tttttcagtg acgccgataa cttcagagaa tcaaatcgac aagagaaaaa cttacttcga
    25261 tcttcaactt gatccggatc aggaagtaga agtaaaagcg gaattgagaa atgatacaga
    25321 aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc accaattcaa atgtgatggt
    25381 tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg atctttgatc tggtagatta
    25441 tgtgagctat ccacagatag tgacgctaaa acctaaaagt gtccaaaccg ttgcttttca
    25501 tgtcaggatg ccgaataagc gatttgatgg tgtattggct ggtgggatca cattccaaga
    25561 aatccagacg gaaaaggacc aaacagaaac taaggatcaa agcttatcga ttgagaatga
    25621 atacacctat attattgctt tgttgatgcg acagacactg aacgaagttg ctccgaacct
    25681 tactcttcat taagttaaac cagatcaaat caatgcaaga aatgtcatct tagcaaacgt
    25741 tcaaaatgat caaaaaacct atatcaatca agtagttatt gaaacaaaaa ttacaaaaaa
    25801 aggacattct gaagttttat atcaagagga aaaagaagcg ttgcagattg cgccgaatac
    25861 aaatttttct tttccaactg ctttaaatgg tcaaccgcta accccaggtg aatatcacct
    25921 tacaatgact gttttaggaa atgaaaatga aaatggaaaa ttttcgagaa aaaaaggaaa
    25981 tactacgatc aactatacca atcaatgggt atttgaaaaa gattttacaa tagacgaaaa
    26041 agtagccaag gaattgaaca cgacggatgt tacgattaaa aaagacaata attggattta
    26101 tatcttagta gcttcacttt tcttattgtt tattttatta tgcatacttt t
//
LOCUS       DADXTH010000038        26036 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.038, whole genome shotgun sequence.
ACCESSION   DADXTH010000038 DADXTH010000000
VERSION     DADXTH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.038"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(179..586)
                     /locus_tag="IWI97_001507"
     CDS             complement(179..586)
                     /locus_tag="IWI97_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBC2463008.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(581..1785)
                     /locus_tag="IWI97_001508"
                     /pseudo
     CDS             complement(581..1785)
                     /locus_tag="IWI97_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1911..2129
                     /locus_tag="IWI97_001509"
     CDS             1911..2129
                     /locus_tag="IWI97_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463009.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2150..2785
                     /locus_tag="IWI97_001510"
     CDS             2150..2785
                     /locus_tag="IWI97_001510"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2463010.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2978..3217)
                     /locus_tag="IWI97_001511"
     CDS             complement(2978..3217)
                     /locus_tag="IWI97_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463011.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3241..4764)
                     /locus_tag="IWI97_001512"
     CDS             complement(3241..4764)
                     /locus_tag="IWI97_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBC2463012.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4782..4904)
                     /locus_tag="IWI97_001513"
     CDS             complement(4782..4904)
                     /locus_tag="IWI97_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBC2463013.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4934..5917)
                     /locus_tag="IWI97_001514"
     CDS             complement(4934..5917)
                     /locus_tag="IWI97_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBC2463014.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWI97_001515"
     CDS             complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWI97_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBC2463015.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6111..6488)
                     /locus_tag="IWI97_001516"
     CDS             complement(6111..6488)
                     /locus_tag="IWI97_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463016.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWI97_001517"
     CDS             complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWI97_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBC2463017.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWI97_001518"
     CDS             complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWI97_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBC2463018.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7506..9272)
                     /locus_tag="IWI97_001519"
     CDS             complement(7506..9272)
                     /locus_tag="IWI97_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463019.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSRTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9232..10986)
                     /locus_tag="IWI97_001520"
     CDS             complement(9232..10986)
                     /locus_tag="IWI97_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463020.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11096..11665)
                     /locus_tag="IWI97_001521"
     CDS             complement(11096..11665)
                     /locus_tag="IWI97_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBC2463021.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11683..13098)
                     /locus_tag="IWI97_001522"
     CDS             complement(11683..13098)
                     /locus_tag="IWI97_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBC2463022.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(13109..13777)
                     /locus_tag="IWI97_001523"
     CDS             complement(13109..13777)
                     /locus_tag="IWI97_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBC2463023.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(13908..14492)
                     /locus_tag="IWI97_001524"
     CDS             complement(13908..14492)
                     /locus_tag="IWI97_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2463024.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(14823..15056)
                     /locus_tag="IWI97_001525"
     CDS             complement(14823..15056)
                     /locus_tag="IWI97_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBC2463025.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(15071..16018)
                     /locus_tag="IWI97_001526"
     CDS             complement(15071..16018)
                     /locus_tag="IWI97_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBC2463026.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16018..16860)
                     /locus_tag="IWI97_001527"
     CDS             complement(16018..16860)
                     /locus_tag="IWI97_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBC2463027.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(16993..17718)
                     /locus_tag="IWI97_001528"
     CDS             complement(16993..17718)
                     /locus_tag="IWI97_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBC2463028.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(18179..18493)
                     /locus_tag="IWI97_001529"
     CDS             complement(18179..18493)
                     /locus_tag="IWI97_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBC2463029.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(18509..20104)
                     /locus_tag="IWI97_001530"
     CDS             complement(18509..20104)
                     /locus_tag="IWI97_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBC2463030.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(20127..21017)
                     /gene="iolE"
                     /locus_tag="IWI97_001531"
     CDS             complement(20127..21017)
                     /gene="iolE"
                     /locus_tag="IWI97_001531"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HBC2463031.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(21034..22059)
                     /gene="iolG"
                     /locus_tag="IWI97_001532"
     CDS             complement(21034..22059)
                     /gene="iolG"
                     /locus_tag="IWI97_001532"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HBC2463032.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(22083..23090)
                     /locus_tag="IWI97_001533"
     CDS             complement(22083..23090)
                     /locus_tag="IWI97_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2463033.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(23203..24363)
                     /locus_tag="IWI97_001534"
     CDS             complement(23203..24363)
                     /locus_tag="IWI97_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBC2463034.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(24439..25893)
                     /locus_tag="IWI97_001535"
     CDS             complement(24439..25893)
                     /locus_tag="IWI97_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBC2463035.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         7989 a         5314 c         3995 g         8738 t
ORIGIN      
        1 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
       61 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
      121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
      181 agtaaatgta gtaatctttg ctttcttttt tctcaatact aataactaac ttatggggta
      241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
      301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
      361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
      421 tttccttgcc atcaatttga acgatggcat atttgatagc atcttcagtt gtttcattct
      481 ccttttctat agagaataca aataagggaa taaaagaggg aataatcaaa atgccaataa
      541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
      601 taaacatatt agaatttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
      661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
      721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
      781 cgggtttttt tgtataatca tttgacactc aattttaggt aaactagcta cccaataatt
      841 taatgattca atacttttat ggctacccga tgtttcaaag atcgtcgaaa aatcactatg
      901 ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat ttttatccgt
      961 aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa aacttgagat
     1021 accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact ctttgaacca
     1081 gcccattgaa taggaagtct catctatatt aaaaagattt gtatttagaa ttttcgataa
     1141 gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact tagcaattag
     1201 accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta aaagtaatat
     1261 gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt cttcaactac
     1321 attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat cccaaaaacg
     1381 ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca aatcaatgtc
     1441 taaagggttt ttagctaata taattgcttg aataattgga attggtttgc tgattcctga
     1501 agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta gttcaacaat
     1561 caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa gtttttctcc
     1621 agttctttta aaacctttct tgtcgactaa taccccatca acatttactt cccctatgtc
     1681 atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat ttagaagtat
     1741 cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt catagggaac
     1801 ctctttctat accttgaatt tacactttaa gtgcaacaca taaaaatgag tcataatcga
     1861 tatccaacta gtataagttt gtcgagaaca ctcctagaag gaaaataatt atgagccagt
     1921 attgtcatct tactatactc aaacgagaaa gaatctttct gcttcacgaa gaaggatttt
     1981 ctattagtat aattggcaac gacattcagc atagcccgtt tactatttta catgaactga
     2041 gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa aaaaattatg cttatcgaag
     2101 aaaaaaatgc ggacgttatc acattctaaa tgatccgtga gtttggtcaa ttgttcgtca
     2161 tttatttgtc gattttcagt ggtcgccaga gaaaatctcg aatcgtttga agcatgaaca
     2221 tatggacttt tcaattagtt ttatcacgat ctaccgtgca atttgcaatg atcttttcga
     2281 tacagaaccc ctctcacacg gcaataaagg cctgatccgt tcattgcacc atcgaggaaa
     2341 aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag atttgcctta ctcattcgat
     2401 ccatggccat ccaaaatccg cagatgatag aagtgatttt ggtcattgga aagccgatat
     2461 tgtagctggc aaaactagtg gtacttgttt agtgactttg acagacagaa aatcatgctt
     2521 ccttttagca ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg agaaaatgat
     2581 tgaactttta agcagtattc caaatgaggc ggtcaaaacg atcactccag atagaggaaa
     2641 agaattctcc aatcatttcg cagttacaga agtattgaat aatatgccct tttattttcc
     2701 agatcctcat gcccttggca acaatgaaca aattaaaata cgaatagact cattcgagaa
     2761 tacttgccaa aatcgaaaga gatagctacg gtagataact caacgataaa tcagatgatt
     2821 gaaaaactga ataacagacc tagaaagtgt ttagattgga gaacacctta tgaagtcttc
     2881 tttgataaag tgttgcgctt gatttgacaa ttcaagaaat aataaaaagc agatcaaatc
     2941 caaataaggg aaaaatgatc tactctggta ttatcaatta attggccaag ctattatgcc
     3001 gttttgctaa acgtctactt cttccagtat tgaccttgat aaaacaacaa tcaggatccc
     3061 gttttaatac tctatgaact gtgtcaatac taacatgtaa agttgcagca atatcagcta
     3121 atgtactccc atttaatctc atttgctttg caaattgata acgccgataa gcatcaatat
     3181 tcttggaatc catagacaat aatacttttc ttaccatgta tttcctccaa agattggatt
     3241 ttaatgggcc atcatttctt gagcaatctc atgctcagat aatgaagctg gatagaaagt
     3301 aggccaatta tgcatttcat tataaagttt ttcttgactt tctccaccaa agaaaatatg
     3361 gaaatgctct gccttacttg gtgcaattcc atgatcgcta aattgaatat atttaaaacg
     3421 accagcacct gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc ctttttcata
     3481 attcaaaatt ttgtatccaa catatttgta tgtgtattga tgatgttcac cgttgactac
     3541 aaaatcaatg gtgttgtctt taatgttgat agtatcaata tctgtcttat agccagtagt
     3601 ataataatct ttgtactcgg cagctgtcat gtctttattg agttttgctt tgtaatcgaa
     3661 aacttcatcc aaaattccat cttttaacaa cggataaaca gattgccatt cgcctgtata
     3721 atcactcaat tcgcgatcct tcacatcggc atctttaaag tacccattag caactgtctt
     3781 ttcatccttc gcttcttttt ctggaactac ctcttcacca gcaaccatag ttgttttctc
     3841 taacgctttt agattgtctt ccattactga aacataatct tcaccattat ccatttgttc
     3901 ttttgtaaga ctttcaagag gattcaatac ttccaatttt atcccagctt cattggccaa
     3961 agtttttgcg attttatcat tagcattctt ttcaaagtaa atataattga tcttattttt
     4021 tttaacatac tcttttaatt cagctaatcg accagaagat ggctcttctt caggacttag
     4081 tccagcaata ggaacttgta tcaatccata atccaaagct aaatatccaa aggctgcatg
     4141 ttgtgtcaca aaactttttt gtttcgcttc tttaagacta gtggtatagt cagcatccaa
     4201 ccgtttcagt ttagtcaaat acttttcagc atttgtctca aatacttttg cttttttcgg
     4261 atacaattta actaactgat ccttgatatt tgaaacttcc ttaattgcca ttttgggtga
     4321 aacccacgta tgaggatcta gttcatgatg gtgatcttct tctccgtgat catgatcttc
     4381 ttcttcacta cctggcaata aaatcatgtc ttttgttcct tctactacat taggcccagc
     4441 tttcttccaa ctttctttag cttttggtac ccatgattcc atgttttcgt tatgatatac
     4501 aaaaacatca gcatcatgag cttttgccat atccttagct gagggctcat agtcatgagg
     4561 ttctgatccc gctggaacca ttaaatccac gttcgcttca tcgccgacga tgtttttggt
     4621 gaaatcatac attggataga atgttgttac aatttctaat tttccatctt tatcattcga
     4681 tacttttgca tcattagtgc tggagcaagc tcccaataat cctagagctg ctatcccaat
     4741 aagtcctaca ctgattttct tcattctttt tccttcctta attatgctaa tttcttcatt
     4801 ttattttgtt tatgttcttt gataattttt aatgcccttt ttctagtttt gatattcggg
     4861 cttttaagtc gtcgatatgc actagatata tccgttttct ccattagaaa ttacccctaa
     4921 tcaatactta tcattatttg ctttccgcca gtaatttatc aaaactctgt ttgatccttt
     4981 cttcatccaa catttttcca attagaataa tagtggtttg gtctttttgt cgagtggtgt
     5041 attgaaaatt aacctgttcg tttacccctt gtatggcaac tataaaatct tgatcttgca
     5101 aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc agccggtcaa
     5161 ttaattggaa taaaaaatct ttttttatgt cttgatctga agttaagaga attgatttaa
     5221 actcatgttc ttgatgatgt tgatgatagt gtttgccatt atagtgattt tctgatgaat
     5281 cactttcaaa aactaaggga tgattgaatt tttccaaatt ataaaaatcc tttttttgaa
     5341 tcgtttcact atgtgtgaac attcgaatat cggataaagg gttaatactt ttgatttctc
     5401 tctgtaattt ttttagtgca gataaattca agttttgttt tctagacaca aaaatgcgat
     5461 ctgctatgac aagttgctta agggcttgtg gttctctttc aataagttca aaactgtcac
     5521 tatcaataac agtcaataaa ctatcgatat atactcttcc tcttagttga ggcgctgtta
     5581 aaagtgtttg aatgattggt tggggatctg ctatacctgt agtttcaatg atgatttgtg
     5641 aaattggttg ttgatgttct tctattgttt ccagtaaaga aatcaatatt gctaataaat
     5701 cactgcgaag actgcaacac atacatccat tatttagttg taacacccgt tcctcgctat
     5761 gaactaataa tttgtgatca atcccaactt caccaaactc attttcaatt ataataattt
     5821 cttccctggg gtagggtgag acttgcaagg cttgattgat tagcgttgtt tttccagccc
     5881 ctaaaaaccc agaaacgatt gtaactggta tgcccatttt gtcacctttt taccacttag
     5941 ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt ggcgaatact tttttaattc
     6001 taaccgttca gggttattcc gtttattttt gctggtgaga tacagacgtt ctccagtttc
     6061 gacacattcc aaaataatat tttgacgcat aaatcttcct ccttcaatac ttatcgatct
     6121 tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta actcgccaga ttccaattgt
     6181 gaaactaaat tttctttcat atcaacaggt atctcccaat aaccatcaat acattgaaat
     6241 aactcctcgt catactcttc aaaaattgga tagattctta aaaaatcatc aaatgataag
     6301 ccttggtcct catagtaatc tttgactaat ccattttcaa ttgcctgttg ggtcggtaaa
     6361 cccgtttcag taataattcc tctctctacc atcgaacgat aaaaagtgca tttatctagc
     6421 gattgctggg tcgtttcaag ataatacttt aatccttctt caactaaagg tgggtaaata
     6481 atttccattt tgattccctc ctaaaagtta tatattaaac tattttgaag ctcctttaat
     6541 tactttcttt ccattatagt acccttttaa agatacatga tggctacgtc tataatcacc
     6601 attcgcttcg tcaaatgaaa tttctggttt tactacagtt tgatgagttc ttcttaaccg
     6661 tttcttcgcc ttagatgttt ttcttgctgg tactgccatg atatttcctc cttttaccaa
     6721 cttgcttttt ttactccagg aatcaaccct tgatgggcaa gttctctaaa cttaatacgg
     6781 gacataccga actttcgcat ataccctctt ggacgaccat ccgtctgatc acgaagcctc
     6841 agacgattcg gatttgaatc tcttggcaat ttagcgagag cggtgcgatc tcctgcggct
     6901 tttaattcct gacgtttatc tgcgtacttt tcaatcatcg ccatttgttt ctttgcttta
     6961 gcaattttcg acttttttgc catatacctt tatactcctt ttattttgat ttagacatct
     7021 caaatcgtaa tcgttacgtt ttgacactga tactttacca acaagagttg ctcttgtcaa
     7081 caataaataa catgaaataa aactcatatg aatagataac tatttgaaaa cgaatttatc
     7141 ggtacataga gataatcaaa tcatttaaaa gaatatgcag tttaagctct gaaaaataag
     7201 aattcacttt tattagtcca atcaaacaca aataccgttt taagtagctt ttcctctact
     7261 taaaacggca atatgaaaga aactataact ttttattcaa tcttttatgt ttaactggtt
     7321 tatcgccaat tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga
     7381 actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt
     7441 attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta
     7501 tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc
     7561 tttatgagat ttttgtatat ttctgacctt cgatacaact cactatgagt accttgatca
     7621 actatttttc cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga
     7681 tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt
     7741 ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt
     7801 ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg
     7861 ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta
     7921 gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata
     7981 ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta
     8041 aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat acagccttca
     8101 tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga accactagca
     8161 cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg
     8221 gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga
     8281 ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga
     8341 gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt
     8401 attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag
     8461 tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc
     8521 atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt
     8581 ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat
     8641 tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg
     8701 gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa
     8761 ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa
     8821 aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca
     8881 acgtcagaca tgatagttcg agataaatct gataaattat gttttgaaaa ataggataat
     8941 ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat gcgcactttc tttataagta
     9001 ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaacg
     9061 aacgatactg taaaataaag ccaaggcggc tttacatttt ctaaaactaa ttcgtctatc
     9121 agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa gctagaaata
     9181 gatgctttaa ccaaatcttc tgcgccctga ttagttaaag cgaatttgtt tttataaaaa
     9241 cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata
     9301 cacttgatgt ttagccatta attcattatg atttccacgt tctactattt tcccttctga
     9361 caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta tcataataac
     9421 tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc
     9481 aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc
     9541 aatagctata cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt
     9601 atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc
     9661 aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg
     9721 attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga
     9781 atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc
     9841 tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat
     9901 tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa
     9961 gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag
    10021 ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc
    10081 aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa
    10141 aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa
    10201 aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac
    10261 aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc
    10321 tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc
    10381 tcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat
    10441 aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat
    10501 tagaaccaat ccaattttaa aattaatgaa aaagcctaag gcaactgata aaacaggtgt
    10561 tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc
    10621 gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact
    10681 tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag
    10741 gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac
    10801 agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta gagacttaag
    10861 aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa
    10921 agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac
    10981 tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc
    11041 tttccgtaat agcgtaaatt agagatgtac aaacagatag aaggtaaact acgttctatt
    11101 ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta
    11161 caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg
    11221 ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata
    11281 taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag
    11341 gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc gccatactaa
    11401 tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa
    11461 caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata
    11521 tgattccaga taataatcca aaaatccctg gggatatgct gtgaccaaac gccccaccaa
    11581 tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa
    11641 taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt
    11701 aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt tttttattcc
    11761 accatttttt aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt
    11821 ggctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat
    11881 attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa
    11941 tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg
    12001 atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat ctttactaac
    12061 tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat
    12121 ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt
    12181 ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt
    12241 ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt
    12301 ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac
    12361 ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa
    12421 caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa
    12481 ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa
    12541 atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc
    12601 atccaatata attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg
    12661 ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc
    12721 agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga
    12781 aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac
    12841 gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa
    12901 taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt
    12961 taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac tacctttttt
    13021 tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac
    13081 attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa
    13141 cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt
    13201 tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag
    13261 ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag
    13321 acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag
    13381 tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt
    13441 tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac catgagatca
    13501 ttacgaccgg caacattcga aatacaatat ataaggatgt cccaaaaaat atagtaaaaa
    13561 actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga
    13621 tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga
    13681 aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat
    13741 ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga
    13801 tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta
    13861 tcaaaaagca taactgagaa caattctcaa tgcaagcttt tttttaatta actcagtaat
    13921 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
    13981 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
    14041 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
    14101 tctagacaac agactgtctt ttttgtcaca aatgatgtaa ttttttcaac aatatcttga
    14161 tatttggttc cagctgattg gaataaaacc aaatcgaata tacgtttatt ctcaaaaata
    14221 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
    14281 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
    14341 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
    14401 gcacccgtag ttacgcctgc ggtcaagcac agttgcctta tcttagtttg ctgaaatcct
    14461 actttttcaa aatggcttag tccactttct attaattttt ggtgagttgt aatataatct
    14521 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctatattatc ttacacagga
    14581 cttgaagttt caatcgttaa cgtctattac tgaactcatt ccttagagct aaactaatga
    14641 gaaagagcaa aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
    14701 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
    14761 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagcc
    14821 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
    14881 aatcctatat ctaaatagtc ctcatatcta agtttagtca gcatctcact tacagatgct
    14941 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
    15001 aattcctgaa ttgctagtaa ataaattagt atagtagttg ttttaatttg taccatacct
    15061 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
    15121 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
    15181 agataggaat attggtatct tttgatactg ttttcattgg tctatcgtcc acactgcttt
    15241 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
    15301 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
    15361 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
    15421 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
    15481 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
    15541 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgggga tcttccttcc
    15601 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
    15661 ctttgttttc ctctttgttc gcatttttta ctaattttgt aaaccaagca tttcctccag
    15721 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
    15781 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
    15841 ttttatcttt tgctatattt tcagtaatat ctgctaaaat cgaattagta actactacct
    15901 ttaatttttc gttcgaagcc actgtatctt tgtctttact atttgttgaa catgccccga
    15961 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
    16021 taactttctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
    16081 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
    16141 aatgaacaaa cctagcaatg atgagcacgc tccgactgtc gccgaaataa atatcatagt
    16201 ttttaaactg tttgcatata aatatgctgt agcagctggt gtaattaaca ttgcaacaat
    16261 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaatcattaa
    16321 tagataatga taaaaactga cattcatccc cattgctttt gccattaatg gatcaaaaga
    16381 agtgattaat aattctttaa aaaatagaac aactgtaacc aacactaaaa cagaaacgcc
    16441 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata agatatgaaa
    16501 taaatccgtt gagctatttg caactcctat aaggataact cctaatgcta aaaaagaact
    16561 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
    16621 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
    16681 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
    16741 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
    16801 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
    16861 attattcccc ttgtatatta gagttagggt tttaaccttc cggttttgat tggataaccc
    16921 tatgaaccat cgttatttta atttggtatt aactcatttc ttttgagata atatatttat
    16981 tggattgaga ggctaagccg ttcccccctc tccaataaat atagtatctc cataggcttt
    17041 ttttaaattc tcttcattaa atacagattc tacacttccg tgagcaatta gatttcgatt
    17101 caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat gcacaattag
    17161 cactgttttt ccctgatgtt tcaattcttt taacgtatcc ataattattc gttcactaac
    17221 taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt gagcaagaca
    17281 tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt gatgattaga
    17341 atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt ctgcattttt
    17401 aattctttga aaaactttca ttccggcata tgttcccata gatacacatt cttttacttt
    17461 tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct tcaccagttt
    17521 tttattgata tcttcattat taatggagac ctctccctga tgagggataa tatttaacat
    17581 tgctttaagc aatgtagatt ttccagcacc atttggacca ataatacctg ttaaagcacc
    17641 ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac aaacattaag
    17701 ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca cgatcatatg
    17761 gtatacaaaa attatatttt agtcaaccct aaattttata atttagattt ttttctgttg
    17821 ttgaagatta ggatgtcaaa attatttgaa actactcgta tagtaaatag taagtaattt
    17881 tcaaaacacg aagaaaaaca aaaagaaaaa aggaaacgcc gaagcatttc ctttcagtga
    17941 tttaatgaga acaacacgat ttgacttaga gtctcaaaaa gtcgaatagg tacactcatt
    18001 taagtgtctc cattcgactt tttggggatg agctggctat tgaggtccgc ttaccctatt
    18061 gaataaatac aagattgggt atttctaata agtccgccct tcatattggg gtgatcgggg
    18121 cgaacatgcc atcttttcat ataccacctc ctcgacactc aataatcaaa gattctcgtt
    18181 aatcttcttc gatgcttgca atgatgtttt caatattcat ataacgaaaa tcttcttgtt
    18241 tgatataaac gatctgagcg gtacattttt cctttttgat gatttctttt actttttcct
    18301 cagaacgtgc gacctgcggc gttaaaagta aaacgtcata gtctttataa ttcagttttt
    18361 cttcaacgtt tcggatcgtt gcataatcta tctcatacga tagatttttg gctctgatcg
    18421 tttcaagcat ttttgatacg agcagcgacg cactgattcc ggtctcgcaa ataatcaata
    18481 gatccttatg cataataccc tcctgttttt agcctgcaaa gccaattggt gaaaatgcca
    18541 cataagttaa aataacaatg actaccaatg ctatagcagt caccttggtg tatttccaag
    18601 gctcgatatc gacctttgta tcgaatgagt tcaacacaaa ttctccatca ggtttcaatt
    18661 tggaaaccac catcaaaatg ataatgtcca gaacgaataa tgccgtcaaa acataaagga
    18721 aatggatatt ttggaaaagc atttttgata gtgcatacaa gacgatatgc acggcaatcg
    18781 tcgtttttgc cccaaaagca gttgcttttt ttgaataaaa ggcaaagaga atgatcacga
    18841 gcagcggcat gttgtatagt ccattgaatt cttgaacgaa ctgatacaat ccagccggtg
    18901 caaaagaaat gaatggcgct acgataatgg agataatccc cacaacgacc gttactgttt
    18961 taccagcacg agcgatttgc ttatcatttg ctttcttatt gataagcggt ttataaaaat
    19021 ccagtgaaaa caaggtcgat gtcgcgttca aagctcccgc aaatgatgat aagatcgcgc
    19081 caaagataac agcggcgaaa ataccaaaga tcattttagg caatacttca ctgactaaaa
    19141 aaggatacgc gttatctgga tttgacatta aactatcgcc aaaaatattg cgagcgacga
    19201 tccctggaaa gacaaggaat aacgcaccta agattttgaa gacgccgact aggaaagcgc
    19261 ccttttgtgc ctcagctaaa ttcttgccgg acaatgcttt ttggacgatc atttgattgg
    19321 tacaccagaa atacagattg ttaaacagca tccctgtaaa tagtgtcggc catggaacaa
    19381 cagccgagtc gatcgatccc acagagttaa gcagccatgg cgtgtttacc acaacgtgtt
    19441 cgatcccgcc aacaaagctg ccgtcaccaa gcttcattaa gcctaaaatt gggaccagca
    19501 gaccacaagc cagcagaccg actccataaa tggagtcact attggcactt aacgacaagc
    19561 cgccgatcag cagatagagg atgccgacaa taccaattgc catcgctaca attatgatcg
    19621 cgacgatcgg ctggacgccc agcagctcat caatatggaa gatcttattg aacaccagcg
    19681 atccagaata aagaaccacg ggtaagaaag aagtcatata ggtcacaata aataagatcg
    19741 aaataattcg tttggtaacc gtatcataac gaatctcgat gaaatctgag attgtattga
    19801 tgccgtattt aaaatatttt ggcaagaata tcacagccaa agctacgatc gcaatcgtag
    19861 cagttacttc ccaggccatt acttccatcc ctgcatgata actttgacca ttttgtccga
    19921 ctatttgttc ggtggacagg ttcgtcatga tgatcgtgcc ggcgattggc aaagcagtta
    19981 agctccgccc tcccatgaag aacccttctg atccagaagt atcgacactt ctggatctag
    20041 aataggcaaa taaccataca cagatgacaa tcactaggaa actgattata gaaaaaagat
    20101 tcatcgtcta gctccttttc aatagattaa agaccggtga cctctcgaat gtagcttctg
    20161 gctttgactg catattcaaa tggattggct ttagccgggt cctgttctgc ttcaactacg
    20221 atccagcctt catagtcgct ggcatggatt gctgtccaga ttggagtgaa atcaatcatc
    20281 ccatcaccag gaaccgtgaa gacgcctttc ttcacgccgt ttaagaagct gtctttttcc
    20341 ttttctactt cttcagccgt ttcttgacga atatctttga aatggatgtg tttgatacga
    20401 tcttgatgac ggttgtaaac cgcgattgga ttttcaccag aaaagaccaa atgtcccgta
    20461 tcgaacaata atttaacttt gtctggatca gtcatttcca ttaaacgatc gatttcttcc
    20521 gtcgtttgaa cgccggtacc catgtgatga tggtaaacga tctccatatc cttttcgtgg
    20581 gccaaatccc ctaatttttc taatccttcc gccagttttt tccactcttc atcagtgaaa
    20641 actggtttat catcaaagac tggcgctgtc ataatccctt ggatactatg accctgctca
    20701 gctactacga tgactttagc gcccatcgca tgtaagaaat cccgatgtgc tttgaaggct
    20761 tcggcagttt cttcaaaagg tttagttgtc agaaacgcac tgaaccatgc actggcaacc
    20821 tttaagccgc gaatattcaa atattgttcc aacacctctg gatcttttgg atatttgttg
    20881 ccaatctctg ttccggtgaa cccagcaagg gccatttcac tgatacattg ttcaaaggta
    20941 ttttcactac caagctcggg catatcatca ttggtccacg cgattggtgc gatggctaat
    21001 tgaatttgtt tggtcatatt ctcacactcc tgcttatttt tttagttgta ctaaactctt
    21061 cgtatcgaat gattcttgac aagccaacgc gatcttagtt gattgcaatc catcttctgc
    21121 attaaccgct ggttgacgtt tttcttgaat acatgagaca aattcttggg cttcacgaat
    21181 gaaggcttct ctgaatcgtt ctgggaagtt ttgtgaacat ggacggatga tcccttgatc
    21241 gttcatgatc gtcacaagat ttttctcagg cgcttgcgct acccgcagca ttcctttggt
    21301 tcccatgatc tctgtttcga catgatagcc atgggctgcg tttctgccgg caactagtaa
    21361 agctattgtt ttatcgctca ggctcatcat tgcggctcca gtttcaagct caccagcctg
    21421 atccaattct ggatacgccg catttttccc aatcgcccac acttgatcga cttcttggcc
    21481 ggtaaaccag cgaaccaaat caatatcatg gatcgccata tctaagaaca agccgccgct
    21541 gtcgttattc atggcaaatt tgacaaagct ttccaagcct tcgctcggat cgatcccata
    21601 acaacgaatc agtgtgattt cgccgatttc gccatcttct accattttct tcgcatactg
    21661 atacgactca tcatagcggc gcatgaagcc taacatgaag acctgttcag gatgctcttc
    21721 gatgactttt tgggtctttt taatatcttc aacttcaagg cctaatggtt tttcagtaaa
    21781 gacatgcttg ccattttcca tcgccaagcg gacttggtca cagtgaaagc ctgatggtga
    21841 aacaataaag actgcatcga tcacggaact tttgaccatt tcttcatagc tggaaaaagt
    21901 atgcttgacg cctaatttct tctgtgcgaa tgctaattct gtttcaacta aactgcacgc
    21961 agccgtaagc tcacaacaac tgacacctgt tgctaaattt tgtgcatgta cttttcctaa
    22021 tcttcctaaa ccgacgatgc ctactctaat gttgttcatg aaatacctcc gttttctgag
    22081 aattactcat tgtatagtgc tggtcgttca tccatttgaa tcattacttt tgtttttgtt
    22141 tcacgagact catgacaagc catcatggtg atcgctgcta catacccatc ccaagcggta
    22201 ggaccgacaa ccgcatcatt tttgatcgaa tcgatccaca gcttgaattc ataatcatag
    22261 gcatccaaaa agcgggtctg ccaatctggt gaaacaggtg tgtaattttt ttcttccgat
    22321 ttaattcgtg tataggctgg gtccgtcatt cctacttctg ccgtttcgaa aaccacatca
    22381 catttaatat cgtagccaaa gcggcaattc acaaagattt ccaaatcaat cgttacaccg
    22441 gattttgttt gaaagatcag cagctgcgga tcatgcaaat cggcatggga gttgctggtt
    22501 tttttcggca agatcatttg aacagattca taatcttcat ttagcaacca gcgaagcaca
    22561 tcaatctcat gaatcgcggt gttgctgatc gccatcggtg ttgtgtagtt ttcatctgcg
    22621 ctaggatttc tatgggcaca atgcatcatt aatgcttctc cgtattgctt cgattcgatt
    22681 gcttctttca gttcaatgta gccgcggtca tagcggcgca tgaagccaac ctgaaccaat
    22741 ttcttcccat aagcaacttc cgcatcaacg atccggcggc agcctgctgc atccatcgct
    22801 aatggctttt cgcagaaaac atatttccca tgcttgattg cttctaacac atactgctca
    22861 tgggtcgggt cccaagaagt cacaacaacg acatccacat cttcagaagc gatcaatgct
    22921 tctccggttt caaagaattt agcatcgaca cgtttggcaa cctctttagc agcttttaca
    22981 ttgatatctg agactgctgt cacataagcc ccttgtgttt tctgattgat cctgtcgata
    23041 tgctcgatgc cgattgcacc tgttccgata acgcctactt ttactctcaa ttacttatcc
    23101 ccctgttctg ttgtttaagc acacatagta tgcgcttaca aaattattcc tgtgtagctt
    23161 ttattcttaa aaggtagagc cctctctttg ctccgccttt tactacccat aagcttgtac
    23221 gtataattcc ttaatttgac tatgactagg caccttttta ttattttgcg gacttccgct
    23281 ggtcaacgca tccgcggcca ttttgtctaa ggctgccata aacgcttgct gatcaatacc
    23341 ataatctcga atcgttggga tcgacagctg ctcacagagc tcttggattt ttttaatcag
    23401 ataatccgca gcctcttcca gcgataaatc ttcggctgcc ggataaataa atctggcgat
    23461 cgtcgctaac tcagtctgaa tagcatcctt tgagaattca aaaaagaccg gcaataacat
    23521 cgcatttgat atcccatgag gaatgtgaaa aagtgccccc accggtcgac tcattccatg
    23581 aatcaatgtg actgacgcat tcgaaaaggc aatccccgcc tccgttgaag ctaagctcat
    23641 ttgttctctg gcttcagtgt cgtcgctatt ttcatatgcc tgagaaagcg tgttcaaaat
    23701 tttcttcatc gcttccagtg aaaatagttg cgttacttca ttggctttga ctgaaacata
    23761 agattccaaa gcgtgacata gcgcatcgac tcccgttgcc gcggttactt ttggtggtac
    23821 agttttcgta aaatcagagt caacgattga gatctgtggc atgaaaacga gatccttcat
    23881 catcattttg actgctgttt ttgtattggt aataactgcc acatcggtta cttccgaacc
    23941 agttccagca gttgttgaca ctgcaatcag cggaatgccc gcttgcagct gatcaaaatt
    24001 tcttgcatga atcgcttcga gacttaattg attagcagcc aaaacggcta ccaccttggc
    24061 tgtatccaga cagctgccgc caccaaaagc aatgatatga tcacattgat gcttctgaaa
    24121 aagcttcagc gcatcataaa cattttcttg cgtcggttcg gcttgaatgt ctgtatactc
    24181 cgctaattga tcatctgttt ttcccaacag ctccttgatc ttttgaagat gcccggtctg
    24241 ttccataacc gtatcactaa tgattaacgg cttgttgcct agttgttctg attcttccat
    24301 aagaaattgg attgttccgc cgccaattct taaattggca ggcattttca gttgaatatt
    24361 cacagaaaaa acctctctct ggcttatagc ctataacaga atcctcgaag ctctttaaca
    24421 gagcttcgaa gcaaatcttt aaatctcact ttgcgcataa cgtgctgtaa caactttccg
    24481 atgggtataa aactcaacac tgtctttgcc attggcatgc aaatcaccaa agaaagagtg
    24541 cttccagcct gaaaaggcaa accaagccat cggtgcaggc accccaagat tgacccccag
    24601 catcccagca tcaacatttt ctctgaagta gcgaatcgct gatgcgttgt tggtatacag
    24661 acacgaacca ttcgcaaatt cagattcatt cgtcagctca acggctgcca tcaaatcttt
    24721 tacccgcacg attgatgcga ccggggcaaa aatctcatcc tgccagatct tcatatccat
    24781 cgtgacatta tcaaaaatcg ttggtccaac aaaatagcct ttttctggaa tgttttcgcg
    24841 accatctagt accagtttcg ctccttcatc gacccctttt tggatatagt caaaggtccg
    24901 ctgctgtgca tcttttcgaa tcactggtcc taaaaagacc ccttcgtcca agccattgcc
    24961 gatcttgatc gcttttgccg cagccttaaa tttttcaaca aactgatccg cgatttcctc
    25021 ttccaaaaca atgacggagg ctgccatgca gcgttcacca gcagaaccaa aagctccgcc
    25081 aataaggtcc ttgactgccc gatcaatatt ggcatctttc agcacaacaa catgattttt
    25141 cgctccagtc aacgcttgta cccgttttag attttttgtt ccttcacggt aaacgtattc
    25201 accgacattt tttgatccta caaagctgat gccttttacc agtggatctt gcagcaatgt
    25261 gttgacaaca tcgtgggcac catagacaac attaaaaaca ccgctaggaa agcctgcttt
    25321 ttcaaccagc tcaacaattt tctccatcaa caatggtgtt ttttctgaag gcttcagcac
    25381 caccgtattt cctgccgcca gagccatcgg gaagatccag aagggcacca tcattggaaa
    25441 attaaacggt gtgatccctc cgattactcc gattgggtaa cgataattcg tgatctcaat
    25501 atcagtcgcc gctgttgata aagaatctcc catcattaaa ttggtgattc ccgctgcgtg
    25561 ctccacattt tcgattcctc gttgaacttc tcccagtgac tcggacaatg atttcccatt
    25621 ttccagcgtg atcaaacgcg ccaattcact tttatgatct gttaaaagct gatgcagctt
    25681 gaacagataa cgtccgcgaa caggaacagg aacttttttc caagtttgaa acgcagcatc
    25741 ggcagcttta actgcttcgg ctgtctctgc tttgctagac aacggtacat tcgccagcgc
    25801 ttcttctgta gccggattca ttacttcttc aaactctgtt gagcgactag cgacccaacg
    25861 accatcaata taatttctta aaaatcttgt catttatttt cctcctcgta aaatatcatt
    25921 tgtctaatag tttttattca aaatgtttct agattataaa atttattgaa taacacattc
    25981 ctaacggaat ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DADXTH010000039        25891 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.039, whole genome shotgun sequence.
ACCESSION   DADXTH010000039 DADXTH010000000
VERSION     DADXTH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.039"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            620..1483
                     /locus_tag="IWI97_001536"
     CDS             620..1483
                     /locus_tag="IWI97_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBC2463036.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1612..3234)
                     /locus_tag="IWI97_001537"
     CDS             complement(1612..3234)
                     /locus_tag="IWI97_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2463037.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3483..4004
                     /locus_tag="IWI97_001538"
     CDS             3483..4004
                     /locus_tag="IWI97_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBC2463038.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4190..5920)
                     /gene="spxB"
                     /locus_tag="IWI97_001539"
     CDS             complement(4190..5920)
                     /gene="spxB"
                     /locus_tag="IWI97_001539"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBC2463039.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6085..6903
                     /locus_tag="IWI97_001540"
     CDS             6085..6903
                     /locus_tag="IWI97_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBC2463040.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7006..7203)
                     /locus_tag="IWI97_001541"
     CDS             complement(7006..7203)
                     /locus_tag="IWI97_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463041.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7372..7950
                     /locus_tag="IWI97_001542"
     CDS             7372..7950
                     /locus_tag="IWI97_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBC2463042.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            8045..8182
                     /locus_tag="IWI97_001543"
     CDS             8045..8182
                     /locus_tag="IWI97_001543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463043.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8995..9498)
                     /locus_tag="IWI97_001544"
     CDS             complement(8995..9498)
                     /locus_tag="IWI97_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBC2463044.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9857..10882
                     /locus_tag="IWI97_001545"
     CDS             9857..10882
                     /locus_tag="IWI97_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBC2463045.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11205..12083
                     /locus_tag="IWI97_001546"
     CDS             11205..12083
                     /locus_tag="IWI97_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBC2463046.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12559..12675
                     /locus_tag="IWI97_001547"
     CDS             12559..12675
                     /locus_tag="IWI97_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBC2463047.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13603..13941
                     /locus_tag="IWI97_001548"
     CDS             13603..13941
                     /locus_tag="IWI97_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463048.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            14035..14265
                     /locus_tag="IWI97_001549"
     CDS             14035..14265
                     /locus_tag="IWI97_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463049.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14665..15036
                     /locus_tag="IWI97_001550"
     CDS             14665..15036
                     /locus_tag="IWI97_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBC2463050.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15310..16002)
                     /locus_tag="IWI97_001551"
     CDS             complement(15310..16002)
                     /locus_tag="IWI97_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBC2463051.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(16005..16790)
                     /locus_tag="IWI97_001552"
     CDS             complement(16005..16790)
                     /locus_tag="IWI97_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBC2463052.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            17027..18427
                     /gene="lacG"
                     /locus_tag="IWI97_001553"
     CDS             17027..18427
                     /gene="lacG"
                     /locus_tag="IWI97_001553"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBC2463053.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18452..18769
                     /locus_tag="IWI97_001554"
     CDS             18452..18769
                     /locus_tag="IWI97_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBC2463054.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18782..20455
                     /locus_tag="IWI97_001555"
     CDS             18782..20455
                     /locus_tag="IWI97_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBC2463055.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20469..22100
                     /locus_tag="IWI97_001556"
     CDS             20469..22100
                     /locus_tag="IWI97_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBC2463056.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22591..24156)
                     /gene="guaA"
                     /locus_tag="IWI97_001557"
     CDS             complement(22591..24156)
                     /gene="guaA"
                     /locus_tag="IWI97_001557"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBC2463057.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24215..25138)
                     /locus_tag="IWI97_001558"
     CDS             complement(24215..25138)
                     /locus_tag="IWI97_001558"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBC2463058.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25334..>25891
                     /locus_tag="IWI97_001559"
     CDS             25334..>25891
                     /locus_tag="IWI97_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2463059.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEI"
BASE COUNT         7999 a         4514 c         5205 g         8173 t
ORIGIN      
        1 cgtgtcacag tccctttttc cgaacaaacg gtgttcaaaa gcttgcatct acggcactaa
       61 gcccaatcag atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg
      121 cagagcagct ttttcacaac ctctccacta aacggtgttc tcctgccaac tcctcggtca
      181 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg
      241 gagctaaacg gcaggctcac aacctcttgg taaacggtgt tcaatcagct gaccctcggt
      301 attagctcaa aaaacggcaa aatatgagga actatttttt ccgttttttt ctctaatact
      361 caggtcaaaa cgctgatttc acaacctctg attcacggtg ttccagaagt agcttctacg
      421 gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
      481 cttgaagctg accacttctg tcacaacctc ttattttgat tagaacttgc gaaaataaac
      541 cggttaaagt gtgtctagcg caagagattt gctataatag acgataaggg taaaatgtaa
      601 cgagaagggg ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg atctgccata
      661 ccatgtatta gcaaaagaaa aagttgattt tttatcaatg aatctccaat tgaatgatca
      721 agagttagta gatgacctcg gaaagacatt caattatgac cgctacatga atcagctgaa
      781 aaatggtgcg atgcctacga catcacaaat caatgttggc cgatatttag aatttttccg
      841 ttcttatgcg gaaaaacaga ttcctgtttt atatgtggct ttttccagtg gattaagcgg
      901 ctcttacagt agtgcgcttc aagcggttga attgctgaag gaagaatatg aagaccctga
      961 gatctatgtt tttgatacaa aagctgcaag tattggggaa gggcttttag tattggaagc
     1021 tgtaagattg aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa
     1081 ttatttgaaa gtccattcat gggtaacagt agacgatctg aaatatctag aacgaggcgg
     1141 taggatttcc aaagcaagtg ctactcttgg cagtctattg agtgtaaagc ctattattac
     1201 ggtggataaa gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat
     1261 ccagacaatc gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc aaatcgttta
     1321 tgtggcttac gctggggatc tagagagcgc ggagaaggca aaacggcttt tggaagagca
     1381 gataaagatg aaagatgtga agctttatcc cttaggacca acgatcgcca gtcacacagg
     1441 ctatggctgt atcgcgattt tttcgatggg aatcagtcga taaaagcagt aactcattct
     1501 gtaaaaaagc ccagaaattg attgtaacaa aaacagcgac tcttaaaaga tagatgagca
     1561 tcctatctct tagaggtcgc tgttttttta ttttttaagc taaataggcc attatgccca
     1621 taactcgttc aattgttttt gaatatctgc attttccaaa aattcatcat atgtcgtatc
     1681 agctcgatcg atcacacctt tatccgaaac ggcaatgatg cggtttgcca atgtttggat
     1741 aaattgatgg tcatgtgacg caaataagat tgagccagta aatgccatca agccgtcatt
     1801 caatgctgtg attgattcta agtctaaatg gttcgttgga tcatctaata caagtacgtt
     1861 agctttcgaa agcatcattt ttgaaagcat gcaacggact ttctctccac cagataaaac
     1921 attcactggt ttcaatactt cttcaccaga aaacaacatt cttcctaaga agcttcttaa
     1981 gaaggtgtta tcatcttctt ctttacttgc atattgacgc aaccagtcta aaatcgttag
     2041 atcagtatcg aattctttcg ttgtatcctt tggtaaataa gaattacttg ttgtcacacc
     2101 ccaacgtact gttccagtat ctggcgtaat ttcacccatg ataactttga atagcgttgt
     2161 tgttgttata tcattttctg cgacgaaagc gaccttgtct tctttgctca aattaaaaga
     2221 tatatcatct aaaattttct ttccatcaat cgttacactt acattttcta cctgcaataa
     2281 gtcgtttcca atctcacgtt caggtttgaa tccaacgaat ggataacgtc tagaagaagg
     2341 ttggatatcg tctaaagtaa ttttttcaag cattttttta cgtgaagttg cttgttttga
     2401 ttttgatgca ttcgcactaa agcgggcaat aaattcttgg agttctttga tttgttcttc
     2461 ttttttggca tttgcgtttg cttgaagttt agccgctagt tgactagatt ccaaccaaaa
     2521 atcataattt cctacataaa gtttgatttt accaaaatct agatccgcca tatgtgtaca
     2581 tactttgttc aaaaagtgac ggtcatggga tacaacgatc acagtatttt caaaattgat
     2641 caaaaattct tccagccaat tgatcgattg gatatccaga ccgtttgttg gttcgtctag
     2701 caaaagaaca tcaggtttac caaatagtgt ttgtgctaat aacactttta ccttttgtcc
     2761 accagttaat tcactcattt gctgatggtg caattcttca gggatgttca gaccttgcag
     2821 taaaacagct gcttctggtt cagcttccca tccattaagc tcagcaaatt ctccttctaa
     2881 ttcagctgct cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt
     2941 ttctttcagc acttcataca gacgcttgtg tcccatgatg actgtttcaa gtacagtata
     3001 gtcttcatag tcgtaatggt tctgtttcaa tacggccatc cgttcatctg gtcccatagt
     3061 aacggaacct gtggaaggct gcagatcccc tgataacact ttaaggaacg ttgatttacc
     3121 tgcaccattt gcaccaatca agccatagca gtttccgggg gtaaatttaa tattcacatc
     3181 atcaaatagt ttgcggtctg aaaagtgcaa actcacatca tttacagtaa tcaatttttt
     3241 tcctcacttt tatcattatt agttttgaca agtcttatag aattatatag gggaaaggta
     3301 aaagattcaa ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg aaaaaaataa
     3361 aaataaaagc ggagcgaaaa tagctactat aggatttatt agtttgcaac agcataattt
     3421 ttgacgttat ttataagagg gagtactata aaactgtaaa agaaatgaaa gaaggattga
     3481 aaatggatga aaaaagaacc atcgactggg gctcattgat tttgggaatt ttatttgtac
     3541 ttgtctcatt gttatcattt caagaccctg tcggaaattt agttgcaatt gtagtagtct
     3601 ttgcgatttt tgcctttatt aaagggatat ttgaactgtt tgtacggaac agattaaaag
     3661 agctaacagg atataaagga aagatgccct taataatcgg gatcatcgat attctagttg
     3721 gcgtcttttt cttattcaat attggtgcag gagtcgcagc attgcctttt gtttttgcag
     3781 tatggtttat tgctgattca gttttagcgt tatttactgc tgacttggca agaggagtaa
     3841 gtgaaggata ttattggttt acgatcatcg tcaatatttt aggaatttta ttaggaattt
     3901 tcttattgtt taatccaatt tcttctgcgt tgacattaag ctttttagtt ggattctatt
     3961 ttatgttatt tggtatcaca catatcgtct atgcatttag ataagtaaaa tcaaacaaat
     4021 aaaaaagccg gcttccgatt acagttttac tgttgtaatt ggaagccggc ttttttatca
     4081 tggacaaata tgcacgaggt gatttttagg tgtcgagaca aatttatgca aaaatgtgag
     4141 aagctatata cacaaatttt atcttaatac tcgaagcata gcgcagttgt cacaacctct
     4201 tttcatataa ttcattcaat actggcaaat catggacttc atatttttct ttgaatgctt
     4261 ggatttcttc ttcagaatat tttcttggat ctaaatgcag atcttctact ggcaatgggc
     4321 gcttgttatt gatcttcaca tcgattacta caggacgatc tgattttgca gctgcatcaa
     4381 aggcaaatcg aagttgcgag tattctgtga ctgtaaagct atttgccccg agcgcttctc
     4441 caactttacc gaaatctgca tcttctaaaa atacaccaaa tttcttttgt tccgtatctt
     4501 cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta ataattggaa
     4561 gctgatattt gacttgtgta atgatatctt gcatgaccat agcaaacccg ccatcaccac
     4621 ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc ggcaattcct cctggcacac
     4681 cattacccat agttgcgaac catccagatg tcgtatgttt ttgtttaccg ttcatattca
     4741 ataaacgaat agaatggata gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt
     4801 cagcaatacg attgatttct ttatagacag cctctggacg aatcggttct tcctctcgat
     4861 cttcaaaaga tttcaaccaa tttacccatt tttctttatt ttcttgattt gctaggtacc
     4921 agctatcagc agggcgagca tcccctaact cgacaagtct ttgcaaagct gtgtttgcat
     4981 caccgaggat cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga
     5041 tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga
     5101 caaataagat cagatcggct tctgccaatg cttcatttcc gggtttagta gcgacacgtc
     5161 cagcaaaacc aagaaagttt tcataatcat caggaataat tccttttgct agaacggaag
     5221 cgacaacagg aactgaaaaa tgttctgaga attctttgat ttgcggaaat ccgtttcgcg
     5281 ttccttgacc aacataaaga actggttttt ttgctttttc aagataaggc aaagcagcta
     5341 gcaaatcttt ttcttctggt aatatcacac ttgttttgtg agtgtgagcg gtggagaaag
     5401 gttgttcgtc aatttcttca aaaccaagat caacagggat agtcacgata gccacgcctt
     5461 tatgctcata ggcggcttta atggcttcat ctactacatg cggaagactt tgtggtgtca
     5521 tgactgtacg attgtatacg ctaacatctg cgaaaatagg attttcattt agttcctgga
     5581 aagagttata gttcatggat gtagaagcaa cttgtcctaa gaacgctact agaggcacat
     5641 ggtccatctg tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc
     5701 cgaagacagc gccgactttc cccgttaatt ttgcatctgc agcggctgcg agtgcgccga
     5761 cttcttcatg acgaacttga atgtagttga tttcggtttt ttcttgatat agcgcatcca
     5821 taattgaatt gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa
     5881 gaacttttac cattgcgatt cctgcattga tttttgacat ggtgtttcac tcctttagca
     5941 atgatgatac ataaattttt tgtatctaga gtatagaact ttttatcgaa aatcccaact
     6001 aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag tagtacatag
     6061 atagattcaa gagaggggaa tggcatggtt gaatggctat tgagacttga accttggcag
     6121 caagcacttg ctgggacagc ttttacttat tttatgacag ctcttggtgc cgggttggtt
     6181 tttttcttta aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt tgcttctggt
     6241 gtcatgattg ctgcaagttt ttggtctttg ttagatccgg ctattacaaa agcagaagaa
     6301 aatggagata ttgcttggct agttgtaagt attgggtttg gtttaggcgg tgtgttttta
     6361 tatatggcag ataaaacact gccccatatg cattttggac cacaacatga agcagaagga
     6421 ttgcctaccc atttaaaacg gacgatcttg cttgtttttt ctattacatt gcataacatt
     6481 cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag caactgcgga taatccgaca
     6541 gccgctatat tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa
     6601 ggagcagctg tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt
     6661 tacggacaag cttctggaat cgttgaacca attgctggag tgattggagc gctactagta
     6721 acgaaagtgg agctgctgct tccttatgca cttgcttttg ccgcaggagc aatgatttat
     6781 gtcgtggtgg aagaattgat ccctgaagca cagcagacat tgtccagcaa acgtcatttt
     6841 gcagtttttg gcgtcatgag tggatttatc attatgatga ttttggatgt ggcattagga
     6901 taaataaaaa aaatatatat gatatatcaa atagggccgc gaacttatat cgcgacccta
     6961 tttttttagt aagttatatc ataatgaagt gaatggtatc atacattaat aatagttttt
     7021 tctatgtgta tttcctttga tccagcgaac aagccagatg ataagtgcga ttgggattag
     7081 aactttgacc gcaaaccata aaagcccgct taggacactg acaaaaagac tgatgacgaa
     7141 gctagctgca attagcacta aaagaaccat gaagatattt aagaacatat ttttttgacg
     7201 catacaaaac cctcctcttg ttcattcttt actatagaaa aagtataaca gggattgttt
     7261 aaaaacacct ccaactgaag actgattttt acttcatcta cttaaatgat caaacaattg
     7321 ctttttctct ggtttatcat ctaaaataga gcggaaaagg aggagaatat catgcctaaa
     7381 aatcgtcttg aagcatttac agatgctgta atcgcgatta tcatgactat tttagtttta
     7441 gaacttcatg aacctaacgg agacacgtgg gaggcgctcg ctgcattagg ttaccgattt
     7501 tttatctata ttgtcagttt tatcatttta gcgatttatt ggaacaatca tcatcattta
     7561 tttcaagttg tacataagat aaatggacgg gtcttatggg cgaacagttt ctttgttttt
     7621 gcgctttcgc tttttccgtt tgcaacttca tggatcagtg agtacatcaa tagcctggct
     7681 ccagaaatga cttatggttt tgttaccttg accgctaata ttgcgtatta tatcttggca
     7741 aaagaacttg tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat gttgaataat
     7801 tacaagaaat cgtatctatc gattggtttg aatgtgttag gaattgtctt agggtattta
     7861 gtagcgccag tagccgtgct gatcgcaaac atcgtgattc tagctgcttg gatcatccca
     7921 agtcgtacca tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt taagagaaaa
     7981 atatagaaaa aataaataaa cgatcaaata tccgagtgat actagtattc aaagagggat
     8041 gcagatggat caaaaaagaa ttcatgtagg actgattact agttttgtca cgatgcttat
     8101 ttatttggat caaggtcatt tgacttggac agtagttggt gtcgtaggat ttttgacatt
     8161 attcacagca ataaaaaaat aaacatcttt atgtaatctg atgttctatc tatttaagaa
     8221 aagcatgttt acagcagtat gattctgttg taaacgtgtt tttatttgtg gaaaatttaa
     8281 tttttagaaa agtgtatcga aaaatagata gttagttttt ccacagtgga taacttcgta
     8341 agagataaaa ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata
     8401 tacaacagaa atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt
     8461 ggaataaaaa aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac
     8521 gacacagaaa ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc
     8581 ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag
     8641 ggagcctaaa taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct
     8701 ttttatagaa aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga
     8761 ttgagatatt gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct
     8821 ttttgttaaa taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa
     8881 taatatagta gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac
     8941 cgtcatagat acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg
     9001 aagacggaca gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta
     9061 tcccaaaata atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg
     9121 ataggcccat aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc
     9181 tacgccaata ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat
     9241 ccctaatttt ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg
     9301 aaaaaagaca atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa
     9361 agtagaaaaa gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt
     9421 aaatgatagc ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa
     9481 ttttgataca taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct
     9541 gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga
     9601 agcttttttt tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga
     9661 aaagttgcta tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc
     9721 tccaagttgt ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact
     9781 aggtaatgct gacaaacggg attggcattt catcttggtt cagctttttt tccaattact
     9841 aggaaggagg gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc
     9901 ttttcgtatc atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa
     9961 tcatgatttt ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca
    10021 aaacataccg atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt
    10081 tgctttcgtt ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc
    10141 tttggttgct ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg
    10201 cttgtttttt gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt
    10261 accgatcgtc tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat
    10321 tacaaaatta ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag
    10381 atttcacgaa gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct
    10441 ttccgtttat gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg
    10501 tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct
    10561 gattggatcg atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca
    10621 gttgcagacc atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag
    10681 aaattatgca gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat
    10741 tcgtggagca tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag
    10801 tttcatcggt ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt
    10861 tgttcgtcaa aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga
    10921 tttttgtcac aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa
    10981 ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa
    11041 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    11101 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11161 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11221 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11281 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11341 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11401 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11461 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11521 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11581 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11641 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11701 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11761 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11821 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11881 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11941 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    12001 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    12061 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12121 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12181 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12241 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12301 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12361 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12421 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12481 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12541 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12601 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12661 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12721 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12781 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12841 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12901 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12961 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    13021 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    13081 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13141 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13201 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13261 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13321 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
    13381 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
    13441 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
    13501 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
    13561 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
    13621 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
    13681 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
    13741 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
    13801 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
    13861 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
    13921 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
    13981 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
    14041 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
    14101 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
    14161 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
    14221 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
    14281 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
    14341 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
    14401 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
    14461 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
    14521 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
    14581 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
    14641 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
    14701 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
    14761 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
    14821 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
    14881 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
    14941 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
    15001 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
    15061 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
    15121 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
    15181 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
    15241 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
    15301 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
    15361 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
    15421 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
    15481 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
    15541 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
    15601 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
    15661 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
    15721 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
    15781 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
    15841 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
    15901 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
    15961 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
    16021 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
    16081 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
    16141 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
    16201 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
    16261 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
    16321 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
    16381 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
    16441 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
    16501 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
    16561 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
    16621 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
    16681 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
    16741 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
    16801 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
    16861 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
    16921 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
    16981 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
    17041 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
    17101 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
    17161 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
    17221 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
    17281 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
    17341 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
    17401 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
    17461 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
    17521 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
    17581 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
    17641 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
    17701 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
    17761 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
    17821 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
    17881 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
    17941 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
    18001 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
    18061 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
    18121 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
    18181 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
    18241 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
    18301 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
    18361 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
    18421 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    18481 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    18541 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    18601 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    18661 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    18721 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    18781 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    18841 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    18901 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    18961 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    19021 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    19081 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    19141 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    19201 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    19261 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    19321 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    19381 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    19441 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    19501 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    19561 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    19621 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    19681 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    19741 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    19801 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    19861 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    19921 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    19981 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    20041 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    20101 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    20161 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    20221 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    20281 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    20341 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    20401 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    20461 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    20521 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    20581 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    20641 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    20701 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    20761 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    20821 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    20881 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    20941 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    21001 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    21061 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    21121 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    21181 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    21241 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    21301 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    21361 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    21421 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    21481 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    21541 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    21601 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    21661 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    21721 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    21781 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    21841 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    21901 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    21961 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    22021 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    22081 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    22141 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    22201 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    22261 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    22321 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    22381 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    22441 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    22501 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    22561 gggattcccc ctccatatta tgttggctct ttattcccac tccactgttg ctggtggttt
    22621 agaagtgatg tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga
    22681 gattttttgc aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga
    22741 agttacagca cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac
    22801 agagcggatc cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc
    22861 tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt
    22921 gatttcgcca agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat
    22981 tgaatcaggc atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt
    23041 taatggttcg atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga
    23101 tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg
    23161 tgctaggaaa gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata
    23221 tacgaattca ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt
    23281 gtctaagaag cgatctcttg cattgacttt gatgatgttc aagccgaatt ttccgcctag
    23341 actttccatt acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga
    23401 tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc
    23461 acctgaaaga ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg
    23521 catatcgata aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa
    23581 gtggcgcagc aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc
    23641 ataaaaatta cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac
    23701 tgtttcaaag ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg
    23761 ttgttttggt gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt
    23821 cccatattcg cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg
    23881 cataccatag caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc
    23941 aaatgcattc tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt
    24001 catcgctttt acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag
    24061 atcacgaata cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat
    24121 cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa
    24181 ttttatctca aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat
    24241 cgatgatatg attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga
    24301 tatattcctg taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg
    24361 cttcttcacg atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg
    24421 tatccagtaa cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat
    24481 cgacatagac agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt
    24541 gaagcgtatt gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt
    24601 ctggaatgat atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag
    24661 attttacacg gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt
    24721 tacggtccat gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg
    24781 tgatcagctg aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg
    24841 ctgttgtact ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt
    24901 ctacgtattt atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat
    24961 aaagataaat cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc
    25021 ggtcattcaa acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac
    25081 gataaaattc tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat
    25141 tagcctcctg cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca
    25201 ccacttaaga gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc
    25261 ttattcttga cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag
    25321 aaggatggaa gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt
    25381 tgaacaatgg gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt
    25441 attttcacgt gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga
    25501 agagttacct gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt
    25561 agctttggca tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt
    25621 aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc
    25681 gaatatctat gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat
    25741 ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa
    25801 gggcggtaca ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa
    25861 aatggaagaa acatttggta atgcagaaat c
//
LOCUS       DADXTH010000040        25687 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.040, whole genome shotgun sequence.
ACCESSION   DADXTH010000040 DADXTH010000000
VERSION     DADXTH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.040"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            349..1818
                     /locus_tag="IWI97_001560"
     CDS             349..1818
                     /locus_tag="IWI97_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463060.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTGNPKNIRLFL
                     YNLFNSTEISFVELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAEQAD
                     SLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETI
                     EWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFREN
                     YPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSY
                     GAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRA
                     VDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1911..2645)
                     /locus_tag="IWI97_001561"
     CDS             complement(1911..2645)
                     /locus_tag="IWI97_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBC2463061.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            2971..3381
                     /locus_tag="IWI97_001562"
     CDS             2971..3381
                     /locus_tag="IWI97_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBC2463062.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            3374..4075
                     /locus_tag="IWI97_001563"
     CDS             3374..4075
                     /locus_tag="IWI97_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBC2463063.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            4075..5688
                     /locus_tag="IWI97_001564"
     CDS             4075..5688
                     /locus_tag="IWI97_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463064.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            5678..5899
                     /locus_tag="IWI97_001565"
     CDS             5678..5899
                     /locus_tag="IWI97_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBC2463065.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            5896..6657
                     /locus_tag="IWI97_001566"
     CDS             5896..6657
                     /locus_tag="IWI97_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBC2463066.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            7038..7547
                     /locus_tag="IWI97_001567"
     CDS             7038..7547
                     /locus_tag="IWI97_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBC2463067.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(7617..8156)
                     /locus_tag="IWI97_001568"
     CDS             complement(7617..8156)
                     /locus_tag="IWI97_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBC2463068.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVAVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(8296..8907)
                     /gene="rpsD"
                     /locus_tag="IWI97_001569"
     CDS             complement(8296..8907)
                     /gene="rpsD"
                     /locus_tag="IWI97_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBC2463069.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            9169..10020
                     /locus_tag="IWI97_001570"
     CDS             9169..10020
                     /locus_tag="IWI97_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBC2463070.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            10048..10683
                     /locus_tag="IWI97_001571"
     CDS             10048..10683
                     /locus_tag="IWI97_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBC2463071.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            10703..11476
                     /locus_tag="IWI97_001572"
     CDS             10703..11476
                     /locus_tag="IWI97_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBC2463072.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEKQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            11884..12681
                     /locus_tag="IWI97_001573"
     CDS             11884..12681
                     /locus_tag="IWI97_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBC2463073.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            12659..13072
                     /locus_tag="IWI97_001574"
     CDS             12659..13072
                     /locus_tag="IWI97_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBC2463074.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            13056..15701
                     /gene="mutS"
                     /locus_tag="IWI97_001575"
     CDS             13056..15701
                     /gene="mutS"
                     /locus_tag="IWI97_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBC2463075.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            15719..17827
                     /gene="mutL"
                     /locus_tag="IWI97_001576"
     CDS             15719..17827
                     /gene="mutL"
                     /locus_tag="IWI97_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBC2463076.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     LQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            17849..18409
                     /gene="maf"
                     /locus_tag="IWI97_001577"
     CDS             17849..18409
                     /gene="maf"
                     /locus_tag="IWI97_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBC2463077.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(18494..20482)
                     /gene="treP"
                     /locus_tag="IWI97_001578"
     CDS             complement(18494..20482)
                     /gene="treP"
                     /locus_tag="IWI97_001578"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBC2463078.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            20705..21418
                     /gene="treR"
                     /locus_tag="IWI97_001579"
     CDS             20705..21418
                     /gene="treR"
                     /locus_tag="IWI97_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBC2463079.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            21495..21941
                     /gene="msrB"
                     /locus_tag="IWI97_001580"
     CDS             21495..21941
                     /gene="msrB"
                     /locus_tag="IWI97_001580"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBC2463080.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            22111..22713
                     /gene="ruvA"
                     /locus_tag="IWI97_001581"
     CDS             22111..22713
                     /gene="ruvA"
                     /locus_tag="IWI97_001581"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBC2463081.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            22726..23727
                     /gene="ruvB"
                     /locus_tag="IWI97_001582"
     CDS             22726..23727
                     /gene="ruvB"
                     /locus_tag="IWI97_001582"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBC2463082.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            23756..24334
                     /locus_tag="IWI97_001583"
     CDS             23756..24334
                     /locus_tag="IWI97_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2463083.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            24386..24868
                     /locus_tag="IWI97_001584"
     CDS             24386..24868
                     /locus_tag="IWI97_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBC2463084.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            24985..25359
                     /locus_tag="IWI97_001585"
     CDS             24985..25359
                     /locus_tag="IWI97_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463085.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         8463 a         4538 c         5441 g         7245 t
ORIGIN      
        1 cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga taacaaatag
       61 taaattttta ttattgtagg atggttgggc tctttttata ataatataaa atataccatt
      121 ttattaataa aaatgcatac tagtaaatga tataattctt ataacaggtt tgatcgcgag
      181 aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat gtatattata
      241 caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta tccccagcta
      301 gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat gaattatgag
      361 ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt agaagcaagc
      421 tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc gacatttctc
      481 ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct agagaaaaat
      541 ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa atgttctagc
      601 aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt attggctttc
      661 tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt gagttattct
      721 gtggcttata aagtattaca aagactaaaa agaaatctta aaaaatatca aatttttttt
      781 gaaaagagaa agttgacagg gaatcctaaa aacattcggc tttttttata taatcttttt
      841 aactcaacag agataagttt tgtggaattg tatacgctac ctataattat aaaaacagaa
      901 aaaatgttaa aagatttaaa acagtattat cctctgtctg cttatgaaaa aaagaaatta
      961 ttccactttt tagcaatcaa cctgttggct gaacaagctg attctttaga tattgctagc
     1021 aatcagtcgc tatttttttc ggatatagga ttttaccact taaaaggagt attaggagaa
     1081 aaatccgtta tttttatcaa taagacactc tcttggcttt atcttcatga taaattggat
     1141 caaaaatact tgagaaaaaa tgaagatgaa acaatcgaat ggttgaatga acgattcatt
     1201 gaaagttttg aaaatcagtt taatctgttg cgagaagaga cgagccaatt gatccgaaga
     1261 gagttaacca aaatacattt taaacttttg cattacacta ttaataaagc tgatgaattt
     1321 ccacttgatg tttctttttt tagagaaaac tatccagcat tttattgcta tttgatagag
     1381 tatataaatg atctaacgtc aaaacgcaaa gagttgcttc aagataaatt ttttcttttt
     1441 ttcagttatt tattattatt gatcaatcac atacccattc aactgatttc tgaaccagta
     1501 aagatcatca tcgatttttc ttatggtgct gcctataatc aatttatcaa gaaaaattta
     1561 agcttgtaca ttaatctgaa cacggaagtg atagaggcag gaaagcctga gttcccggat
     1621 gttataatca cgaataggaa taatctatat gaagaaagtg catctcaagt agtggtttgg
     1681 ctcgatccgc caagagcagt agactggggg aatcttacaa agattttgtt gactatccaa
     1741 gaagagaaat acaaagacag caaaattgac aagcaaatag acgattttcc tgaagaaatc
     1801 atcgacagga tcgaatagtg aacttttttt agaaagtccg actgcttttt ctaaaaataa
     1861 aaaaacttgg tacgcaatcg ctgtaccaag tttttttatt tagcttttga ttaagccata
     1921 aataataatt tatataagaa tactgctacg acaccagcac agataggtgc tacaactggt
     1981 acccatgcat atccccattg agaagaacct ttatgtttca atggaagaag tgcgtgtaca
     2041 agacgtggag caaagtcgcg ggctgggttt agtccaggac ctgtaggacc accaagtgaa
     2101 gcaactaatg tccatacaag gaaaccgagt gctaagtgag ctgtaccaag attatcttgg
     2161 aagaatggtg cttttgtaat acctagggca ccaaagaata aaacgaatga accaaagaat
     2221 tcattgacga aaccattgaa tttagaacca acgctgttga ttgttgagaa tgtacctaac
     2281 acatggttca cgtcttctgt ttggtcgtag taaggtttgt aacaagctac tacgatcaat
     2341 tgtccaatcg ctgcaccaat caattgtgca atgatataag gaacaacttc agcccaaggg
     2401 aatagaccat tagatgctaa agcaattgta aatgctgggt tgatgtggtt accgctgatt
     2461 ccaccaaaaa tcattgctgg catcataacc ccgaatccat acccgacagc aatcagcatc
     2521 cagtcactgc catatccttt tgtacctttt aaatctacgt ttgctacagc accgttacct
     2581 agaacgatta gtaatgctgt accaatcatt tccgccgcaa gacgggtcat tagtgagtaa
     2641 tccataaata cctccaaaag ttataaaaat gcaaaaaaaa agaaacatca acacgaaaag
     2701 catcggtgca gtgtttcgtg tcggaaggat ttctgcagtc ttaacaaatg tgaataaaaa
     2761 aatgaattcc attacttata agaaaccaga ccaacagtaa aactccaact ctttttaaaa
     2821 gttctccaca aatcaccaag cttaactata aatataccaa aaaaaaaacg ttttttttct
     2881 caaaaattgt atcttagaaa cagcatgttt tgaaactttc agaaaaggtt tgttagtaaa
     2941 atgaaagcta tacgtaagga gtgttgtttg atgaaatttt ttcaagagtt tttcattatt
     3001 attttctttt ctgtattagg agaaggcata cggatttttg ctcatttacc tattcctgga
     3061 agtatcttag gtattctttt tttattttta gcatttgaaa tgcatcttgt tgatcctgaa
     3121 aaaattggag caactggtga ttttttattg aataatctag ctattttatt tgttcctgct
     3181 ggtgtaggtt tattggaata ttttgatgat attgcttcga tatggcctgt tttactggga
     3241 gcagtcgtcg tgtgttccat cgtcacgatg gttgcagcgg gaaaaacggc agaaggtgta
     3301 gaggctttgc tgggctatgt ccgtaagaaa aaagcaggaa gggtatctgt aaacgaaatg
     3361 gaggaaaatc aaattgactg agttttttgt gaatccacta tttggcttaa gtttgacttt
     3421 aggagtgtat gtattatctg ataaattttt gcggaaatat aaactgcctg ttttgaatcc
     3481 tctcgtatta tcgattgttg ttattatctt atttttgcat ttttctggga tcagctataa
     3541 agactataat atcgggggca attttttgac tatgctgatt actcctgcga cagtggcact
     3601 tgccatccct ctttataaaa acttccgttt actgaaagag aatttttatc ctgtaatggc
     3661 tgcgatttta gcaggaatag tcgcaaattg cttggttagt gtagggttcg gttacttctt
     3721 ctctcttcat aaagagatgg tgatctcatt actacctaaa tctgtaacga cggctatttc
     3781 tgtcgattta tctcatacga tgggcggggt gaatgccgtg acacttgcta ttgttgtctc
     3841 tacaggtatt tttggttcgc tgatcgccac tcatattttc cgcctattca agatcgaaag
     3901 tccagttgct cgcggtgtgg cgttaggaag tacttctcat gctatcggta cagcaaaagc
     3961 aatcgagatt ggcgaaatag aaggaatcat ttccggactt gcgatatgcg taaatgggat
     4021 attgaccgtt ttgttgctgc cgttattctt tcagcctttt gctggcttgt tttgatgcga
     4081 aaaccactat tttatgctac aataaccaaa acgttcgagc ataaaaaagg agggaaagac
     4141 gtgtatttgt cttccgagta tcttaaacaa tttgataaag atctatatta taagattctc
     4201 ctcctagaaa gttttgagga tcaagcttgg cataccgctg cacaattagc gcaagttgtt
     4261 cagctggatg cccgcagcgt gtcgaaatac ctgaacgagc tgtctaaaaa ctatcagcag
     4321 tttagcggta agacacaccc tttgtttaca aaaaatcacc gttctggtta taacttttat
     4381 gatacgctcg attcaattga acatgagcgt tttttgatct atctcgtgca aagtacgctg
     4441 aaattccagt tgctgcacga catttttttt gaagaattcc atacaatgta ccaatttgcg
     4501 caaaagcatt acattagtga atctactgct catcgaaaaa tcaatgaatg gaaacagcaa
     4561 ctgcaaactt acggaattcg gctgcaacga ggtacatata tcgctcaagg cgaagaagaa
     4621 atcattcgac tttacttgca tatgacattc tggcagttgt ttcgagggaa aatctggcca
     4681 tttgaaacga tttctcaaat ggacgtaaag aatatggcag aacatatcat ggcttttttt
     4741 aatgttcgtc tgaacgagat caaaaaaaga cgattggaat acatgctagg tgcctttttt
     4801 ttaagaaaat cacagaagca ttatgttgta ttgaatgaga aaaaaagacg gctgatttct
     4861 gacaatctgt tgttccagcg attttgccaa gtcatggagc cggtatttcc taattatttt
     4921 caagtagaag acgagttagg cgcattattt cttgtactga tgactcgtga agaatattat
     4981 agtgatccta agatacggaa gaaaattttt gattttcatc aacaagcaaa aacaccgccc
     5041 tttacagccc tttcagaagc aaaagccgct ctttctcttt atcaggaaga gcaggggctg
     5101 ccagcagaaa atctaacctt cgaggcagaa aactatttat tcagcagtca ttttttcgcc
     5161 tatctttttc ctaatgcgaa agaaacgatt gatggcaata gtagtgattt tattaatcat
     5221 ttagttattg aaaacaagga attgaaacaa tggctcgtac atttttttga aagtagacac
     5281 aaacatccca atcatctggc atttaaaaat catgcgtttt tgatgggtcg gtatctgact
     5341 gtgtttaaaa cattaggagc ctttactcca cagcttccaa agatcacgat ccttttaatg
     5401 acagatttcc cattatttga agagcaactg ttagaggagg gattacggac atttttccgt
     5461 aatgagtatc agctgatttt tctgccaaca gattatcggg ggagagaagt tgatcttctg
     5521 atttcgacaa gtaaagtcca ccgcaaacca tgggcggatc ttgactattt tattgtgacc
     5581 gaagaactaa aattaattga ttacatccaa ctatctcaaa aatttgaaat gattcaaaaa
     5641 aaaactatta aaacaataac aaagaaggag aaaaaagatg acatttgaag aaattttacc
     5701 agaattgaaa aaaggtgcaa agattattcg taaaggatgg agtggatttg aactttatgt
     5761 tgttctcgta tccgatgatg aatttgatgg ctgtccagtg acaccttact tattgatcaa
     5821 aacaagtgac gaaggttttt ccagcttcgc tcctacagtt tgtgatattt tagcagatga
     5881 ttgggcgatc gtagaatgaa aaaccagtat ccagatctag ctaaacaggt tgtatttatc
     5941 acaggagctg cttctggaat tggtgctgcg caagcccgag cgtttcttga acaagatgct
     6001 tttgtatttg gattagatca aaagttcggt aaaatggaag aactctcttt gcgatttcct
     6061 gaaacttttg cctacgcact aggtgatgtt cgacaaatga aagaactcca acaagcggta
     6121 tcgaaatgcc aggatcgttt tggtgaagtt acgattttac tgaatacatc ggggatattg
     6181 gatgcctaca aaccattgat ggaaactgac gaatcgctgt gggatcttat ttatgagacg
     6241 aatgtgaaaa gcatgtatca gctgacaaaa atcatccttc ctgatatgat ccgccgaaaa
     6301 agcggaacaa tcatcaacat ggcttcgatt gcgggtttgg ttgctggcgg aggtgggatc
     6361 gcttatacaa gtgcgaaaca tgcaatcgtc ggatttacca aacaactcgc tctggattac
     6421 gcaggacagg gtatccgagt aaaaggaatt gctccaggag ccatccaaac accaatgaat
     6481 gcggctgatt ttgaagggaa aggtgagatg gctgagtggg tcgcaaatga aacaccagtc
     6541 aagcgatggg cacagccgga agaagtagct gagctgacgt tatttcttgc ctctcctcaa
     6601 gcaagctaca tacaaggaac gatcatccca atcgatggcg gatggttgtt aaaataacct
     6661 tgtgtttttt tcaagcttct gtatgataaa actaaagaga gaaacaacgg ttgatgataa
     6721 aagaaagtcg atgactgttc ccgacattcg atgccgaaca gccagttcaa aaaattcttt
     6781 ttaataatcg ttaatattcg aaactaagcg gcttttcacg accttttgat ataaacggtg
     6841 ttcaaaagct tgcaactgcg gtaacaagtc cagccaaatg aaaaatataa ggagctattt
     6901 ttatttgact attcttatta cttgttgcag ggcagctttt tcactacctt ctgcggcact
     6961 tcagttggaa ttttcttccc atcctttggt cgcagtcatt accaagataa aattatcttg
     7021 caaaggagaa atagaacatg gaaaaaaata atacccgttc agccaaacgc tggacaacga
     7081 cagatgtgac tcggatggcg ctagtcactg gtttatatgt ggcagtgacg ctggttttgt
     7141 ctgtctttag ctttggagtg attcagattc gtctctcgga gatgttcaac tatctgtcct
     7201 tgtataacaa aagatacatc gtcgcggtaa cagcgggagt agctttggct aatatggctt
     7261 ctcctcttgg actgatagat gttgtagtag gaagcgtcag cacgctgctt gtattgctta
     7321 tcaactataa aatcactagt cgcatcaaaa acatgaaaat caaaatggct gtgacagcat
     7381 gcgtatttgc tttttccatg tttacagtag ctggtcagct aacgatttta tatcaactac
     7441 cttttttctt gaattggtgg gtgattgctt taggagaatt ggcatcaatg attgtcggtg
     7501 ggataattat ttattggatc ggtaaaaaag tggatttgtc aaaataaaga tctgtttaat
     7561 ttaaaatatg gcagcgtgga agagactttg agtcttcttc catgctgctt ttttgattaa
     7621 tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata agctgctcca
     7681 gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa tgcagttgta
     7741 aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc agcaagagag
     7801 cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc gatcatccaa
     7861 ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg cccaaataaa
     7921 acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc aagtaaaaga
     7981 taaactgcaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt taatccaata
     8041 gcaaatgttt gccccgtcaa aggaaccaag ccaaaaggaa tggtcatctg agaaaggaca
     8101 gaaataatcg cggcaaattc cgcagcaagt attaattctt tcaatgttcg gttcataatg
     8161 ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa caaagtgatt
     8221 actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg gtctttttca
     8281 agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag cttcgtcgat
     8341 ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca atttttcagc
     8401 gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt ttactgttac
     8461 gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt ggtatgaagg
     8521 gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt gacgtgcttg
     8581 acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt gttctagtaa
     8641 gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta cgaacaagtt
     8701 acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt cagttaattg
     8761 catgccgtat tctgattgtt tgccacggct gttaggtcca tgttgacctg gtgcgtaagg
     8821 gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac gagaaatttt
     8881 ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata agtttttgga
     8941 gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc ttcacctttg
     9001 cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg agttattatt
     9061 tttctgctgc attattttac acaaactaca gtatatgata gatcatccac gaatgtcaag
     9121 ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat gagagggtat
     9181 gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc ggtatcacca
     9241 ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga aaacagcttt
     9301 tcacgttacg ctgttcgagc ggttctcgcc tgtttatttt tgactttagg aacggctgtg
     9361 gcatttgcaa tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa gattttatac
     9421 gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgctga attaggaaca
     9481 tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt aagtaaggca
     9541 gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt atgtggattt
     9601 cttatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat gttagaatca
     9661 atagcagtaa aactagcaaa acctgcagca acgattttgg tggaagggat gtttgcgaat
     9721 atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgc cgggaaagtg
     9781 atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca cgtgatagcg
     9841 aattttcctg cctttacgct tgcttacttt gcttctcaag gacagacgga tggaatgaca
     9901 gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat tggtggcggt
     9961 ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta tgtggactaa
    10021 aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa aaatgaggaa
    10081 aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg agtacctaag
    10141 ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt atctatttct
    10201 gaagttttag cttttgttta ttattattat gaacaaatcg aaagagagta tcagtatgta
    10261 gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt agtcgtattg
    10321 ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag aaaagtatac
    10381 agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt gtttgaaaaa
    10441 aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta taaacagcaa
    10501 acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa ggaattcttt
    10561 atccaaaaag caattggttg ggcattacgc gattacagta agaccgatgc cgattgggta
    10621 aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag taagtattta
    10681 taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata ttgatggaac
    10741 gttgttagat tcaaacaaac aagctttaga tagcacgaga gaagcattgg aaaaattacg
    10801 caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg cgcaagagat
    10861 cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg gttttttaga
    10921 ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt tcgttcaaga
    10981 agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca agcgttcttc
    11041 ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg tcttgcctga
    11101 attagatcag ttttttgcag agaaacaaga aatataccaa gcgttagcat ttttcgatca
    11161 aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct ggcatgagaa
    11221 cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc acttagcaga
    11281 acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa acgaccgtga
    11341 aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag aagtacaagc
    11401 agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg cattgaaaga
    11461 gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta ataagcccag
    11521 ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg cagatgcaag
    11581 cttttgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca agaaataaga
    11641 agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt tctgaagaag
    11701 tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt catagtccca
    11761 tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt ttgtttgacc
    11821 ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga agaggaattt
    11881 agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga gatgattact
    11941 gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt aaatggtgag
    12001 aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt gcaagacggc
    12061 gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt tgagtttgta
    12121 aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc tggacaaggg
    12181 atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca ggctcgttct
    12241 tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga agaagccaga
    12301 aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga aaaacaagca
    12361 atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac acacgttcag
    12421 acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt tgggatgaca
    12481 ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa gtttctgacc
    12541 gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag cggctgtatc
    12601 ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca gatcaacgat
    12661 gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc cggaaattca
    12721 aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca ttcgctacaa
    12781 agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag aaaaaatcaa
    12841 acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag cagaacaaga
    12901 agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc ttgttgtagc
    12961 ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa ctatgataca
    13021 agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact aaaaatactc
    13081 ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt ttgttctatc
    13141 gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca cagcttttgg
    13201 aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg tgcggtgtac
    13261 ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat aaagtagcga
    13321 tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc gaagtgatcc
    13381 agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa gacaacaatt
    13441 ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc gatttaagta
    13501 cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat gaagcctctg
    13561 cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt cgtgaacagc
    13621 taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa aacgcggaat
    13681 ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc ggcaaactat
    13741 tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa gcagtagaat
    13801 accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta gaattaagcc
    13861 aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat gaaacaaaaa
    13921 cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc caggcaaaac
    13981 aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt gaacgtatcg
    14041 atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt cgagtagctt
    14101 tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa caagtgccaa
    14161 tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg ctttcagaaa
    14221 tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat gatccgccgt
    14281 tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc gatatgtacc
    14341 gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa gagcgtcaag
    14401 aaacaggaat caaaaactta aaaatcggat ttaaccgcgt tttcggttat tatatagaaa
    14461 tcacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa caaactttag
    14521 caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa atcctcgaag
    14581 cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga gaagaagtca
    14641 aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta gatgtgctac
    14701 aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt gtcagtgaca
    14761 agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta ttaggacatc
    14821 aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg ctgattacag
    14881 gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact gttctgatgg
    14941 cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt gatcggattt
    15001 ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt atggtagaga
    15061 tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt ttgtttgacg
    15121 aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca atcatcgaat
    15181 atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat gaactgacgg
    15241 tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt gaaaaagacg
    15301 gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc tacggcatac
    15361 atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca gatatcctgc
    15421 atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta gaagagaaaa
    15481 ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat caagaccata
    15541 tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa ttatccgaaa
    15601 atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg acgccaatgg
    15661 atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc ggatatcgat
    15721 ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg gagaagtagt
    15781 ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg ccggcagtac
    15841 tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga ttgataacgg
    15901 agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga caagtaagat
    15961 ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg aagctttgcc
    16021 aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag aaaaacaagg
    16081 cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac cactgcgtca
    16141 aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct tgaaatacgt
    16201 gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac tggcattgag
    16261 tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg caactgctgg
    16321 aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg caaaaaaaat
    16381 gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt cattgcctga
    16441 ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt ttatcaagaa
    16501 cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg tcggtcgttt
    16561 ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa atgttcatcc
    16621 gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa ttagtaacgc
    16681 cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata atctacggtt
    16741 taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt tgtcctacgc
    16801 ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag agacaggtac
    16861 ctttttcgta gaagaaccaa agaacacatt tgttgacaga attgttgata agtctgtgga
    16921 aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag aaaaaacagc
    16981 gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa cagtggaaaa
    17041 atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg aagaacaagc
    17101 agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac tccatcctga
    17161 atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga aattagaaac
    17221 ggaaaaacca gtacaacgtt ttcttcagtt ggaatatttt ggacagatgc acggaacgta
    17281 tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg cccaagagcg
    17341 cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc tgcaagaatt
    17401 gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa aagagcaaaa
    17461 acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca gctttattgt
    17521 acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc gggagatgat
    17581 cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag caacagcaat
    17641 catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag cacaagcacg
    17701 tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc atggccgtcc
    17761 agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa tccaagaccc
    17821 gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc aatcaccaag
    17881 aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg ctgatatcga
    17941 tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg ctgaagaaaa
    18001 agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg ccagtgacac
    18061 gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg aggctgaatt
    18121 catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg ttttaaaaac
    18181 aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc ctttgacaga
    18241 cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag gggcatacgg
    18301 catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt acagtatcgt
    18361 aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa caaaaaagcg
    18421 gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg aaaagattac
    18481 ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc gggcgaacaa
    18541 gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa gcaacaataa
    18601 tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca atcagggtct
    18661 ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc tttagttcaa
    18721 cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga ataccaatgg
    18781 catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag actttgccgt
    18841 ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact gggtcattta
    18901 cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct tttgttgcaa
    18961 ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt gcgccatttg
    19021 ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc ttattcaaaa
    19081 ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca atgatcattg
    19141 cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc aaaccgccta
    19201 ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct aatccagagc
    19261 cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac atagcaggtt
    19321 cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct tcttttttat
    19381 tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata tttgataaag
    19441 caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata tcgatggcat
    19501 tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg aatacggcac
    19561 taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag atccagcttc
    19621 cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt ggtaacaatg
    19681 aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg aagaagattt
    19741 ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct tgataaccaa
    19801 tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg gctgtggaag
    19861 ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa acgattccta
    19921 atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact ggaagaaaat
    19981 ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa aacttcgagt
    20041 gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca tttctgaatc
    20101 caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt tctgctaaaa
    20161 cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt ccttgttctt
    20221 tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta gaaacatcgt
    20281 ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg ctaggtatat
    20341 cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc atacgagtcg
    20401 cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac agcttctcgg
    20461 catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt ctatgcaagt
    20521 tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta ttaaaaagta
    20581 cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa aataggaaca
    20641 ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga gtgagggaaa
    20701 aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt tagcgaaaga
    20761 atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt atggtgtttc
    20821 acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata ttcaaaaaaa
    20881 acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta tttctggatt
    20941 gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga tcatccatac
    21001 gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg agatcggtgc
    21061 gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta tattagacac
    21121 cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac aaggatcgat
    21181 ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa aggagatcac
    21241 agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg acacttatgc
    21301 agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt attctgagtc
    21361 ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa aagtgtaaat
    21421 agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat aggatcacaa
    21481 agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg aaagaaaagt
    21541 tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca tttagcggga
    21601 aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt gaaccgttat
    21661 tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc aaaccaataa
    21721 cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga atagaagttc
    21781 gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca gtagatcaag
    21841 gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat gaaaagatgg
    21901 atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac acaaagagcg
    21961 gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga ttgctatttg
    22021 aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa taaagggaac
    22081 gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg tgtggttacc
    22141 tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca gatcgcacta
    22201 ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta tgtccatcag
    22261 gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga aaagcaactg
    22321 tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc aatcatggca
    22381 agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac ttatttgacg
    22441 aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct aaaaggaaaa
    22501 ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa cacaaaagac
    22561 gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag tgatcgagaa
    22621 atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga tgagtatcta
    22681 cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac agatgaatac
    22741 ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct tcgaccacag
    22801 ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat ttacatcgaa
    22861 gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc tcctggatta
    22921 ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat tcgtacgacg
    22981 agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga actggaagca
    23041 ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga agagatgctt
    23101 tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc aaccgcacat
    23161 ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg ggcaggtatg
    23221 ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata ttatgacgaa
    23281 caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga gatcattgaa
    23341 gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc taatcgcttg
    23401 ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga tcgtcctata
    23461 gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta tgttgaccag
    23521 aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt aagtacgctt
    23581 tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc gtatctgatc
    23641 caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta tgcctatgaa
    23701 cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa aatgaatgag
    23761 tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt gccaaacgca
    23821 atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca atcgacaaac
    23881 attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt gtgaggatgc
    23941 attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc aagcattgaa
    24001 aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc tttctgaccc
    24061 agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca agatttttga
    24121 acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg ctcgtttttt
    24181 ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg aagaaacacc
    24241 gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat attcttatcg
    24301 tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt tggaaaagca
    24361 ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt tgggaaatat
    24421 ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag aaggaaggga
    24481 agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa agacaccgca
    24541 ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata tggttgctcg
    24601 gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg atcaagaaaa
    24661 tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc atttgttttt
    24721 aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata ccaataactt
    24781 tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata tttggattga
    24841 tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg cgatgttaga
    24901 gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag taacactttc
    24961 ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat gaagaagctt
    25021 acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa tgacactgaa
    25081 gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca aaaagctgct
    25141 actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag caagtatgaa
    25201 acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat gatccgccta
    25261 caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa aataacaatc
    25321 ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga tagaaaagca
    25381 cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct ttaagctgat
    25441 atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc ggttgattag
    25501 ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta gccggggagc
    25561 tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt ggtatgctta
    25621 tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt gttaagtttt
    25681 tgagagt
//
LOCUS       DADXTH010000041        25675 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.041, whole genome shotgun sequence.
ACCESSION   DADXTH010000041 DADXTH010000000
VERSION     DADXTH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.041"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            170..514
                     /locus_tag="IWI97_001586"
     CDS             170..514
                     /locus_tag="IWI97_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBC2463086.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IWI97_001587"
     CDS             515..934
                     /locus_tag="IWI97_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBC2463087.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="IWI97_001588"
     CDS             complement(1026..1376)
                     /locus_tag="IWI97_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463088.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IWI97_001589"
     CDS             complement(1342..2673)
                     /locus_tag="IWI97_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBC2463089.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IWI97_001590"
     CDS             2929..3495
                     /locus_tag="IWI97_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2463090.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IWI97_001591"
     tRNA            3640..3727
                     /locus_tag="IWI97_001591"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /gene="bsh"
                     /locus_tag="IWI97_001592"
     CDS             4061..5035
                     /gene="bsh"
                     /locus_tag="IWI97_001592"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBC2463091.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IWI97_001593"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IWI97_001593"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBC2463092.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IWI97_001594"
     CDS             complement(7450..7896)
                     /locus_tag="IWI97_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBC2463093.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IWI97_001595"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IWI97_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBC2463094.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IWI97_001596"
     CDS             8361..8879
                     /locus_tag="IWI97_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBC2463095.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IWI97_001597"
     CDS             complement(8956..9912)
                     /locus_tag="IWI97_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2463096.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IWI97_001598"
     CDS             10078..10884
                     /locus_tag="IWI97_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463097.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IWI97_001599"
     CDS             10881..11870
                     /locus_tag="IWI97_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBC2463098.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IWI97_001600"
     CDS             11867..12871
                     /locus_tag="IWI97_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBC2463099.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IWI97_001601"
     CDS             13003..13545
                     /locus_tag="IWI97_001601"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBC2463100.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IWI97_001602"
     CDS             13565..14263
                     /locus_tag="IWI97_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBC2463101.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IWI97_001603"
     CDS             14284..14496
                     /locus_tag="IWI97_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBC2463102.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IWI97_001604"
     CDS             14496..15458
                     /locus_tag="IWI97_001604"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBC2463103.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IWI97_001605"
     CDS             15476..15871
                     /locus_tag="IWI97_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBC2463104.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IWI97_001606"
     CDS             16034..16399
                     /locus_tag="IWI97_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBC2463105.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IWI97_001607"
     CDS             16396..18207
                     /locus_tag="IWI97_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBC2463106.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IWI97_001608"
     CDS             complement(18269..18436)
                     /locus_tag="IWI97_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBC2463107.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IWI97_001609"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IWI97_001609"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBC2463108.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IWI97_001610"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IWI97_001610"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBC2463109.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IWI97_001611"
     CDS             21193..22173
                     /locus_tag="IWI97_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2463110.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22364..23086
                     /gene="tsaB"
                     /locus_tag="IWI97_001612"
     CDS             22364..23086
                     /gene="tsaB"
                     /locus_tag="IWI97_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBC2463111.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IWI97_001613"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IWI97_001613"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBC2463112.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IWI97_001614"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IWI97_001614"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBC2463113.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IWI97_001615"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IWI97_001615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBC2463114.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8328 a         4400 c         5377 g         7570 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctggatgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tattt
//
LOCUS       DADXTH010000042        24940 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.042, whole genome shotgun sequence.
ACCESSION   DADXTH010000042 DADXTH010000000
VERSION     DADXTH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.042"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(8..2152)
                     /locus_tag="IWI97_001616"
     CDS             complement(8..2152)
                     /locus_tag="IWI97_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBC2463115.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFNHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2509..3882)
                     /locus_tag="IWI97_001617"
     CDS             complement(2509..3882)
                     /locus_tag="IWI97_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2463116.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3882..4277)
                     /gene="rbsD"
                     /locus_tag="IWI97_001618"
     CDS             complement(3882..4277)
                     /gene="rbsD"
                     /locus_tag="IWI97_001618"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBC2463117.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4305..5222)
                     /gene="rbsK"
                     /locus_tag="IWI97_001619"
     CDS             complement(4305..5222)
                     /gene="rbsK"
                     /locus_tag="IWI97_001619"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBC2463118.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5330..6343)
                     /locus_tag="IWI97_001620"
     CDS             complement(5330..6343)
                     /locus_tag="IWI97_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBC2463119.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6424..7023)
                     /locus_tag="IWI97_001621"
     CDS             complement(6424..7023)
                     /locus_tag="IWI97_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2463120.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7209..7910
                     /locus_tag="IWI97_001622"
     CDS             7209..7910
                     /locus_tag="IWI97_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463121.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7920..10631
                     /locus_tag="IWI97_001623"
     CDS             7920..10631
                     /locus_tag="IWI97_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2463122.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11082..12710
                     /gene="treC"
                     /locus_tag="IWI97_001624"
     CDS             11082..12710
                     /gene="treC"
                     /locus_tag="IWI97_001624"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBC2463123.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12770..13951)
                     /locus_tag="IWI97_001625"
     CDS             complement(12770..13951)
                     /locus_tag="IWI97_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2463124.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDYTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14203..15726
                     /locus_tag="IWI97_001626"
     CDS             14203..15726
                     /locus_tag="IWI97_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBC2463125.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15795..16523)
                     /locus_tag="IWI97_001627"
     CDS             complement(15795..16523)
                     /locus_tag="IWI97_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2463126.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16536..17816)
                     /locus_tag="IWI97_001628"
     CDS             complement(16536..17816)
                     /locus_tag="IWI97_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBC2463127.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17957..18559)
                     /locus_tag="IWI97_001629"
     CDS             complement(17957..18559)
                     /locus_tag="IWI97_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBC2463128.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18804..19010
                     /locus_tag="IWI97_001630"
     CDS             18804..19010
                     /locus_tag="IWI97_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463129.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19322..20041
                     /locus_tag="IWI97_001631"
     CDS             19322..20041
                     /locus_tag="IWI97_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBC2463130.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20122..20805)
                     /locus_tag="IWI97_001632"
     CDS             complement(20122..20805)
                     /locus_tag="IWI97_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBC2463131.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21023..23209
                     /locus_tag="IWI97_001633"
     CDS             21023..23209
                     /locus_tag="IWI97_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBC2463132.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23555..23965
                     /gene="spx"
                     /locus_tag="IWI97_001634"
     CDS             23555..23965
                     /gene="spx"
                     /locus_tag="IWI97_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBC2463133.1"
                     /translation="MIKVYTSPSCTSCRKAKAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            23979..24335
                     /locus_tag="IWI97_001635"
     CDS             23979..24335
                     /locus_tag="IWI97_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBC2463134.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8361 a         4429 c         4860 g         7290 t
ORIGIN      
        1 atttattcta tttctttaaa ggcagatcaa gccagaatga tttgtctaaa cttaacaagg
       61 ttagtacagg aacagttgca gctttaactg tacgagtagt tgtttctggt atctgtcttg
      121 ccgtaaaatt gttccacaaa tcccagattg aagcgacacg gaagatggaa gaagcgttgt
      181 tgacgcgttt tatcgttgaa aattctaatc ctgtgattgg cacgccatct gttgtccatt
      241 tgattggcac atcgaatcgc ttagacatat tgaggatttc agcgtttgct tcttcagttg
      301 ttgctgcttc ttttttcggt tcatcatatt ttgtcaggtc gtacgcttcg atgatcgcgt
      361 tcaatttttg tgaatattgg cgatctgtag cataacgtcc ttccaaatat ttggttgctt
      421 ctttgtagtc ttttgtttcc gacttccaag ttggtttgta gaaatcagga ttgccagtca
      481 gtccattttt gatcaagtcg gcatagtctt ccaaactttc cttgtaagaa ggatatttac
      541 ggaattgtgc gcggatttgg tagctttgtc cggaactatc ttgttcaagt gtattgaagg
      601 tcacgctgct tcctttatat gatcctttga taccaaaaag gttatgattt ggttcgcttg
      661 ataataagct gtttccagat ccactttcaa ggatagcttg agccatcatg actgatgcgt
      721 agattccttc tttttgcccg atttcctgtg catcatctgc aatctttttc aagaactgat
      781 cagttgtttg gttttgactg aaatggaacg agccatctaa tccatctaaa atagatgagc
      841 cgcctgtcag cgaacctgaa gcagcaggta aacctgctaa gtttactcgg acagcaaaat
      901 caggcgtgaa atctgaaata gaagttactc gaactttatc tcctggttga ggcgcatgga
      961 taaattgtcc gttcccaata tagataccta catggtgagt aacaccggaa gaaggtcccc
     1021 agaagacgag gtcgccaggt tgggcctgac ttactggaat ctgcgtacct gcatattgct
     1081 gttcgcctgt ccagccaccg atataatgac cggttgcttt catatatacg taataagtca
     1141 aaccagaaca gtcaaaagta tttggacctt ttgctcccca tacatatggt tttccaagat
     1201 gattgaatgc ttctcgaacg attgcttgtt ggacattcgt tgtatttgta ggcaatacag
     1261 catcaattgg tgattttaca acaaaatcag aagaagcaga agatggattc tctataccgc
     1321 tttgatttgc tgtatttgga cgagatgaag cattgttcgt tccgccattc gatgaaccat
     1381 tgtttggatg gtttggttct ctctgagtat ttccattgtt attcgatcca gatgggtttg
     1441 tttgattcga agaattcccg ctgttcgaat tattgttatt attgtttgaa ttatttgaac
     1501 cattattatt gttgttatta cccacatctg gtttgtttgg cgtttccgga tctggtgtac
     1561 tagggacttc ttcactagga tcagttggtt tctctgttga agtatccggt ccagctttaa
     1621 attctgcagt aatagataaa ttgttcgtac ctactgtaaa ggtctttgca tcataattta
     1681 cttctttggc accattaaca gcgactgaag caatttgttc accttcagct ggcgtaatag
     1741 tgtaagttac cttcgtttcc gctaataaac cttctaccgt tccactttcc gtaatatcca
     1801 aaggttgctg ttcaggggaa tccgctgtcg aagcagtcag ttggatctta ccaatcaaag
     1861 aaggagcaat tgtaagggaa tatgtctcgg ctggcttttc attttctgat ggatcagccg
     1921 gatcaatttc agttggctcg gtcgatcctg ttgttgagtt agttgttcca gttgggtctt
     1981 ctgtaaaaga atccgttgtt tcactagtag ctgagctgga tgtagtcgta tctgttgtga
     2041 ctgtttgatc agctatttga ttatctacgg caaatacggt attggtcgac aaaacagacg
     2101 gagcaatcag ccaagtagca gctagcaaac gtatcgtttt tttatttttc ataataaact
     2161 agtccttttc ctttcattga ttttctgtct tattcagcat tcattagaca ttttttggaa
     2221 cagataatcg tgtcgaaaag ctttttcttt tcataaatct gtctgtgtat tctaaataaa
     2281 acaatttgta aataagcaac gagcattcat tcgtttttct ttgacgttca ttttattgca
     2341 tagaccacct ttgtttgggg ataaacaagt ggggataaaa atggttatgc atatgtaaat
     2401 tataccaaag tttggaaagt atcacaaaaa tttataaaaa taatgatcct aaaaagaaaa
     2461 agactgaaat atgtgataaa catctcatac ttcagtcttt ttcattaact attcataaga
     2521 cattaggtct tcatcaaacc cgctaacgag agtagtgatc attgctacga ctagactaac
     2581 agccagtcct gctaagtacc agaaaaaagt accggaaatg aatgcaggca atgtggtcag
     2641 actaccaaat acaaaagcga tcgctttcac ttgggcaaat cccattacag ctccaccagc
     2701 agctgcacca atgcaagcag caacaaatgg ttttttcaat gggatcgcaa ttccgtaaac
     2761 agttggttcg gtcactcccg caagtaaaat cgaagcaaaa gcagaaccgg agatcgaacg
     2821 ggtttttgaa cgtttacttc gcagaaatac acctaaagct gcaccagcca ttccaaaatt
     2881 ggctgctcct gtcaacggaa cgattcggtc gaacccataa gcagagatat tactgatcat
     2941 gacagggttt acagcccatt ggataccaaa tattaccaaa atattccaac caccagcgat
     3001 gattgcgcca ctgatcggtc cgttaatatc catcatccaa gtaattgcgg cagctaaacc
     3061 ttcgccaagg taaacacccg caggcccgaa taccattaac gtcaaaggaa ccgtgataac
     3121 taatgaaagt aaaggtacaa atactaattg catattttcg cgaatatatt ttttgaggaa
     3181 tttttcaaca aaagaataaa agaaaatacc taaaatcgcc ggcatgacag tagacgtata
     3241 ctgcatcagt actacaggaa tccccagaaa atcagtagct gctttgccac tgtctgaaag
     3301 taaagaggtc agattcggtt caatcagtgc tgcagcaatg acagccgcaa tatatccatt
     3361 cactttaaac gttttggcag cagaaaaagc aagaaagatt ggcagaaaat aaaagacggc
     3421 attcgacgca gcagacaaaa tatgataagt tccgcttgtt tcactaagcc agccaatcgt
     3481 agtcgctaaa acaaccagac cttttaatat accagaaccg gcaaacagcg gaatgatcgg
     3541 cgtaaagatt tccgatatca aaccaaatag tttttcgatc ggatttagct tttctttcgg
     3601 agctgaatga tcgatttgac gcgtcaattc cttgtaagca ggagccaatc cctgatccat
     3661 gattatttct gcatatctat cgagtaaaat gaaatactta atggaaggga tttgctgttt
     3721 taatttttct gcattggcta ctggctgttt tttcaatttg atacgaagcc gagtcgcaca
     3781 tggaaggaca gaatcaatat tgtcttttcc tcccaactgt tcaatcagca tatgaatctg
     3841 ctcggacagt accatctgtt caggattcat tgtcgtttcc attagaagac cactccactc
     3901 actaatataa tattggcgta aggtgtcatt tcgcctgtac gaataaatgc atgacagtta
     3961 ttcaactctt ttttcatttc ttcatgtggg ataaagctaa tcggtgtatc tggtaacaat
     4021 aatttgattt tttccaatat ttccgggttc attgtcttta tttcttccgc caattcaatc
     4081 gattgtacct caagttcttc taaaacgttt tctaaaacag ataaaaaact tggaagaccg
     4141 tttgagacag ccaaatctat tttttcagta ccaaagggga caggcatgcc tgcatccccg
     4201 atacttaatt tatcaaagtg ccccatttgt gcgattactc gtgaaatatc tgaattgatt
     4261 acttttcctt ttttcatgtt tgtttcttcc tctcattgtt aattttagac tgctgattgt
     4321 tctaaagctt cgcggtaagg gatagaaggt tgcgcaccat aagactgcac agcaacagaa
     4381 gaggctagat ttccataaag gatcgcttct ttcaagttac taaggtctgg ctgaagtgtg
     4441 ctgcttaatg cgccaataaa tgtatcacct gcggctgttg tgtcaacggc atccacttta
     4501 aaagcaggga cgataccgtg ttctttttct gtatgataga aagcgccggc actgcctagt
     4561 gtaatgatta ctgttccaat acctaattca tggagttttt cggctgctgc gaccaaactg
     4621 ttatgatctg tcactctgat tcctgtaatg atttctgttt ctgtttcatt tggtataatg
     4681 atatctgtct tttcaagtag ttcagtagga atcgtttcac gagctggtgc aggattcaag
     4741 atcgttgttt ttcctgcgtc ttttgcaata gaaaaagcta ggatcgtcgc atctaagcta
     4801 ctttcaaact gagcaataat aaagtcactg tttgtaatca gttgcttgtt ttttaggact
     4861 tcttgttcat ggaaagcatt atttgcccct gcatggatca agatactatt ttctcctgct
     4921 gcgtctacca taatcattgc ttgtccggtt gtggcgcctt ggatttcagc tatcccagat
     4981 gtatcgatgt tttcttttgt cagtagatct aataactgtt gtccttcgct gtcagcccca
     5041 acgcctccaa taaaagatgt ccgtgcgcct gaacgtttgg ctgccactgc ttggttggca
     5101 cctttacctc cgccagcgtg aaaaatctca tgcgcatgca tcgtttcgcc tggcttgggc
     5161 atgttggtta aacgaagggt agtgtccata ttaatacttc caattactgt aaccgtgttc
     5221 attttacaaa cctccaacta tctcatttat tttgatggga aaattatttt ttaaaacgtt
     5281 ttagtaaaac gttttaatag ttcgataaaa atatagcaca ccttgaaagc tatttcaagg
     5341 gtgcagttga aaatcttttt tctaatcgta caggtaatct tttttcttcc cattcttttt
     5401 ctgggaattg gatccgatct aacaataact tggcagatgt ttgtcccaat tcgaagattg
     5461 gttgtgcgat cgttgtcagt ttcggcttga tatactcgca catatctata ttatcatagc
     5521 caataatgct gtagtcttca gggatgcttt ttccagcttc ctctaatccg cgatataatc
     5581 caaaagctaa ttcatcattt aaagcaaaca caccagttgc agcagaatca agaagttctg
     5641 ctgtgatctg gtagccacct tgttttgaga actgtgtagg taagaggatc agttggtcgt
     5701 gagggatttg gtaaacatca agagcagatt taaacccctc aatccgagcg tggacatttt
     5761 ccggtggatt ttcaggatag acaagggcga tcgtttgatg tcctagacta agcaagtgca
     5821 tgcccgctag gtaaccaccg cgaaaatcgt ctgttcgtac ggcatcacta aatccttcag
     5881 attttttctg atccaaaaca atgaagggcc ggccttgttt tttcaaattt tcgttgattg
     5941 catctgtaga gacagcagaa gtcgcgataa tgaatccatc cacacctcga cgtgtgagct
     6001 cggctagata ttcgatttct ttttgatgat cagaatcagc attacataat atcgtgacga
     6061 aattctgctt atacaagata tcctcaattc ctcgcatcag tgtactgaag aatggattcg
     6121 tgatatcagg aacgagtaca ccaattgttt tcgtttcctt ggtaatcatt tgtctagcaa
     6181 aatagtcagg ctgatagcct aattcttctt tggcagctaa aactttctga acagttttcg
     6241 gactgaattt ctccgtattt ccatttaata tcaaggagac agttgtgatt gaaacgccgg
     6301 aaagtttagc gacttctttg attgtgatct tttttttcac catgttcata ctcctatcta
     6361 aatcctgtgg ttattttccc atgaaatcaa aaagaagtaa atgaaaatca cgtgtgccag
     6421 tacttatttg ctttgttgga tagcttcgat tatttgtttc gttgagcgta ctcgtgcagt
     6481 tttcggaaat atctttttta tgaacaattg atgggattct ttgtcacgat cactgcaagc
     6541 atcttcagca agcacgatat ggtacccgtg ttgaaaggca tctaatgcag tagcatatac
     6601 accgtttgtt gtcgctacac cgcttaaaat gatagtttcg attccacgcc ggcgaagttg
     6661 aagatcaaga gaagtaccaa aaaaagcact tggattgtat tttgtgatag tcaacatatt
     6721 atctgtatct ttcaaagaat ccttcaaaag taaatcagtg aattctggag gtacagaagc
     6781 agtcattcca cccatatcag tttgttgact caaataaggg aaagtggaga tgtctacgtt
     6841 cactaaaact ttcaaggcag cggtacgttt caatgttctt actaacaaat cattgtttgt
     6901 cagaatagat gctgcagtgt gtggagagag tgtaccgtta tttacgatct ctttttgcat
     6961 gtcgatcgcg acaaaagcag tttttgataa atcaaaaatc atattttcct ttaaatcatt
     7021 catgatgttc cttctttcaa ataacaatgt tttttatgta agtaaatcat aaatcgttta
     7081 tttgtcaact aaattaaagg attcctttct ttagtctaga gaaattcagt ggtaaaataa
     7141 ataagtgtga gaaaacgcat tcgatgcata gatatagcga gaaatatcgt gaaaatggag
     7201 gaatcagagt gaagtatgtc gaagtaaaaa atgaatacaa acgatacaaa atgggagaaa
     7261 cgaccattac agcaaatgac gatatttctt tttcaattga aaaaggcgaa ttagtgatta
     7321 tacttggtcc aagtggagca ggaaagtcaa cgattcttaa tattttaggt gggatggata
     7381 cacctgatga aggtcagatc attattgatg acacagatat tgcgcaattt tcagataaac
     7441 agcttactgc ttaccgacga acagatgtag ggttcgtttt ccaattctac aatctcgttc
     7501 ctaatctaac agcaaaagaa aatgtggaat tggcaacgga agtttctcct gatgcgcttg
     7561 atcctgtaga ggtattgagg caagtgggcc tagctcatcg attgaataac tttccgtccc
     7621 aattatcagg aggcgaacag cagagggttt caattgctcg tgcactagct aaaaatccca
     7681 aacttctttt atgtgatgaa ccgacaggcg cgctggattt tgaaacgggc aagcaagtat
     7741 tgaaattatt gcaaaacgct agcagacaac acggcaatac cgttttgatc attacacata
     7801 attctgcttt agcgcccatc gctgatagag tcattcacat taatgatgct aaagtaaggt
     7861 ctgttgaatt aaatgatcat ccttcatcca ttgatgagat tgtatggtag ggggatttta
     7921 tgaaaacaag aacatatctt aaagcaggtt ttcgcgaaat tcggcaatct aaaggacgat
     7981 tcattgcaat catccttatt gtcatgctag gaacactttt atttgtaggt gtcaaaactg
     8041 ctggacctgt catgcaaaaa acgatggatc actatgttgg tacagcagga ctttctgatt
     8101 tgcaaatcgt ttctactggt ggcttgacac aaaaagatat tgctcaagca gaaaagatcc
     8161 cagatgcaaa agtagaaaca ggaaaacaga tctattattc caatactggc aaaaacgaag
     8221 tgattcaaat attttcttat aacaaaaata acaagcagaa caagctacaa ttaactgatg
     8281 gaaaactccc ggaaaaacca aaccagcttg tattggatga aaaagcggaa gatgaagggt
     8341 ataaaatcgg tgatatttat cagattgatt cagatgaatt gaaagaaaaa gagtatacca
     8401 tcacgggctt tgtccgttct ccattgttca tcaacaattt ggagagagga tacgcgaacg
     8461 tcggaaatgg tagcgtcgat tattttgtct atttacctga atccgttttt aaaacagata
     8521 tttattctgt cttatatctt gattttacaa acgtaaagaa tttgaatacg tacagcaaag
     8581 cttacaaaga taaaatggag caaaatcaag aaaaggcaga aaagtattta caagatcgac
     8641 ccgaagaacg cttagaagag ctgaaaaaat cggccaacga agaattagaa ccggcacagc
     8701 aaaaaatagc ggatggcaaa gcacaaacaa cgcaagcaag aacacggtta gaagatgcta
     8761 aaaaacagtt agcgcaacag tcagcaacta tccagcagct tcctgaagcg caacgaacaa
     8821 tcgctgttca aacattatct caacaagaag ctcaacttgc tgaacaagaa aggcaattag
     8881 ttgagaaaga acgtgaactg gcctctgcag aggaagaatt gaatcgaaat gcagaaaaaa
     8941 ttgaaaattt aactaaacca tcatacctat acaatgaacg ttcagaaaat gtcggttttc
     9001 aagaatttgg tgatctgtct gatcgtatcg ctgcgattgc caatgttttt cctgttttct
     9061 ttttctttat tgccgcattg atcaccttta cgacaatgac acgtatggta gaagaaaacc
     9121 gcagggaaat cgggacatta aaggcgttag gttatacgaa agttgaaatc gcaaaaaaat
     9181 atgcaatcta tgcaagtcta gcctcaggga taggtattgt tttaggaacg atattaggaa
     9241 cgaatctttt gccaaggatc atttttgagc tgtctaacga gcggtatgat attgggagtg
     9301 cgcttatttt ttatgactgg ccgccgattt tacaagcagc tgccgcattc ttcattgccg
     9361 catttggcgc tgcaatgatc gttttattca aagatttaag agaacggcct gctgcattgc
     9421 ttcaaccgaa agcaccgaag ccagggaaac gcatttttct tgaatacatc actccgctgt
     9481 ggtcacgcct tagctttaat caaaaaatca gttaccgaaa tctcttccga tataagtcaa
     9541 gaatgcttat ggcgattatc ggaattgctg gatgtgccgg gctgatggtt gcaggcgtgg
     9601 gattgaaaga ttctctaagt tctgtttcag ccaaacaatt tggaacgatc attgactatc
     9661 aagcaatcgt tacattggat tcatccacgg aagatagtcg tcaaaaagca gcggatatgc
     9721 tgcagaagca agaaaaagta aatgagatcc tagctattca tactcaaacc gtagaattga
     9781 gaaaaaaagg acatgcttcg caaagtctga cagtgatcgt accaagtaaa acagctgatt
     9841 tggatcgttt tatccattta actgatcttg aagagaaccc acgctccttg cctaaaacag
     9901 gaatcgctat tacgcaaaaa gtagcagaac tatttgactt atctacagga gatcatattt
     9961 cgctttatga tgaagatcaa aaagaatgga aagtcgaagt aaaagaaatc gtgaaaaact
    10021 atctaggtag ctttatttat atgtcccctg aatattatgc ttcagttact gatcaatcga
    10081 tgatggataa tgcatttttg ctgcgtacag ataagatgac aacaaaagaa gaagaaatgc
    10141 tttccgagaa actgcttgcc acagacgctg tgacaaatac gtctttcatg tccaaacaaa
    10201 tcgaaacgca agaagaatca atggcgaact tagactcggt tgtcttaatc tttgtcgtat
    10261 tatcaggact gctggctttt atcgtcttgt ataatctaac taatatcaat atttccgagc
    10321 gtgtacgcga attatctaca ataaaagtcc ttggattttt tgataaagaa gtcacgatgt
    10381 acattgttcg agaaaatatc atctttactc ttttaggaat catcggagga tttggtgtag
    10441 gatatatatt gactgacttc atcttgcaac aagcgtctat ggagaatgtc atctttccat
    10501 tagtgatcac atggtgggct tatgtcttat cagcaggatt aactatcgta tttacggtta
    10561 ttgtgatgat tgttacccat ttcaaattaa agcatatcga tatgattgat gcgttgaaat
    10621 cgaatgaata aaaacagagg ttgtgaaatc agcggtttga cctgagtatt agagaaacaa
    10681 acggcaaaat agtttttcat attttgccgt tttttgagct aataccgaag gacagctgat
    10741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    10801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    10861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    10921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    10981 taaattttac aaacgctttc ggttcagtta taataataaa tgataacttg tatatataag
    11041 ttgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    11101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    11161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    11221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    11281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    11341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    11401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    11461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    11521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    11581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    11641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    11701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    11761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    11821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    11881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    11941 attttgcaga attaaagcga ctatttcata catggggaga aaagatgagt gagggtaacg
    12001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    12061 ctatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    12121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    12181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    12241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    12301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    12361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    12421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    12481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    12541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    12601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    12661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    12721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    12781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    12841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    12901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    12961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    13021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtatagt
    13081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    13141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    13201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    13261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    13321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    13381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    13441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    13501 cgtcaataga agcgaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    13561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    13621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    13681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    13741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    13801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    13861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    13921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    13981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    14041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    14101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    14161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    14221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    14281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    14341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    14401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    14461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    14521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    14581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    14641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    14701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    14761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    14821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    14881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    14941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    15001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    15061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    15121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    15181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    15241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    15301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    15361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    15421 tttacgttta agaatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    15481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    15541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    15601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    15661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    15721 cactgatagg caaaaaagaa ccgaagggtt tggatcggct ggtcatttag tcggtccaaa
    15781 ctattcggtt ctttttatga aatcaatttc tttaatccac ggatagaggc tgggatggtc
    15841 tcgccgtttg ccaacgtgac ttctctggtt tttttattaa tcgaccgcac attttgtgta
    15901 ttgataacat aggatttatg acaacggata aaattccgat gcattttctc gatttctttg
    15961 atcttctcat agaattgtaa ttgggcatga gtcaaatgga cttccagttt atgtggcgct
    16021 cctagcgaag tagaaaaata ttggatgtct ttgatcggta aaaatttgat ctcatcatta
    16081 aactctattt tgaagatatc tttttggatt tgggacattt gaaaatcttc gaaaattttg
    16141 ctcaggatat gctcgacatt ttcttttaaa tcataaagat cttctttgac gatataatca
    16201 gtcggttcga tgttgctttt taagattttc atcgacattt ccgtatggga agtcacatag
    16261 attatttttc ctaaaggatc taattcacgg atcaatttac cgatcgctac accatccatt
    16321 tcactgtctt ctagttcgat atctaaaaag taaataccaa aagaagaagt tctgatcttg
    16381 gctgcattga gaagttcttc cggatcagag gtagaaatat ggacataact gtctaagttt
    16441 tcaatcatga ttctattttt gatcaccgtt gataaaactt ctaattgttt tggatcgtct
    16501 tcacaaatat aaatgggaat catatcatta ctcctttatt ttatttgtat ataaatcgtt
    16561 tgagaaaaac gctggttttc aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc
    16621 aattcatcca agataaacaa gccaaggcct tcgttttttg gatttgtttt tgttgagaac
    16681 cctttttgtt tcaactggtt taacggtaat gtatttgttg ttgtattgat cacacgaatc
    16741 atcacggaat gattcttctt gagtatggtc aattcgattt ttttcttctt tgcagcacta
    16801 gctccttcaa tcgcattgtc caagataata ccaagcatac gtacaaggtt taccgtatgt
    16861 ttcgtatcca gttcgattgg ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt
    16921 gccatcatga gttttaatga cagtaaacta cggatctcag gaactttcat attatccaac
    16981 atacttagat tggccggaaa aaggttcatc atctggcgtg taggtaatat cgtttgatca
    17041 taataagacc gtaatccttc taagtcgcct tctgaaatat agctgtgaag agaaaaaagc
    17101 agattttggt aatcatgttt gaattctctc aagtcttgat agtttttttc tagatctttg
    17161 atataatcct gttgcatcct gacttgttgc tctaataact gatcagctaa tttcttatta
    17221 gagattttcc aataataaac caagatgatc aggccaacac acagatatag aatgaaaaaa
    17281 ctcgtattca aagctaagac atcaggtgtt tcaccagaaa aacgggtata aaaaataatg
    17341 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
    17401 tgaaaccaag tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg
    17461 gataagacaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
    17521 acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
    17581 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
    17641 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
    17701 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
    17761 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
    17821 tacagctccc attgattttt accaaattat agcacaattg gcgtattatt tttttaattt
    17881 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
    17941 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
    18001 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
    18061 tagaaaagta agaagctgag aaaaataacg taatgagaga aaaaattaga aacatgctga
    18121 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
    18181 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
    18241 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
    18301 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
    18361 agatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
    18421 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaagaca aattttactt
    18481 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
    18541 tcacaacttt atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat
    18601 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta
    18661 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
    18721 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta
    18781 taaaaggtga tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat
    18841 atgcatttaa atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg
    18901 aaaatagtgt gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata
    18961 atctgcactg gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa
    19021 aatgttgaga gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa
    19081 tgttaaaaaa cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg
    19141 agaatactgt ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc
    19201 atgaatagga gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt
    19261 caaggctttt atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag
    19321 gatgccgata attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac
    19381 tgaaatgctt gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga
    19441 agtattgccg ctattttatt ctaatccaga aaagtatact tttttattac agattttctt
    19501 tttgaacaaa cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga
    19561 ccgctccatc tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc
    19621 tgaagtagaa gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga
    19681 aatttcgcca caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat
    19741 gttagcgaga atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact
    19801 ctattcctac tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat
    19861 atgtcccaaa atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg
    19921 agaacaaatc gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc
    19981 ccagtcgatc cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata
    20041 atataaaaac taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt
    20101 agtttttttg attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa
    20161 atgaaatgtc tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg
    20221 ggcgccaaac agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac
    20281 gacacttaga tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc
    20341 atataagttc gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg
    20401 cccgttgatt ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt
    20461 tactaatgcg ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc
    20521 ataatataga acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag
    20581 atactttgtt agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac
    20641 acttagccat tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc
    20701 agaggcaaaa gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt
    20761 cagcaaaagt cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt
    20821 ttatgaatat ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt
    20881 cagaaaacta aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa
    20941 gaaaaaatag acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa
    21001 agatgggaag gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt
    21061 gaaacgctgg gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat
    21121 cattgctacc tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca
    21181 agtaaaagaa agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa
    21241 tcgcttcaat caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat
    21301 catagcagga gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat
    21361 gacgcaaaaa caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc
    21421 aaatgaacta aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg
    21481 aacattccat agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt
    21541 agcaatgcaa ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt
    21601 tttgcaacag ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac
    21661 gattgcttct cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca
    21721 gttagttaaa gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg
    21781 actgtatgga ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt
    21841 caatgatgta ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat
    21901 cggccaaaaa tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg
    21961 ggaaatcgtg aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga
    22021 tgatcagagt atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa
    22081 agaatatcct accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt
    22141 ccaaggagca aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat
    22201 tccagcacaa aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga
    22261 aagtcagtgg ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga
    22321 atcaatcata ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa
    22381 aacgaatatt ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa
    22441 gtctttccaa catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa
    22501 gaaaaatcag gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa
    22561 aagtgaatgc gatcaatact tttctcctaa atatcaaaat atatccgttg cagattatat
    22621 tttgtcgata agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc
    22681 gttgcgtaaa acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct
    22741 gccacgtata gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa
    22801 gcagcatgct actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa
    22861 agaattaaaa acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca
    22921 gagcattcac aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga
    22981 aggaagtttg ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa
    23041 agcagaacca gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag
    23101 agcaagaaat cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt
    23161 atcgagattt atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg
    23221 gtgtgacaga agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa
    23281 atttttgtga aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg
    23341 aaaagggagg tgtgacaaga attttgtcac acctccctta tttattttga aaatgagaat
    23401 actattatca aaatagagaa tatatagaaa aattctcatt attcaaacgt aaataatcag
    23461 ttattttgat tttttttata ttttattagc taataattgg tattttaaaa gaaattactg
    23521 tagaattgga ttataattaa aaaggagtga gaaaatgata aaagtatata cttcgcctag
    23581 ctgtacttcc tgtagaaaag caaaagcatg gttgcaagcg aatcaacttg aatttgaaga
    23641 gaagaatatt tttgccgaac cgttaacaga aagtgagatc aaaaagatct tggcagcgac
    23701 tgaaggagga gttcaagata ttatttccac tcggtcgaaa atttacgaaa agctgggcat
    23761 agattttaac gaattaactt taaagcaaat gatcgtctta ttcaaagaat atccttcatt
    23821 attgagacga cctattttga ttgatgaaaa aagaatacta atcggattta atgaagatga
    23881 gatccggtca tttgtccctc gtgaaattcg atgtgttgcc agaaatctga tctatttaca
    23941 aaacaataac atcttaaatt catagggagg gagccttcgt ggaagtgaaa gtctatggat
    24001 cagcgcactg tgtaacaagt ctgaaggcga aacaatggtt agaacaccat caattgcctt
    24061 atcaatttgt tgacttagaa gaacgcgatc taagtacaga agaagcagaa gaattatgca
    24121 gttttgaaga agtgcaagca gaacagctat ttgcgacttg gtctgaggct tttcacaaca
    24181 ttgcgattga cctttcctgt gtaggaaaaa aacaactgat ttttttatgc acacaacata
    24241 ccaatctcct cagacgtcct ttgattatta tcgatgaagt tctatttatt ggatacaatg
    24301 agcagttgat ggaacagctt atcttaaata aatagacttt aactgtttta atcaagtcac
    24361 tgagcatttc atacagttgt aacagtcgaa cacttagcat caagcgtgtt atgtgttctt
    24421 tttttgactt tatttttcta ctatagagac atgctcaatt tatgtggtca aaattcaaat
    24481 ctaggtgggg atatagagtt aaaaatatat tgacatgtgt aaatttacat gatatattca
    24541 tttctgttgt tttaagtagc aaagcaataa aatagtattt taaaaaacat ataaaatagt
    24601 tgttgacatg ttagaaatga catgataaga taactgagtt gttaaaaaag aaccacttgt
    24661 gaagcttgat tctatcgaaa aaaacttcaa aaaagttctt gacaaataac aaatgataag
    24721 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
    24781 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctacggca gccaaaaacg
    24841 aatcggggaa cactcctgat gaagtagacc tttgaaaact gaacaaagta agacaaacca
    24901 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca
//
LOCUS       DADXTH010000043        24578 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.043, whole genome shotgun sequence.
ACCESSION   DADXTH010000043 DADXTH010000000
VERSION     DADXTH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.043"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(154..552)
                     /locus_tag="IWI97_001636"
     CDS             complement(154..552)
                     /locus_tag="IWI97_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBC2463135.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="IWI97_001637"
     CDS             complement(549..1322)
                     /locus_tag="IWI97_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBC2463136.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="IWI97_001638"
     CDS             complement(1319..2002)
                     /locus_tag="IWI97_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBC2463137.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="IWI97_001639"
     CDS             complement(2169..3056)
                     /locus_tag="IWI97_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2463138.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="IWI97_001640"
     CDS             complement(3053..3922)
                     /locus_tag="IWI97_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2463139.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="IWI97_001641"
     CDS             complement(4059..5345)
                     /locus_tag="IWI97_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2463140.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="IWI97_001642"
     CDS             5618..6196
                     /locus_tag="IWI97_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBC2463141.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="IWI97_001643"
     CDS             6196..7125
                     /locus_tag="IWI97_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2463142.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="IWI97_001644"
     CDS             7122..8858
                     /locus_tag="IWI97_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBC2463143.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="IWI97_001645"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="IWI97_001645"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBC2463144.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="IWI97_001646"
     CDS             complement(9754..10263)
                     /locus_tag="IWI97_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBC2463145.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="IWI97_001647"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="IWI97_001647"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBC2463146.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="IWI97_001648"
     CDS             complement(11564..12655)
                     /locus_tag="IWI97_001648"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBC2463147.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="IWI97_001649"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="IWI97_001649"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBC2463148.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="IWI97_001650"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="IWI97_001650"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBC2463149.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="IWI97_001651"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="IWI97_001651"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBC2463150.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="IWI97_001652"
     CDS             complement(16618..17592)
                     /locus_tag="IWI97_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBC2463151.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="IWI97_001653"
     CDS             complement(17573..19456)
                     /locus_tag="IWI97_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBC2463152.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="IWI97_001654"
     CDS             complement(19474..21699)
                     /locus_tag="IWI97_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBC2463153.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="IWI97_001655"
     CDS             21814..22848
                     /locus_tag="IWI97_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2463154.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="IWI97_001656"
     CDS             complement(22915..23928)
                     /locus_tag="IWI97_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2463155.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="IWI97_001657"
     CDS             complement(24006..24194)
                     /locus_tag="IWI97_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBC2463156.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7063 a         5341 c         4397 g         7777 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaactaa
//
LOCUS       DADXTH010000044        24294 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.044, whole genome shotgun sequence.
ACCESSION   DADXTH010000044 DADXTH010000000
VERSION     DADXTH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.044"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IWI97_001658"
     CDS             <1..572
                     /locus_tag="IWI97_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBC2463157.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IWI97_001659"
     CDS             576..1217
                     /locus_tag="IWI97_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2463158.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDFIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IWI97_001660"
     CDS             1214..2131
                     /locus_tag="IWI97_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2463159.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IWI97_001661"
     CDS             2128..2799
                     /locus_tag="IWI97_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2463160.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IWI97_001662"
     CDS             complement(3340..3828)
                     /locus_tag="IWI97_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBC2463161.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IWI97_001663"
     CDS             complement(4029..4295)
                     /locus_tag="IWI97_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBC2463162.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IWI97_001664"
     CDS             complement(4295..4558)
                     /locus_tag="IWI97_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBC2463163.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5034..5219
                     /locus_tag="IWI97_001665"
     CDS             5034..5219
                     /locus_tag="IWI97_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2463164.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IWI97_001666"
     CDS             complement(5361..7184)
                     /locus_tag="IWI97_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBC2463165.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IWI97_001667"
     CDS             complement(7782..10487)
                     /locus_tag="IWI97_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBC2463166.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IWI97_001668"
     CDS             complement(10793..11740)
                     /locus_tag="IWI97_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBC2463167.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IWI97_001669"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IWI97_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBC2463168.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IWI97_001670"
     CDS             complement(12375..12737)
                     /locus_tag="IWI97_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBC2463169.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IWI97_001671"
     CDS             complement(12748..13869)
                     /locus_tag="IWI97_001671"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBC2463170.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IWI97_001672"
     CDS             complement(13885..14235)
                     /locus_tag="IWI97_001672"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBC2463171.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IWI97_001673"
     CDS             complement(14365..15876)
                     /locus_tag="IWI97_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBC2463172.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IWI97_001674"
     CDS             complement(16198..17550)
                     /locus_tag="IWI97_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBC2463173.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IWI97_001675"
     CDS             complement(17703..18779)
                     /locus_tag="IWI97_001675"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBC2463174.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IWI97_001676"
     CDS             18975..20720
                     /locus_tag="IWI97_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBC2463175.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IWI97_001677"
     CDS             complement(21000..21437)
                     /locus_tag="IWI97_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBC2463176.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IWI97_001678"
     CDS             complement(21421..22794)
                     /locus_tag="IWI97_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463177.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IWI97_001679"
     CDS             complement(23116..23604)
                     /locus_tag="IWI97_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2463178.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IWI97_001680"
     CDS             complement(23626..24228)
                     /locus_tag="IWI97_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBC2463179.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6966 a         5171 c         4490 g         7667 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgattttatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgcc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctt caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttccaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc ttatgatgtg caccccaaaa gtta
//
LOCUS       DADXTH010000045        23963 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.045, whole genome shotgun sequence.
ACCESSION   DADXTH010000045 DADXTH010000000
VERSION     DADXTH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.045"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..542
                     /locus_tag="IWI97_001681"
     CDS             144..542
                     /locus_tag="IWI97_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBC2463180.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="IWI97_001682"
     CDS             526..1221
                     /locus_tag="IWI97_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBC2463181.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1815..2702)
                     /locus_tag="IWI97_001683"
     CDS             complement(1815..2702)
                     /locus_tag="IWI97_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBC2463182.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2887..3726
                     /locus_tag="IWI97_001684"
     CDS             2887..3726
                     /locus_tag="IWI97_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBC2463183.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3922..4896
                     /gene="sgrA"
                     /locus_tag="IWI97_001685"
     CDS             3922..4896
                     /gene="sgrA"
                     /locus_tag="IWI97_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBC2463184.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5194..5550
                     /locus_tag="IWI97_001686"
     CDS             5194..5550
                     /locus_tag="IWI97_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463185.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5553..6710
                     /locus_tag="IWI97_001687"
     CDS             5553..6710
                     /locus_tag="IWI97_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463186.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6736..7176)
                     /locus_tag="IWI97_001688"
     CDS             complement(6736..7176)
                     /locus_tag="IWI97_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBC2463187.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7336..7842
                     /locus_tag="IWI97_001689"
     CDS             7336..7842
                     /locus_tag="IWI97_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2463188.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8010..9923
                     /locus_tag="IWI97_001690"
     CDS             8010..9923
                     /locus_tag="IWI97_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBC2463189.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9928..10929
                     /locus_tag="IWI97_001691"
     CDS             9928..10929
                     /locus_tag="IWI97_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2463190.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11131..11337)
                     /locus_tag="IWI97_001692"
     CDS             complement(11131..11337)
                     /locus_tag="IWI97_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBC2463191.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11983..12831)
                     /locus_tag="IWI97_001693"
     CDS             complement(11983..12831)
                     /locus_tag="IWI97_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBC2463192.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12830..13897
                     /gene="gnd"
                     /locus_tag="IWI97_001694"
     CDS             12830..13897
                     /gene="gnd"
                     /locus_tag="IWI97_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBC2463193.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13899..14900
                     /locus_tag="IWI97_001695"
     CDS             13899..14900
                     /locus_tag="IWI97_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBC2463194.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14897..15181
                     /locus_tag="IWI97_001696"
     CDS             14897..15181
                     /locus_tag="IWI97_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBC2463195.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15214..16011
                     /locus_tag="IWI97_001697"
     CDS             15214..16011
                     /locus_tag="IWI97_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBC2463196.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16025..16858
                     /locus_tag="IWI97_001698"
     CDS             16025..16858
                     /locus_tag="IWI97_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2463197.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16876..17733
                     /locus_tag="IWI97_001699"
     CDS             16876..17733
                     /locus_tag="IWI97_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBC2463198.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17755..18264
                     /locus_tag="IWI97_001700"
     CDS             17755..18264
                     /locus_tag="IWI97_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2463199.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18301..18702
                     /locus_tag="IWI97_001701"
     CDS             18301..18702
                     /locus_tag="IWI97_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463200.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18814..19167
                     /locus_tag="IWI97_001702"
     CDS             18814..19167
                     /locus_tag="IWI97_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463201.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19201..19644
                     /locus_tag="IWI97_001703"
     CDS             19201..19644
                     /locus_tag="IWI97_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBC2463202.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19661..20746
                     /gene="uxuA"
                     /locus_tag="IWI97_001704"
     CDS             19661..20746
                     /gene="uxuA"
                     /locus_tag="IWI97_001704"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBC2463203.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20739..21452
                     /locus_tag="IWI97_001705"
     CDS             20739..21452
                     /locus_tag="IWI97_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBC2463204.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21659..21808)
                     /locus_tag="IWI97_001706"
     CDS             complement(21659..21808)
                     /locus_tag="IWI97_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463205.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22204..22404
                     /locus_tag="IWI97_001707"
     CDS             22204..22404
                     /locus_tag="IWI97_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2463206.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(22508..23809)
                     /locus_tag="IWI97_001708"
     CDS             complement(22508..23809)
                     /locus_tag="IWI97_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBC2463207.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         8183 a         3725 c         4947 g         7108 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
     1861 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
     1921 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
     1981 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
     2041 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
     2101 atctctggat tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt
     2161 tttccataag cttgaacaag cttggagaaa ttttctcctt catttgcttc tttttttgct
     2221 ttatctgctc cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat
     2281 tctttccaag cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat
     2341 tggaaagcca gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct
     2401 tttaattgtt ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt
     2461 acttcctttg aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt
     2521 ttttttaata gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact
     2581 gtaagtgttc cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat
     2641 agtaaaattg cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc
     2701 atcctttcct tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg
     2761 taaggttgct gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat
     2821 ttacatagtt ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga
     2881 tgaaagatga tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt
     2941 gttttaggaa ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg
     3001 attggagcaa ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt
     3061 gctagtaagg tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac
     3121 tgggggtatt tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt
     3181 ccattatatt tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg
     3241 ataacgagtt ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc
     3301 ttttttcagc atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca
     3361 ttatttgctc ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc
     3421 tttttagcag gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct
     3481 attggtgctc atattacttg gaatcggaca caaggagcta ttttgggtat accagtcagc
     3541 ggaacgcaag aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact
     3601 gggggaaact ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt
     3661 gttggtttgt tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat
     3721 caataaaaga tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga
     3781 ttaaacgaaa aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca
     3841 actgactgtc gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta
     3901 attatatgga gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg
     3961 ctgagtataa ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag
     4021 aatctttcat ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt
     4081 actattccag tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca
     4141 tatggttggg atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac
     4201 gtacaatgga ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat
     4261 gagatcaaga gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc
     4321 acggagaatt tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc
     4381 acaagtagtg aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc
     4441 agtgaaagta gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa
     4501 agtagcacag gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc
     4561 acaggaagca tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga
     4621 aaagtaaata atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat
     4681 gcagggagca acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat
     4741 catcaagaaa aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt
     4801 agaaccttga acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca
     4861 agctattttc gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa
     4921 ttgaataaaa cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc
     4981 gaagttgtag ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata
     5041 acaggaacac aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta
     5101 gtatctctga aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata
     5161 agtctaaata atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat
     5221 gaaaggcaag tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg
     5281 gtgaaggata tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa
     5341 caagcagaat cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac
     5401 aagatgataa aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt
     5461 ttagagggaa cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc
     5521 ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag
     5581 aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga
     5641 tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg
     5701 acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat
     5761 ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa
     5821 gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga
     5881 acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt
     5941 tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt
     6001 attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata
     6061 aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact
     6121 tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc
     6181 atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag
     6241 aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta
     6301 gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga
     6361 tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat
     6421 tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat
     6481 ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca
     6541 ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact
     6601 ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca
     6661 ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga
     6721 ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg
     6781 accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct
     6841 gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt
     6901 tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc
     6961 tattctgtga acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc
     7021 gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa
     7081 gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga
     7141 cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt
     7201 tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa
     7261 aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag
     7321 aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa
     7381 ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat
     7441 aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat
     7501 gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag
     7561 cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt
     7621 ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt
     7681 atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga
     7741 gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag
     7801 aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa
     7861 aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat
     7921 cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac
     7981 atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa
     8041 agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg
     8101 gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg
     8161 ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg
     8221 agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag
     8281 aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca
     8341 aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat
     8401 atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca
     8461 agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata
     8521 aagagattat caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa
     8581 acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca
     8641 caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca
     8701 aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc
     8761 atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt
     8821 ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg
     8881 attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag
     8941 ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga
     9001 tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg
     9061 gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac
     9121 taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat
     9181 ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg
     9241 ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag
     9301 atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag
     9361 atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct
     9421 acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt
     9481 atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag
     9541 cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg
     9601 ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca
     9661 aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg
     9721 gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag
     9781 tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga
     9841 aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga
     9901 tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta
     9961 ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag
    10021 ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc
    10081 cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat
    10141 ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa
    10201 ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt
    10261 cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat
    10321 gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa
    10381 gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat
    10441 tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa
    10501 aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg
    10561 tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt
    10621 tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc
    10681 aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat
    10741 ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg
    10801 ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat
    10861 cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc
    10921 aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca
    10981 aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag
    11041 gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg
    11101 tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa
    11161 agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa
    11221 gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat
    11281 agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag
    11341 cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag
    11401 tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat
    11461 aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca
    11521 ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc
    11581 caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa
    11641 taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt
    11701 aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag
    11761 aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg
    11821 aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta
    11881 ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg
    11941 ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga
    12001 aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa
    12061 aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga
    12121 tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga
    12181 agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc
    12241 aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc
    12301 cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat
    12361 aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc
    12421 gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg
    12481 ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc
    12541 catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc
    12601 attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt
    12661 tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg
    12721 actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg
    12781 gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc
    12841 tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag
    12901 agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt
    12961 gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg
    13021 aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact
    13081 cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga
    13141 gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc
    13201 tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat
    13261 tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca
    13321 gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca
    13381 tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt
    13441 caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt
    13501 cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt
    13561 ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa
    13621 tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca
    13681 tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aacggacggt
    13741 tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag
    13801 gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg
    13861 gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa
    13921 tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag
    13981 ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt
    14041 tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt
    14101 ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg
    14161 atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac
    14221 gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac
    14281 aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga
    14341 cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg
    14401 aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt
    14461 tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag
    14521 atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag
    14581 aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg
    14641 atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg
    14701 ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga
    14761 ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg
    14821 tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga
    14881 gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc
    14941 tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata
    15001 cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa
    15061 attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg
    15121 tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta
    15181 gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga
    15241 atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct
    15301 gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct
    15361 gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc
    15421 gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt
    15481 ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag
    15541 attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag
    15601 ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat
    15661 aaatacgcaa gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg
    15721 gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca
    15781 ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg
    15841 ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg
    15901 gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa
    15961 tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg
    16021 acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt
    16081 tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac
    16141 tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc
    16201 tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc
    16261 tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc
    16321 gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt
    16381 catcaatact tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc
    16441 tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct
    16501 tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga
    16561 gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat
    16621 cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta
    16681 ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg
    16741 gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac
    16801 aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt
    16861 taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc
    16921 acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac
    16981 tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa
    17041 ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac
    17101 actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa
    17161 agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac
    17221 acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc
    17281 gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc
    17341 gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca
    17401 agacaagctt tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag
    17461 acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac
    17521 tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag
    17581 gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac
    17641 ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata
    17701 ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt
    17761 gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta
    17821 tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa
    17881 tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt
    17941 acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt
    18001 ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa
    18061 gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa
    18121 tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc
    18181 gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta
    18241 ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa
    18301 atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag
    18361 cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt
    18421 atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt
    18481 tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt
    18541 catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac
    18601 tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat
    18661 aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta
    18721 gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa
    18781 acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat
    18841 gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac
    18901 agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca
    18961 gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa
    19021 gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg
    19081 cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc
    19141 aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta
    19201 atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg
    19261 gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac
    19321 atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc
    19381 atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc
    19441 aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca
    19501 atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa
    19561 caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa
    19621 aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg
    19681 gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa
    19741 tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat
    19801 tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt
    19861 agcgatccac gatgcaatca aggcagggac agatgaacga gattactata ttgataacta
    19921 tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa
    19981 accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc
    20041 tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat
    20101 acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt
    20161 cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac
    20221 ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca
    20281 tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga
    20341 cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg
    20401 atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg
    20461 catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac
    20521 ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca
    20581 agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc
    20641 tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata
    20701 tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca
    20761 tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa
    20821 aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc
    20881 aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata
    20941 tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag
    21001 tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata
    21061 caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc
    21121 tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac
    21181 cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat
    21241 ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca
    21301 tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg
    21361 taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac
    21421 gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt
    21481 atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag
    21541 tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct
    21601 accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt
    21661 atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat
    21721 tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat
    21781 attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa
    21841 aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt
    21901 ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac
    21961 aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca
    22021 atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa
    22081 tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt
    22141 gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta
    22201 tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact
    22261 ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa
    22321 actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa
    22381 gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa
    22441 gggctctttg tcaaatagga ctgatagagt gagataagaa catctaaggt agaattcctt
    22501 ttctacttta gatgtttttt gattcatagg taatcagatt atttagtaag aaaattctgg
    22561 tcttcatttt ccgaaagctt cgaaagccat aagcgactcg tttcaatgct ttgatgtggg
    22621 tatgcagatt ttctattttg gcatttgaat aaggcaattc gagggctaaa cgaatgccgg
    22681 attcatagtt tataaaagct ttttttatag aatgcagttc ttttggaacg gaattgggca
    22741 tcgttcttac aagtttaaag aaagtgtccg cctctttgtc gtgaaacgct tgaagaattt
    22801 cttgatagta aaaataggcg attcgtaatt cgtcagagat cgaaagcaga cgatccacca
    22861 cttcactttc ggtgagatac gcccaaccaa aattgcgcca tttggtccat ttgtcataaa
    22921 caagcttgtc ctgtcgtttg atcagaagcc gagacaacgc ctttaattgt cgataatatt
    22981 tggcttgtgt tggatcgttt cgatcaaagc ttttcatgat tcgtacgcga atatcctcaa
    23041 atttacgaat gagatgtttg acaatgtgaa aacgatccac aacgagtttt gcctttggaa
    23101 aacattcctt tatcagagag gcataagaaa cattcatgtc cgtcacgatg acctttacct
    23161 tcaatcgtgc gcgtcgttca aattgcataa aataagaaac gagagccttt ttcttccgtg
    23221 aaggaagaat atccagtaat tttccagtat cgccatccat acaagaaaag gagtagggac
    23281 cgacttgatt tttcagtgaa cgaaattcat ccattaataa acaacgcggt aaccaatttc
    23341 tttttacttt tgtcatgggg atcagagaag cgagtgtccg agaaacggtc gaccaagaaa
    23401 cgccgcaatg cttagcaatt gtcgtcattg cttgtttttc agttagctct tctaaaattt
    23461 tatagcgcac ttgtttagcg ataaagcagt ttctatcgac aatgtaggta ttcgccgtaa
    23521 aataggtgtg gcagccacga cagtggtagc gttgtttcgc taaagcaaga taagtcagtt
    23581 ggttcgcaca tttattaagt tgaatgagcg attttttctt tccgttctta acgatcatgc
    23641 cttcatgatt catctgttta cacaaaggac agcgacgaat ttctttactt aacgtcgcag
    23701 aaacaacaag actcttgata ttcttaatgc tcttcgattc cacatttgtg atgaaaatgt
    23761 ttttatcttt gattccaagt agtagtttga tatgatgaag atcaggcata cagaacgctc
    23821 ctttgattgt gttgtggtga ttcaattcta acggagcttc tgtatgtttt gttttttcat
    23881 gctcaaaagg gaccgataga atgattttct atcagtccta gaaattatag agccaagaaa
    23941 agacggtttt tttgtacgaa aaa
//
LOCUS       DADXTH010000046        23577 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.046, whole genome shotgun sequence.
ACCESSION   DADXTH010000046 DADXTH010000000
VERSION     DADXTH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.046"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IWI97_001709"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IWI97_001709"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBC2463208.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IWI97_001710"
     CDS             complement(1174..1869)
                     /locus_tag="IWI97_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBC2463209.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IWI97_001711"
     CDS             complement(1882..2598)
                     /locus_tag="IWI97_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBC2463210.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IWI97_001712"
     CDS             2928..3533
                     /locus_tag="IWI97_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBC2463211.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IWI97_001713"
     CDS             3858..4181
                     /locus_tag="IWI97_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBC2463212.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IWI97_001714"
     CDS             4363..5733
                     /locus_tag="IWI97_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBC2463213.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRIFQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IWI97_001715"
     CDS             complement(5863..7164)
                     /locus_tag="IWI97_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBC2463214.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9352)
                     /locus_tag="IWI97_001716"
     CDS             complement(7394..9352)
                     /locus_tag="IWI97_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBC2463215.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IWI97_001717"
     CDS             complement(9529..9795)
                     /locus_tag="IWI97_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBC2463216.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IWI97_001718"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IWI97_001718"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBC2463217.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IWI97_001719"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IWI97_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBC2463218.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IWI97_001720"
     CDS             13139..13702
                     /locus_tag="IWI97_001720"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBC2463219.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IWI97_001721"
     CDS             complement(13955..14476)
                     /locus_tag="IWI97_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBC2463220.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IWI97_001722"
     CDS             complement(14479..15321)
                     /locus_tag="IWI97_001722"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBC2463221.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IWI97_001723"
     CDS             complement(15314..16039)
                     /locus_tag="IWI97_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBC2463222.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IWI97_001724"
     CDS             16215..16511
                     /locus_tag="IWI97_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463223.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="IWI97_001725"
     CDS             17079..18671
                     /locus_tag="IWI97_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBC2463224.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="IWI97_001726"
     CDS             18684..19424
                     /locus_tag="IWI97_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBC2463225.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19592..20941
                     /locus_tag="IWI97_001727"
     CDS             19592..20941
                     /locus_tag="IWI97_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2463226.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21069..21659)
                     /locus_tag="IWI97_001728"
     CDS             complement(21069..21659)
                     /locus_tag="IWI97_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBC2463227.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21806..23158
                     /gene="brnQ"
                     /locus_tag="IWI97_001729"
     CDS             21806..23158
                     /gene="brnQ"
                     /locus_tag="IWI97_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBC2463228.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6754 a         4747 c         4490 g         7586 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatctttc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta
    19561 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    19621 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    19681 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    19741 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    19801 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    19861 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    19921 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    19981 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    20041 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    20101 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    20161 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    20221 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    20281 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    20341 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    20401 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    20461 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    20521 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    20581 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    20641 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    20701 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    20761 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    20821 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    20881 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    20941 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    21001 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag
    21061 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    21121 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    21181 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    21241 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    21301 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    21361 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    21421 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    21481 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    21541 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaaga
    21601 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    21661 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca
    21721 tcgttttata tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    21781 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    21841 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    21901 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    21961 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    22021 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    22081 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    22141 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    22201 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    22261 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    22321 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    22381 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    22441 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    22501 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    22561 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    22621 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    22681 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    22741 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    22801 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    22861 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    22921 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    22981 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    23041 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    23101 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    23161 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    23221 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    23281 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    23341 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    23401 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    23461 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    23521 tttataatat agattttaga catatatatg atataaaaat agttttcgca aaaaaac
//
LOCUS       DADXTH010000047        23453 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.047, whole genome shotgun sequence.
ACCESSION   DADXTH010000047 DADXTH010000000
VERSION     DADXTH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.047"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(16..1290)
                     /locus_tag="IWI97_001730"
     CDS             complement(16..1290)
                     /locus_tag="IWI97_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBC2463229.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            1563..4034
                     /locus_tag="IWI97_001731"
     CDS             1563..4034
                     /locus_tag="IWI97_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBC2463230.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4137..5873)
                     /locus_tag="IWI97_001732"
     CDS             complement(4137..5873)
                     /locus_tag="IWI97_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBC2463231.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5870..6574)
                     /locus_tag="IWI97_001733"
     CDS             complement(5870..6574)
                     /locus_tag="IWI97_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2463232.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6705..7208
                     /locus_tag="IWI97_001734"
     CDS             6705..7208
                     /locus_tag="IWI97_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBC2463233.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7168..7506
                     /locus_tag="IWI97_001735"
     CDS             7168..7506
                     /locus_tag="IWI97_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBC2463234.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7527..8945
                     /gene="ffh"
                     /locus_tag="IWI97_001736"
     CDS             7527..8945
                     /gene="ffh"
                     /locus_tag="IWI97_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBC2463235.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9057..9635
                     /locus_tag="IWI97_001737"
     CDS             9057..9635
                     /locus_tag="IWI97_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBC2463236.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9639..10160
                     /locus_tag="IWI97_001738"
     CDS             9639..10160
                     /locus_tag="IWI97_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBC2463237.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10239..10793)
                     /locus_tag="IWI97_001739"
     CDS             complement(10239..10793)
                     /locus_tag="IWI97_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBC2463238.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11046..11321
                     /gene="rpsP"
                     /locus_tag="IWI97_001740"
     CDS             11046..11321
                     /gene="rpsP"
                     /locus_tag="IWI97_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBC2463239.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11333..11584
                     /locus_tag="IWI97_001741"
     CDS             11333..11584
                     /locus_tag="IWI97_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBC2463240.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11709..12500
                     /locus_tag="IWI97_001742"
     CDS             11709..12500
                     /locus_tag="IWI97_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463241.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12670..13191
                     /gene="rimM"
                     /locus_tag="IWI97_001743"
     CDS             12670..13191
                     /gene="rimM"
                     /locus_tag="IWI97_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBC2463242.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13181..13942
                     /gene="trmD"
                     /locus_tag="IWI97_001744"
     CDS             13181..13942
                     /gene="trmD"
                     /locus_tag="IWI97_001744"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBC2463243.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14078..14425
                     /gene="rplS"
                     /locus_tag="IWI97_001745"
     CDS             14078..14425
                     /gene="rplS"
                     /locus_tag="IWI97_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBC2463244.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14683..14976
                     /locus_tag="IWI97_001746"
     CDS             14683..14976
                     /locus_tag="IWI97_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBC2463245.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            15819..18146
                     /locus_tag="IWI97_001747"
     CDS             15819..18146
                     /locus_tag="IWI97_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2463246.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            18272..18601
                     /locus_tag="IWI97_001748"
     CDS             18272..18601
                     /locus_tag="IWI97_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBC2463247.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            18641..19645
                     /locus_tag="IWI97_001749"
     CDS             18641..19645
                     /locus_tag="IWI97_001749"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBC2463248.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            19642..21071
                     /locus_tag="IWI97_001750"
                     /pseudo
     CDS             19642..21071
                     /locus_tag="IWI97_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            21277..22449
                     /locus_tag="IWI97_001751"
     CDS             21277..22449
                     /locus_tag="IWI97_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2463249.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(22611..23066)
                     /locus_tag="IWI97_001752"
     CDS             complement(22611..23066)
                     /locus_tag="IWI97_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463250.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         7827 a         4292 c         4726 g         6608 t
ORIGIN      
        1 gcgccgcata tttttttagt tttcaaagat cagaccttgt tgtaaataga tccgtgcccg
       61 aaagttataa aagttgcgat aaccaaacgc aattcgttta atcagcttga tcttgttatt
      121 cagcccctca gtgacgccgt ttgagtggga caccttaaag gcatttgaaa tacccgattg
      181 gtagcaatgg aagatcgtga atttcttttt aaacgactga ggtaacgtag atgaaattgt
      241 tttaagggtt tcttgaaagc cttccgtacc tcgtttaacg aaataatagc gtaataattg
      301 acaaaaatga tacgcagttc gcaattcttc gttgtagctt aacagctcat caacgatttc
      361 tgtttgacta attggtcgtt taaacagtcg atgataatgc cgtttgagtg gctcccatgt
      421 cgtggaatct ttgagtaaga gtttccaata tcgcttgagc cgacggtact tcttctgatt
      481 ttcagagtgt gaggttcgaa aacgattcat gatctggaca cgtaattgat tgaagctgcg
      541 attgatgtgt tggacaatat gaaagcggtc agttacgatc tccgcatttg gaaagactgt
      601 tttaagcaac tgacaatagc tggcattcat gtccatcact aggtacttga cctttaaacg
      661 ggcctttcta ggaaagcgca ggaagtattg agttagtttg taaagtctgc gatcttcaag
      721 gatcgtaaat agctgattgg ttgtcccatc aacacaaata aaactcatgg aaccagaaac
      781 agaattcatt gatttgaact catccataca caacaccgtc ggaaggaagt tcatacgtgg
      841 ctggtaatgc ttcgttgctt cacgcaagat ccgtgtcacc gtcccatccg agacgaagta
      901 cttcttagcg attagtttac gtgattgatt ttcgtacagt tccatgagaa tcgcctgctt
      961 cagttccttt gttagttgat gatgttcatc aactaagtcc gttttcgcag aaaaggtcct
     1021 tccacaatca tggcagagaa aacgggtacg ttttaattct aaaatagtta acttttcttt
     1081 gactttcaac atctgagtgc gtgtcgtata ggaaccgttg cggataatac gaaccgatcg
     1141 acagtgttca caataggaag aagagtcatc caattttcct ttaataaact gcgcttgaac
     1201 gcctttatat tctttttcaa tcagccattc ttcatcaaaa aataaatgtt tatctgttaa
     1261 tccgagtaat tttctagtat gattatccat tgagagactc ctttggtagg atgacgtttt
     1321 ggtcgactaa attttaccaa aagtgagtct ctttttgagt tgaaacgaag aaaaagtgtt
     1381 agtagaaaat cgccttcgat tttccaccaa cacaaaatat tatagaaccc tttttttttg
     1441 ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt tccatatctg
     1501 ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc
     1561 acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa agatcatcag
     1621 ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat caagtcaagg
     1681 aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa acaaatcagc
     1741 aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc ttagccattt
     1801 tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc atagccaata
     1861 caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt
     1921 taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata gatatagaag
     1981 accttgtaat agatgacatt gttattttag agactggata tcaaattcca gcagatgcag
     2041 aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa gcagatgaaa
     2101 taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc ggaaaagctt
     2161 acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca gtcaaagcca
     2221 agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat
     2281 ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt tactttttta
     2341 atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc ggtatgatac
     2401 ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact aaactagcaa
     2461 agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg gtaaatgtcc
     2521 tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa aaaatgatcg
     2581 tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc attgctgact
     2641 atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca tattttacaa
     2701 aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa aaattcagca
     2761 gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc
     2821 aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg
     2881 tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca gtcacaccat
     2941 taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca tttgattact
     3001 tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc accgtatcca
     3061 atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca tctaaattat
     3121 cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc gtcaagccag
     3181 aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct atgacaggtg
     3241 atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg atggcttccg
     3301 gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac ttttcacgca
     3361 tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga tcagccagct
     3421 tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg atctttgctc
     3481 tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact atcggacttc
     3541 catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga
     3601 atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc ggtgcgttgg
     3661 tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca tctaccatgc
     3721 ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg aataaattca
     3781 gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc tttttgcata
     3841 agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc attctatttt
     3901 tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg tttgaacgta
     3961 aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta tcgatattta
     4021 aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta
     4081 tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac
     4141 attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc
     4201 gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct
     4261 gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc
     4321 tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga
     4381 tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc aatcagattt
     4441 ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat
     4501 cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa
     4561 taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg
     4621 atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa
     4681 gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc
     4741 agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc
     4801 ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct
     4861 tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg
     4921 atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt
     4981 ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt
     5041 agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg
     5101 ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga
     5161 tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga
     5221 tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg
     5281 ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca
     5341 agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc
     5401 ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt
     5461 ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg
     5521 ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca
     5581 ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc
     5641 cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg
     5701 ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc
     5761 tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa
     5821 ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg
     5881 gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat
     5941 ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg
     6001 cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc
     6061 gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt
     6121 ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag
     6181 gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac
     6241 gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta
     6301 ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat
     6361 ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat
     6421 ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg
     6481 tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag
     6541 aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat
     6601 tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg
     6661 tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg
     6721 catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga
     6781 tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac
     6841 caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc
     6901 aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga
     6961 tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa
     7021 gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg
     7081 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
     7141 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
     7201 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
     7261 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
     7321 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat
     7381 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
     7441 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
     7501 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
     7561 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
     7621 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
     7681 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
     7741 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
     7801 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
     7861 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
     7921 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
     7981 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
     8041 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
     8101 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
     8161 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
     8221 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
     8281 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
     8341 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
     8401 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
     8461 acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
     8521 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
     8581 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
     8641 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
     8701 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
     8761 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
     8821 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
     8881 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
     8941 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
     9001 atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga
     9061 gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg
     9121 ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg
     9181 gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat
     9241 tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca
     9301 aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct
     9361 tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa
     9421 aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag
     9481 atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct
     9541 atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag
     9601 aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg
     9661 gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg
     9721 acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc gatacgacca
     9781 tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc
     9841 cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg
     9901 tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata
     9961 tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg
    10021 aatggtatat ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag
    10081 gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat
    10141 tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt
    10201 cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa
    10261 aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta
    10321 ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt
    10381 ggcacattca aaaagacaag tgattgtcgt agatggtggt ttgcgccaaa gccgccgatc
    10441 ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa
    10501 ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca
    10561 ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca
    10621 gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt
    10681 acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg
    10741 ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt
    10801 ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga gcaaataaaa
    10861 acacttactg acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc
    10921 taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt
    10981 tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat
    11041 aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc
    11101 gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta
    11161 cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact
    11221 ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg
    11281 ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga
    11341 tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa
    11401 gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga
    11461 tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta
    11521 cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa
    11581 gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt tttattaatt
    11641 gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg
    11701 aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta
    11761 gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta
    11821 ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca atggctacct
    11881 caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca
    11941 tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc
    12001 aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat
    12061 tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag
    12121 aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa gtggacttag
    12181 gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag
    12241 aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat cccttactat
    12301 tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg
    12361 gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag
    12421 aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag
    12481 tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt
    12541 cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta
    12601 aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg
    12661 ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa
    12721 ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg
    12781 gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc
    12841 aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa
    12901 aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat
    12961 gaattctatt accatgagat catcggtctg catgtattag aagatggaac tgagttaggt
    13021 aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa
    13081 aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct
    13141 ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac
    13201 tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga
    13261 taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca
    13321 gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat
    13381 ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct
    13441 tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga
    13501 acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt
    13561 gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt
    13621 catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca
    13681 aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata
    13741 taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg
    13801 gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact
    13861 tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa
    13921 aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg ttgataatat
    13981 gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg ctgtgagaaa
    14041 agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt
    14101 aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt
    14161 tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat
    14221 caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt
    14281 gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg
    14341 ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc
    14401 tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa cccttggtat
    14461 tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt tttctttaat
    14521 tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta gtaaaaaaaa
    14581 gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc cagaatattc
    14641 taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta tacttatacg
    14701 gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt aaaacaagta
    14761 ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg ctttgaccat
    14821 ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt ttctagtggc
    14881 gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag aagttcagac
    14941 ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat actcattgaa
    15001 aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt ttgtcatctg
    15061 tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga taaaagctaa
    15121 tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag cgtacatgaa
    15181 aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt acgaataaat
    15241 accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa gataacaaga
    15301 tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa acagtatttt
    15361 gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac cattttttaa
    15421 gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca caacactgca
    15481 gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa gaaaaatagt
    15541 agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt aagcaatact
    15601 tcaattaatc aagttttatc tttttttttc aataatcgta aaaaaagagt attttttatt
    15661 taaaaataaa atctttgtat taaaggacaa gactaaaata accttagaat aaaaagagaa
    15721 aaaaagcccc aaactttcta tacatcaata aaataataaa aattaaattt agaataacgt
    15781 gtaaaggatt atttgatgat tttttattga gaggagggat gaaaatgatt aagaaaactc
    15841 aaaataaaat ccaacgtata tgtcaaaaac attttgtttc tagactcaaa gctgtatcta
    15901 aaagaaaata cttacatccc attatgattt cgatcatctt gatttttact ttcttaggga
    15961 gcttatcaag tatttatgta aacgtttata cagaaacgtc aaattttgct aaccaaacga
    16021 cagaggagac aaatagtcca ccctattctg aaatacagtc catagaaagc accaatcagt
    16081 ctagcaacaa cgagacaacc tcaaccacat cctcgactca actgattgat ccgacagaaa
    16141 aagaaatcac agcagcaaaa gcagcagggg agaaagctta ttcagaaaca ggtagaagcc
    16201 agacatttag tactgttgcg gccactgcga atttcaacgg acataccata ccagatggat
    16261 atacagtgat atatgccaaa gatggagtca ttgttgcggt taacggatcg atgagtgcgg
    16321 ataaaactgc gttacaaggc gtaaccaaag caatcgcttt tattgataac cgtccagata
    16381 tccataatct gtttattggc tccacagtaa atggtactac tatcacattc ccaaaaagta
    16441 tctatatggg ggtggctacc gctggaaata cgatcgattc agttacaccc aatgcggata
    16501 aaagttattc ttttacaccc tcaacatggg gagggcttcc tgccggagga ggtatgagta
    16561 ctttttcaat cggtatacag ttagcaaatg atattccatt cacggggtac tcgatcaaaa
    16621 atgggcagat cgttgatggt aagggaaatg tttcaaattt tccaacctat caagcagcaa
    16681 cggttcaggt caatgcgaat cggacaggaa ctttttctta tacagtcaat ggtggagcag
    16741 caaaaatcgg aacgtacggg actacattat ctggaatcca tgtgggtgac aaagtggtta
    16801 tcactccaac agcagtaacg ggctattctt ttgcacaaac aaacggttct ttcacagccc
    16861 tagaaagtaa cgtcgatacg gtgacctata caggcaaaca aacaccaatc actattaagt
    16921 atcaagacta ttttggacag atgattgctc cttctaaaat aatgaatctt acttacggaa
    16981 gtgctgcaca agacctgacg actaatacgc ctactatcaa cggatatacc tttacagctg
    17041 tttcagcgac agaaacaaag aaccaatcag caatgagtgt tttagctagt ttagatacaa
    17101 atggaaatgt aatcgtaaag gacgcaaatg gaaaacagat ttctgaagtc atcctatatt
    17161 ataagacaaa tgcaacagta tcaatcaatg caaatggcag taagtactat gatggtcttt
    17221 ctgttgtacc gatcgttaaa tatgccttta atgataaaac agaatcaaca tcccttttaa
    17281 acaatgatct cagtattgat caaatcacat ggaatccaac tgatttcaaa gcgatgaatg
    17341 aaaagggagt tgaagtaact gcgcctactg aaattggaac ctatacgtct tggcaattga
    17401 caaaaagcgg actagctaaa ctagctgcca gaacaaacta tttatttact atcgtgcaga
    17461 catctgatgt ctatacgatt aagcaaatca gcggtgcggt agagctcagt ggcagtaaga
    17521 catatgatgg aaaagcaggt gtcccgtcaa tccatgtcaa gctagcagag ggtgtcacta
    17581 gttcgcttac tccagtacaa gtggcactta gcagtatgga ttacacagca gatgcacaat
    17641 cagcaaaaaa tatggttaat gctggcagct atacaattaa actaacgaat agcggaatag
    17701 acaaagtcaa agcagcaaat cccaagttgt catttactga tatagccaac acatcaggaa
    17761 cttataccat ctataaagct aacgcagtga ttactgtcga tgacgctgcc tttaattatg
    17821 atgcccaaag tcactcgatt tcggttggaa atctccacgt taccggtgtt gtcttaggcg
    17881 aatcattgga ttatacccta acagttaaca gccggacaga tattggatca caaatagtaa
    17941 cgattagttt aggcgaagga agtgtaaaca ataattatac gattatcgca acagataccg
    18001 ctaagttgac gatcaatccg actctcttgc ttccttcgac aggtgggata ggtctactgc
    18061 cctttgtctt tttaggactg atctttattt tttctggctt cttttatttt atccatcgta
    18121 agaaaaaagc aggtgaacag cgatgaaaaa aacaaaaaat gtacgtgtgg atatatttgc
    18181 tgttctcttt tttatcctac tggtcttttt tataggttct ccgactgtgt cagctagaga
    18241 atcacctatt acatttactt tgcataatcg aatgttcgaa agcgataaga tgccaagtat
    18301 cagtcaaaac gacggaataa atatcccgga taactttcaa ggagtaaaag ggttgaacgg
    18361 cgttactttc gctatatatg atgtctcgga tgaattttac aagctgcggt cagaaggaag
    18421 tagtgtggaa gacgcacaaa gaaaactagc acaaaaaagt gatagtgaaa aaatactggc
    18481 agaaaatgtg acaaaaacag tagacgagga agagggcatt gcttctttta gtgcatcgga
    18541 caaagatgag caaggtcgtg acgctgttta ccgatttgcg gaaataaaga cttcagatta
    18601 agtaaaagaa aaatcagcac catttgtcgt tgtgcttcct gtgacagatt catctaatca
    18661 taaattgacg accatccact tgtatccaaa gagcgaacaa aaaatacctg ctgcactcac
    18721 attaacaaaa acagtagaga ataagcagac agattttgca gatggggata aagttcctta
    18781 tctcatcacc gtaacaattc ctgagaacat caacgagata ggaacctata cgatcaaaga
    18841 tacagctgat cctcagttat ggctagaacc agaaacgatc gattttttga ttggtggaga
    18901 caagattcat acatttcaca cacaaaaaac aaaacatggt tttgtcttat cagattctgg
    18961 aaaagatctt agttcgtttg ctgggaaaaa attaacgatc cgttaccaaa tgacattgaa
    19021 agaaaacagt gaaaaaacga catttgataa cgatgtacgt ttgacgactg acaatcaaac
    19081 agtagaaaca catcttgcca tccaaacagg aggcaaacaa tttgtcaaag tggatcggca
    19141 aacgaaacaa aaactagcag atgctcaatt tctagtcaaa aaaaatgacc aatacttagc
    19201 gcaagaaaac gggataaatc attgggtagc caaagatgat tcaagagcaa cgatttttac
    19261 ttccagtaaa gatggaacat tttctgtcca tggattgtct tttggaagtt acgaactagt
    19321 ggagattaaa ccgcctaaag gatatatatt gagtcaatca accattctct ttgaagttga
    19381 aaaaggcagt tatgatccgc ttcatcctat accattggaa atcatcaacg aaccaaaagc
    19441 aaatccgacg aataatggaa aaacgccagt tcagatgcag acaaatggtc catcaaatga
    19501 aaaaaatgga ggaagctttc caaaaacaag cgaagagatg cttggaggtt tctcaatttt
    19561 agggttgttg ctagtccttt caacagggac agcttggttt tataaaaaac aacaaaaagg
    19621 aaacaatagg agggaaataa aatgaaaaac atcaaaacaa aaatatggac gtttttagga
    19681 actgctatca tgctgcttcc tttcgttctc ggcctaggaa ctgcagaggt ttctaggccg
    19741 tatcacctac accagaaaat gttacggtta atttgcataa attgaaattc acttcagcac
    19801 cagaaaatca aataaacaac ggaacggaat tgacatttcc gaatagcgaa ccgttgaacg
    19861 gcgttgaatt caacgtatat gacatcactg ctacgtacta tccaagtaaa gacacagcag
    19921 ttccagcaga cgctacaccc ttcgcaagtg taacgacttc tggtgaagga ttagctaacc
    19981 tgactcttcc aggtaaatct gatgggaaag atgctgtata cgtctttgta gaaacaccaa
    20041 agcccggtgt cgaaacgtcg ccaaatatcg tgttaagctt acccttttat aacccgaatt
    20101 catcagaccc tgacaaacct ttagacacga ttcacctata tgctaaaaat gtcatcaaag
    20161 aaagcaatat caaaatccag aaagctggga acttttcagg ggtagataag ttagcagggg
    20221 cgcaattcca gatcaaaaat tcagagaatc aattcgtgac cggttttgat gaaaacactg
    20281 gtgtcgctct ctatgatcct gctgagtcag cgttgacatt cacaactgat actgaaggat
    20341 ccatcagtgt caatggatta atagacggca attatacttt agttgaaaca aaagcaccag
    20401 atggttatca attaaccaat ccacaaaaag aaactacttt tatagtaaaa aacggaaaat
    20461 tgcaacaaac aaccggtatc atccatggcg cagatgaagc caatcttggg aatccaaacg
    20521 gtgtagtcaa tcgggaaaat acaactacgg ataacacgat tactgtagaa aatgtccaaa
    20581 actacggtga tttccacttc atcaaacaag atgtgaacac gcaagaaaaa ttagccgatg
    20641 cagaattttc tattctaaga gcaaaagatc caactgttaa attaatgaaa aaaatagcaa
    20701 atcttgcctc aggagaatac ccatatgctt ggtcagatga agtcacaggt tctgactggg
    20761 aagaagtgaa attgatttct gattcagaag ggaaattttc aatcgaaggg ctgaaacaag
    20821 gggcatactg ccttcaagaa acaaaagcac cagaaggcta cgcattacct aagaatgaag
    20881 ctgcgtacac tgaatttaca gtagatgatg atccaacaac ttctgatgaa acaacgatcg
    20941 ataaccaacc taaaggaatc ttgccttcaa ctggtggtat ggggatcatc gtattcatct
    21001 tggtaggtac tgctttagtt ggaggagcag tgatctactt caaaaaacgc catcaagaaa
    21061 cagaagctta aactattgga aacaaaaatc atacgataaa taaacgatag tgattcaaat
    21121 ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt cgctcaacaa acggcaaaat
    21181 aggaggaact attttgccgt ttgttgagcg aatactcagg tcaaggcgct gatttcacaa
    21241 cctcttcata tacatattag aaaataggtg gacatgatga ataaaagcag aataagaata
    21301 ctggacatct tcatggcaat cattttagtc gttgggatcg ggatcttttc ttatcctttt
    21361 gtagaagatt cactgaatga ttttcttgcc cagcaaatga tcatccatta tcaaaagcaa
    21421 gcatcgaata agaattcaga ggaaataaaa aaacagcagg aaaaaatgac aaagaaaaac
    21481 caacagttgg cagagcaaaa cgtatcacca gggataggca gtttcaacca agcagttgat
    21541 gcaaaagcat taaaagattt acctagtaat gcctttttta tggaacatat gcttggagtc
    21601 atcgaaatcc caaaaatcaa tgtcagctta cctatattcg atcagacaac ggaaatattt
    21661 ttgcaaaaag gaacgtcatt attggaagaa agttcttatc cgactggcgg gaaaaacacc
    21721 catgccgttc tttctgggca tcgtggactc ccagaagcaa aattatttac agatttgcct
    21781 aagttgaaaa aaggagacca gtttttcatc cagatcaatg gaaagacatt ggcgtatcaa
    21841 gtagaaaaaa tccaagtggt cttacctgat gaagtagatt ctttgggtat ccaaaaaggc
    21901 agagatctcg tgactttgct tacctgtact ccttatatgg tcaatacaca tcgactgctt
    21961 gtgacggggc atcgtattcc ttatcaagca aaagaggcaa aaaaagcgat ccaaggaatt
    22021 gatcaatgga aaaaatggaa attttttatt tggtttatcg gtatactact tggatcaatc
    22081 ggcttagttt ggctactcat cgcttattta gattctttgg ccatcgctaa gcgaaactat
    22141 cctttatctt tttatgtaaa gaacacaaac ggacgaccaa tcgaaggaat ggtcttttct
    22201 gtaaaaacgc tcaacggcaa acactatatt actagagaaa aagttccatt tgtgaaagct
    22261 tctgatgaat acggactagt gagattttct gatttaaaag gcaggaacta tcgcttgcaa
    22321 catgaggagc tcctgcttaa aatccatgta aaacataaac attcgaaaca gttttcaatg
    22381 aaattgaaaa aaggaagata taaactgcga aaagaaaaag aggtgtacta tttgattgag
    22441 aaagaatgaa tgaagatgtc caaaaaagca gagggaacag actaaatgaa aaaagagaga
    22501 agctgacgaa atggacgtgt aaactgctat ttttggttta ctatctggaa cgccagcttc
    22561 tattttttta tgtttcagaa gaattttatt attatagtat aacttttgaa ttagtttcca
    22621 taacatttgg agcaaggggt taaaccacga gcttgagctt cgcttaaagt ggcaggggta
    22681 tacgtaccat ttccacattt atgtgtatgg tattttgaac cggtcggtgt gacatagaca
    22741 gtttgttcat tattttgctc aggagctgcg gcttgtgcct gctgagaggc ctgttcagct
    22801 gctgcttgct gtcgcgcctg ttctgcttgt tgctgtgcga ttgcttcttg ttgcgcttgt
    22861 tctgcttgtt gtcgtgcaat ttcttcttgc tttttccgtt cttcttctgc ttcttttgct
    22921 ttgattgtgg aatcaattgc agcaagacgg cttgtcaact cttgtttgtt ttcaggtagc
    22981 gcttggatag ctgctgaagc agttgcatag ttttcttttg taggatttgc ttcagcttgt
    23041 gacaaagcag attccgcttc tgtcactttt ttttctagtg cagctttttc cgctttttgt
    23101 ttctcttctt cttttttctt ttcaatttgt tcttgttctt tcgatgaatc tgccgtactt
    23161 tcggagatcg tggtactgct gctggaagcc tgagctgaac ttgtgctttg agtagtagac
    23221 gtagtagcac cacacccact caacaaactt cctgcgacca aaaacaatgc aactaatttt
    23281 ttcactaaat ttcctcctta agtaaaaaac aaccgttttc aataaatgtc cttttcaatt
    23341 gtttacttac ttgttataat aattgtgtta tgaatagtga ttgaggaaac ttaatctaat
    23401 cccgttttga tacacgggat tttttgctat agttctgtta ttaagtatgc aat
//
LOCUS       DADXTH010000048        22684 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.048, whole genome shotgun sequence.
ACCESSION   DADXTH010000048 DADXTH010000000
VERSION     DADXTH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.048"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..3659
                     /locus_tag="IWI97_001753"
     CDS             132..3659
                     /locus_tag="IWI97_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBC2463251.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="IWI97_001754"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="IWI97_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBC2463252.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="IWI97_001755"
     CDS             7447..7788
                     /locus_tag="IWI97_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2463253.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="IWI97_001756"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="IWI97_001756"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBC2463254.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="IWI97_001757"
     CDS             9145..11565
                     /locus_tag="IWI97_001757"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBC2463255.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IWI97_001758"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IWI97_001758"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBC2463256.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="IWI97_001759"
     CDS             12999..13736
                     /locus_tag="IWI97_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBC2463257.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="IWI97_001760"
     CDS             13750..14565
                     /locus_tag="IWI97_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBC2463258.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="IWI97_001761"
     CDS             14578..15225
                     /locus_tag="IWI97_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBC2463259.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="IWI97_001762"
     CDS             15241..15897
                     /locus_tag="IWI97_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBC2463260.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="IWI97_001763"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="IWI97_001763"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBC2463261.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="IWI97_001764"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="IWI97_001764"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBC2463262.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="IWI97_001765"
     CDS             18239..18757
                     /locus_tag="IWI97_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBC2463263.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="IWI97_001766"
     CDS             18864..19364
                     /locus_tag="IWI97_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBC2463264.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19644..20564)
                     /locus_tag="IWI97_001767"
     CDS             complement(19644..20564)
                     /locus_tag="IWI97_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBC2463265.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20729..21742
                     /locus_tag="IWI97_001768"
     CDS             20729..21742
                     /locus_tag="IWI97_001768"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBC2463266.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="IWI97_001769"
     CDS             complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="IWI97_001769"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBC2463267.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7562 a         3953 c         4827 g         6342 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga tttaaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagcgttt aactccaaga aataagaagg aattcacgaa aattactttt
    19501 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    19561 cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag
    19621 attcgttgca tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg
    19681 aaaccagaag ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat
    19741 tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc
    19801 actaatccaa agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta
    19861 cgatttactc gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga
    19921 atatctaaaa gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga
    19981 ttcgggatga aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca
    20041 gatacgacgg ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc
    20101 tgttgttcca tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca
    20161 agaccgattg ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg
    20221 atagcagata aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt
    20281 aaaagactat gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga
    20341 ttaaaagact tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc
    20401 aaaaaaatca gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc
    20461 ccgtcccaaa accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg
    20521 ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta
    20581 aaatttgatt ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa
    20641 ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg
    20701 tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc
    20761 ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg
    20821 agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg
    20881 tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt
    20941 tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat
    21001 ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt
    21061 tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg
    21121 tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt
    21181 aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg
    21241 tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact
    21301 tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac
    21361 acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa
    21421 attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca
    21481 gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct
    21541 agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg
    21601 gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac
    21661 aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg
    21721 ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa
    21781 aatcaggaag agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat
    21841 gcaacaaaag tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt
    21901 ccgctggaaa gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca
    21961 ccttgctgat caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca
    22021 cctgtgacag ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta
    22081 ggcgagaact cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc
    22141 atcatgttat aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg
    22201 ctgtttggtg caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg
    22261 atatcttgag ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct
    22321 tctttgttgg cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg
    22381 atggcattca ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat
    22441 gcttcttcgc cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag
    22501 ataatttctg ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg
    22561 ttagcgacgc ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt
    22621 tgtttctgat aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc
    22681 ttga
//
LOCUS       DADXTH010000049        22635 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.049, whole genome shotgun sequence.
ACCESSION   DADXTH010000049 DADXTH010000000
VERSION     DADXTH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.049"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1111
                     /locus_tag="IWI97_001770"
     CDS             <1..1111
                     /locus_tag="IWI97_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HBC2463268.1"
                     /translation="IFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASILVLLYF
                     LKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQFTFMKI
                     MSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKDRVGLA
                     KLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFLGLYML
                     VSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSCTLILK
                     RIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLLILVVA
                     GIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            1160..1879
                     /locus_tag="IWI97_001771"
     CDS             1160..1879
                     /locus_tag="IWI97_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBC2463269.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            2478..3425
                     /locus_tag="IWI97_001772"
     CDS             2478..3425
                     /locus_tag="IWI97_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2463270.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            3437..4357
                     /locus_tag="IWI97_001773"
     CDS             3437..4357
                     /locus_tag="IWI97_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2463271.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            4398..5855
                     /locus_tag="IWI97_001774"
     CDS             4398..5855
                     /locus_tag="IWI97_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2463272.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            6200..6814
                     /locus_tag="IWI97_001775"
     CDS             6200..6814
                     /locus_tag="IWI97_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBC2463273.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            6807..8531
                     /locus_tag="IWI97_001776"
     CDS             6807..8531
                     /locus_tag="IWI97_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBC2463274.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            8528..9979
                     /locus_tag="IWI97_001777"
     CDS             8528..9979
                     /locus_tag="IWI97_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2463275.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(10452..12605)
                     /locus_tag="IWI97_001778"
     CDS             complement(10452..12605)
                     /locus_tag="IWI97_001778"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBC2463276.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(12607..13653)
                     /locus_tag="IWI97_001779"
     CDS             complement(12607..13653)
                     /locus_tag="IWI97_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2463277.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13803..15089
                     /locus_tag="IWI97_001780"
     CDS             13803..15089
                     /locus_tag="IWI97_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBC2463278.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVE"
     gene            15105..17807
                     /locus_tag="IWI97_001781"
     CDS             15105..17807
                     /locus_tag="IWI97_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBC2463279.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            17792..18649
                     /locus_tag="IWI97_001782"
     CDS             17792..18649
                     /locus_tag="IWI97_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2463280.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(18720..19619)
                     /locus_tag="IWI97_001783"
     CDS             complement(18720..19619)
                     /locus_tag="IWI97_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBC2463281.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            19788..21959
                     /locus_tag="IWI97_001784"
     CDS             19788..21959
                     /locus_tag="IWI97_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBC2463282.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            22027..22368
                     /locus_tag="IWI97_001785"
     CDS             22027..22368
                     /locus_tag="IWI97_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBC2463283.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT         7483 a         3997 c         4675 g         6480 t
ORIGIN      
        1 tattttttat atgctattgg ctacattcat catcatgaaa gtcatggatg gtgactatgt
       61 gacggcggtc acgcaatcaa ctttcgcggc atttatcggt atgctagcaa gtattcttgt
      121 ccttttgtat tttttgaaga aacatcaagc gtatacatcg gcgtttattc actacagcga
      181 aaacaaaata aatattgcaa cgaaggaatt gttgttagat accgttaaag aagcgattcc
      241 gtttattatt gtgggttcag gggtaacgat cttcaaatta gtcgatcagt tcacttttat
      301 gaagatcatg tctgcatcca ccgagtattc caatgcacag ttactggatc ttttttcgat
      361 ttttagtgcc aatccagata agttgacgat ggtagttatt gcattggcaa catcgatcgc
      421 gtctactggg ttaccgttga ttacagaagc tgttacgttg aaagatcgag taggtctggc
      481 aaaattgaca agcggaaatt tacagttgtt ttcatttttt atgtttccgg cgacatttgg
      541 tgttatgtta cttgcctatc cgctaaatac attattttat acaccggatt ctctaggaag
      601 taatgtactg atccaagcaa gttttgtagg actcttccta ggactatata tgctagtttc
      661 caacatgctg caaggaatgt ttgaaaacaa ggcggcaatt aattatttgg ttattgggtt
      721 tctagtgaaa ctccttcttc aatacccagc cattcgttta tttgaagtat acggcccttt
      781 attggcgaca atgatcggat ttgctgtgtc ttgtaccttg atcttaaaga gaatccatga
      841 agtagcccgc tttaaaccaa gttttgtctg gcgcaggaca ttgctgatct ttattcttac
      901 cttgattatg cttttggctt cgtggatcac aaagatgatt tttggtgctt ttttaaacga
      961 agatagcaaa ttccagtctc tattgttgat tcttgtagta gcaggtatag gggctttagt
     1021 gtatggttat ttagctttga aaatccgctt ggcagaaaga ctactaggac caggaatagc
     1081 gcgtttacga cataaattaa aaataaaata agtgaatcag aagagctaga ttgatgctct
     1141 tttgaaatgg gggaaacgca tgcgtttaga taagttttta gcagatatgg gacttggcag
     1201 ccgcaaagaa gtcaaaggat taatcaaaaa aggaacgatc gaagtcaatg gacaagtggt
     1261 gaaaagcgat aaagcgcaag taaatgaatt cgaagatgcg gttacttatt taggagaaat
     1321 cattacgtat caaaaagatt tttactacat tttgcataaa cctgcaggag tcatttctgc
     1381 tactcaggat aatcatgatc aaaccgtaat cgatttattg gaagaccagg attatcggga
     1441 agatcttttt ccagtcggcc gattagataa agacacagag ggcctcttga ttttaacgaa
     1501 tgacggggtg tttgcccatc agttactatc acctaaaaaa catgtggaga aagaatatct
     1561 agctgaggtt agcggtataa tgacagaaga agatatccgt ttatttgcag aaggactgat
     1621 tattgatggc gatgagcaga cattgccggc aaaactccaa attgatcaag tagatcaaga
     1681 gacagaaact tcaattatcc gtctgatttt gcatgaagga aagtttcacc aagttaagcg
     1741 aatggtcaaa gcagtaggaa aagaagtctt atatttaaaa agaatccgta tgggaaaatt
     1801 ttggctgcct aaagatttga ataaagggca atatcgaccc atgaccaaag aagagctaga
     1861 acaagtgaga gaaaaataat tgaatggata gctaaaagaa taagcagagt agttgtagcg
     1921 ccatctgaaa agcagacttg tccgtctgac tttcggatgg cttttttgtg aaatttcggc
     1981 ttatttccgt agaagctact tctggaacac cgtttgttcg gaataaggga ctgtgacaag
     2041 acttttgtta cagtccccat tgttccttgt gactcagagt tagatagtaa ggacacgatc
     2101 tccctaatac tcaggccaaa acgctgaatt cacaacctct gataaacggt gttcaatcag
     2161 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt cccgtttttt
     2221 tctctaatac tcaggccaaa acgctgattt cacaacctct gataaacggt gttcaatcag
     2281 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt cccgtttttt
     2341 tctctaatac tcaggccaaa acgctgattt cacaacctct cctatacttt gtttcatttt
     2401 ccctatactt tgtttattta ttctcattat tgaaagcgct ataattacgt tgcgaatgaa
     2461 tcgaaagggg tttttagatg aaaaaacgaa aggggttcaa tcattttttt atgaatctat
     2521 ggcgttataa agcacttgtc ctgatggctg taccgggtat gctttggatg atttttttct
     2581 tttacatacc tgtaatggcg aatgtcgtag cattcaaaaa ttttcatatt tcagcagatg
     2641 ggttcttggc tagcttgagg gaaagtcctt gggtgggttg ggaaaatttc aaatttttat
     2701 tttcttcgga tcaagcattt ctcatcacta aaaatacaat tttatataat gtagcattta
     2761 tccttttgaa tctgctgatc tcagtcgtat ttgcaatcat tatgagcgag ttgagaaata
     2821 aacgactcgt taaagtttat caaacgatgt ccttactacc ttattttcta tcttgggtca
     2881 ttatcagtta ctttgtttac gcttttttaa gtcctgacaa aggaatattc aatcagtgga
     2941 tcactggaca tggaggagaa gctgtcaatt ggtataatga accgaaatat tggccgttta
     3001 tcttggtgtt cattggtaca tggaaaggga ttggttataa cagcattatt tactttgcat
     3061 ctgttatggg aatcgatccg acttattatg aagctgctat ggttgatgga gctagcaaat
     3121 ggcagcagat caaacatgtg acgataccac aacttattcc actaatgacg attttaacga
     3181 ttttagccgt aggaaacatt ttccgagctg attttggcct gttttataac attcctagga
     3241 attccggtgc attatatgat gtgactcaag ttttggatac gtatatctac aatggcctga
     3301 catccacagg agattttggt atgacggctg ctgcaggact ttaccaatca gttgtcggct
     3361 ttgtcttgtt gatgatcaca aatacgatcg cccgacgttt tgatagcgat tctgcattat
     3421 tctaaggagg aaaaaaatga aaaaaaagaa agtggagaga gtccaagtcc gctctttcaa
     3481 taagacggct aattttttct ctaatataat gattgcactc tttgctattt cctgtatttt
     3541 gccatttata tttgttatcg ccatctcttt taccgatgag tccatattgt cgactcacgg
     3601 gtacagtttt tggccagaaa agttcagtat atttggttat acgtacttgt tcgaccagat
     3661 gcaggataaa attttccaag cgcttttcgt cactgtactt gttactgttt taggtacact
     3721 gatcaatagc acggctactt ctttatatgc ttatgcgatt tcacggtcaa actttccatt
     3781 ccgtcgtttt tttaccgttt tttgtttgat tacgatgttg ttctcaccag ggatggtggc
     3841 caattatctt gttatgacaa atcttcttca gttaaaagat acgatttggg cattgatctt
     3901 gccaatggct gtcagtccct tcaatattat tgtgatgcgc acattcttta aacgttcggt
     3961 ctctgattcg attattgaat cggcacggat cgatggagct agcgaactgc gaatatttgt
     4021 tcagatcgtt ttaccgttag ctgttccagg tattgcgaca atcagtttat ttgccgcatt
     4081 gggttactgg aatgactggt tcaatgcttt actatacatc caagatgata agctagttcc
     4141 actacagtat cttttgatga aaatccaaag taatattcaa tatctgacac aaaattcagg
     4201 agcaggaagc caattgacag gcggattagc ctcagttcca ggagaatctg ccagaatggc
     4261 gatcgtagtc atctcgacgt tgcctattgc gatcagctat ccatttttcc agaagcattt
     4321 tgtcaaaggt ctaacaatcg gcggagtaaa agaataaaga aacaaaaata taaatcataa
     4381 aattaggagg aagaaaaatg aagttatgga agaaactagc ctttacaggt gtttcagcgt
     4441 tgttgttggg aacattagca gcatgcggag gttctggttc taaagaccag gcagaagcta
     4501 gcaattcgga cactttgcag atgtatcaaa tcggagataa accagataat ttcgatcaat
     4561 tgatggaagt agcaaacaaa cgaatcgaaa aagagatcgg tgtaaaagtc aatatcaatt
     4621 acattggctg gggcgattac gaaaagaaaa tgaacgtcat catttcttcc ggggaaaatt
     4681 atgatatcgc ctttgcgaac aactatgttt ccaatgccca aaaaggagcc tttgctgatt
     4741 tgactgaatt agcgcctaag tacgctgaaa aagcttataa tgatttagat gaagcatata
     4801 ttaaaggaaa tctggtcaat ggaaaattat atgctttccc agtcaatgga aatgtctttg
     4861 ctcaacaagt acttactttt aacaaagctt tactagataa atacgatctt tcaattgatg
     4921 gaatccaatc ttacgctgat gcagaaaaag tcttgaagga attccatgaa aaagaaccaa
     4981 acacggcagc ttttgccatt ggacaaggct tcaaagtcca agggaacttt gattatcctt
     5041 tagggaatac gttaccattt gctattgatc tggatggaga tgattcgaaa atcatcaacc
     5101 aatacgataa tgaaacgtat atcggactgt tacgtacaat gcatagctgg tacaaacaag
     5161 gactcattcc aagtgacgct gcaacgagca ataaggatta tccactagaa ggaaatactt
     5221 ggctaatgcg tggagaaaca caaggaccgt atgattatgg cgatacaatt ttgacaaatg
     5281 cagcaaaaca agaacttgtc tcaaaagcaa ttactgttcc actaaaatct acagctcaag
     5341 cacaaatggc aaattttgtc gtatcaaata cttcaaaaaa taaagaaaaa tcagtagaac
     5401 ttctagggct gcttaacagt gatccagaat tattgaatgg gcttgtttgg gggatcgagg
     5461 gagaagcatg ggaaaaagtc gatggttcag atgataaaat caaattgcta gatggttatc
     5521 agccaaatac acatatgtct gcttggaata caggaaacaa caagattcta tatacacaag
     5581 agtctattac ggatgacatg attgccaaac gtgatcaatc gattgctgat gcagaaactt
     5641 caccgatttt aggattcagc ttcaacactg acagtgtcaa aacagaatta agtaatattt
     5701 ctaatgtcat gaatcaatat ttagatggat tgaatacagg gactgttgat ccagatgaaa
     5761 cattaccgaa attgaaagac gctttggaca aggctggcta tgataaggta ttgaaagaaa
     5821 tgcaaaaaca atatgatgag ttccgacaag aataataatg aataaagaca aaggggttgt
     5881 gacaaaagac gtgtcacaac ctcttgttcc gaataaacgg tgttcaaaag ccagtttctc
     5941 ggtattaagc taaatatatg taaaaatgtg gaaagccata ttcacaaaat ttttcttact
     6001 actcgaaact aagcggctat ttcacaaact cttggtaaac ggtgttcaat cagctaaccc
     6061 tcggtattcg ctcacaaaac gacaaaatat gaggaactat ttttccgttt gtttctctaa
     6121 taatcaggtc aaggtgctga ttgcccaacc tttttttgta taatgagaaa gtataaactt
     6181 aatgtttgga ggagaagata tgttagggaa ggttttggaa gagttattta tccggatctg
     6241 ggtcattata aaactaacct tgtatttctg gatatataca tttgccggcg ggatcatctt
     6301 tggattaggt gcggcatgga aaacagtcaa tgaactattt tatttgtacg gattcgagta
     6361 taaagaaatc acaataaaaa gaggctggaa tatatataaa agaaattttt taagaggcaa
     6421 tctgctattt agtttatttt tatctggtac tgctttattg agttacaatt tatatttatc
     6481 tgttcaaatc aaagggctca tttttttagc tattgatttt attttacttt ttgccttgct
     6541 ctgcctattt gctacttatc aatacagtat gattttagat agtgaatata cgatctctgt
     6601 tcctaatctg ctaaagctaa gttttatttc ggttttttcc agcttttcgt cttttctaaa
     6661 aataattatt ggtagcggta taattttagg cgtgacttgg caattcaaag gattgatcct
     6721 gtttggtgtc attggtcttt tgacggtttg gaatggtacg atgacaacac attggcgtga
     6781 agaattagat aaacagttgg aaagttatga ataagtttcg gattctattt tcaaaaggaa
     6841 ccttactaaa tcgtctgttg cggaaatacg cggttatcat gatcggtgta acgatgactg
     6901 ctaccgttat ttttagtatc catacttggg aacaaaatca aaaacaggca gaaaacatga
     6961 caagtgatgc cgttcaaagc acaagcagaa tgctgaatga taaaacaacc ttgagtagga
     7021 tcatcaaaaa ccagcttgta gggaattccg aaaagatcga aaatgtgacg acctacttaa
     7081 caaaacccat agatcagtat ctgatgtatg tatacgaaca gcaaaacagt accgatgaat
     7141 tagtttcttt cccaaatcaa atcaaagatc tctacgcaaa ttatgaagaa ctaagtgcga
     7201 tatatatcgt gttgaatcaa ctaccggagt attatgaatc ttctagagaa aacaaaggtg
     7261 gggaaataaa aaaggggaca ccaaaattac tagacgcctt ttatatcaag ttgcctatca
     7321 cacaaggagg agctgagtca ggaagtattt ttattggttt tcaaaagaaa gaactagata
     7381 tgattttaga aaatctaacg tcattcaatg gactctcttt atacatgatt tctggaacga
     7441 caaatcgact atacacattc catgataata aaatagctaa agaaacgttt gaaagacaag
     7501 aaatgatcat cacagagagt ctgaagaaaa attcacaatt gccgattgac gatctggaaa
     7561 aaaacaattt tgttcaacac caagaactgt ctgatgatta tcgtatatta gctgtactag
     7621 ataaaaaaag tgtaaaaatc gaaacgggaa aaaatcttgc cccgctgatc attggtatca
     7681 ttattttaga caatatactg cttatctttc tttataaaac attcaaacga tattcacagc
     7741 aagtatctat cgtcatgcag gccatggatc aaacagcagc agggaattta gagacgcgga
     7801 tcgacataac aaacacggaa tatgaactga aggaactttc aatcggtatc aacgaaatgt
     7861 tggatagcat caatcagttt gtagaagaca tctacaaatt agaaatcaaa cagcaagacg
     7921 cacatatgcg ggcgcttcaa gcacagatca atccacactt tttatataat acattagaat
     7981 atatccgaat gtatgcaatc agcgaaggaa gtgaagaatt agcagatgtt gtgtatgcgt
     8041 tttccgcttt gcttcgaaac aatacgaatc aagagaaaac gatcacttta aaagaagaac
     8101 tggacttttg cgaaaaatat gtctatttgt atcaaatgcg ttatcctaat cgagtagcct
     8161 atcattttat gatcgatcct gatttggaaa agatcgaagt acctaaattt gtgatccagc
     8221 cattagttga aaattatttt aaacacggta ttgattttac tcgattcgat aatgcgctaa
     8281 gtgtcaaagt cttgcaggaa ggcaaacggg taaggatcat catcaaagat aatggaaaag
     8341 ggatgactga aaaacggcta aaacagattg aagaaaaact gtctcatcct aaagtggaac
     8401 ttcacggttc tatcggtctg caaaacgtga atgaacgttt acgtgccagc ttcggcagca
     8461 gttattatat gtcgcttgag aataatgaga caggtggact gaccgtttct attacattca
     8521 aggagggatg agcgtgtatc aagtgttatt ggtagatgat gaatacatga ttttgaatgg
     8581 gttaaaaaag ataattgatt ggcaaagttt aggctttcaa atagtagcaa cagcagagaa
     8641 tgccaaagaa ggactagctg tacttgaaca gcgacagatt gatttagtag taacggatgt
     8701 gacaatgcca gaaataaatg gactggaatt tattgaagcg gctcaaaaag aacgtcataa
     8761 ttttgaattt atgattttat caggatacca agaattcgat tatttgaaag gaggtatgca
     8821 attaggcgcc gtgaattatt tgatgaaacc cgtgaataag ttcgaattgg ttgaaagcct
     8881 caagaaaatc aaaacacggt tagatcagca aaatgaacaa aaaaatcaac aagaaatcta
     8941 tcaagaactg ttattcagtc aatggttgaa tgaagaattg gatgaaataa gtgaagaaga
     9001 aataatagga caaataagtg agaagcagca taggatagta ttgattcaac tggcaagaac
     9061 tcatgatact cttatcaata gatggttgca agaacggcaa caaatctttt actatcaacg
     9121 aaattacgga gaccgtgtat tgatcacgtt gttgttggag gcagatgaag ttgatggctt
     9181 ctgttgtttt gttcaagaaa actttcctga tcaggaatgg ctgatcagta tcggggaaga
     9241 gacagattct ttggacaaaa tccctgaaac attccaacaa gcgaaagaca atctacagtt
     9301 gcatcaattt tatggagata aagacgagca tgttttttac gcagtacaag cagaaacaaa
     9361 agatcaaacc atcgattttt caagtttcag acgcgtgctg caaaataaac aattggaagt
     9421 cgctcaaaaa atgatcactg atttttttga acagtttcag ttagcagcca tgatgccgga
     9481 agatatacga tattccagtt ttttactgtt tatggagatt caacgggaat tgattgatct
     9541 ggaagatgag gaatatttac aaggaatcga aaaaatccat caggcaaaaa cggtgcaaga
     9601 acttcagcag ctacttctgt cattcatcaa aaagcatcag agacagaaaa agtacagtga
     9661 caatgtggaa aaagtaatcg taatcctgca ccagcattat caagaaccac ttacactcaa
     9721 agaagtgtca gaaaacctgc atttaaatgt catgtatcta ggacagctgt tcaaaaaaga
     9781 aacgaaaaaa agtttttcag cttatttgaa ccatttacgt atggaaaaag cgaaacaact
     9841 gcttttgcac agtaatcaaa acatcaatga gatagcaagt gagattggct acaacaacac
     9901 cacgtatttc tctaaattat tcaagaaaat cgtcggcaga tcccctaaag agtaccgaga
     9961 aaacagtgga gaatcataaa agaaaaagcc aatgtaatga aataacttat aaaaaattgt
    10021 ctagaaatct aattttgggc tgttcttatt acttgaggca aggcagcttt ttcatgacct
    10081 ctcgataaac ggagttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg
    10141 tggaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct ttttcacgac
    10201 ctctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaaa aatggcaaaa
    10261 tatgaggaac tattttgcca tttgtctctc taatactcag gtcagaacgc tgatttcaca
    10321 acctctcaat aaacggtgtt caaaagcttg catctgcggt aataagacca accaaacgaa
    10381 aaatatgagg atctattttt gcttggctgt tcttattact tgatgcagga cagctttttc
    10441 acaacctctt gttaattata gtttgataag gaaaaaggca aatcttttgc ttgatatttc
    10501 ttagggtgag ggatgatccc taattggtaa gagatcgtgc cgcctttcag caaatcttga
    10561 tgggtaaaat ataatttcgt atattctttg tccatccgtt tgattttatg gataaattgt
    10621 tgctgcggcc gattaggcgt tgcttcaatc gtcaattcct ttccatttga caaaagcaaa
    10681 acagccttgt caattaatgg cataccgata acatattcac cggttccagg cgtgacagga
    10741 taaaatccca gactattgaa gatataccaa gcggccatac ttccattgtc ctcatctcca
    10801 gggtaaccca ttggagaatc gtcgaaggtc tgagtcatca actgtttaag cagtggctgt
    10861 gccatttcag gttgtcctat ataactgaac aagtaaggat agtgaaaact tggctggttt
    10921 gaaatagcta attgtccaaa ttctatcgcg gccatctcac tcatttcgtg gatctcaaaa
    10981 ccatatcctc ctgggtcaaa cgttgggggc tgattgcaca gctcaatgag tttttcttta
    11041 tatctctcac tgccaccata ttttccgatc agtcctgaaa aatcgtgata aacagcaaag
    11101 ctgctttgcc aagcacttcc ttctgcataa tcttgtcccc aacgattagc tgaaaatggt
    11161 tctctgaact tgccatcttt atcttttgaa cgcataaatc ctgactcata gtcaaagata
    11221 ttttgatagt tcaatgattg ctgctgatat ttcttcgtca tttcctcttc ttctagtacc
    11281 ttggcaactt ggctaataca aaaatcgcta taagcatagt ctaatgtgtg attgactgat
    11341 tcgtggtagt gattaggaac atagccatat gtcaagtaat cttctgttcc ttggcgtcca
    11401 taatttggat gtttgctttg aaccgtagca cctttttcca ttgccgttag aaattcaggc
    11461 atcaagtctc gtcggatatc ttttacagca gcatctgcaa tgaccgcatc gatcaatgtc
    11521 ccaggcataa gtccacgttc atcaggagac agccattttg gcaaatagcc actttctcta
    11581 tagctgttca aaaaaccttc cagcatttct tcgtattttt ctacagcaat caaagaataa
    11641 agaggataaa ccgttttata cgtatcccaa aacccattat ttgtatagag aacaccaggt
    11701 tttatagatc gactagtcgt atcatagtgg attttttgcc cactaggatc aatttcataa
    11761 aatgtttgtg gaaataaaaa cacccgatat aaattatgat aaaacgtccg caattgttgt
    11821 tgatcatggt gtgtgagctg tatacgatca aaataggact gccattgttc ttgactgtga
    11881 gcaaggtaag tctctttgct ttggttttgt tctagttcca ggttataagc tgcttgttcc
    11941 gagctgataa aagatgtccc aaatcggata gtttgttttt ttgtctgttc aaaatacaac
    12001 gggatcgagt cgctattttt gttggaaaca gctaactcat caattgttaa aggatattca
    12061 aatctcaata taaaataaaa agaaaaatca ggatcttctg aaccagcaaa atgtgtgatt
    12121 tttccttcca ctgtgtaagg atctataaca aagagctgat ggcgtcccgg aaaggataaa
    12181 agtaaaccgc tgtcattttt gctgtaatca atagataaga ttccgccata catgctaggt
    12241 atcaatgtag aacagatgcc ataacgtaat tgacagatcg acagatgagt cgggcaaaat
    12301 atactttctt ctgggcggta agaactttga gcatggaaaa gggtgttctc ctgtagtttt
    12361 ccattgatcg gcgtaaacag catatgactg aaatcaccca tccaaggact tggttgatgg
    12421 gtaagacggt agccttgaaa aacgcggtca tcaggatgaa accaccagct gccgttttga
    12481 tccgtagttt gcggtgcaaa ataattcatc ccaaaaggaa cgccagtata aggctggcag
    12541 ttcccattag agaaatttgg ctgattcgct gttgcatgtc tagtatctat ttgaccgata
    12601 ttcattttat ttctcctttg ttgattgtct tttgatgaat gaaactggaa atttactatc
    12661 ggatggtcga tcttttgtct ctaaccaagt gaccaaagca tctcccgcaa gcttacctaa
    12721 ctgttcaaaa tcttgtgcga tggttgtcag tggcgggtca acaagagctg ctgcttggat
    12781 atcatcaaat ccgataattg aaaaatcttc agggatatga taaccatgct gtctcaagag
    12841 attcatggtc tggatagcga caacatcgtt ttcacaaaca aaagcagtta cttcatactt
    12901 agtgatataa gaaagcaatt catctaatgt cgcgcttttg tcatccagtg aagtgtggaa
    12961 agtcaaacca gctttgtcca atgcttcaag gtaaccaagg taacgctgtc gaacggattg
    13021 tggatgagtc tgcgtaccaa acaaataagc gatttttgaa tgtccctgtt cgatcaaata
    13081 agaggcagca agcgcaccgc cttggaaatt atcggaaaga acggtaggaa aaggtaactc
    13141 atagattttt ttatctaata tgacgacagg gaattctttc aatgaaagct caaaaagtaa
    13201 gtcaagatgt tgttctgtat caagagcata ataaatcaat ccgtcaaatt cattgattag
    13261 ctcttttgct gttttatttt gtagaaagtc aagcgtcgtc atcatgacat caatctgttt
    13321 atcttgcatc acagggttca atcccttgct aaaatcacca acagacaaat cagcgacata
    13381 tggaagaaga aacagaatcc gtcgtgtttt tgtaggagaa gcaagaatct gttccggaga
    13441 agcggattta acgaaactgc ctttcccgcg aacgcgataa ataagtccta attgctctaa
    13501 ttcagtcaat gcacgtttgg aagtgattct gcttacttta taggtacgtg aaagttcttt
    13561 ttcagttggt agttggctgt ctacaggtaa ttcaccagtt aaaatagccg tttgcaaatc
    13621 attaaatatt gtctggtaca atggttgatt catataaaca cctcttaatg atatatcata
    13681 ttcaaataat accataatgg atgaaaaagt aaataaaaag atattttttg aaagcgtaga
    13741 cataattggc tgtatgaagt ataatcttta aagatatatc aaatagaaag aaggttttat
    13801 tagtggctta taaagatata ccaaaatcgg tgcagaaatt tatggaagat atcacacaaa
    13861 aatgtgggga tatccatgcg gactgggcaa agaattttac agcagcattt gccaatactc
    13921 tgttgacaac agtcaaaaag caaaatgacg gaaccacctt tttattgacg ggagacattc
    13981 cagcgatgtg gttaagagac tctactgcac aagttcgtcc ttatttagtg attgcaaaag
    14041 aagatgagga attagctgac atgatcagcg ggttagtcaa aaagcaattt ttttatatca
    14101 atatcgatcc atatgcgaat gccttcaacg aagaagctaa cggtgctggc caccaaacag
    14161 atcatacaaa aatgaacgat tggatctggg aaagaaaata tgaaattgat tctttatgct
    14221 acccaataca gttggcctat cttctttaca aaaatacagg aaaaactgat caattcaatc
    14281 aatcttttat tgaaggtatc aaaaaaatct tggacgtgtt caaaacagag caagatcatg
    14341 aacactctcc ttatacattt gtacgagaca cgacaagaaa agaagatacg ctgatcaatg
    14401 acgggcgagg tgcaaaaaca gcttttacag gaatgacatg gtctggtttc cgtcctagcg
    14461 atgatacttg ccgttatgga tatctggtac catccaatat gtttgcggtg gtcgtgttag
    14521 gttatgtgga agaaattttt caaacgatac tcgatgataa agaaattgta cagcaaacac
    14581 agaaattgaa gaaagagatc caagagggga ttcagaatca cggacgaacg gttaatcaaa
    14641 aagggcaaac gatttatgca tatgaagtag atggaatggg aaacgccaca gtcatggatg
    14701 acagtaatgt gcccaatctg atcgccgcac cttacttggg atattgttct gaacaagagg
    14761 aagattatct gcaaacaaga agaacattgt tgagtaaaga aaatccatat ttctatgaag
    14821 gaaagtacgc aaaaggaatt ggatcctccc atacgccaga aaaatacgtt tggccgattg
    14881 cattagctat ggaaggaatg acaacgtcag acaaatctga aaaagagcgg atattgaatt
    14941 tacttgtagc aactgacgca ggaacacacc ttatgcacga aggattcgat gtgaacaatc
    15001 ctgaaaatta tacgagagaa tggttttctt gggcaaatat gatgttttgc gaattagtca
    15061 tggattactt tgatattaga gtagaatagt aggaggtatc gtaaatgaaa aagaaagtat
    15121 atatcatttc tcatagccac tgggatcgtg aatggtacat ggcttacgaa cagcatcaca
    15181 tgcgactgat tgaattgata gacgatctac tagagctatt tgaagttgat ccgtccttca
    15241 acagttttca tttggatggg caaacgatta ttttagatga ctatcttcaa gtaaggccag
    15301 aaaaaagaca ggcaatccaa caagcaatca atgaaggaaa attgagaatt ggaccatttt
    15361 atatcttgca agatgatttt cttatcagct cggaatcgaa cgtgagaaat atgctgattg
    15421 gcatggaaga aagccgtaaa tggggaaccc ctgtcatgtt aggctatttt cccgatacat
    15481 ttggaaatat gggacagacg cctcaattaa tgaaacaagc tggtatttca gcagcagcgt
    15541 ttggccgtgg tgtaaaacca attggctttg ataatcaagt tttggaagca gaaaactatt
    15601 cttcccaata ctcagagatg tggtggaaag gacctgatca aacagcaatc tttggtctgc
    15661 tatttgccaa ttggtatagc aacgggaatg agattccagt agaaaaagaa gcagctcttg
    15721 ccttttggaa gcaaaaatta gcggatgcag aacaatatgc ctctacgaac cacttattga
    15781 tgatgaatgg tgttgatcat caaccggtac aaaaagatat atcaaaagca atccatctcg
    15841 caaatgaact gtttccagat tacgagttta ttcattctaa ttttactgac tatctggaag
    15901 cagttcaaaa agatgtgcca gaagacttag gctcagtaga aggagaattg acaagtcaag
    15961 aaacggatgg ctggtatact ttagccaata cagcatctgc tcgggtttac ttaaaacaat
    16021 ggaataccaa ggtccagcga cagctagaaa atattacaga accacttgcg acgatggctt
    16081 atgaagttag tggaaactat ccccatgatc aattagatta tgcttggaag acattaatgc
    16141 aaaatcatcc tcacgacagt atttgcggtt gttcagtcga ttctgtccat cgggaaatga
    16201 tacctcgttt tgaaaaagca gatgaagttg gaaagtatct tgcacaagat tccttagaac
    16261 agctgaccgc agcaatcgat acgaccggat tcccaaaaga cagctttcca tttgtcattg
    16321 taaatactgc aggaatggat aaaactggtg aagcagaaat aaccatcgaa ttagaaagaa
    16381 aaagattcgc tgaaggaata cctgaacaac tatatcaaga gttagaaaat ttgcctaaac
    16441 gtaagtatca tgtagaaaca aaaagtgggg ccactatacc agctatttta tctgaagaaa
    16501 ctgtccaatt tggttatgat ttgccgaaag accgtttccg tgtgccatat atggcaagaa
    16561 tgatcaaagt gactttacca ttagaaaaca tgccagcatt ttcttgggaa acattcgcgc
    16621 ttgtagaagg ggaagcgaaa gcagaagaaa aggaaacaat gattcatcag agtggacgaa
    16681 tcatcgaaaa cggccctcta catttgacga ttgaaaaaaa tggtaccatt acaatggaag
    16741 atcgcaaaaa caaacgaaag ttgaataatc tgcatatttt tgaagatatc ggcgatatag
    16801 gaaatgagta tatctttaaa caacctttct gtgatcagcc aatcctatcc agtaacaaag
    16861 aaaactctga agtaaaagta ttagtagata caccggaaat agcaaaaata agtattctac
    16921 aggaaatgga aatccctgtt tctgcagacg agcgtttaga aaaagaacag cagatggttg
    16981 tggaatttcg ttatcgaaaa gcagaacgat ccaaagaaaa acgaatcctt caaataaaaa
    17041 caatcatgac aatcagaaaa gatagtaaaa aaatcgactt tgaaacgact ctcgacaatc
    17101 agatgaaaga tcatcgctta cgtgtcttgt ttcctacaaa gttacatgtg gaacatcacg
    17161 aagcagatag tatttttgaa gtggtgaagc gcccaaacca tgtgtctaaa agttgggaaa
    17221 accccacgaa tccgcaacat cagcaagcat ttgtcaatat tcatgatgaa gagtacggtg
    17281 taacagtcgg aaactttggt ttgaatgaat atgaagtaac agaagacgga caaatcgcag
    17341 tgacgctact tcgcagtgtg ggagaactag gcgattgggg atattttcca acaccagaag
    17401 cacagtgtct aggtgagcat cggttcaatt acagcattga actacatgga ccagaagaaa
    17461 aattctctac ttatttgcat gcatacgcag cacagattcc tttttccact caacaaatca
    17521 aacatcatga aggaacattg atttcaaaac aacaatattt aacgataaaa agtgaaacgt
    17581 ttgcaattac agctttgaaa cgcagcaagt ttagtgataa agtagtagta agaggtttca
    17641 atatgtctag tcatttagaa aaattggaaa taactaaaga caatggaaaa actgtcatat
    17701 tgaatctgct tgaagaacca acgaagcaag cggttgtccc aataatccaa ccttatgaga
    17761 ttcggacaat cggattcgag gaggaaaacg aatgtatggc ggaatagaag caggcggaac
    17821 aaaatttgtg tgtgcggttt cagatggtgg agaaatttta gaaaaattaa gtatccctac
    17881 cacaacacca gaagaaacat tggatcaggt atttgaattt tttgatcgtt atgaattgaa
    17941 gggaatcggg attggctcat tcggcccgat tggtatcgac aaaacaaaag ataattacgg
    18001 atacgttttg gcaactccca aaaaagggtg ggcacaattc gattttcttg gcacaatcaa
    18061 ggaacgctac aatattcctg ctgcttggac aacggatgtc aatgctgctg catatgggga
    18121 attgaagcaa ggagcagcac aaggaaaaaa tagttgtatt taccttaccg tgggaacagg
    18181 aatcggtgca ggtgtcgtga tcaatgaaga aattttttca gggatcgcac atcctgaaat
    18241 gggacatatt tgggtgaaac gccatccaga tgaccattat gaaggtactt gtccttatca
    18301 taaagattgt ttagaaggat tagcagcagg accgtctatt gaggcacgca cagggatcaa
    18361 agggcaagat ctgccgcaag atcatcctgt ttgggctatt caggcttatt atattgctca
    18421 agcactgatc aactatacgt tgacgttagc acctgaaaag attattttag gcggtggggt
    18481 aatgaatcag gatcatctcc ttcaaaaaat ccgtcagcaa tttgttgaat tgatgggtgg
    18541 ttatatggaa acacctcaag taagtgaata tatcgttcgt tggggactgc cgaatgaaag
    18601 tgggattatc ggtagtttgc tgttagcaga acacgaacat caaaagtaag cagacaaatt
    18661 acacaaaaag aacagagtct tatcattatc tgctgataag actctgttct tttttatttc
    18721 tattcccata atccaggtaa agctttaccg actatttttt cttgagtaaa tggatggatc
    18781 atgtgcagtt ctaatgcatg gagcatcagt cgttcagctg ttctattttg gtagagaggg
    18841 tctccaacga tcggatgtcc cttatgagct aaatgaactc tgatctggtg tgtacgtccg
    18901 gtttccaaaa cacaatagac ttgtgtttgt tcagaaagta ctttgttgac agtgacatgt
    18961 gtcaaggcgc tctgaccatt tttatcatct acgacccttt ttctccgatc atggcgatct
    19021 cgtccaattt tattggtgat ggtcatatct ttggcaattt ctccccatac gatggcttga
    19081 tatctccgat agatcatttt ttgttccaac attcttccaa gtatcggcaa gacaaatgga
    19141 ttttttgcaa ataaaacggc accgcttgtt tctttgtcca agcggtggac aacgtaaggt
    19201 cgttgctgtt tagtgtcgag ataagcagct aaatgattga gtaaggtact gttttctgtc
    19261 ggttcatttg gatgagtctt cattcctgca ggcttatcca ctataatcaa atgctcatct
    19321 tcatataaaa cacggatgtg cttcgcatct cctaattgaa cttcttgata attataatct
    19381 gtttcttcga ataacaatgt gatctggtca ttgcccttta cttcatgatg gaacaaagca
    19441 ggttgttgat tgatccaaac gtttttacgt atacgcaaaa agtgtcgaac ctttctagga
    19501 actaaccatt ccttttcaag caagtcgcga attgttgttt caggatggtt cataggtaaa
    19561 gtgatcgtga ttttcacaag tgtactcctt tcagtgcaat cgcagactaa ttgtaccatt
    19621 tttaaaacat atttaagaaa aaacttacat ttttctgttc attcttatgt tactctactt
    19681 gcactaacgt aaaaaaacat attgaactac aacttaagca ggaagaaaac actctttcac
    19741 tatggtaaaa taagacgatt gaatcaaata gacaggagaa gaagcaaatg gattttcaaa
    19801 gcattttaga aaagataaaa gctgctttga tcagtttttg gcgttggatc agaccatatc
    19861 tcggacaatt ccatcgatgg cgaaagcgca tctggaaaaa atatcatgtg aacaaattga
    19921 ttttgctgat tggtttgatc tgtgtcttag tgacaagtat ctacctcttt attttagcaa
    19981 aacaagcaaa tgtcgaaacg ttaaaatcag gattaagcca atcgacagtc atttatgata
    20041 agaaaaacga agaagctggc acactgtacg cccaaaaagg aacatatgta gaattaaacg
    20101 cgatttctcc ctatatccaa gatgcagtaa tctcgacaga agataggaat ttttacgagc
    20161 atcacgggtt tgatatcaaa gggattgccc gtgcagcagt ccgaatggtc ttgtctggcg
    20221 gaacatcagg gggcggagga agtacgatca ctcagcagct tgccaaaaat gcgtatctga
    20281 cattagacca aacgtttgat cgaaaagcga aggaattatt tttggcaatc gagatcgaaa
    20341 agaagtacag caaagaagaa atcttgacca tgtatcttaa caatgcctat tttggcaacg
    20401 gggtatgggg tgtccaagac gcttccagaa agtattttgg agtagatgca acgaatgtta
    20461 cgctgtctga ggctgctact ttggcaggaa tgctgaaagg tcctggtatt tataatccga
    20521 tcgatcaccc aaaaaatgcg aatgatcgga aaaacacagt actaagtgta atggaagaaa
    20581 acggaaaaat cacaaaagaa caagaacaag cagaaagcca gacagatatc agcgcgtatc
    20641 ttaatgacac atacgataat tcagattctg gttatcgtta tccttattat tttgatgcag
    20701 tgatcgatga agcagttaac gagtatggtc ttgatgaaga agatgtcatg aataaaggct
    20761 ataaaatcta tacagcttta gatcaggatt atcaaaagca attagaagct acttatcaaa
    20821 acagtgcatt attcccagca aatgcttcag atggtgccat ggtccaatct gcttcagtag
    20881 ctttagatcc gaatacaggt ggtgtacaag cactagtagg acgaagagga gatcatgtct
    20941 ttagaggatt cagctttgct acacagatgc gtcgttcacc aggatcaacg atcaaaccat
    21001 taagcgtata tacaccagca ctggaagctg gatacaagcc ttccagtatc ttgaaagacg
    21061 aaccgcaaag ttattacgat gcgcataatt ttgacgggac ataccaagga gaagtaccga
    21121 tgtatcaagc tgttgcacaa agtttgaatt tgcctactgt atggcttttg catgagatcg
    21181 gtttacaaaa aggatatgat aaagcagaag aattcggcct gcctttagca aaatctgata
    21241 agtattatgg cttagccctt ggtggactgg aaaaaggagt atctccattg atcatggcgg
    21301 gtgcttacag tgcatttgcc aatgatggtc agatgtatac gccgcatctt atcacgaaaa
    21361 tcgttgattc gactggagcg gtgatcgtcg ataacacaaa caaaaaacca aaacaggtca
    21421 tatcaaagga aacagccgat gaaatgacca gcatgcttct tggtacattt tcaaatggaa
    21481 cagcggctgc tgcgaatcct gcaggttata cgatagcagg gaaaacagga accacagaaa
    21541 ctaatttcga tgccacaaaa gcaaatgacc aatggatgat cggttacacg ccagacgtag
    21601 tgatttcgac atggatgggt tacgaagaat ccagcaagct gcactatttg gaagggacaa
    21661 gtggaaatgt tgtcgggaaa gtcttcaaat cagcggcaga aggcatcctt ccgtatactt
    21721 caaaaacagg atttagtgta gcagatgcct atgccacagg aggacaagtg gtagctgcag
    21781 atcaagttgg aaacacagga aattctaatg gagagtctgg aaattggcaa gataacttga
    21841 atcaatatgg tgaacaagca aaagaagggc tgaaaaactt cggcgatatg gtgaaagaag
    21901 gcgttcaggg aattggtgaa gcggctaaag acctctggcg gaaatatcaa ggagagtagc
    21961 ccctttgaaa acgaataacc ccatgttaca atagagagta actactaaaa gaaaagaggg
    22021 cataacatgt caaacatcta cgatagcgcg aatcaaatcg aacgtgagat ccgtgaatta
    22081 ccagaattca aagcgttgaa agaagcgtac gagaaagtac aagcaaacga agaagcacac
    22141 aaactgttca aagacttcca agcgatgcaa atcgaattac aagaaaaaca aatgagtgga
    22201 caagagttca gcgacgaaga tgcaatgaaa gcacaagaaa tggctgtgaa aatccaatca
    22261 gaagaagtaa tcaatgactt gatgcaaaaa gagcaaggct tcagtacaat catcaatgac
    22321 ttgaaccgta tcatcatgac accagttcgc gacctttaca gcgaataaaa ctattttatg
    22381 aaacaacaga gggtgtgaaa gagacgttcg gccttgagcg atgacaaaat tgcttcttat
    22441 tgccgatagg ctgtctcttg aacaccattt accaagaggt cgtgagccta ccgtttaact
    22501 ctgagttaca aggaacaatt tcaaaaaaca gcttctcatg tttgatgact attgtgactt
    22561 gtgaccgagg agttggcagg agaacaccgt ttattcagaa ataggagcta cgacaaaagt
    22621 cttgtcatag ctcct
//
LOCUS       DADXTH010000050        22524 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.050, whole genome shotgun sequence.
ACCESSION   DADXTH010000050 DADXTH010000000
VERSION     DADXTH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.050"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            54..572
                     /locus_tag="IWI97_001786"
     CDS             54..572
                     /locus_tag="IWI97_001786"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBC2463284.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            700..1383
                     /locus_tag="IWI97_001787"
     CDS             700..1383
                     /locus_tag="IWI97_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBC2463285.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1692..1765
                     /locus_tag="IWI97_001788"
     tRNA            1692..1765
                     /locus_tag="IWI97_001788"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1726..1728,aa:Arg,seq:tct)
     gene            1924..2283
                     /locus_tag="IWI97_001789"
     CDS             1924..2283
                     /locus_tag="IWI97_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBC2463286.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2406..4037
                     /locus_tag="IWI97_001790"
     CDS             2406..4037
                     /locus_tag="IWI97_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBC2463287.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGELVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4099..4740)
                     /locus_tag="IWI97_001791"
     CDS             complement(4099..4740)
                     /locus_tag="IWI97_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBC2463288.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4926..5002
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5022..5330
                     /gene="rplU"
                     /locus_tag="IWI97_001792"
     CDS             5022..5330
                     /gene="rplU"
                     /locus_tag="IWI97_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBC2463289.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5357..5701
                     /locus_tag="IWI97_001793"
     CDS             5357..5701
                     /locus_tag="IWI97_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBC2463290.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5714..6007
                     /gene="rpmA"
                     /locus_tag="IWI97_001794"
     CDS             5714..6007
                     /gene="rpmA"
                     /locus_tag="IWI97_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBC2463291.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6441..9248
                     /locus_tag="IWI97_001795"
     CDS             6441..9248
                     /locus_tag="IWI97_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2463292.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9439..9930
                     /locus_tag="IWI97_001796"
     CDS             9439..9930
                     /locus_tag="IWI97_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2463293.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10033..11022
                     /gene="manX"
                     /locus_tag="IWI97_001797"
     CDS             10033..11022
                     /gene="manX"
                     /locus_tag="IWI97_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBC2463294.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11055..11858
                     /locus_tag="IWI97_001798"
     CDS             11055..11858
                     /locus_tag="IWI97_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBC2463295.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11879..12793
                     /locus_tag="IWI97_001799"
     CDS             11879..12793
                     /locus_tag="IWI97_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBC2463296.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12876..13250
                     /locus_tag="IWI97_001800"
     CDS             12876..13250
                     /locus_tag="IWI97_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBC2463297.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13389..13634
                     /locus_tag="IWI97_001801"
     CDS             13389..13634
                     /locus_tag="IWI97_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBC2463298.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13806..14870
                     /locus_tag="IWI97_001802"
     CDS             13806..14870
                     /locus_tag="IWI97_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBC2463299.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15116..15541
                     /locus_tag="IWI97_001803"
     CDS             15116..15541
                     /locus_tag="IWI97_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBC2463300.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15534..15983
                     /gene="nusB"
                     /locus_tag="IWI97_001804"
     CDS             15534..15983
                     /gene="nusB"
                     /locus_tag="IWI97_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBC2463301.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16080..16934
                     /locus_tag="IWI97_001805"
     CDS             16080..16934
                     /locus_tag="IWI97_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBC2463302.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16935..18272
                     /locus_tag="IWI97_001806"
     CDS             16935..18272
                     /locus_tag="IWI97_001806"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBC2463303.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18277..18501
                     /locus_tag="IWI97_001807"
     CDS             18277..18501
                     /locus_tag="IWI97_001807"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBC2463304.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18503..19393
                     /locus_tag="IWI97_001808"
     CDS             18503..19393
                     /locus_tag="IWI97_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBC2463305.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19402..20220
                     /locus_tag="IWI97_001809"
     CDS             19402..20220
                     /locus_tag="IWI97_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBC2463306.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20327..20776
                     /locus_tag="IWI97_001810"
     CDS             20327..20776
                     /locus_tag="IWI97_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBC2463307.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20794..22476
                     /gene="recN"
                     /locus_tag="IWI97_001811"
     CDS             20794..22476
                     /gene="recN"
                     /locus_tag="IWI97_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBC2463308.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
BASE COUNT         7329 a         3746 c         4996 g         6453 t
ORIGIN      
        1 ttatcgttat tatatgatta agaaggattg aataaagaaa gaagttgaaa acgatacctg
       61 attacaaaaa aataactttt ataagttcta taacgttgat tatattttta attcttattc
      121 ttctcgtcgt cttggttccc cgaatttttg gtttgactcc ttatattatt gaggacaaag
      181 aaatgagtcc atattatgca caaggaaatc tgatatatgt acgaccgaaa aatacaaatg
      241 agattttagt cggagatatt attacatatt atgaaaattc aggaaaaaaa atctcgacgc
      301 gtcgcgtaat agccgttgaa aacaaccacg aagattttta tacaaaagct gatacaaaga
      361 cttatattga gcctggagta gtaaaaaaga gaaatattat cggtagtccc atctttcaga
      421 ttccatatat tggactagtc ctttcaaaaa cggcatttgc atggattcag gggttattct
      481 ttacgattgc tttttgccta acaggaatca cagcatggaa tacctttgta gagttgaaaa
      541 agaagcggag acgaacacag aaatttttgt aagaatgtta aaaacataaa tgtattccag
      601 agatccaacg actcttctga acatgaaaca cgagaaaaca ctcatgcagc ttgtagattt
      661 agtcagttgt tcgtataatg tgtgaagagg aggagtagta tgcgcaatga aatgatgcat
      721 cgtcctgtag tcaaagaaga actattggat tttatgcgta cccagcaaaa aaaattaccg
      781 ggtgagctag gcaaactgga agaagaagct catgtagcag aagtaccagt tattccccat
      841 gaaacagttg tatttttgaa atttttactt ggacagcttc agcctgaacg aatcttggag
      901 atcggagcag ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg agaaaacgct
      961 catgttacga caattgatcg tttcgatgtc atgatcgaga aagctaaaaa aaactatgaa
     1021 cgtctaggac tgacagataa agtgactttg cttgaaggtc aagcagcaga tatcttgccg
     1081 gagttgtctg gtccgtatga ctttattttt atggatagtg caaaatcaaa atatattgaa
     1141 tttttacctg aatgcttacg tttgttaaga aaaggtggcg ttttgatggt agatgacatc
     1201 ttccaaggag gaacgatctt gctcccagat gaagagattc cccgtggcaa acgagctatc
     1261 catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc atcccgattt gacatcgaca
     1321 atcttgccat taggtgatgg tgtgatctta atgacaaagg aatcagaaac aatcgagttg
     1381 tgatacatga ttatggcagc ctgtagataa tcgattgaat cgagggctca attcgaactt
     1441 gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata caggaaatct aaataaacag
     1501 gagtagcatg aagtcaatgt agctcctgtt ttcaattgat aaccttagaa cagctgaagg
     1561 agaatgtctc gttttaaaag attgatttag tagttgggcc aaaataattc attatttgct
     1621 aattaaataa ctatttcgta ttgacagaag caatttaatt atctataatt attgatgttc
     1681 cgctgattct tgtcatagta gctcagctgg atagagcatc cgccttctaa gcggacggtc
     1741 gggggttcga atccctcctg tgacgctaaa aaaccgttag aaactgaggt ttctaacggt
     1801 ttttctttgt tttttgtgcc aatattgttt gttttcaaag gcaaataaaa taaaaaaaca
     1861 tccttatcga gtacaattat aaggagcaaa aaaataaaac tcatcagaaa agaggaagaa
     1921 gatatgacga tactatctag aattttagtg actctagttg caattgaatt tttttatatc
     1981 atgtatatcg aaacggtgag aactgattct gacacgacaa gtagggtttt taaaatgagt
     2041 aaagaagagt taagcagaaa atctgttcaa acattgttta agaaccaagg agtgtacaat
     2101 ggactattag gagtgggact actttatgga gcttatcttt cttccgcatc taaagaaatc
     2161 acaagtatgc tattaatatc tatttttttt gttgcattat atggcagttt gactagcgat
     2221 cgttggattt tactcaaaca aggtgggcta gctctgttgg cattgctttc tattttctta
     2281 taaatataga aaaaataaga atttatctaa caggaaactt tgtgaaaaat attactttat
     2341 ctcatgaaat atgatattct aatgaatggt attttagaaa atttcatgaa aaaggagtta
     2401 gataggtgtt caaaaattat gtttcctcac taaaaaaaga attttcaggt tatgatcttt
     2461 caagatttca gaaagatctt ttggctggga tcacagtagc tgctgtagca ttgcctttag
     2521 ccttggcttt tggggtatca agcggagctg atgcggccgc aggtttgatt acggctatca
     2581 ttgccggtat cgttattggc ggtttatcag gcggttttta tcaaatatca ggtccgacag
     2641 gtgcgatggc agctatcttg atgtctattg cggcaacaaa gggaatgcaa ggtgtattgc
     2701 ttgctacttt tttagcgggt ctatttttac ttgcagcagg acttttccga ttaggaacac
     2761 tgacttcttt tatcccagca cccgtaatta cggggtttac ttcaggtatt gctatcatta
     2821 ttgcattagg tcagatcgat aatttatttg gtgttcactc ggaaggtaca aacgttattg
     2881 aaaaactaag tagttatcaa acgataggat ttccgataaa cacagcctct ttgatcatgg
     2941 gagagctagt cattttaggg atgtttatct atccaaaaaa atgggcgaaa cgaatgccta
     3001 gttcgctgct ggctatcatt ttggcaacag cagttatgct attgttccat ttaccagtag
     3061 caactgtggg taagattcca caaacattga tcagtagtaa tcgtttgaat atgggagatt
     3121 ttagcttttc tgccttgcaa caagtggcag ttccagctat tagcattgct ttattaggaa
     3181 tgattgaaag cttgctatgt ggtgcttcgg ctggccgaat ggcaaataag cccttagata
     3241 gcaatcaaga attaatggcg caaggaattg gaaatctgtt gcttcctttc tttggcggga
     3301 ttcctgctac tgcggccatc gcccgtacaa gcgtggccat caaatctggt gcacaaacta
     3361 gattagctgg tatgatacat gcagttgtct tactgctctc catgcttgtt tttgcgccag
     3421 tgatgtcaca gattccaatg ccagcacttg caggtgtttt gattgtgacg gcttggcgta
     3481 tgaatgaatg ggaaacgatc aaggagctat ttactaataa atattggtca gcagttctat
     3541 tgtttatcct tacgatgatt tgtacagtta tctttgattt aagtattgct atcgtgatcg
     3601 gggtgatcgg cggctgtgta ttttttgttg tcaaaagcgc tgctatcacg atttcattag
     3661 aaacgatcga ttggcaaaga atggggctgc cagaaacgga aaagctgaat aactggacag
     3721 tagtgtatat tagtggtcca ctgtttttta tgtcttcaga aaaactcaaa aataaattgg
     3781 aagaactaaa tcatcacgat ggtgtcattt tctccatgcg aggcgtacca agtattgatt
     3841 tgacggcacg tgcagttttt gatgaatttc aagaaaaagc agagcaaaga ggacaaacga
     3901 ttatctatac ttccttgcag ccagaagtag aaaaacaact ttctcacttg tgggaacata
     3961 cattcacgca tcaacatcaa acagttgctc atgcgttgac agctttacat gcgaaatata
     4021 ctgcaattag ccattaaaac aatgaaaaag aacagacaag actgtaaact gaagatttta
     4081 tggtcttgtc tgttcttttt aatattgctg taaaagtgaa gctagtactt gccagtcttg
     4141 atggataaca ggtacaaggg agcgattgta gaaaatagct gctggatgaa aaagtggaag
     4201 aattcgatag tttttttctg accattcgta ggcatccttt tgttctgtta attgtaagac
     4261 aggccctgta tagagttgac catgattttt ggtaaccgta tattctttgc caagtaaacg
     4321 ttgaagacca atgtttccaa gtgtcgcaat taattttggc tggaccttca tcagttcgta
     4381 atccaatatt ggtgcatgcg caaatacttc ggaacgagta ggtgtccgat ttggataaac
     4441 agtttctgac tgttgtgtcc gtttattgat ccgatgagtc acgcgatacg ggcggctcct
     4501 tacagcactg gtgatataca cctcttctcg tgttaagcca gttgaagaaa gtgcctgcat
     4561 caattctttt ccagcttgac cgctaaaagg aacatgcgta tcaatctctg ttttccctgg
     4621 ggcttctccc acgagcatca gtttaggatg aagaggtcct tgcccagcga taaatccttc
     4681 tacaggacgg ccgttcattc ttaaagaagc ttcttgtatc agttcttttg ggtattccat
     4741 ttatgctcct cctttttctt ttattataag aataaaagaa acacaatgcg actattttat
     4801 ttggtagaac tgtgaggata gaaaagcaga agtattgctt gaatttttgt tttacttttt
     4861 gattttaaat ttagttgact aacggttgct gttctggtaa gctagtactg ttgtaattgt
     4921 ggactccaca gctacaaccg ctcagaacaa gtaataagca cagcatatgc tgtcacttcg
     4981 gatggcgagt ctaagtatat cagaaggagg tgctttatag catgtacgca attattaaaa
     5041 ctggtggtaa acaagtaaaa gttgaagtgg gacaagcaat ctatgttgaa aaacttgacg
     5101 ttgaagcagg agaaaaagtt gtttttgacg aagttatctt agtaggcgga gaatctacaa
     5161 aagtaggcgc tccaactgtt gcaggtgcaa ctgttgaagg tactgttgaa aaacacggca
     5221 aacaaaagaa agtcgttact ttcaaataca aacctaaaaa acattcacac cgtaaacaag
     5281 gtcatcgtca accatataca aaagtggtta tcgacgcgat caacgcttaa tagcgtttga
     5341 gaaagaaggc ctatatatga tcaaaggaac atttaagcga aatgacgcag gtcagatcgt
     5401 ttcattcact ttgaccgggc atgctgatgc tggtccttat ggaagtgata tcgtttgtgc
     5461 gggtgtttct gcgttagcga tcagcactgt caacgggatt gcctcattag ccggttttga
     5521 gccgattgtt gaaatgaatg aagaagaagg cggttatctt tataccgaag tcacttcagg
     5581 aatgacacaa gaacaaaaca atattgctga gattctatta gaaaatcttt tattaggatt
     5641 acaatcgata gaagctgaaa actcagaata tatccaaatt aaaacaatta atgataaata
     5701 ggaggtgcag accatgttgt tatctatgaa tctacaatta tttgcccaca aaaaaggtgg
     5761 cggttctaca tctaacggcc gtgactcaga atcaaaacgt ctaggtgcta aacgtgctga
     5821 tggacaaaca gttacaggtg gatcaattct ttaccgtcaa cgcggtacaa aaatctatcc
     5881 aggtgtaaac gtgggtatcg gtggagacga cactttattt gcaaaagttg atggcgtagt
     5941 acgtttcgaa cgtaaaggcc gtaacaaaaa acaagtgtct gtttatccag tagctcaaga
     6001 agcttaattg aatcttatga ttaaagaggt tgtgacaaga cttttgtcac agcctttttt
     6061 gtttataaaa ataaaagttg tagtggaggc tacgaagttt actttgtctt tttacactta
     6121 atattttttt gccaatttgg aatcaggatg ttttgataac tatcgttttc aattgatgga
     6181 agctatattg tttgctatca gtgaaataat tatatggtaa acagtgttct ttttcttgtt
     6241 tccattataa aaatcgtatc gggatttttc gttcttttaa attgaacatt tctatctatc
     6301 cgcgtgttat caacaaaaca aaatcaggta aatagttgac gaaacagaaa taaatacata
     6361 gtattatgtt ttgaagccta agtacaatag tctatagaaa aggagcgata ccattggcaa
     6421 aacgcattga ccgaatttat atgtatgtca aagaaaaaac cgctcattta acaaaggcag
     6481 aatatgatca aggagtaaca acacaggaaa ttgctgaagt gttagggatt cagcgaacaa
     6541 atagcagtaa agatctgaat cagctagtgc gcgaaggaaa actgataaag acagacggac
     6601 gaccagtacg ttacatatat caaacactcg ttacgcacgt aaagccagtt acaaaacatg
     6661 tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa tacactccaa atagatacta
     6721 aggatatctt tgcaaaaatc attggagcta acggcagcat gaaaaattca gtggaacagg
     6781 caaaagctgc gattttgtat ccgccaagag gattaaattg tttgattaca ggacctaccg
     6841 gatcaggaaa gacttatttt gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa
     6901 tagatgagga aacggaactg attgttttca attgtgctga ttatgccaat aaccctgagc
     6961 ttttgatgag tcatttattt gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa
     7021 aaatggggat tatcgatcaa gcggatggcg gtatgctgtt tttggatgag attcaccggt
     7081 tgccgcctga aggtcaagaa atgatctttt actttatgga tcatggaact tacagtcgtc
     7141 ttggggaaac aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg accacagaag
     7201 acccaggttc ttctctatta gaaacttttg ttcggcgtat cccaatcaat atcaaacttc
     7261 cggcttttga aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc atgattgccc
     7321 acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga agatgtagca aaagcattga
     7381 tcggcagtgt gacctatgga aatataggac aattgaagtc aaatatccag ttagtatgtg
     7441 cacgaggatt tctaaatcat atgcaatcac ccgaaatctc gatcacaatc gatgatttga
     7501 cagaagggat tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca atggcagagc
     7561 tttctaaaat attggagcca aagataactg tagcaccaaa tgatacgatc atgaaaatcc
     7621 aatcggactc ctatgagtta ccttataatc tatatgatat catcggagat aaagcagctt
     7681 tactgaagtc ggatggtttg gatcaagaag caatcaatca tttcatttct acagatatca
     7741 acatccatct gaagtctttc tataaagacc atggtttttc ttttaatgca gacagcaaat
     7801 tagcagaatt tgtagatccg aaaatcatag aagtgaccaa tcagatttat gtaatggtca
     7861 aaagatcttt accatatgag tttcagcaaa actttattta tgctatgagc ttgcatatca
     7921 gttctttttt gaaaaaaatc aacttaggag aagaacggca tacgaatgat aacatccgtg
     7981 agatggcttt tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg
     8041 aaacgtattt tcaagtggag attccagaaa gtgaagacta ttacttgacc gttctactcg
     8101 tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca gctcacggaa
     8161 acagcacagc aagcagtatg gtccaagttg tccaacagtt gttagatgca gagaacgtac
     8221 gagcagtaga catgcctttg gatatggatc cgaaaacagc attggaccga attgaacgca
     8281 atgtacaaga agtagatgaa ggaagcgggg tcatactctt agtagatatg gggtcccttg
     8341 cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc agtacgtact gttgatatgg
     8401 taacaacttc tctagttttg gaaactgtac gaaaggtctc agtattaggg acagatctag
     8461 atatgttgca tgaatcattg aaaaattttc gaggttacgc agaaatatct tctgtagaat
     8521 ctgttcagga agcaaaacct attcataaga aaaaagctgt tttggctatc tgcgcatctg
     8581 gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag
     8641 aaacagaatt agaagttcta gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta
     8701 tccaaaaaga atatcagatc atagctacta ctgggattac tgatttgaat atccaagccc
     8761 catttattcc tttagaacgt tttattgatc aaaatatcga agtcattttg gatcagttgc
     8821 tgatggaatc agaattggag gaaactgaat tcatctccct agatgaagag tcagctaaaa
     8881 atacgtgtgt tgagtttatc tctgataact tcatctttat taatggtagt aagttaatcg
     8941 atccaatgtg gcaattttct acacaaatct ctcagacgac gggaatcggc gatgaagaat
     9001 atggattcaa aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt attattcgga
     9061 atgaaccatt gactgtggaa gaaaacgaat tgacaaatac aacgaatgat cctctttata
     9121 gccaattggc agcctcagtt gttctgttgg aagatcagat aaaagtaaaa gtgccgatag
     9181 aagaaatgta ttatctgcta cgtctagtcc ataatcagtt agataaaaaa gaatacactg
     9241 tgccatgaat acacaaaaac gatacacttt tatagtgtat cgtttttgtg tattggttaa
     9301 gaatccttta aattcaatta gtgtatcgta aaaaagttgg cacgcttttt gcttatagta
     9361 gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg ggtttctgtg aagtagtgag
     9421 gaagaaggag ttttgcagat ggatattcga ttggcacgta tagatgatcg tttaatccac
     9481 gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc
     9541 gatgtagtcg ctcaagataa attgagaaag tttctactaa aagaagctgc accgccgggg
     9601 atcacagcaa atgtaataac cgtgcagaaa atgattcaaa tctatcatag tggattatta
     9661 tcacgagcaa aagtgatgct tttattcacc aatccccaag atgttgaagc ggttgtccgc
     9721 tgcggtgtac agttgaagtc gttgaatatc ggcggtatga gttttactga aggcaaatca
     9781 atgattacca attttgtttc agtaaatcaa caagatattg attcctttcg ttacttgaac
     9841 agtcaagaag tggagttaga gattcgaaaa gtacctgctg acaggaaaat ccttttgatg
     9901 gaactactag cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa
     9961 ttctgttgta atggttagca attattaata tttttgaatt tgttacaaca aaagtaaata
    10021 ggaggtataa atatggtagg gattatccta gcaagtcatg gacaatttgc tgaaggaatc
    10081 ttacaatccg gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa
    10141 ccaagtgaag gtccagatga cttgagagcc aaattggaag aagcggttgc ttcttttgat
    10201 aatcaagacg aagtgttatt cttagttgat ttatggggcg gaacaccgtt caatcaatca
    10261 aacacattat tcgaagagca caaagataaa tgggcaatcg tcagcggttt gaatttacca
    10321 atgttgattg aagcctacgc atcacgcttt tcaatggaat ctgctcatga aattgcagca
    10381 cacatcattg aaacagcaaa agacggtgta aaagtaaaac cggaagaatt agaaccggca
    10441 gaagcaccga aagcagcagt tgaagacgca caacctaaag gagcacttcc agaaggaact
    10501 gtggtcggag acggaaaaat caaatatgta ttagcacgtg ttgactcacg tctgttacat
    10561 ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa accgtattat cgttgtttca
    10621 gatgcagtat caaaggatga tctgcgtaag agactaatcg aacaagctgc tcctccaggt
    10681 gtaaaagcaa atgttattcc tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt
    10741 ggaaatacca aagcattgct tttatttgaa aatcctgaag acgtattgac agctgttgaa
    10801 ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc
    10861 gttgtaagta aagtattgtc tatgggacaa gaagacgttg aagcgtttga gaagcttgaa
    10921 caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg attcacgcga taacatggat
    10981 gatattttga aaaaagcaaa agctgaatta gcaaaagcgt aataaataat attaaaattt
    11041 ataggaggct tatcatgtct attatttcaa taattttagt cgtattagtt gcctttctag
    11101 ctggtatgga aggtattctt gatgaatttc aatttcatca accactagtg gcatgtacat
    11161 tgatcggttt agttaccggt aacctcgaag ctggtatcgt acttggcggt acgcttcaga
    11221 tgatcgcttt aggttgggca aatatcggtg ccgctgtagc accagatgct gcattagcat
    11281 cagtagcatc agcaattatt ttagttttag gtggacaagg tgttaaaggt gttccttctg
    11341 cgatcgcgat tgccgttcct ttggctgttg cagggttgtt cttaacaatg atcgtacgta
    11401 caatcgctgt tccaatcgtt catttgatgg atgcggctgc tgaagaagga aacatccgta
    11461 aggttgaaat gtggcacatc attgctgtat gtttacaagg ggttcgtatc gcggtcccag
    11521 cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag
    11581 catggttaac agatggtatg gctatcggtg ggggaatggt cgtagctgta ggttacgctt
    11641 tggtaatcaa catgatggct acaaaagaag tatggccatt cttcgttatt ggtttcgttg
    11701 tagctgcaat ttctcaatta acattgattg ctctaggtgc tttaggtgtt gcattagctc
    11761 ttatttactt gaacctttct aaaatgggtg gttcatcaaa taatggaggc ggaagcaact
    11821 ctggtgaccc tttaggcgac attttgaatg actattaatc ttgaaggagg aaaataaaat
    11881 ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac
    11941 cttcattcaa ggttcatgga actacgaacg tatgcaaaac ggtggctggg cattttctat
    12001 gattccagca atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag cattaaaacg
    12061 tcacttggaa ttctttaata cacacccata cattgcttct ccaattttag gggttacatt
    12121 ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt
    12181 taaagtaggt atgatgggtc ctttagctgg tgttggtgac ccagtgttct ggtttacagt
    12241 acgtccaatg ctaggtgcac taggtgcttc attagctatg ggaggaaata tccttggacc
    12301 aatcatcttc ttcttaggtt ggaacattat tcgttgggca ttcatgtggt atacacaaga
    12361 atttggctac aaagctggtt ctaaaattac tgatgacctt tctggcggat tgttacaaga
    12421 cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta gctgcccttg tacaaagatg
    12481 ggtatcaatc aaattcttac cagttgtatc aactgtaaaa ttagataaag gtgcttacat
    12541 cgaatgggat aaattgcctt caggtggtga aggaatcaag agtgcatttg aacaagttaa
    12601 taatggctta gccctttctc ctgaaaaggt aacaacatta caagacaact tagaccaatt
    12661 gattcctggt ttagctgcat taggattaac attgttatgt atgtggttgc ttaagaaaaa
    12721 ggtttctcca atcgttatca tccttggttt attcgtagtc ggtgttgttg gacacgtcat
    12781 cggactttta taatccataa aaatatagct cgggcgttgt cccgagcttt ttttatatag
    12841 tatactgttc ataaacaaat atctggaggg agaaaatggt tcaatcaatc aatacaaaag
    12901 tagacttggt aattgatgct acttcgttta cgggtctgac agattacggg caaatcatga
    12961 taggtgataa tggttttgag ttttaccatg cgcgagatag acgcaaatat atccaaattc
    13021 catgggaaga agtggattat gtactagcat cagtcatgct aaagggaaaa tggattcctc
    13081 gatatgccat tcaaacgaaa aaaaatggta catacacatt ttcttccaaa gaacctaaaa
    13141 aagtgcttcg tgcgatcaga gaatatgtag atgaagatag aatggtacaa tcattgagtt
    13201 tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga
    13261 aaacttctaa gaggaggttt tctttatttt tttactttgc agaagaaaat atgaaatagc
    13321 aaaaccaatt atttgtcccc tttaaatccc atatgctaaa attgattagc aaagaaggag
    13381 gttatattat gagttggtta tggacattga ttgttggagc cgtgatcgga gcaattgcag
    13441 gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac gaatatcatt gctggactag
    13501 tcggttcaag tattggacaa gcgattttcg gcgaatgggg accaaaagct gcaggtatgg
    13561 cacttattcc atctatttta ggcgcagtga ttttggtagc agttgtctca tttttctttg
    13621 gaaaacgatc ataagttttt acaaaatagt aaactaaaag gagttgcagc gagactgtga
    13681 ctcctttttg gctttattta tttaaaataa aaagaagttt tccggaaatt tttatttaac
    13741 tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc gtgcaaagaa agagggagtt
    13801 tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat gcaagaagaa aatttggatt
    13861 catttttggt taccagtccg tacaatcttc gttacttaac gaactttaca ggtacgacag
    13921 gattagctgt tattactttg gaaaaagcat tttttattac tgattttcgt tataccgagc
    13981 aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa tgtcggacct atttttgatg
    14041 aggtagcaaa cttagtacgc aaagaagaac tgcactcatt agcttttgag gaaacaacag
    14101 tctcattttt agaatattcg gttttagaag aaatcatcga tgcggaatta gtacctgttt
    14161 cacagatgat cgaagaactc agagaagtga aagatgaaga agaaatcgct attatcgaaa
    14221 aagcatgtca tattgcggat atggcatatg atcacatcct gaaaatgatc caaccgggca
    14281 tgactgagat tgaagtcgca aatcaactag acttttatat gcgttctctt ggcgcttcag
    14341 gtgtttcttt tgatacgatc gttgctagcg gattgcgttc ggctatgccg catggtgtag
    14401 caagcaaaaa aatcatcgaa caaggtgact tgatcacatt ggactttggt tgctattacg
    14461 aagggtacgt ttccgatatg acacgtacat tcgctattgg tgatcctggg gaaaaattaa
    14521 aagaaattta tcaaatagtc ttagatgctc aattagcagt tattgatgct gcaaaaccag
    14581 gaatgactgg tgtccaatta gatgcagttg ctagagatta tatttcaaaa catggttacg
    14641 gggaagcatt tggacattct acaggacatg ggatcggttt agagatccac gaaggaccaa
    14701 acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa tgtgatcact gatgaaccag
    14761 ggatctactt accaggaatc ggtggagtac gtattgagga tgacttgttg atcacagaag
    14821 atggaaatcg tgtgttgact cgttcaccaa aagaattgat catcttatag tttcatcaat
    14881 agaagaagta agcgtaaaaa tcagtgttca cagactttgt tatagaaaat cactcgattt
    14941 aatgatacac tatagaagaa cttttaagct gtaagagata ataaagttgg gtgttttctc
    15001 caattcatag cagtttgcag ctgatcggcg gcagttttct taccacgtga tcactatgcc
    15061 gcatcataga gaattcggat gtatctcgtg cttaggcaaa gggaggaata aataaatggc
    15121 agatgaaaaa aatttgattt taaacgcaaa ccaagattta ggagagatcg tgatcgctcc
    15181 tgaagtcatc gaagtaatta tcgggattgc agcttctaaa gtagaaggcg tatacggaat
    15241 gcgcggaact tttgccagca atgtcactga attattagga cgtgcagctc acggaaaagg
    15301 tgtttactta gcaaacgagg aagatggctt gaaagtagat ctttactgtt atctagaata
    15361 tggtgtttca gttcctaaag tagcaatgga aatgcaggaa cgggtcagcc agcaagtcct
    15421 tttcatgacg gatatcgatt tatctgaagt caatatccat gtagtagctg ttgttcctga
    15481 aaaattagaa caaccaacaa tcgaagaact ttttgaagac gaagaggaag agaatgaata
    15541 aagaattatc aagacacgag attcgggaaa tggcattgca ggctttattt ccattagact
    15601 tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc aatcgaatta gaccaccgcg
    15661 acatgatcaa cgaagatgaa tcagagtttg ttcctgttta tcttgatacg ctagttggcg
    15721 gtgtctgtgc aaaaaaagat gaattggata aagtgattga aaaacattta aaaaacaatt
    15781 ggcacatcaa tcggatctca aaaatggatt tggtgatctt gcgtatcgcg atttttgaaa
    15841 tgacttatgt gacagatgta ccagcggctg ttgctttaaa tgaagcaatc gagttggcaa
    15901 aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg tgttttatcc aatgtattga
    15961 aagaattaga agctggggcg taagtcctag cttttttcta tgaatcatca taaacctgtt
    16021 ataaatcggg aaaattctat ggtagaatga aaacacaaaa aatatctggg agtggttaga
    16081 tggcagaatt gattaacgga aaagaattag ctgaaaaaat gcaggcagag attgctgaaa
    16141 aaatccaaaa attgaaagat aatggtatcc atcccggatt agtcgtttta ttggtagggg
    16201 aaaatccagc cagccaaatc tatgtaagaa ataaagaacg tgtagcaaaa gaaatcggaa
    16261 tccattcatt ggtagagcgt tatcctgaga cgatttctga agacgaacta cttgcagaaa
    16321 ttgaaaaata caatcaagat cctagttttc atggtatatt ggtccaatta ccattgccaa
    16381 agcatataga tgaagaaaag atcctgttag ctatccgccc tgaaaaagac gtagacggtt
    16441 ttcatccgat gaatttaggc agactccttg cgggtaaacc ggacaagatt ccttgtactc
    16501 catacggcat catgaaaatg tttgaagctt attctatcga tccggcgggt aaacgagcag
    16561 tagttatcgg acgaagcaat atcgtcggta aaccgatggc tcagctactc ttgatggcag
    16621 atgcaactgt tactgttgct cattctaaaa ccgaaaatct ggctgaactg gcacaagaag
    16681 cagatatttt agtcgtagca ataggacgtg gacattttgt cacaaaagaa ttcgtaaaac
    16741 caggtgctgt agtgatagat gtagggatga atcgtgatgc taacggtaag ctgatcggtg
    16801 atgtgaaatt tgatgaagtg gagcctattg caggctggat cactcctgtg ccaaagggtg
    16861 tagggccaat gactatcacg atgctgatgt atcaaaccgt aaaatctgcc gaagcttttg
    16921 gagcaggtga atagatggca caagaatatt taactgtcac tacattaacg aagtatttga
    16981 aaagaaaatt tgatgccgat ccttatttag aacgcgtata tttaacaggc gaaatatcga
    17041 atttccgttt acgtgccaat gcccatcaat atttcagctt aaaagatgac catgcaaaaa
    17101 tatctgctat tatgttcaaa ggagcattcc aaaaattacg attccagcca aaagaaggaa
    17161 tgaaagtcct tgtcgttggg cgtatttcat tatatgaagc gggaggttct tatcaaatct
    17221 atgtagagca catggagcct gatggaatag gtgctttata tcaagaattg gaagaacgaa
    17281 aagaaaagct gatgaaggaa ggcctttttc aagggcctaa aaaaatgctg ccacgtttcc
    17341 caaagcgaat cgcggtcctt accagtccaa gcggagcggt tatccgcgat atcatcacga
    17401 cggtgaaaag aagatatcca attgtccaat tggttttgtt cccaacagtt gtacaaggaa
    17461 accaagcagc ggacaatgtt gtcaggaata tccagagagt agaagagatg ggcaatttcg
    17521 atacaatgat catcggtcgt gggggtggtt cgattgagga tttatggccg tttaatgaag
    17581 agcgggttgc tcgggcgatt tatcaagcta atacgccggt gatttcatct gtaggacatg
    17641 aaacagatac cacgatcgct gatttagtag cagatgttcg ggctgccact ccaacagcag
    17701 ctgcagaatt agctgttcct gttttgtcag aagagttgat gcgcattcaa gaacggcaag
    17761 ctagattgca gcaggcgttt gtacgccaga tccagcgcaa acaagaacgg ttcgagcgtg
    17821 ctcgacgttc ctatgtattt cggcagcctg aaagattgta tgaaggtcag accatcaaat
    17881 tagaccagtt gaaacagcga ttatatcaag ctacacagca aatttatcat gcgaaagaaa
    17941 aacaaacgat cgcattaagc caccaattgg aacaagtggc tccgctttat cgagttaaag
    18001 cagctcgcca agaaactgct tatctagaaa aacgcttaga agagaaaatc caacaatata
    18061 tgaaacaaca gcagcagcgt tttgctcaag ctgttcaatc tcttgatttg ttgagtccat
    18121 tgaaaatcat gggaagaggc tacagctata cgacgttaaa tgatcaagta gtaaaaagtg
    18181 tcaccgagat tcatcaaggg gatttgctga cgatccatta tcaagatgga atcgttaaag
    18241 gacaagtaca agagatagac aaaggagaat aaaaatatgg aaaatccaac ttttgaagag
    18301 tcactgcaag aattagaaaa aattgtcatg cagttagaac aaggagacgt tccattagaa
    18361 tcagctctag attcgtttaa acgaggaatg gaattaagca aacattgcca agatacatta
    18421 gctaaagcag aagagacctt gacgaaaatg atgacagaat caaatgaaga agttgatttt
    18481 gacggaaacg aggaaagctg agatgagatt aactgacttt tcagctattc atctacctgc
    18541 tgtagaaaaa gaaatcttgt cttttttaga tgagcataca acggatagag gactctatga
    18601 tgcgatgagt tattcagtga aagcaggcgg gaaaagaata cgtcccttat tacttttaac
    18661 ggccgttgct tcctttgatg agcctattga tgttcccgtc tatcaagtag cagctgcttt
    18721 agaaatggtg catacgtatt ccttgatcca cgatgatttg cctgcgatgg acaatgatga
    18781 tcttcgtaga ggaaaaccta ccaatcataa agtattcggc gaagcattag ctatattggc
    18841 cggagatggt ttgcttacag gagcattcca gctaatcagt atggcacact taggtaattc
    18901 tcctaagctt ctgctgcttc agcaattagc tgtttgtgcg ggatcacaag gtatggttgc
    18961 tgggcaagct gcagatatcg agggcgaatc gaaaaagctt tctttagaag aattagcatt
    19021 tattcatgaa agaaagacag ggcatttgat tcgttatgcc ttgctggctg gtggcatatt
    19081 agcgaagcaa cctgaagaaa tcttattgct acttcaacga ttagccgaac atttaggttt
    19141 agcttttcag ataagagatg atctactcga tgtgattggt acaacgaaaa cattagggaa
    19201 aacagctggt aaagatgaac gtatggaaaa aaatacttac ccgcgtttat taggcctaga
    19261 aaagacacgt gaggcgttgg agattgagct gttatccgcc aacaagatca tagacaaatt
    19321 agaagaggac gttttttctt ttgatggcgg gctgatgcgc cagatgatca agcaatttga
    19381 tgtggagaaa tgagggaaag aatggaaaaa gaacgcgtag atgtattagc ggtcaaacaa
    19441 ggattatttg aaacaagaga acaagccaaa cgttcagtga tggctggttt gatttacaac
    19501 gaaaaaaacg agcggtttga taaaccggga gaaaaaattc cagtgagtag tgaattaaga
    19561 gtcaaaggta aaaaacttcc ttatgtttct cgagggggat taaaattaga aaaagcactc
    19621 aaagcatttg atctatctgt tgaaggaaaa acattgttag atattggtgc atccactggc
    19681 ggatttactg atgcagcttt gcaaaatggc gcaaaaatga gttatgcttt agacgttggc
    19741 tacaatcagc tagcttggaa aataagacaa gatcctagag tcgtcgtaat ggaaagagtg
    19801 aattttcgtt atgcaaaacc cgaagacttt accttaggaa ttccagaagt tgctgttatc
    19861 gatgtttctt ttatttcact aaaactgatg ctgcctccat tacatgctat tttaaaagaa
    19921 gagggagaag tgattgcttt aatcaaaccc caattcgaag caggcagaga agctgtaggg
    19981 aaaaacggaa ttgttcgtga ccctcagacc catcaaaaag ttttagaaga cattttgtca
    20041 tttgcggcac aaggtggata tgatgtgctg gaattaagtt attctccgat cacgggggga
    20101 gaaggaaata tcgagttctt ggctcacttg aggaaagtac ccgaaagcgg aacaatcaat
    20161 tcagctatta atatggcaga agtggtttca aatgcacacg aacagtttga ccataaataa
    20221 attgtaactg tgaaaatcaa gtgagaagta tgcggattct tctttattat accaaggaaa
    20281 taagctatca ttgattaaga agaatatgct aaggagcgaa gacgtaatga gaaaaaaaga
    20341 tcggcatcgt ttgattactc gattgttgag tgagcaagat atccgaaagc aggaagaatt
    20401 tgttgaaatc ctgaaaagca aaggaatatc tgttactcaa gctacgattt ccagagatat
    20461 aaaggaactg aaactgatca aggtaccggc ttcagatggc ggctaccgtt atagcatacc
    20521 agctgaaaca aacgaagatg taaatactaa attggaaaaa ctattaaaag acgctttcgt
    20581 ggcagttgac caaatggaaa aatttgttat tttgaaaaca ttgccgggaa atgcttcggc
    20641 agcagcaaat ttgattgata aacgttacaa gaaagaatta ttttcgatta tcaatgatga
    20701 cgataatgtt ttgatgatta cacgaactga agaccaggca gttttattga agaaagactt
    20761 cctgcattac ctttaagttg aggagcgaat gtgatgctac aagaaatcag tattactaac
    20821 tttgcgatta ttccggaatt gcggctttct tttcacgaag gaatgaccgc gctgaccgga
    20881 gagactggtg cgggtaaatc cattattatc gatgctttag gtctgttagc aggaggaaga
    20941 ggttcaagcg attatatacg tcaaggagca gaaaaatgtg tgcttgaagg gttgtttgaa
    21001 ctaccaaaac aagaaggatt ctctgaactg atggttgaat tagggatcga aacagatgag
    21061 gacaatctga ttgttcgacg ggatatgtcc ttaactggaa aaaatgtttg tcgagtgaat
    21121 ggtcatatta tcacattagc taatctgaga agaatcggca gttatttagt agatatccaa
    21181 ggacaaaacg aacatcagga actgctccag cctgaatcac atcttgcttt attggatcgt
    21241 tttggagatg tagctttcca acagaagaaa aagagttatc agcaagaata tatctcttat
    21301 cgtgagctgg aaaaaagggt acgaaaagtg cagcaaaatg aaaaatccta tgttcagaga
    21361 atagatatgc tccatttcca gcaagaagaa atagctgctg ctgatttgca agtcggagaa
    21421 gaagagaaat taaaagaaga aagagaaaaa cttagcaatt atcaaaaaat tgctgatgga
    21481 ttagctgcag gttacggagc actgacgaat aatgaacaaa acagtgtaga tggtgttggc
    21541 ttggccgttt cagaactgca agggatcgcc catctagatg tggagtatga agcaatttat
    21601 gaaaatatcc aaagtgctta ctatttgctg caagatgcaa tcggcgatat gagtcgtcag
    21661 attgatttat tggagttaga tgagagtcgt ttagaagaag ttacgcaacg tttagaatta
    21721 atccgacagt tgaaacgtaa atacggcgaa tccattgagt caatattggc ttactatgac
    21781 gaaatcaccg aagagctagc ttcttctgat ttttcagaag gtcaattgga caaaatgaaa
    21841 gaggaactag agcaaaaaga gcttctactt cagcaacaag ccgcagatct tcatgaagcg
    21901 cgtaaaaaaa tcgcaaaaga attggaaaaa tccattctgc atgaattgaa aagtctgtat
    21961 atggaaaaca ctgagtttga ggtacgtttc ttaaaagaag aaaatagaca attgaatcat
    22021 gatggatttg accaaatcga attttacatt acgaccaatc caggagagcc actgaagcct
    22081 ttggtgaaag tcgcttctgg tggcgaactg tctcggatgt tgcttgcttt gaaaactatt
    22141 ttttcttctg agcaaggtgt gacaagtatt atttttgatg aagtggatac aggtgtgagc
    22201 ggaagagttg cacaggccat cgcagataaa atttctcaaa tatcgaaata ctctcaagtt
    22261 ttgtgcatca ctcatctgcc tcaagttgct gcagtcgccg actatcaata ttatattgtg
    22321 aaagcagtga ttgatggacg tacgcaaact tctgtttcag agttgaaaac aaaagaaaga
    22381 gaagaagaaa ttgcacgtat gctggctgga agtgagatta caaaactcac actcgaacat
    22441 gcaaaagaac tattgaaact cgcaaaaagg acctgagcca cctcgcagtg gttcaggtcc
    22501 ttttcagcag ctgatagcta tatt
//
LOCUS       DADXTH010000051        22445 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.051, whole genome shotgun sequence.
ACCESSION   DADXTH010000051 DADXTH010000000
VERSION     DADXTH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.051"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..787)
                     /locus_tag="IWI97_001812"
     CDS             complement(62..787)
                     /locus_tag="IWI97_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBC2463309.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(1148..1218)
                     /locus_tag="IWI97_001813"
     tRNA            complement(1148..1218)
                     /locus_tag="IWI97_001813"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1184..1186),aa:Gly,seq:ccc)
     gene            1335..2087
                     /locus_tag="IWI97_001814"
     CDS             1335..2087
                     /locus_tag="IWI97_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBC2463310.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2103..2582
                     /locus_tag="IWI97_001815"
     CDS             2103..2582
                     /locus_tag="IWI97_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBC2463311.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(2833..4413)
                     /locus_tag="IWI97_001816"
     CDS             complement(2833..4413)
                     /locus_tag="IWI97_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBC2463312.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(4406..5149)
                     /locus_tag="IWI97_001817"
     CDS             complement(4406..5149)
                     /locus_tag="IWI97_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463313.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(5325..6101)
                     /locus_tag="IWI97_001818"
     CDS             complement(5325..6101)
                     /locus_tag="IWI97_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2463314.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(6349..7185)
                     /locus_tag="IWI97_001819"
     CDS             complement(6349..7185)
                     /locus_tag="IWI97_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBC2463315.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(7196..8125)
                     /locus_tag="IWI97_001820"
     CDS             complement(7196..8125)
                     /locus_tag="IWI97_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBC2463316.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            8317..8670
                     /locus_tag="IWI97_001821"
     CDS             8317..8670
                     /locus_tag="IWI97_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBC2463317.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(8744..9400)
                     /locus_tag="IWI97_001822"
     CDS             complement(8744..9400)
                     /locus_tag="IWI97_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBC2463318.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            9626..11560
                     /locus_tag="IWI97_001823"
     CDS             9626..11560
                     /locus_tag="IWI97_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBC2463319.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(11646..12869)
                     /locus_tag="IWI97_001824"
     CDS             complement(11646..12869)
                     /locus_tag="IWI97_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBC2463320.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(12863..14761)
                     /locus_tag="IWI97_001825"
     CDS             complement(12863..14761)
                     /locus_tag="IWI97_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBC2463321.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(14755..15873)
                     /locus_tag="IWI97_001826"
     CDS             complement(14755..15873)
                     /locus_tag="IWI97_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBC2463322.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(15956..16789)
                     /locus_tag="IWI97_001827"
     CDS             complement(15956..16789)
                     /locus_tag="IWI97_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2463323.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(16782..17699)
                     /locus_tag="IWI97_001828"
     CDS             complement(16782..17699)
                     /locus_tag="IWI97_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2463324.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(17722..17901)
                     /locus_tag="IWI97_001829"
     CDS             complement(17722..17901)
                     /locus_tag="IWI97_001829"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463325.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(17926..19050)
                     /locus_tag="IWI97_001830"
     CDS             complement(17926..19050)
                     /locus_tag="IWI97_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBC2463326.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            19233..20141
                     /locus_tag="IWI97_001831"
     CDS             19233..20141
                     /locus_tag="IWI97_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2463327.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            20282..21895
                     /locus_tag="IWI97_001832"
     CDS             20282..21895
                     /locus_tag="IWI97_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBC2463328.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7055 a         4347 c         3761 g         7282 t
ORIGIN      
        1 ttgaatgtat gtttttacac aattttttca taatattcat aaaaaattcg aatagctgct
       61 actaattttt tgtttttgtt gcttgtcctg tctcataatt gatcgtgtag ccatcttgaa
      121 cattgtagat aaagacattg aattcaattt ttttgtcttc aatgctttct gcttccaatt
      181 gcactcccct ggctaccagt tcattcccct caaaatgagg agtgacacgg tagcgtacat
      241 gatgatttgt ttctttgata tatgccgcaa tatcattttc gtgagcaagc atgtatggag
      301 cattcaacga acgtgtccct gtcatcaagt ttttaatgtt gttattttga ccagtcagct
      361 ggaaaccaat taaatggctg cgattatata accattgccc gtcacttaat ttcttttgtt
      421 tccaacctgt cgggtcaata tacaaaggct ctctcttttc agtagggaac aaatcttttc
      481 caagcatagc attggcttgt cctactctgt tcaatttatc aatatttgaa tagctttccc
      541 atgttccttt ggaaaggcta agatcctctt ttgtaaattc cggctgattc ccattgattt
      601 ctatttcttg atgttttcca tcatattctg gcacatccgc tacttgagaa gcttgtggtg
      661 tggaatcaaa aatagttgta atctgctgaa agatatctga atctttttgt gtggtggaaa
      721 caccgaatgc ccctgcaatc acagcagcga cgatccccac gatcgccttg gttgtttttt
      781 tgctcatttc tttactcctt tactaacagc tccctaaatc ttaacatatg ttcccctata
      841 aataaatact agaaagaaat agaaacagca aaaaataaaa cctgttttaa gatggcttaa
      901 aaatacaaaa aagagacgga ttcctcgtct ctaatcagtc accactctat ctgtaaggta
      961 tttattatta ttcaaaaatt ttcttcatac tcaagacaat acactgattt cacaaccgct
     1021 tggtaaacgg tgcgcaaaag cttgcatctg cggtaataag tccatccaag ttaaaaacat
     1081 aagaagttgt ttttgcttgg ctgttcttat tacttgttgc agagcagctt tttcacaacc
     1141 tattttaagc gggtgacggg aatcgaaccc gcgacatcag cttgggaagc tgggatttta
     1201 ccactaaact acacccgcaa tacaaaaaca gtatacgcct cttttattcc ttcctcaagt
     1261 ttttttattc tttacgcttc tggtatgttt tgtagtaaaa taaaactaat caatcaatta
     1321 ttggagaaga tgctatgacg ggaaaagaat tagatctgtt gctggacact tgtttactcg
     1381 ctgggaaaat catgatggaa agcaatgcgg agatgtatcg ggtagaagat acgatgagtc
     1441 gaattgcttt agcttctgga aatttccgtt tggtcagcta tgtcactcaa accggtctct
     1501 ttattggtct tgatcgtacc tcgaccatcc gaatggaaca aattaccaac cgttcgatta
     1561 atttagaaaa agtagtaaaa gtaaatgatc tttcacgtaa atatgttgct tcagagctaa
     1621 cattagaaga actttactgc gaactgcaag caatcgaccg agaccgttct ttctttcctg
     1681 tctggttgca aatcgttagc gcggcagtga tcagtggaac aattatggtg ttatttggcg
     1741 gaaagatgac cgatttacct gtgactttag tcattggagg attgggatac gcagcttatc
     1801 tatatagttt gaaatttttt agaatcaaat ttttatcgga atttttatct tctttattga
     1861 ttggcatagc cgctatctac agcgtacgtt ttggcttagg aagcaatcag gatctaatca
     1921 ttatcggatg tgtgatgcct ttagttcctg gagttcaaat tacgaacgct atccgtgatt
     1981 tgctagctgg acattatgtc tcaggagttt ctcgcggaac agaagcgatg atgactgcta
     2041 cgatgatcgg ttttgcgatt gcttttattt tccagctttt ttattaggaa aggaacacaa
     2101 gtatggggac gatatttatt cagttttctt ttagtttttt agcgactgcg gcatttgccg
     2161 ttattacaaa tgtacctagg cgttcattga tttgttgtgg tctttctggt gcttttggct
     2221 ggatgatcta ctggatctgt gttcaaatag gcggatctgc tgcttttggt tcattgcttg
     2281 gttctttagg agtagcattc atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa
     2341 cgattttcaa tattcccggg atggttcctc tagtccctgg tggtttagct tatcaagcgg
     2401 ttcgaaatct cgttactgaa tcctatcgag aagcggctca ttatgcggtc caagcaatta
     2461 tgattgctgg agcaatcgct ttaggtcttg tattatcaga agtctttaac cataacatcc
     2521 gtaatttcaa agtaaagcgt gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat
     2581 aaacgaagtg taacaaagaa aaggttgtga aagagacgtt cagccttgag cgatgacaaa
     2641 atatctcaca aacattgcga caagtaaccg caggagcaat aagaagtaat tatgagtatt
     2701 aaaaaaagtt ttgtaaaaat agcttctcac atttttacat aaaattgact taatactgag
     2761 aaactgactt ttgaacaccc tctatctgta aaaaaggggc tgcgacatga tttttgtcac
     2821 ggcccctctt ctttattcaa attttttcca aaacgttcgt tgataataaa cgatccatag
     2881 agcggaaagt attagaacta aaccgatcac aggaatattc actaaccaaa atggataaaa
     2941 caaagccaac attccgtata agacgataag aataatactg atgatcaatg aacccatcat
     3001 agttgcgact aaacccaaac ttcctgaacc tctgttaaat aattggttaa tattggtcca
     3061 gttcagcagc agcaaccgat aatctctagc gaaataacgt agtgacaata agtagcagcc
     3121 gaataaagaa ccaaatagca agctagggat aaaaaacaaa ggcagctgaa aagacagtcc
     3181 gcttaataat gcgatcccac cagttaaaaa actctgcaat atccatccga atctgaactt
     3241 ttcttttaaa tattggttca tagagattgg caaagaacgg ataaacagaa aattttcttg
     3301 atccaaagaa ataagattgc tgataaaaga agtctgatta actgttaaga aagccaatgc
     3361 gatccctacc aggaaaacaa caccgattaa cttattatct aaatcagtta attttatttc
     3421 gcctcctagg gcaaaagcaa gaataaaaat gattggagtt aacaaggaag aagaaagaac
     3481 ttgcataatc aaattaggtt cttttactaa ttgagcattg taactaaata aaagctgatg
     3541 cacattttgg ttctttttat gggttctttt tattttgaac tcactgtttg aatcagtcag
     3601 ttgctctgta agctttggta ctatcagctg ttttacagca aggaaaaatc ccagagccgt
     3661 gatagctagt attccccaac ccaataaacc ttgttgagaa agtggctgac tggcagcatc
     3721 atagaatggc aataatatcg aaatcggatg ccgatctgtt aactcatttt caatagtccc
     3781 cgtttgatga ttcatccaca tgattccagc aaatgcgatc agcatcgagc tgccaagcaa
     3841 gatactagaa actgtttttc tatgtttttt aaacactgct gttcgagcca aaccaaaaac
     3901 aatcaaacta gccagacaaa aaagaataat caaaaataga gaaaacaaaa ggattgagat
     3961 aatgatagta agcaaaatcc ctagatcagc tcgccatccg gttaaaataa acatgactag
     4021 aagcggaaaa acaaaaggaa tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac
     4081 ctgttcttgt ccaaaaggca gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat
     4141 attataaatg actgatatac tttgagttaa acctaacata ccaaaaagtg ccacgtaata
     4201 agtaaagaaa cctggcaatt taacaaagtt catagcaaac atagcaaatc cgtaaatcag
     4261 taaaaaaatc agccctgaaa aaagattttg agacaaaaga tagatagtta atcgctttcc
     4321 cttttttcct ttctctcttg ctttattcgt gagctgtgga ttaacatata aaagattaac
     4381 tcgaattaat tctaataatt gtcgcttata catacggtcc atctcctgtc gcttgccttc
     4441 cagccatttt caaataaatc ttttctagcg tttcatcagg tgagccattc aataattcac
     4501 taactgcccc ctgataaatc aattcacctt tcttcaaaat agccagttta tcgcataact
     4561 gttgtgcggt atccaataca tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag
     4621 ccttcatcat ttctttcaaa tcaaatgctg cttgaggatc aagtccttgc aaaggttcat
     4681 ccaataccca aatatctgga tctggcagca acgtgccgat caaaatcgtt ttttgacgca
     4741 ttccatgtga aaaacttgcg attgtttcat tttgatgact atacatatca aaaagcgctg
     4801 tcaattctgc taaacgcttt tctttttttt gtgtgtctaa ttcataggca gctgaaatca
     4861 agtcccaata ttctccagcc gtcaattgta aaaaaatatc tggggaatca ggcacataac
     4921 cgattttttg tttgatactc aatcgatttt ccgtcaattt cattccatct actaaaatcg
     4981 ttcccgatga tggttcaata atactaacta aacttttaat cgtagttgat tttccggcac
     5041 cattatgtcc taaaaatcca aaaatttccc cttgtcttat cgagagagac aagtctttta
     5101 atgcttgttt tttcccgtaa cttttactta cattgatgaa ttcaatcatt tctgtactcc
     5161 ttttgtttgt tattactttt tattgtaccc ttcgttattg gtggagtaaa tgtcaaaaag
     5221 agtgcataac aaaaaaggat agaaacgact aggctgctac ttgggttgtt ctcttctaca
     5281 ggaaaacact cttctaacag gtaagatatg agtctttgtt ttctttaaaa tgattcattt
     5341 tctatctttt cttgcataaa attctttagt ttatcaatac ttctctttcc catctctagt
     5401 tgttctccat ttgcaaatgt tactatttcg tttactctgc taatagatgt aatgttttca
     5461 gtattaatga tataagcttt taaatcttta cataaatact catattcgag tgatttttta
     5521 tattttttaa ttgtgccgtt tacgagaatc tcttcatctt tcaatactag gcctatttta
     5581 tttttataac ctggcatgct cattagataa aggatttcat cgtaatggat atagtgatcg
     5641 ctattaccat aacggacagt tgtccacctt tttttcgtag catgttgaac aggatagttt
     5701 tctttttctt tcaataagta actttggatg ctttttttat ttttttcata agtgaattct
     5761 tttatcaaat aaccactagg cgtaatcttt cgattaataa cagttattgc tttattcgga
     5821 tgagacgtta gataaacaat tttacacttt tcattctttt ctctaagcat ttcgcctagt
     5881 tcgataccgc tataataggc gttcaaatcg atgtctagga aaaaaatatc tgaggaatag
     5941 atttcattat ttttaatgaa ttcaacaaat tgtataacgt tcaatccctg aacgatctca
     6001 aatttccaag gacttaataa aattctggat tcttccaaaa tttctactag cattttttca
     6061 aaaaccaaac tatcatccaa aataaatata cgcgttacca taaaaagtac ctccactcga
     6121 tattttacta tcaaaatcat ttcatacaac ttttcaacat acatgttagg aataaattgt
     6181 aattctcttc acaatctttt ccaacacctt tgaaaccgat atcaaatact ttcaggatgc
     6241 tgcttctaaa ataaatatca aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga
     6301 cgtccttgcg tgtcagctgt ctgagctatg tttatttatt ttgttctttt aaaaagaacg
     6361 atacacatac tgatttttcg gagcagagac tttctcagcg tttgtaacaa ctacagatgg
     6421 gatttcacgc ttgaatggtt gataatagtc tgattggatg gttccttcta ttttatacca
     6481 ttcatcattt ttaaccgtta ctccttccgg catatgcgtc aacaaaccaa agactcctga
     6541 atctgccaca caatggataa tgccgaatcg gaacaagaag atatctgtat tgctatcgtt
     6601 cggtgtttgg taaacaaatc cttcatagct gatttttttg ccaatgaatt cgctagggta
     6661 attataaatt agctccataa cttctaaata gttttcatct gtgatcttga tagtttgctt
     6721 atccccatac ttttctttca actcattcat ttggtctaag tagtctgatt tgttgaaata
     6781 aatactggta tccggcttta agtattgtgt ctgcatctcc ggatccccta ctgattcttt
     6841 acttactggg aaattaaatc ctttggcttc tacgatcgta gtgtcgagac taactgttgg
     6901 aaacaacgtc cccacgatta ctggcaaagc tagcagtaca taagcgattc cacgttgata
     6961 aggttttgac aacccatggt catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt
     7021 tttgacccac aaatacagct gaactaaagc caaaagagca gataaaatca ttgaaagaat
     7081 agctaagtag cgataatgga cattgatata ttgatcgagt ttgccagaaa cttgcaagta
     7141 catcatcaat tcgaaatacc cagataaaat caaaaaccgc agcatatttt ccctcctaaa
     7201 taaataatgt gaatccaata acgctaaggg caatcagtcc gatcagcgat aaaataaagc
     7261 gtcctgtaaa atatcgtttc atcatcagta gatttttgat atcgaccatt gggccaaaga
     7321 ccaaaaaggc agctacaggt gctgtgccga acagacttaa caaagaagat cctataaacg
     7381 catctgcttc tgagcaaaga gacatcgtaa aggccaataa cagcatgatg ataatggcta
     7441 aaatctttgt tgagccgagc gttagcataa ttctagttgg taaataagtc tgcatcgcag
     7501 ctgcgattaa tccgccaatg atcaaataac gacctgtatc aaaaaattcg tcgattgaat
     7561 gtgtcaacac atgcccagtt tgatggatca gctgattttt tggttcattc ttctcacttt
     7621 gttcttcttc atggacatga tgatggtgat tttctgctac tttagacgtg attttttctt
     7681 tcaatacagc gtctttttga aaataggcaa gccagacacc aatcacaagg gcaacaaaaa
     7741 agctgcctat cacccgccag cttgccatct gccaagaatt gccaaatgcg ataaaagtgg
     7801 aaaaaatcac gattggattg atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa
     7861 ctggtacgcc ttttttaaca aattgatgaa cgatcggtac gatgccgcat tcacatgaag
     7921 gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa gatacgattt tcaggaagcc
     7981 atctcttcac acgttctggg gtcaaaaata cttgtaaggc gcctgagata agacagccta
     8041 ataatacgaa aggtaatgct tcaataacga tcgataagaa aatcgtactc atctgcaaaa
     8101 ccgaatttgg aataaaagaa aacatgagtt tcctcctttt tatttctagt tcattcgctt
     8161 tgcaaatata cgcttttatt caaaaaaaac aagttgctct gtaaattttt acacaactct
     8221 tattttttac caaagcactt caattgactt tttctgttat tcccattaaa ttaaggtctc
     8281 atacatatca cttctatcat aaaagggaga ataaaaatga aacaaaccac taacttgact
     8341 gaagttcaag acatactgca acagtcatcc gttgccgtca tttatctttc gatgcctaat
     8401 tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac ttctcacaca ttatgatatt
     8461 ccggcacttc atttggatgc atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg
     8521 actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag tctttcggca agcacggttc
     8581 atcgacttta aaaaaatccg ttatctacta gatgaactga cagcagaaga tgattccctt
     8641 tcttatgaag aaatcttccg aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa
     8701 caagtgaaag ttttgccttc atttgttcaa tcgcttttta tttttattct gctgtatttt
     8761 cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca
     8821 aatcaacatt ttggatcaat acatctgacg gagcattcaa tcgagcagcg gtaaagttca
     8881 agattccttt gattcctgct tccgttaact gattgactac ttcttgtgct ctttcagcgg
     8941 gtatcgttaa aattgctact tcgatttgtt ggatcttgat ttgttctgac atatcttcca
     9001 ttggataaac ggggataccg tctacgatgc gcccaacgat ttcctcattc acatcaaaag
     9061 cacaactgat acgtatgcta ttactttgat ggaatttgta ttttagtaaa gcacttccca
     9121 agttccctac accgatcagc gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa
     9181 aaaagtgcat aagatcttct acatcataac catacccacg ttttcctaat tcgccaaaat
     9241 aagaaaaatc tcgacggatc gttgcactat ctacttgaac ggcttcactt aattcagttg
     9301 aagacacttt tttctttcca gcattattta aaatccttaa atagcgataa tacaaaggca
     9361 gtcttttagc tgtcgccttt ggaataatat gatctttcac gaaatgacct ccacgtattg
     9421 tctaattaca tctttgattt ctccatcata gcagtattta taggaaaaaa tcaattccat
     9481 tgctcagata ttgtgaaatt ttattcttta ttcatttatt tatcatttct gcaaatctcg
     9541 cagtataatg gaatttatgg tagactacta attgaaaaat agttttttat ttgaggagaa
     9601 ttcacaaagt agaaaagagg attccatgat attattacaa gccaatcaga ttgctcgcca
     9661 ttttggtgca gaagtcttat ttgaaaacat acatttagaa atcagtacag gcgctcgtgt
     9721 cgctttagtt ggcagaaatg gcgctggaaa atcgacttta ttaaagatca ttgctggaat
     9781 tgaagcacct gatgaaggaa agatcgctaa aaataaaaca gcgactctcg gatacctcgc
     9841 tcaggacaca ggacttgatt ccaatgaaac agtttggaac gagatgctga aagcttttga
     9901 acaagttaga caaatggaag agcggatgcg cgagctagag attttcatta gcgaaggtac
     9961 gccagacaca cctgcttata gttcttattt aaaagaatac gaccggctgc aacacgattt
    10021 ttcagatcga aacggctatg gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa
    10081 attcgattct tcattttatg atcagaaaat cgatacttta tcaggcggac agcgcacacg
    10141 tttagcttta gctcgcatgc ttttgcaaca ccctgacatt ttgattctag atgaaccaac
    10201 gaaccattta gatatcgaga cactttcctg gcttgagaat tatctgcaaa gctatactgg
    10261 tgcattattg attgtttccc atgaccgtta ttttttagat aaagtagtca atgaagtcta
    10321 tgaattaagc agaagaaaaa tgcgccatta caaaggtaac tattcgaaat atctagattt
    10381 gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca
    10441 taaattagaa gacttcgttg ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca
    10501 aagccgcaga aagacattag aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa
    10561 acaagcaaac tttctattca gtatagaaaa gccttctgga aatgtagtct tgcaaatcga
    10621 agatgccgct atcggatatg ataatgtcac cttatctgaa ccaatcaatc tagatatccg
    10681 tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa
    10741 atcaatcatt caccagatcc cttttatccg tggaaaagaa acgctaggga caaatgtcac
    10801 catcggctat tacgatcaag gtcaagctga tctgcataca aacaagacga tcctagcaga
    10861 attgtgggat gagcatccta ccactccaga aaaagagatc cgaagtgctt taggtgcttt
    10921 cttattttct ggcgaagatg tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc
    10981 acgggttgct ttagccaagt tagcgatgaa caaagaaaat tttttgattt tggatgaacc
    11041 aacaaaccat ttagatattg acaacaaaga agtattagaa aatgcattga tcgattatga
    11101 cggcacactg ctttttgttt cccatgaccg atatttcatc aatcggatcg cgacaaaagt
    11161 cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga gactatgatt attatttaga
    11221 gaaaaagcag gaagaagaag aaatcgccga attgttggca caagatgagc cagaatctgc
    11281 tcctgctccc acgaacaaat ctgattatta ccaaagcaaa gaacagcaaa agcttatccg
    11341 tagccttcaa cgaaaaatta ctcaggttga agaagaaatg gcgcggatcg atgaatggat
    11401 cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa
    11461 tgagctgaac caagatttag aagctgctag acaagatcaa gaaacaaaat tgacggaatg
    11521 ggaagaacta agcttggaat tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa
    11581 ccattcatgc tgttttggtt tttctttttt tattttatca aacaaaaatt ccatatttac
    11641 ctttttcacc aataagaaga ccaattacta gtcattctca ctaaagcttc taaataatag
    11701 taatcacccc aaatacaagg tgtatttacc ccattatttt tgggcttatt ataaacagac
    11761 tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata
    11821 attttccaaa cagcagactg gtataagttc ttttctgttt tgtctaatag acctgattct
    11881 attaattcta ataatccaca aaccatgatc accatactag atgtatcttt ttcttctctg
    11941 ctagaatcat caaagatcaa atcccaataa caaatggtat cttctggcaa ccgattcata
    12001 aaataattgg ctaatatttt gcttaaatca aaatattttt gttctttcaa ataacgataa
    12061 cttaatgcaa aaccgtaaat cccccatgct tggcctctag cccaacagga attatcacta
    12121 taaccttgat gcgtacttcc atatcttggt tttccatctt tgacatccat ataaaatgta
    12181 tgataagttg aaccatcttc tcttactaaa taatctgctg cattttgaat atgagcctga
    12241 gcagcttttt tatatttttg atttccagtt tcttcactcg cccaatacaa taatggaaga
    12301 ttcatgcaac aatccatgat cattctacct tgttgtgaag gatcactagg atctccccaa
    12361 gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt
    12421 gaagcttcct tcatttttcg atttcccgtt attttataat ctgctacggt tgataaagaa
    12481 tacaaaaaac caagatcgtg atgattagtg ataatttgac agtcaatcct gtggataaaa
    12541 tctatatctg ctttttcagc tgtttgttga aataaaggat cacccgtctt ctcaaataac
    12601 aaccaattaa ttcctgtcca aaatccttct gtccaaccaa tattgtctct taatggataa
    12661 cgtcctttta taggacactc gcttgggaat tgatactgac tgttactgat acgtttgatt
    12721 aacctctccg tgtgtctgcg acaacgctcg atctcattat tccattcttt ttccattaat
    12781 tctttataac attcatttat ccatttttct ggatgtatta tctcttcaaa catcacttta
    12841 tcaagcattt ccccaccagc ctttagcata attttagtct ttttttattg tttttatttt
    12901 ggatagttag cccggttgtc ccatcaaaat caattttggg acgattgttc catacttcta
    12961 aactataatc ccgatcttga tgagcaacca ctcctgttcc gagccaatgg gttccttttc
    13021 caaatgtacc ctctaatcca ggaataaaag tgttttccca tgtcatcaag tttgtattgg
    13081 gcaaacccat aatagctgtg gattttcgtt taatagtatc gtcacttaaa cagataatac
    13141 ctgaaaagcc agcttcactc accctatagt tttccttgtc ttcttctatg attattcctt
    13201 ctttctcagg agaacattca atagcaaacc ctgtctcata tagaacatat tctgtatcta
    13261 attctacttt gtgtacgcga atctgccagc cttcaaaagg aataagccaa gttctgattt
    13321 ttgcctgtaa agtaggttcc catgtagagg caagtacctc tttcttacat acaatgtctc
    13381 gtacattatt tcgaacgatg aactgtccat aggttttgat gctctcattc ttaaaactgg
    13441 tcgtgatata tgtgtccgct cgctgaatcc ctagcattga atccattgcc atttctgctt
    13501 tcaattgatt cgctcgcggt atcgaaaaac caaattgact agaataagca aattttaaat
    13561 atttttcatt ggtcaatgta tggaaatttg gccctaattg tcccgcattc aaaaaaaatg
    13621 tatgtccatt atcatgttgt atgagcaaat tggcttctga aagaagttta gttccttcac
    13681 gaataggcat aggcatttct ttagccgtcc aatactcatg gtcttcaggt agcgccaaca
    13741 ataaaaaaat cttattcagc cataatggag ataacgtact attatagggt tcagtcaatg
    13801 tcaattgagg gtaggtataa ccaatattca ggataccttt ttcatcaaat atttcttttt
    13861 gcatccacca tcttatattc cgattgacta accccttgat ttcacctaat ttaaaaggca
    13921 aaagatcggc ccacaacata gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc
    13981 ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa
    14041 aatcaatagc tcggttctta tatatctggc taatgggctt ttctggttct agtacgctat
    14101 aaatcaaacc ataataatga aatgcaaaag gattataata atcttggcga cctcttgaac
    14161 tgtctcgata ccagccattt tgaagataac aattttctat attatcaaat gcttctttca
    14221 gtgctgattc tttaactgga taaccaaatt tttggataat tgttccgaca attatcttaa
    14281 aaaattgcca attaccatct gcacattcat attcaaaaat ttgcgagaac caatgaataa
    14341 tctggctttt ttctcctgta tctaattttt tccataataa ttgttcatga tgatataacg
    14401 ctaataacat tgcgggcatt tctaccatac gctggtctct ttcatgtatt tctccccaat
    14461 aatcgggatg ggaaggatct gttccagctt ttattcctga tagaattaat ttcaaccatc
    14521 gttcatctaa ttggtcaatt gcgggccccg ctccccataa aacccgagag aatccttcca
    14581 ttcctgcaat tttatttcca tatcctaccc cttgatgtat ataatcaatt tgtgcgccac
    14641 ttgctcgata aaacggctgg agtggttgat ttatcttttt aaataagtca aagtaatcct
    14701 gtctggtcct taatagtggt actggatgtt tcattgtatt ttctcctctt tttactacca
    14761 aatagctaat tccgactgtt ctaatcgaag taagccttca ataaaaaaat aatcagaata
    14821 gatcagtggc acattataag tcagttctct atggtatgct tctgctccgc ctccaatgat
    14881 cccatcttca tccatattcc aatttgcata gtgcttttct gtcttttgga gtatattgat
    14941 agcagcttca tgatacattt ttgatcgtac agaggaaaca tatcctgcta attccagaag
    15001 accgcatgca gcgcatactc cagcactggt atcaatattc gaatctttta cagcaaaatc
    15061 aatgagagga atataaccag ttttttcgat tttctgaata aaatttactg ctatcttttc
    15121 tgctatagct agatagtacg gattttttgt gtaacgataa cttaacgcga atccatataa
    15181 agcccatgca tttcctctgc tccaacaaga attctcattg tatccttgcc cgccaagtaa
    15241 ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata tgcccggttg atccatcttt
    15301 cctcactaga tacttagctg ttgtatctgc atgctgccga gcaattttag agaacctcgg
    15361 atcaccagtt tccctcgaag cccaatacag taatggtaaa ttcatcatac tatcaataat
    15421 catccaacca ggtttatggt cattccatgc tactaaatag ttccctgaaa tattatagcg
    15481 tccagcaagt accgtagctg catgtaaccc tagttgtcga ctatgtttgt tttgagactg
    15541 ttgataatgt gcaatagccg atggtaagaa taaaaaacct acatcatgat gaacgtctga
    15601 aaaatggtac agggcagatt ctaataattg ttcttgttgt attgctgcct ctttgaactt
    15661 ttctcgtttt gtcttgtgaa agagctgcca taaaatacca ctccaaaaac cattggtcca
    15721 ccagtcgatt cctctgtcct ttattacgtc tgtatatttt ccgttgtaag aaatatatgg
    15781 aattttattc cccattctgt ctaattccgc atctatcttt tcagaagcta cattcaaaat
    15841 ttttcttgac cattccttcg tctgttcatt catggatatc ccgccattct tttaattttt
    15901 atgaatgtgg agttgctcct agaacgaact agatggttaa cattcgttat tttttttact
    15961 tatttgcagc tccaatgtca ttctccttaa atttagactg cgcccacaca taaatcaaga
    16021 ccattgggat cactgaagtt gctaaaatta gcataattgc gccataatta attggctcac
    16081 ctgtactaga aaccgtcata tttttgataa ttaagggaac cgtatattta tctttatctg
    16141 aaagcatgac taatggaaat agatattgat tccaacttcc cataaagata agtaaaccac
    16201 tagtaataat cgtaggtgat aaaatcggta cagcgatttt cgtaaaaatc acccactcat
    16261 tggcaccatc gattctggca gcctccatca gtgctgtagg aaattgactg ctatattgtc
    16321 gcatcataaa cactgaagtt gtactggcaa tcgaagggat gatcacacct gcataagtat
    16381 tcgataatcc taaacgactc ataatgtaaa ataaaggaat caatgtggta aaaaaaggca
    16441 ccatcctaga tagcataact gtattgaaca acagtttatt ccccataaaa cgatactttg
    16501 ctaatgcata acccgccatt gtagaactgg ctgtagctaa aactgtactg attacagcaa
    16561 taacaatcga attccaaata atcaagggat aattatatct cgccatgatg gcttgccagt
    16621 tttttaatag catatcccca aacttgaaat tgattccgcc acttaaaatt tcttgattac
    16681 tttgcgttga agctacaatc atatataaat atggcatcaa aaatattaag gcaccaaaca
    16741 caataatgac gtacgtaaaa attctgcttt gtatcttttg tttagtcatt tttatcacct
    16801 ccgaatttaa attgaatagc cgataatata acagatacaa acaccatcgt ccaggcaata
    16861 gcagaggctt ttccaaaatc aaccatttca aaacttgttt tatataaatt gactcctacg
    16921 accaaagctg aattttctgg tccattggaa gtcccaaaga atatatttgg gatattgaaa
    16981 ttacccaagc cattgatagt ggctaaaact gtggccatta aaatgattgg ctttagcatg
    17041 gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc catctatttc agcactttcg
    17101 tatatctctt tagaaatccc ttgaagacca gctaaaaata aaatagtgtt atatcctaca
    17161 ctaagccaaa tcatcgtgat gatcattgat attcgtatcc aaaaaggatt tgttaaccac
    17221 tgtacaccgt taattcctag cgcattcaaa aatccattga ataacccagt cggattgaaa
    17281 aaagtcatga atacggaagc tacagccacc gttgatgtaa tggttggaat ataatagaca
    17341 gttctaaaaa atccttttcc ttttaactgt gctgaattca ataaaacagc aaagtaaata
    17401 gctaatagca tcgttattgg aattgatcct gccatgataa tcaaaacatt cgtgtaagat
    17461 ttaataaaca atggatctcg gaagatagct agataattgt caaatccaac aaattcaaat
    17521 gagtagccaa ttttcataaa actaaaagca aacgagataa tgatgggaat caaaatcgtc
    17581 acaataaaga aaataacaaa aggtgtgacc aatccataag cacttttttt atgagacgta
    17641 tctgttgctt ttttcttgct gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc
    17701 gttaagtcct cctaatattt tttaatcacc agttaatttt acgccgtact tatgagcaaa
    17761 attttctgct tcttttagat atgaattttc aaaatcacct gtttgccaat agttatatgc
    17821 agcggtagtc aaataagacg tagcatctga ataagtggct ggtaaaacag tatttgatgt
    17881 attttcattg attttattca atagttcata gtatttttgt cctccttagt agtcaaactt
    17941 tttctcagct gcttctgttt gataggccaa tgagttagat gtagccacgc ccagctccag
    18001 tgcgttggct aagcattctt catctgtcag cataaatgta agaaattgtt gtgccacttg
    18061 tggatttttg gcatctttag ggacataaaa acttgatcct cctgatactg caccttttcc
    18121 tggattttct tttgaaaaag gaatcagtgg agccatgcgc catttgtcag atgcatcagg
    18181 aaaattcaaa gacatattcg tagcatacca gccgccagca acaaacattg cagattcttg
    18241 aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca atatctgagt cgatcatttc
    18301 tttgattaac tttgcagcat gtttcgcttc tttagtattc agacggatat ttccttcttc
    18361 atcaagcaaa gattgattct gctgggctag catgttgata aacaattctt ggtccccaga
    18421 ttctggcaag gcaattccaa aactaccagt tttttctttt aatgcttttg ccacctggat
    18481 atattcttcc caagaactga tttcttcaaa atctacacct atttcagcaa atttttttgg
    18541 ttgataataa caaagtgctg gggtaaaatc actagcccaa ggaataatcg tttcattact
    18601 cgtttgttga cgcaaaatat tcaatttttg atcataaaac tgatcgccat atttgtcata
    18661 aaatccatat tcatcagcac tataaaaagc atcttgaaat ttttccagca cacctgctgc
    18721 tgaagtgtct gccatggaga taatatccgg catatcttcg tgtgttacca attttggggt
    18781 gatcatttgt actgttgctg ttcccgaagc tacattcaca atttttaagt caatatttgc
    18841 tccagttttg cggttataga tttttgcagc attttcaaca tagtttgtca gtttgccgtt
    18901 tgcgacatat acttctaatg tagtggcttt ttttatcatt ggatctgcga tgtttgctgt
    18961 tacttcttcg ttagtttttc cacatgcact taagctgaat aatagggtaa tgtttagcaa
    19021 agctatcacc aatttgtatc tttttttcat gtctatccct tctttctttt caagttctta
    19081 tataaaacgc tttcacttat gtatattaac ggaattagta acacaaatat ttatataaaa
    19141 aaatatttta cttgtcgttt tggcttttca tttttgttat aatagtcatt aattataaat
    19201 agaaagcttt ttatataaaa agagaaagga aagtggttct tttgttaaca aacaaccaat
    19261 cctccaatct tgtcaaaaaa acactaacct attccataga tacccatctt catttatctg
    19321 ctgctcccta ttttcgacta ctaataaatg aaagcgctaa tttatatgct gaatttgcac
    19381 ataaatctat agaactagat aatccttccc ttttattcat tgataaacag caagaagtcc
    19441 aaatcttttc tactcaaaag gaaacacaaa cttttatcat tatccagttt tattttgatg
    19501 agcaattatt agcacatatt acgggacaat ctttttctat agatcaagaa ataacatcta
    19561 tcattacttt tctgaaaaat gaacagttca aaattgatta tctaaaacat agtaagccag
    19621 ctatttccta tgatcaggaa atcaattctt ttttcatact atgccaacag atgagcattc
    19681 atttgctttc cttattagtt acttattgtc tgcaaaaaat tcaattgtct tccttaccaa
    19741 ggcattatca acaggaactt gctttattga caataccttc aaatgagaaa gtcattccta
    19801 aaaaaaagca tattctttat tcagatgaat tagttgatcc aatcatacga tatttagaag
    19861 aacatctttc agaaaatttt ttgcttgaac aattagccca aatttttttt atcagtccag
    19921 ccagcttaaa aaaacatttt agaaaagtga atggaaaaag tgttattagc tattttaaag
    19981 aactaaaaat ggaaaaagca aaacgtctcg tgctagaaaa tgaattaacc tatacagaaa
    20041 ttgcagatat attaggattt cattcgatcc atcatttttc ttcagctttt aaaaaatata
    20101 cagggttatc cccttcaaag tattatctat ccttaaaata gaaaaaaatt tttaggtttt
    20161 atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa ttttccggca
    20221 caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag gagatgtttt
    20281 tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca ctcgtttccg
    20341 tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata tttcttatgc
    20401 aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact cgctgatcga
    20461 acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta aacacgaagt
    20521 aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca caaatgaaga
    20581 aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag attatccgga
    20641 tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga cggatgcgga
    20701 tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc ttggtgcagg
    20761 tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg tgactttaat
    20821 cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt tagtttatga
    20881 caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg agatccgtga
    20941 taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata tgctaatttt
    21001 tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac aattatcaga
    21061 tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca tctatgcgat
    21121 tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa gtatgctttc
    21181 cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta cgcctatgcg
    21241 ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag caagttatgc
    21301 gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa ctgtttttat
    21361 cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc taaagctgat
    21421 ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa aaggtgtcga
    21481 taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct ttgatttgcc
    21541 tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt tgaatatagc
    21601 tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag caagtgatat
    21661 acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta ataaagcacc
    21721 ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag atcgaatcaa
    21781 tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga atacttatac
    21841 ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag aataaaaaaa
    21901 cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta aaaatgaaaa
    21961 agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg aagaaattgt
    22021 gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat tgcgcaattt
    22081 aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga caaatcatag
    22141 tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat attaatctat
    22201 cagcactagt acctaaacta gcatcccttt cgaatatttt ttcttatata ataaagtttg
    22261 ctttcactcc tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac taggaactga
    22321 ttggtttaga acgacttttc tattgtaatc atatttttcg ccatcctcgt tgtcttctaa
    22381 tccttcaatg aatgatccct aatgtgtgta ttaaataatt taattttttt gttcctattt
    22441 attta
//
LOCUS       DADXTH010000052        21972 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.052, whole genome shotgun sequence.
ACCESSION   DADXTH010000052 DADXTH010000000
VERSION     DADXTH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.052"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(48..527)
                     /locus_tag="IWI97_001833"
     CDS             complement(48..527)
                     /locus_tag="IWI97_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBC2463329.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            886..1668
                     /gene="rpsB"
                     /locus_tag="IWI97_001834"
     CDS             886..1668
                     /gene="rpsB"
                     /locus_tag="IWI97_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBC2463330.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1767..2648
                     /locus_tag="IWI97_001835"
     CDS             1767..2648
                     /locus_tag="IWI97_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBC2463331.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2784..3506
                     /locus_tag="IWI97_001836"
     CDS             2784..3506
                     /locus_tag="IWI97_001836"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBC2463332.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3509..4066
                     /gene="frr"
                     /locus_tag="IWI97_001837"
     CDS             3509..4066
                     /gene="frr"
                     /locus_tag="IWI97_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBC2463333.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            4261..5073
                     /locus_tag="IWI97_001838"
     CDS             4261..5073
                     /locus_tag="IWI97_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBC2463334.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            5070..5870
                     /locus_tag="IWI97_001839"
     CDS             5070..5870
                     /locus_tag="IWI97_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBC2463335.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSTVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            6031..7299
                     /gene="rseP"
                     /locus_tag="IWI97_001840"
     CDS             6031..7299
                     /gene="rseP"
                     /locus_tag="IWI97_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBC2463336.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            7367..9076
                     /locus_tag="IWI97_001841"
     CDS             7367..9076
                     /locus_tag="IWI97_001841"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBC2463337.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            9288..13640
                     /locus_tag="IWI97_001842"
     CDS             9288..13640
                     /locus_tag="IWI97_001842"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBC2463338.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            13784..14257
                     /gene="rimP"
                     /locus_tag="IWI97_001843"
     CDS             13784..14257
                     /gene="rimP"
                     /locus_tag="IWI97_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBC2463339.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            14281..15456
                     /gene="nusA"
                     /locus_tag="IWI97_001844"
     CDS             14281..15456
                     /gene="nusA"
                     /locus_tag="IWI97_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBC2463340.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            15478..15771
                     /locus_tag="IWI97_001845"
     CDS             15478..15771
                     /locus_tag="IWI97_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBC2463341.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            15768..16079
                     /locus_tag="IWI97_001846"
     CDS             15768..16079
                     /locus_tag="IWI97_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBC2463342.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            16092..18398
                     /gene="infB"
                     /locus_tag="IWI97_001847"
     CDS             16092..18398
                     /gene="infB"
                     /locus_tag="IWI97_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBC2463343.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            18425..18772
                     /gene="rbfA"
                     /locus_tag="IWI97_001848"
     CDS             18425..18772
                     /gene="rbfA"
                     /locus_tag="IWI97_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBC2463344.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(18880..19101)
                     /locus_tag="IWI97_001849"
     CDS             complement(18880..19101)
                     /locus_tag="IWI97_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2463345.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            19549..21912
                     /locus_tag="IWI97_001850"
     CDS             19549..21912
                     /locus_tag="IWI97_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBC2463346.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINSVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         7569 a         3882 c         4811 g         5710 t
ORIGIN      
        1 ttgaaaataa aaaaacacgc attccatatt aggaatacgt gctctgatta aaaaagtgat
       61 acttcagttg gatggtgaag aaattcttct actcgttttg cgtcctcctc attttccgag
      121 atgaccatga tcgtatcgaa tccagcaatt gtactgacaa tagagggagg tttgatctca
      181 tctaaaacag atgcaaataa atgagcattg tctggcaagg tattgacgat cgtcaaaaag
      241 tggactcgtt cgactttcaa tacaatgtct tcaatcattt gaaccagccg atcctcttcc
      301 tcagtcctag tctcaggagt tcctttttcg cgaaacaata cgaagcggga aacgccattt
      361 tcatcaggtg ccttcacgat ttttagatca cggatatctc gagaaatcgt tgcctgcgtg
      421 gcggtaacgc cataatgccc aagtttgttt aatagttctt cttgcgtacg gatcgggaat
      481 tcttcaatga cttgtttaat cagcaaatgt ctttgagctt tgcgcatttt ctttctcctt
      541 tacgaaagat ttcacatgta tcataacaaa aaaaagaagg attgcccaat aatattcgtc
      601 ttttatacct aaaattattt tatggaaaaa ggataataaa gaaacaaaga gaaatctact
      661 gtttttcttg aattcttttt atctttcagg taaactattg aatgttgagc agaaaagtat
      721 atttgctctt gcgatacgga tcttttgtct gtataatagc tgagggtgct ttatgcatca
      781 gcaaatccac attcttattg gattgtcggt aggtgcttga aaaagtttgc cggcaaggta
      841 gaagataaga agaggaaaaa taaaaccaaa tcggaggaaa caaacatggc agtaatttct
      901 atgaaacaat tactagaagc cggcgtacac tttggtcacc aaactcgtcg ctggaatcca
      961 aaaatgaaga aatatatctt cacagaaaga aacggaatct acatcattga cttgcaaaaa
     1021 acagttaaat tagtagatgc agcttatgat tacatgaaga gcgttgctga agaaggcggc
     1081 gtagcattat tcgtaggtac aaaaaaacaa gcacaagaag caatcaaaga agaagcaact
     1141 cgtgcaggac aatacttcgt taaccatcgt tggttaggcg gaacattgac aaactgggat
     1201 actatccaaa aacgtatcag ccgtttgaaa caaatcaatg caatggaaga agatggaact
     1261 tttgaagttc tacctaaaaa agaagttgct ggtttgaaca aagaacgtga acgtcttgaa
     1321 aaattcttag gcggtatcgc tgatatgcca agaatcccag atgtaatgta catcgttgac
     1381 ccacgtaaag aacgtattgc tgttcaagaa gcacacaaat taaatatccc aatcgtagct
     1441 atggttgata caaactgcga tccagatgaa atcgacgtag taatcccttc aaacgacgat
     1501 gcaattcgtg ccgttaaatt gattacttca aaaatggctg atgctttcat cgaaggaaac
     1561 caaggtgaag atcaagtagt tgaagaagat ttcacagaag aaaacacagc aacatcaatc
     1621 gaagaaatcg tagatgttgt tgaaggcgac aattcttcag cagaataatt ttgaatgatg
     1681 gagctgtttc aaaggctggg cgacaagcct cactctcctt tgaagcagct ttttttaaaa
     1741 agaaaaacac acaggaggag ttaaacatgg cagacgttac agctaaaatg gtaaaagaac
     1801 tacgcgaaat gactggcgta ggaatgatgg atgcgaaaaa agcattagtt gaagtagaag
     1861 gcgacatgga aaaagcagtt gatcttttac gtgaaaaagg aatggcaaag gctgcgaaga
     1921 aaaatgaccg cattgcagct gaaggattgg cttcagttgc tgtgaaaggt aacactgcag
     1981 caatcgttga agtaaactca gaaacagact ttgtttctaa aaacgaaatg ttccaagatt
     2041 tagtaaaaga aattgcagaa cttgttgctg aaaacaaacc agcagacatg gaagctgcaa
     2101 tgaaaatcaa aacagataaa ggtacaattg aatctgactt gatcgaagca actcaagtta
     2161 tcggagaaaa aatcagcttc cgtcgctttg aagttgtaga aaaagacgac aatgctgcat
     2221 ttggtggata cttgcatatg ggcggacgta ttgccgtatt gactgttcta gatggaacaa
     2281 ctgacgaaac agttgcaaga gacgtagcta tgcacgtagc agctatcaat cctcgttatg
     2341 taaacgaatc acaaattcca gaagctgaat tagaacatga aaaaactgtt cttacagaac
     2401 aagcattgaa cgaaggcaaa ccagcgaata tcgttgaaaa aatggtagaa ggacgcttga
     2461 agaaattcaa agcagaaatc gctttggtgg accaaccatt cgttaaagat cctgatatga
     2521 ctgttgaaaa atacgttgca tcaaaaggcg caactgttaa aacattcgta cgtttcgaag
     2581 ttggcgaagg tatcgaaaaa cgcgaagata actttgtaga agaagttatg agccaagtga
     2641 aaaaataatt ctgttgaata acagacggga acgcacggtt tgtgcgctcc ctttttttaa
     2701 gcaaaacaag aaaaaccagt cttagagtgt cgaaatcatc taagtatggt aaaatggcaa
     2761 tagaaaatat tggaggaatg aacatggtca caccgaaata tcagcgtgta gtcttaaaat
     2821 tgagtggaga agctttagct ggggatgcag gatttggaat caaaccacca gtaatcaaag
     2881 aaatcatcca agaaatcaaa gaagttcacg agctgggcgt tgaaatggca atcgttgtgg
     2941 gtggcggaaa tatctggcgc ggacaaatcg gagcacaaat gggcatggaa cgtgctcaag
     3001 ctgattacat gggtatgtta gctacagtta tgaatgcctt agctttacag gatacgttgg
     3061 aaaacgtggg tgtaccgaca cgcgtgcaaa cttcgattga aatgcgtcaa atcgcagagc
     3121 catatattcg ccgtaaagca gaacgtcatt tagaaaaagg acggatcgtt atctttgcag
     3181 gcggtaccgg caacccgtac ttctcgacag acacaacagc agcattacga gcggcagaaa
     3241 tcggcgcaga tgttatcctg atggctaaaa acaatgtaga cggtgtttat tctgctgatc
     3301 cgaaagtaga tgcaaatgca gtaaaatttg aagaactgac tcatctggaa gttattgcca
     3361 aaggattaca agtcatggat tcaactgcaa gttcactaag catggataat gatattccac
     3421 tgcttgtctt caatttaaat gaacacggaa atattcgccg tgcaatctta ggcgaaaata
     3481 tcggaacaac tgtaaggggg aaataaaaat ggcagatgca attatgacag aagcaaaaga
     3541 aaaaatgaaa aaagcagcac aaaatcttca acgtgaactt gggcaaatcc gcgcaggccg
     3601 tgcgaacgct agccttttgg accgtattac tgtgaactac tacggtgcgc caacaccatt
     3661 gaaccaaatg gcatcaatcc aaattccaga agcacgtgtt ttaatgatca caccttttga
     3721 caagagcatt cttcaagatg ttgaaaaagc tattatggca agtgatatcg gaatcagtcc
     3781 aaccaatgat ggaaatgtca ttcgcttagt tatcccgcaa ctgacagaag aacgcagaaa
     3841 agaattagct aaagatgtga aaaaagaagc agaaaatgca aaaatcgcga ttcgtaacat
     3901 ccgtcgtgat gcgatcgatg aatataaaaa acaacaaaaa aatggtgata ttaccgaaga
     3961 tgatttgcgc ggattagaaa aagacgtaca aacattgaca gataacagca ttaaagaagt
     4021 agataagatc gctgctgata aagaaaaaga attattggaa gtttaataaa atcataaaaa
     4081 cagaggggcg gatcattccg cttcctctgt ttttatttat atttctttta tttaaaaatt
     4141 aaaaaaagaa aaaattattc agtttttaaa taaaaaaaca atgatttcat aggaaaaaca
     4201 gttagatatt gctataatac ttaagatagt gggaatcttt taagtatagg aggaaaaaga
     4261 atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt
     4321 aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg
     4381 gcgcaaaacc gtcgtttgcc acgagttgct ggccataaag aaggcatgga aacagtaaag
     4441 aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg
     4501 actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac
     4561 ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt
     4621 tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca
     4681 aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc
     4741 gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa
     4801 atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa
     4861 gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg
     4921 cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg
     4981 catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa
     5041 aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg
     5101 cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag
     5161 caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa
     5221 gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat
     5281 ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt atagttatga
     5341 ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc
     5401 ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag
     5461 aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat
     5521 atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa
     5581 cgattgaagg tgcgctcggt ggtatcggta gtactgttgt agtcgcattt ctttatttat
     5641 tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga
     5701 ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt
     5761 atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg
     5821 acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc
     5881 tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact
     5941 attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa
     6001 cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt
     6061 tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga
     6121 atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa
     6181 gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc
     6241 aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct
     6301 gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg
     6361 atggaattga gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga
     6421 gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac
     6481 ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag
     6541 cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc
     6601 atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag
     6661 atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt
     6721 gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca
     6781 ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca
     6841 ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg
     6901 aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg
     6961 gaaattttca aagccttagg atcactattt acaggtttca gtttagataa attaggcgga
     7021 cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt
     7081 agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc
     7141 ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt
     7201 caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg
     7261 ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta
     7321 taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa
     7381 aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat
     7441 cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt
     7501 agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg
     7561 agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg
     7621 ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat
     7681 ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata
     7741 taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc
     7801 tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc
     7861 aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt
     7921 caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa
     7981 agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac
     8041 tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc
     8101 gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga
     8161 agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc
     8221 ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact
     8281 gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata
     8341 tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata
     8401 tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt
     8461 ccactataaa aatgtgaatc caaaacgcga tttcactgtg ctttcttatg aagatcttcg
     8521 ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg
     8581 aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac
     8641 tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt
     8701 aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc
     8761 aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca
     8821 aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt
     8881 tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc
     8941 aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa
     9001 aactggcgaa atggttgaag tacgtaaaga agaattggcg aatactttac caatcctctt
     9061 gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt
     9121 tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat
     9181 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
     9241 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
     9301 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
     9361 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
     9421 aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
     9481 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
     9541 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
     9601 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
     9661 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
     9721 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
     9781 tggatgagca gcaagcggaa cgtgtattga aactttttga agaacgcaaa caagaacagg
     9841 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
     9901 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca gctaggacga aatattccag
     9961 ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg
    10021 aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt
    10081 tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag
    10141 atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc
    10201 aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac
    10261 atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata
    10321 gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggaa
    10381 aatggggaca ccgagctatc gccattacag accatggtgg tgcgcaagcc tttccggaag
    10441 cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg
    10501 tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat
    10561 acgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc
    10621 tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc
    10681 caggacatcc attgtcacga acaacggtag atctgactgg tatcacggat gaaatggttc
    10741 gagggtcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa
    10801 ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt
    10861 atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct agatatctgt
    10921 atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaagtttggt gttagcttgg
    10981 aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg
    11041 tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg
    11101 gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc
    11161 aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac
    11221 gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct
    11281 cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa
    11341 gaaaccgagc aaaattctat gattatattg aagttatgcc aaaagcggtc tatgcgcctt
    11401 tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg
    11461 tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga
    11521 acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt
    11581 tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat
    11641 ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg
    11701 cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag
    11761 gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc
    11821 cgttacctga gatcattcaa aaacgattag aaaaagagtt gaattctatt aatggtaatg
    11881 gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct
    11941 atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca
    12001 ctgaagttaa tccattagcc cctcattatt attgtccaga gtgccaatat tccgagtttt
    12061 ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg
    12121 gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg
    12181 gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata
    12241 actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag
    12301 tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt
    12361 ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg
    12421 gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc
    12481 cgatccaata tccagcagac gatcaaaatt cagaatggaa aacgactcat tttgatttcc
    12541 actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta
    12601 ttcggatgct tcaagattta tcagggatcg acccacaaac gatccctacg gatgacccgg
    12661 aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt
    12721 ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga gggatgttag
    12781 aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta
    12841 cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg
    12901 aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg
    12961 ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg
    13021 aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg
    13081 attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga
    13141 tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact
    13201 tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga
    13261 aacaagcaat gaaagaaatc acggacaagg gattagatgc ttccgtaaaa gaaaagaatc
    13321 aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga
    13381 ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac
    13441 catttagagc tgtcccaagt ttgggaacca acgtggcgaa acaaatcgtt gaagcaagaa
    13501 aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat
    13561 tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat
    13621 cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta
    13681 tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct
    13741 ttttaatgga attacgcact taatttttaa ggaggcgaaa cttttgagta gcgtcgttga
    13801 aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac ttgtcgaagt
    13861 cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca aagaaggcgg
    13921 tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc
    13981 agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg gagcagagcg
    14041 gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc atgtctcctt
    14101 ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat ttgatgctga
    14161 acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa
    14221 aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa tcagaaaaaa
    14281 atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa
    14341 gaaatcgtga tcgatgcttt agaagcagca ttggtttctg catacaaacg tcactatggc
    14401 caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg
    14461 gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca
    14521 cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa
    14581 gattttggac ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa
    14641 gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga
    14701 atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt
    14761 ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt
    14821 tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat
    14881 ccagacctat tgcgtcgctt atttgaacaa gaagtaccag aagtctatga tggaatcgtt
    14941 gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat
    15001 gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca
    15061 atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct
    15121 gtattcatcg caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca
    15181 aatccaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa
    15241 cgcggacaaa atgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca
    15301 gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg
    15361 catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc
    15421 aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg
    15481 aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa
    15541 gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa
    15601 atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat
    15661 aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt
    15721 cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta
    15781 atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg
    15841 gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa
    15901 ccgatgcaag tgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc
    15961 cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg
    16021 tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg
    16081 aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta
    16141 gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg
    16201 cgattactag cagtgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac
    16261 aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta
    16321 aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg
    16381 gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg
    16441 gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta
    16501 acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc
    16561 gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac
    16621 cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag
    16681 atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg
    16741 gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg
    16801 actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct
    16861 tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagta gtaacgatta
    16921 tgggacacgt agaccacggg aaaacaacct tattggatac attgcgccat tcacgtgtaa
    16981 caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg
    17041 gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac
    17101 gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc
    17161 cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg
    17221 taaataaaat tgataaacca ggtgcaaatc ctcagcatgt tatgcaagaa ttaagtgagt
    17281 atgaattaat tcctgaagca tggggtggag aaacgatttt tgttgagatc tcagcgaaat
    17341 tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt
    17401 taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag
    17461 gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcacgtt ggtgacccaa
    17521 tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg
    17581 ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag
    17641 caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac
    17701 gcgcaaaacg tgcattattg gaaaaccgtg ctgccacaag ccgtgtgact ttggacaact
    17761 tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg
    17821 tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc
    17881 gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag
    17941 cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc
    18001 aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag
    18061 aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc
    18121 aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg
    18181 taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa
    18241 tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa
    18301 tgggttatga atgtggtgcg atgattgaaa aattcaatga catcaaagta gatgacgtaa
    18361 ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat
    18421 cattatggct aactaccgtg atcgccgagt cggtcaagaa atcttaaaag aagtcaatga
    18481 tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa
    18541 agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc
    18601 agataagaaa aaagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt
    18661 agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt
    18721 tgtctatggg aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaaa
    18781 tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt aagacgtatt
    18841 acctaagagt tccatgactt tagtctggta gcgtgtcagc taaccaaaaa agagaaaaag
    18901 aaaagtaaaa aattcgactt tcttttttct atagtatata aatttttgca tatagataaa
    18961 gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag tcaggtatag
    19021 ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg tcggtccgtt
    19081 gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc cgcttcgatt
    19141 tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc tgggatacca
    19201 attcacatat tgacaaagat aacagagact tttacataaa acggatcaat caggaaagga
    19261 attgctcgat ccagccccag ttttccaaca atgtgttaac tggcctagcg tcattcagca
    19321 aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata acgacaagtt
    19381 ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt
    19441 tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag gtttcaattg
    19501 ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat ggattattct
    19561 agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta tctttctgtt
    19621 gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc agaagatgtc
    19681 aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat ttatgtccat
    19741 ttgaaccgag tcatcaataa atatgatttg aacatgtttt atatcgaagg gccaggacac
    19801 ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga gatctatcca
    19861 gaagtaacgg aagatgagac aggaatgcaa aaattattca agcgattttc atttcctgga
    19921 ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg cggagagctt
    19981 ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt aatctctgct
    20041 gtcgtgattg gtgatggtga agcagaaacc ggtcctttag ctggaagctg gttctcaaat
    20101 gtctttatca attcagtgat agatggagct gttttaccga tccttcattt aaatggtgca
    20161 aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc aaattacttt
    20221 aacggacttg gctgggagcc tttctttatt gaaggaaatg atccagaaaa attaaatcct
    20281 gttatggctg aaaaaatgga tcaagcaatc gagaaaatca aatccatcca aaaagaagct
    20341 cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca
    20401 ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg aaccttccga
    20461 gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga tgctttactc
    20521 cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg tattttagaa
    20581 gaaatcagag aaattgcgcc tactggtgac caaagaatgg caaaaaatcc tatcaccaat
    20641 ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac tttgcaattt
    20701 gaaaaaccag gctccgttaa agcagaagat atgaccgaac ttggtaaatt cgttcgcgag
    20761 atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac aaaatcaaac
    20821 cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa
    20881 aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa
    20941 gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt tgcaagttac
    21001 gaatcattct tgcgagtggt tgactctatg ttgactcagc acttcaaatg gatacggaaa
    21061 tcacatgacc tttcttggcg aaaagattat ccttcgctga atctcattgc aagctcaaca
    21121 gtcttccagc aagatcacaa tggatatagc caccaagatc caggaatatt gacacatttg
    21181 gcagaaaaga aggcggagtt catccgtgaa tatctgcctg cggatgcaaa tacattgctc
    21241 gcagtcatgg ataaagcatt ccgctcgtca gaaaaaatca atttgatcat ctcttccaaa
    21301 catccgcgtg ctcaatttta ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg
    21361 aaaatcattg attgggcttc gacagcaaaa gaggaggaac ctgaattggt cattgctgct
    21421 gcaggaacgg aatctaactt agaagcatta gcagctgtaa ctttgctgct ggaagaattc
    21481 ccaaaactaa aaattcggtt catcaatgtc gtagatttat tgaaattacg tcatccaagt
    21541 caagatccgc gtgggttgag cgatgaagaa tttgatcaat attttacaaa agacaaaccg
    21601 atcttatttg ctttccacgg ttatgaaaca ttggtccgca cgattttctt tgatcgtcat
    21661 aaccatcatt taatgattca tggctacaaa gaaaatggag atatcacgac accatttgac
    21721 atgcgtgtag taaatgaact tgatcgttat catttggcaa aagacgcagc tttaaaaatc
    21781 aaaggaagtc aagcagaaga ttttgcggaa aaaatggatc agaaattaca agaacatcaa
    21841 aactatattc gtgaaaatgg tattgatctg ccagaagtat tggattggaa atggaaaaat
    21901 cttgaccaat aaaatgataa aatcgatccc gcaaaacaga attttttaca ttgtattatt
    21961 tgtcaaatta ag
//
LOCUS       DADXTH010000053        21665 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.053, whole genome shotgun sequence.
ACCESSION   DADXTH010000053 DADXTH010000000
VERSION     DADXTH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.053"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(63..1442)
                     /gene="brnQ"
                     /locus_tag="IWI97_001851"
     CDS             complement(63..1442)
                     /gene="brnQ"
                     /locus_tag="IWI97_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBC2463347.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            2148..3119
                     /locus_tag="IWI97_001852"
     CDS             2148..3119
                     /locus_tag="IWI97_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBC2463348.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            3143..5335
                     /locus_tag="IWI97_001853"
     CDS             3143..5335
                     /locus_tag="IWI97_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBC2463349.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            5352..5828
                     /gene="ybeY"
                     /locus_tag="IWI97_001854"
     CDS             5352..5828
                     /gene="ybeY"
                     /locus_tag="IWI97_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBC2463350.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            5812..6207
                     /locus_tag="IWI97_001855"
     CDS             5812..6207
                     /locus_tag="IWI97_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBC2463351.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            6222..7121
                     /gene="era"
                     /locus_tag="IWI97_001856"
     CDS             6222..7121
                     /gene="era"
                     /locus_tag="IWI97_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBC2463352.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            7264..8076
                     /gene="recO"
                     /locus_tag="IWI97_001857"
     CDS             7264..8076
                     /gene="recO"
                     /locus_tag="IWI97_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBC2463353.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            8460..9377
                     /gene="glyQ"
                     /locus_tag="IWI97_001858"
     CDS             8460..9377
                     /gene="glyQ"
                     /locus_tag="IWI97_001858"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBC2463354.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            9379..11454
                     /locus_tag="IWI97_001859"
     CDS             9379..11454
                     /locus_tag="IWI97_001859"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBC2463355.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            11838..12617
                     /locus_tag="IWI97_001860"
     CDS             11838..12617
                     /locus_tag="IWI97_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463356.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            12658..13602
                     /locus_tag="IWI97_001861"
     CDS             12658..13602
                     /locus_tag="IWI97_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBC2463357.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            13618..14397
                     /locus_tag="IWI97_001862"
     CDS             13618..14397
                     /locus_tag="IWI97_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBC2463358.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            14410..15108
                     /locus_tag="IWI97_001863"
     CDS             14410..15108
                     /locus_tag="IWI97_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBC2463359.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            15136..15900
                     /locus_tag="IWI97_001864"
     CDS             15136..15900
                     /locus_tag="IWI97_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBC2463360.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            15913..17730
                     /locus_tag="IWI97_001865"
     CDS             15913..17730
                     /locus_tag="IWI97_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBC2463361.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            17744..18967
                     /locus_tag="IWI97_001866"
     CDS             17744..18967
                     /locus_tag="IWI97_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBC2463362.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            18981..19715
                     /locus_tag="IWI97_001867"
     CDS             18981..19715
                     /locus_tag="IWI97_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBC2463363.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
     gene            19732..20343
                     /locus_tag="IWI97_001868"
     CDS             19732..20343
                     /locus_tag="IWI97_001868"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2463364.1"
                     /translation="MVIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRS
                     YTEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNIL
                     MRLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIAL
                     ETDALNNDVVNNFYKKNEFILERKYSTNEGRIMNEYRFYMEKN"
     gene            20346..21491
                     /locus_tag="IWI97_001869"
     CDS             20346..21491
                     /locus_tag="IWI97_001869"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBC2463365.1"
                     /translation="MKLLYILNVANKVNNFSKASMMAAKQTGLEFYIAGNWKYKSDEE
                     RKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVVGRI
                     AGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQK
                     LRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELNENK
                     NHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLL
                     AAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEI
                     AGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
BASE COUNT         7493 a         3738 c         4466 g         5968 t
ORIGIN      
        1 tctttcttct agctgttttt ttcctgttta atatctctta tgttaaattg tgtttgattt
       61 ggttattctt ctggtaactc acttgcttct tgtttatatc ctgatacgcg atatacataa
      121 taaaagagca agctgccagc gtaaccaagc aatgttggga cgacccagcc aagacctaat
      181 gcagcaaaag gaacataatg atgataaaat cctagtaagc ttgctacgac agtttgctga
      241 ctcactaatg caggcgaatt tgccaacata tccaatacgg ctggaacggc agaaaagaac
      301 atcgttactt gatagacaaa tggactcttt tggaagaatt ttgctgttaa ggaaagtaag
      361 attaacgcaa gtgacaatgg atacaaaagc atcaatactg gtaaagacca ttggatgata
      421 ttatccaatc cggcattcgc aacgacaaaa gagacaaaag tcgttaaccg cagccatgtc
      481 atataactga ctttagggaa cagtttatgg aaatcttgtg caaaagaaac aacgagtccc
      541 atagcagtag tgaagacacc taatgtgacc attaccccta agaagctagc accgaagtta
      601 cctaaataaa aattagtgat ctgagaaagt gcagttccgc cattttctga taaagacatc
      661 ttattcaaac tcatcgcacc gagaaggacc agaccaaaat aaatcatgac ttctaacgca
      721 acgctcaggc taccagcttt tgctgttaat tctgtgactt ttttatcttt aaaaccaaga
      781 tcacgaactg catggacaaa agtgacactt aaagcaagta aagctactgc atcaaccgtg
      841 ttgtatcctt ctaaaacgcc atttgtcaaa gcgttcgtct gataagaatc tgcagcagat
      901 tggtttagat tccccatggg ttggaataaa gaaacgacaa aaatgatagc taataatgca
      961 agaaacaagg agtttaaata ctttccgaca tatttgacca aacgtgttgg atgtacagta
     1021 aggaaataag caagtaaaaa gaaaagagca gagaataaaa acatacctaa tgaatcatat
     1081 ttttcaggca aaaagggttt agcagccatt tcaaaagcag ttgctgctgt ccgcggtgta
     1141 ccaaagaatg gtccgatcgt taaatgaaca aggatcaaga acaaggcagc ataccatttt
     1201 ccgacaggtt tagctaagtc atacagacca tcactttttg tcactactac agctaagata
     1261 gctaataatg ggaaaagagc gcctgagatg gcaaatccta caccagcgct gatccagttt
     1321 gatccagcca tttgacctaa gtgtacagga aagatcagat tacctgaccc gaagaacatc
     1381 ccgaagataa gtgaactgac catcaataat tgcttccatg aataagtttt tgtttcttcc
     1441 ataaattttt cctccaataa ttatcatttt gagtaacaaa ataaacgttg cagaaactag
     1501 aaataccact aatatcagat tccatgaaaa ccaccacctt aaaatataaa aaaagccctt
     1561 ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc gcttaaaaaa
     1621 gcttcttcat tcacggacgc ttttcgataa agttatccgc tagcctgata atggaggcag
     1681 ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat tcgatttatt
     1741 gtcatttctc acttacatga ctcactggga ataaatcact gaatagccta ctgttttcct
     1801 cgacgctttg ctgtatattt atttgttgac gttataatac acctgttttt tttgaaatgc
     1861 aagaatattt caaatatttt gttttctttt tttgaaacaa gaaaaatacc cagagaatac
     1921 ttgctaagtt agacggaata cttgtcctct atcttatgca tagcttacat gatcagcttt
     1981 ttttattcaa acgagacctt tagagaatgg aagcggctag ttttttaacg tttcttggta
     2041 cttatggtta agcaactctt ttagaacctt ttcttatttg ctatatagtt tcttctgagt
     2101 atggtaaaat aggaaagaat taatcgaaaa aaaggggatg tctatttttg acggaagagt
     2161 ctagttcttt agagattaga ttgacggctg ctgatgatgt cagcatgctt ttaggtgcac
     2221 acgacaagca tataaaactg attgaggaaa caacggaaac aaccatccat acacgtggag
     2281 aaatcattca gatcattggt gagcaatccc agatttcctt ggctgcctca gtcattcgga
     2341 cattacagga attgatcaaa cgaggaatcc acgtaagtac gcccgatgtt gtgacggcat
     2401 tgaaaatggg gcaaaaaggt acattagatt attttgttga gatgtatgag gaagagatcg
     2461 tcaaagaccg taatggaaaa cctatacggg taaaaaacag cggccaaaag aaatatgtcg
     2521 aatcggtagc caaacatgat atcgtatttg gcgtaggacc agccgggaca ggaaaaactt
     2581 ttcttgcagt agtattagca atcgctgcct tgaaaaaagg tgaagtgcaa aaaatcattt
     2641 tgacgcggcc tgctgtggaa gcaggtgaaa atctaggctt tcttcccggg gatctaaaag
     2701 aaaaagtgga tccttatctg cggcctgttt atgatgcttt ataccaaatt tttggattgg
     2761 atcatacgaa ccgattgatg gaacgaggcg taatcgaaat cgcaccttta gcttacatgc
     2821 gggggcgaac attggatgat gcattcgtta tcttggacga agcacaaaac acgacagtcg
     2881 ctcagatgaa aatgttcttg acccgtctcg gttatcaatc aaaaatgatt gtcaatgggg
     2941 atacaagtca gatagacttg cctcgaggga cgacaagtgg tcttgttcat gcagaacgaa
     3001 cattaaagca aatcaagaaa atcgattttg ttaattttga agcaagtgac gttgttcggc
     3061 accctgtagt tgccgaaatc atcaaagctt atgaaaaggc agatctccat caagaataag
     3121 ctttgcttag gaggataagc acatgttgaa taaaacactg cagaaactac aagaaaaact
     3181 tggaaaggca gcattgcctt tcctgctgtt cttgtttttt atcattatct gcggcgttac
     3241 atttagtagt gtcaaacaaa aggccaatga tttcagagaa ggccaagtag cagaagaaag
     3301 tatccgtgcg aacaaaacga tcgagaacac ggaagaaacc gaacaaaaaa gaaaactggc
     3361 agcagaagca gtcactccag aatacactta ccaaaaagat cttgcagatg accagaacaa
     3421 tcggatcaag caactatttg aattgatcga taagacgaat gatgccatca acaaatctta
     3481 tgacgaaaag attgataagg caaaggaaga tgaaaatatc ccaaagccta ctacagaaga
     3541 gcggatcgcc gcgctgaaga aaaattttga aaatgtgaat gcagaaaatg ttgtgtttta
     3601 tcaaaaacta ccgaacaatt tttattcgac gatatttgaa atgactgaaa ctgagattca
     3661 tactgtgagg gatgagagtc tgaagctcat tgacgaacag atgagcaaac aagttcgtga
     3721 aagtgaattg gaagctttta aacaagaagc agaagagcaa atccaatatt tgaatgtcac
     3781 accagcacaa caacaaatga ttcgctactt agtggatcaa ggtatcgtag tgaatgacgt
     3841 attgaatgag aaaaagacag aagaattgaa acaatccgca cgagaagctg tacaaccagt
     3901 gatgatcttc caaggagaaa tcattgttcg tgaaggtaat caaatcgatg cttcagctat
     3961 gaaaaaattg gaattactag gactgactaa tcaaacaact tcgatttttc cgctaattgc
     4021 catggtttta gctgttttgc ttcaaatcgg cgtactgatt tataattcac ttcaattcca
     4081 aaatgcagga gaaagaacaa aatacgtgct tttctatgtg acagctatgt cagtcagcat
     4141 agtgctgatg aaatttttcc aattattcca aacggaacaa gctacttata tcccgctgtt
     4201 ttatcccgct gcttttgcac cacttgtttt aagctttttc ttgaatcgaa gagcagggat
     4261 catggctggt attttccaag cagtatccgc acttttcatt ttttatgatt cgattggcac
     4321 gaactttcta acgattatct tgatgtctta tgttttctct gggctcatgg caacgattgt
     4381 cagacgcaaa cggatctcag aacaaggatt atctgctgcc atgtgggtca ttatttttcc
     4441 tgtgatgatg gatgtcattt tgatcgtcta tcaaggaatg agctttggtg atgcaacgac
     4501 ttggttaatg ctcgtctgcg ggttcactgg tgcactcttc tcattcttgc tgacgatcgg
     4561 tctacaccca tatattgaat tgatggtcaa tgatgacagt gtaattgtat taaatgaact
     4621 cagcaatcca aatcatcctt tattgaaaca gttgctggaa gaagcgccag gtacttatca
     4681 tcatagtatg atggtagcta atctaagtgc taacgctgta gcagaaatcg gcggacgttc
     4741 tttgctaact cgagtagctt gctattatca tgatatcggc aagatcaaac atgctagctt
     4801 tttcgttgaa aacttgcctt caggagcgga aaacccgcac aatttcttat tgccagaaga
     4861 cagtaaacat attatctttg gacatgtgac agatggagcc aaaatattgg aagattataa
     4921 tatgccgcaa atggtgatcg atatttgcaa acagcatcat ggaaccacgc taatgcgtta
     4981 tttctacatc aaagcgaaag aacgcaatcc ggaagtaaca gaagagcaat tccgttatcc
     5041 tggaccaaaa ccgcaaagtc gtgaagcagg gatcgtcaat attgctgaca gttgtgaggc
     5101 tgcagtacgg gcaatggatc atccgacgag cgaaaaaatc gaaagttttg tccataatct
     5161 tatcgaagag cgtatttccg atggtcaatt agacgatagt ggactgacct tgaaagaaat
     5221 ccgaaaagtg gaaaaatcat taatcagcgg tttaagtagc accttccatt ctcggatcaa
     5281 atatcctaaa atgaaaacag aagttgagaa gataaaggaa gtacaagaag aatagaaaga
     5341 agcgatgtat aatggatatt acctttatgg atgaaacaaa ccaagtctca gaagagaaaa
     5401 tgaaagaaat cgatgacttg ctgcaatttg cagcatctta tttaaagctg ccagaggaaa
     5461 cagaaatgtc tgttactttc atggacaatg cggcaattca agtaatcaat cgtgattacc
     5521 gtggaaaaga tgcaccgaca gacgtcatca gttttgctct ggaagaagaa ggcgaagacg
     5581 aaatcccagt gatttttgaa gatggagaga atccattacc tcgtgtacta ggagacatca
     5641 tgatctctat tgaacgtgct gctgaacaag cagaagctta cggccattca tatgatcggg
     5701 aacttggctt tttagcggta catggatttt tgcatatcaa cgggtatgac catatgacac
     5761 cagaagatga gaaagaaatg tttggtttac agaaagagat cttggatgcc tatggactca
     5821 aaagataaaa acgtagagaa aaacaaacat ttcctgatgt ctgtggaatt tgctgtccaa
     5881 ggagtcaaaa ccgtttttga ggaagaacgc aatatgagga aacatgttgc gttcggtatc
     5941 cttgctttat ttatgggcgt gattttccag cttgatcgga tagagtggtt atggctgctt
     6001 ttagctgttt ttctcgtttg gatagtagag atcatcaata ctgtttttga aaatgtagtg
     6061 gacatgttta cggattttca ttttcatccg attgggaaaa aaatcaaaga tatggcagct
     6121 ggagcagttt tagtgaccgc ttgttttgct gtcatcattg ggttgatttt atttgtccct
     6181 aaaatatatc atttgttgtt taattaaaaa ggagaacgta tatgccagaa cataaatcag
     6241 ggtttgtcgc aatcgtcggc cgcccgaatg taggaaaatc cacattattg aaccgaatcg
     6301 tgggtcaaaa aattgccatt atgagtgaca aggcacagac aactagaaat aaaatccaag
     6361 gagtatatac tacaccagaa gctcagctga tttttatcga tacaccagga atccataaac
     6421 caaagcatcg tctaggagat tttatggtag aagcagctta cagtgcgttg aaagaagtgg
     6481 atgcagtctt atttatgatc agtgctgatc aaaaacgtgg ccggggagac gattttatta
     6541 ttgaacgtct aaaaaacgtg cattctcctg tttatctgat tatcaataaa atcgataaga
     6601 tccatccaga cgagttgctg ggaatcatcg aagattattc gacgcagatg ccttttgcac
     6661 aagtgattcc gatctctgct acagaaggaa acaatgtcga acgcttaatg gatgtattag
     6721 tcagtgaaat gccagagggc ccacaatatt ttccagatga tcaagtgact gatcatcctg
     6781 aatacttcat cgtttctgaa ttgatccgtg agaaagtctt gtttttaaca cgagacgaag
     6841 tgcctcattc tgtcgcagtc gttgttgatt cgatgaagcg aaacgagaat gataaaatcc
     6901 atatccaggc cacgatcatc gtcgaaagag atagccaaaa aggaatcatt atcggcaaag
     6961 gcggaaaaat gctgaagcaa gtaggaacga aagcaagaaa ggatatcgaa aatcttttag
     7021 gagacaaagt atttctagaa ctttgggtca aagtccaaaa agactggcga gacaaaaaag
     7081 tctatctgca agacttcggc taccgaaaag acgagtactg atattctgga cttcttatag
     7141 gttaggtata gaaataaact ggataaaaat ccgtacgttg agaaaaacag cggaagatcg
     7201 aaacataaat aatctggatc ggcagaatct gcttttccag atttattttt taggagggag
     7261 aatatgagcc agcttgtaga atcgaaaggc ttgattttat ctagccgtaa ttacaaagaa
     7321 aaagataaat tagtcaaaat ctttacggaa tcttctggca aaatgatgtt ttatatcaaa
     7381 ggaatccatc ggcaaaatca gccattagct ccggctttgc tcccctttac cgaagcagtc
     7441 tatatcggta attttcgaag tgaaggtttg tcgtttttaa gcagtgtcaa agacgtccat
     7501 gcgtttcgga acatccaaga agatatcttt ttgagcggtt atggtacgta tttgctaaat
     7561 ctagtagatg cagcaatcga agatcatcaa tatgacccgc atttattcca atttacgcaa
     7621 caagctctgc agcggatgga ccaaggagat gatgcagaaa tcatcacgaa tatctttgaa
     7681 gtgcaaatac tgcaacgctt cggaatcgcg ccaatttgga cgcactgtgt cgtatgcggg
     7741 gaaacaaaag gaaaatttga ctattcttcc aaatatggcg gtgtgatatg cgagaaacac
     7801 tggccaatgg acgaacaccg ttatcatgca gatcctcgcg ctgtttattt cgttcgaatg
     7861 ttttcggcta tttcttatga taagatcagt ggaatccact tgaaagaaga aacgaagcag
     7921 gcaatccgtg acctcattga ccagttgtat gatgaatatg tcggtattca tctaaaaagc
     7981 aaaaagttca ttgatcaaat gaagtcttgg gcacacgtat taaagacgcc agagaaaaat
     8041 atgcctgaaa aagagatacc tgaaaaagaa gaataaatga aagatacaac aaaaaaatca
     8101 aaactatttg acaaatacag ggacttccgt ctattattaa ccgtagatta aatagatcgt
     8161 tcactgagag gatctattat acaaaccaga aaactgcaaa atgaatacgt acattgacca
     8221 agatcagtaa aaaacacgtg gtttagcgag ttcgggagag tggaagccgg acccaaacga
     8281 tttttgaaga ggcacttgcg agtacgataa atgaagctgc cgaatcttta ttcggaagta
     8341 gggtggaacc gcgaaattat tcgtccctat gtctgtaatc agacataggg actttttgtc
     8401 gttttctttc ctgtattctt attgacagca acgcttaaaa attgaaaggg gcaaaacaga
     8461 tgagtaaaaa attgaccgtc caagatatga ttttaacatt acaaaaattt tggtcatcaa
     8521 acggctgcat gctgatgcaa gcttatgata cagaaaaagg agcaggtacg atgagcccat
     8581 atacattctt acgggcaatc ggaccggaac catggaatgc tgcttatgta gagccatccc
     8641 gccgaccagc tgacgggcgt tatggagaaa acccaaaccg tttgtatcag catcatcaat
     8701 ttcaagtcgt aatgaaacct tcacctgaaa atatccaaga gctttattta gaaagcttgc
     8761 gtcttttagg aattgatcca ttagaacacg atattcgctt tgttgaagac aactgggaga
     8821 acccatcgat gggttgtgcc ggtgtgggct gggaagtatg gcttgatggg atggagatca
     8881 cacaattcac gtacttccag caagtcggag gactaccttg taaaccagtt acttctgaga
     8941 tcacttatgg actagaacgt cttgcttctt atatccaaga agttgaaagt gtttatgatc
     9001 tggaatgggc tgatggcgtg aaatatggtg agatcttcaa acaaccagaa tatgaacatt
     9061 ctaaatattc ttttgaagta agcgaccaag acctgctact aagcaatttt gatcgttttg
     9121 aaaaagaagc taaacgctgt atcgatgaaa atcttgttca tccagcgtat gattatattt
     9181 tgaaatgtag ccatacattc aatttattag atgctcgcgg tgcagtatcc gtaacagagc
     9241 gtgcaggtta tctttcccgt atccgcaaca tggctcgtgc tgtagccaaa atctttgtgg
     9301 cagaacgcga aaaattagga ttccctttat tgaataaaaa cacagcaaat ccagaaaacg
     9361 tgaaggaggc tgaataagat ggcaaaagac ttattacttg aaatcgggct ggaagaaatg
     9421 cctgctcatg tagtcacacc aagcatgaaa caattagaag aaaaaacagc aaaattttta
     9481 gatgaaaacc aattgactta tgatacgatc gaaacgtttt ctacaccgcg ccgcttagca
     9541 atcaaagtaa caaacattcc agaacgtcaa gcagacagcg aagaagaagt caaaggacca
     9601 gccaaaaaaa ttgctttgga cgcagatgga aactggacga aagcagctga aggatttgtc
     9661 cgcggacaag gtctgacgac agatgctatc acttttagag aattaaaagg cgtggaatat
     9721 gtctatgtaa cgaaatacac tcacggcaaa gaagcaaaag aagtattgac aggtttagca
     9781 gatgtcatta aaagcctcac tttcccagta acgatgcact gggcagaata cgattttgaa
     9841 tatattcgcc ctattcactg gatcgtggca ttgttagatg acgaaataat cccgtttgag
     9901 atcttggatg tcaaaacaga cagagtttca agaggtcatc gtttcttagg cgaagatgtg
     9961 acatttacgc atgcaaaaga atacgaagaa aaattgaatg aacaatttgt tattgcttca
    10021 ccgcaaaaaa gaaaagaaat gatcatcaca caagcaaacg aattagcaga aaaaaatcac
    10081 tggatcattg atctagatga agatttacta gaagaagtca acaatctagt cgaatatcca
    10141 actgtctttt ctggcgactt cgaagaaaaa tatctatctg ttcctgaaga agtactagtc
    10201 acttctatga aagaacatca acgttatttc gaagtacgga acgatcaagg tatgttgctt
    10261 cctcatttta tttctgctcg taatggcgat cgggtaaaat tggaaaatgt ggtacgaggc
    10321 aatcaaaaag tattgactgc tcgtttagaa gatgcggaat tcttttataa tgaagacaag
    10381 aaagcttcaa tcgaccaata cgttgaaaaa ttaaaaactg ttactttcca tgaaaaaatc
    10441 ggtagcatct atgagaaaat gcaacgggta caagtcatcg ctcgcatcat aggcgaacaa
    10501 gtaggactat ctgaggaaga attgatggat ctgcaacgcg ctgctgaaat ctataaattt
    10561 gatctagtaa ccaacatggt aggagaattc cctgaactac aagggatcat gggagaaaaa
    10621 tatgctctgc ttaaaggcga gaaaccagcc gtagcaacag cgatccgtga acattacatg
    10681 cctatatcca gtgaaggaga tttacctgag actacagtag gttctgtttt agcgattgct
    10741 gataaattag atagtatctt ttctttcttt gcagttggga tgatcccaag tggctcaaac
    10801 gatccatatg ctttgcgccg tcaagcatat ggtgtcgttc gaatcatcga atcgaaaaaa
    10861 tgggcctttc ctttgtctgt tctgcaagaa acgattagtg aagttatcag taaagatacg
    10921 gatcgttttg gcattggctt gtcagcggga caacaacagg tgatcgactt tatcaaaggc
    10981 cgtctgcgcc aattattgac aacgaagaat atccgtcatg atgtcatcga agctgttttg
    11041 aatgcagaac aaaaagatct gacaaaagta tttgcagcag cacaattgtt caaacaacat
    11101 cttgcagatg aagacttcaa accttcaatg gaagcattga cacgtgtcgt gaacttagcg
    11161 aaaaaagcgc agcttgaaaa acaatcagaa gtagaccctg agctgttcga aaacgacgca
    11221 gagaaagaat tacacaaagc agtagaagca atgaaagcaa gttttgctga ccaaacgatc
    11281 cacgaaaact acgaccagct tgtcgctctt cgtccactga tcgaaaatta ttttgatcaa
    11341 acgatggtca tggctgatga accagcagta cgtaagaacc gactaacaca acttagtcaa
    11401 ataactgcca tggctttgtc attagcaagc ttggatcaaa tcatcacaaa atagtctttt
    11461 gtttgaaaaa cagagagcat gcacgaaacg tgctgtgtct ctgttttttt gttataaata
    11521 taaaagctgt tattaattct attttaataa tttttgttat tttattcttt gctagaattt
    11581 tcaaaacgct catgaaaagt gaggaaataa aaatgaaaaa gattaataat aatcttgtta
    11641 ctgttttatt tacttttaaa ttaggaattt ccctagtcaa gaaaactgtt tgtggtatga
    11701 ttatataggg aaaacctgtt tataaatatt tctatgaata tattagttta attatgcttt
    11761 taaaataaaa agcgctcatt ctgatgtgaa tgaatcacat ctttatttat ctatcatcaa
    11821 tatttaggag tgaatctatg gaaaagccgt tcgtcaagtt attggctact gtggcggtag
    11881 gcacaggcgc tattgtgatt tgcttgtttg gataccatct caataatcag cggcaacatc
    11941 atcaacggat caattacgca gaatctgcca tcaccaatca aaaagacacc gtgacctctt
    12001 tatcaaaaga agtagataag ctgtacagta caaaagaaaa aattttccta aatcctgaaa
    12061 taacagaaga aacagtttca aatttgtcac ataaactatc ttctatcaaa ctttcagcag
    12121 atgattatga catcaaggaa tcagaattac ctaaagaagc tgctgcgatc caagaagaaa
    12181 agaaagcagt cttaacacag ttagaggatg cagaatcaaa actgaagatc caaacggctg
    12241 tcaataaatt atttactaaa aacgtatcaa actggcagca agctgtggat gatgtcatca
    12301 tcaaagaaaa gttggcaagt gcagatgtcg cccatgtacg tgaaaatatg agctttttta
    12361 aggatagtgc ttggaaaacg gtcgttatgc aatatctagg attcgctgat acccaaattg
    12421 cacaagtcac gcaactagat cagctattcg atacgatgct gaaagacggt caagtaacag
    12481 ctactgctac ttacgatcaa tatttgaccg cactcagtct catcgaacag atccggaatg
    12541 aaaaaataag agccgcatac gcgtcaaaag cagaaacagt cgctcagcaa atgggctata
    12601 gcaatacgag ctattaataa gcgagaattc aagcaaggct agcaaacgga ggaaactatg
    12661 gcaaaacgaa gaagaagatc aagagggaaa aagatatttc taattatttt agcaattctt
    12721 gtcgtattag tattagcaat cgtaggagta ggagcaaaat tatatctaga tctctcacat
    12781 tctattgaaa aaacttacga atcagtggaa cgaacccaag gagaacgaac aaaaccagtt
    12841 gatctggaca aacaagaatc tttctctgtt ctattgatgg ggattgatac gggagattta
    12901 ggtcgggtcg atcaaggaag atcagatacg atgatggttg ccacagtcag tcctgaagac
    12961 aaacagacaa cgattgtcag cattcctcgt gatacgtatg tcgacatcgt tggacatgac
    13021 acaaaagaca agatcaacca cgcttacgca ttcggcggag ctgccatggc gatggatacc
    13081 gtacaaaaat atgtagacat cccaatcgat cactatgttt caatcaatat gaaaggtcta
    13141 aaagaactcg ttgatgcagt tggtggtatc gaagtcaata atgacctcac cttctcacaa
    13201 gatggctatg attttacgat cggtaaaatc acattgaacg gggatcaggc attgtcttat
    13261 tctcgtatgc gttatgaaga tccaaatggt gactacggca gacaagaacg tcaaagaaag
    13321 atcgtagaag gtgtagcgaa aaaagtgttg agtctgaacg gtgtaagcaa ctatcaatcc
    13381 gttttgaatg ctctagagtc gaatatgaaa acagacatga gctttgatga aatgaaaaag
    13441 attgctttag attatcggga tgcatttggt acgatcaagc aagatcagct gcaaggagaa
    13501 ggcttcatgc aagacaatat ctcataccaa aaagtcagcg atcaagaact tgatcgtgtg
    13561 aaaaaagaat taaaagatca aatgaatcta gagaataaat aggaaagtgg agaaaaaatg
    13621 gaagagacaa tcagcttgaa agaaattttt gaaacattac gcaaacacgt gataacaatc
    13681 ctcatctcaa tgtttgctgg cttggccatc tcaggcatcg tgacgttttt catcttgacg
    13741 ccaaaataca gctcacaagc ccaattgatc gtgacactgc cgcaatcaga tactgcaaat
    13801 gtcaatgatg tcaatacgaa cctgcaaatg atcaatacgt ataaggatat gatcgtcagt
    13861 gatctagtac taaatgaagt caaagatcgt ttagaaacag aagacaatgt aaaaatgact
    13921 gctggacaaa tcaaagatgc catctccgtc aatcagtcac aaaattcaca aatgttctcg
    13981 atccaagcaa caagcacaaa tgcagtgacg gctcaacaaa tcgctaatac aacgtcacaa
    14041 gtattccaag agaacgccaa agacgtgatg aatgtcgata aaatctcgat catctcaggt
    14101 gcagttgcca atacgacacc tgtttcgcca aataataaat tgaatctagt gattggctta
    14161 gtgttaggaa tgatggtagg cgtaggtcta gctttcttat ttgaattact cgaccggaca
    14221 gtaaaagatg aaaaatatgt gacggataca ttgggcttcc cgatcttagg gactgtacca
    14281 gaaatgtcag cgaaagaatt gaatgctggt atccgtaaaa aaggaccagc tccgacacaa
    14341 ccaaaagcta caaacgataa aacagcgcca tcacgcagaa gccgctcaag agtctaggag
    14401 gaaagaaaaa tggcacgaac acagaaacaa aaagcagccc aaactcatgc agtcagtttg
    14461 gtcacactca caaacccctc ttcaccaatc gctgaacaat atcgtacgat tcggacgaat
    14521 atccagtttg cctccgcagc tggtcagcaa attaaaacga ttgttgtgac ttcatcagga
    14581 cctggagaag ggaaatcgac cactgcagca aacattgccg ttgtttttgc caaatctggt
    14641 caacgggtgc tgctagtcga tgcagactta cgtaagcccg tcatctataa aacgttcaag
    14701 ttgaacaacg caagcggatt aagtaccgtt cttagttcat caggaagtgt agcagatgag
    14761 atccaacgaa caccagtcga gaatttgtcg attctgccaa gtggacctaa accaccaaat
    14821 ccatcagaac tactaagttc gccaagaatg gatcagattt tagcagaagc aagacaacta
    14881 tttgatgtag tgatcttcga catgccgcca gtagttgccg taacagatgc acagatcatg
    14941 tcttccaaaa cggatggcac cttattggtc gtacgtgaaa atatgtcacg gaaagaatca
    15001 ttgactaaag caaaagaatt gctggatatg gtacaagcgc gagtccttgg tgttgtatac
    15061 aatggggcag aacacagcaa agatgctggc tattactacg ggaattaaga aataaaaaga
    15121 gaggagacaa gagaaatgat cgatctacat tgccatatcc tgccaggaat cgatgacgga
    15181 gcagaaaaca tagatgccag tatcgccatg gctgaaaaag cgattcaaca agggatcaca
    15241 catattttat gcacacccca tcataataat ggaaaataca gcaatgaaaa atcacaagtc
    15301 atttctctag tcgcttcgtt acaagcagaa ttagagaaaa gacaattgcc tttgacgtta
    15361 ttggaaggac aagaagtacg tattaccgga acactgatcg aagacatcga ccgagatgaa
    15421 attttattta cagatttaga tgatacatat cttctaatcg aatttccgac actggaagtg
    15481 ccgttatatg cagaacagct cttttttgag ttgttacaac taggaaagac accagtgatc
    15541 gtccatccag aacgcaacgc gcattttcgt aaagacccga atcatatgct gccattccta
    15601 gatatgggat gcttggccca actcaccgca ccaagttatg tcggtgtatt tggaaaagac
    15661 atccagaaaa cagccaaaat gatggtcaaa cataatcttg tgcagatggc tgcttctgat
    15721 gcccatgggg tctctaagcg caccttttac ttaaaagagt gctataagca gattgccaag
    15781 gactttggta aggaaaaagt agtacagatg aaacaagtcg caaaggatct gatcaacggg
    15841 gatgaaatca gttatccagt ctatgaagag ataaaaaaga agaagtttgg tttattttag
    15901 ggggagtgta aggtgtttgt attaacaaga agaagaaaaa ttgccacatt ggttgttgtg
    15961 gacagtttgt tattgatcgc tgcaaatatt gcagcagcta aattcatgaa gccgtttgta
    16021 gctataccaa tggaccttat tttaatctct accggtttgt cgattggttt ttatttattt
    16081 tatggtagtt tattcaaagt tttcactcgg atcaatcgct atacaaattt aagagagatc
    16141 attgcaattt ttggttcact atctgcaagt gacgtctcaa gtatcctcat actgctgttc
    16201 attaatcggc aatacagttt acggttagtt atctttgctt atctgctttc gctcttattg
    16261 atcatcggta gccgattgat ctggcgtatc tacatcgaaa caaaaaatat gagatatgta
    16321 tcagcggatt ctgctaaaaa tacattgatc gttggtgctg gcgaaggcgg gcgtatttta
    16381 tacaacagct ttctgggatc caaaacggct caagatatcc aagttgtcgg atttgtagat
    16441 gatgatccga ataaaagaaa tacgtatctt tcaggaaaaa aagtattagg tgcattaaaa
    16501 gatattccgg aattgattga aaaatatgac attcaaatgg taacgatcgc tatcccttcc
    16561 ttatcaagaa aaaaattacg aagaatcttc gagttagtcg aatccgctca cgtgaaagtc
    16621 aacacgatgc cttctataga agaacttgct tcaggaaaaa ttagcgtatc aaaattaaaa
    16681 acaattgacg tagtggattt actgggaaga gatgaggtaa agctggatat tgaatccatc
    16741 aaagatcaga tcacagacaa agtcattctt gtcaccggcg caggaggatc gatcggttca
    16801 gaaatctgtc ggcaaatcat tcaattcaat cctgcaaagc tattgctttt aggacatggg
    16861 gagaactcga tctacttgat tgaccgtgag ttacgcacgc atcatcaaaa ttgtcctaca
    16921 gaaatcgtac cgatcatcgc agatatccaa gatcgcgaga aaatcaatga gatcatggaa
    16981 caatatcatc cagatatcgt ctaccatgca gctgctcata aacatgtacc actgatggaa
    17041 tataatccaa aagaagcagt caaaaacaat attttcggta caaaaaatgt agcagaagca
    17101 gctaaagcag caaaagtgaa aaacttcgtc atggtatcga cagataaagc taacaatcct
    17161 ccaaatgtca tgggttcaac aaaacgaatc gctgaaatga tcgtgactgg attgaatgaa
    17221 gaaggctgta cgaaattctc agctgtcaga tttgggaatg tccttggttc tcgcggtagt
    17281 gtgattccag tattcagaga acaaattgct aaaggtggtc caatcactgt aacagacttt
    17341 cgtatgaccc gatacttcat gacgattcct gaagctagtc gtttagttat tcaatctggc
    17401 gcattagcaa aaggtggcga aatttttatt ctagatatga gtgaaccagt gaagattgta
    17461 gacctagcca aaaacatgat ccgattgagc gggtatagcg aagacgaaat cgaaatcatt
    17521 gaaacaggta tccgtccagg ggaaaaacta tatgaagaac tactgttgga taaggaaaga
    17581 aatgatgaag cagtttatga gaaaatcttt gtaggaaaca tcaaaggtta ctcaatacaa
    17641 aaagtgatgg attttgtcaa gagcctgccg caagacgatg aacaattggc taaagatatc
    17701 gtcacttttg caaatgcttc gaataaatag gggggaaata taaatgaata aattagaaaa
    17761 acgtcagata ccattttcac cgccagatat tactgattta gaaataaaaa acgttgtaga
    17821 agtattaaaa tctggatgga taactacggg accaaagaca aaaaaattag aaaaaaaaat
    17881 tacaaagtgg ttaggaactt ctaaagtagt ttgtttaaat tcacaaactg ctgctgcaga
    17941 aacagggtta cgtttgctgg gaataggaga aaatgatgag gttatcgtcc cagcttatac
    18001 ttattctgcc agtgcatcgg ttgtatgcca cataggcgcg aacctagtat taatagactg
    18061 tcaacaagat tctttggaga tggattacga tgcattagaa gcagcaatta cggagaagac
    18121 aaaagtgatt attcctgttg atcttggtgg tataccttgt gactacgacc gtatttttga
    18181 gattgtggag aaaaaaagaa atttatttca acctagtaat gagattcaga aagcttatgg
    18241 tcgtataatt gttatggcag attctgctca tgcatttgga gctaattggc atgatcagat
    18301 ggttggtaat gttgctgact tgacaacatt ttcatttcat gctgttaaga attttacaac
    18361 tgcagaaggt ggagctttag tatggaaaga gcacgacaaa ctagataatg aggcaatgta
    18421 tcatcagtgc cagctgcttt ctttgcatgg acagtcaaaa gatgcactta ctaaaacaca
    18481 gttgggtgca tgggaatatg atattgtggg tccatggtat aaatgtaata tgactgatgt
    18541 tactgcatca attgggttgg cacaatttga acgctatcct aatattctga aaagaagaaa
    18601 gaatataatt aagaggtatg atgaagcctt tcaacaacta ggaattgaag ttcttatgca
    18661 ttatacggat gattatagtt cttcaggcca tttatatatt actagagtac ctaacattag
    18721 ttctgagcaa agaaatgaaa taattataaa aatggcagaa caagggattg cttgcaacgt
    18781 tcactataaa ccacttccaa tgtttacagc atataaaaat atggggtttg atattaataa
    18841 ctatcctaat gcctatgctc attttgtaaa tgagattaca ttaccattgc acacgaaact
    18901 tacagatgaa gaagtagaat acgtaattaa taactatagt aagattgtaa aggatttggt
    18961 tggatgatag gagaggcaaa atgagaaagt gggaagattt gccaaaagaa atgcagaatg
    19021 atgaaatacg gtattattat gatatcctca gtaaaaaaaa attcagctta gtggtaaaaa
    19081 gagtatttga tattttagtg tctgcaactt tgttactgac actttcatgg ttatttttta
    19141 ttcttgcaat agtgataaaa attgattctc ctggacctgt attttataga caagaacgta
    19201 ttactcgtta tggaaaaaaa tttaaaattt ataagtttcg tactatggta ataaatgcag
    19261 ataaagatgg cactcttgtt actgtgaaaa atgatgatcg tattacaaga gtaggaaagg
    19321 taatccgtaa atatagatta gatgaaatta gtcagctaat taacgttttg gtcggagata
    19381 tgtcttttgt aggaaccaga ccagaagttg gaaaatatgt aaagtcgtat tcaccattaa
    19441 tgttagccac attattatta ccagccggga ttacatcaga aactagtatt ttatacaaag
    19501 atgaagataa attgttgcat gataaaaata acatcgacaa aacttatata gaaaaaattc
    19561 ttcccagtaa aatgtattac aatttgaaaa gtatcagtga atttagttgt atgaatgatt
    19621 tgaaaataat gtgtaaaact atttttgtta tatttggtaa agaatatcac gccgacttag
    19681 aaggtgaacg agagtttatt aatcagataa aatgaatgga ggataagagt tttggtgatt
    19741 gaagagataa aaagttcgga taaagagtta attaattcga ttgtcgatat tcatatgaga
    19801 acatttacaa attttttttt gactttcatg ggaaaaaagt ttttggaatt gatgtatcgt
    19861 tcttacacag aatataatga ttctggtata ttggttgcta ttcaaaatga taagccaata
    19921 ggatttcttg catattctgg agatatgagt ggtttatata agtatatgat aaagaaaaga
    19981 ataataccgt ttggatttta tagttgtgta gcctttataa agaaacccaa tatccttatg
    20041 aggcttttta gagcttttct aaaaccaagc gaaacaaaac gtgatgaaaa ttatgtggaa
    20101 ttagcatcca ttggagttaa tccgaaagag aaatcgatag gagtaggttc catgcttata
    20161 gataacttaa agagtagaat tgatttttca aaatatgaat atattgcact agaaacagat
    20221 gcattaaata atgatgttgt gaataatttt tataaaaaga atgagttcat tttagaacgt
    20281 aaatattcta ctaatgaagg aagaattatg aatgaatatc ggttttatat ggagaagaat
    20341 taattatgaa attattgtat attttaaatg tagctaataa agtaaataac ttttctaaag
    20401 ctagtatgat ggcagctaaa caaacgggtt tagaatttta tatagctggt aactggaaat
    20461 acaaatccga tgaggaaaga aaagctgatg aagtgaaata tggaataaaa atataccaag
    20521 ttgattttta tagaaaccca ctaaatttaa aaaatataaa agcttatcat cagattgtag
    20581 agattataaa aaatgaaaaa atagatatta tccattgtaa tacacctatt ggaggtgttg
    20641 tcggacggat tgctggtctt aaatgtaaag tcaaaaaagt aatttaccag gcacatggat
    20701 ttcattttta ttcaggtgct cctaaaataa attggtttat atattatcct atggaacgtt
    20761 ggctagctca ttatacagat gttttgatta caataaatca tgaggattat actttagctc
    20821 aaaaacttcg tcttaggaaa ggtggaaaag cttattatat tccaggtgtg ggtatagata
    20881 caaataggtt tatgtcttcc acatcaataa ggaattttag agaaagtata tgtaaagaac
    20941 ttaatatacc tgatacagca aaaatcattt tatctgtagg agagctaaat gagaataaaa
    21001 accatttatc agtaatacat gcattacaaa aaattccgct gcatacagat atctattata
    21061 ttatcgctgg ggtaggagaa atgagggaga cactacaaaa tattgctaat gaattaggat
    21121 tacatcagcg agttttttta ctaggatata gaagtgatat acccaaatta ttagctgctg
    21181 caaacatata tattctaccc tcattaaggg aaggcctcaa cgcctcttta atggaagcga
    21241 tggctagtgg tttaccatgt attgtcggta atataagagg aaatgtagat cttatagaag
    21301 atggtaaggg tggatttcta atcaatccta aagacgaaga agaaattgct ggtgcgataa
    21361 atagattggc tgataacaaa ttattgtgtg aatctatggg ggcacataat ttagacaaga
    21421 taaaagattt tgatttatca gttgtgataa accaaataaa taatgtttac catgaaattg
    21481 taagtaaata attgaattga aagtgatatt aatatgaaaa aaataaaaat tatgcatttg
    21541 ttgcaaagta atagattttc tggcgctgaa aatattgttt gtcaaataat gagtttattt
    21601 caaaataacg aaaaatttga gatgatttac gttagcccag atggtcctat agcaaatatt
    21661 ttaaa
//
LOCUS       DADXTH010000054        20744 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.054, whole genome shotgun sequence.
ACCESSION   DADXTH010000054 DADXTH010000000
VERSION     DADXTH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.054"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            362..1051
                     /locus_tag="IWI97_001870"
     CDS             362..1051
                     /locus_tag="IWI97_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2463366.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1038..2246
                     /locus_tag="IWI97_001871"
     CDS             1038..2246
                     /locus_tag="IWI97_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2463367.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2540..3811
                     /gene="serS"
                     /locus_tag="IWI97_001872"
     CDS             2540..3811
                     /gene="serS"
                     /locus_tag="IWI97_001872"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBC2463368.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4360..5844)
                     /gene="guaB"
                     /locus_tag="IWI97_001873"
     CDS             complement(4360..5844)
                     /gene="guaB"
                     /locus_tag="IWI97_001873"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBC2463369.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6349..7044)
                     /locus_tag="IWI97_001874"
     CDS             complement(6349..7044)
                     /locus_tag="IWI97_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBC2463370.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7134..8234)
                     /gene="ychF"
                     /locus_tag="IWI97_001875"
     CDS             complement(7134..8234)
                     /gene="ychF"
                     /locus_tag="IWI97_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBC2463371.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8243..8434)
                     /locus_tag="IWI97_001876"
     CDS             complement(8243..8434)
                     /locus_tag="IWI97_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBC2463372.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8455..9345)
                     /locus_tag="IWI97_001877"
     CDS             complement(8455..9345)
                     /locus_tag="IWI97_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBC2463373.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9332..10099)
                     /locus_tag="IWI97_001878"
     CDS             complement(9332..10099)
                     /locus_tag="IWI97_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBC2463374.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10111..10824)
                     /gene="rsmG"
                     /locus_tag="IWI97_001879"
     CDS             complement(10111..10824)
                     /gene="rsmG"
                     /locus_tag="IWI97_001879"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBC2463375.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11056..12957)
                     /gene="mnmG"
                     /locus_tag="IWI97_001880"
     CDS             complement(11056..12957)
                     /gene="mnmG"
                     /locus_tag="IWI97_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBC2463376.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12974..14371)
                     /gene="mnmE"
                     /locus_tag="IWI97_001881"
     CDS             complement(12974..14371)
                     /gene="mnmE"
                     /locus_tag="IWI97_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBC2463377.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14670..15053
                     /locus_tag="IWI97_001882"
     CDS             14670..15053
                     /locus_tag="IWI97_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463378.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15221..15979)
                     /locus_tag="IWI97_001883"
     CDS             complement(15221..15979)
                     /locus_tag="IWI97_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBC2463379.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15997..16815)
                     /locus_tag="IWI97_001884"
     CDS             complement(15997..16815)
                     /locus_tag="IWI97_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBC2463380.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16812..17168)
                     /gene="rnpA"
                     /locus_tag="IWI97_001885"
     CDS             complement(16812..17168)
                     /gene="rnpA"
                     /locus_tag="IWI97_001885"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBC2463381.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17286..17420)
                     /gene="rpmH"
                     /locus_tag="IWI97_001886"
     CDS             complement(17286..17420)
                     /gene="rpmH"
                     /locus_tag="IWI97_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBC2463382.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17943..19277
                     /gene="dnaA"
                     /locus_tag="IWI97_001887"
     CDS             17943..19277
                     /gene="dnaA"
                     /locus_tag="IWI97_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBC2463383.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19475..20605
                     /gene="dnaN"
                     /locus_tag="IWI97_001888"
     CDS             19475..20605
                     /gene="dnaN"
                     /locus_tag="IWI97_001888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBC2463384.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5941 a         4226 c         3803 g         6774 t
ORIGIN      
        1 tatcatgtca tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg
       61 ttcttttgaa caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt
      121 atttatcaac atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc
      181 tttataactc cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc
      241 ttattcacac caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt
      301 atctatttct gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt
      361 tatgaaaatt ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt
      421 tcacaatgag gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat
      481 cagaacaatc cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg
      541 gatgcaagta gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc
      601 aaaaactact gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac
      661 caagccattt aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa
      721 aatgcagatt gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa
      781 agactctcat gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt
      841 cggtatcctc tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt
      901 tgagcgtgtt tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag
      961 tcatttaaga gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg
     1021 gggagtgggc tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga
     1081 tcaatttgac ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc
     1141 ttttgcttct attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac
     1201 cttctttgac gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg
     1261 atttgttctg gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg
     1321 ttttgtattg gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg
     1381 aacttcacta catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg
     1441 acttaagccg agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg
     1501 aagacgaacg taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata
     1561 tccgtacccc actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc
     1621 actcagaaga agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga
     1681 aatctttagt ggatgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga
     1741 atttttctgc ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg
     1801 aagcttctaa aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg
     1861 atggcgatac cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat
     1921 acggaaaagg agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg
     1981 caacagtcaa aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc
     2041 gtttttatcg tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag
     2101 caatcgctca aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc
     2161 aatggacttc tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata
     2221 cacaggaagt tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata
     2281 aatgaaaaac acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta
     2341 aatcagcaaa ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa
     2401 ggtcggttca acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta
     2461 ccgcgaattt cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt
     2521 tcaaaaagga ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt
     2581 acaagaaaaa ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact
     2641 agatgaaaat cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga
     2701 tgtttctgct gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc
     2761 tgaaatgaaa gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga
     2821 tgaaaaattg aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt
     2881 tggaacagat gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc
     2941 ttttgaacca aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg
     3001 tggcgcaaaa gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga
     3061 acgtgctgtc tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat
     3121 gatcactcct tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt
     3181 caaagaagat gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt
     3241 accattaact aactattata atgatgaaat tttagatggt gcagatttac ctatttattt
     3301 cactgcacta agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg
     3361 actgattcgt cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca
     3421 ttcttacgaa gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca
     3481 tttgccttat cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac
     3541 ttatgactta gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc
     3601 taactgtgaa gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa
     3661 aatccactat actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc
     3721 cattttggaa aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc
     3781 ttatatgggg aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg
     3841 agtattaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat
     3901 accgagaaac tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc
     3961 tgagtattag agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa
     4021 taccgagggt cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc
     4081 tcctagttac aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg
     4141 tgattcggag gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt
     4201 aactccaaga aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt
     4261 cttatttccg aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca
     4321 aaagtgttct ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt
     4381 tggtgcttct tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct
     4441 catttggata aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc
     4501 cattcctgaa cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata
     4561 agcaacccga ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg
     4621 gaagtaacgg tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt
     4681 tttgaatcga cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa
     4741 catacttcct agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga
     4801 atatttgatc cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc
     4861 atcataaata gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat
     4921 cgatcctggt ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt
     4981 cgcttcagcc gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat
     5041 ttcttgaatt tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc
     5101 atctgcccct gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac
     5161 ggctgctgct acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac
     5221 tttctcgatg tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg
     5281 taatttttcg attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac
     5341 tggggcagtc actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt
     5401 tacaaaacgc atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc
     5461 aacgattggc acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag
     5521 atgctgtggc gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt
     5581 tcttacttca tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc
     5641 ttgtcttgcc atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat
     5701 gattggaata ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg
     5761 taacacgtga ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt
     5821 gaactttgtt tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta
     5881 aaataattga ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat
     5941 agaataccaa tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt
     6001 cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt
     6061 gggtttatta ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag
     6121 ctgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata
     6181 aattggactt aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt
     6241 gacaagactt ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt
     6301 ctttactacc cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg
     6361 gacggataaa cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc
     6421 agttgccccg ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa
     6481 tagtgttgtc gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa
     6541 tgtcttaccc attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat
     6601 gtatttgtac atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga
     6661 aaagccataa gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact
     6721 taatagacca agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt
     6781 ttttacttct gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa
     6841 ttgacgggct gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag
     6901 ggcttttact ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa
     6961 aatgtactgt tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat
     7021 tgctcgtaat tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa
     7081 cggtcgtcac aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca
     7141 ttaaagcgga ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga
     7201 acacgtccag cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag
     7261 acagtttctg cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt
     7321 ggtgctttga ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag
     7381 taagtagcta agcctaacaa atcataagca gcacggatca actgatccaa acctgattct
     7441 tcgattccta aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct
     7501 tcttctgcac gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc
     7561 acttcttgta cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct
     7621 acataaagaa ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct
     7681 tcagtaaatt cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc
     7741 agtacagcta gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga
     7801 gtatagcgtt tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca
     7861 atatcttcta gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag
     7921 cgaacaacgt gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa
     7981 ccttctcctt tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc
     8041 ggaacagttt ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact
     8101 tctaccatcc ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct
     8161 gcttttgtga tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca
     8221 gcagtcaatg ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta
     8281 atttcttttc aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga
     8341 ttttgatatc tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt
     8401 ttttcatttc aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc
     8461 ttcatcaaat tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata
     8521 ttcaatctcg atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt
     8581 atccatcaaa cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg
     8641 gatttttttc ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg
     8701 gcggacagtt aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt
     8761 ttttaaacct aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc
     8821 cttgacaaga tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt
     8881 tcctaaacga ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata
     8941 tgcttctgct tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac
     9001 ttgcatcatc gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc
     9061 tagtttggac gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga
     9121 acggcgtacg atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg
     9181 caaagatact tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa
     9241 tggtagctgg acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg
     9301 aaacagtgca tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct
     9361 ttgccaatgc ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt
     9421 ttccatgact aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt
     9481 tttcgcggaa gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat
     9541 catacattgt cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta
     9601 aacgaaccgt attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag
     9661 gtatcaaaat cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg
     9721 ggcaatcgat caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc
     9781 ttgattctct tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg
     9841 caggcacaat actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt
     9901 cattgactag aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac
     9961 tggtagcatt tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa
    10021 gacaagcacc tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa
    10081 cagatattat ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc
    10141 gggctttctt gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg
    10201 ttgatcagag acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa
    10261 agcaatcgct tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata
    10321 aaaatagcct tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc
    10381 aaccgcacga gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt
    10441 ttctgcccgg tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa
    10501 gaaggttatt cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat
    10561 ttttaaaggt atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg
    10621 cgtaaagtct acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc
    10681 tttttcatcc gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata
    10741 ataaatagcg aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga
    10801 ttggcgaaat tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca
    10861 cagattctct ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc
    10921 ttaaaaattt gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa
    10981 atatacacag aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct
    11041 atttattatt acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata
    11101 gaccattagg atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat
    11161 tgtttctggt tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc
    11221 tgcgtagtcg atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt
    11281 ttccatggct tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc
    11341 ttttcgtgtc aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc
    11401 aggacgtctt aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt
    11461 tgctaaaaag tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat
    11521 ttcttgttca actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa
    11581 gcctacacgg tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag
    11641 acggtattca gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa
    11701 atcatcgatc atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc
    11761 ctgaactttc aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata
    11821 accgcttgta ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa
    11881 tgtcggacgg agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat
    11941 catttctact ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg
    12001 taaagaagta gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa
    12061 aaataattga tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca
    12121 atagcgagcg cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc
    12181 tcgaataatt tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag
    12241 tttataggca ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg
    12301 ctcttctgta acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt
    12361 gaaccgttcg atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg
    12421 agagttattc ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc
    12481 tgttccggct gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac
    12541 tccatgacaa acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa
    12601 atttggttct ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg
    12661 tgctcgcaat gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata
    12721 ggttttatcg atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac
    12781 gcctttagct ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt
    12841 tattgtcagt aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc
    12901 ggcgtgtcct gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg
    12961 tttgttcctc cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat
    13021 cttgtacact gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt
    13081 gaacgagatc cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg
    13141 aagctttttc taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc
    13201 ctgtttctcc gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt
    13261 ttttagctac ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt
    13321 gttccagctg tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg
    13381 tcgcttccaa taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta
    13441 agatcaagtc tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca
    13501 catcttctgt ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat
    13561 tgacatactc ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt
    13621 cttctcttaa caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg
    13681 ccgtacttaa cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga
    13741 ttctttgttg gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat
    13801 catcatattc tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt
    13861 gtctcaatga gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca
    13921 ttgctttgtc tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa
    13981 cacgaccatt caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt
    14041 ctcttaacaa tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt
    14101 ctactacatc ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat
    14161 cgacaagctg attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta
    14221 cttctgataa tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat
    14281 cgccacttaa acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa
    14341 tcgtatcaaa ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac
    14401 aaaaaaagtg cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg
    14461 cttgttcttg attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt
    14521 taaaacaatt agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct
    14581 tggatgtctc tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt
    14641 tatctttctt aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg
    14701 aaaggagaaa atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc
    14761 ggatcaacga tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata
    14821 tcgtttttgt tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg
    14881 accttttctt aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg
    14941 cagtaatcaa ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg
    15001 aactttttgc ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac
    15061 agacaaaaaa agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt
    15121 agtgactact tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg
    15181 aaaaactttg tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag
    15241 gttctactac taaataacga taaggttcct caccttccga atgtgttttt acatgttcat
    15301 ctttgcttaa tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa
    15361 caggctgccc tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct
    15421 cttggcgctt ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta
    15481 aacgatggat gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc
    15541 caatcagtat gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc
    15601 tttccatttt gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg
    15661 ccaattccgt taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa
    15721 gaatcgtttc gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta
    15781 cagtctgttc tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg
    15841 cattctttcg acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga
    15901 cttggtcttt ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg
    15961 cagcttcata aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt
    16021 cattgctttc ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc
    16081 ttttcgaatt ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga
    16141 aactacccag tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac
    16201 aggcatcacg aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga
    16261 taaatacgta cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc
    16321 cgcgttccct aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat
    16381 agattgccat aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata
    16441 tggattgacg ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt
    16501 ttccggatct cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg
    16561 cgttttacgc atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat
    16621 cagtgtaaat aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc
    16681 agcaaagtaa taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc
    16741 agttccgcaa ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg
    16801 tttgtatttc ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg
    16861 tggactaaat tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc
    16921 acaataaaat ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt
    16981 tttcgtttga cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca
    17041 actcgaaaat gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca
    17101 aaagaatctc ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct
    17161 tttctcatta gttttctttc cttttctatc tctcacttac gctcataatc ataccataat
    17221 tacgattgtc ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca
    17281 gtggcctaag ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt
    17341 ccatttttag tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt
    17401 ggttggtatg ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca
    17461 cagacatact ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat
    17521 cgattgttaa aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag
    17581 ttatgcacag ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg
    17641 taaaaaaagt aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta
    17701 tatctgtatt ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca
    17761 cctgtgcatt agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt
    17821 ttgtcgattg tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac
    17881 tattgaaaaa aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt
    17941 atatggtatc cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat
    18001 cgcctgctag ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc
    18061 agctcgttgt tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga
    18121 caaaaatcgt tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta
    18181 ctggcgaaga agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac
    18241 ctagtccgct aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg
    18301 gtaaaggaaa ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta
    18361 tttataaccc gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg
    18421 caattggtca tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg
    18481 aaacttttgc taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc
    18541 aagaatatcg aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag
    18601 aagctacgca agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa
    18661 tcgtactaac cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt
    18721 ctcgctttgc atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag
    18781 cgattttacg taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct
    18841 acatcgcagg acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc
    18901 aagcttttgc tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga
    18961 aaacgttaaa atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag
    19021 tggcaaagta ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta
    19081 ttgttgttcc tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac
    19141 cgaaaatcgg agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa
    19201 aaatccagca gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa
    19261 actttttact aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa
    19321 aaaaacgttc aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg
    19381 ttttttgagt tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa
    19441 taataaataa acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag
    19501 ctttatgcag gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat
    19561 tttgacaggt gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc
    19621 tgatatatca attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga
    19681 atctactggt tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc
    19741 tgaagaaaca tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa
    19801 agcgaatttt atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga
    19861 aagccataac cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt
    19921 ttttgctgtc tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc
    19981 tgataattct ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc
    20041 agtagaacaa gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt
    20101 atctcgctca ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt
    20161 gttgttcaaa acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga
    20221 taccaatcgt ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt
    20281 tttagcggcg atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg
    20341 tttatctatt cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt
    20401 agaagaaagc ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc
    20461 ggattatatg aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat
    20521 ctctgctatt cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct
    20581 gattactcct gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa
    20641 aagaaataga aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct
    20701 gtttattttt gtttaaatca ccttttttag attaaatcgt ctta
//
LOCUS       DADXTH010000055        20097 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.055, whole genome shotgun sequence.
ACCESSION   DADXTH010000055 DADXTH010000000
VERSION     DADXTH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.055"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            69..503
                     /locus_tag="IWI97_001889"
     CDS             69..503
                     /locus_tag="IWI97_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2463385.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(581..1114)
                     /locus_tag="IWI97_001890"
     CDS             complement(581..1114)
                     /locus_tag="IWI97_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBC2463386.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1208..2386)
                     /gene="mutY"
                     /locus_tag="IWI97_001891"
     CDS             complement(1208..2386)
                     /gene="mutY"
                     /locus_tag="IWI97_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBC2463387.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2379..3176)
                     /gene="recX"
                     /locus_tag="IWI97_001892"
     CDS             complement(2379..3176)
                     /gene="recX"
                     /locus_tag="IWI97_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBC2463388.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3283..4665
                     /gene="rlmD"
                     /locus_tag="IWI97_001893"
     CDS             3283..4665
                     /gene="rlmD"
                     /locus_tag="IWI97_001893"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBC2463389.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4839..5417)
                     /locus_tag="IWI97_001894"
     CDS             complement(4839..5417)
                     /locus_tag="IWI97_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBC2463390.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5739..6209
                     /locus_tag="IWI97_001895"
     CDS             5739..6209
                     /locus_tag="IWI97_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBC2463391.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6317..7543)
                     /locus_tag="IWI97_001896"
     CDS             complement(6317..7543)
                     /locus_tag="IWI97_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBC2463392.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8297..8497)
                     /locus_tag="IWI97_001897"
     CDS             complement(8297..8497)
                     /locus_tag="IWI97_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2463393.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8631..9239)
                     /locus_tag="IWI97_001898"
     CDS             complement(8631..9239)
                     /locus_tag="IWI97_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBC2463394.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9324..9794)
                     /locus_tag="IWI97_001899"
     CDS             complement(9324..9794)
                     /locus_tag="IWI97_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBC2463395.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9842..10756)
                     /locus_tag="IWI97_001900"
     CDS             complement(9842..10756)
                     /locus_tag="IWI97_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBC2463396.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10902..11705
                     /locus_tag="IWI97_001901"
     CDS             10902..11705
                     /locus_tag="IWI97_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBC2463397.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11883..12830
                     /locus_tag="IWI97_001902"
     CDS             11883..12830
                     /locus_tag="IWI97_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBC2463398.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12898..13191)
                     /locus_tag="IWI97_001903"
     CDS             complement(12898..13191)
                     /locus_tag="IWI97_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBC2463399.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13219..13884)
                     /locus_tag="IWI97_001904"
     CDS             complement(13219..13884)
                     /locus_tag="IWI97_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBC2463400.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14024..14656)
                     /locus_tag="IWI97_001905"
     CDS             complement(14024..14656)
                     /locus_tag="IWI97_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2463401.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14663..15730)
                     /locus_tag="IWI97_001906"
     CDS             complement(14663..15730)
                     /locus_tag="IWI97_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBC2463402.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15727..16458)
                     /locus_tag="IWI97_001907"
     CDS             complement(15727..16458)
                     /locus_tag="IWI97_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBC2463403.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16627..17106)
                     /gene="greA"
                     /locus_tag="IWI97_001908"
     CDS             complement(16627..17106)
                     /gene="greA"
                     /locus_tag="IWI97_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBC2463404.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17242..18417)
                     /gene="mltG"
                     /locus_tag="IWI97_001909"
     CDS             complement(17242..18417)
                     /gene="mltG"
                     /locus_tag="IWI97_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBC2463405.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18621..19790
                     /locus_tag="IWI97_001910"
     CDS             18621..19790
                     /locus_tag="IWI97_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBC2463406.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         6081 a         4019 c         3230 g         6767 t
ORIGIN      
        1 gaaaacttgc catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg
       61 gctagaccat gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg
      121 tacggatcag gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag
      181 acaaagatga agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag
      241 gaactgtacg tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc
      301 tttctgaata cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg
      361 ctaagaatca aggctataat acgattttat taggggcaca gtccacagct gaaacttttt
      421 acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc atgcctcata
      481 tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt
      541 tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct
      601 tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga
      661 tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat
      721 tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag
      781 gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa
      841 aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat
      901 tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc
      961 catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt
     1021 ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga
     1081 tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt
     1141 gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga
     1201 gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca
     1261 tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt
     1321 tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat
     1381 gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa
     1441 agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca
     1501 gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca
     1561 cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa
     1621 aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct
     1681 ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa
     1741 aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta
     1801 gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg
     1861 cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc
     1921 tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac
     1981 ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg
     2041 tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc
     2101 gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat
     2161 ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg
     2221 tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat
     2281 tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa
     2341 attcttttgt ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt
     2401 tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt
     2461 acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct
     2521 tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc
     2581 gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc
     2641 aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt
     2701 gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt
     2761 ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc
     2821 atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt
     2881 aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt
     2941 cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg
     3001 aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct
     3061 tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat
     3121 aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac
     3181 atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat
     3241 atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc
     3301 aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc
     3361 ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta
     3421 gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa
     3481 acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa
     3541 ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca
     3601 ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg
     3661 gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact
     3721 caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc tataacagac
     3781 tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat
     3841 aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg
     3901 gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa
     3961 ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca
     4021 atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac
     4081 ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct
     4141 cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa
     4201 gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc
     4261 ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca
     4321 atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc
     4381 gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc
     4441 atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac
     4501 cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt
     4561 aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact
     4621 gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg
     4681 aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt
     4741 tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag
     4801 ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc
     4861 acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat
     4921 ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct
     4981 ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc
     5041 caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc
     5101 caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt
     5161 tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta
     5221 tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag
     5281 tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc
     5341 gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac
     5401 gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta
     5461 ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa
     5521 agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat
     5581 caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat
     5641 aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc
     5701 ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc
     5761 gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg
     5821 gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga
     5881 tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac
     5941 ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca
     6001 tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa
     6061 tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc
     6121 atcgcctggc aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat
     6181 tgaattattc aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca
     6241 catagaaaaa gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta
     6301 accaaactat ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac
     6361 cataaccatt ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg
     6421 tatagtactg acttgtatct tctgtttgct gataataggt attcggatct tggtactgat
     6481 catatgtgtt ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac
     6541 cgtttgtcga atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt
     6601 tcaaattagg taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga
     6661 tcgaaagaag atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt
     6721 cgccttgcaa ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc gtcgctacat
     6781 caagcatgtc atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt
     6841 ttctataatt gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt
     6901 cccgctgacg acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat
     6961 aggctaaacc tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca
     7021 aagtaaagtc aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca
     7081 tgccatccaa attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg
     7141 aatccatggc catttcgaca ccaccaaatg cgtaggcatg atttaattta tctaccgttc
     7201 cataacctac gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt
     7261 gtttcggatt aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta
     7321 atccaccggt atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta
     7381 ctctctcaga acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt
     7441 ggctcgcttc tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacga
     7501 atacccctaa caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca
     7561 cattttctaa aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata
     7621 agaaaacttt aaaaaacatt tttgtgtaac gattattatt ttttaaaatt tctcaaaatt
     7681 cttaactatc ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat
     7741 cgttttatct ttttttatgt attttttcca gaaaaagcga acaacaaggt attttccaaa
     7801 taatcgtgca gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac
     7861 cgttcagatt atcattcgat ttcacgacaa tttcttccta tgactggtcc ttttgttaaa
     7921 cccatcttct attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta
     7981 gtgattgcat taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat
     8041 tttcttttaa agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa
     8101 tggtctttta ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa
     8161 aatgttcctt gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc
     8221 caaaaaatct aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc
     8281 tttattcaac aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt
     8341 caacaatcgt gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa
     8401 ttgctgagaa atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt
     8461 tttcattgtt gaaccattta accgttccgt gttccataaa tgtatatcct ccaaaaaagt
     8521 tcttgcaagt aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg
     8581 ctcttttatt ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct
     8641 gcttcgaaac gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa
     8701 tctggtcgga catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga
     8761 attggtgtct ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat
     8821 tttccatttt ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt
     8881 ttgaattctt ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt
     8941 tctcctgtag gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta
     9001 gcatgtccgc caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata
     9061 tcagataata attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgaa
     9121 tttaaattcg tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg
     9181 tcaatataag gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata
     9241 aaaaaattcc tcctcaatgt tttcttaata atgtctacat tacaaagagt aacaaagaga
     9301 aggaaattct tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca
     9361 aaaacctcag tttctttaaa aataaacaac ttgctgaaaa tatagttcgc aaggataaca
     9421 ataatttgag tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat
     9481 accattgccc ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat
     9541 tcaataagtg catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt
     9601 acaaaagcaa acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa
     9661 tcaaaatatt ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca
     9721 aaaaagagat agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt
     9781 cgatactgct tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat
     9841 ttcaataact ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct
     9901 ctgcttggat cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag
     9961 gatatatctt ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa
    10021 taattatctt ttcgctatac tgccaaatat ggacatgatg aacatccttt accccatcga
    10081 ttgcttcaat ttcttgttcc attttttcat agtcgatagc aggtgcactt tgtatcaaaa
    10141 tagcggcagc ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca
    10201 gtgtaataac tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa
    10261 tcacacttac cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca
    10321 aactttcatc tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa
    10381 taactgctac tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga
    10441 atgcttcaac ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac
    10501 tcaatatttc taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca
    10561 ttttttgtgc cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat
    10621 ctgaaacaag agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca
    10681 ccgcattcaa aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca
    10741 ctattccttc ctccatttta tttatattgg atagtcttta gacaagaata acattttttt
    10801 gatatttcac caatcattca gctgtttcct taaagcactt gtatttcact tgatacttcg
    10861 ctagaattgc aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa
    10921 ttatcagttc ctttatacga ttcaaagacc taaaggaggc gagaaatgtc ccattttgga
    10981 aagccattgt ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag
    11041 tttttcaagt cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg
    11101 attttacaat ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa
    11161 caaactccat tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta
    11221 ctgatttgat ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc
    11281 ttcctaatac aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca
    11341 ccaatgaatc tttaaaaaac ttaacaggta agcagctccg ttctttttta agtgaagcat
    11401 ctataccact ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga
    11461 atctgctgat cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac
    11521 taacaaaagc tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta
    11581 ctataattgc aacaatcttg atggtctttc ttccttcttt tgacactagc gccttcattg
    11641 cttttgcagg aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca
    11701 attaagaaat aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac
    11761 atttttttgt cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa
    11821 ggcgcttcaa agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa
    11881 ctatgtcaga aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa
    11941 tccatactat agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa
    12001 atgtagagat cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta
    12061 ccttgaaatg ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt
    12121 caaagaaaca tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc
    12181 atgtcttagg aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaggcacaga
    12241 gtgacatgaa atggttaggt tcatcggtcc attaccaatc tatccaaggg aaaaaaatac
    12301 taatcattgg aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca
    12361 tgaactgcta tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt
    12421 ctttaaatca actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc
    12481 taacggagga aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat
    12541 ctgtattcat caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt
    12601 taaataacca gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac
    12661 aagatcatcc tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga
    12721 cacctgattt tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg
    12781 ccacccatga gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa
    12841 caatattttc aaaagaaaaa agagaaggtg acaaaaattg tgtcaccctc tcttttttta
    12901 ttgtttttta aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc
    12961 atatccttta aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catcaatttc
    13021 cactttcttt acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg
    13081 aacctctgtt ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc
    13141 aatttctgag tcaaaccact tttgtgcttt tggcgttacc gttaatttca tcccaacaac
    13201 ctactttctc ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt
    13261 ctctccaata accagtagat tatcattttc acgaacatat tcatctgctg caggagaagc
    13321 gataaccgtt tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa
    13381 atttaattca gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa
    13441 agaatattta tctgacaact ctaggtaatc taaaatattc tttgacacta aattatgcgc
    13501 aatccttatt cccatgtctc gctcgggatg gacgacgcga tcagctccga tcttttcaag
    13561 tactctggca tgatactcat tttgggcttt tgctaaaata tttggtacgc ccatttcctt
    13621 taccatcaga gtaactaaga tactggcttg aatatcttct ccaatagcga caattacatg
    13681 atcgaaattt cttatgccta atgatcgaag tgtcatttca tcctgtgcat ttgccacaac
    13741 agcatgagtg gctatattca tatattcatt gacccgatct tcattactat caatagccag
    13801 tacttcttga ccagcttcga tcagtgttcg gcagatactt ccgccaaatc gtccaagacc
    13861 tacaattgca aaattttgtt tcaccgtttt cctcctttag tatcttcttt tctcagtata
    13921 acaaagcctt tacaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa
    13981 tagttatttt ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat
    14041 gtttgaatgc atagatagcg gcttgtgtac ggtcttccac ttctagctta gccaatatat
    14101 tggaaacatg tgtttttact gttttcagtg taataaacaa ttcgtccgct atttcctgat
    14161 tactttttcc ttcagaaatc agcattaaaa tttcattttc ccgattcgtt agttcttcat
    14221 gtaatacagg ctcatttcgc cgactcattt tttccatcat ttttgttgtc acttctggtt
    14281 ctaatactcg ctcgccacgt tgcgttgccc ttatagcatc agcaatttca tgtgcggtag
    14341 aggtcttcaa caaataccct gctgcacctg cttcaatggc aggataaact ttttcgtcat
    14401 caataaaact ggttacgata atgattttag cttctggcca atcttttaag attgcttttg
    14461 ttgattcgat accatccatt tcttccatga ctaagtccat taaaatgacg tccggtcgaa
    14521 gtgtcatcgc cttctcatag ccttgtcgtc cattttcagc ttctccaatt acttcaatgt
    14581 cttcttgaat agataaataa gaagagacgc ctaagcgcac catttcatgg tcatctacta
    14641 ataaaacttt tatcatcctt atctatgcct ccttcatcaa agggaccttg atttctacac
    14701 ttgtaccttg acctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt
    14761 ctttgatatt gttcaatcca taacttcccg ttttcgtttc attcatatta aaccctgttc
    14821 cgtcgtcaat aatacgtaac aaaagattat tgtctatttt gtgcaaatat acctctaatt
    14881 cattggcttt agcatgtctt aatgtatttg ataacaattc ttgaacgatt cggaacaagt
    14941 gatcctcaat ggaactagat agttttacat cttctacatc ccatttcagc gaaatctgga
    15001 ttttattttg caattccttc aataactgct cgataccttg ctttaaactt ttctcttcta
    15061 aattgactgg acgtagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga
    15121 tctctgctac cattttcaac tgcttgcgaa acatttctgg tgtctcgctt ttttctgctt
    15181 gctccgtcaa tgccgacatc atcatcattg ctgcaaacaa ttgttgcgaa actgaatcat
    15241 gcagctcacg ggctaatcga tgtctttcta attctaaaat ttcttctttg gtttgtccat
    15301 ctacatattg gggacggctc gttacaattt gtaattcact agatacttca atcatttttt
    15361 cttttatctt agtgatttcc ttatcaatat ctttgatgta taaatcgtcc gatgcacttg
    15421 ggatcggaag aagcaacaat ttggattcat aattgccaga agaaagtgca cgcagctttt
    15481 cttcgatttt cccgtatttt gtcttttgaa taagtgataa taatagaaag acaattaatc
    15541 ctacacctaa ggaaatggct aacaaatgga agataagcgg aacatataat aaacgtgcac
    15601 gcagtaattc tccccaccaa tgactttgat tgctggcata aaaataagtg aaaagtgaaa
    15661 atagaataat aaggaaagcc gcaatccccg aataaacagc tagcatcgct ctggatattt
    15721 ttcccatcat attcgtatca cctcgacatc tccaatcaag gtattagtaa taatcttcaa
    15781 tctacgaggg ttttcatcat aatcactgct gtagattttg atttgttcat ttttcaaaga
    15841 aaattgctct tcttcaaaca agacatttcc tactaatgtc gcatgctcta atcggatggc
    15901 tacgcccaaa ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt
    15961 atcatctttt ggcaaaagcg tattgcccaa atctatgata gtatcaccag aaatgacggc
    16021 gatgttgata tcgtcccatt catagacgtc gtttccaata cgttggttac cgaataattg
    16081 ctgtcttttc cgctcattat tatgtgtttt taattgctct gtttggatca tcatgatctg
    16141 tttctttcgc caaaaagcat ttttggttaa atctatccct gaaatctcta cacctttcag
    16201 accaataaat aaaacggcaa atacagccat catccataaa gcaggactgt tgactaagct
    16261 aaaaaaaatg accaaacttc ctaatatcaa ttgaaaattt tgaaatttag tacgaggatg
    16321 tttgcgcata gctagatata tattgaaaat accgaatatc actaaaagca aaagtccagt
    16381 attgtttacg atttgccaaa cagcaaataa cagcagtagt gcttcaacta ccacaaaaaa
    16441 acgccaagaa ctattcatcg ctctttctcc ctcctgacac attctcattt catcatacct
    16501 atttttttaa ctgttggcta taggacttta gatgtaagat cgtttgttga aatctaaaat
    16561 acacaaagag aaaaagatct agagtgtatc atcagtaaga tgaactctct agatctttca
    16621 gtcagactac gcttgttcta ctttgacgat tttaactaac atatcgccac ctggagtagc
    16681 aatagttact tcatcatttt tacgtttgcc aatcaatgct tgcgcaatag gagaatcatt
    16741 ggaaattttg ccagaaaacg gatctgcttc agcgcttccg acaatcgtgt actcttcttc
    16801 ttctccatct ggtagttcga tgaatgtaac tgttttacca attgatacct catctgcatc
    16861 tacaccgtcg ttatcgatga tttgagcaaa acgaatcata ttttcaattg ttgtgattct
    16921 tccttcaaca aatgcttgtt catctttcgc tgattcatat tcagagtttt cggaaagatc
    16981 tccgaaaccg cgggcaattt tgattcgttc aatgatttct ccgcgtttga ctgtttttag
    17041 ttcttctaat tctttttcta atttttcttt accttctaga gtcattggat aaactttctc
    17101 taccatgtgt aacgtctcct tttttcgata aaataccgca aaaggtaatc ttttgaggaa
    17161 tatagtccaa aaaagattac cacgcttttc ttcaaatgta aatgtttttc taaaaatgat
    17221 caatgtctag agcctgtttt tctaagaatt atttacatat ttttccacta attccatatg
    17281 ttcttcataa gttttagcga aataaacatt tccagtgctc gtatctgcta cgaaataata
    17341 atagtcattt tctgctggtt ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa
    17401 aggtccaggt ccatagccgg tattggtata caaattatat ggtgaatcta cagctgtatc
    17461 ttcatacgtc actaattctt tgtgttcacc taatgcgtat aaaacagaga tatctgactg
    17521 aagtggcata tttgctttga ttcggttaaa gaagacttgt gcaatgtttt tacgatcatt
    17581 ttcttttacg ccttcttttt caactagaga agctagagtt aaaacttctt gtacagtcaa
    17641 atttttctgc ttgatagctg aataatagtt ttgcatgacc gtattcgtct tgttcaccat
    17701 ttcaatcact agatccttaa gcgaagtact cgtgtaataa tcatatgtgg ctgggaataa
    17761 gtaaccttcc aaacggtatt ttgtgttctc agctttagat gcactttcca acagttctgg
    17821 gaatttttgg tgcaactcgt taaagaaagt ctcatcattc actaaatcta aaaattcttt
    17881 cttgtcttta ccagttacct tcgctacccg ttcagcaatc tgatcgatat cgtacccttc
    17941 tggaatggca attttcccat ctgcaacttt tgttggctca gaagtaccgc cttcttgcaa
    18001 ctgtttgcca atctcgtcaa gagtcatatt aggcgctaac tgataatagc cagcttgaaa
    18061 gccagttaaa tttttaaatt ttgtataata attgaaaaca atcccgcttt taataatatt
    18121 gtccttctcc aaaatctcac caatctgctt gttagaagag ccactcggga tctcgacttg
    18181 gactaattgg tcatctgatt tatctaaagg cttcaacccg ctatctacat aacgatacac
    18241 ggtaaatcca agaaatccgc caataatcaa taatgtaaga gcaatcacta aaatgatctt
    18301 cctcacaatc ttatcttctt ttttacgcag atttctccgc ttgtcgatta tatctgctgt
    18361 agctgcctta ctggcttctt ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac
    18421 aactcctcct tactatcaag ttcgtaactt tattcattat acatgatatt tgaattggcg
    18481 tgaatgattt tatttaaata ttctatcaaa aatgtaagta gtaaatccat tactataaga
    18541 ttaaaataaa tgttatcaag atacaagcta ttttagcttt attctttctg taaaaaagaa
    18601 aggagactaa ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat
    18661 ttttaatgat cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga actattcttg
    18721 gatggcaatt ttacaataat gaacactctc agatagttgc ccatgctcaa tcagctcaaa
    18781 aaaagttgat actgactacc aatgaacaac agctcgttga aaaaatcgat caactattaa
    18841 gaaaaaatga ttatattggc tcaatttacg taaagaaaaa taatcgcatc atcatggaaa
    18901 aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa tgatccttct ctttattatc
    18961 agattggttc ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg
    19021 ggcacctttc tttagatact aagctggctc aattctatcc acaaattgct ggcagccagc
    19081 aaattactat ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact gcttctccaa
    19141 ccataccgat gactgatgaa gaagtcgttc aatttgcgat tcagcactta aaatttacag
    19201 attatcagac ctctcattat gattcattga actttaccat attgactgga attttgatca
    19261 aattaacaaa tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa cctttaggat
    19321 tacagcatac aaaattttat gaccaagtca aaaattcatc caatcatgca ctatcctatc
    19381 aaatgtctaa agaggatgat tactttaaac cactgacaga atctgaaaca gagatccata
    19441 acgaattagg gactggtaat atcagtatgt ccgtctatga tttaaatacg ctgtttacac
    19501 aagttttatc tggaaaacta gtttccaaag acttactctt ctcattatgg cgtgaaaatg
    19561 cgaatggaca tccatatagt ggtggggtat atagcggaga taattatatc ctgtcgcaag
    19621 gaaatatcga ctcttttgat acatctgcta tatttagcaa agattcacag gatgctatca
    19681 tcatggaaag caatataaaa gctgacaaaa ctatcaaact gcctactgct gatctaagaa
    19741 accaaatcta tgacctgatg gaaggaacta ctaagttggc aaatagctga tatgatgaaa
    19801 agatagcaaa tactacttct ttgaatagca tttgcttaaa aaaagtactc gactaaacgt
    19861 aggaaagagt gtgggacaaa tctaaaaatc aatttttgct acagacttta attccaaata
    19921 aacagaacag aaagaggtgt tcttctttct gttctgtcca aattattcta agatttctct
    19981 ttaccaacat tatttgacaa agggcctatt aaatctaaaa tagtatcttt agattgctaa
    20041 aatatgtgct agcaaatcat tcatggcttg ttcttatttt taattttatg caataaa
//
LOCUS       DADXTH010000056        20073 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.056, whole genome shotgun sequence.
ACCESSION   DADXTH010000056 DADXTH010000000
VERSION     DADXTH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.056"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            238..2469
                     /gene="pflB"
                     /locus_tag="IWI97_001911"
     CDS             238..2469
                     /gene="pflB"
                     /locus_tag="IWI97_001911"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBC2463407.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2713..3474
                     /gene="pflA"
                     /locus_tag="IWI97_001912"
     CDS             2713..3474
                     /gene="pflA"
                     /locus_tag="IWI97_001912"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBC2463408.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3559..4485
                     /locus_tag="IWI97_001913"
     CDS             3559..4485
                     /locus_tag="IWI97_001913"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBC2463409.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4667..5560
                     /locus_tag="IWI97_001914"
     CDS             4667..5560
                     /locus_tag="IWI97_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBC2463410.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5694..5819
                     /locus_tag="IWI97_001915"
     CDS             5694..5819
                     /locus_tag="IWI97_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2463411.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5953..6585)
                     /locus_tag="IWI97_001916"
     CDS             complement(5953..6585)
                     /locus_tag="IWI97_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463412.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(6733..7275)
                     /locus_tag="IWI97_001917"
     CDS             complement(6733..7275)
                     /locus_tag="IWI97_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBC2463413.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            7410..7799
                     /locus_tag="IWI97_001918"
     CDS             7410..7799
                     /locus_tag="IWI97_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463414.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            7803..8567
                     /locus_tag="IWI97_001919"
     CDS             7803..8567
                     /locus_tag="IWI97_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBC2463415.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            8696..9643
                     /locus_tag="IWI97_001920"
     CDS             8696..9643
                     /locus_tag="IWI97_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2463416.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            9773..10513
                     /locus_tag="IWI97_001921"
     CDS             9773..10513
                     /locus_tag="IWI97_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463417.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10804..11472)
                     /locus_tag="IWI97_001922"
     CDS             complement(10804..11472)
                     /locus_tag="IWI97_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2463418.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            11606..11893
                     /locus_tag="IWI97_001923"
     CDS             11606..11893
                     /locus_tag="IWI97_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463419.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            12309..13847
                     /locus_tag="IWI97_001924"
     CDS             12309..13847
                     /locus_tag="IWI97_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBC2463420.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            13844..14809
                     /locus_tag="IWI97_001925"
     CDS             13844..14809
                     /locus_tag="IWI97_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463421.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            14796..16118
                     /locus_tag="IWI97_001926"
     CDS             14796..16118
                     /locus_tag="IWI97_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2463422.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      16181..16285
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16359..17102
                     /locus_tag="IWI97_001927"
     CDS             16359..17102
                     /locus_tag="IWI97_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2463423.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            17117..18109
                     /locus_tag="IWI97_001928"
     CDS             17117..18109
                     /locus_tag="IWI97_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2463424.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            18121..18858
                     /locus_tag="IWI97_001929"
     CDS             18121..18858
                     /locus_tag="IWI97_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463425.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            18848..19570
                     /locus_tag="IWI97_001930"
     CDS             18848..19570
                     /locus_tag="IWI97_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBC2463426.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(19654..>20073)
                     /locus_tag="IWI97_001931"
     CDS             complement(19654..>20073)
                     /locus_tag="IWI97_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBC2463427.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         6838 a         3401 c         4095 g         5739 t
ORIGIN      
        1 atacaggtgg cgtctctttt tcattcattt ttaataaatc cttatacgtt tgcaaaaaaa
       61 gatgaaagtg ttcacaatgc ttaaatgttg gtatgtcaac gttgatttaa atatatgtaa
      121 ataaaacata aaaattgtta aataaaaaac aagaaaaccc ttgcaaagat agggtgctgt
      181 gttatactaa tcacgtaaaa actgttacat aaaacagaaa tttaggaggt ccatacaatg
      241 gagcaatgga atggatttaa aggtaccaaa tggaaagaag gcgtagacac tagagacttt
      301 attcaaaata actacactga atatcgcggt gatgacagct ttttggaagg cccaacagaa
      361 gcaacagaaa aattatggga tgaattgcaa aagttaaatg atatccaatt cgagcgtaac
      421 ggtgtctatg atatggacac agaaatcgta tcaactatta cttcacacga tgcaggctat
      481 ttggataaag atttggaaaa aatcgtcggt ctacagacag acaaaccttt gaaacaagca
      541 ttcatgccgt tcggcggaat caatatggct aacaatgcgc taaaaagcaa tggctataca
      601 ccaaacgatg aattgacaca tattttcact gactggcgca aaacacataa tcaaggtgta
      661 tttgacgctt atactgctga aatgcgtgct gctcgtaaaa acaaaatcat tacagggctt
      721 ccagacgctt acggtcgcgg acgtatcatc ggtgactatc gtcgtgtggc attgtatgga
      781 atcgactatt tgatggaaca aaagaaaaaa gatcatgata tgactggtta tcatacaatg
      841 agtgaagaag tgattcgttt acgtgaagaa atcactgaac aatatcgtgc attagcgcaa
      901 atgaaagaaa tggctgcgaa atatggctat gatatttcta aaccagctgc aaatgcaaaa
      961 gaagctgtcc aatggctata cttcggatat ttagcagcaa tcaaatcaca aaatggtgct
     1021 gcgatgtcaa tcggacgtat ctcagcattc cttgatatct atatccaacg tgatttggat
     1081 aatggagtaa tcacagaaaa agaagcacaa gaattgatcg accacttagt aatgaaatta
     1141 cgtatggtca aattcgcgcg tacacctgaa tataaccaat tattctcagg attccctatc
     1201 tgggctactt tatccattgc aggtatggga ttagatggtc gttcattagt tacaaagaat
     1261 gatttccgtc tgcttcatac attaacaaat atgggacctt caccagaacc aaacttgact
     1321 gttttgtatt ctgaacacct tccagaagga ttcagaacat atgctagcag aatcgctatc
     1381 gaaagttctt cgattcaatt cgaaaatgat aatcagcttc gtgaacaatg gggttctgat
     1441 gattgtgcga ttgcttgctg tgtatcagga acagttgtcg gaaaagacat gcaattcttt
     1501 ggcgctcgtg ctaacttggc taaaacacta ttatatgcca tcaatggcgg agtcgatgaa
     1561 gtgactggcg cccaagttgg accagaatat cgtccaatca catcagaaat actagacttt
     1621 gaagaagtga aacataactt catgaatatg atggattggt tagcagaact atatgtcaat
     1681 acgttgaaca tcattcatta tatgcatgat aaatattctt atgaagcagc tcagctggct
     1741 ttgatggatt ctgacttgca acgcacattt gctacaggta ttgctgggat ctctcatgcg
     1801 gcagacagct tgtcagcgat caaatatgca aaagtaaaag caatccgtaa tgaaaaagga
     1861 atagcagttg attttgaagt ggatggcgac ttccctaaat acggaaatga tgatgaccgt
     1921 gcagatgaat tagtttcatg gatcttggaa tacttcatgg gacaaatcaa acgtcataaa
     1981 acttaccgca actcaaaacc aacaacttca ttattgacga ttacttcaaa tgttgtttac
     2041 ggtaaaaata caggaaatac tcctgatggc cgtgaagctg gaaaaccact tgcgccagga
     2101 gcaaatccaa gttacaatgc ggaacaaagt gggttattag ctagcctgaa ctcaacagct
     2161 aaaatgaatt acggctgtgc attggatgga atttcaaata cccaaacaat caatccttca
     2221 ggtcttggaa aagaacaaga tacaagaatc gaaaacttgc gtaatgtctt agatggatac
     2281 ttctcaaaag gagcttatca tttgaatgtc aatgtattca gtaatgattt attgcgtgat
     2341 gcaatggaaa atccaatgaa atatccgaac ttgacgatcc gggtatctgg ttatgcagtt
     2401 aaattccgtg acttgacaag agaacaacaa ttagatgtct tgatgagaac ttcacacgac
     2461 agaatgtaaa aagttcgtga aaaaagtttt tcgacttagc gtcgaaagga cgttgaatag
     2521 ccatcgcatc aaaatcgaat aatcagtatg cttatttgaa agaaacgatt tacaggggca
     2581 ggacttgtat gttccgtccc tgtaaatttt acacataacg aagcggcaat tgcgaaaaat
     2641 tttgttcaga aagcttcgct tcgacagcta acttgtactt aaaatgaaat caaagaaaag
     2701 atgtgaactt aaatggaaga aaaaacaatc ggctatgttc attctatcga aactttcggt
     2761 tcagtagacg gacctggact gcgctttgtc gtgtttatgc aaggatgccg catgcgttgc
     2821 caattttgtc acaatccaga tacttggaat attggcggag ggaaagaata cacagcagat
     2881 gaacttttag ataaagcaga acgcttcaga ccttactggg gagataaagg tggtattact
     2941 gttagtggtg gagaacctct attgcagatc gattttttga tcgaactatt taaaaaagca
     3001 aaagagcgga agatgcatac tacattggat acatgcggaa aaccttttac gtatgaagaa
     3061 cctttctttt cacgttttca agaattgatg aagtatacag atttactgtt atttgacatc
     3121 aaacacatcg ataatgaaga gcataaaaaa ctaacgcatt gggacaatga aaatatttta
     3181 gaaatggctc aatatttgtc aaaaatcaac aaaccggttt ggattcgcca tgtactagtt
     3241 cctgagcgaa gtgattatga tgaatatttg atccgactag acaactttat tcaaaccttg
     3301 tcaaatgtag acagagtaga gattcttcct tatcatacga tgggaaaata caagtgggaa
     3361 acacttggtt tgaaatatcc attagaaggg atcgaaccac ctacaaaaga acgtgtcgaa
     3421 aatgctaaac gattgcttca tacagaagag tatacaaaat atttaacgcg ataggtcgca
     3481 tcggaaagaa gataagtatt gcttctttcc gtttttttgc tattattaaa taggagaaat
     3541 cacaaaggag cgaatgacat gtcaaaaatt ttagtttttg gtcaccaaaa tccagataca
     3601 gatgcaatcg gggcagccat tgcgttttca cacttgcaaa atgaattagg taaagaaaca
     3661 gaagcagttg ctctaggtac acctaatgaa gaaacacaat acgcattaga ccactttcag
     3721 ctatctgcac cacgagtagt agagtcagct aaagcagaaa atgcagatgt catgcttgtg
     3781 gatcataatg aattccaaca aagtatttca gatatcagcg aagtaaatat tttagctgta
     3841 gtagaccacc atcgtattgc aaactttgaa acagctaatc cattgtacta tcgtgcagaa
     3901 ccagttggat gtacaagtac gattatttta aaactatata aagaaaacca agtaacaatt
     3961 cctaaagaaa tcgcaggaat catgctctca gctatcattt cagatacatt gttgttcaaa
     4021 tcaccaacat gtacgcctga agatgaagca gctgcaaaag aacttgcagc aatcgctgaa
     4081 gtcgatctaa acacttatgg acttgaaatg ttgaaagcag gaacaaatct aagtgataaa
     4141 tcagctgcgg ttcttcttga tttggatgca aaaagtttcc caatgggcag ttccaacgtt
     4201 cgtatcgctc aagtcaatac agtggacctt aatgaagtga tggatcgaca agcagagcta
     4261 gaagcagcaa tggaagatga aatgaaagca aatggctacg atctgttcgt attagttgtc
     4321 acaaacatct tagacagtga ttcagaatta cttgttgctg gtaaaggaaa agaaaaagta
     4381 gaagaagctt tccaaacaac tttgaccaac aaccgtgctt tgcttaaagg tgtcgtttca
     4441 cgtaaaaaac aagtcgtacc tcaattgacg gctgtattta gctaatcaaa tggtagtatg
     4501 aagcattcaa aatgaatgga taagcgattg atataaaaag aaacatagaa catactaaag
     4561 gccgaagaaa tggaggagaa taacagtcaa ttatgactct tgttcttcct cctttttacg
     4621 taaaagcctt ttaagtaaaa taaaacggat agaaaagaga acatatatga actattattc
     4681 aaaaaataag gaaaactttt atcaaaaact gaccgatgat ccgctttttt cttcattgac
     4741 cgattatcta tatgaacatc gagaacaaga aacgattcta agagaattga aaaaagaatt
     4801 ttcgcaaaat aaattttccc attttcttga tttattgatc gatgcaggat taatcaagag
     4861 ggaagaacga agatatcatt tgaactttcc aatatttgat cctaaagatt acttgcaaca
     4921 agccacttcg gctgctgaaa ccatagctga acaattgaag agactctctg ttgatgaaca
     4981 aaaattagcg atgggagaag ttatttgggc gtattgtttc gaagatgaac ggaaagaagc
     5041 gtatttttat ggagtaagaa acagccgaga gacagaattg ctgaggacaa ctgcaggaaa
     5101 tgagaagtac cgttttatca cactttcctc tatagaacac ttcccgctta ctttggctaa
     5161 ttactttttt gtacagaaga atcagcttcc tgtaacgaag gcattcaaag aattagccga
     5221 acttattgga gacgtaaatg aatcgtactt ttttgatcaa atagaagtta tcgttgacag
     5281 gatacgtaag aataaatata aaaatcgccg gcctagtatt ttccatcagt ctctacttgt
     5341 gacgaatact ataaaggaag aagaatcatt cacattagaa ttacctattg tcgaaaaaaa
     5401 caattttgag attgaattac caacattgga tccttcattg acaatggagg aaatggcctt
     5461 tcttaaacga caaatctttt ctgagctgag caagaaattt ataccgcatg cattttctta
     5521 tatcaaagaa tatggaacag ttttagtgag taaaacctaa gagagcagta agcaaaaatt
     5581 atactgactc ttgcaggagg agcctttctc ttcctttatg gttaaaagaa ttatttgagt
     5641 attttgccta tctaccacct attagattca ctacatggtc tggaaacaaa ggaatgcaaa
     5701 aaaatggtga gaaatgtgga atgacaaagg aagtagtgat aagaaaagtt agatttttaa
     5761 ataatcaata ttatgattca gtcaagtatg gcattttatg ggaagaacta gctgattaga
     5821 ttaggaagtc catttgataa tccagaatca ctaagaaacg aatggcgagt cattaataat
     5881 ttatccaatc ttgggttatt cttttagatt gaagtgttta aaactaaaaa agcatttatg
     5941 aagtcgtaat agtcaggctt caaaatgctt tttattttgt ttgttttttc tttgccacat
     6001 aaatacacct agactgacga tgaagagcat atccaacaga tttcctgcga cttggatcgt
     6061 cgaagggttg gtcagtggca ttaaagcaag gacaaaaaag gaaaacaagc tgtaaataaa
     6121 acaggtgaca ggaactaatc gaaagaagag cggacatttc aattggctaa aaatgagtga
     6181 aaaaataaaa taagtaaaaa tggataaaat cacgactaaa ccattcatga taaagaagaa
     6241 aagcgtattg tgattaaacg aataaaaaag aattgctaaa aaagaaaacg ttttaaatgc
     6301 aatcaatacg cttgaagctt cgtttaacgt accaggatgt cgatgaaaaa aataggcacc
     6361 gaaataaaca gaaaacagta agccgcaaag agaagacaaa atcaaaaaaa acggattttc
     6421 ttcggcttct gtgatatcag aatgatccaa aagcaaccag cctaatgcac tagaaggcgc
     6481 atgtgaggtc ttaaggtatg aataacatag aaaaaataag atcgtcaaag ttgcaaaaaa
     6541 agagcctaaa tgtaaaaaat tagtggttct acgttgaatt tccataatat ctccaaatta
     6601 aattgataag tatataaagt ataataacaa attgatgagt atatgtcgat gttttttaat
     6661 caaaataata aataaaaact gtctaacatt taaaatttaa tttagatatt agacagtttt
     6721 taagtttgtg agttattttc cttgtcgttt tctcactttt ttagtccaga aaccacctga
     6781 aatatatttt ggctcataag aaatgataaa agcacgctct tcgatttctt tgatcaggcg
     6841 atacagttca cgttcgcttt ttctaggaga aagtatttcg agaactagcc gctcaccttc
     6901 cagccccatt gcagaagtct gtgtcacacc ataaccgtgt tctctcagta tacccggcaa
     6961 acttttatct tctgcgctat ttgctggcaa aatcgttgtg accatgatgt atcctaatgc
     7021 caatttgtct tcaatcaaaa ttccgacact gatacctact gcgtaaccta acgcatagac
     7081 aaataaattc agtggattat ctaaacgatc taaaacaagg gataagccga cgatataaat
     7141 cgtgatctcg accatactga caagtggagc aatcagtcga tagcctttca ttgtcaacat
     7201 gaaacgtaac gtgttcagag tgatataagc aaaattaatg aggaagatca tccccaacat
     7261 ttttggatca atcatttgta aaactccttt catttatata acaacaatat caaaaaaaat
     7321 cgtaagtgaa gaaaaattga aactttatac atacaaaaaa atgataaaat gattataata
     7381 gaattataaa gataggttag gtgattaaaa tgaaaacggt caatcgtgtg ttttgtgcca
     7441 gccacgtgct aagtctcctt ctcagtatat acttaggtgt ctattcgatt caacagccaa
     7501 ttgaggtaga gaacattgtt tttcagatgt cgctgacaga tggtttaatt ttaagcgttt
     7561 ttttcttttt gatactgttt attggtaata ttttcggtag cgttgccagt tttttgaatt
     7621 tttctattta tccactcctt tcgctagcat taggagtggt gggactttgt tctctgtgct
     7681 tatttcccga gccattcagt cctgcattta tattatttgg tacactgaac ctgttccaga
     7741 caactgttgg agcatggttg ctatggcgat caggaaattt aatgaaaata ggtgagtaac
     7801 aattgaatcc aattatattg aaacgttctg tccatcatat tccagtgtta gaagttgttc
     7861 tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta ttatcacgga tggcagacaa
     7921 gtaaggagtt agttttgacg caaggaagaa aaattgcgca gcagggcatt cgtgtgatcc
     7981 tgccagatgc gatgaatcat ggggagcgca aacagccggt ttcttctatt ccatcctata
     8041 cattttggga tagtatctac ggaaatctgt ttgaatttga cacacttatc aagcatttag
     8101 aaaaaagagg tcttctacat gatcagcttg cagttggcgg ggtttcgatg gggggaatga
     8161 caacatgcgc cttgttggct aaacatcctc atatagtagc gggcgtgtgt ttgatgggtt
     8221 cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg cgcatcggaa tataattatg
     8281 tcgtaccaaa agactatttt gatttgattt catgggttaa gagctatgat ttgtcattgc
     8341 agccggaaaa gttagctgga cgtcctctgt tcttttggca tggacaagct gacgaaaaaa
     8401 tcccttatat acaaacggaa cgatttgtag aaaaaaatcg gcataagtct tacggaaaag
     8461 aaattgtttt tcgttcctca aaaacacagc gacatatggt acaaatacca ctaatggaag
     8521 aagcttccac ttttttagca aaaaatttga taaaaaagta taaataattg aaacggaaaa
     8581 tcactcatga cgatagagga ttttccgttt tttattttgg gctataataa actctgttag
     8641 gaaaatttgg tataattgat agtaagaatg acaacatgtt agaaaggaag agagcatgcc
     8701 cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa atgacggtct ctcgagtcat
     8761 caatcatccg gaacaggtga ctgacgaatt gaaagagtta gttttctcag caatggctga
     8821 actggattac cgtccaaata ttgctgcaaa agcacttgtc acgaatcggt cgcagatcat
     8881 caaattattt atactggaag aaatcgacac aactgagccc tattacatga atttgctgat
     8941 gggaatcgct aaacggatcg gtaaagcgca ttattctctc caattagtta caaatgatgc
     9001 cttcgacgtc ggttcttgtg atggatacat catcacaggt gtcagaaaaa aagacttcga
     9061 ttggatcgac cgtttggaaa aaccagttgt cttgtttggg gaaaattctg aaggatatga
     9121 ttatgtagac agcgataatg aatatggtac agcaaaagca acgcaatatg ccttagaaag
     9181 agattatcaa acgattattt atatcggtat cgaagtggac gagccttttg aaatttcaag
     9241 ggaaactggt tatttatctg tgatgaagga agttgatctt cctagtaaaa tcttgcggtt
     9301 tccaaatcat tcgacagaag tagaaagata tatgacagaa aactgggcag attttacaga
     9361 taaaacttgt tttgtatgta gttcagatcg gctagcacta ggagcagtaa gagggattat
     9421 aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt ggatttgatg gggtattttt
     9481 agaccaaatc agttcaccta aattgacaac gatcaagcaa gatattatac gtatgggtga
     9541 agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa ggggcgagtc aaggttatcg
     9601 tcgttttttt cctgaactag ttatcagaga gacaacgaat taagtcttat tactatatat
     9661 atatttatga aattcatcgc tattttctta aaataatcag cgcattgcta ctgaaaacaa
     9721 tgaatctgtg ctattattga gaaaagttcc atttttgaga aggatggttt gaatgaaaga
     9781 aatcagacga aaacatttcc gttttcctgc gtacgatgat gagttaggcg taaaattaac
     9841 caacgacaag aagcgagtat tatttgacgg acaagaagat cttttgactg aacagaatca
     9901 gtccccgttt gaacagatga aagaatttca tttcacgaac acgaacaccg agaaaatccg
     9961 tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg cgtaaggaaa atttggaaaa
    10021 gcataaagga aaattaccgg attataccgg ccattaccaa tcagaaatca catcgacagg
    10081 acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga caaagtgaaa caaggacaga
    10141 gtccaagcat actacacgaa acgcatctgt tgacacgaag gtgcgggagc gttcttattt
    10201 tgttcctaaa tatgtacccg catcgattat cccagaccct agagaaagca aaatttctga
    10261 ggatcattta atcgaatcga tgaaaaaaac ggatgactct tatctgttgt tcgatacaga
    10321 accgacgccg tatcaagaaa aaagaaatgg tgagccgtct gtacgaacat tcaatcgaac
    10381 aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa tcgaaaaaaa gccatggtat
    10441 cttagaacgc tcattacaag gattgatcga tgatcaagga aactctttgg gagaaaatag
    10501 ctattttaaa taagtgatat acaaatttat attcggctac agttggaaag gaaacgctgc
    10561 ggcgtttcct tattttatac tctatccgaa agtttatatc gtatcctcaa atttttttga
    10621 gtttaagcga attttttctc ccaataagta agaaaataat ggcaaaataa aaaaacaaac
    10681 tattttcgca gatcgtttgc aaaaatagtt tattttttta taaagaagat tttactgaat
    10741 aatatcattt tagtttacat aatgatattt ctgtaaatta aataaagtag tgaaaaataa
    10801 gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt
    10861 tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata
    10921 agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa
    10981 ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa
    11041 atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa
    11101 gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact
    11161 atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa
    11221 tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa
    11281 taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa
    11341 aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt
    11401 cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt
    11461 taattttttc atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac
    11521 tgacttatac tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt
    11581 tatttatcaa ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa
    11641 atcacccaaa aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc
    11701 tgtgacaatg gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa
    11761 agcaccactt gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt
    11821 ttattatcag tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa
    11881 agtggtgata taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt
    11941 ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa
    12001 aggtgttctc ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag
    12061 ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg
    12121 tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt
    12181 ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg
    12241 caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg
    12301 aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag
    12361 ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta
    12421 ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca
    12481 cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag
    12541 ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc
    12601 aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat
    12661 ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca
    12721 ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat
    12781 tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg
    12841 ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga
    12901 ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt
    12961 gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga
    13021 ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg
    13081 aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt
    13141 tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag
    13201 aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat
    13261 atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg
    13321 cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta
    13381 aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat
    13441 ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa
    13501 gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata
    13561 ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag
    13621 atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta
    13681 aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc
    13741 aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac
    13801 gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga
    13861 gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc
    13921 aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac
    13981 tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa
    14041 atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca
    14101 aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt
    14161 aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt
    14221 atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg
    14281 gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt
    14341 tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga
    14401 agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg
    14461 tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt
    14521 attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa
    14581 agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa
    14641 tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt
    14701 gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga
    14761 aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca
    14821 tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag
    14881 tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta
    14941 aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag
    15001 gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta
    15061 cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt
    15121 caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg
    15181 cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg
    15241 aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt
    15301 ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca
    15361 aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag
    15421 aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc
    15481 aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag
    15541 cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc
    15601 aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca
    15661 atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag
    15721 tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta
    15781 tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag
    15841 ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt
    15901 tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa
    15961 attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga
    16021 cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc
    16081 cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct
    16141 tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc
    16201 gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg
    16261 tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg
    16321 ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag
    16381 cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac
    16441 ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag
    16501 tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta
    16561 gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt
    16621 tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta
    16681 ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt
    16741 ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct
    16801 ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg
    16861 gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag
    16921 tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa
    16981 acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat
    17041 tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt
    17101 aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc
    17161 cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt
    17221 gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga
    17281 aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc
    17341 gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga
    17401 tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat
    17461 caaacataat acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga
    17521 ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc
    17581 tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga
    17641 agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat
    17701 gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga
    17761 acggttagat tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc
    17821 agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga
    17881 tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt
    17941 agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt
    18001 gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa
    18061 gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat
    18121 atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac
    18181 atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt
    18241 aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa
    18301 gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat
    18361 atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat
    18421 ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat
    18481 gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga
    18541 acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct
    18601 atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact
    18661 actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg
    18721 ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca
    18781 cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt
    18841 gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct
    18901 tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga
    18961 aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa
    19021 agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg
    19081 tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt
    19141 tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg
    19201 atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg
    19261 gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg
    19321 tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca
    19381 cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc
    19441 agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga
    19501 gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt
    19561 aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt
    19621 ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa
    19681 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    19741 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat
    19801 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    19861 tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    19921 ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc
    19981 agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc
    20041 ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DADXTH010000057        19603 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.057, whole genome shotgun sequence.
ACCESSION   DADXTH010000057 DADXTH010000000
VERSION     DADXTH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.057"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IWI97_001932"
     CDS             complement(287..649)
                     /locus_tag="IWI97_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463428.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IWI97_001933"
     CDS             complement(723..1103)
                     /locus_tag="IWI97_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBC2463429.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="IWI97_001934"
     CDS             1167..2360
                     /locus_tag="IWI97_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBC2463430.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="IWI97_001935"
     CDS             complement(2437..3021)
                     /locus_tag="IWI97_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBC2463431.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IWI97_001936"
     CDS             3222..4220
                     /locus_tag="IWI97_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2463432.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IWI97_001937"
     CDS             4332..4841
                     /locus_tag="IWI97_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBC2463433.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IWI97_001938"
     CDS             complement(4871..5530)
                     /locus_tag="IWI97_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBC2463434.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IWI97_001939"
     CDS             complement(5532..6296)
                     /locus_tag="IWI97_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBC2463435.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IWI97_001940"
     CDS             complement(6304..6981)
                     /locus_tag="IWI97_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBC2463436.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IWI97_001941"
     CDS             complement(7018..8403)
                     /locus_tag="IWI97_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBC2463437.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8372..8626)
                     /locus_tag="IWI97_001942"
     CDS             complement(8372..8626)
                     /locus_tag="IWI97_001942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463438.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IWI97_001943"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWI97_001943"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IWI97_001944"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IWI97_001944"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBC2463439.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IWI97_001945"
     CDS             9342..10199
                     /locus_tag="IWI97_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463440.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IWI97_001946"
     CDS             complement(10280..10615)
                     /locus_tag="IWI97_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463441.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IWI97_001947"
     CDS             complement(10682..12052)
                     /locus_tag="IWI97_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBC2463442.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IWI97_001948"
     CDS             complement(12130..12666)
                     /locus_tag="IWI97_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2463443.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IWI97_001949"
     CDS             12975..14456
                     /locus_tag="IWI97_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463444.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="IWI97_001950"
     CDS             complement(14559..15266)
                     /locus_tag="IWI97_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBC2463445.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="IWI97_001951"
     CDS             complement(15345..15683)
                     /locus_tag="IWI97_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBC2463446.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="IWI97_001952"
     CDS             complement(15794..16756)
                     /locus_tag="IWI97_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBC2463447.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="IWI97_001953"
     CDS             complement(16771..18102)
                     /locus_tag="IWI97_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBC2463448.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="IWI97_001954"
     CDS             complement(18110..18790)
                     /locus_tag="IWI97_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBC2463449.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="IWI97_001955"
     CDS             complement(19009..19488)
                     /locus_tag="IWI97_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBC2463450.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         5848 a         4031 c         3247 g         6477 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt tatctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat ctt
//
LOCUS       DADXTH010000058        19524 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.058, whole genome shotgun sequence.
ACCESSION   DADXTH010000058 DADXTH010000000
VERSION     DADXTH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.058"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(159..323)
                     /locus_tag="IWI97_001956"
     CDS             complement(159..323)
                     /locus_tag="IWI97_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463451.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            618..1946
                     /locus_tag="IWI97_001957"
     CDS             618..1946
                     /locus_tag="IWI97_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBC2463452.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2148..2981
                     /locus_tag="IWI97_001958"
     CDS             2148..2981
                     /locus_tag="IWI97_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBC2463453.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2997..3734
                     /locus_tag="IWI97_001959"
     CDS             2997..3734
                     /locus_tag="IWI97_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBC2463454.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3889..4857)
                     /locus_tag="IWI97_001960"
     CDS             complement(3889..4857)
                     /locus_tag="IWI97_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBC2463455.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5089..5922
                     /locus_tag="IWI97_001961"
     CDS             5089..5922
                     /locus_tag="IWI97_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBC2463456.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5956..6795
                     /locus_tag="IWI97_001962"
     CDS             5956..6795
                     /locus_tag="IWI97_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2463457.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6821..7381
                     /locus_tag="IWI97_001963"
     CDS             6821..7381
                     /locus_tag="IWI97_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBC2463458.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7580..9301
                     /locus_tag="IWI97_001964"
     CDS             7580..9301
                     /locus_tag="IWI97_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBC2463459.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9466..10608
                     /locus_tag="IWI97_001965"
     CDS             9466..10608
                     /locus_tag="IWI97_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBC2463460.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10624..11835
                     /gene="thiI"
                     /locus_tag="IWI97_001966"
     CDS             10624..11835
                     /gene="thiI"
                     /locus_tag="IWI97_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBC2463461.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12408..13055
                     /locus_tag="IWI97_001967"
     CDS             12408..13055
                     /locus_tag="IWI97_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBC2463462.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13184..13400
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13448..16093
                     /locus_tag="IWI97_001968"
     CDS             13448..16093
                     /locus_tag="IWI97_001968"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBC2463463.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16403..17716
                     /locus_tag="IWI97_001969"
     CDS             16403..17716
                     /locus_tag="IWI97_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBC2463464.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17706..18359
                     /locus_tag="IWI97_001970"
     CDS             17706..18359
                     /locus_tag="IWI97_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBC2463465.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18414..19106
                     /gene="radC"
                     /locus_tag="IWI97_001971"
     CDS             18414..19106
                     /gene="radC"
                     /locus_tag="IWI97_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBC2463466.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19256..19456
                     /locus_tag="IWI97_001972"
     CDS             19256..19456
                     /locus_tag="IWI97_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2463467.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         6577 a         3292 c         4138 g         5517 t
ORIGIN      
        1 gttcgagtcc tactgccgga gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact
       61 tgggtttctt tttcttttat tattatgatg aatcaagctt tctgtttttg tcacgtatgt
      121 tgtaatcttt acgaaagagt agagttggct actcataatc aaccatactt cccatcatcg
      181 ctaaatacga aataaatcag cattcctaca ccaatcaaag cacaagctgc accaagaaaa
      241 gggatgacat tcccaaagtt atctgtattc ataagtatgc caccacccac aaacaaaaag
      301 ccgccagcaa tcaggttttt cattttattc atctcctttc ttacgttata gtatttcctt
      361 ctttcaatga gtattttgaa agctatctta ttgtatgatg ttcttctagg cctcttcttt
      421 attatacagg ccattttcgt tatctcatag aaatattcct ttttatctga aaactattgt
      481 ataccgtatg acatttttct tcatgaagtt gtaagtatat tctcattcaa atgaaaattt
      541 catcatagaa gagattgtat ttctaaattc ttaagagtaa aatacgaagt ataacttgga
      601 tttggaagga tgattggatg aaagctatta cgcctgatat gacaacaaaa tttaacaaac
      661 agtttcaagc aaatatcaaa cagcaggcat tgcagcgggc agttgtgaag aacggaatta
      721 ctgcttctgc tgaaaatgtt tcagcacatg tggagaatac accggtattt tctatagatt
      781 tggctacggg gaaagtggct aatcaaatgc aatcggggcg ttgctggatg tttgctgcac
      841 tgaacacatt ccgtcataag atgctgaatc aattccattt aaaagagttt gaattatcac
      901 aaaattacac ttttttctgg gataaatatg aaaaggcaaa ttatttctat gaaaacatca
      961 ttgcaacagg aaaagaggca atcgatagcc ggaaagtagc tttcttactc gctactcctc
     1021 aacaagatgg cggccaatgg gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc
     1081 ctaaatcggt gatgccggaa agtgcgaata gttcaaattc tcgagatttg aataactact
     1141 tgaacaaatt actgcgtaaa gatgcagtag tgttacggaa aatggttgct caaggagaaa
     1201 cattagacgc tattgaagaa aagaaagaag aaatgctaga aagcatttat aacttcttag
     1261 ctatctcatt aggaacacct ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga
     1321 actatcatct tgaccgagga ctgacaccac aaatttttta tgataaatac atcggtgtaa
     1381 aattagatga ttatgttagc gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt
     1441 cttatacagt agagatgcta ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg
     1501 ttgatatgga aactttcaaa aaattagcga ttgctcagct tgaagaagga gaatctgttt
     1561 ggtttggttg tgatgtaggt caatcttcta ctagaactag tggcatcatg gccttagatg
     1621 cttattcaat ggatgatcta tttgatacgg attttacaat gactaaagct gaacgtttag
     1681 attttggaga aagtctgatg acccatgcga tggtattgac cggtgttgac ttagtagacg
     1741 ggcaatcaac aaaatggaaa gtcgaaaata gctggggcga aaaagttgga aagaatggct
     1801 tctttgttat gagtgatgcc tggatggacg aatatacgta ccaaatcgtt gtaagaaaag
     1861 atttattgac gaaagagcaa ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt
     1921 gggatccaat gggtgcttta gcatagacga gtatttcaat gaagagtatg tagtaaagat
     1981 aaaaagagct ggcttaagcc agctcttttt atctttaaag tccattccct gttttttatg
     2041 gtccctgtgc tataattcta aataaaggat aaggattgga aataatcctt ttatcattaa
     2101 aaatatgatg acgaagtaga ataaaattgg gagtaggggg gaaagtcatg gatatccaaa
     2161 ctgttttatc tgatctgaaa ttaaatggta aagtgattcc agttgttggt ggcgatgtta
     2221 atcagaccta tcgtataaaa accgaacatc gtgcttattt tttaaaaatc catccgaatg
     2281 taaaaaaagg attttttgaa gcagaagtag acggattaaa agaactgtca gcattcgtac
     2341 gtgtaccgga tacgtatatg ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat
     2401 ggatcgagcc gggaaaaggg gatcaacgag atcttgctgc agctttagct aatctccatc
     2461 agcaaacagc gccacaattt ggttttcgta aagataacta tttgggtaca cttgttcaga
     2521 aaaattcttt tgaagaagat tggtggactt ttttctttaa agatcggctt gaatcccaga
     2581 tttctttggc agaagaaacg aatcgatgga atgtacagcg tcaagaaaag tatctgcgat
     2641 ttaaagaacg tgtattgaaa tcggttgaac caaaaaagat cactccacgc ctcttgcacg
     2701 gagacctgtg gagtggtaat gtgttctttg accagcaagg gcatccagtt tttgtcgacc
     2761 cagctgtttc ttatgggaat cgggaacaag atattgcaat gagccagtta ttcggtggtt
     2821 tccgaccgga atttttagat gcttatcaaa ctatatttcc attagaaaag ggatggaaag
     2881 accgactgcc gatttaccag ttatattatc ttttggcaca tttgaatatg tttggagaaa
     2941 gttacggttc ccaagtagat caattactag aaaattttta agaggagggc gtgtttttgg
     3001 caaaaaaata taccaaaaag catgaagttt catattatga gtgtgacatc aaccaaacga
     3061 tgacatttcc ttcgatgtta ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt
     3121 tgggaagagg cagtgaattc gtcaatagct ttggacttac ttgggtcatc acgaattatt
     3181 ccatggagat taatcgtctc cctagagtcg gtgaagtaat ctcagtaacg actcaagcca
     3241 tggagtacaa taagtttttc tgttatcgga acttctggat ccatgatgaa gatgggaaag
     3301 aattagtcaa aatagaatct gttttcgtat tgatggattt tgtgaatcga aaaatgagca
     3361 gtgttaatga agagatcatt gctccttttg agagtgagaa gatcaagaaa atcaagcgtc
     3421 aggaaaaaat tgaaaagatt gattcgggct tcatgttgcc atatcgtgtt cgtttttatg
     3481 atatcgatag taatcagcat gttaataatg cgatgtattt taattggatc attgacgtat
     3541 taggatatga ctttttgact acgcatgttc cagaaaaagt aattattcga tttgataaag
     3601 aagtagagta cgggaatgag atcgaaagcc attttgaaca aatccctatc gaaaatggtg
     3661 tcaaaacaaa acatgaaatc cgaatgcaag aacagctgta ttgtgaagca aatattatat
     3721 ggaaaaataa ataaaaaaga ggttgtgaca gaagggggca acttcaagaa ataagaagaa
     3781 atttacgaaa attgtttctc aaatttttgt aaattttcga cttagttttc gaagaagcta
     3841 tttttgtcac agcctctctt tttaagttta gctattagat aatgatgttt agttttggat
     3901 aaacgaaatc aattcttcac ccgacaaacc atagtttaca gcaatcaata gttttaaacg
     3961 tgctttttga ctattaatac tgttacagaa aataatcccc attttttcta gttcttttcc
     4021 cccgccttca taatcataaa caggctctgc gattccattg aaacaacgag aaacaagtac
     4081 tacagggatt ttttggacaa gaagcttttc caaagcagct aaggcttgtg gagggagatt
     4141 ccccgcacct aaagcttcga ttaccaaacc gtctactttc gtatgggcga tggcttccaa
     4201 taagtcccct tgcatcccag catatgcttt aacgatcggg atagtcccat ctacagcgga
     4261 aatgtccagt cgctttgttt ctaaaagttc ctgcaagaat aaaattcgat tcttagttac
     4321 taagccgatg ggacccaatg taggtgttcg gaaagtagca acatttgtgg tatgggtctt
     4381 tgtcacgtag cgtgcagaat ggatctcatc gttcatgacc accaaaaccc ccttatctag
     4441 cgcttcttca caacttgcga cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc
     4501 aagttcatta cttgaacgca ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt
     4561 tgtatcaaga aaaaaagcag tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc
     4621 ggatatacct gatgcataag cggacaagat acgttctttt aataccaaca tgtgagaagg
     4681 catgatgtgt ggtgagggaa gattgaagat atcttcaact aataattcta catgcttggg
     4741 gattgtgatt tctgcatcta ataatgggtt tgccacatca ggactaacac cgcccgtgag
     4801 catgttttct ttcatggcga tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt
     4861 acagacttcc ttacgaataa ttttgcatag ccgttaaatc ttattgtata ctatataaaa
     4921 gagttttgct atcagtgaag aataacgaat agcctaactt atcataaaaa agctgacctt
     4981 attaaaatgg aaaagactca accttgacct cctaatacaa tggagtatct ggttgagttt
     5041 cttttacaag aaagcagcca aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc
     5101 ggattaacat cgtttaaaga acatgacaaa ttaactggta aaaagaataa cactctttat
     5161 gaatatgcag ggtttctacc cttagtggaa ttagatactg cctattatgg aattccccga
     5221 aagtcctcag ttgagaattg gataagtgaa gtacctgaat catttcgctt tgtagtcaaa
     5281 gtctatcaag gaatcagcgg tcaaggagac tggaagcctt attatcagac cgaagaagag
     5341 atgattgcgc attttttaga tgttatgtcg ccaatggtag atgctggaaa gatgttttgc
     5401 tttttagtcc aattcccctc atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc
     5461 aaacttagaa agtggtttga gggttatcct gtttctatag aattgcggga ttattcttgg
     5521 tacagcgatg aatttcttga acgtacaaga aactttatgt ctagagccgc tttttcttta
     5581 gccatcatcg atgagccaca gttgctgaat acaacgattc cttttgatcc atttgtcacc
     5641 aatccgaact ttgctctttt tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat
     5701 gataaagagt ggcgtaaaaa aagaacgtta tatcgttatt cagaaaaaga actgcaagaa
     5761 ctagcacatg aagtcaaagt agttgattct aagacaaaag aggtaggtgt gatttttaat
     5821 aataattctg gtggtgatgc ggctgaaaat gcattagcac tgcaaaaaat attaaatatc
     5881 aagccagatg gactcaatcc aacccaaatg gatctgtttt aagggcttat tttattcaag
     5941 aaaggggcga agtctatgat caaagctatt ttttttgata ttgacggaac attggtcacc
     6001 aatcgttcaa aagcgttgga atcaacaaaa caagcaattg aatatgctcg gaaaaacggc
     6061 attttatgcg gtgtagcaac ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa
     6121 ttggaattag atatgtatgt cgtgtataat ggtcaactgg tttttactgc agatcgcacg
     6181 attatcgacc acccttttga acaaaaagta ttggaacata ttgttgaatt tgcagatgag
     6241 aaccatcgac aaatcgtttt tggtgcgaga aatcggttag atggatcaac gacgatgcta
     6301 ttaggacaat cgatttttat caagcgacta gtcagctttt taccgcgtaa attcccggta
     6361 cgtctgatga aaaaaatcct ccaggttttt agccctcacc gacaaaagga tcgttatgaa
     6421 acgttagaga tattaaaaga accaatttat caatgtatat tgctcagtcc ggagtcagaa
     6481 cagaaaaagt tagaacatcg ctttcccgag tgtacgtttc agcgttcaaa tacgtatact
     6541 gtagatatca tcccaaaagg tggttcaaaa ttactaggca tccaagcttt cgcaaatgct
     6601 gtcgaaattg aaatggaaga aattatggca ttcggcgatc attacaatga tatcgagatg
     6661 ctgaaaggtg taggaattgg agtggccatg gggaatgctc agatcgaagt aaaacatgct
     6721 gcagactttg tgactcaatc aaatgaagaa gatgggatat acgaaggact gaaacacttc
     6781 ggtctgattt attagtcaat gaaagaagga aaaaatcagt atggaagcag agacaccttt
     6841 tcaaaaagta gaaacattcc acaaaacttt tgatccaaga agaccagaga tacccacggc
     6901 ttttacagaa aatgaagctc atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt
     6961 tgttgcagct acttcaccta ataaagaaca actgcatgaa gctgttatgc tgctgcacga
     7021 agcattagat aaagcagaag aaaaaatact tggtaaaaca cagtggggaa gtgatccgct
     7081 ggttgaacaa gtagatgcac tgacagatat cctttacttt acttatggtt ctttttcatt
     7141 actaggaata gtgcctgatc gaatattcga aatcgtccat caggcaaata tggggaaatt
     7201 atttccagat ggaaaacctc attatgaccc tgtaacaaat aaagtgttga aacctgaaaa
     7261 ctggcagatg gaatatgctc cagaacctaa aatcgcggca gaaatccaac gtcagattca
     7321 agaagcgatt gctaagaaga aatccgctga tataggcatg tgcacaaatg aaaaagagtg
     7381 atcagagttt cactattttt gttatcttta tacgatggaa aaacaaaaga ttttcgttta
     7441 agttagtgaa tgcatgaaaa tagtatgcta taatctagaa tagatgtctt ttgcacgaga
     7501 gacgtattat ttggatttat gccaagcaga acgccatctg ttgcataaaa gatgcatact
     7561 gaaaacggag gaaccataaa tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat
     7621 tatagtcgcc attgtcgtct ttttggttag ttacttgatg aagaaaaaaa accaagatcg
     7681 attggatgcc ttagaaaaac gcaaggaaga attatttgac ttgccagtga ttgaagaagt
     7741 cgatgatgtt aaaaaaatgc atctggttgg gcaaagccaa aatacctttc gtgaatggag
     7801 tcaaaaatgg aatgacatat ctacttcctc gttcgctgaa ctagaaagtc aaattttcga
     7861 agtggaaaac ctcaatgaaa gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc
     7921 agaagctaca atggaccaaa tggagaaaca agtgacggaa atccgtgaag gtctaaagga
     7981 attgcgggaa acggaagaac gcaactcact agctgttcag caggcacttg acgtctatga
     8041 agaactgaaa gaaaaagtca gagaagacga gcatgaatac ggaccagctc tttttgaatt
     8101 gcaaaaacag atcaaagtga tcgaaaaaga atttacgcag tttgtgactt taaacacttc
     8161 tggagaccct gttgaagccc gcgaggtttt ggataaagca gaaaaacata cgtatgaagt
     8221 ggaagctctg atgaaaaaaa ttccaccgct gtatgaggag ctccatacca ctttccctga
     8281 acaacttgaa gaaatcagtg atacttacga gaaattgatg gaagaacaat atgtattccc
     8341 agaagaagat cttgctgaag atatcgcaaa agtaagtcga agaattgaaa attcattagc
     8401 caatcttgaa aaaacagaag ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt
     8461 gattgattcg ttatatgata tcttagaacg ggaaatggaa gctcagagat atgtaaaaac
     8521 taaccaatca acgattgcag agtatatcaa acatacaaca aagaataacc gtcaattatt
     8581 gattgagctg gatcacacat ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt
     8641 acgaggtttc caaaccgaag tggaagaaat ggaacgtcaa aatgaacaga tgattccaca
     8701 gatccgtcaa cacgaaattc cttattcaga agtgcgcacg ttctataaaa cagtcttcaa
     8761 agtcttagaa gatatcgaaa agcaacaagt agagatcgac gactcattac atgaattgag
     8821 aaaaggcgaa aaagaagcgc aagaaaagat cgatacattc gaattcaaat tgcgcagttt
     8881 gaaacgtttt gtagaaaaac aacgtttgcc tggcttgccg aatgattacc tagaattctt
     8941 ttttgttgct actgaccgta ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa
     9001 catggaagag gtcaatcgtt tagttgctct atgtgaagaa gatctagaat tattagataa
     9061 gaagacacat gatttagtag atgcagcagc attaacggag caaatgctcc aatatgcaaa
     9121 tcgttatcgt catacacacg aagaagtgag agaagcaatt gataaaagct actatttatt
     9181 caataaagaa tatcactatc aagaagcatt agacgaaatc ggtacagcat tagagcgagt
     9241 agaacctggt gctttcaaac gcatcgaaga tttttatttt aatcatccag acttagtata
     9301 aagcaaaaaa gttagtaaac cagcaagagc aagacggatt attgtcttgt tcttgtcttt
     9361 ttttgtatat ttgaaaattg aaacaagtga aaatttcacc tgctcttgaa agcaccttgg
     9421 acaaccgtta taatagaaaa gaatttcaaa aaagaggggt ttcttatgat atattttgat
     9481 aacagtgcaa caaccccaat ctttccgcaa agcttagatg catatgtaaa aaccagtcag
     9541 cggattattg ggaatccatc aagcttgcat gatttaggca atcaagctaa ccggttgttg
     9601 cagcaagcaa gaaagcagat cgctgatttg atccatgtag aaccagaaga aatctatttt
     9661 acttcaggcg gaacagaagg agataactgg gtattaaaag gaacgatgat cgaaaaaaga
     9721 gaatatggga atcatatgat catttcagga gtcgaacatc cggccgtcag cgagacagca
     9781 gaacaattaa aagaattagg aatcgaagta agtatcgccc cagtggataa acgcggattt
     9841 gtacgtgtcg acgaattgaa agaattaata agaaaagaga cagtattagt ctctgttatg
     9901 gctgtgaata atgaagtagg atcgattcag cccatccagg aaatcagtga tcttttggat
     9961 gattttccta aaattcattt ccatgtagat gctgttcagg cgattggaaa agtaccattc
    10021 tcgcaatggt tgacagaaag agttgatttt gcaacatttt cagctcataa attccatggc
    10081 ccacggggaa ctggtttcat ttattggaaa aagggtcgtc gtttagcacc attactgaca
    10141 ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa atgttccagg tattgtggct
    10201 atggttaaag ctttacgttt atcattagag ataaaggaac aaaaacctga gcagacacaa
    10261 cgcttaaaag tagctctaat caatgcgttg aagacgtatg agaaagtgac gattttttca
    10321 gaaggaactg actatgcacc gcatatttta tgttttgctc taaaaggaat tcgcggcgaa
    10381 gttctcgtcc atgccttaga agaaaaacaa atatttgttt ctactactag tgcctgctcc
    10441 tcacgtaaac atatggcaag cagtacattg cacgcaatgc atgtaccaaa tgatctggct
    10501 acatctgctg ttcgaatcag cttagatgaa aacaattcgc ttgcagaagt cgagcaattt
    10561 atgattattt tcaagcagct ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt
    10621 cttgtgaaat acagtgaaat tatggtccgt tatggggaac tttcgacaaa aggaaagaat
    10681 cgaaaaagct ttatcatgca actagcacaa aatgtcagac aagctttgaa agactttcca
    10741 gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt tattgaatgg cgcagacggg
    10801 cagctagtta ttccaaaact agcaaaaata tttgggatcc aaaatttctc tccaagtatt
    10861 cgtgtagaaa aagatgtcga agttttgaaa caagctgttc aagacatcat gaaagaaatt
    10921 tatactggga aagaaacatt taaaattatc gctaaaagaa gcgatcacca atttgaattg
    10981 acaagtaatg aattgaatca aacattaggt aatgctgttt ttgatatttt cccgcatatc
    11041 caagttcaaa tgcgtcaacc agatattcca ttacgtgtag agattcggcg tgatggcgct
    11101 tatctatcgt atgaaacgat caagggagct ggaggattgc ccgtaggaac aagcggaaaa
    11161 ggaatgctta tgctttctgg ggggattgat tctcctgtag cagggtactt tgccatgaag
    11221 cggggagtgg aaattgaagc tgttcatttt gctagtccgc cgtatacaag tgaacaagca
    11281 ctgcaaaaag caaaagatct aacagccaaa ctagcacctt atgtgggaac gatccatttt
    11341 atcgaagtgc catttaccga gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat
    11401 tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt
    11461 catggtttag tcattttgaa tggtgaatca ttaggtcaag tggcttcaca aactttgcaa
    11521 agtatggtgg ctatcaatga agtaacgaat acgccaatca ttcggcctgt agcgacaatg
    11581 gataaacttg aaatcattga agtagcagaa cagatcgata catttaatct ttctatccag
    11641 ccttttgaag attgctgtac gatttttgct cctccacagc caaaaacacg ccctcaactg
    11701 gaaaaggtcc atcaatatga agagcgttta gcaatagatg aaatgatcca acgagcacta
    11761 gctggcttga agatggaaga gatacaagca gaatcgtcta atgaaaaaac tgaagaattt
    11821 tcagaattgc tataagaggt tatgaaaata gtgttttgac cagagtatta gagaaacaaa
    11881 acgaaaaaat agctccatgt attttaccgt ttttgaacta atgtcgaaga ttagctgatt
    11941 gaataccatt tatcaagaag ttatggaaaa gccggttctt ttttactaac tgaaaaataa
    12001 aattttattt agagatcaag ctacagttga taaatgatat aaaaaagaga atttcccatg
    12061 aaatagcgag gttgtgacag aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa
    12121 ttgcttttca aatttttgtg aaatttcggc ttatttttcg aagaagttac ttctggaaca
    12181 ccgtgtatca gaggttgcga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    12241 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
    12301 caccgtttta tttgtctcac acccttttcc ttcctacttg taaccttgct cccgtgttag
    12361 aaacatgtta cactaaatat gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa
    12421 cagatacact gccaaaagca actgcgaaaa gattaccttt atatttacgc tacttgaaga
    12481 tgctggatga ctcaggtata agtagaatca aatcaaatga gttcagcgaa atcacccaaa
    12541 ttccttctgc aacgatccgc agagacttct cgcaattagg cgagctaggc agaagcggat
    12601 atggctatga tgtccctttt cttatcgatg tattcaataa tatccttaat actaaagaag
    12661 aaaaacgaat cgcattagta ggatatggaa atcttggcaa agcattgaaa cacaacaatt
    12721 ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga taatgatcca gcactgatca
    12781 atcgagtaat tgatggagaa atgatctatc cgattgatcg tttcgcggag atagccaaag
    12841 caaaaaatgt gacagtagca atctctacgg taccaagtaa atattcacaa tctgctattg
    12901 atgaaattgt caaaggaaat gtaaccgcta tacttaattt tgcgcctgac agagtaactg
    12961 tcccagctta tgtcaatgtt caatacattg atttgacaac cgagttgcag acgcttattt
    13021 actttgatga gaattactca gaagtttttt cttaggaaaa tttgagtagg cttattgatc
    13081 ttgcgagaac tcgtcgtaat tctttcaaaa attttttagt actaaatgaa gtggtttttt
    13141 cacgaccact tgttgactaa aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa
    13201 gaggagtaac tggcacattc attcttagag agagaactat ctgctgaaag gttctcaatg
    13261 acaaccagtg aaggtagctt gggagtttct gtcttgaatg gagtagagat agacgagtcg
    13321 tgatcgttaa ctcacttgag aggcaataat gattgattat tgcaatttag gtggtaccgc
    13381 gtaaatcata cgtcctagaa tacgaaagta ttttaggacg ttttttagtt taaaggagga
    13441 aaacagcatg acggaagaaa ataacctatc aacaaaatat aatccgcaag aagttgaagc
    13501 tggacgttat cagcattggt tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc
    13561 aaaaccttat tcaatcgtaa tcccgccccc aaatgttact ggaaaacttc atttagggca
    13621 tgcttgggat acgaccttgc aagatatgct gattcgtcaa aagagaatgc aaggatatga
    13681 tactttatgg ctgcctggta tggaccatgc aggaatcgcg acacaagcaa aagtagaagc
    13741 gaagcttaga gagcaaggga tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca
    13801 agtgtgggaa tggaaagaag agtatgcgag ccacatccgc gaacagtggg ctaaaatggg
    13861 cttatcttta gattacagta gggaacgttt tacattagat gatggattat ctgaagcagt
    13921 acgtaaagtt tttgttacct tatacgaaaa aggtttgatt tatcgtggtg aatatatcat
    13981 caactgggat cctcaagcac gaacagctct atctgatatc gaagttattc ataaagatat
    14041 cgaaggcgcc ttttatcata tgagctatga actagcagat ggatcaggcg tagtagaaat
    14101 tgcgacaacc cgtccagaaa cgatgttggg agatacggcg attgctgttc atccagaaga
    14161 cgagcgttat caagcgctca tcggcaaaaa agtgattcta cctttagtca ataaacaaat
    14221 cccaatcatt gcagatgaat atgtagatat ggaattcggg acaggtgtag taaaaatcac
    14281 tccagcacat gatccaaacg actttgaggt aggtaatcgt catgatcttc cacgcgtaaa
    14341 tgttatggat gatgatggta cgatgaatga actagcagga aaatatgctg gaatggaacg
    14401 ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat
    14461 cgatacgatg atccacagtg tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg
    14521 gctgtctact caatggtttg taaaaatggc gccgttagcg aaaaaagcca ttgataacca
    14581 agaaacagat caagcagtgg aattcttccc tccaagattc aatcaaacgt ttttacgttg
    14641 gatggaaaat gtccatgatt gggtaatctc acgacaatta tggtggggac atcaaattcc
    14701 tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg atggaagcac cagcggatag
    14761 tgaaaactgg gtacaagatc ctgatgtttt agacacttgg tttagttcag ctttgtggcc
    14821 tttctcaaca atgggctggc cagatacgga aagcgaagat tataagcgat acttccctac
    14881 tagcacttta gtaaccggtt atgacatcat tttcttctgg gtaagcagaa tgattttcca
    14941 aagcttggaa tttactggcg aacgtccatt tgagaatgtc ttgattcatg gattgattcg
    15001 tgacgaacaa gggcggaaga tgagtaaatc attaggtaat ggaatcgatc cgatggaagt
    15061 aatcgaaaag tatggagcag atgctctccg ctggttctta tcgaatggtt ctgcaccagg
    15121 acaagatgtc cgcttcagct atgaaaaaat ggatgcttct tggaacttta tcaacaaaat
    15181 ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga atgaccgtag aagagatcga
    15241 tttatctgga gaaaaatcag tggctgaccg gtggatcctt actcgtttga atgaaacgat
    15301 cgaacgagta actgagctat ttgatcgttt tgaatttggt gaagcaggtc gacaactgta
    15361 taactttatc tgggatgatt tttgtgactg gtacatcgaa atgagtaaag aagtacttta
    15421 tggagatgat gctgtttcaa aacaaaccac ccgtagcatc ttagtccata cacttgatca
    15481 gatcctacgt ttattgcatc caatcatgcc attcgttaca gaagaaatat gggaacatat
    15541 tcctcatcag ggaaattctc tagtagtggc agaatatcct gttgtacgtc cagagtttga
    15601 tgatgaaaca gcttctaaag ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa
    15661 tattcgttca gaagtgaata caccattgtc taaaccaatt acgttgctaa ttaaaacaaa
    15721 cgatgctaaa atcgatcaat ttttagttga aaatacaagc tatatcgaac gcttctgtaa
    15781 tcccgaagag ttgacgatat caagtgagat cactgcgcca gatctagcga tgtcagctgt
    15841 tctgacaggt gcagaaatct tcttgccgtt agcaggattg atcaatattg aagaagaaat
    15901 caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa
    15961 actttctaat gaacgttttg tttctaacgc gccggaagaa gtagtagagg cagagcgtgc
    16021 aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg
    16081 tactattcaa taagaataga aaaagagagt gtgaaatcag ctagctatta ctaaaagcta
    16141 gctgattgaa catcgttaca ggaaagagtt tgagcctcac tcttagcttc gattcataag
    16201 gagatgtgac ataaattttg tcgcatctct tttttagtct actcttatta tgtaggagaa
    16261 ggagggaggt ggatcatgta tcaagagtca ataaagtagt tgataaaatc gttcctctct
    16321 ctgtttcttt tctctttgtc atacttttat ttttcttatg tgttctattt gttataataa
    16381 aagagaaatg ggggaattgt tgatgacgat tgaagaagca atagaatgga tccatagtcg
    16441 attaccattt ggttcacgtc ctggccttga ccgagtcgaa gctttattag aagtggtagg
    16501 gcatccagag agaaaagtaa aaacgatcca tatcgctgga acaaacggaa aaggttctac
    16561 tgtagcttat ttgagaagcc tattagaaga ggctgggctg acagtaggta catttacttc
    16621 accttatatc gaaacgttca atgaacggat cgcaatcaat ggcaaaagca ttccagacaa
    16681 tgatttgatc cagcttgtaa aaaaatatca gccactgatc aaacaacttg atcaatttga
    16741 agcagtagct ggtattactg aattcgagac attaacagca atggcatttg attatttttt
    16801 gatgaagaaa gtggacgtag cgattatcga agttggctta ggcggcttat tggattctac
    16861 caatgtagct gtaccaatgc tgacgggaat aacaacgatt ggacttgatc ataccgaaat
    16921 acttggagat acaattgaag aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt
    16981 tccagtagta acaggaaata ttgccccaga agcttttact gtcatccaac aaacagctaa
    17041 agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat aaaataaact atctgcatcc
    17101 tgatgagatg tggggagaag tatttgaatt ttttaatgaa ataggaaaga tccctcaatt
    17161 aaccactccg cttttagggc aacatcaagc tgaaaatgca ggagtagcca tcgaattgta
    17221 ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa aaagatgttt tgaagggatt
    17281 gaagaaatct cgatggccag gaagaatgga gcgtttgagt aacgagccat tagttgtttt
    17341 agatggtgcc cataatacgc atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt
    17401 caaaggatat caaatcaaca ttttgttttc cgccttacga actaaaaatg ttagagaaat
    17461 gctagaacga ttgcagcaag taccaaatgt ccatatttat ttgacgacct ttgaacatcc
    17521 aaaagccatc gaattaagtg aagtatcaga tttagcaagt gagagagtat caatcgtttc
    17581 attgtggcaa ttcggattag cagatatatt ggaaaagatg acaaatgaag acgtattagt
    17641 tgtgaccggt tcactttatt tcatttcaga agttcgccat ttattaatac aattaggagg
    17701 agatcatgaa gtataatgca gtgatttttg acatggatgg tttgctgttc gacacagagt
    17761 tagtgtatta cgaagccaca caagtagtag cagacaagat gggttttcct tatgataaag
    17821 acctgtattt aaaattctta ggtgtttcag atgaagaagt atgggcgaat tatcatatga
    17881 ttttctcgga atttggcaaa acaagtgtac aaagattcat agatgattca taccaggaaa
    17941 cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc cggtgtgttg gaactcctct
    18001 cttttttaga agaacatcag attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca
    18061 ttgaactttt attgaagaag aacaatctgt ttgaacgatt tgagacgatt gtttctgcag
    18121 aaaatgttca acgagcaaaa ccagacccag aaattttctt gttagctaat cagaagctag
    18181 gtacagcgaa gaaagaaaca ttgattttag aagattcaca aaatggtatt ttagcagctt
    18241 ctgcggctga gatcccggta gtcatggtac cagatctgct tccaccatcc caagaattga
    18301 tcaaaaaaac agctggggtt gtttcttctt tacatgaaat ccctgcattg ttaaaataaa
    18361 aatccatctc ttttcgtatc ttattttaga taagatagaa aggagatgtt tttttgggaa
    18421 aacgattgat caaagaagta cctactagct ctttaccaag ggaacgtatg gaaatttatg
    18481 gcgcggaagc tttatcggat caggagcttt tggcaatctt actgcgtacc ggtcaacacc
    18541 cttatagtgt gatgtcgatt gctggaaatc tgttaaaaac gtttggagga ctggcctctt
    18601 tacgacaggc tactttgcat gagttagaag agattcgagg gataggacgc gtgaaagcaa
    18661 tcgaaatcaa agccttgatc gaattaggtc gacgaattca gacggatcat gaaagagcgg
    18721 ctccaccggt tcgtaccagc tacgaattgg cccagcaatt gattctggaa atgaaagatc
    18781 acaaacaaga acatttggtt tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa
    18841 aaacagtgtt catcggttcg ctgaatcagt cagtcgctca tcctagagaa attttccatt
    18901 atgctgtacg ttattgtgca gcaaggatcg ttttagcgca taatcatcct tctggtaatg
    18961 ttattccatc gcagcaggat atgaatttta ccaaaaggat acaaaagtgt ggagaaatga
    19021 tggggatcac tgtactggac cacttgatca tcggacgcaa acgatatttt agtttgagag
    19081 aagaaggaat gatggaagag aagtaagttg ctttcttgca aaaaaaatga agcaagtgta
    19141 caattatatt gtaattttgt ttgggaatag aagttgaaac atctgttgca cctctatcgt
    19201 acacgagcaa tgttacaatt taaattagtg cttttggcac gaggaggata ttattatgga
    19261 aaacggaaca gtaaaatggt tcaacgctga aaaaggattt ggatttatct cacgtgaaga
    19321 tggaagcgac gtattcgttc acttctcagc tatccaaggt gacggattca aaacattaga
    19381 agaaggtcaa gcagtgactt tcgacgttga agaatcagac cgcggcccac aagctgcaaa
    19441 cgttgtaaaa gcttaatttt atctgtaaat caaattgatg gtagacatca atttttacga
    19501 gagaacaaag cgagtaacaa aaat
//
LOCUS       DADXTH010000059        19427 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.059, whole genome shotgun sequence.
ACCESSION   DADXTH010000059 DADXTH010000000
VERSION     DADXTH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.059"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IWI97_001973"
     CDS             complement(451..1761)
                     /locus_tag="IWI97_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBC2463468.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IWI97_001974"
     CDS             complement(1773..3191)
                     /locus_tag="IWI97_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2463469.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IWI97_001975"
     CDS             complement(3431..4999)
                     /locus_tag="IWI97_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBC2463470.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IWI97_001976"
     CDS             complement(5003..5413)
                     /locus_tag="IWI97_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBC2463471.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IWI97_001977"
     CDS             complement(5410..6333)
                     /locus_tag="IWI97_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBC2463472.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IWI97_001978"
     CDS             complement(6330..7208)
                     /locus_tag="IWI97_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBC2463473.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IWI97_001979"
     CDS             complement(7195..8532)
                     /locus_tag="IWI97_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBC2463474.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IWI97_001980"
     CDS             complement(8535..9638)
                     /locus_tag="IWI97_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBC2463475.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IWI97_001981"
     CDS             complement(9712..11220)
                     /locus_tag="IWI97_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBC2463476.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IWI97_001982"
     CDS             complement(11186..11878)
                     /locus_tag="IWI97_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBC2463477.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IWI97_001983"
     CDS             complement(11900..12874)
                     /locus_tag="IWI97_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2463478.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IWI97_001984"
     CDS             13174..14046
                     /locus_tag="IWI97_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2463479.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IWI97_001985"
     CDS             complement(14504..16702)
                     /locus_tag="IWI97_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463480.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IWI97_001986"
     CDS             16934..17926
                     /locus_tag="IWI97_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2463481.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IWI97_001987"
     CDS             17986..19407
                     /locus_tag="IWI97_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBC2463482.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
BASE COUNT         5818 a         4012 c         3244 g         6353 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatga
//
LOCUS       DADXTH010000060        17685 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.060, whole genome shotgun sequence.
ACCESSION   DADXTH010000060 DADXTH010000000
VERSION     DADXTH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.060"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            195..494
                     /locus_tag="IWI97_001988"
     CDS             195..494
                     /locus_tag="IWI97_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463483.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(591..1025)
                     /locus_tag="IWI97_001989"
     CDS             complement(591..1025)
                     /locus_tag="IWI97_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2463484.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1257..1967)
                     /locus_tag="IWI97_001990"
     CDS             complement(1257..1967)
                     /locus_tag="IWI97_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBC2463485.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1957..2817)
                     /locus_tag="IWI97_001991"
     CDS             complement(1957..2817)
                     /locus_tag="IWI97_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBC2463486.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2822..3460)
                     /locus_tag="IWI97_001992"
     CDS             complement(2822..3460)
                     /locus_tag="IWI97_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBC2463487.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3475..3774)
                     /locus_tag="IWI97_001993"
     CDS             complement(3475..3774)
                     /locus_tag="IWI97_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2463488.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(3817..5301)
                     /locus_tag="IWI97_001994"
     CDS             complement(3817..5301)
                     /locus_tag="IWI97_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBC2463489.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5321..5782)
                     /locus_tag="IWI97_001995"
     CDS             complement(5321..5782)
                     /locus_tag="IWI97_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2463490.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5800..6867)
                     /gene="ulaG"
                     /locus_tag="IWI97_001996"
     CDS             complement(5800..6867)
                     /gene="ulaG"
                     /locus_tag="IWI97_001996"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBC2463491.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7085..7855
                     /locus_tag="IWI97_001997"
     CDS             7085..7855
                     /locus_tag="IWI97_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBC2463492.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8128..8955)
                     /locus_tag="IWI97_001998"
     CDS             complement(8128..8955)
                     /locus_tag="IWI97_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2463493.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9522..10757
                     /locus_tag="IWI97_001999"
     CDS             9522..10757
                     /locus_tag="IWI97_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBC2463494.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11756..12016)
                     /locus_tag="IWI97_002000"
     CDS             complement(11756..12016)
                     /locus_tag="IWI97_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBC2463495.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12043..13344)
                     /locus_tag="IWI97_002001"
     CDS             complement(12043..13344)
                     /locus_tag="IWI97_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2463496.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(13636..14094)
                     /locus_tag="IWI97_002002"
     CDS             complement(13636..14094)
                     /locus_tag="IWI97_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2463497.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14107..15437)
                     /locus_tag="IWI97_002003"
                     /pseudo
     CDS             complement(14107..15437)
                     /locus_tag="IWI97_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(15448..16890)
                     /locus_tag="IWI97_002004"
     CDS             complement(15448..16890)
                     /locus_tag="IWI97_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2463498.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(16877..17641)
                     /locus_tag="IWI97_002005"
     CDS             complement(16877..17641)
                     /locus_tag="IWI97_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBC2463499.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
BASE COUNT         5579 a         3682 c         2732 g         5692 t
ORIGIN      
        1 aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt ataccatatg attatttcaa
       61 tatgtatcct ggaattggag atctcgtaga gctatatcaa gatgaatcaa atatacttgt
      121 cattcatcat gaaatataga gacttataaa attgtttatt tttataatgt atcagtatag
      181 aaaggaaata aaacatggat tatactgttg aatttgacag tgaactactt aagcaatttc
      241 cacaagaaga acatttcatg tataaagaag caatttccca caaagtgaca gagtatttgg
      301 agcataattt ttttcgtata aagccagtgc gtactcgttc ggcaaaagga atatacgaga
      361 tgaaagtcaa aattaataaa aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg
      421 tgttcatttt ttatattagt tcgaatttgc aaaaaattgt atttgataaa gaggtgagta
      481 aaaaaataaa atagctgagc attggaggat agattgagtg atactaagtc tatcctcttg
      541 tttttctaaa gaactaagct agaatttttt cttttgtacg ttattatatg ttatttgtca
      601 ttaccaaaaa gcaccttttt tcttctagta tctatgacat aagaaatacc acccatttcc
      661 tttacttgaa caaatcctac tgattcataa aatttttttg ttttctcagt atcatctgtc
      721 aataattcga tttggactgg tccataacta gtgatcttct ccaaagcttc attcaaaagc
      781 actttgccta ttccttggcg ctgatacctc ggatgaatga ggatatcttg aataaatgcg
      841 atcgtcgcaa aatcagtgat ccaacgaacg attccgataa tcttcccttc cccattacgc
      901 tttatcagag attcagattg ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa
      961 ttcgtccaac caacgctctt atataattca gaaatctcat cgatcataag ggaatcaata
     1021 ttcatttttt tcttcctttc ttatacaatt ctccctctaa taatacagtt ctttgactat
     1081 ttaatctatc acatataact ctcctgtaaa gattctctcg attccttaaa atctctcttt
     1141 ccttttagaa tgcatccttt ttgataaata aaaaggttgt gataaatttt cttcacaacc
     1201 tctgtttctc tctatcaagt gttatctttt aattttctac ccttacccta gcatctctat
     1261 tgcccgtagt atgcatactt tccatgtttg cgaaaataat gtttatctag taaatattgc
     1321 gggatttcac atatattggg gttgatcagc tcgttttctt tggccatcag acaaatttca
     1381 tctaagatca aactgttttc tactgctttc ataggtgaat ccccccatgt gaacggtcca
     1441 tgtccataga caagtactcc agggacttcg tttggatcta attttctttc cttaaacgtc
     1501 tcgacaatca cattccctgt atgaacttcg taagcttctc ttacttcttc tttcgttaat
     1561 tggcgtgtac aaggaacctt accataaaaa gcatcagcat gagtcgtgcc ataagctggc
     1621 aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg agtaatagca
     1681 gttatatctt caaaagtttg atacaaaaca acatgtgtgg ggagatctga agatggtttt
     1741 aagctatctt cttctattat atttcccgac aaatctgtta ccaccatttg atctgcttgc
     1801 atgcattcat atctgacacc gcttggctta atgacgatga ctcctagctc acggtccact
     1861 tcactcacat tgccccaagt caatttgact aatccgtatc gcggcaatgc cagatttgct
     1921 tcaaggacac gttgcttcat tgctgcaatt ttttcttcac aagacatcat agccagcctc
     1981 cttcaattta gaacttaagt agtcttttgc ctgctggatt tctcttcgaa agtcttggct
     2041 tttttcactc cacatttcaa tcagaaatgt tccatcataa tctagacgtt tcaaatgtct
     2101 taataatcca agaaaatcaa cacaaccgct accaaaagga acatcacgaa actggcctga
     2161 atattctttt gttacagggt aagtatcttt taagtggata gcagtgatat aatcgatccc
     2221 tttctcaagt tctcgagccg gattattttc cggccatgcg gacaaattgc ctaggtctgg
     2281 atatacttgt agaaaagggg aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga
     2341 gttcataaat ggatcatcca tgatttccac ggaaagaatg atgccatatt tagaagcttc
     2401 ctttacgctt tcttttattc cttcgataaa gtcttctctg gaattcattg atttctcttc
     2461 ataataaaca tcgtatccag ctatttggat tactttgacg cttagattat gcgccaaatg
     2521 gattgctttt ttcattagtt gtttcgctgc ttttttcttt tctggatcag cagaaccaaa
     2581 cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg agctgaacat
     2641 ctcttctctc agctcagcta tcttttcttc agaccaatct aatcgtgcta accgctcatc
     2701 cgtctcatca atcgacatct cgataaaatc gaagtccatt tcttttacaa gggaaaaacg
     2761 ttctttccat gaaatatctt tgggtaatgc tttttcataa atcccaattt gagccatttc
     2821 cttaccccca gatacgaatg atctcatctt tgaagttttg tgcggccttc atcggatcat
     2881 ttgctgctgt aataccacga ccagtgataa aggtatacac atcgatccct tcaaataagt
     2941 gtaatgtgtg tggatttaat ccaccagtta cagaaacatt aaagcctagt tcaatcagtt
     3001 ttttgatctt cgataaatct ttttcacccc aagtttcacc agataacagc gcatcacggc
     3061 tttgatggta gattacctgc cggataccga tgttatgcca atccatggct tgttcaaaag
     3121 tccaattgcc gtagagttct acttgcaatt cacctatttc tttttgtgct gcctccatag
     3181 ttggaatcgt tgcacaacaa atgactgtca tccagtctgc tcctgctttt gcaacattac
     3241 tcgccactgt tcctccagcg tctgcacatt tcgtatcagc gactagtttt ttatctggga
     3301 acatactgcg aatacagcga atcgcttttt gtccttcctg taagcaaaga attgtgccca
     3361 cttcaataat atctactaca tcaccaactt tcataacatc tcctaatgca tgttctaagt
     3421 cattgtgatc taacgcaatt tgcaaattcg gcctgctcat cttagttcct tcctttactt
     3481 gtttttttgg taccactttg tttcagcaaa tttttcactg atttctttgg ctgacatcac
     3541 atttttgata ccgataactg taacttcttt tttctctgcc tgagtaaaca tattcaaaaa
     3601 attgactggc gtaaaaacta catcgtattg gccagctgaa ctttttccct ctgaaatggc
     3661 acaatgatgg attttggtaa ttggtacgtt tagttctttc attgcttttt ccacacttct
     3721 catcatcatc aagcttgttc ctgatccatt tgcacacgat actaaaattc tcatcttaat
     3781 ccctccatta tgagtgttgt ctaatcagat tttcttttat actctctgct tttctgcata
     3841 tgtgtcatag tcttctacaa tcaaaaaata accctcagga tttttccgat attgtaactg
     3901 cggaatagcc aataaaccaa tgacaactac ggcaattcca gcataaccta ggactttcat
     3961 gattactgta aacaatggcc aagctgttgc ccagtcgaac atcccgatat aaccaccata
     4021 tgttgacatc ccaatccatg tagcgaataa tgctgaaccg cctacttgta tgattcctga
     4081 aagaaacggg aacaggcaag cggctttgaa tccacctcgg ttattggcat aaactgctat
     4141 aacggcatta tcaaagaaca acggaatgaa accggctatg acgatcgttg gtgatttcaa
     4201 taaaatcagt aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga acccaatcgt
     4261 taccgcattt ggcgaaccaa agccaaaagt agcagctacg tcaattcctg gaacagctcc
     4321 tggtaaaatt gtgtttgaga ttccttggaa agattgagtt agttcatcga cgaatgtccg
     4381 aacacctaat tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg tcataatgta
     4441 gaataagaaa ctctgtcctt cttgcatgaa ttgcgcttga atcaaataat ctttgcctag
     4501 tacaatcaga atgatcccaa agaaaaatag catgagtaac gaagtagcaa ccatattctc
     4561 attgaaaatc gacaagaatc ctggtaattt gatatcttcg atttttttgt cttgcttgat
     4621 tgatctattt ttcttttcat cccgcttttt catccactca gctaattttg caaatataaa
     4681 gattccgaac atttgctgat gtgccactgc aaatccggca ccttctgtca attcctgcgt
     4741 gattcctact gttaagttag aagctactgc ccaataacaa cctaaaatca gtcccataaa
     4801 aatcaatact tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt
     4861 agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt
     4921 ctgaaaacgg actaataaga tatttaaaac aaatgcaatc agtaataaaa tcatcacatc
     4981 tccaaaggta cggccaaatg tctccattaa tccagcatcc acagcatttt gtccaaaata
     5041 aggatctgta accatggcat ccaaattgaa ccgttccttt agtccaacca aaatcggccg
     5101 aaaattattg accaaaccac cagagccaac agtcaaaata aagtaaccga ccgttgcctt
     5161 taagaatcca gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc ctaataacac
     5221 gataaaacca atcaggtatg ctggctgcgt caagatattt gttgcaaaat acgtccaaat
     5281 gcttaacaaa aattccccca tcttttttct cctctatcaa ttaattatat tctgccatca
     5341 caacttggta atcatcgatg gagttcgtag agatgagacg ctcaaccaat ccatctgtca
     5401 ttaataattc ggataatttt tggatatttt ctagatgttc ttctggatct tttgctgcaa
     5461 gtgtaaaaaa caaactagcc gttttctcct catcctcccc ttcactttca aaacggattg
     5521 gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac accatcactt ttttctgaag
     5581 aatgcggcat tgctacttga ggtgcgatca caatataagg accgtatttt tctacacaat
     5641 ggatgatctc ctccacatac gtttcatcaa taatttcctt atcgatcaat acaagacagc
     5701 tttcggcgat agcttcacgc caatccgtga tttctttatc tgggtaatga atcaattgat
     5761 ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct tataaaaacg acggaaacgg
     5821 tgtatcattt tcaatcgaga agacatcttc aaatcctcga taaaaattaa actcaagatc
     5881 atctttgttt gtcggataga tgaatttccc gcctacttgc cagatataag gtttaaatcc
     5941 atacttcagg cgatcctttt tgaatttcca aatttcagtg atctcttttg aatcagccat
     6001 aaaattagac caaatatcgt agtggacagg aatcactact tttgcattca atgactccgc
     6061 catgcgcagc atatcaacag aagtaacttt gtctgttatc cctcgtgggt tttcaccata
     6121 ggcccctaaa cagacatcga ttttgtgttc attaccatgt ttggcaaata aattggagta
     6181 atgtgaatct cctgcatgat aaagattccc tcctgatgtt tcgaataaat agtttactgc
     6241 aatttgatcc atctcctgag gcatttttcc ttttaatacc tcttctggat cttcacaagt
     6301 gaccaacgct gtccgatcaa acgcttctaa agctactaca ttgatgtctt tgattttgac
     6361 tgcatcacct ggctttactg taatcgtttt ttcttcagga attccccatt tttgccacgt
     6421 gcgaaccact tcttttggcc caatgaatgt tgcttcagga caattttgat gaactgctgc
     6481 ggctgtatgg atatctagat gatcggaatg gatatgtgta acgattaaag cgtctacatt
     6541 ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt aaattaggct gcatattttg
     6601 aacgccactc atacgcatca tctgatggcc ttttttcatc atgccattcc catgactacg
     6661 ttttcccgtc ccacaccata gatcacataa aatattcgtt ccttcatgac ttttcagcca
     6721 aattcccgta cagcccagcc accacatagc aaaagttcca ggatccacta cttctgtttc
     6781 gatttcttca ttcaagtatg tgccccactc aggaaatgtt tctactaacc acttttcttt
     6841 tgttacttca ttgacagttt tactcatttg cttgctcccc ccatatcgtt gttccaactt
     6901 acaagtaaat attaatgtgt atcagcatcc atggaaaccc ttttatatga ccgtttgttt
     6961 tttatatgat tttctttcag taatcaatga ttgtttcagt ataataaaga cagatatgaa
     7021 tgtttaaatg aacaactagt caaataaatc catggatcaa gatgtccctc aggaggcaaa
     7081 agtcatgaaa aactcacttg tcaatattga aaaacgtcaa aaagaaattt tggaactgct
     7141 tcagcagact gaacaactat caactttcga attagctgaa tcccttgatg tatctattag
     7201 tacgatacga agagatttaa atttattaga agaaaaaaac gagatcatac gcaattacgg
     7261 ttattgttct tataattaca aaaaccagac tgattttgat caatctggtc ctgaaaggat
     7321 taaacaagcg attgccagag aagctagtaa atacattaac gattatgata cgctgtttat
     7381 caattctagc tcgactgcgt tgaaaacact ggattttctt caatctaacc atttgacagt
     7441 cattacaaac aacgtgaaaa tcaattactc tccgcacaaa gataattgta gttatatttt
     7501 aactggtgga gagatgcgct ttccaaaaga agcactagtg ggcgacatcg cattaaatac
     7561 gatttcttca gtcaatgcag acatttgtat cattggctgt agcggagtcg atgtagtaca
     7621 tggggtcaca acaaagatat taaatgaatc aacgatcaac gaaatgatgg tcaacaagac
     7681 aatcaaatgt aagattttag tcgctgatca tcgaaaaatc ggactgacat caaagttcaa
     7741 gattgctgat atttcagtct ttgattattt gatcactgat aaattttgtt ctgaaaaagt
     7801 attagaggag atcagaaata cggggataaa aaccattcaa gtagattcat tttaagaacc
     7861 aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat aacaaattta tcgataaatg
     7921 aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga tgtgtccatc caatcgatac
     7981 catcataatc tattgcttct tttataaccg ttatatgttg cctaaatagg aatcaaacaa
     8041 tatcgaaaaa aggaggtatg acaaaagtca cacctccttt tttcgaataa acggtattca
     8101 aatcagaaca ctgatttcac aaccacttta tttcaggtcg ttttcaatca cctttaccgc
     8161 gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt ctaaataatc
     8221 atcttctcct aattgcttat ggcaagaagc aatttcataa agcaagtatt ccaatgcata
     8281 agtcgattta tgtttcttat tatactcaat acccttctca cataattgga ttgctttatc
     8341 ataatcctcg attttacggt aataggtggc gatatcataa tatacaatgc agaccatcag
     8401 attatcaaaa tcagtctcac tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa
     8461 atctaattct ccttttgaca agtagatcat tgccacgttt gcagctgctt gtaagtgatg
     8521 gatatctgtt tcggtcgttt gttccaacac ttgattaaaa taaaataatg ctgcatctgg
     8581 ttttttcgca taaagatgat caagcagtcc ttgataaaat aaaatttgat ttaaaagatc
     8641 agaattactt actttttcct gatcgatcgt ttgtaaaatt ttataagatt cttggtattt
     8701 tgatacagaa ttcaatgatt gaatctcatt tatttttttc ctgattttgt cttcttctgt
     8761 ttctactaat atatcggaga tctcgacatt cagctttttg caaagcgcac ttaagatatt
     8821 gatgcctggc gacatattct tgttttcgat attgctgaca gttgcttgtt cacaaatgcc
     8881 ttctgctaat tgtacttgtg ttaacccctt tttctttcgg tattctttta gtaatgctcc
     8941 atctacttta atcaaaacaa tttccttccc tttaaaaaat tatttctcct ttatatcata
     9001 cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca gaataaaaat tttaatttta
     9061 aaataatatt caaattaaac aactggcgta tctgctcctt gatttctttt tgtgttatct
     9121 gaataattat cgtttacagt tataacacat agtgaaaatt gtcatggcga gaaagtctaa
     9181 gaatattttt aaacataact atttctttct tacgtgtgta taatgacgac agcacaccta
     9241 tcaattaata taattatttt attttttatg ttctcatgtt tatttttaat gacacaactt
     9301 ctcgtattta tctggcacaa catataaatc gatcaaaaaa ttgttgcgtg tttttttgaa
     9361 aattggagtt tcaacttgat tacaaaattt aaatttagaa gttgtgaaaa cagcgagaga
     9421 cagtttaata ttcaaaatat acataggcta ctcgaacgct gtgtttttta gtatactagt
     9481 aagaaacgaa gggatagact cattccaata aggaggattt catgaagaca attctttttg
     9541 ctcctgcagt attcaattta gctgagacaa cacgaatgat tgaggttgcc aaagctatga
     9601 aagaccgtta ttactgtgta ttctttggct tttctaaaac ttttgctcct ttgatcacag
     9661 aaagtggctt tgagtatcat ttactagcgc cgatccttac gaaaaaacaa gaagaacaaa
     9721 tcataaaatt tgaccagttg cgtacactta aaaacccttt taccaaagaa atggttgcct
     9781 ctcgtatcac tagtgaacag agtctgattg ctaagtttga gcctcgactg attgtgacag
     9841 gttcgaatgt gacaattttt ttatcggccc gcaataaaaa gattccttta gtatatgtta
     9901 aaccctatgc tttgagtgaa cctttttttc aatcagagca tgcgctgatg ccccacgagt
     9961 tacgtttctt cggcccttta agcaatcccc tttggcactg gacaaaaaag cgtatagtaa
    10021 atacccgatg gatccccaat gccttcaaac acgctgcaca aaaagaaatg cttcctttac
    10081 caaaaacaag tttacaaatg atggatgctg atctaaatct catcacaacg cctaaactat
    10141 ttttggaaga agaacagctg ccggaaaatt atcgaatcgt tggtccaatt tttgctaagc
    10201 tcagtacttc cttgcctgaa gaagtgctaa cttttataca gcgaaagcaa caagaaagaa
    10261 aacgtattat ctatttcgca atgggtagtt caggcaatcc gcgtatgtta cgcaaaattt
    10321 tgttcttctt acaaaaacaa ccggatctag cggttattgc acctattgac tatctgcttg
    10381 atgacattca atctgactca gaagatcttt taataacaga atatctgtct agtcatcttt
    10441 tgcatgaatt cattgatttt tcttttatcc acggcgggga agggaccgtc caaactgcct
    10501 gtgaatccgg caaaccgttt atcggtgtgg ggatgcaatt tgagcaaagc tgtaatattg
    10561 actattgtgt aaaatacgga aacgcaatcg ccctcacaaa accagtgact gaaagaaaac
    10621 tgcagattgc tctgcgcgaa gtttcttcac ccaagattcg tcagcaggca cgcacgctgc
    10681 aagccgtttt tccaataaat ggtccacaac aagcaattca agaaatcgaa accttcctga
    10741 gcaaccaccc aatatagttt caagtagaaa aagctttttt aatagagact catcagtaat
    10801 ttcgctattc gagacccaga cagtgaaaat acaaaaaaag taaatagtgg attgaaaagt
    10861 tagagttact taaattacgg tgtctaaaaa acacaaaatc atccttaaaa taaaagatct
    10921 gacgcaatcc aaatgagcat aggtagcgtc tgccatttat tcttagtcaa attctttgac
    10981 acctttctct ggttggatca tctcaacgtt tcttcaaaag aaagaccata ataattaggc
    11041 aaatacattt tcttaaaatg tgtcatatag atctagaagc atcgctttgt agagatttgt
    11101 gctgctagtc ccttcaacaa ttactaaatc aaatgtagga tttaccactt tatctttgat
    11161 aatcaacctc cctcttctgt tgagacaatg atctcgcaat ccgatgcata ctcctatata
    11221 aactgccagc aatccccaca tgctgagtag atcatgtcag aatgttctat gtcgtcagca
    11281 acgataaact aaaagtcacg ttcaccatga ctgattgcta ttcccagcgc aattatttct
    11341 atacacgttt catgatttga ataaacactc tcatcataca tattaccatt catacaccat
    11401 actgcagccc ctactatatg gttgtaagct cccaaatcta tatttgcctt gatcactttg
    11461 gtagtagatc gactaattct taatccattg ctgataaaat tttcattttt gcctccttct
    11521 ttctgccaaa acagttaaaa caataataat ctttatgtaa aaaagggctg tgacaaaagc
    11581 cttgtcacag tcccatattc cgaataaacg gtggtcaaaa gcttgcatct gcggtaataa
    11641 gttcaaccaa acgaaaaata agccaacatt tcacaaaaat ttaagaagca attttcgtaa
    11701 atttcttctt atttcttgaa gctaaccact tctgtcacaa ccacttttag aacatttaaa
    11761 gcttcgatgt gacaaaccat ctatgctgct cgatcataaa attgatagca aaaattccac
    11821 aaagtacata tgtcccccat gaaaacaaaa cattcatatg cggtacaatg atctttggaa
    11881 gaatcacgat caaaaatgat gccagtgccc aaatccatcg attgatttga atatgatatg
    11941 tagctagcat attcaatata aggtgactag ccgttaataa aaagataaaa acaaaaaata
    12001 aaatcagcca atacattgtt tcccaccttc taaaaaccaa acttagacag tttggacttc
    12061 gttttctcca cgactttgtt catctgacag catcttttta tcataagctt ttacaaatgg
    12121 ataatagata ataaatgcta tcaatgtgtt gatcatgatc aagataacaa cacgccagtc
    12181 atttcctgct gaaagataac cgccgattgg agctggtaat gtccaagttg gcaaaacagt
    12241 aaatctattg accatttcta atttgactgc aaaataactg ataatagtca aaactaaagg
    12301 aactaagttg aacgggatga acatgtaagg attcaacatc ataggagctc caaaaatcat
    12361 gggctcattg atattgaaga taccaggaat aatagaaaaa cgaccaacct ctttcaagaa
    12421 ttttgattta cagaagaata taaacaaaac agataccacg atcgttgctc ctgcaccacc
    12481 aattgttacc gtccattgat aaaactgttc aggagcgata tagggtaatt gggtggcagc
    12541 tgtaccatct gccaatgcct ttgcattctc atccaacatg atcagccaca ttggacgaac
    12601 aacagcccct acaatactca tgccatggat accaaatgac cacaacaaat ggatcaaaaa
    12661 cattggtagt aaaacaccaa gcaaattatt ccctaaaata ttggttaaag gctcgaataa
    12721 cgataataag gtagcgttta catcaaagcc cgcgacgctt cgaatcagcc aagtaattgt
    12781 taaaatgact gcacctggaa tcaatgcctc aaaactacgg gcaacagcag gtggcacttg
    12841 ttctggcatt ttgatagtaa tatttcgatt tttaaagaaa cgatatactt ccactgcaaa
    12901 aatcatagat aagatccccg caaacatccc agatgctccc aaagaactca taggtaagac
    12961 ccagccaaga ggtgcctttt caggagttaa aacgtttaca ggaacttgta acataaagaa
    13021 cgttcctaaa gataatatac cacctgtcac actatctaat ccatatgatt tagctagatc
    13081 cgaacccata acaaaagttg cataaatagc catcaatccc atcgtcatcc taaaaggtaa
    13141 aacaatcgtt gccgaataag gggcaataaa ttcattccat ccaggaaacg gtaaattgat
    13201 aaagaccata aaaaatgtcc caatcaaaat caatgacaat gttgaaacaa ccccattacg
    13261 aattgctaat aaatgacgct gtgtaccaat ttttgccatt ggtgggacaa gtttctcttc
    13321 gacccatttc atcagctttt ccactaaaac cactcctttt caataaaata tgaaccattc
    13381 attgcatgac aacgcaaaga atgttcatga ctaattatat aattaagaaa ttttatttca
    13441 atatattctc ttaaataatc aaatttattt ttaagaatcc tgaaaataag ttttctaaat
    13501 cataggagca acaaaaaaat tcgataatta acaaaaatca aaatgattaa atctagtgat
    13561 tgaaacttat catttcaaaa aaattttgta aattcaccca taaatatcac cctatttaac
    13621 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
    13681 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
    13741 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
    13801 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
    13861 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
    13921 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
    13981 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
    14041 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
    14101 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
    14161 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
    14221 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctgctgcaa cttgtgcctc
    14281 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattgtct ccacaccagt
    14341 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
    14401 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
    14461 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
    14521 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
    14581 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
    14641 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
    14701 ggttttttgc caagttttgt ctcctatatt aaaatcggcg acttgcattt cttttgttat
    14761 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
    14821 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
    14881 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
    14941 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
    15001 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
    15061 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcctgtc ctactaatcc
    15121 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
    15181 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
    15241 aatatttgaa aaaccttttt tttcagcata aatttaacaa tcaagtacat atccatattt
    15301 ctttcttcat caatatcata taaacgtatt tcagataatg gcaaccgttt ttcattgttc
    15361 aaaatggctt gaacgattcc tggtgtatac gtactcccgc ctccagctat agtgataatc
    15421 tgttttttca tcatcatctt ttcctttcta ttcttctaat aatttattga attctgccat
    15481 aacataagtc acttctaagc cgatgataat ttgataattt ttttcagttg gtttaatgat
    15541 cccgcttatc cctaattgct tgattcgctg ttcctcgacc atggtgggat ccactacctc
    15601 tacccgtagg cgtgtattac aactatagac atccaggata ttggcttttc ctccaaacgc
    15661 ttgtagcatt tttgttgcca tatattcata atttttactt gctaacccta actctgtttc
    15721 ttctaaggtt acttcattgc caaactctgc cttagcttca cgtcctaatg tctgaatgtt
    15781 tctcttattg atcaaataat aaaaagtaac gtaatacaag gcaaaaaaga aaattccgat
    15841 tggaagaatc aaaatagcat tggtagcgat tcgaaaattg atgagataat caacaataca
    15901 tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc
    15961 ggcaaaaagt gcatggatca cgtacaacat aggagaaaga aacatgaaac taaattcaac
    16021 aggctcagtg atatttgcaa taaatgaagt tcctgcaccg cttcccatga ttcctttgac
    16081 catctcttta ttttctttat aagctgcttt atagatagct agacatatag ctggtatacc
    16141 aaacatcatt actacaaaaa aacctgataa aaacagtcca gcctgaggat ctccgttgat
    16201 aaagcgtgtc atctctcctc tgaaaacttc cttgctagga gatgtatagc ttcctaaatc
    16261 gtaataaata tacgtattca acacatggtg cagtccaaag gggatcagca aacgattcaa
    16321 aaacataaat atccccacac ctaatgcccc catatcaacg agcagttttg caaaattatc
    16381 aatcccaatt tggatgagcg gccagataaa gcccaaaaaa atagctgcga ttgtttgtag
    16441 aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa
    16501 tttttgttct ttaaagcgat tatacgtcca tgccgctact aaaccggaaa taattccacc
    16561 aaaaatcccc atattcactt ctgggtccat gatttctaag ccttttttca aggtagcaat
    16621 cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta tttttgacaa aaccgattgc
    16681 aactcctata gcaaataaga tatccaagtt tccaaaaacg gcatttccag ctgcaccaat
    16741 tactgggatg ttcagcatat catcagctga taaacgcatg atcaatcccg ctaatggcat
    16801 cgctgcaact ggaagtagca tcgctcttcc aagcttctgc acatttgatt taacattcat
    16861 ttttattcct cctctattaa atatcctcat atgttactaa ctcaatcaaa ggatcatttt
    16921 tcactttttg agcaaatgct gaatgaatta aaaattcgac ttggtgagta cgaggataca
    16981 caaaacttga accatttaac aattcctgat ccaaatacgc tgcgtgacac atgacttcga
    17041 tcgttttatc ttctctcaat atcatgagta aacttgccaa atattcttcc acactcgtat
    17101 tgatttgtcg ttttttttca tcagtttctc ctacgacatc gaaacaaggc tcaaaataat
    17161 cgacatgacg aacttcatct tttcgttcaa agttgctccg aaccggcaaa tcatattttt
    17221 ttgctaatgt aacaactacc tcctggtttt catccaaagt atgagtatga tgatgactgt
    17281 ctaaatgagt tggtatgatc cccgcatgat aaattttttg aatctgagca ttccattcct
    17341 gatacagttg atctttgtca atgttaaatg gatgctgata ataagataag gatctgaact
    17401 ctccgctttc gataagagta tcgacagttt tgagaagtgg tttgccacac gtcaatgtta
    17461 aatggactcc tacacctagc ctaggcattt cctttcttag tttcacagcg tgctcgaaac
    17521 ccggcatatt tgccatcaat gtagttgatg tcaaaattcc tcgctgataa gcatccataa
    17581 tcccataatt cacaccatgg ctatagccaa aatcatccga attaaaaatc acttttccca
    17641 tttcattctc cttctttttt ctactaaaat aggcgcaacc aaata
//
LOCUS       DADXTH010000061        17662 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.061, whole genome shotgun sequence.
ACCESSION   DADXTH010000061 DADXTH010000000
VERSION     DADXTH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.061"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IWI97_002006"
     CDS             287..694
                     /locus_tag="IWI97_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463500.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IWI97_002007"
     CDS             708..1109
                     /locus_tag="IWI97_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463501.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IWI97_002008"
     CDS             1111..1482
                     /locus_tag="IWI97_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463502.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IWI97_002009"
     CDS             complement(1518..1820)
                     /locus_tag="IWI97_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463503.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2069..2269)
                     /locus_tag="IWI97_002010"
     CDS             complement(2069..2269)
                     /locus_tag="IWI97_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2463504.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2574..3806)
                     /locus_tag="IWI97_002011"
     CDS             complement(2574..3806)
                     /locus_tag="IWI97_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBC2463505.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4060..4629
                     /locus_tag="IWI97_002012"
     CDS             4060..4629
                     /locus_tag="IWI97_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2463506.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4807..5247)
                     /locus_tag="IWI97_002013"
     CDS             complement(4807..5247)
                     /locus_tag="IWI97_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBC2463507.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5405..6169
                     /gene="map"
                     /locus_tag="IWI97_002014"
     CDS             5405..6169
                     /gene="map"
                     /locus_tag="IWI97_002014"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBC2463508.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6201..7124
                     /locus_tag="IWI97_002015"
     CDS             6201..7124
                     /locus_tag="IWI97_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBC2463509.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7200..8339
                     /locus_tag="IWI97_002016"
     CDS             7200..8339
                     /locus_tag="IWI97_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBC2463510.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8332..9132
                     /locus_tag="IWI97_002017"
     CDS             8332..9132
                     /locus_tag="IWI97_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2463511.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9132..9959
                     /locus_tag="IWI97_002018"
     CDS             9132..9959
                     /locus_tag="IWI97_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2463512.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9937..10671
                     /locus_tag="IWI97_002019"
     CDS             9937..10671
                     /locus_tag="IWI97_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2463513.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10771..11637
                     /gene="rfbA"
                     /locus_tag="IWI97_002020"
     CDS             10771..11637
                     /gene="rfbA"
                     /locus_tag="IWI97_002020"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBC2463514.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11651..12223
                     /gene="rfbC"
                     /locus_tag="IWI97_002021"
     CDS             11651..12223
                     /gene="rfbC"
                     /locus_tag="IWI97_002021"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBC2463515.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12245..13273
                     /gene="rfbB"
                     /locus_tag="IWI97_002022"
     CDS             12245..13273
                     /gene="rfbB"
                     /locus_tag="IWI97_002022"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBC2463516.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13371..14222
                     /gene="rfbD"
                     /locus_tag="IWI97_002023"
     CDS             13371..14222
                     /gene="rfbD"
                     /locus_tag="IWI97_002023"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBC2463517.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14257..16290
                     /locus_tag="IWI97_002024"
     CDS             14257..16290
                     /locus_tag="IWI97_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463518.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16334..17614
                     /locus_tag="IWI97_002025"
     CDS             16334..17614
                     /locus_tag="IWI97_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBC2463519.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         5534 a         2975 c         3599 g         5554 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     2041 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     2101 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     2161 tagatgcatg cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat
     2221 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga
     2281 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccaacgaaag atatatcgtt
     2341 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2401 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2461 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2521 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2581 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2641 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2701 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2761 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2821 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2881 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact
     2941 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     3001 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     3061 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     3121 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca
     3181 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3241 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3301 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3361 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3421 agtgctttgc cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat
     3481 gcatcgggat cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc
     3541 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
     3601 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
     3661 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
     3721 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
     3781 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
     3841 ctattagcat gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga
     3901 ggttattttt ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg
     3961 aagaaaatca aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat
     4021 ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt
     4081 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     4141 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     4201 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     4261 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     4321 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
     4381 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     4441 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     4501 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     4561 attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa
     4621 atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat
     4681 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     4741 gaatgcaatg aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca
     4801 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     4861 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     4921 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     4981 tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc
     5041 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     5101 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     5161 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     5221 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     5281 tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg
     5341 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     5401 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     5461 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     5521 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     5581 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     5641 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     5701 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     5761 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     5821 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     5881 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     5941 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
     6001 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
     6061 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
     6121 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
     6181 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
     6241 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
     6301 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
     6361 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
     6421 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
     6481 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
     6541 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
     6601 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
     6661 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
     6721 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
     6781 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
     6841 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
     6901 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
     6961 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
     7021 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
     7081 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
     7141 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
     7201 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
     7261 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
     7321 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
     7381 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
     7441 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
     7501 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
     7561 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
     7621 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
     7681 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
     7741 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
     7801 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
     7861 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
     7921 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
     7981 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
     8041 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
     8101 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
     8161 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
     8221 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     8281 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     8341 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     8401 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     8461 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     8521 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     8581 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     8641 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     8701 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     8761 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     8821 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     8881 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     8941 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     9001 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     9061 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     9121 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     9181 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     9241 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     9301 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     9361 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     9421 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     9481 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     9541 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     9601 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     9661 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     9721 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     9781 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     9841 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     9901 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     9961 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
    10021 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
    10081 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
    10141 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
    10201 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
    10261 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
    10321 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
    10381 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
    10441 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
    10501 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
    10561 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
    10621 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
    10681 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
    10741 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
    10801 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
    10861 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
    10921 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
    10981 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
    11041 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
    11101 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
    11161 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
    11221 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
    11281 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
    11341 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
    11401 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
    11461 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
    11521 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
    11581 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
    11641 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
    11701 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
    11761 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    11821 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    11881 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    11941 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    12001 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    12061 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    12121 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    12181 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    12241 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    12301 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    12361 aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    12421 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    12481 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    12541 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    12601 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    12661 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    12721 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    12781 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    12841 tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    12901 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    12961 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    13021 cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa
    13081 cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct
    13141 aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    13201 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    13261 aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag
    13321 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat
    13381 taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc
    13441 tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg
    13501 cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag
    13561 ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa
    13621 atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg
    13681 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    13741 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    13801 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    13861 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    13921 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    13981 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa
    14041 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    14101 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca
    14161 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    14221 aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca
    14281 cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt
    14341 ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta
    14401 aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa
    14461 actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca
    14521 cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta
    14581 aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga
    14641 aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg
    14701 gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag
    14761 aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag
    14821 tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct
    14881 ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg
    14941 gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg
    15001 gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca
    15061 tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg
    15121 gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca
    15181 ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca
    15241 aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc
    15301 tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat
    15361 agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg
    15421 attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca
    15481 ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct
    15541 tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat
    15601 gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt
    15661 gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg
    15721 agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat
    15781 ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa
    15841 atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc
    15901 ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg
    15961 aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga
    16021 gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc
    16081 acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca
    16141 ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa
    16201 gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa
    16261 actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt
    16321 agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag
    16381 tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg
    16441 ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc
    16501 tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt
    16561 tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt
    16621 tggagactta ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg
    16681 gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt
    16741 atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa
    16801 ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc
    16861 tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg
    16921 gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt
    16981 cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg
    17041 tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc
    17101 ggctgtctct gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta
    17161 tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca
    17221 gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga atatctatgg
    17281 attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt
    17341 gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat
    17401 gcagtcattt atggaggata cattgacttc ataccgatgg gttccgatta tttatatcct
    17461 gttggctatg gccgttcatc gatatgatga accagaagtt acggaggaat tctatgttat
    17521 ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag
    17581 cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt
    17641 gacaaccgtc gcagcctttt tt
//
LOCUS       DADXTH010000062        17448 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.062, whole genome shotgun sequence.
ACCESSION   DADXTH010000062 DADXTH010000000
VERSION     DADXTH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.062"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            224..592
                     /locus_tag="IWI97_002026"
     CDS             224..592
                     /locus_tag="IWI97_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBC2463520.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            772..1713
                     /locus_tag="IWI97_002027"
     CDS             772..1713
                     /locus_tag="IWI97_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBC2463521.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1782..2513
                     /locus_tag="IWI97_002028"
     CDS             1782..2513
                     /locus_tag="IWI97_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBC2463522.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2657..4837
                     /locus_tag="IWI97_002029"
     CDS             2657..4837
                     /locus_tag="IWI97_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBC2463523.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4857..5339
                     /locus_tag="IWI97_002030"
     CDS             4857..5339
                     /locus_tag="IWI97_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBC2463524.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5355..6116
                     /locus_tag="IWI97_002031"
     CDS             5355..6116
                     /locus_tag="IWI97_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBC2463525.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6247..7581
                     /locus_tag="IWI97_002032"
     CDS             6247..7581
                     /locus_tag="IWI97_002032"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBC2463526.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7888..8373
                     /locus_tag="IWI97_002033"
     CDS             7888..8373
                     /locus_tag="IWI97_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBC2463527.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8366..9049
                     /locus_tag="IWI97_002034"
     CDS             8366..9049
                     /locus_tag="IWI97_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBC2463528.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9175..11820
                     /gene="polA"
                     /locus_tag="IWI97_002035"
     CDS             9175..11820
                     /gene="polA"
                     /locus_tag="IWI97_002035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBC2463529.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11865..12701
                     /gene="mutM"
                     /locus_tag="IWI97_002036"
     CDS             11865..12701
                     /gene="mutM"
                     /locus_tag="IWI97_002036"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBC2463530.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12665..13294
                     /locus_tag="IWI97_002037"
     CDS             12665..13294
                     /locus_tag="IWI97_002037"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBC2463531.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13346..13678
                     /locus_tag="IWI97_002038"
     CDS             13346..13678
                     /locus_tag="IWI97_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBC2463532.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13990..14481
                     /gene="nrdR"
                     /locus_tag="IWI97_002039"
     CDS             13990..14481
                     /gene="nrdR"
                     /locus_tag="IWI97_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBC2463533.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14504..15877
                     /locus_tag="IWI97_002040"
     CDS             14504..15877
                     /locus_tag="IWI97_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBC2463534.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15877..16803
                     /gene="dnaI"
                     /locus_tag="IWI97_002041"
     CDS             15877..16803
                     /gene="dnaI"
                     /locus_tag="IWI97_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBC2463535.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16932..17372
                     /locus_tag="IWI97_002042"
     CDS             16932..17372
                     /locus_tag="IWI97_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBC2463536.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5955 a         3042 c         3831 g         4620 t
ORIGIN      
        1 gtttttagtc acgacctctt tttttattta ttccatataa atacacatta tttattccga
       61 aaagtgaaag cgcaaataac aaaaacaacc aacttattta ttattaaaga aaagcgtatt
      121 taagtaggac gattctgttg agagctttcg tagattttcc cattttccta tggtaaactg
      181 acaaagttga gaatgttaga acagatcgtt taggaggaaa cagatgttta cgaatgaaat
      241 aaaaatcatg ctttatgtgg acgatgtaga aaaaaatgca acgttttgga gagcagttgg
      301 ctttgcggaa aaagatcgtc aagaaatgga tgggacgctt ataattgaag taggagttga
      361 ggacagtcaa acctcatttc tgttatatga tcgagcattt gttgagtccc attcacctgg
      421 cacagctact agtgccccat ctttgatgtt ttacagtaaa gatatctttg gactatataa
      481 aaaaatgcag acgcaatcag tcacattagg tgatatggta cagcttggag aagaatatgt
      541 ctttaacttt gctgatcccg atggcaacta ctttgcggtt acaggtaaat aatagaaata
      601 aagactttgt ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata
      661 ttatgtaaac aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc
      721 gaacgccttc tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat
      781 caagtactgc tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat
      841 catttagcct tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga
      901 ataaatggta ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa
      961 gctgatcctc gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt
     1021 aagaagatgt ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt
     1081 gatccaaacc aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta
     1141 gatgaacaga cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt
     1201 cgaggggctg tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag
     1261 cacaaagaag aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc
     1321 gagaagtttt ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc
     1381 ggtattgcta cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg
     1441 tatgtgtttg atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt
     1501 cgcgactggt ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc
     1561 aatcgtcaga ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata
     1621 gagtgtgcga aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa
     1681 gcagaactgc ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat
     1741 ttccaatatg cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg
     1801 ctattgggga tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc
     1861 caaagatcca tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag
     1921 aatgtttttt attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa
     1981 accatgaaga atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata
     2041 tgagaaatgg tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat
     2101 actccccaac tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt
     2161 tagtccagcg ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg
     2221 atttgacaaa gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac
     2281 catttcatga agggaaaaac aatacaggta agacaattgt atttggacat acgattacac
     2341 caatgctcca tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa
     2401 tggacggtgg ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta
     2461 ttcttcaaga tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg
     2521 aaagaatgaa ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt
     2581 tctttcagta gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca
     2641 gaaaagaggc attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa
     2701 agaactttca gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg
     2761 aaacactgtt ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt
     2821 ccagattcgt gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac
     2881 tgaagtgatt cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat
     2941 cacccaagcc gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg
     3001 acggacaaaa gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat
     3061 gcagcttaca gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga
     3121 agtctcttct gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag
     3181 cgataatgca aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt
     3241 cagcaatgtc aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt
     3301 tgcagaaccc gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa
     3361 agaagatatt ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga
     3421 tcgccaaata gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca
     3481 ggatagttat aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac
     3541 tgaaaaggca gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt
     3601 gcaaccgcca ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg
     3661 taaactagca gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca
     3721 taaacctgca gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa
     3781 taaatatcaa gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact
     3841 ttttgtggct gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga
     3901 agcaggcgct tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca
     3961 agttgaagaa agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt
     4021 agtcaaaatc gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa
     4081 acgtttggct gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga
     4141 tgtgaataca gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc
     4201 ccaaaacatt gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa
     4261 aaaagtgcca cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc
     4321 tggtgggaaa aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa
     4381 agaaatatta acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga
     4441 aaaattgaca cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac
     4501 gttagccgat attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc
     4561 agcgccctta ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt
     4621 gactggaacg gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca
     4681 agacggactt gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt
     4741 cgtgtcagta ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggtag
     4801 aatcagtttg acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga
     4861 cacaagaaga actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt
     4921 tcaagcacca agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa
     4981 acgatcatca tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg
     5041 caggaatcat caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc
     5101 agcataaaga tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac
     5161 cagccttgac gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca
     5221 cgatctgccg caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag
     5281 aaatcaaatg gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa
     5341 aggatagaag gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat
     5401 taaatttttt agccgcacct gaagagggaa attttgagga actgacggca cctacactta
     5461 ttttagctgg aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg
     5521 gccaagtaga tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg
     5581 agaattttga aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc
     5641 gtcagtatct aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt
     5701 ctacaaattc tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg
     5761 taccagaaaa aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt
     5821 ttgagaaagt atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg
     5881 aaatccttca cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc
     5941 ctaaggaata tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga
     6001 acggctatgg tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt
     6061 ggtcttctta caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat
     6121 tttagcaagc gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag
     6181 ataatgagga gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg
     6241 attcaaatgg aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg
     6301 agctccttgg ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa
     6361 aaatatttct ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac
     6421 gcagataatg taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat
     6481 gaagaaatcc agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt
     6541 ggtcatttta tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt
     6601 acgactggac tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga
     6661 gatggaacag gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat
     6721 cgccgtcatt tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat
     6781 catccggatt attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga
     6841 caagtgaaaa aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca
     6901 aatgtgccga tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc
     6961 gaacgcacaa cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac
     7021 ttcactgtac cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta
     7081 gcttatttcg aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga
     7141 gtaaaacgtc gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg
     7201 catcaccctg cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa
     7261 gaaataattg cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa
     7321 tttgcagaag cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct
     7381 cgggaagaac aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga
     7441 gaagtcatca aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc
     7501 ttcatgggtg ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc
     7561 acgacgaaaa atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg
     7621 ggagtggcaa aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa
     7681 agcttgcatc tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt
     7741 ggctgttctt attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat
     7801 ctgttaaatc aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga
     7861 ttaaaaataa actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa
     7921 acaataaatg aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac
     7981 cagcttttgc tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac
     8041 tttgcacaaa aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc
     8101 atcacaagtg caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct
     8161 gtcaatttta ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata
     8221 aaagtagata agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa
     8281 gatcgtgtgt tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata
     8341 gcaatcgaaa gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa
     8401 tcgtttttat gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac
     8461 ttcttacttg gctctttggg tatttccttt gcaattagca acttttatca ataattttcc
     8521 actaggcttt gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa
     8581 agcacaaaag aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg
     8641 cataacatta gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt
     8701 gacagctact ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct
     8761 tagcggggtt ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt
     8821 gaacatcttc attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt
     8881 catctttgca gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac
     8941 aaacggaaca gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt
     9001 tatcaacttg ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa
     9061 attcatcagt tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt
     9121 ccgcaggatt ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca
     9181 aaaaataaat tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt
     9241 cacaattctc ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca
     9301 ttcaatacga tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg
     9361 ttcgatgcag gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga
     9421 tccaaaacac ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga
     9481 ctaggggtac agtattacga attgcccaat tatgaagcag atgatattat cggtactttg
     9541 gcagaaaaag tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg
     9601 actcaattag ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc
     9661 gaaagctata caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt
     9721 attgacatga agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc
     9781 ggtgaaaaaa cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa
     9841 catatcgatg aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag
     9901 gctttcttat cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc
     9961 gactcattga agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg
    10021 gatttcaatc agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat
    10081 attttgtttg aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg
    10141 tacgtggaaa tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg
    10201 acagaaaaga aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat
    10261 tcttggatca ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt
    10321 gctttaaacc gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca
    10381 taccttctcg atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga
    10441 tacaacgata tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca
    10501 gaagaagagg aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg
    10561 acaccaaaat tgagccaaga actggcagaa aaaaaccaag aagatctttt ccggaaaatg
    10621 gaactgccat tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg
    10681 actcgcttac aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa
    10741 atctacgaag aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc
    10801 ttatttgaaa aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc
    10861 gtagatgtac tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat
    10921 cgtcaaattg caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca
    10981 gatggaaaag tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct
    11041 gttgatccta atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag
    11101 gcatttgttc ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag
    11161 ttacgcgtac ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt
    11221 caagatatcc atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc
    11281 acaccaaata tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct
    11341 gattacggac tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat
    11401 acgtactttg aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca
    11461 aaagaccaag gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat
    11521 tcaagaaatt acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa
    11581 ggaagtgctg ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa
    11641 acaggtcttc aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg
    11701 gaaaaagaac ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct
    11761 cttcatgtac cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa
    11821 acattgttta agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag
    11881 aagtagaaac cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag
    11941 ttcaggtctt gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc
    12001 ttgttggcga aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg
    12061 atcattgtga actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa
    12121 atacaccaat cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc
    12181 gctacaacga tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa
    12241 cttaccgagg aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag
    12301 atttcgcaaa tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac
    12361 gtctagtgac tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc
    12421 atccagaaca accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga
    12481 tcattgatgt tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga
    12541 atgcattagg agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca
    12601 aaccatgtat acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc
    12661 actattgtcc gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac
    12721 gggtggaata gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc
    12781 tttgatcgat ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag
    12841 gaaaattgta tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg
    12901 gaaacttggg atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt
    12961 agatccgttt atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc
    13021 tttagtaatt gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc
    13081 agtagctgtc gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca
    13141 actgacgcta gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa
    13201 gcagcgtgcg gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat
    13261 cgaaaattgg ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg
    13321 agtatataga aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt
    13381 aggtaacgca aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg
    13441 tcgtacgcaa tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga
    13501 aatagaagac cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa
    13561 aaatccgaca cctgatcgta gtaaaacacc gctcccagaa agtgaccaca ttcccgatga
    13621 gcaaaaaatc gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa
    13681 tagtaaaaaa tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaagttt
    13741 tttgtgctag atgactaaat cagcttttgt gtaagttatt tctcagtaat caaggtgatc
    13801 agtattggaa aattgaaatt tagtaaggac aggggacaca attacgcatg aaagtagaaa
    13861 aaattgttct tatatccgtg ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg
    13921 ttataataca actagttgta aagaaagaca catcctttat ataaaatgcg atgcaagagg
    13981 tgagacaaaa tgagatgtcc cagatgtcaa cataataatt cacgagtaat cgacagccgt
    14041 caagcagatg atggacgtgc gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga
    14101 ttcactacat ttgaacgaat tgaagctgct ccgttattag taatcaagaa aaacggggaa
    14161 cgcgaagagt tcaatcgcga taaaatttta agaggactca tccgttcagc agaaaaaaga
    14221 cctgtagcta tggaacaaat ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt
    14281 ctcggagaaa acgaagtatc gacgaatcta attggtgaat atgtgatgga agatttagtt
    14341 gatttagacg aaatcgctta tattcgtttt gcaagtgttt atcgccagtt caaagatatg
    14401 tctgttttct taaaagagtt gcaagatatc gtggataaag caaaagaaaa taataaagag
    14461 aataataaag aaaacaatta agaaacaaaa aggaggcatt tccttggaaa atgcttggaa
    14521 agagcttcaa ccaaaaaata tttaccaagg aagaaaagcg gctccgctga gagaagacag
    14581 caagaacgcc ttgctgtatc tttatcagcc actaatcggc ggagaagcct tgagtatcta
    14641 tttcaccctg ttatcagaaa tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc
    14701 agatctgctt agtagtctag gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct
    14761 agaaggaatc ggcttactgg atgtttattt taaagaagat agtgatctgg gacctcgttt
    14821 tatttatgag ctgttagaac cactcattcc taaagcattt ttcaaagact ctgttctggc
    14881 ttttcttctt ctggaaaaag ttggtgaacg acgattccag aagcttaccc aattctttga
    14941 acctaaaagg catacagcag agggatatca aaaaatcaca aaaaaatttt tagatgttta
    15001 tcgcttcaat gaagcagtgt acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc
    15061 ttttgaagaa tctgaatcag tagcattact tgaagcagat tcgacattcg attggcagtt
    15121 tttgaatgat tggctggcaa aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca
    15181 agtcgacatg tatcatcagc tttacggttt cgatgaattg acacttggcg aactgatcag
    15241 tcaatcttat gactttagtg aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt
    15301 ggcaatgcag agacgtccgg tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag
    15361 taatgaaccc tcgtcaaacg aacatttgac aaacgcagcg cttcagctga taaaagaagc
    15421 acagagcatt ccaccgatga aatatctgga agcaatcaag tcagaaaaag gcggatatgt
    15481 ttctaaacaa gaaaactggt tactgcaaga tcttgtttct caatcaggtc tttccagtag
    15541 cgtgatcaat gttttgctga attatgtact agtgatcaag aataatgcta gcttaaatgc
    15601 cagttatgta aatacagtgg caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga
    15661 agcgatccag cacattcggc aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca
    15721 aacaaattat caaactggca agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa
    15781 ccagccaaaa gatgaaaaac agatcagcag cgagaaaaaa gcagagattg accgccgttt
    15841 taaagaatac ctggcaaaaa aggagggtga tatctagtgg aagacgtagg caaagaattg
    15901 aagaggatca tcagcaaacg caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc
    15961 ttaaatgatg cggatgttca agcttttcta agcgcacacc aagaagaatt gacacaagcg
    16021 gatattgaaa aaagttacgc taaattgtat gaattcgtgc aagaaaagcg taaatatcaa
    16081 aaaaacgatc caacgatgat cgctcccggc tatgagccga agctgacgtt gaattttcat
    16141 tatgtggatg tcacttatgt accaacggaa gcattgttag cacggcaaag acaagaagaa
    16201 atcagaagcc gagtaaaagc aatggatatg ccaaaagata ttcaatcggc ttcttttgaa
    16261 aactttgaac gtacagatgg acgaggatat gcgagcttag aagccctaga ttttgtagag
    16321 aaatatactg ctgatccaaa agggtatcat aaaggactgt atctagtagg aagttttggt
    16381 attggaaaaa catatttatt aggagcaaca gcgagagaat tagcgattgc tggattcacc
    16441 acgacgctag tccattttcc gacatttgcc gttgagatga aacaggcaat cggaaaagat
    16501 caagtggcag aaaagttaga tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt
    16561 ggtgcagatg cgatgagtag ttggatacga gacgaagtat tcggtgttat ccttcaatat
    16621 cggatgcagg aacagttacc aaccttcttc tcatcgaatt ttacaatgga tgaattggaa
    16681 cagcatttgt ccgtcactca acgtggagat gaagagccgc taaaagcgaa acggataatg
    16741 gaacgcatac gttatttgac aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt
    16801 tgaacagttg acaagtccaa ctgacatttg acctagtagt aaaagcaggt cagcgaatca
    16861 ttatatggac agaaatgacc ggagacaatc accgcaacaa gtaatgcaga aacttaaacg
    16921 gaggattttt tatgagggct attttttcta gtatatctgt agttggtcta ttttttcttt
    16981 tagcaggctg cggtagcgca gatagcaaca caggttctgt tgattcgatt gatgcgggtc
    17041 aaacagtaaa aacaagcagt acgaaaacgg agagacaaag catatttgtc ggaacgattg
    17101 aaagtatcac tcaagtagaa gcagaagaag aaacgcttca gttgaaactc gtcgatgtca
    17161 tggccaaaga agatccagcg aatatgggaa cgtcattcag caatgacgga gtgatcttaa
    17221 atgctactgc tgaaaaaata agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc
    17281 aatttacatt agttgagatg ccgatcatga cgatgtctat cccaccgcaa gttcctggag
    17341 attcgattat tgatgtttca ttgaacaaat aaccaataac attttttcaa aaataagaaa
    17401 atgttgtttt atagaacgaa aaggtatatt ttttaaggag atatggga
//
LOCUS       DADXTH010000063        17148 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.063, whole genome shotgun sequence.
ACCESSION   DADXTH010000063 DADXTH010000000
VERSION     DADXTH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.063"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(44..2494)
                     /gene="parC"
                     /locus_tag="IWI97_002043"
     CDS             complement(44..2494)
                     /gene="parC"
                     /locus_tag="IWI97_002043"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBC2463537.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(2506..4551)
                     /gene="parE"
                     /locus_tag="IWI97_002044"
     CDS             complement(2506..4551)
                     /gene="parE"
                     /locus_tag="IWI97_002044"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBC2463538.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(4717..5151)
                     /locus_tag="IWI97_002045"
     CDS             complement(4717..5151)
                     /locus_tag="IWI97_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBC2463539.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5278..5916
                     /gene="plsY"
                     /locus_tag="IWI97_002046"
     CDS             5278..5916
                     /gene="plsY"
                     /locus_tag="IWI97_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBC2463540.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(6048..6923)
                     /locus_tag="IWI97_002047"
     CDS             complement(6048..6923)
                     /locus_tag="IWI97_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBC2463541.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(7005..7796)
                     /gene="codY"
                     /locus_tag="IWI97_002048"
     CDS             complement(7005..7796)
                     /gene="codY"
                     /locus_tag="IWI97_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBC2463542.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(7814..9214)
                     /gene="hslU"
                     /locus_tag="IWI97_002049"
     CDS             complement(7814..9214)
                     /gene="hslU"
                     /locus_tag="IWI97_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBC2463543.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(9228..9776)
                     /gene="hslV"
                     /locus_tag="IWI97_002050"
     CDS             complement(9228..9776)
                     /gene="hslV"
                     /locus_tag="IWI97_002050"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBC2463544.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(9813..10718)
                     /gene="xerC"
                     /locus_tag="IWI97_002051"
     CDS             complement(9813..10718)
                     /gene="xerC"
                     /locus_tag="IWI97_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBC2463545.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(10916..12292)
                     /gene="trmFO"
                     /locus_tag="IWI97_002052"
     CDS             complement(10916..12292)
                     /gene="trmFO"
                     /locus_tag="IWI97_002052"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBC2463546.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(12261..14339)
                     /gene="topA"
                     /locus_tag="IWI97_002053"
     CDS             complement(12261..14339)
                     /gene="topA"
                     /locus_tag="IWI97_002053"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBC2463547.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(14461..15318)
                     /gene="dprA"
                     /locus_tag="IWI97_002054"
     CDS             complement(14461..15318)
                     /gene="dprA"
                     /locus_tag="IWI97_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBC2463548.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(15374..16141)
                     /locus_tag="IWI97_002055"
     CDS             complement(15374..16141)
                     /locus_tag="IWI97_002055"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBC2463549.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDVRSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(16134..16994)
                     /gene="ylqF"
                     /locus_tag="IWI97_002056"
     CDS             complement(16134..16994)
                     /gene="ylqF"
                     /locus_tag="IWI97_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBC2463550.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         4376 a         3825 c         2976 g         5971 t
ORIGIN      
        1 gtattaatac agatggcgtc tcttcattaa aatactacaa cagttacgac tcgatgaaaa
       61 tctcaggagc atcgataact tcgatgactt ctccaccttt tttctcatcc atcgcaaatg
      121 atccattcga tgttcgatca cctataggga aatcagttgg gtcaatcgtt aactgctgcc
      181 ctttttgatt catcaaggtt atttcttttg atcttccttc cgacatatag ataatacggt
      241 gcggattttt tttcagctca cgcatgaccg tcactccccg cttcgcacgt gaagtttggc
      301 tgatttcttg agcaagcatg cgtttaacag ctcctcgctg agtaacgatt acgatcggcg
      361 tatcaccttc tgctaacaca agcagcccgt tgataatcct atctccatct ttcaaattca
      421 tagacttcac tccagctgcc tttgctccaa caacgggaac atctgttaaa ggatagcgca
      481 aacccattcc ttgatgcgtt actaagaaaa catctaagcc tggctgatct tctttcagat
      541 acacagcaat cagttcatct tcttccgcct ttagcttcat acagctgagc ggacggctct
      601 tataggtgcg ccatggttcg aactctgtca ttcgtgtttg cttgatcaat ccattcttgc
      661 tgatgaatac aaatgttttc tctgcttgaa ttttttgata tgggaaaaca gctaaaatcg
      721 attcgtctac agacagattg gagattgttt gagaaatgtg ctcaccagca tctttccatt
      781 tcaaatcggg tagttcatgg acagggcggt agatcacatt tgctttattt gtgatcagaa
      841 gaacatggtc caatgtactc aattcacctg agtatagcaa gtagtcgcct tctcgcatcc
      901 cgatttcttc tggtttggaa gcactataag aacgaaggct tgttcgcttc acatagcctt
      961 ctcgcgtcac actgacaatt acgtcttctt gtgccactaa gacttgcgta tcaattttga
     1021 tttcttcgat ttcgtcttcg attgtcgtta gacgagcgga agaataattt tttttcactt
     1081 ctcgcaattc ttttttcata acagagaaaa gctctttgtc atttgaaagg attttattca
     1141 actctgtgat ttgcgcaatc aagctttcgg attccttttg aagctctgtg atatccgtgt
     1201 tcgtcaaacg gtataattgc aatgtgacaa ttgcttctgc ttgttcttct gtaaattgaa
     1261 agacgtcgac taaattcttt ttggcgtctt ttttgtcttt actttcacga atcgttgcga
     1321 ttacttcatc cagaatcgat aaagctttca tcaatccttc tacgatatgc tgtctttttt
     1381 gagctttggc taaatcgaat tgactacgtt ttgttatgac ttgctttcga tgctcgatat
     1441 aacttcttaa aatgtctttc agtccaactt gatgcggagt catatggtcg atagcgacca
     1501 tattgaaatt gtaattgatc tgtagctcag tgtttttgaa caaataattc aaaattcctt
     1561 gagcattggc gtcttttttc aattcgacga caatttgcag acctgttcta tcactttcat
     1621 ctcgtacttc agctattcca tcgattttct tattcaaacg gatctcatcc atttttttca
     1681 ccaaggtcgc tttatttact tcatatggga tttctgtgat cacgatttgc tgtttattac
     1741 ccttcattgc ctctatcttt gttttcgaac gaagaataac ttttcctcgt cctgtttcat
     1801 aagctttttt gatttcatct tttccttgca agatccctcc tgttggaaag tcaggtccag
     1861 gaatatattc catcaatttt tcaaggctcg cttgtggatg atcgatcata tagattgtgc
     1921 catcgatcac ttcagctaag ttatgagtcg ggatttctgt tgcatatcca gcagatatac
     1981 ctgtcgatcc attgacgaga aggtttgggt atcgagctgg aaggactgtt ggttcttttt
     2041 ctgtatcatc aaaattccac acgagatcga ctgtttcttt ctcgatatct gctagcatct
     2101 ctccacttaa cttggataat ctggcttcag tataacgcat cgcagcaggt ggatcaccgt
     2161 ccatactccc gttattcccg tgcatttcga tcagtacttc ccgcaacttc cagtcttggc
     2221 tcatgcggac catcgcttca taaatactga tgtcaccatg aggatgataa ttccccatga
     2281 tatttccgac agattttgct gatttacgga atcctttgtc atacgtattg ccatctttgt
     2341 tcatggcaaa taatatccgg cgttgcactg gcttcaatcc atcacggata tcaggcagtg
     2401 cacgttcttg gataatatat ttggaataac gtccgaagcg gtcacccatc acttcttcta
     2461 aagttaattc ttgtacttct tgtcgatttt ccatcaggtg acctcctatt ctacttcaaa
     2521 taactgattg ttttcatttt tatctgctcg ttcttcatct aataaatcat tagaaactga
     2581 aggagagatt tcagtatcct cttttttatc taagatactg ccatcttctt cgagcgtaaa
     2641 ttgcacgtga ttttcgatcc attttcttcg aggttctact ttgtcaccca tcagtgtggt
     2701 tactcgacgt tcagcttgcg cagcatcatc gatacggacg cggatcaacg tacgggaagt
     2761 cggatccatc gttgtttccc acaattgttc cgcattcatc tctccaagtc ctttatatcg
     2821 ctgcagcata tatcctttgc cgactttttt aatcattgct gccaattcgt catctgtcca
     2881 cgcatattca atgacttgct tctttccttg tcctttggat actttatata aaggcggtaa
     2941 cgcgatatat actttcccag cttcaatcag cggtttcata taacgataaa agaaagtaag
     3001 cagtaatact tgaatatggg cgccgtctgt atccgcatcg gtcatgatga ttactttgtc
     3061 gtaattacag tcttcgatag aaaactcagg accgactcct gcaccaatcg tatagatcat
     3121 cgtattgatt tcttcatttt tcaagatatc ttgcatttta gctttttcgg tattgatgac
     3181 ttttcctcgt aaaggtagaa ttgcttggaa ctttctgtct cttccttgtt tagcagatcc
     3241 gccagcagaa tccccttcga ctaaatacaa ttcatttttc ttagggtttc gggattgtgc
     3301 gggtgtcaat tttcctgaaa gaagagattc gcctttttta cgcttcttcc catttcggct
     3361 ttcttctctg gctttacgtg ctgcttctct cgcttctctg gctttgattg ctttacggac
     3421 caacatctgg ctcatttcgc tattttcctg caaataaaag cccatttgtt cactgatgac
     3481 gttatctact acggtacgag caactggcgt accaagtttt tcttttgttt gcccttcaaa
     3541 ttgcagaaga ttttcaggta cacggataga aagtacagca gctaaccctt ctctgaaatc
     3601 actaccttcc agattcttgt cccgttcttt cagcaaacca acttttctcg catattcatt
     3661 ataagatttc gtcatcgccg ctttcatacc agcttcatgg gttcccccat cttttgtccg
     3721 aacgttattg acaaaagata aaacattttc tgaataaccg tcattgtatt gataagcgac
     3781 ctctacttcg ataccttctt tttctcctga aaaataaaca actggtgtca atgtatcttt
     3841 ttcttcattc agataatcaa caaattcttt gattccttct tcgtaatgaa aaatttcctt
     3901 gactggttct tctccacgaa gatcagaaag agagatttta acgcctttta gaagaaaggc
     3961 agattctctt aaccgttcgg ctaaaatttc atatgaaaat ttcgttgtac tgaatattgt
     4021 atcatctggt aaaaaatgga cagtcgtccc gtttgctttt tttgtttttc ctatcttttt
     4081 caaagtaccg tcaggtttac cgccgttttt aaatttctgc tgatactcta cgccatctct
     4141 aacgatcgtt acaattagcc atttcgataa ggcatttacc acactggctc ctacaccgtg
     4201 cagtccacca gaagttttgt accctccttg accaaacttt ccgccagcat gcagcacagt
     4261 aaaaatcact tcaactgttg gtattccgga agcatgcatt cctacaggca tcccacgtcc
     4321 gctatctgca acagttatac tattgtcttc atggatggtc acatcaatct cactgccata
     4381 acctgataat gcttcgtcaa ctgcattatc gacgatttca tagactaaat gatgcaagcc
     4441 tcggctgtca gtggatccaa tgtacatccc tggtcttttt ctaactgcct ctaatccttc
     4501 caatacttgg atggacgagt cattgtattc gttgtttact ttttttgcca aggcaaacgc
     4561 tccttcaata atactttgcg gcaaaaccgc accacgatta tcatactaga aaaggtcgtg
     4621 aaaaaaaaga gggtagacga gaaatcaatt ttaattatct cctaaccgct ttttcgacat
     4681 tcccctctat cgatcatctc ttctttgtgg tcatttttat ttttggtttt ctttcaactt
     4741 accaagttca atcttaatac aacgatccat tacgatatct gtacgtcctg cttttcttag
     4801 catctcagct gcttctttgt tttcaatccc taattgtgcc cagaacactt tagcatcagt
     4861 tttcaaaaaa tcacctgcta tttcaggtaa aaattcactc cgtctaaata catctacgat
     4921 atcgattgga tcatcgattt ctgtcaaatt cgcatagact ttctcgccta gaatttcttc
     4981 accagccaat aatggattta ctggtatgat tttatatcca ttgtcttgca atacctgtgc
     5041 aaccatgtaa cttgttcgtt ctggtttatt gctcaagcct acgacggcaa tattttttgc
     5101 ttcttccaaa tagttgaaaa tcgtttcttg tgttggattt tttacagaca tttctgccac
     5161 cccttgcttt tctttttatt ccatctatta tacttgtatc tttcattttt tcaattgttc
     5221 ctgttaccta ttcatgctaa aataaaggat agttttcaac ttgaaagagg gtaaaaaatg
     5281 aaattgatct tattacttat tttagcatat cttttaggtt ctattccttc cggtgtctgg
     5341 attggcaaat tgtttttcaa aaaagatatc cgacaacacg ggagcggtaa tactggaacg
     5401 accaatacat ttcgtgtatt aggcaaaaca gccgggattg ttgtcctgct tatggatatt
     5461 ttaaaaggta cattggctac ttgtttgccg atgatcttcc acctttctgg gattaaccct
     5521 ctttggtttg gggtatgcgc cattcttggc catacatttc cgatttttgc tgggttcaaa
     5581 ggaggaaaag ctgttgcgac aagtgcagga atgcttttag gatttaatcc tgtatttttt
     5641 gtttattctt gtatcatctt tatcgttagt ttattttgta caagtatggt gagtttgagc
     5701 agtatgatca gtgcagtatt gatcacttta tctacgatcg tccttcctta tgtagctcct
     5761 gtgattttgg ctaagcccaa ttggttactg acgatcattg cctttttagt ttcttccttt
     5821 atcttttatc ggcataaaga caatatcaag cgtatcagaa accatactga aagtcgaatc
     5881 ccgtttggtt tgaatagcag caagaaaaaa tcctgatact ttttcattcc aaagagataa
     5941 aacgggagtg tgacaaatcg atgtcacact cccttattac ttacaggata gcttttttac
     6001 acaccttggt aaacggtgtt ccctgctaac ttctcggtca caacctctta cttcactgtg
     6061 atggaatatt ttgtcttgaa gatttcatgg gcacctagtt gattgatccc tactttttcc
     6121 atcaagtttc cactactgtt caccgtgtca gcgattccgc accacggttc gatacaaacg
     6181 aaaggcgctt cttttggata aggagaccag atccctacat aaggcatatc tttatagctt
     6241 aaagttacgc tatgggaaga tttttcactt cgaatggcgt acgcatttag accccttgtt
     6301 tcaaaaacca aggcatcttg ctcaaaaagt tcacgattca aagccagatt cgtatttgtc
     6361 tgacctaacg tcttttgatc catatcgatg aatgggcctt tcaatggtag gcgtaatctt
     6421 gatttcaatg gagaaaatgc cagatagaaa tcttcaaatg acaatccttc ttcaagaggt
     6481 acattgaaag caggatgtcc accgatagaa aagaacattt ctttttcacc ggtatttgcc
     6541 acttgataat gaaccgtgat gccgtctcct cctaattcat agctgatcac taattcaaaa
     6601 tcaaatggat acttttctaa tgtctccggc gtactcctta agatgaaact agccatttct
     6661 tgtccatgtt caatgacttc aaaatccata tcccgagcaa atccgtgttg ccccatttga
     6721 tatgttttcc cttgataaac atactgatca tcttttaaac gtccaacaaa agggaataaa
     6781 accggtgcat gtctgcccca atacgctgga tcaccttgcc agatatactc taaaccatgt
     6841 tcttttgatt tcagactgat caattccgca ccgtgttcag cgatttcggc gatcagctgg
     6901 tcattttgaa taataacagt cattgccatt cctccaactt cgacttactt tttaaaatgg
     6961 gaaggctgga acaaacattt ctatctgccc cagtcccctt acttctaata actgtgtttt
     7021 gctaactcgt ctttaaaacg ggaattcaat acttttaaat aagttccctt catgcctaac
     7081 gaacgagatt caatgattcc agctgattcc aattttcgta aagcattgac gatcacagaa
     7141 cgagtgatgc cgatactatc ggcaatatta gaagcagtca gacgaccttc atctccatcc
     7201 aatgcttcaa agatagcttg tacagccttc aattcgctat aagataatgt gttgattgcc
     7261 atttggacag ccgttgcact ccgtacatct gcttcgatgc ttcgagactt ctgataaagg
     7321 atctgcatac cgacaaccgt agctccatat tctgctaaga ctagatcgtc atctgcaaac
     7381 gattgttcca tacgagataa gataatcgta cccaaacgtt cgccagctcc aaagatcgga
     7441 acgacagtcg tcagaccaaa tggatatttc tctcttgact ctaccggaaa tgcagtaata
     7501 tcactagtaa ttgggatatt agcttcagtt ttcatcaact gtgccactgt atcagtataa
     7561 gattcaggaa attgtttctc aacaaacatt gttttgattc gttcattgtt cacatcgtga
     7621 cgttcattat aacctagtaa tactccgtca cttcctatga tataggtgtt gctgtcaaga
     7681 ataccaccta gtgtgacagc cattttatcg taaggcaaat ctgcttgcat gtcaaaagta
     7741 ttcttttgct gcaaaagtgt gtttatcttg cgggttttcg ttagcaagtc tgtcattgtc
     7801 gtcctccgat tttttataaa atatagcgac ttaaatcttc atttgccgca attgagccaa
     7861 gtttgtcatc cacatatgct tctgtgatcg ttatttctcc catttgcata tctggtgctt
     7921 caaataatag atcttctagt agtttttcca aaatggtatg cagtctgcgt gcgccgatat
     7981 tatctgtgtc acggttgaca tcgaaagcga tttgagcgat ccgttcaatt gcttcttttg
     8041 taaacgtaac tgacacattt tctgtaccga tcaacgcgac gtattgtttt actaaagcat
     8101 tatttggttc ggtcagaatc ttcacaaaat cttcagctgt caaatcatct aattctaccc
     8161 gtatcgggaa gcgtccttga agttctggaa ttagatcact tggtttagac aaatggaaag
     8221 ctcctgaagc aatgaatagg acgtgatctg tttggatgat cccgtatttt gtgtttactt
     8281 gtgaaccttc gacaattggc aatatgtctc tttggacgcc ttctctggat acttctccag
     8341 attgctgtga tttagaagtg atcttatcga tttcatcgat aaaaataatt ccactttctt
     8401 ccgctaattt gattgcctca ctatggatat cagcatcctt gactaatttt gctgattctt
     8461 ccttgatcaa taactctaaa gcttctttga ctgttaccgt ccgttccact tttttcttcg
     8521 gtgtcaaagc acctagagtt tcatttaggt cgatccccat ttgctccaat ccattgttca
     8581 tcattggtgc aggtttcttt tggtcttcaa cttcaatggt tacttcacga ctatccagta
     8641 aacccttttg tagttgttcc aagactgtac ggcgattgat tcgaatatct tctgtcactt
     8701 cttcttgcgg ttcagcggta ttttgctgac tagcattaaa aagattcatc atttgttcaa
     8761 attgattagt ggattgtttc tgttcttttt tgataccagg taccaagatt tttaccaaac
     8821 gtttttctgc atttttttta gcacttgtgt atacacggct gtactgctct ttttctacga
     8881 tttggatcgc attttctact aaatcccgaa ccattgattc tacatctctt ccgacataac
     8941 cgacttcagt aaattttgtt gcttcgactt tgacaaatgg tgcattgacg atttttgcca
     9001 aacgtcgtgc gatttctgtt ttaccgaccc ctgtaggtcc gatcatcagc atattttttg
     9061 gtgtaatatc ttgttgcatt ttttcatcta actgcaaacg gcgatagcgg ttacgcaaag
     9121 caacggcaac agattttttt gctgcgtgct gtcctacgat atattcatct aattcactta
     9181 cgatttgtct tggtgtttgt tttaattcat acatatctac tcacctctta cagttcttct
     9241 acgataatat tatgattagt aaagatacag atatctgcgg caatatttaa cgcattttct
     9301 gcaatttctt tcgcactcat gtcgttttga gcatagtttt tcattgctcg ggctgcagcc
     9361 aacgcaaaat tccctcctga tccgatagct aagataccat catctggtgt gataacttct
     9421 cctgtacctg aaacaagaag catttcttta tcattcatta cgatcagcat cgcttcaagt
     9481 ttttgcatag attgctgtgt ccgccattct tgagctaatt cgacagctgc gcgttgtaga
     9541 ttcccgttat attcgtttag tttgccttca aatttttctt ctaaagtaaa cgcatctgca
     9601 acacttccgg cgaaacctac aacaacttct ccattgtaga tacgtcgtac tttctttgca
     9661 gtgcctttca tgacaacaga ttcacccatt gttacttggc catctccagc catagcaaat
     9721 ttcccgtctt tttcgacggc acagatagtg gttgaatgaa atggtgattc gaccatgttt
     9781 gtttcctcct aaatttgttc ttttccttat ttttacgctc ttggatggaa agaacgatag
     9841 tttttttgta aactttcttt tgttacatgg gcatagatct gggtagtcga taaattggca
     9901 tgtcctagca attcttggac tgtccgcaaa tctgccccat tatttaataa atgagtagca
     9961 aatgtatggc gcagcatgtg aggatggata tcactatcca aactgctttt tttgatcatt
    10021 tgattcaaga catattcaat tccagtaggc gtgattggtt ttccatgatg attcacaaaa
    10081 acgatatcat gctcttcgtg atattttgtc atcaatacct ggcggccttc cctgaaatat
    10141 tcttgcagag catcttgagc aaaagaacct agaggagcat atctctcttt gttcccttta
    10201 ccatggatca acatgacctc tgaatcaaaa tcaatgtctg ataccgacaa actgctacac
    10261 tcacttaaac ggatgcctga cccgtaaagg acttccagta aagcgcggtt cctcaaatcc
    10321 aaaggactat ctcctgctac gctgtcaaaa agtgcttgca tttcattttc ataaaaaaaa
    10381 cgtggcagtc tcaaattttt ctttttgaga tgaacgtaag aaaatggatt ttcttttatc
    10441 acttcttctt tgagaagata ctgataaaac gaacgtaggc tggctatctt gcgactgata
    10501 gaatttctgc tatagctagg acgatgatca gctaatgcac tcaaatagac acgaaccgta
    10561 aaatattccg ccttcagcag atcagcgtct ccagattcct ccaaaaaatg tttgaaatgg
    10621 agaatgtctt cttcatatgc ttctttcgtt ttttctgaat atcctctttc gacaatcaaa
    10681 taacgcaaaa atttttgttc ccacgtggct tctgtcattt tctcaccttc cttttgtccc
    10741 agagaataat ttagcacagc gtttataaaa agacaaataa attgtcagaa tttaaccaat
    10801 atttctaagt ttgttcacaa ttggcgcaat tcctcacatt ttgtttgaga aattcttaag
    10861 aaaaacgcaa gaaaaagagc gagactatcc gtctcaccct cttcatggag tttccttact
    10921 catttgggat tgcagttttt tctaattctg tttttattgc ctcattagct tttaaagctc
    10981 gttgtgcaat tgcttcatat cttgcttttt tgtctcgaat gcgctctggc agttcaggaa
    11041 acagaccata gttggcattc atcggttgga aatgttttcc ttccgcatga gtaatgtagt
    11101 aagccatact tccaatagct gtttcttgtg gaaactcgac tggttccatt tcttttgcta
    11161 agcgtgcggc gttgattcca gcaagtagtc cgctcgctgc actttcaaca tagccttcta
    11221 caccagtcat ctgacctgca aagaacagat cttcgcgttt cgttgattgg taagtcggtt
    11281 tcagcaattc tggcgaattc ataaagctgt ttcggtgcat cacaccatag cgaacgaatt
    11341 cagcattttc taacccagga atcatccgga aaacacgttt ttgctcgccc catttcaagt
    11401 gagtctgaaa gcccacgatg ttgtataaag aagctgcagc attgtcttga cgcagctgga
    11461 taacagcata aggacgtttg ccagttttag gatcttccaa tccaactggt ttcataggtc
    11521 cgaaaagcat cgttttgaat cctcgctgtg ccattacttc aattggcata catccttcaa
    11581 aatatttttc tttctcaaaa gatttaagtt ctgccacttc ggcattgatt agtgcctcat
    11641 ggaaagcttt gaattcctct tctgtcatcg gacagttcaa gtaagctgct tctcctttgt
    11701 catagcggga cttcaaatat actttatcca tatcgatcgt agctttgtct acgattggtg
    11761 cagctgcatc atagaaataa aaaccatctg aaccattgaa tgctttgatg ctttctgcta
    11821 atggatgaga agtcaaaggt cctgttgcta ctacggtgat tccttccggt atttcagtaa
    11881 tttcttcgtt tatcactgtt actaatggat gctctttgac ttttcgggta attgtttcag
    11941 aaaaagaatc gcggtcaaca gctaaggcac caccagctgg cactgctgtt tcatctgccg
    12001 aagtgattac taccgaattc aatcggcgca tttcttcttt caataaccct actgcattag
    12061 ccaaactgtt tcctcttaat gaattggaac atactagttc agcaaaatta cttgtgtgat
    12121 gcgcctcagt cgatttgact ggacgcattt catataaacg gacagggact cctgcttgtg
    12181 cgatctgcca agcagcttca cttccggcaa gccctgctcc aacaacattt accattttca
    12241 atgaaagaac ctcttttcta ttatttttgg atgtcttctt cgtaatctcc atttacacag
    12301 actacttgtt taccgccttt gactttcttc tcgaccagat atccgtcaca ttttggacaa
    12361 tttctgccca ctggtttatc ccaagaagtg aattcacatt ctgggaaacg ggaacaacca
    12421 tagaatagtc tgtttttctt agattttcgt tcaatgactt gtccttcatg acatactggg
    12481 caagtcacac cgatttcttt tacgattggt ttcgtattcc gacaatcagg aaaattactg
    12541 caagcataaa acttcccata tttccctaat tttatcacca tcggatgtcc acagacatca
    12601 caatcgaatc ctgctggttc atctttgatt tgaatttttt cgattttttc ttccgcattc
    12661 gtcagttctt tttcaaacgg tttatagaaa cgatcaacca ctttgaccca cgcttccgtg
    12721 ccttcttcaa ttttatccaa atctccttcc atttctgctg tgaaatggat atcgacgatt
    12781 tgagggaaga attcacagat taatgaattg acaatttctc ctagttcagt cggctcaaat
    12841 cttttttgtg tcaattttac atagtagcgt ctttggatcg tatcaagtgt tggtgcataa
    12901 gtggaaggtc gccctacccc gttttcttct agtgcacgaa tcaacgtagc ctcactaaaa
    12961 cgtgctggtg gttgagtaaa atgttgtttt ggttcaatat caacagattg gactacatcg
    13021 ccttcttcaa gttctggaag aatattttct ttctcttctt tgccgtcatc tctaccttcc
    13081 acatacactt gcatgaatcc tttgaatttg atttttgaac cgttcgcaat gaagatgaca
    13141 ccattttgtt ctaatgtcac tttcattgta tctaaaatag ctggtgtcat ctgactagcc
    13201 acaaaacgtg accagatcaa tgtataaagt ttcaattgat ctttatctaa atattgtttg
    13261 agttcatctg gtgtacgaag cacactcgtt ggacgcacag cttcatgggc atcttgtgcg
    13321 ccttgagcat ttttgatctt tcggtgatca tgtctagaaa attcttttcc atacgtatct
    13381 tcaataaatg ctgcggcctc tgcttttgct gtatcggcaa tccgggtaga atcagtacgc
    13441 atgtatgtaa tcaagccgac tgttccttgt ttacccaaag tgattccttc atataattgc
    13501 tgtgctacca tcatggtttt tcttgttcga aagtttaact tacgggcagc ttcttgttgc
    13561 aggctacttg tagtgaaagg attcgcaggg tttcttttgc gctctttctt ttctactttt
    13621 ttaacttcga agtctgttcc atcaattcta gcagtgactt cttttacact ttgtgcatct
    13681 ggtaatttct ttttcttacc gtcgattccc cagaaatttg ctttgaattt cttgcgagct
    13741 tttttaaaat taccgtcgat gctccaatat tcttctggaa caaattctcg gatctctttt
    13801 tcacggtcaa taataatttt taaagcgaca gattggacac gtcctgcact taaccctttc
    13861 ttgaccttac gccagagaat aggactgatc gaataaccaa cgattctgtc taaggtacga
    13921 cgagcttgct gtgcatctac taaatctaca tcgatcgttc ttggttcctt aaaagctgct
    13981 ttgactgcgt cttttgtgat ttcattgaag accacccgat ttttttcttt cagatccaac
    14041 ccaagtaggt acgctaaatg ccaagcaatc gcttctcctt ctctatccgg gtcacttgcc
    14101 aagtaaactt tttgagcttt ttttgctgcg gctttcaggc ttttgatgac atcgcctttt
    14161 ccgcgtatag aaatataatg gggttcataa ttgttttcaa aatcgatacc cattttactt
    14221 ttcggtaaat ctcttatatg gccaacactg gccattactt tatagtttcg tccaagatat
    14281 ttctcaattg tcttggcttt tgcaggtgat tctacaatca ctaaatattt atacgccatt
    14341 actgaatgga ctcctttcca ttgtcgtgtt cctactatta aaagaggatt catacaaatc
    14401 gtagctaatc atatccattc agaaaacgtt tgtcaagaaa agcaaatgaa aagtatggtg
    14461 ttattcttta aattctgcca agatatcttc acccgaaacc gtgcaaactg caccgtcttg
    14521 gatcaaatga tgacatccgg tagaatgttc tgttaaaata tttcctggaa cagaaaaaac
    14581 tgttcttcca agatccaatg cttgctgtgc tgttatcaga gagccgcttt ttttcttggc
    14641 ttcaaagaca catgttcctt tagacaaagc tgcgataatc cgatttctca ttggaaaaca
    14701 atgctttttc gggccggatt gaggaggata ttcactcaaa agaagatgat catttgcaat
    14761 cacctcttgc aagtcacgat tttcagcagg atagacttgc tctatccctg ttccaatgac
    14821 tgcgattgtc cgtccaccat gagagattgc tgcttggtga ccgtatgtat ctatcccttt
    14881 cgcgcagcca ctgacgatca catatccagc ttctatcact ggttttagta aataataaac
    14941 tgtttttttg gctaaagcag aagtgtgtct tccaccaaca acgcttaaca tgttacaaga
    15001 aagtaaagat agatcaccat tataaaacag tacaattggc ggataggtca agtgaagcag
    15061 ctgaggggga tatttgggat caaagcaagt gataaacgac tgtttcgatt ggattttgcc
    15121 ccagttagcg tgaatttcct gccaactttt gcgaaacagc tcccggtgtt tggttatttt
    15181 acctatctta atgatgtctt caaatccgat ttctttataa ggatactgca tcatccagtt
    15241 agcaagtgcc cattttcctt gactactgat tccttttgta taagcaagtt tcaataaatt
    15301 ttcttctatt tgatacatca taaaaccgcc tttcgtctct ctttctaata agatacgtaa
    15361 aagcggctgt tttttattgt aaatattttt ttacaggtgc aaaactttta cgatggattg
    15421 gcgtaattcc ttgtcgttca agtccttcta agtgttcctt tgttccataa cctgcattat
    15481 gcgagaaacc atatccagga taaagcacgt catattcttt catcaattgg tctcgatata
    15541 ctttagcaat gatacttgca gcagcaatcg acacagatcg tacatcccct ttgattattt
    15601 tttcctgctg aataggcaaa tccaaggtca tcgcatcgat caacaaatag cttggtacaa
    15661 tggacaattt ttctactgct tggatcatcg ctaatttact tgcttcataa atattcacat
    15721 catcgattgt ctgctgatca acgatcccga tcccgattgc cagggctttt tcttgaactt
    15781 gttgataaag aagttctcgc tttttctctg ataattgttt ggagtcattc agccctaata
    15841 tgatctcatt tttcggcaag ataactgcag ctgccacgac aggtccagct aatggtcctc
    15901 ttcctacttc atcaatgccg gcaatccatt ggtgaccttg tgcgtaccct tgcttttcaa
    15961 agcaaaacat ttcttcatat ttttccttaa ttaatgcttc ctttttcaat cgtcgttcaa
    16021 aagaagccag tgcttgctgc acgcctttgc gtggatcatt tcttaatgat tctagtgtag
    16081 gatcatctat tctctgtatg cttttcaatc gttctttgat actttggatg gattcagtca
    16141 tcactttttt gctccatttc ttcgcaacga tccagtgtgt atcgtccaag ttttccttga
    16201 cgaatatcat gcacgactaa ttcgctgcca cgatcatagt cttctttata cccacgtttt
    16261 gctgtaatat ccatcagtaa ttccggtgcc aatttttctt cttgctcgat tgataaacca
    16321 taacgatcgc tgattcttcc tgaataatat cgggcaaaaa agtctaatcc gtaaatcgct
    16381 aaatcatcta aatggacaag ctggtctttg atcgcgcctg tcagcgctag ttttttgccg
    16441 atttcttcat cttcaaattt gggccataaa atccctggtg tgtctaacaa ttccaactca
    16501 ttacctaaac gaagccactg ctgacctttt gttacgccag gcttatttcc tgtctgtgca
    16561 attttcttgc ctactagtcg attcatcaac gtagatttcc caacatttgg aatacctaag
    16621 accattgccc gaatagcgcg aggcttgatc ccccgagcag catctcttgc ccgtttttct
    16681 ttcaatgctt ctttagccgc aggtactaga cctttaattc ctttactttc ttgtgccgtg
    16741 atagctaatg caagatggcc ttgtttacga aaatattctt gccacagctt tgtctgttgt
    16801 ggatctgcca gatccgcttt attcaataaa acaaggcgag gtttttgctg aatgatctga
    16861 tctagcattg gattacgaga agataaaggt aatcgtgcgt ctaccaattc aaatacgatg
    16921 tccacatatt tcattttttc agaaacttct cgtcgggctt ttgccatatg tcccggaaac
    16981 cattggatgg tcattgttct ctccattcct ttcataccgg aatattcccc ggctttactt
    17041 gttttttctt tttcaatata aattcacttt tttattataa agaaattcca tctattagtt
    17101 aagtgaaagt gaaccagtcc tcattctatc acatcttcac ttttttga
//
LOCUS       DADXTH010000064        17007 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.064, whole genome shotgun sequence.
ACCESSION   DADXTH010000064 DADXTH010000000
VERSION     DADXTH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.064"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..126)
                     /locus_tag="IWI97_002057"
     CDS             complement(<1..126)
                     /locus_tag="IWI97_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBC2463551.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="IWI97_002058"
     CDS             complement(230..541)
                     /locus_tag="IWI97_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBC2463552.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="IWI97_002059"
     CDS             complement(543..740)
                     /locus_tag="IWI97_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBC2463553.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="IWI97_002060"
     CDS             complement(1134..2861)
                     /locus_tag="IWI97_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBC2463554.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="IWI97_002061"
     CDS             complement(2854..4047)
                     /locus_tag="IWI97_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBC2463555.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="IWI97_002062"
     CDS             complement(4234..4878)
                     /locus_tag="IWI97_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2463556.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="IWI97_002063"
     CDS             complement(4972..5106)
                     /locus_tag="IWI97_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBC2463557.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IWI97_002064"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IWI97_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBC2463558.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="IWI97_002065"
     CDS             complement(7237..7710)
                     /locus_tag="IWI97_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBC2463559.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="IWI97_002066"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="IWI97_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBC2463560.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="IWI97_002067"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="IWI97_002067"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBC2463561.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="IWI97_002068"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="IWI97_002068"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBC2463562.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="IWI97_002069"
     CDS             complement(11757..12896)
                     /locus_tag="IWI97_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2463563.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="IWI97_002070"
     CDS             complement(13205..13918)
                     /locus_tag="IWI97_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBC2463564.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IWI97_002071"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IWI97_002071"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBC2463565.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="IWI97_002072"
     CDS             15107..16639
                     /locus_tag="IWI97_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBC2463566.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="IWI97_002073"
     CDS             complement(16830..>17007)
                     /locus_tag="IWI97_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2463567.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DADXTH010000065        16832 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.065, whole genome shotgun sequence.
ACCESSION   DADXTH010000065 DADXTH010000000
VERSION     DADXTH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.065"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(42..851)
                     /locus_tag="IWI97_002074"
     CDS             complement(42..851)
                     /locus_tag="IWI97_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2463568.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1144..2580
                     /locus_tag="IWI97_002075"
     CDS             1144..2580
                     /locus_tag="IWI97_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBC2463569.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2750..3946
                     /locus_tag="IWI97_002076"
     CDS             2750..3946
                     /locus_tag="IWI97_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBC2463570.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4008..4193)
                     /locus_tag="IWI97_002077"
     CDS             complement(4008..4193)
                     /locus_tag="IWI97_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBC2463571.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4424..7738
                     /gene="dnaE"
                     /locus_tag="IWI97_002078"
     CDS             4424..7738
                     /gene="dnaE"
                     /locus_tag="IWI97_002078"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBC2463572.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            7906..8868
                     /gene="pfkA"
                     /locus_tag="IWI97_002079"
     CDS             7906..8868
                     /gene="pfkA"
                     /locus_tag="IWI97_002079"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBC2463573.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8942..10726
                     /gene="pyk"
                     /locus_tag="IWI97_002080"
     CDS             8942..10726
                     /gene="pyk"
                     /locus_tag="IWI97_002080"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBC2463574.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10761..11663)
                     /locus_tag="IWI97_002081"
     CDS             complement(10761..11663)
                     /locus_tag="IWI97_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2463575.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11849..12418
                     /locus_tag="IWI97_002082"
     CDS             11849..12418
                     /locus_tag="IWI97_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBC2463576.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12476..12655
                     /gene="rpmF"
                     /locus_tag="IWI97_002083"
     CDS             12476..12655
                     /gene="rpmF"
                     /locus_tag="IWI97_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBC2463577.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12825..14246
                     /gene="gndA"
                     /locus_tag="IWI97_002084"
     CDS             12825..14246
                     /gene="gndA"
                     /locus_tag="IWI97_002084"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBC2463578.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14439..15125
                     /locus_tag="IWI97_002085"
     CDS             14439..15125
                     /locus_tag="IWI97_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2463579.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15122..16627
                     /locus_tag="IWI97_002086"
     CDS             15122..16627
                     /locus_tag="IWI97_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2463580.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5421 a         2946 c         3534 g         4931 t
ORIGIN      
        1 aaaattgtca caacctcttt tttgtggaat ctaattctct attatagttt aagtagtttt
       61 tccagataaa cagctaaacc gtcttcctga ttgctcaaag gagtgacatc atttgcaatg
      121 cctttgagtt ggtcagtacc gttagccata gctactcccc aacctgcata atccaacaat
      181 tccaaatcat tatgctcgtc accaaaggca atgacgtctt tttgatcgat agacaggtag
      241 ttagcaatct cttgcacgcc ttttgctttt tggattcctt ttgaaacaat ctctaagatg
      301 gcagtaggtc caccccatgt ccgaacatcg acataactgc caaattgacg tgttaattca
      361 gcagatacag ctcctgcaaa acgtttgtct gttcggacaa gcaaagatgt aggatttgtc
      421 gtcaaatttt taggagaaag caaattcttc tctcctggac gagaagaagc aaaaattttt
      481 tcatcaaaga aatcaaaact gtcaataaaa aaagtatcac gattttcagc agcgataaaa
      541 tctaaattta gctgcttttt ttgcgataag atttcaaaag cgatttcacg attgatcaac
      601 gtttctcttt caccatccca gtgctgatta gggatgtgta ctaatgcgcc gttaaaatta
      661 accatcggtg ttgttaattc taattgttgg taaaactgat ggctcattcg gaatgggcgt
      721 cctgtagcaa tactgacatg atgaccggca ttgattgctt tttttagagt ctcttttgtt
      781 ttagatgtaa tcatcgaatc ttgattgagg gtagttccgt ccagatcgat ggcaattaat
      841 tttttattca ataagggtcc ttccttcctc aactagcatt tgttttagtc tagcatattt
      901 taggtaatta acaagagcaa tcagctattc ctaacaaaaa ctacagttat ggctatataa
      961 atagctaaac ataagctaaa cacgaacgtt aagtattatt ttcgcttttt ttgttaaaaa
     1021 aacgttgaaa agcgaatgaa acgtctatat aatttgaaat tgttcgatgg atttgcccta
     1081 ttttacttat tttttattat tatagttttc catcagaaaa aaattcaggg agtggaactt
     1141 tttatggaaa agtttttcgg tttaaagaaa aacaatacaa atgtatcaac tgaaataatg
     1201 gcgggagtaa cgacattctt tgccatgagt tatatcttat tcgtaaatcc gacgattttg
     1261 tcagcatcag gtatgccctt ccaagcagta tttctagcga caatcattgc atcgattatt
     1321 ggtacgctgg taatgggatt gttcgcaaat gtaccatatg ctcaggcacc tggtatggga
     1381 ttgaacgcat ttttcacttt tacggtcgtt tttgggcttg gttattcttg gcaacaagcc
     1441 ttggcaatgg tgtttatttg cggattaatc aatattttta ttactgtcac aaatattcga
     1501 aaaatgatta ttcgggcgat tcccgaaagt cttcaacatg cgattggtgg cgggatcggt
     1561 attttcgttg cgtatgtcgg aatcaaaaat gcaggattct tatctttttc agcagaccag
     1621 tcagctattt cgagctctgt cgttgaaggc ggaaaagcga caaatgttac gataaatggt
     1681 ggaatcgtcc ctgcgttagc caactttgac aacgctccta tcttattggc tgtgattggt
     1741 ttggtactga cagccatttt agttgtaaaa aatgtccgtg gggctattct tatcggtatc
     1801 gtagcgacca cagtccttgg tatttttatg ggtgttgttg atttaagttc cattgattgg
     1861 caaacgaatt cgttaggtaa ttcatttaaa gaattaggaa caactttcgg tgcagcattt
     1921 ggttctgaag gcatgcaatc tttattcagt gattcttcca agattcctca agtattgatg
     1981 acgatcatcg cattcagttt atctgatact ttcgatacga tcggaacatt tatcggtaca
     2041 ggacgccgca caggtatttt ctcaaaagaa gacgagatcg cgttggaaga tggtcgtggg
     2101 ttcaaaacaa aaatggataa agcactattt gccgatgcga tcgctacttc tgtgggggct
     2161 atctttggta catcgaatac aacaacttat gtcgaatctg cagctgggat cggggccggt
     2221 ggacgtactg gtttgacttc agtagtagtg gcagtgctat tcgcattaag tagtttattc
     2281 tctccactga tcgccattgt accaaatcaa gcaactgcgc cagcattgat tttagttgga
     2341 gtaatgatga tggcttcttt tgcagacatc aaatggctag atatggaaga agctttaccg
     2401 gccttctttg catcgatctt tatgggattg tgctatagca tttcctatgg gattgcagca
     2461 ggatttattt tctatacaat tgtgaaagta atcaaaggaa aatacaatca agtttctgtt
     2521 gctttgtgga tcgttgatat tttattcatt atcaacttca tcattctagc aacaatttaa
     2581 ctaaagagaa gggattggaa cagtatttgt tctgatctct ttttgtttta cataaaaaga
     2641 acgttacata taaaatgacc tttgcgggat ttttctcttt ttactggcga gatcgttgct
     2701 tgtttattcg aaaataggta tgataaaaga gacgaaagag ggatgataga tggagattaa
     2761 ttggagaaaa aatttagtga ttgcgtggat tggctgtttt ttcactggtg caagtattag
     2821 tttagttatg ccattcatac cagtttatgt tgagcaacta ggaacaccaa aagatcaaat
     2881 cgaattgttt tcgggactag caatctctgt gactgctttt gcatctgccg tggttgcacc
     2941 aatctgggga aatctagctg atcgaaaagg ccgaaaactc atgatgatcc gtgcagcagc
     3001 agggatgaca ttaacaatgg gatctttggc ttttgtacca aacgcgtatt ggcttttgat
     3061 catgcggttt tggaatggga ttttgtctgg atatattcca aatgccacag cgatgattgc
     3121 ttcacaggca cctagagaaa agagcggatg ggcgttagga acattgtcta ccggagcgat
     3181 tgctggaaat ctgatcggac cttcaatggg cggtgcactg gctcagtggt ttggtatgga
     3241 aaatgtattc ttgatcacag gggctttgtt gatgatcaca acggtattga ccattttctt
     3301 agtcaaagaa gatttccatc caatcgagaa aaaagatctg ataagcacca aagaaatttt
     3361 ttcaaaaatg gatcatctct ctattttgat cggtctcttc attacgacct tgattttaca
     3421 gataggaatc actacgatca gccctatttt gaccttatat attcgtgaat tgagcggaag
     3481 tacagaaaat atcctttttg tcagcggctt gattgtttcg atagctggtg tttccgctgt
     3541 cttttcttca ccgaaactag gaaaactagg ggataagatt gggaatcaaa aagttctaat
     3601 tggaggactc attttatcat tttgctgtta tttacctatg gcattcgtca ctacaccgct
     3661 ccaattaggt atacttcgct ttattttagg cttttcaact ggcgctttga tgccttctgt
     3721 caatacactg atcagtaaga ttacgcctca agaaggagtc agccgaatct atagctacaa
     3781 tcagatgttt agcaactttg gacaagtttt aggtcccatg ctaggatcga cagtcgcgca
     3841 tgcttataat tacagtgctg tttttattgt gacaagtttg tttgttctgt caaatattct
     3901 tctttcttta ttcaactttc gtaaagttct tcgcagcaga ttataatggt ggtgaggagt
     3961 agaaaataaa aaagagcttg agaaatctca agctcttggc tgttttttta cgaatgggca
     4021 gaggtttctt ttcttggcgc aactgcacca atcaaaagga taatgacacc cgcaatgaat
     4081 gaaatgattg ttgtcaaagt aaaatcatat gttccgcttg ttaatgcagc accaatataa
     4141 ccgactactt gacctagaac gacagcccag aataatgtaa cgatatatcg cataaagaac
     4201 acctcttttc tatcttaatt ttaccagatt ttctttgaat gtaaagaaac gagaccaaat
     4261 ctcaataaaa tttgagttct tgcgaaagaa ctcattccta cagattacaa gcaagcaaat
     4321 gaagtggaat aatcaacttc agcgcctgct atcttaatga aagtaaaagg atcaaacctt
     4381 ttaacgctag tgaattttaa cgaaaaaaaa ggcggaatgc aagatgtttt tcccacaact
     4441 ttatacaatt acttcttatt ctcttttaca aagtacgata cgtattcgag aatatgtaca
     4501 agaagcaaaa aaacaaggct acacttcatt agccataacg gataaagatg tcttatacgg
     4561 tgcacctgaa ttctatcgta tttgtgtaga agaaggagta cagccaatca ttggattaca
     4621 tcttagttat acttatgaag aaaaaaatta tggcttgctt gcgtacgcaa aagatgaaat
     4681 aggttatcag cagctgatga aactttccag ccgaaaaatg atccaagaaa aattggaact
     4741 tgcagaaatg acagaaaccg accatctagc gtttgttgtt ccggcaaatc attctgtttc
     4801 tgaggctttc aataaagagg ataacttttt cggtcattgg cagtttttac ttgaaaatgt
     4861 ttttactaga aatttttata tcggcttgac agaaaacacg cctgatcaat tgcctgaatg
     4921 gtatcagtat gtgataaaag aaaattattc gctttgtgca ttacatccca tcgctagttt
     4981 acaacaagaa gaactttttg cacttgaagt gatgaaacat atcaaagagg gaagccaaat
     5041 acctttagaa aatttatctg catctttacc actaacagca aatgtctatc ttctttcaga
     5101 acaagaaatg atcaaacgat tcgaaaaagc gggtctttcc caagctgtaa taggcgccca
     5161 gcgtttagct gaatcgattt catttgaatt ccagttccat cagaaattac ttccgcatta
     5221 tccagttcct aatggaaaac aagctggaga atatttaaga gagttatgct attcgcaagt
     5281 ggaacgccga atcactgact atacaaagga ataccaagaa cgtttggact acgagttatc
     5341 tgtcatccat caaatgggat tcgatgatta tttcttgatc gtatgggatg taatggcttt
     5401 tgctcataga aacaaaatcg ttaccggtgc aggtcgtggt tctgctgcgg gttcattagt
     5461 agcctatgtg ttagaaatca cagatgtgga tccattagaa tatgatttgc tgttcgaacg
     5521 ctttttaaac cccgaacgct ttacgatgcc agatatcgat ttggatattc ctgacaatcg
     5581 acgtgaagaa gtattgaatt atgttcgaga aaagtatgga caatatcata tggcacaaat
     5641 cgcaacattt ggcacaatgg cagcaaaaat ggttcttaga gatgcagcta gagtgtttgg
     5701 tttatcgcaa agtgaagcaa accgctggtc aaaagcaata cccaatcaat tgaaaatcac
     5761 attgacccaa gcttataaaa cttcgcatca attagtagaa ttagttcagg caagcgaaaa
     5821 aaaccaactt ttattcaaaa ctgcagtgat tttagaagga ttgccaagac atgtttcgac
     5881 tcacgcagca ggtgtagtta tcagcgataa gaatctactt gaactagttc cgctccaacg
     5941 cggttctgat gaagcttttc tgacccagta tacgatgaat gaggtagaag caatcggtct
     6001 tttgaaaatg gactttttag gactacgcaa cttatcgatt attgatttta ctttgaaagc
     6061 tgttaagcga gtagaagggt atgagatccg attaaaagag atcccgctaa atgatgcaaa
     6121 aacgctgctc ttgtttcaac gaggcgaaac ctcaggagta tttcaatttg aatctgctgg
     6181 tatccggaac gttttacgaa gattagatcc agaaaacatc gaagatattg ccgcagtcaa
     6241 tgcattgtat cgaccaggcc ctatgcaaaa tatagatacc ttcattgcca ggaagaaagg
     6301 aaaagaagcc attcgctacc cagatgattc actgatcccg attttgaaaa acacatatgg
     6361 agtgatcgtc tatcaggaac aaatcatgca gatcgcctcg actatggctg gatttacatt
     6421 agggcagtca gatattctgc gacgggcagt gagtaaaaag aaaaaagacg tattagatga
     6481 agaaaagtcg cattttgtat caggagcatt acaacaggga tattcggaaa aaacagcagc
     6541 gaccgtatat gaatatatcg aaaaatttgc aaactatgga ttcaatcggt cacatgcatt
     6601 tgcttattca ttcgtgggat tccaaatggc gtatctaaaa gcccatcatc caggtgcttt
     6661 ctttaccagc ctgatgaatt ctgttcggca taacacatca aaattgaaag aatatattgc
     6721 agaagcacgt aaaaataaac tcaaactttc ctcgccgtcc attaatcaaa gtaattccag
     6781 ttttgagttg gtcaataagg aagaaatcag attcggactg acagcgataa aaggtattcg
     6841 gcgtgatttt gtagaagata tattaaacga aagaaaacaa aatggcccgt tccagtcagt
     6901 ggatcaattc ctcatacgga tcgataagcg ttggctgaaa ttagagttac tgcaatcact
     6961 gacagcagta ggagtttttg atgaattatc tcctaacagg aaacaattga tgcttgatct
     7021 cgaaggaaaa atccaaaata tcgtttacag tggtggaagt cttgacttgc tgggaatcat
     7081 ggcattaaaa gaagaagaag tcgctgacta tacactagaa gaacgcttga agctggaaga
     7141 ggagtactta ggtatttatt tgtcaggtca tcctgcagaa gggtttgatc gtcttcgttt
     7201 agctaaacaa gttcagctga ttacagatat cgtccctaat caaccagtca atttattagt
     7261 gatgatcaag gaagttagag aaattcggac aaaaaaagga gaactgatgg cctttgttga
     7321 aggaaccgac agtagtggag aaatttctgt cacagttttt ccaactcttt atcgtaaata
     7381 tcgtaatttg tttgccgttg atcgagtgat ctttgttcaa gggcggagcg aaatcagcaa
     7441 atataatcag gaactgcaac taattggaga aagctttgct gatcctaacc agttagaagc
     7501 ccagtatcct tcacaaactt gctatttgcg tattacagaa gaacgggaag atcccgagat
     7561 aatgaaacaa ttacaagtga tttttcagaa acacccagga tatataccag tagttatgta
     7621 tcacgaaaaa acgcaacgaa aagtagttct gtcagatgct tattgggtgg atgacagcaa
     7681 gagtataaag cggcaactta tttatctgct tggcgaagat aatgtggttt tcaaataact
     7741 tacggtaaaa aatactactt gctttcattt tttttcattt ttttatccgt aaatcaagac
     7801 aagtgagaaa aataatgtta gaatgttagt cgtggttaac gcaaaaatgg ttgtaaacct
     7861 aagtcatact taggataaag tattttaatg aggtgaatgt ttattatgaa acgcatcgga
     7921 attttgacca gtggcggaga cgcacctgga atgaatgcag ctgttcgtgc cgttgttcgt
     7981 aaaggtattt acgaaggact ggaagtatac gggatcaatt atggttttgc cggtttagtt
     8041 gctggagata ttcgtcgtct tgatgtggca gatgtaggtg acaagatcca acgtggtggt
     8101 acattcttgt attctgcacg ttatccagaa tttgcaactg aagaaggtca attaaaagga
     8161 atcgaacaat tgaaaaaatt cggaatcgaa ggtctagtgg ttatcggagg agacggctct
     8221 tatcatggcg caatggcttt gacaaaacgc ggtttcccag cagtaggtat tcctggaaca
     8281 atcgataatg atattcctgg gactgatttc acaatcggtt ttgatacagc aatcaataca
     8341 gtactagaat caattgaccg tattcgtgac acagcgactt ctcatgttcg tacattcgtt
     8401 atcgaagtaa tgggacgcaa tgcaggagac atcgctttat ggtcaggtgt ggctggcgga
     8461 gcggatgaaa tcatcatccc agaacatgat tttgatatgg cttcagttgc taaaaaaatc
     8521 caagaaggcc gcgatcgcgg gaagaaacat tgcttgatca tcttggctga aggtgtaatg
     8581 ggcggaaatg aattcgctga gaaactttca gaatttggtg actatcatac acgtgtatct
     8641 atcttgggac acgttgtccg tggtggagca ccaagcgcac gcgaccgcgt gttggcaagt
     8701 aaattcggtg cttacgctgt cgaattgctt caagaaggaa aaggtggatt atgtgtcggt
     8761 atccatgaca acgaaatcgt tgcgtctgac atcattgata cacttgaaaa caacaaacat
     8821 aaaccagact tatcactata tgatctaaat aattcacttt cattttaaga gtgaaagaaa
     8881 ccaagctatt taagctgtgt acagctttaa ataaacaaaa ttgaatggga gagtttttta
     8941 aatgaaaaaa accaaaatcg ttagtacatt aggaccagca agtaactcag tagaaattat
     9001 ttctcaattg attgaatctg gggcaaatgt cttccgcttt aatttttctc atggtgacca
     9061 tgaagaacaa ttatcacgta tgactatggt ccgtgaagca gttaaaaaga caggaaaaga
     9121 tgtcggtatt cttttagata caaaaggagc tgaaatccgt acaactgttc aaggcacaac
     9181 agaagctgat tttggtcgtg caggatacat caaatttgaa gtaggagatc aaacacgcat
     9241 ctctatggat cctgaacatg taggttcaaa agaaaaaatc gcggttactt acccaggatt
     9301 atttgacgat gttcacgttg gcggacacat tttatttgat gatggtttga tcgatatgca
     9361 aatcattgaa aaagacgaag caaaccgtga attagtcgta gaagttaaaa atgcaggtat
     9421 gctaggatca cgtaaagggg taaatgcacc aggcgtttct attagtttgc caggtattac
     9481 tccaaaagat gctgacgata ttcgttttgg tttagacaac gatatcgact ttatcgctgc
     9541 aagttttgta cgtaaagcac aagacgttct agatatccgc gaaatcttag aagaaaaaga
     9601 catgactcat gttcaaatct tccctaaaat cgaatctcaa gaaggtatcg acaacattga
     9661 cgaaatcatc aaagtttctg acggtgttat gattgcccgc ggagatatgg gtgttgaaat
     9721 cccagctgaa ctagtaccaa tggttcaaaa acgcattatc aaaaaatgta atgctgctgg
     9781 tatcccagtt atcactgcta cacaaatgtt agaatcaatg caagaaaacc cacgtccaac
     9841 acgtgcggaa gcttctgacg ttgctaacgc tgtatttgat ggcacagatg caacaatgct
     9901 atctggtgaa tctgcaaacg gggactaccc agttgaagct gtttcaacaa tggcacgtat
     9961 cgaccaagaa gctgaaaatg cattatcaga attaggtaca ttccaattga acgaattcga
    10021 taaaacagat gtaactgaaa caatcggttt atctgtagct cgtgctgcaa aaaatctagg
    10081 cgtgaaaaca atcgttgctg caactgaatc aggatacact gcaaaaatga tttcaaaata
    10141 tcgtccagat gcagatatct tggctgttac ttttgacgaa cgcacaaaac gtggtttgat
    10201 gttaaactgg ggcgtttacc caacagttgc tgaaaaacca tcaacaactg atgaaatgtt
    10261 tgaactagca gctaaaaaag ctgttgaatt aggtttcgca tcagaaggcg atttaatctt
    10321 gatcactgct ggtgtaccag ttggtgaacg cggaacaact aacgtgatga aaattcaaat
    10381 gattggttct aaattattag aagctcaagg cgttggacaa aaatctgtcg ttgcaaatgc
    10441 agttattgct tcatctgctg aagaagctgt aaaaaaagca aaagacggaa tggttctagt
    10501 tgtaccatca actgacaaag aatatatgcc tgcaatcgaa aaagcagctg ctgttatcgt
    10561 tgaagaaggc ggattgactt ctcacgctgc agttgtagga attgcacaag atattcctgt
    10621 aatcgttggt gctaaaggcg caacaacaac aattacagaa ggtgaattga ttaccgttga
    10681 ttcacgtcgc ggcatcgttt accgtggcga aacaacagct atctaattta gcttaataaa
    10741 aaataaaaac agctcttctt ttacgaagag ctgttttttt gtgctgtaac gaattcatgg
    10801 ataacttgga ctacttgttc ttgaagaggc gtgacgatct gctgtgttcg ttttgccaat
    10861 gaaatcaaga agcgaggctg aacttcatct gaaagaggga ggcaaactaa atgatcttca
    10921 ggatggacag caatctctgc caaaaaaccg atacccacat tttctcggac catactcttt
    10981 aaaatcgtca agtcattgct ttgataaaca acttttggat gagtatgtgt ttgtttagaa
    11041 agcttagtga atgctgcggg gtggacataa tgttcgttca atagtacaaa ctgttcatct
    11101 gctatatctt tgaaagagac agtatcctgc ttggaaagag gatgtgtagg tggcacgacg
    11161 atcatgaaac gtttgtccaa aagtaactct gtttctaaat attgatccac gattggctgt
    11221 gttgagccta atacagctaa atcgatcctc ccttgtcgga tcatcccgta taagtcccga
    11281 gagcctccat cggtaatctc aatttgattc atcaattcat gcttgataaa gtaacttgac
    11341 agtttaggaa agtattcatt gccgataata ggaggcatgc caaaacgaag ctttttcttt
    11401 tgcaactgag tgatttcagc tcttgcagta gaaatctctt ggagaatggt ttgtgcatgg
    11461 agaaacagct gctttccaga aggcgtgacg attaattcgt gatgcgatcg atctcttgta
    11521 ataagtacgg tgtcaaactc tttttcaaga cgttgtaatg catatgtaat agtaggttgg
    11581 ctcacacgga aataattagc cactttcgta aaattttttt gtttgacgat ttggacatag
    11641 tattcgatat ctttcgtgtt catgaagatc atccgctcct ttaatggaat aatgattatt
    11701 gtaaatgatt ctgaggatga tagcaaacag aggctttcta ataaagattt ttctgttttt
    11761 ttatgaactg caaggtgtca agaaaatttc attgacaaca tgctttttgc cctgtataat
    11821 tgcttttgtt gcttaggagt gatgaaaaat gaaatggtct ttattggaat tgagaaaata
    11881 ccaggagaca ccgttaactt ttcatgagac gctcgacttg aaagccgctc tcatgaagcg
    11941 agacaattta atacttgatg ttgcgccagt ggaagtcgaa ggtttggtct cagttgataa
    12001 gaaaggctat atcgttcatt acactgtgca gacgactttg acagttcctt caacacgttc
    12061 actagaacct gttgaactgc ccatggattt ttctgtagac gaagtattca tgacagaaga
    12121 acaattccaa agtagagatg aacaaatgtc tgcggatgag attttgctaa ttgaaggtcc
    12181 attattgaat ttagcagagt cgattgaaga caacatctta ttggcgattc caatgcgggt
    12241 attgacaaaa gaagaggaag cagcaacaga acttccaaaa ggcaatgatt gggaagttgt
    12301 ttccgaagaa gaataccagc gcagaaaaat ggaagaagcc gagaataaaa ttgatcctcg
    12361 tcttgcaaaa ctatctgaac tttttgattc taaatcaaaa gacgacgata acaagtaaga
    12421 ttggaatata ttgtgtctaa tttagataca agactttaca aggaggtgta gcaaaatggc
    12481 agtaccagct agaagaacat caaaagctaa aaaagcaaaa cgtcgtacac actacaaatt
    12541 gacaatcaaa gggttaaacg aatgtccaaa ctgtggtgaa atgaaaaaaa gccatcacgt
    12601 atgtgcaaat tgtggttact atgatggtaa ggacgtaatg tcaaaagaag cttaatacat
    12661 tttaattaga aaatgtaaaa cctcaaggct aaccatggag ccttgaggtt tatttttttg
    12721 tagtcggtaa ataaaaaata tgttgttctt tagtttgcaa tgtagccgtg ttaagctata
    12781 atgtaagaga ttagggagaa atcccgtatg taggaggagt tcatatgtca aaacaacaaa
    12841 tcggcgttgt cggtatggcc gttatgggta aaaatctagc cttgaacata gaaagccgcg
    12901 gctattcagt ggctttattc aatcgtacag gggctaaaac aaccgctgta gtagaagaac
    12961 acccagataa aaatttcaaa gcaacatata caatcgaaga atttgtagaa tctatcgaga
    13021 aaccacgtcg gatcttgcta atggtcaaag caggaccggc aacagatgca acgatccaag
    13081 agttgcttcc tcaccttgat aaaggcgata ttttgattga cggaggaaat acattcttta
    13141 aagatacgat gcgtcgtaat gaagagttag ccaactctgg catcaacttt atcggtacag
    13201 gcgtttccgg tggtgaagaa ggagctttaa aaggaccttc aatcatgcct ggtggacaaa
    13261 aagaagccta tgcgctagtt gcaccaatct tagagcaaat ttctgcaaaa gcagaagacg
    13321 aagcaccatg tgtcacatat attggcccaa atggagccgg acattatgtg aaaatggtac
    13381 acaacggtat tgagtatgga gatatgcaac tgattgctga atcttatgac ttaatgaaga
    13441 atattttaaa cctttcagta gaagaaatgg ctgatatctt taaagaatgg aatcaaggcg
    13501 aattagacag ctatttgata gaaatcactg ctgacattct gacacgtaaa gacgatgaag
    13561 gaacaggtaa accagtagta gatgtgatcc ttgatgcagc tgggaataaa ggaacaggta
    13621 aatggacaag ccaaagcgcg cttgacttag gtgtgccact tccattgatc actgaatctg
    13681 tatttgcccg ctatatttct gcttataaag aagaacgcgt acaagcaagc aagattttat
    13741 ctcgtacgaa cgattttgag ttcacgggag acaaaaaaga attggtcgaa aaaatccgag
    13801 aagcgttgta tttcagtaaa atcatgagtt atgcccaagg atttgctcaa ttacgtgtcg
    13861 cttcaaaaga atttgattgg gacttgccat ttggtgaaat cgcaaaaatc tggcgcgctg
    13921 gatgtatcat ccgagctcga ttcttgcaaa aaattacgga tgcctatgac aaaaatcctg
    13981 agatcgaaaa tttattgcta gatgattact tcgttgaaat cacgaaaaaa tatcagcaat
    14041 cagtcagaga cgtcgttgcc ttggctgtcc aagctggcgt acctgtaccg actttctcat
    14101 cagccatcgc ttacttcgat tcatacagag cagaacgtct gcctgcaaat ataatccaag
    14161 cccaacgcga ttactttggc gcacatacct atgaacgtgt cgacaaagaa ggaatcttcc
    14221 attactcatg gtaccatgaa gaataatagt ctttcggctg ttaggaaatg agaaaataaa
    14281 aagaggacga aagagctgaa gtcgattgtt atgagtagac ttcagctctt ttttaaaatt
    14341 gctatccatg ataaaatgac tatagatttt taagcatatt atcggtataa tgatagagtt
    14401 tttaaaaagc cagtagaata gaaaggataa aataataaat gagcaatatt ctaatcattg
    14461 aagatgaaaa gaacttggca cgatttgtcg agctagagtt gaagcatgaa gggtataacg
    14521 cagaagtcca ctacaacggc cgaactggtt tagatgcggc attaaataat gagtgggatg
    14581 ccatattgtt ggatctgatg ctcccagaat tgaacggttt agaagtctgc agacgtgtac
    14641 gccaagtcaa aaatacacca atcattatga tgactgcaag agattctgtg atcgatcgtg
    14701 tatctggact tgaccacgga gcagatgact acatcgtcaa accatttgct atcgaagaat
    14761 tattagcacg tcttcgtgca ttgttgcgtc gaatcgatat tgaaggcgat aaaaatgtgg
    14821 ctaaacaaac tactattact tatcgcgatt tgactatcga aaaagaaaac cgtgtcgtac
    14881 gtcgcggaaa tgaagtaatt gaactaacaa aaagagaata cgagttgctg cttactttaa
    14941 tggaaaacgt gaacgtggta ttagcacgtg acgttttact aaataaagtg tggggatacg
    15001 aaacagaagt tgaaacaaac gtagtggacg tatatattcg ttatcttcgc aacaaaattg
    15061 acgttcccgg ggaggaaagc tatatccaaa ctgtacgcgg aacaggttat gtgatgcgct
    15121 cgtgataaag ccgaaaaaat cggaaaaaag agaactgaaa gggccctcct tgacaatcaa
    15181 gtgggccttt gcgagttctt tcttcatatt cgttgtgttt acaattttcg ctgtcattac
    15241 ctataaatca tctatcaatc tgattgtggc aaaagaaaga aacaatgtag agcgtacgat
    15301 ttcagaagta gcttctcgtt tggctaactc ggatgaagaa ctgacattag taaatacgta
    15361 tcgtaatctg acgaaagcga caaatgacgg agatgaggtc gtagatcata cttcactggt
    15421 cgaaggaaac ttaatgaaga tcgattcctt tatagctgag ctagggcagc catcaatgga
    15481 tctttatgtt tatgatttgg acgagaactt gatttttaaa acccatgaaa acagtcgaaa
    15541 cctgattcag actacacgta aagttccaac gatcgttaca ctagaaggaa aaactggctt
    15601 tttatctatc cagcctatat attctaaagg tacaagagaa aagatcggct atgcgcaaag
    15661 cttctatgaa ttatcttctt tctatgatat tcgaaataag ctattattga ctcttattgt
    15721 tttagaagtt gtttctttga tcttaagtag tattttaggt ttctttttat cttcttattt
    15781 cttaaaacca ctcaaggtac tcagagatac aatggatact atacgtaaag atccgcaatc
    15841 agatatccac atgcctgaaa tcgacacaaa tgatgaattg gcagatttag ctgaaatatt
    15901 caatgagatg cttgaccgta tgcgtttgta tattgaacaa caagagcaat tcgtggaaga
    15961 cgtatctcat gagttaagga cacctgttgc aattattgaa gggcatctta gtctgctaaa
    16021 tcgttggggg aaagatgatc ctgagatatt agaagaatca ttaagcgcca gcatgcaaga
    16081 aattagccgt atgaaaagct tagttcaaga gatgctagac ttatcaagag cagaacaagt
    16141 agatgttcat tatgcgaatg aaacaactaa aggaaaagaa gtcacctatc aagtttataa
    16201 taatttcaag atattatatc cagactatgt gatcacttta gatgatgatt tgacaaaaga
    16261 agtgaccctg caaatttatc gtaatcactt tgaacaatta atcattatca tattggataa
    16321 cgcagtaaag tattcaacta cacgaaaaga agtccatatc tctatttctt ccactctgaa
    16381 tgaatttgag atcgcgattc aggactttgg cgaaggaata cccaaagaag accttaataa
    16441 gatcttcaat cgtttttatc gagtggataa agcaagagct agaacgaagg gtggaaatgg
    16501 tctggggttg tctatcgcta aacaactggt tgaaaactat aaaggacgta tcttagcaga
    16561 aagtgtagta gggcaaggga caatttttag aatctttatt cctatcgcaa aggcagaaga
    16621 caagtaaagc atgtgcttta cttgtttttt tatatatcca acaaagaata aggtacaaaa
    16681 tacaagcaag ttagaaattc agggatgtca ttataaaaaa aactagcgaa caattaaata
    16741 gaataacatc cgttaattaa acgtaagtag cttttagatg aacgaaaaaa caatgaaatg
    16801 ctgtaatatt cgtgtttaaa aataataaat aa
//
LOCUS       DADXTH010000066        16761 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.066, whole genome shotgun sequence.
ACCESSION   DADXTH010000066 DADXTH010000000
VERSION     DADXTH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.066"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(42..1607)
                     /locus_tag="IWI97_002087"
     CDS             complement(42..1607)
                     /locus_tag="IWI97_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBC2463581.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(1600..3051)
                     /locus_tag="IWI97_002088"
     CDS             complement(1600..3051)
                     /locus_tag="IWI97_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBC2463582.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(3065..4081)
                     /locus_tag="IWI97_002089"
     CDS             complement(3065..4081)
                     /locus_tag="IWI97_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2463583.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            4301..5389
                     /locus_tag="IWI97_002090"
     CDS             4301..5389
                     /locus_tag="IWI97_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBC2463584.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            5443..6969
                     /locus_tag="IWI97_002091"
     CDS             5443..6969
                     /locus_tag="IWI97_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBC2463585.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            6971..8176
                     /locus_tag="IWI97_002092"
     CDS             6971..8176
                     /locus_tag="IWI97_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2463586.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            8433..9410
                     /gene="guaC"
                     /locus_tag="IWI97_002093"
     CDS             8433..9410
                     /gene="guaC"
                     /locus_tag="IWI97_002093"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBC2463587.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            9674..11098
                     /locus_tag="IWI97_002094"
     CDS             9674..11098
                     /locus_tag="IWI97_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBC2463588.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            11559..12668
                     /gene="pdhA"
                     /locus_tag="IWI97_002095"
     CDS             11559..12668
                     /gene="pdhA"
                     /locus_tag="IWI97_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBC2463589.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            12671..13648
                     /locus_tag="IWI97_002096"
     CDS             12671..13648
                     /locus_tag="IWI97_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBC2463590.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            13673..15316
                     /locus_tag="IWI97_002097"
     CDS             13673..15316
                     /locus_tag="IWI97_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBC2463591.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            15324..16730
                     /gene="lpdA"
                     /locus_tag="IWI97_002098"
     CDS             15324..16730
                     /gene="lpdA"
                     /locus_tag="IWI97_002098"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBC2463592.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT         5401 a         2920 c         3700 g         4740 t
ORIGIN      
        1 tattgcaaat ggaaagagta tcattgtacg gatttcgttt attagttatt tttgttttta
       61 aggaaaagct gtgtccgata ttcttttgga gaaatccctt gttttttctt aaataaataa
      121 ctgaaatagt tagggtcatt ataaccaatt tcaaaagcaa tatctgaaat acgatagtct
      181 gattgctgca ataatttttt tgctaattcg attctttgtt ctgtcagata atcgatgaat
      241 gtttggttaa acgattgaga aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt
      301 tgagcgaccc aagttaagga catatctgga tcggtaaaat tttctttgat aaaatccaat
      361 gcatggttca aaattggctg atatttgttc cttgaaggct gtgagggcag agcagttaag
      421 aaagaaatga acatataaac tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag
      481 cgttgactgg tggatgtgca atgaaccatt tcttgcggga ttttcttttc tttcaggcag
      541 acatgctgga gtagcttagt caagacaaat aaacgatata atcgtgttcg agttggtgtg
      601 ccttgtggtt tcgtcaagtg ttcgatgtac ttagatactt cgtttttcga taatgaagac
      661 aaatcaagtg agaggtcaaa gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc
      721 aaatcctcat agctgataat ttgttcttta agggaactgg cggtttcaag tatcatctgc
      781 gtgatctcaa aagatgcagg taattcgctg attctttctg cgacaggacc aatggcgaca
      841 gaaaaattat ttttttgtcc cttttctaat tcatggacca acgtttgggc tacctgataa
      901 ctggtctcga tcacattttc ttcaagagaa tcaactatta aaaatttgat gaatcgagag
      961 gaaacagcag agaagatcac ttgttgattc tccctaaata aaaacttcaa ataatcactg
     1021 agatgagcgt aatcttcaaa gtctgtactg acttggtttg ctgctaataa tacggtcatt
     1081 tttttccctg ctaaagagcg attgaaagat tcagcaagtt tcaatgtttc actgatcgta
     1141 agtgagcctt tgaacaaacc gttcaagaaa tgattttttt tcaattcgaa aacaagatca
     1201 gaagaagtgt gcgtttcttt ttgctgcaaa tctaaagcag ttactgcttt ttctattgct
     1261 tgtttcaatt cgctactttt aatcggttta agcagataat catctgcctg tacctggatt
     1321 gctcctttca tataatcgaa atcgtcgaat ccactaataa atatgagacg agtccaagga
     1381 agtagtttcc ttgcttcttt tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca
     1441 gtcagaatga tgtcgggctg taagtcgata attgaagaca gtgccatctc accatctcca
     1501 gcttcgccga cataagtgat tggataataa gaaccaagga cagttatttg ttcccgcaag
     1561 ttattgcgaa tgaggtattc gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc
     1621 aattgttttg ggagatagag ggatcatcag tgtgacagtt gttccttctc ggtatttgct
     1681 gtcaatcgtg ataggttttt gatcatttcc gtaataaaga aggagacgct tgtttacgtt
     1741 gtaaagtccg tagccgatac tgaaatctga atcgatccct ttggctaact ctttacgtat
     1801 ttctgccagt ttttctgcag ggatgccaat cccgttgtct ataactttga attggatcat
     1861 ctcatcaatt ttgcatgtgg taatacgaat aaatcctttg tgacggacgt acttcgttcc
     1921 atggtataca gcattttcaa tcaatggctg caagatcatt tttaaaatag aaacaggaaa
     1981 aagtgtttct ggtatatcga tcttatagtc aagcatatgg ccgtatctta tttttaagat
     2041 gactagataa tcattgatat ggctaatttc tttttcaaga ggaatccagt cttgtccttt
     2101 gcttaaagac gtacgaaaaa agccggaaag tgcatgggtc atagtcagag cttcttcaaa
     2161 gcagtcttgt tcgatcaaag aaataatggc atccaaactg ttataaataa aatggggagt
     2221 aatctgagct tgcaatacac gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt
     2281 ctcccgtaaa gactggttta gatcttccgc cattttattc atgctagcgg taagtgtttg
     2341 taattccagt gtttccggca gggttaaacg aaagccaagg ttccctgaag aaagttgttc
     2401 tgacatctcg atcagctgat tgatcggttc ttggaccttt ctctctaaaa agcgacgatt
     2461 ctttttcgca tagaaaaaaa tggtactaag aactccaagt tccactattg ttaatagaat
     2521 gagcgagcgg acaagtgtcg tatttttttt gcttgctaag tcgatctcca cacgaacaaa
     2581 atcttgtaag atatctgaca gcagttgagt gacagaatct acctgattga tgatctcttc
     2641 attggtatcc ataaaatctt ttgccaagat attattgatc agctcgtttt gataatcttc
     2701 cagtgtatcg atcgtacgta gcgatacatc taacgtacta atttctttaa gtgtatttgt
     2761 attatttttg atctctcgaa tatcattcct aagttcttct aggatatttt cctcattgaa
     2821 ctcatctacg gttacttggc cagaaatcaa gtcccacata ctttccaaaa ctttttgatc
     2881 aaccttcaaa gcaattgcat ctgctttttg gatatttgcg atcgtctgtt gataaatcaa
     2941 aagctgttgc gaatataaca gtatgctcag tagcaaaggg ataacagcaa atagaagcat
     3001 ggctttgttt gcttgggcaa aaatctgttt tatgcttaaa ttttttgtca tgatacttct
     3061 ccagtcaata atttctggtt gggatgttag ctatattctg ctgggaaaag acagtctcgg
     3121 aaataaaaat atctttaggt aatgtttttc ctgtttgaat atttccaaaa taacgagtaa
     3181 cggtattggc tacataccag ccgacatcag gtttacactc gactacacaa ttgacgattc
     3241 ctgagcgtag cttctcgatc atatcttctt gtccatcaat agttacaatg atcaaatctt
     3301 ctccaggttt gattgatgtt tcttttatcg cagctaaagc accgtgagtc atttcatcac
     3361 tatggctgta taatacatcg atttcggata aatcatttgt ttctaaatat cgtttcatct
     3421 ggtcttttcc tttcatccga ataaaatcac cgacaattcg gtgaacaata gtcatcggag
     3481 gtttatggtc gatggttcca tcttttttta gggttggttt acgattgatt gattccaaaa
     3541 atccttcgga gcgtagcttg gtcggtgtgg agttctctga accagcaagt tctaggattc
     3601 tgatttcctg tttctttgaa tttgtgaagt gattgctgac aaataaaccc gcccgatttc
     3661 cttctgcttt gaaactgggg ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa
     3721 tatgtctgtc gacaagtatg acagggatcc ctgctcgctt tgcttctttt aatacagagt
     3781 cccacccgga ttcttgcaaa ggagtaaaaa tgatcatatc cactttatag gcaatgaacg
     3841 tccgtatatc ttggatttgc cgttcctgat tcatgtagcc atttcggtag agaacttggt
     3901 aatcttcttt ttcaagcgca gtgataattg attcagtatg ctttttccgc cagctactct
     3961 ccgttcctga ttgcgaaaaa ccaacgacta gtgggtcagg tgcttccttt gtattaggtg
     4021 tacaccccat catttggtaa gcgattacaa caagggctaa aaaaaataaa acacgtttca
     4081 ctttaaatca ctccatctgg atttgttagt actatcttat cagataaaag ggaaaatcaa
     4141 agtcaaaagg aatcaatcga aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa
     4201 gtacacctaa aaaatttaaa gtaaatccca taaaaaaatc aatgttaacg ctttctttat
     4261 tagctatact cggtgtatca attgaaatgg gggataaaaa atgaaaaaca aatttagaaa
     4321 gattgttggg actatcgcta ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc
     4381 aggatctgca gatgatagta cggggtatgt aggaatcgct atgccgacca aatcagcaga
     4441 aagatggatc gctgatggga acaacatggt ctcggaatta gaaaaactgg gttataaaac
     4501 agatcttcaa tatggagaag ataaagtaga aaatcaagta gcacaaatag aaaatatgat
     4561 tacaaaaggg gtagatacac ttgtgattgc ttctatcgat ggttcggctt taactgatgt
     4621 gttagctaaa gcaaaagaag cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa
     4681 ttcagaaaat gtcgattatt acgcgacttt tgataatttt ggagtaggtg tatcgcaagc
     4741 agcttatatt gaagagcact taggattgaa agaaggaaaa ggaccattca cgattgaatt
     4801 atttggtggc tcgcctgacg acaacaacgc actaatcaat tacaacggag tgatgtctgt
     4861 tttacaacca tatatggaca acggtcagtt ggtcgtgcca tctggacaga cgagtttcag
     4921 tcaaatcgct acgcttcgct gggatggttc tacggcgcaa gcaaggatgg ataatctgtt
     4981 aagcgcaaat tatactgacc aaacattgga tgctgttttg tcgccatatg atccaataag
     5041 tttaggaatc atttcgtcat taaaaggcgt ggggtatggg agtgaaagta aacctttacc
     5101 tgtgattact ggacaagatg cgacagttgc tggcgttaaa tcgatcattg ccggggaaca
     5161 aacacaaacg atctttaaag acacacgtat cttagcaaaa aatacgattg aaatgatcaa
     5221 agcaatcagt gatggagaag aggttccggt caatgatact gaaacttatg ataatggggt
     5281 gaaaactgtc cctacgtatt tagcaaatac agtatctgtc gataaagata attatcaagc
     5341 agagctgatc gacaccgatt attataaaga atccgatctg aaaaattaag aggaaccaaa
     5401 caaggaacta aagagatcta agtcagagag aggataagaa ttatggcgga ttgtatttta
     5461 aaaatggcag atatcaccaa aaaattttct ggaatccgag cgttagattg cgtgaatctg
     5521 atgattgaac gtggggaaat ccatgcattg tgcggagaaa acggagcggg aaaatcaaca
     5581 ttgatgaatg tcttgagcgg cttgtatcca tatggctcct atgacgggga aatcttttat
     5641 gatggaaagc tgtgtcaatt taaagatttg aaagatagtg aagaaaaagg catcgtcatc
     5701 attcatcaag aattagcact aagtccttat ctttcaataa aagagaatat ttttctcgga
     5761 aatgaacaag cagaaagagg cgtgattgat tgggaactga cagaaaagaa aacatatgaa
     5821 ctgctgaata aagtcggttt gtctatcgac ccgaatcttc tagtatccca gattggcgta
     5881 gggcaacagc aactagttga aatagctaaa gcattatcaa aatccgtccg tctgttgatt
     5941 ttggatgagc ctaccgctgc attgaatgaa gaagaaagcg cgaatttgct gagattgatc
     6001 cgagagttga aagagcaagg tgtaacctcc atcattattt cacataagct gaatgaaatc
     6061 gtggatatcg cagatcgaat caccatttta cgggatggac agacgattga gacactagaa
     6121 aatgaaatga tcagcgaaga gcatattatt cgcggaatgg tgggcagaga tttagttgat
     6181 cgttatcctg aaagaaaacc ggatattgga gaagtttatt ttaaagtaga agactggacg
     6241 gtctatcacc ctcttgatca cgagcgaatc ataaacgacc acatcaactt gcatttgaaa
     6301 aaaggagaga ttgtaggaat agctggactg atgggagcgg gcagaactga attcgctatg
     6361 agtctatttg gacattctta tggaagcaag atatccggac gtatattaaa ggaaggggca
     6421 gagatatcag ttaagaccgt tccttcagcc attgcaaacg gactcgctta tgtctcggag
     6481 gaccgaaaat ctttaggact gaatctgctg atggatattc gggaaaacgt gacgctgagc
     6541 agtttggata aaataagtca aaaaggagta ttagataaag aaaaagaagt aactgaggca
     6601 gaaaatttcc ggaaaaagat gcgtataaaa acaaactcca tccatcaaat tgtcagtagt
     6661 cttagtggtg ggaatcaaca aaaagtcgtt ttatccaagt ggctgatgac acaacccgat
     6721 attctttttt tggatgagcc aacccgtggg atagatgttg gggcaaaata tgagatctac
     6781 acgattatcg aagaaatggc agcaaatggc aaagctgtct gtcttatctc ttctgaactt
     6841 cctgaaatat tagggatgtg tgatcgaatc tatacgatga acgaaggacg tattacagga
     6901 gaagtctcgc gagaagatgc agatcaggaa gtcttgatga aattaatgac gaaagaagag
     6961 gtgggttaag ttggaacagg caaaaaatct tacggataaa aaagagaaaa aactaaattt
     7021 gaaaagtaga gcagctgatc tttttggtcg ctacagcatg gtgatcattt tagcgggact
     7081 acttattgcg tttcaattga tgacggatgg tattttctgg cgtccattga atataactaa
     7141 ccttgttctg caaaatagcc atatattagt actagctgca gggatgttat tagttgtgct
     7201 cttaggtcat gtggatttgt cggtagggtc agtgatggcg tttgttggtg caattgcggg
     7261 catgatgatg gtcaatcgcc aaatgaaccc gtggatgaca gttatcgcat gtcttgcagt
     7321 aggcgggctg atcggcgcat ggcaaggttt ttgggtagct tatgcaggga taccggcgtt
     7381 tatcgtgact ttagcaggat tgctgatgtt tcgtggactg acacaagttg ttctaggagg
     7441 ccaatctcta gcaccttttc cagctgtttt ccaaaaaatg tctacgggtt atttaccaga
     7501 cttaagcgaa ggaagcagcc ttcacttctt gacattgatt ttaggaatga tcatcacgat
     7561 cgtgttagtt gttggtcaat ggcgaacaag aaatcgtcgg aaaaatcatt tatttgaagt
     7621 ggaatctcca acagctttct tggtaaaggc ggtatttaca gcagtcgttg tactaggaat
     7681 aacttatatc tttgcctctt atcaagggta tccgattatt ttgatcattc ttggtttgat
     7741 cgttgcagca tatgggtttt tgacaaataa aacagtagca ggtcgacaga tttatgcgac
     7801 gggcggaaat cgaaaagcgg ctgaactatc agggatcaaa acaaagcgaa tcacgttctg
     7861 ggtattcgtc aacatgggag tcatggcagc tttagctgga ttagttttag ctgctcgatt
     7921 gaatgcggca acacctcaag caggtacatc gatggaactg gatgcaatgg cggcagtata
     7981 ttttggtggt gcttcgactt ccggcggcat tggaacaatc atgggtgcga ttgtcggtgg
     8041 cttggtcatg ggtgtattga acaatggcat gtcgatttta ggcgtcgggg tagactggca
     8101 gcaggcaatc aaaggattga tcttgctttt agccgttgtg ctagacttgt ataataagaa
     8161 aaaacgaaca acgtaggaga acgccggtta ttcagaaaaa agactgtgac aagacatttg
     8221 tcacagtctt tttttcgcta aaagcgaaca ttcattgttt cgtttgtata gactaaaaat
     8281 aatatatgtt tttttaccat tcgtataaat tctctttttg ctcttgattt ttttgcttaa
     8341 ttgagttaaa ttacataggg cgctaaaaaa agccttgatt aaattaataa tgttcgtgtt
     8401 ttgctggatg tgaacatcag gaggataatg taatgaaagt atttgattac gaagatattc
     8461 aattaattcc taataaatgt atcgtaaata gccgttctga atgtgataca acggtcacat
     8521 taggtaaaca tacattcaaa atgccagtag tgcctgccaa catgcagacg attattgatg
     8581 aaacaatcgc agaattctta gcggaaaatg gttatttcta tatcatgcat cggtttgatg
     8641 aagcagctcg aattccgttt atcaaaaaaa tgaaaaagcg tggattgatt acctcgatca
     8701 gtgttggcgt caagaaagag gaatattcct ttatagagaa gttggcagaa gaatcgctaa
     8761 atcctgatta tatcactatc gacatcgcgc atggacatgc taactcagtt attgatatga
     8821 ttcagcatat taaaaaatat ttgcctgaaa cttttgtgat tgccggtaat gttggtacac
     8881 cggaagccgt ccgtgaattg gaaaatgcgg gcgcagatgc aacaaaagtc gggattggtc
     8941 caggaaaagt atgtattaca aaaatcaaga caggctttgg gactggaggc tggcaactgg
     9001 cagctcttag atggtgtgcg aaagcagctc gcaagccgat catcgctgac ggtgggatca
     9061 gaacacatgg cgatattgca aaatctgtcc gttttggtgc gacaatggtc atgattggtt
     9121 cattgtttgc cggtcacgag gaatctcctg gcgaaacaaa agtcgaaaat ggaatcgtgt
     9181 ataaagaata ctttggttct gcttcagaat tccaaaaagg cgaaaaacga aatgttgaag
     9241 gaaagaaaat ctggatccaa cacaaaggat ctttgaaaga tacactagtg gaaatgcaac
     9301 aagaccttca atcatctatt tcttatgctg gaggaagaga tctggaagcc attcgaaaag
     9361 ttgactatgt catcgtgaaa aattcgattt ttaatggaga cacaatctaa aaaataaata
     9421 aaatattagg atgttgtgaa aaagttgtcc tgcattaagt aataagaaca gccaaacaaa
     9481 aatagttctt tatatgtttt gtttggctgg aattattatc gcagatacaa gcttttgaac
     9541 atcattattc agaataaggg actgtgataa gatttttgtc acagtccctt atgttttgca
     9601 aaaaaagttt ttcttttttc tcgtaaattc ggtaaaatca aaagaaaaaa tagacaatag
     9661 aaaagagaag cttatgtggg aaaaagtaga gaaaaaacaa ggaccgattt atcgtcaaat
     9721 tatggaacag attatgagag aaatcgaaaa cggaaggatc aatcctggaa gtcaacttga
     9781 atcagaacga aaactagggc aactttttgg tgtgaatcgt tccaccgttg tccatgcatt
     9841 ggaagagctt agggagcttg gggtactaac gagcaaacgt ggcagcggat ggtttgttaa
     9901 tcagactgaa tgggggaaat tcgctgttcc aagaatcgat tggcggcaaa tgatctctcc
     9961 tcgttatgag cagacggact ggtatgagca aaaaataaga gaagcaaagc agatccaaaa
    10021 aacatcgttt cttgatttat acgctagcga gatgccagat gagcttttgc cgaattttga
    10081 gtttccttct tattctcttg aagaaatctt agcggaagaa aaagaaatgg atcttttagg
    10141 ctatactccg ttacgtaaca aaatcaaacg gtatttggaa agtaaatttg aattggcatt
    10201 ttcccccaac cagcttcttg tgactggtgg cggtcagcaa gcaatctttt tgattttaca
    10261 aacgattctt tcagctggag aggggatagc tgtagagtca ccttcttttt tttaccgatt
    10321 acctcttttc aaagcttcag gaacgcgttt atttgggata ccgatggatg aagaagggat
    10381 cgacttagaa aaactgaaag aatcgatcca taaaaataaa ttgaaagcag tgcttatcaa
    10441 cccgaatttt caaaacccta caggaaaagt gatgagtcaa aaacgacgag aaaagctagt
    10501 agaattgtgt cgacagtatc ggcttccaat tattgaagac gatgtattta cggatctttc
    10561 atttctaaac caagaagaag tccttcctat acgaacgatt gatccggata atgtgttata
    10621 cgtcggttcg ttatctaggg ttttaggaaa aacaacgaaa atcggttggg tgataggacc
    10681 tgagactttt atctcgcaac tagctcaagc gcaagaaatg atggagtttt cgatgaatat
    10741 ccttacacaa atcacagtgg ctaatgtatt taatgaagca attgatttga aaatgcaaga
    10801 tgtaagaaaa aagttgaaag aaaaaagccg actgataagc gcttggggag attcacaatc
    10861 gtttttcaag cttcattcaa ttttaggtgg ttactatgcc tggattactt gggatgggaa
    10921 aaaactagac cccgaactag cagaaaaagt agtagatatg ggtcttggaa ttgcaccaag
    10981 tctacttttt gggcaagata taaatggaat cagaatcaat tacagtaggc tggatgaaaa
    11041 ccaaataccg ttgttttatt caaagatgaa aattctagaa aagtggttaa cagaataatt
    11101 tcacaaagta aatagattaa agaatataac agcttggatt taaatttcaa gctgtttctt
    11161 ttttttcgga aaaagaatac gatataaaga aactaagaaa aaaccattat ttgtacgtcc
    11221 gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac tgtataacac caacgttttt
    11281 aaatagaaac agatggttgt aagtttgatt agaaaataga aaaaatgcag atgaaatata
    11341 aaacgctttc cttattgtga ttttattcac taatgctgtt aatatgcgga ttggcataat
    11401 ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt ctcataaatc catgataatt
    11461 tattaatgaa gacggttaaa tagtgataca gttttattca ctgtttcaat cgcagaatga
    11521 agagattttc ttatagatga gagaggaatg tgtgacaaat ggcaaacaaa aaagcaccga
    11581 tcgacttcca agcactgcta gatgaagtca atgcagattt cccagtatat caagcacttg
    11641 atcaagatgg aaacgtagtt aacaaagatt tagtacctga tttatccgat gatgaactcg
    11701 ttgaattaat gactcatatg gtatggtctc gtgtattaga ccaacgttca acagcgctga
    11761 accgccaagg acgcctaggc ttttttgcac caactgctgg acaagaggca agtcagctgg
    11821 ctagtgctta cgcatttgat aaagaagatg ttcttcttcc aggttatcgg gatgtacctc
    11881 aactagtaaa acatggttta cctttatctc aagctttttt atggtctcga ggacatgctg
    11941 caggtaattc ttatccggaa gaattgaaag ctctaccacc acaaattatc atcggtgcac
    12001 aatatgtaca agcagccggt gttgcattag gtttgaagaa acgaaacaag aaaaatgtcg
    12061 tcttcactta tactggtgat ggcggttcat cacaaggtga tttttacgaa gcaatcaatt
    12121 tcgctggcgc ttaccatgca aatgccgtat tttatatcca aaacaacggg tatgcgatct
    12181 ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc acaaaaagct gtcgcagctg
    12241 ggattcctgg tattcaagtc gatggaatgg atccgcttgc agtatatact gtatctaaaa
    12301 tggcacggga atgggcgatc agtggaaatg gaccagtcct aatcgaaaca ctgacttatc
    12361 gttatggacc acatacacta tctggtgacg atccaacccg ctaccgtaca aaagacacag
    12421 atgacgattg gcagaaaaaa gatccattga tccgcttccg taaatatttg acggaaaaag
    12481 gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc aactaaagaa gaaatcaaag
    12541 cagctattgc tgaagccgac aaagtaccaa aacaaaaggt ttcagatttc ttgaaaaaca
    12601 tgttcgaagt atctccacaa tcaatcaaag aacaaattgc aatttatgaa gcgaaggagt
    12661 cgaaataatc atggcacaaa aaacaatgat ccaagcaatc acagatgcct tagcacttga
    12721 actagaaaat gacgagaatg tcgttgtttt tggtgaagat gttgggaaaa acggaggagt
    12781 tttccgggca actgaaggat tgcaggaaaa gttcggagaa gaccgagtat tcgatactcc
    12841 tttagctgaa tctggaatcg caggattgag tttcggtctt gcgttagaag gatttcgtcc
    12901 agttcctgaa atccaattct ttggttttat tttcgaagca atggacgaag tagtagctca
    12961 aatggctcgt acaagatatc gaatgagcgg cactagaaat ctacccatca caattcgttc
    13021 gccatttggt ggcggtgttc atacaccaga attgcattcg gataatttag aaggtttgat
    13081 cgctcagtct cctggtattc gagtagtgat tccttccaac ccttatgatg caaaaggact
    13141 attgatttct tctattagaa gtaacgatcc ggttgttttc ctagaacaca tgaaactata
    13201 tcgctcattc cgagaagaag tgccggatga agcttacgaa gttcctttgg ataaagcagc
    13261 agttactcgt gagggaacag atgtatctat tatcacatac ggagcaatgg tgcgtgaagc
    13321 aatcaaagca gcagacaatc ttgcaaaaga aaatatatct gttgaaatca ttgatcttcg
    13381 tacagttgcg ccattagatg tagaaacgat cattcaatcc gtggaaaaaa ctggtcgcgt
    13441 agttgttgtc caagaagcac aacgacaagc gggcgttgcc gcgcaagtag tttctgagat
    13501 ctcagaacgc gctatccttt cattagaagc cccaatcgga cgagtttctg cacctgatac
    13561 agtcttccca tttggacaag cagaaaatgt ctggttgcca aatgcaaaag atatcgaaga
    13621 caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga cgaaacaaag tcatggctta
    13681 tcaatttaaa ttgccggata tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt
    13741 cgtcaaacca ggagatacga tcaatgaaga tgatacatta ctagaagttc aaaatgacaa
    13801 atcagtagaa gaaatcccgt cacctgttac aggaacggtt aaaaatgtga ttgttccaga
    13861 aggtaccgta gcaaatgttg gggatgtatt agtagaaatc gatgcaccag gacacgaaga
    13921 caatgaaggg gacagtggtg tagccgcaga atcccaaact ccagctaaac cggctgctga
    13981 acccactgtt gatacagaat cagctggatc gtcaagcgaa ggagtcttcc aatttaagtt
    14041 accggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg tcaaaccagg
    14101 agatacgatc aatgaagatg atacattact agaagttcaa aatgacaaat cagtagaaga
    14161 aattccgtca cctgtcacag ggacagttaa aaacgtgatt gttccagaag gcactgtagc
    14221 aaatgttggg gatgtattag tagaaatcga tgctcctgga cacaatagtg caccgtcaac
    14281 tagtgcgcca tcagctgaag caccaaaaga aaaagtggaa acatcaggtt cagcaagtgt
    14341 cgttgaagct gccgatccaa ataaacgtgt attggctatg ccatctgttc gtcaatttgc
    14401 ccgtgaaaaa gatgtagata tctcacaagt aactgcaact ggaaaaggtg gcagagtaac
    14461 aaaagaggac atcgaaaact tcttggcagg tggtccaagc agtgcaccag cgaaatcaga
    14521 agcgccagaa gcggcagcac cgaaagaagc agctcctgca gcagaaagca aaccagctgc
    14581 accagcaaaa ccattcaagt ccaacttagg agacttggaa gaacgtgtag caatgacgcc
    14641 aacacgtaaa gctatcgcta aagcaatggt caacagtaag catacagctc cacacgtgac
    14701 attgcatgat gaagtagaag tgtctaaact atgggataat cggaaacgat ttaaagaagt
    14761 tgctgctgca aatggtacga aacttacttt cttgccatat gtcgtgaaag ctttaacagc
    14821 gactgttaag aaatacccag tattgaatgc atctatcgac gatgcaaacc aagaaatcgt
    14881 ttataaacat tactacaata tcggtattgc aactgatacg gatcacggac tatatgtacc
    14941 aaatgtgaaa gatgcagacc gcaaaggaat gtttgctatt gcagacgaaa tcaatgagaa
    15001 agcaaaatta gctcatgacg gcaaactatc tgcagaagat atgcgcaatg gaacaattac
    15061 gatcagcaat atcggatctg taggtggcgg ttggtttaca ccagtcatca actatccaga
    15121 agtagctatt ttaggtgtag gaacgattgc acaacaacca atcgtaaatg ctgaaggcga
    15181 aatcgttgtc ggtcgtgtga tgaaactgtc attaagtttc gatcaccgta tcgtagatgg
    15241 agcaactgcg caacaagcaa tgaataatat caaacgtctt ttagctgatc cagagctatt
    15301 aatgatggaa ggatgataaa caaatggtag ttggagattt tgctgtagaa ttagatacag
    15361 ttgtaattgg tgcgggacct ggtggctatg tagctgccat ccgcgctgct gaaatgggac
    15421 aaaaagtagc aatcattgaa agagagtata ttggcggtgt ttgtttgaat gtcggctgta
    15481 ttccttcaaa agcactgata gctgcaggac accattatca agagtcacta gattctgaaa
    15541 tgtttggagt aactagtgaa aacgtcaaac tagattttgc aaaaacacaa gaatggaaag
    15601 aaaacaaagt tgtccatacg ttgacttctg gtgttggttt cctattaaaa aaacataagg
    15661 ttgaaacaat cgaaggagaa gcgttcttcg tagatgatca cacactacgc gtcattcatc
    15721 cagattcagc tcaaacttat tcgttcaata acgcaatcat cgcaacaggt tcccgtccaa
    15781 tcgagattcc aggatttaaa ttcggaggac gtgtgttaga ttctactggc ggattagcat
    15841 tgaaagaagt acctaagaaa tttgtgatca tcggtggcgg tgtgattggt gccgaattgg
    15901 gcggagcgta cgcaaactta ggagcagaag taacgattct tgaaggctca ccacaaattt
    15961 taccaactta cgaaaaagat ctagtaaaat tagtagaaga tgatttcaag aaaaaaggcg
    16021 taacagtcgt aacgaatgca atggcgaaag aagcagtcga taacggtgac agcgtgacag
    16081 tgaaatatgc agtggacggg aaagaagaat ccgttactgc tgattatgtg atggtcactg
    16141 taggtcgtcg tccgaataca gatgatatgg gcttagaaca agcgggtgtc gaagtaggag
    16201 aacgaggatt gatcacggtg gataaacaag gacgtacgaa cgtacctaac atctatgcca
    16261 tcggtgatat cgttccaggg gcggcattag cacacaaagc cagttatgaa gcaaaaatcg
    16321 ctgctgaagc aatctctggc aaaaaagtag ctgtagatta taaagcaatg ccggcagtag
    16381 ccttcactga tcctgaattg gcttctgtag gtatgacgat caaagaagca aaagatgctg
    16441 gtatcgaagc aaaagcatat aaattcccat tttcagggaa cggtcgggca ttatcgcttg
    16501 gtaaaacaga aggattcatc cgtctagtta cgacgatcga agataacgtg cttattggcg
    16561 cgcaaatcgg tggagtcggc gcaagtgata tggtctctga attagcttta gcaatcgaat
    16621 caggaatgaa tgctgaagat atcgccttga cgattcatcc tcatccatct cttggcgaaa
    16681 tcgtcatgga tgcttcggaa ttagcattag gtttaccaat ccatatttga tttgaataat
    16741 acaggcgaaa cgcaactgaa a
//
LOCUS       DADXTH010000067        16614 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.067, whole genome shotgun sequence.
ACCESSION   DADXTH010000067 DADXTH010000000
VERSION     DADXTH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.067"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(247..912)
                     /locus_tag="IWI97_002099"
     CDS             complement(247..912)
                     /locus_tag="IWI97_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463593.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1092..3074
                     /locus_tag="IWI97_002100"
     CDS             1092..3074
                     /locus_tag="IWI97_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBC2463594.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3078..3716
                     /locus_tag="IWI97_002101"
     CDS             3078..3716
                     /locus_tag="IWI97_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463595.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            3744..4172
                     /locus_tag="IWI97_002102"
     CDS             3744..4172
                     /locus_tag="IWI97_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463596.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            4629..5540
                     /locus_tag="IWI97_002103"
     CDS             4629..5540
                     /locus_tag="IWI97_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBC2463597.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            5757..6194
                     /locus_tag="IWI97_002104"
     CDS             5757..6194
                     /locus_tag="IWI97_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463598.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            6289..7173
                     /locus_tag="IWI97_002105"
     CDS             6289..7173
                     /locus_tag="IWI97_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBC2463599.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            7397..10537
                     /locus_tag="IWI97_002106"
     CDS             7397..10537
                     /locus_tag="IWI97_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463600.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            10584..11129
                     /locus_tag="IWI97_002107"
     CDS             10584..11129
                     /locus_tag="IWI97_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBC2463601.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            11157..11612
                     /locus_tag="IWI97_002108"
     CDS             11157..11612
                     /locus_tag="IWI97_002108"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBC2463602.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            11609..12367
                     /locus_tag="IWI97_002109"
     CDS             11609..12367
                     /locus_tag="IWI97_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBC2463603.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(12453..13193)
                     /locus_tag="IWI97_002110"
     CDS             complement(12453..13193)
                     /locus_tag="IWI97_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2463604.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            13254..14507
                     /locus_tag="IWI97_002111"
     CDS             13254..14507
                     /locus_tag="IWI97_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBC2463605.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            14829..15056
                     /locus_tag="IWI97_002112"
     CDS             14829..15056
                     /locus_tag="IWI97_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBC2463606.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            15179..15505
                     /locus_tag="IWI97_002113"
     CDS             15179..15505
                     /locus_tag="IWI97_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBC2463607.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            15573..16286
                     /locus_tag="IWI97_002114"
     CDS             15573..16286
                     /locus_tag="IWI97_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBC2463608.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         5683 a         2694 c         3338 g         4899 t
ORIGIN      
        1 gtcgttaatc tattcggatc gaatgagcaa aatatttttg tgaatgtcca tccttgatta
       61 tgattccaaa aagaatcatt ggaaggctgt ggcgtaaata ttatactata agccagaaat
      121 acctatcatt ctgaactttt ttatttagga actttcatct caaggctttt tcaattaatt
      181 tgtacccctg ttttttggat aagatcatgt gcattgattt tttctatctc tacgttcatc
      241 tcctacttaa tgcctatctg gctcctgagg gcttcttttt aaggcgtcgt catagtcttg
      301 gctgaggatt tcttggtaat ctgagggaat ggcttcccaa gtgagcaggc gaacgttcat
      361 gacgtgtttt cctttgtgtt tgattacagc atggtttttc tctttttcta cctctccagc
      421 gtgaaaagac agccatgtac catggccaaa gatatcatta catacccaac gttcgatgga
      481 attgtccacg tatcctacag ggagtgtggt atagccgatt tgtggtttca aaaaaggatt
      541 gaatcgatcg atcgtttcat ttttgtattg ataattgata agaaaaagat agaccaaaca
      601 aaagacgagc agaaagaaag atagtgtctt tttgagtgag aagggttgtt ttgatgaata
      661 agatttcttg ttaacaagag gttcatctgg caatgttggt tctattgatt cggtagctga
      721 ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga tgtaaaagtg acagcatttc
      781 aaagtctggc attgtacgag aattttccca actagaaatc gttttatcgg aaacaccaat
      841 tttttcgcct aattcttttt gcgtaagctt acaatttctg cggtaagttc gtattttttc
      901 tccggtgttc atcttcttct cctttcgatt ctacagtata tagatttatt ctacatgacc
      961 tatacacttg atagatacgt ttttttgatg aaaaacggta tgattttatc aaagaatcat
     1021 ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac agttttactc acaatcatag
     1081 tgaccggtat catgagtggt tttgccttgg ttagtataaa taaacaggta ctctatcgga
     1141 caaatgaatt tttccatcaa acagatactt atactccgat tcgattgcca caacatttta
     1201 ttgggcaaga agaatatgag cagctaatca tggtatttga aaatgtctca tcagaaactg
     1261 agctttctta tatgaaaaaa acagtaaatg aatccaatga agtagataca tcttggaact
     1321 tcaagataca gccaaagctt gaaatgacat tgtattatca aggggatgtc aaatcttctc
     1381 acgagaaggt accctattct aatacctcct ttcgcgtaac gtccattgaa gaatcgatca
     1441 aaataaaaga ttttgaaggt gaatttttct tgatgacgag agaaaaagaa gcctatgaac
     1501 gggtcatttc actattcact gatcgattca atcaagcatt tcacactgat tatgccacta
     1561 atatttttga agactttgaa aatagctatt cgtattcggt tatttcaaat acagacgtat
     1621 ataattatcg agtgtattta actattggat tgattctttt attccttttg ttatcatcct
     1681 ttttcttatt gatgaataaa gaaatcagtt tattgttgaa taatgacttt tcattacatc
     1741 aagtattgtg gtgtttattg gggaaaaaga aaatcattat tacattcata attgcaggaa
     1801 tcattggtgg ttttattttt atgtattata aggagcaatt tctttatttg ttccagatgt
     1861 acgttttctt gttcagcgga atatatggta tctctttgat ttctctttta ttagttgaaa
     1921 aaatcgcttc gttaagagaa gcgttcaact atatccaata cattcttttt acatttggat
     1981 tgttgttttc tcttcttttg accgttgcca atgtcgaatt agcaactatc gttttttctt
     2041 cttcacagtt agctgtttcc catgaaccac caaaagaaat cgctggtgag gactatcatg
     2101 tattttatcc agtaaccatc gggaaaaacc acgtggattt tatttattcc aatcgttttg
     2161 catcagcagc agatcaggaa ttatatgaaa ctttgaataa aaacggaagt atattagtca
     2221 atgtatcaga ttacttgaat gaagaaaatg aacagtttgg cagagggata aaaataaatc
     2281 tgaattatct taagaaattt cctttgattg atgtctctgg aaggttgatc caaataaccg
     2341 aaaaagaaac gcaccctgtt atattgatcc ctgaacgata tagacataca gatttaaaaa
     2401 atgacctggc gcaaataaca gaatattatc aagaaatcag tgaaaaagaa ccgaaatttc
     2461 tatttataaa aaacaatcaa cccatttata catttattcc ttctatcccc tggatcgaac
     2521 attatccggt tgttgaaata ctgacattga aaaacagtat gtatttggaa agaaatattc
     2581 tcagcggaga aatctatcca ccgttaaaga tcaagatagg ctctttgtcg aaagaaaggt
     2641 tatttgagct gattgaagaa agtcagttac gtgataactt gcagttatca tttccgtata
     2701 ctcagactga agagatagct gtcaaagtgt taagcagatc atttcattac ttgatacaga
     2761 tatttttact cactttcttt tctttcttct tgttgagcta tgtcatgtta tgcatatatt
     2821 ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag tggttattcg ctctttgaaa
     2881 cgtataaaga tttcttcatg atgaacctca tcaaatgggg aacgacaagt gtaatcttcc
     2941 tttttcttat agaaagagag cctaaatatc tttttaatat ttttttcttt agcttcattg
     3001 attttattct ttcagttgtt tttattttgt ctacagaaaa gaaaagtcag ttattgctga
     3061 tgaatggagg ttaacaattg gagccgttga tagtgataaa aggtgcgaaa aaaatcattc
     3121 gaaacaaaac gattcttcat ttggatcgtt tcactgcgga tgctggggat cttgtgactg
     3181 ttgttggaga aagtggagca ggtaaaagta cgttattgaa tatattagga ttgaatgatc
     3241 agttcagttc aggacaattc tatttgttcg atcaagagac gaaacaatta actgggagag
     3301 agaagcgctt gataaaaaga aataccatat cttttctttt tcaagatttt gggttgattg
     3361 aatatgaaac aatcgatttt aatttagata ttggattgaa gttttcgaat ttaaatgttt
     3421 cagataagcg aacagcaaag tatgaggcat tgaaaaaagt aaatataaac aagaagctaa
     3481 gtacgcctat ctacacatta tctggtggtg agaagcaaag aatcgcctgt gcgagagtct
     3541 tattgaagaa tagcaaaatc attttagccg atgaaccgac tgggtcattg gataaaagaa
     3601 ataaggataa agtagttgaa atcttatcgc aacaagcttc ccaaggtacg ttagttatcg
     3661 tagtcacgca tgatccatat ctgaccaaaa taggaaacaa gaagatcatg ctttaaaaat
     3721 caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat tatttgtgca ctagggatag
     3781 gttttgttgg aggaaacgct atgtcatctg aagtggaagc aaattcagtt cacggtgaaa
     3841 tctattacaa atctttaaag ccaggagaga ttcattttga tgctaaaggt aatattatta
     3901 agtcaaattt accagtagac cgggaaattt ctagaacagc aattaatcat agctctggaa
     3961 gatgggttta ttatagtgat acaacttgta cgagaaaaac aggtcattct aaccactata
     4021 gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg aaactctatt cgatcagcaa
     4081 atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg aaatagaact gacacattct
     4141 catgttggta caatccaacc ggttggtatt aagtatccaa aagtttatat aaaatagaaa
     4201 aaacatataa tgaaacagcg ttaaaagtta tttttggtag ttttaatata ttcattttta
     4261 gttgtataaa aatatatcat aataaaaaca acaaaaaaat gtctggaaaa tcgaacataa
     4321 aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa acaaaatcga taacatttgt
     4381 tctaagaatt agttacgtta aatacggaac gcaagataga cttgaaaaac ggtaacaaaa
     4441 gaagtagtat attaaggtgt gatgtgctac ttgacattta ttctgctgag cgaactttct
     4501 ggattgtgta gccttatatc tattcaggac gtatcgagca gattagtaaa ggtgttcttg
     4561 atgattttca tcttgaacac ctttgttttt agcaattcgc aaaaaacgaa aagtaggaga
     4621 attgataaat gaaaaaaaga tttttagcac ttgccatcgt actaggcact ggcttattgt
     4681 caggctgtac gaatgccggt gaaaaaacag ccgttagtta taaaggcggt acaatcagcg
     4741 aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc agattccgct gtgcaacagt
     4801 tgatcgttta tcaagtattt gaagataaat atggagatga cgtatcaacc aaagaaatcg
     4861 attctcaata tgatcaaaca aagaaacaat tgggtgattc atttgattca caattgaaat
     4921 ctgcaggtta cacagaacaa acattcaaag acagtatcaa acaaagtcta gcctttcaag
     4981 aaggtctaaa gaaacatatc aaattgaccg atgaagattt aaaaacggct tgggaatctt
     5041 tccatccaga agtggaagct caaattatcc aagtagcaag tgaagatgat gcaaaagatg
     5101 tgaagaaagc tgctgataag ggagacgatt tctctaaatt agccaaagac aagtcaactg
     5161 atactacgac aaaagaagat ggcggaaaag tgaaatttga ttccactact acaacggttc
     5221 cggcagaagt caaagaagca gcctttaagt tgaaagatgg tcaagtttct gatgtgatca
     5281 cttctaccaa tgcttcaact tatactacgg aatattatgt ggttaaaatg gtgaaaaacc
     5341 aaaacaaagg caacgacatg gataaataca aaaaagaatt gaaagaaatt gcaacagaca
     5401 caaaattaag tgacagtacc ttccagaata aagtaattgg cgaagtattg aaagacgcta
     5461 atgtaaaaat caaagacaaa gactttgaaa atgttttgag tacattcact tcagattcta
     5521 gtacagcaag tagtaaataa gcggtataaa taaaaaatat gaactattta aatcaataaa
     5581 actgttcatc taaatctttt tattagaact aaatcaacag tagtttaatt actacttgat
     5641 ttagttcttt ttattttaaa aatttttttt acttagaatc cagcgaccct agatattttt
     5701 ctgtataatc ttaaataaag aaaacaatat caaaaaatgc acgtagaagt agataaatgg
     5761 agtggttttg caaatttctt ttatttctag aagttattca cgtatacgga tttgtttttc
     5821 tcacaaataa aaaggcagga agtacaatgg atcatttgta tataaaatat attcatcaat
     5881 tttccgtttt ggtgttaggc acactaatta tagcggggat tgtcgcattt attattcaag
     5941 gccgtgacat attaacagta catcaagagt ttttcagtta tgcatttggt attcctctga
     6001 tcgctataag gtctgataac aaaaagtgga agaaacgcta ttacattctc ttgctatttt
     6061 tgtgttttta tctgccacct aagattttta ctttattagg tatacaatgc ctatcatttt
     6121 cttttatttc ctatatcatt gttttaattt tcctcagttt catagtatgg atgttttatc
     6181 ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca tcacagccta atttttttct
     6241 ttatgctatc gttatattaa agaaatcaat cagaagggaa agaaagtgat gaaaaaagtt
     6301 ttattagtgg taaaccccag ttcaggcggt gaacaagcaa aggaatttga gcaattagcg
     6361 atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct tgcatacaaa aaaagcaggg
     6421 gatgcaaaaa actttactcg cgaagcggct acggaagggt atcatagtgt gtttgtcatg
     6481 ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg ctgaacaaga acatcggcca
     6541 aatttcggat ttttcccatt gggtacggtg aatgacttgg ctcgagcatt agggataccg
     6601 ctagaacctg aggaagcgat caatcatttt tcgattgaat cagtcaagcc attggatatt
     6661 ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa ttggctctat ccctgaatca
     6721 atcaatgatg tagatgcgga gaagaagacg aaatttggaa aactagctta tttcatgtca
     6781 ggaatcaaac agttagcttc cacacaatcc tactcgtttc acgtagaagt tgatggaaag
     6841 aaggaagaaa ttgagagcag taccttgctg ataggactaa ctaactcagt cggcggtttt
     6901 gaaacattac ttccaaatgc aaaagtggat gatggtaagc tccatttggt ttatttaaaa
     6961 gattcttctt tgatcgatac gcttaaaaca ttgccagacc tattaaaagg agtagatgaa
     7021 tctacgaaaa atctagccta ccttacttgt gagcaattag ttgtgtcatt agctgatgag
     7081 gaagaattga cgacgaatgt tgatggagat gaaggggata aattacctgt gacaatcaaa
     7141 gtattgccct cacatttaca ggtttattgt taaaatggag gttgtgaccg aggagttggc
     7201 gggagaacac cgcttgttcg ggataaggga ctgtgacaag acttttgtca cagccttttt
     7261 tttgtacaaa aaagaaattt acacctaata gaaaaatttt tccataaaaa tacatatttt
     7321 aaatatgaaa gtacaattgg aaatagatta aaacgataaa ataaaattgt caaaacgtca
     7381 gagaggatgg tggagaatga atcaatgtgt aaaaaagctg attgcgtcga ttttgtttgt
     7441 cttccctctc ttgatggtga catccgtttt tgccgatgta gagaaaacaa acgaatcaac
     7501 gcaatcagag aatcaaagtt ttgacaagac tactttttca gaagaatcga ttacttctga
     7561 aactacagaa tctcaaagtg aggcttctac taataaaagg caagaagaaa ataagacaag
     7621 tgagacctca gaagtagctg aacaaacgtc tgatagcagt gaagcggcac tggcaagagc
     7681 tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa gaactggcgc agcttctcgc
     7741 agacgaatca gtaactaaga tccgactgat tcagcctttg actttagaca gggaattaga
     7801 gatcaaacga gatatcgtta ttgattttgg tggattcgct cacaatttcg gcacacacca
     7861 tatttatatc aatgagatac ctcagtcgat cgagttccag aattttaaag gaacagctgc
     7921 acatccaggt ggattgacac caaatatcga tggaaatgcc attatcattg cttatatgac
     7981 cgatttttgg ggaagcagat atcattttac gggagaaata aaatttaccg ggacacttga
     8041 tttgtatgat gggtccaaat taggactgat ttacgctcct cgtgcaactg tcacattaga
     8101 tggtgtttcc ggtgtgttgg atgtccaacc agtgaaggaa ggactgaatg ccgctccggg
     8161 taaagcctat ttttgtagaa gctatcaatt gaatgtgatc aatggtagtc aactttatgg
     8221 accgtatcta gggaaattct acggttttct agattcagga gatgagactg gtagtggaaa
     8281 aacagcgcca ggaatccata tcttaagcgg ttcaaaggtc tctcttgatt atgatcgggc
     8341 agatggcgtt agcgatgaag gagaggcagt cgatacgcta cctggtaatg tcatgtttga
     8401 agtcagtggt tcgggaagcg aattttcggt aaatacaaag atcaatatta ccaatgacaa
     8461 caatcgcgga atcatccaaa tgagaggttc aggatcgaaa gtcactgtat cgaatggtgg
     8521 gaaaatcacc atcaatacag caactacggg cggatttcgc ttgcaaggag attctagtca
     8581 aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg acgatcaacc
     8641 cgggaacaat gggatgcgtt ttgtaggaaa aggcctttct ttgatcgtag aaggagccgg
     8701 atcagttatc gacatcgata agcagagtgg gcgttccagc gcagttcgtt ttgagaatgg
     8761 tacgcaaaag ctgacagtaa ccaatggagg ctcattgagg gtaagaaata caggtgatgg
     8821 caaatcttac gtgaacagac tcaatcaagg aaatcaagcg atccaatttt atgacgcttc
     8881 tggttttcta gaaagtccag gttcagctga ttttacagtc actggagaaa aatcaaatat
     8941 cgatatacgc gcagattccg gagcaactgt agatacaact ggagatattg atttaaattt
     9001 tacggcaggt gaaaaaactt atttagtcat ggtcgggaaa acaggggaag ataagaccgg
     9061 catcatcagt ggggatgtgt tgaatgtgga cttagaatct cctacctatt ttgatttcca
     9121 aaacaaacgg attggaagcg agaccaatgg cataggcggc tgggtattcc aaggaaatgg
     9181 agattcttct ttaaagatcg cccattcaga aatcgcttta tggcgaaaaa caggcgcaac
     9241 attcgataac tttgaaaaag acccggatta tttttctgat atgacagact tatcgatcaa
     9301 tgggaccgac ttaggcaatt tcgtatcaag ccctagtgca ggtttaacaa atgaatttgc
     9361 tcagacaggt gccggaatga aaaattataa tcgaatcagt gccaataatc agctcccgat
     9421 gattgaatcc ttaagagtgc caacgaatgc agataaaaaa atctatgggc atgtcgttgt
     9481 tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag aagtgacttt
     9541 acgtattact tacgcagata cgacgaaatc atctgtagaa attactgctc tcacgcaagg
     9601 acaaacagat gaaaatgaag gggcaggata taacccatgg ggagaaggcg aacaaggcgg
     9661 cttgttcgaa gttcaagtac ccaatgatga gagattaaaa acggaggatc aagtgcaagt
     9721 tgttgctgct aaaaaagtaa gaggtggcca agaagtgaca gaactggact ctgccaaaac
     9781 gacagtcgat gtgattcctc ctgaacctgc taatttaagt accacattga tcaatgcagc
     9841 ttctcgcacg atcacaggta caggtgaagc aggagccttg gtttcattga aaaaaaatca
     9901 agctgttttg tcagaaacaa ctattgatga tacaggggaa tttgttcttt ctatcccaga
     9961 aaatcaactt tttgcaggag atgtattaga aattgtatta aacgaccaag caggagaagc
    10021 tactgctaag ggtgtgcaga acaagccaag taccaacgat gaaattggga atcacaatcc
    10081 aagtgatacg ccagttgttt atcatgatgc cccgcctttt gcacctgcta cgaagatcgt
    10141 agtagaaggt ggtctctcat ttcttgcccc ttccaaactc gattttggtc agatcaaagc
    10201 aactggtttg acacaagaag catttggcca aatggatata caggaaaaat tatggattta
    10261 tgatcagcga gaaacgaaaa atccgtggat cttaacgata aaattatctc agcctttcac
    10321 aaatgatgca ggattttcta tgcaggatgt cctatatttc aagaaaggag aggagtatca
    10381 attagtgaat gaagaagaac tggaaattgt cagaggacaa aattcaacga atgtgcaagc
    10441 agattattca gaatatttaa ataacggtcg ggcgtttaaa cttgtcttaa ccaaagaaaa
    10501 tcagatagct ggtaattact cagcagagat aacatagaca ttagcagatg cgccgacaga
    10561 ataaagatca aggaggatga ttgatgaatc gaatatggaa taaaagtcat cgaaacaact
    10621 gctctttact tatgatcctt ctccttggat tcatcctttg gcagacacct gtccatgcct
    10681 ccgaattcaa ttttgcggta gattttgaat taccggctaa tcagattgat aagaacaaaa
    10741 catatataaa tgtaaggatg gagcctggga aaacacagga aatcccctac acggtcttta
    10801 accgaacaga taaatcagta acagttgaga cattgatcca ctccgctacg acgaattcaa
    10861 acggtgtgat cgaatatgga gaatcaaaaa gtaaatttga tgaaacatta agttatgata
    10921 tgaaaaactt agcaaaaaca gtgggagaaa taactgttcc tcctcaaagt gcggtcactg
    10981 agacactttc actgaaagcg ccagaaaaag agctggctgg tgtggtcgca ggagggatca
    11041 cgtttcaaga aaaaaaagag aacgtttcta acaaaagtac agttggtgtc gtcaatcagt
    11101 tttctcaagt gaaagcaatc ttgatttgaa atagcgaaaa agagtcttcc ccagatttga
    11161 aattaaaaaa agcttatgcg gatcaaagcg atggaagaaa tgtgattaag gcgaatcttc
    11221 aaaatccaaa tgcttcttat atgttcggct tgactacatc ggttgaaatc tacaaaaaag
    11281 aaacaaaaga gccttacctt tcaataaaaa aagaaaaaat agatgtagca cccaatacaa
    11341 attttgattt acttgtccca ttagaaggaa aaaaattagc tccgggacat tatcaagcgg
    11401 ttgtgaaggg tgcttggaaa gaaaaacgct ggaatctcca taccgatttt gaaataactg
    11461 atgatgcagc ggaccaattg aatgaaaagg atattgattt aaaaaatcag ccagaaacat
    11521 ttgactggcg atggctgata cttgttggag tcattcttat tggcttattt gctggattat
    11581 ttattttaaa agaaagaagg aaaaagaaat gaaaaaaaca attgcattaa gtaccgttct
    11641 agctggaagt ctattattat gtacacaaag tgtttttgct gagaataaga gctcagccac
    11701 tacagaagga acgatttctg tgaccaaacc agatgctgca aatcctgaag acgttgttcc
    11761 agttcctcct gttgatccaa atgatcctga tactcctttt acacctgacg aaccaaatca
    11821 gggaacatcc ggattactga cgattgacca agctccagat cttgattttg gaacaatcaa
    11881 gttaggtaca gcaaaaacag tatatgctgc attacaaagc ggaacggaca gtactggagc
    11941 acaaacacaa gtacagaatt tcgtacaagt aacggataaa agcggaaatt atgccggttg
    12001 gaaattatca gtcaaacgaa cagaatttat tggtacaaca tctgatacga ataaactaac
    12061 tggttcaaag gtgaaattca aaaacgccgt gattcgaaat agcgcaacta atagcagtgg
    12121 aatgccatct accgtttctg gaactggcgc tgaaggaatc gaaatcccag cagatcaggc
    12181 agtagatcta gtaactgcag gagcaaatga agggattggc acatggattt attcattagg
    12241 agagaatgtg gaacaaggga gacaatcagt ggaattattt gttcctgtat ccaattatgc
    12301 tgtagacaac tacgtttcaa cattcacatg gtcgttaaca gatgctccta cagaagagga
    12361 agcgtaaaat catatcaacg acttcaggga gtggagccga ttcaaggccc actccctgaa
    12421 atctgctatg cttacgtctc agctcttatt catcaactcg aagcagtttt ttggaagtca
    12481 tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa cgagtaagga gtcaccgttg
    12541 aaaaattcta acacataatg agcagaatca attttctgca attgcgataa gttgatacag
    12601 taagatttta aaagtaaaaa gtaagcatac ttttctaact ctgtcttcaa ttgttttaaa
    12661 gaagtattca ctaatttatc ttctgtagtt gttttgacca taacttggtt tttcaaacct
    12721 ttgatacttt caatatataa aatttcccga ggatacagga cgagacttcc acctttttga
    12781 tcaataaaga ttttatcgac atttacgatc tgctgttctt tccaattgat ctgttctagc
    12841 acttgacgaa tttgttcaga taaattcctg tcttttctga tatatccaat cggaaggatt
    12901 ttccgattga tgatctctac tgcaaaattc gtataagcgg tgaaaaaaac aatattacta
    12961 tgtgcatttt ttttacgtat ttgttcggcg acatcaatac ctgtgagtga ttgcttcaag
    13021 tcgatatcga taaagaaaac atcgtgatta tcaatagtgc ccaactggat caactgattg
    13081 atttgagaga tagaagtgag ctgcacgata ttctcgatat gatgattgac caattctctg
    13141 atgatccatt cgctgtgaaa ctggttatct tctatgataa aatacttttt catatgcagg
    13201 ctcctattaa aaaaatatgt gtacattttg aaacaaaaca atggaggaat aagatggtcg
    13261 caacagtgac actttatttg acctttactt tgattttaga tttaggtatc tatttattct
    13321 tttcgaaaca actgctagta aaaaacagct ttggttgtat agctttgatg gtggtgacac
    13381 ttttacttcc tatccctaaa gtgagctttc cttacctaat tggcttatta ttgatgtttc
    13441 ttttttattt tactacgaaa aacctgttta tcagctgttt gatcagtaat cttttttttg
    13501 ctatcactgg actcagcttt tttctggttt ttgatctgat ggattattta ggaaagagac
    13561 atcttggaac agcaagtttt tttgcactac ttatcgcatt tattttctat ctattagtaa
    13621 aaagtataga caaacagacg agatttctca acttgctttt tacttatcca actgaacact
    13681 attttggagg tatttgtatt ttattaagct ggggcgtgat ttacttggta ttgggaagtg
    13741 ttcacttccg ctcatctaat tatctgcttc tatctctttt gtcgatagtc atcagttttt
    13801 tttctttgtt tttttccatt atgttgatca gtttccaagt aaaagaaaga caacatcagg
    13861 aatcgcttca actgtatcaa ataaatgaag aatattacca tcatctagag gaattcaaac
    13921 atgattataa aagtctactg ttttcattaa aaattactct tcaggaaaat aaaaaagaaa
    13981 cgttggaatt tttagaaata ttagaagatc attctgcagc tatttttgaa aaaccgcaaa
    14041 tacaacagct aacaaatatt gtatccccag ctgtcagggg tgtcttttta aaatttctag
    14101 aaaaagcaga atcggagaaa atcccgatcc aagtagttat tcctgtcgaa gtgaaaacaa
    14161 tcccgattga tctggttgtt tttattcgct gtttatctat ttttatttct aacgcattcg
    14221 atcatcgtgt agccgatcag agtccgatta cgatcaatct gctggaagaa aaagagggat
    14281 cacgttttag tatagcgaac aaggcaaatc caacgaattt gacgctatcg gagatggtca
    14341 aaagaggaaa aacctctaaa aaaaatggcg ggttaggtct atacagtgcg aaaaaaatgt
    14401 tggatgttta cgaaaatgca gaactaaaaa tcgaattttc aaaacagaat cactattttt
    14461 ttgtagaaat gtatctggca tctagaaaga gcaaatcagc ctactgactt actgtcaaag
    14521 gataagtttg aggaaagtct acacggagat agagacaaca acacagcata ttcaaaagaa
    14581 acagaagcgt gatgaccgtt gtcacccttc tgtttctttt gctatgtagt taaacaaagt
    14641 agttgacact tccgctactt agtattagta cgaaagtata cttcatgata ctaagtagat
    14701 aagaagtgaa aagcggaacc cacgatcgtc aaaatgctaa ctgctttgct tacaccagat
    14761 aaagggaaga gcaatatcgc ttattttagg cgtggagaag caagcaaatc cctacgatca
    14821 caaaaaaaat gctgacccag cagttaagag agttagaaca agatgaaagt atccatcgaa
    14881 aagcctataa ccaagtcccg cctaaagtag agtactcgct caccgaagaa ggaaaatcat
    14941 taagagagat tctagtagcg ttgtctgttt gggaagaaga acgtatcgaa aaacaaaaag
    15001 cctcaaggga agaagacacg ctactgcata cacatggtgg ctacctaaat tattgatttt
    15061 ctatgacatt tattttaaat aaaagaaaac aggagttttt ttaaaagcat catctatttt
    15121 ttatgtaaaa aaatggtatg attaatcaag taagaagtga ttggaagaag gcaagcaaat
    15181 gaatagtatg gaaaaagtca accagtttag agatgaacgt aattggcgac aatttcataa
    15241 cgaaaaagat ttggctatct ctatttcttt ggaagcatct gaattgttag aactttttca
    15301 atggaaaacg ccggaagaag taaaagaaac acaattagaa cgaatcaaag aagaactggc
    15361 agatgtattg atctattcat acatgatggc agacaacctt cacctggatc tagatgagat
    15421 cattgaagaa aaattgatca aaaataattt gaaatatcct gtagaaaaaa gcgcaggtgt
    15481 acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg ctgttggaaa gagcaaaaga
    15541 aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa aaaaggaaaa gcaaaaagat
    15601 ggctagtcaa tatcctcttg ttcctcttgt tattagtagg actagctttg attttcaatg
    15661 aacagatcaa ggattatttt gtcagagaga caggagataa atatgctata gcgaatgtaa
    15721 caaaagaaga tctgaaaaag aataacgata aagatgtcag ttttgacttt gatgcggtag
    15781 aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag aggaacagat ctaccagtga
    15841 ttgctagtat cgcagtccct tcagtttcaa tcaatttacc ggttttcaaa ggcttggata
    15901 atacgtcact actttacgga gcaggaacat tatctccaga tcaagaaatg ggaaaaggaa
    15961 attatgcctt agcaagccat cgtgcaacga accctgaatt gctgtttaca ccgctagaaa
    16021 atttagagat gggtgccaag atctatttga cagatttaga aaatgtgtat acatacaaaa
    16081 cattttttaa agaaaaagtt gctcctacag atacccaatt actgaatgaa gtcgagggaa
    16141 aagaaattgt cacactaatt acttgtggcg acatggacgc cgtcacgcga ctagttgtac
    16201 aaggagaact ggaaagtgtt acatcgataa aagatgcgac agatgatatg agatctgctt
    16261 tcaatctaga gacaaaaaca ttttaggata ctgggacata agtcgttaca gattaaaaac
    16321 agccaaagga tatgaatcga taaggaaaaa ccaatttgct gaaaaaaaga gaaacgcaaa
    16381 gaggtgcatc tctgatttgg ttgttttcta gatagacttg tgttccagtt tttttatttt
    16441 ttatatgaaa aggtttgcat aaataaaaat tcgatggtat gatgattttg aataagaaat
    16501 ggattgttac tgtacggcag gcaaaaccag aattcagcta agaaacagta ggcgaattct
    16561 aggttttttt atttttcttg agagggcgaa gcaaaatgaa aattgataaa attt
//
LOCUS       DADXTH010000068        15975 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.068, whole genome shotgun sequence.
ACCESSION   DADXTH010000068 DADXTH010000000
VERSION     DADXTH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.068"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(253..2634)
                     /locus_tag="IWI97_002115"
     CDS             complement(253..2634)
                     /locus_tag="IWI97_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBC2463609.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2624..3271)
                     /gene="recU"
                     /locus_tag="IWI97_002116"
     CDS             complement(2624..3271)
                     /gene="recU"
                     /locus_tag="IWI97_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBC2463610.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3335..3874
                     /locus_tag="IWI97_002117"
     CDS             3335..3874
                     /locus_tag="IWI97_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBC2463611.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3964..4383
                     /gene="gpsB"
                     /locus_tag="IWI97_002118"
     CDS             3964..4383
                     /gene="gpsB"
                     /locus_tag="IWI97_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBC2463612.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4405..4772
                     /gene="rnpB"
                     /locus_tag="IWI97_002119"
     ncRNA           4405..4772
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWI97_002119"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4981..6147
                     /locus_tag="IWI97_002120"
     CDS             4981..6147
                     /locus_tag="IWI97_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBC2463613.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6193..7689
                     /locus_tag="IWI97_002121"
     CDS             6193..7689
                     /locus_tag="IWI97_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBC2463614.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7770..8453
                     /locus_tag="IWI97_002122"
     CDS             7770..8453
                     /locus_tag="IWI97_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2463615.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9098..10288
                     /locus_tag="IWI97_002123"
     CDS             9098..10288
                     /locus_tag="IWI97_002123"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBC2463616.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10390..11343
                     /locus_tag="IWI97_002124"
     CDS             10390..11343
                     /locus_tag="IWI97_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBC2463617.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11412..12761
                     /locus_tag="IWI97_002125"
     CDS             11412..12761
                     /locus_tag="IWI97_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2463618.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12944..13267)
                     /locus_tag="IWI97_002126"
     CDS             complement(12944..13267)
                     /locus_tag="IWI97_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463619.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13396..14559
                     /locus_tag="IWI97_002127"
     CDS             13396..14559
                     /locus_tag="IWI97_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBC2463620.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14629..>15975
                     /locus_tag="IWI97_002128"
     CDS             14629..>15975
                     /locus_tag="IWI97_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBC2463621.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
BASE COUNT         5103 a         2810 c         3388 g         4674 t
ORIGIN      
        1 gtggcactaa ataaaagact agtgtgtatt tagccgattt aattataaac aagtaaatgg
       61 ataaagtata tgtaatgata aaagaaaaat ggattttcta atttttaagc taaatgttag
      121 atcacacatg tatatgctgg atagtcttat aaaaaatcat gtaatacagc ttggtacatg
      181 atttttattt gtagctgttt tttacataga cacaaaaaag aaaccacgct tgtagacgtg
      241 gtttcttttt tactatctgt ttccagaagc tgaggtgctg ctggatggtg tatggtcatt
      301 tgcagcatcc gctccgctag ttgctggctc actggaagaa gcaggaggag cagatgaaga
      361 cggtgtcgag ctttcttcgg atgaagaaga aggaaccgtt gttgaagacg ctggctcgct
      421 tgtttctgct gtcgtgcttt cttttgagga ctcagcagtt gattcgcttt gactagtaga
      481 gctagagctt tgagtcgttg tctctgttgt tgagcttcca ggagtctgga catagctgga
      541 agatggaata gttgttgatg gtgtaattgc attcgatcta gctgtatatt gatctttgta
      601 gtacagttca ccgcccacac gaattaatcc gcttggcatc tcccagtctg tatttgtcac
      661 gttagctgaa acatactgca taagctcgcg ataaacatca gaggctacgt gtgacgattc
      721 tgaagtgaca ggtgtcattt ttttattgta acctgtccac acggaaatcg aataatttgg
      781 cgtatagccc gcaaatagga tatctggata caccccagaa gaaataccaa gttttgcata
      841 ctcgtcatct gtgtaattgg atgttccggt cttaccggct tgatataacc cagcgatttg
      901 cgcattcgta ccagttcctt cagtgatcgt atcttttaaa atgtctgtga tcatataagc
      961 tgtttctgga gacatggctg tttttccatc tggctcataa gtttcttctg ttccgtcttg
     1021 gaagacgatt ttattaacat attgaggttt gtaataagtc cctccattgg cgaaagccgc
     1081 ataggctgct gccattttta aggaagaagc tccgtatttt gtcccatctt gttcttccgt
     1141 attactagag atcgcattcg attgatggat cgtactgtat tcgatcccaa gattttttaa
     1201 gaaagaagcc actttatctg aaccgacttc attgaataat ttgactgctg gaacattacg
     1261 ggataagtaa agtgcttggc gtaaagtaat ggtgcccata tattgattat cccagtttcc
     1321 tacaggagtc gaagtccctt catagttata cggtgcgtca gtaatagttt ttccagtaga
     1381 atatttcaaa tattcaaaag ctggtccgta atctgtgaca ggtttcatcg tagaaccaaa
     1441 atcacgggaa gtattcactg ctaagttgtt gcctaaagtt acgtcttctg cgatgtgacg
     1501 tccgccaatc tgggctttta ctttcccggt attggtgtcg ataagggtgg aggcaacttg
     1561 catctcgtcg tctgggtaag agacatactg atccgtgttg acgatatcat atagtttctt
     1621 ttgggcgtcc agatccaaat tcgtataaat ctctaaaccg tctgtgtaga cattcttatc
     1681 tgttttttct tgtacttcat tgatgacttc tttgacataa ttatcgacga tcttagtatt
     1741 gtcatcggat tgagttagtt cttgcagacc atccgttacc ggtacattaa cagcttgatc
     1801 atattctgtt tgagagattt tttcgttttg aagcatcgta tacaaaacag tatctcgtct
     1861 tttcttcgcc tgatctggat aaacataagg atcataggcg gaaggggctt gaggcatccc
     1921 agcaagcaat gctgtttgcg gtaaagaaag ttctgataat ttcttaccaa agtacatctc
     1981 agaagctgtc tccatcccgt acaagccgtt tgacatatac actttattta cgtagtaggt
     2041 caagatctct tgtttcgatt ttttttgttc caatcttaca gccatccacg cttcttgggc
     2101 cttgcgtttc agagtctgat cctcggcact ggtggagaag aaggaaagtt tgatcaattg
     2161 ctgtgtcaaa gtacttccgc cttgcagtcc gccagaggta aagttagaga gggcagaacc
     2221 gatgatccga atagggtcaa ccccgatgtg tttataaaaa cggcgatctt ctaccgacac
     2281 gatcgcatct tctaatgttt tcggtaattc attcgcagaa attttttcac gcttttctgc
     2341 gcctaaatct tcgaatagtt cgccatcttg tgtatatagt ttggaagata cggtagcgtc
     2401 taatttttta tccgtcaatt cgggcgcatc ttttgcgtaa taccagaaaa gtcctacacc
     2461 tgctaaaaag aggatacaga aaaatgagag tattcctaaa aggatcttga taaataatcc
     2521 ttttttatgt gttccagagg actttccaga acctttgcca gaattcttct tcacactctt
     2581 tttaggagta ggctgtttct gtcgtctaga gcttctagtt tgttcatttg ccattcttgc
     2641 tttctccttc aatagataat gttgataaat agaagtcaac cgcgtccaaa taaggaatcc
     2701 gcggagaaat accagttttg atctctattc ctagttcttc gatcaaagat aaaggtagtg
     2761 atttgcgtcc tttttccttt tgggcgtccc agtaagcaat caaatgaaca ctattcaaat
     2821 aaaaacagcg cttcaaagac gaaaaccata acaagacaaa gcagacccca ttttgttcga
     2881 ggcatgcttt catatgttcg atttgatggg catggaagtt tttaaaggga aaagatgttt
     2941 tgttcttggt ttcttttgct tcaaagtcca agtaaaagcc tcgatagacg ccattatagt
     3001 ctgtagttga ggcttgggta aagtaggctt ccttgatcac agcagcactt ctcttaggat
     3061 agtctacttt cacgatttgg ataggggtag gctttttatg gataaccgcc aacccgcggg
     3121 aaagataata tttattactt tcattgattg cttcttcaaa gcgcatcccc cgattgccga
     3181 attcagtgat ctgatttctt ttttcttgaa ttttttcgac gggagaggca ttcttataat
     3241 aaggttggcc atttggatag cgcaatacca tctttaatca cactcctagg ccacattata
     3301 gcaaaaaagc ggatagaaag aaaaggaatc tgagatggat aagtcaaaag taatctatgt
     3361 gtctgggtat cgaagtttcg aactcggtgt ttttaaagaa aatgatccta aaatccaagt
     3421 tgttaaaaat gttttaaaaa aagaactcca tcagttagca gaagaagggc tggaatgggt
     3481 acttgtcagt gggaatttgg gcgtcgaact atgggctgtg caaatagtag cggaattgaa
     3541 agaagaatat ccggaactat ctcttggtat tttgtatcct ttttctgatt ttggtaataa
     3601 ctggaatgaa gggaaccaag aaaaaaaaca attagcagaa caattagcag attatgtcga
     3661 agctgttagc catcaaccct accaatcacc tagtcagcta aagaatcaca ctcgttttat
     3721 acttgagcat acagtgggct gtctgctgat ctatgatgaa gaattccctg gaaaaacgaa
     3781 gtattttttt gaagatgcac agaattacca agcagatcat ccctacgaaa ttcggctgat
     3841 cagtatggac gatcttcaaa attttagtga ataatcgata ttgtgtttga tttatcaact
     3901 tttttcctat acaatgaaaa aggtagaaat gttattacgt aaatgaaaac tagaggtgta
     3961 aatatggcga atttggttta tagtcctaaa gacattttac aacaagaatt caaaacaaaa
     4021 atgcgcggct atgatcccgt tgaagttgat gaattcttag acaatatcat caaagattat
     4081 gaaacttaca gcaaagaact ccttgcttta caagaagaaa acgatcgatt gagtgcaaaa
     4141 gtagctcaat tatctaaaac tcaaggagca gctcaaacac gcgtgcaaca aacagaggca
     4201 ccaaagagcg cagcagtgac caattttgat atcttaaaac gcttgtcaaa tcttgaacgt
     4261 gaagtatttg gtaaaaagct tgatcaacaa gcaagtgcag tcaaaccagc acaacctaat
     4321 ccaaataact acacaaatgc agacacaagc ttagatgata atgaaaaaac acgtcaattt
     4381 taatttttga ttaatgaatt tgcggttttc gggtaatcgc ggtctgactt gatcaggctg
     4441 aggaaagtcc atgctcgcac aggctgtgat gcctgtagtg ttcgtgctta gcgaaaccat
     4501 aagctaaggt actctttgga gtaacggcag gaaaacaagc taaggcttcg gctatgctta
     4561 agtatccttg aaagtgccac agtgacgaag cggctgggga aacccatccg gtggaacgcg
     4621 gtaaacccct cgagcgagca acccaaacaa tggtaggggc gctttttgtc tggaagaaga
     4681 acaggacaaa aaggcagtat aactgcagat agatggttac cgctttgttt tttctccctg
     4741 gaaaagcaaa gtacagaaca tggcttatag aaaaccgcaa acatcaggga attaggtcgt
     4801 gacaaaagta atgcgttata agaaacaaga aaaacatttg aaagctattt ttcaatactt
     4861 cagatgaatc gttcttatta caaaacaaac ttttgtatca ccggttattc ggaatttgag
     4921 taaaagtgtg gggtaggact gatgtcccac ctcctttttt tataaataag gagattgtgt
     4981 atggaaaaaa acaggaagtt caacctcgtc gcaactgcgg caagcgggct agaagcactg
     5041 gttggtaaag aattaagaga tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt
     5101 tttgaaggaa caatggaaac gatcgctaca gctaatttat ggttacgtac agcagatcga
     5161 gtaaaaatcg tggttgggga attcgatgcg tatacgtttg atgaattgtt tgaaaacgta
     5221 aaagcattgc cttgggaaga ttttcttcca ctagatgcag cttttcctgt agctggaaaa
     5281 tcgatcaagt cgaaactata tagcacacct gattgccaag cgatcacaaa aaaagcaatc
     5341 gttgaacgtc tccgtaccta ttatcaccgt ccagcttctg taccactgac ggaaactggc
     5401 gcacattttc agttggaagt agctttgctg aaagaccatg tgatggtaac tctggatacg
     5461 acaggaccaa gtttattcaa acgtggttat cgcttagaaa aaggtggagc accattgaaa
     5521 gaaaacatgg cagcagcact tattatgttg acaaattggc gaaaagaccg tccattttat
     5581 gatcctgtat gtggttcagg tacgctttgt attgaagcag ccttaatagg ccataatatc
     5641 gcaccaggat tcaatcgtga gtttgcttgc gaatcttggg attggttcag tcaagaaatc
     5701 atggaaaatg ttcgatcagc agccgaagaa aaagcagatt atgatattga attagatatt
     5761 actggttcag atatcaatgg acggatgatt gaaattgcca aagccaatgc agaagaaatc
     5821 ggattaggct cttcgattac ctttaagcaa caagcagtca aagacttcaa aacggataaa
     5881 gaatacgggg tcatcgtggc taatccgcct tacggagaac gtctaggaga agaagaaact
     5941 gttcggaaat tatataaaga aatgggcgat gtattccgtc cattaaaaac atggagtaaa
     6001 tatatcttga ctagcgactt agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa
     6061 agaaaattgt acaatggtgc attgcgtacg gatctattcc agtattgggg aactcgtccg
     6121 cctagaaaaa aagagaaaga cgaataatct tactaattga ttcattcaat tggatgtttg
     6181 gaggaatagg gaatgaaaga aaaagagttt ttacaagaag taaaagaaat caatcttcta
     6241 caacaagctt taggaatcct cgattgggat acacaaacag gtatgcctga aaaagctagt
     6301 gcataccgaa gtgaagtaga cagctatctt tacagcattt atttttcaaa aaaaatcggt
     6361 ccaaaaatca aagaagccat tcgctatttt tctgaacatc cggaagaatt gtcggaagtt
     6421 ggaactgccg tttttgccaa agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa
     6481 gatcttatga gaaaatatac agctgccgtt tcttcagcac acggacaatg gcaaaaagct
     6541 agagaaacac aacaattttc tgattttcaa cacgcattga atcaaaatat tgaattaaca
     6601 aaacagttga taccttactg gagaaaagat gaaaagacga attatgatgt tttgctgaat
     6661 caatacgaac ccgggatgac tgtcgaaatc cttgataagg tatttgatca gcttaaagaa
     6721 ggaattcttt cgatccgccg tacgctccaa gaaaaaggga ctgaacctga aacagacttt
     6781 ttaaatcgga gaatgacgaa aaaacaacag aagagatttg tagtgaaagt aatcgaacaa
     6841 cttggttatg attttagtcg tggtcgttta gatgacactg tccacccatt tatgactgga
     6901 attaatccga atgatgcacg aatcacgaca cgatggaacg aaacagattt caaaatggct
     6961 gtttttggag tgatccacga ggctggtcat ggtatgtatg aacaagacat cgatgataaa
     7021 tatgcgtata caccggttta tgaaggtaca tcaatgggta tccatgaatc acaatcgttg
     7081 tttaacgaga tcattattgg aagtagtcgc agcttctgga aaaagcaata tcctttcttt
     7141 caagaatgtg cagaaggaac attcgatgat attccttttg acaaatttta tcgttcattg
     7201 aaccatacaa aatcaagttt ggttcgtatc gaggcagaca gtttgactta ccctctccat
     7261 atcatcattc gttatgaaat cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa
     7321 ctacctgaaa tatggaatca aaaatatgaa gagtatctgg gaatacgacc aacaaatgat
     7381 gtggaaggca tcctacaaga cgttcattgg tcaggtgcaa gctttggtta ttttccctct
     7441 tatgcattag ggtacatgta tgcggcacaa ttacttcatt ctttgcagca agaaatagat
     7501 gtggaaaatg ttctagcgtc agacgattat tctccaatca aaaactggat gtcagagcat
     7561 atccatcaat atggtgcatc tagaaaacca aatcaattaa tcatggatgc aacacatgaa
     7621 ccgcttaatc cgcaatacct gatcgactat atgaaagacc tgtattttaa tgtatatcag
     7681 gtaaaataaa atacaattta caaagatagc tgaaattcat ctaatctata gatgttttca
     7741 gctatttact taagaaagaa gtggaacgaa tggataaatt acaagtgatc gctgattata
     7801 gtatggacca attagcgcat gatcaaacag gacatgggag cgatcatacc aaaagagttg
     7861 tcaagttagc tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg
     7921 cagcagctta cctacatgat acgattgatg ataaagtcgt taaaaatgag aacgaggcaa
     7981 agcaacagct tcgtgtattc cttcgctctt tgccaatagt agaggaacag atctcgatga
     8041 ttttttccat tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg gccgaactat
     8101 ctctggaagg taagatcgtt caagacgctg atcgtatcga agcattagga gcaatcggca
     8161 ttttgcgtac tgcttatttc ggcggtggac atgggcaccc gatttttgat cctgaactgt
     8221 atccgcaaac attcaaggac aaagaaaatt accgaaaagg cacgacagtg atcaatcatt
     8281 tttatgaaaa actgttcttg ctgtctgata aaatgaatac tgattacgga tatgaagaag
     8341 ccaaacgaag agaaaccttc atgcgtgaat tcttagaaga attttttctg gaatggtcta
     8401 ttgacgatga aaattttact ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt
     8461 tgtgacagaa gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa gttacttctg
     8521 gaacactgtt taccaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat
     8581 ttcaaaaaat agcttctcgt gtttgatgaa tattgtaact tgtgattcgg aattggcagg
     8641 agaacaccgt ttattcggaa gaataggact gtgacaagac ttttgtcaca gtcctttttt
     8701 cgaattcaac aagtaaaact tttaagaaat attcagagag taaaattaaa aattgctgat
     8761 aaacgaagat ggaaatttta tgtattttta ttggtctatg caataaataa attaaatatt
     8821 cagaatacaa ttgattttaa cgaaagggct tctatcaatg gttgtgttgt atttatctgc
     8881 ccaggtagag taaaatagat cattatctaa aaggtaattg ttcatactat ataagtcatt
     8941 tgaaagtaaa aagatactgt aaataaaaaa tgtggttaaa taattctcat aatcagtgta
     9001 agcgttatca aaaatatagc atacttgctt tgtagatgaa tgacacgata tatgtttggt
     9061 atgttctgca agacagatga atatggaggt ggagacaatg tcttccaaag aactaacaat
     9121 gtatttagag aaagaattgg atcaactgaa agaaaagggg ttatacaata cgatcgacat
     9181 actagaaagt gaaaatggtg cttgtatcat cgtagacgga aaaaagatga tcaatctagc
     9241 atccaacaat tatcttggat ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc
     9301 aaccgaaact tatggtgtag gagcaggagc agtccgaacg atcaatggtt cgctgaaaat
     9361 ccatcagcaa ttagaagaaa aaatcgcaga attcaaaggg acagaagcag caatcgcctt
     9421 tcaatctgga ttcaactgca atatgggcgc tatctctgca gtaatgacaa aagaagatgc
     9481 tatcttatcg gatgaattaa accatgcatc tattattgac ggttgccgct tatccggagc
     9541 aaaaatcatc cgaattaaac atcaagatat gaaagacctt gagaaaaaag caaaggaagc
     9601 gatagaaagt aagaaatata aaaaaatcat gtatatcaca gatggtgttt tctccatgga
     9661 tggagacatt gcacgattgc cggaaattat tccgattgtt gaaaaatatg gactgatcac
     9721 ctacgtcgat gatgcgcacg gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca
     9781 ttttggatta agcgacaaaa tcgatatgca aatgggcacg ttatcgaaag ctataggtgt
     9841 tgttgggggt tatgtagctg gatcgaaaac actaatcgac tggttgaaag ctagaagtcg
     9901 gccattccta ttttctactt cgctaactcc aggtgcagct gcagctgcat tggcttcgat
     9961 tactttgatg caagaacatc cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt
    10021 taaagaagaa ttgaaaaaag taggctataa catcggtatg tctgaaacac ctattactcc
    10081 agtcattttg ggagacgaaa aagtgactca gactttctct aagaagctga tcgaacatgg
    10141 gatttatgct aaaccaatcg tttatccaac tgtgcctctt gggacaggca ggatcagaaa
    10201 tatgccgact gcggaacata cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa
    10261 aataggaaaa gaaatggaaa ttatttaact aaatttagtt ttttcgtcta tttaaaatta
    10321 gagaacatgt tcacgcatat attgttaatc atctgaagat agtagtaaaa aagatcaggg
    10381 aggatcatca tgaaaaaagt attggtgaca ggatgtttag gacaaattgg ttcagagttg
    10441 atcacacggc tacgtcaaga actaggcagt gaaaatgtca ttgcgacaga tatacgcatg
    10501 cctgaagtca ttgagaaaaa cgatatcttt gagatcctag atgtaatgga ttacgaaaaa
    10561 atgcgcagca tagcagaaca atatcaagtg gacacattga ttcatttggc tgctcttttg
    10621 tcagctgttg cagaagcaaa accacaatta gcctggaatt tgaacgtgac tgggctggtc
    10681 aatgcattgg aagtcgctag agaattccag ctgaaatttt ttacgccaag ttcaatcggc
    10741 gcgtttggtc caaatactcc aaaagatcat accccacaag atactcttca gcgtcctaca
    10801 accatgtatg gggtaacgaa ggtagctgga gaactactat gtgattatta ttatgtgaaa
    10861 tatggcgtag atactcgtgg tgtccgtttc cctggattga tttcttatgc aaccttgcca
    10921 ggaggcggta cgacagacta cgcagtcgat atttattatt cagcaatcaa agaaggaaag
    10981 tacactagtt tcattgatca aggtacatca atggatatga tgtatatgcc agatgcgatc
    11041 gatgccatta taaaactcat gaatgcagat cctgatcgat tggttcatcg aaatgccttc
    11101 aatatatcgg cgatgtcttt tgacccggaa gagatcaaag catctattca aaaaatcata
    11161 ccagattttg aaatggatta tgatgtggat cctgtacgtc agtcaatagc agaatcttgg
    11221 ccaaattcac tagatgtttc ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac
    11281 ttagatgtga tgacaaagga tatgttggaa aaactgaaag aaaaattggt agagacggta
    11341 taaaacaaga accacatcca aaaagttttt tatacttttg ggattggcta tggtatgaaa
    11401 ggggacaagg gatggaagaa tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg
    11461 aattaaaacg tacgatggga ttttttactg ctttatcgac tgttatgggc acagtgatcg
    11521 gtgcaggggt attttttaaa gccgcaagcg tagcagaagt tacaggatcc gccagtttgc
    11581 atatgttttc ttggtttctc ggtggtatga tttctgtctg tgcaggacta acaggagctg
    11641 aactggcagc tgctattcca gaaacaggag ggatgattaa atatatcgaa cggatttacg
    11701 gaaatacagc agctttttta ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg
    11761 tagcagcctt gtcgatcatt ttcggtacac agttcgtcaa tctatttggt ctttcccagt
    11821 caatgattgt tcccgtagct gttacagctg ctgtatcgat cctgttgatc aattttttag
    11881 gttctaaagc tggaggggct tttcagtcga ttacgcttgt ctgcaagctt attcctttgt
    11941 ttgtgatcgt cattttcggt ttgttccgac aagaaggagt agacttccaa ctgtttccga
    12001 tccaagcagg agaaaatctt tcatttttct cagccttggg agcgggcttg ctagcaacga
    12061 tgttcgcata cgacggttgg atacacgtag gaaatatttc gggagaattg aaaaagccgg
    12121 cgaaagattt gccaaaagca atttctttag gaatcatcgg aatcatgatc gtttatttat
    12181 tagtaaatgc ggtgttctta agaaccgcat ctatcgacgg agtcgcagga aacagcaatg
    12241 cggcaagtga cgttgcaaaa atgatttttg gcggcttcgg tggaagactg gtcacagttg
    12301 gtatcttgat ttctgtttat ggaaccatca atggctatac ccttactgga atgcgtctcc
    12361 cttatgttat ggcaaaagaa aacaacctgc cttttagcaa gctctttgct aaacttcatg
    12421 ataaaactaa agttcctgta gccgcaggta tcttagagct agtcatcgct attggcatga
    12481 tgatgatcgg tggttttgat acactaacgg atatgctgat ctttgtcatt tggatttttt
    12541 atacgatggt ctttgttggt gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac
    12601 catacaaagt tccaatgtat ccgtttattc cactagtagc aatcattgga gggactttta
    12661 tcgtatcaag cacgcttatc actcagacct ttttagcttc gatggggata gtattgacac
    12721 ttgctggtat cccgatttat ctctatttaa aacgtagata gctaaaacat tcgaatttta
    12781 ttagaactgc acttctctgc caaacaaaat attttattta aatgaggagt acgtggaaga
    12841 aatgtcaaaa gtactcgtta attttgatca cgtaaatgtc gttttgtcta cgatgaaata
    12901 gcaaagtaaa agacagaaat cgggctcaac cgatttctgt cttttacttt gatttgcgta
    12961 actgaactat tcggcgtacg ccgcgataaa tatttaataa aaccaagcaa ccagaaatga
    13021 aaatgggaag ccaaatcaga tagacagggt agccaagcac tttagacaaa gcatgtggag
    13081 tcattttatc catttcaaga agtgcttgct gccaagtctg aagattgaag actagcatga
    13141 aacccgaaat aatcaggaga ataaatccta aataatgaat tgtagttctt tttcttacat
    13201 aagcgatcgt agcataaagg aacagtaaaa aaataggtac gccaaaaaca aagtacatat
    13261 tcagcatctc gacaccccct ttgtgtttat cttactttac tttgaaagaa atgagaaata
    13321 atagcctcgt tacttgcttg aaacagcaaa ggactgctaa gataagagag atacaaagaa
    13381 tcaaggagga gtcatatggc aagttatatc gaaccgattc gttttgcgct gattgttttt
    13441 ccatttttag ccttagcgat ctcatctgtt ttttttattt atgaatatcg aaagtatgga
    13501 acatttctag taacaagggc agttattttg tattctttcg ttttttattt attgtgtgcg
    13561 tattttttgg tgatcttacc acttccggca aaggagacgg ttgcccaaat gacagggcca
    13621 aagatggagc tgcatttatt tgcttcatgg caacatttca gagacaacac ggtattggtc
    13681 ttgtctgaac ctagtaccta cctgccggca atgaagcaga gtgtattttt ggaaccagct
    13741 tttaatgttt tattactcat accatttggc atttatttaa gatattattt ccggctttct
    13801 tttatgaaaa cgatgattgc tagtttctgc ctttctctat tttttgaatt gacgcagcta
    13861 agtggacttt attttatcta cccaagacca taccgcttat ttgatgtgaa tgatttagtg
    13921 acaaatacat tgggtggaat cattggtttt gtcgttacgc cgttatttac atttatgctt
    13981 cctacaagaa gacgaatgga tgaactttct tatgaaaaag gaaagactgt ctcattgact
    14041 cgacgcttgg tcgcttattt gatcgactgg acatttttat ctcttattaa tttggtagcg
    14101 gcgattttct ttaaaattgc gttcgattca ggggcagtta cttctcatat ctggtggctt
    14161 ttattagaag taggggtata ctttatgatt gtaccgttta taaccaatgg atggacactt
    14221 ggcaaaaaaa ttgtgcgtat ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa
    14281 gcattgatga tccgctacgg gtatctgtac tttctttttt atggtttaag ctggttgggg
    14341 cagttgcttc agcaaatcat gaattcgaca acgaatacgg tgttgcagat gagtgcagta
    14401 ttcatttctc tcggtgtctt gttactacaa ggtattttta ttttaaatat cttgctatca
    14461 tttttgagaa aaaaacggat tctgttttat gaaaaagcta gtcacacata tactgttagc
    14521 acgattggcc aaacaaagga tgatgattct cgaaattagc tgagcaaaaa cgctttgtct
    14581 gtgttataat gccaaagtcg tgataattta aagagaagag gtgcacgaat gaataatttt
    14641 gatatattcc gcttcaaacc gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag
    14701 cccacagagg tccaggaacg tttgattcct ttaattgcaa aaggaaaaaa tatcattggg
    14761 caatctcaaa ctggtacagg aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc
    14821 aatgcagaaa aagaggaagt acaagttgtt attacagctc caagtaggga actagctgca
    14881 cagatctatg aagcagctac acaaatcgct aaattttcta caccggagat ccgtgtctcg
    14941 aattttgtcg gcggtacaga caaacagcgt caattaaatc gtctgcatca ccaacagcca
    15001 caggttgtag taggcacgcc tggtagaata ttagacatga tgaatgaaca agcgctgaag
    15061 attcacaccg catttgcatt tgttgtcgat gaagcggata tgacattgga tatgggtttt
    15121 ttaaatgaag tagaccaaat tgcaagtcga ttaccagaaa agctgcagtt tttagttttt
    15181 tctgccacta ttccagaaaa acttcgtcca ttcttacgaa aatacatgga aaatccaatc
    15241 attgaagaaa tcaaaccaaa agcagttatt tctgaagaca ttgaaaattg gctgatttca
    15301 acaaaaggta aagatgagaa tcaaatgatt tatcaactgc tgacaattgg tcatccttat
    15361 ttagcaatcg tatttgccaa tacaaaacag catgtggatg aaattgccga ttacttgaaa
    15421 aaccaaggct tgaaagtagc aaagattcat ggagacatca ccccaagaga gagaaaacga
    15481 gtgatgcgac aagtacagaa tctggagtat caatatgttg tagcgactga tttagctgct
    15541 agagggatcg atatcgaagg tgtctctcat gttattaatg cagaaattcc agaagatcta
    15601 gacttcttta tccatcgtgt cggacgtact ggacgtaatg gcttaaaagg aacggccatt
    15661 acgttatatt cgccaaatga tgagcaagcc attgatgcga tagaaaaatt gggcgtctca
    15721 tttgaaccaa aagaaatcaa aaacggggaa atcgtcaaaa catacgatcg taatagacgt
    15781 gccaaacgag aaaaatcaaa agatgtatta gatcccacgc tgattggtct tgtgaagaag
    15841 aaaaagaaaa agatcaagcc tggttataag aaaaaaatcg attgggcaat tgcagaaaaa
    15901 aataaaaaag aacgcaaaat cgaacgcaga cagcaaagcc gtactgctcg taaaaataag
    15961 aaaaattcac ataaa
//
LOCUS       DADXTH010000069        15400 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.069, whole genome shotgun sequence.
ACCESSION   DADXTH010000069 DADXTH010000000
VERSION     DADXTH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.069"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(291..1787)
                     /gene="lysS"
                     /locus_tag="IWI97_002129"
     CDS             complement(291..1787)
                     /gene="lysS"
                     /locus_tag="IWI97_002129"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBC2463622.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="IWI97_002130"
     CDS             complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="IWI97_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBC2463623.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="IWI97_002131"
     CDS             complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="IWI97_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBC2463624.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="IWI97_002132"
     CDS             complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="IWI97_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBC2463625.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="IWI97_002133"
     CDS             complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="IWI97_002133"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBC2463626.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="IWI97_002134"
     CDS             complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="IWI97_002134"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBC2463627.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8318..8788)
                     /locus_tag="IWI97_002135"
     CDS             complement(8318..8788)
                     /locus_tag="IWI97_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBC2463628.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8834..9259)
                     /locus_tag="IWI97_002136"
     CDS             complement(8834..9259)
                     /locus_tag="IWI97_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBC2463629.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9326..9595)
                     /locus_tag="IWI97_002137"
     CDS             complement(9326..9595)
                     /locus_tag="IWI97_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBC2463630.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9606..11201)
                     /locus_tag="IWI97_002138"
     CDS             complement(9606..11201)
                     /locus_tag="IWI97_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBC2463631.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="IWI97_002139"
     CDS             complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="IWI97_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBC2463632.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14754..15314)
                     /locus_tag="IWI97_002140"
     CDS             complement(14754..15314)
                     /locus_tag="IWI97_002140"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBC2463633.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3975 a         3468 c         2717 g         5240 t
ORIGIN      
        1 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
       61 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      121 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      181 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      241 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      301 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      361 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      421 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      481 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      541 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      601 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      661 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      721 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
      781 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
      841 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
      901 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
      961 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1021 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1081 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1141 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1201 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1261 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1321 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1381 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1441 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1501 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1561 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1621 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1681 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1741 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     1801 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     1861 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     1921 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     1981 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2041 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2101 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttctcctg
     2161 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2221 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2281 catccccgtt ccccataaat gggatcgtca aatgtttttt cacttctttc aatacttccc
     2341 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2401 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2461 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2521 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2581 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2641 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2701 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     2761 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     2821 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     2881 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     2941 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3001 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3061 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3121 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3181 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3241 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3301 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3361 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3421 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3481 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3541 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3601 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3661 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3721 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     3781 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     3841 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     3901 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     3961 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4021 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4081 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4141 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4201 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4261 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4321 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4381 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4441 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4501 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4561 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4621 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4681 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4741 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     4801 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     4861 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     4921 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     4981 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5041 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5101 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5161 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5221 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5281 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5341 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5401 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5461 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5521 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5581 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5641 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5701 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     5761 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     5821 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     5881 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     5941 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6001 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6061 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6121 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6181 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6241 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6301 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6361 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6421 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6481 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6541 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6601 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6661 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6721 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     6781 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     6841 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     6901 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     6961 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7021 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7081 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7141 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7201 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7261 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7321 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7381 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7441 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7501 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7561 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7621 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7681 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7741 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     7801 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     7861 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     7921 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     7981 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8041 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8101 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8161 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8221 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8281 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8341 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8401 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8461 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8521 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8581 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8641 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8701 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     8761 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     8821 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     8881 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     8941 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9001 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9061 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9121 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9181 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9241 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9301 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9361 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9421 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9481 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9541 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9601 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9661 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9721 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
     9781 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
     9841 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
     9901 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
     9961 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10021 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10081 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10141 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10201 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10261 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10321 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10381 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10441 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10501 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10561 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10621 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10681 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10741 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    10801 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    10861 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    10921 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    10981 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11041 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11101 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11161 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11221 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11281 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11341 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11401 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11461 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11521 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11581 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11641 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11701 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    11761 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    11821 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    11881 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    11941 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12001 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12061 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12121 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12181 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12241 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12301 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12361 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12421 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12481 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12541 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12601 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12661 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12721 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    12781 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    12841 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    12901 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    12961 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13021 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13081 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13141 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13201 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13261 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13321 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13381 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13441 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13501 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13561 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13621 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13681 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13741 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    13801 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    13861 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    13921 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    13981 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14041 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14101 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14161 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14221 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14281 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14341 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14401 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14461 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14521 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14581 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14641 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14701 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    14761 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    14821 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    14881 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    14941 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15001 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15061 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15121 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15181 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15241 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15301 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15361 gctaattagc ggtccagtcc atcgagcttt tttctttttt
//
LOCUS       DADXTH010000070        13809 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.070, whole genome shotgun sequence.
ACCESSION   DADXTH010000070 DADXTH010000000
VERSION     DADXTH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.070"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWI97_002141"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWI97_002141"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBC2463634.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="IWI97_002142"
     CDS             complement(2262..2513)
                     /locus_tag="IWI97_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBC2463635.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="IWI97_002143"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="IWI97_002143"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBC2463636.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="IWI97_002144"
     CDS             3463..3681
                     /locus_tag="IWI97_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463637.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="IWI97_002145"
     CDS             complement(3970..4482)
                     /locus_tag="IWI97_002145"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBC2463638.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IWI97_002146"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IWI97_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBC2463639.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="IWI97_002147"
     CDS             complement(6935..7378)
                     /locus_tag="IWI97_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBC2463640.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="IWI97_002148"
     CDS             complement(7379..8167)
                     /locus_tag="IWI97_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2463641.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="IWI97_002149"
     CDS             complement(8164..9105)
                     /locus_tag="IWI97_002149"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBC2463642.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="IWI97_002150"
     CDS             complement(9282..9869)
                     /locus_tag="IWI97_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBC2463643.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="IWI97_002151"
     CDS             complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="IWI97_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBC2463644.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="IWI97_002152"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="IWI97_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBC2463645.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="IWI97_002153"
     CDS             complement(12786..13721)
                     /locus_tag="IWI97_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2463646.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3806 a         3096 c         2435 g         4472 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa tatcatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgta aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataac
//
LOCUS       DADXTH010000071        13678 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.071, whole genome shotgun sequence.
ACCESSION   DADXTH010000071 DADXTH010000000
VERSION     DADXTH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.071"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IWI97_002154"
     CDS             complement(<1..902)
                     /locus_tag="IWI97_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBC2463647.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IWI97_002155"
     CDS             1185..2153
                     /locus_tag="IWI97_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2463648.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IWI97_002156"
     CDS             2266..2544
                     /locus_tag="IWI97_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBC2463649.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IWI97_002157"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IWI97_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBC2463650.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="IWI97_002158"
     CDS             3001..3756
                     /locus_tag="IWI97_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBC2463651.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IWI97_002159"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IWI97_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBC2463652.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IWI97_002160"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IWI97_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBC2463653.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IWI97_002161"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IWI97_002161"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBC2463654.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IWI97_002162"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IWI97_002162"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBC2463655.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IWI97_002163"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IWI97_002163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBC2463656.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IWI97_002164"
     CDS             8493..9035
                     /locus_tag="IWI97_002164"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBC2463657.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IWI97_002165"
     CDS             9058..10614
                     /locus_tag="IWI97_002165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBC2463658.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IWI97_002166"
     CDS             10628..11530
                     /locus_tag="IWI97_002166"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBC2463659.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IWI97_002167"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IWI97_002167"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBC2463660.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IWI97_002168"
     CDS             12973..13395
                     /locus_tag="IWI97_002168"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBC2463661.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4520 a         2448 c         2981 g         3729 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca
     9901 ggtcgtgaag cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgcctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaagta attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgctgctc agccgtgctg tgaagaaaac tggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccgccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattacaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggggaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca
    13561 gaggttgtaa aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatt
//
LOCUS       DADXTH010000072        13636 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.072, whole genome shotgun sequence.
ACCESSION   DADXTH010000072 DADXTH010000000
VERSION     DADXTH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.072"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            37..279
                     /gene="yaaA"
                     /locus_tag="IWI97_002169"
     CDS             37..279
                     /gene="yaaA"
                     /locus_tag="IWI97_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBC2463662.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            266..1390
                     /gene="recF"
                     /locus_tag="IWI97_002170"
     CDS             266..1390
                     /gene="recF"
                     /locus_tag="IWI97_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBC2463663.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1387..3333
                     /gene="gyrB"
                     /locus_tag="IWI97_002171"
     CDS             1387..3333
                     /gene="gyrB"
                     /locus_tag="IWI97_002171"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBC2463664.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3353..5824
                     /gene="gyrA"
                     /locus_tag="IWI97_002172"
     CDS             3353..5824
                     /gene="gyrA"
                     /locus_tag="IWI97_002172"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBC2463665.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6060..6359
                     /locus_tag="IWI97_002173"
     CDS             6060..6359
                     /locus_tag="IWI97_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBC2463666.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6409..6921
                     /gene="ssb"
                     /locus_tag="IWI97_002174"
     CDS             6409..6921
                     /gene="ssb"
                     /locus_tag="IWI97_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBC2463667.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6947..7183
                     /locus_tag="IWI97_002175"
     CDS             6947..7183
                     /locus_tag="IWI97_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBC2463668.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7326..9299
                     /locus_tag="IWI97_002176"
     CDS             7326..9299
                     /locus_tag="IWI97_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBC2463669.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGESAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9305..9757
                     /gene="rplI"
                     /locus_tag="IWI97_002177"
     CDS             9305..9757
                     /gene="rplI"
                     /locus_tag="IWI97_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBC2463670.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10031..11398
                     /gene="dnaB"
                     /locus_tag="IWI97_002178"
     CDS             10031..11398
                     /gene="dnaB"
                     /locus_tag="IWI97_002178"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBC2463671.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12178..13470
                     /locus_tag="IWI97_002179"
     CDS             12178..13470
                     /locus_tag="IWI97_002179"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBC2463672.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4540 a         2465 c         3008 g         3623 t
ORIGIN      
        1 gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt cattctaaat
       61 acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg tagtggcgga
      121 caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc agaaaatcgt
      181 cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg aacttttttt
      241 atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa aaaattatcg
      301 aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt taggagaaaa
      361 tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga cgcgaagtca
      421 tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga tgattcaagg
      481 agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta aaaaaggccg
      541 aaaaaccaag atcaaccata tcgaacagaa aaagttaagt acttacgtgg gacaaatgaa
      601 tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc agaccaggcg
      661 gaagttttta gatatggaat taggccagat caatccaatc tatctttatg atcttgtcca
      721 ataccaagca attttgaaac aaagaaacca gtacttgaag caattgaatg aaaaaaaaca
      781 aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg gcagcaaagt
      841 attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt ctttgcatca
      901 gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag tcgattcttt
      961 agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga accaaaataa
     1021 aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg atctttcctt
     1081 ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa gaacaactgc
     1141 actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacagggg aatatccaat
     1201 ccttttgtta gatgatgtga tgagcgagct ggatgataat cgtcaactcc atctgctaga
     1261 aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc atgtaaaaaa
     1321 taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa aaggagaaga
     1381 aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga tgccagtcag
     1441 attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta tatcggttcg
     1501 actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc gatcgatgaa
     1561 gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa cagtatcacc
     1621 gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg acgtcctgca
     1681 gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg tgggtacaaa
     1741 gtttctggtg gacttcacgg agtaggatct tctgttgtta acgcgctttc tacacagctt
     1801 gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg tggagctgta
     1861 gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt tcactttacg
     1921 ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt ggctacccgg
     1981 atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga caaacgagaa
     2041 gaagaaccag tcatcaatga ataccattat gacggcggga ttaaaagtta tgtcgaatac
     2101 ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg tgaacagcaa
     2161 gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa tatcatgagt
     2221 tttgccaaca atatccatac gtatgaaggc ggaacacatg aatcaggatt caagactgct
     2281 ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga aaatgacgac
     2341 aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat caagcatcct
     2401 gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt acggacagtc
     2461 actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc aagtgtgggc
     2521 cgtcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc gaaacgtgcg
     2581 cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg aaaattagcg
     2641 gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg agattcggcc
     2701 ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc tattcgtggg
     2761 aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga agagatccgg
     2821 tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc gaaagctcgc
     2881 taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat ccgtacattg
     2941 ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata cgtctatatt
     3001 gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt acaaccagga
     3061 aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc accaaaacca
     3121 agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg ggaaacaact
     3181 atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat tgcagcagat
     3241 cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt catcgaggaa
     3301 aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa gaatgagtga
     3361 agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag actccttcat
     3421 cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag atggtctgaa
     3481 acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac cggataaagc
     3541 ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc ctcatggaga
     3601 ctatgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc gttatatgct
     3661 agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg ctatgcgtta
     3721 taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca ataagaacac
     3781 tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac tacctgctcg
     3841 cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg ccacgaatat
     3901 cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg ataatcctga
     3961 tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa caggtggttt
     4021 ggttatggga aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt ctattaccgt
     4081 acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca tcgtgacaga
     4141 attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac tgcacagaga
     4201 caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag gtatgcggat
     4261 cgtcattgac atccgtcgtg acgccagcgc atctgttatc ttgaataact tatataaatt
     4321 gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag gtgtgccgaa
     4381 agttttgagc ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg aagtcatcac
     4441 aagacggaca gtctttgaaa aagaaaaagc agaagcaagg gcacacattt tagaaggttt
     4501 gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt ctaaatcaga
     4561 tgacgaagca aaagcgacga tgatcgaacg gttcgattta tccgatcgcc agtctcaagc
     4621 tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa tcgaaaatga
     4681 ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa gaccagaacg
     4741 tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg gtgataaacg
     4801 acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt taatcgaaga
     4861 agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg caaataatga
     4921 attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc acaatgacga
     4981 cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct ttacgaataa
     5041 cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgca cagccaaagg
     5101 aataccaatt atcaatttac taggtatcga ttcgagtgaa agcatccagg caatcattgc
     5161 ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc aaggtacagt
     5221 aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga tcgcaattgg
     5281 tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat ctaatatcat
     5341 tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc gcgatatggg
     5401 acgtacagct tccggggtta gaggaatacg cctgcgtgag gaagattatg tcgttggggc
     5461 agccttaatg aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt acggaaaacg
     5521 gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca aaacggcgaa
     5581 catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag atgaagacat
     5641 catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg tttctcaaac
     5701 tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga aagttgtcac
     5761 gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag gatcagaaga
     5821 atagccatca agactaattg tgtaagctag caagaatgac cgattaaaaa atctggttat
     5881 tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact gttcattctg
     5941 tattgctttt gaccaaaata gagtgtataa tcagagcttg tgagtagtct aattaattag
     6001 ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg tgaatatcaa
     6061 tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat gaagaagcaa
     6121 aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca gaagttatcg
     6181 aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac cgtgaaggta
     6241 tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat gaatttgacc
     6301 gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa gaagcttaat
     6361 tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt gattaataat
     6421 gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc aagtggtacg
     6481 gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag cggcaatcgc
     6541 gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt agcaaactat
     6601 gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc ttatgataat
     6661 cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca attgctggaa
     6721 agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc tggtcaatca
     6781 gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc caacggtatg
     6841 cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat cgacatttca
     6901 gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg cacaacaaag
     6961 aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata ttgaatatat
     7021 cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta aaatcttgcc
     7081 acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg caatcaaacg
     7141 cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc taggaattga
     7201 atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat aacctctatt
     7261 cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata tatgggagga
     7321 aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt atcctgttta
     7381 tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc gcttcggtca
     7441 ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt tggcttattt
     7501 taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat ctagcttatg
     7561 tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa aaaatcgaat
     7621 ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag gaagaattgc
     7681 tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta gcagataaaa
     7741 catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat attacccaaa
     7801 accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt ttatctattg
     7861 ataactatgc agatgccatt gataaattgg atgacaaaga gatttcttat ttgaactctt
     7921 tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc aaaagaatca
     7981 acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg atggataaaa
     8041 aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg cctatcaccc
     8101 ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga gaagctcaga
     8161 ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa gaggtaaaag
     8221 aaggagcaaa acctgtttat tatggggggg aatctgctaa gaatgtcaaa cgcacgcgtg
     8281 ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca ggcgatgtgt
     8341 ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt ggtgtcagtt
     8401 gtctggcacg cttcaaccag aaaaaagctt acatcgttat caacgagaac gagatcattc
     8461 cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa atgcaactaa
     8521 tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg atggtggatt
     8581 accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat aagattgtga
     8641 tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg acttatatcg
     8701 aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac caaagcagca
     8761 aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg gttgtagata
     8821 caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc tatttaaggt
     8881 cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta gcgacatact
     8941 tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt gttgtggcgg
     9001 cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca gatacattgc
     9061 tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac ggcttgattg
     9121 ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa tccttaggtg
     9181 gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt gaggaaacaa
     9241 aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat aaggagtaat
     9301 ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag gggacatcaa
     9361 agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg caaaagaagc
     9421 aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa aacacgaagc
     9481 tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag atactgtagt
     9541 cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc cttcaaaaca
     9601 aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa aaatcgaatt
     9661 agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc atcatgaggt
     9721 gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag cgatgttcag
     9781 ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga aaacagctgc
     9841 agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct tttttgggaa
     9901 cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt tattacactt
     9961 tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt gaagacaagg
    10021 agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata ttgaagctga
    10081 acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg ccatggagta
    10141 catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga caatgatcga
    10201 gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc tggaacaagc
    10261 caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat tagcagttcc
    10321 gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat tgctgcgtac
    10381 attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag aagaagttca
    10441 aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa gaaatcgaag
    10501 cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg atcgactagc
    10561 tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg ataaaatgac
    10621 agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg taggtaaaac
    10681 agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt ctgttgcgat
    10741 atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg cagaaggttc
    10801 gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt cgaatctgat
    10861 cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac cagggatcaa
    10921 aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca atctaggctt
    10981 gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc gtcaacaaga
    11041 agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag tacctgtcat
    11101 tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac cagtgctaag
    11161 tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct tcttgtatcg
    11221 agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc caaaaaatga
    11281 caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa cagttgaact
    11341 actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag aattttaaag
    11401 aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca aggagtaacc
    11461 gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg acaaaagtct
    11521 tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg gtattagctc
    11581 agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac tcaggtcaaa
    11641 acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc ctcggtcaca
    11701 agtcacaata tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg tgactcagag
    11761 ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc atctgcggta
    11821 atacgcccaa tcaaacgaaa aatatgagaa gctatttttg tttggctgtt cttattactt
    11881 gatgcaggac agctttttca caacctctga ttcacggtgt tccaaaagta gcttcttcga
    11941 aaaataagtc gaaatttcac aaaaatttga gaagcaattt tcgtacattc cttcttattt
    12001 cttgaagctg accacttctg ttacaacctc ttttttaata accattctaa aaagataaag
    12061 ttcgtttttt aagcgttttt ataaaataaa aataaaaatc attcgatttt atttgaaata
    12121 tgtatctatt attgatacaa tggttcagga ttaaataata tagaacgagg tgttcacatg
    12181 tcatctgtag tagtagtagg tacgcaatgg ggcgatgaag ggaaaggaaa aatcacagac
    12241 tttctcagtg aaaatgctga agtgattgca cggtaccaag gtggcgacaa tgcagggcac
    12301 acaattaagt ttaacggtgt gacttataaa ctgcatttga ttccttctgg gattttttat
    12361 aaagacaaga tcagtgtgat cggaaacggc gttgtagtca atcctaaatc attggttaaa
    12421 gaattagctt atttacatga aaaaggtgta gaaacaagta atttgcgtat ttctgaccgc
    12481 gcgcacgtaa tcttgcctta tcatatcaaa ttggaccaat tacaagaaga tgctaagggc
    12541 gaaaataaga ttggtacaac gattaaagga atcggacctg cctatatgga taaagcagct
    12601 cgtgtaggta tccgtattgc agacttatta gataaagaaa tttttgaaga acgtcttcgt
    12661 ttgaatctag atgacaaaaa ccgtcaattt gtaaaaatgt ttgattcaga accacttgat
    12721 tttgacgata tttttgaaga atattatgaa tacggacaac aaatcaaaca atacgtcaca
    12781 gatacttccg ttatcttgaa tgatgctttg gatgcaggca aacgcgtgtt gtttgaaggc
    12841 gcacaaggtg taatgctgga tatcgaccag ggaacttatc cttttgttac ttcttctaac
    12901 ccagtagccg gtggggtaac aatcggaagc ggtgtagggc cttcaaaaat caataaagtt
    12961 gtcggcgtct gcaaagccta tacgtcacgt gtcggtgatg gaccatttcc aactgaatta
    13021 tttgatgaaa caggagaaca aatccgcgaa gtaggacgag aatacggtac gacaactggt
    13081 cgtccgcgtc gtgtaggctg gtttgattct gtagttatgc gccattctaa acgagtttca
    13141 ggtattacca acctatcttt aaactcgatc gatgtattaa gtggattgga aacagtaaaa
    13201 atttgtacgg cttatgaatt agatggcgaa ctaatctacc actatccagc aagcttgaaa
    13261 gagttgaacc gctgcaaacc aatctatgaa gaattgccag gctggtcaga agacattaca
    13321 ggatgcaaaa cacttgcaga actgccagaa aacgcacgta actatgtacg tcgtatctca
    13381 gagcttgttg gtgtacgtat ctcaacattt tctgtcggac ctgatagaac acaaacaaac
    13441 atcttagaaa gcgtctgggc gcaaatctaa ggataaaaac gacgactgaa tcaggacgtt
    13501 cttaccagag cgagtcgaag tgtttatcta tacgataata ataacttgaa gaggtagtga
    13561 aagagacgtt tagccttatt gccgataggc tgtctcttga acactgttta ttcagaataa
    13621 gggactgtga caaaaa
//
LOCUS       DADXTH010000073        13402 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.073, whole genome shotgun sequence.
ACCESSION   DADXTH010000073 DADXTH010000000
VERSION     DADXTH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.073"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            136..309
                     /locus_tag="IWI97_002180"
     CDS             136..309
                     /locus_tag="IWI97_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463673.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="IWI97_002181"
     CDS             949..1422
                     /locus_tag="IWI97_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBC2463674.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWI97_002182"
     CDS             1431..1658
                     /locus_tag="IWI97_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2463675.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IWI97_002183"
     CDS             2294..2665
                     /locus_tag="IWI97_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463676.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IWI97_002184"
     CDS             complement(2921..3163)
                     /locus_tag="IWI97_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBC2463677.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IWI97_002185"
     CDS             complement(3195..4097)
                     /locus_tag="IWI97_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBC2463678.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IWI97_002186"
     CDS             complement(4110..4298)
                     /locus_tag="IWI97_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBC2463679.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWI97_002187"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWI97_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBC2463680.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IWI97_002188"
     CDS             complement(4913..5806)
                     /locus_tag="IWI97_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2463681.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="IWI97_002189"
     CDS             complement(5884..6807)
                     /locus_tag="IWI97_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBC2463682.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IWI97_002190"
     CDS             complement(6856..7206)
                     /locus_tag="IWI97_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBC2463683.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IWI97_002191"
     CDS             complement(7239..8141)
                     /locus_tag="IWI97_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBC2463684.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IWI97_002192"
     CDS             complement(8134..8991)
                     /locus_tag="IWI97_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBC2463685.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IWI97_002193"
     CDS             9325..10134
                     /locus_tag="IWI97_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2463686.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IWI97_002194"
     CDS             10174..10671
                     /locus_tag="IWI97_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2463687.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="IWI97_002195"
     tRNA            complement(11056..11129)
                     /locus_tag="IWI97_002195"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="IWI97_002196"
     CDS             11316..11639
                     /locus_tag="IWI97_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463688.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="IWI97_002197"
     CDS             11803..12057
                     /locus_tag="IWI97_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463689.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="IWI97_002198"
     CDS             12127..12372
                     /locus_tag="IWI97_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2463690.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="IWI97_002199"
     CDS             12448..12813
                     /locus_tag="IWI97_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463691.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3905 a         2475 c         2244 g         4778 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa ag
//
LOCUS       DADXTH010000074        13147 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.074, whole genome shotgun sequence.
ACCESSION   DADXTH010000074 DADXTH010000000
VERSION     DADXTH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.074"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(79..151)
                     /locus_tag="IWI97_002200"
     tRNA            complement(79..151)
                     /locus_tag="IWI97_002200"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="IWI97_002201"
     CDS             532..1101
                     /locus_tag="IWI97_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBC2463692.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="IWI97_002202"
     CDS             1088..1948
                     /locus_tag="IWI97_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBC2463693.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /locus_tag="IWI97_002203"
     CDS             2138..2842
                     /locus_tag="IWI97_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2463694.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="IWI97_002204"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="IWI97_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBC2463695.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /locus_tag="IWI97_002205"
     CDS             4679..5995
                     /locus_tag="IWI97_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463696.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="IWI97_002206"
     CDS             5996..6865
                     /locus_tag="IWI97_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBC2463697.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="IWI97_002207"
     CDS             6920..7729
                     /locus_tag="IWI97_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBC2463698.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="IWI97_002208"
     CDS             complement(7832..8461)
                     /locus_tag="IWI97_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBC2463699.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="IWI97_002209"
     CDS             8631..9923
                     /locus_tag="IWI97_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2463700.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="IWI97_002210"
     CDS             complement(9999..10661)
                     /locus_tag="IWI97_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBC2463701.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /gene="groES"
                     /locus_tag="IWI97_002211"
     CDS             10886..11170
                     /gene="groES"
                     /locus_tag="IWI97_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBC2463702.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="IWI97_002212"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="IWI97_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBC2463703.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4371 a         2264 c         2833 g         3679 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    12841 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
    12901 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
    12961 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
    13021 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttgtgtg
    13081 taattataat gaaaatagtt atttttaata gttataatat tttatctatt aaaagtaaat
    13141 atccatt
//
LOCUS       DADXTH010000075        13125 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.075, whole genome shotgun sequence.
ACCESSION   DADXTH010000075 DADXTH010000000
VERSION     DADXTH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.075"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            157..555
                     /gene="merR"
                     /locus_tag="IWI97_002213"
     CDS             157..555
                     /gene="merR"
                     /locus_tag="IWI97_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HBC2463704.1"
                     /translation="MAYFISSFSEKCGVNKESIRYYERKNLLPEPDRTNSGYRVYSEE
                     DVKRVKFIKHLQEIGFSLNEIYKLLGVVDKDNIRCEGMFEFVSKKEREIQKKIENLNR
                     IEYMLKDLRARCPDEKELYACPIIEVLIEN"
     gene            569..2209
                     /gene="merA"
                     /locus_tag="IWI97_002214"
     CDS             569..2209
                     /gene="merA"
                     /locus_tag="IWI97_002214"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HBC2463705.1"
                     /translation="MNQFRMRIQGMTCTGCEKHIEKALESMGALNAKADFSQSEAVFQ
                     LPDDYAIENAIEAVKKINYQPEEVEKLPVQKRVMTNNKEDYDLLIIGSGGAAFSAAIK
                     AVEYGSKVGMIERGTVGGTCVNIGCIPSKTLLRAGEINQSAHVNIFEGLHTSTGEVEL
                     DRLVDQKNELVDDLRKQKYTNLIDDYGFDLIKGEAKFIDENTVTVSGQTYSAQRFLIA
                     TGASPLLPRITGLKEVDYLTSTTLLELRKVPKRLTIIGSGYIGRELGQLFHNLGSEVT
                     LIQRSKRLLKEYDPEISEAMEKALIEQGIKLISGATYERVEQDGNIKKVHIIVNGKNK
                     VIESDELLVGTGRKPNTEVLNLSAAGVQVGPNNEIKINAFAQTSNRKIYAAGDVTLGP
                     QFVYVAAYEGGIAAGNAIGGLNKAIDLSVVPGVSFTSPQFATVGLTQQQAKEKGYEVK
                     KAVLSLENVPRAIVNHNTTGVFKLVVDRKTQKILGVHIVSENAGEVIYGASLAVKFGL
                     TVADLKDTLAPYLTMSEGLKLAALAFDKDISKLSCCAG"
     gene            complement(2535..2807)
                     /locus_tag="IWI97_002215"
     CDS             complement(2535..2807)
                     /locus_tag="IWI97_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBC2463706.1"
                     /translation="MSSGYQVEFEKGAQKALKKMDKYQSLLIMGWIQKNLVNCTDPRQ
                     HGKGLTVNRSGEWRYRIGDYRLIADINDETVTILMLEIGHRKNIYK"
     gene            complement(2808..3029)
                     /locus_tag="IWI97_002216"
     CDS             complement(2808..3029)
                     /locus_tag="IWI97_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBC2463707.1"
                     /translation="MTVISLRIDTQEDKLIKEYAKAKNISVSALIRSSVLEKIEDEID
                     LGLYNQAMKDHKENPKSISFEDMLKELSD"
     gene            complement(3123..5030)
                     /locus_tag="IWI97_002217"
     CDS             complement(3123..5030)
                     /locus_tag="IWI97_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBC2463708.1"
                     /translation="MKEIQEQHSHENNSHDHGKMPIILYFIGLALSLIALFVNGENNL
                     IQNTLFSIASISAGYHVIILEGIGETIENSKRQHKFTPNSHILMGIAAIGASLIGNFW
                     EGTLLVLIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLIMQDGNTTIVNVSELKVG
                     DQLQVLNGDQVPIDGVIVSGSTSIDESSINGESIPKEKSKGDGVFGSTINGTGTFTME
                     VTKETNDTVFSKILQLVNQNQENQTKASSIIQKFEPKYVKFVLIAIPLFILLAPMLFD
                     LTWSQSIYRGLVLLVAASPCALAAATVSVTLSATSNLAKRGVLSKGSAYLSQLADIQA
                     IAFDKTGTLTKGKPEVTNYYFSDSVNEENMIDIVVALEKESNHPLASAILDKFEPKNK
                     LAIEVENQIGKGLIGDYNENTYRIGKPTSFEAVSDDYTRLNKEWASEGNTVVYVSENE
                     KVIGLIALMDIPSEHAKATIDYFKKCGIHTTLITGDSEMTGQAVGKQLGVDQVIANVM
                     PEDKSNIINEQKETYGITAMVGDGINDAPALVKADVGIAMGEGTDVAVDVSDLVLMKN
                     DLSKLVKAHEISLKMNRVIWQNIIFSMVVVAFLITVSLVGLTDIAISVIVHEGSTLVV
                     ILNGLRLLKSK"
     gene            5286..5606
                     /locus_tag="IWI97_002218"
     CDS             5286..5606
                     /locus_tag="IWI97_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBC2463709.1"
                     /translation="MNSLSTSPLKNESIQEVSKIFKMISDPTRLSILFLLQKEELSVG
                     AIAHSLSMEQSAISHQLKTLKTSRLVKSKRAGKNMIYSLDDLHIFSILEQVSTHIEEQ
                     EKEK"
     gene            complement(5718..6332)
                     /locus_tag="IWI97_002219"
     CDS             complement(5718..6332)
                     /locus_tag="IWI97_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010620830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HBC2463710.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGLRQIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVALVGEEEEEEEEVVEKLE
                     SRGTNRFFWTVALITIASGGDNLGIYIPYFASLSFSEIVTALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERILVPIVFIGLGIYILIENGTIQTLLNFI"
     gene            complement(6349..6710)
                     /locus_tag="IWI97_002220"
                     /pseudo
     CDS             complement(6349..6710)
                     /locus_tag="IWI97_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(7020..8924)
                     /locus_tag="IWI97_002221"
     CDS             complement(7020..8924)
                     /locus_tag="IWI97_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBC2463711.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDIKRFEQHGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGHMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            complement(9122..9355)
                     /locus_tag="IWI97_002222"
     CDS             complement(9122..9355)
                     /locus_tag="IWI97_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBC2463712.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            complement(9502..10356)
                     /locus_tag="IWI97_002223"
     CDS             complement(9502..10356)
                     /locus_tag="IWI97_002223"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBC2463713.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTVIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            complement(10341..10679)
                     /locus_tag="IWI97_002224"
     CDS             complement(10341..10679)
                     /locus_tag="IWI97_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463714.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNIVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            complement(10907..11611)
                     /locus_tag="IWI97_002225"
     CDS             complement(10907..11611)
                     /locus_tag="IWI97_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBC2463715.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            complement(11756..12328)
                     /locus_tag="IWI97_002226"
     CDS             complement(11756..12328)
                     /locus_tag="IWI97_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBC2463716.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            complement(12962..>13125)
                     /locus_tag="IWI97_002227"
     CDS             complement(12962..>13125)
                     /locus_tag="IWI97_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2463717.1"
                     /translation="VNQTFISSVSNQRNHIPRESLNYRTPIEIFLSYVQEALYSSLIC
                     QIIYSQTII"
BASE COUNT         4145 a         2573 c         2176 g         4231 t
ORIGIN      
        1 ataaaagaag tctcaaaatt tattggtacc acatttaatt ttcacttcat ttttgaaagt
       61 aaataagaag gaaaatgata ttcccccctt cgtattattt ttggttggac atgtacctaa
      121 gtacatggtt tataattaaa aaaaggatgt gatagcatgg cttattttat tagctccttc
      181 tctgaaaaat gtggtgtaaa taaggaatcc attcgatatt acgaacgaaa aaacttacta
      241 ccagaacccg atcggactaa ttccggttat cgagtttatt cagaggaaga tgtaaaacga
      301 gtaaaattca ttaaacacct acaagaaatt ggcttttctt taaatgaaat ttataaatta
      361 cttggtgttg tagataaaga caacattcgt tgtgaaggta tgtttgaatt tgtctctaag
      421 aaagaaagag aaattcaaaa aaaaatcgag aatttgaata gaatagaata tatgctaaaa
      481 gatttacgag caagatgtcc agatgaaaaa gaactatacg cgtgtcctat catagaagta
      541 ttaatagaaa attaacgggg gtaggaaaat gaatcaattt agaatgcgca ttcaaggaat
      601 gacctgtact ggctgtgaaa aacatataga aaaagcgctt gagagcatgg gagctctcaa
      661 cgctaaagca gatttcagtc aaagcgaagc ggtatttcaa ttaccagacg actatgcaat
      721 agaaaacgcg atagaagcag ttaaaaaaat aaattatcag cccgaagaag tagaaaagtt
      781 acctgttcaa aaacgcgtga tgactaataa caaggaagat tatgatttgc tcattatcgg
      841 ttctggtgga gcagcttttt cagcagctat aaaagcagtc gagtatggat caaaggtggg
      901 catgattgaa cgcggaactg taggcggaac ctgtgtgaac attggttgta tcccttcaaa
      961 aacgctgctt cgagctggag aaatcaatca atcagcacat gtaaatattt ttgaaggatt
     1021 gcacacttct acgggagaag tagaattaga ccgcttagtc gatcaaaaaa atgaactagt
     1081 tgatgattta cgcaaacaaa aatacactaa tttaattgac gactatggtt ttgatttaat
     1141 taaaggagaa gcaaaattca ttgacgaaaa cactgtgaca gttagtgggc aaacatattc
     1201 tgcccaacgg tttttaatcg caacgggtgc ttctccactc ttacctcgta ttactggact
     1261 taaagaagta gattatttaa ctagcacaac cctccttgaa ctaagaaaag taccaaaacg
     1321 attaacaatt attggatcgg gttatattgg aagagaactg ggacagctct tccacaatct
     1381 gggttctgag gttaccctta ttcaaagaag caaacgactt ttaaaagagt atgatccaga
     1441 gatttctgaa gcaatggaga aagctttaat cgaacaagga ataaagctca tttccggagc
     1501 aacttatgaa cgagtagagc aagacggaaa tattaaaaaa gttcatatta ttgtaaacgg
     1561 caaaaataag gtcattgagt cagacgagct actagttgga acaggaagaa aaccgaatac
     1621 agaagtattg aacttgagcg cagcaggcgt tcaagttggg ccaaacaatg aaatcaagat
     1681 aaatgctttt gctcagacaa gtaatagaaa aatttatgca gcaggagacg tgacattagg
     1741 acctcagttt gtctatgtag cagcctatga aggaggaatt gcagcaggaa atgcaattgg
     1801 tgggttaaat aaagcaatcg atttatcggt agttcctgga gtatcattta ccagtccgca
     1861 atttgcaact gttgggttaa cacaacaaca agcgaaagaa aaaggatacg aagtaaaaaa
     1921 agcggtattg tcattagaaa atgttcctag agcgattgtc aatcataata cgacaggggt
     1981 gtttaaactt gtggttgata gaaaaacaca gaagattttg ggagttcata tcgtttctga
     2041 aaatgcagga gaagttattt atggagcatc cttagccgtt aaatttggat tgaccgtagc
     2101 tgatttaaaa gatactttag cgccttatct aacaatgtca gaaggcttaa agttagcagc
     2161 tctagccttt gataaagata tttcgaaatt atcttgttgt gcaggttaat atttctattt
     2221 agtttcttgt agtagctggt aacaccttgg tttatctttc gttattatct ataacgatag
     2281 gagttgaaga ttatgacatc agcaaacgtc tacgtgccaa ttaatagctc tattctagtg
     2341 gaggttgaag aaataaaaga ctatttagaa acgcaattgc tcatttaggc aacgcagcaa
     2401 gatattattg aagatgcgat tgttgcctta cgtgcttaat atagataatt aaatcgtatc
     2461 aaaaaaggat tctaccgctt aaaaagcagt agaatccttt tttattaaac atgcttgcac
     2521 tcaatttgaa tggtttattt gtaaatattt tttctatgac caatctctaa cattaaaatg
     2581 gttacggtct cgtcattgat atcagctatt aagcgataat caccgattct atagcgccat
     2641 tcgcctgatc gattgacggt taatcctttt ccatgctgcc taggatcggt acaattcact
     2701 agattctttt gaatccatcc cataattaaa agggattggt atttgtccat tttttttaac
     2761 gctttttgag ctcctttttc aaactcgact tgataacctg agctcattta atcagatagc
     2821 tcctttagca tgtcttcaaa agaaatactt ttcggattct ctttatgatc cttcatggct
     2881 tgattatata ggcccaaatc aatttcatcc tcgatttttt ccaagacaga acttctaatc
     2941 agagccgaaa ccgaaatatt cttcgcctta gcatattctt taattaattt atcttcctga
     3001 gtatcaattc tgagcgatat gacagtcatt agaatcaccc ccttcttttt gtatatacat
     3061 tgtaatacaa aattagagag gctgcaaact ttagatacat gattttatcc aatttcatgt
     3121 atttatttag actttaataa tcgaagacca tttaatatca cgactaaggt acttccttca
     3181 tgaacaatca cactaatggc tatatccgtt aatcctacta agcttacggt aattagaaaa
     3241 gcgacaacta ccattgagaa aataatattt tgccaaataa ctcgattcat ttttaaagaa
     3301 atttcatgag cttttaccaa tttagacaaa tcatttttca ttaatactag atcagataca
     3361 tctactgcaa catccgtacc ctctcccata gcaattccaa catctgcctt cacaagagca
     3421 ggtgcgtcgt ttataccatc tcccaccata gcggttatac cataggtttc tttttgttca
     3481 ttgataatgt tagatttatc ttctggcatg acgtttgcaa ttacttgatc tactcctaat
     3541 tgtttaccaa cagcttgccc tgtcatttca gaatccccag taattaaagt ggtatggata
     3601 ccacacttct taaagtaatc tatagttgct ttcgcatgtt cacttggaat atccattaat
     3661 gctatcaggc cgattacttt ttcattttct gatacatata caaccgtatt accttctgaa
     3721 gcccattctt tattcaaacg tgtatagtca tccgatacag cctcaaaaga agttggttta
     3781 cctatacgat aagtgttttc gttataatcg cctattaaac cttttccaat ctgattttcc
     3841 acttcaatag ctagtttatt ttttggttca aacttgtcta aaatagcact tgctaacggg
     3901 tgatttgatt ctttttcaag agccactacg atatcaatca tattttcctc gttcacagaa
     3961 tcagaaaagt aataattggt tacttccggt ttccctttag ttaatgttcc tgttttatca
     4021 aaggcaatgg cttgaatatc cgctaattgg gacaaatagg cacttccttt tgaaagtacg
     4081 cctctttttg ctagattaga agtggctgac aatgttacgg atacggtagc tgctgctaaa
     4141 gcacacggag aagctgcgac taaaaggact aatcctctat aaatactttg tgaccatgtc
     4201 aaatcaaaca gcataggagc aagtaaaatg aagagtggga ttgcaattaa aacaaattta
     4261 acatacttag gctcaaactt ttggataata ctagacgctt ttgtttgatt ctcttgattt
     4321 tgattcacta gttgtaaaat ctttgaaaag acagtatcgt tagtttcttt tgtgacttcc
     4381 atagtaaagg tacccgttcc attaattgta ctcccaaaaa ctccgtctcc ctttgatttc
     4441 tctttaggta tactctcacc attaatagaa gattcgtcaa tcgaagtaga gcctgatact
     4501 attactccgt caatcggtac ttggtcaccg tttaacactt gaagttgatc tcctactttc
     4561 aactcactaa cattaacgat tgttgtattg ccatcttgca tgattagcct agctgttgtt
     4621 ggattcattt ctaataactt ggtaatttct cttttacttc ttccttcagc ataatcttca
     4681 agaaaatgtg ctccagagaa aataagaacc aataacgttc cttcccaaaa atttcctatc
     4741 agagaagcac ctattgcagc tattcccatt aaaatatgag aattaggggt aaatttgtgt
     4801 tgtctttttg agttttcaat cgtttctcca attccttcaa gaataatgac atgataacca
     4861 gcactaattg aagcaattga aaacaaagtg ttttggatta aattattttc tccgtttaca
     4921 aataacgcaa tgagcgacaa tgctaaacca ataaagtaca aaataattgg cattttccca
     4981 tgatcgtgac tattattttc gtgtgagtgt tgctcttgaa tctctttcat ttctatcacc
     5041 tttcttaata aaactttttt ttagaaatcg taatgcttac gaattacgtt tgaacgaaca
     5101 ttcatatgtc tatacattca ttttatatcg ctctatctaa tttgtcaaaa aaatagttat
     5161 cttatttagg tgataaaaaa aataggactc taaaatagct acccttatac aaaaaatctt
     5221 gctttataaa aatattatga taatctaaat gtgaataaac aaacatatga aggagagaaa
     5281 ttcttatgaa ttcattatca acttctccat taaaaaatga atctatccaa gaggtaagta
     5341 aaatatttaa aatgatcagt gaccctaccc gactttcaat tttatttctt ctgcagaaag
     5401 aagaacttag cgttggagct attgcgcatt ctttgagtat ggaacaatca gcaatttctc
     5461 accagttaaa aactttaaag acttcaagat tagtaaaatc aaaaagagct ggtaaaaata
     5521 tgatttatag ccttgacgat cttcatattt ttagtattct tgagcaagtt tcaactcata
     5581 tcgaagaaca agaaaaagaa aaataaagac aaactacaag gttaaaatga ttttacaatt
     5641 cattagttca aataaaaaaa gacagcgagt taaaatctcg ctgtcttttt ttatttgaac
     5701 ttcactatcg tagttgttta tataaaattt aatagtgttt gaatcgtccc attttctatc
     5761 aaaatataaa ttcctaagcc aataaacact ataggaacaa ggattcgttc atatttttct
     5821 aaggtttcgg aaacaaaaga aatcttggct aatttgtaac tgatatagca aagaaccgca
     5881 acagaaatag caaatacgat tagagcggtg acaatctctg aaaaagataa cgaggcaaag
     5941 taaggaatat aaatccctaa gttatcgcca ccagaagcaa ttgttattaa ggcaacggtc
     6001 caaaagaagc ggtttgttcc tcttgattca agcttttcaa cgacttcctc ttcttcttcc
     6061 tcctcttcgc caaccaatgc cactctgatt cctaagaaaa tcggaattag accgagaagc
     6121 ccaatgatcc aatcttgcgg aatgaaattc agcacatatg ctgcaaataa acttacggct
     6181 actaagatac ctgttcctag atattgaccc caaaaaatct gacggagccc tttttttgta
     6241 tgactttgag aaaaaataat cagcaagata aacaaataat caatgctggt agaaatataa
     6301 acagcgatag ctgaaaaaat actttgtaac aaaataactt cctccaaact aagatttcat
     6361 agatagattt tctgataact ttaaaaaaac atgaatagcg gaaaggatgt cttcattcgc
     6421 taaagagtaa taaactactt ttccatcttg acgtgatttt gccattccat gtttttttaa
     6481 aaaacgtaaa tggtgtgacg ttgtagcgac actagcatta agtatttctg ctaaatcaca
     6541 gacacacatt tccttatagg tttctaaagc ataaaaaatt ttaattctag aagaatcgct
     6601 gaaacatttc cctaacacaa gtaagtttga aaaatctttt tgatccaatt tttttttatt
     6661 acatttactt tttctttatg tacgatagtc acttggcaaa ctttttccat tcatctcacc
     6721 tcacattcaa acggtcgttt gaaggtagta tatctatttg atcactcgtt tgtcaatcta
     6781 ccttaatttc aatacactat tataagcaat ttcatgtgtt acaacttaat aggaatttaa
     6841 attttattat taaatttttt aatcatttac acgaaaatta attttataaa gaacatactc
     6901 tatactctta ttttttttca agaactcaaa aaagcggaaa atcccagatt taaaaatctg
     6961 agtttccgct tttgtcattt caaggatgga ctattgtctc agcctcttct aatttattct
     7021 taagatattt ttactttctc tgcaggtgct gatacttctt tttgttcttt tcgacctttc
     7081 aacttatact tcattaatct catgccattg aaaattacca ccaagatact accttcatga
     7141 accaacattc cgatagacat attcatccat tcgctgaaga agacgctggt caacaagacc
     7201 aacactactc caattgaaat cacgatattt tgtttcatat tatttgcggt ggcttttact
     7261 aatcctaatg cgtgaggcaa gttgctgaag ttcgagttca ttaaaacgac atcggatgtt
     7321 tcaatcgcta cgtccgttcc gcttcccatt gcgattccaa tgtctgctaa agctaaggac
     7381 ggactatcgt taacgccatc tccaacaaag gctacaattt gaccacgttg ttgaagtttt
     7441 ccgatataag ccgatttgtc ttccggcaac atgtggccgt gagcttcggt caaaccaagt
     7501 tcgccggcga ccacatcaac ggttccttgg ttatctcctg aaagaacgac tagatttttc
     7561 acgcccaagt cttttaattc ctgcaaatta gctttcacac ccggacggac ttgatcgcga
     7621 atgcccatca gtacttttaa ttcaccgtcg accgctgtca aaacgagaga gttcccatgt
     7681 tgttcaaacc gtttgatatc tttttgcacc tttttgctga gagtgacatt ttctttttcc
     7741 atcaaggcca cattcccaac agccactcta tgtccagcta cacttgaaac gattccgccg
     7801 cctttcacaa cttcagttcc ttcaacagga taaaagtttg tttctccaat ttgatttaag
     7861 accgcttttg ctaatggatg atctgattcc cgttccacac tggccagata gccaagcgtt
     7921 tcagcaggat ctttttcata cagttcagtc gctgcaacgg ttgggtttcc gacagttaaa
     7981 gtacctgtct tatcaaatac aattgtatcc acattactga agtcttgaat gacttcactc
     8041 ccttttaaaa gaacaccgtt acgagcacca ttcccaatac cggcaacgtt tgagacaggc
     8101 actccgataa ctaatgcgcc tgggcaacct agaaccaaga tggtgattgc taactcaata
     8161 ttttgactga acgcccatac aacaattgcc agaaccaaga cagctggtgt gtagtattta
     8221 gaaaagcgat caatgaaccg ttccgcttcg gatttagaat cttgtgcttc ttccacgagt
     8281 tcaataattt ttccaaatgt ggtgtcctca ccaactcggt cagctctgat ttgaatcgtt
     8341 ccgttttcta aaatggttcc tgcaaaaact tctgcatcag ctagcttgtt gaccggaata
     8401 gcttcacctg taatgctagc ttcattgata tgcccttcac ccgtaaggac tatgccatct
     8461 actggaactt tcgcacccgt tttaacgagc aagatatccc cttcatctat atcatccact
     8521 tctacttctt caaattcgcc attatccatt tgtttcaagg cgctttcggg tgccatttca
     8581 gtcaattctt tgatagcaga tcgcgtttgg ttcaatgttc gttgttccaa gtagtgtccg
     8641 aataagaata agaacgtgac aatagccgat tcttcataat tttgaataaa aagcgcacca
     8701 atcgctgcaa tactgactaa gacatcaata ctgatgactt tgactttcat tgcttgaaac
     8761 gcttgaatcg caattggcgt aatcccaaag acagaggcga tgatcaaaga ccactctgaa
     8821 agtcctacgt tttctaaaac aaagtgactg aagaaaccga gtacgattaa caatccactg
     8881 atgacggtga taacattttt cttgcttaat atatattgtt gcatgctttt tccctccaaa
     8941 tttaatttct cttttattga taaacacagt ataagataat tgcctaagta attaattgac
     9001 tgacatcaag tttagaaagt tctcttttat cgtttctaag cacctcgaat cggattgcaa
     9061 caaaaaaagc aatcctggaa aaatagcttt tcttccagaa ttgcctagtt catttaatct
     9121 gttaaacggc cttgactttt gctttaagaa ccggatagcc tacgttttcg atagcttttt
     9181 gaatctcttc aatcgaagtg acagctgaat caaaattggt tttgactttg ctggagttga
     9241 ataatacttt aatgctgtct ttatcaatac cgttaactga tttcacggca ccttcaattt
     9301 tttgcataca acttggacaa gacaacgttt ctaattgcaa tatagctttt tccataattc
     9361 ctatctcctt tatgtgtttt tattttatcg gcagcagatt aatgaacact gccttttcct
     9421 ctttacaact gtagtatagc tcacttttag caaacgaaaa ttgacgcata tcaagttttg
     9481 cacttctttt tatctttttg ctcaattcac catcttaata gtctttttct gccccttatt
     9541 gccatcagaa taataagaaa ttggtggata atcttgtctg cgtataaacc atactacaat
     9601 cgcgccaata aacagggcca gagacaaggc ttgcgaaacg cggatccact cgccaatcca
     9661 taaactgtcc gttcgcatac cttcaatgaa gaaccgaccc actgagtacc acaaaacgta
     9721 actcaaagcg acttctcctt gacgcaaaag ctgttttcgg ttccgtatag tgacgatgag
     9781 gacaaagccc aacaaactcc acaatgactc gtataaaaaa gtcggatggt agtaggtacc
     9841 attcatattc atttgttcaa tgataaattc aggtaaataa agattttcca agaattgacg
     9901 agtaaccggc ccaccatgag cttcctgatt cataaaattg ccccaacgac caatggactg
     9961 ggctaacaat acattgggcg ctaaaatatc aagaacgagt gccaaagaag tacctttttt
    10021 ctttgcatac caatatagcg taccccctcc tgcaatcaat cccccataaa tcgcaatgcc
    10081 gccattccaa atcgcaagga tttgtcctgg attttgcaga taataaccaa gttcaaataa
    10141 cacataataa agccgagcgc cgaggagtcc aatcggaatt gcccataagg ccatatcgat
    10201 aacggtatcc tcttccagtc cttttttctt tgcttccttg gtgctcaact ggagagccgc
    10261 taatatgccc gctgcaataa tgactccata ccaatgaatc acaacagaac caatctccac
    10321 cacatgggga ttcaaaacgc ctaatacgaa tcccattcta tttgctccat ttcttcaatt
    10381 tttctcgtca ttctagtcat ggtgatgctg aacaagattc cggctaagct ccatggaaac
    10441 caaacagtga atacactttt atctccctgg tcactcgctt cgtaatacgc cacttttccg
    10501 tgtccgatta cgccggctcc ttttacaact tcctcttctt tttccatagc taacctatct
    10561 gcctcaacaa tgttagacgt ttttgcttcg ccgccaatcg tgttcttggt gaaagggacg
    10621 acaaagaatg cccccgctgc tgacaacgtg atggcaacta tcacccaaaa acaatgcaca
    10681 ttcaccctac ttatttttac gttttgttcg acaaccctat cttaaagaaa aataaaacaa
    10741 aaaaacttga gggttatcac gtttgtgctt ttatttttcc aatgaatgaa ggtcgttctg
    10801 tcttaaaaaa gccaaaaacg gacagatgga agcaaaagca ctgagttctg tcccgttgtt
    10861 aatgaagagc gacagaacac caacgtgtag aaaaaagtgt gtccgattat tcgctgtaaa
    10921 gcaataaatc atctaaatcc tgaatcttga tccttttccc agacagctgt tgaatcaatc
    10981 cttcgtcctc caattctcca aatttgcgac tgatggtctc aggtgtcgtt cctaaatagg
    11041 aagcaatgtc cttttttgcc atcggcaggg tgatggtggg agagttgccc atttcaggtt
    11101 ccacattttc tgccaaaaac aaaactaaac gggtaatgac actctccacg gctacttgtg
    11161 tcgtttgacg ttctgaactt tctaaccgtt tagacatttc cgccaatagt tttaaagaaa
    11221 tagctgggta ttgttccaga aaatcttgga catctttttg ctgaatcata cagacaacgg
    11281 tatctcgggt tgcttcggca tattcctcgt gtactgcatc cgggttgaac aaggtccatt
    11341 ctccggtgaa atcaccaggg ttcaagatcc gcactagctg ctctttgcca gagtctgcca
    11401 atcgataaat acggattttc ccacgattga taatatataa agtatcctct ttttcttgtg
    11461 aacgaaacag aaactctcct ttttgatact gtttcgtgtg ggctttttca gcaatgtaat
    11521 tcatcgcgct atcctctaag tgattgaata ttggaaccaa gcggatacag gctgcgtggt
    11581 cgccaatttc atgatgggta tgtttagtca attcattctc cccctttatt tattttgttt
    11641 atattcgctg cttttattat ataagtctct tactacaaaa aaattgatgc acatcaagaa
    11701 aatggaaaaa ggactggaaa gaattccaat ccctaaatca gcttctactt ttaccttaat
    11761 cttcatcctc atcaagagcg tcatcatctt ctaatgcttc tttaccaatg aacgcttgca
    11821 gcatccaaat gttcttgtct aagtcatttt taaaagcaat caatgtatct tctaaagcat
    11881 cgtcaccctc gtcttgagct aaacgaatcg cgcgaatagt taggtcacga gtgcttctga
    11941 agtcctcgat aatattttca accatgtctt ctgctttagc gtatttatta actgcatctt
    12001 ccgaaagcat cgaatatttt tcaaattcaa ccgttgtcga agcaggttta tgcccagaag
    12061 taattagacg ctccgcaagt ttgtcgaacc atttttcatt ttcgttatac aactcttcaa
    12121 atttttcatg caacgtaaag aaattggctc cttttacata ccagtgatat tggtgcagtt
    12181 tcacatgtaa tgtatgcatg ttgcctaaaa gatgatccgc aatggccgct gcattaattt
    12241 tcgtatgatg aacatgttcc ttgtgttctt gttcttcttg caatctttct tggggtgttc
    12301 tttcttgagt agctttattt tcagtcatga tttattcctc ctataatttt ggtttgttta
    12361 aaatagcttt ctttgattcc tcctttttct ttactgaatt tagtatagga tagatttccg
    12421 ttaaaaaaat tgacgctcat caagaaaatt atttcttaat agacgccagg cttctctatt
    12481 aaattcaact gaaataatca aaggaatgaa gtcgttccct ccaaacaaaa atttcattaa
    12541 agtagggcct aaaatgtcat ttttatactt aattggcaca gttttttcta attatttgtc
    12601 atattcatag tcaactatag ctacaaaagc aactaatttt ggagatagat tgagatattt
    12661 aaaaatctct catgaaaaat tagttgcttt tgtcacttag cgctgaaaat ttatgccatt
    12721 tatctagaaa aatgacaacc atgtttttca atgttattat gatgtctatt tagggtatac
    12781 cttaaacata catgaaatag tttttgtaat taataaaatt catagaaaca caaaacaaca
    12841 atctatttct aagtttaaca aagtaataga ttgttgtttt gtgttgaata gtttgcatac
    12901 atagttggaa agtaaaggtt agctaaaaaa tgtttgaaaa tgtaattaat ttatttgtaa
    12961 cttagattat agtctgggaa tatatgattt gacaaattaa gctagaataa agtgcttctt
    13021 gtacatagct caaaaatatt tcgattggtg ttctgtaatt caatgattct cttggaatat
    13081 gattacgttg attgctgaca ctggaaataa atgtctgatt cactt
//
LOCUS       DADXTH010000076        13113 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.076, whole genome shotgun sequence.
ACCESSION   DADXTH010000076 DADXTH010000000
VERSION     DADXTH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.076"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            337..2502
                     /gene="acm"
                     /locus_tag="IWI97_002228"
     CDS             337..2502
                     /gene="acm"
                     /locus_tag="IWI97_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBC2463718.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2774..3259)
                     /locus_tag="IWI97_002229"
     CDS             complement(2774..3259)
                     /locus_tag="IWI97_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBC2463719.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3349..3694
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3739..4380
                     /locus_tag="IWI97_002230"
     CDS             3739..4380
                     /locus_tag="IWI97_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBC2463720.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4399..5034
                     /locus_tag="IWI97_002231"
     CDS             4399..5034
                     /locus_tag="IWI97_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBC2463721.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5052..5867
                     /locus_tag="IWI97_002232"
     CDS             5052..5867
                     /locus_tag="IWI97_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2463722.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6186..7622
                     /locus_tag="IWI97_002233"
     CDS             6186..7622
                     /locus_tag="IWI97_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2463723.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7811..10258
                     /locus_tag="IWI97_002234"
     CDS             7811..10258
                     /locus_tag="IWI97_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBC2463724.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10488..11555
                     /gene="rlmN"
                     /locus_tag="IWI97_002235"
     CDS             10488..11555
                     /gene="rlmN"
                     /locus_tag="IWI97_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBC2463725.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11748..12851)
                     /locus_tag="IWI97_002236"
     CDS             complement(11748..12851)
                     /locus_tag="IWI97_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBC2463726.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4505 a         2170 c         2769 g         3669 t
ORIGIN      
        1 tagtataaac taataatgtt aattaaaagt gtattttatg aaactatcgt tatttattaa
       61 tgttgcattt ttgtaagtta aaaattggaa atatagattg aaaaaatgaa agcaataagt
      121 aaaataaaat gaatatatgc gaaaaaataa tttacattag gaaaaacctt gtaaaaacag
      181 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
      241 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      301 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      361 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      421 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      481 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      541 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      601 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      661 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      721 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      781 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      841 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      901 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      961 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
     1021 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
     1081 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
     1141 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1201 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1261 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1321 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1381 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1441 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1501 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1561 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1621 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1681 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1741 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1801 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1861 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1921 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1981 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     2041 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     2101 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     2161 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2221 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2281 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2341 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2401 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact
     2461 atctgtgctg ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt
     2521 tgttaaatat gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca
     2581 tttttatata tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa
     2641 gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa
     2701 gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa
     2761 aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct
     2821 tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta
     2881 cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt
     2941 gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt
     3001 cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg
     3061 cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata
     3121 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
     3181 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
     3241 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
     3301 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
     3361 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
     3421 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
     3481 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
     3541 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
     3601 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
     3661 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
     3721 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
     3781 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
     3841 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
     3901 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
     3961 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
     4021 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
     4081 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     4141 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     4201 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     4261 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     4321 actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     4381 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     4441 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     4501 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     4561 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     4621 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     4681 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     4741 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     4801 ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg
     4861 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     4921 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     4981 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     5041 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     5101 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     5161 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     5221 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     5281 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     5341 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     5401 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     5461 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     5521 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     5581 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     5641 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     5701 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     5761 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     5821 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     5881 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     5941 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     6001 gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt
     6061 tcttactact cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt
     6121 gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga
     6181 gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt
     6241 ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg
     6301 atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag
     6361 accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt
     6421 accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag
     6481 ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta
     6541 tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg
     6601 aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc
     6661 gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt
     6721 atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc
     6781 atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg
     6841 gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa
     6901 atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata
     6961 tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa
     7021 cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca
     7081 attactatca aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa
     7141 tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa
     7201 aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat
     7261 taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa
     7321 atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa
     7381 tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag
     7441 tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa
     7501 agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat
     7561 ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt
     7621 agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa
     7681 atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt
     7741 ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga
     7801 gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac
     7861 gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat
     7921 tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg
     7981 ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg
     8041 attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg
     8101 cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact
     8161 ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa
     8221 acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt
     8281 tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg
     8341 aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat
     8401 tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc
     8461 ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc
     8521 aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca
     8581 aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag
     8641 ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt
     8701 accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga
     8761 tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt
     8821 accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa
     8881 aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa
     8941 ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg
     9001 ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa
     9061 agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa
     9121 tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga
     9181 taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct
     9241 ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat
     9301 taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat
     9361 gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc
     9421 ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga
     9481 agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt
     9541 tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt
     9601 gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat
     9661 tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg
     9721 tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt
     9781 tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct
     9841 cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca
     9901 aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca
     9961 agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc
    10021 agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac
    10081 ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt
    10141 agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa
    10201 agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa
    10261 cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc
    10321 aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag
    10381 tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa
    10441 tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt
    10501 ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa
    10561 aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag
    10621 aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc
    10681 ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac
    10741 tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt
    10801 gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca
    10861 aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt
    10921 attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg
    10981 agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag
    11041 gattggcaat tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca
    11101 aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata
    11161 atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg
    11221 aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt
    11281 tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata
    11341 agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat
    11401 attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg
    11461 taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc
    11521 gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt
    11581 accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat
    11641 tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat
    11701 gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc
    11761 ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt
    11821 ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct
    11881 ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact
    11941 ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc
    12001 atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt
    12061 ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg
    12121 ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt
    12181 gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga
    12241 cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg
    12301 ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat
    12361 aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt
    12421 tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca
    12481 gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt
    12541 tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct
    12601 gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc
    12661 ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt
    12721 tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg
    12781 acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca
    12841 ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg
    12901 atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga
    12961 ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa
    13021 aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt
    13081 tgcttttttg taattatcta aaaattctaa aaa
//
LOCUS       DADXTH010000077        12876 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.077, whole genome shotgun sequence.
ACCESSION   DADXTH010000077 DADXTH010000000
VERSION     DADXTH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.077"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            126..2849
                     /locus_tag="IWI97_002237"
     CDS             126..2849
                     /locus_tag="IWI97_002237"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2463727.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            2977..3399
                     /locus_tag="IWI97_002238"
     CDS             2977..3399
                     /locus_tag="IWI97_002238"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HBC2463728.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            3417..3887
                     /locus_tag="IWI97_002239"
     CDS             3417..3887
                     /locus_tag="IWI97_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2463729.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            3911..4693
                     /locus_tag="IWI97_002240"
     CDS             3911..4693
                     /locus_tag="IWI97_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2463730.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            4677..5507
                     /locus_tag="IWI97_002241"
     CDS             4677..5507
                     /locus_tag="IWI97_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014093338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBC2463731.1"
                     /translation="MKMTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMM
                     PALEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTI
                     TSIKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLR
                     YFGVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQG
                     DEATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT
                     "
     gene            5542..6540
                     /locus_tag="IWI97_002242"
     CDS             5542..6540
                     /locus_tag="IWI97_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2463732.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            6557..7411
                     /locus_tag="IWI97_002243"
     CDS             6557..7411
                     /locus_tag="IWI97_002243"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463733.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(7985..9235)
                     /locus_tag="IWI97_002244"
                     /pseudo
     CDS             complement(7985..9235)
                     /locus_tag="IWI97_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            9752..9943
                     /locus_tag="IWI97_002245"
     CDS             9752..9943
                     /locus_tag="IWI97_002245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463734.1"
                     /translation="MGKYSFEFKSTIVQEYLEGEGGLGYLAKIQGVRSKKGTRMDQSK
                     VRPKNLTFGGRYLYGKISF"
     gene            9924..11335
                     /locus_tag="IWI97_002246"
     CDS             join(9924..10479,10479..11335)
                     /locus_tag="IWI97_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBC2463735.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNDYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            11410..12693
                     /locus_tag="IWI97_002247"
                     /pseudo
     CDS             11410..12693
                     /locus_tag="IWI97_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4309 a         2002 c         2587 g         3978 t
ORIGIN      
        1 taaaaagtgt caaatatttt ttaggaaaaa taccataggt attgttaaag tggaaagatg
       61 tttataaaga atattgaatt ttatagtgaa aatcatttat tattaagtaa aggaggtggg
      121 ttcatttgaa aacgaaaatt ttaaaagaat taaatgatct acttaacagc tatgattttc
      181 cacaatattt tccagaagaa tgtaacgcgc aatatattgc ccaaaaattt caaataaaaa
      241 gaaatacggc tagtaagcat ctaaacgaac taatggaaca gaaaaaagtt gtgaaaatca
      301 atggtcgtcc tgttcttttt tttccagtcc atacaattga acggtatata tcacattcat
      361 tagatagttt tgagttttct tcaattgatt cgtttagaga aaaaatttca aagaaaaaag
      421 cagatcattt ttttgatgat ttaattggtg caaaacaaag tttaaaagaa gcggttcaac
      481 aagggatatc agcaatgaaa tatccaggtg gattaccaat aatgtatttt ggatcgtccg
      541 gagttgggaa gagttactta gtggaaaaac tagctgagta ttgccgacaa actggtatta
      601 ttcatgagaa tgctccgttc atcgagttaa actgcgctca gtattttaat aatccagagt
      661 tattaacaag tcagttgttt ggccatatga agggatcgtt taccggcgcc gattctgacc
      721 atattggtat aatagaagca gcagacggtg gaatcgtctt tcttgacgaa attcatcgtt
      781 taccaccaga aggacaagag aaacttttta ttcatatgga taaaggcgtt tttcgaagag
      841 ttggagatag tggtccttgg cgtttatcac atgttagata tatttttgcg acaacggaac
      901 aggaagaaaa tttttttcta gaaacttttc gacgaagaat tccgattgtt tgtactttgc
      961 ctaattacca agatagagaa attagcgaac gaaaagagat tgtcttccat ttattagaga
     1021 aagagtctac tctgatcaat aaagaaatat tagtttctga agccgccctt agtttcctga
     1081 ttcgcttaga tttgtatgga aacattggtg agctggatgg actaataaaa caaactattg
     1141 cgaataaact atttagtgga agcaataaaa aatatatcga aattcatatt tctgatttac
     1201 ctagcaggta cttgctaaca gaaaatattg agttaaaaaa ccaaattgcg agccaacggt
     1261 tctatatctt tagtaatggt aactatgttc atgattgtga cgagacttca actgtaacta
     1321 caaatttacg aggtttgatt catcgtgttt ctaaatatta ttcagagtgt catgatgtga
     1381 atagaaatag tgaagagttt cgacgttttg tgaaggacca aatggcagaa tttaatgatc
     1441 gttttattta ccaaaaaaat tcagaagtct tagttaagta ttatttaccg gaaatccaaa
     1501 atattgttca aaaaatctct cccagtcttt ttgttcacct ttctggcaat acggtccaag
     1561 ctatagctac ctatctcgct attagggatc aatgggggta tgggaaagag aatgcttcag
     1621 cgaatgaaat tgaaaagtta tatgaaaacc taaagtgtaa ctatttgacg gatttaaatg
     1681 tattcttact gatcttcgag aacgccctgg atacaacatt gactatttgg gataaactgt
     1741 atatcagttt gtgtgttatt agtgagaccg gaaatgaagc gaaagacatt aacgcaatta
     1801 ttttagccca tggatatgcg acagctagta gtattgctaa tgttgccaat cgtttggtta
     1861 atcagtttgt catagattct attgatatgc ctctagatat cagctttagt gaagtaattg
     1921 acagattact tcactatata gagtatcgaa agcctaaaga tggactagtg attttcgttg
     1981 atatgggaag tttagctcaa attaagactg aaattgaaca agtaatcgaa gttccaacaa
     2041 tgattattaa taatgttaca accgaaatgg ctattgaaac cgcacaactg attcaaagta
     2101 cgagtgatat ccaaaaggtt gtaaaaaaat tgccgtattc tcagtttgaa aagcaagttc
     2161 tatatcccat caaaattaga aaacgtacaa tcgttgtttc ctgcaatact ggtttgggta
     2221 cctccataaa aattaaagaa atgatggaaa ataatttgtc gaaagacttg ggcattgaat
     2281 ttctgccata cgaaaatgaa actcttagag atactcagca attagagttt ctcattaaaa
     2341 caaaggatat cattgccatt atcggaacag atgatccgcg tgtgtcgaac attccctata
     2401 tttctcttga agaattattt gatggtgaaa ttggcaaact ctataaagtt cttttgagag
     2461 tctatggaaa agagattgct gaggcaatta atcaaaatat tgttaaaaat agctcattaa
     2521 accgcttgat tgacagtctt actattttag atgccgaaaa agttgtcaat caaattgagg
     2581 aaagtttaaa agattatcaa atgagggtgg ggaaaacgtt atcatcattg tccaaaatta
     2641 atctttatgt tcatatcagc tgtatgattg agcgactaat tcgtaatcag ggagtaagta
     2701 gttatccagc aatagatcag tttcttaagc gatacgaaac tgaggttgct tctattcaaa
     2761 aggcacttag taagattgag aaactttata atattaagat tcctatggaa gagattgcat
     2821 atgtgtgcaa catcattgta agttattgat aaaataaagt gacactattc acagcgatgg
     2881 aaatagtgtc actttatttt ttgtacgccc tttcatttaa gtggtgacag taattggcac
     2941 agtatttgct aataacaaag cgaaaggagt tcgggtatga gacaaatatt atgtatgagc
     3001 cacggtcctc tagcaaaagg aatgattgaa actttgagta ttatcgtagg agatttggat
     3061 aatattgatt ttcgatgtgc ctatgtcgat ggaaataatg acgtagaaat gatcatcgat
     3121 gattatctaa aggaatctat gggaaatgag acgattgttg taaccgacat ttttggtgga
     3181 agcattaata atgagtggct aagacggttg ccagatcttt ctaaagtcta tttgatttct
     3241 ggaatgaatt taagtctttt agtggattta tatttaaaat tccaattagt ttcatctgat
     3301 cagattgaag aaattattga tcatgcaatt caagcatctt ctggttcgat taagttttgt
     3361 aatcagctag attatgagca aacggttgat gacttttaaa taaaggaggg aagagaatga
     3421 ttaaattagt gaggatcgat catcgtttga tacacggtca agttgcttta acttggacga
     3481 acgcaatcag tgcggattgc attttagtag cgaacgatga aattttgaat gatccaattc
     3541 aaaagacaac tttgaaatta gccgcaccac ataacgtgaa ggttgttatc aaatcaattc
     3601 aagattcgat tgatgcaatt aatagtggga agacagataa atacaaacta tttgtagtta
     3661 ccaaaaccat ttccgatgct aaacggttgg caaatggggt tgaacaaatt aaatctgtaa
     3721 atatcggtag tgttaaggaa actagtggca gtagcgcttt gacgcataca atttttgtaa
     3781 cagaagagga acgagcagaa cttaaaggaa tggtctcaga gggcattgaa gtgttcgctc
     3841 aacaagttcc aacagataag gcaatagatt taaaaaaatc tttgtaataa aaaaataagg
     3901 gggtaatttc atggatagtc tcttagtaca agcaattttg cttggtctct tgactgcgtt
     3961 catcgtaatt gactggatga caggtacgaa taacttatca agaccaatcg tatcttccgt
     4021 ctttgttggt ttaattatgg gcgatcttaa gatggggatt atgatggggg caacactgga
     4081 gttagcattt atcggagcaa taacaatcgg agcggcacgt ccgcctgata cggtatctgg
     4141 tgggatttta gggactgcgt ttgctattgc aactggcaag ggagcagaat ttgcgttgac
     4201 cttggcattc ccaatcgctg cgctattctt attagttgat aatctactaa cattgcttgt
     4261 tctaccaatg attgctagaa aggcagatag atatgctgaa aagggcgaac taaaaaatgt
     4321 gtcgaaaatg catgttttag gttttatctt agtaaaaagt ttaccacggg ctttatttgt
     4381 cggattctca ttttacttag gaagtcctgt tatggaacga atcttaaatg ctattccaac
     4441 ttttgtccaa gatgggatta acattgctgc aggcattatg ccagcactag gttttgctat
     4501 tttattgcaa atgcttctta agaaagatgt ctttgtatat ttcgtaattg gttttgcact
     4561 ctttagctac ttacatgtgc cgattctggg gattgcaatc ttcggcgcat gtctagctta
     4621 tattatagta aacatggaaa ccaaagctgc aatgggttca agaggggagg agatggttga
     4681 agatgacttc tagtgggaaa gctttaacta aagatgattt aacaaaaatt tgtattcggt
     4741 catgtgcatt agaagcatca tggaattttg agcgccaaca acatatgggc tttgcttttg
     4801 ctatgatgcc agcgttagaa aaaatttata gtggcaatga agaagaaaag caaaaagcag
     4861 ctcaaagaca tctagagttt tttaacacag ctccgccatt atcaacattc ataatgggaa
     4921 ttgtggcttc tatggaagaa gaaaatgcca aaaatcctga tgactttgat acttctacaa
     4981 tcacttctat taaaactgca ttgatgggac cattagctgg tattggtgat tcaatgtttt
     5041 ggggtacctt attagttatc ggtatcggtg taggtgcttc attagcaaaa gaaggcaata
     5101 ttttgggtcc atttttattt ttactaatct tcaatgtccc taattttctt ctccgttact
     5161 ttggagttat gcagggatat aactttggta caaagctatt agagaacatt gaatcctctg
     5221 gcgtcatgaa aacgttgact catgctgcct caattttggg tcttatggta attgggggca
     5281 tgtctggaag tatggtatca atcaaagtag ctggttctat tggacaaggt gatgaagcaa
     5341 caaaaattca aagtatcatt gatggtattt taccaaatct gctgtcagta ttatgtgtat
     5401 ttctgattta ctggatgctt aaaaaaggct ttaaaacaac ttacgtgtta ttcggaatct
     5461 ttatctttgg tatcatagca gccttcttcg gatttttgac aacttaattg aataatctaa
     5521 ataattgtaa ggagagaact aatgttaaac tataatgcaa aagaaatgaa aaaaaaaggc
     5581 aaacggattt acggtattcg tgaagatgta gaaaaaattg caaagcaagc ttatgattct
     5641 ggaattagta atattttctt tactgcttct ggtggatcgg tagccgtaat gcaaccattt
     5701 gagtatgtat tgatgactga atcaagtatt cctgtttact ctatgactgc agctgagctt
     5761 gtcttaatgg gacataaaca gttgaatgaa aaatctttag taatcttata ctcaaaatct
     5821 ggagatacga aagaaactat taaggcagct gaatatttaa aagaacgaaa cgtgccaacc
     5881 gttggtattg taagtaaaga agatacaccg ttagaggctc tatcaactta tgcaatcaat
     5941 tacctagacg gccgccctca agaaatgcca ctttatttct taattatgtc tatcatgaat
     6001 ttgaatgggg aatttcctga atacactcgt tttgctgatg aactgaaaaa cttaccaacg
     6061 gctctgaatt cagttagaga acaggctgat gaaaaaggga agaagtatgc cgaagaattt
     6121 aaagaagatc cttatcaaat ttggattggt agcgggaatt tatggggaat cacctactct
     6181 tattcaatgt gtgtgcttga ggaatctcaa tggttgcgta caaagtctgt ctcatcacca
     6241 gagtttttcc atggtaccct cgagttagtc gaaaaaggag tttgtgtaac acttttgaaa
     6301 actgaggggg aaacacggct tttagatgct cgagttgaaa aatttgcggt ccaatataca
     6361 gataaattta atgtttttga tactaaggat tatgagctac caggtattag tgacgaattt
     6421 cgaaagtact tttgtgctcc agttatgtgg gcagccctag gtcgcgtcag tgttcatctt
     6481 gaagatattc ggaaccactc tttggataaa cgtcgttatt accgagtagt tgaatactaa
     6541 aagtaggtga ttaattatga cagcgtatgt gattggattt ggcgataatg ttgttgatta
     6601 ctatgagaat ttgaatcaaa aatttccagg gggaaatgca gttaattttg ctgtgaatgc
     6661 aaaaaagagt ggtgttaaag catactatgt cggtagtcta tcatctgatc ctgatggcgt
     6721 gttattacaa cacgccatca gttctgaagg tgttgatatc tctttctcgg aagttattga
     6781 cgagcctact gagcaagcac atgtaactat taaagatgga gatagggagt ttataggtgg
     6841 cgtgcgtggc tcccgattga cacctgctct ctcagcagaa cttattgatt tgatgaagaa
     6901 cagttcatta gttcatacta gttgccatag tcgaacagaa acaaaaatta aagaattaaa
     6961 aaaatatgat gttaaagttt cttttgattt ttctgactta gataaatata gaacgcatga
     7021 atacttgtca gatgtttgtc cgtcaattta tattgcacag ttttcagtcg ctacagatga
     7081 tcaagaggaa attaatcgct taattagtga atgcaaggac tttgaggttc cgtatcttct
     7141 ttttacgcgt ggtagtaaga gccctgtttt tgttgacact cgagagaata agacttatga
     7201 aggattcatt cgcaaagttg agtcacctca tgatacaatg ggcgcaggtg atgcatactt
     7261 tgcagctttt gcaagtaatt ttgtcttatc taaacgtgaa aaatcaatga tagaacatgt
     7321 tattgattgt tttcacaaag gtgcaaacct tgcatatgaa acattgatga ttgatggctc
     7381 ctttggtcat gctacaccta ttaaccctta gctttgtgct ttgggctttt attgctggaa
     7441 tttgtatagt agactcatca taactatatt tttgtatgtg aaaaggcaag aggctatgat
     7501 gaaaataaca ctggctaatt gcatgaggga gcatatcgtt gttaaagcgt cattgatagt
     7561 atgaataaat atctcgataa tattatattg ttatttactt aatttattga ttagtcaagc
     7621 aaagataatc ttctgttact taacgagact agtaggtaca agcacttcat gttatgtacg
     7681 acccaaaaat attgctgttg attatttgct gaaattattg cgagcttcaa tttattttta
     7741 aaggacttgg taggctgcgt gatgaaataa gacttggaag gctgtctaaa ccaatatata
     7801 gtagactaaa tagtaaaaaa gagcatggta attgtctctt tgtcaataag tctaatggta
     7861 ataagacaag ttggagattg agaatatttt tattgtgtaa cctattttga agcgtactta
     7921 actagccccg gccaaaatca aattttttcg gggtatatca agggtttgcg tcaataacag
     7981 aatgttattt atctatcacg ttccttggtg gcgtgtagag ttgatggtta cgtagtagcg
     8041 tatctaccag acgcgtaaat tttcttgcgg ttaagacgtg tgctcgtttg tgtttatttt
     8101 tgggacctct ttacatttgc tttgataaaa agctttatat tgaggaagat atttttttac
     8161 agagttggaa gcttcaacta agtagtatct aaaatagcga ctcctttgtt ttgtcagagg
     8221 tgtattttgt gaccgatagt taacagattg tttgcctttc caacataagc ctgcgtattt
     8281 tgccaatttt gtttgatctt caaatcgttc aatttgaccg atttcagcga ttaatccagc
     8341 ggcgtgtacc tttccaatac ctggaacact agtcaaatat tgatattctg gaataacgac
     8401 aacaaattgc tcggttgctt tatccaagtc tttgatgttc ttttcaagcg cacgaatttc
     8461 acatacaaaa acatttagaa cgacattgat ggattcttat gcaagactat ctaaccgata
     8521 actcacggca attgcacgtt gaatcgattt ggcatttttt tctggatctt taaagcgaca
     8581 tttccttttt cttttagtaa gtcacagaaa gcttccaaag gaagcacagc caattcatct
     8641 agcgtgaagt cttctgtcac aagagtaatg acagtggcac tatagagact tgtcgattca
     8701 tcgcgcattt atcgagtaag cgtattgcat ttgtacgtta gattttccag aaagtgttgt
     8761 tttgtacgag tgaagttgct taatgagtcg atagcgagtg cgtgttaaac gctgtaaagc
     8821 cacgtacttc tcttctttga taggagaagt tgtgaaccgt tgaatacgca ataaatcagc
     8881 aattcttaac gcgttgtttc gatcagtcgt atcttcctca aacatgcgac tgaattgttt
     8941 catccgaaaa ggattttcaa tcctcacaag tgcattgagt ttttttagct ctgcatcttc
     9001 atggaaaaac atcgaaggat gaaagctgta catggacgtt taattccatc tcaataacga
     9061 tttgaccgaa gtgattgttt tcattgaaca caagaatcag ttctcgtgtc aatggagccc
     9121 tttcaatgtc attagcaaca gaattttcag tcaatataga tagctgatcg tcgtctgtta
     9181 ggaagcaaac gtctaatttt tcagaactaa cgtcattacc aacaaataat ttcatggaat
     9241 aaaaacctat tttcgatata aattctagaa aatgcttcga tactgtggga ttccacagaa
     9301 actcactgtg tggtcacgac ctcgcctaat agaatacggt aataaaagag ctaatgagtt
     9361 gatagtaaac acagcgcttt ggataacaga cttatgaagg tagacaaagc tacaggactg
     9421 attagcaaat tctcgttgaa tcctttccat acatccattg tactctgaga aatcacacaa
     9481 taacgaaata aattccggaa ggaatatgtt attcaaaaaa atttgtgttt tatagatact
     9541 ttaaaagaaa cttattaggc aaaatatatt ttacgaagac tgatgagttg tgcaaaatca
     9601 aatctgagtc agaatgaact tcattctagc tcagattttt gtttaatgac ttcagtcagt
     9661 ttcttgcatt acgtgaatga aaatgggact ggtgatgtcc aaaccctaaa aagttagacc
     9721 aaaatctaac ttcagggggg atggtatttt tatgggaaaa tatagctttg aattcaagtc
     9781 aacaatcgtt caagagtatc ttgaaggaga aggtggatta ggatatttag caaaaataca
     9841 aggagtgaga tcaaaaaaag gtacacggat ggatcaatca aaagttagac caaaaaatct
     9901 aacttttggg ggtcgttatt tgtatggcaa aatatccttt tgaattaaaa ctaaaaatcg
     9961 ttaaagaata tcttagtggc gtgggcgaat atcggcatct tgccaacaaa tacggtattt
    10021 caagcaagtc tcaagtaagt gattgggtga atgcctatcg tgaatacgga gaggaaggtc
    10081 tattgaaaaa gcatcaaaat cagaaatatt ctgttcaatt taagttaaat gcgctagagt
    10141 tatatcaaac aagtgagatg tcctatcgag aagttgccag cactttagaa atgaacaacc
    10201 ctgcgcttat tgccaattgg atgcgtaatt tcagagaagc aggtattgag gggctctcaa
    10261 aagagaaagg gcgaccactt actttgacta gaaaaaaaga aaacaaaaaa gaaaatacaa
    10321 caactgaaga acgcgagcgt atcaaggcat tagaaaagca agtccgatcc cttcaaattg
    10381 agaatgcctt tttaaaagag ttgaggaagc tgcgaaaaca ggaagcccag caaagacgca
    10441 agaatcaatc gcacgaatca tcgccagcct ccgaggatca ttcaaactag ttgagcttct
    10501 tgaaacattg aaatttccta aagcaaccta tatgtattgg cagaaacggt ttgacagacc
    10561 aaacccagac caagaaattg aagaaaaaat gcttgaaatt cgcaaggagc ataaagacta
    10621 tggctgtctg cgactaaaaa aagaattaga gaatcaaggg attcatgtca ataaaaagaa
    10681 gattcaacgt ctgataaaaa aactaggtat cgaagtaaag tcttacactc gaaaatctcg
    10741 acggtacaat tcttatcatg ggaaggttgg cactgtcgcc aaaaatcgta ttcatcgtcg
    10801 gttttataca aatatctgtc accaaaaaat tacaactgat accacagagt ttaagtatta
    10861 tgaagtagac accacaggtg tcgttcgtca gaaaaagctg tatttagatc ctttcatgga
    10921 tttctacaat agtgaaattc tatcttatcg gatttctgaa aaaccaaacg cactagccat
    10981 aatggaagga ttagaagaag caatccagat gacaaatgat tgtccatttc gtagaacctt
    11041 ccattcagat caaggatggg cctatcaaat gaatgattat aagaataaat tgaagcagca
    11101 taaaatcttc cagagtatgt ctcgtaaagg gaattgttta gataactcac ctatggaaaa
    11161 cttctttggt ttattaaagc aagagatttt tcatggagaa gtctatcgga gtttagatga
    11221 attgaaaaca aaaattgacc aatatatcta ttattacaac cataaacgca tcaaaaagaa
    11281 attaaattgg cgaagtccgg ttcagtttcg agaagctgta aaaacagctg tctaacagtt
    11341 aattttatcg tacaaaaaag gagtggattt ctccactcca taaaagtcta actttttggg
    11401 gtcgctacaa atgcttatcg agaatttgga gaagaaggcc tcttgcgtaa gcgaaataag
    11461 cgaagttatt ctgttcaatt caagatagat gcgatagagt tataaaaaac gggtgagcta
    11521 tcctatcgtg aagtggcgaa tcatttaggg ataaaccaac ctgcgttagt tgtcaattgg
    11581 atgaaaattt ttgttcaaaa ggattgcacg ggctctcaaa aatgaaaggg tcctcctcca
    11641 ttttgtctaa gaaagaataa aaggaagaaa caagcaaact gacgcctaaa gagcataatc
    11701 gtatcaaaga attggaaaaa taagtgcgtt tccttcagat tgagaatacc tttttaaaag
    11761 aattgaggaa gcttagaaaa caggaagtct aacaaagacg aacgaatcaa tcgcacgaat
    11821 catcgacaac ctccgaggat cattcaaatt agttgagctt cttgaaacct tgaaatttcc
    11881 tgaattaacc tttatgtatt ggcaaagacg atttgatcga agaaattcag atcaagaaat
    11941 cgaagaggaa atgcttaaaa taagaagtca atataaagat tatggctatt tacgtatgac
    12001 aaaggaattg tgggaccgtg ggttccatgt taacaagaaa aaagtacaac gtttgattcg
    12061 aaagctgagt attgaagtac gatcatttac gagaaaatat cgccgctata atacttatcg
    12121 tggaagaatc ggcatgatta caaaaaatcg aattcatcga cgcttttaca caagcaactg
    12181 tcatcaaaag gttacaacag atacgacaga actaaaatat tttgaaatgg ataaatacgg
    12241 tgccaatcgt gaaaagaagc tgtatcttga tccattttta gatatgtaca acagtgaaat
    12301 tttattttat cgcatttctg aaaagccaag tgcgttggct atcatggaag ctttagagga
    12361 agctatctgc gcaacggaat gtatctatcg tcgaactttt cactccaaaa aggatggacc
    12421 taccaaatga aagcttatag tcataagttg aaagaacata agatcttcca aagtatgtcc
    12481 cgtaaggaga cctgcttaga taattcgatc attgagaact tccttagtct attaaagcag
    12541 gaaatttatt atggaagaga ctatcgtatc tttgaagaat tgaagcaagc cattgatggc
    12601 tatattcatt actacaacca tgagcgtatg aaaaggaaac taaactggca gagtctggtg
    12661 caatttagag taatagcatt caaagctgtc taaaatcatc aagaaaagca aagaaaaacg
    12721 gagtgaattt tctccactcc gaaaacatct atctttttta ggtcactaca gaatcaattc
    12781 tgattcatca gacccataaa ttatagatcc taataattta taccatgctc tttttactca
    12841 tatttacatt tatctttggg tattttatgg tttaag
//
LOCUS       DADXTH010000078        12800 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.078, whole genome shotgun sequence.
ACCESSION   DADXTH010000078 DADXTH010000000
VERSION     DADXTH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.078"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1183)
                     /locus_tag="IWI97_002248"
     CDS             complement(<1..1183)
                     /locus_tag="IWI97_002248"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBC2463736.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
     gene            complement(1362..2227)
                     /locus_tag="IWI97_002249"
                     /pseudo
     CDS             complement(1362..2227)
                     /locus_tag="IWI97_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2209..3723
                     /locus_tag="IWI97_002250"
     CDS             2209..3723
                     /locus_tag="IWI97_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBC2463737.1"
                     /translation="MFSHEERVKAIQLFLKYDCSYAATIRELGYPSVGALRKWYNEYL
                     ISGALHLEHRKKSKYSEEQKRVAVNHYFEYGQCYARTIRLLGYPNRESLRHWCEELAP
                     GARKLRKSAVKLTQDQKDNVLKKFYAPQANRKSLAEAEGISRVTLYQWKDMYLGRGFP
                     LRMTQENQEEQKELLLTEVKELQKQVHQLQLEKALLEGATELLKKEEGINLLQLTNQE
                     KTRLIDALRNQFKLNELLQQLYLSKSSYFYQKHVIDKPDKYAEERQLIVTIFNSNFCS
                     YGYRRIHQELKNMGKILSEKVVRMLMIEENLIVTFSKRKKYSSYAGEISTAQPNLLNR
                     NFKATRPNEKWLTDITEFKIPAGKVYLSPIVDCYDGAIISWTIGTKPDAALVNTMLDI
                     GLATLQNQERPIVHSDRGAHYRWPGWIERMNKANLPRSMSKKGCSPDNSACEGFFGRL
                     KNEMFYQRDWKNTSMNQFMDHLDDYIQWYNNHRIKLSLGGLSPLQYRKKQECNC"
     gene            complement(4027..4869)
                     /locus_tag="IWI97_002251"
     CDS             complement(4027..4869)
                     /locus_tag="IWI97_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBC2463738.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFTILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            5194..5610
                     /locus_tag="IWI97_002252"
     CDS             5194..5610
                     /locus_tag="IWI97_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBC2463739.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            5607..6077
                     /locus_tag="IWI97_002253"
     CDS             5607..6077
                     /locus_tag="IWI97_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2463740.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            6091..6864
                     /locus_tag="IWI97_002254"
     CDS             6091..6864
                     /locus_tag="IWI97_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2463741.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            6878..7690
                     /locus_tag="IWI97_002255"
     CDS             6878..7690
                     /locus_tag="IWI97_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBC2463742.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            7744..8709
                     /locus_tag="IWI97_002256"
     CDS             7744..8709
                     /locus_tag="IWI97_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2463743.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTANEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            8846..11569
                     /locus_tag="IWI97_002257"
     CDS             8846..11569
                     /locus_tag="IWI97_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2463744.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDMLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSERLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            11582..12214
                     /locus_tag="IWI97_002258"
     CDS             11582..12214
                     /locus_tag="IWI97_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBC2463745.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
BASE COUNT         4306 a         2055 c         2497 g         3942 t
ORIGIN      
        1 tatggagatt ttccagtcgt gcattcgaat acttcttcag taatgccagc cgaatccctt
       61 tctcgtattt gaaaaaggct tttttgatat ggtgcagttc caatggaact tctccaggca
      121 ttgctcgaag cagctggaaa aaagtatctg gatctttttc ctgatacgca gtaagcaggt
      181 cttggtagta acgataagct gttttaagtt caggagaaat ctttagaagg cggtcaacta
      241 cttctgtttc agtcaatagt ctccaattga aattgcgtct tttcgttagt gtattgtaat
      301 ctaaacgatc ctctgatttt aaaagtaatt tgaataagga tttcaattgg cgatactgtt
      361 tagcttgtat tgggtctttt tgatcaagtg ttttcatcac acgttttcga accgcatcga
      421 agaagcggtt cagatgttga acaatgtgaa accgatcgac gatcacttgt gcatttggaa
      481 aacactcttt aactagtgag aaataaggag cgttcatatc cgttacgatg taatgtacac
      541 ccaacctcgc tttgagagaa aaacgcatga aaaaagcaac gagatctttt ttacggcggc
      601 taggtaagat ctcaaacagt ttcccggtct caccgtccat acaagaaaaa gaaaatttac
      661 cgtgccatgt agaaagagaa cgaaactcat cgaataatag acaccgaggt aaatactcaa
      721 gattctgacg gatatctgat gacaaggcgg acaatgtccg ctgaacagtt gtcacagaaa
      781 catcgcaatg tttaccaatt gcttttatag attgacgctc tgtcaattct tctaagatct
      841 tgtaatgaat ctgcttagaa ataaagcaga atggggtaac gagatacgtc cgagcggtaa
      901 aatatttttt acatgctcgg cagaggtatc gctgtttctt caaccgaaga taggtccgtt
      961 tattaccgct acgattcagg agaatcatag agatcttctt gccattttta acgatttgct
     1021 ttgtagaatc tttacaacac ggacaacgtc tgtaggtacg gatcagagtt ccctcaataa
     1081 cgatactttt tctgctttta taaaaagttt cttcaaagga ttgaatttgg atatttgagt
     1141 ctttaatagt aaagagttgt ttgataaaat gattcatgga catacaagaa ttcccccaga
     1201 atgatgttgt aggagactct attctaccgg aaattttgta tgtctttttg tttttttaca
     1261 aaaaaactag gacagataga aaatttccat cagtcctaga aattatagag ccaataaaac
     1321 ttagtccgat atgcggcgcg ttctgtgcgc cgcatatttt tttagttttc aaagatcaga
     1381 ccttgttgta aatagatccg tgcccgaaag ttataaaagt tgcgataacc aaacgcaatt
     1441 cgtttaatca gcttgatctt gttatacagc ccctcagtga cgccgtttga gtgggacacc
     1501 ttaaaggcat ttgaaatacc cgattggtag cgatggaaga tcgtgaattt ctttttaaac
     1561 gactgaggta acgtagatga aattgtttta agggtttctt gaaagcctac cgtatctcgt
     1621 ttaacgaaat aatagcgtaa taattgacaa aaatgatacg cagttcgcaa ttcttcgttg
     1681 tagcttaaca gctcattaac gatttctgtt tgactaattg gtcgtttaaa cagtcgatga
     1741 taatgccgtt tgagtgcctc ccatgtcgtg gaatctttga gtaagagttt ccaatatcgc
     1801 ttgagccgac ggtacttctt ctgattttca gagtgtgagg ttcgaaaacg attcatgatc
     1861 tggacacgta attgattgaa gctgcgattg atgtgttgga caatatgaaa gcggtcagtt
     1921 acgatctccg catttggaaa gaccgtttta agcaactgac aatagctggc attcatgtcc
     1981 atcactaggt acttgacctt taaacgggcc tttctaggaa agcgcaggaa gtattgagtt
     2041 agtttgtaaa gtctgcgatc ttcaagggtc gtaaatagct gattggttgt cccatcaaca
     2101 caaataaaac tcatggaacc agaagtggca acattgtgta cttttctttg caatctgggg
     2161 gtgcggacat aatcctggac taaaaatgaa cacaggagga atgttcgtat gttttcccac
     2221 gaagaacgtg taaaagccat tcagttattt ttaaagtacg attgctccta tgccgctact
     2281 attcgtgaat taggctatcc gtccgttggc gcacttcgca aatggtacaa tgaatatttg
     2341 atatctggag cgcttcatct agaacaccga aaaaaatcaa agtattctga agaacaaaaa
     2401 cgagtagctg tgaatcatta ttttgaatat ggacagtgct atgcaaggac gattcgattg
     2461 cttggttacc ctaatcgaga gtctttacgg cactggtgtg aagaactagc gcctggggct
     2521 agaaaacttc ggaaaagtgc agtaaagtta acccaagatc aaaaagataa cgtgttgaaa
     2581 aaattctatg cgcctcaggc caacagaaaa agcttagctg aagccgaagg catcagtaga
     2641 gtcactttgt atcaatggaa agacatgtat cttggaaggg gttttccact gcgtatgact
     2701 caagaaaatc aagaagaaca aaaagagtta ctgttaacgg aagttaaaga attgcaaaaa
     2761 caagtacacc aattgcaact cgagaaagca ttacttgaag gagctactga actgctaaaa
     2821 aaagaagaag gcatcaatct ccttcaactg acaaatcagg aaaagacacg attgattgat
     2881 gccttaagaa atcaatttaa attaaatgaa cttcttcaac agctttattt gtctaaaagc
     2941 agctattttt atcagaaaca tgtaattgat aaaccagata aatatgctga agaacgtcaa
     3001 ttgatcgtga ccattttcaa cagtaacttc tgctcttatg gttaccgcag aattcatcaa
     3061 gaactaaaga atatggggaa aattctttct gaaaaagttg tacggatgtt gatgatcgag
     3121 gaaaatctaa tcgttacatt ttccaaacga aaaaaatact cttcgtatgc tggtgagatc
     3181 agtactgcac aaccaaactt attgaatcgt aattttaaag cgacaaggcc taatgaaaaa
     3241 tggttgactg atattactga attcaaaatc ccggcaggaa aagtttattt gtcgccaatt
     3301 gttgattgtt atgatggagc tattattagt tggacaattg gcaccaagcc tgatgcagca
     3361 ttagtgaata ctatgttaga tattggacta gctacacttc aaaaccaaga acggccaatt
     3421 gttcattctg atcgcggggc tcattataga tggcctggtt ggattgaaag gatgaacaaa
     3481 gcaaatttac cacgttccat gtcaaaaaaa ggttgctcac cagataactc tgcgtgtgaa
     3541 ggcttctttg ggcgattaaa gaatgagatg ttttaccaac gggactggaa aaacacctca
     3601 atgaatcaat ttatggatca tttggatgac tacatccagt ggtataataa tcatcgaatc
     3661 aagttatctt taggaggact aagtcctctt caatatcgga agaaacagga atgtaattgc
     3721 tgaaaaaggt ccaaaaaaac atccgcaccc ccttgtatgt acttttattc tgccataaac
     3781 agcttaatga atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa
     3841 ggaagtaaac ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt
     3901 tagtgaaaaa acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc
     3961 cagcaaacat tagaggggat atttcatata gcttttgtcc tctcgacagt cctatcagtt
     4021 tctcttttaa cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa
     4081 ctgtgttttt aatatggtga aggcttttga acctgtgcag tggcaagtaa ggattttttc
     4141 tactcgtgag tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa
     4201 attatttata taaggtatgc caatcatatg gaagccaccg attaggtatt ttatgggttt
     4261 agtcggaaat gtttcaagag caatttccat catatcgata atgccatgat gactacagcc
     4321 agtaaagaca atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc
     4381 atcataaaca aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt
     4441 gcctaggggg atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa
     4501 attctcatgc attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa
     4561 aatctttaaa taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga
     4621 ttttaaatat cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc
     4681 cagatcattc aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc
     4741 agcatcgaag agatatttct tccctgagta gttaattaag agagataacc catgtccact
     4801 tttaaagttc aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc
     4861 tcctttcaca tgaacgtgta ttcatatact gtagcacatt aagttctatt ttttgttgta
     4921 attaccgaaa aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat
     4981 ttcttaggat gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa
     5041 ttagacacct ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt
     5101 tggcatgata tagaaaaacc tctaattgac agcgtttcct aaaattggca cgcatcttgc
     5161 ttatagaaaa gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat
     5221 ggggaatttg ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca
     5281 attcatgcct tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa
     5341 cttttagcca caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt
     5401 gtgaataatg atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga
     5461 atgaacttac ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag
     5521 ctagaaaagc ttattagaga gagtcaacaa tcagtgattg attgtagaaa attattagta
     5581 gaacaactta ctggaggaga tgacctatga ttaaattagt acgtattgac catcgtttgt
     5641 tgcacggaca ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg
     5701 ccaataaaga ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga
     5761 ctggcattaa attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg
     5821 aaacacttaa cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt
     5881 gtgaagccta tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt
     5941 ccaagcaatt tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat
     6001 taaaagctct tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt
     6061 tgaatacaat gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta
     6121 atcggtgttt tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg
     6181 ttgattgtca gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt
     6241 ggagcctctt tggaattgat gtccttagga ttagtaaata tcggagctgc tgcaccacct
     6301 gatatgaata tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca
     6361 gaagcagcat taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatcttg
     6421 atgcggacga ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaagggaaa
     6481 tttagccaag caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat
     6541 tttattccta tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat
     6601 gcgattcctg agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat
     6661 gggtttgcgt tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt
     6721 ggatttttaa ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt
     6781 attgttgcgt ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag
     6841 ccagatccat tagatgactt ataaggaggt aaattaagtg aaagagaaag atcaaaatgc
     6901 atggaaatat cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga
     6961 acgacaaatg aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa
     7021 gaatccagaa gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa
     7081 ctgtacccct caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata
     7141 ctacgaagac caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat
     7201 gggaccgcta tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt
     7261 tggactagga attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt
     7321 aatttccttt gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat
     7381 ggggaataaa tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt
     7441 ttgctccatt gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac
     7501 aacaccaatt caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc
     7561 acaattttta ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa
     7621 aacaggatgg atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat
     7681 cttacaataa gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa
     7741 ttgatgaatt caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc
     7801 gtatttgttg gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac
     7861 caaaatgcga agcagttacg gattagtcat tacacagcca acgaatttaa ctatgcaaca
     7921 ccagcatctg tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca
     7981 gaaacagttg aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca
     8041 cgaagcattg actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca
     8101 gagaactatg ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt
     8161 ttacatcaag tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc
     8221 tatgatcttg cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa
     8281 cgctttaaag atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca
     8341 tactcaacat caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat
     8401 tcaggagaat ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta
     8461 ttaatgaatg atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc
     8521 tttgatacga aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct
     8581 aaagaagtct tagactactt taacccattc gtgattactg cagtatttag agtttatgca
     8641 gaagaattgt cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta
     8701 acttattaaa gaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt
     8761 tcttcaatgt gattttatac atatggtaaa ataactagta catatggtaa aataactagt
     8821 acatattgtg aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac
     8881 attgcttttg aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagcttt
     8941 acagttgaat agaagtacaa tcagtagcta cttaaatgaa ggtgttcgcg aagggctttt
     9001 cataaaagta aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca
     9061 tatcacactg aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa
     9121 acccgcatta gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat
     9181 taaaacggca gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg
     9241 atcagggaaa acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat
     9301 tcacacatcg gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt
     9361 actatcctct gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca
     9421 tactggtttg ttagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt
     9481 atctgaagaa gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat
     9541 gggggataat agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa
     9601 tatatatacg actttcttac caacttttct taggagattg ccagttattg ttaatctacc
     9661 tagatttcaa caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc
     9721 tgagagtaaa attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat
     9781 gaatagtgat tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg
     9841 aaacgcatat atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc
     9901 tttagagtac agttataaat tcaaagagca actaaataaa cccagaaaga aaagagcaga
     9961 tcgaatctac tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt
    10021 tgttcaattt tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga
    10081 tattaaacta tttttagaag aaatggttac caaggtaaca caagtaatgg atatgcttat
    10141 ttttcaagag gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg
    10201 tcaaagtatt gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt
    10261 cgctttggcc aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca
    10321 agaaaagaaa accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta
    10381 ttggtatgca aaaaaaatct taagcctttt atctgagcgt ttagatcaac ctgtgtatga
    10441 agaggacatt ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac
    10501 aggtattaag agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt
    10561 tgccaaccgt atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac
    10621 attagagaaa attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg
    10681 cttgatttta ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca
    10741 tctaaatgga ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg
    10801 ccaacaagta ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct
    10861 tactttcaaa aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg
    10921 ttgttataca gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa
    10981 agatgcagtt agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa
    11041 gaattttgag actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc
    11101 caaaattaat cagattccat atattggatt agatcgcctg attaatgggg aagcaattgg
    11161 cgaatttgtt cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga
    11221 attaattttc aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga
    11281 taaggtgcta tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga
    11341 tttttctaat aatcagctgt ttttgttgta ccttcactgt agttgcatga ttgaacgaat
    11401 tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg
    11461 agaaataatg gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga
    11521 aataccagat ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg
    11581 aatgacaatg ggagtaattt ttgatatgga tggggtactc atagacagcg agacatttta
    11641 ttttgagaga agaatgaact tctttcaaga actggagata gatccaggat caaaccagat
    11701 tgatgattat gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa
    11761 atcattaaga gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga
    11821 tttcgctaaa gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga
    11881 tataccattg gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca
    11941 atgtgaactt actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa
    12001 accacatccc gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc
    12061 agtagaggat tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggtggc
    12121 actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca
    12181 agaactggaa caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata
    12241 tctgtgctag ctattttgac taaccaaaaa aactgttgcc attccacaac tttattttca
    12301 gatttagata attcttgcaa atatctattg tacttaatcg atcaactatc cataacttta
    12361 tctttgagtc gtaaataaac ataagattat tgatatggaa gaaattcaaa aagatatgaa
    12421 actaatcaga taggtaagaa atatcttaag ttgtaataaa gagatacaat aacccttaat
    12481 caacaattac taaagagctt aaaaaaatac gtgaatcatt tagaaaaaaa tcaattatct
    12541 tcaattatag taaatgaact taacttcata gctgaaacag aataacttga atcctcaaat
    12601 aacttctcta cctgctgttt tggttacgat attggctacg ttcccgatga taatatctgc
    12661 tgctatatac tgtcagtcta tagtaatttg ttaaatgaaa tatctataca aagcttattc
    12721 ctttactctg attcccaaaa gagtcattgg aagggtctgt ggggtaatac ttaaataaag
    12781 gctaggaacc ttgatgttat
//
LOCUS       DADXTH010000079        12610 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.079, whole genome shotgun sequence.
ACCESSION   DADXTH010000079 DADXTH010000000
VERSION     DADXTH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.079"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            159..965
                     /locus_tag="IWI97_002259"
     CDS             159..965
                     /locus_tag="IWI97_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2463746.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            977..2197
                     /locus_tag="IWI97_002260"
     CDS             977..2197
                     /locus_tag="IWI97_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463747.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2187..3773
                     /locus_tag="IWI97_002261"
     CDS             2187..3773
                     /locus_tag="IWI97_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2463748.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3812..5950
                     /locus_tag="IWI97_002262"
     CDS             3812..5950
                     /locus_tag="IWI97_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2463749.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(6318..7301)
                     /locus_tag="IWI97_002263"
     CDS             complement(6318..7301)
                     /locus_tag="IWI97_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBC2463750.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            7679..9070
                     /locus_tag="IWI97_002264"
     CDS             7679..9070
                     /locus_tag="IWI97_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBC2463751.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            9086..10126
                     /locus_tag="IWI97_002265"
     CDS             9086..10126
                     /locus_tag="IWI97_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2463752.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRKIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            10145..11230
                     /locus_tag="IWI97_002266"
     CDS             10145..11230
                     /locus_tag="IWI97_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBC2463753.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            11263..12225
                     /locus_tag="IWI97_002267"
     CDS             11263..12225
                     /locus_tag="IWI97_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBC2463754.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            12218..>12610
                     /locus_tag="IWI97_002268"
     CDS             12218..>12610
                     /locus_tag="IWI97_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HBC2463755.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVF"
BASE COUNT         4355 a         1925 c         2400 g         3930 t
ORIGIN      
        1 ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat
       61 agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact
      121 tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat
      181 ttatgaagga catttatcag aacagaaaat tactgctaca gttttcgatc aatgacttca
      241 aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta
      301 ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa
      361 aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg
      421 catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat
      481 tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct
      541 tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt
      601 tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga
      661 cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt
      721 tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa
      781 gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa
      841 gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag
      901 taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc
      961 tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca caaaatctta
     1021 taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc
     1081 ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat
     1141 gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg
     1201 agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga
     1261 actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat
     1321 ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc
     1381 tgacatcggc gaccatattt atcaacctgt aaaaacctat tcaagtggga tgttcgttcg
     1441 tctagctttt gctgtagcga tcaatgtgaa tcctgatatt ttgattgtgg atgaagcatt
     1501 ggcagtagga gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa
     1561 ctcgggaaag acgattttat tcgtttctca tgatattagt tcgattcgcc gtttttgtga
     1621 tcgttcctat tggttgaaaa atggaaaagt tgtcgaatat ggtgatacga tggaagtaac
     1681 gacgaattac gaaaactttt tgaagaaaaa atcagttaaa acagtagatc gaaataaaaa
     1741 ccaagtagaa cgcttcgcag ctccagatat cgtagacgtg acgaaagccg aattgttgga
     1801 taaagataaa aatccagtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac
     1861 ctatgaagta aaagacgata caatcaaaaa tccagtttta ggaattgctc tgcgtacagt
     1921 cgacaatttt tatgtgtgcg gactcaatac tctactagat ggtatcaaga tcccttggaa
     1981 aaaaggtgag aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata
     2041 ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat acaagacaaa
     2101 atacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca
     2161 taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac
     2221 aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg
     2281 gtcctggaaa cggccgatta acgaaacatt tgatcggagc aaaacaatgt gaagtgagca
     2341 ttgtcgagct agataaggaa ttgttcgatt ttgtcagcga gtttgcccaa gatggatttt
     2401 atggagatat cgaaagtttt gaatgggcaa actattatgc tggacaaaca tttgattacg
     2461 ttttgtttgc cgatgtttta gaacatttgg tagatcctgg aaaaacgttg aagaaagtta
     2521 gagaattttt gaatgaagaa ggagaaattc taattacatt ccctaacctt gcgcataatt
     2581 ctgtcatgat tgatttgttc aataatcaat tgccatgggc ttcttatggt ttgctagatg
     2641 aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcaggat
     2701 tatacatcaa tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa
     2761 cttatgaaga attgccggaa gctgtacgtt atgatttcaa aatgcgacca tttggcgaag
     2821 tctaccaata cttcttctca ttgatgaaac atccagtagc acaaagttcc attgcgcagc
     2881 cgcaaaattc taattatgtg aaagtattag aagtaatcca gcaaacgaaa caagatgaaa
     2941 taattcaaca aataccattc aataatttca caggggaaaa tgagacacta agtttccatg
     3001 tatcagaatt agcagaacga atggtgtttc gcttcgcgca gcaacctagc tttatcgaat
     3061 tcagcgcaga agctgctgga gaaaaactca catttatcga ttcaaatgct gtagtcaaaa
     3121 cagtaaatga ttgttatttg tttgatggta aagatttacc agagttcttt ttaacggata
     3181 ttgcagggaa agaagtgact atccattgtc actatcgttt tatcggagaa ttgacaccaa
     3241 caatgaaaga attgctggaa acaatccgtc caatggctga agttacaaag cagctttctg
     3301 agaaaaatga tgaattagaa agagaaaatc attatcttat tgagaataat acaagattag
     3361 atcagacgtt gaaaacaacc ataaatcgct attgcacgtt gttggaaagt gatgaatgga
     3421 caatcaaaca tagattaggg cgacgtaaga aagaaacatc gaaaaaaatc caagaaaaag
     3481 agctttctct atgtattgac gaaaaaatct gggatgcaga aacaaagata ttgaaaatca
     3541 tcgggtgggg aatcgctaat tcggatcgcc aaccattgtc ctataagtta tcagccgacc
     3601 aatcaccatt ttttgaagca attcctgttt tacgagaaga agtcaatcaa gcaaaacagc
     3661 tagctgaagg agcaaaagca ggatttgaac tgcggatcct ttgcgagaag gaacgttcat
     3721 tcttagttga agcagtggct caaaatggtc aatcatggat cattgaaatg taaataatga
     3781 ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat
     3841 cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga
     3901 tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc
     3961 aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag
     4021 cggttttaag gtacgtttat cagggaaaat gagaggaaaa gcaatcttag acttccaaac
     4081 agcaagacat caaaatggca tagctgtgaa attaaacggt aaatatcctt atgatgacgg
     4141 cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag
     4201 gactcatggc gtaaaaaaag caatccgccg ggtcaaactg gaattgaaac caggatcgat
     4261 cgactatgca gagtggatca gtcgtcatga gaaacctgat ctgaaagagc aaaggaaaga
     4321 agtacaagga tttgcctacc gcccattgat ttccatactt atgcctgtct ataacgttga
     4381 aatcaaatgg ctcgaaaaat gcattgattc ggtacttgat caaacgtatg atcactggga
     4441 actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc tagaatccta
     4501 tcaagcaaaa gatgatagaa ttaaagtcgt tttccgacaa gaaaatggac acatcagcct
     4561 ggcaactaat tctgcattgg aaatggcaga aggagagttt atagcgctct tagataatga
     4621 tgacgaatta cctccatttg ccttatatga agtggcaaaa gtgctaaacg tacatccaga
     4681 actggatttg atctacagtg acgaagataa aatcgatgca gatgggaatc gctttgaccc
     4741 gcacttcaaa gcagactggt ctcccgatac attgatggga aataattata tctctcactt
     4801 aggcgtatat cggacaagca ttgttaagga acttggcggt ttccgtaaag gatatgaagg
     4861 ctctcaagat tatgatttgg ttcttcgtgt gacagaacaa attcctgcag aacacattta
     4921 ccacattgac cgtgtcttgt atcattggcg gacgatcccg ggatcaactg caagcaatgg
     4981 agaagctaaa agctacatct atgattctgg agtgaaagca ttgacagatg ctctatctag
     5041 aagaaatatc aagggaagcg tacatcctgg cagaatttct ggtttttatg aaatcactta
     5101 tgatgttttg caagaagact tggtcagtgt gatcattcca actaagaatg gctatgaaga
     5161 tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tatccaaatt atgaaatcat
     5221 tattgcagat aatggaagta cagatcctaa aatgcaagaa ttatttgcag aatacaagca
     5281 tcagctgaaa gaccgattta tcgtcgaatt aattgacatt ccattcaatt attcacgtat
     5341 caacaatctg gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac
     5401 agaagtgatt gaacctgact ggatgacagc gatggtttct tatgcacagt ttgatcgaat
     5461 cggctgtgta ggagcaaaac tgttttatcc agatgataca acgcagcatg caggagtatt
     5521 attaggaatc ggtggtgttg cagggcatgc attgaataac tatgatcgaa cacattgtgg
     5581 ttatttcggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat
     5641 ggtcaaggca gctgacttca atgctgttaa cgggtttgat gaaacattag aagtagcatt
     5701 caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca
     5761 tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata ctccagaaaa
     5821 acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat aaatggccag catatattgc
     5881 gcatgatcca ttttacaatg ataatttaac aaaagaagga atcggcgact tttcgttgag
     5941 accagattaa actataataa aaagctgtta tgttgtgatt cgctcgcaca taacggcttt
     6001 ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa gtcgaggttg
     6061 ggacagaagt gtttaactcc gagaaataag aagaaattca cgaaaattgc ttttcaaatt
     6121 tttgtgaatt tcggcttatt taagaagaag ttgcttctgc tcccaccgtt tattcgtttt
     6181 taaagaatga gacaaaagtg tttcttactt ttgtcccacg ctcttattca tgctcagagc
     6241 gccaatagat acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa agaatcgata
     6301 ttttatttta atataggcta ttttaaaata aatggtttaa ggtattcctc tacaaaagcc
     6361 actctatcaa ttatcatggg tttaaaataa gaagtgatgg agataaccag attgttctta
     6421 gcgtcaatat aaagaacatt tccactatta ccaattgctg aatatatttc gttctcagaa
     6481 tctaataccc accataaata gccatatctc atattttgta agtcttctaa agcactggtt
     6541 ctaggtttga acatctcgtt tatccacatg ggtggtacga tttgttttcc tttatacatc
     6601 cctttattta ggcataatac accaattttt gccagatcac ttgccgataa acacaaacca
     6661 tatcctgccg tcccgatatt tagtggatca caaaaccaga cattggtttt aggagactta
     6721 tccattgtaa actgcttatg ttcttcagct gtttcagcat aataattgac atggtttggt
     6781 atccctatag gatcaaatag atacttattg gcaaaatcaa cagttgttag cttacttgtc
     6841 tgatacaaca agccagttaa tatgtgtaaa cagacagttt gatagttgaa ttcgccggtt
     6901 ataccttttc ttcctcccaa taaatctagt gatgtttttg tccaatcttc acttgaacag
     6961 acttttgtcc atggatcgcc tttacatttg tagggtgctt tcattgttag caagtgcttg
     7021 attgtaatag cgtaaatagt tttctccccg cgtttcactt tatactctgg aaaataatct
     7081 aatactttat catcgatact tttgattaat ccttgctcta aagcaatgcc aatcagcaaa
     7141 gatacaatac ttttcgttgc tgacatgata tgacaagtat cgtctttttt ataaccattc
     7201 cattcatctg tataaacttc taccccatcc ttatatgcga caatttgaca tatatttggt
     7261 tgtcgaaaac caataaaatc atgcaactct tgtttattca taagatatta cctcactttc
     7321 ttctataagc ataatgtcat taaaaatcga tttcaatttt ttcgattctt cacgtaatac
     7381 aggtattctt atggactaga cagcgctttg ggaagatgga ttctctgaaa aatataaagg
     7441 ttcactttat gagtggataa aaaaagcagc aaatacttcg acttctttaa tttaccaaca
     7501 ttagtgaaaa tcgattcatc tggagatttg taatttagag tccttgtctt gattagaggt
     7561 gtataaagct ttttattaga catttttaaa ttccattaca atttgcttat aaactaggtt
     7621 tttaaaggtg actatggtaa gataatagag atattatttt gtgattagga gtgtatatat
     7681 gaataaaaat ggagaatgga gtgctgcacg acggatattc attatattaa tggatgttat
     7741 tgtatacaat gcagcaatat atagtagttt cttactgaag tttcgtggag agatcccatt
     7801 acgaaattta gaaactttcc aacactcagc aattattatt tcagtgattt ttattgcgtt
     7861 gaacattttg ttgggtgcat atgtttttta taatcgaatg atcagtgaca tcgtgtttgt
     7921 cacagtcatc ggtcaggtat tgatgacact agggatcatg gtagtgactt ttgcgggccg
     7981 ctggtttgct tttccacgtc ttgttatctt actttcattt gttctcggta cgcttttctt
     8041 aagtatctat cgtattctcg tatataaact ttatttacgc gtaagtcgag ataaaaaggt
     8101 cgtcattatt gggttagaaa aggatgtcgc accagcaatc caaaattttg gttcaaagaa
     8161 aaacaacaaa cataaagtcg aagcagtcat tcttgataac tattatgcaa acatcaaaaa
     8221 tatgattaaa gacatcgata ttgtctatct tgcttctcat atcgaagaaa ctgaaaaaat
     8281 caaaatctat caattagtta ctaaaaatga aaaaaaattg tttttgaata caacatttga
     8341 aaacttgaca ttgatcaatc caaatattat gaatttcgaa gatgaaagta ttattgaagc
     8401 atctggattt agaatccaac ctgaatatga actattcaaa cgtttgtttg atatcatcgt
     8461 ttcacttgtt ttattagtta ttgcttcacc atttatgtta ttcacagcaa ttttagtcaa
     8521 agtgacatca ccgggaccaa tcatctataa acaagtgcgg atcacaaaaa atcaaaagga
     8581 attttcaatc tataaattcc gctcgatgac cgccacagcc gaagcaaaat ctggaccagt
     8641 tttagctaag agcaatgatg cacgagtaac gcctgtaggt aaatttattc gagctgtccg
     8701 ctttgatgaa ttaccacaaa tcttcaatgt gctgaaaggc gatatgtcga tcgtaggacc
     8761 acgtcctgaa cgtcctttct tcgtcgatca gttcaatgca gaaaaccctt attactactt
     8821 acggcataat gttcgtgctg gtatcactgg gtatgcacaa gtctatggaa aatatgtctc
     8881 tgattacaac agtaaattac gttttgattt actttatatc aaaaaatatt cattgatgct
     8941 tgatttaaag atcttgctgc aaaccatcaa gatcctattt gataaaatgt cctctcaagg
     9001 acttgaagaa gacgaaaatg tgatctcatt ggcagaaatt gcttttccga atgaaaaaat
     9061 ctttcgctag ttgggaggcg taaaaatgaa caatccgaaa ttcagcataa tcgtaccagt
     9121 atacaatgca gaagcaacta ttggtcgaaa gattgatatt ctaaaagaat tggattatga
     9181 agagtacgaa gtcgttttag taaatgatgg atcaacagat caaacacagg aaatcattca
     9241 agaactgatt gaaaatgatg aacgttttga attgattact acgaccaatc aagggcccgg
     9301 attagcaaga aacgaaggaa taagacagtc aaaaggagaa tatctcttat tcttcgatgc
     9361 agatgacacg ccagtaaaaa cgatcttgtc agattatgat catttgctag aaaagagtcc
     9421 aaaagcggat cttgttgtta gttcgtttgt ttttcgtaca atggacgagg gaagaatcgt
     9481 ttccgaaaaa gaaaacttag ttacagaatg tcactatcta actcacgaag agtttataaa
     9541 agacatgtat cagttaatga atgaacagtt gatgtatgtt gtatggaaca aatgctatcg
     9601 tagcgatatt ctgaaaaata accaaattct atttaaaact tataatagtt gtgaggatcg
     9661 aatatttaac ttacattatt atgaatactg tcatcaagtg atcatgaacc caaaagtaga
     9721 atatatttat gaatttgaag gtgggaaggg gatcactaat caataccgac ctaacaagtt
     9781 cgaaacattt aaagaattct attcattagc aaatactgta acagacgata ttaataaaga
     9841 cggtatggcg gcgcttcttt taaaagggac aacttcagtt attttttcta tttatagtac
     9901 ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag cagatattga gtgatccagt
     9961 gatccgagaa gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa taacaaagtt
    10021 gctttacaat atgccagctc cactattttt atctgctgta aaagttggaa gcttcgttga
    10081 agtgaaaatg ccagggttaa tggctgcttt gaagcgagta tattagttaa tggaggaaaa
    10141 tataatgaaa agaattttgc attttcaggg acgaatgggt ttaggtggag cagagtcttt
    10201 catgatgaat ttatatcgta aaattgatcg tacaaactat caatttgatt ttttgattta
    10261 tgaagattat gcagatgttc aagactatca ttcagagatt gaaagattag gaggccgtat
    10321 ttttgttgta cctaatccta agaaaaatat ttttaaatat ttaatagaag tgaataagtt
    10381 actaaaaaaa gaatcttttt ctatagttca caatcaggtt tattttggcg ggggaattaa
    10441 tttatggtta gccaaaaaga atggaatcag acagagaatt gctcatagcc atgcaactga
    10501 agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt aaatttttaa ccaaattgtt
    10561 gttacaaaat gcaacagact accttgccgt tagtcaagaa gctggtgaaa gtctgtttca
    10621 aaaccatccc tttgagatag ttcataatgg tattgattta gagctttata gtaaaaattc
    10681 agaagctaag gttaataaga gaaaagaatt ggatatctca atgagtactt ttgttgtggg
    10741 aaacattgga cgattagaga aacaaaaaaa tcaatcttat ttgttagaga tttttttaga
    10801 aatactaaaa atgaattctg attcttactt gattttagta ggtgaagggt cacttagaaa
    10861 taaactgaaa gccaaagcag tagaattgga tatagagaaa agagtaaaat ttttaggaga
    10921 aagaaacgat attcccgagt tattatctgt gatggatgtt tttgtaatgc cttctcttta
    10981 tgaaggtctg ccaatggttg gattagaagc acaagcagca aaattaaaat tagtcttagc
    11041 agatactatt tccaaagata caaagttaac aaataatgtg gattttgtat cattagatga
    11101 aacaccacaa gaatgggcaa aaaaagtaat aaaagagccg tatgctaata aaataacaca
    11161 agaactaaag agttatgatg tttcgtacac tactgaacaa atggagagta tatatagtag
    11221 agatcattaa aaatgttcaa gatattttag gagtgattat ttttgaaaat atgttatttg
    11281 atattggcat acaatgatat tggcaactta aagagattgg tcaatcgatt gaacgaaaat
    11341 gcagttttta ttattcatat agataaaaaa actgatatag aaccatttat tgaagcattt
    11401 aaatcatatg ataatgttga atttttatgt aataaaaacc gtgttaaaat ttattggggt
    11461 ggtttttcca ttgtacaagc agaaatcaat ttagtaaaga gagcattaca aaatgagaaa
    11521 tatttaaaat atgtattatt atctggtgct gattatccaa tcaaagataa tgagtatatt
    11581 tataattatt ttaaaaagaa tagttcagtg gagtttattc gtggaataga tctagatcaa
    11641 attaaacata aagaatttta ttataaacat atagatgtat atcaaaaaca tgattatcct
    11701 agaattaata ggaataacac aactgcattt aaaattttta gagctataat taatcgttgt
    11761 ctgaggatga ttaaacttcc cccaaaaatt agacaccata aatttgattt gtatcatggt
    11821 tctcaatggt gggctttgtc gaaagaatgc ctaacagaat taatacaaat gtatgaacaa
    11881 aatcaacaag actacctgaa ttttaaaata ggtatgtttg ccccagatga aaaatttttt
    11941 catacacttt tttttaattc ctcgttcaaa aataaaaatg ttattggagg accagattta
    12001 ccacttgaat tgaaaaatat tgaagaaact actttacaaa cttcgaaatt agcaaatatt
    12061 catattattg atccttccat gaacaaatgg tttgatgaaa gggatataga tcaaataaaa
    12121 gaaagtgaaa aattatttgt aagaaaagtt aaatcggatt actctgataa actattaaat
    12181 agaattgata aagaaatttt gaaagttgga aatatagatg aatagaatag tacctgtttt
    12241 agagaagagt ttattaagtc gagatgcgtt tcgagtaata aatttaatca tttgtctgct
    12301 ttattttatg cctagtactt ttaatgtaac taatatcccg atgaaaatag cttttgcttg
    12361 gggtggaatt atagttttgt atgaggtttt tataaagaaa aattttttta aaatgcgttt
    12421 ttcttggcta ctgattggag caatgattag ttttatctta actataatca ttaacagaga
    12481 aaatttttta attccatcta tttataattt tggatatttg ctgattactt tgatagtaat
    12541 ttttcctgca gacttttcac aaagtgaaga ggaaaaaaag aaaaaaatgg tgtattttaa
    12601 taatgttttc
//
LOCUS       DADXTH010000080        12135 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.080, whole genome shotgun sequence.
ACCESSION   DADXTH010000080 DADXTH010000000
VERSION     DADXTH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.080"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1003
                     /locus_tag="IWI97_002269"
     CDS             <1..1003
                     /locus_tag="IWI97_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBC2463756.1"
                     /translation="IYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFG
                     YTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLF
                     ANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIES
                     NESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDA
                     KFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFF
                     RSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            1005..2075
                     /locus_tag="IWI97_002270"
     CDS             1005..2075
                     /locus_tag="IWI97_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463757.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            2062..3483
                     /locus_tag="IWI97_002271"
     CDS             2062..3483
                     /locus_tag="IWI97_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBC2463758.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            3496..4503
                     /locus_tag="IWI97_002272"
     CDS             3496..4503
                     /locus_tag="IWI97_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBC2463759.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            4515..5519
                     /gene="neuB"
                     /locus_tag="IWI97_002273"
     CDS             4515..5519
                     /gene="neuB"
                     /locus_tag="IWI97_002273"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBC2463760.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            5516..6664
                     /gene="neuC"
                     /locus_tag="IWI97_002274"
     CDS             5516..6664
                     /gene="neuC"
                     /locus_tag="IWI97_002274"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBC2463761.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            6637..7314
                     /locus_tag="IWI97_002275"
     CDS             6637..7314
                     /locus_tag="IWI97_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBC2463762.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            7304..8446
                     /locus_tag="IWI97_002276"
     CDS             7304..8446
                     /locus_tag="IWI97_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBC2463763.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            8459..9436
                     /locus_tag="IWI97_002277"
     CDS             8459..9436
                     /locus_tag="IWI97_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2463764.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            9436..10344
                     /locus_tag="IWI97_002278"
     CDS             9436..10344
                     /locus_tag="IWI97_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBC2463765.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            10345..11097
                     /locus_tag="IWI97_002279"
     CDS             10345..11097
                     /locus_tag="IWI97_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBC2463766.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            11102..12049
                     /locus_tag="IWI97_002280"
     CDS             11102..12049
                     /locus_tag="IWI97_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBC2463767.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         4354 a         1524 c         2207 g         4050 t
ORIGIN      
        1 tatctatcaa tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact
       61 tgcaagacaa ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg
      121 agctatgttt ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat
      181 aacaaaaacg attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac
      241 actttcttcg tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt
      301 gttgtttgcc aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa
      361 atggatggta agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc
      421 taaggatttt ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc
      481 aaatgaatca gataaaactc cacttccaga aaaagaagta atattacagc attctagtga
      541 taactccgaa atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag
      601 accaactttg ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt
      661 tttgaaaaaa gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa
      721 catgcataat ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat
      781 ctatgctttc tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt
      841 gaattatgcg tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat
      901 ggtggaggct catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta
      961 tttgggaagc atttcttact tgtatttaaa aaaggagaat taatatgaag acagctttca
     1021 ttgcttggac tcctttacat ttgattaata tattaaatgt ttatcaaaat ttttaccgag
     1081 aatcaaaagg agatttattt atttattctg aatttgaaaa cgctagaaga tatagtgagc
     1141 gtataaaaga attagggcta tttaatgatg ttgtttttgt gaatcaccgg aaaatcggtt
     1201 cttcctttaa acgtaaaatt aacttgttaa ctaaccataa tcaatttttc tcggatgatt
     1261 tatccgttta tgatgaaatt tttatacagg gggaaaatta tttcacaaaa atacttttct
     1321 ccaatgttta caaaaaaaat ccagatgtaa aactaaatta tatagaagat ggtttgggta
     1381 cttatttgaa gaagagtata ttttcaacaa gtaaaaacgt tcaaagagtt tttaaaatat
     1441 taaataaaaa tagcatcttc aaaaaaaaaa ttgacgatta ttttgtctat tgtccggaat
     1501 tgttacttta tgatgtaaaa aatataaaaa aaattcccaa aatagatgaa aacagtaaat
     1561 tgatgaaaaa catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta
     1621 tcttttttga tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg
     1681 ctaaagtaaa tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag
     1741 caaggacaaa tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct
     1801 tgctacttga aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt
     1861 tttcaccatc tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt
     1921 taaatgaata tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt
     1981 tctgtgaaca ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag
     2041 aattaagaaa gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt
     2101 aagtccagtt tctggtatac tatagggaat ttttttatta agggaatttc attcataact
     2161 attcctattt ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact
     2221 tcatttgtat caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat
     2281 gcaagttttg actataaaga tgacataaat gaatttatgt catcggtact ttctctagct
     2341 acactttcgt taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct
     2401 ttttttgata tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt
     2461 gtaattagtt atatccagtc ttattttacg attaacttta accattttaa atatttgatt
     2521 ttatctttta tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta
     2581 tattttgatg ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata
     2641 ggtttagtaa tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag
     2701 tggagatacg ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt
     2761 ttagcacaat tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata
     2821 tattcctaca ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac
     2881 gcctgggttc catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa
     2941 gttagtaact attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg
     3001 attgatttag caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt
     3061 ccgataacct taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt
     3121 tatgagaaaa aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata
     3181 ctattaaaca tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta
     3241 tttacgtatt ttttactttt tatttttcat tataatatcg ctaaaaaaat tacgaataaa
     3301 caattatttg acacaaaaaa aatatgtatc ataataattt ttgtatcaat tttttctttg
     3361 atacttcata ttttaatcaa tcaaatgttg attcgttacg ttttgacatc attattgata
     3421 attagtttag ttatatattt tttaaagatt aagaatcgaa ttatagatga ctataattca
     3481 taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg aactggttcc
     3541 attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat cagaatttat
     3601 agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca cactaatttg
     3661 agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg tcgtgatgtg
     3721 gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata taatcctatg
     3781 gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc gatcaaaaat
     3841 aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac taacaccatg
     3901 ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta taaaggaaaa
     3961 acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag tggatctgtt
     4021 attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac agatgttgat
     4081 atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc tgctgctatg
     4141 aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat tttaacttta
     4201 gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag aggtgaaata
     4261 aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac tacagacgaa
     4321 attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta tcatgctact
     4381 gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc agatgcaaca
     4441 gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt cgtgacttgg
     4501 taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt gtaaaccata
     4561 atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt ggagctaacg
     4621 cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct ccaatggcaa
     4681 aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta aaaaaattag
     4741 aattatctca atctgatttt attagaataa aagattattg tatacaaaat aaaattgatt
     4801 ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag
     4861 gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt
     4921 atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg
     4981 tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta
     5041 gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg
     5101 ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg
     5161 ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag
     5221 cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat
     5281 ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa
     5341 gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc
     5401 ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca
     5461 aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa
     5521 aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga
     5581 gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca
     5641 taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt
     5701 tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat
     5761 aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga
     5821 tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca
     5881 tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa
     5941 aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg
     6001 ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa
     6061 tagattacct agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa
     6121 atacgttttg caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat
     6181 tttagctttg atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa
     6241 tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat
     6301 tatttatatt acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt
     6361 tatgatcgga aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat
     6421 aaatttagga accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct
     6481 aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag
     6541 cttgaaaaat gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac
     6601 attgaatcaa atattgaatg atggggaaga tattaagtgg attcaaaaac aaatagctta
     6661 ttaaaatata ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt
     6721 attgaaacct taagattaga aaataagtta atatatggat tttttgatga taagcaaaat
     6781 aaaatgcaag atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc
     6841 aaaaaaaaaa tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt
     6901 ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata
     6961 gtggcaaaag aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac
     7021 ccagatgctg taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat
     7081 tgtagaatta ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat
     7141 gtaggtgtag gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga
     7201 tcttggtgta tgataggcgc agggagcact attgttaagg atattccctc acattcctta
     7261 gcttatggca atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt
     7321 attatcaagt cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga
     7381 taattggata gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata
     7441 tactggttct aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc
     7501 cttaagactg cttaatgttg gaaaaaacga tattgtcttt gctccttcat ttacttttat
     7561 agctacagta aatcctattt tatatcaagg ggctgtacca gtgttcattg actcagaacc
     7621 taatacatgg aatatgagcc ctgttgcact agagagcgca ttagaagaag ctgaaagaaa
     7681 caaccaatta cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg ctgattatga
     7741 acgaatttta gctattgcaa accaatttaa tgttccagtg atagaagatg cagcggaatc
     7801 gttaggttct acttataagg gaatttcgtc aggcttattt gggacaattg ggatctattc
     7861 atttaacggt aataagatca tcacaacctc aggtggagga gctctagtgt ctagtgataa
     7921 aaaatatgtt gagcattcaa gatttttagc aacacaagca agagatcaag caccgtatta
     7981 tcaacatagc gaaataggtt ataattaccg tctaagcaat atttctgccg gtatcggacg
     8041 tggacaaatg gaagtattga atgatagagt agctaaacga agagaaattt tcaatcgata
     8101 taaggaagct ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg atggcatggt
     8161 gccaaatcgt tggttgtcta ctttaacgat caatgattat aattctattg atcaagtagt
     8221 taacgtaata aataaactaa atgaaaaaaa cattgaaaca agagctttgt ggaaaccact
     8281 acatttgcaa ccagtatttc aaggggtaaa aatgtattac catgataata atcttagtgt
     8341 gtcagaaaag ttatttaata gcggcttatg cttaccttct ggaagtaata tgacagatag
     8401 tgaacaagaa tttgttattg atgagtttaa aaaaatattc aagtaggtgt gatattttat
     8461 gaacatagga attattggtt taggttccat ggggaaaaga agacttagac tcattcaaga
     8521 acactttaat gatattttac tatgtggtgt tgatactaat gaggcgagac gaaaggaagt
     8581 tgaaaaggaa tttggaatac caacttataa tagtctaaca gaggcaaaaa atttgtttga
     8641 aataaattca gtttttgttt gtacatcccc tataacccat gaatttatta taaaagaggc
     8701 tttaaattta gattgtaata tctttacgga aatcaattta ttaaatgaat attatgatga
     8761 aataatcgaa gtagctaaaa agaaagatag attactttat ctatcttcta catttttgaa
     8821 gagaaaagaa attcaatata ttaagtctaa agtgacgcat aataaaaaaa tttcttatca
     8881 ataccatgta ggacagtatt tacccgactg gcatccttgg gaagattatc gcaatttttt
     8941 tgtttcaaac aaaaaaacta atgggtgtag agaaatattc gctattgaat taccttggat
     9001 tatagataca tttgggaaag ttaaatcgtt tactgtaatg acccagaaat tatccaatct
     9061 tgatattgat tattcggaca gtttcactct tattattgag catgaaactg gtatagttgg
     9121 aactttaagt gtagatgttg tttcaagagt agctaaaaga aatttaacta taattggtga
     9181 aaagatttta atagaatggg aaggtaaccc ggattctcta ttttcatgga atgaagaaac
     9241 ttctagtatg acagatattc aattgtacga tgaggttatg catgataata attattcaca
     9301 tactattatt gaagatgcat atttagagga gattaaagaa tttttcacgc ttttggaaaa
     9361 gaaaatggaa ccccaatata cttttgaaaa agataagtat attattaact taattaacga
     9421 cattgaggta aattaatgat gaaagtaatt ttttttgggt tagggtcaat tggaaagaga
     9481 catctaaaaa atctaataag ctatggaaaa aagaattcta ttaattttga tataacagct
     9541 tatagtagca gtggaaaaaa agagtttgaa gaaataacgt atattcaaaa tagagacgat
     9601 ttggatacac attatgatat agcttttatt acaaatccga cttttaagca tctggaaaca
     9661 ctaaatgtaa ttgttgaaaa agttgattac atatttttag aaaaacctgc tttttctgag
     9721 agtattgatc ttactcaata taattatgat tacaaaaata tttatgttgc tgctccactg
     9781 aggtataaaa atatcatgga agttgttcgt aatagtattc aaagtaaaaa agtatattct
     9841 gtaagagcta tttgttctac ttatcttcct gattggagaa aaggtactga ttatagaaat
     9901 aattattctg cctttaaatc tatgggggga ggagtagaat tagactgtat acatgaattg
     9961 gattatatta taaacctatt tggtttccct aaatcagtaa aaaaacagtt tggtaaatat
    10021 tcgaatcttg aaattaacag taatgattta gcaacatata tattgagata cgatgataaa
    10081 attatagaaa ttcatttaga ttattttgga agagttccta caagaaaatt tcaattgttt
    10141 actttagatg aatgtatcaa tgttgactta ttaaataata aaataagttt tgaagaaaac
    10201 attgttaaag aaatgcctga atctgctaat gatatgtatg agaaggagtt gaaatatttc
    10261 tttgaaaacg tagtaacagg caaagaaaac tggaatgatt tatatcatgc aaacgaagta
    10321 ttgaaaatag ctgaggagaa ataaatggaa attttattta ctatttgtgg tagagctggg
    10381 tctaaggggt ttaaaaataa aaatttgaaa tatttggcag gaaagcccct agtccatttc
    10441 gctttagcaa tgatagatat ctatcaacaa aaacatcctg aacataattg cacgacttgt
    10501 ttgaatacgg acagtaatga gttagtagaa ttgacgacta agttaaatcc gtctgtgaaa
    10561 attgtagaac gtaaacctga attagctaca gatactgtag ggaaaattga agtgatacgt
    10621 gattcattct tacaactaga aagagaagat aatatatttg atttaattat agatttagat
    10681 ttgacttcac ctttacgtga agtttctaat cttgaagaat taatagatga acatctaaaa
    10741 aatttagata aagacgtcta cttttcagtt gtttctgcaa gacgtaatcc ctattttaat
    10801 atggtaaaaa gaattgaaaa tgaagttact atagttaata aatcaagata cacatctagg
    10861 caacagacac ctgaagtttt tgaaatgaat gcctcaatgt attcttttaa acctgaattt
    10921 ctaaaaaaaa gtaattatat ttttgatggg caatgtggga taataaaaat gagagattat
    10981 ctaattttag atattgatag tgaagaagat tttaaatggt tagaattttt gtttcctaaa
    11041 tttttagaag attcttcgga gcttcaagat atctataatt atttatatcg gaggtaaaag
    11101 gatgagctgt tatcttatca ccggtggggc tggcttcatt ggttctacct tagccaatca
    11161 tttaggagaa gaacatgtgg taatcgtcgt cgatgactta tcaatgggaa aagtagaaaa
    11221 tcttgatatg acgaaaaata tcacttttat tgagggagat gtcgctgaga aatccttgat
    11281 ggaagagatc atgaaagcta ataaatttga ttatatcttt catttagcag cagttgctag
    11341 tgtggcagat tcagttgccc gaccagtaga aacacatcgt gtgaattttg aaagtgtatt
    11401 aatgatttta gaattgatcc gcaagtatca acctgattta aaacggatcg tgtttagttc
    11461 ttcagcggct gtttatgggg atgaaccgac tttaccaaaa aaagaagagt ccgtgattcg
    11521 cccattgacg ccttatgcgg ttgataaatt cgctgcagaa caatatgtgc tagattattg
    11581 tcatttatat gatgtacccg gtagcgcagt tcgttttttc aatgtctatg gtccaaatca
    11641 aaatccgaat tctccataca gtggtgtgat ttctattctc gtagatcgct ataagaaaca
    11701 aatcgtagga gaagcaactt catttacatt atttggtgat ggaagccagt ctagagactt
    11761 tgtttatata gatgatgtaa tccaagcatt acttttagtt gcgaatgagg aagctgcact
    11821 ggggaaacaa ttcaatgttg gaacgggtaa agcaaccaca cttttggaat tgattcatgc
    11881 aatcaatgaa attctcggtg tagaattgac acttgaatac aaagaagagc gttcgggtga
    11941 tatccatgac tctctagcag atattactaa gatccagtca attggctatc aaccgaaata
    12001 tggtatttta agtggaatga ctaattattt gaaaactgaa atcaattaaa acatactaag
    12061 ataagattag agggtaaatt caggctcttt gtcaataagg actgatgagt tttgtaaaat
    12121 caaatctggg ataga
//
LOCUS       DADXTH010000081        12002 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.081, whole genome shotgun sequence.
ACCESSION   DADXTH010000081 DADXTH010000000
VERSION     DADXTH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.081"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(19..1827)
                     /gene="uidA"
                     /locus_tag="IWI97_002281"
     CDS             complement(19..1827)
                     /gene="uidA"
                     /locus_tag="IWI97_002281"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBC2463768.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(1850..3616)
                     /locus_tag="IWI97_002282"
     CDS             complement(1850..3616)
                     /locus_tag="IWI97_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBC2463769.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(3629..4423)
                     /locus_tag="IWI97_002283"
     CDS             complement(3629..4423)
                     /locus_tag="IWI97_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBC2463770.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(4433..5809)
                     /locus_tag="IWI97_002284"
     CDS             complement(4433..5809)
                     /locus_tag="IWI97_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2463771.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(5929..6576)
                     /locus_tag="IWI97_002285"
     CDS             complement(5929..6576)
                     /locus_tag="IWI97_002285"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBC2463772.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(6660..8087)
                     /gene="uxaC"
                     /locus_tag="IWI97_002286"
     CDS             complement(6660..8087)
                     /gene="uxaC"
                     /locus_tag="IWI97_002286"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBC2463773.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8161..9240)
                     /gene="uxuA"
                     /locus_tag="IWI97_002287"
     CDS             complement(8161..9240)
                     /gene="uxuA"
                     /locus_tag="IWI97_002287"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBC2463774.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9243..10874)
                     /locus_tag="IWI97_002288"
     CDS             complement(9243..10874)
                     /locus_tag="IWI97_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBC2463775.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11178..11849
                     /locus_tag="IWI97_002289"
     CDS             11178..11849
                     /locus_tag="IWI97_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2463776.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         3859 a         2392 c         1555 g         4196 t
ORIGIN      
        1 attcttaaaa aatccaaatt attttttata actgaaattg ggtattgatg accatcgttt
       61 ctgtagatag taagcaatca ttttaggttt tctatctcta gtaaagattc ctttcttatt
      121 tccttgcact cttaaaatcc cttgacttgt tgcaaaatct gcaaaattcc acacttgttc
      181 accaacaaaa ttttcacatg tatctattac ttcgtgattt gcttcgtaat aagccacttg
      241 atactcttct gtaaacattg ttggtactgt atcatgaaat ccagctactg tatctgctcc
      301 atattctgta aacattattg gcgtatcagg acatctttcc atccatccat ctagctcttt
      361 tttcaacaga tattttgcat gtgccagatc ccctcccgaa acataccaac cataataacg
      421 atttaaacat aaaacatcga caatatctcc taccttacaa acatctggcg ttgattttag
      481 ataagtaacg attgttacag gtcttttctg cggatcaagt tctttagtta attcaactaa
      541 aggttcaaaa tattctttag caccttcgga atccgaatct ggttcatttg caatagacca
      601 cattacaaca cttgggtggt tcttgtcgcg ttctatcaat tccttgatca ccgttttatg
      661 agaattttct gttcgtatct ctttccacgt attatgtttt tccacattat catctaataa
      721 agtggccatg aaatttaaat gtaaccctac tgcaggtact tcatcaataa ctacaatacc
      781 ttctcgatct gctaatcgca taatttcttc tgagtaagga taatgtgatg ttctaaaaga
      841 attagcacct atccatttca ttaaattaaa atctttaata tttacagcct cattcaaacc
      901 acgtccatta acatacgaat cctcatgctt accaaatcct ttaaaataaa aaggtttatt
      961 attaatcaaa aatttgccat tggtaacctg aacactgcga ataccaaatt cttctgtata
     1021 ggtatcgatc actttattat catcaattaa actaattttc atactatata aataagcgtc
     1081 tagaggttcc caaaaaattg gattcttaag ctgaatcatt cctttctttc cttcagcttc
     1141 agctacaagt tgagcctctt cgtcatatat cgctattaag atttttccgg tccattctgt
     1201 atcgatattg aattctacac ttgcatccaa atcagttatt cttgtattaa tagtaatatc
     1261 acttatatag tctttcggag ttgtatataa cttaacaggt ctatgaatac ctgcataatt
     1321 gaagaaatca aaattaacta agttttcctt ttttatatcc ccattttcct gtactgtttc
     1381 cttcataatc cccacaggta aagtactatc gtcaataata ttgtttacag caactgtaat
     1441 tcgattattc ccgtctttta caaaccgatt aatttcagac tcaaatggtg taaacccacc
     1501 tttgtgttga gtaataaact ttccattcaa aaaaactttt gcttcatgtg ttgcagatcc
     1561 aaaacgaaga actaatcttt cttctaataa tcttttagga agagtcactt ctttttcata
     1621 ccacacccat ccaacatgat ttctaatgtt ctctgtttca aataaatcat tataagaaga
     1681 gggaactgaa atcggataac tctctttttc atttattggt tctaaagcaa gttcttcctg
     1741 taagtcatca tttgtatcta atttaaatct ccagagaccg cttaagtcaa ttacttgcct
     1801 cgtctctgtc ataatgggat acaacatata aaccactcct atctaaatat taaataaatg
     1861 tatcctttaa accacaataa gcatctattg gacttactcc cttaaattca gaaagaccca
     1921 ttagtttgtc caccaatatt tctaaagttt cttgttttcc atcataagta ttaatataag
     1981 tctttacttg aggtacatca gccaaatgaa acggacactg tacagataca aaaattgttg
     2041 gaatttcatg aatgtaaaac ggtatatccg gagttccttt acttgctggc cagacaattc
     2101 gttgaactgt gcctggatta acattagcta tattaatgat caagtcatat tttttagtta
     2161 aatttttaat tggtctctta gcagcatata ttccagctat atttttaatt ttttcttctt
     2221 gcgataatcc tttcaattct tcttcaggtg aaatgtattt ttcaacttca aatccttttg
     2281 ctgacaaaat ctgtttcaat aaatcaatgg cttgaggtct atttccagca actacttcac
     2341 caaacccacc ttttacccca tgtacatcta ctagtaaaat ccttttatat ttttctggta
     2401 caataggcca aatatcttgt ttatctttaa ctaatgtaat tgcttggtca gccacctctt
     2461 taaaaattga tttattttca attaaaccaa ttttttccag agcttcttgc ttaggtaata
     2521 aaatttgttc ttttgctttt ttgtgtaatc ccaatttagc ttttaaccct aaaattcttt
     2581 ttaaggcatc taataaccga tcatcagtga taattccttt acgatagcct tccatcatat
     2641 aattaaagtc ttcatctgga tcattaaaaa atagaaatag atcacatcca gctgcaatag
     2701 aagtgggcaa taattctgat cgtttcattg ccccagtcat tcctaacata tgactagcat
     2761 cagtaactac taatccatta aatccgagtt ttcctctaag taagtcagtg attaattctt
     2821 ttgataaagt tgcaggaagt aaatcttcat ctttcagatc aggattaaaa tgttttgaat
     2881 aagctggcat atggatatga cctgccataa ttgaaggtaa tccagcttct attaagcctg
     2941 agtatacttt tccaaaagaa ttatcccatt cttccgtacc taaactatta atactaaaag
     3001 atagatgctg atctcgttcg tcaataccgt caccagggaa atgtttagct gcaggcaaaa
     3061 ttccactctc ctgaatacct ttcatataag ctaaactaag ttctaaaact ttatcagggt
     3121 tttgtccaaa agatcgacta gaaatgatag gatttctcca atttctatta atatctacaa
     3181 taggagcaaa actccagtta catccaatag ccgatgcttc aatcccagaa actctcccta
     3241 attcataagc atatttcgca tcatttgttg caccaatttt tacttctaca ccaacttcag
     3301 taccatctgt acaagctccg ttaccacctg cttccgtatt agcagcaatt aatagaggaa
     3361 ttttactttc tgtttgtaag atatagtttt gatcatatac ctcatcagca gttcctggat
     3421 tatatcgtac agctccaata tggtactgat tgaccatatt actcaagtaa tttttatcac
     3481 gactagatcc catattaaca aacaattgac ctattttttc ttccaaagtc atagaatcaa
     3541 tcgtctcttc aatccaagaa atagcatctt catctaaatt atatggtttt tttcgtaaat
     3601 ctacttttat agtcattttc tttctcctct acttattagt aagatttttc caaaaagtat
     3661 ctatatattt ttgttcaata tctacataat ctcccgataa acatttaaat aaaacatcag
     3721 ttcttaagtt ttcccatgat tctttttctt tattgaccaa aataggataa aagaaaactt
     3781 tatttttatt tgcagcttct agatcaccag gagaatcacc aatcattaat atcttatcca
     3841 aatcacaacc attttcaatt acacttcgaa taatatcttc tttttttcct ttatcttgac
     3901 aacacagttc atcaactaat gataataatt catgtctttc ccattcttct tgaacagctt
     3961 ccttattagc tgagcttaca ataattatct tcccatattt tttcagagct tttatagtat
     4021 ctctagcact taaaaagggt ttatctaatc cttttgcttg actaatctgt tgattgactt
     4081 cttccgacca ttttaaagct tttaataaat cctctgactg agaatattct atttccctct
     4141 tcaaagatgg attagacaat tcttttgtta gagataccca gtgctttaat cgattaatcg
     4201 atgttacatc atttttttct aatgttttaa ctaaacctaa aaatctgttt attccacgag
     4261 tctctgaata taaattaata ttattccaat cctgtaggaa tttttctcta tgatgaacat
     4321 caaaaatctc tgcagcaatt ggtccgaaaa agagattgtg tttataagtc atcgtatcca
     4381 taacacagcc atctgaatca atacaaaata tccaattatt catatttctc tcctatttat
     4441 gtttttgttt taaaagctcg caattttcta agactttatc tttagataat ggataaagga
     4501 aaaataaaat taatcctaca atcagaaaac aaattgatgg aattaatgtt gaagcgttat
     4561 ataacttttc taaaacatct cccgtttgta catttgctaa agagtcatag cctataatag
     4621 aaagagtcca acctccgacg cctccagcta gagcttgacc tatttttcta gcaaaagaat
     4681 ataccgcata tacggtccca tcttctcttt gatcagactt tacttcttga tcatcaataa
     4741 catcagtaat atttgcccaa ataacagtat taaacacacc caaacccata tagccaaccg
     4801 aactaagaat taagaataca tacatattac tagcttttat caaaaataag atcaaatata
     4861 ctattgctga aaagaacatt cctactgatg ccagttcttt ttttccatat tttttactta
     4921 aaaacaaagt taaaggcgaa acaactatta aaaccaaaat aggattgata aaattcatta
     4981 aaactattcc ctgtgcatca ttatagtaat caggaaatac ataattattc atactcataa
     5041 ttaataattg agcaaggagt aagaaaatag ccgcaccaat tatagaaagt aatgccctat
     5101 ttttaactaa cccttttaaa gattttaaca atgaaacttt gtattgagat tggtcaattc
     5161 ttactcgttc agtagttagt ttataacata ttaaataaca aataagtgca catatcgaaa
     5221 atattcccgc aacaattgta aaagttgatc caccacgaac tatttgagcg cctgaatcat
     5281 ctcttgtata aataaataat ggtgttaata caccaatgac caaacttgct aaagtagctc
     5341 caacatttcg gaatgtcgat agcattgttc tttcttctgt aatagaagta atagctgatg
     5401 ccattgatcc ataaggaata tttatagatg tgtaaaaaat actcccccat aatatatacg
     5461 taacatacat gtatactacc ttaacgctca tagaagctga aatcatagaa gtttgataca
     5521 ttaaaaaact tgctaaagcg actggaccac tcattcttaa aatccacggt ttaaacttac
     5581 cctgcttagt tgctttcgat ctatcaacaa tacgccccat agttacatca gtaactgcat
     5641 cgataaatct tgctactaaa aataatgttc caacggcagc tccgcttatc cccaatactt
     5701 ttgtataaaa gatcattaaa aaagaactag caaaaatgaa agtaaaatcg tttccaaaat
     5761 ctccaaacat atatcctatt ttgtctctta taccaaattt tctttccata caattctctc
     5821 ccttctgttg ttaagcgttt tcattctgat gaaagtacgt actatactct gaagcgtaaa
     5881 gagtcagtat ttatttttta catttcagag tattatttgt tttttctcct aataattctt
     5941 tatctcataa aacttatcta tatatttact agctactcga cttacttctt caaaatcacc
     6001 tttttcaaca gaagccaata aattcccacc tacaccaact gcaacaactc ctacttcaaa
     6061 ccaatcttct aagttctcta tacttactcc gccagtaggc attatattta cgtgtggtaa
     6121 tggagcttga taggctttta caatggatgg accaaaagca gaacctggga atagtttaat
     6181 gatatctacg ccagcagtaa gtgcctcctt tatctcagta attgtcatac aacctggtaa
     6241 ataaggaatt tgatataaat tacaaatctc agctgtttgt cgatcaaaag atggactaac
     6301 gatatattct gccccagcca taattgcgat acgagcagta aacgcatcta aaaccgtacc
     6361 ggctccgatt attacatctt tttgatcagc atatttttta gcaatttctt tgattatttc
     6421 ttcagcatta ggaactgtaa aagttagttc aatcccttta attccacccg caataattga
     6481 atttattgct ttaatggcct catccttatt tttcccacgt acaaccgcaa tgactccagc
     6541 cgcttctaac ttacttaaaa tttctacttt tttcatatct atctccttcc tttatggata
     6601 catactttgt ctagctgtac taacttacaa agaataaatt taagctttta tttcaagtat
     6661 tattttctac ttccgatttc aaaaaaatta aaataattat acgcattacg ataacaaata
     6721 ttctccacta tctgactaag atacatttca tcatctggaa ttcggcctgc tttaacccac
     6781 tcgcttagta attgacataa tactcgacga aaatactcat ggcgaggata gcttagaaaa
     6841 cttcgagaat cagtcaacat acctgtaaaa ttcgacaaca aactttgttg tgccatcgta
     6901 gtcaactgtt gacgcattcc ttcagctgta tcattgaacc accaagcaca tcccaattgg
     6961 attttttgaa tcataccccc ttggaagctt tgcattgctg ttgctagtgg aagccaatca
     7021 gttggattta atgaatataa aattgttttt ggtaagcacc cactcttttc agcggtatcg
     7081 aacaatgcta caacattttg tgtcaaacta gcttgatccc ccatcgaatc accaccgaca
     7141 tctactccaa tttgttcctt taatatactt gaattatttc ttaaagcatt taaatgcatt
     7201 tgcatcgtcc aaccatatct tgtatactca gacatcaaat gtaattgaat acctgtaaca
     7261 aattgtgcaa tttctgataa atggaaatcc gtttttcctg ctaaagcttt tattaacaga
     7321 ttgtcgagta ttttttcatc tgctatttca taaaaataag tattcaaacc gtgatctgct
     7381 aatcgacagc caatttgatg aaaatgatcc actcgttggc tcaatgcttg cttcaaccca
     7441 ttaaagtctt tgattggtat agccgctgtt tgactaagtt ttaaaatata gttcgaaaag
     7501 cctgatgatt tgatactcca gaccttatct ggacggaaag taggtaatac cttgaaagaa
     7561 ttatcctgtt tagaaagctc agtatgatac cacaagtcat ctgtaggatc atccgttgta
     7621 cagatggctt tcacatccat catctccaac aattctttcg ggcgatagga atccgtttga
     7681 attttttcgt tagcacgtcg ccaaatttct ggtgcattct tctcagaaat tgtcaaatca
     7741 atatcaaagt aacgttgtaa ttccaaatga gaccattcaa aaataggatt acctggcgcc
     7801 ttctccactg tttttacaaa agctaaaaat ttttcataat catcacctgg accactgatc
     7861 aactcttctt ctataccatt agctcgcatc aatcgccatt tataatgatc tccagctcct
     7921 ttatcattta accatacctg agtcaaattc ttgtattgct tattttctag caaatatgct
     7981 gggtctaaat gacaatgata gtcgataatt ggttggtgtt ttgcaaattt atgaaaaagt
     8041 cttttagcta tttcattctc taataaaaag ttcttatcta aaaacatatt taaacactcc
     8101 tttccttccg ttagttttta gaagagaaaa tttttcctct aaataaactc ccttatataa
     8161 ttatttactc gccccctttt caagcgcttc ccaaatacca tttaaataaa ctgcacctag
     8221 agctcggtca tataatccat aaccaggtct accggtttct ccccaaatca tccgaccatg
     8281 atctggacga atatagccat taaatccatt atcgtacatt gtttttaata tcttatacat
     8341 atctagagaa ccatattctg atttatgcgg cgcttcgtaa aaatcgagtc cattgtcact
     8401 taaatatttg atattacgaa catgtgcaaa aggaatccga tcccgttgac aaaattcaga
     8461 tagaatttca tagacatcat tatctggtcg ttctgcaata cttccagtac ataatgtaat
     8521 accattagaa ggagaatcca ctacattaca caaccaatct aaatcgtcac gatttttaac
     8581 aatacgaggt aatccgaaaa tggaatatgg tggatcatct gggtgaactg ccattttaat
     8641 cccaacttct tcacatgtcg ggataattgc tttgagaaaa tatgcaaagt tctctcgtaa
     8701 tttctcttca tcaacttctt tataggcttc taataattgc ttaatttgtg ataaccgatg
     8761 aggttcccaa cccggcaatt caaaattatt agtagaattt tctacttctc taataatttc
     8821 ctccggatca gtagggatat tttttgaaat ataggctaac gtagaagacc catcatctaa
     8881 acgataatcc aaatccgatt ttacccaatc aaatactggc ataaaattat aacagataac
     8941 ctcaatccca aattctgcca aattgcgaat cgtctgttta taattctcaa tgtattgatc
     9001 tcgtgttggt aaaccaattt tgatatcatc atgaacatta acactctcaa taactttcaa
     9061 ggtaagtcca ttggcttcga cttcatcttt taaagattta attcgttctc tcggccaaac
     9121 ctctccggct ggtatatcca ttaatgttcc aacaattcct ttcattgctg ggatctgacg
     9181 aatttcttgt agtgagattt tttcttcatt tgccccatac catctaaaag tcatttccat
     9241 agttactttc ctccaacttc tgttatatat ttattgattg ttttacgaac tgcttgagga
     9301 cctactagca tttcgcaaaa gattttttca gttttctttc ctaccccaac ttgatataaa
     9361 tctaaaccaa agatttcttt attttctaaa atttcctgta ccgcacaatg aatctttgaa
     9421 ggatcttgtt cacctaattt caatactgat aatttatcct gcaactctac caataacgga
     9481 tcaggacttg gcacaaaatt ttccccttga tcatcgactg ccagtaaata acgtaaccaa
     9541 gcagcaatag cgataggaat aaatgttaga ttttctgcat ttccgtcttt ttcgacatac
     9601 tttttaattg tttcaccata gcgaatcgct actttttgag aagtatctgt tgcaattcgt
     9661 tgtggtgtat ctggaatata gggattaggc aaacgtttat taattacttc atcaataaat
     9721 tgttttggat caataatgcc tggattttca actactggta aaccttctaa ataacctatt
     9781 cctttgatta aaccaaccaa atcatcatcc tgcatctctg ctgaaattga ttgatatcct
     9841 agcagacaac ctgttactgc caaagcagta tgaagtggat tcaagcttgt cgtaaccttc
     9901 atgatgtctg ctttatcaac cgtctctcta tctgtcataa tcacaccagc cttttccaat
     9961 ttaggccggc catttggaaa actatcttca ataactaaat aatgaactac ctcagtatta
    10021 gtgaaagcag caatattggt atgttttttt gtgtgaatga tatctaaatc ctcgagcccc
    10081 atcatcaata gatcttcctt tacattctct gaaggatttg gcgtaattcg atcaatcata
    10141 ctccaaggaa aagtcacttt ttctttatcc tttaaataag caataaattt ttcagaaaca
    10201 tatccttctt tttgccaagc ttctgcaatc gtaaaaatac tatcttgaaa acgttgacca
    10261 ttctgtgaga aattatcagt agaaaccatc gcaatcggca actctccagc attaaatctg
    10321 ctatataaca aggatgttac aatactcatc gtatgttgag gattgctagg tccagatttg
    10381 atatcagctt tgactaccga aaagaattgc cctttagaat cttttaaact gtaacctttt
    10441 tcagtaatag taaacgttgt aaactgtaat ttaggatctt caaaatattt aaccactctt
    10501 tgatagtccg ctatattctt cggattgcaa tataaactat cagccgttac tgccaaaagt
    10561 ctttttttga gttcaccatc tccactcata acaatttcca aaatgtcatt gttatagtca
    10621 tgataagctt tttccactac ttcttcatca aatgtttcac acacaactac tcctgtttct
    10681 aattcatttt gatctgctaa gcattgagca atttctgcat gaaatcctcg ataaagattt
    10741 cctccaccaa aatgaagcca tactggatgt tgctggcctt cttgcttaac ttctttgaca
    10801 tcaaaacacg gcagtagaat ctgcatttca ttgaattctt cgatagaatt ttgccaattt
    10861 ttaatcgaaa ccataattaa cctcctcaaa ctgatatgtt agttttgata cattttatat
    10921 tttaaaaccc attgtaaccg ttgtcaatat aaaaagggaa taaacttgct tttttctacc
    10981 atatcagaat gattgtattt tttattcaga aaaataaaat actccttctt tttggactga
    11041 ttttatcgaa acttattttt tctttttttc atgtattctt ttatttttat aactagtgta
    11101 ttacttcaaa atttgatatt atatggcatg aacagaatta aaatatcagt taaaataaaa
    11161 gttaaggagc attgtgtatg acaattaaaa ctagtctcca aaatcaagct taccaatcta
    11221 ttcgccgaca aattatctat gctgacttag aacctggaaa aaaagtatca gaaaaaaaat
    11281 tagaagaaac actttctatt ggtcgtacac caatacgtga agcactgatt caactccgac
    11341 aacaaggtct tgttgataca attcctcaaa gcggaaatta tatctcatta attgatttag
    11401 attcggccaa aaatgctcga tttgtcagag aacatttgga acgacaaatt atgttagagt
    11461 gttgtgccaa aatgaataac caaaaaaaac ggattttaga aactataatt tcagaacaag
    11521 aaaaagctgt tagtcaaaaa aatgctcgta gtttttttca taatgataat ttattccata
    11581 aagcttgttt tgaaattgca gagcgagaag aagtatggca atggttagaa aataacaata
    11641 cacacttaga acgatttcga tggctacgag taaaaacgga aggattaaag tgggaaaaaa
    11701 ttatggaaca acattatcaa ctatttgagg cattaacaaa acaagatcca gaagaagcta
    11761 actttctaac atcgttgcat cttcatttaa tgcttaatga gcaaaatatt gttttagcta
    11821 ctcatccaaa atatttcaaa aatatataac ttttaactct ttttaaatta aatggttgtt
    11881 ctgatcagaa aaatatcgct tgttttctta ttatgtaata tatttcataa actattctgc
    11941 ataacaacta ttgtttgtac tttttacatt ggataactaa ttatgactgc tgataacttt
    12001 tg
//
LOCUS       DADXTH010000082        11521 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.082, whole genome shotgun sequence.
ACCESSION   DADXTH010000082 DADXTH010000000
VERSION     DADXTH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.082"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IWI97_002290"
     CDS             complement(<1..281)
                     /locus_tag="IWI97_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2463777.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLY"
     gene            complement(429..987)
                     /locus_tag="IWI97_002291"
                     /pseudo
     CDS             complement(429..987)
                     /locus_tag="IWI97_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302360.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1156..3267
                     /locus_tag="IWI97_002292"
     CDS             1156..3267
                     /locus_tag="IWI97_002292"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF019826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2463778.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTL
                     LFLSFNTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYR
                     IRYELPFEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCS
                     ELLNSSIIETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVED
                     VDLIFSNQPLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRY
                     SAVQNEESLQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTW
                     QNAMELSCQPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGC
                     SLLISVDPIIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTED
                     EVRKKIKNFLRRKQYVNNEFS"
     gene            3245..3895
                     /locus_tag="IWI97_002293"
     CDS             3245..3895
                     /locus_tag="IWI97_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBC2463779.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            3882..4334
                     /locus_tag="IWI97_002294"
     CDS             3882..4334
                     /locus_tag="IWI97_002294"
                     /inference="COORDINATES: protein motif:HMM:NF012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2463780.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            4351..4656
                     /locus_tag="IWI97_002295"
     CDS             4351..4656
                     /locus_tag="IWI97_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HBC2463781.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            4668..5945
                     /locus_tag="IWI97_002296"
     CDS             4668..5945
                     /locus_tag="IWI97_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIABC"
                     /protein_id="HBC2463782.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            5964..7112
                     /gene="dgoD"
                     /locus_tag="IWI97_002297"
     CDS             5964..7112
                     /gene="dgoD"
                     /locus_tag="IWI97_002297"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBC2463783.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            7124..8026
                     /locus_tag="IWI97_002298"
     CDS             7124..8026
                     /locus_tag="IWI97_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HBC2463784.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
     gene            complement(8695..9081)
                     /locus_tag="IWI97_002299"
                     /pseudo
     CDS             complement(8695..9081)
                     /locus_tag="IWI97_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9141..9821)
                     /locus_tag="IWI97_002300"
     CDS             complement(9141..9821)
                     /locus_tag="IWI97_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HBC2463785.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
     gene            9904..10053
                     /locus_tag="IWI97_002301"
     CDS             9904..10053
                     /locus_tag="IWI97_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HBC2463786.1"
                     /translation="MKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPL
                     ENELR"
     gene            complement(10802..11415)
                     /locus_tag="IWI97_002302"
                     /pseudo
     CDS             complement(10802..11415)
                     /locus_tag="IWI97_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         3699 a         1816 c         2285 g         3721 t
ORIGIN      
        1 ttgtacaggt gtagataagt tggtacttct tgatacggaa gcaactgaat ccaactatca
       61 tgataagcat gacggatgac agaatcaaac ccacaacatt cgcaacgatg cgcttctcca
      121 gttaataagc cggtaaatac attggccata cggccatgaa cgcgctcttt actaatggca
      181 tcttttgaaa aggtaatatg ggaatctttg atgttcaaaa tttgtttgat ggaatgactt
      241 gtcttttcat ccgtacattt gatattctta gttatggaca tttggttatc ctcctatgat
      301 tgtttttgcc gatttaatta taggacaacc agatgtcttt ttgttataaa aaaatatttg
      361 gcgtcactac tcagtttgag taacaaaacc aaatattata gaaccggctt tttcagtttt
      421 ttcccttcac aaacaacatc gatgattttc tcggcatggg tacgggtatc attcattaat
      481 tctgtatcgg ttggatattt gatgttttgt ggagcacaag tggcatccaa aatcaatgtt
      541 ccggaattga aatcgtcata agaatcgttg tccttatcgt cttttttctt gtcgaagacg
      601 agtatttttt cattgatttc tatcaacgtt gtttcgtcca agcgtttccg gaaatagacc
      661 attgtagagc atcgaaaggc ttggcatctt gatagcccgg caaaccgatg aagaactata
      721 aatacgggtt ttcttgaatt tgtaagaccg tttcttcgtc gctatagcta tattcctttt
      781 gaatcagtag agctccgaag gtcatgcgaa agggttttgc gacgtttcct tttttttacg
      841 aaaattttta gcgtagtctt tttcaagttc agcccatgga atgagctctg ctttttgaat
      901 ccaacggtta gacggattca ttttgagacc tatcggttga ttgaaatctt caaaagccaa
      961 ttgaacggtt gtttgtaatt tatacatgaa aagcctccaa aattgagtgg tactccacac
     1021 cattctgatg gtgcataaga taaaaaatta aagaagtgcc tgtcatttgt ctgatttaag
     1081 ccaaggtttg acaaattttg gatattccaa ttaatactgt aagcggttat atttaactca
     1141 gggaggtaga tgaatgtgaa tacaaaagat ttaaaactgt tagaaaccat tgtacgatca
     1201 catgtgacaa actctcaaca attgaaatca ttagtacatt tttcgactag gcagataact
     1261 tactctataa aaaaaattaa tgatgaatta tcccttcatg gattgagtct gataaagcga
     1321 agtaactcag gggagtggtt atatgaagcc aatgatcttc aaaaactttt gatatacatg
     1381 aatcaagcaa tggtttttaa agaaggtgcg attcctgaat atataaacga tgatttggct
     1441 tatagtgaga taagaagata ttcggaactg ttatttattc tagccaaccc caaaaaagtg
     1501 acaacccaag aaataatggc gtttattggt atcagtaaaa atacggctag aaacgacttt
     1561 tctaaaataa aaaaagagtt gccaccagac tacactttga tatttgatcc ccaaaatggc
     1621 tatatgattg aatcaagcat ggcaaagctt catacattgc tcttagaagt tttcaattat
     1681 ttatttgaaa ggcatgcaac agttaaccgt tttgagtcac tcttttctga agaaagggaa
     1741 tttgttggtt tttttattca cacctacgaa caaactttta aattgaaatt ttctgataat
     1801 tcactaacca aactttatta tttgataggt ttatcacttt ctagttttaa aagaaggacg
     1861 aaaggacaga ttgattttag ttatgattta caggaattag atgagtttca aatgattcga
     1921 agctgcattg atcaacatta tcaacatgct gagatagcta tagattatga atggttgacg
     1981 cttctgtttt tatcattcaa tacaatttca aatgagtttg aaattttaga ttcaagaatc
     2041 tatcaagtaa ttatggagat gattacctta tttcaaagta aatcagctat tcggattagt
     2101 aataaagaag agttgaccaa tcgattgatg aatcatttac gacctgcaat ctatcgaatt
     2161 cgctacgaat taccatttga aatagatgat ttagctgaaa ttgttaagga aaaccatgaa
     2221 gttagtgcgg tacttaattt tgttaaagaa tccattgtac ctttggaagc atttttgcgt
     2281 aagacgattc ctgaaactga attatatttg atttctgttt atttttgttc ggagctatta
     2341 aattcttcta ttatcgaaac acctgttaga aaaaaggcaa tcgtcgtatg tagcaatgga
     2401 ttgattatgg ccaatgtaat ggcgaaaaca ttgaaagaaa tttttccaga tattcagttt
     2461 gttgctagtt cttcggtcag agagatggat aagtttgtcg aagatgttga tttgattttt
     2521 agcaatcaac cattgaaaag tgaaattcca ttatatatta tacaaccgat tatgaacgaa
     2581 gaagaaaaaa accgcttgcg gaaccgtgta ctcattgatt tttctacgaa taatatcagc
     2641 aaagagacta ggaaccttac tgaaattatt aagcgttact ctgctgttca aaatgaagaa
     2701 agtctacaaa ctgatattga gagattttta attaattatg caaattcact aatatatcta
     2761 gatcgtcaaa gtaaacaggc acaaaagata tcttttgtgg aattgcttgt ttctgaaggg
     2821 attattcttg tatctacgaa acaacttact tggcagaacg cgatggaatt gtcttgtcag
     2881 cctttattaa aggagcaggt aattaatcag aactatcaga ctattttaat gagtcaaatg
     2941 gcggatgaag aggcttatta ttttcttaat aatcgtattg ctattccaca ttcatcgcca
     3001 gaaaatgggg taattaaggg cggttgctcg ttacttatat cagttgatcc aattattttt
     3061 cctggaaata ggcctatcca ttttatttcc ccttttgcaa ttggaaagaa ggaaaattat
     3121 ttacttgcga ttaaccaatt actacaactt tctaaggacg aggaatatca gcaagaaata
     3181 cttctttgca cgacggaaga tgaagtgaga aaaaaaataa aaaatttttt aaggaggaag
     3241 caatatgtca ataacgaatt ttcctgaatt aaccatcatt ttaagaggat atagttttga
     3301 agaggcgatg tttgtcatgg aagaattggc tgcgatgaac acttcggtag gaattgaagt
     3361 aacaaccaat aatgatgact ttgcacgtat catcacagaa ggcaacaaaa aatttgggca
     3421 acaattattg attggtgcag ggacagtttt aacattttca caagcagatc aagcaattgc
     3481 tggtggtgcg aaatttttat taggaccaaa aaaattttca acaccaattt tttcgttggc
     3541 taaagaacgt ggggtggtaa ctgtgccatc agcttttacg ccatcggaaa ttcaagttat
     3601 gtttgatgaa ggtgccgata ttgtaaaggt ttttccagca gtcactgtag ggccaaccta
     3661 ctttaaacaa ctggccggtc cttttggtat acggccttta atggcagttg gaggagtgac
     3721 gattgataat attgcggctt ttaaaaaagc aggagttgtg tactttgggg taggatcaag
     3781 catgtttcaa aaagctgata tacaaaaccg caatgggcaa gctattcgaa aatcaattca
     3841 atcatattta gataaactag ctgaagcagg aagtgaagaa gatgagtctc attaaattag
     3901 aagaaaaaaa tattttgtta aatcaaagtt tttcaaacca agaagaatac tttcaaaaga
     3961 ttacaaagaa actgatgacg gaaaacatgg taaaggaggg atatctcgag tcaatcacta
     4021 agagagaaac aacttatcca acggggctat atattgggga acgctcaatc gcaattccac
     4081 atacagatta tcagttatca aatgttaccg ctattttagt atccgttctt aaagatagtt
     4141 tgcattttca gcgaatggat gatcccgaag aaatggtttc agtgaatgtt attattcacc
     4201 ttttattcga tagtcccgat aagcaaccgc aattattgaa ggagttgatg agtaaagtta
     4261 ccaatcagga tttcttacaa gcaattatgg aagcaaagag taaaacagaa gttttaactg
     4321 catttaatca ataaattgga gggaaataca atgagtaaaa aaacaattat cgtcgcatgt
     4381 gcatcatcaa tgattacatc tacaatggca gctaataaaa ttcggaaaat agcaaaaaca
     4441 aatggattac cagaacctcg aattttgcaa ctaaaatttt cagaagttgc aggtgcttta
     4501 gaatcaaatc aagttgactt tatcgttcct acagggaaat tacaagaaaa tgtaaccaaa
     4561 ggaattaaag ttatacctgg aacaccattt gttactggag tgaatgaaga aaaatctgaa
     4621 tctttggtgt tagaagcatt gaaagagctt gactaggagg gtgaaaaatg aatattgtca
     4681 ttgatgcgtt aaactggatc tcaagtttgg gtccgatgat tatgatgcct atcattatct
     4741 tgattttagg attatttttt agaattaagc taaatacatt actaaaatca gcgttgactg
     4801 tggggattgg ttttgcgggt gtaaatatta ttattaattg gtttatttca caggtaggtc
     4861 catctgttca gttaatggtc aaaaattggg gtattcaaac aagtattatg gatgtcggct
     4921 ggccagctcg tgcagcagcc acttggtctt ttcctctagc agcagttatg gtatttatta
     4981 ttttgggtat taatgtgttg atgctgattt ttaaaaagac caatacagta atggtcgatt
     5041 tctggagtta taatcactat atttttgtag ctgctttggt ttattatgca acggataaaa
     5101 atattatatt ggcggtaatc ttcggggcga ttgatgcaat tattagtttt aaactcgctg
     5161 attggacaac gccgttagta gaggattact ttgaactaga aggggttaat ttcccgactg
     5221 cgaactcagt tggttgggca cccttggctt ggctacttaa caaattgtgg gaccgcattc
     5281 ctggattaaa taaggtagat gctcgtcctg aaaaaattca agagcggttt ggttttgttg
     5341 gtgagcctat gtttatggga tttgtcatcg gcttggttat tgggttatta gcaaaagaac
     5401 ctgttgataa ggtattgata gtgggaatga gtacttctgc aacaatggtt ctaacaccta
     5461 aaatgatgca aattttaatg gaaggattat taccctttgc taattctgtt aagaaattct
     5521 tgaatgtaaa atttccagga aacaatttta caatgggtgt ggatgctgcg ttgacagttg
     5581 ctaattcaag tgccattgct acgggtatta tcatggttcc gattacctta gtgttggctg
     5641 ctattttacc aggtaacaaa ctcttaccta tttcagatat tgcctaccaa gcaatgtggc
     5701 tatctgcatg gccggtcgcc ttttcaaaag gaaatattat tcgcggaata ctttccacca
     5761 cgattataac agcttgtgtg cttttaattg ctactagttt agccggcata catacacaat
     5821 tagctttggc tgggggattc caattaccag caggaatgac acaaatatct accgaagatg
     5881 caggtacaca cattattggt ttcattttaa tgaagattgc tgagtttatt tcaaatattt
     5941 tctaatattc aggagggcat acagtgaaaa tttcaaaaat tacagtctat aaagtaaaac
     6001 caagatggat ttttgtcaaa atatctacag atgaaggtat tgaaggctgg ggtgaaatga
     6061 tttctggaac taagactgaa acagttgtgg caggagctta tgagatagga aatcgattct
     6121 tagttggtca aaacccattt gcaattgaac gtctgtttca ggaaatgcat cgttcatttt
     6181 tccgaggtgg tcctattaat ggaacgattg tctctggact tgaaatggct ttatgggata
     6241 ttaaagggaa agccttaaat gttccagttt atgaattact aggtggagca gcccgtgata
     6301 aaatcaaagt atattcttgg attggtggag atcgtccttc tgaagtagta gagcaggcac
     6361 aagatcgagt agatcgtggg tttaccgccg tgaaaatgaa cgcgacagaa gaattacact
     6421 acattgattc ttatgaaaaa gtagctgaag tagtggaacg tgttgcttca attagagaac
     6481 gttttggaga tacattgagc attggtgtcg attttcatgg tcgtgttcac aaaccaatgg
     6541 ctaaggtttt agcaaaagca ctggaaccct atcatccaat gttcttagaa gaagtggttt
     6601 tgccggagaa tgaagaacat ttcagtgaag tagccaatgc agttgctgta ccactagcaa
     6661 cgggtgaacg tttgtataca agatggcaat ttaaatcaat tcttcaacag ggagcgattg
     6721 atatcattca accagatgta gctttatgtg gagggatttt agaaacaaga aaaattgctg
     6781 caatggctga agcttatgat atagcagttg ccccccatgc accatatgga ccgattgcgt
     6841 tagcagctac attacaagtt gattcttgta cacctaatgt gtttattcaa gaacaaagct
     6901 taggtattca ttacaacaaa gggtttgatt tgttagattt tgtaaagaat aaagagatat
     6961 tccaatacaa agatggcttc gtagatcttc cgacaaaacc aggtttaggg ttagaaatgg
     7021 acgaagatcg agtgaaagaa attgcacaag aaggtttagt ttggaccaat cctaagtgga
     7081 aaaattatga tggtacaatc gctgaatggt aggagatgag tgcatgatca atacggttac
     7141 aggcccaatc gaagctgagt ctttgggtac aacatttatt catgaacatt tatatgtcat
     7201 tcctaatgag ttaagaaagt atgaagatta taccttggat gatatttatc atagtattgc
     7261 ggaagttatg gcttttaaac aagctggtgg aaaaactatc gttgatttga caccaattaa
     7321 ttatggacga aatccgttag ctttgaaaat gatatctgaa gctaccggag tgaatgtagc
     7381 atttgttacg ggattccata aggaagagtt tcagccaaaa tggttgacaa cgctcaatga
     7441 tgatgagatc tttgagttgc tttattccga aataactcac ggagtcactt cacaaaacct
     7501 tttgccagga gcaatgaaag taggtacgag taaaaatgag gttacattgg ctgaacaacg
     7561 aatactagcc atagccggtc aagtacaaaa aaaaacaggg attcccatta ttacgcattg
     7621 tgatgggggg acgatgggac tggagcaaat taattgttta acaaaaaacg gtgcgaaagc
     7681 aagtaatatt tgtttgagtc atgtagattt aactaaagat tttcactatt tagctcaaat
     7741 ctgtgaaacg ggagcaagta tttgtatgga tcatattggc cgagagttgg cgaatcatga
     7801 tgcagaccgc gtccaactaa ttaaacgact ggttgatgcg ggttatgaaa atcagatttg
     7861 tctttcaggt gatatgggac gaaaaaaata tttgccaact tatggaggaa cgcctgggct
     7921 tacctatatt ttaacaagtt tgaaagaaga actattaaaa gtaatgagtg aaccaacttt
     7981 taataaaatg attgagatga atccaattaa tatactgagt aagtgaagtc aattgaatca
     8041 ttcagaagaa ctaatgaaat tagttgcttt cgcagggtta attcttctct ctgggtttgg
     8101 aaaagagggc ttagcttcgg attccctagt taaactaggg gttttatcag gttattgtac
     8161 tgattataag tacctgaatt attcatgaaa tactttttcc gtcgttttat aggcagttgg
     8221 gtcaataaaa agatcagaaa aacgaacgag agactctcat aacgtgccac taaataaaaa
     8281 tcgtacgtta ttttaaaaaa tgggttgatt ctaggcagta ctttgtcggt ctatttttag
     8341 aaaaagaggt tctttgtcaa acatacatga attcgcaagc tcattcaccc tgaaggatga
     8401 aaggcagaaa ctgtagtata ctaatggtgg aaaggaaaaa aagccgccag caatttggaa
     8461 aagaacgtta tgtcgattgt caaactggct tgctttattt ctcgagaacc ctgaaatata
     8521 gccaatgagc atgtgtataa aaaattgatt ttatatgaaa taaagcactc ctttcctaaa
     8581 attattggaa aggagtgcgt tttttatgga aattatttag gttctttgtc aattgctatt
     8641 gttttgctca aatcgtatgt taggaaatgc ttcggcattt ccttttttgc gttattaaat
     8701 gatttcacag atgttctgtt gaatcaggac acgcatccgg agatgacgaa aagtccgaaa
     8761 tccaaaagca attcttttga ttactttgat gaggttattc tttccctcca attttccgtt
     8821 tgaataggga tattttaagg agtttgtgat tgcttctgtg tgctttttca gataacaaat
     8881 tgccttttta aacccatcat tcaaggaatg tggcaatgat tcaatgagat tgaaaaattc
     8941 agtaaaatct tttgcatcaa aagcagctaa taactcttga tagacttcat agctttgtcg
     9001 tagttggtta tctatagaga gtaggtaatc aagcacctcc gactccgtca tgtatttccc
     9061 ttggaagagg ggaaattgct taaaagggtt ctgttgcgaa gtttgaaaat caaacgcgcc
     9121 ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat ttcagtagac
     9181 accgaaaagc cgaagagcgt tccatttctt cggttctttt tatatattcc tcgaatggtc
     9241 tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt atttcgtcgt
     9301 ttaataggtc gatggtcttg ttctattaaa ttgttaagat acttcacagt tcggtgctct
     9361 gtcttagtat ataaacccac actctgtaac tttctaaagg cggagccaag agaaggtgct
     9421 ttatcggtca caattgcttt cggctcacca aactgtttat ggagtcgttt taagaaagca
     9481 taggctgctt gcgtatcccg tttctttcgt aaccagatat ctaaggttaa gccgtccgca
     9541 tcaattgcac gataaagata atgccaacgt cccttaattt tgatataggt ttcgtccatt
     9601 ttccatgaat agaaggattg tctatttttc ttcttccaaa gataatagag gactttgctg
     9661 tactcttgca cccaacgata aatcgtagta tgacaaacat ttattccacg atcatataac
     9721 aattcctgaa cttcacgata gcttagattg taacgcaggt agtaaccaac agcgacaata
     9781 atgacgtctt ttttgaattg tttgccttta aaatgattca ttactctgtc ctctctgtct
     9841 tttttctcaa ttttacacta aaatagattt tttggaaaac tttgcaacag aaccatttct
     9901 aatatgaaag caattatcaa cagtaaggat tacgaaggat ataaagccaa acatccagaa
     9961 gcgaaaccat ttaaataccc acaagaaatg aagcgagcat ggcgtaaaat gttagatgat
    10021 gagttgattc cacttgaaaa tgaattgagg taaatgagaa gagttaagaa aattgtacga
    10081 ttcaaagata gtagtaaagt ggaagtgtag aaaataggga tagatgtctt ttatttgttc
    10141 acattttata acgaagtatt taagacagct attgaataat gtttcctcca cctttttcat
    10201 tcatttaccc caccccgttg ttatatgacc gtgtttcgag agaaaccaat aaaaagcata
    10261 ggctaactag gggttccttt ttcttttgat tttttggaat ccgcttaagt gaaatgtatc
    10321 tgaaatgaaa cagagataaa gtgatttttt gctgtaatga caaggtttta gctgtttcag
    10381 ataccatgta acccaaaata gtttattgag aaaataggag agtatttgat actaactagt
    10441 aggaaaaaat gagataaaaa gtaaatggaa tcatttcagg taatctcaaa taaaaaatat
    10501 acaggcctgt ggcactttac ctacgggact gtatattttt tactctcagc gggcttgttt
    10561 tgactccgtg tttgactccg tcgtattgat tttaaatgat attcgatgac caattaatag
    10621 gtcgtttttg aaatttttgt ctctatatga ctcagtttga taattattga tagatcgatt
    10681 tgggtctata tcgaacaagg gaagattgca aaagtcgaag cttaaaggat tgatattaaa
    10741 ggattaagag gtaaaaaggt atccccataa tatcctctga ctccgttttt gacctcgctt
    10801 atcatccgtt catgtaagaa tcaaataatt cgactgtttc attttgccga ttttttgata
    10861 ctttaatata aatgtccatg attgttttag aagaagaatg tcctagccta tcttgtacat
    10921 cttttacttg agcacctgct tcaaagagaa gggtagcatg agtctttctg aagccatgga
    10981 ctttgattat cttcatatct gggaacttcc gtgctacttt atccatgaca tggttagggt
    11041 agcctttgta aaggaagtcc ctttttttat tccctttgat agtgctgatt acaggtatat
    11101 catctatatt atctatccga atccctctag aaaggttctt ttatgttaca gaacgtttcc
    11161 attttttaga atacgtaagg ttccattatc aattgaaatt actccctttg atttgtctgt
    11221 ttttgtagga ttaatacata gagagccctc ggatagttct actaacgttt tgttgaagca
    11281 gatagtttga ttatcgaaat ctatgtctct acatgtcagt ccgagtattt ctccttttcg
    11341 ttcccctgta taagaagcta ttctaaagaa tactaaagca cgctcatctt ttaacttatg
    11401 caaacattca aaaaacagat atttcacata tatctaaaat tcggagattg aaagaatgaa
    11461 tttatctaag aattttcact aatgttgcga atataatatc agaaaatttc gaaataaatt
    11521 t
//
LOCUS       DADXTH010000083        11295 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.083, whole genome shotgun sequence.
ACCESSION   DADXTH010000083 DADXTH010000000
VERSION     DADXTH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.083"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWI97_002303"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWI97_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBC2463787.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWI97_002304"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWI97_002304"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBC2463788.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWI97_002305"
     CDS             complement(2169..2906)
                     /locus_tag="IWI97_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBC2463789.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWI97_002306"
     CDS             complement(2997..3524)
                     /locus_tag="IWI97_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463790.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWI97_002307"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWI97_002307"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBC2463791.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWI97_002308"
     CDS             complement(4401..4886)
                     /locus_tag="IWI97_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBC2463792.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWI97_002309"
     CDS             complement(5039..5236)
                     /locus_tag="IWI97_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463793.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWI97_002310"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWI97_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBC2463794.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWI97_002311"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWI97_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBC2463795.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWI97_002312"
     CDS             9211..9423
                     /locus_tag="IWI97_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBC2463796.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWI97_002313"
     CDS             9425..11104
                     /locus_tag="IWI97_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBC2463797.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3275 a         2437 c         1980 g         3603 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta ttttt
//
LOCUS       DADXTH010000084        11150 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.084, whole genome shotgun sequence.
ACCESSION   DADXTH010000084 DADXTH010000000
VERSION     DADXTH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.084"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWI97_002314"
     CDS             6..305
                     /locus_tag="IWI97_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2463798.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWI97_002315"
     CDS             378..1739
                     /locus_tag="IWI97_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBC2463799.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWI97_002316"
     CDS             1761..2021
                     /locus_tag="IWI97_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463800.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWI97_002317"
     CDS             2035..2787
                     /locus_tag="IWI97_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBC2463801.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWI97_002318"
     CDS             complement(2881..3486)
                     /locus_tag="IWI97_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2463802.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="IWI97_002319"
     CDS             complement(3542..4516)
                     /locus_tag="IWI97_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBC2463803.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="IWI97_002320"
     CDS             complement(4894..5613)
                     /locus_tag="IWI97_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2463804.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="IWI97_002321"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="IWI97_002321"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBC2463805.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="IWI97_002322"
     CDS             7164..8561
                     /locus_tag="IWI97_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2463806.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="IWI97_002323"
     CDS             8642..9082
                     /locus_tag="IWI97_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBC2463807.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="IWI97_002324"
     CDS             9229..9555
                     /locus_tag="IWI97_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBC2463808.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="IWI97_002325"
     CDS             9556..10305
                     /locus_tag="IWI97_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463809.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="IWI97_002326"
     CDS             complement(10331..10741)
                     /locus_tag="IWI97_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBC2463810.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3538 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
//
LOCUS       DADXTH010000085        11138 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.085, whole genome shotgun sequence.
ACCESSION   DADXTH010000085 DADXTH010000000
VERSION     DADXTH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.085"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            65..331
                     /locus_tag="IWI97_002327"
     CDS             65..331
                     /locus_tag="IWI97_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBC2463811.1"
                     /translation="MTSTYASFDKDNLYYDFPSVSGFSSMNNKKYLLMLTRLGYSTSN
                     VRAKRNGGTVITDILLSNQYQVIPNQEIQHQATSGVKIIESEVS"
     gene            413..709
                     /locus_tag="IWI97_002328"
     CDS             413..709
                     /locus_tag="IWI97_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463812.1"
                     /translation="MANNLHYVTNLESRQFDPCWHQIDAQTKELLLGWEENQVVRDFS
                     LNFIMIDYYNLATSMQRFPNYGMDIDSFKQKSFSGEIQNEESNNLVIPILYEPG"
     gene            719..925
                     /locus_tag="IWI97_002329"
     CDS             719..925
                     /locus_tag="IWI97_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463813.1"
                     /translation="MDGEEYPYKVAFDDLIQLSNLPKDSNMKIAGEFFAPGFLVGLVI
                     SSIATGCFLFLLFLDYIKKTEDIP"
     gene            complement(1247..1935)
                     /locus_tag="IWI97_002330"
                     /pseudo
     CDS             complement(1247..1935)
                     /locus_tag="IWI97_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
     gene            complement(1938..3569)
                     /locus_tag="IWI97_002331"
     CDS             complement(1938..3569)
                     /locus_tag="IWI97_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBC2463814.1"
                     /translation="MKKTFISTSVIVFSLFFSNGLLAKAQTIEEKIKEKDDKIEAIVK
                     NKESAENYLTDLDNKITQLEKEYKKVLVEKNTSEKKLTEINQKITALEERIRLRNKLI
                     EEQARNAQVNQKSDSIISVMVNAESLSDAVTAAIGMTKLISASNEIMQAQIDDKENLD
                     ELKKEAEKKVTDAEKETRSLEEKENDLIEAKLTQSIKINEISASLATEQSEKAKFENQ
                     KQEAEKRREEELKAIAEEKRKEAEAAAILKAQEAEAASIIFENQKQEAEKRREKELKA
                     IAEEKRKEAEAAAILKAQETEAASIIFENQKQEAEKRREKELKAIAEEKRKEAEAAAI
                     LKAQEAEAASIIKDQEKDVSQLADTNDIGIGQENTDTQKEEQIPDETSEQDSNSVNQN
                     EYQDNNSSSDNTQIQSPSSSGWGAPVANIIVTSPFGGRADPTGFSGSFHDGIDMGGTS
                     STPIMASRSGTVVQASFDGSAGNYIIIDHGDGYYSYYLHLSNYIATPGQSVSAGQTIG
                     TMGTTGNSTGVHLHFGIATSSNWSGFVDPAPFLGI"
     gene            3841..4248
                     /locus_tag="IWI97_002332"
     CDS             3841..4248
                     /locus_tag="IWI97_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBC2463815.1"
                     /translation="MNKTLRLMKPWDYFIITAVIMASFVPYLLFDTNNDENNNRESTD
                     VIAVVKIDGEVVDKFNLSQVNESFEKTYYPQKGKYNIIEVAPGKIRVKEDNSPDQIAV
                     RTGWISRPGEMSICLPHKLIVEILGVEENPLIY"
     gene            4427..5710
                     /locus_tag="IWI97_002333"
     CDS             4427..5710
                     /locus_tag="IWI97_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HBC2463816.1"
                     /translation="MVGVITVDNTYLTDVFNQYKLRENLLYQTWFVKSEKRLKAFKKV
                     RKYVQVLIKHVNNGTFPTDFKNSALEDIMNVIAEQTEIFKGAKHAFMWKPKLRIPDIY
                     ENRENQLAFSLCLDQILKANQEIKMLFAVNILAEKKIRGLGPAVANILYFLEPTIFPP
                     FNTAIVNGYNYLTGSKIRLGKWEDYFKLRDGMIELNCLGGLFSKDLGAISAFLFDIGK
                     LNYIVRENEEEYVRISESNTQKLIKQKLEKEDGKNVHQKIQYILATLGNEIGYHAWIA
                     SNDHNRIVDGNRLGDVSIPLLPETIYQLPNPLSRTIGLIDVIWFSKNGEPICAFEVEK
                     STSIISGMNRMDDLYSIHQENLMMYIVSPDQREEEIRAQFNRPHYMRNQNLHDHLRYI
                     LFTDLEKHANFMKKFGKDFTILAPISHYPSGELVR"
     gene            6421..7971
                     /locus_tag="IWI97_002334"
     CDS             6421..7971
                     /locus_tag="IWI97_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HBC2463817.1"
                     /translation="MYTNYNMNQLTLDLSTSFSPKENHVAVFIHELVEALQINDPYLF
                     GRPREYDLGAMMKVVLFAYTRETFTSRKIERLAEENLYARWLTQERVPTYRTIARFCV
                     SNDVQELTNKGLDQLTEYLRARNLIDDALFIDGTKILADANKYSFVWKKNTIRFDQMN
                     REKLLTMMTELKEAYALKQIPEGTTLTVDMIDELLTRMELRLDELENEVEATKRLSPN
                     PAKQQRRTLKSQVRKLNAKREKLLEHQTQFSIYGTRNSYSKTDHDATFMRVKEDHMKN
                     GQLKPAYNLQIATCNQFVLGYDVFQNPTDTKTLKPLLEKMKTAQKSPHYLVADAGYGS
                     ESNYRYIEDELPQHTALIPYGTMFKEQSKKWQTDDRKVMNWVYEPNEDFYIDPKGVRF
                     NFHAYRQRTDADGFVRDFKEYQAEKTDANQALIPEALTPKGNRRKITVNPSWEYHKEK
                     QKERLSTPDIQKIYGRRKVDVETVFGFMQACSGFTRYTVRGLEKVRKQTGLLITAINM
                     MKLTKIGT"
     gene            complement(8085..8264)
                     /locus_tag="IWI97_002335"
     CDS             complement(8085..8264)
                     /locus_tag="IWI97_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HBC2463818.1"
                     /translation="MQWLLLLLCPIMMIFMMRGMHSGSHNEKDDEQLKQDLENLKEQN
                     DILSSELSDLKSRLK"
     gene            complement(8267..8491)
                     /locus_tag="IWI97_002336"
     CDS             complement(8267..8491)
                     /locus_tag="IWI97_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HBC2463819.1"
                     /translation="MEALLNLLPLLLLLICPISMMFMHKGHGHAGHHHMHGEDHSKHT
                     GSENDIASLKIQTEQLKEEVATLKNLVKER"
     gene            complement(8475..9278)
                     /locus_tag="IWI97_002337"
     CDS             complement(8475..9278)
                     /locus_tag="IWI97_002337"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBC2463820.1"
                     /translation="MREPYDRVFISFGPFTIYWYAIFIVFGIIIGYFVANRRAKRAAL
                     PEDTIVDLLLYGLPVSIISARLYYVLFELPLYIDDPLSILKIWEGGLAIHGGLIGAVL
                     TGVIYCKKKNLSFWNVADVVAPSIVIGQIIGRWGNFMNQEAYGEIVDIEFLQSLKLPS
                     FIIDQMYIDGAYHHPTFLYESLWNIGVLIILLLVSRNRMFFGQIFLIYVSLYSVGRFW
                     IEGLRTDSLMLTANLRMAQVLSIVLLIGSILTYIYLKKSKEEDLHGSIT"
     gene            9434..10048
                     /locus_tag="IWI97_002338"
     CDS             9434..10048
                     /locus_tag="IWI97_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBC2463821.1"
                     /translation="MKKSMLFLLFFVFALSLAITLTSNFPGLLKINLFFQSDINFQNW
                     SRSQVNQDFRNLMGYLNNPFQKELVFDNLYVSDRGINHFKDVKFLFQLNYSLLLSTSS
                     VLLYLNRKKLVTRDQVREITSLIKWMIISVCVMALLFFDKAFVLFHQVFFDNDDWMFD
                     YRTDPIISFLPETFFFLCFLLIVTISVSTLTTIHHLFNKEERTL"
     gene            complement(10107..11138)
                     /locus_tag="IWI97_002339"
                     /pseudo
     CDS             complement(10107..11138)
                     /locus_tag="IWI97_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         3568 a         1946 c         1947 g         3677 t
ORIGIN      
        1 gacaatcaac caaactctaa cagaatttga agtatatcaa gaatattggg aaccactaaa
       61 aaaagtgact tcaacttatg catcctttga taaagacaat ctctactatg atttcccctc
      121 agtatcagga tttagttcaa tgaacaataa aaaatattta ctaatgttaa cgagattagg
      181 gtactcaaca agtaacgtcc gtgctaaacg gaatggtggt acagttatta cggatatttt
      241 gctaagcaac caataccaag taattcctaa tcaagaaatc cagcatcaag cgactagcgg
      301 tgtaaagatt atagaaagcg aagtgagtta aacctcagat attgcttctt tttcatttga
      361 cgttctagaa gtaacaaaag atgagttact ttatgtttat tttcccgtca acttggccaa
      421 taatttgcac tatgtgacca atttggaaag tcgacaattt gatccctgct ggcatcaaat
      481 tgatgctcaa acgaaagaat tgttgctagg ttgggaagaa aatcaagtag ttcgtgattt
      541 ctcattaaat tttattatga ttgattacta taatttggcg acatcaatgc aacggttccc
      601 gaattatggt atggacatag attcattcaa acagaaaagc ttttctggag aaattcaaaa
      661 tgaggaaagc aataatcttg ttatacctat tctctatgaa ccaggttagg tttttactgt
      721 ggacggggaa gagtatcctt acaaagtagc ttttgacgat ttgattcaat tgtctaactt
      781 accaaaagat agtaatatga aaattgctgg agaatttttc gcaccaggat tcttggtagg
      841 cttggtaatc tctagtattg caactgggtg ctttttattt ttactttttc tggattatat
      901 caagaaaaca gaggatattc cttaggaaaa tcacaatcta aatcttccct agaaactata
      961 gaatgacaag ggggatataa gcaaattcct aaatgattaa atagggtgcg ttactagtta
     1021 gctccatagt aagatttttt caaaattttc ttgaggttgg atcaaaaagg gctcttttca
     1081 attagattaa agacactcat acaaaataga gaaacgaact tataatcgcg ttcgtttccc
     1141 tatttaattt attatgacca taaaccaatt tgaatttaga aaacaccttc tttctttaat
     1201 aaaaagtact actcgtaatc tgtcaggatt gataagactt tttatattag ctaaataaag
     1261 aatggatata ttgattagca ttcttaaatt caactgtatc gatcagtgta ccttgtacaa
     1321 tcaatctcgt tacagcagcc aaatctccgc atgtaacaag agtgatcata tttttattag
     1381 gaatatcatc aatgacttcg actcttgatg gatccacttt ttctttaaag gacacttggt
     1441 aaatataaat attctttaag tctgttacat atattttttc ttttagagaa ataaactcta
     1501 atggggaaaa taaaatagtt ttatctttag cactatgact tgctaaagcg taatttcgtt
     1561 ttcccatctg ctgatctggt ttcattgttc cagccccaga aagaagcgag gtgttgtcca
     1621 atccgttgta tattggtaaa gataatttta cattgtgtat agcgatacca cctattatag
     1681 gcaaatcatt attcttgaat tgtgctttta atactaactc atttgatata gattcaacag
     1741 aatcaaaatc aaagtttcct ttccttttca gattgtcttc caaatctttc tgtgaataat
     1801 ttgctatgtc atattgtgga cttgtttatt cagtaagtaa gatcgaattt ggaaattaaa
     1861 tattaaaact aagcctataa gaaaaaggct aattagaagt aaatttaaag aaagatgctt
     1921 gatttttttc atcattttta gattcctaga aatggagcag gatctacgaa tccagaccaa
     1981 tttgagctag tcgctattcc aaaatgcaaa tgaactccgg ttgaatttcc tgtagttccc
     2041 atggtaccga tggtctgtcc agcactcact gattggcctg gtgtagcaat ataattactt
     2101 aggtgaaggt aatatgaata gtaaccatct ccgtgatcaa taataatgta attacctgcg
     2161 gatccatcaa atgacgcctg cactacagtt cctgatcttg atgccatgat aggcgttgaa
     2221 gaagttccac ccatatctat accatcgtga aaagatccag aaaatccagt aggatcagct
     2281 cttcccccaa atggacttgt gacgataatg ttggctacgg gtgcgcccca accagaagaa
     2341 ctaggacttt gaatctgagt attatcggaa gaactattgt tgtcttggta ctcattttga
     2401 ttaactgagt tcgaatcttg ctccgaagtt tcatctggta tctgttcttc tttctgtgta
     2461 tcagtatttt cttgacctat tcctatgtca ttggtatcgg ctagttgtga tacatctttt
     2521 tcctgatctt ttataataga tgcggcttct gcctcttgag cttttaaaat agccgcagct
     2581 tctgcttctt ttctcttttc ctctgctata gctttaagct ctttctccct tcttttttca
     2641 gcttcttgtt tttgattttc aaatataata gatgcggctt ctgtctcttg agcttttaaa
     2701 atagccgcag cttctgcttc ttttctcttt tcctctgcta tagctttaag ctctttctcc
     2761 cttctttttt cagcttcttg tttttgattt tcaaatataa tagatgcggc ttctgcctct
     2821 tgagctttta aaatagccgc agcttctgct tcttttctct tttcctctgc tatagcttta
     2881 agctcctcct cccttctttt ttcagcttct tgtttttgat tttcaaattt tgctttttcg
     2941 gattgctctg tcgccaaaga agcggatatc tcattgattt ttatactttg agttaatttg
     3001 gcttctataa ggtcattttc tttctcttcg agagatcgag tttctttttc cgcatctgta
     3061 acttttttct cagcttcttt ttttaattca tctaaatttt ctttatcatc tatctgggcc
     3121 tgcataatct cattactagc agaaataagc ttagtcatac caatggctgc tgtgactgca
     3181 tctgataatg attcggcatt taccatgaca gatattatag agtcactttt ttgatttact
     3241 tgtgcattcc tagcctgctc ttcgataagt ttattcctaa ggcgtattct ttcctcaaga
     3301 gcagtaattt tctgattaat ctcggtaagt ttcttctcag atgtgttttt ttccactagc
     3361 acttttttat attctttttc tagctgagtt attttgttat ctaaatcagt aagataattt
     3421 tcagctgatt ctttattttt tacaatcgct tcaattttat catctttttc tttgattttt
     3481 tcctctatag tctgtgcttt agctaagaga ccattactaa aaaatagcga aaacacaatg
     3541 acagaggtag atataaatgt ttttttcaaa ttgatttttc acttccctta ttttatttgt
     3601 gaagaaacat tttacataat attctcctct ctattttatc gttgctacag gttctactag
     3661 gaactgaaat tggaattact atacaaaaag taataggatc catatgctat atttcaattt
     3721 agtagacagc ttcaaaataa ctaaaactta tggagatttt atggagcata tacataccta
     3781 cataaaattc acacaactgt aaagtatgat atcaaaagtt aatacaggag aacgaattta
     3841 gtgaataaaa cattgagact catgaaacct tgggactatt tcattattac tgcggtcatt
     3901 atggcatcat tcgtaccata tttactattt gatactaata atgatgaaaa taataatagg
     3961 gaaagtacag atgtaattgc agttgttaaa atagatggag aagttgtaga caaatttaac
     4021 ttatcacaag ttaatgagtc ttttgaaaaa acatattacc ctcaaaaagg gaaatacaat
     4081 attattgaag tagctcctgg aaaaatcagg gtaaaagaag ataatagtcc tgatcaaatt
     4141 gctgttcgta caggctggat tagtagacca ggggaaatga gtatatgtct gcctcataaa
     4201 ttgattgttg aaattttagg agtagaggag aatcctttaa tttattagta tgaactaata
     4261 agtaccacga atcctattat agagacatgg acggagtaat ctaaggaaag accgttaact
     4321 aaaatcgcta aaacctgttt acatgattct tcatttaagg tggttacgga attttgcata
     4381 tagaaagcca tattagagct aacgagttta taaaatatta atagggatgg taggtgtcat
     4441 tacagtggat aatacttatt taacagatgt gtttaatcaa tataaattac gcgaaaattt
     4501 actgtatcaa acttggtttg tcaaaagtga aaaacgtttg aaagcgttta aaaaagttag
     4561 gaaatacgta caagtactta tcaaacatgt caataacgga acgttcccaa cagattttaa
     4621 aaattcagcc ctagaggaca taatgaatgt aattgcagaa caaacagaaa tttttaaagg
     4681 agccaagcat gcatttatgt ggaaacctaa attgagaatt ccggatattt atgaaaatag
     4741 ggaaaatcag ttagcttttt cactttgtct tgatcaaatt cttaaagcta atcaagaaat
     4801 taaaatgtta tttgctgtaa atattctagc agaaaagaaa atcagagggc ttggtccagc
     4861 tgtcgcaaac attttatatt ttctagaacc taccattttc cctcctttta acacagctat
     4921 tgtaaatgga tataattatc ttacgggctc gaaaattcgc ttaggtaaat gggaggacta
     4981 ttttaaactc cgtgatggta tgattgaact caactgctta ggaggacttt tttcgaaaga
     5041 tttgggtgct atctcagctt ttttatttga tattgggaaa ctaaattata ttgttcgcga
     5101 aaatgaagaa gagtatgtca gaatttcaga aagtaacacc cagaaattaa taaaacagaa
     5161 gttggaaaaa gaggatggca aaaatgtgca ccaaaaaatc caatatatct tagctacttt
     5221 aggtaatgag ataggttatc atgcttggat tgcctcaaac gatcataata gaattgtaga
     5281 tgggaatcgc ttaggagatg tgtctatacc tctattacca gaaacaatct atcaattacc
     5341 aaatccgtta agtaggacga ttggtttgat tgatgttatt tggttttcaa aaaatgggga
     5401 accgatatgt gcttttgagg ttgaaaaatc gacgtcaata atttctggga tgaatcgaat
     5461 ggatgatttg tattcgattc atcaagaaaa tttgatgatg tatattgtct caccggatca
     5521 acgtgaagag gagatacgag cacaatttaa tagaccacat tatatgcgta atcaaaattt
     5581 gcatgatcac ttacgataca ttctatttac agatttagag aaacatgcta attttatgaa
     5641 gaaatttggc aaagatttta caattttagc cccgatatct cattatccaa gtggtgaact
     5701 agttcggtag ttattctgga caaagaagtt gaaattaata cggttaattg tagtataaat
     5761 aagcatttgg gcagaattgg aaatcaatct tccctttttt tacgagtagc ttccacattt
     5821 ataatggcgc tcattgctat tgaatgtctt agtaacgtcc agaagaaaat catcatatta
     5881 atgctgccca tgcttccatt ttagtaactt ttcgttgaag aaaattgcgg atagagtgaa
     5941 tactatctat tcataaccta gaaatgttat ctaaaaaggt attttcctta tattcatcga
     6001 agataatggt tttatttgca cttaaaaaag aaacaataaa tcttacttct agaatcccct
     6061 ggtgtatgga ctaccctata caccagggga tgttatgtac tcttggtgga atgcaacgag
     6121 tggatggaat gatgatgatc attttctttg gtgtcagaaa tacataaaga aagattataa
     6181 accacgcatg ccttattcgt tttataaaga gtaaagttag actaaatatt ttgagagaat
     6241 caattctatt ctgaaccatg aatttagtga attttaaagg gactgtgaca taagtccata
     6301 gaagaaaacc gcaagaaatg acgagaaagc gttcatttct tgcggttttt tgggtaaaat
     6361 aaaaaggagc accggttccc gccaatgcac catcatttaa tcctgaaagg actaaacaaa
     6421 atgtatacta attataacat gaatcaactc actttggatc tatctacttc attttctcca
     6481 aaagaaaatc atgtcgccgt ttttatccat gaactggtcg aagcgcttca gattaatgac
     6541 ccctatcttt ttggtcgacc aagagaatat gatttaggtg ccatgatgaa ggtggttctt
     6601 tttgcgtata cgcgagaaac ctttacaagt cgcaagattg aacgcttagc tgaagaaaac
     6661 ctatatgctc gctggttgac gcaagaacga gttccgactt atcgaactat cgcccgtttt
     6721 tgtgtatcca acgatgtaca ggagttgacc aataaaggtt tagatcagtt gacagagtat
     6781 ctgcgtgcac ggaatctgat tgatgatgcg ttgtttattg acgggaccaa aatcttggcg
     6841 gatgcgaata aatacagctt tgtctggaag aaaaatacga tccggttcga tcaaatgaat
     6901 cgcgaaaaat tgcttactat gatgacggaa ctaaaagaag catatgccct gaaacagatt
     6961 cccgaaggga cgaccttgac cgtagacatg atagatgaac tgttaacacg gatggaactg
     7021 cgtctggatg aactagaaaa tgaagttgag gcaacgaaac gtctttcgcc aaatccagcg
     7081 aaacaacaac ggcgaacgtt gaaaagccaa gtacgcaaac taaatgcaaa gcgagaaaaa
     7141 cttctggaac atcaaacgca gttttctatt tatgggacta gaaacagtta ttcaaaaacc
     7201 gatcatgatg cgacatttat gcgcgtaaaa gaagaccaca tgaaaaatgg ccaacttaaa
     7261 cctgcgtata acctgcagat cgctacatgt aatcaatttg tgctcggcta cgatgtcttt
     7321 caaaacccaa cagacactaa aacactgaaa ccattactgg aaaaaatgaa gactgcacaa
     7381 aagtcccctc attatttggt cgccgatgca ggttatggtt cagaaagcaa ttaccgctat
     7441 atagaagatg aacttcctca gcatactgca ctgattcctt atggaacgat gtttaaagaa
     7501 caaagtaaga aatggcagac agatgaccgg aaagtaatga attgggttta cgagcccaat
     7561 gaggactttt acatcgaccc caaaggggta cgattcaatt ttcatgccta ccgtcaacga
     7621 acagatgcgg atggctttgt ccgagatttt aaagaatacc aagcagaaaa aacggatgcg
     7681 aaccaagcgc tcattcctga agccttaacc ccaaagggga atcgtagaaa gatcactgta
     7741 aatccgtctt gggagtatca taaagaaaaa caaaaagaac ggttatcaac gcctgacatt
     7801 caaaaaatat atgggcggag aaaagtcgac gtggaaacag tcttcggatt tatgcaggct
     7861 tgttcgggct tcactcgcta cacggttcga ggcttagaga aagtcagaaa acagacgggt
     7921 ttactcatta cggcaatcaa tatgatgaaa ttgacaaaaa taggaacttg aactactcaa
     7981 aatgaataaa aaaacaagaa gcactcgaaa aatctcatga tttccaagtg ctccttgttt
     8041 tttggagagc gtaactaggt aacttatgtc acgcctcctt taacttattt taaccttgac
     8101 ttcaaatcgc ttagttcact acttaagata tcattttgct cttttaaatt ttcaagatcc
     8161 tgtttcaatt gttcgtcatc tttttcatta tgcgatcctg agtgcatacc cctcatcata
     8221 aatatcatca ttatcggaca taataaaagt aataaccact gcattgtcat cgctccttaa
     8281 ctaagttctt tagagtagca acttcttctt ttaattgctc agtttggatt tttaaacttg
     8341 ctatatcatt ttcggaacca gtgtgttttg aatgatcttc tccatgcata tgatgatgcc
     8401 cagcatgtcc gtgcccttta tgcataaaca tcattgagat agggcaaatt agtagtaata
     8461 gtagtggtaa gagattaagt aatgcttcca tgtaaatctt cctccttaga ttttttgaga
     8521 tagatgtaag ttaatataga gccaattaat aggactatgg acaggacttg agccattctc
     8581 aagtttgctg ttagcattaa actgtcggtc cgtaatcctt caatccagaa tctcccgacg
     8641 gaatatagtg aaacataaat taaaaatatt tgcccaaaga acattctatt tctagacacc
     8701 agaagcaaaa taatgagtac tcctatattc cataaagact catataaaaa agtcggatga
     8761 tggtaggccc catcaatata catttgatca attatgaaag atgggagttt cagagattgc
     8821 aggaactcta tgtctacaat ttctccatag gcctcttgat tcataaaatt tccccatcgt
     8881 ccaatgattt gtccaattac tatactggga gcaactacgt cagcaacatt ccaaaaagaa
     8941 agatttttct tcttgcaata aataacccct gtcaatacag caccaattaa tccaccatgt
     9001 attgctagac caccttccca aatttttaag atacttaaag gatcatcaat gtacaaaggt
     9061 aattcaaaaa gtacatagta taatctggcc gatataatag aaactggcaa tccatataac
     9121 aacagatcaa caattgtatc ctcaggcaat gccgcacgct ttgctcttct attagcaaca
     9181 aaatatccga ttatgatccc aaacactatg aaaatggcat accaataaat agtaaatgga
     9241 ccaaacgaaa taaatactct gtcgtagggt tctctcaaat tcttccctcc ttcctaatga
     9301 acttgttttg actatagcag gctattatgg aaattttatg tagacttttt gacagataca
     9361 ttggtacaca ttttctacac aagttttttt atcattatat taaaaggagt gatctaatat
     9421 aaatattaat ttaatgaaaa aaagcatgct atttctatta ttttttgtct ttgctttatc
     9481 cctcgcgatt acattgacaa gcaacttccc tggtttgctc aaaatcaatt tattctttca
     9541 aagcgatata aattttcaaa actggagtag atctcaagtt aatcaagatt ttagaaattt
     9601 aatgggctat ttaaataatc cttttcaaaa agaattggtt tttgataatc tatacgtctc
     9661 agatcgcggt ataaaccatt ttaaagatgt taaatttttg ttccagctta actattcatt
     9721 gcttctttct acgagtagcg ttcttttata cctaaacagg aaaaaattag taacgcgtga
     9781 tcaagtcaga gaaatcacta gtttaatcaa gtggatgata atatcagtct gtgtaatggc
     9841 acttctgttt tttgataaag catttgtgct ttttcatcaa gtattttttg acaatgatga
     9901 ttggatgttt gactatcgta cagatccaat tatcagtttt ttaccggaaa catttttctt
     9961 tttatgcttt ttattaattg taactataag tgtttctact ttgacaacaa ttcatcattt
    10021 gtttaataaa gaagaacgta ctttatgaat agccgggagc gtcaataatt ttgtgtaaat
    10081 aactcgtcct tctgcaaaat aatgggttac tcagtaaaca ttgaagctaa tgtatccgtc
    10141 acttgttgga agcctttatg gcttctattt aagaattttt gattgtatgt atcaaagatg
    10201 gatactagga aacgctctag tgattcttca ttttgaaact gatcttttct gcggctgtat
    10261 ttcttgattt gtttattgaa agattcgatt aaattagttg agtaaatgct tcggcggata
    10321 ccaggcggaa actcataaaa agtcaataaa tcttgatttt ttatcagtga ctgcgtcact
    10381 ttaggataag ttttctgcca cttctcaatc atactcccta aaaaggtatt tgtctcttcc
    10441 tttgagctag cttgataaac agccttaaaa tcatcacaga tttcttttcg atctttgaca
    10501 cgtactttat gagcaatatt tcgggaaacg tgaacacagc aatgttggta tttagcttta
    10561 ggataaatct ggtaaatcgt atctttcatg ccttttaagc catcggtaat aaacagtaag
    10621 acatcatgaa cccctctgga gtaaatgtcc tataacaact cattccaaac gtaggtcgat
    10681 tcagtcggag caatcgcata acttagtact tctttagtgc cgtcttctcg tataccaatg
    10741 gcaatataga ttgtttcttt ggagacagtt tgccgtttta aaggaatata agtagcatcc
    10801 atgaaaatag cgacatactt atcatttaga gttctaactt taaatgcatt tacttcttca
    10861 gtcagcactt tagtcatgtt ggacatggtt tggggcgtgt agtgatgccc atacattttc
    10921 tcaatcaagt ctgcgatttc cgacatggta ataccttttc ggaagaggtg aatgacggtc
    10981 tcctctaagg tatcattcgt ccgtctataa gcaggaacag tctgttgctt gaactcgccg
    11041 ttgcggtcgc gcggaatctg gagatgaagt tccccgtact cggtcttgac cgtacggtca
    11101 taggagccgt tacgagaatt acccgtgtta aaaccaat
//
LOCUS       DADXTH010000086        10590 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.086, whole genome shotgun sequence.
ACCESSION   DADXTH010000086 DADXTH010000000
VERSION     DADXTH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.086"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(90..176)
                     /locus_tag="IWI97_002340"
                     /pseudo
     CDS             complement(90..176)
                     /locus_tag="IWI97_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            385..1667
                     /locus_tag="IWI97_002341"
                     /pseudo
     CDS             385..1667
                     /locus_tag="IWI97_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1976..2779)
                     /gene="lnu(B)"
                     /locus_tag="IWI97_002342"
     CDS             complement(1976..2779)
                     /gene="lnu(B)"
                     /locus_tag="IWI97_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HBC2463822.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            complement(2833..4317)
                     /gene="lsa(E)"
                     /locus_tag="IWI97_002343"
                     /gene_synonym="lsaE"
     CDS             complement(2833..4317)
                     /gene="lsa(E)"
                     /locus_tag="IWI97_002343"
                     /gene_synonym="lsaE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Lsa(E)"
                     /protein_id="HBC2463823.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTECMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            complement(4760..5320)
                     /locus_tag="IWI97_002344"
                     /pseudo
     CDS             complement(4760..5320)
                     /locus_tag="IWI97_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5394..5858
                     /locus_tag="IWI97_002345"
     CDS             5394..5858
                     /locus_tag="IWI97_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463824.1"
                     /translation="MMTMCPRCLELYSEIWSKPCCKCADKTIPVDIELINVVQMLLTR
                     GFDVSYATCYPDKEQGEIEAMEIEIHFRELYPQALFDGLPPDWIVIDEYPVLGGKVLD
                     EPVDILTCAIEYRFEESIHIQKDIAISNLETWLEEKDPQSCRAILTLAVSAP"
     gene            5926..6120
                     /locus_tag="IWI97_002346"
     CDS             5926..6120
                     /locus_tag="IWI97_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463825.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            6110..6463
                     /gene="tnpB"
                     /locus_tag="IWI97_002347"
     CDS             6110..6463
                     /gene="tnpB"
                     /locus_tag="IWI97_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBC2463826.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            6565..8112
                     /locus_tag="IWI97_002348"
     CDS             6565..8112
                     /locus_tag="IWI97_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBC2463827.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(8256..8507)
                     /locus_tag="IWI97_002349"
     CDS             complement(8256..8507)
                     /locus_tag="IWI97_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463828.1"
                     /translation="MEYADDRKIQPDRITYRLIQEYIEEKYGFKVHTANIAEVKRNLG
                     LPMYDAPNAVEELKRPYKPAPEYKVEAIKDALRHFKVID"
     gene            complement(8725..9534)
                     /locus_tag="IWI97_002350"
     CDS             complement(8725..9534)
                     /locus_tag="IWI97_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(9) family aminoglycoside
                     nucleotidyltransferase Spw"
                     /protein_id="HBC2463829.1"
                     /translation="MSIDLSNKKIPKEAIQALKTIAELLDNMLIGVYLYGSAVMGGLR
                     MNSDVDILVITNQSLSEKTRRNLTNRLMLISGKIGNIKDMRPLEVTVINQKDIVPWHF
                     PPKYEFMYGEWLREQFEKGEIPESTYDPDLAILLAQLRKNSINLLGPKATEVIEPVPM
                     TDIRKAIKESLPGLIASINGDERNVILTLARMWLTASTGEIRSKDLAAEWAIPQLPDE
                     HATLLNKAREAYLGECVDKWEGMESEVAELVNHMKKSIESSLNIQLPFRIV"
     gene            complement(9666..10193)
                     /locus_tag="IWI97_002351"
     CDS             complement(9666..10193)
                     /locus_tag="IWI97_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBC2463830.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            complement(10237..>10590)
                     /locus_tag="IWI97_002352"
     CDS             complement(10237..>10590)
                     /locus_tag="IWI97_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBC2463831.1"
                     /translation="KEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYIN
                     KYIDEDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITR
                     YTEDMYKKYVENDYF"
BASE COUNT         3353 a         1992 c         1723 g         3522 t
ORIGIN      
        1 acaatagtta ttaaaatatt attataatca gcaagcaaaa gaaaacccac aactctaccg
       61 cagagtcatg ggctatttat ttctggtttt catatttcca tttcaatgcc agatttaaag
      121 caaattacaa agtggtcttc atagactgta acgctctgga ttatcttcct tacaagctta
      181 tcatcatatt ctaaggtgcg gaatttgttt ttgcggataa attcaggctc tataatttat
      241 aggactgatg aatcattttt tgattcatca gccctatttt agtttattta agcagaaaaa
      301 catgtcgtct atctagtaaa attaaagtac caaccaaaat cttaaataga aaggacgaca
      361 tgtcatgact aatcatatca aaaaatgttg cgaattaaag atgaagattt aaatttaact
      421 tgtgttgaag aaggaaaata caaaggtgtg aacacattaa ttatcactgg tacctactca
      481 cctaagctta cggcctgtaa gtattgtcag tctcatgcga acgatgcaga tgaaaagaaa
      541 accattgtaa aaaatgggaa aaaagaagtg acaattcgtt tggaatcata tcaaaatatt
      601 ccaactattt taaagctaaa aaaacaacga tatttttgta aaaactgcca tcaacattgg
      661 acaactcaat gttctatcgt tgaagaaaat tgttttatta gtcgattcat gacatttaga
      721 atactagaat tattaactca aaaaatatca atgaccgtta tcgctaagat atgtcatgta
      781 tcactcacaa ccgttataag ggtccttaaa tctgttgaaa aacatttacc tgaaacgctt
      841 aaaccacgct cttttccaaa agttctaatg gttgatgaat tcagatctca tgccacctat
      901 gaagataaaa tgagttttat ttgtgccaat ggtgaaaccg gtgaactggt tgatgttctt
      961 cctagtcgta gacttgataa actaatgaat tacttcaatc gttctcccaa ggagaaacga
     1021 gaacaggtaa aatttctagt gacagatatg aatgcggctt attttcagtt aactaaaaaa
     1081 atctttcctt gtgcagcttt agtcattgat aggtttcata tcgttaaaca cttaaaccaa
     1141 gcttttaatg actttagagt cagggaaatg aaagcattaa taaagaatca ccagcgttct
     1201 gaagccaata agctaaaagt aaattggaaa tacctattaa agaatcaaat gactgtttct
     1261 agttcagaat ataaaaattg gagaagcttt ccttcaccta agtttccccc tctaacagaa
     1321 tcaatgatga ttgatcgtct tttatcattt tctacagact taaaagaagc ctatggcgtg
     1381 tttcaattat taacctatca ctttagaaat aaagatccag actcattttt tgatttatta
     1441 aagaacttac cgactacctt agatccacta tttaaacaga agattgaaaa tctgattaat
     1501 tatgaagaag ggattcgtaa tgcttaacgt ataagtattc taacggaaaa atagaagcta
     1561 aaaatactca tattaagata ttaaaaagag tatcttatgg cttcaaatct tttagtaata
     1621 tgaagataag aatttttatg ataaacgacc ttgtcacaat tgaataacta aaaaaagatg
     1681 atgaatttga gaaaattctc aaattcatca tcttcaatat ttgtctcatc agtccgattt
     1741 gacaaagagc ccgttttttt atgttttcct tacttactta aaacagtcac acattcaata
     1801 tggattgaga ggacagaatg aatgtcattt gtgtatgtcc gttctcggaa acatatccac
     1861 gacacttttt gcactatcgg tagacgggaa catatccata ggcttcgctg ttttttgaaa
     1921 aactgaggta tgctgttaat tttcagtacc caatactgtg aataacgtta ctctcctatt
     1981 cactaatgta attcaacaat ctttctaaaa tgtcatgtgt aacttttaag ttgtattgtt
     2041 caataaggtg actttgcaaa tccataacta atagcaaaga atttttatag gcttcaaata
     2101 attctacctt atctaatcga gcagtggtct ttgcaaattt tttataattt tcaaggctaa
     2161 tttctttttc aaggttttta ctcatgttta gccaattatc agcatttttt tctgccatac
     2221 gtataagttg tagtgtattt ttttgaagtt gtgataagag ttctaatgaa cgagcatatt
     2281 ctcctctttt tagaacgttg attcccatca accatagatt agagaaatta cacaacaaaa
     2341 aattagcatt ttcttcagta agtctatttg gtcttgcacc acttatctct gataaataat
     2401 tttctaattg ccctgtttca tcgtaaataa gcatagcctt cgtatcagga atataacctg
     2461 aatctttaaa cgaggggatt atgttcatat ctttttcaga aaggaaatga aattccccac
     2521 gtataagatt atcaaaaata actacctctg ttccgtactc atttttataa agcatcaagt
     2581 acggagctac gtcaaacaac cagttggatg aatcaaagtt cgaggttata ctatctttca
     2641 aaaatatata gaactctata tcagagtatt ggtcaccttc tcctttggta aacgatccat
     2701 acatcataca agctgtaatt cgttcatctg actcagtaag attcttaacg tttgcaatta
     2761 attctttttg ttttaacatt tcttcaccct ttcatgtaaa tatcttttag ttattgtggc
     2821 aactcaaaat ctctacaaac ctaaattaat atttttcgta caaatgttat tgcaaaaagc
     2881 atcatcatgc tccacaaaca atagtgtagg acaatattcc aaaatcattt tttcaatttg
     2941 gatacgtgaa aaaatatcaa tatagttcaa tggctcatcc catatataca aatgtgcact
     3001 ttcacaaagg cttttagcaa ttagtacttt ctttttctga ccagcactaa aatccaccat
     3061 gttcttatca aactgctctc tattaaaatc cagtttacga agaatcgttt taaatagagt
     3121 ttcatcgatc ttattattat aggcaaattc agatagatta ccttttaaat atgaagtatc
     3181 ttgcgaaata taagaaattt ttagtccact tgctagcata aaatttccgg taaatttaat
     3241 atcatctcca ttaatcaatt ttaggatact agacttacca ctcccatttt ttccgataat
     3301 ggcaactcta tcaccaatat tgactctgaa attaagatta ctgcatactt ctttatctcc
     3361 ataagacaat gataaatcat tcgcttctat taagcactct ttgtgaaatt caagtggtga
     3421 aatttttaag tcatcatatt gttcaatgtt gtggagcagt tctgattttt gtaaaacggc
     3481 ttcctgatgt cttgactcaa tatttttggc acgtttcatc gcttttgcag ccttatgtcc
     3541 aacataaccc ttatccagtt ttgaaccaga atttgttgtt ccatatttac ttttttctac
     3601 tttatttgac cagtttgaac tacgttttgc tgcataagac aaccttccta tttctttaag
     3661 gagtttcttg ttttctgcca gttcaaaatt atcttgtaac gttttgttct cccaccaaga
     3721 agtaaaattt cccttttgga tttcgatatt cgttttattg atagatagta tatggtcaac
     3781 acattgatca agtaagcttc tatcatgaga taccaaaata aaccccttct tgcgtttcaa
     3841 atagttttgc actacattac gtgcatcgat gtcaagatgg tttgtaggtt catcaataag
     3901 caggaaacaa ctcgctgtaa ggaataaagc tgcaagaagg acctttgttt gctcaccatt
     3961 tgacaatgta ttaaacggac gatataaagc atcttcttga acatcaagca atgatatttc
     4021 acgaaaaatc tcccaatcca tacattccgt acaaatactc ttcattactt caattgtata
     4081 tagactctta tcttccacat cataaggaaa atactcaaac ttaactgtag aacttatatt
     4141 gccggaatac gcatatttgc caagcagtaa atttaagaaa gtagttttac cgcgtccgtt
     4201 tcttccaata aaaccgagtt tccaatctgt atctatctga aaacttacat tttcaaaaat
     4261 attgtcataa cttccttcat atgaaaaagt tagatttgaa acatttatta aggacatata
     4321 tttcttcctc ctttacatta taaaaatttt ataagcaaag tcggttttcg gcattaatta
     4381 ctctataatg ttatccaact ctgcaaggat tttgtcatga atgacatagt atcattgtct
     4441 atcacgttta tcttcctttc ttttacaaat attaaaagcc gcaagaaagt aaattcttac
     4501 ggctcaaaaa caaatactat tattaccttt tgttatacat aacataaggt tactgtaatg
     4561 tattattgag tgtagaatac ttaactttct tgcaatttgc ataacaaaac aaacggttct
     4621 atagccgttt ctttaaatat cttattatgc atcatataat tagcaagaaa agttatacat
     4681 tcttccacct ccaagtcgtt ttattatatc atattatctt taaaaatcca atataattct
     4741 tctgttctct ttctaaagat tacccttcta catcaatctc gactcctgat ttgaattcaa
     4801 cggcgaactt atcatgaaat accgtaactt tttctataag cctccttact aactgctcat
     4861 catattcctc taactcacag gattgttcat taaagaaatc agtcatttca gcgattcgtt
     4921 gcctttttcc ttcacgctct gcattttcaa caagtgcatt ttgcttcaat tctcgaaggt
     4981 ggtagatttc atcagccaca tcttcatagt cattcttgga ttttgcttgg ataagaagct
     5041 gttgttgtaa ttcttccaat ttgctatcaa tatcattggt ggcattatca ttttcttcat
     5101 taagtatagt agctatgttt ttctgcaacg cctggaggaa gggttctttg ttagccaaaa
     5161 gttcgttaat agccttaacc actgctgtct gcaatgtttc ctcgtttatg gtaggggcag
     5221 tacattcaga tcctttttcc tccaaacggc tgacgcatct ccaaacaata gacttgtaac
     5281 ctcggttatt ccagtgtacc cggcggtaaa tatcaccgca ctggcgagca aactgcaact
     5341 gcaactatgc aggcacttaa tcttaaaaga actatggtga aggaagtgat tgtatgatga
     5401 cgatgtgtcc acgatgttta gagctataca gtgaaatttg gtcgaagcct tgttgtaaat
     5461 gcgccgataa gaccattcct gttgatattg aactcatcaa tgtcgttcag atgctgctca
     5521 ctagaggctt cgatgtatct tatgccactt gttacccaga taaagaacaa ggtgagatcg
     5581 aagctatgga aattgagatt cactttaggg aattgtatcc gcaggctctc tttgatggtc
     5641 tcccacctga ctggattgta attgacgaat acccagttct tggcggtaag gttcttgatg
     5701 aacctgttga tatccttaca tgtgcaatcg aatatcgatt tgaagagagc atccatattc
     5761 aaaaggatat cgctataagc aatctggaaa catggctcga agagaaagac ccgcaatctt
     5821 gtagggctat ccttacattg gccgtaagcg ccccatagaa cagtacctta ctaacaaatg
     5881 cacctcaaga tatacttgag ctatcaagaa gtctggaggt tttctatgta taaattaaat
     5941 gagattgttc ctgtccaatt aggaaacgaa aagagtgcac cagaaaaagg taaacccgcg
     6001 gctgccaagt tagcgatgcg catcaaaaga gagaatggtt ctgaagtttt gatctataac
     6061 ggtattaaca attatattct tcaagctatt ttgaaagaac tgttgtccaa tgctttatga
     6121 tttacttcaa accaaacata tttatattgt gtgcggaaaa acagatttgc gaaaaggaat
     6181 tgatggtctt gccagtctta ttcaacaaga gtatcaactc gagctttacg aagatgccgt
     6241 ttttttattt tgcgggaacc gtcaagaccg tttcaaactt ctctattggg acggtgatgg
     6301 ttttcttctc tgttataagc ggatagaaaa cggcaagtta aaatggcctc ggacgaaaga
     6361 cgaagttcga accttaacga accaacaggt tcgatggttg ttagaaggat taagtatcga
     6421 tcaacctcga gctattttgc caggaaaaaa aggagttttt taagctgggg ggttaacaca
     6481 aaaaatgtgt tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag
     6541 ttcgattcaa ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa
     6601 gaagcccata aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc
     6661 caactattga atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag
     6721 actctctttg aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta
     6781 agtgttccag aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga
     6841 acgaaagcag acaaaatcaa acacttacct ataacagaga aagaacatct ccttcctttg
     6901 gaagaacaat tttgtgaaca ttgcggttca cagatgaaag atatcggacg gacaaaagta
     6961 cgagaagaaa ttcgtttcca tcaggctatg ttggattgtc tcacccatta tcaacatacc
     7021 tattgttgta aaagctgcga aaaagaagga ctttcttctt ttaaaaaagc gatcgttcct
     7081 aaacctttga tttctaatag cttaggttcc aatagtttag tggcggaaac gatccggatg
     7141 aaatttggtc agaaagttcc tgcctatcgg caagagaact attggaaaca aacacatggg
     7201 ttggatattt cgagagataa tatcaccaat tggcatatta aagcagtgca aaacgcgtta
     7261 gaccctttag gagaacgatt aagagtctat cttaatcaag aagagattct acacggggat
     7321 gaaacaagtt atcgtgtgat tgaaagtgcc aaaacagata cctattactg gcagttttgt
     7381 acaggaaaag acagtcagca tcccatcgtt tattatcacc acgacgaaag tcgtgctgga
     7441 gatgttccga aaacttttct caaggagttt acagggtatt tacactgtga cggctatagc
     7501 gggtataatg ccgtggaaag tgtacgactt gtctattgtt tcgcgcatgt tcggcggaaa
     7561 tttttcgaag caattccaaa aggaaagaaa aacacggata ttcccgcagc tcaagcagtc
     7621 aaacaattgg ataaatggtt tgtcctagaa aagaaatgga aagacttttc tcctgagaag
     7681 cgattaagct gtcggcaaca agaactgcgt ccattattta ttgcttttta tgagtggatg
     7741 gcaacgatag atcctgttgc aaaatccaag ttggatgcag ccgttcaata tgcgtgtaaa
     7801 ctacgaagtg gttttgaacc tatttttgaa gatggtcgtt tagaactcac aaataatcgt
     7861 gcggagcgaa atatcaaaga gctggtgata ggacggaaaa attggttgca ttccacaagt
     7921 cttgaaggag cacgaacttc aggaatcatt ctgagtgtat ataaaacagc agaacttaac
     7981 ggattgaatc ctgtaaagta tctagagttt ctatttgata agataccaaa tcttcccgta
     8041 ctttcagcag aaactttaga tcaactgttg ccttggaaca aggatgtaca acaacatttt
     8101 tctcgtaact aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg
     8161 tacaggtact gttctatggg gcgcttacca ttggccggct tctgaaaaag taagaccctc
     8221 tcaacagtaa tgatctgctg aaagggtact ttactttaat ctatgacctt gaagtgcctt
     8281 aatgcgtctt taatagcttc tactttatat tctggtgctg gtttatatgg gcgttttagt
     8341 tcttcaactg cgtttggtgc gtcgtacatc ggcaggccta agtttctttt gacctctgca
     8401 atgtttgcag tgtgaacttt gaagccgtac ttttcttcga tatactcctg aataagcctg
     8461 taggtgatac gatctggctg gatttttcgg tcgtctgcgt actccatttt tacactgata
     8521 aaatcgtctg cacttttgtg ggacaataaa acacacgtct caacgtggct cgagttgatg
     8581 gcattgatgt cttaagctga tttttaaccc tttcgttcgc gggaacgtat caacggtatt
     8641 ttgattattt tttaagaaac tgatatatgg gggaacatat cgaagcaatc aataatgcac
     8701 attaacaact agtctttgtt atatttaaac tattcgaaaa ggtaattgga tattaaggga
     8761 agactctata gactttttca tatgattaac gagttcagcc acctcagatt ccattccttc
     8821 ccacttgtca acacactctc ctaaataagc ctctctcgct ttgttgagta aagtagcatg
     8881 ctcatcgggt aattgaggta tcgcccattc agctgccaga tcttttgagc gaatttcacc
     8941 agtagatgct gtcagccaca ttctggctaa agttaaaatc acattgcgtt cgtcaccgtt
     9001 aatgctagct atcaacccgg gcaacgattc tttaattgct tttcgaatat ctgtcattgg
     9061 cacaggctca attacttctg ttgcctttgg tcccaaaagg ttaatactat tttttcttag
     9121 ttgtgctaaa agtattgcta aatccggatc ataagtcgac tcaggaattt ctcccttttc
     9181 aaactgctct cttagccact cgccatacat aaattcatat ttggggggga aatgccaagg
     9241 gacaatatcc ttttgattta tgaccgtaac ttcaagaggc ctcatatctt ttatgtttcc
     9301 tattttccca gatataagca ttaacctatt tgtaagattc cttcgagttt tttcagataa
     9361 actttgattt gttattacca aaatatctac atcgctattc atacgtaaac cacccattac
     9421 tgccgaacca tacaaataca ccccaattaa catattatca agtagttccg ctatagtttt
     9481 taatgcttga attgcttctt tcggaatttt tttgttactt aaatctatac tcaacttcga
     9541 tctcctttcg tctgaaaact gttttttaac gcctcttaaa atttataaaa aaagagtaaa
     9601 catctttcgt tgtattgtaa attaacgcca tccaaaattt tgattcaaga ttttctttcg
     9661 gtttattata tgtttgagcc aatataagct acaaaactgc tgtctatcca tttttgagta
     9721 acctcattta tatcaatccc aatagtagcc aacccaataa tgctgcaatc caatttctct
     9781 aacaaagcta ttaaagcctt catttgagaa cctgtctcta tccaatcatc tacaatcaaa
     9841 actcttttgt tcctcacaat agaactctta gatatttcaa agcttttatc ttgacctgaa
     9901 tagtcggtaa aatgtgtcga aataatctct tcttttgcgt atggcagctt atcacctttt
     9961 cttactccaa taaaccccac tccaagttct ttggctatgg cagtgcctag tatccatcca
    10021 attgcttctg gggcagccac ataatctact ttattttgaa aatccaatga tagtactttt
    10081 acaattttat caaaaacggt tttatgtgta aaaatagtta gtaaatcata ttttccaatt
    10141 gagttacgag gtaatatacc tctaatttca tttataacat cagtactttc catacattta
    10201 caccccttgc ttatggttaa taatctttgc taatctttaa aaatagtcat tttcaacata
    10261 ttttttatac atgtcctcgg tatatcttgt tatgttctta ccatactctg gataatcaaa
    10321 ccccagtagt tctgctacct ctttggacac ttccctgaac aattggtggc atataaataa
    10381 tgacttccaa atattttcat aggaatccat gcgatatgta gataataatc tattccatag
    10441 atcttcatca atgtatttgt taatatactt ataatttttc ccaacactta atgaaaattc
    10501 tgtctttatc ccaaccttcc acgacatcat cctaagtagt tcaaaccgta gaatctggtt
    10561 cagatgatcg attgcaaaca gtatctcttt
//
LOCUS       DADXTH010000087        10190 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.087, whole genome shotgun sequence.
ACCESSION   DADXTH010000087 DADXTH010000000
VERSION     DADXTH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.087"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(41..283)
                     /gene="acpP"
                     /locus_tag="IWI97_002353"
     CDS             complement(41..283)
                     /gene="acpP"
                     /locus_tag="IWI97_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBC2463832.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(405..1406)
                     /gene="plsX"
                     /locus_tag="IWI97_002354"
     CDS             complement(405..1406)
                     /gene="plsX"
                     /locus_tag="IWI97_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBC2463833.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1455..3491)
                     /gene="recG"
                     /locus_tag="IWI97_002355"
     CDS             complement(1455..3491)
                     /gene="recG"
                     /locus_tag="IWI97_002355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBC2463834.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3608..5290)
                     /locus_tag="IWI97_002356"
     CDS             complement(3608..5290)
                     /locus_tag="IWI97_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBC2463835.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5342..5704)
                     /locus_tag="IWI97_002357"
     CDS             complement(5342..5704)
                     /locus_tag="IWI97_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBC2463836.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5974..6162
                     /locus_tag="IWI97_002358"
     CDS             5974..6162
                     /locus_tag="IWI97_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBC2463837.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6400..9069
                     /locus_tag="IWI97_002359"
     CDS             6400..9069
                     /locus_tag="IWI97_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBC2463838.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9402..10148)
                     /gene="truA"
                     /locus_tag="IWI97_002360"
     CDS             complement(9402..10148)
                     /gene="truA"
                     /locus_tag="IWI97_002360"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBC2463839.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2767 a         2200 c         1835 g         3388 t
ORIGIN      
        1 ctgcatgcag ctttttatcc ttctttgctt ttatttgaaa ttatttaaga tggctgctga
       61 tataatctac tgcgccaccg actgtttcaa tttgttcagc gtcttcatct gaaatttccg
      121 taccgaattc atcttcaagc tctaaaacga attccataat actaattgaa tcagctttta
      181 aatcatcttt gatgttcaat tcatccgtca ctttatcggt atctatctca aaatgatttg
      241 aaataatttt cgctactttg ttaaatactt cttcgcgagt caactttttc acctccaaga
      301 atcatacact tgtcaattac ctacaccatg gctaatttta ctggcatttt tcaggaatgt
      361 ccaccttttt ttcaatggaa cggatcgctt cagatatcca aatactattc ttcgcttttt
      421 tcaaattgtt cgactaattg tccaactaca tctgtctcaa gcattgtgtg gatttggcgg
      481 atcgtatagc gaacagcatc ggggcctgtt gctccatgag ttttaatgac tggtgctttc
      541 aagccaaaca aaacggcacc gccatgtttg gaataatcca tttctgcttt caaagaacgt
      601 aagccgtctt ttaaaagcat agcgcccatt tttcctttga tcccttcatt tagaatagca
      661 gattttaata gattcatcat attcattgct gttccttcaa tagatttcaa aacagcgttg
      721 cctgtaaatc catctgtgac cactacatca gccacaccat tgagtagctc acgagcttct
      781 acgtttccaa taaaattcaa gcttgtttca ttctgaagga gttcataggc ttttttcgtc
      841 acttcactac cttttgttgc ttctgttccg ttgtttaaga gtgcgacacg aggacgtccg
      901 atacctcgga ctttacgtgc ataaaaagaa cctaaaatac cgtattgcaa caaatgttct
      961 ggtttgtttt ctgcgttagc gccaagatcc agcatatcga aaccggcacc ttctttccct
     1021 actactggca aggtagacat caagccaggg cgttcgatat ttttgatacg tcctacgatg
     1081 aacaaccctg ctgctagtag cgcgcctgta ttccctgctg aaaatacagc atctgcttca
     1141 ccgtttttca ctgcttgtgc agcaaggacc attgatgcag tcttttttcg tcgaattgct
     1201 tttactggct catcatcact attgattttt tcgtctgtat ggataatcgt gatgtttttt
     1261 tcatttgtta agtatttctt gatttctgct tcttttccgt aaagtaagaa ttctacttct
     1321 ggaaaatctt gctgcgctaa agccacgcct tctacaattg cttgaggtgc gtgatctccg
     1381 cccattgcat ctacggcaat tttcattgtt tccactcctt tttccgtcat cagtataaag
     1441 tatagcacag tctgctaatc gaaataacga ttatcctgat tttctatttg gaggctttgc
     1501 gctaaaggct gatactcagg aagcatctgc caatccttta tttgccaaag ggctgcagcc
     1561 tcttctctgg ctacctccaa aacatttcca tccgtcacca agtcagcaat cgcaaattga
     1621 ggcagaccag attgacggaa gccaaatact tcaccaggac cacgtaattc cagatctttt
     1681 tcgcttagta caaaaccatt ggtggtctct gtcatgattt tcatccgttc cacacctagt
     1741 tcatttttag gattagcaac taagatacag taggaagcat cacttccgcg tccaacacgt
     1801 ccacgaagtt ggtgcagctg tgccaatcca aatcgatccg catccatgat cagcatgacg
     1861 gtcgcgttcg gcacgtttac ccctacttca attacagtag tcgaaactaa tatctgcatc
     1921 tggttgtctt tgaaggcttc catgattact tctttttcct gattcttcat tttaccatgt
     1981 aacaagccaa cttcatattt tggtgcaaat agttcacgaa gtttttcata gatttccact
     2041 gcatttttta catctaatgc ttctgattct tcaatcagcg gacagatgac atacatttga
     2101 tgtcctcttg ccagttcttt tttcgtccaa tccagtacac tgtccaattg aggtgttctt
     2161 acccagcgtg tttcgatcgg gatacgtcca gctggaagtt catcaatgat agatacatcc
     2221 atctcaccat atgctgtaat agctaatgtt ctaggaatcg gagtcgctgt cataaataag
     2281 acatctggtc gccagccttt ttcacgaagt actttccgct ggttcacgcc aaaacggtgc
     2341 tgctcatccg taataactag acctaaattc tgaaattcga ctccatcttg gatcaatgca
     2401 tgtgtgccga tcacgacgtt aatttcccct tgctctagtt tttcaaggag ctcacgtcgt
     2461 tcttttgctt tcgttgaccc cgtaatcaat gcgatggtta cttcattggg atcaaacagc
     2521 tgttgcaagc tttccatatg ttgttgtgcc aagatttctg taggcaccat caacgcacct
     2581 tggaaacctg ctgtaacggt agcgtataac gcaatcgcgg caaccactgt ttttccgcta
     2641 cctacatctc cctgcaaaag tcgctgcata tgctgcggac tgcgaagatc ccgacagatt
     2701 tcattcgtta cacgtttttg tgcatttgtc agttcaaaag gtagcttttg agtaaattgt
     2761 ttcaattttt ggacatcata aaggatctcc aaaccattcg tttctgcttt ttctgctttt
     2821 ttcaatcctt gaagacgcat ttgaaataaa aagaactctt caaaaacgac acgtcgtttg
     2881 gcttgatggt tttcttcgtg gtcttttgga aaatgcatac ttcttacagc agaagctcgg
     2941 tcaagcagtc gatatttctc cacaaggttg tttggcagat tttcttcaat caatgtgccg
     3001 tattcttgaa accctttttt gattagatca actaatgtcg tttgacgaat tgatttgttg
     3061 acgtgatata tcggcgaaaa gtcatccatt gtttgagaac ctaaaatctt catcccgttc
     3121 aaggctttac gctttgcatc ccatttaccg tatacggcga tttcctccga taaaatgact
     3181 ttttctttta gataaggttg attaaaaaaa gaaacgttga acacatcatg ttcttgcatc
     3241 atacgaaatt gcaatcggct ttttttataa ccaaatcgat tcatcacagg aggcgagact
     3301 acgattcctt tcaaagtaac cttttcttga tcctggatct cgtttagatt tctttcctga
     3361 atatcttcgt agcgaaaagg ataataagtc agtaagtctt caatcgtttg gatgcccaag
     3421 ctggcaagac tagctgctct tttttctccg actcctgcca gcatagtgat tggatcatct
     3481 aaagaacgca taaatttttc ctccctcctt taacaaaaaa aggacttggt cagcgatcat
     3541 acctgatcac taaccgaagg ccttttatga atgaatactg tgttagaaag atattctgac
     3601 acagaaatta ttcagcagaa aatagataag gatagacagg ctgatctcct tggtggatct
     3661 cgatttccaa atcaggatgt ttttctgtta gtttctcagc aaattcattt gcttgttcaa
     3721 ctgttccttc atcacctgcc aagatcgtta cgatctctgt gtcctcattg atcatttgtt
     3781 ctagtgtact taacgctgtt gctgtgatat ctgggttaga gatcaggatt tttccatcga
     3841 tcatgcccaa aaagtctcct tctttgatct ctacaccgtc aattgaggta tctctgatcg
     3901 cgtgagtgac ttgaccgctc acaacgctag acaacatttc tgtcatcgtt tctttgtttt
     3961 cttctaaaga agcttgatca ttgaatgaaa gcatagctgt caatccttgg gaaatcgttc
     4021 ttgaaggaac aactgctact gggatatctg ctacttctgc tgcttgatca gctgccatga
     4081 agatgttctt attgtttggc aagacaatca ctttatctgc gtttacttct tgaatcgctt
     4141 taacaatgtc ttccgtactt ggattcattg tttgtccacc actgataaca taattagcac
     4201 ctaaactttc aaacagctct tggacgcctt taccagcagc gatcgctacg ataccatacg
     4261 ggatacgtgg tatttctttt ttcgcaaact cgttcacttc ttcatcgtgt tccaaaatcg
     4321 tctcatgctg caagcgcatg ttgtcgactt tgattttgac taatgaaccg aatttttgtc
     4381 cataattcaa gacttctcct gggttttctg tatgcacgtg gactttgatg atctcgtcat
     4441 cattgaccac aagtaaagaa tctcctatac catctaaata gttacggaat tcttcgtaat
     4501 cgaatgtact atcgactgta ggtccttctc cgatttttac catgatttct gtacagtaac
     4561 caaaatggat atcttctgtc gccatttgac cttggacact gcgatgatgt tctgcattga
     4621 ccatatcatc catttctgct ggagaaggtt catatgtttc atccgcttgg aattctccat
     4681 tcaatgcact taaaaagccc tcataaacaa acagcagtcc ttgccctcca ctgtctacga
     4741 caccgacttc ttttaggact ggaagcaagt ctggagtttt agctaaagca cgctttgctc
     4801 cttttacaac agctgtcatt acttcaacga catcatcagt actttttgct ttcttttctc
     4861 cagctttcgc tgcttctcta gctactgtca aaatcgttcc ttctactggt ttcattactg
     4921 ctttatacgc agtttcaact ccatgagtga aggctgcagc aagatcagaa gcatttaatg
     4981 agacaacatc tggaatttgc ttagaaaatc cacgaaacaa ttgagacaaa ataacgccgg
     5041 aatttccgcg cgcacccatc agcaatcctt ttgaaagcat tgctgctaat tctcctactt
     5101 tttctgaggt tgaatctact actgccttag ctccactagt catagataga ttcatatttg
     5161 ttcccgtatc gccatctggc acagggaata catttaatga attgacgtat tctgcattta
     5221 cttgcagacg attcgcacct gcctggacca tttcctggaa ctggcttgca cttatttctg
     5281 ttacactcac ctagataatc ctccttcaga tcgactgctg agttaccagc cagtctcctg
     5341 cctagtctgg cagtacacga acaccttgta caaagacatt aactgaattg gcagttacgc
     5401 ctaataatgc ctctagattg tatttcactt tttcttggac gttgcgggaa acttcagaga
     5461 ttttcgtacc atagctgata atcgtataaa catctacagc aataccatta tcttcttggc
     5521 ggacaactac tcctcttgag taattttctt tacgcaaaat ctcagtaata ttatctttaa
     5581 tttgattttt gctggccatt cctacaacac cgaacacatc tgtcgcggcg ccaccaacaa
     5641 ctgttgcaat tacatcattg gtaatttcaa tggttccagc tgacgtttga atttttacag
     5701 ccatgaatga gcctccttaa cgggtaaaaa actcccattt ttcttctatc atattttatc
     5761 atagcgcaac ttgaaataaa aggcagttac ccaaagtact caaaaacctt gcaaaatttc
     5821 aggtataacc ttgtttaatt tacaattatt cttctcaatt gtcaattgta tctatgaaaa
     5881 tgtacttgca aattcccatt ctatatgata tgttagtctg gtatgagctg aattattcta
     5941 ttgctccaaa tcaaggcaaa ggaggaaatt accatggcaa aagtttgtta ctttacaggt
     6001 cgcaagacaa aaagtggaaa caaccgttca cacgctatga actcaacaaa acgtactgtt
     6061 aaacctaact tacaaaaagt tcgcgttatg gtcgacggaa aacctaaaaa agtttgggtg
     6121 tcaactcgtg ctttgaaatc aggaaaagtt gaacgcgttt aattgaatga tacttggacc
     6181 gttcagatag tgaacggttt tttatttaat aaaaattttt aagggtggga cttttgattc
     6241 ctaccctttt tttgcgttta tttttgttat acgaacaaat tatgaagtta tttattctaa
     6301 attttatttt tttgtgaaaa ctagactctt tttaagcaga gaaaaacaag gatatgataa
     6361 actgaataca acttcataga aatttggtgg aaaataaata tgaaaaaaaa tttaaagtca
     6421 ttctttagag aaaacggacg ctacgcagca gctagtttcc tgattccgtt tttgatttta
     6481 gcacttgttt atctaagtat cggaatctat cctggaagca gcagaagtat tttggcaagt
     6541 gatgcctttt cacagttttc taattttcat gccagcttcc gaaatgttct tcttggaaag
     6601 caaagtattt tttatacatg gaatgcttct ctaggattga attatttatc tttaatttct
     6661 tactatctag gcgggatctt cacgccactg gtgttctttt ttccaaacca gatgatgcca
     6721 gatgcactgt atgttctgac tttgcttaag gtcggctgtg ccggattggc tttttggttt
     6781 cttgcacgta cgttcaagat tcctcgctgg gggcatgttg cacttagcgt cagttacgca
     6841 ttgatctcat ttatcactgc acattcggag atcatcatgt ggctggatgc atttgtttat
     6901 cttccattag tgattttggg gattcatcgt gttatggatc ttaaacggcc gaaactttta
     6961 tttatcagtt atttactctt gtttttatcc agtttttata tgggttttat gattggcgta
     7021 ttttctttct tatattttat cgcccgtcta ttgacaaact ggtcagaata taaaaaaacg
     7081 attgttcctt atggtatcac ttctttactg gcaggcggcg cttcaatgat cattgtattg
     7141 ccagcagtgc tggatcttcg gtcaaatggc gaggcactga gtcagatcac tacgttgaaa
     7201 acagaagcga cttcttatct ggatctaatc atgaaaaata tgattggtgt ttatgacacg
     7261 acaaaatacg gatcgattcc gttcgtttac gcaggtctgc taccattagt cctctgtctg
     7321 ttttattttg ccagtcggaa agtaccgttg aaaaataaat ttttgtacgg cagtcttttc
     7381 gccatactta tcgcaagttt ttatcttgtt ccattgaatt tgttctggca tggtatgcat
     7441 gcaccgaata tgtttttatt ccgttatgcc tatctgttct ctttcttagt gctcctgctt
     7501 gcaggaaaag gatgggaaaa gatagaacaa gacgatcgtg ggctgttgtt aggaatcatt
     7561 ttggtcgtag ctgctctctt tgcggtggca tggggcatca aacctgaagg aagctacacc
     7621 tatgtgacga tgacttcctt tgtattgaca gtggttttct tagggattta tgcttttgct
     7681 gtgggatttt atcagcataa aaaactttca ttcaatcatc tgagtatatt gttattattg
     7741 atcatgtctg ctgaagcact ggtcaatacg aacagtatga tccatggcat tcttgatgat
     7801 tggaattatg cttcccgcag tttatatact gatccttatc ctgacttgaa agctcttgta
     7861 aatgaaacaa aaaaagattc cgactcgttt taccgcttgg aaaatctgga tccagtatct
     7921 gcaaacgatt cgatcaacta tggttacagt ggtatcagtt tgttttcttc tataagaaat
     7981 cgtaattctt cctcttacct ggataaatta ggtttccgtt caagagggac aaatttgaat
     8041 atccgttata acaataatac tttgctgatg gatgggttta caggaatcaa atataacatt
     8101 tctaaaaacg aaagcacgtt cagtaaatat ggcttccgcc aagctggtca aagcggcgac
     8161 tatacactgt atcagaatgt caatgcattg ccactagcat ttactgcacc gttgtccgtt
     8221 aatgatgttg agcagccagt gacagataat ttgaccagtc agacaaactt gatcaataca
     8281 ttgtctggtc taaatgaaca gtattttaca ttctatacac ctactttgaa gaatcagttg
     8341 aatacgacga tcacaaagac aacgactggc gtcacttact cagaaacagc agataaccag
     8401 ccaaaagagc tcacttggga agtccatgtg cctgcaaata cacaagctta cttaagtttg
     8461 ttcccaacca attttgcgca attagaaagt tctagtgcga cgatcactgt caatggtaca
     8521 tcgcgaaaaa cgcaaatcaa catttctggt caatactatg atcttggcta ttatcctcaa
     8581 gagacaacag tgacatttac tgcaagtttt tatgggacga aagaaataag tttcatggag
     8641 cctaaagtcg ttggtctaga tgttgtagcc tatcaagccg caatgaatca gatcaaagaa
     8701 aaaggtgtcg aaatggaagc cactggcaga aaagcttctg ggactgtaaa taccgctgaa
     8761 gattccatgt tggtgacaac tattccttat gacaaaggat ggaaagctaa aattgatggg
     8821 aaaccagtca aggtcagcaa tttcaaagac gcattcttga tgataaaagt tccagcaggt
     8881 aaacatactg tcactttcag ttacttgcct gaaggattta cgataggtgc aatccttttt
     8941 gtcctatgta ttgcattgtt tattctttat gtctggtacc ttgaccgcaa tcgccccttt
     9001 tatggcacga ttgatccagc aatagaagaa caaccacgaa acacacacag aagaagacgt
     9061 agaaaataaa gaaacgatgg aacctgttca gactgtccgc accaagggca atcaaaacaa
     9121 cgttccatcg ttttttggtt acagaaaaaa taggtaacaa aaagatgatt cacttatcaa
     9181 cgcctcgtcc acaagtcacg atattcatca aagatgagaa gctgtttttt gaaattgctc
     9241 tttgtgactc agagttaaat gacaggctca cgacctcttt cttaaacggt gttccagaag
     9301 tagcttcttc gaaaataagc cgaaattttg caaaattttg agaagtaatt ttcgtaaata
     9361 ccttcttatt tcttgaagct gaccacttct atcataacct cttattcata tttcacttca
     9421 tacaaataaa gtccttcagg gtgtgctgta ggtcctgctg cattacgatc ttttttcgca
     9481 ataatatctg ggatactatc ttcaggcaat cggccattgc caattttcaa aagcgttcct
     9541 actaagatcc gtatcatctt atacaagaag ccatttcccc taaatgtaaa caacaattcg
     9601 tcttttgttt catttacctc catcttagcc tcgtagatcg tacgtatttt atcctctatc
     9661 gagcttcctg atgcacaaaa agaggtgaaa tcgtgagtcc ctaataaatc aggaagcgcc
     9721 cgctgaattt tttccaaatc aagtgggtaa ggaaaataac ttgcataatg gcgtcgaaaa
     9781 ggactgcgtg gtttccctat atccactcga aattgatacg tcttttcttt gaccaaatat
     9841 cttgcgtgga agtcttctga aacaatttct acttgtctta cagcaatatc ctctggtgac
     9901 tgggtatcta gcgcgaaacg cattcgttct aacggacgct cctctggata atcaaaatgg
     9961 attacttgcc cgatcgcatg tactccggca tctgtccttc ccgaaccaaa gaccgtgatt
    10021 tcttttccat ttgccatttt ttgtaatgtt ttttctactt cttcttgaac agtacgccca
    10081 tttggctgtt tttggaatcc attaaaattt gttccgtcgt acgcaatgat tgctttgtaa
    10141 cgtaccatgt ttttctctcc tttgttccta aaaggttatc tggaataatg
//
LOCUS       DADXTH010000088         9934 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.088, whole genome shotgun sequence.
ACCESSION   DADXTH010000088 DADXTH010000000
VERSION     DADXTH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.088"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            573..1727
                     /locus_tag="IWI97_002361"
     CDS             573..1727
                     /locus_tag="IWI97_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBC2463840.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1721..2143
                     /locus_tag="IWI97_002362"
     CDS             1721..2143
                     /locus_tag="IWI97_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463841.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2445..2825
                     /locus_tag="IWI97_002363"
     CDS             2445..2825
                     /locus_tag="IWI97_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBC2463842.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            2807..3721
                     /locus_tag="IWI97_002364"
     CDS             2807..3721
                     /locus_tag="IWI97_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBC2463843.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3961..4650
                     /locus_tag="IWI97_002365"
     CDS             3961..4650
                     /locus_tag="IWI97_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463844.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4770..4856)
                     /locus_tag="IWI97_002366"
     CDS             complement(4770..4856)
                     /locus_tag="IWI97_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBC2463845.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            5502..6434
                     /locus_tag="IWI97_002367"
     CDS             5502..6434
                     /locus_tag="IWI97_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBC2463846.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            6451..6921
                     /locus_tag="IWI97_002368"
     CDS             6451..6921
                     /locus_tag="IWI97_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBC2463847.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            7005..7309
                     /locus_tag="IWI97_002369"
                     /pseudo
     CDS             7005..7309
                     /locus_tag="IWI97_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7584..8765
                     /locus_tag="IWI97_002370"
     CDS             7584..8765
                     /locus_tag="IWI97_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBC2463848.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            8855..9124
                     /locus_tag="IWI97_002371"
     CDS             8855..9124
                     /locus_tag="IWI97_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBC2463849.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYK
                     KYPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
BASE COUNT         3689 a         1575 c         1637 g         3033 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaacaaacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcacgcgctt
      241 atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt aaaaaaatga
      301 cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag ctttagaaag
      361 ttgcttttag ctagaccaat taaaaatcac aaagtgagcc tctgtatcgg ttgatacaag
      421 ggctacttgg catagtcgac attatgttaa ctaacgctat aaataacgat tatgttccca
      481 aaacaatatt ttatgaacta taacaaaaat ttgttatcgc atattgtata ttttaattat
      541 aggtgtatac cacttaaatt taacacaaag gcgtgattat tttgaaaccc aaaaagaaaa
      601 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
      661 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
      721 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
      781 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
      841 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
      901 atatttatac cgtcaaaaaa aatacaaatg gagaatattc aggaagtatt tcacaatttt
      961 tagcaaaaga acttcaaaat ctagtagata gtaatataac agatacaacc ttattttttc
     1021 aagataatag catttattat tccgataatg gacaaattaa actactttgg aaatcacctt
     1081 taagtacaga aaaaaatggt gtttcttcct ttacagaaaa aaatgatctt atatccgtaa
     1141 atataaaaaa taaaattaaa aatttctcta aaaaacaaaa ttttcctgcg attaaacgtg
     1201 taaaaagtgt ctcctccgga gataacttta ttatgattaa ttggcgtata gcagaaaccc
     1261 aaacaagttt tccgtggtgt gctgcttacg tagaagctgc tatcttatgt aacaaaacag
     1321 atgtacatgc tacttcagct agagccatta tgcattatac ctatcctaat ttaaacaaca
     1381 gtcaaattga atcaaaaagt cttaatcaag atcaaataat aaaatatgca aattactgtg
     1441 gtgtttatcc taaaaaagta gcacgaatac taaacctttc agaggtccaa acacaactaa
     1501 gaaaaggaaa tgccatttat atgggaactg ctggttatgg aaattatagt aacagccgtc
     1561 atgcacttgc attatttggg tgggtcaatc aaggtggtta tcaaacttac tatgtgtgga
     1621 atccttggta taactaccca actgtaataa gtgcaaatgc aggaccagtt acaattcctg
     1681 tacctggagg tggttttact tgggacaata ccattacaaa ttggtaaata aacccattgc
     1741 tttagttatg ggtctagtgt tagttctttt cttaactagt tgttcaaaaa aacaagaaat
     1801 acaaaagact gatctgattc cttttctagg aacttggcaa actaaagatc attcagaacg
     1861 tctagtaatc actaatttaa atacttcaaa aaatatagaa caatatacca tcttagtaaa
     1921 agagaacaat cataaaattc aaatcattga taaaaattta aatataaatg aacaagtttt
     1981 catttacaaa gcaacaaata atttactaac actgcgctct aataaaaaac aattagatta
     2041 cacttttagt ttggattctc caaaaaagct cgtatttatt ttgacaacga atgatgggac
     2101 agaagggaca gctaaaccaa ttgaattttt taaagctgat tgatgaatag ttgtttgata
     2161 ttacaaaaca ataatttcac cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc
     2221 atttgctagc cttttttgta ttgtggagcg tgggttttga gggggattca agggggagct
     2281 tgcgcccctt tgaaaagcgg ttatttgcac caaatacgta ggtatttggc tagcaaatat
     2341 agagcttgcc aaaccctttt agtgagcttt actcattgta cctttttagg tacaatggta
     2401 ttgcgaacga tacgtccgta ggacgtactg aaagggtggg acttatgagc gaacaaaatc
     2461 aaaatttggc tagcgatcat tctaaaaaat atacctatcg atccgagcca aaacaaatca
     2521 gttttcgagt gagcgaatct gaatttgcaa agctgaagca gtcagctgaa gctttgcaaa
     2581 tgagtgtgcc ggctttcgta aaagccaagg cacaaaacgc gcgtctggtg acaccgaaag
     2641 tggcgccaga cattgcccag gcaatcgcgc gcgatttagc caaagctgga gggaatatca
     2701 atcaaattgc taagtggtgt aatacacacc aacacgacgt tgctcctgga gacgcccaac
     2761 gcttatccga aaacctaaaa atcatgcaaa aggagctaca aaagatatgg caacagttaa
     2821 agtaagccga accacttctt gtagtcgtgc aattaattac gctgaaccac gagcaaccat
     2881 taaaacagga ataaactgtg acgtcgatta tgccaaaagt gaaatgaaac aaattcgcat
     2941 gctgtatgga aaagacgacc gtgtacaggc acacttattg attcaatctt ttcgaccagg
     3001 agaaattacc gctgaaaaag ctaatcaact aggggcagag tatgcaaaaa aaatagcccc
     3061 cgatcaccaa atagctattt acacacatac agacaaagac catattcata atcacattgt
     3121 cattaactcc gttaatctgg agactggaaa aaaatttcaa gctcatggtc aagcatttct
     3181 aaacaaatgt tatgacctaa atgacgagat ttgtcttgca cataacttac caatcactga
     3241 gcgaggaaag aaaccagaaa aacgaaccat gtctgaaata aaattaaaag aaaaaaatga
     3301 acctgtttgg aaagacgaaa ttcggattgc gatcgatcaa acaatgaaaa acaaaaaaac
     3361 cagaacctat gaccaatttt gtaattccct caaaacattg gggattcatt gcttcaatcg
     3421 cgggaaaaat ttcacttacg aattgatcca caaaaagaaa aaagtacgat ccaacagact
     3481 aggaaaggac tacgaaaagg agacgatttt acatgagctg gacagacgag aacgaaccta
     3541 taaccaacag cgaaccaacg agctacgaag agctcgagct agcattcgcg aaactgcaga
     3601 agaagtacga agagaacaac gaactcttac gcctgaacaa ccggaaatta gaccggctcg
     3661 aagaaaccat gaacaacacg ttgaaagagg aacagaccac caacaagaat tatcgctcta
     3721 aaacgttaaa agacatagaa aacgaaataa agaaacaacg tgcttttctc gttccttata
     3781 cgcaagaaag gatacaaact acgattgatt tacaagttaa aaaactccat caagagctat
     3841 tagaccagcg aaataaggaa cagaaaaagg tgcttattca acgatcattg gattttcttg
     3901 cccccttatg ttgcttagtc ctgacactgt tcgcattttg gttagtatgg ggaggctaaa
     3961 atggcatttt atttacccta tctactcatt tttgttagta tttcaggaag tatttggctc
     4021 atctataaaa tttttcaaac aagatattca ttaaaaggct ctaaaattcg atttaagcga
     4081 tttttctttc ttggctgtat attttccttg ataatcgttt caagcggctt attcggcgtt
     4141 ttagagggaa ataagcgcgt ttctagaagt attttattag gaaatgtcac tcaaaagtat
     4201 gaatcagcgc gtgataaaaa gaaaaaagag caagtattag cacaaaaaat agaaaaattt
     4261 accgcttgtt atgaggacat gaatgatatt tttgtgaagc aagaaaagcg tttgacggat
     4321 aaaaatatgg aaacactcac tcgactttat cggaatttgc cagaagaaca gcaaaaagag
     4381 taccaagaaa aatatgaaca agtaaaaaaa gacgtgcaat atgtgaaaga tacgaaagtg
     4441 gaagaagctt gtagtgattt atttggagat acaattcctt tttcaactag cgaacaagaa
     4501 cgaaaagaac gacaacaatc agtcacatat gaacgctatg aaatcttgct tcaacaagca
     4561 attaacatac aaaatctgac taaaaaagaa acagctttaa attatttaaa aagcgtcaag
     4621 gaatggttag atcagcaaca gcaaaattga atttgattga aattcatgtt aaactaaaca
     4681 tgcaaatgtt caaacattag atacacgaaa gccccagaga ggtcggattc tctggggctt
     4741 tttttaggtt gatgtcgcct ttactgattt tacttcttgt ttcggttatt caaccaataa
     4801 ctgaatacag agatacaaac tcctacaaga agggggcaaa taaacagttc aaacatcaga
     4861 tacacctcct tccagtcgca agactgtcgg ataggcgaca agttattata ccctacaaaa
     4921 ttaaatctgt gtggcaattt taagtatctc ttatttccgg gttgttaggg cttgccctga
     4981 ccgcctgtag ggcgctttaa atgccagata aaggttttta tactaagact gtcttaaaac
     5041 ggcttagaca agctcttaga gcctcaaccc tcctttttga acaatggttt tagcaaagga
     5101 gctggaggcg actgcgcagg tataatgtga gagcgtagcg atcacacctt tttggcgacc
     5161 gtagggagcg aggttttgaa ttagcagttt agaaaagttc gcgaacctgt ttaaagaaaa
     5221 aataattgtt tttttaggtg ggaaattttg tctccctctt ttataatctt tttttaaacc
     5281 tctttttaaa cctctttacc gtcggctgtg tccttactcc cccaagggat acaaaatttt
     5341 ataggcatga aattatctgt ttataggcat gaaattatct gtttataggc atgaaattat
     5401 ctgtttatag gcatgaaatt atctgtttta ggtattgttt ttagacataa tatgtcctaa
     5461 aatgatgtca ataaaatcat ttagactgga gtaacaacta aatggcaaat gaaatcgtaa
     5521 agtatgaacc agaattaaat accattcctt taagaaaatt taccccaata gaaatgaact
     5581 tgtttttttc tattgtctct agaatgcgcg ataaaggtaa tcaaaaaata cgcttttatt
     5641 tcagccaact taaagattta agtgcgtata agcagactgc aggagaagtc tttataaaag
     5701 atttggcaag aacatacgat catttgatgg atttacgttt cggaagaaaa agcaatggtg
     5761 gattaacaat agaacgattt acaatgttta ctgattttga aattcatgga gaagtcgata
     5821 acccttatgt agatataaaa gtccacgaaa aagctttgcc tttacttaat aatctagaaa
     5881 gctgggtacg tttctcttta acagaatttc gagatttaaa tagcagttat gctaaaacaa
     5941 tgtttagatt gctaaaacaa taccgaactc aaggatgggc tgaattttct aaggaagatt
     6001 ttttagagct attagatatt cctaaaagct atagacaaac aaatataaat cagtttgtac
     6061 tcaaaccaat aaaagaagaa ctaactccac tatttaaagg attaactatt cgtaaaaaat
     6121 atggtaaagg tcgaggtaaa cctgttattg gttatcgttt tacttggaaa gctgaaatca
     6181 accatgctga cgatttttca aaagggaagc aagaagattt acgtataaaa ttatttaata
     6241 ttgaacacaa tggagaatta acacaaaaag aaaaatggcg tgctaaagat cgaatactaa
     6301 atcttccgtt aggtactcat gaagctgatt ttaaaaaaca acaacaaacc gaaaaagaag
     6361 aagcggaaaa acaatcaatc tcaaatgagc ttaaacaaga tttattagaa aacttacaaa
     6421 acttattcga ttgaagcata aggagtattt atgatagata aaacgatcaa agaactcgcg
     6481 gaagaatttc atgtttcaaa acaagcaatt aggaaaaggc tatcagctga ttttagagaa
     6541 aactatgtgc aaactgttac taggaacgga gtgaaaacct tagttgtcag ctacaaagga
     6601 tatttgcttt taaaacaaca ttttttaggt agtaacacca ctggtaacca agtagaaacc
     6661 gttgctagta acgatgaaaa ctcgattatt gtttttttac atgaacagct ccaagaaaaa
     6721 gatactcaaa taaaacaaat gcaaaagtta ctcgaccagc aacaacaact caccttgcag
     6781 gcaaatcaac agatcgaaaa actgcaagaa cagttacaat tagcttatac ggaagaacct
     6841 gaagaagagg aattaacttc tgtcaataaa gaaaacaaca atgcaataac aacagaaaaa
     6901 aagtggtgga aattttggta acaattacca actttccata aagaagaaat caatttctat
     6961 agactctatc tttctaagaa taaaaaatat gtttaggtga tcaaatgact ataagtaaag
     7021 taatcaaaca aaaaagaata gaaaaacaac taactcaaga agatttagct gaaatgcttt
     7081 tagtatctaa aaaaaccatt tctaattggg aaaatggtcg aacaatacca gatacagaaa
     7141 atttaattca gtttgctaag ttatttgatt tatcactaga caatattttg ttaggagaag
     7201 aaaaaattat ggtaaaaaat catacaaaaa cactaatact attatttcat tttgtaattt
     7261 ttttaagttt agttttcacc ttaattcagt ccggattctt ttattttagc cattcaattg
     7321 ccacttggtt attaatattt tttctaagtt tactattaat aattaatact ttaattatta
     7381 tctttaataa acatagaaac ttatttcttc acagcatatt tccaattgtt ttattaattt
     7441 ttctatactt tatataaaaa gatagaacta tttagtaaga taaaaaagta gctaattgta
     7501 attttcgtct aaaactcaaa actagtaaaa ataactttat tgtgttattt ttatttataa
     7561 attatgaaaa aggacctttc gaaatgaaaa ataatagaat ctacaaaaat cagttgaatt
     7621 atgggctttt attgccagtt tttctgctat gtatcattgg attactttct ttatatgttg
     7681 ctttatatca cgactcttac actaattcaa aaatcacaaa agaattaatt aaacaagtat
     7741 tgtggtatag tattgggttc acttcagcaa taactattta tttttttaat cctaaaattt
     7801 tgtggaaatt aactcctata ttttacggat tatgcatcat aatgttatta cttctcttga
     7861 aattctataa tccacaatta gcagtcttaa ctggagcaaa aaattggttt tatattgctg
     7921 gtttttcgtt tcaacctact gagttagtca aaataggata cacccttatg ctagctaaaa
     7981 taaccgtcaa ttatcagcaa aaatctatta ctagtaatac gaaagatgat tggatttatt
     8041 tacttaaaac ttttattgct actcttccaa taggaatatt attatttttt cagaacgatc
     8101 taggaacaat gttagtcttc attgttattt atatttttat tttgtttacg tctaacataa
     8161 atataaaaat aattgcccca ataatcgcca ttggtgttgt tttatcctta atatttttat
     8221 atttggttat atcagataca ggaagagatt tcttattaaa aataggattt cacgactatc
     8281 agtttaaaag aatagactca tggttgaatc catttttaga tccaaatgga tctagctatc
     8341 agttagcaaa tagtttaatt gctattggct ctggcggact tttaggtact ggttttaatg
     8401 tatcaaacat tcacgttcct gttagagaat ccgatatgat attttctgta attggagaaa
     8461 attttggctt tataggaagt tgtcttgtaa tattcctata cttttattta atttatcaaa
     8521 tgtataaaac atgttttaaa agcaataact tatttttcac ctatatcggt ataggcattg
     8581 ttgcaatgat tttttttcat gtttttgaaa atataggtgc aagtatcgga ttactacctc
     8641 taactggtat tccactcccc tatattagtc aaggtggttc tgccattctt agtaattttt
     8701 tggggttggg atttatgttg tgttgtagta gatatactaa cgaaaaaaaa ggaacacaaa
     8761 aatgaattca aaataacact ttatgaatag aatgtctatt tcaataaaaa ttatgatacc
     8821 ttaaagttaa tcattaaaaa aggagtgtca taatttgaaa aaaacaaaat tattggtagc
     8881 tagtttgtgt ttgtttagca gtttattagc tttcacccct tctgtttcat tttctcaaaa
     8941 tggtggagtg gttgaagctg ctgctcaaag aggatatatc tacaaaaaat atccaaaagg
     9001 tgctaaagtg ccaaataaag ttaaaatgtt agtaaatatt agaggtaaac aaactatgcg
     9061 tacttgttac cttatgagtt ggaatgctag tagtagaaca gccaaatatt attactacat
     9121 ttagtaaaaa caaaacattc tttctataca ataaaatagc gtatagagag gatgttttta
     9181 atttgaaaag gagtggttat ctttatgaaa aatagatcat ttgctatatt agtattatcc
     9241 gtaattttat tagatatatt gttaataaca ttagctttaa aaactttaaa tttagtaaca
     9301 agttttgtca ttataccagt aattatagga atgtctattt atttcatcaa aaaaaataaa
     9361 caattttttt gaatgaaatt acgggcgaaa ataatcactc aaaattatta ataaaaacga
     9421 atcaactact ttttctgaag aaacatacaa ccgtaaacaa caattgttgt ttacggttgt
     9481 aaattatcaa actatatggt gacttgttgc ttaaaatagg ttgaaatttc gtttattttt
     9541 tttgagaaat tttacccacg tactacccat gtacctatcc acgtattacc catgtacccc
     9601 cccatgacaa atgggcggag gggcttgcgg cgcttggtat taaaggcatt atccgcctcc
     9661 caggtttctg accattttct ctcataagtg gggtttttac cgcgttgaga gaaaatggtc
     9721 agaaacctaa acgatcggca aagaaaacat accaagcgcc acaagcaaaa aaagttagag
     9781 taacttccag aaaagaaaaa tttttagaac aacttttgaa aatttgattt tgatttatat
     9841 ccgttcaatt taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg
     9901 tagcgttaaa aaaagtggag gtataaccta tgtt
//
LOCUS       DADXTH010000089         9873 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.089, whole genome shotgun sequence.
ACCESSION   DADXTH010000089 DADXTH010000000
VERSION     DADXTH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.089"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(96..2216)
                     /locus_tag="IWI97_002372"
     CDS             complement(96..2216)
                     /locus_tag="IWI97_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBC2463850.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2446..3624
                     /locus_tag="IWI97_002373"
     CDS             2446..3624
                     /locus_tag="IWI97_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBC2463851.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3640..4398
                     /gene="aroD"
                     /locus_tag="IWI97_002374"
     CDS             3640..4398
                     /gene="aroD"
                     /locus_tag="IWI97_002374"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBC2463852.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4421..4906
                     /gene="ybaK"
                     /locus_tag="IWI97_002375"
     CDS             4421..4906
                     /gene="ybaK"
                     /locus_tag="IWI97_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBC2463853.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4977..6230)
                     /locus_tag="IWI97_002376"
     CDS             complement(4977..6230)
                     /locus_tag="IWI97_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2463854.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6347..7696
                     /gene="gdhA"
                     /locus_tag="IWI97_002377"
     CDS             6347..7696
                     /gene="gdhA"
                     /locus_tag="IWI97_002377"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBC2463855.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7808..9154
                     /locus_tag="IWI97_002378"
     CDS             7808..9154
                     /locus_tag="IWI97_002378"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBC2463856.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9462..9848)
                     /locus_tag="IWI97_002379"
     CDS             complement(9462..9848)
                     /locus_tag="IWI97_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBC2463857.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3148 a         1793 c         2023 g         2909 t
ORIGIN      
        1 ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg agcagcgaca
       61 ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact tgtggtcgtt
      121 ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg tccgttgtgt
      181 tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt taatttctct
      241 aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact attcgtatag
      301 aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt atcaactttt
      361 tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat agcttcacca
      421 gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac aaaatcacca
      481 tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc taactgaata
      541 taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc aacttgtccg
      601 ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat ataagggaca
      661 cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc aactaattca
      721 gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag gacgatgact
      781 gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac ggcggtatct
      841 aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt ggctaacggg
      901 aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac agcaatgaat
      961 tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg gaaaaatggt
     1021 ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata agaagaatca
     1081 aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa tgtaccagcg
     1141 ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc aggtgcagct
     1201 tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa gccaaacaat
     1261 gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg atggaaaaag
     1321 ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg tgtaacttca
     1381 tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg gaagctttct
     1441 aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc atttggtgct
     1501 gcataatgtt cttttacata attttctact tctttcatat cattgggact tgcttctgcc
     1561 cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc attcaagcca
     1621 agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg acgatcagct
     1681 tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat cgcaaaagcc
     1741 attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag cagaacagtc
     1801 aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg aatggcactt
     1861 tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc
     1921 gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag aaaataaatg
     1981 accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat tccaagtaat
     2041 aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc actttcgatt
     2101 ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa aacgactagt
     2161 aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca tttcaagata
     2221 atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta cacagctcac
     2281 ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca gaagtttatg
     2341 ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga caagtcctat
     2401 gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa agtaaaagta
     2461 aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa agaagacctt
     2521 gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca aaaccaattt
     2581 atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt gagccagcag
     2641 aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga
     2701 actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg agaaggagac
     2761 gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc ttggtataac
     2821 gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat ctacccggaa
     2881 atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga gagccagttc
     2941 atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc
     3001 gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga agtacgggga
     3061 tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag ttataccggc
     3121 gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt cgatctagca
     3181 aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga tgtcgatcag
     3241 caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag aaaacaattt
     3301 ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa aaaagtcttt
     3361 cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt agcagaagac
     3421 gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg
     3481 attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt atatcgtctg
     3541 ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa agtgtttgtt
     3601 tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt aaagggattg
     3661 aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt ccgagaggaa
     3721 gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga atggcgagtc
     3781 gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt tcttatccat
     3841 gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga aggcggagaa
     3901 caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca cacaggatta
     3961 gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag actgatagag
     4021 gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa agaaacacca
     4081 gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc cgatatcgga
     4141 aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga gttgactaga
     4201 cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa tcttggtaaa
     4261 atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc cttaagcagc
     4321 gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat tattcaatta
     4381 ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga aaaaaatagt
     4441 aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag agcacgaata
     4501 tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc cagaaagcca
     4561 atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc ttgttgctgt
     4621 gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag ggaataaaaa
     4681 agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac gtggtggctg
     4741 ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg cagaaagcta
     4801 tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag cacctcaaga
     4861 tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca tacgtataaa
     4921 agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt tatacattat
     4981 gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc tgtgatatta
     5041 aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag accatcaatg
     5101 attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc gccagttaca
     5161 aatgatttct caataggaaa agttttgttg attgatagct taaactcttt gcatagttga
     5221 acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc catttcaagc
     5281 cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc attgaccaag
     5341 ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg tttggtttgt
     5401 gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa ttctttattg
     5461 acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa
     5521 tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa agtgatgtct
     5581 tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga tatcaagggg
     5641 gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat cttgtaccca
     5701 tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat aatggcagga
     5761 gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat
     5821 ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg attgagcaat
     5881 gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg atcctcttct
     5941 tttaaatgta cgccttgtga ttcaaagaag ttcatgatat cgaaattatt ccattgcgca
     6001 aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc atcaacagga
     6061 cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt tcctgctttt
     6121 ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc
     6181 atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat tccctcacct
     6241 caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg agccaaaaga
     6301 gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga caaacgcaac
     6361 agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc aagaagaatt
     6421 tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca ctcatcccga
     6481 atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta ttcaatttcg
     6541 agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc gtatccaata
     6601 caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg tcaatctaag
     6661 tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg gtctgccaat
     6721 cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata atgagatcat
     6781 gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat cgattgatgt
     6841 tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg gcgaatataa
     6901 acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt tttggggaag
     6961 taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac atctattgaa
     7021 cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc
     7081 catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat gttcagattc
     7141 tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga
     7201 aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta tttatcatgc
     7261 tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat gtgctactca
     7321 aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca aaatcgtagc
     7381 agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg aagcaggcgt
     7441 gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg ctttagaaat
     7501 gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc agctggatca
     7561 aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa
     7621 aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag caatggcagc
     7681 agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt ttgtttattt
     7741 gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc tataaggagg
     7801 agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg tcaacgaaca
     7861 agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac gggaaggtac
     7921 tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg acaaagaaga
     7981 atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag tattagttgt
     8041 tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct tgaatcacac
     8101 attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct ttgccggaaa
     8161 ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc gcgacttttc
     8221 agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt tccgtgtatt
     8281 caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta tctacgctac
     8341 aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt gggaaacttt
     8401 cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg gtttgttacc
     8461 aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa
     8521 agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag caatgcgcaa
     8581 cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac caggaatgca
     8641 atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag accaaaaagg
     8701 catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac aattcatcca
     8761 agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat
     8821 cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag gaaaagaagt
     8881 ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag acggtgacgt
     8941 gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt atacaatgta
     9001 cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc cattcgacca
     9061 accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac caggctttga
     9121 agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc tagatttaaa
     9181 taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt taggtactca
     9241 aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt cacagtccct
     9301 tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga ctttaagaac
     9361 agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca gtaagcaaaa
     9421 aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag gcctaatttt
     9481 tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat gatccgcttt
     9541 tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt ggcagagtca
     9601 ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc ataagcatct
     9661 acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat atcagtttct
     9721 gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt agactgatag
     9781 tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa ccctaaaatt
     9841 tttttcatga aagatctcct tttttatttt tat
//
LOCUS       DADXTH010000090         9819 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.090, whole genome shotgun sequence.
ACCESSION   DADXTH010000090 DADXTH010000000
VERSION     DADXTH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.090"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            55..507
                     /locus_tag="IWI97_002380"
     CDS             55..507
                     /locus_tag="IWI97_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463858.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            521..1210
                     /locus_tag="IWI97_002381"
     CDS             521..1210
                     /locus_tag="IWI97_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463859.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1335..3686
                     /locus_tag="IWI97_002382"
     CDS             1335..3686
                     /locus_tag="IWI97_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBC2463860.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3766..4161
                     /locus_tag="IWI97_002383"
     CDS             3766..4161
                     /locus_tag="IWI97_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463861.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4486..5103
                     /locus_tag="IWI97_002384"
     CDS             4486..5103
                     /locus_tag="IWI97_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2463862.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5144..5443
                     /locus_tag="IWI97_002385"
     CDS             5144..5443
                     /locus_tag="IWI97_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBC2463863.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5477..5644
                     /locus_tag="IWI97_002386"
     CDS             5477..5644
                     /locus_tag="IWI97_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2463864.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5689..6294)
                     /locus_tag="IWI97_002387"
     CDS             complement(5689..6294)
                     /locus_tag="IWI97_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2463865.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6419..9406
                     /locus_tag="IWI97_002388"
     CDS             6419..9406
                     /locus_tag="IWI97_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBC2463866.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            9555..9764
                     /locus_tag="IWI97_002389"
                     /pseudo
     CDS             9555..9764
                     /locus_tag="IWI97_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3359 a         1479 c         1879 g         3102 t
ORIGIN      
        1 ttatcttatc gtagatatat attataaatt taataagaaa gcgaggtgag aatcgtgaaa
       61 aaaacgatac taagtatttt ttcagtattt acaatattaa ttgggggcta ttttcttttg
      121 aatttagatt ctgttaattc aaacgctcaa ataaaagatc aaagagtagc tgaacataat
      181 aataaaaaaa tgagtttgac caaaaatatc tattctatgt cagatgaaga attgtataaa
      241 gaatttggtg atttatccgg ggaacagtta gaagaaagta ttccaggaat tagtgactca
      301 gataaaattg ttgtcttgtc tgctggtggg caattaatgg gagatggcaa atccgaatta
      361 gttagttata aacaaaatca cagacagttt attgatgtta atactgattc caacgctatt
      421 tcgattaagg atctaaaaga gcttttaaat agtaaaagaa atgaattaaa gcagataaaa
      481 gaaactaatt cagtggaatt agagtaaagg aagattcatt atgaaaaaga tttttggtgt
      541 ttctgttggg ctacttatga tgttaacatt tggaaatgca gtatatgctg ctgagaaaga
      601 agttatttca gatccagagc agtacgcctc acgatacttt gctgattttg aagaagaaga
      661 taaagtactt gcattgccag aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat
      721 ggataatcca gatataattc aaggttcata taattcagaa actgatcctg cttcagtttc
      781 agtgacagaa gcaaaaaaaa ttgttgctga aaaattcaaa aatgatattg aaactactcc
      841 aattatgcct cgaggagcat ttccacccga tgttggtact agcggacacc ttcttgtact
      901 agcaggagga ggaagctata aatcacagcc atttagtggt gcgggatggc gttttggagg
      961 tatgggtgta aaaccagctg atggtacagg caacttttta cgttggaaat cctacattga
     1021 cgatggaaga gtaggaaatg taaatcaagc caacgcaact aaatctggtt ctattcaagg
     1081 aatggcattg tatgctcgtg aaggattcaa atactttgca ccaaacgggc ctggttggac
     1141 aatgaatata ggtatttatt acacttataa tcctgttgca ggtacgtatt atgttgttca
     1201 aaatatttaa taaaacagca caaagagatt tctttgtgct gttttattat ttttagttgt
     1261 tgacaaacac taaaatataa aatataatca acacataaac gaacgtatgt tcggcaagaa
     1321 agcaggggat ttttatggat gagatggttt taagtactca gaaatggcta aacaagaaat
     1381 acagtaatgt tacagggttt gataaggttc cagaaaatgg gcgaacaggg tggcctacaa
     1441 tctatggact tattgaagga ctacaagtag aacttggtat tactaatcta gtagctaatt
     1501 ttggacctac cactgaaaaa atgtacgata atcaggttac tcctaaatgg ggcaaaaatc
     1561 tacctaaaaa tattgtattt cttattcaag gtgccttttg gtgtaagggg attaatccag
     1621 gtggcttcga tggggtatat actccatatt tagatactgc tgtaaaagag ttgcagactg
     1681 atgcagggtt taatggaagc gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt
     1741 tatttgatat gagtgctttt gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc
     1801 agcaatggtt aaatgtaaat tatttagaat acacgggaat aatgccttgt gatggtattt
     1861 atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca agctgaacta ggatattctc
     1921 ccagtgaagc aacagggtct tatggtaatg ggacaactag taaaacttat cctgtgtctg
     1981 aaggtaactc gggaaactat gtaagaataa ttcaatatgg attatatgtt aatggttatt
     2041 ttgaagacgg tacattcgat ggtattttca ctaaatatat gggtttagaa gtactggcat
     2101 tccgaaaatt tatggtattg cctgaatata ctgaaatagc agatgttgtt gtaataaaag
     2161 gactattgtc tagcgctgga gatattagaa gatctgcaga tggcgcagat acttctacgc
     2221 aattaactag atctcaaatc caaacattgg ttgataatga tataaaaata gttggtagat
     2281 atcttactgg tactgttgga attggaaaag atgaacgaaa taagtatcta actacagagg
     2341 aattgaataa tattttttct aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg
     2401 cagcattagc ttattttact tatgatcgtg gcttatcaga tggtaagaag gctatagatg
     2461 cagcaaaaaa tctaaatata ccattaacaa cagttattta ttttgctgtt gatctagaca
     2521 tgttagggga agaaattctt gcatatgctg gagaatattt taagggggta tctgctgtaa
     2581 tgtcatatag cgggtatcaa acgggtgttt acggaacaag aaatgtttgt tctcaaataa
     2641 tcaataacgg gtatgcttcg tttgcatttg tttcagatat gtcgactggt tatagtggga
     2701 atttaggttt tcctatgcct agacaatggt cttttgatca atttgttgaa tttaccatag
     2761 gaagtggcaa cggagctgtt ggtatcgatt taatcgctac atcgggtaga gatagcggat
     2821 ttaatgagtt atcaaatgat actaataatg ataattacat tgccaaatac aatcagaagg
     2881 ttataaatca gatggtacgt gcgtatcaat atctatcaca agctggtctt gataatcctt
     2941 ggaaccctca tttgacattt tatagatatg taaattattc tggtttgtca tgggacatca
     3001 tttcttctcc tgtaacagag catgatagaa aaatttatga tgaatatagg aataaattaa
     3061 ctaatagcga gggcctgtat aattatttta tagatcctaa tactggttca ataataggtt
     3121 tgcctcactt aattgtgacg ttacaatcac agatgtttct agccgacaca atcaatgata
     3181 gtgtttctga ctttgcaggt tggttaggag atttaatgac ttgctggggt gaagttaaga
     3241 aattaggaat acaaatggag caaggtgtat ttgcattagt aggtacggct gcaggtggaa
     3301 catttagtct agaagattta taccaagatg ttgacgcggt aaatgtttat accttagcaa
     3361 aaagaaattc tgattcaaca gctttatcgg ctttaaaaac gtactattgt gataagggtg
     3421 ctattgatag atttaatcaa tttgttaaga acagatttgg ttcttcagat aaaatcgctt
     3481 cagtaacaaa tagtttatta gaaaatgacg aaacagttga tatcttaggt gtagggataa
     3541 tttcattttt cattgataaa atagttaata attcacttgg gacagatttt acttatcttg
     3601 attttattgg agatgagggg aaagttgcaa atggattttc tgaaaaaatt attcaatttc
     3661 aagaaaatag aagtaaactt aattaagaat cgtttactat ttaaacagtg tccttttcct
     3721 ttttaaatat atacgataaa ataagtatat gaattgtaaa aggacatgat gattatggaa
     3781 gaaatagaat ggaataatct gactcaaaat aataaaaata attctggatg tggaattatc
     3841 tttatgtttg ttatagtttt gggaatagta gtacttttaa taactgtcaa gatagtagat
     3901 tactttgcga atactcctga agaacaaata cagagaaaac ttcctgagtt tgaaagagta
     3961 ccaccttatg atccaagaga taattcattt caagtaaaaa aattaaaaga gtctccaaat
     4021 gaaatgtggt ttgaagttac agttgaatca agcgacttca aagaaaaaaa gaaagaatac
     4081 aattatattg taaaggcaaa agtatcaaaa agatttattt ttaaccctaa attagaaata
     4141 tatagtgttg taatagaata atagtgttaa aaaaattgta aaagttaaag aagtcaaatt
     4201 ctgatgtgaa aaataaaata taccatggaa gacataaaag aaaaactgga taactagttt
     4261 atttaatggc agaccgttaa ggcctgccat ttttatgtat tttagcggaa taaatataat
     4321 catgcgggag tgtaaactta ctgtttcaaa aagtatactt tttgacatgc cttgattttc
     4381 tttgtttatg tatggcataa taaccacata atacaatttg aaacgtttct gatttaatct
     4441 gattgttttg aaacaatttt agtgaaaaaa gggtagataa aaacgatgag aaaatttttt
     4501 tatatccggg tcagtagtta tgatcaatcg atcgaacgac aactgatttc cgcgaaggaa
     4561 ttaggaatcc ctgaagaata tatatttgta gaaaaagcca gcggcaaaga ttttaaacgc
     4621 cctgaatatc aattaatgaa acggatgttt cgaaaaggag atttactcta tctacaatca
     4681 ttagatagac taggcagaaa taaacaaatg attttagaag aatggcgaga attaactaag
     4741 gaaaagcaga ttgatatttc tgtgttagac atgcctctgt tagatactgc tagttacaag
     4801 caactagatg ggttagagac gcttatctct gacttaattc tgcaattact cagctatatg
     4861 gccgaagacg agcggaaacg aattaatagc cggcaaagag aaggcgtaga agctgcgaaa
     4921 caaaagggtg taaaattcgg cagaaagaaa attgctttac ctcctgaatt tcctgcgatc
     4981 tattctgact ggaaagcagg aaagataact gcagtctatg ctatggatca attgggcatg
     5041 aaacgaaata ccttttatcg acgggtgaaa gaatatgaac ataccttgca ccacttttct
     5101 tgatagatat aacagctatt agacgggaag ggagagtttg aaaatggact taacgaatca
     5161 aagggtcgtt tttactggaa cgctgcagca aatgacacga acccaagcca tcggtttggt
     5221 tcactcttta aacggccatt gtcaatcaag tgttacttca aaaacaaatt tcctggtttg
     5281 tggtcagtat tctaagtcgt tgtttgatga ttctctctat acaaaaaagg aacaaaccgc
     5341 gcgggacttg attgcgttag gtcatgagat taccatttta tcagaagtcg aattttatgc
     5401 gttaattagt caacagttga aagaatggca acggtattta taatcgttag taaaatttac
     5461 aaggaggaaa atttttatga agcgaaaatc ttatgatcgt tggggcattg aaataactag
     5521 ttttaatcga aatattgctt ttcacgtgga gtgtgactgt ggggaattag caaaaagtgt
     5581 ttatcaaaaa aattactttt actgtcctaa atgccatcgc cgatacgggg tacatggaac
     5641 atagaaacga aatagtgcgg tagaaaagcg aatgaatgta gtatatttct acaaatcatt
     5701 cgctttcttt attttataaa tagttggtcg agagatcccg ttttcttttg caatctctgc
     5761 aactggaatg ccttgcttta acatgtccac cacggtttta aaaactaagc gtttttgcgg
     5821 atctgttgag ttaatagaat actcgattgg tcgtccctta tatcggccgg ctttttgagc
     5881 caataatact ccttgccgct gacgttcacg aattttctca cgctcgtttt gtgcaatata
     5941 acttagcagt gataaaaaca tatcgaacat agcggtatcc aggagttcat ttccagtatt
     6001 aaaatctaaa aactgtgcat ctaatatttt taacgctact tttttctgtt tcataaaagc
     6061 aactgtattt tttatatctt cataatctct tcctaaacga tctagtgatg taacgatgac
     6121 gcaatcacct tcacggatat aatttagtaa tttttgaaac tcaggacgct cagtatcttt
     6181 accactggct ttttcttgga ataatttatc aatgttaagt ccttccatca ttgccaattg
     6241 tcgatccaaa ttttgatctt gtgaagatac tcgaatatag ccaatttttc ccataaaaaa
     6301 ctccttttta aaagtgatat aatttacatg taaaatttac ttgtagtttt actgctatca
     6361 tagcattttt aaaaataaat tttatgtaga cttaaaattt acttcatgga gggtttaaat
     6421 ggatgaaatc aaaagtaaca aaaaagcatt tggcatttat ttaggagaac ctagtacaga
     6481 acaattaaac caattttttt atttgcatga aaaagattta gaattgatgg agactatacg
     6541 acgaccagct actagattag gttttgcact acagttagtc tcactacgtt ttttaggaac
     6601 ttttatatta gatcttacca atattcccca aaatgtattg agttatgtag caactcaact
     6661 aaatattgat cctggagaaa ttatctacta tagcaggaga cagaccaggt atcagcacac
     6721 aaaattgata aaatcacatt atggatatca agatttcaac caaccggaaa tagaaacata
     6781 cctagctaac tggcttttaa atagagcgct gtatacaaca gaatcttcag atatgttgtt
     6841 tgatatgctt ctaaaaaaat gtctggatga aaagattgtt ttacctggaa ttactacttt
     6901 ttctcgtttt gttgctagtg caattgaaaa agctgaagag catttatacc gacaattgtc
     6961 tttggttcca actgaagatg agagaaagcg gcttctcaat catttagaat taattggaac
     7021 tccagtttat ggggcaataa tcaaaatgga cattttgaga accccgttag ctgacgatag
     7081 tcataaagaa atagccagag gttttgaccg actaaagcaa tttcaagctt tctctactga
     7141 aaaatggaag atggaatcta ttcctgaagg gaaaataaaa gttcttgcaa attatgcatt
     7201 taaagctaaa gctcaattaa ttcaacgaat gagtgaacag aaaaaactgg cgttgttagt
     7261 ggcatttatt ttcatataca aacgtaaggc tatggatgaa caaattttag cactatctaa
     7321 tttttttgaa actgttttta gacgcgccaa aaataaggaa tctaaagaac gtcttcgtac
     7381 cataaaagat ctagatcgag ccgcatcgac actttcagat attgtagatt tagtaatgtc
     7441 cagtcattct gatactaatg aactatccct tcgcgatcgt atcttagaaa aatattctga
     7501 ggaaaatgtt accgaagctg tatcgcaagt tcggctattg gttaaaaatg atcgtgaacc
     7561 aatagccatt tctgaacttc tgaacactta tcgaaagttc agaaaattta ttccggatat
     7621 tttaactaca ctaacatttg acagcaatga ctacggtaac aactgtaagg aactctggac
     7681 cttcattaat aatagatttc ctaagccgat tacttacaaa atgtttggag ctattaaaca
     7741 tactcttcca aagaaatgga tttattacat tcatgaatac cctgaacaaa ctaataaatg
     7801 tgtattgatt gcagctgtcg aattgttaat tcaatcttta aaacgccatg atatttatat
     7861 tcctcagagt gtcttatata acaatcctat ggattgcttg attgatgaga aaacttgggt
     7921 tcaacaaaaa tacgttttat tagaacaact agatcttcct tcttcagcaa atgaaatgtc
     7981 tttatggtta aagaacgatt tggaactgtc ttataaagaa gcgctaaatc gttggcctac
     8041 ttctaaaatg gccagaatag aaaatgcaga tcgcctggtt gtctctagac ttaaaaagac
     8101 atatgaaaaa aaggaagata agtcttttag acgtcgcatt cagcagttac ttcctaaaat
     8161 tgatttgtca gatttgttat tggaagtaaa ccaacagtta aatcttactc aatcatttaa
     8221 ccacttaagt gacaaggaca ccaaaatgaa agacttggat attagtttat tagcagttct
     8281 tttatcagag tcctgcaata ttggtttttc cccggtttca aaagaaggat ttgattcttt
     8341 aaagtatgat cgtcttgtct atgtcaatca tcattatatt cgagtggata cactttcagg
     8401 agctaatcaa aaaataatac atgcacacag gaaacttgcc acctctaaga tatggggtga
     8461 tgggcatatg gcttcagctg acggtattcg ttatatcaca ccacaacgtt ccctatattc
     8521 tagaagtaat ccaaaatatt ttggccgtgg tcgtgggatc acattctaca attttgtctc
     8581 tgatcaatat atagggttcc acggaatggt cgtttcaggg acactgcgag attcgcttta
     8641 ccttctagaa ggattcctca atcaacccag cgaattaact cctaatcaga tcatgactga
     8701 tacagctggt tatagcgata tgatttttgg tctctttggc ttattagggt ttcaatttag
     8761 tccaagaatt gccaatgata aaggaaccaa actttggcga atcgacgcta acgctcacta
     8821 cggggaactt gatacgttgt ctcaaaattc aattaatatg gaactgattt atacccattg
     8881 ggaggacatt cttagggttg caggttcgtt gaaatctgga aaagtgaatg caacagaatt
     8941 aacgaaatct cttcaacgga atgggcaacc cacgccttta ggaaaagcaa taactgagta
     9001 tggaaaagtt tataagacga aacatcagct tcgttatatt tctgacgaaa tatatgcacg
     9061 gcaaatttta gaacaattga ataaggggga agctcggcat gccttatgcc gtaatatttt
     9121 ttatggacga aaaggaaaac tctaccagac ttattatgat gggatggaag agcagctcaa
     9181 tgcattagga ttagttacta atgccatcat atattggaac tcactgtatt tagaaaaagt
     9241 atttgaacaa atgaaagctg aaggattcga ctgttccgaa gaaatgatac gcaggcaatc
     9301 tccactaatt agtgatcaca tcaattttgt tggtaagtat acgtttagat acgaagctga
     9361 attatccaac ggtaagttac gtccattgaa tgtagaagaa ttctagcaat gttcgggttt
     9421 tgcatgctac cgcactattt cgtatgactg ttccagcgaa tcacttcgaa tagaaaaaga
     9481 gaaaaacacg agaacagcgt taaatcaacg tttcttgtgt ttttactttt gtgctaccgc
     9541 actatttcgt ttctatgttc catgtacccc tttatcaaga agcgcaaaca cgccaaattg
     9601 ccgattggtt gatcggcatg aacgcaagcc ctttatatac cttgaattta caacaaaaag
     9661 gcgtacaagg tacattttca ttaggacgtg tacaaacacc gactttatat ctcatttatc
     9721 aacgccagga agcaatagaa aactttaaaa aagaaccttt tttcctgaat aattcatagt
     9781 tttactaata gtctttccta acattgattt aatagtctt
//
LOCUS       DADXTH010000091         9787 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.091, whole genome shotgun sequence.
ACCESSION   DADXTH010000091 DADXTH010000000
VERSION     DADXTH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.091"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..195)
                     /locus_tag="IWI97_002390"
     CDS             complement(<1..195)
                     /locus_tag="IWI97_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463867.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="IWI97_002391"
     CDS             complement(211..825)
                     /locus_tag="IWI97_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBC2463868.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="IWI97_002392"
     CDS             complement(972..1655)
                     /locus_tag="IWI97_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBC2463869.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="IWI97_002393"
     CDS             complement(1764..2222)
                     /locus_tag="IWI97_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBC2463870.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="IWI97_002394"
     CDS             complement(2306..4069)
                     /locus_tag="IWI97_002394"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBC2463871.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="IWI97_002395"
     CDS             complement(4290..5750)
                     /locus_tag="IWI97_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463872.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="IWI97_002396"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="IWI97_002396"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBC2463873.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="IWI97_002397"
     CDS             complement(6907..8334)
                     /locus_tag="IWI97_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2463874.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="IWI97_002398"
     CDS             complement(8365..9579)
                     /locus_tag="IWI97_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBC2463875.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2899 a         2040 c         1624 g         3224 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttt
//
LOCUS       DADXTH010000092         9702 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.092, whole genome shotgun sequence.
ACCESSION   DADXTH010000092 DADXTH010000000
VERSION     DADXTH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.092"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            34..657
                     /locus_tag="IWI97_002399"
     CDS             34..657
                     /locus_tag="IWI97_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463876.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="IWI97_002400"
     CDS             1107..2156
                     /locus_tag="IWI97_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2463877.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="IWI97_002401"
     CDS             2153..3034
                     /locus_tag="IWI97_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2463878.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="IWI97_002402"
     CDS             3037..3807
                     /locus_tag="IWI97_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2463879.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="IWI97_002403"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="IWI97_002403"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBC2463880.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="IWI97_002404"
     CDS             5118..6281
                     /locus_tag="IWI97_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBC2463881.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="IWI97_002405"
     CDS             6398..6808
                     /locus_tag="IWI97_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2463882.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="IWI97_002406"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="IWI97_002406"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBC2463883.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="IWI97_002407"
     CDS             9381..9665
                     /locus_tag="IWI97_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463884.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3371 a         1645 c         2086 g         2600 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa ac
//
LOCUS       DADXTH010000093         9435 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.093, whole genome shotgun sequence.
ACCESSION   DADXTH010000093 DADXTH010000000
VERSION     DADXTH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.093"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(24..185)
                     /locus_tag="IWI97_002408"
     CDS             complement(24..185)
                     /locus_tag="IWI97_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463885.1"
                     /translation="MENINSISENTATTTTPDEVRTKTKAYQDFINVLSQIIEKHGAE
                     VLNEINSAK"
     gene            complement(755..1177)
                     /locus_tag="IWI97_002409"
     CDS             complement(755..1177)
                     /locus_tag="IWI97_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBC2463886.1"
                     /translation="MKKINLKDYYSHITVDTYIDIPDEVFNIFEEYRKAEQAYQSRIR
                     YHKAYYSLDRGDGIEHTALFVSLSPDEIYERKLTTEQLHAAIATLPDKQAKRIYAHYF
                     LGMSKAAIAKAEGTSASSVKDAIRRGLCSLEKYLKNNF"
     gene            complement(1603..2433)
                     /locus_tag="IWI97_002410"
     CDS             complement(1603..2433)
                     /locus_tag="IWI97_002410"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBC2463887.1"
                     /translation="MVLDFIEILKVIFLGIVEGITEWLPISSTGHMILADKFITLNMS
                     EAFKEMFFVVIQLGAILAVVVMFWNKMFPFQLKDKSQPLVKKDTFSLWFKVAVACIPS
                     GIMGILFDDYLDAYLQTPIVISIMLIFYGLLFILIENWNKKRTPTTMALSDISYKTAI
                     LIGAFQVLSLIPGTSRSGATIIGALLIGVSRVAAAEFTFFLAVPTMLGASAFKLLKFG
                     FDFTSAELLALVLGMAVAFAVSVLVIKFLMNFIKKHDFKVFGWYRIVLGILVVLITVM
                     "
     gene            complement(2433..3182)
                     /locus_tag="IWI97_002411"
     CDS             complement(2433..3182)
                     /locus_tag="IWI97_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2463888.1"
                     /translation="MLNLISCELLKLKRSKMVLISVAGVLSTPLLMLIEALQTHFDKP
                     EIIFTLSDIYSDSVLYIMLLVNMMIYVAIAAYLFSREYTESTLKTILPIPISRTKLLI
                     GKFCTLLLWIVMLTLVTWAGIFIACGLYHAVFTLEGYSLLVAIVWLPKFLFGSILMFL
                     TVSPFVFVAMKTKGFVAPMIGSAVIVMGSAALSNQEWGALYPWTATYFLVQGKLQSTG
                     YPTLLSVSIIILVSAVGFLMTFHHFKKEDLK"
     gene            complement(3175..4092)
                     /locus_tag="IWI97_002412"
     CDS             complement(3175..4092)
                     /locus_tag="IWI97_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2463889.1"
                     /translation="MDYIIETENLTKRYGTATVVDKVNLHVPTGKIYGLLGRNGAGKT
                     TAMKMMLQLTLPTDGTVRLFGTNYKENIHTLYSKVGSIIETPGFYSNLTGYENLQILA
                     KLRGGVSKSGVEKALEVVGLHKEKRKVFSDYSLGMKQRLGIAAAIMHEPELLILDEPI
                     NGLDPIGIVEIRSFLSELSHNHGITIFISSHVLSEIEQIADIIGVMHEGHLVEEVNIS
                     ELHKRNRKYIQFDLSDSEIAGKILESHYHMTDFTVQDGTVRIYDFSQSVGEINREFAR
                     NGLLVTRIDDSEENLEDYFSKLIGGGGIA"
     gene            complement(4275..4889)
                     /locus_tag="IWI97_002413"
     CDS             complement(4275..4889)
                     /locus_tag="IWI97_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2463890.1"
                     /translation="MEFNEKLQQLRTGKNLTQEQLAEQLYVSRTAISKWESGKGYPNI
                     ESLKCISKFFSVTIDELLSGEELITLAETENRSNLKKIYNYIYGILDMMAVAFIFLPL
                     YGNSVGGYVYAVNLLSFTATTPFNLAVYWSAFAALIVIGIAKIILTHLDKETWGGIAT
                     KCSLGLTALAICFLAAAREPYITALMFLLFVAKIVVWIKQNGTK"
     gene            complement(4879..5205)
                     /locus_tag="IWI97_002414"
     CDS             complement(4879..5205)
                     /locus_tag="IWI97_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBC2463891.1"
                     /translation="MIFERSMILCLVVYNFVLVERNLLLFVIPYIIIIYSLRKEVDIM
                     DYISVKAASEKWGISERRIQKLCEENRIDGTEKFGRAWMIPKDAEKPVDGRMKTRNKQ
                     EENHGI"
     gene            5242..5601
                     /locus_tag="IWI97_002415"
     CDS             5242..5601
                     /locus_tag="IWI97_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463892.1"
                     /translation="MYQDERKLDFKPLGIAIKKAREAKGWTQEYLAQLVDLTPRSIMY
                     IENRGQHPRLNKFYLITTLLDISVDQFFFPCNEDGDNNRRKQVDVLLNDMEEKELIVM
                     EAGLVALYDRPSSRDGI"
     gene            5561..5782
                     /locus_tag="IWI97_002416"
     CDS             5561..5782
                     /locus_tag="IWI97_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463893.1"
                     /translation="MLYMTDHQAEMEFEDGVDNVKISGLKFFTAKDGQNTIPELMAMS
                     GVTYQKPNEQIDLLMVADPSTDVIIEEDE"
     gene            6019..6285
                     /locus_tag="IWI97_002417"
     CDS             6019..6285
                     /locus_tag="IWI97_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBC2463894.1"
                     /translation="MDYGFISTIVRSELFMMQLDSVLVSGAQPNVLSKEIDSFNFMIP
                     ILVQEQQKIGSFFKQLDDTIALHQRKLDLLKEQKKGFLQKMFAK"
     gene            6314..7291
                     /locus_tag="IWI97_002418"
     CDS             6314..7291
                     /locus_tag="IWI97_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBC2463895.1"
                     /translation="MLNKSIFISYAWEKDEPLDKKVKNFSQWLAIYLQSWGFRVHLDV
                     LENHPGTKLDEFMKNGIDSSHFVICICTQTYLKKISDSATGVYNEIELIKKQAKSPFI
                     IAIIDIESFTDLPDFFEGKYISQLKFDTPFSQENKKGLFELISTIRDELFLNIKVETK
                     PDAQDRIERYYNSVEKIKFLNDSANLMNFKPQSEGEISFQYLLNGGDFKLGISPMEFI
                     THWTSAGSDTIYSYKKVNKMMRIHNFENFDRINKPADIDFDKVVDINWGASLREGDGI
                     VWINDKNYLAIGKIIQIYLDSEDEYKSMVTLAYKILNPIDLTDDFIDSK"
     gene            7472..8305
                     /locus_tag="IWI97_002419"
     CDS             7472..8305
                     /locus_tag="IWI97_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBC2463896.1"
                     /translation="MPYHIFINRESGNDNISVFYDEPTFVVIANKDDLPAIQLIEESQ
                     KPGIYILLGDNKRYVGQASNSIFSRLQQHNTNKPWWNKLIFFGREDGHLSKAQLDLLE
                     RLIIEKMNTANIDLDNSTQGNKSYIDKLSKFSALSLWNKIDKLLNDVANIDLFDSEHI
                     QTEDNSDNSTLNSHISIIFGESLYTGKSYRDVFTQLVSNLVLSAELDKLIPLMSPNEP
                     TTVQILGNREHISAKGAKLTKPIELTKYHMYVNFSKIGLYNQIKKLAELTGKKVIFER
                     W"
     gene            8311..8901
                     /locus_tag="IWI97_002420"
     CDS             8311..8901
                     /locus_tag="IWI97_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2463897.1"
                     /translation="MVQQIVLPIKDSNILKMVQDTLLDSFHAGRRNYTIFQVGKATLL
                     RVSDVMKLKKSDVFNPDGTVKQTAFIHDQKTGKANTLYLKPVQQDLLIYHDWLVQQNL
                     NSEWLFPSTTHPDRHITEKQFYKVMARVGDLLGINYLGTHTMRKTGAYRVYTQSNYNI
                     GLVMHLLNHSSEAMTLTYLGLDQASRETMLDQIDFG"
     gene            9029..9289
                     /locus_tag="IWI97_002421"
                     /pseudo
     CDS             9029..9289
                     /locus_tag="IWI97_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         3076 a         1760 c         1696 g         2903 t
ORIGIN      
        1 caataaatta ttggaaaaca tctttactta gcactattta tctcatttaa aacctctgca
       61 ccatgttttt caataatttg cgaaagcaca ttgatgaaat cttgatatgc ttttgttttt
      121 gtacgaactt catcaggtgt agtggttgta gcagtatttt cagatatgct gttgatattt
      181 tccattcgca atcacctcca agtgtatcgc ttatatagat tataagcagt tatttcattt
      241 atgaaaagtt tgtcaccata gttaaaaatg caaaaccaac cgcactgtaa atttatagtg
      301 cgattggttt ttgattacat catattgtcg tatttgccac gcaccccgac aaaggggata
      361 ttttgagttg ctgttggcac agctatcata gccccatgat accgtagctc gtcctttcaa
      421 aagccacgca taccgtagga actccctccg ttttaatcgt attgagggct aaacgtatcg
      481 aagtatcatt atcccttatg tgggtcattg cggacgagtg tgccacactc ggtctaacag
      541 tccatttatc gctcgggcgg tagtataatc acctccccat accgctgtta tggcgatgtc
      601 tgttaagtcg ctttgccttt cggctcacat aagtaatgta ctcaaaatat cgtatattca
      661 gttgtcaagg aacaattatg ctgtttggta tataagagaa aaaaacttcc tcttatatac
      721 ctttagaaaa agaggggtaa acgacgtggg tagattagaa attatttttc aaatactttt
      781 ctaaactaca tagtcctctg cggattgcat cttttacgct acttgctgat gtaccctctg
      841 ccttggcaat agctgctttg ctcattccta aaaagtagtg agcataaata cgttttgcct
      901 gcttatccgg cagggtggca atggcagcat ggagctgttc ggttgtgagc ttacgctcgt
      961 aaatctcatc gggtgagagg gaaacaaaca aggcagtatg ttcaataccg tcaccacggt
     1021 caagagagta gtaggcttta tggtagcgta tgcggctttg gtaggcttgc tctgccttgc
     1081 gatattcttc aaagatgtta aatacctcat cgggaatgtc aatatatgtg tccacagtta
     1141 tgtgggaata gtaatctttc aagtttattt ttttcatggt tgtaccatcc tttcaaattt
     1201 tggtttagtg cgaatttgaa aggacggtgg gggtgaacga caccgggggt ctatatacgc
     1261 aaaaatgcca gactgaaatt cataaaatct caatcgggca taggtgtatt tgtttcgtat
     1321 tatgtagttt ttattttcgt gttcttgccc gtaatcgtcc cacaatgtgg taaggatttt
     1381 tttgataaat taccaagtgt tagggcaaaa gaaaacacgg cgatacctcc ttggataccg
     1441 ccgtgttcct atcagtcgtc attggtttgt gtaaacgcaa agaaacggcg gctctgaaaa
     1501 tcaaagccac cgtttcatag gcgtgaaaat atgtctgctg tacgacggct tttttctttt
     1561 gccgtcgtgc ggcagattat tgcttatgga attgtttttc tatcacatta ctgttataag
     1621 tacaacaagt ataccaagca caattcgata ccaaccaaac accttaaaat catgtttttt
     1681 gataaagttc attaggaatt taattactaa gacagatacc gcaaatgcca cagccatacc
     1741 gagaacaaga gcgagcagtt ctgcacttgt aaaatcaaac ccgaatttta acagcttaaa
     1801 agcacttgct ccaagcatag taggtactgc gaggaaaaaa gtaaactctg ctgctgccac
     1861 tcgtgaaact cctatgagta aagcacctat aatcgttgct cccgaccgtg atgtgccggg
     1921 tataagtgat aacacttgaa atgcccctat caagattgct gttttatagc tgatgtcaga
     1981 aagtgccata gtagtaggag tacgcttttt attccaattt tctatgagaa tgaataacaa
     2041 accataaaag attaacatga ttgaaatcac aatgggggtt tggaggtaag catccaaata
     2101 atcatcaaat aaaatcccca taatacctga cggtatacaa gccactgcaa ccttgaacca
     2161 caacgagaaa gtatcctttt taacaagtgg ctgcgattta tcctttagct gaaatggaaa
     2221 catcttgttc caaaacatta caaccactgc gagtatagct ccaagttgaa taacaacaaa
     2281 aaacatttct ttaaatgctt cgctcatatt aagtgtgata aatttgtccg caagaatcat
     2341 atgtcctgtg ctgctgatag gtagccactc agtaataccc tcaacaattc caagaaaaat
     2401 aacttttaaa atttctataa aatcaagtac cattatttca aatcctcctt tttaaaatga
     2461 tgaaaggtca taagaaaacc aactgctgat acaagaataa taatagatac agacagcagt
     2521 gtaggatagc cggtactctg aagtttaccc tgcaccaaga aataggtggc tgtccacgga
     2581 tacaacgctc cccattcttg atttgaaagt gcggcacttc ccatgacaat cacggcagag
     2641 ccaatcatcg gggcgacaaa tccttttgtt ttcatagcaa caaacacaaa aggagaaact
     2701 gttaaaaaca tcaggatact gccaaacaaa aacttgggga gccatactat tgccactagt
     2761 aagctatatc cctccaatgt aaagacagcg tgatatagcc cacaagcgat aaatatacct
     2821 gcccatgtta caagggtgag cataacaatc caaagaagca gggtgcaaaa ttttccaatt
     2881 aatagttttg tccttgaaat gggtatgggc aatatggttt tgagtgtgct ttctgtgtac
     2941 tctctgctaa acaagtaagc tgcaattgcc acatatatca tcatgtttac cagcagcatg
     3001 atatacagta cactgtcgct gtatatgtca gacaaggtaa agataatctc cggcttatca
     3061 aaatgtgttt gcaaggcttc tattaacatc aaaagtgggg tagataatac ccctgccaca
     3121 ctaattagca ccatttttga acgctttagt tttaataatt cacaagaaat aagattaagc
     3181 aataccgcca cctccaatca gtttagaaaa gtagtcctct aagttttcct cactatcatc
     3241 tatccttgtc acgagcagtc catttcgtgc aaattctcga ttgatttctc caacactttg
     3301 gctaaagtca taaattctca ccgtaccgtc ctgcactgta aaatccgtca tgtggtagtg
     3361 gctttctaaa atctttccag ctatctcact gtcagataaa tcaaattgaa tgtattttcg
     3421 attccttttg tgaagctcgg atatattcac ttcctctact aaatgcccct cgtgcataac
     3481 gccgataata tctgctatct gttcaatctc gcttaaaaca tggcttgaga taaaaatggt
     3541 aatgccatga ttgtgactaa gttcagataa aaatgagcgt atttcaacta ttccaatggg
     3601 gtcaagtccg ttaatcggtt cgtcaagtat taaaagctcc ggctcgtgca taatggcggc
     3661 ggcaatacca agccgttgct tcattccgag ggaataatcg gaaaagactt ttcttttctc
     3721 cttatgcagc cccacaactt caagcgcttt ttctactcca cttttagata ctccccctcg
     3781 cagtttagcg agaatttgca aattctcata ccctgttaaa ttactgtaaa atcccggtgt
     3841 ttcgataata gagcctactt tgctataaag ggtatggata ttttccttat agtttgtgcc
     3901 aaacaagcgt accgttccat ccgttgggag agtaagctgc aacatcattt tcattgctgt
     3961 ggttttgcct gctccgtttc tgccgagcaa accataaatt ttgcccgttg gcacatgaag
     4021 attgacctta tcgacaacgg tggctgttcc gtatcgcttc gtaagatttt ctgtttcaat
     4081 gatataatcc atatttgatt ttcctttccg ttggttgctg ttcttgtttt cactgaccac
     4141 aatcttatta tcacaggagc tatatcaaaa gccaagaaac ctaccgtcac atcgcctaca
     4201 tctatcatgt caacatgaaa aaaagacccg acacttatcg tgtcagagcc tatttcaagg
     4261 gatttatatc gtttttactt tgttccgttc tgcttaatcc aaacaactat ttttgcgaca
     4321 aacagcagaa acataagtgc tgttatatat ggttctcttg ctgctgctaa gaaacagata
     4381 gccaaagcag tgagtccaag ggagcatttt gtagcaatgc cgccccatgt ttctttatct
     4441 aaatgggtaa gtattatttt agcaatccct attacaatca aggcggcaaa agcactccag
     4501 tagactgcaa gattgaatgg tgtggtggct gtaaatgata gtagattgac cgcataaaca
     4561 taaccgccaa cagagtttcc atataacgga agaaatataa acgcaactgc catcatatcc
     4621 aatattccat aaatatagtt gtaaatcttt ttcaagttgg aacgattttc agtttcggca
     4681 agtgtaatca gttcttcgcc cgatagtagt tcatctatgg tcacagaaaa gaatttagaa
     4741 atacacttga gcgactcaat gttagggtaa cccttgccgc tttcccattt tgaaatggct
     4801 gttcttgata catataattg ctccgcaagt tgttcctgcg ttaagttctt tccagtccta
     4861 agctgttgta gcttttcatt aaattccatg attctcctcc tgtttgttcc ttgttttcat
     4921 acgtccatca acgggttttt ctgcatcttt tggtatcatc catgcacgac caaatttttc
     4981 agttccgtca atgcgatttt cctcacataa tttttgtatc cgtctttccg atatgcccca
     5041 tttttcagaa gcggctttca cagaaatgta atccataata tcaacttcct ttcgcaaaga
     5101 gtatataatt attatatacg gaatgacgaa taatagcaag tttctctcca ctaatacgaa
     5161 attatacact actaaacata aaatcatact tcgttcaaat atcatcaccc gaaatgtaca
     5221 atgatatagg gtgatattaa aatgtaccaa gatgaaagaa aacttgattt taagccgtta
     5281 ggtatagcga taaaaaaagc tcgggaagca aaagggtgga cacaggaata tcttgcccaa
     5341 ctggtagacc ttacgccacg ctctattatg tatatagaga acagggggca gcacccaagg
     5401 cttaacaaat tttatctcat tactaccttg cttgacatct ctgtagacca gttctttttt
     5461 ccatgcaatg aagatggcga caacaatcgc cgcaaacaag ttgatgtact gttgaatgat
     5521 atggaagaaa aggagcttat cgtgatggaa gcaggacttg ttgctctata tgaccgacca
     5581 tcaagcagag atggaatttg aagatggtgt cgataacgtc aagattagcg gtctgaaatt
     5641 ctttaccgca aaagacggac aaaacacaat ccctgaattg atggcaatgt ctggcgtgac
     5701 ctatcagaaa cctaatgaac agatagattt attgatggta gcagaccctt ctactgatgt
     5761 aataatcgag gaggatgagt gaaatgccaa agaaattaga tatttttgtt gagaaagttt
     5821 ttgttccatt cattagctca atttgtctag ataagcgatt agaatcacat atcaaacgct
     5881 aaaagtatag actctcacta taaaggtggg ggtctttttt gcggaaacat ttttttacca
     5941 ccccaccctt acactttgtg agtgctttaa tatccttatc aaaaccagcg agttggttat
     6001 tttcagtctg tagaaaatat tgactacggg tttatttcca caatcgtccg ttcagaatta
     6061 ttcatgatgc aacttgattc tgttctagtt tcaggcgctc agccaaatgt attgtcaaaa
     6121 gaaattgatt cgtttaattt tatgattccc atattagttc aggaacaaca aaaaatcggt
     6181 tcattcttta aacagctaga tgacactatc gctcttcatc aacggaaatt agatttattg
     6241 aaagaacaga aaaaaggctt tttacaaaaa atgtttgcaa agtgattatt aattacataa
     6301 ggagggtgta gaagtgttga ataagtctat ttttatttca tatgcttggg aaaaagacga
     6361 gccactagac aagaaagtta aaaatttttc acaatggtta gcaatttatt tgcaatcttg
     6421 ggggtttcga gtccatcttg atgtattgga aaaccaccct ggaactaaac ttgatgaatt
     6481 catgaagaac ggtattgata gttctcactt tgttatttgt atttgtacgc aaacttattt
     6541 aaaaaaaata agcgattcag caaccggcgt atataacgaa atagaattaa taaaaaaaca
     6601 agctaaaagt ccatttataa ttgccataat tgatatcgaa tcatttactg atctgcccga
     6661 tttttttgaa ggaaagtata tcagtcaatt aaaattcgat acaccgtttt cgcaggagaa
     6721 taaaaaagga ctatttgaat taatatcaac tatacgtgac gaactatttc taaatatcaa
     6781 ggtggaaaca aaacctgatg ctcaagacag aattgaaagg tactataata gtgttgaaaa
     6841 aataaaattt ttgaatgatt cagccaattt aatgaatttc aaaccacaat cagaaggcga
     6901 aatctctttt cagtacctac ttaacggcgg agactttaaa ttaggtattt caccaatgga
     6961 atttataact cattggacaa gtgcaggaag tgatacaatt tactcctata aaaaagtcaa
     7021 taagatgatg agaattcata actttgaaaa ttttgacaga ataaataaac ccgctgatat
     7081 tgactttgat aaagtagttg atattaattg gggtgcatca ttgagagaag gagacgggat
     7141 cgtatggatc aatgataaaa actatctagc gataggtaaa ataattcaaa tttatttgga
     7201 ttccgaagat gaatataaaa gtatggtaac tcttgcttat aagattttaa acccaattga
     7261 tctgacggac gatttcatcg atagcaaata gcctatatat ttattggcgc tcataaatat
     7321 ataaaacaaa agattctata tttttacagg gtctataatc agataataac tcctataaaa
     7381 aactattaaa acagtccttt taatctaact gtcagattaa aggggctgtt tatttatttt
     7441 aatgatataa taaccctaaa aggaggacgt tatgccatac catatattta ttaatcgaga
     7501 atctggaaat gataatattt ctgtttttta tgatgaacct acttttgtag ttattgccaa
     7561 taaagacgat ttaccagcaa ttcaattaat tgaggaatct caaaaaccag gtatatatat
     7621 attactagga gataataaac gttatgtagg tcaagcctct aattctattt tttctaggtt
     7681 acaacaacac aatacaaata aaccatggtg gaacaaatta atattttttg gacgtgaaga
     7741 tgggcatcta tctaaagcac aactagatct tttagaacgt ttaatcattg aaaaaatgaa
     7801 tactgctaat attgatttag ataatagcac tcaaggaaat aaaagctata ttgataaatt
     7861 aagcaaattt tcagctctgt cattatggaa taagattgat aaactattga atgatgttgc
     7921 caatatcgat ttatttgatt cagaacacat tcaaactgaa gataatagtg acaactcaac
     7981 tctaaatagt catatatcaa tcatatttgg agaatcatta tatactggga aatcatatag
     8041 agatgtattt acgcaattag tttctaattt agtattatcc gcagagttgg ataaattgat
     8101 tcctttaatg agtcctaatg aacccactac agtacaaata cttggcaata gagaacatat
     8161 atcagctaaa ggggcgaagt tgactaaacc aatagaacta actaaatacc atatgtatgt
     8221 taatttttca aaaataggct tatacaatca aatcaaaaaa ttagctgaac tgactgggaa
     8281 aaaagttata tttgaaaggt ggtaattcat atggttcaac agattgtttt acctatcaaa
     8341 gattcaaata tcttaaaaat ggttcaagat acactccttg atagctttca tgctggacgt
     8401 cgtaactata cgatattcca agtggggaaa gccactctct taagggtaag tgatgtcatg
     8461 aaacttaaaa aatcagatgt ctttaatcct gatggaacgg ttaaacaaac cgcttttatt
     8521 catgatcaaa aaacaggaaa agcgaatacg ttatatttga aaccagtaca acaagattta
     8581 ttaatttatc atgattggct ggtccaacaa aatcttaatt ctgagtggtt gtttccctca
     8641 acgactcacc cagaccgtca catcaccgag aagcaatttt ataaggtcat ggcacgtgtt
     8701 ggtgatcttt taggaatcaa ctatctaggg acgcatacca tgcgtaaaac aggcgcttat
     8761 cgtgtttata cgcagtcgaa ttataatatt ggtttagtca tgcatttatt aaaccattca
     8821 agcgaagcca tgactttaac ctatcttggg ttagatcaag ccagtcgaga aacgatgtta
     8881 gaccaaatag attttggata atgtttttta tttaaaaaga gggacaatca ttatgttaaa
     8941 aaattaggtt ctgttgcaaa gttttccaaa aaatctattt tagtttaaaa ttgagaaaaa
     9001 agacagagag gacagagtaa tgaatcattt tggtgagccg aaagcaattg tgaccgataa
     9061 agcaccttct cttggctccg cctttagaaa gttacagagt gtgggtttat atactaagac
     9121 agagcaccga actgtgaagt atcttaacaa tttaatagaa caagaccatc gacctattaa
     9181 acgacggaat aaattttatc aaagtctccg tacagcctct tccacgatta agggcatgga
     9241 gaccattcga ggaatatacg ccgtttgtat aattaagcga gacaaataaa aaagccattt
     9301 atgttacact caaattgtaa tacccgctaa agcatacaag gagagtgaac ataaatgact
     9361 tatacccatc ttacaccaaa cgagcttgta atgatagaag catattttca tcaagaaact
     9421 ccggttgcta tcgtt
//
LOCUS       DADXTH010000094         8926 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.094, whole genome shotgun sequence.
ACCESSION   DADXTH010000094 DADXTH010000000
VERSION     DADXTH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.094"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..1095
                     /gene="phnW"
                     /locus_tag="IWI97_002422"
     CDS             4..1095
                     /gene="phnW"
                     /locus_tag="IWI97_002422"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBC2463898.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1088..1873
                     /locus_tag="IWI97_002423"
     CDS             1088..1873
                     /locus_tag="IWI97_002423"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBC2463899.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(2219..3910)
                     /locus_tag="IWI97_002424"
     CDS             complement(2219..3910)
                     /locus_tag="IWI97_002424"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBC2463900.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4332..5279)
                     /gene="arcC"
                     /locus_tag="IWI97_002425"
     CDS             complement(4332..5279)
                     /gene="arcC"
                     /locus_tag="IWI97_002425"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBC2463901.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5394..6413)
                     /gene="argF"
                     /locus_tag="IWI97_002426"
     CDS             complement(5394..6413)
                     /gene="argF"
                     /locus_tag="IWI97_002426"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBC2463902.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6504..7733)
                     /gene="arcA"
                     /locus_tag="IWI97_002427"
     CDS             complement(6504..7733)
                     /gene="arcA"
                     /locus_tag="IWI97_002427"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBC2463903.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            8194..8895
                     /locus_tag="IWI97_002428"
     CDS             8194..8895
                     /locus_tag="IWI97_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBC2463904.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         2617 a         1789 c         1609 g         2911 t
ORIGIN      
        1 catatgtata aattattaac tcctggacca ttgacgactt ctgaaacagt gaaaaatgct
       61 atgctgatcg atcattgtac ttgggatcaa gaatacaaag aaattacaca atggataaga
      121 aaaaaattat tggatcttgc tcaagtatcg gaaggaatat acacaacggt cttaatgcaa
      181 ggtagcggct cctttggggt agaatcggta ttaagttctg tgatcaggcc agaagatact
      241 gtactgattt gttcaaacgg cgcatatgga caacgaatga tcaacatgtg cgaatgtctg
      301 aaactgaact ataccgttta tgaggtatcg gaacatcaaa tcctccaagc tcatgtgatt
      361 acacagctga ttgaagagaa tccctctatc tccgcactag caatggtcca tagcgagact
      421 acttccggta ttttgaataa tttggaagag attgctgctg tcacacaaga aaaagatctt
      481 ttgtttatag tggacgcgat gtcaagcttt ggtgggatac cgattcgtgt agccgaacta
      541 gggattgatt ttttgatttc cagctcaaac aaatgtattc aaggtgttcc aggcttttct
      601 tttgtacttg ctaataaagc ggaactagaa aagcgaaaaa atgtgagtcg aagtttgtca
      661 cttgatctat atgaccagta ccgcgtgatg gaaaacgaga atggcaaatg gcgtttcact
      721 tcccctaccc atacggtttt ggcttttcaa caagcgctga aggaactaga ggcagaagga
      781 ggaatcgaaa ctcgatattc acgttatctc gccaataatc atgtactacg acaaaaaatg
      841 acagagatgg ggttccagcc tttcattcaa gaatatcagg ggccattcat cacctctttt
      901 ctttacccta aggactggcg atttgatttt aatcattttt atcagtattt aaagcaaaac
      961 ggatttgtga tctatcccgg gaaaatcagc gatcatgaat gttttcgtat cggaaatatt
     1021 ggcgagatct atccggaaga tattttggaa ctgacagcaa ttatcaaaga ctataaggag
     1081 aacgttaatg attaaaactg taatttttga ttgggcagga acaactgtgg actttggctg
     1141 tatggctcct gtccacgctt ttagaaatgc ttttctagaa aaaggaatcc aactgactga
     1201 caaagaaatc cgcgaaccga tgggaaaatt aaaatgggat catatccaac agcttctttc
     1261 cctcccttct gtcaaaaaac aatggatagc tatttatgga caactacctc aggaaaaaga
     1321 ggtggaggaa ttgtatcaac aatttgaaca ttatctgttt aaagatttgg tcaagcattc
     1381 taccttgaaa cccgacacat tggaaacgat cgaacggttg agaaacgctg ggatcaatat
     1441 tggttccacg actggattta ctgctcctat gatgacaatc gtagcagaaa ccgcaaaaaa
     1501 agaagggtat tcaccagact tcatggcaac ttctgaagac gtcgaagggt atggtcgacc
     1561 gtttccttat atgatccata aaaacatgca gtacttcaaa aacaagtcgg ttgatgaagt
     1621 aatcaaagtc ggagatacgg tctcagatat ccaggaaggg aaaaatgccg gagtgttgac
     1681 agtaggtgta attgaaggta gttcgctaat ggggatttct tatgatgagt accaaaaatt
     1741 agagatgggg aaaaaagatc gtttaaaaca gtctgttgct cttagattcc aacaagctgg
     1801 cgctgatatt tgtgtaaaca atttaaaaga gttaacagaa tacattttgt acgctgacaa
     1861 gaatccagaa tgacttttat acaaaatcag aggtcgtgaa aaagctgtac tgcatcaagt
     1921 aataagaaca gccaaacaaa aatagctcct catatttttc gtttggttag gcttattacc
     1981 gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa agggagctgt gacacgactt
     2041 ttgtcacagt ccccttattt acaaacaaac agtgtttaag agacaacctc tccactaaac
     2101 ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac gataaaattt gaaaagctat
     2161 tttctctttt gtttctctaa tactcggatc agaacgctga tttcacattc tctttttctt
     2221 acatcttatc tggtgcatga agtcctagca aacgaaggtc ttctttcaat aatacagtca
     2281 ctgcataaac tagagctaag cgtgcttctt tttgttcatc atcagctaaa atcttcgtat
     2341 gagcatagta tttgttgaat gcttgtgcta atttgattgc atgtttcgca atcactgacg
     2401 gttcgtattt ttccccagca gacaagaccg tttcagggta tttttgaact aatttaacca
     2461 cttcccagct ggtatcgtca ttcaatgcat agtctgctgt ttctgaaggg acaaatcctg
     2521 ctttttccaa caggctgact gcacgagcat gtgtatattg cacataaggt ccagtttctc
     2581 cttcaaatcg aacaacttct tccaggttaa agtcaaatgt attcagtcga tcgtttttca
     2641 aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc tacagcatct ttgttttcca
     2701 aatcagggtt tttctcgctg atttgttctt tagcagattg gattgcttca tttaatactt
     2761 cttctaataa gacgatcttt cctttacgag ttgataattt tttaccacct tgcgtgatca
     2821 aaccaaatgg aatatggcgc atatcgtcag accagtcaaa gcccatttcc ttcaagacag
     2881 ctttcaactg tttaaaatga tagctttgtt cattccctac tacatataga gattgtttga
     2941 agtcgtaagt acgtttacgg tacaatgcag cagctaagtc acgtgtaatg tatagtgtcg
     3001 caccgtctga cttcttaatc aacgctggat tcaggtcata agcagatagg tcaacgatct
     3061 ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa aagtttgacg atttcatcca
     3121 tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa acgtacttcc agcagatcat
     3181 agattttatt gaattctttc atcgattcat cacggaacca ttgccataat tgaatagcct
     3241 cttcatcgcc ttcttccaga cgtttgaacc aagcacgagc ttcttcattt aattctggtt
     3301 ctgtttcagc tacttcatgg aattggacat ataaacgaag caattcattg attggttctg
     3361 ctttaacagc ttcttctgta ccccattttt tgtaagcgac gatcagtttt ccaaactgtg
     3421 ttccccagtc acctaaatgg ttgatgcgga ttggctgata accgattttt tccataatga
     3481 aaccgatcga attaccgata actgtcgaac gcaaatgtcc cattgagatt ggtttggcaa
     3541 tattaggaga agacatgtcg attggtactg ttccttgatt cccgatattg ctatccccat
     3601 aatgttcttt ttctttcact actgtttgca aaactttttt gctgataagt tctttattca
     3661 tgaaaaagtt caagtaaggt ccgacaactt cgattttttc aaagttggca gaatcgattt
     3721 tttctgctaa atctgcagcg atttgctgtg gcgcttttcg gtaaactttt gctaatgaaa
     3781 aggctggaaa tgcaacatct ccgtgttcag ctgattttgg attttctagt aattgttcga
     3841 cttgttccag tgttaaatca tctttgacag cgtcatagac agcttttgcg acaagatctt
     3901 tattattcat ctttattcct cctaaagtgc tcccttgatt gttaaacaaa aaaaatctct
     3961 agcgacggca tacttagtat gccatcacta gagacgagat ttcccgcggt accactccaa
     4021 ttgtcctaaa aaaggaccag ctccatctga tatcggtaga cgaccgtctg tttaaaacag
     4081 aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc ttccaccact tccgaattcg
     4141 ctagccaaga atgatacaag tactctcttg ttcaacaaat cttagtctta ttaaactttc
     4201 ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt ctgataaaaa aacaatccag
     4261 atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt aaatacgaaa
     4321 gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa aagattctcg
     4381 atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac aaatgcaata
     4441 gctgcctcta ctttcggaag catgcttcct ggagcaaatt gcttttcatc aatgtattgt
     4501 ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg gtaattgaca
     4561 tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat ttcggctaat
     4621 ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc agtagctgtt
     4681 tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg atcgaccagc
     4741 gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac tacttttctc
     4801 catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat ttgttctttg
     4861 gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa tgaaggatca
     4921 ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat ccctttttcc
     4981 ttcagtactt cgcccatggc gttttgtaac cagtaaccga tagatccttc tgtcattgca
     5041 acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc
     5101 aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc tccttgttcg
     5161 ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc ttttgcactt
     5221 gcatccgtag acaagatggc attgccccct aaagcgacaa ctacttttcg tttggccatt
     5281 tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag ccagcgtttt
     5341 gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta tgcttaaacg
     5401 cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga gtgcatacga
     5461 ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc tgttacttcc
     5521 atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg
     5581 aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt
     5641 tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt atcttcttca
     5701 cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc tttatcaaca
     5761 tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc cattttgacg
     5821 atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc tcctaaaatt
     5881 gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca gtaagcaaca
     5941 gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc gatcatttgt
     6001 gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa ttctgctagt
     6061 tcctctacca ttttttgact gaatccacgg aattcgattc cgtcaaacat acgtcctagt
     6121 actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt tgcacctaga
     6181 tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg agtacgtgta
     6241 gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg tgggatacca
     6301 cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa ttcttctttt
     6361 gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt catattaaac
     6421 attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg cagctgacag
     6481 taagactgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg ttggctcatg
     6541 cagcgtggac caccacgacc tcgtgacaat tcacttgaat tgatttctaa cactttgatg
     6601 ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac tacaacacct
     6661 ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc aacgatctca
     6721 tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc tttcatcgtt
     6781 tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc gattgtaaag
     6841 acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt atcgtgatct
     6901 accattgtaa atacggtatc caaatgcatc attgcacggt tgttcggaat tttgattgcc
     6961 aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt ttctaaagct
     7021 ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa gatcaattcg
     7081 tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat tcctttattt
     7141 gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg acgacgtgct
     7201 tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg gtcgcgtgta
     7261 aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc tgacagatca
     7321 tatagactgc tagaacgtac ttcgatatct tttgtacgaa ccccagccat gattttatct
     7381 accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct tacagcattc
     7441 gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac attgcctgcg
     7501 tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc attttgcaat
     7561 gtcttcgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat atcatcgaac
     7621 aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg ccgtttcagt
     7681 aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc catgttgtgt
     7741 tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca ctttcataat
     7801 tttgtctcat tcaaaatgag acattcctca cattaatatt cgataattaa ataattaaac
     7861 gtttttctat ttttaagcgt aatttattca ttttttattc tgtatacact ttcaaaaaaa
     7921 tatttattca ttgaaaaaat ttttttgaat aaattaaaaa aataaattat attatcgttg
     7981 ttagcgttta ctatatgtat tattgttgtt ttttatacac aaatcattcg cttcaaaaca
     8041 aattattcaa taccattgta tattttttac ataataacaa attatatttg taaagtatta
     8101 aaacttgttt ctaatgttgg tatgttaaaa ctttacaaat tctttaaaaa aagtgacaat
     8161 aaatatagtg aacaataagg agaggacttt ttaatgcgtg ttaataaaat cgattttcac
     8221 ttttcaaaac tcagatatca acctgatttt gaacatttct cggatgaaga gttccaacag
     8281 atcaaggaac atactgttct tcgttcttat aaaaaaggac agatcctttt cgacgatggt
     8341 gatcccagac aacggttgta tatcttgacc aaaggactcg ttcgtttaga acgttatgat
     8401 gaaagtgcaa cttattacta ctatgactat gtgaatacaa ataacctttt tcctatgtca
     8461 ggaatgtttg gagaagagac ctacacttat acagctcaag cgatgactga tattgaaaca
     8521 ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa aaaatcccga acagttgatc
     8581 tatatgatac aaaaaatttc taaagtagtt gaaatacatg aattgcgtat ccaaatcgga
     8641 ttgacctcta gcgcctttga ccgggtaaaa caaaatttat tcattttgaa agaagaattg
     8701 ggcgtaccaa caaaagatgg tcatatcacc attccttatc cgatcacctt aaaagaatta
     8761 gcagtgaata gcggcacgac acgtgaaact gctggacaag taatcaaaca actacgtgat
     8821 tccgggctgt tagattatga caaaaagcgt tttactttct gtaagacagc tactactcca
     8881 gctatgagag aataacgcga acaaaaataa gcagtgaccc tgataa
//
LOCUS       DADXTH010000095         8900 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.095, whole genome shotgun sequence.
ACCESSION   DADXTH010000095 DADXTH010000000
VERSION     DADXTH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.095"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1542
                     /locus_tag="IWI97_002429"
     CDS             <1..1542
                     /locus_tag="IWI97_002429"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBC2463905.1"
                     /translation="KMDASEYKNYLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSK
                     QTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFV
                     QLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAY
                     EFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNV
                     RNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYT
                     FDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAI
                     VLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDV
                     LFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIP
                     RYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEV
                     FKHEQ"
     gene            1532..2749
                     /locus_tag="IWI97_002430"
     CDS             1532..2749
                     /locus_tag="IWI97_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBC2463906.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2762..5911
                     /locus_tag="IWI97_002431"
     CDS             2762..5911
                     /locus_tag="IWI97_002431"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HBC2463907.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            6755..7006
                     /locus_tag="IWI97_002432"
                     /pseudo
     CDS             6755..7006
                     /locus_tag="IWI97_002432"
                     /inference="COORDINATES: protein motif:HMM:NF014026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
     gene            7064..7366
                     /locus_tag="IWI97_002433"
     CDS             7064..7366
                     /locus_tag="IWI97_002433"
                     /inference="COORDINATES: protein motif:HMM:NF025668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HBC2463908.1"
                     /translation="MTTTVSNKGSRDGYEVVQLYIGAQYGRYIRPTKDLVAYKKVFVK
                     SGDSVTVSFELTNKDFKYYDEEFNEIIDDGAYWIMVGNDSSQALTNKEEIEVRINE"
     gene            7437..8774
                     /locus_tag="IWI97_002434"
     CDS             7437..8774
                     /locus_tag="IWI97_002434"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014437.1,HMM:TIGR00826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2463909.1"
                     /translation="MTRLRFALRDDTKVDKAGLENLEDVKGTFNANGQFQVVIGPKVA
                     DIYEEVISQSGLSGANKEDVKKVAAELNLNWFQRAVKILGDVFVPILPAIVTAGLLMG
                     LNNLLTNPGIFFEQSLIEVYPNIADVADMINLIANTSFQFLPALITWSTVKYFKGNPL
                     LGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHWTIFGLSISKIGLQAQVLPALVSGYVL
                     AKTELFLKKRVPETIQLLVVAPVAILFTSVITFIIIAPITIAIGNTIVAGIVWLFQKV
                     PFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLMANGGDYIWPIGVMSSLAQGSAAIAMN
                     FLIQNKKLKGVGLAAGLSAYMGITEPAMFGVNLKYKFPFVVSILTSGIIGGFMASQHV
                     IATSVGISGIPGFLSIPFEFWPIFFICMLASCVVSFGATYLYGKNKVLLQSFK"
     gene            complement(8804..>8900)
                     /locus_tag="IWI97_002435"
     CDS             complement(8804..>8900)
                     /locus_tag="IWI97_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBC2463910.1"
                     /translation="ILGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         3000 a         1453 c         1837 g         2610 t
ORIGIN      
        1 aaaatggatg cgtcagaata taaaaattat ttattgggac tgattttcta taaatattta
       61 tccgatcgat tgttggaaca agtggttctt ttagcagatg aatccttaga ggaatatgac
      121 acagtaagca aacaaacaat gttgtatcgt gaattacttt cagatgaaga atcaaaagaa
      181 gatctgattg ccacaatcgt tgatattttg ggatatgcaa ttgcaccaga gtatcttttc
      241 aacgtcttag cggatcaagc aaaacaagca acgttccaat tgaacgatct aaacaaagcc
      301 tttgtgcaat tggcatctac ctacaatcaa tttaatgggt tatttgatga tgtggatctc
      361 caatcaaaaa aactaggaac agatgagcaa caacgaaatg tgacgattac cgaagtcatc
      421 aagaaattga atgatgtaga tgttttagaa cacgatgggg acgtgatcgg ggatgcgtat
      481 gagtttctga tcagtcaatt tgcttcagaa gcagggaaga aagccggaga attttacaca
      541 cctcatatgg tttctgatat gatggcgcaa atcgtaacgc tcgatcaaaa ggaacgacga
      601 ttctttagtg tttttgatcc aacgatgggt tcaggttcat tgatgttaaa cgtacgtaac
      661 tacttaacac acccagataa cgttaaatat catgggcaag aactaaatac aacgacatac
      721 aatctagcaa aaatgaactt gattttacat ggtgtcgacg ccgaagaaat gaatctccgc
      781 aatggggata cgttgaataa ggactggcca acagatgaac cttacacatt tgatgcagta
      841 gtaatgaacc caccttattc ggctaattgg tcagcagata cgactttctt agatgattct
      901 cggtttaatc gttatgggaa attagcgcca aaatccaaag cagattttgc gtttcttctt
      961 catgggttct atcatttgaa ggaaacaggt accatggcca tcgtcttacc acacggagtt
     1021 ttattccgtg gcgctgcaga aggggtcatc cgtcaaaaat tactagaaga tggcagtatt
     1081 tatgctgtga tcggtatgcc tgcgaacttg ttctttggga catcgattcc aacgacggtg
     1141 attgtgttga agaagaatcg tcaaactcga gatgtattat tcattgatgc tagtcgtgaa
     1201 tttgttaaag ggaaaaatca aaataaacta tcggaagaaa acattcaaaa aatcctcgaa
     1261 acctacgctg aaagaaaaga tgtcgagaaa tacgctcatt tggcaacctt tgatgaaatc
     1321 aaagaaaatg actataactt aaatatccca cgctatgtag atactttcga agaagaagaa
     1381 ccaattgata tggttcatgt cggaaatgac atcaagaaga tacgacaaga acagcaagtg
     1441 ctagaaaaaa aacttttaga agctatctct tccctgcaaa caacacctga aaatgaagcg
     1501 tggttacaag gtgcgctgga ggtgttcaaa catgagcaat gatacacaac cagaaatccg
     1561 tttcccagga tttacagatg attgggaaca gaggaagttg ggggacttcg ttgtagatta
     1621 tgttgaaaag acaagtgtac aaaatcaatt tcctatgcta acttcatctc aacaaaaagg
     1681 gatagttttg caagaagact attttgcaaa tcggcaagta actacagaaa ataatattgg
     1741 ttactttgta ctacctagag gatatttcac gttccgtagt cgaagtgata atgatgtgtt
     1801 tgtgtttaac cgtaatgaca ttattgatag agggattata agctattttt atcctgtatt
     1861 tactttaaaa tctgctgatt ccgacttctt cttaagacgt attaacaatg gaatccaacg
     1921 acaattatct attcaagcag agggaacagg tcaacatgtt ctgtctctaa aaaagtttaa
     1981 aaatatagta gcaatgtttc ctagtgagga agaacaacaa aaaatcggca ccttcttcaa
     2041 acaacttgac gacacgatcg ctcttcatca gcgtaagtta gatttgctga aggaaacgaa
     2101 aaaaggattc ttacaaaaaa tgttccctga aaacggagca aaagtgccag aaatccggtt
     2161 tcctggtttt acggaagatt gggaagagcg taagttggga gaattaggta aaactcaatc
     2221 gggaattgga tttcctgatg ccgagcaagg tggttctgaa ggtactccat tttttaaagt
     2281 ttccgacatg aataacattg gtaatgagta tgaaatgaga aatgcaaatc attatgtatc
     2341 taatgagcaa attgagcgta aaaaatggaa gccaataaaa gatgtgccag cgattatatt
     2401 tgctaaagtt ggcgcagcaa taatgcttaa tagaaaacgt ttggtcacaa gccctttctt
     2461 aatagataac aataccatgg cttatttatt taataatact tgggatatct attttggtaa
     2521 aatattgttt gaaactataa atttgccaag atactcccaa gtaggggcat tgccttctta
     2581 taacagttct gatattgaga atatcagtgt taaggttcct gtaaaggatg aacaacaaaa
     2641 aatcggtact ttcttcaaac agctagatga tacgatcgct cttcatcaac gtaagttaga
     2701 tttactaaaa gaaacgaaaa aaagcttctt acaaaagatg tttgtctagg agggaagaag
     2761 aatgaaaatt agtcaccgtg aggaagcaga agtcgaagaa cagttgattc gtgtacttgg
     2821 agaagggcat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag acctttgggt
     2881 caatttacgc caaaaaatta tctcaaataa tcaagcagaa ctgaatgatt ttcctttgac
     2941 cgataaagaa tttgaaacga tcaaaacgga attactgctg cgaacgaaaa ccccatttga
     3001 tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg aacgggaaga
     3061 ttcgcaattg ggttctgtct cacttatcct ttattccaat caagacattg gtggaggaat
     3121 ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag aagcacggga
     3181 tcgacgtttt gacgtcacct tattaatcaa tggattaccg attgtccaaa tcgagctgaa
     3241 acaagtaact gcaaaagatg gtgtttatca ggcatttaac caaatcaaaa aatatgcaga
     3301 agaaggtatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg tttccaatga
     3361 acaaaccact cggtattttg caaatgcctt accgaaagac ttgcacccta agtttttatt
     3421 tagttggcga acgaaagaca atgaaaaagt agaaaactta tatgagtttt gtaaacaagt
     3481 gttaaatatc ccggatgctc atcgtttgat tgcggattat acaatcgtca gtgaagatca
     3541 agacaataaa accctcatgg tgttacatcc ttatcaagta catgcgattc aagccttgtt
     3601 tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt caggaaagac
     3661 gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg atcgaacgat
     3721 catgttagtg gaccgcaaag atttagataa tcaaaccacc acagagttta caaaatttgc
     3781 ttcggaattt aatactggga tttcttccgg gaacgccaaa gcgaatagct tgatcgttgg
     3841 aacgggaagt gccaaagaat taagtgaaac cctgttggca gatgccaatg caaatgttgt
     3901 aatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga aacaagaaga
     3961 gaaaaaagga accaatcgct tccaaaaatt aatgggccaa catatcgtgt ttgtagtaga
     4021 tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa atcaaaaaaa tgtttccgaa
     4081 atccacttgg tttggcttta caggtacacc gatttttcct gagaatcgga aacaagccaa
     4141 agggcaattg gcacgtacaa cccatgatca gtatggagag gtcttgcata cctataccat
     4201 taagaatgcg ctagaagatg gctctgtctt aggatttcaa gtagagcatg aaaacaccgt
     4261 ggagccgact tcactagaga ataaaatcta tcggaaatta aaagaagtag aaacatatgc
     4321 agagtattca tcagaacaga tcaatcgtat gattgatcaa atggaacctg tcaaaaaaga
     4381 aagttatttg gacccagctg tttttgaagc agatgaacat atccaaaaag tcattcataa
     4441 aatgttccgt ccagataatg cctatacaaa atttgatttt cgaaatggca ggccaacgaa
     4501 atctgcaatc ttaaccacga gttccattga catggccaaa aagtattatc gggccatcaa
     4561 agagatgacg aaagagcctg attggttaac aaaagagttt cccaatcagc tcattcgtga
     4621 aggacgaacg atggaggatc ctgattttcc acgaatcgct attacctatt cattagaaga
     4681 aaatagccga gattcaagtg cccaacaaga agaaatgcaa aaaattattg aggaatataa
     4741 tcagtattat gatacttcct ggtcattagc agacattgag cgttataacg gagacatcaa
     4801 taatcgtttg gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa tcgatttagc
     4861 gattgtagtg gatcgtctat taacaggttt tgacgcccct acgattcaaa ccctatttgt
     4921 ggatcgtaat ctagagtatg caggattgat ccaagctttt tcacgtacga atcgtacgta
     4981 tcctgataag acaaaaggat tgattgtgac gtttcgtaag ccagcgacaa tggagaagaa
     5041 tgtcgaggat gcgacaaaac tgtattcaaa agcaaaagaa gaatcaggtt tgatttatcc
     5101 aagttatcag gaatccaaaa aaagattcaa acaagcatat ggtaagttaa aagaggtttc
     5161 atccatggaa tccattgatg aacatacgcc acttgaaatc cgtatcgagt acgtcaaagc
     5221 atttcaagaa ttgaatcgtg cttatgaatc gttagtgact tatgacgatt acaatgatga
     5281 catggtgaaa tctccaactt tgaacaatca aattcaactt ttggaagaac aaattggcgt
     5341 gtacgagacc gtgaagggtt cattaataga ggaagaacca gaaaaagaag gagaagattt
     5401 ttcaacgatc gaattttata gtgaaaatac cactaagttg tatgatattg attcaactta
     5461 tattgatcaa ttactaggaa cttatgctgc gaatagttca gatgcacgag aagaaattga
     5521 aaaagctttg gctaaattga ataaaacaga aggagtcaaa gaagtttacc gacaaatttt
     5581 aaatgcattt gatgtgggga ctttagatag taatgaagat atctttgtca ttaaacgtcg
     5641 ctattttaca caagcgagtg atgatttgat tcatgtcttt tccaatgagt ggtttgtttc
     5701 tgagaaagaa ttacatgctt ctaatctgca atatctacca ggtgaagatc caattcctaa
     5761 tatgaaagca attatcaaca gtaaggatta cgaaggatat aaagccaaac atccagaagc
     5821 gaagccattt aaatacccac aagaaatgaa gcgagcatgg cgtaaaatgt tagatgatga
     5881 gttgattcca cttgagaatg aattgaggta aacgagaaga gttaagaaaa ttgtacgatt
     5941 caaagatggt agtcaagtgg aagtgtagaa aatagggata gatgtctttt atttgttcac
     6001 attttataac gaagtattta agacagctat tgaataatgt ttcctccacc tttttcattc
     6061 atttacccca ccccgttgtt atatgaccgt gttttgagag gaaccaataa aaagcatagg
     6121 ctaactaggg gttccttttt cttttgtttt tttggaatcc gcttaagtga aatgtatctg
     6181 aaatgaaacg gagataaagt gattttttgc tataatgaca aggttttagc tgtttcagat
     6241 accatgtaac ccaaaatagt ttattgagaa aataggagag tatttgatac taactagtag
     6301 gaaaaaatga gataaaaatt aaatggaatc atttcaggta atctcaaata aaaaaatata
     6361 cagtcctgtg gcactttacc tatgggactg tatatttttt actctcagcg ggcttgtttt
     6421 gactccgtgt ctgactccgt cgtattgatt ttaaatgata ttcgatgacc atttaacagg
     6481 tcatttttga aatttttgtc tctatatgac tcagtttgat aattattgat agatcaattt
     6541 gggtctatat cgaacaaggg aataatccaa taaaaaatga tttagaatat caaaataaca
     6601 aaaaagagga ctacctgtat tcgtcctctt ttttgaccag attttgacca gtctaattta
     6661 cattctaata tattcatatg agctaatttt ttgattaaat ggtaagtttt tccttgtttg
     6721 ttttagttta aactagtgtg taatttaaaa atctatatgt aagggattag ttgaggcttg
     6781 gcatctagga gcagaggctg gcaatgctat tttggacgtc ctttgtggca aaataaaccc
     6841 agccggaaag cttacaataa caattccgag acatttagga caaatcccta tttattataa
     6901 ttatttcaat actggtagac cgtattcaag tgaaaagttt aatacgacca aatacttaga
     6961 tgtatccaat gaaccacaat ttccctttgg gtatgggttg agttatacca ctttttctat
     7021 gtcaccaatt agcattaaag agaagggtaa gaactgtttg aaggtgacta ctaccgtttc
     7081 aaataaagga agtagagatg gttatgaggt tgttcaacta tatattggag ctcaatatgg
     7141 tcgttatata agaccaacaa aagacttggt agcctacaag aaggtttttg ttaagtcagg
     7201 ggatagtgtg actgtttctt ttgaacttac aaacaaagat tttaagtatt atgatgaaga
     7261 gttcaacgaa attatagatg atggtgctta ttggatcatg gtaggcaatg attcatctca
     7321 agcattaact aataaagaag aaatagaggt aagaattaat gagtaataaa gaagaagcta
     7381 aatcaatcat taactttgtt ggtggaaaag agaatattgc agcactgact cactgtgtga
     7441 cacgtctgag gtttgccttg cgagatgaca ctaaagttga taaggcagga ttggaaaacc
     7501 tggaagatgt gaagggtacc ttcaatgcta atggacagtt tcaagttgtt atcggtccta
     7561 aagtagcaga tatctatgaa gaggttataa gccaatctgg gctgagtggg gcaaacaaag
     7621 aagacgttaa aaaagtagcg gctgagttga atttgaattg gtttcaacgt gcagttaaaa
     7681 tattgggtga tgtatttgtg ccgattttac cagcgatcgt tacagctggt ttactgatgg
     7741 gactgaataa tttattaaca aacccaggaa tcttttttga gcagtcactc attgaagtat
     7801 atcctaatat tgctgatgtc gctgatatga taaatcttat cgcaaacact tcctttcaat
     7861 ttctgcctgc gttaataaca tggtcaactg taaaatactt taaaggaaat cctcttcttg
     7921 ggattgtgtt aggtctagtc tttgtacacc cagatttgat gaatgcctgg aattacgggc
     7981 aagcagtaac cacgggagat attccacatt ggacaatctt tggattgtcc atctctaaga
     8041 taggactaca agcacaagta ttgccagctc tagtttccgg ctatgtgtta gctaaaacag
     8101 agctattctt gaaaaagcgt gtgcctgaaa caatccaact gttggttgtt gcaccagttg
     8161 caattctttt tactagtgtt ataacattta ttattatagc gccgattaca attgctattg
     8221 gtaatacgat tgtcgcaggt attgtatggt tattccaaaa agttccattt attggcggat
     8281 ttgcttatgg tattggtctt gcgccattag tcgtgacagg gatgcatcat gcgtttttac
     8341 cagttcatat tcaattgatg gcgaacggtg gagattatat ttggcctatt ggagtcatgt
     8401 caagtttggc acaaggttct gctgctattg caatgaattt cttaatccaa aataaaaagt
     8461 tgaaaggtgt tggactggct gccggtttgt ctgcttatat gggtatcact gagccagcaa
     8521 tgttcggggt taatctaaag tataaattcc cattcgtggt ttccattctg acctctggaa
     8581 taattggagg atttatggcg tcacaacacg tcattgcaac ttctgttggt atcagtggaa
     8641 ttcctggttt cttgtctatt ccgtttgaat tctggccaat attctttatt tgtatgctag
     8701 caagttgtgt cgtctcattt ggagcaactt acctttacgg gaaaaataag gttctgttgc
     8761 aaagttttaa ataaagaata aaatccctta cggtatctat gatttaagct gggattccca
     8821 ataatacctt gatttcagta cagaccgaaa acccgaagag agtgccttct tttcgggttt
     8881 tcttatataa tcctagaatg
//
LOCUS       DADXTH010000096         8725 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.096, whole genome shotgun sequence.
ACCESSION   DADXTH010000096 DADXTH010000000
VERSION     DADXTH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.096"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..548)
                     /locus_tag="IWI97_002436"
     CDS             complement(<1..548)
                     /locus_tag="IWI97_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2463911.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAA"
     gene            complement(587..1705)
                     /locus_tag="IWI97_002437"
     CDS             complement(587..1705)
                     /locus_tag="IWI97_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBC2463912.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(1702..3801)
                     /locus_tag="IWI97_002438"
     CDS             complement(1702..3801)
                     /locus_tag="IWI97_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBC2463913.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMTFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNH
                     QAFVTRLEELICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIYIQRRKR"
     gene            complement(3852..4673)
                     /locus_tag="IWI97_002439"
     CDS             complement(3852..4673)
                     /locus_tag="IWI97_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBC2463914.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(4670..5479)
                     /locus_tag="IWI97_002440"
     CDS             complement(4670..5479)
                     /locus_tag="IWI97_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2463915.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(5501..5995)
                     /locus_tag="IWI97_002441"
     CDS             complement(5501..5995)
                     /locus_tag="IWI97_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2463916.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6007..6495)
                     /locus_tag="IWI97_002442"
     CDS             complement(6007..6495)
                     /locus_tag="IWI97_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2463917.1"
                     /translation="MFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQEIKMTILDEVHVVNEEED
                     FF"
     gene            complement(6572..>8725)
                     /locus_tag="IWI97_002443"
     CDS             complement(6572..>8725)
                     /locus_tag="IWI97_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2463918.1"
                     /translation="PELLSATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLS
                     KENQEKLFVFIDTGNYRPVGENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVML
                     PTFEDRPIRERLELIQLFFQNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKI
                     SCADVYSRTREEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDY
                     DSILEKITQLINEGNFNEKFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSV
                     LGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPP
                     LSEKYENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEAL
                     DMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNL
                     TTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDK
                     LKDVFRHHLSDESIEVFTKEYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNIN
                     VYDILDGDGAANLKHILSSEMKPVEFNRLIQELIKFFSLEGIADRLNFLNPTIVMSEV
                     ETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKAEEPNYDMKPDEDEFYY
                     VSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         2637 a         1996 c         1450 g         2642 t
ORIGIN      
        1 gctgccatcc cctctaaaca atcttggtgg aagggacagt ttcctgaaaa attgtccttt
       61 ggatgtcgct tgatcaacat atgccccatt tccggatgac tgaacccttc cacgaaagtc
      121 ccattgaaca aggcaccacc tccgatacct gttcccacag tgaagtatgc aacacctgct
      181 aaccctttgc cagaaccagc aacatattca ccatagcaag ccgcattaac atctgttgtc
      241 cattcaatcg gaatattgaa atattgcttt agcgttccta caaaattgaa attttgccaa
      301 gcaagttttg gtgttgatgt gatatgaccg aaactcgttg atttgcattg aatatcaatt
      361 ggaccaaagc agccgacccc gatgcctgcc aattgttcct taaaaggttt aaaaaaatca
      421 atgacctgct tcattgtttc aactggagtt gttgtgggaa aactgactct ttccaaaatc
      481 gttaattcct cagtaccgac tgcacagaca aatttagtcc ctccagcttc aattgcccca
      541 tatatcatca taggttcctc ccttcgaata tgtgaaaaac acttcattaa cgcggcaagc
      601 gttctcgatc agtgaaatga cgtttagtat ttcctgaagc ttctgttgaa taccagtaag
      661 cgacactagc aacatcgtct tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt
      721 gtaaagtgac cgataaatct tcggtaaaac agatggggtc ttgaagatgc caacgataaa
      781 gaccatgtcg tggcattgcg tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt
      841 caaaacgatc tcttgtggca tctctatttg attggtaagg ataccctaaa aaggctgttg
      901 aatagttttt cgcatgtgga cgtccgaact catcgtactc atgaaaggcc cacgccccgc
      961 caaaataatc ttctgaacct gttgaggtta cggtcggata ctcactatcc ccatcaatgt
     1021 agaatttaaa ctcgccttcg ccccaccaat aacgttctag gcaggtaagc gcaaggtaag
     1081 tacccacgta atagccttga ccctttaatc gatcaattaa cttataatcc tcctgtaatt
     1141 tggtaatgcg ttcccgatgc caatttgcgt gaaaatatag gctattttct tccaattcgt
     1201 ccaccaaagc gtaatttatc gtataaaaaa atgaggacat atccttcgga tgtaaattct
     1261 cgatcgtaat tttagcggat gtttggaaag gcatttcgaa ataggaatta aagccccccg
     1321 taggattaac cactataggc aacgagttca cctcgcaacg ctctccaaac ccattacaaa
     1381 agaaatcccc taaaggactt tcaacagccg ggctatccga cccatcccaa taaattctta
     1441 agattaaatc acgtaaaaca aaactgccaa attctgttcg atcacgaatc gtcatccaca
     1501 tatgacgaat tattcccgtt ccagtaatat ccgcaatcac agtttcttca ccagaagtca
     1561 gcggaattgc gccacgacct ttgcggcttg gtcctagctc gcttggcaac atcgcagctt
     1621 tacctttctc gcctgttgga ttttcgggag tgattgctcg actctggatt tttttaaatt
     1681 gggtaatatt ttttagtata ctcatctttt tctcctttga atgtaaatcg tttttccctt
     1741 ttctacgtga agaatttttt tcgtatcact tattttcaat ggcttttctg acaaatttga
     1801 aatactcaac tcggattgcg tacattcaat cgaaatgaag gcttgaccga acttcaaatt
     1861 tttccaagaa aacgttaaat tatcaacagg atcaatttgc aatactcctt ttttgaattt
     1921 caatccgaaa tattgactga tcattatact gacaaaaagg cccgatgccc agccgcattt
     1981 gccgcagcca aagtctcgca caacttctcc ttgtttagcc tctaaacgat acggccacca
     2041 ccaccatgaa ccgtctaaat ctgccaaaca aatcagctcc tctaatcggg ttacgaaggc
     2101 ttgatggttg tgcatgtcac tcattaaaac agtgataaag ccaggaaaag tggcgccgga
     2161 ttctaatccc caagtgatgc ctttgatttc ctcgctataa gttggattga aagaactagc
     2221 agaaaaggtc atggtgttct gatataaatg atctttatta tctaaaaatt gataaaaagg
     2281 catcaacgta gtgtctgatt cttctccatc atgcatcaat aaattaattt ttccatccac
     2341 aatcacatca cttaaaaaga ttaacccttg ctcaagaatc ggtttttgat agtgcttcac
     2401 tgaataggtt gttttctcct catcaccgat tccctccaaa aattgctcac caaaaggacc
     2461 atcgatagtc atttgtttac aaattgattc ctttagttta caggcaatgt ttttgtatcg
     2521 cttgctaaga ctcagttgtt ctagggcttc aaaaatttct gcaagtccaa tacaggcctt
     2581 ccaaagacaa agattgctgc cggtatgata ctttccaagt gcaaaggcgt ctgataacca
     2641 aacagatgga aaaagcattg gttcccctga ccgacgttga gctaatatgt cctcaataat
     2701 tcttgaagca ttcagtagaa cctgcggaca ccgctcaaag aattttgtat ccccagtatg
     2761 ttcaaaataa agactagcca gtaacgcact ggataacgaa ttactcagag aatgattgat
     2821 acctcctgta aattttgaac caggaaactt aacgccatat tgatcaaacc atagaaccgt
     2881 tttttgacat aactccggtt caaacatggt gaatggcaat gatgcgtaat acatatcctt
     2941 attccaaatt cgattagtag caggatagct gccccaattg ctgccgacga cctgtccctg
     3001 tcgattcata ccgaaactag aaaagctttg ataaagtgca cgacgataga gatggacaac
     3061 tctatcgttt ggcattgaca gctgcccaaa tagctgagca aaatagttca atgtttcctt
     3121 cagccatttt tcgatatcag attcctgaaa agtcattact tcttcaaaag catttgggtc
     3181 cacaaaagca atggcgaagc ttgccacttc ctgttgtgcg atcgtgtcct tatttccttt
     3241 ttgagaaatc aacacatttt gctgatccga atacttctct tggtacaaag gcacatcttt
     3301 cactgaaact tgaaggcttt cgtgtgtttc attcacaatg taaacctgtt gaacaagcat
     3361 acttaaacgc ttattagcca gtattggagc aaaggatact acaatgacct taaacgtcga
     3421 ataatgatga atatatctag gcaaacagtc ttgaatcaca tcgacttcaa tacgatgatc
     3481 cgattctgac aaatggtaga caatcccgtt tacttcaacc tttaaggata acgaattggt
     3541 ttgagaaagc tgtttattga cccaaacacc tggtttttcc tcagttagta aattttctcg
     3601 ataaaaggtc aacgtttgaa tcgtcccgca aacatcaagg tttcctaatg tcttttgatt
     3661 acttacatcg aaggtcaatc cctttacatc aggaatattc cgatagtagc tgtattcgtt
     3721 tgaagtagtc acatttccgt cgtttaactc agcattaaat aatgggtgtt tgggtgaata
     3781 aattacttca ttaatagtca tcttttccct ccttgctaaa aaaactgcag cctagtagct
     3841 gcagcttcct actatgcgat tccaagtaat gacaataaaa ttcccaagac gaccattcca
     3901 aaaataattt tgatgacgcc gactttttta tctagtaatt tgaaacagcc caatgttacc
     3961 aataacggta caattccttt aaaaatacta tcgaggattt cttggacatt cattagttct
     4021 tcgcccttca ttgaaaagct caaaacggta ttgaatttga ccatactgat cgtcattgcg
     4081 cctaccatga tcagacccaa gatactagca gcttttgtca gaatttcaat taacccgttc
     4141 gcatacagct tttcaatata tttagagcct aatgaatagc ctaaaacgcc gccatagtat
     4201 cgcatcaaat aattaggtat attgaacata actaaaaaga taattggtgc caaaatattt
     4261 ccggcattgg ctaagttgat cgctagtccc gcggtaacga cacgccaaac tccccagaac
     4321 aatgaatcgc caattccagc caatggtccc atcaaactcg ttttgatcgc gttgatactt
     4381 tccacgtcaa aaccagcttt ttcagaattt tccttctcca tcgatgccgc aatccccatg
     4441 acaaaggacg cgcaaggaat ggtcgtcgaa aaatactgtg tatgacgttc cagcgccgca
     4501 gctcgatctt cttcactttt gtagaaatga ttaatgaagg gcatcataga ataggcgaaa
     4561 ccaccgccgc ccatcgtcac cggattcacc gaagcataca tagtcatcga gcgccaaaac
     4621 atcttccgaa ccaatttttt ctcatcttta gaaattccat cagctaattt cattgaaaga
     4681 aatcctcctc ttcttggtca agcgttgcat ttacagcagc atcgctttga actgcatgaa
     4741 cagatatatt ttttagttgt agatctcttt gggctataac aattgcaata atcgttccaa
     4801 ctgcggcaat tgcaatcaat ggtaactcga gatacgccat aacaacggcc ccaaagaaaa
     4861 accacacagc aatattttta ctccaaagca tccgtaacag catagccatc ccaactgccg
     4921 gcagcagatt tccagcgact gttaatccgt tcatgatgac ctctggaata ttatccaaga
     4981 cgcttttgac aacatctgct ccaaataaaa cggcaaagaa tggaataaga gcataaagcg
     5041 catagtggac aaaccacata acgaagtgca gattgatgat tcctttttga tcaccagatt
     5101 ttgcaaattt gaaaaattta gcagccatcg gtccacatac aaaagaaaaa atcatattca
     5161 ttaactgtaa tcctaatact gctagtggaa agacaatggg aataatcgca tccacgtttc
     5221 ctcccatttt gattgcgaac gctgtagcca acccagcagc caaagaaggc tccgccgtac
     5281 tagagattcc gatattgacg acacccataa aaatggcttc tagtgacgcc ccgacaatca
     5341 accccgtctg caaatccccc atcaacgctc ctgctaacgg agcaacaaca atcggacgac
     5401 tcagcatgga catcccgaac atttcatgtc cagcaactcc aataaaaacg gccaaagcga
     5461 ctacaaatgc atcatacata atttttctcc ttcctttttt ctaaaaatca ctcaaaatag
     5521 agatagctga acgtttttcc atcgccggtg taggctgaaa aacggtatct ggataaagtt
     5581 caactaattc ttttaagtct tgaatttctg acgtcgttaa catcacttcc tttgataagg
     5641 ttttcttatc ttccgatggc ggtgtcattt tcccagcatt ccccacgttc attaattcga
     5701 tttccgttgg gaaagctttc gcaactttta ccgcatcttt gacattaggg actaaaacca
     5761 tcaagcgcat gtccttcgcc cgcggatctt ttaacaaatg aatcgcgtca tcaactgttt
     5821 taataatgac tttgacattc gatggtgcag ccatcttcaa tgtcatcttt tgaatatcgt
     5881 tggtagccgc ctcatcgctg gctactacta acccggttaa atttagactc tttgtccaag
     5941 tcatcgctac ttgtccatga attaatcgtt catcaattcg cactgcttta atcatttttc
     6001 ttcctcctaa aaaaaatctt cctcttcatt tactacatgt acctcatcaa gaatggtcat
     6061 ctttatttcc tgttggcatt tatttaccag ttcctgaata tcctcctttg ttaattcctt
     6121 ggttgccaat actatttcga gcagaattgg cagattaaag ccagacaaaa tagttacagc
     6181 aatctctttc tccaaaaagg ttttggtaat cttctgattg acacttccgc caaataaatc
     6241 ggtaaaaaca aaaatctgat cctgctcgtt cacctggttt acaaatacat tcaactcctg
     6301 ttcataatca cactcatcca cataagcatt aatcaccgta atctcttgaa ccttccccgt
     6361 cagtaattga atactgctaa gaaagcctga agctaagtga ccgtgagtcg ctattagaat
     6421 tttcttcata tggttaattc ctttctttct gttcactttc atataagcaa ttcctgtgcc
     6481 aacttttaaa aacattggtt taatcacgtt aattcacata aaaaaaagtg tctaacctga
     6541 aaaaatcaga ttagacactt ttttagacac tttatttttg aatatacggt cctaataatt
     6601 catagagcag cgaaagctca taagcattga ctttaaaatt atatttcatc tccattgatt
     6661 gaaaaatact tctcgaaaca taataaaatt catcttcatc tggtttcata tcataatttg
     6721 gttcttctgc tttttctgtc cgcgccaaga ataaacgttc catcatcaat gccgtatgca
     6781 tataaagatt tagttttacg cggccattta aagtgaaatg atagtaattt tcatatttag
     6841 aaatgactgt ttccacctca ctcatcacga ttgttgggtt cagaaagttc aaacggtctg
     6901 cgattccttc caatgaaaag aattttatca gctcctgaat caaacggtta aattctaccg
     6961 gcttcatctc agaactcaaa atatgcttca aattggctgc tccatcacca tctaaaatat
     7021 catatacatt gatattagga attgaaaagg aacgtggaag atccgaagtc gtgatgacca
     7081 attttgtctt gtcaaaataa gtttcatcat tttggtcgat taattcacgt agttctctgt
     7141 attccttcgt gaaaacttcg atggactcat ccgataaatg atgacgaaaa acatccttca
     7201 gtttatccga gatacccaat cccgacatgc aggaaatgat gatgttgcta ctttgagaaa
     7261 agccttcaaa gtattgaaca ttgattgaat acttgtctgc tgcatcctcc gcaatctgtt
     7321 taaatggcgc attcatcgtc atcttcaggc caatatcaag tgcaatggag gtcgtcaaat
     7381 tattcagaag aagtaattct ccttttaaat cgttcttgat ctgtgtgtag atttgattta
     7441 aagagcccat atctacgatc agcactagac tatctgtatt cacttgccgt ttcacgaaat
     7501 tccttgtcga ttcgacaatc tctgacacat tactctctac tggcatatcc aacgcttcaa
     7561 aaacataatt gccgcataat tgattcacta ctgcttgaat gctgctagcc gtactatctc
     7621 cgtgagcaag caacaggccc ttcagctcga tggattcatc tacataatca gataaagtca
     7681 cgacaataag cagtcttaga aagttctcgt acttttctga gagcggtggt aaatgctcca
     7741 agaactgatt acataagtaa gtcgtccgag gtagatttgc agctaatgta cgatttatcc
     7801 cggttaagtc tgtcttatag cttggggaaa tgacatagga tctgctaata atttctgcaa
     7861 caggtccact cgtcgtcaat ccgtacgttt ttcctaaaac ggatacccag acctttctaa
     7921 acatctgaat catcaatttt ctactcattg ataaaaaaga atccttatca atgtgatgca
     7981 ccaaatgctg tacttcattc tttatcgaag aaaacttttc attgaaatta ccttcattta
     8041 tcagctgagt aattttttcc aaaatcgaat cataatcaaa cggtttgatt cgatccgttt
     8101 ttatcttttg atttttcttt atcaataaac tagcaatcgg caagctttct acttcggatt
     8161 ccagcatatt taactgaatc aagatctcat ttttcccaat cgttaatggc tcttcccgtg
     8221 ttcgactgta cacatccgca caactgattt taatgatatt cttcagctta ccaatattcc
     8281 cactaaaggg atgagataac attagagaca aagcttcttt tgaaacaata atatcttttt
     8341 gtaaaatatc cgcttcattt tgaaaaaata attgaatcag ctctaaccgt tctcgaatgg
     8401 gacgatcttc aaaagttggc aacataacac tgatttgaat acgtcgatca aaggtgtcta
     8461 ataaataatc ttcacttttt tccgtcgtgg cgaagataaa acgaacctta gcagaatgcc
     8521 agtttacatt ttcaccgact ggacgataat ttccagtatc aataaaaaca aagagctttt
     8581 cttgattttc tttcgacaaa cgatggacct catcaagaaa aagatagcca ttgttggctg
     8641 ttgccaataa cccctctgta tccttctcag cccccgtaaa ggcccctttc ttgtatccaa
     8701 acagtgttgc cgaaagcagt tcggg
//
LOCUS       DADXTH010000097         8426 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.099, whole genome shotgun sequence.
ACCESSION   DADXTH010000097 DADXTH010000000
VERSION     DADXTH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.099"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(30..731)
                     /locus_tag="IWI97_002444"
     CDS             complement(30..731)
                     /locus_tag="IWI97_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBC2463919.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(747..1187)
                     /locus_tag="IWI97_002445"
     CDS             complement(747..1187)
                     /locus_tag="IWI97_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HBC2463920.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(1187..1762)
                     /locus_tag="IWI97_002446"
     CDS             complement(1187..1762)
                     /locus_tag="IWI97_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBC2463921.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(2173..2652)
                     /locus_tag="IWI97_002447"
     CDS             complement(2173..2652)
                     /locus_tag="IWI97_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HBC2463922.1"
                     /translation="MFLDSGVVLKETELERIEYTDFGLGAIETEGLNLFVYENNERYC
                     GKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKLSCIIPKGQE
                     RYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDSFTNPAIYR"
     gene            complement(2690..3169)
                     /locus_tag="IWI97_002448"
                     /pseudo
     CDS             complement(2690..3169)
                     /locus_tag="IWI97_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(3341..4042)
                     /locus_tag="IWI97_002449"
     CDS             complement(3341..4042)
                     /locus_tag="IWI97_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2463923.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(4150..5592)
                     /locus_tag="IWI97_002450"
     CDS             complement(4150..5592)
                     /locus_tag="IWI97_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2463924.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(5606..7471)
                     /locus_tag="IWI97_002451"
     CDS             complement(5606..7471)
                     /locus_tag="IWI97_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2463925.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIETIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(7592..>8426)
                     /locus_tag="IWI97_002452"
     CDS             complement(7592..>8426)
                     /locus_tag="IWI97_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2463926.1"
                     /translation="LNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAVQKEFVL
                     RDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLHYAITRA
                     KENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHFVNSQQG
                     NEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLKGEGSDE
                     QDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVSEKKSIQ
                     S"
BASE COUNT         2408 a         1881 c         1390 g         2747 t
ORIGIN      
        1 tttctttccc gtcgtttggt tttttacttt tagcctaatt cttgaccttt cgcatgctga
       61 ttatctgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
      121 tgacgaatca ccttttttgc atcttctgga ttttctacaa aatcgaatgt atccccatcc
      181 acttgtattt taggacatac atcaaaatcc tcataccatt gattatagcg ggtgttcagt
      241 gtttcgtaat aggtgtataa cgtttcatcg ctttctagct gttcatagtc acgcccgcgt
      301 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcagga
      361 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
      421 tgctgtactt cgatatccgt tacccgacct aaatcagcat ttagatggaa caacagacta
      481 tcttcgtaga tggagcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
      541 aaaaatcgct tattcaaaaa gaagatttgc aataaaaatg cgtactgctc aggatttgaa
      601 taaaaaagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
      661 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
      721 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
      781 aatttttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt gtgaagactt
      841 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta cgatgatcat cggttttttc
      901 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
      961 tcaaggatag ccaaaacaaa ttctgcattt gtcaactctt ccatatcttt ttcgtatact
     1021 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
     1081 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
     1141 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
     1201 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
     1261 tgttggaaaa gatcacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
     1321 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
     1381 tatcaggtgt caggccgatc gctatccaag aaagaagttg cgaaaattcc tccaatgtac
     1441 ctagacctcc agggaaaacg aggacagcat ctcctttttc aaataataat acctttcttt
     1501 catcaattgt ctctgtagga ataaatgtag taacatcttt cccaggcagc tcagtttcaa
     1561 aaaggccatt tggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
     1621 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
     1681 tatattttcc taacgtttgc gcaataaatt tgtaactttc tttattgcca aaacgggaac
     1741 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
     1801 tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg tatatagatc
     1861 aaacaccact atatatagta tttgcaaaaa aataattttt cagctatttt acagtaacca
     1921 aaaaaataaa taactagtaa atataaaatt gaaacgactt acttatagaa gaaattggga
     1981 tttcgtcctg catgtcaata acacgcatta tttaaatagt cacacacttg tttctcctat
     2041 taatgaaaaa tccattttat tctcgtgtta tgtttcacca acgcctgatg tttctctaaa
     2101 taaaaaagcc gacgaaccaa ataatcgaaa tttgatcatt ttggttcatc agcttgttcc
     2161 caaacctttg tattaacgat aaatagctgg attcgtaaaa ctatcacttg gatcatcact
     2221 gctggaagaa aattcggtga taaccgcgcc tgttgcacct gcctggaacc agtgtttaat
     2281 attttgttcg atcgtgtact gatctccagc gtgaagtatg atttcttttt ttgccgtata
     2341 gtaacgttct tgcccctttg gaataataca ggaaagtttt tttgtttctt ttccttcgac
     2401 ataaagatat actaagcctt ttcgacaacg aaaggtttcc ttttttcctt cattctcatc
     2461 tcgaggtggg tgccgatgct ctggacaagt ttgataaggc aataatacca gttctttccc
     2521 acagtatcgc tcattatttt cataaacaaa taaattcaat ccttcagtct cgattgctcc
     2581 taatccaaaa tctgtatatt cgattctttc taattctgtt tctttcaata ctactcctga
     2641 atctaggaac atttgttgta ctcttttctt gtaatccatt ctgttcctcc taactgattg
     2701 tcaagtcatc ccaagatatc tcttcttcct ctttttcttc ttggatgact tctgcatttg
     2761 ggtcaactgg ttttttcaat aataaaatag ttattcccgt agcaaaagaa ccagctaaca
     2821 atgcaccaat gaatgcccat ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg
     2881 gaatacggct atctgcgccc caaaacatgc tcaatccacc gccgactgcc ccaccgactc
     2941 ccgttgctat gacattgcgt gccaagtcgt tcaatacgat agggaaacat ccctctgtga
     3001 tctggcagat ccccatagga aaagcggtct tcaatgtatc gatttcttga cgattgaata
     3061 tatttttacg aatgattttc cctaagaaat aagcgatcgt caagccgaat ggggttacca
     3121 tcgaagctaa aatcaataca gtgattggtt catagattcc ttctgaaaat cgtgccacac
     3181 ccccttatcc agaataaatg gtaatccaga aacagccggg tatctgcggt aataagccta
     3241 accaaacgaa aaatatgaga agctaatatg agaagctatt tttgtttggc tgttcttatt
     3301 acttgatgca gggcagcttt ttcacaacct ctttttttat ttatgtttca taatcaatat
     3361 ggtaaggatg cagctccgta tggtagaccg cctccgaata ttcaatcatt tccccaattt
     3421 gcgtagacga tttccgtatc cgtttgatgc cgatctgtgc gtctgttttc agaatttctt
     3481 gttcttcttc tgttaaaaat actgccgaga aatcatcaga aaatgagtca atagtatatc
     3541 cttttttcgt cagcaaacga tacaaagatt ctttgccaaa ttcttctctg ctgaattgca
     3601 tcatttcttg tggaagataa tgaacaaaat aaatataagg ctgattgttc aaataatata
     3661 agcgcttaaa acaaacagct tgctcaccga aaaaggaaaa caaaggatct tccttcttta
     3721 tatggaccaa ttgcaaatcc agcgtttctt tccggacttc atatcgggag ttctccaaga
     3781 tttgcgtaaa gcttgccgct ttggataatc gattgtaagg acgattgctt agaaccgtcg
     3841 tcccacgacc acttttcttt gcgacatatt catctgatgc cagcagttcg attgcctttc
     3901 ggacagtgat tttgctgact tgaaacagtt cttctaattc actttctgtc ggtaacatcg
     3961 aaccgactgg atattttcct gagaaaatat cttctttaat tttatctgcg acttccaagt
     4021 atagtacacg tgtccttttc ataacgtcac cctttgcttt actattacta aaagaatagc
     4081 acataaaaaa taaaaaaaga acacaaatca catgtgagaa gtgtgttctt tttcattcag
     4141 ctgctttaat tacaagttta cgccatttga ggcaattact tctttatacc aatcaaaaga
     4201 ttttttcttt gatcgattca gtgtcccatt tccttcatcg tctagatcca catagataaa
     4261 gccgtaacgt ttactcattt ctcctgttga tgcactgaca agatccatac atccccaagg
     4321 cgtatagccc atcaaatcta caccgtctgc tactgcatta gccatcgctt cgatatgacg
     4381 acgtaaataa tcgatccgat aatcatcaat aatcgtgcca tcctcttcca tatgatcaat
     4441 ggctccaaga ccgttttcaa caatgaataa tggtttctgg taacgattgt ataattcatt
     4501 caatgcgatt ctcagccctt ctggatcgat ctgccagccc cattcacttg cctcaagaaa
     4561 cggatttttt actcctccca gcaagttccc ttctgaagtt gctgcttgtg gatcagtctc
     4621 attgattgcc gtagacatat aataactaaa tccgatgtag tccactggat attcttttag
     4681 caactggaag tctttttccg tttgctctaa tttttccgga tcgactccat attttttatg
     4741 ggtacgcgct gtgtatgcag ggtattctcc acgtacttgg acatccgtgc agaaaaaatt
     4801 gaattcttgg ttttggatca ttgttgctgc ctgattgact ggattggcat cgatgctgta
     4861 agttgttgca tagataatca tgcagccgat ttggatatcc gaacgcaatt cgtggccgat
     4921 ttttacggct ttgctacttg caacgaattg attatgccat gcttggaaaa tattttggta
     4981 ctctcctgct ccattgcttt ttactaatcc ttgacttaaa gctggaaagt gaaaggccga
     5041 attgatttca ttgaaagtca tccaatattt gactttactg ctgtatctct ctaatactgt
     5101 ctgagcaaat cgttcataga agtcgatcag ttttcgattt ttccagccac cgtattcttt
     5161 tgccaaatga agaggcattt cgtaatgaga aatcgtgatc acaggttcaa tatcatattt
     5221 taagcactca tcaatcagct gatcataaaa ttctaatccc gcttcgttag gagtaccttc
     5281 gtctccattt ggaaaaattc gtgtccaggc aatcgaaaaa cgataacatt tgaatcccat
     5341 ttccgcaaac aaggcaagat cttctttgaa acgatcgtaa tgatcaattc ctcgatgatt
     5401 cgggtaaata tatttttctt gatcaatcgt ccagtcaaat tcttcactgc cgataatcgc
     5461 aaagcgctgc ttacctccag gcatagcatc tgcaacggat agcccttttc cgtctcgatc
     5521 ataagcccct tctaattgat tggcagcagt tgctccaccc cataaaaatt tttctgaaaa
     5581 acgtgattgc atcctgattt cctccttatt ttacaattgc cagaacatct tcgtttgcaa
     5641 taatctcttt ttggtctaat tcaagtatat cggcaaatgc atcagtattc gtaatgacaa
     5701 ctggagtgat tacgctatag ccagctgctc taattgtttc gatgtcaaaa cgaatcaata
     5761 aatctcctgc tttgacgaga tccccttgtt tgactaagat ctcaaagcca ttcccattta
     5821 attctactgt atccatcccg atatgaatca aaatctccgc tccgtcagtc gttgtgatcc
     5881 ctacagcatg tccagttgga aaaatagtcg tgatctctcc gtctgcaggt gcaactagtt
     5941 ctcctacttc tggatcaata gcgatacctt ttcccatcac gccagaagaa aaaacttggt
     6001 ctggtacttc actcaatggc agtactttac ccgttaaagg actttttaag gtatgccgtg
     6061 ctgaagtaat cggttctcct gcattcgcat gtttgttttc taattgattg tcttgtgttt
     6121 gttcgtcaaa tcctaaaatc aatgtaccta cgaatgcaag aacaaaagca attcctgtag
     6181 ctattactgc tactgtcaca tttgactcta caccatccac cgtattaatg aaagtcggaa
     6241 tcgtcaataa gctgaccaaa ctgctggaga aagcttttac tccggaaaat ccgataatag
     6301 ctccaccaat acctccagaa atacaagcgg caatgaatgg tttcttcaaa ggtaatgtca
     6361 caccatacac agttggttca gtgattccaa ataacgaagt cactgtagaa gaaatcccta
     6421 atgctcgaag tttcgtatct tttgttcgca atgctacagc taaagcagcc ccaccttgtg
     6481 ccaagatggc tggaagaagc attggcatca atacatcaaa cccatattgt tcgatattca
     6541 aaaacatgat cggtacgaat ccccaatgca tgccgaacat cacgaatact tgccataaac
     6601 ttcccatgat tagacctgct acgattggac taaatccata aatcgagttg aagagaccgc
     6661 ctaaagcatt accggcaacc gtcccgattg gtcctaaagc aagaaatgtt aatggaacca
     6721 tcactactaa aacgacaagc ggaactaaaa tcatttgcag aaattgcgga atcacttttt
     6781 ttacgaatgg ctctaattta ctttgaaccc atacggctag aatgattggt atgacagaag
     6841 aagtataacc agatggtgaa agaatcactg ggatcccaaa gaaactgata gcttctcccg
     6901 caccagcaac tgctgtgata gctggataca caagtgccat agcaattgct actgctaaaa
     6961 actggttggc tttgaacttt ttagctgctg taaaagcaag catgacaggt aagaaggtaa
     7021 agacaccatc tgctgcagca aacaatattt gataagtacc gctatcagct gtcagccagc
     7081 ccatcaccgt tgccaatgat aagaatcctt tcaatacccc ggcggctgcc atcgccccta
     7141 gaaaaggtgt aaatatacta gagataatat cgatcagacg attcaaaata tttttggact
     7201 cttcttttcc atcctccgaa actgcaagac cttcctcatc cacgatcgat tgataaacat
     7261 ctgctacatt actgccaatc actacttgat actgaccgcc actttgtacg acttggatca
     7321 cgtccgggtc ttcttttaac ttttgcgtat ctgctaaaga ttcatctttt aatctgaatc
     7381 gcaaccgcgt agcacaatgt actaaacttt tgacattctt ttgtcctcct accgcttccc
     7441 atacacgacg accaactgct tgattatcca ttgttttttc cttctttctt ttttatttaa
     7501 aaaataaaaa cccagattga aaaaaacagt cacaaacatt cgtgaaccta ttttttcaat
     7561 ccaggttttg cctgccatac agtaacaatc cttatgattg gatgcttttt ttctcactta
     7621 ctactctttg aatatgaatc gttaaataaa tctgctctgc gatccccatt tcctgttttt
     7681 gcgtttgtcg aagatactca ttgatccgtt cgacacaatg aaacgctttt ggatactttc
     7741 cttgaactaa tgcgtagaga aattcatctt gctcgtcaga tccttcccct ttgagataac
     7801 gctgggcaaa aaactgcaga tgagtaatga tacgctgata attcaaagat tcttcatcga
     7861 acacgatgcc gaagaattta ctgatgatac tcagtatatc tcttaccatt gtggtagcgg
     7921 tcatcgtcac ttgcatgtct tcgtttcctt gttggctatt cacaaaatga aaggcaataa
     7981 aaccggcttc atcctcagaa agttccacac ccgtcttttg tttcacgaac aaaagagcct
     8041 ctttcgctgc ttgatattct ttcggataaa atttttttgt ttcaaataat aatggattag
     8101 gcagttggat attttctttt gctcgtgtga tcgcataatg aagatgatcg gtcaatgtca
     8161 gataaatatt aggcgaaaat tcaactttta gtttatcttc agccagttcg actacttgtt
     8221 tgacccattc gatctcctct gccggaatat cagccatcag ctgctggaat tggtttttag
     8281 caaaagaatc tcttaaaaca aattcttttt ggaccgcttc tggcgaaatc tcgtccccaa
     8341 tctttttttg aaaagctagt ccttttccca tacaaacgac ttcttccccg aatccatttt
     8401 tcgaaatgac gacattgtta ttcaaa
//
LOCUS       DADXTH010000098         8402 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.100, whole genome shotgun sequence.
ACCESSION   DADXTH010000098 DADXTH010000000
VERSION     DADXTH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.100"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(36..533)
                     /locus_tag="IWI97_002453"
     CDS             complement(36..533)
                     /locus_tag="IWI97_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBC2463927.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(663..1373)
                     /gene="budA"
                     /locus_tag="IWI97_002454"
     CDS             complement(663..1373)
                     /gene="budA"
                     /locus_tag="IWI97_002454"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBC2463928.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1386..3050)
                     /gene="alsS"
                     /locus_tag="IWI97_002455"
     CDS             complement(1386..3050)
                     /gene="alsS"
                     /locus_tag="IWI97_002455"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBC2463929.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3255..3917)
                     /locus_tag="IWI97_002456"
     CDS             complement(3255..3917)
                     /locus_tag="IWI97_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBC2463930.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3927..4742)
                     /locus_tag="IWI97_002457"
     CDS             complement(3927..4742)
                     /locus_tag="IWI97_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2463931.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5004..5447)
                     /locus_tag="IWI97_002458"
     CDS             complement(5004..5447)
                     /locus_tag="IWI97_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBC2463932.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5478..5591)
                     /locus_tag="IWI97_002459"
     CDS             complement(5478..5591)
                     /locus_tag="IWI97_002459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2463933.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(5581..5919)
                     /locus_tag="IWI97_002460"
     CDS             complement(5581..5919)
                     /locus_tag="IWI97_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2463934.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5907..6284)
                     /locus_tag="IWI97_002461"
     CDS             complement(5907..6284)
                     /locus_tag="IWI97_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2463935.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6508..7701
                     /locus_tag="IWI97_002462"
     CDS             6508..7701
                     /locus_tag="IWI97_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2463936.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7867..8289)
                     /locus_tag="IWI97_002463"
     CDS             complement(7867..8289)
                     /locus_tag="IWI97_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463937.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2464 a         1696 c         1413 g         2829 t
ORIGIN      
        1 ttgatttctc ttcttttttg tttcaataga cgattttagt tattaataaa tgaaaaagca
       61 tttatattaa aatttgctaa taaaagatct acatattttt ctagaaatgc ttgatcgatt
      121 tcatcatacc ctttcgttac tccgcaatcg atatctaaaa caccgattaa attaccttct
      181 tttaccattg gcactacgat ttctgattgt gcggctgaat cgcaggaaat gtaatttgca
      241 tgggttttga catcatcaac gatcaatgtt tcttgttttt cagctgattc tccacaaact
      301 ccttttccca tcttgatacg tgtgcaagaa actcgccctt ggaacggtcc taaaatcaat
      361 tcattaccat cgaataaata atatcctgca aagacagtct caggcaaagt ctcattcaac
      421 aatgcagatg aatttgctaa atttgctaac cagttatctt ctgcttctaa taatccttgt
      481 tgctgtaata ataaaatttc gtatgcatct tttttctgtt tttctgtcca catgataaaa
      541 accgctcctt tttgatctat ggagaagatt atagctcagt ttttttgtaa acgctaggtt
      601 tcctcctcaa aaaaacgaga tacgtcaggt taatcacctt gaacgcatct cgctgtcttt
      661 ttctattctg cttgttggat ttctgctgct aaatcagcat aatcaatcgt ttcgtgaaca
      721 aaagtcggat catcaactgg aaaatgctgt acaagggacg agatgcggct aatctcaact
      781 tttccttcag ccacttcaaa atccattaca tgtccgccaa atgtatgtgt atcatcgata
      841 aaatgcaagt gaaatcccgc tgcagcaact ccttggaata attcaggcgt aaagaacccg
      901 actaatgttc cttggacatt gctttgttgg aattctggtt gagactctga aatttttgct
      961 agtcttctgt aaggtttttc ttgcttcggc atgatacgga tgtgcatatt tttgaataat
     1021 ccagttatct tcactgccaa aaataggttt tccccggctt ctcttactaa tatgtctttt
     1081 ttcaattgtt ctccatctgc agtttttgag acagaaaaac tagtatctgg cgtaaaatct
     1141 gtaattgctg catagggggt aagttcactt ccatctaatt gggcaactga gccatctgat
     1201 cttccttgat aagctacgcc atctaaaaag atgacttcac catcaagccc atgcaatgtt
     1261 cccaatccca gagttccttt ttctaatata tgagcaatcg tttctgttcc atccatcaag
     1321 ccagccatca aagctcctaa tgtcccgtgc tggtataaga ttttttcagt catgtcgaca
     1381 cttccttaat agaattgatc tggtaagatt gtttctccca atttctcatt atcacggtaa
     1441 tcaatcggaa tatcaataat tacgggtcct tctgtcgcaa atccttcttc taaagctttt
     1501 tctaattcct ctacacttgt agctcggatt cctttagcgc caaaagcttc tgcatatttc
     1561 acaaaatcaa ctggaccaaa gtctacacct gacgcccgct gatatttcat tttttcttgg
     1621 aactctacca tattgtagtg tccatcattc caaatcaaat gaacgatatt caatttttta
     1681 cgtaccgccg tttctaaatc ctgggcagag aataaaaatc ccccatcgcc ggaaactgat
     1741 acgatttgtg tatttggacg caccaatgca gcagaaatcg cccatggcaa tgcaacacca
     1801 agcgtttgca taccattact gaataacaaa tgacgaggtt cataacttct aaagtggcgt
     1861 gccatccaaa tataatgact acctacatct acggtaacag tcatatcatc tgttactttt
     1921 gactgaagcg tgttaataac ttctaaagga tgcaagatac ctgcttctcc ttttgattga
     1981 acaatgtccc gctccgttaa ttctgcttga agagatgcta aatattcttt tgcatcttct
     2041 gacacttgtt gaggctctaa tgaacctgtc aataaatcaa gagtggctga gatatcaccg
     2101 atcaattctc tttcaggctg cataaaagga tcgatttcag ctggcgcttc atcgatcaca
     2161 atgatccgcg catctttttc cgcattccag tttcttgctt catattcgat tggatcatag
     2221 ccaatagcaa taactagatc acttcgtttc aaaagcatat ctcctggttg gtttctaaat
     2281 aaacccacac gaccaaagaa atgcgcttct aattcacgtg agatcactcc agctgcttgg
     2341 aatgtctcta caacaggcaa tgctgtcttt ccaactaatt gcctgatggc taacgtttct
     2401 ttttcacttg atcctcgcat acctactaat agtaccggta gttttgcttc tctaatgcgt
     2461 tcagctaggt aacggatatc atctgcagaa gcagaaccta atttaggatc gctcaatggc
     2521 ttgatgacat tcccttggac aggtgcatcc actacatctt gcgggatact gataaagctc
     2581 gcgccttttt ttgaactctt agccatacga taagcattgg caatcacttc tgaaattgtt
     2641 tctggatctt ggatttctgc actgtatttt gtgattggct ggaacagtgc tgcattatcc
     2701 atgctttgat gggtcaactt caatagatcg cttcgtttta cttgtccggc aattgctaaa
     2761 actgggtcac cttctgctgt cgctgttacc aaacctgtag ctaagttact agcaccagga
     2821 ccacttgttg cgataactac tcctggctct cctgtaatac gtccgattgc ttgtgccata
     2881 aatgcagcat tttgttcgtg acgagtcacg attaattcag gaccttgatc ctctaattca
     2941 ttaaatacac catcgatttt tgcacctggt ataccgaata cataatcaac atgatgatta
     3001 atcaaacttt ctactacaga tttcgaacct tgtttcattt tttcgtccat tgaacatcgt
     3061 cctttttatg ctttattttt aattattagt tagaaataat cacaaataat tgatacagtt
     3121 tctaaacctg tttcattcaa aagtatagca cgttttattt catttgttta gccatatgtt
     3181 tcaaaaaatg aaaaagaatg acagttttta cataaaaact gtcattctta attgaaagtt
     3241 tcaatttata aagtctacag atccatagtt ctcatcaatt tacgattgtt gaagtaaatc
     3301 aaaccaaata cgatagaagg acctagtaga atacaccatt ggggctttac gaaaaagaag
     3361 agaaacaaac ttgtcaatag taaaaaatct agtaaactca cataccattt tttcaacgta
     3421 tagtagaacg ctatccgtag aacttgattt ggtttcgacg ctggatttct cacctgaaaa
     3481 taccagtagt tgatgctaaa ggaaactgat aaacaagcaa gtaaaacaaa caacggaatc
     3541 aaccattttc cataagaaaa acgtataaaa aagaaacaat caaaaatcat tatcacacta
     3601 actatccaag caaatagcca ataaaaaaat ccctttagcc aaaattggcg aaacgtcttt
     3661 atgaacaaac gtactggtga aaaacttttt tcttttgcta attcttctgt catacctatc
     3721 atcacgatgc aacctggacc aataaaaagt aaagaaaaaa taaaggaaaa gacattcctt
     3781 ccatctatcg acaaaaggaa cgcagctgct atatacaaga tattgacaca ccaaaaacaa
     3841 aggctacttg ccaatgattg atacaaccaa taaaagaatt ccatataaac atttttgtca
     3901 aaacgctgct ctttcataat cacctcctat tccttcattc ctgatgaagc gatcccttct
     3961 acaaaatatt tttgcatcgc taaaaatata aaagcaacag gaagaacaga aacaactgag
     4021 gcagccatga ttaatgcgta ctctgcatca aatagcccaa taaacatttt caaccctaac
     4081 tggattgttt tattttccgt agaagacaaa taaatgaatg gccccatata atcgttccat
     4141 gtattaacga atgtgatgat cgtcaaactg gcaatcgccg gttttgtaag aggtaaaatg
     4201 atcttgcgat agattcccca ctctgataag ccatcgatcc gcgcactctc acataaagaa
     4261 tctgggatac tactgtagta ttgcttcaac aagaacacac caaaagcatt gaacgcttgt
     4321 aatagcacca acgaccacaa cgtatatgtt aaacccactt ttctcatcat aatgaactgc
     4381 gggatcatat aagactgcca agggacagca atcgtgccga tatatgctaa aaataaaata
     4441 tcgcgccctt taaaatttag tttagaaaac ccgtaagcag caaaactaga agtaaacaac
     4501 tgaatcaacg tgatcacaac gcttaaaaaa gctgtattct taaaaaatgt cactaaagga
     4561 atacgagacc aaataaccag ataattctct aaatgccaag ttttagggat ccattgaatc
     4621 ggaatcgtga acacttcatt atttgctttg aatgaagcag aaaccatcca taaaaaagga
     4681 atgatcgtca ataaagctaa tacaatcagc agaatcatac ccaaccattg aataaagggc
     4741 atgttttttg aacgaatttt tgcttttttt ctttcatttc gaacacctcc aaagttataa
     4801 tcttcatttc tttgtaatga ttacaatcca gaacaacggt tattatagct gattcatttc
     4861 cgttcttctt atgtttgtaa gcgcttattt aacaagttat gtaaatctct ttattttttt
     4921 tgatttaaaa atgaaaaaat acaaaagcaa gactgtcata cgtctcgctt ttgtattgaa
     4981 atttcttcga ttagagatac ttattactgt tcaaaagctt ctttgaaacg gtgaatcaat
     5041 tcattgacta caggtgataa aatccgattc tttttccaga ctagcatgca gttcgtttca
     5101 atcattgggt caagcggaac gaatgtcact cgctttgaaa gatgacttac agcaccttct
     5161 atgactaaag cagcacctac ctgattctca attagcgcaa atacattgaa aatcaaattg
     5221 tacgttcctg tgatcagcaa ctgctccttt tttatgccta accagtcagc aaacattcgc
     5281 tgtatttctg gacgctttga gatcaaaaga ggtacattcg caaagtcttc ttttgttatc
     5341 acgtcttttt ttgctaaaac agaattttct aaaatgagca atccccattt ttcctttctt
     5401 ggtaacaaaa tcgtttcgta tttctctgta tctactggat ctagcaaaat cgctaaatct
     5461 aacaggcttt tttctagtca ctccgtgata tcatcacttg ttccgctgtg gatatgaaat
     5521 tgaacgctgg gataatcacg gatcaacttt tctagtaata gtgccaatgt attcgaatta
     5581 tcagcttcca cacagccgat cgtaaatcgg ccgctgaata attgtttttt tctgttctcg
     5641 aattcttgag tggtttgatt cgttaaatct aaaatttctt ctgctcggct tttttagaaa
     5701 ataacctgct tctgtcaaga tcattttgcg gttttcgcgt acaaacagtt ctgttcctag
     5761 ttcctcttct aactccttca gttgtcggct taaggttggt tgtgtaagat gtagaacttc
     5821 tgcggcttga gagattgttc cttcttctgc aattgtccaa aaatatttta gtaagcgtaa
     5881 ctccatagcg atgctccttg atttttttac ttgatacatt ccattttatt tctagataac
     5941 acacgatcat atttcttgat ttttccttgt aaacgttcca aacattcttg catttcagca
     6001 atttttttcg tcaaaagttc actttcttca atcaaaagct tacgccgctc aaatacagtt
     6061 gcttctcctt cgctagatag gcgaatatat tcaatcaaag actcgatcga taatccggca
     6121 tttcgcatac agatcgtaaa gtcgacccat tttaggtctt cttctgtata atcacggatt
     6181 ccgccttttg tccgattaac tggtggaatc aacccgattc gttcataata gcgcaatgta
     6241 tcggccgaaa tgcccttttc ttcactgact tgtttgatat tcatctgatc actctttctc
     6301 ttaatttctt gccactttac ttatgtattt tatttattct acaactttaa gtgtactcca
     6361 agtcaatact catttctatt aaaactaagc tggattatct atatcggttg tttgtgtatt
     6421 atttcaaata aaaaagtctt gtttttttat tcttttttgg atatactttt aacaatttag
     6481 ggaaatttta ggaaaagggg tataaaaatg acaacgtatc agcgaaagtt attattatcg
     6541 acatcggcgg gaattgcttt agagaatatg gatattatgt ttttagcttt ttcattatct
     6601 tcaatgatta cttcttttca tattagtggt acgcaagcgg gtttcatttc aacgatcact
     6661 aatttgggga tgctcattgg aggtattttt tttggcatca tagctgataa atatggacgt
     6721 gtgaaagtat tttctcaaac ggtccttctg ttttcgattg cttctttatt gatgtatttt
     6781 gcttcaaaca tctacttggt ttatctcttt cgctttatcg caggtgtagg agcgggagga
     6841 gaatacgggg cgtgtatgtc cttgatttcc gaaagttttt caaaaaaaca aattggacga
     6901 gcttcttcgg ttgctggtat tggtgcgcag gtcggtgcgg ctttggctgc gattttagct
     6961 gctgtgatca ttccaacgtt tggttggaag atgttgtatg tcgtaggtgt actgccggtg
     7021 ttgatggtct tgttcattcg tcgcggcttg actgaaccaa aggaattcaa atctgtacaa
     7081 tctgccgaaa agaaaaccaa acttagcaga ctgtttgaat caaaacatct tgcttggcaa
     7141 acatgtggcc ttagtatcat ggtaactgtc caaattgccg gctattttgg gttaatgaat
     7201 tggctgcctt caattatgca aaatcgatta gggctttcgg tttctgggtc ttcattgtgg
     7261 atggtcagta ctattttagg catgtctttg gggatgatga cttttggcat aatcatggat
     7321 aaattggggc ctaaactagc cttttctttg tttttaattt gttcatcctt atctgtctat
     7381 ctacttgtgt tagcccatag ccagtggagt ttggtagcag ccgctgtgat agtcggctac
     7441 tttatcaatg gcatgtatgg tggatatggc gcagtgatta gtagtttgta tccaacagaa
     7501 atccgcgcga cggcgaataa tttcattatg aaccttggac gagctgttgg tggtttttct
     7561 tctatcgtca tcggtttctt gatggatcat tatagtttaa ctgccgtaat cttgtttttg
     7621 agcggtattt acctagtcag tctgctggtc ttgacaactc ttactggtgt tacagcatta
     7681 agggaaagct tgcagcattg atagttgagt ctgtaaatga caagaaagag gtgtggcaaa
     7741 agcttgcatc tgcggtaata agaccagcca aatcaaaaac atgagacact gtttccgttt
     7801 ggctggtcgt attacttgat gcagggcagc tttttcaact gctaatttcc ttctatctgg
     7861 cggctactat tctttgacgt ccgtcatttc aaagtacttt actaataact tagcataacg
     7921 aaaaactgtg gatacactcg tatattgagc gtctgcataa tcttccaaat ttttctcagg
     7981 atgttgatac atgaattcaa tcaaatgata attatatgat tttctgtaga aatgaagata
     8041 aataatgtag attgggaagt tctcctgctt ttccatagaa aagcgccgat tattctcatt
     8101 cttcaaaatg accttatcag gatatttttg gaacaattct tctaaatctg ttagataatt
     8161 caagatagac ttacttgata ccttcaattc gttttgtagt tcttctattg aacaccaacg
     8221 ttctgtttca agcagtaaat gaatcatttt aattttcttt tgagaaactt ttgttaaaaa
     8281 agtctgcata ttttctcctc ctgtatctac tttactatac tacatacaag aaaaaaaacg
     8341 gatacaaata tcatattgtc acaccgtcaa tagtgcataa acctgttatt atttctcttt
     8401 tt
//
LOCUS       DADXTH010000099         8303 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.101, whole genome shotgun sequence.
ACCESSION   DADXTH010000099 DADXTH010000000
VERSION     DADXTH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.101"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1304)
                     /gene="celB"
                     /locus_tag="IWI97_002464"
     CDS             complement(<1..1304)
                     /gene="celB"
                     /locus_tag="IWI97_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBC2463938.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLL"
     gene            complement(1304..1822)
                     /locus_tag="IWI97_002465"
     CDS             complement(1304..1822)
                     /locus_tag="IWI97_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463939.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1838..2164)
                     /locus_tag="IWI97_002466"
     CDS             complement(1838..2164)
                     /locus_tag="IWI97_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBC2463940.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2177..4156)
                     /locus_tag="IWI97_002467"
     CDS             complement(2177..4156)
                     /locus_tag="IWI97_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBC2463941.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4177..4491)
                     /locus_tag="IWI97_002468"
     CDS             complement(4177..4491)
                     /locus_tag="IWI97_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBC2463942.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4659..4952
                     /locus_tag="IWI97_002469"
     CDS             4659..4952
                     /locus_tag="IWI97_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBC2463943.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5030..6142)
                     /locus_tag="IWI97_002470"
     CDS             complement(5030..6142)
                     /locus_tag="IWI97_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBC2463944.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6295..6519
                     /locus_tag="IWI97_002471"
     CDS             6295..6519
                     /locus_tag="IWI97_002471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463945.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            6529..6708
                     /locus_tag="IWI97_002472"
     CDS             6529..6708
                     /locus_tag="IWI97_002472"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463946.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6902..7072
                     /locus_tag="IWI97_002473"
     CDS             6902..7072
                     /locus_tag="IWI97_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2463947.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7496..8236)
                     /locus_tag="IWI97_002474"
     CDS             complement(7496..8236)
                     /locus_tag="IWI97_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2463948.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2592 a         1706 c         1367 g         2638 t
ORIGIN      
        1 tttaataatt tattatcata gaatttgata aatgggaacc agataatgaa tgcgatgatt
       61 gcacaaatga ctgctaacac agcagctttc caatcaccac cactaccaac aaatgctccg
      121 atcccgactg gtgaaggcca cggcatctgt gcaagcatcg gtttgacgat ttccagccga
      181 atggcaaaat aagcgagtgt agcagaagcc atcggagcta agaaaaacgg aaccgctaaa
      241 tagggattgt agacgattgg catcccgaaa atgattggtt cattgatatt gaataatcct
      301 ggtacaagtg aggcttttcc taacactttc agctgatcag atttcgcgag gaatgcaata
      361 aagatgatca atcctaaagt cgcacctgat ccaccaacgg ttacataaga attgttgaat
      421 tctcctgcca acggataatt tgcgccttgt gcgttagctg ccatattagc taatacgatc
      481 ggggtcaaga aagaagtgat gatattcgct ccatggatcc ctacgatcca caatgcatgg
      541 atcaagaaat aaatcaccat gacaccaagc catgtattcg tcaactgcgt aacaaaccca
      601 aatgggatag caatcatttt gaagatatcc gttcctagtg caactaataa tccattgatg
      661 atcatcacca caaacgcaat gacaaatgtt gggatcagag cagtgaaaga acgagaaact
      721 cctggcggaa caacatctgg catttttatg acccaatttc tcttgacaca catacaatac
      781 aattcgaccg caataatcga cataataata gctgtaaaga ttcctgaagt tcctaaacgt
      841 tcgacaaaag agcccatcct aataccattt actacagttt ctgtctcatt gatagaagca
      901 atcaagctga ttttaccatc agtcatgatc aattcaggga tacacataaa gaatgcaaaa
      961 acagatagta atgcccccgt gagcggatta acatttaatt tttgttcatc tcgttcaatt
     1021 gacgttaatt cataagcaaa cacaatggca aagtaaatag ataaaatccc catcgtcatt
     1081 gtattgacga tcatgtacaa gtcactaact ttgaaaaaag ttgcattaaa aaatccttct
     1141 aaaaatggga acggtaatgg caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg
     1201 aaaggaatag aagccatccc cgctgccatg acagcccgaa cgattttaag ttgcgcaatt
     1261 ttccccatag gtcccatcag gtatttctgc aaaaaatcaa acattagtta ttctcctttc
     1321 ctaaatataa cgctttttca tattgtttaa tacggatata ctgttggtct tggttgagtg
     1381 aaattttctt taaacgatac tgtataaatg cactggcaaa aataccgctt acaacaagaa
     1441 tgaaaacaaa taactgtcct ccattgttat ttgccataaa aggatacaag aatgtaaatc
     1501 ctgaggtaaa caacactgga atcactaata aattaaagac actctggaac aaaaaatacc
     1561 actttgtcca tggaacgttc gtttggtgtt ttcgataaag ctttacttgt tctcctactg
     1621 caggcaaaat agctaaaatc agaatcatcg gtaaactact tcctaaactt ttagctgata
     1681 gaagcagaat catccattgc atgttcatga aaaagaaaaa ggcagtcagg taacgtacga
     1741 ttagataacg attgaaatac atgtttttca aactaagttg ctttttttct aattcgattt
     1801 tcctttttac tgtatcctgc acaatgatcc ttcctttcta tttatttttt tgatacaaat
     1861 aattcatttc ctgtgcaact tccaataatg tcatcgtcgt catcagatga tcttgtgcat
     1921 ggaccatgat aatttccatt tctatttttg tcccgctggc atattctttc agaagttgag
     1981 tttgtgactg atgcgcatga agaatctctt catttgcttg tcgcaacttc tcttctactt
     2041 cttcaaacat ctctttacgc atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc
     2101 cgctatggaa aatgatatca aaagaagtca cttgtagtgt ttcactgttt attttttcag
     2161 ccatctgttt tcctccttat tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa
     2221 taggttcagc taagatttcc tgctgtactt ttagctggtc aactaaccta acgattgctt
     2281 tagtcacgac cgtaattcct tcattttcga cataagaagg cgaaactaaa aaaacaaaat
     2341 gaatttcttg atattgatcc cactgcatcc cagaaggaat caatgcaacg gctacctttg
     2401 tttcgattcc aactggaaca gcgggatgtg ggactgccac tgtatcactg aatatgattt
     2461 gtcccatatt ttcacgttgc tggatctgct ttcgcatttc tcttatatat ccttcttctt
     2521 catttatgct cagcttttgc agcaaatcat gaagaatttc ttcctttttc ggttcgtacg
     2581 tataagtctt gaaaaatttt tcagacaact gttcggtgta aatctgcttt ttttgctgta
     2641 gagaaaatgc tggtatcggt ctatctgagg aatgataggg tatagattct ccaactacct
     2701 ttcgtatttt acgaacatca tcttcattca aaaaaatact gacatgtaaa actggaattt
     2761 tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag gtcgattcct tttaaagttg
     2821 tttcattgat ctcataatag ccttttacac ttaccactgt aatattttta ccgaattcac
     2881 tgactacgcg attctttaat agttgggcac tgccgtaacc cgttgcacag ataatcaatg
     2941 catgtagttt atgcgcagct ctttcttttt ccaatgccgc catgagatgc aaagccaaat
     3001 aggcccattc atcttcattg atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc
     3061 tttttactgc gtcaaagact ttgggatttt ctttctttat ctcctcagtc aatgggttct
     3121 caatgcgaat acctcggctc aatcgaatca tcatcggtga caaatgatcg agtagtccat
     3181 tgaaaagctg gagatcctct actaatgaaa aatgaaattc ctgagacaat cttttcagaa
     3241 ctttcgctaa ttcctttgat agttcctgat cattttgatt ttctttgtga ttcgacttag
     3301 ccatgagatg taatgtcaaa taagcaattt cttcttcggg aaacgataca cctgcgatta
     3361 cctcaatacg ttgaacgatc cgctgcgcca cttgatactc tactcgctta gaaacttctt
     3421 gttcgatgcc taattccttg atttcaaacc cttctcggag ccgtttgatc cccaaagcca
     3481 agtgtaaagt cagattctgt atgataaaat ccgaaacttt taacttagct tctcttgttt
     3541 catcgagaat gataatgact agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc
     3601 ctaaatattc tttcaatgaa tttccataat ggtttccaaa aaaagtatcc atgataaaat
     3661 gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg accatgctta gaaacgattt
     3721 ttagtgaata tggaatcaat cgctccttga tttcttgcat gtcctttgtc aaactggaac
     3781 gactgatata aagctcttct gccaaatcat ccataaaaag aatggcatct tccaaaagca
     3841 attggttcaa tatatacttt tgtcgatctt cggactcata aaatccttgc ggttctctag
     3901 catatccagg tactatttct tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa
     3961 tatgcagttg gtagccataa cctggtttag caatgatctt acctccgttt ttttctacta
     4021 actctttgat ccgctgcaag taattacgga ttgtccgatc agataataac aattcagaag
     4081 ctaaacgttg actggtgaca tactccttct tgtgatcaat caaatatctc aacagcaatt
     4141 tttccttctg tttcattatc atccactcct attccatcat atgttctctt cgatcacctc
     4201 tgctaacttc tggatgcctg tagggattgg gacatacgct tggaaaggga tactaacaac
     4261 aggctttcca acagattctc ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg
     4321 gctgatcaga aataaatcaa aatcactatt cttaatcata ttatcgcctt ctgtagctga
     4381 aactgcatct acgagtacct cttctctttt ttgcttaaag aaatccgttg tctttgaggc
     4441 catcatcgag gatgacattc ctgccgcaca aataatcaat gcttttttca taaatcaatc
     4501 actcctttta tttttataaa ccaagcatat cttgtaagcg ttttctattc aaaatatata
     4561 tttccgtagc attgcggaaa aaatgtgtat catatcttga ataataattt ctggcaccgt
     4621 gatatactag tgatagaatt ttttcaggag gggatttagt gagtatcaca gtgaatattt
     4681 tgtatacaag tacaaatgga caagccagaa aatttgcaga agaaatggtc gaaagaaaca
     4741 ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata tgactattac tttccactag
     4801 aagatccaga atctctttta ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc
     4861 atcacaaatc cgaaatgatg gctcaaataa acgaactccg taaaaagtat aaattaaaaa
     4921 tgaaagtcga acgtttcact gatttaccgt aaaaagatac acatggaatg atagcaattt
     4981 atcatccatg tgtatcttca ctttttcctt attttttatt cgatctcatc taatgattca
     5041 aaccgtgttt ttctcttatt tttcggataa gtatttcagt caccccaata tacatgattc
     5101 ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga
     5161 ataaactggc agtgattgcc aaagcaccaa aagaattaaa aactgtttta tatctatagg
     5221 tagaacaaac acctaaagct agaccaccta atataccgac ccagttaaaa ggaataaatt
     5281 ggctgatacc aataaataaa acacatccaa tcaatgagcc aatgattcgg tctactgctc
     5341 gttctttgaa accaatcgac atcaatccat agctagatac aatagaagaa aatgcaaacc
     5401 ctgcccacat caatcgctga aaagggacat actctcctgc aacgatcaaa agactaatgc
     5461 caaatgcata gatgagcatc caaatttttt cctgcgaata catgcctttc aaaaaaatct
     5521 ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa
     5581 accaacaaaa aaacaaaaat aataagctac ttgtttgtgt aaagaaattc ttgttcaacg
     5641 aatctttagg taacgaataa acaataaaga gatatgaaaa gccgtacaaa gaagcattcc
     5701 ccattttggg atcactagcg gttgccatta atatgctgct tagtaaaata aaatgaatca
     5761 acatgcccat aactgaccat ttgatcagct ggacatacgg tgctactaat agtgagaaca
     5821 tgacgattgc taaacctatg atcgaatggg aaatcttata accaaagtca acaaaacgaa
     5881 tactcatcaa catacagaat aaaaccacag cgtatggttt atgcttcgat tcaaataatg
     5941 ttgtaattat aatgataaaa caaatggcaa acaatacgat gaataatgat cgtaaaaaca
     6001 atgaacaaat atacatgcac ttttcttttt tagaagctgc ttctcgtatt ttttgtttta
     6061 aaataaacgg atctagttgt agagactgat aaaaatccac ctgttgtcgt cctttctttt
     6121 tacttttatt ttttacagtc atctatgaca ggttgtaaat actatggaac atcatatggc
     6181 gttgtcattt tcttgtcaac tatttttcct attcatgttt ttataaaaaa acatatcgtt
     6241 ttcttgtatg attaaatcac cacaaaataa acaactgaga gaacgatatg ctttatgaat
     6301 ggttctatca aaaaaattct gagatcaata gacaaaaatt tgatgattac agaggtctct
     6361 tacgaaacct tacagaagaa aaaaacattg atcgttgctg cagttctctt ccctgctcca
     6421 tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt aatggtggtg
     6481 aaaaacaaga agaaggaaac cattgtccac tttgaataat ataatcatat gccaatggtt
     6541 atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc gaagccattg gaacgctcaa
     6601 agttatttta tccgtccaag gcactcgatt tcaaatcatg tcagacataa aatcacttct
     6661 ttactgactg aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt atctttgaca
     6721 accgttattc gcacattgaa agagttgaga agctatttac caaagtcttc caaaagaatt
     6781 ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt ttccgttgag gataaactga
     6841 gttttatttc cgcggatggc gagacagaaa aattaatcga gaaaatttac ctacacaaaa
     6901 aattcctcga ttaacaaact atttccttag ctgcactaat cctaaagaaa tagaattctt
     6961 agttacagac atgaacgcca cctactttca gctcaccaaa catgttctac caaatgcgaa
     7021 attggtgatt gatcgattac ttggtttttc cacgcctttc aaggaggcgt aacctttatt
     7081 cacgagttgg ttgaagcttt ccgagacaaa agacccgact tgtttttctc cttattgaca
     7141 aagagccatt aaaactagag aaaaaattac aactgatcgc tgattatttg tagaaagaaa
     7201 atcaaagtat tacccaataa acttgagtca ttaggcaaaa aacactgcga aactcaagaa
     7261 tgattttcgc agtgtttttg tatatttttt attaactagt attactacaa aggtttattt
     7321 ttcacttctc aacgcagcac aaaattgtct ttacttattt actggttggg ttcaaccttc
     7381 tctactccga tactaatatt tgtttgtata ccccttctaa aatcggtagt ttccattcaa
     7441 ttgatgggta ctagttgatt ttaaaaacta tgcttattca attttttatt ttagctcata
     7501 tatttcgttt ctttctaaat tttatactga tgaaaacaag tagcacaccc aatattgaca
     7561 atataaactg gctaatgatg gtttctccag tttttggaag atgcttattt ccactgcttg
     7621 ttggattgtt tcctcttata tctgagctat cagcaacttt tctattctct tttgttaaaa
     7681 agatcgccac ttcttgttct ttttcgatct tgaaagttac tggtgtcgca tccaattcat
     7741 accccgttgg cgcttgagtt tctaccagct gatatgctcc tggtgctaag ccatctatcg
     7801 ccagttttcc atctgctcct gtggttagac ctgactgcag aacggttcca ttggcatctt
     7861 gtaactcaaa aacagctcct tgcaggactt ctccgctttg gtcatcgatc tttgtcaaca
     7921 cgacaccgcc tggtgtaagt cgattttctt tcgtcaattc taccaccgct gtctgacttc
     7981 tttcgatctc aaactcgatt ggtgtcgcat ccaattcata ccccgttggc gcttgggttt
     8041 ctaccagctg atacactcct ggtgctaagc cgtcaatcgc cagttttcca tctgctcctg
     8101 tggttagacc tgactgcaga acggttccat tggcatcttg taactcaaaa acagctcctt
     8161 gcaggacttc cccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc
     8221 ggtttgcttt cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgatcg
     8281 gtgtcgcatc taattcatac ccc
//
LOCUS       DADXTH010000100         8229 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.102, whole genome shotgun sequence.
ACCESSION   DADXTH010000100 DADXTH010000000
VERSION     DADXTH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.102"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="IWI97_002475"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="IWI97_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBC2463949.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="IWI97_002476"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="IWI97_002476"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBC2463950.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="IWI97_002477"
     CDS             2592..3377
                     /locus_tag="IWI97_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2463951.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="IWI97_002478"
     CDS             3390..4241
                     /locus_tag="IWI97_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBC2463952.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="IWI97_002479"
     CDS             complement(4365..4817)
                     /locus_tag="IWI97_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463953.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5282..5650)
                     /locus_tag="IWI97_002480"
     CDS             complement(5282..5650)
                     /locus_tag="IWI97_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBC2463954.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5800..6282
                     /locus_tag="IWI97_002481"
     CDS             5800..6282
                     /locus_tag="IWI97_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBC2463955.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6319..7329
                     /locus_tag="IWI97_002482"
     CDS             6319..7329
                     /locus_tag="IWI97_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBC2463956.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7444..8157)
                     /locus_tag="IWI97_002483"
     CDS             complement(7444..8157)
                     /locus_tag="IWI97_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463957.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1667 g         2440 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa gaaattcgtt ctctcctaaa gtgatattat
     4981 atttaaatat acctgaaaat aatgggcagt attatgaaag atcactgata ttttctcgta
     5041 ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt tactaagtgg
     5101 aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc cttataaaaa
     5161 tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat aggattgaac
     5221 aaaacctgta taagctgatg aatagtttcc tcgccagtaa aaggtttatt cgtataaagc
     5281 tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa atcaattgtt
     5341 caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta tccgtatata
     5401 cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt tccggtgcga
     5461 tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg tcagtaaaca
     5521 taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt atcttagcaa
     5581 aaaggtcttt gatcttttgt tgatgttctt tttctttgat ctgattgatg atcggctgaa
     5641 atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca gaaaaacaat
     5701 agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata tttatatgat
     5761 aagatgtctc aaataataaa atagaaaaga gacataaaaa tgattgactt ggcaaagaaa
     5821 ttcttacacg aaaatatata ttttgagttg tatcatcatc gagccattta tacaaatgaa
     5881 gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa aagcttattc
     5941 ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa acaaacggat
     6001 ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg
     6061 gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga aaaacagatt
     6121 ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc accaggtaga
     6181 ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca gctacttgag
     6241 atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat
     6301 acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc aacgattggg
     6361 gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga atcagcttta
     6421 aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg gacgcaagat
     6481 aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat gatgcaagat
     6541 gaaaatatcc aaatgatctt tccgccttgg ggaggagaat tattgattga gattctcgat
     6601 tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga tattagtacg
     6661 ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac caaccttatt
     6721 gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca
     6781 agagaaggag aatcgattct acagcaacct tctgaaaagt atcaaaaaga gtggccccat
     6841 gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca atcagtcagc
     6901 ggagatgcag taaggatgaa gggacgatta ttaggtggct gtattgatac gatcagtcat
     6961 ttaatcggta ccccttatgg agatgtccaa tcttttaaaa gaaagtataa tgatggaaag
     7021 ccgatcttgt ggtttctaga aaattgtgag ctatcgtcag ttgatttaag aagatcactt
     7081 gttcagatga aactggcagg atggtttgat ggcagctcgg ggatcatgtt cggtagaagt
     7141 gcagcagagg atttgttaga ttataaaatg aaggatgtat atgaagatct gtcaaaggaa
     7201 ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca actgacattg
     7261 atcaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt tttgcaaacg
     7321 tttatttaaa aaattttttc ctgccttgga gtttatcaaa gaccaacaaa tctaaataga
     7381 aaagaaaatg aaagaagctg acagtatttt gccagcttct ttttgtatga tctattagaa
     7441 attttaatta tccataacta tcacattgat cagctgtttc acatcatttg gattacctgt
     7501 aggaatagct actacagatg cattatcaat aatttcttga gttgtttctc taatgaacgc
     7561 tggataagat gtttgatctg caggcatttg ccaatgtgaa ggaactggat aactagtgat
     7621 tgtcccgtca tggttacttg aatacactaa aactccctgg taactgtatt tcccagtgag
     7681 cataaccaca tctttcggat agactgcact ccctgcatca taaggggcga taggtgttcc
     7741 tgcaggataa tgaataacat gtagttcgaa atcacccgat gtatctctaa aagtattacc
     7801 tctaattgct agccaaacac gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg
     7861 atctattgac tgagaagact gtgaggaagt tgtgtctatt gttgaagaag aaatatttgt
     7921 ctcaactgta ctactagatg tagaagaagt attgggctct gagactttac tgcttgttga
     7981 agatgtattc gtttcagtag aagctgattt ctgagtactt gatttttcag ctgaactggt
     8041 ttgtttgatt gaacttttgt ctgtggtcgc agatggatca ctattttctt tactaggaga
     8101 cgtacatcct acaaggaata aagagaccaa caataaaata aaacctaact ttttcatata
     8161 aaatacctca ttattttttt aataccagta tagcataatc ctgaattttt gaatccgtaa
     8221 aaccttgtc
//
LOCUS       DADXTH010000101         8073 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.103, whole genome shotgun sequence.
ACCESSION   DADXTH010000101 DADXTH010000000
VERSION     DADXTH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.103"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            120..1757
                     /locus_tag="IWI97_002484"
     CDS             120..1757
                     /locus_tag="IWI97_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463958.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1902..2642)
                     /gene="truA"
                     /locus_tag="IWI97_002485"
     CDS             complement(1902..2642)
                     /gene="truA"
                     /locus_tag="IWI97_002485"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBC2463959.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2833..3468)
                     /locus_tag="IWI97_002486"
     CDS             complement(2833..3468)
                     /locus_tag="IWI97_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBC2463960.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3615..4856)
                     /gene="sstT"
                     /locus_tag="IWI97_002487"
     CDS             complement(3615..4856)
                     /gene="sstT"
                     /locus_tag="IWI97_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBC2463961.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5054..6050
                     /locus_tag="IWI97_002488"
                     /pseudo
     CDS             5054..6050
                     /locus_tag="IWI97_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6066..>8073
                     /locus_tag="IWI97_002489"
     CDS             6066..>8073
                     /locus_tag="IWI97_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBC2463962.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2645 a         1503 c         1249 g         2676 t
ORIGIN      
        1 ctattttcag tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag
       61 cactagtatg atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa
      121 tgaaaaaaaa caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt
      181 acgtcatccc tctattcatg tctatgggaa tctatcatag tgtcaatcag gatacttact
      241 ttcatttatc tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata
      301 acttcgatca tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag
      361 cttttttatt ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt
      421 ttatcacttt tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc
      481 gttatatttc gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca
      541 tattccatcg tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa
      601 tggggtgtta cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca
      661 tgacgctagt agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag
      721 gaacgttgtt tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt
      781 taaaagctac cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac
      841 aatctagagc acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta
      901 gtgatatgct gacacacatt ttaaataata actataacaa ctatactatt ggattattct
      961 tatttctcgg attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat
     1021 ttattttctt tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac
     1081 tttttaacca tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt
     1141 ctttatttat agcctatacg atgagtatag tgatctatta tattttttct actaaaaata
     1201 attcatggaa aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa
     1261 gcaattctat ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg
     1321 ctatagctga aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg
     1381 atatgattaa taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt
     1441 ttatgagtaa caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga
     1501 cgacacctat ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa
     1561 caaaattatc taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg
     1621 ttattactta tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt
     1681 tgattttatt tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag
     1741 tacaacattc tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg
     1801 ctaaaaagtc tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt
     1861 tatactgtga aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca
     1921 tccaaaaaca gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca
     1981 aatatttcat cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc
     2041 atgataatcc gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt
     2101 tctccctttc gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt
     2161 ttcaatgcag aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc
     2221 agcttgtcca tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa
     2281 ggaatcacgt tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa
     2341 tggaagcgct caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga
     2401 ttcataaagt cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact
     2461 tgtcctcgcg catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca
     2521 tttgtcatct gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca
     2581 agtctttgcc atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc
     2641 atattttagt ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag
     2701 cacaaaatat agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata
     2761 tatatttcag aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg
     2821 acggctcaag ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt
     2881 aaagaaaaaa ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat
     2941 agaaggaatt cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc
     3001 aaggtataag tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac
     3061 tggaaacaat aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga
     3121 gacattccac gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc
     3181 tataccatag aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac
     3241 aaatgcagca tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca
     3301 aataagaaac gcttttctta caggactgga aacctctcca aatacactga tcacgctctt
     3361 catttgatct aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag
     3421 cacgcctaat aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca
     3481 ataacaatta cctatgcttt taattgttat tataagcgac tttatcttga tttaacagaa
     3541 acctctattt acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac
     3601 taaatatcta acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt
     3661 tccccacttc ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac
     3721 gaatttaagg cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg
     3781 atttgcatcg cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac
     3841 agtgaaccgc ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa
     3901 aggactttca aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc
     3961 aaggaaatgg taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg
     4021 gaataagact cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt
     4081 gcggcagaac ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc
     4141 aatggatagg gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg
     4201 acaaacagca tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagct
     4261 attccagtcg tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca
     4321 ataatcactt gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc
     4381 gattctgaac gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg
     4441 taattcgcat caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat
     4501 aaaacacttt ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga
     4561 acagtaaaaa ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa
     4621 tataagatca aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac
     4681 gccatcgtca agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac
     4741 gttcccaata ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact
     4801 aaaataccaa caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga
     4861 ccaccctttc acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata
     4921 attttctaaa aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg
     4981 tctacacgtt gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag
     5041 gaaacgtttt cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat
     5101 aaagaaggag agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca
     5161 gcgtatattg atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta
     5221 catctataca tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat
     5281 gggttaccaa tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc
     5341 ctttatattc ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca
     5401 gcttgaatat tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt
     5461 atgctgtttc ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata
     5521 aattatcgaa aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct
     5581 gctctactct atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga
     5641 ttttttattt atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc
     5701 gctataagct tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga
     5761 tgacacaaat ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac
     5821 catattctct cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat
     5881 ctgataaatt aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc
     5941 aagttatatg taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat
     6001 ttccaaactc agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat
     6061 tagcagtgtc cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc
     6121 gtttcatcga atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa
     6181 gaattcattg gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct
     6241 ttaccttcgt gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg
     6301 ttgccttaaa aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta
     6361 tgggattttt cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta
     6421 ccttgatcat taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag
     6481 agcctgtctt tccttcagat ttaaaaatac tgaccggatt gtcagaacta ttatcgatgg
     6541 ttagcccagt cttattaatc gtgggcggat tgatccttct cctattattg attactagta
     6601 ttattgtaca acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat
     6661 ttatcagtat cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa
     6721 attcgccttc tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc
     6781 aaggcgccgt aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa
     6841 tcatggaaga acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca
     6901 acacaaaggc agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg
     6961 ttctcaattt aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta
     7021 cagaccctat tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat
     7081 ccgtcggtta tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta
     7141 tcagtaacct gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa
     7201 taagtccaaa tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg
     7261 ctctttatcg cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag
     7321 acagtccgga taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg
     7381 attcagcata caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca
     7441 tccaattatc cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact
     7501 acactgctga aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc
     7561 aaggccttca ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg
     7621 ataaaccgat tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg
     7681 attcgaaaaa atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt
     7741 atagtcgtga acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg
     7801 ctatggctct tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag
     7861 tatctaatca tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta
     7921 atggaaaaca ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg
     7981 agaaacaaaa agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta
     8041 accagtacag tgcaacatgg gcgacacaaa aaa
//
LOCUS       DADXTH010000102         8022 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.104, whole genome shotgun sequence.
ACCESSION   DADXTH010000102 DADXTH010000000
VERSION     DADXTH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.104"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            109..1848
                     /gene="yfcC"
                     /locus_tag="IWI97_002490"
     CDS             109..1848
                     /gene="yfcC"
                     /locus_tag="IWI97_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBC2463963.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2005..3189
                     /gene="iadA"
                     /locus_tag="IWI97_002491"
     CDS             2005..3189
                     /gene="iadA"
                     /locus_tag="IWI97_002491"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBC2463964.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3300..3503
                     /locus_tag="IWI97_002492"
     CDS             3300..3503
                     /locus_tag="IWI97_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBC2463965.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3604..4266)
                     /locus_tag="IWI97_002493"
     CDS             complement(3604..4266)
                     /locus_tag="IWI97_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2463966.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4279..5781)
                     /locus_tag="IWI97_002494"
     CDS             complement(4279..5781)
                     /locus_tag="IWI97_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2463967.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6329..8008
                     /locus_tag="IWI97_002495"
     CDS             6329..8008
                     /locus_tag="IWI97_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBC2463968.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
BASE COUNT         2482 a         1426 c         1734 g         2380 t
ORIGIN      
        1 ttttctaata aattattcat gtggaaatgt catttttact gtataattca tttgattatc
       61 cattttttat tcatcaaaat tactgaaaaa agaaaggaag aaataaattt gaagaaaaca
      121 aattcttcag gaaaaccgaa acgcgacttc actaaattat cgacacccca tacgtatgtc
      181 attatttttg gtgtagtcat ctttgcatgg atactgactt ttgtcgttcc agcaggaaag
      241 ttcagcacgc aagatattga atacaaagat gcaaatggcg aaacaagtac tcgtactgtt
      301 ttacgtcaag attccttccg ttatgcttac gagctggata agtcgtatgt gtttgatcaa
      361 ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac tggatgttcc ggaaaaagga
      421 ttggaaaaag tcattgctga cggagaaaaa aatcttacgc aggaaaaact agatgaaatc
      481 tccttgacag atgatgtgct ttacgatcag tacggcgaaa atatctatga tacttctaaa
      541 aaattgcata aaacagccaa aatatgggga acagatgatt ttggcggctt tggtttctta
      601 aattttgttt ttgaaggact agtatctgga gataagtatg gatctgctgt agggatcgct
      661 gcattgatcc ttgttgttgg aggtgctttt ggtattatta tgcgtaccgg agcgatcgac
      721 gcaggtatct atgcgtttat cagtaaaact aagggactgg agcgtttagc tttaccgcta
      781 ttattctttg ctttttcatt cggtggcgct acattcggga tggcagaaga ggtaattcca
      841 ttctcaatgg tgatggttcc atttgtcatt gcgttaggat atgactcgat cgtagcagta
      901 accgtgactt atgtcgcatc tcaagtcgga aatgcgacct cttggatgag cccgtttagc
      961 gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt ctggtgcgac attccgattg
     1021 attatgtggg tagttgttac tgcattagcc gctggttata tgatgatata tgcagaaaaa
     1081 atccgtaaaa aaccagaaag ttcgttgact tacaaatcag atgattattt ccgttctcat
     1141 atcaaaaaga catctgatga aaataaaaaa ttcatgcttg ggcataaatt gatcttatta
     1201 gagatgttag ttgtccttgt ctggatcgtt tggggcgtga ctcaaaaagg ctattacatt
     1261 ccagaaatcg cttctcaatt ctttgtgatg ggattagttg ctggaatcat tgctgttctt
     1321 ttcaaattag atggaatgaa agtcaatgac atcgcttctt ctttccaatc tggtgcagct
     1381 gatttagctg gtacagcaat cgtagtaggt atggccaaag ggattcttct tgtcttggga
     1441 ggatctgatg catctgttcc ttcagctttg aatacgattt tgcatgggat cgggacggcg
     1501 ttgaccggtg ttccagctgt cattggtgct tgggcgatgt acatcttcca aagtttgttc
     1561 aacttggttg ttacatcaaa ttcaggacaa gcagctttga ctatgccaat catggctcca
     1621 ctggcagatt tagtaggtgt atccagacaa gtagcagttc ttgcttatca gcttggttca
     1681 ggttttatgg acgcttttac acccgtatca gccagcttga ttggtgtgct aggtgttgca
     1741 cggatcgaat gggcaaaatg ggcaagattc caaatcaaga tgcaaggatt cctgtttgtt
     1801 ttaggaacta ttttcatcat gattgccatt gcaataggtc tgcagtagta ctggagacct
     1861 gtaaataaaa aagtaatcca aacagtagat aaggactcca agaaccgtca ttttacggta
     1921 aaatcaaagg agtaaagatc ctatcgtttg gattattttt taagatccta tcatcagtta
     1981 tacttaaaga aagaggaaaa atgtatgaaa atcattcgac aaattgaagt gtttgcacca
     2041 gattacttag gaaaaatgga tgtactgatt gccggtggga agattttggc tgtagaagaa
     2101 cagctagaag gcggctacga aggagtggaa gtagaagaac tttcaggtga gggaaaagta
     2161 ctaaccccgg gtttcattga ctgtcatttc catattttag gtggaggagg agaaggcggt
     2221 tatcaaaatc gaacgccaga agtcacattg agccaattga ctacagctgg tgtaacgaca
     2281 gttgtcggtt gtttgggaac agacggagaa ggtagagata tgactgcgtt gatcagcaaa
     2341 gcgaaaggtc tggaagcaga aggaatctcc acttatgtat acgaaggttc gtaccgattg
     2401 ccggtaaaaa cagtaacaga ttctatcatc aaagactttt tgacaattga taaaattatc
     2461 ggaatcggag aaatcgcagt atccgatcac cgttcttctc agccaagttt tgaagagttc
     2521 gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat ccggaaaagc tgggatcatc
     2581 aatgtccact taggcggagg aaaaagaaag ctagagctat tgacacgaat cgtagaagaa
     2641 acagaaattc cgatcacaca atttttgcct actcatgcaa atcgcacacc agaattattt
     2701 gaagcttgcg tggcatttgc caaacgtggt ggcacgattg acttcactgc tagcgaagat
     2761 cctgattttt gggagaagac agatggtgaa gttcgcttca gcaaagcact gaaacgattg
     2821 atcgaggaag atgtttcttt agataatttc acgatgacct cagatggaca aggaagcttg
     2881 ccttattttg atgaaaacaa tcatttccta gggttaggag taggaagtgc caaggcattg
     2941 ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc ctttggaaat cgccttacga
     3001 gctataacca gtaatccagc acgtatattg aaattggata aaaaaggaaa aatcgaaatt
     3061 ggtgctgatg cagatctgtg tatcttggat aaagaaacgc ttgatattga tacagttatc
     3121 gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag tatgggggac tttcgaaaaa
     3181 agtttttaat caacagctct aactatcttt tttagaaaac gagcgttctg tttgtctgta
     3241 tctgttgttg atgttaccct gaaggtagat caataaacaa actaagagga ggaagcacaa
     3301 tggcagattt aaaaggacgt tctaaagata tgaaagataa agtagtcggt gaaggaaaag
     3361 aagcatacgg caaagcaaca gacgacaaga gcaaagaaac agaagggaaa gcacaatcac
     3421 taggtgctga catcaaagaa aaagctcgaa atttaggcga cgaagtcaaa gacaagtttt
     3481 ctcgaaaaga cgaaaaaaaa taaaaaccac ttattactaa ttgaatcaag caaataaaaa
     3541 atccgaacca tatctgcgag acagatatgg ttcggatttt ttatttgatc attatcctgt
     3601 tttttaaact tcgatttttt gaaatttttt attttttagt tcaaagactt catcacagct
     3661 ttttgctact tcaatctcat gagtcacgat gataatacag cgattttgtt catgggcgat
     3721 ttcttggaaa agtttgacga tatcttgtga agttgtttca tctagatttc cagtaggctc
     3781 atccgctacg attagttgat gttcacagca gatagcacga acgatagcga ctcgctgctg
     3841 ctgacctccc gaaagtttgg tcacctgttt ttttgctaat atttcatcaa ttcctacacg
     3901 agccagtcga tctaaagcaa cttgtcgata attctcttgc ttggtcttag tgatttccat
     3961 tgctgctatg acgttgtcta gagccgataa ataaggaagc aagttgtaag cttggaaaat
     4021 aatcgagaca tcatttttac gataacttct taacccgatt ttgttcagtg aacgatcttg
     4081 atagtatacc tttccttctt ttggtgcatc tagtccagaa ataagcgaaa gaaaagtcgt
     4141 ttttccagaa ccacttgctc ctaaaatagc gtacattttt ccagcttcga aattcagttc
     4201 tacatcttta taaagcgcat cttgcggatc ttgataccaa aatcccaatt gttctgtccg
     4261 taacatcaaa ttcctccttt agtttaatag gatttttctt gggtttaatc ttaatatccc
     4321 tatagaagct aaaaagacag aaaacagact gattccaaat gctactccgg ctaatagtcc
     4381 caattgtgca ggtttgacat tgatttccaa ttcgctgact tgatcagaaa cagcaaatgg
     4441 attattcgtg gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc tgttacctgg
     4501 catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct gttggtcaag
     4561 tagctgctgt cccacggcat tcgcgacgac gtttccgcta agcgaagcaa ctcctaatgc
     4621 aagaatcata caaatagcca cttcggtaaa caattggaga atgattttac ttctggattc
     4681 tcctaaagat agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa tcataatgat
     4741 cagagtaagt atgataatac cggctacagc cactagtaaa acaacatttt tagaaaaact
     4801 tgccacgttg tttaaaggtt ccaacattga ctgatacatg gaatcatttg attgtaagct
     4861 aaatgtatct gtgtcgatca gcttctcagc ttctttgaga aactcatcca ttttatctgg
     4921 atcagataga gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc catttagttc
     4981 atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt tgaatttcat
     5041 acccatactg ccgcttgttt cgctggattc ataaattcct ttgatcgtca tttcataagt
     5101 ggtatcttca tcttcagtac tcgtgattgt gaaagtatct ccgactgcca ggtcatttgt
     5161 ttctgctaga gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt catctattcc
     5221 ttcaccatcc gtgatttttg ctgttccatc agaaaaatta ctattttgtg cagttgctga
     5281 cacaccactg atttgaaagt ctgcttgagt catttgcgga ttcccgccca ttcctccagc
     5341 aggttcaccg cctgcatcag tagtttctga actgtcttca cttgaatcag aactagtgat
     5401 ggcagttatt ccatcttttg ccatagctga ggcggaactt tcaaaggaat aactctttac
     5461 attatctaat tcagctattt tttccgcgtc cgttaaggaa actggtgtaa gagaaaaact
     5521 gcctggatct ggacgttctt cagtggtaga accgcttgaa gaagtttgtt cctgtttttt
     5581 aaatgaagct tctcgattgg cacttaaagt cactgttgct ccaacttctt tttgtgcttg
     5641 attcgctgct tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa aaataagaat
     5701 ggctgtgaac acggcaaaca ataacaatga acgtccccat tttacttttg tacttttcca
     5761 tgcacgtttc ataaaattca tattttgcat ccctcctagt agatgcattt gataatagac
     5821 aacctatctt aaagctagct taaatcttat gctgtttgca caccatcaac atgaggttct
     5881 gtatatataa aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa aaaagtatgt
     5941 ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag atgctacaga
     6001 gggattttta cgtgcatagt tgttctaaca attgctgata ctagcgattt agtttatttg
     6061 ttgtataata atggggaaca gctgaatact tatataagta ttctggctat agcgtttcgc
     6121 aaaagatcca ttttgttaat gaagtatatc caaaataaaa tgcccatcca actgtcagaa
     6181 cgcggatgtg ctggtaattt tcctgctgat tctccataag ttgagaaata gtgattttgc
     6241 atatgcttca ttggaatggc tcatatattt agataaagaa gaaaatcgac atctttaaac
     6301 ttggatgtca taagaagagg tgaagtttgt gagtaccatt aaaattatcc cattaggcgg
     6361 cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata tttttatttt
     6421 agattgtggg ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg taatccctga
     6481 ttttacgtat ttagaagaaa acattgatcg tgtagctggt gtctttttaa cacatggaca
     6541 tgcagatgcg atcggggcac ttccttactt gttgtcaaaa atttctgtac cagtattcgg
     6601 gacagagttg acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag cagccaaagg
     6661 atttaaggat ttccatgtgg tagatgagca tacagaaata gactttggtg aaacagtagt
     6721 gagctttttc cgtaccactc atacgatacc agattcagtg gggatcaatc tgaaaacaaa
     6781 agaaggtagt atcgtttata cgggagattt taaatttgac caaacggcta tcccaatgta
     6841 tcaaacagat tatgcacgat tggcggaaat cggcaaagaa ggagtcttag ccttactaag
     6901 tgaatcggca aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa tagcagatga
     6961 agtatttgat acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg tagcaagtaa
     7021 tttgcaacga gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa aagttgtctt
     7081 gactggtcaa gatttcgaac gaattattcg cacagcgatg cgtttagaaa aacttcaatt
     7141 accaagtgaa gatctgcttg ttacgttgaa agacatgaaa aactatgatc cagaagaact
     7201 gatcatttta gaaactggcc gaatgggtga acccatcaag tctctgcaaa aaatggcaaa
     7261 cggtacacac cgtaatgtca agattgaaga aggagatttg gtttatatca ctactacgcc
     7321 aagcatcgcg atggaaacca tcgtggcgaa aacggaagat atcatttatc gagctggtgg
     7381 aacagtgaag ttgatctctg agaatatgcg cgtttctggc catgccaatc ctaatgactt
     7441 gcaattaatg atcaatttga tgaaaccaac atattttgtg ccagttcaag gagagtatcg
     7501 tgaattagct gctcatgcag atttggctca tgcagtaggg atgccataca aaaatatcta
     7561 tattactgga cgcggagacg tgctggaata taagaacaag cgtatgagtg ttgcaggaac
     7621 tacaagtgca gaaaatgtaa tgattgatgg attaggcata ggtgatatcg gaaacattgt
     7681 tttacgtgat cgaaaaatct tgtcagaaga cgggattttc gtagcggttg tcactatcag
     7741 tcggcgagaa aagaaaatca tttcaaaacc tcaaattact tcacggggat tcgtttacgt
     7801 aaaagctagc cgtgatctga tgaaagaaag cagcaatatg attgaaacaa tcgttgagaa
     7861 acatttagaa agcaatgatt ttgagtggag caaattaaag caagacatcc gagatcaatt
     7921 aggccgttac ttatttgaac aaacaaaacg ccgcccagtg atcttgccag tcattatgga
     7981 agctactcaa cgtcgaaata aaaaataaac aaaaagagta ag
//
LOCUS       DADXTH010000103         7628 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.105, whole genome shotgun sequence.
ACCESSION   DADXTH010000103 DADXTH010000000
VERSION     DADXTH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.105"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWI97_002496"
     CDS             complement(14..706)
                     /locus_tag="IWI97_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBC2463969.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="IWI97_002497"
     CDS             complement(1143..1712)
                     /locus_tag="IWI97_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBC2463970.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWI97_002498"
     CDS             complement(1786..2109)
                     /locus_tag="IWI97_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463971.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWI97_002499"
     CDS             complement(2263..3075)
                     /locus_tag="IWI97_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463972.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWI97_002500"
     CDS             3429..4277
                     /locus_tag="IWI97_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBC2463973.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IWI97_002501"
     CDS             4422..5363
                     /locus_tag="IWI97_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBC2463974.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWI97_002502"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWI97_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2473 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttt
//
LOCUS       DADXTH010000104         7599 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.106, whole genome shotgun sequence.
ACCESSION   DADXTH010000104 DADXTH010000000
VERSION     DADXTH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.106"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(941..1354)
                     /locus_tag="IWI97_002503"
     CDS             complement(941..1354)
                     /locus_tag="IWI97_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBC2463975.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="IWI97_002504"
     CDS             complement(1810..2295)
                     /locus_tag="IWI97_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463976.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="IWI97_002505"
     CDS             complement(2434..2649)
                     /locus_tag="IWI97_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBC2463977.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IWI97_002506"
     CDS             2873..3673
                     /locus_tag="IWI97_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2463978.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IWI97_002507"
     CDS             3692..5560
                     /locus_tag="IWI97_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBC2463979.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IWI97_002508"
     CDS             5553..6044
                     /locus_tag="IWI97_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBC2463980.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IWI97_002509"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IWI97_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBC2463981.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="IWI97_002510"
     CDS             6604..7599
                     /locus_tag="IWI97_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBC2463982.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DADXTH010000105         7350 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.107, whole genome shotgun sequence.
ACCESSION   DADXTH010000105 DADXTH010000000
VERSION     DADXTH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.107"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWI97_002511"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWI97_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBC2463983.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3191)
                     /locus_tag="IWI97_002512"
     CDS             complement(2886..3191)
                     /locus_tag="IWI97_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2463984.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3336..4541)
                     /locus_tag="IWI97_002513"
     CDS             complement(3336..4541)
                     /locus_tag="IWI97_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBC2463985.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5235..5621
                     /locus_tag="IWI97_002514"
     CDS             5235..5621
                     /locus_tag="IWI97_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBC2463986.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5719..5910
                     /locus_tag="IWI97_002515"
     CDS             5719..5910
                     /locus_tag="IWI97_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2463987.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6424..6756
                     /locus_tag="IWI97_002516"
     CDS             6424..6756
                     /locus_tag="IWI97_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2463988.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6666..6869
                     /locus_tag="IWI97_002517"
     CDS             6666..6869
                     /locus_tag="IWI97_002517"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBC2463989.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            7053..7208
                     /locus_tag="IWI97_002518"
     CDS             7053..7208
                     /locus_tag="IWI97_002518"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBC2463990.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2463 a         1298 c         1046 g         2543 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagta tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctaaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcaataagtt tggtaagatc attatcagta attgaaccta
     1141 caccataatt ttcatgatct ttgcgccgtt caattgctaa atcagccact tttttccctg
     1201 attcaaaaga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagctga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggttcc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta agctcttttc aaacgtaatt accccattag
     1681 gagcagtttg tgtatcagtt gtttcagccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggg
     2101 tgcttggaga aatttcatct tgtgcttgaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaaccaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa tagttatttt cttagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa acttatgtta actagtttac ttaaagaatt tcatgcacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ctctttctta gattaaactt cagagtattc
     2581 aaatagcgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcattcctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt attaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgcttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3301 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattatcc
     3361 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3421 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3481 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3541 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3601 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3661 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3721 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3781 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3841 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3901 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     3961 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4021 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagatttcct
     4081 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4141 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4201 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4261 aaatctttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4321 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4381 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4441 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4501 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4561 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4621 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4681 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4741 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4801 aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt tcgttcaaat
     4861 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4921 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     4981 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5041 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5101 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5161 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5221 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5281 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5341 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5401 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5461 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttaata
     5521 aaactgataa agaaattgga aaggttctgg gtattacaag acagggtgcg acaaatttaa
     5581 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5641 tttgtatgct tctttgctcg tcctcctcct tcccaaaaaa ataaatagat tataagttca
     5701 tgggaagaag gtaaatttat gaatacttta aaagaagatt tcattttagc aaagaatgga
     5761 aatgaagaag cagttgtggc cattctcaaa agatttagcg ctttggtcca taagcaatcc
     5821 tggagaactg gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta
     5881 gctatctcga aattcgaaat taaagaataa ctcaactaag ggagtctgtg acataagtaa
     5941 aaagatagtt tcaaatttcg aacaataaat ttgaagccat cttctttata attataaagt
     6001 atcgatttta cagcgttcta taatgattca tagaatctaa aacgaatgat tttaattatt
     6061 aacgtttttt aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa
     6121 ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc agaactgggc catggtactt
     6181 caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta
     6241 gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat
     6301 tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac
     6361 taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact
     6421 ctattgaaaa aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag
     6481 aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc
     6541 cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga
     6601 ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa
     6661 gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt
     6721 acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt
     6781 tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag
     6841 aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa ccaaattgat gctgtttggg
     6901 tgacggatat tacttacatt ccatgtaatg acggtcgcct ctatttgtcc acctatattg
     6961 acttagcaac tcgtattcta cgttactata tggtggattc acacatgaaa aaagagattg
     7021 tcgtccgtcc attacaaatt tattaaggag aattgcctac ggtaattcat tccgatagag
     7081 gtagccagca tcggtctttt atttatcaag agctgttgac cgaccaccac atcacacatt
     7141 caatgagcca aactgggact cctgttgaca atgcagtaat tgaagcgttt catcgatcaa
     7201 tcaaatgaga attaatcgac tccaacaaat ataaaagtaa aatagagatg aaggtgttaa
     7261 tccaagatta tttagaagac tactacccaa acaaaagaat ccatacaaaa tttatgatga
     7321 ctccttgaca atttgaagcg aagaaaataa
//
LOCUS       DADXTH010000106         7055 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.108, whole genome shotgun sequence.
ACCESSION   DADXTH010000106 DADXTH010000000
VERSION     DADXTH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.108"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IWI97_002519"
     CDS             complement(<1..142)
                     /locus_tag="IWI97_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2463991.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IWI97_002520"
     CDS             complement(183..437)
                     /locus_tag="IWI97_002520"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBC2463992.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IWI97_002521"
     CDS             complement(548..802)
                     /locus_tag="IWI97_002521"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2463993.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            1413..1868
                     /gene="tcrY"
                     /locus_tag="IWI97_002522"
     CDS             1413..1868
                     /gene="tcrY"
                     /locus_tag="IWI97_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-responsive transcriptional repressor
                     TcrY"
                     /protein_id="HBC2463994.1"
                     /translation="MSTIAVDLHITDAEWEVMRVVWANGRVTSKEVISTLGEKMDWKQ
                     ATTKTLLGRLVEKGALNTEQEGRKYIYSANIEEKEAVRSFTNDIFDRICRKNVGNVVG
                     SIIEDHVLSFDDIQRLEEILEMKKAFAVEEVDCQCTEGQCECHLHHHGE"
     gene            1881..4346
                     /locus_tag="IWI97_002523"
     CDS             1881..4346
                     /locus_tag="IWI97_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBC2463995.1"
                     /translation="MENKSFAIEGMTCASCAQTVEKAAKKVRGVTQASVNLATEKLSI
                     EYDEPTFSVENLQKAVDNSGYELIAQEGTTQTFAIEGMTCASCAQTIEKAVGKLSGVD
                     KASVNLATEKMQVSYNPSAISVSDVTGAVSNSGYAAVLETTETQDNSRAEKREKKEKR
                     MKQLSNRFGISIIFTIPLLIISMGPMVGMPLPNIVDPMINAFNFSLLQLILTLPIMVV
                     SWEYFQKGFKTLFKGHPNMDSLIALGTAAAFVYSLAATIGAGLGYGNFSDLLYYEVTG
                     VILTLHTLGLFLEERSKGQMSSAIEKLVNLVPKTARVIRNGVEQEITVDEVALGDVIR
                     VRPGESMPVDGVVVEGRTSVDESMLTGESIPVEKESGDEVIGASINKNGSIDYRATRV
                     GSDTTLSQIIKLVEDAQGSKAPIARMADIITRYFVPIVIALAVLAGIAWLIAGQSGIF
                     ILSVIITTLVIACPCALGLATPTSIMVGTGKGAEHGVLIKSGEALETTHNLDTIVFDK
                     TGTLTEGKPIVTDILVTPLITKENLLYYAASGETGSEHPLGEAIVQKSKEENMTLAKP
                     DHFEAIPGHGIRVEIEGKDMYIGNRKLMLEQKIDLSSMEKESNRLADEGKTPMYLSVD
                     GELAGIIAVADTLKENSMKAVKELRRRGVEVIMITGDNKRTAKAIAKQVGIDSVLSEV
                     LPEDKAEEVKKLQEAGKKVAMVGDGINDAPALAQADIGIAVGSGTDVAIESADIVLMR
                     NDLTAVLTAIDLSHATLRNIKQNLFWAFAYNLVGIPVAMGLLYIFGGPLMNPMFAAVA
                     MSFSSVSVLLNALRLRRFKPAVV"
     gene            4431..4637
                     /gene="tcrZ"
                     /locus_tag="IWI97_002524"
     CDS             4431..4637
                     /gene="tcrZ"
                     /locus_tag="IWI97_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone TcrZ"
                     /protein_id="HBC2463996.1"
                     /translation="MKKEVLLDGVKCAGCANTVQEKFSAIEGVESVEVDLATKKAVLE
                     SQTEIDTETLNAALAETNYSVLSV"
     gene            4776..6908
                     /gene="tcrB"
                     /locus_tag="IWI97_002525"
     CDS             4776..6908
                     /gene="tcrB"
                     /locus_tag="IWI97_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase TcrB"
                     /protein_id="HBC2463997.1"
                     /translation="MRNNKKHSSHSHHNHGDMDHSKHDHNEMEHSQMDHSKMNHSAMD
                     HSKMNHSQMDHSEMDHGAMGGHAHHHHGSFKEIFLKSLPLGIAILLITPMMDIQLPFQ
                     IIFPYADVVAAVLATILYIYGGKPFYMGAKDEFNSKAPGMMSLITLGITVSYAYSVYA
                     VAARYVTGEHVMDFFFEFATLILIMLLGHWIEMKALGEAGDAQKALAELVPKDAHVVL
                     EDDSIETRPVSELQVGDVIRVQAGENVPADGIIIRGESRVNEALVTGESKPIEKNPGD
                     EVIGGSTNGGGVLYVEIKQTGDKSFISQVQALISQAQGQSSRAENLAQKVAGWLFYIA
                     VIVALIALVIWMIIADVPTAVIFTVTTLVIACPHALGLAIPLVTARSTSLGASRGLLV
                     KDRDALELTTNADVMVLDKTGTLTTGEFKVLDVELFNDKYTKDEIVALLSGIEGGSSH
                     PIAQSIISYAEQQGIRPVSFDSIDVISGAGVEGQANGHRYQLISQKAYRRNLDMDIPK
                     GATLSVLVENDEAIGAVALGDELKPTSKDLIQALKKNKIQPIMATGDNEKAAQGTAEI
                     LGIDYLANQSPQDKYELVEKLKAEGKKVIMVGDGVNDAPSLALADVGIAVGAGTQVAL
                     DSADVILTQSDPGDIESFIELAQKTTRKMKENLVWGAGYNFIAIPIAAGILAPIGITL
                     SPAVGAVLMSLSTVIVAINAMTLKLDPK"
BASE COUNT         2258 a         1216 c         1541 g         2040 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc ataccagaac ttacattcga
      901 gttatagtat agacagagag tagcatatca ttctcctgga ttttaagatg gtactcccca
      961 atgagctact catacaatta tagtccgata aagacgcaaa aaggcagggc tctcaccttg
     1021 ctttttttgt gtttaaagct gaaatagttt gtagatgaat aatttgcgat gctgtgaccc
     1081 cttgagcctg ttagggtcaa atttgtaatc aaaatgaaat cggaggatag aaaacccgta
     1141 cagtgggatg tctatccacc gacattctga aaaagcttat tttcggtgtt tttttaaaag
     1201 agaagtgtcc gacgaaccaa tccctaatta atgaactaaa acacaatcca tatttttgtc
     1261 aggcgtgtcg catcgaaacg aaagttaaaa agttataaaa aataatacat cacaaattat
     1321 tcatttacat aaactcttga caataatcgt ttactagtgt aaactctatt tagcattaat
     1381 gcttacgcgc gtaaacgaaa gggggagaga atatgagtac tatagctgta gaccttcata
     1441 taaccgatgc tgaatgggaa gttatgcgtg tagtctgggc gaatggtcga gtgactagta
     1501 aagaagtcat ttctacattg ggagagaaaa tggactggaa acaagcgaca accaaaacgc
     1561 tcttaggtcg actcgtggaa aaaggcgcac tgaatacaga acaagaaggt agaaaatata
     1621 tttattcggc aaatatcgaa gagaaagaag cggtaagaag ttttacaaac gatattttcg
     1681 atcgtatttg tcgaaagaat gtcgggaatg tagtagggag tatcattgaa gatcatgtct
     1741 taagcttcga tgatatccag cgactagaag aaatattaga gatgaaaaaa gctttcgcag
     1801 tagaagaagt ggattgtcag tgtacagaag ggcaatgcga gtgccattta catcatcatg
     1861 gagaataagg agcgagaaaa ttggagaata aatcgtttgc catagaaggt atgacctgtg
     1921 cgtcttgtgc gcagacagta gaaaaagcgg caaaaaaagt acgtggggtc acacaggctt
     1981 ccgtgaacct agcaacagag aagttaagta tagagtacga tgaaccaact ttttcggtag
     2041 aaaatctaca aaaagcggtg gataattcag ggtatgaact gattgcccaa gaaggaacga
     2101 cgcaaacctt tgcaattgaa gggatgacct gtgcatcctg tgcacagaca attgaaaaag
     2161 ctgtcggaaa gttgtcgggc gtagataaag cttcagtcaa tttagcgaca gaaaaaatgc
     2221 aagtttctta taacccgtca gcaatttcag tatctgatgt gactggtgct gtatccaata
     2281 gtggttatgc agccgtatta gaaacaactg agactcaaga taattcacga gcagagaagc
     2341 gtgagaaaaa agaaaaaaga atgaagcaac tttcaaatcg ttttgggata tccattatat
     2401 ttacaatccc tttactaatt atatccatgg gtccaatggt cggtatgcct ctaccaaata
     2461 tcgttgaccc aatgataaat gcatttaatt tttctctttt acaacttatt ctgactttgc
     2521 cgataatggt agtaagctgg gaatatttcc agaagggatt caaaacctta tttaaaggtc
     2581 acccaaatat ggattccctg attgcccttg gtaccgcggc cgcttttgta tacagtctgg
     2641 cagcgacgat cggggctggc ttaggttacg ggaacttttc agatttactt tactatgaag
     2701 tgactggagt tatcttaact cttcatacgt tagggttatt tttagaagag cgatcaaagg
     2761 ggcaaatgtc ctcagcgatt gaaaaattag tcaacttggt tcctaaaaca gctcgagtga
     2821 tacgtaatgg tgtggaacaa gagattactg tggacgaagt cgctttagga gatgttattc
     2881 gagttcgtcc gggagaaagt atgcctgtag atggtgttgt tgttgaggga cgcacttcag
     2941 tcgatgaatc aatgctgaca ggagaaagta tccccgtgga aaaggaaagt ggagacgagg
     3001 ttattggggc tagtatcaat aaaaatggtt ccattgatta ccgcgcgact cgagtgggaa
     3061 gtgatacaac tctatctcaa atcataaaat tagtagaaga tgctcaaggg tctaaagcac
     3121 ccattgctcg aatggccgat atcattacca gatatttcgt accgattgtt atagcattag
     3181 ccgttttagc agggattgct tggttaattg caggtcaatc tggaatattt attttatccg
     3241 ttatcataac aacccttgtc attgcttgtc catgtgcctt aggtttggcg actccaacaa
     3301 gtattatggt tggaaccgga aaaggggcag agcatggtgt cttgataaaa agtggtgagg
     3361 cgttagaaac cactcataat ttagacacga ttgtatttga taaaacaggg acactgacag
     3421 aaggtaaacc tatcgtgacg gatattttgg taactcctct tattactaaa gaaaatcttt
     3481 tatattatgc agcttctggt gaaacaggtt ctgaacaccc attaggcgaa gccatcgtac
     3541 aaaaatcaaa agaagagaac atgacattag ctaaaccaga tcattttgaa gcgattcctg
     3601 gacacgggat tcgagttgaa attgagggca aagatatgta cattggaaac cgtaaactga
     3661 tgctagagca aaagattgat ttatcaagca tggaaaaaga atccaatcgt ttagcagacg
     3721 aaggaaaaac accgatgtat ctctctgttg atggagaact agctggaatt atcgcagtag
     3781 ctgacacact caaggaaaat agtatgaagg ctgttaaaga acttagaaga cgcggtgttg
     3841 aagtaatcat gattactgga gataacaaac gtacagccaa agcgattgct aagcaagtgg
     3901 gtatagacag tgtattaagt gaagtattac ctgaagataa agcagaggaa gtcaaaaaac
     3961 tacaagaggc aggcaagaag gtagcgatgg ttggagacgg cattaacgat gcacccgcat
     4021 tagctcaagc agatatcggt attgcagttg gatcaggtac agacgtggcg attgaatcgg
     4081 ctgatattgt ccttatgcgt aatgatttaa ccgctgtatt gactgcgatt gatttaagtc
     4141 atgcaacgct acgaaacatt aaacaaaact tgttctgggc ttttgcttac aaccttgtag
     4201 gtattccagt cgcgatgggt ctcttgtata tttttggcgg accattgatg aatccaatgt
     4261 ttgcggcagt cgccatgagt ttcagctccg tatcggtctt gctcaatgct ttacggttaa
     4321 gacgattcaa accagcagtt gtttagaaaa cacgtagctt atctgggatt taaacttctt
     4381 cctattctaa taataggaga agataaaaaa ataaaaaggg gaatagaata atgaaaaaag
     4441 aagtcttatt agatggcgta aaatgtgcag gttgtgccaa tacagtgcaa gaaaaatttt
     4501 cagctattga aggggttgaa tctgtagagg ttgatttagc gactaaaaaa gcagtacttg
     4561 aaagtcaaac agagattgat accgaaacgc tcaatgctgc tttagcagaa actaactatt
     4621 cagtattaag tgtataaagt acgaatacca tttattcata gatagtagat agagataaaa
     4681 tatagaaccc ttttcatgag aattatatgc agtgttagat gagaaagttc taaaatttta
     4741 aaatcagtct ctaatataat aaggaggaat taaaaatgag gaataacaaa aagcattcgt
     4801 cacatagtca tcataatcac ggcgacatgg atcactcaaa acacgaccat aatgaaatgg
     4861 aacatagtca gatggatcac agcaagatga atcatagtgc gatggatcac agcaagatga
     4921 atcatagtca gatggatcac agtgaaatgg atcatggcgc aatgggaggg catgcccacc
     4981 accatcacgg tagctttaag gagattttct tgaagtcact cccattagga attgcaatct
     5041 tactcattac tcctatgatg gatattcagt tgcctttcca aatcatcttt ccttatgcag
     5101 acgttgtagc agctgtattg gcaacaattc tatacattta tggcggaaaa ccattctaca
     5161 tgggtgcgaa agatgagttt aattcaaaag ctccaggcat gatgtccttg attactttgg
     5221 gaataacggt ttcttatgcc tatagtgttt acgcagtggc cgctcgatat gtaactggag
     5281 aacatgtcat ggacttcttc tttgagtttg caacgttaat tttaatcatg ttattaggac
     5341 actggattga aatgaaggca ttgggtgaag caggggacgc gcaaaaagca ttggctgagt
     5401 tggtgccgaa agatgctcat gttgttttag aagacgattc gattgagacg cgccctgtgt
     5461 ctgaacttca ggttggagat gttatccgtg ttcaagcagg agaaaatgtt ccagctgatg
     5521 gtatcattat tcgcggagaa tcccgtgtaa acgaagcgct tgtgactggg gaatcgaaac
     5581 caattgaaaa aaatcccgga gatgaggtca tcggtggatc aaccaatggt ggcggagtcc
     5641 tctatgtgga aataaagcag acgggcgata agtccttcat ttctcaggtt caggcattaa
     5701 tcagtcaagc tcaaggccaa tcttctcgag cagaaaatct tgcacaaaaa gttgcaggat
     5761 ggctctttta tattgcggtt attgtagcgc ttattgccct tgttatatgg atgattatcg
     5821 ctgatgtacc aacggcagtt atttttacag tcaccacatt agttattgct tgcccgcatg
     5881 ccctgggtct ggctattcca ttggtgactg ctcgtagtac cagcttaggg gccagtcggg
     5941 gattattagt gaaagaccgg gacgctttgg agttgactac aaacgcagat gttatggttt
     6001 tagataaaac tggaacttta acaactggtg agtttaaagt attagatgtt gaactcttta
     6061 atgataaata tacgaaagat gaaatcgttg ccttattgtc aggtatcgaa ggtggatcta
     6121 gtcaccctat cgctcagtca attattagtt acgcagaaca gcaagggata cgtccagtaa
     6181 gttttgattc gattgatgtt atttctggcg ctggtgtaga aggtcaagct aacggacatc
     6241 gttatcaatt aatcagtcaa aaagcatata gacgtaatct ggatatggat attccaaaag
     6301 gagcaaccct cagtgtctta gtagaaaacg acgaagcaat cggtgctgta gctttagggg
     6361 atgaattaaa accgacaagt aaagacttaa tacaagccct taaaaagaac aagattcagc
     6421 cgattatggc aacgggtgac aatgaaaaag cggctcaagg aactgcagaa attttaggta
     6481 ttgattatct agctaatcaa tctcctcaag ataaatatga attagttgaa aaacttaaag
     6541 cagaaggaaa gaaagttatc atggtcggtg acggggtcaa cgatgcacct tctcttgcat
     6601 tagcagacgt tggtatagct gttggtgccg gaactcaagt agcattggat tcagccgatg
     6661 tcatcttgac acagtcagac cctggcgata ttgaatcctt tatcgagttg gctcagaaga
     6721 ccacacgtaa gatgaaagaa aatcttgttt ggggagcagg gtataacttt atcgcgatcc
     6781 caatagctgc aggaatacta gcaccaattg gcataacatt aagtccagca gtcggtgccg
     6841 tcttgatgtc actttcgact gtaattgttg ccatcaatgc tatgacattg aaattagatc
     6901 caaaataaac agttatttgg cacatgaaat aattgaatga ccttatcgtg taaaaataca
     6961 atagtttcac aggaaaggaa gagatgaaat gatttaaaaa atcatctctt ccttttttag
     7021 tttataagga ggaaaaatta tgaggctagt ccttt
//
LOCUS       DADXTH010000107         6785 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.109, whole genome shotgun sequence.
ACCESSION   DADXTH010000107 DADXTH010000000
VERSION     DADXTH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.109"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            17..391
                     /locus_tag="IWI97_002526"
                     /pseudo
     CDS             17..391
                     /locus_tag="IWI97_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(731..1887)
                     /locus_tag="IWI97_002527"
     CDS             complement(join(731..1608,1608..1887))
                     /locus_tag="IWI97_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBC2463998.1"
                     /translation="MSQRRERRTYTQEFKQQMVDLLNSGKPRADIIREYELTPSSFDK
                     WVRQAKKTGSFKEADNLTPTEKELIALRKRNQQLEMENDILKQAALIFGPKRQVIDAN
                     KHTYSISAMCKLLNISRQTYYYKTKKKPSEAALEAAVTEEFNRSRKNYGTRKIKAALA
                     KRDILVSRRKISQIMNRRGLKSNYTKANYRKHTSNVNHSDIGNTLDRAFTEREPLEAI
                     ITDLTYVRAGTTWCYVCFIIDLFNREIIGHSCGPNKDAALVKSAFQSISHPLTEVKIF
                     HTDRGKEFDNKLIDTILETFDIERSLSKKGCPYDNAVAESTYKSFKVEFVYPNTFDTL
                     NQLKLQLFDYVNWWNYLRLHGSLGYETPIGIRNLRLAERILDNEHGCGSVA"
     gene            1974..3200
                     /locus_tag="IWI97_002528"
     CDS             1974..3200
                     /locus_tag="IWI97_002528"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019149.1,HMM:NF024800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2463999.1"
                     /translation="MGFNKIAKYLNLQGVEKVKRDNGTLSQWSTHFVRMILDNPVYCG
                     KIAFGRRTREKVKGTKNEYRQVRQDDYIIADGQHDAIIDEELWNQAHEKREITGVKSP
                     SKIGRDRAHLLSGILKCPKCGGPMYTNKHAWTNKDGTYKEVYYYVCSKARTARGKTCE
                     YKSMLKKTDIEPLVIEAIRELIKNQDFATEIKSKIGKEIDTSTLNRELKNYEAKLREV
                     DLNKSRLENEIDSLPEDTRFRERKINDMTFRLDGLYDIIIELEEKIEDVKLRRKAVEQ
                     DAITLENIYTLLANFDKVYDKINDEEKKSLISSLIKEIEIFPCDEADLPLKSILFNFP
                     VYKDGGDVCWFLWDKSTTVSGVIKCHYFIPYLLLHLTWLGLLIYLEEYICHPYPVHHN
                     MQNLHLPPHWKEYLKF"
     gene            complement(3908..5503)
                     /locus_tag="IWI97_002529"
     CDS             complement(3908..5503)
                     /locus_tag="IWI97_002529"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBC2464000.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEALS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            5627..6412
                     /locus_tag="IWI97_002530"
     CDS             5627..6412
                     /locus_tag="IWI97_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HBC2464001.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKILKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            6405..6647
                     /locus_tag="IWI97_002531"
     CDS             6405..6647
                     /locus_tag="IWI97_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464002.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         2142 a         1273 c         1219 g         2151 t
ORIGIN      
        1 tattggaggt atgactatga ataggagtgg gaaaaaatgt gtactttatc ctcgtgtaag
       61 tacagaaatg caagttgacg gttttagttt agatggtcag aaaaatagct taaaacgatt
      121 tgcagataga gaagaaatgg agattgtaaa tatttatgag gatgctggaa aatccggaaa
      181 atctatagat ggcagacctg catttaagca aatgttattc gatattgaaa acgggctgga
      241 aattgagtat atcttagttt ataagctatc tcgctttggc agaaatgcag ccgacatttt
      301 aaactcttta gaacacgttc aatcctttgg tgtaaatttg atttgcattg aagaagggat
      361 tgactcatca cagacaagcg ggaaactttt aatatctgta ttgtctgctg ttgctgaaat
      421 agagcgtgaa aacatcatag aacaaaccat gaacggacga aaagaaaaag cccgtcaagg
      481 cggttggaat ggtggttttg ccccttatgg ctactacctc aaagataagc aactttatat
      541 ccaagaaaat gaagccgaag cgatacgaat catctttgat aaatatgtaa atggaatggc
      601 aatacttttt ttgacaaaaa ttataaagtg taaaaatcag gtgcttgtta tctaatccga
      661 ttgtccttga caacgagtca ccatggacgt ggaacaaccg tggcgaagca acgattaaat
      721 gtatctgcca ctacgcaacg ctaccacatc catgctcatt atcaaggatt cgctccgcca
      781 atctcagatt tcgaatgccg attggtgtct cataccccaa agaaccatgt aaccgaagat
      841 aattccacca attgacgtaa tcaaataatt gtagtttgag ttgattcaat gtgtcaaatg
      901 tattcggata aacaaattct actttaaatg atttataggt tgattctgcc actgcattat
      961 cataaggaca gcctttcttg ctcagtgatc gttcgatgtc aaatgtctct aagatcgtat
     1021 caatcagttt attatcaaat tctttcccac gatctgtatg gaagattttg acttcagtca
     1081 aaggatggga gatactttgg aaagcacttt tgaccaacgc tgcatctttg ttcggaccac
     1141 aggaatgacc aatgatctca cgattgaata aatcaataat gaaacaaaca taacaccacg
     1201 ttgtgccagc tcttacataa gtcagatctg tgatgattgc ttccaaaggt tcacgttcag
     1261 tgaaggcacg atccaaggta ttgccaatat cggagtgatt tacattcgat gtatgctttc
     1321 gataatttgc ttttgtataa tttgatttca aacctcgtct gttcatgatt tgactgattt
     1381 tgcgacgact aactaggata tcacgttttg ccagagcagc tttgattttc cgcgtaccat
     1441 agttcttgcg gctacgattg aattcttctg ttacggcggc ttcaagcgca gcttcactcg
     1501 gctttttctt tgttttgtag taatacgttt gacgggaaat atttaataat ttacacatcg
     1561 ctgatatgga atatgtatgc ttgtttgcat caatcacttg tcttttcgtc cgaatatcag
     1621 cgctgcttgc tttaaaatat cattctccat ctcaagttgt tggtttcgtt tgcgtaaggc
     1681 tataagctct ttttccgttg gggtcagatt atcggcttct ttgaatgaac ctgtcttttt
     1741 tgcctgacgg acccatttgt caaaagacga tggtgttagt tcatattctc tgatgatgtc
     1801 ggcccttggt tttccagagt ttagtaaatc gaccatttgt tgtttaaatt cttgcgtata
     1861 tgttctgcgt tctcttcttt gcgacataac atgattcctc cagtgtgttt tcttatagtt
     1921 tacacacctt atcttttctg tctagtttat tgtagcctat ccaaaatgac aatatgggtt
     1981 ttaataaaat tgcgaaatac cttaaccttc aaggcgtaga aaaggtaaaa cgtgataacg
     2041 gtacactttc acaatggagt acacatttcg ttaggatgat tcttgacaat cctgtgtact
     2101 gtggaaaaat tgcttttggc agacgaacaa gagaaaaggt caaaggtact aaaaatgaat
     2161 atcgccaagt acgtcaagat gattacatta ttgcagatgg gcaacatgac gctatcatag
     2221 atgaagaact ttggaatcaa gcacatgaaa aacgggaaat cacaggtgta aaatctccat
     2281 ctaaaattgg tcgagataga gcccatttgt taagcggtat tctaaaatgc cctaaatgtg
     2341 gcggcccgat gtacaccaat aaacacgctt ggacaaacaa ggacggtaca tataaagagg
     2401 tttattatta tgtgtgcagc aaggctcgga cagccagagg aaaaacctgc gagtataagt
     2461 caatgctcaa aaagactgat attgagccac tcgtcattga agcaatcaga gagctaatta
     2521 aaaatcaaga ttttgcgaca gaaatcaaat caaaaatagg caaagaaatt gatacgtcta
     2581 ctttaaacag agagctaaaa aactatgaag ctaaacttcg agaagtagat ttaaataaaa
     2641 gtcgccttga gaatgaaata gacagcctac cggaagatac tcgttttaga gaacgtaaaa
     2701 ttaacgatat gacattcaga cttgatggtc tgtatgatat tatcatagaa ttagaagaaa
     2761 aaattgaaga tgtcaagcta cgtcgcaaag ctgtagagca agatgctatt acattagaaa
     2821 atatctacac cctattggca aactttgata aagtgtatga caaaatcaat gatgaagaga
     2881 aaaaatccct aatttcttcg ttaatcaaag aaatagagat tttcccatgt gacgaggctg
     2941 atttgcctct aaagtctatt ttatttaatt ttcctgttta taaagacggt ggagatgttt
     3001 gttggttttt gtgggacaaa agtactaccg tctctggtgt tataaagtgt cactacttta
     3061 ttccttacct gttgttgcac ctgacatggc taggactgct gatttatctg gaagaatata
     3121 tttgtcatcc ttatcctgtt catcataata tgcaaaatct ccacctacct ccccactgga
     3181 aagaatattt gaaattctaa caattggcac accagtgttc ctgaatttag agcttttgaa
     3241 tgcaaaaccg ccacgtaagg gagctatatc acccaactta cgctcttccc aatcttccgt
     3301 aaatcctgga aaacggattt ctggcacttt tgctccgttt ttagggaaca ttttttgtaa
     3361 gaatcctttt ttcgtttcct tcagcaaatc taacttacgt tgatgaaggg cgatagtgtt
     3421 atccagctgc ttgaagaatg aaccgatttt gttttgttcc tcaattgtag ggacacttat
     3481 tatagcttct ttaatatcag tagaattaat actttcaaat gttgatccag tgctatatct
     3541 agtccaatac ccgtcggact tcattttacc aagattctga aatataaatt cattaccttt
     3601 tatggcagca acacctcgac caataaccac gtcataagct gttttaccaa tatctccaac
     3661 cggtgctcgg acacttagaa tgagatcatc tttttcagct tgtttagtaa cttgagttgt
     3721 ccatactctc gggaaaacac gaccattttt catgtcagca ttaccttgca caagaatata
     3781 atcattagga tcatcagtat aattttctga attaggagac tgtcccatca ctattcgaac
     3841 ttcgtctccc aacttacgct cttcccaatc atccgtaaat ccaggaaacc gaatttctgg
     3901 ttgcgtatca ttgctcatgt ttgaatacct ccaacgcacc ttgcaaccac gcttcatttt
     3961 caggtgttgt ttgcaaagaa gagagtgctt ccaagagttc tttttctagc acttgctgtt
     4021 cttgtcgtat cttcttgatg tcatttccga catgaaccat atcaattggt tcttcttctt
     4081 cgaacgtatc tacatagcgt gggatattta agttatagtc attttctttg atttcatcaa
     4141 aggttgccaa atgagcgtat ttctcgacat cttttctttc agcgtaggtt tcgaggattt
     4201 tttgaatgtt ttcttccgat aatttattct gattttttcc tttgacaaat tctcgactag
     4261 cgtcaatgaa taatacatct cgagtttggc ggttcttttt caatacaatc actgtcgttg
     4321 gaatcgatgt accaaaaaac aagttcgcag gcataccgat cacggcataa atactgccgt
     4381 cttctaataa tttttgacga atgacccctt ctgcagcgcc acggaataaa actccgtgtg
     4441 gcaagacgat agccatggtc cctgtttcct tcaaatgata gaaaccatga agaagaaacg
     4501 caaaatctgc tttggatttt ggcgctaatt ttccataacg attaaaccga gaatcatcta
     4561 aaaaggtcgt atctgctgac cagttagccg aatagggtgg attcatcact actgcatcaa
     4621 atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt atccccattg cggagattca
     4681 tctcttcagc atctacccca tgtaaaatca agttcatttt tgctagattg tatgtcgttg
     4741 tatttaattc ttgcccatga tatttaacgt tatctgggtg agttaagtag ttacgtacgt
     4801 ttaacatcaa tgagcctgaa cccatcgttg gatcaaaaac actaaagaat cgtcgttctt
     4861 tttgatccaa cgtcacgatt tgtgccatca tatcagaaac catatgaggc gtgtaaaatt
     4921 ctccggcttt cttccctgct tctgaagcaa attgactgat cagaaattca tacgcatccc
     4981 cgatcacgtc cccatcgtga cctaaaactt ccacgtcatt caatttctta atgacttcgg
     5041 taatcgtcac gtttcgttgt tgctcatctg ttcctagttt ttttgattgg agatccacat
     5101 catcaaataa cccattaaat tgattgtagg tagatgccaa ttgcacaaag gctttgttta
     5161 gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc taagacgttg aaaagatact
     5221 ctggcgcaat cgcatacccc aaaatatcaa cgattgtggc aatcaggtct tcttttgatt
     5281 cttcatctga aagtaattca cgatacaaca tcgtttgttt gcttaccgta tcatattcct
     5341 ctaaggattc atctgctaaa agaaccactt gttccaacaa tcggtcggat aaatatttat
     5401 agaaaatcag ccccaataaa taatttttat attctgacgc atccattttg ctacggagat
     5461 tatctgccgc actaaataat ttttgattta attctgcacc caaaaagata cactcctatc
     5521 tcttatttaa agttgctata taccctctta ttttagcata ctccctttta gaaaaagcaa
     5581 aaaaaaagaa agaatcttat tcttgataac aagctacgtg acgtatatgc ctaacaaaaa
     5641 tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat ccattatttt
     5701 tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaattttaa aagaactttt
     5761 ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac ctgatagtga
     5821 taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat acatcaggga
     5881 taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag atgctgcttt
     5941 aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga atgactactt
     6001 gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt tagaggaata
     6061 tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg tatatcgagc
     6121 tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa ataaatacaa
     6181 tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa aatggaatcg
     6241 tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt ggaaagcatt
     6301 aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa atagttattt
     6361 agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact aacaatatcc
     6421 ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa aactttgaat
     6481 ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac gaaacaatcc
     6541 ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa cttaagaaaa
     6601 atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag ggaaaatatg
     6661 cttacgaaat tatccaccta gaagaaatac ttttaccaat aagtctgtct aatttaaaaa
     6721 atttaggagt tactgcacca caaggatatg catatataaa aaaatatcct agtcttgtcg
     6781 atatt
//
LOCUS       DADXTH010000108         6710 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.110, whole genome shotgun sequence.
ACCESSION   DADXTH010000108 DADXTH010000000
VERSION     DADXTH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.110"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWI97_002532"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWI97_002532"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HBC2464003.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWI97_002533"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWI97_002533"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HBC2464004.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWI97_002534"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWI97_002534"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HBC2464005.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IWI97_002535"
     CDS             complement(4028..5044)
                     /locus_tag="IWI97_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2464006.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="IWI97_002536"
     CDS             complement(5146..5343)
                     /locus_tag="IWI97_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464007.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="IWI97_002537"
     CDS             complement(5315..6634)
                     /locus_tag="IWI97_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBC2464008.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1769 a         1635 c         1158 g         2148 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact
//
LOCUS       DADXTH010000109         6350 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.111, whole genome shotgun sequence.
ACCESSION   DADXTH010000109 DADXTH010000000
VERSION     DADXTH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.111"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1464
                     /locus_tag="IWI97_002538"
     CDS             <1..1464
                     /locus_tag="IWI97_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBC2464009.1"
                     /translation="RRDSVVVALTPIPKQTQLTIDQQTITTLEDIPQGHKIALGDLKA
                     GDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVHPSHYLFENRTFQ
                     GYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFDHITILKHPYGCS
                     QLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGEYDEERVKFLVAQ
                     DVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLSGITANPLLGAFS
                     DYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFKQYFLSYGEPVYE
                     NPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLSLLQAPGNDLVAA
                     SALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDFNAGVLLEQPMEV
                     ILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            1503..2897
                     /gene="uxaC"
                     /locus_tag="IWI97_002539"
     CDS             1503..2897
                     /gene="uxaC"
                     /locus_tag="IWI97_002539"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBC2464010.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            3056..3739
                     /locus_tag="IWI97_002540"
     CDS             3056..3739
                     /locus_tag="IWI97_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBC2464011.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            3741..4577
                     /locus_tag="IWI97_002541"
     CDS             3741..4577
                     /locus_tag="IWI97_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBC2464012.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            4588..6342
                     /locus_tag="IWI97_002542"
     CDS             4588..6342
                     /locus_tag="IWI97_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2464013.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         2026 a         1227 c         1273 g         1824 t
ORIGIN      
        1 cgacgagaca gtgtggtcgt cgcattaacc cctattccaa aacaaacaca actgacgatt
       61 gatcagcaaa cgatcaccac tcttgaagat atcccgcaag gtcataagat tgcattgggt
      121 gatttgaaag ctggagacaa cgtgatcaaa tatggctatc caatcggtca tgtgacgaca
      181 gacgtaactg ccggtcaatg gctgcacact cataatgtaa aaaccaatct ttctggtgaa
      241 ttggattatc gatatgaaaa agatgtccat ccaagtcatt acctttttga gaaccgaaca
      301 tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc gtaatgattt gtttttagta
      361 cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg tcaaacagtt caaggaaaaa
      421 catccagatc tgggacagtt tgaccatatt acgattttaa agcatcctta tggctgttcg
      481 cagcttggaa aagatcatca aaatactcga gaaattttag cggacgcagt aaatcatccc
      541 aatgccggag gagttttagt atttggatta ggttgtgaga acaatacagt tcctgaattc
      601 aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat ttctagtggc tcaagatgtt
      661 tacgatgaga tcgaacaagg tgtggctcta ttagaagagc ttttaacagc ggcggaaaat
      721 gaccagcgca tatcagtccc gctctctaag ttgaatgtcg gtttgaaatg cggcggttct
      781 gatggtctat cgggaataac tgctaatcct ttacttggtg cattttctga ctacttgatt
      841 gctcaaggag gcagcacgat cttaacagaa gtgccagaaa tgtttggcgc agaacaagta
      901 ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa tcgttgactt gatcaatgac
      961 ttcaaacagt atttcttgtc ttacggggaa ccagtttacg aaaacccatc tcctggtaat
     1021 aaagcaggtg ggatcactac attagaagat aagtctttag gctgcacgca aaaatcagga
     1081 agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa tcagaaaacc agggcttagt
     1141 ttattgcaag ctcccgggaa tgatcttgta gctgcctctg ctcttgcttc atccgattgc
     1201 cagttggtct tgtttaccac aggaagaggg actccgtttg gctcatatgt cccaacggtc
     1261 aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc attggatgga ttttaatgca
     1321 ggcgtcttat tagaacaacc aatggaagta atcttagaat cgtttgttca aaaaatcatc
     1381 gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg acgttcgaga aattgccatt
     1441 tttaaaaacg gagtaacact ttgattttca taaaacagta taaaaagaaa gaaggaagat
     1501 ttatgttttt atctgatgat tttttattaa aagacgattg ggcgaaaaag ctttaccatt
     1561 cctacgcaaa aaacatgccc attattgatt atcactgtca cttagatcca aaagaaattt
     1621 acgaaaacaa caatttcagc aatctgacag aagcttggtt aggaggcgat cattacaaat
     1681 ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat cacaggaaat gcttctgatt
     1741 ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat tatcggaaat ccgctttaca
     1801 gttggtcaca tcttgaattg aagcgtttct tcggtattga cttgttgata aatgaagaaa
     1861 atgcagagaa aatttggaca caagcaaatg aaattttggc agctcctgat tttcgccgtc
     1921 gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac aacagatgat cccgcagacg
     1981 acttgcacta tcatcagttg cttgctcatg aagaaccaga tttgaaggtt ctaccttcat
     2041 tccgtccaga taaagcactc aatattgaaa aagaaggatt taatgcatgg gtcacactac
     2101 tggaagaagc agctgaccaa acgatcacca cctatcaaga actgatagaa gtcttagggc
     2161 agcggataga atacttccat caacatggat gtcgtgtttc cgaccacgct ttagacatct
     2221 tacgctataa agaagcggat gaaacagtat tagaagagat ttttcaaaaa gcacgagcta
     2281 acaaactatt atcttcagat gaaatcgatg cttatcgtac agaaacactg atacatttga
     2341 cccacttcta tcattctcat aattggacga tgcagctgca catccatgct tatcgtaact
     2401 gtaatacaca aatgttggaa aaattagggc ccgataccgg ctatgatgga atgaatgatc
     2461 tttctttgac tattcctctg caaaaattat tgaaccgtgc agaagaaacg gatcagttgc
     2521 caaagacgat cctttattca ttgaatccga atgactatcc tgcgatttta gctttgatgg
     2581 gctgtttcca aaaagaacag aatggaaaac tgcagttagg ttctggatgg tggtacaacg
     2641 atactcgagc tggtatgcgc gatcaattga cacaatttgc tgatggtagt gctttgggga
     2701 attttgtcgg catgctgaca gattcaagaa gtttcctgtc ctacactaga catgaatatt
     2761 tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat gcgaggagaa gcacctgaag
     2821 atgaacaact tttaggaagt ctagttcaag cgattagtta tacaaatgct aaaaattact
     2881 ttggcttttt tgcctaattg attaatagta acagtaacga atcaaaaaca ggatagtgaa
     2941 ataacggaat ccgtttatcc acgttttaga gaagaagacg gaccaaattt gctccgtctt
     3001 cttctcttgc tgttctgctt cattgcctat aaacctttat gaaagaagag ataacatgcc
     3061 tactatccat attgccggtg attctactgc ggcagaaaaa gatgaaaaca agcgtcccga
     3121 gacaggttgg ggagaaaaaa tctctgctta tttttcaccc gagatccaga tcaataacca
     3181 agcaaagaat ggacgcagca ccaaatcatt tatccaggaa ggaaggttag cccgtctagc
     3241 ttcacagttt cagccgaatg atttcttatt tgtacaattt ggacataacg accagaaaat
     3301 ccaagaagaa agaggtacgc ttcccttcgg tagctattta aaaaatctgc agatattttt
     3361 tgaatgcgca aaaaaagcga acgtccaaat ggtcttactg acatctgtga cgcgacgaga
     3421 ttacttagaa aacggaacat tgaatccaga tatacttgga gactatccaa aagcgatgcg
     3481 tgcatttgct gagaaacacc atattcctct tttagatgtg ttctcaagat cacaagaatt
     3541 gttccagacc ttctcaaaag aagagacaaa aaaattctac ctgcatctta tacctgatac
     3601 ttgtaaaaat tacccagaag gactaaaaga caatacccac tttagtccag aaggcgctga
     3661 taaagttgcc cgaataattg ttgaattgat aaaagaatcc cgtctgcctc ttgctaatta
     3721 tttacaaaaa ggagtttgag atgtttccat taaatttagg catccgtgcc cacgatttag
     3781 atgttcgaaa tcgtgaagaa atcgttcaaa aagtatcaca gcttcacttg tcccatatcc
     3841 aatttgcacc acaaaaagca tttcctgaac aacttaaatt aactgacatg acacttggga
     3901 gtgctgccta ttttggtgaa tacttccaaa aaaacaatat cgaactttcc attttaggat
     3961 gctatatcaa cctatccagc aaaaatcaag aagttcgtgc acaagcattg gcaactttca
     4021 aacagcaaat tgctctgtgc tctgcctatc aagccaagat gattgctacg gaaaccggta
     4081 gtgtctctgt cggttacaca aaagaaaatt ttactgaaga agcttatcag atcgccagaa
     4141 attctatcat tgaattagtc gctcatgcag aaaattttgg cgtaactgtt gctgttgaag
     4201 cagggatcaa tcatccaatc tataactatc agttagctaa acgtttaatc gatgaagtag
     4261 cttcaccaaa tttaaaaatc attttagatt gtgcgaattt gatgcataaa gaaaatcatg
     4321 ctgatcagga aaaagtgatc cgaggtgcct tggacaattt acatggttat attacagcgc
     4381 ttcatctcaa agactatatc atggaaaatg gaaagatccg catggtacct gttggcaagg
     4441 gctgtttaga tttccgcccg gtactgcatt atatcaaata tgagcgtcct ctaatgtacg
     4501 ccactcttga agctactcct gagatttatg ttcctgaagc aatagaacac ttgcagttta
     4561 tttaccaaga ttgttaagga gaagattatg gaggaacgaa tacagaaaag ttatcaaaaa
     4621 tatcatgcaa atgcttttca gacattgctg catctttatc agggaaatta ttggcgtttt
     4681 gccatgtcag gactcttttt tattatcaaa catcttccat cttggttaat gccgatcgct
     4741 attgccaatg taatcaatgc agcttcatcg aaagattctt ctcagttgca ggccatttat
     4801 ttcaacgctt ttttgatggc ttgtctgatt ggtcaaaata tattgacgaa ttattttcat
     4861 gtaaaatacc atagcacttc gatccgtcaa gtagaagctg gactaagagc gacccttatc
     4921 aagaaaatcc aagaactatc tattccttat caaaaagaat tgcaatctgg acgaatccag
     4981 tcaaagatca tccgtgatgt agaagctatc caaacgttgt cctctcaagt atttgtcagc
     5041 ggattaaata tcgctgtcaa tttattcgta gctttaggta tcacgcttta taaaagtcct
     5101 gttgttttca tgtttttctt attgactgtt cctctcgccg cattcgttac tctctttttc
     5161 cgtaaaagga tcagacagtc caactcagac ttcaggcaag aaatggaaga gacttctgca
     5221 aaagtgatcg aagtgatcga attagcaccg attgctcgag ctcatgggtt agaaaaacac
     5281 gaaacaactc gcttaaataa gcattttact caaatctatg aaaaaggatt ccacttagat
     5341 atcctgcaat ctgttttcgg atcagtcagc tgggctagtt ttcagatatt tcagctgatt
     5401 tgtttgattt tcactgggat attagcttta caagggcgca tccaacccgg cgatgtcgtg
     5461 atgtatcaga cgtatttcac gacgatcgtc aattccgttt ctggaatgat cacgctgatt
     5521 cctacgattt caaaaggcat ggaatctatc acttctattg gcgaactgtt gattgctggc
     5581 gatgtagaaa cttatcacgg taagaaaaaa atccaacatt tggatggaaa ttttcgtttt
     5641 gaggatgttt cttttcttta tccggactca ctcaaaccga ttatcgatca tttttctcta
     5701 gaggtgaaag ctggagaaac aattgctttt gttgggccat ctggctctgg aaaatctaca
     5761 ctccttaact tattgattgg atttatccag ccgacttcag gaaaaatttt tctagatgat
     5821 cagcctttgg atgaactgga tatgcgaagt gtgcgaaatt atttagccgt tgttcctcaa
     5881 accactttgt tgttctccgc ttcgatcaaa gagaacatca cttatggctt gaaaaacgta
     5941 tcaaaagaac gtttagatga agtcatcgaa gctgcgcaac tttcttcact gattgaccag
     6001 cttcctgatg gtctggatac cttagtaggc gaacatggca acaagctgtc tggtggccaa
     6061 aagcaacgta tctccattgc acgagcgttg atacgtcagc caaaaatcat tttgctagat
     6121 gaagcaacct cagctttaga taaccagtca gagaaaaaaa tccagcaagc cctgaattac
     6181 ctgacagaaa cacccactac ttttattgtg gctcatcggc tttcaacgat caaagaggca
     6241 gataaaatcg ttgtgatcga tgaaggaaaa atcgttgaaa taggtactta cgaaaaactg
     6301 ttagctcaaa aaggctattt ctaccgcttg aaaaatgaat gacaaaaaat
//
LOCUS       DADXTH010000110         6317 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.112, whole genome shotgun sequence.
ACCESSION   DADXTH010000110 DADXTH010000000
VERSION     DADXTH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.112"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..121
                     /locus_tag="IWI97_002543"
     CDS             <1..121
                     /locus_tag="IWI97_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2464014.1"
                     /translation="QGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            137..220
                     /locus_tag="IWI97_002544"
                     /pseudo
     CDS             137..220
                     /locus_tag="IWI97_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            362..1501
                     /locus_tag="IWI97_002545"
     CDS             362..1501
                     /locus_tag="IWI97_002545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2464015.1"
                     /translation="MIKNEYRNLLKHIYWGSFDSGVIFIFLSLMIWDDTENMVTVALA
                     FSIPIVIDTVIDYFFSSFSDHHNRKKLFIIGNIGSAIALSFYGFAQSIYILYGLIFIK
                     SLFAKLYQSSLDPYIRENIKEDLYMDFLSQRNIQMSVGASIGGFALMMLYGFLQNIAL
                     IFVVSGLIELYSTVYLFKLKDNKLQRRKEKEDAIDLEWIKYITFIYTVEGFGIALIVN
                     RMLIYIHEFQGIPLGNVGFVFFVVYGVSSVMAARIYKRFNKISLKAMLITSFLLQAFL
                     LIIFIQINQLFFIIIVWFIFELVANIADIYASDKINSSLFTEIGKRLSKFRIMIAIGN
                     VLGQFIVSRIWDQFGMSMSFYFSSIILILLSFVILLKNEKHFEKN"
     gene            1953..2345
                     /locus_tag="IWI97_002546"
     CDS             1953..2345
                     /locus_tag="IWI97_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2464016.1"
                     /translation="MNQIQIKGAILEVLNLPSMNGIEDENLRRLINSLVIELYKYQAE
                     SERKKIKERQAQGIEIAKKKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTG
                     INRRTFRRYRARYNVTVDQRKNKEKRDS"
     gene            2345..4489
                     /locus_tag="IWI97_002547"
     CDS             2345..4489
                     /locus_tag="IWI97_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBC2464017.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPLFTDETFIT
                     FGFGHLVELAEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVAELLKKANT
                     IIVATDSDREGENIAWSIIHKANAFSKDKTFKRLWINSLEKDVIRSGFQNLQPGMNYY
                     PFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIFQRQEAIE
                     NFKKEPFFEVEASIKVNQGSFKGVLSPTQRFKTQEELLAFVSSKQAKIGNQEGIIADV
                     QTKEKKTNSPSLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITE
                     NEFAYLKANFGKYSGFLGLDLEMVQTEPRKRYVDGSKVQEHHAIIPTKQVPTESALAK
                     MDDLQRKIYALVVKTTVAMFLPDYLYEETKIQTKVADLLFQSIGKTPKQEGWKILFKQ
                     QTKEEKEDVQTLPLVIIGERAEVGVKSVEKETQPPKAFTEGTLLTAMKTANKTVDDEE
                     AIKILQEVEGIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKLLCQAVESQHLLTS
                     AEMTAKWETYLKKIGKREGNQENFITNIKKFIVHLLEAVPNDIEKLNFSDYQEQKEKE
                     AEKSIVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGKET
                     LVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            4623..4862
                     /locus_tag="IWI97_002548"
     CDS             4623..4862
                     /locus_tag="IWI97_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBC2464018.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            4911..4994
                     /locus_tag="IWI97_002549"
     CDS             4911..4994
                     /locus_tag="IWI97_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBC2464019.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYTDK"
     gene            5119..5856
                     /gene="erm(B)"
                     /locus_tag="IWI97_002550"
     CDS             5119..5856
                     /gene="erm(B)"
                     /locus_tag="IWI97_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBC2464020.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            6165..>6317
                     /locus_tag="IWI97_002551"
     CDS             6165..>6317
                     /locus_tag="IWI97_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2464021.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQST"
BASE COUNT         2321 a          952 c         1168 g         1876 t
ORIGIN      
        1 tcaaggattc atctcatccg ttgcgtccaa aagaaaccat atccctagaa aatcactaaa
       61 ttaccaaaca ccattagaag tttttttgag ttacgtaaat ggaaagtttt gtctcgctta
      121 atttgacaaa taataatata aaaagaaccg aagaaatgga acgctcttcg gcttttcggt
      181 gtctactgaa atcaaggtat taatgggaat aacagcctaa gatatttgga gttcagatag
      241 ggcgcgttta attttcaaac ttcgcaacag aacctatttt ctttatcaaa agtcttatga
      301 tacaattcca agtaattttt actgtaaaaa ttactttaaa agtagattag aatggtggta
      361 tatgataaaa aacgaatata gaaatttatt aaaacacatt tattggggta gttttgatag
      421 tggagttatt tttatttttc tatccctgat gatttgggac gatacagaga acatggtaac
      481 tgttgcttta gcattttcaa ttccaattgt gattgataca gtcattgatt atttcttctc
      541 gagttttagt gatcatcata atcggaagaa actttttatt attggaaaca taggatctgc
      601 tattgcacta agtttttacg gttttgcgca aagtatttat attctttatg ggcttatttt
      661 tatcaagtcc ttatttgcta agttatacca aagttcatta gatccgtaca tacgagaaaa
      721 tattaaagaa gatctttata tggactttct ttcgcaacga aatattcaaa tgagtgtagg
      781 tgcttctatt ggtggtttcg ctttaatgat gctgtatggg tttttgcaga atatagccct
      841 catttttgtt gtttcaggat taattgaact ttattcaaca gtttatttat tcaagcttaa
      901 ggacaacaaa cttcaaagaa gaaaagaaaa agaagatgca attgatttag agtggataaa
      961 atacatcact tttatttata cagttgaagg ttttggaatt gcgcttattg tcaatcgtat
     1021 gttgatttat atacacgaat tccaaggaat tcccttagga aatgtggggt tcgttttctt
     1081 tgttgtctat ggtgtttcta gtgtgatggc agcaagaata tataaacgat ttaataagat
     1141 ttcgttgaag gcaatgctca tcacttcttt tctcttacaa gcttttttat tgataatctt
     1201 tatacaaatt aatcaattat tttttataat tatagtttgg ttcatctttg aattagttgc
     1261 taatatcgcc gatatatatg caagtgataa aataaacagt agtctcttta cagagatagg
     1321 gaaaagactt tccaaattta gaattatgat tgcaataggt aatgttttag ggcaatttat
     1381 agtaagtcgt atttgggatc aattcggaat gagtatgagt ttttattttt caagtattat
     1441 tttaattcta ttatcttttg ttattttgct aaaaaatgag aagcattttg agaaaaactg
     1501 aaataaaaac ttgccccctg ctgaaaaagc gaagggggtt ttttatttta ttttgtggca
     1561 ataaatttgc cacaaacagc acttaaaaaa tgagcgaaaa cgggcgaagt ccaagcgata
     1621 tttttttact cggaaatcga agattgacga acgatcttga ccttgctttt agccgtcact
     1681 tgtgaatttc aaaaattcgc cttgtgaagg cttatttgtt gcacgataca acttgtgaaa
     1741 tccggctggt tcacaatcta tacctgtcat ttttctagat aaatggcata aattttcagc
     1801 gctaagtgac aaaagcaact aatttttcat gatagatttt taaatatctc aatctatctc
     1861 caaaattagt tgcttttgta gctatagttg actatgaata tgacacataa ttagaaaaaa
     1921 ctgtgccaat taaatataaa aatgacaaat tgatgaatca aattcaaatt aagggggcaa
     1981 tcctggaagt cttaaattta ccctcaatga atggtattga agatgaaaat ttaaggcgtt
     2041 tgattaatag ccttgtcatt gaattgtaca agtatcaagc agaatcagaa cgaaaaaaaa
     2101 ttaaggaacg tcaggcacag ggaatcgaaa ttgctaagaa aaaaggcaaa ttcaaaggtc
     2161 ggcaactgaa atttaaaaaa aatgatccac gtttaaagca tgctttcgat ttgtttttga
     2221 atggtttatc tgataaagaa gttgaagaac aaactggaat caatcgccga acgtttagaa
     2281 ggtatcgagc aagatataac gtgacagtag atcaaagaaa aaacaaagaa aagagggata
     2341 gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct acgcaagtgc
     2401 tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac tatttacaga
     2461 tgaaacgttt atcacctttg gttttgggca tttagtggaa ttagcagaac caggtcatta
     2521 tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga
     2581 ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa
     2641 ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc
     2701 gattatccat aaagcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa
     2761 tagcttagaa aaagatgtaa tccgaagcgg ttttcaaaat ttgcaacctg gaatgaatta
     2821 ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa
     2881 cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact
     2941 aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag ccatagagaa
     3001 ttttaaaaaa gaaccttttt tcgaggtgga agctagtata aaagtaaacc aagggtcgtt
     3061 taagggcgtt ctaagcccca cacagcgttt taaaacccaa gaggagcttt tagcttttgt
     3121 ttcttctaaa caagctaaaa taggcaatca agaggggata attgctgatg ttcaaaccaa
     3181 agagaagaaa acgaatagtc cgagtttgtt ttctttaagt agtttgcaat caaaagtcaa
     3241 tcagctttat aaagcgacag cgagccaaac tttaaaagct atgcaaggac tgtatgaagc
     3301 aaaattattg agttatccaa gaacagatac accatttatt acagagaacg aatttgctta
     3361 tttaaaagcg aattttggca aatatagcgg ttttttagga cttgatcttg aaatggttca
     3421 aacagagcct agaaagcgtt atgtggacgg tagtaaggta caggaacacc acgccattat
     3481 cccaacaaaa caagtaccta ccgaatctgc attagcgaaa atggacgatt tacaacgaaa
     3541 aatttatgct ttagtcgtta aaacgaccgt tgccatgttt ttacctgatt acttgtatga
     3601 agaaaccaag atacaaacga aagtagctga tttgctgttt caatcgatcg gaaagacacc
     3661 aaagcaagaa gggtggaaaa ttcttttcaa acaacaaacc aaagaagaaa aagaggacgt
     3721 tcaaacgtta ccactcgtta tcattggcga acgtgccgaa gttggtgtta agagtgttga
     3781 aaaagaaacg caaccgccaa aagcttttac agagggtaca ttattaactg ctatgaaaac
     3841 ggcgaataaa acggttgatg atgaagaagc aatcaagatt ttacaagaag ttgaggggat
     3901 tggaacagaa gcgacaagag caagtattat tgaagcgtta aaacaaaaag aatatatcca
     3961 agtgattaag aataagcttg ttgtaactga aaaaggaaaa ttattgtgcc aggcagttga
     4021 aagtcagcac cttttaacga gtgctgaaat gacggctaaa tgggaaacgt atttaaaaaa
     4081 aatcggtaaa agagaaggca atcaagagaa ctttattacg aatatcaaaa aattcattgt
     4141 tcatttactg gaagctgtac ctaacgatat agaaaaacta aatttttctg attaccagga
     4201 acagaaagaa aaagaagcag aaaaaagtat tgtaggaaaa tgtcctaagt gtggcaacaa
     4261 tattgtatta aaaaaatcgt tttatggttg ttcaaattat cctgaatgta agtttacttt
     4321 agctgaacat tttagaaaga aaaaactaac caaaacgaat gtaaaagaat tactggaggg
     4381 aaaagaaacc ctggtaaaag gaatcaaaaa caaagagaaa aagccctaca atgccgttgt
     4441 aaaaattggg gaaaagggat atattgattt tatatctttc tcaaaataaa cataaaagcc
     4501 ctttaaagag ggcttttata tattaatcac aaatcactta tcacaaatca cttatcacaa
     4561 atcacaagtg atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt
     4621 gagtgattgt gggaaattta ggcgcacaaa aagaaaaacg aaatgataca ccaatcagtg
     4681 caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg caccatatca
     4741 taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa ataagactta
     4801 gaagcaaact taagagtgtg ttgatagtgc attatcttaa aattttgtat aataggaatt
     4861 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
     4921 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
     4981 acacagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
     5041 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttgttta tagatgtaat
     5101 cacttcagga gagattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
     5161 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
     5221 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
     5281 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaat
     5341 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
     5401 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
     5461 tttgaaagcc gtgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
     5521 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
     5581 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
     5641 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
     5701 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
     5761 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     5821 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     5881 tggaaagtta cacgttacta aagggaatgg agataaatta ttaggtatac tactgacagc
     5941 ttccaaggag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     6001 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     6061 tgtattaaaa aaatcgtttt atggttgttc tgaatcggct acactagact agacagaaaa
     6121 aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac
     6181 gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcga tctctatctc gctggtaagc
     6241 ctcgcgcaga aattattcga gagtatgagc ttacgccttc ttctttcgat aaatggatga
     6301 agcaagcaca atcaacg
//
LOCUS       DADXTH010000111         6199 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.113, whole genome shotgun sequence.
ACCESSION   DADXTH010000111 DADXTH010000000
VERSION     DADXTH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.113"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..535
                     /locus_tag="IWI97_002552"
     CDS             2..535
                     /locus_tag="IWI97_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464022.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(661..1338)
                     /gene="nth"
                     /locus_tag="IWI97_002553"
     CDS             complement(661..1338)
                     /gene="nth"
                     /locus_tag="IWI97_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBC2464023.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1345..2043)
                     /locus_tag="IWI97_002554"
     CDS             complement(1345..2043)
                     /locus_tag="IWI97_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBC2464024.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2311..3495
                     /locus_tag="IWI97_002555"
     CDS             2311..3495
                     /locus_tag="IWI97_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBC2464025.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3501..3944)
                     /locus_tag="IWI97_002556"
     CDS             complement(3501..3944)
                     /locus_tag="IWI97_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2464026.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4170..4457)
                     /locus_tag="IWI97_002557"
     CDS             complement(4170..4457)
                     /locus_tag="IWI97_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464027.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4628..5980)
                     /locus_tag="IWI97_002558"
     CDS             complement(4628..5980)
                     /locus_tag="IWI97_002558"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBC2464028.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1668 a         1209 c         1023 g         2299 t
ORIGIN      
        1 tatggttttg aataattttt tgaaagaagg cttcattttg agttatctga tagtatgctt
       61 aattatcata atttgtctgc accatctttt tttatcgaaa acaatacctc gttattccaa
      121 gaataaaaaa gctgaaaaat tttgtttatt attaaataaa tttactttaa ttgctcctat
      181 ccttgcattt attatttttt ctgtactttt aagcactact cttaaaggaa aatttatgga
      241 aagaagctct catgcaatga ttctcacttt tttatggttg ttgttcacca gaatctatat
      301 ttttctgatg agcctgaaac cgcccaaatc cattagttta tgtttagtta taaatggtat
      361 ttttttatta tcacttatca tttttattac acctttagat agatacgtca cttatttata
      421 taatcctttg gaatactgga cttattttat aggtatattg gaaggcatta ttttctacat
      481 cgggtatttt cccaataaaa ataacaactt ttatttttat cgcaataaat tgtaataagt
      541 taccatccta gctactgagt atgtacattt actttttcat gcaaaaaaga taaacgaaac
      601 agcttttctc cataataaca agataattca tacatttatt tgcaattaaa gaaaccacgt
      661 ctacaggcgt ggtttctttt ttagcgcctt ttcttttaga cgcatccgat ttttcccatc
      721 ttgacatatt gatagcaaag gacatacctc gcacttggga tttcttgccg tgcaatgata
      781 tctaccaaaa aaaatcagtg tatgatgtgt ctttacccat aattcttgtg gtactttccg
      841 catcagtgtt tcttctactt ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt
      901 tgagacacgt tccacgtggg tatctactgc gattgctggt atcccgaatg catcccccaa
      961 tacgacattt gctgtctttc tgcctacacc aggaagagac atcaattctt ctcttgacgt
     1021 tggcacttgc ccatcgaaac gttcaatcag ctgctgtgca cacgctttga tgttttttgc
     1081 tttatttcga tacaaaccaa tcgtttttat tttaagaatg atttcatcga ttgaagcttc
     1141 tgctaacgca tcgggtgtcg gaaaagaggc aaacaaatct ggcgttgctt tattcactga
     1201 tacatctgtc gcctgggcac ttaagatcac tgcaatcaat aactcaaatg gattattgtg
     1261 cttgagttct ccatgtgctt cgggaaacat tccatacatc gtttctagag cttccattgt
     1321 tttttgtttg cttaacatat tccgctactc ctccaaccaa tttttcagtg agactttcgg
     1381 caattcttct tgttgagctg actgttctac ttctctttga agaagctgct gttttcttcg
     1441 tttttggtct tcttccactt gttgttttgt acgtagattt tttctttccc acgatagcaa
     1501 aatgcggtca acgtaattaa aactataagc ctgatttaat acggcttccc gcagtgctag
     1561 cttcaaaatt tccggctgat aatggtcttc ttctagccat tgtccgatcc gctggaactc
     1621 gatcgaagaa agcgggcgtc caaattcttg ttcaaataat tgatacactg actggatttc
     1681 tttttcttgt gttttttctt cgtatttttt ttcttgtgtt tttaaatact cacctaatag
     1741 atcatagatg gggtaaagat tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc
     1801 tatcttgata aatccatttg ttacgagacg atccagtatt tgaaaaatct gatcttgttt
     1861 gattcccata tccatagcaa tcatctgcaa atcagggaaa gtatctcctt caagctgtgc
     1921 catatgcaac tgtagaacaa ataaaaattc ttcagattgt agaccgatcc tatgataatt
     1981 tttaagtaaa agattagata tgactgtttg tccagcgctt aagtaatttt ctaaattcaa
     2041 catgatttct cctccgctca atcacagtat acgtaaaaaa aacatgggaa gcaagaacag
     2101 gtttcttttc tatttacttc actgatcggt cagatttaca atgtcgtcta cttgattttt
     2161 gtttgttcat gtttttgatg cgtatcttca cttttcggaa taaataaatt aagtagattt
     2221 cgctgttcct tagaatgttg acaaggaaac aaaattacta tacactattt ttgtttccaa
     2281 gttaacatta ttgcataaag gagtttttac atgtacagtt tcacaaataa acattctaaa
     2341 gtttttggat acgtctctgt cttattcact ggattaggat ttactatcgt cagtcctgta
     2401 cttccgtttt taacgctgcc ttattctcac tccatacatc agcaagcttt ctatatcact
     2461 ttgctcatgt ctgtttatgc tctagctgcc ttcttatctg cccccattct cggtagctta
     2521 agtgatcatt ttgggcgtca tccgatcctt attatcagtt tattaggatc aagtatcgga
     2581 tatttgcttt ttggtttagg aaattcgata tggatgttgt tccttggtcg tatcattgaa
     2641 ggattgacgg caggagaaat ctccacctta tatgcttact ttgccgacat cacggaacct
     2701 aatgaaagaa ccaaagtttt tggatggatg ggtgctttag tcggtatagg aacaacgctg
     2761 ggtcctatta tagggggtct attagctgag ttggggaatt ctgttcctat atttattgga
     2821 gcactcttta ctttcttaaa tgccgtttat ggctacacat ttatgctaga aagccttcca
     2881 ataaaaaaac gttcagtaga tttaagtttc tcccatgtaa gaccttttca ccaattacaa
     2941 caattattca agatttcatc cgttattcct ttattgacag caggttttgc tgtttggttg
     3001 gcagctggct ctcttcaatc tatcttttct caattttcga ttgacgcctt tcagtggaag
     3061 gctgggttgg ttggtttaag cttctctttg attggaatct tggatactgt ctcgcaattg
     3121 ttcattatgc ctcgattgct caaaaaattt tcagaacagc agatcacaag aatagggatg
     3181 ttcagtgaaa tcctcgccta tctattcatc acattatctg gaattcttct actgccgatt
     3241 ttgtttttat ttgggattat ctgttatggt ttcggtgatt ctatttttac tcctgtattc
     3301 aatggccaac tatccaattc tgtctcagat aaccagcaag gtcttgtaat gggcggtacc
     3361 caaagtatac aatctctatc gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct
     3421 gcagcaccat ttcttccgac ggtattcgga tttttgctta tgatttttgc ctattatcac
     3481 tacaataaaa aatagtagct tcatttgcta cttcgtataa attctctgta tcgaccgata
     3541 aaacgaaata ctgtgtcaaa ttcttctttt gacatatttt caaatacagt agcatctcgc
     3601 tgtacccatt tttgatggag ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc
     3661 aattgaaaat agatttcttt tttattctct tcgctttgat aagtgtttat gatccctcta
     3721 gctatcagtt tttttgttag cttactgatg gctcctctag tcatgtaact agcattcgct
     3781 aattttgtca cgttaggatc agggattttc ccgatatgtt caatcacttc tagttcattt
     3841 agagtaaatc ctgcaaattc tttttccatt tctggtttat tacgatgaat aatctcatta
     3901 gctaaatcca gatattcttc tattagcagt tgtcttttat ccatttctac accttctttt
     3961 tctttcatta tattgttttt ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa
     4021 aacccaatct gaaaccaaga aaaaaagggg ttgacaaagc ttgtcataac ccctttttct
     4081 gattaaaaga tagtaaagag acagtcatcg gtaattgtga tgattttttg tcatcactta
     4141 aggatgagcg gccttttcaa aaactgtctt taaagatcat catcagtttc tagcacatat
     4201 ggctgacctt catatgtgta ctctgtattc aatgtgccgt cattttcatc tgagtagatg
     4261 cgtctactga aaggaaaacg atgagaaaca ttatctttgg cgtcatttgc tggcatcgtg
     4321 taatattcct cttgcgtttc attcaactga gagagtactt gatctgccaa ctcatcaata
     4381 ttgatcgtta atttcttcga agcactttta taatcttcta aaaatgcatc ttgttggaca
     4441 aaatattctt tattcatctt ctttctcacc tttcttttca aaatcaaata aagctgcttg
     4501 ttcttttatg taacagcttt atctttgctg cttattgtga caaaaaattc gaagaaagac
     4561 aactaattgt acttttcaca aaaaaagaca gagggtcgtg acaaaattgt gtcacaaccc
     4621 tttttcttta ttgtgcagtt tcgatttcgc ctagcgctgc ttgtgcaagt aggttcaagt
     4681 aattccatgg gcggtcaaaa tgtggttgga agaagaaatc tgaaagtgcg agatcttcta
     4741 cagtcatttt attttgaaca gctaaagaca tggtatttgc tgattgtgta atgtcgtatt
     4801 tggacatgaa ctgagcacct acgatacgat tcgttccttt ttcatagact agctccatca
     4861 ttactttttc agtagttggc atgaactctg gacgataatt atcttcaaat acagtcgctt
     4921 ggacttctag atcgttcaaa gttgcgcttt ctttggtcac acctgtcgaa ccgatcgtcc
     4981 agccaaatag gtaaagtcct gaagttcctt gagtaccacg atacgccatt ttatgatctg
     5041 tcaagttatt tcccacaagc aaaccttgac gaacggcatt tgttgccaaa ggaatatagt
     5101 tttgcgtgcc acttggattg tagttgacta ctgcagagtc tccagctgca aaaatgtctg
     5161 gattacttgt ctgcatgtat tcattcactt tgattgcccc gtttggcagt gtatctactt
     5221 ttccttcgat cagttttgta tttggacgga agcccacaca taaaatgacc atatctgtat
     5281 caaatgtatc tgttggtgta gctacttgtt tcacttttcc ttcttcatct gtgataaatt
     5341 cagtaacatt ttcgcctagc gcaagagtaa caccgcggtc tgtcaattct ttttctaaga
     5401 tatctgtgaa tggtttatct agatatttat tcaagatgcg atctaatcca tcaatcaatg
     5461 tcacttgttt tccagattcg acaaaagctt ctactaattc aataccgata tatccaccac
     5521 caacaattac tacacgtttt gcttctttcg cttgtgcaat aatttcattt gcttgattgt
     5581 agtttttgca taggagtaca tttttcgcat cgattccact aatcggtggg ataataggcc
     5641 atgaaccagt tgtcatcact aatttatcat aagaaaccgt ttctgtttct cctgttttca
     5701 gatcttttgc cgttactgtt ttattttctg tatcgacgtc tgttacatcg tgttccattt
     5761 taactgttgc tcctaatgaa gataattctt caggatttga gtagaacaag cctgctgggt
     5821 cctttactac tccgccgaca taaagtgcga taccgcaaga taagaaagaa atattgtcat
     5881 ttctttcata tactgtcact tctgcttctg gatgattttt taaaatactt tttacagctg
     5941 cagtacctgc atgagtacag ccaattacta ctactttcat ttatggtccc tccgattaac
     6001 attttgtcct tcgacaaaac tactataaca gaaataaaac ggatacaaca gtttaatgct
     6061 tgtgaattca caaaggtttg tttgtagttt cattcctttt caagcataag aacccaatat
     6121 ctacaagatt cttattttaa taatattcac ttttatatct aagattgtga ataaaagaca
     6181 aaaaaataaa aaagctagc
//
LOCUS       DADXTH010000112         6107 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.114, whole genome shotgun sequence.
ACCESSION   DADXTH010000112 DADXTH010000000
VERSION     DADXTH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.114"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            229..1203
                     /locus_tag="IWI97_002559"
     CDS             229..1203
                     /locus_tag="IWI97_002559"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBC2464029.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            1373..2773
                     /locus_tag="IWI97_002560"
     CDS             1373..2773
                     /locus_tag="IWI97_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBC2464030.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(2851..3573)
                     /locus_tag="IWI97_002561"
     CDS             complement(2851..3573)
                     /locus_tag="IWI97_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2464031.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3652..4158)
                     /locus_tag="IWI97_002562"
     CDS             complement(3652..4158)
                     /locus_tag="IWI97_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBC2464032.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4281..6080
                     /locus_tag="IWI97_002563"
     CDS             4281..6080
                     /locus_tag="IWI97_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBC2464033.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         1998 a         1231 c         1143 g         1735 t
ORIGIN      
        1 ttcggctaaa agagaatcgt taataaatga taaataaatt aaaaatttct tcaaagttta
       61 atgaaataat tgatatataa aaatagtgaa ataacgcaca tgtaagcttt tacatataat
      121 acaaccttgt tatctgttat attttatagc caaatagcgt gacatggggc ttgagaaatg
      181 ttagcataag catgggaaaa aacaaatcaa tggaaggatt gatactaaat gactgcagct
      241 gcagaaaaaa aagatcatca aaaagttatt cttgttggag acggcgccgt aggttctagc
      301 tatgcctttg ctctagttac tcaaaatatc gctcaagaag taggtattgt ggatatcaat
      361 actgctaaaa ctgaaggaga cgcaattgac ttgtctcacg ctttggcatt tacttcacct
      421 aaaaaaatct attctgctac ttacgcagac gcacatgatg ctgacttagt tgttattaca
      481 gctggcgcac cacaaaaacc aggtgaaact cgtttagact tagtaaacaa aaacctaaaa
      541 atcaaccgcg atgttgtcac acaaatcgtt gaatcaggat ttaacggtat ctttttagtt
      601 gccgctaacc cagtagatat tttgacttac tcaacttgga aattctcagg tttccctaaa
      661 gaacgcgtaa tcggttcagg tacctctctt gactcagctc gtttccgtca agcacttgcg
      721 gaattagttg acgttgatgc acgtaacgtc catgcttaca tcttaggtga acacggagat
      781 tcagaattcc cagtttggtc acatgcaaat gttgccggct tgcaaatcta tgaatgggta
      841 aaaaacaacc cagaaatcga tgaagaagct atggttaacc tattctttgg cgtacgtgac
      901 gctgcttaca caattatcga gaaaaaaggc gctactttct atggtatcgc agtagcatta
      961 gcacgtatca ctcgtgccat cttagatgat gaaaatgctg ttcttccttt atcagtattc
     1021 atgaatggcg aatatggatt gaacgacatc tacattggtg cacctgctgt tatcaaccgt
     1081 caaggtatcc aaaaagtaat cgaaattcct ttaagtgaca gcgagcaaga ccgtatggct
     1141 gcttctgcta agcaattaaa agaaattcta gatgaagctt ttgctaaact agacgctgaa
     1201 taatctttga acatgacgga aaacatctat tcggtgtttt ccgttttttt attttatcac
     1261 ccttatggtt tctaaatttt ttcataaagt agagataacc ttcgtctttt cctctaaatt
     1321 atgctatcat gagaccgttc caccctaaac ttttacttaa ggagttgtcg atatgacaag
     1381 atcttcgcat cgaaaaaaat cgaaacatgt tgctttgctt tcagttcttg gtgttctctt
     1441 agtgctgatc agcttttata cctatcgcag tacatactac acaaagcatt ttttgccgaa
     1501 tacagaaata aatggaataa atgtcagcaa tctaacagtc gaaaaagcaa atgaaaaatt
     1561 aaaagaagct tattctgata aaaaactggc tattaaagaa gatggaaaag tctggcagga
     1621 agtaccaaaa tccgagctcg gctacaaaga tgatttcact tcggagcttt ctccgatcct
     1681 aaatgaacaa aacggatgga cttggggaat gacttatgtc tcagcggcag aaaaacaaga
     1741 aatagatcct gtcagtcaaa accaccaaaa actagacact accgttcaga cattgacaac
     1801 taagctgact gatttgaaca aagaccggac acctactact gatgcaacga tcgaaaaagc
     1861 aggtgactct ttcgttatca agcctgaagt caatggaaat acgatcgacg tcgatgctgc
     1921 ggtcaaacaa ttaaaaagtg cggtaaactc tggtaaagat acaattgaat tgactgaatt
     1981 caaagaaaaa ccaaaagtaa catctgaaga cagctcactg aaagaacaac tcgcatccat
     2041 gaatgctatc gcaaatgtaa aagctactta cagtataaac ggtgagactt tccagatccc
     2101 atcaagtgat attatgagtt ggctgaccta caatgatgga aaagtcgatt tagatacaga
     2161 acaagtacgt caatatgtaa cagatttagg cacaaagtat aacacaagta cgaatgacac
     2221 aaaatttaaa agtacaaaaa gaggagaagt tacggtccca gttggcactt acagttggac
     2281 gatccaaaca gacagtgaga cagaagcatt gaaaaaagcg attctggcag gccaagattt
     2341 tactcgttca cccattgtgc aaggtggcac aaccgctgat catccattga tcgaagatac
     2401 gtatatcgaa gttgatctgg agaaccagca tatgtggtat tacaaagacg gaaaagtggc
     2461 ccttgaaaca gatatcgttt ccggaaagcc aactacacct acacctgctg gagtcttcta
     2521 tgtttggaat aaagaagaag atgcaacttt aaaaggaaca aatgacgatg gaacacctta
     2581 cgagagtccg gtaaactact ggatgccgat tgactggaca ggtgttggta tccacgattc
     2641 agactggcaa cctgaatacg gtggagactt atggaaaacc cgcggttcac acggctgcat
     2701 caacacccca ccaagtgtaa tgaaggaatt attcgggatg gtcgaaaaag gaacgcctgt
     2761 attggtcttc taacaaaaat aatatataaa aagaaagcac ccgtacgatt ggtacaggtg
     2821 ctttcttttt attatctaac aatgtgctac ttacatttca gcagcagtaa agacttctga
     2881 aacatcatca tcgtcttcta gcttatctac tagacgttcc aattgctctt tttgttcctc
     2941 atttaaatca atcgtcgttt gtggaatcat cgtcaattct gcttgcgcca aagtgaactc
     3001 tttttccaac tcatctcgta cagcagcaaa atcttctggt gcagtataaa tctcgaatac
     3061 ttcaggagaa gtttgcagat cttctgcccc cgcctctaat acatcctcaa acatcgtctc
     3121 ttcatcaaca gtcaacccat cgcgttcaat agcgatatat cctttgcggt caaacaagta
     3181 actgactgaa cccgtttctc ccagtgatcc accgtttcga gtaaaagcta ctcggacatt
     3241 tgtcgctgtc cgattacggt tatctgtcaa tgcatggact aagattccta ctccacccgg
     3301 accatatcct tcatacgtga tttcatcgta attcgtttca tctacggcac tcgtagcctt
     3361 tttaatcgcc cgttccacat tgtcgttagg catatttgca gcttttgctt tgtccacaac
     3421 aagccgcaaa gctggattca tagatggatc aggaccgcct gctttagctg ccatataaat
     3481 ttcacgagaa agtttctgaa agactttccc tcgttttgca tcttgggcat tttttcttcc
     3541 ttggatatta tgccatttac tatgtcctga catgattttt cctcccttgt tcttttgaga
     3601 atacacttct attctagtac tatttcacaa aaaaacaaag ccacagccaa tctataaacg
     3661 cttcttgaat gccgcaaaaa gcacacataa tatcacagca gtcagagcac cgatcgaatc
     3721 caacatgaca tcttggaaca taggtgttcg tccaccggtc atcatctgat ggaattcgtc
     3781 caaacctgcg taccctgttg ctgaaagcca agcaaggatc gctgtcagcc accaaatttt
     3841 tagcttgggg aaaacaccta aaaacaagct gcctcccaaa ataaaatagg taccgaaatg
     3901 tgcgcccttc cttaaaaaga actctaaaaa tttggcgtag cccagatggt cgatactcac
     3961 catacttcca ccataattaa aacgtatacc cgctaatgtg tctttaaatg gctgattagg
     4021 cagccagctt tctaatcgcg aaacttgtga ttgctgtccg taagtttgag aagaactgta
     4081 aaataagatt ataacgacta tcagtgcgat tactaaataa atatttccgt ttttcatttg
     4141 tttttttgaa gaattcataa gtcacctctg tattttatct tagacttttt ttgaagaaac
     4201 aacaatcatt ttcatagaga cttcttccat aaaaaagtgc tatacttaat tttgtattac
     4261 aatatgaagg gagttttttc atgagttatt cattaaattg gaatctagat tctatttttt
     4321 ccggaggcag tcattctgat gctttgaacc aacggatgaa gcaattggaa gaccaaatga
     4381 acgagtatta tcaccgcgta acaaaatggt cacctagttc tgacaatgca gagcagttaa
     4441 acgctattct tcaattgcaa gaaacgatta cgaatggttt tacccaatgc agcagctata
     4501 ttactgcatt gctttctgcc aatgtcaatg attcagatgc aaaggtctta tcaggcaagc
     4561 tttacgcaat gctgcctcgt ctgcaatcag ccgaaactgt attgtcaaag aaatttgcag
     4621 aaatatctga caatgattgg aatcagatgc tttctcaatc ttcgtttgaa acaattgctt
     4681 ttcgtttaaa cgagattcgt cgtgatggct cacaattatt atcagaagct gaagaaaaca
     4741 ttataaatac gctttcttta gatggtctaa atgcttggag cagtcattat gatacgattg
     4801 tagccagcat ttcgattccc ttcgaacaag atggacaagt agtcgaatta tctgctggac
     4861 aagccttcaa taagatgatg ggcgatccag atccaaaagt cagagaaaca cttttcgcag
     4921 catgggaaga agcctggaaa gaaaaggcac ccctcttcac ggatactttg aatcatttag
     4981 atggttttag actttctgac tatgaacttc atggtgtcac agattttcta caaaaaccat
     5041 tagagtacaa tcggttgaag aaagaaacat tgtctgtcat gtgggataca atccagaaaa
     5101 acaaacaacc ccttgttaat tacttgacta gaaaagctaa tcttttcggt aaagaaaaaa
     5161 tggaatggca agatcaagat gcacctatta ttttaggcga tttgaaagaa aaaaccttta
     5221 gttttgacga agcagcagca tttattatcg aaaactttaa caaattcagt ccaaaaatgg
     5281 ctaaatttgc ccaatctgct tttgaaaaga gctggatcga agcagaagat cgtcctggga
     5341 aacgtcctgg tggttattgt actgagttac cagaaacaaa agaatcaagg atctttatga
     5401 cctacagtaa ttctgttaat gaggttgcga cacttgctca tgaactagga catgctttcc
     5461 atagcagcac gatgtgggat ttaccttctt taaatcgcga atatgccatg aatgtcgcag
     5521 aaacagcaag tacattcgca gaactgatcg tagccgatgc tacattaaaa gtcgcaaaaa
     5581 caaaagaaga aaaaatcaat ttgctagata caaaactgca aaatgcttta gctatgttca
     5641 tgaatatcca ttcccgattc atttttgaaa atcgattcta tgaagcacgg caagaaggat
     5701 tagtctcaga agataagatc acagagatga tggttgaggc gcaaaaagaa ggctatcaag
     5761 gagctttagc tacttaccat ccatatttct gggctgcaaa attgcatttc tttattgatg
     5821 acgtaccgtt ttataacttc ccatacacat ttggctacct ctttagtttg ggcatctatg
     5881 cttacgccaa ccaaaaggga gcaaattttg aagaagaata tatccattta ctacgcgata
     5941 ctgcctcaat gacgacggaa gatcttgcga agaaacattt gaatgtcgat cttacccaac
     6001 cagatttttg gcaagcaggt atcgacatgg tgactgaaga tatccaaacg ttcatggaat
     6061 tgacagaaga atttatctga tttctatttt atcacaataa aaaaaga
//
LOCUS       DADXTH010000113         6066 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.115, whole genome shotgun sequence.
ACCESSION   DADXTH010000113 DADXTH010000000
VERSION     DADXTH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.115"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(174..476)
                     /locus_tag="IWI97_002564"
     CDS             complement(174..476)
                     /locus_tag="IWI97_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464034.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(696..1062)
                     /gene="ssrA"
                     /locus_tag="IWI97_002565"
     tmRNA           complement(696..1062)
                     /gene="ssrA"
                     /locus_tag="IWI97_002565"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1233..2006)
                     /locus_tag="IWI97_002566"
     CDS             complement(1233..2006)
                     /locus_tag="IWI97_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2464035.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGTGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2150..2500
                     /locus_tag="IWI97_002567"
     CDS             2150..2500
                     /locus_tag="IWI97_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBC2464036.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2481..3197
                     /locus_tag="IWI97_002568"
     CDS             2481..3197
                     /locus_tag="IWI97_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2464037.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3197..4645
                     /locus_tag="IWI97_002569"
     CDS             3197..4645
                     /locus_tag="IWI97_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2464038.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4715..5224)
                     /locus_tag="IWI97_002570"
     CDS             complement(4715..5224)
                     /locus_tag="IWI97_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464039.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5214..5939)
                     /locus_tag="IWI97_002571"
     CDS             complement(5214..5939)
                     /locus_tag="IWI97_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464040.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2069 a         1050 c         1169 g         1778 t
ORIGIN      
        1 acttataatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
       61 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatcccttt
      121 tataagtatg gtgataaaat ccgttccggg ctaccttttt taggtagcct actttaatct
      181 ttataccttt ctggatcaac gaaagtatac ttcaaatagt cataacgccg atgatcgctc
      241 cgtgcgtctg gcacatcagt cacgatatca aacaaaaaat acacatcttt cttcattctg
      301 gtttttgttg ccggaatttt aaagtagttc ttattagagt aatagagatt gattaataag
      361 ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa atctttgaca
      421 aatctattcg aaggatcaaa tttaaaccat aattgtgttt ttccttccat caacataatg
      481 ttcacctcaa aaagagtata cgaactaatg ttctttttgt aaagtaggaa tatcattgat
      541 aatatggatg gaacatatat attaaaaagt gttctttttg aatgctattt tgaatgccat
      601 tttcattcat ttaacgaaaa tctgcgaaaa taaaaaatgc tcaaaccctt ataaaataag
      661 gatttaagca tctagcgaaa tctgctgaaa tctcacaatg gagacggcgg gagtcgaacc
      721 cgcgtccaaa cacatcgcca cttaaaaatc tacgttcata gtcaactcat ttaaggattc
      781 actttacagc ttgccgagtg acaggcattc tgtatcgcta gtctgatgtt ctctttttca
      841 acttacagac ggaaagttga aacgtatccc actaaagtta ggacccttac tcgagcacat
      901 gggcgatgcc gagaggatct acgctaactg tttttaggca gctaaagcgt aagagttgtt
      961 ttcgttttta gcagttatat ttaactgtaa cgtttttacg tagacgtaac ctacgaaacg
     1021 caattcaagc tcgactgtgc ctgtcgaatc cgtaacgtcc ccgttagcta acaagggtta
     1081 cgtgtatttt tcatacaaaa ttagtatagc atatattggg ataaattcaa taaaaaagcg
     1141 aatcatatcc cattatttat ataataactg cccctttctt aagaaaaaat aagcgatttt
     1201 cttaagaaaa gaggtagctt caaaaaaaga tcttaaatca gatcaaaata cttcaatgcg
     1261 tggacaattc cgccttctgt atttttcttt gtgataaagg tagaaagttc ttttaatccg
     1321 tcataggcgt tgcccatagc gattttgttg tcgcaagctt ccaacaaggc aaaatcattc
     1381 ggaccatcgc caaatccaaa agtaggaact ttttccagct gaagttcttt ttgcagacgt
     1441 ttgacacctg tcccttttga gattcctttc ttcactgtat cgatcgagta aggaccattc
     1501 cgataaaaag ttagctcagg aaaatgttcg tgatagtatt cgtcacctga ttgagataag
     1561 atcagcaaca tatttactgt gttttcttta aagcctaaag gatcaatttc cggaacatct
     1621 gaatgaatga agtcgtaagc attgatcatt gtttggctat gaccggtaca ccagattttc
     1681 tgatcgttat agtaagaaag ttcatgaccg ttcaactgta catgatcata cattttttga
     1741 cattcttctg aagaaaatgt ttgagaatag atttcttcgc cgtctactcg aatgaaagaa
     1801 ccattcatgg tgaccgcaga agtaatgccc gcatcttcca tgatagagtg gatttctgct
     1861 tctgtacgtc ctgtagcgat gacggggaga acattgtttt cttttaatgc gttcatagct
     1921 tttgccactt cgggtgtgat ttttgatttc gcatctagta atgttccatc aagatcaaag
     1981 aaagtgattg cacgataatt tgacacaaat aaaactcctt ttttattgga ataagtttat
     2041 cttaacaaag aatgaacaaa agacaaacct ttcgtgaaga attcctgaac atattggttc
     2101 agtcaacaat ttagagtata ctataagaag agaatttagg agggaaagaa tgattttttt
     2161 cagtattggt atcttattct taataattgg cagtcttttt cggattctgc cctcaaaagg
     2221 aaatctgcct ttttatgggt atcattcgcc tcttgccgca aaaacagacg ctcattggcg
     2281 gctggctcaa aagacaagcg gaaactggtt ttttcttatg ggcttactga tggcattgat
     2341 cggctattat ttaaagacat ctgggcatac gaattatttt ctgattgaaa tgcttctgct
     2401 agtatttcca attatgccta tttttattat gacagaaaag aaattacaga aatatgacct
     2461 ggaaactgga gggaacgata atgaatattt taatgattga agataatcaa tctgtttctg
     2521 aaatgatgca gatgtttttc ttaaatgaag gttgggaagc aactttcaag tatgatggaa
     2581 aagaagggct cgatgccttt ttagaaaacc cacaacattg ggatatgatt acactagacc
     2641 tgaatttgcc cacactggac ggtatgctag taggaagaga gatccgcaaa gtttcaagta
     2701 ctgtgccgat tatcatgctg acagctagag attctgaaag cgatcaggtg atcggcttag
     2761 agatgggggc agatgattat gtcacaaaac ctttcagtcc tctgacgctt attgctcgaa
     2821 tgaaagcttt gcatcgccgt agtgagttgg cagaagcaag agaagaagca gtagtttcag
     2881 atgatcattt tgatatcgag actagtcatt tcaagatgaa tacgaaaaca cgagaagcgt
     2941 atctggacaa ccggccaatt gaaggactga cgcctaaaga gttcgatcta ttgtttactt
     3001 tagcaaaaaa accacgtcaa gtattctctc gggagcagtt gcttgaatta gtctgggatt
     3061 atcagtactt tggtgatgaa cggacagtgg acgcccatat caagaaatta cgacaaaaaa
     3121 tcgaaaaggt cggaccacaa gtcatccaaa ctgtttgggg agtcggctac aaattcgatg
     3181 attctggtgt tgcctaatgc gttatttgta tcagcaactc ttagctttct gcggaatggt
     3241 cgcgttgatc ttattgatcg ttggagtttc ttttacacaa ctgacaaaac aaacgattga
     3301 agaaaacaat taccagcaac tgtttggata tgctgaatca gttgaaaaga caacgcaaac
     3361 ctatgccgat acgctcccgc agtttagtca agatcaggcg tttcaaaatg cattgctgct
     3421 gacagagcaa gtactgacag aacaaaatgt gaactttata tttatcgata aaaacgaacg
     3481 tgtcatttat ccgactaata cagctttact gaattttacc ttaacagctg atcaatggaa
     3541 aaatctgagg aatggacgtc aagaaaaatt cacatcgaat aaaaatattt taggacaagc
     3601 acaggcgacc tcttacgcac ttgtgccatt taatttgaat catgaattct atggagcact
     3661 tgtagttagt caacctgcgc gaaatatcga taacagcgtt cgtccaatta cactgaacct
     3721 gttcaaagga tttatttttt ccagtatcgt tgcagttatt gccagctatg cttttgcagc
     3781 attccaagtt aagcgtatca atcggttaag gaatgcgacg aaagaagtga cgaatggtaa
     3841 ttttgatgta cagctccccg tacatgacaa agatgaattt gatgagttag cagatgattt
     3901 caataaaatg acgaattcat tgaaagaatc tcaagcagaa attgaagaac aagaaaatcg
     3961 tcgaagacaa tttatggcag atgcttctca cgagatgaga acacctctta cgacaatcaa
     4021 cggacttctg gaagggttag aatacaatgc aatccctgaa aatcagcgag aaaacgcgat
     4081 taaattgatg aaaaatgaaa cagagcgtct gatccgtttg gtgaatgaaa acttggatta
     4141 tgaaaaaatc cgtacgaatc aaatcagtat ggtcattaaa aaattcaatg gaacagaaac
     4201 attgaaaaat attgttgcac agttagaagc taaagcagaa gcagcaggag atacattgac
     4261 attgaaagcc gttgatgata ttgatgttta cgctgattat gaccgatttg tccaaatcat
     4321 ggtcaatatt attcaaaacg cgatacagtt tactgaaaac ggccaaatca cgattacttt
     4381 agaaaaaggc tatcttgaaa cgatcatcac gattgaagat acgggaattg gaatgtctga
     4441 acaacaaatg aaaagtatct gggatcgtta ttataaagta gatccttcaa gaaaaaatac
     4501 aaaatatggt gaatcaggat taggtttacc aatcgtgcag caacttgtac gcttgcataa
     4561 aggaaaatta gaagtagaaa gcgaactagg aaaaggaaca aaattcgctg ttcgtttgcc
     4621 tgacgtagaa atcaaaaaag agtaaaaaac aagcggtata aaagatcatg aatcgatctt
     4681 ctataccgct tgtttttact ataaaatgat tgatttagcc cgcgggctct aaacgatcaa
     4741 tataaggact ttgagcaata gtagcatcat gagtaaagag taagatacct tgattttctt
     4801 catgagctaa ataagaaagc aagtggagta gctcttggat ctccaataca ctaagttcat
     4861 caaatgtatc ttcaataaca atattgtttc gcttggaaag caaggcatga agtaattgca
     4921 gctttacttt ttcaaaagac gtcaactcag ctgccgcaca attaagcaag gaaggagaga
     4981 tatcaagttt ttcttgatag gccaaacgat cttgtttgat ttctttttcc ggtacatcca
     5041 acaggacatg gtctttaaga ttcagataag gaaacatcgg ccaatttttt tggatgatcg
     5101 caaaattatc cagttttgca acaagaacct cttttttcaa gtgctccaat attttatgct
     5161 gtttttcgag aggacacaaa ataacggtca agtgttcagc atgaatcaaa tattcaggtt
     5221 tcattacgtc caggcctcct actttgcaaa tgatggataa aaaaagtgaa agggaataga
     5281 ctaagaatga gacagcatcc actgagccca ataagagttt gggcaaatcc cttaagtata
     5341 tctgtgaaaa aaagatcatt ttggaaatcg atggtaaaaa aagtctgttg atccatcgga
     5401 attgtaagtc cgcttaccga agtcaagata tcacggatat ctggcagttc agtagtcttt
     5461 aagacaagaa aattgatcct ttgaagaagc gcttcataaa ctgactggaa gcttacgatc
     5521 cacaataaaa ccaagataca acaaagaaag agaggaaaga acagctgcca gaaaataaaa
     5581 aggaataatt tggttttaga aagaccagtc aggtgccaat tgatcaattc ctgcttgttt
     5641 tgcctgactg aatagtaaaa gaaaaagata aaaaggaagg caaatagaaa aaataaactg
     5701 aatatgcctt ttatataaaa tcggctagct aattctaaag ccggaagaag ctctgtagat
     5761 gcagaagcgc caataaatga aagccgatct tggacttgat tggctaaagc cgattgcaaa
     5821 tggtaaagag tcaggcatgc tatcaataaa aaagcagaaa agcttgaaaa taaacaatag
     5881 agtattgtct gctttttgta ataaattaat cgagcagtag catagcggat tattttcata
     5941 cttttcacct ctataaaaag tatagaaaat gaatatgaat aaagtatgaa attcctttag
     6001 tgaaatcacg aattaaaacc agaaaatatt ttgtaaaaag caagaggttg tgaagaagct
     6061 gcccta
//
LOCUS       DADXTH010000114         6013 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.116, whole genome shotgun sequence.
ACCESSION   DADXTH010000114 DADXTH010000000
VERSION     DADXTH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.116"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            170..703
                     /locus_tag="IWI97_002572"
     CDS             170..703
                     /locus_tag="IWI97_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464041.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            715..918
                     /locus_tag="IWI97_002573"
     CDS             715..918
                     /locus_tag="IWI97_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464042.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            1240..1620
                     /locus_tag="IWI97_002574"
     CDS             1240..1620
                     /locus_tag="IWI97_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBC2464043.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1602..2516
                     /locus_tag="IWI97_002575"
     CDS             1602..2516
                     /locus_tag="IWI97_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBC2464044.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            2761..3450
                     /locus_tag="IWI97_002576"
     CDS             2761..3450
                     /locus_tag="IWI97_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464045.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(3571..3657)
                     /locus_tag="IWI97_002577"
     CDS             complement(3571..3657)
                     /locus_tag="IWI97_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBC2464046.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            4333..5283
                     /locus_tag="IWI97_002578"
     CDS             4333..5283
                     /locus_tag="IWI97_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBC2464047.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            5304..5837
                     /locus_tag="IWI97_002579"
     CDS             5304..5837
                     /locus_tag="IWI97_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBC2464048.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5853..>6013
                     /locus_tag="IWI97_002580"
     CDS             5853..>6013
                     /locus_tag="IWI97_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464049.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKET"
BASE COUNT         2202 a         1051 c         1126 g         1634 t
ORIGIN      
        1 acaagaattt aatataacat attttcctaa tgcattctaa ctataccaca ttatcaaaaa
       61 tataaattat ctttactaaa taaaatattg acaagcctat accttttgtt ttatgatttt
      121 cacaaagaaa acgttttcta tatatttttc tgtatcagga ggaaattcta tgaaaaaatt
      181 tactttaggg gtagctttgt taactattgg ggcagttgtt ttaccgtcaa ctactatagt
      241 ttatgcttca gaaaattccc aagtggtatc attaaacgaa aacaatgcta ctgaaaatgt
      301 agttattgag tacactgaag atggtatggt cgtaactaaa tatgaggatg gttatagtgc
      361 ggaagatttt gcaaattctt cagatatcat gacaagagct gcagttgcaa gatggggcaa
      421 aaaggaatat acaaatattg ctgtaaccac tggagtagct gctaatgcta ttaatgctgc
      481 cttttgggca ggagggaaag ctagcacttc tatgtttggt atcccaggtt gggcaattgg
      541 tgctttgtta acaggtgcaa gttggactaa aaaaggtgct tctccaggga atgcagttgc
      601 aaaagaatgg gacaaaaatg gtaatggctg gattggattt tattaccaaa ggggatatga
      661 tgcagctgga agagttgtag ctactagata ttcaacgtta taagttaagg agtgatgttt
      721 ttgatatcta aatcattaaa atcattaaaa acatttacta aaaagaatcc gcaaatagct
      781 actattcgta tttttatatt tgctatagct gttgctataa ttgttaatat gctaagcgga
      841 gggcaattat acaaatctgg tttattttta gtgttaatct tagttgtctt agacataata
      901 aagtctaaaa aaaaataaat cgatacattt tagaagaata gggttgtttg aatattacaa
      961 agcaacaatt tcaccttcgg tgaaataagc gaaactttac aaaaagtggc tagcaaatgc
     1021 tagccttttt ttgtattgtg gagcgtggat tttgaggggg attcaagggg gagcttgcgc
     1081 tcctttgaca agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc
     1141 ttgccaaacc cttttagtga gctttactca ttgtaccttt ttaggtacaa tggtattgcg
     1201 aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca acatcaaaat
     1261 ttggctagcg atcattctaa aaaatatacc tatcgatccg agccaaaaca aatcagtttt
     1321 cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt
     1381 gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg
     1441 ccagacattg cccaggcaat cgcgcgcgat ttagccaaag ctggagggaa tatcaatcaa
     1501 attgccaagt ggtgcaatac acaccaacac gacgttgctc ctgatgatgc tcaacgttta
     1561 tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca gttaaagtaa
     1621 gtcgaaccac ttcatgtagc cgagcaatca attacgcaga accacgagca accgtcaaaa
     1681 caggaataaa ctgtgacatt gattatgcca aaagtgaaat gaaacaaata cgcacgcttt
     1741 atggaaaaga cgatcgtgta caagcacact tattgattca gtcctttcga ccaggagaaa
     1801 ttaccgctga aaaagccaat caactagggg cagagtatgc gaaaaaaata gcccctaacc
     1861 accaaatcgc gatttacaca catacagaca aagaccatat tcataatcac attgtcatta
     1921 actccgttaa tcttgaaact gggaaaaaat ttcaagccca tgggcaagca tttttaaaca
     1981 aatgttatga cctaaatgac gagatttgtc ttgaacatga cttaacaatc acggaacgag
     2041 gaaagaaaac cgaaaaacga accatgtctg aaattaaatt aaaagaaaaa aatgaaccgg
     2101 tttggaaaga cgaaattcgg tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa
     2161 cgtataccca attttgtgat tcacttaaaa cattcggcat tcattgcttg aatcgcggaa
     2221 aaaattttac ttacgaattg gtccacaaaa agaaaaaagt acgatccaac aaactaggaa
     2281 aggattacga aaaggagacg attttacatg agctggacag acgagaacga acctacaatc
     2341 aacaacgaac caacgagcta cgaagagctc gagctagcat tcgcgaaact gcagaagaag
     2401 tacgaagaga acaacgaact tttacgcctg aacaatcgga aattagaccg actcgaagaa
     2461 accatgaaca acacgttgaa cgaggaacag accaccaaca agaattatcg ctctaaaacg
     2521 ttaaaagaca tcgaaaacga aataaagaga caacgtgcct ttcttgcgcc ttatacccaa
     2581 gaaaggatac aaactacgat ggattcacaa gtcaaaaaac tccaccaagc gctattagac
     2641 cagcgaaacc aagaacaaaa aaaggcttta attcaacgat cgatggatat tcttgccccc
     2701 ttatgttgct tagtactaac attattagcc ttttggttgg tatggggaga ataaacagac
     2761 atggcattct atttacccta tctactcatt tctgccagta ttttaggaat tatttggctt
     2821 agctataagg cttatcaaac aagatattca ttaaaaggct ctaaaattcg atttaagcga
     2881 tttttattgg tcggggttat atttgcctta tcaatcgttt taagtggttt attcggcgtt
     2941 ttagagggta ataaacgtgt ttcaagaagc attttattag gggatatcac tcaaaagtat
     3001 gaagcagcat gtgataaaaa gaaaaaagag caagcattag cacaaaaaat ggaggaattt
     3061 accgcttgtt atgaggacat gaacaatatt tttgtggatc aagagaaacg tttaacagat
     3121 aaaaacatga aaaaactcac tcgtttttat caaaacttac cagaaaaaca gcaaaaagag
     3181 gtacaagaaa aatatgagca agtaaaaaaa gacgtgcaat attttaaaga tacgcagaca
     3241 gaagaagctt gttgtaactt atttagcgat acaatccctt tttcaaccag tgaacaagaa
     3301 cgaaaagaac ggcaacaatc ggtcacatac gaacgttatg aaacactgct tcagcaagca
     3361 acaaacatac aaaatccaac gaaaaaagaa acggcattaa attatctaga aagtgtcaag
     3421 gaatggttgg atcagcaaca acaaaattga atttgattag caatcatgtt aaactgaacc
     3481 tgcaaatgat caaacattag atacacgaaa gccccagaga ggtcggattc tctggggctt
     3541 tttttcaggt tgatgtcgcc tttattgatt ttacttcttg tttcggttat tcaaccaata
     3601 actgaataaa gagacacaaa cttctacaag aagggggcaa atgaaccgtt caaacattag
     3661 atacacctcc ttccagtcgc aagactgtcg gataggcgac aagttattat accttacaaa
     3721 ataaaatctg tgtggtgttt ttaagtatcc cttattttcg ggttgttagg gcttgccctg
     3781 accgcctgta aggcgcttta agaacaagat aaggtttttt acccaagact gtcttaaaac
     3841 gacttagacg agctatcaga ggcttccccc acctccgtta ctgacttgtt tttagcaaag
     3901 gagcctttag gcgactgcgc agtacaatgt gagagcatag cgatcacact tttttagcga
     3961 ccgtagggag caaggttttg aattggcagt tttgaaaaat gcgcgaacct gttggtagaa
     4021 aaaagaagtg gtttttaggc gggaaatttt gtcaccctct tttataatct ctttttaaac
     4081 ctttttttaa acctctttac cgtcggctgt gtccttactc cttcaatgga tacagcgttt
     4141 tataaactac attttgtctg cttttaaact acattttgtc tgcttttaaa ctacattttg
     4201 tctgctttta aactacattt tgtctgcttt taaactcttt ttttgcactc acgtatgtta
     4261 taatatgcat tatcacagac aaaacgttgt ttataacgtc tgtatccatt gaaggagtaa
     4321 ggaggaatat ctttggcaaa tgaaatcgtg aaataccatc atgaattaaa taccattccg
     4381 ttgcggaaat ttacaccagt tgaaatgaat ctatttttct ctattgtgtc tagaatgcgc
     4441 gaaaaaggcg ataaaacagt ccgtcttact tttgaccaac taaaagattt aagtaattat
     4501 aaagcaactg ctaataatcg tttcattaat gatttagaaa tgacttatga taaattaatg
     4561 gatttacgtt ttggtaaaag aagtgctaat gggctcgata tagatagatt cgttatgttt
     4621 agtgagttta aaatacgtgg aactgctaaa gatccatatg tagacataca agtttacgaa
     4681 aaagctttac ctttactaaa caatttagat gagtgggttc gctattcact ccaacaattt
     4741 aacgaattac aaagtagcta tgcaaagact atgtttcgat tactaaaaca atatcgaact
     4801 aaaggttttg cttatttttc taaagaggat tttcatgaac tattagatat tccaaaaagt
     4861 tactggaaca accaagcaaa cgttgataat cgtgttataa agcctatacg acaggaatta
     4921 accgcaatct tcaaaggatt atcaatcaaa aagaaatacg gaaaagggcg aggaaaacct
     4981 gtcgttggct atcaatttac gtttaaacca gaaatcaaaa aagcagatga ttttaataaa
     5041 catgcaccaa ccaaagaaga acaaaataaa gcattggctt tagctgataa acaaaaagaa
     5101 tacgaagaac ctgctacaac aattaacgaa aaaatcaaac agaagaaacg agaacttgaa
     5161 ctcctccaac aaatcaaaca actagaaaaa gagaaagaag ctcttgaaaa acaaacagag
     5221 tccgcacttt cgaaaaaaga gaaaaaaagt ttattatctg atttagataa tttattccag
     5281 tagcaaccta ggaggcaacc agtatgcaac cacaagaaac ctttacaata aaggaattgt
     5341 cagaactttt taagatgagt aggcaagcaa tatcgaaaca tatccaaaaa ttagattctt
     5401 ccatgatagc taaaaatgag cgtggttata aggttgtatt gcgttctgga gtattgcaac
     5461 ttgctcgcaa cttaggcaac caaatgttgc tagataggtt gcacgagtac gaacaagaca
     5521 acgataaaaa tgttaatata ccaagcaaaa acgtggttac aaacaagttg ctagatcagt
     5581 tgcgtgaaga aaatgaattt ttacgagaac aacttgccct cagagagcaa aataattctc
     5641 atatacaaaa actactcgac cagcaacaac aactcaccct acaagccaac aaacaaatcg
     5701 aaaaactgca agaaaaacta cagttgactt atacagaaga agctccagag aatgactcaa
     5761 cgactctttc tgaagcaaca gaaacaatag acaaacaaat tgatactgaa aaaaaatggt
     5821 ggcaactttg gaaataactg gagggaacat tcatgacaag aaaaaatctt ctattcggtt
     5881 ctattttact aggaattcta ggattcagtc cgatgttatt gtcagtgatc aataaaggga
     5941 ataaacatgg tgaattgaaa ttgaatccaa attattacaa cagtcctaac gaagaattgt
     6001 ttgtaaaaga aac
//
LOCUS       DADXTH010000115         5936 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.117, whole genome shotgun sequence.
ACCESSION   DADXTH010000115 DADXTH010000000
VERSION     DADXTH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.117"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..742
                     /locus_tag="IWI97_002581"
     CDS             <1..742
                     /locus_tag="IWI97_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBC2464050.1"
                     /translation="IYSLLSEKEQENFDEYAYFVNTAIDRQSLGKVLGGKMISVVEPY
                     YEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAAHAAVAYLGALKGYKTIQ
                     EAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKTLNRHGNQKLSDEITRVG
                     SSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYRNLADPEAEQLRRMIVND
                     GLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            754..1185
                     /locus_tag="IWI97_002582"
     CDS             754..1185
                     /locus_tag="IWI97_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2464051.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            1199..2644
                     /locus_tag="IWI97_002583"
     CDS             1199..2644
                     /locus_tag="IWI97_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBC2464052.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            2930..4555
                     /locus_tag="IWI97_002584"
     CDS             2930..4555
                     /locus_tag="IWI97_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBC2464053.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            5187..5540
                     /locus_tag="IWI97_002585"
     CDS             5187..5540
                     /locus_tag="IWI97_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464054.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT         2071 a          818 c         1160 g         1887 t
ORIGIN      
        1 aatttattct ttgctgtcag aaaaagaaca agagaatttt gatgaatatg catattttgt
       61 gaatacggca atcgatcgac aatctttagg aaaagttctt ggaggaaaaa tgatctctgt
      121 tgtcgaacct tattatgaat ggataatgga tcgacagcag ttagatccaa ctacatcttt
      181 tgatatgaaa aatgtaaatt tggttgatga tatggagcct tacattgagc gaaagcttta
      241 tatcgttaat gctgctcatg cggcagtagc ttatttaggc gcactgaaag gatataagac
      301 gattcaagaa gcaatccaaa atatggagat tttcaatcta gtcaagcaat ttttaatgga
      361 gaatatagag tactttgtac aaaaatatca tttttctaga gaagaattaa catcttttgt
      421 ggaaaaaaca ttaaaccgtc atgggaatca gaaactctcc gatgaaatta ctcgtgtagg
      481 aagttcccct attcgaaaat taggccctaa tgatcgttta attgctccca tagtaaaact
      541 agattcatat catttacctt acgatgcagg aataaaagta attgctgcgg gatatcatta
      601 tcggaatctt gctgatccag aagctgaaca actacgtcga atgattgtaa atgatgggtt
      661 gaaagcaacg attaaaaaga tttctcgttt ggaaggtaag ctgctgaatg aagttgtcgc
      721 tgcttatgag tcccagattt agaggaggag aatatgctaa aagaacaatt gtcaggaaat
      781 acaatgttta tggatgcgat cgattcatgg caagaggcaa tccgagtaag tgcgcgtcct
      841 ttgataggaa agggaataat agaggaaagc tatgtacaag cgatgataca aaatgtgttg
      901 gataacggaa attatattat attactgcca ctagtagcaa tgcctcatgc gcgtccagaa
      961 tttgggagca aaggggtcgg gatgagcttt ttatgtttag ataagccagt catgttccca
     1021 agtgaagaac cagtaaaatt cttctttacc ttttcttctg attcgccaaa gggacattta
     1081 gacttaatgg ccaatcttgg agaattgctg agtgatgaaa aaatgtatca aaaattattc
     1141 gatgtgaaga cagaaaaagc attgttagat ttgataaaga agtaaaggag agaagaaaat
     1201 ggaggtagca aacaaaaaaa aacgagtctc catgcgagct aaagttcaag cgattggcgg
     1261 tttcttaaca aatatggtat tgccaaatat aggcgcattt attgcatggg gaattgttac
     1321 tgcattattc attccaactg ggtggttccc aaatgaatct ctgaatgaac tcgtaagtcc
     1381 gacagtcagc tatctattgc cgttactact tgcctatact ggtggtcgga tggttggtgg
     1441 tacacgtgga gctgttttag gtacaatcgg aacaataggt ttgattattg gtgctgacat
     1501 accaatgttc atgggagcaa tgattatggg gccacttggt gggtggatga tgaaaaaaac
     1561 agatgatttt ttagatggga aagttcctgc tggttttgaa atggtcgtca ataatttttc
     1621 tataggaatt attggtttca tactgatgtg cctttcttat ctggtagttg gaccaataat
     1681 ccaatcagct aatcaatttg taacgagtgc aattgaagca ttagtttcaa ctggtttttt
     1741 accgatactg tccttaatca acgaaccggc aaaagtctta tttttaaata atgttattga
     1801 ccaaggtgtt tattatccat taggtatgca acaaacatta gaggcaggca gatcaattta
     1861 tttcatggta gcatcaaatc ctggccctgg cttaggacta ttgttagctt attctttatt
     1921 tggtaaaaag gcagcaaaac agacagcacc aggtgcaatc atcatccatt ttttcggcgg
     1981 aatacatgag atttattttc catatgtatt aatgaaacca ctcactatat tatctatgat
     2041 tgctggtgga atgacaggaa tcatcacttt tgaattattt gatgcgggcc tggtagcagg
     2101 tccaagtcca ggttctattt tttcttattt agctctaacg cctcggggaa attttttggg
     2161 tgtcatggcg ggagtggtca cagcaacact ggtttccttt tttgtaacat cagtaatttt
     2221 aaaagcagat aaatcaacag atgaagacag aaattttgaa ctatcagttc aaaaatcgaa
     2281 aacgatgaaa tcagaaggaa gaaaattatt aaaagaagaa aatgctagag aaatatgtac
     2341 cgagaaaaaa gagatgaata aaatttcctt tgcttgtgat gcaggaatgg gaagcagcgc
     2401 aatgggcgca acaatattca agaaaaaatt gcaaaaagca ggtattactg atgttgagat
     2461 aaaaaattat cgaattgaag aggtaccaga agatagtgat ttggtgatcg tacatcaaga
     2521 tttggaggaa cggactagaa aagcacatcc taacatgaaa attgtgacat taaagaatta
     2581 tttgcaagat ccagtaatca atggattagc agaagaaatc attggattaa aaacagaaga
     2641 ctaatttatg ggaatttttg attaaccttt attaaaatta ttttgcctaa tataaaaagg
     2701 ttgtgacatg gcttgtgtcg caaccttttt ttgtcgcaca aataattaat cggatatttt
     2761 atgttcatat tttttgtgat caacgagaac tgatggggca aagatacatt ttagatactt
     2821 ttaactgtta atttacaaaa caaggtgaaa cgaatagaaa aatgaaaaaa tcgatacgga
     2881 agaatatgtt tgattaaaac atgcatgaag agaaggaggt tgatgataca tgaaaaaaat
     2941 atattcaaag gaaatcgatt gttttagagg cattgctgtt ttagcagtga tattttatca
     3001 tttgccaaat agtttgctgc caggcggatt gcaaggagta acgatctttt ttgtggtttc
     3061 tggtttttta atggctaaaa atacgataag agatgaaaaa aacagacaat tcagtttttt
     3121 acatttttac tataagcgta tcaaacgtat ttatccattt ttactgtttt ctttggtcgt
     3181 attcattttc atactaggga ttagcaacat tcgattgata ggaaacattc gtagtgagct
     3241 acccagtttg atttttggct ataacaattg gtggcagctg aatcaaggag tatcttattt
     3301 tgactcttac tttaatgctt ctttatttaa acattattgg tcgctttcag ttgaactaca
     3361 attctatttg atatggccat ttttgtttat tgtcattaag cgtatgagaa gaaaacaagt
     3421 gttttactta atctatacat tgatttttgt ttctattctt tttagtttgt ttttaccttc
     3481 aactaaggca tattatcata cgatagctaa actttttcca tttttattag gagtttgggg
     3541 gtattttaat cgaataacta ttggccgttt ctttgaacaa aacactttta gtaaaatctg
     3601 gcttttatta ctagctagtc tttgtctgat tctgttcccg atttttcctt atacactaaa
     3661 tgaactactg atcagtattt gttttgctct attgttagcc agtgtcgatg atatgaacat
     3721 tgcaaaaaag accaaaatcg ctagtacaaa actttctttt gtaggaaaaa tcagttatga
     3781 actttatttg gtgcatttcc cagtattgat tgctctgaca aatctattca aacatcacca
     3841 ttggaaatat ttggatatcg caggtctatt cactgtgttt ttatgtacat tactcatgat
     3901 tacttgcttt agaaaaggag tgcaaaagat gagatcaccc aaaatttgga cagctatggc
     3961 actaatgata atcggagttt ctgtactggt attacgtgct cctgccagtc gactgaccgc
     4021 tgaccaaaat cagttgcaaa gtttattaaa agaaaactct agtaaagcaa atgcaggaga
     4081 aaatctgttg tcacaagaga agaaaatatt atttatcggt gattctgtta tgctgggggc
     4141 gtatcaggaa ctaaaggaga catttaaaaa agaagcattg gtagatgcaa aagaatcaag
     4201 acaaatcact gcgttaccag aaatcttaaa ggagcataaa gatttagttt cttaccaaca
     4261 aatagtgatc gggttaggaa ccaatggtgt gataacagat gaagctatag aacaaacaat
     4321 gtctttgctg aaagataaaa aagtatactg ggtaaatata aaagcaccaa ctggctggca
     4381 agatacgata aatgccacat tagctacatt gccaaatcag tattctaata taacgatgat
     4441 cgattggtat agtgagagca aaaatcatcc tgaatttttt tatgatgatg aaacccattt
     4501 gaatgaaagt ggacgtacag cttatgcaaa gtatattgct tcagttattc aataaggtag
     4561 tgattacgtg tgttaacctt actttttcct aataggtgaa tcaatttaaa taaaaaatac
     4621 ttcatatgat aaacggtgtt taagaggagg agctttttaa tattcgtggt aaaaaagaga
     4681 tgggcgaggt cgttaactga gagcttcttg gaaagaaatc gctttggatt tatcctcttt
     4741 gttatacaga acatgagcga gacgacaata ctaggatgta tgttctgatc gaagttttat
     4801 ggttacaagc agcaatcagc ttagataaaa ttaaggatcg taggaaccaa gtaaagaagg
     4861 ctgttagtaa agaaacaaac gatcaagtat gttttactct taagaagatg cagtgaataa
     4921 ataatagtaa tataatgcta gaagggactg tgacaaaaga cgtgtcacag ccttttttgt
     4981 tattttaatt atcgtccagt ctttttttat aaatgattgc ttaagcatta aatattaaaa
     5041 aatatagagt tcaaaattac tgcacaatta gatgaaaaat ttgaacaaca aaaagtgtat
     5101 ttttatacta taatagcttt tgaatttagt tactctattc aaaagttatg ctatggacaa
     5161 gaaaaagaat aaatagaggt gagaaaatga atagtttcaa gaaaaaattt gtgagtagat
     5221 ttttaaatat gaaaaatttt tttgaaaaaa agggagatta tgatgcatat aattatgtat
     5281 taaatctaga aaagcaaaca tattatacac tacgtaaaaa cgatgataat gacttttgga
     5341 ctatgttacc taaaatgctt tcgattgact ctaagctttc ccttttagag gaatttatta
     5401 atttagacat tgaagaattt tgcgggggaa aagagttaat taatatggta gagagtgatt
     5461 atcataccta ttataaagaa agttgtggat acaaagtgga tgagcaatgt ccacaatcaa
     5521 ttatttttta cattgagtaa tatgaactat taagcaggaa agcaactaga agctactgat
     5581 ttagtattta caaatataaa ctatattgct aacaattatt ttctagaagg aataaaagaa
     5641 ctaagaatca agacgttatc ttgattctta gttcttttaa catggaaaag atggataatg
     5701 cactgttaat tcatgatgca tcatcagaaa agttggaagg agaagataga aatcattaaa
     5761 tgatttctac agatggtaaa tgaaaaaaca aaatattagc aaaaatttgc ctaaatagat
     5821 agtaacactt tctaaattaa atacaagcgc attcataaaa tgtttataca aatatctatg
     5881 tgataaaaat tggatatgtt cacaataaat aggaattaaa ataacgaatt tacgtt
//
LOCUS       DADXTH010000116         5863 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.118, whole genome shotgun sequence.
ACCESSION   DADXTH010000116 DADXTH010000000
VERSION     DADXTH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.118"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWI97_002586"
     CDS             complement(99..1229)
                     /locus_tag="IWI97_002586"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBC2464055.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="IWI97_002587"
     CDS             complement(1878..3080)
                     /locus_tag="IWI97_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2464056.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWI97_002588"
     CDS             complement(3140..3343)
                     /locus_tag="IWI97_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBC2464057.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWI97_002589"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWI97_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="IWI97_002590"
     CDS             4880..5692
                     /locus_tag="IWI97_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2464058.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1735 a         1210 c          938 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttc
//
LOCUS       DADXTH010000117         5740 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.119, whole genome shotgun sequence.
ACCESSION   DADXTH010000117 DADXTH010000000
VERSION     DADXTH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.119"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            221..655
                     /locus_tag="IWI97_002591"
     CDS             221..655
                     /locus_tag="IWI97_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBC2464059.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="IWI97_002592"
     CDS             678..998
                     /locus_tag="IWI97_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464060.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="IWI97_002593"
     CDS             1139..1330
                     /locus_tag="IWI97_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464061.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="IWI97_002594"
     CDS             complement(1401..1658)
                     /locus_tag="IWI97_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBC2464062.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="IWI97_002595"
     CDS             1816..2079
                     /locus_tag="IWI97_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464063.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="IWI97_002596"
     CDS             2103..2291
                     /locus_tag="IWI97_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464064.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2374..2559)
                     /locus_tag="IWI97_002597"
     CDS             complement(2374..2559)
                     /locus_tag="IWI97_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464065.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            2774..3145
                     /locus_tag="IWI97_002598"
     CDS             2774..3145
                     /locus_tag="IWI97_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBC2464066.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3295..3367)
                     /locus_tag="IWI97_002599"
     tRNA            complement(3295..3367)
                     /locus_tag="IWI97_002599"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Gln,seq:ctg)
     gene            complement(3413..3841)
                     /locus_tag="IWI97_002600"
     CDS             complement(3413..3841)
                     /locus_tag="IWI97_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464067.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(3901..4659)
                     /locus_tag="IWI97_002601"
     CDS             complement(3901..4659)
                     /locus_tag="IWI97_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBC2464068.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4954..5151
                     /locus_tag="IWI97_002602"
     CDS             4954..5151
                     /locus_tag="IWI97_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464069.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5257..5739
                     /locus_tag="IWI97_002603"
     CDS             5257..5739
                     /locus_tag="IWI97_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464070.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
BASE COUNT         1988 a          870 c         1134 g         1748 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgttgca gcagcattgt
     2341 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2401 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2461 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2521 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2581 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     2641 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     2701 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     2761 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     2821 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     2881 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     2941 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3001 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3061 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3121 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3181 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3241 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3301 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3361 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3421 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3481 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3541 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3601 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     3661 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     3721 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     3781 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     3841 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     3901 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     3961 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4021 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4081 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4141 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4201 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4261 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4321 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4381 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4441 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4501 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4561 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     4621 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     4681 gtttatcttt cctattataa atgtattttt attggaaatg gaagtattat attttaatat
     4741 gtaggaaata attagttttt aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa
     4801 tacaacagct gttttaattg tgaaaacgaa aacagtacgc tataatgcct gtaaggttga
     4861 tttttaactg taaataaaaa tcaatgagtt aaatctccaa agaaatagca gtggtaatgt
     4921 ggagaaaagt tccagcagtt taggaggatt ttgatgacgg aagaaatgat taatttagga
     4981 gaacaatatg catgcaaacc aattggtttt acaaaaacgg tcattggtga agttgtatca
     5041 aaaatgacaa actgcgccgt ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta
     5101 ttagatgaaa aagcaagcat ggttgttgca aaatatgata catttgaata aagaaaacaa
     5161 ttggacgtgc ggcgtgctgc aagtccaatt tctttgagaa ggtaaatcag tattcttttc
     5221 ctattttctg gagaagaaaa agcgaggcga gttgtcatgg taacattctt tacggttcta
     5281 ttgtttttat tcgttgttgt gatgttttta tttctttggc tggttcgaaa agaaattatt
     5341 tatcggactg tgcgaaatcg atgggtctac ttggtgatac ccttcttagt gtcactagtc
     5401 atttggtata cactcatcag tcatcctaca gcagatgaat tggcaaaagg gatcttgtca
     5461 gcaatgattt ttattagttt tttgttggac agtagaggaa tcacagaaga aggacttgtt
     5521 ttgaatagct ttgacaaaaa aggtgttcct ttttctgaga tcaataaaat tgtattatat
     5581 cagccaaaag gttctaaaat cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg
     5641 atgctgaaat tttctgcatc attagaagaa ttagttccat ttttgtccca acgcttaaac
     5701 gatgaggcag agattgatat tatgattgat ccagaataaa
//
LOCUS       DADXTH010000118         5712 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.120, whole genome shotgun sequence.
ACCESSION   DADXTH010000118 DADXTH010000000
VERSION     DADXTH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.120"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            114..1013
                     /gene="rsgA"
                     /locus_tag="IWI97_002604"
     CDS             114..1013
                     /gene="rsgA"
                     /locus_tag="IWI97_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBC2464071.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            1026..1676
                     /locus_tag="IWI97_002605"
     CDS             1026..1676
                     /locus_tag="IWI97_002605"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBC2464072.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            1677..2315
                     /locus_tag="IWI97_002606"
     CDS             1677..2315
                     /locus_tag="IWI97_002606"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBC2464073.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            2470..3324
                     /gene="mreC"
                     /locus_tag="IWI97_002607"
     CDS             2470..3324
                     /gene="mreC"
                     /locus_tag="IWI97_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBC2464074.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            3328..3837
                     /gene="mreD"
                     /locus_tag="IWI97_002608"
     CDS             3328..3837
                     /gene="mreD"
                     /locus_tag="IWI97_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBC2464075.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            4092..5666
                     /locus_tag="IWI97_002609"
     CDS             4092..5666
                     /locus_tag="IWI97_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBC2464076.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         1921 a         1092 c         1185 g         1514 t
ORIGIN      
        1 tcttttttgt gtttctatct cttttgatca attttcaagc catatacttt gagaagtttg
       61 catggaataa cacctacttt cctcttcgta tggtaaaata aacaaaaacg gaggtggcac
      121 ttatcaaagg acaaatcaga aaagcaatca gcggttttta ctatgtatac gcaaacggag
      181 agacctatca aacaagagga agaggaaatt tccggaatcg gaaaatcacg ccgcttgtcg
      241 gagatcaggt gatctttgag agtgaaaatc aaacagacgg ctatcttctg gaaatcttgc
      301 cgagaaaaaa tcaattagtt cgcccaccag tcacaaatgt tgatcttgga atcattgtca
      361 caagtatcgt agagcctgca ttttcttata atctacttga ccgctttcta gttaccttgg
      421 aatacgagtc gatcgaaccg attatttttt tgacaaagac cgacttgtca gacgacccag
      481 aaaaaatcga agagatcaag caaacctata ctaaaatagg ctatccggtg attgtaccca
      541 atcatacggg tgatacgaca gaactgatcc gttacttccc agaacggcta tcggtcttta
      601 tgggtcagtc tggagcagga aaatcgacgc tactgaatca aatatcaccg gaactgaatt
      661 tagcaacagg agagatttct gatgcacttg gaagaggacg gcatacaaca cgtcatgtgg
      721 aactgattcc tatctatggc gggttagtag cagatacacc aggatttagc tcgattgact
      781 ttttgacaat tgaagcagga gaactgccga gacaatttcc tgaatttgta gaagcttctg
      841 tccattgcaa atttagagag tgcatgcatg ctaaagaacc tggctgcgaa gtaaaacata
      901 gagtagaaac aggtggtatc gcacagacac gttacgacaa ttatttgcaa ttcttacaag
      961 aaatcgaaaa aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa taggaggaaa
     1021 caagaatgaa gatagcacca tccattttga gtgcagattt tgccaattta caaagagaca
     1081 ttgagttagt ggaaaaagga ggcgcagatt acatccacgt agatgtaatg gatggccaat
     1141 ttgtcccaaa tatcacatta gggccaaata ttgttcaggc aatccgtcct gttactaaat
     1201 taccgttaga cgtccatctg atgatcgttg atccagaaaa ctacattcca gcatttgcaa
     1261 aagctggagc agatatcatt actgtccacg tcgaagcaac tcctcatatt catcgggcac
     1321 ttcaaatgac aaaaagctta ggagttaaag ctggagtagt tatcaatcca ggaacaccag
     1381 tctccatgat caaacatgtt ttgcctttgg ctgatcaagt actagtcatg actgtcaatc
     1441 ctgggttcgg cggacaaagc tttattgaag aaacagtaga aaaaattacg gaactcgctg
     1501 atctacgtag agagaacaat tggacttatg caatcgaagt agatggcgga atcgttccag
     1561 aaacagcaaa aatctgtaaa gatgcagggg cggatgtctt cgttgcagga tcatatatct
     1621 atggagccga aaatccagtc ggacaaatcg aacgattaaa agaggcatta gactaaatga
     1681 aaattttatt agctgctgga ggtaatccgc aaaactggcc gcagtctctg ctgtccaaat
     1741 acgatgccta tgtcgggata gaccgtggtg cgctgtacct gcttcaagca ggctacccag
     1801 tagatctggc agtgggtgat tttgattcat tgtcgaacga agagcagaag gaagtatttg
     1861 caaaagccaa agagtatacc cgttcccctg cagaaaaaga cgatacagat acacagttag
     1921 gtatcttaaa aacatttgaa cgttacccgc aagcggaagt cactttgatt ggagctacgg
     1981 gcggaagatt ggatcatctt ttagccaatc tctggttagg tatggaacct cgcttcaaac
     2041 cctttgccca tcagcttatt atcagagatt gtcaaaatta tttgtcttat tatttgcctg
     2101 gagagcatat agtaagacag ataccagata tgaagtattt agcttattgc tgcttgactc
     2161 ccgtctcgaa tcttacatta aaaggcagca agtatgtatt ggaaaataaa gaagtagaat
     2221 atccgacatc ttatgcaagt aacgaatttc tttcagatac cgcagaattt tctttttcag
     2281 atggtatgat tgcagttatt caaagcaaag attgatccat aaacaaataa aaaaatttgt
     2341 ctttcaggct aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa gaggagcaac
     2401 ttaccttttg tttcacggca tttagtgata aaattgaaca caagagtcta attaattcga
     2461 ggtgtctttg tgaagaagtt caatcctaat aaaaatatta ttattacctt ggttattgtt
     2521 atcatcgtgg tgacgatact aagtgtgacc gctgcgcagc gagcgaatga aggaaagccc
     2581 aatttcttcc aatccatcgt taatgacagt gttgcaatgg ttgataaaac aatctctgcc
     2641 ccagtacggt gggtacgaaa tggagtagct tctgtccata atcttttttc aacttatgcc
     2701 gaaaatgagc ggctaaaaga aaaaatcgat agctatgatg acgtttcaca gcaaaacaag
     2761 aattacgaga gagaaatcga agcgttgaaa aaagaacttg agttgaatca gacgctgact
     2821 agttacgaaa aagtaacggc aaatgtcatt actcgttcgc ctgacagttg gcaggacatg
     2881 ctgatcgtag acaagggttc taaagacggt atcgaagcaa atatggcagt catgtcgcaa
     2941 aaaggattga ttggccgagt gatcgaggtc aatacggctt cgtctaaaat cgaattactg
     3001 tcatcctcta atgaaagctc caatcatttt ccagtacggg tatcttcggc taatggcgaa
     3061 gcgtttggtt tgcttaaaaa ctatgatgaa aagctccatg ccttagtggt gacccaatta
     3121 actggtgata cggatatcaa agaaggggat gttgtccaga catccggtct tggagggaat
     3181 tctccagcta acttgccgat cggtacggtt attaaaacga aaccagatag ttatgggctg
     3241 gatcgggaag tttatgtgaa accttatgca gaaatgtatg acgtgtcagt tgtgacgatt
     3301 gtccaacggc tagcggagga ttagtccatg ttgaagaaag aaacaatgaa gtactatctg
     3361 ccaatcgttt tgttcttttt gatgttgata gatggtcatt taacaagaat gctaggggag
     3421 tggtcgaaag gcacctatat gtcaaatgcc cactttctga tattggcatt attatgttgc
     3481 agtatggcgt ttgaaaaacg ttatttactg attaccacga ttgttctcgg ggctatctat
     3541 gacgcttact atattggcgt tatcggtatc tatgcagtag ctctcccttt aattgtatgg
     3601 ttgatgtatg taatgaaaga cgttatccat gtcaacatct ttactgaatt tttcagtatg
     3661 atcatctttg tcacgggtta tgaattgttt acgatggtgg tccagttgat ttttaaatta
     3721 gcagtagtaa ataacacgta ttttattaca aggtttttag gacctacact gctgttgaac
     3781 atgattatat ttgtattatt catttttccc tttaagaaat tattcagtga tgaataattt
     3841 ctttttttat ttttaggtcg aaagcgaaaa agaatcgttg ttatcttaag aaatgctaga
     3901 cttaacgcgg ctttttcatg atcattttct gaaacgaaca gttttgtcat ttttaaaagt
     3961 tgttttgaaa catttttgta acataactat tatatcgttg acacactgct atggtacaat
     4021 ttttatatcg ttaagaaaga cttaaatata tttagatatt tattaaatta agccagtcgg
     4081 aggaatgaag agtgaaaaag agtttaatat cagcagtaat ggtatgttca atgaccttga
     4141 ctgccgtagc atcaccgatc gctgccgcgg cggacgattt tgattctcag atacaacagc
     4201 aagatcaaaa gattgcagac ttgaaaaatc aacaagcaga tgcacaatca caaatcgatg
     4261 ccttagaaag ccaagtttct gaaatcaaca cacaagcaca agacctatta gcaaaacaag
     4321 atacattgcg tcaagaatct gcacaattag taaaagacat tgcagattta caagaacgta
     4381 ttgaaaaacg tgaagataca atccaaaaac aagcacgcga agcacaagta agcaacacaa
     4441 gctcaaacta catcgatgct gtattgaatg ctgattcatt agcagatgct attggacgcg
     4501 ttcaagcaat gacaacgatg gtaaaagcaa acaacgactt gatggaacaa caaaaacaag
     4561 ataaaaaagc tgtagaagac aaaaaagctg aaaacgatgc taaactaaaa gaattagctg
     4621 aaaaccaagc tgctttggaa tctcaaaaag gcgatctttt atctaaacaa gctgatttga
     4681 acgtactgaa aacttcatta gctgctgaac aagcaactgc tgaagacaag aaagctgact
     4741 tgaaccgtca aaaagcagaa gctgaagcag agcaagctcg tattcgtgaa caacaacgtc
     4801 tagcagaaca agctcgtcaa caagctgcac aagaaaaagc tgaaaaagaa gcacgcgaac
     4861 aagcagaggc tgaagcacaa gctactcaag cttcatctac tgcacaaagc tcagcaacag
     4921 aagaatcatc agctacacaa tcttcaatga ctgaagaatc atcatcagct acacaatctt
     4981 cagcaacaga agaatcaaca acacctgaat cttcaacaga agaatcaaca gcgcctgaat
     5041 cttcagcaac agaagaatca acaacagcgc ctgaatcttc agcaacagaa gaatcaacaa
     5101 cagtgcctga atcttcagca acagaagaat caacaacagt gcctgaatct tcaacaacag
     5161 aagaatcaac aacacctgcg cctacaacac catcaacaga tcaaagtgtt gatacaggaa
     5221 acggcacagg aagtagtact ccggctccaa cgccaacacc aacacctgaa caaccaaaac
     5281 ctgtaacacc tgctccagct ccttctggaa gtgtgaatgg tgcagcaatc gtagcagaag
     5341 cttacaaata tattggtact ccatatgttt ggggcggtaa agatccaagt ggctttgact
     5401 gctcagggtt cacacgctat gtttacatgc aagtaactgg tcgtgacatc ggtggttgga
     5461 cagttcctca agaatcagct ggaacaaaaa tttcagtttc tcaagcaaaa gctggagact
     5521 tgttattctg ggggtcacaa ggcggaactt accacgtagc gattgcttta ggcggaggac
     5581 aatatatcca tgctcctcaa ccaggcgaaa gcgtaaaagt tggttcagtt caatggtttg
     5641 cccctgactt tgctgtcagc atgtaagaat gattaatcaa aaagagttag cccattctgg
     5701 actaactctt tt
//
LOCUS       DADXTH010000119         5617 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.121, whole genome shotgun sequence.
ACCESSION   DADXTH010000119 DADXTH010000000
VERSION     DADXTH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.121"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(113..388)
                     /locus_tag="IWI97_002610"
     CDS             complement(113..388)
                     /locus_tag="IWI97_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBC2464077.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(360..1313)
                     /locus_tag="IWI97_002611"
     CDS             complement(360..1313)
                     /locus_tag="IWI97_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBC2464078.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1925..3418
                     /locus_tag="IWI97_002612"
     CDS             1925..3418
                     /locus_tag="IWI97_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBC2464079.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3553..3849
                     /locus_tag="IWI97_002613"
     CDS             3553..3849
                     /locus_tag="IWI97_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBC2464080.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(3873..4832)
                     /locus_tag="IWI97_002614"
     CDS             complement(3873..4832)
                     /locus_tag="IWI97_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2464081.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5015..>5617
                     /locus_tag="IWI97_002615"
     CDS             5015..>5617
                     /locus_tag="IWI97_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464082.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT         1830 a          930 c         1016 g         1841 t
ORIGIN      
        1 atgttgtata cccccaaaca atacacatat tttgaaggat taggattaat aaagctaaaa
       61 atatagtaag tatactaaat atatttacta tatctagtat acttactata ttttaatctt
      121 ttgatcgtct attatatata atccgttcct cgtcgtttaa agtatcaaca taggcgtcca
      181 ttgcaagttt gactatttca tacataggaa tatcttttgc ataactaaga tcccgaatgg
      241 tttttagtac gggaggatct acttgaactg gttttctttc tttcgcagtg tactttcgtt
      301 cccctaactc gacattaata tcatctttaa ttgaaatatc ttcagcacca attttcttct
      361 tatctttgtt cgttggtaat aatgccattt agtttaatct cctttccaag aggtaggata
      421 tgattggcca ttgagtcagc atatttaggt gtataatcaa atccttcaat atcaccatgt
      481 tgttcatagt attcaattct ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac
      541 catcttcgat ctacataatc atttaattgg ataccattct caccatacat ttctaaccga
      601 tccgagcctt taacaatgct tttaaaaatg tttgattcac catacttatc aattgttttt
      661 tgatagtttg cgtgttgaga ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg
      721 atttgaagat gagactcttc aaaattcgtg acaatcggaa ctaaaacttt attaataaaa
      781 tcttctgttc cttccattgc gtaccgtttt aattcttgca ttgcaataat ataatctgca
      841 gcagttaaga atgcacgaac cacattatct gttgatggtg ggcagtcgat aaagatataa
      901 tcgaaatttt ttctcaattc ttctaaaggt ttaatgaagg ccaatgatct cttacgacga
      961 tcaggatatt tttcggacaa gtattcatta agttcttcag atccaggaga gccagccata
     1021 atatataaat gtggacttac ttgagtaata cctgaaaccc agtccccttt tttgattgct
     1081 gcagacatgg atcgatcaaa gctagtaatt tgatacgttg atccaatgtt ggaagtagta
     1141 tttctttgtt ggtcataatc aattagcaac acgtcttttt caaaaagttg actagaaaca
     1201 atcgctaata aatcattgtc tgtagtctta ccaatgccgc cttttctacc tgtaagcatt
     1261 acaacttttc ctagtttatc tgatgtttta aatgcagtcc gtaagttttc catagttccc
     1321 tccaatttcc tatactaaat atatttagta tacttcatat acttaatata ttcgatatat
     1381 ctaatatact ccaaattgat aagaattcag actttataag gaatatagtg gatatactaa
     1441 atatatttgc tatattcagt atatcagata tattcaaaat gtcaaaatat atttgtgcgt
     1501 tttgcgttga caatttagag tgaaaagagg taaacttaat tctatagaaa ccacagataa
     1561 ttcactattt acaaagtagc aaaagcagtg gtactcttta aacagaaaaa ttacataaaa
     1621 aaaagagacg gaaataattg attttagcag aatcaattat ttctgaatca catggttagg
     1681 acatgtgttc acgctcgtct caagaatatg taagtagcta ccaactactt atattaaaag
     1741 ttgatcggat accttttccg attgccttta ttctaccaaa aaaaggcgag caactcaaca
     1801 gaagaacgtt tatttagtgt acttaaatta aatattctta aacactgaag agagaacaca
     1861 tactatggct gagattaaag ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca
     1921 atcgatgttg agcgaaattt attcagctat tcttaaaaac ggcttacgta catacaaata
     1981 tcgaaacagc accatgaaac ctgcaggtca tattgattcc agtaggagtg gtgctatctt
     2041 tgcgtaccgt agcaagcaat taatgaatgt tgggcgtggt atggtcatta cttcggaaga
     2101 agctatttta gagaacgaaa agaaactaac gcattggaca cctaatacct atcgctttgg
     2161 tacttatgcg gatgattatc gaacagtggt taaaggtcat tctgagaaaa acttaagtca
     2221 aatcaacaca tttgtagttg atatcgattc aaaagagaac catcaaggcg aaattatttt
     2281 agcctgttta gatcaagtgg gctacatgcc aacgctaatt ttagaatcag atcatggtta
     2341 tcaagtttat tttgtactaa agacaccagg ctatgtcaca aagaaaagca atttcaaggt
     2401 gatcgatgta gcaaaaaaaa tttcaaaaac aattcgtcta cagttggctg aaaaattgtc
     2461 cggagtggat ctaggatgta accacttcgg aattgctcga ttcccacaag aaaataatat
     2521 tgtttttaaa gaaatgaact atcagtattc ttttgctgac tggattcaat ggtcgatgaa
     2581 gcttgaatca gatcaaaaaa caaaagaaga tcgagaacga aaactaattg tctttcctga
     2641 gaaaaaagaa attcgtcaag tagatgaacc gtggttcgat atgttacttc ataaagcaaa
     2701 catcgtcggc ggagaaggac gtcttggtcg aaataatgtg attttcacgc tttctttagc
     2761 ttattactct tcaggttatg cattggatac ttgcgaatac aatatgttca cattcaacga
     2821 acggttacag gaaccgctat ctgaagcaga attagtaaag attgtacgta gtgcttattc
     2881 tggtaattat caagcagcaa gcagagaatt cattattgaa ttgtgccagc aatgggtttc
     2941 accaacctta aaaaataatg atctgttcat tcaacgacgt ggctggtgga aatttaaaaa
     3001 gccacgagat caaagaaaat atagtcataa aaatgaatgg aaagaagatc tattggctta
     3061 tctaagcgaa aaaagtcttg ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc
     3121 ttcacaacta acgatgccca agcgaacact tgataagatc ttaaatgagt tacgagagga
     3181 gaataaagtc ttctacaagg taacacgagg aagaaatggc ggaatgatca ttgcttcagt
     3241 caaaacgttg ttcgcgaggg ttattcaact aagtaaagaa aagagactag attattcaac
     3301 aaggatcaca gaggtatttg gactcaacga gagcttagta gaagagacat ttagacaatt
     3361 atcaaccccc ttgaaccctc ttaaagaagt gaatcttttt gaagtagata caggttgaaa
     3421 aataaaaggt gcaaattgcc ttatacaata tatctatgat acttctgtag gtgttgctcc
     3481 ctttgcctag ctacgtgtag gtggtatact tataactgcg tatgcagtct tatgtatcat
     3541 ggaggaagtt gtatgtcgtt aaaaaattat gtctattcac atcctgttaa tgtgtttatc
     3601 attggtcgtt tgggattatc tgtggaagtg ttctgtgagc tttatggttt taagcagggg
     3661 actttgtctt cttgggttac cagggaaaaa acagttgctt ctttgccgat tgagtttctt
     3721 catgggttgt ccttagcttc tggggagact atggatcagg tatatgattg tttgtgtgtg
     3781 ttagagcagg agtatattga gtataaaata gctaacgagt taagaaaaag gaagaagtat
     3841 atccagtagt agtttttatt tactcttaat tgtcaaatta agttagacaa atcatcaata
     3901 cttacgtaac tcaaaaatac ttccaaaggt gttcgataat tcagtgattt tctaggaata
     3961 ttatttcgtt tcgatgcgac agattgaata aaagattcat ctactgaatt gaaatccata
     4021 gatttcaata aaccatctct acgtaacaat ccgttagaat tctcgtttag gcctctttga
     4081 gacggcgttc ctggatcagc gaaataaatg gcaatatcat tggcattact gatctgtttc
     4141 caatttgaaa attcctttcc acaatcaaaa gtgatggatt tgaatagatt tttcggtaca
     4201 gattcaaacc aatgatttaa tttgttttca atatcaatcg cttgtctacc acacggtttc
     4261 aatgtgatga taacttttga taatcgctca actaaggtaa ttacagcact tttatgtttc
     4321 aaacctacga tagtgtcacc ttcaaggtga ccaaactcat ttgagaattg gctataatct
     4381 ttttcacgtt catgaataga gcgacggaaa gcttgttttc cacgtctttc ttgatgtcca
     4441 tttggtttac gcttgccttt catcggtaag tcagaaggat taaatagccc ctttttgaac
     4501 atacgataaa tggtacgagc agaacaacga ataggaaacg ctgcacgacc aacaatcaca
     4561 tcgggtgtcc atccttgaac aacctttctt tgaatatatt ctgattgttc ctcaggtaaa
     4621 acaagcgggc gtctaccaca gtttgatttg tttttcttat actgttgata gtactctaag
     4681 gctgattttc cttgcttgaa gaacaggtat actttatgga tcgtttgtct tgaacgattc
     4741 aagcaatgcg cagttttcgc aacagattga tttattttga aataagactc tatcattaca
     4801 agttcatccg ttgtaagatg ggtataggtc atttgtactc actccttata attctttgga
     4861 cggaactatt ttgagtgtag cacaaatgac ttttttagtt gtctagctta attttacaat
     4921 cggcgtactt aaaaaattgg ttcattgagc aacctatcgc tgctaaagta gcaaaggaaa
     4981 acgttaaagg aaagaattta gggaagaaat cgaaatgaaa tcggacgact ggattgaaca
     5041 cacctggctt cctctgaaaa gaaatagcaa acgaaagaaa ttttgggtat tgcaattatg
     5101 gccattagga aaactttgta gttacggggc acgacttatt cttgatttaa ccgtttactc
     5161 gattggagta tttggaacgg cttattactt gataaatgat cccaatagga ttgcagcatc
     5221 agacattttt acagtgctaa tgacgttgat ttttgtcatg ctattgtcta tttttactca
     5281 attagtcatg caatttacca ggcaaaaatt aacagcagga aaacttttca aaagcctatt
     5341 cgatttaatt gttgtaaatg caattctagc gactttaata aaacaaattg aacaatctgg
     5401 aagaacaaca aaaggagtga aggatttttt attgacgttt attcactttg taaatggaca
     5461 catgtatttg tttgaagtgg gtattattct cttttttgtc gggtatgtat ccacgaaact
     5521 tattaaacgt tcaattgaaa aagaaattga aattgacctg attccgacac ttcttggtta
     5581 tgagtacaat gttgattttg gaagatatga cgaattg
//
LOCUS       DADXTH010000120         5072 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.122, whole genome shotgun sequence.
ACCESSION   DADXTH010000120 DADXTH010000000
VERSION     DADXTH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.122"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            129..353
                     /locus_tag="IWI97_002616"
     CDS             129..353
                     /locus_tag="IWI97_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBC2464083.1"
                     /translation="MMKYLQIVVIDGMNCSRCASKVRNALEAEPTVSVKIVDLARGTV
                     TFDADSKLTLTFLNQLLKNTKYTVTRDISI"
     gene            364..1947
                     /locus_tag="IWI97_002617"
     CDS             364..1947
                     /locus_tag="IWI97_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HBC2464084.1"
                     /translation="MWGNQHKISTYFFYGNYIIDMGDDGLKNNKYIWLAATISMLTLG
                     GVFILIQQQNKEEHAEVVFQEEQRQISSRMSNMMGDNQEADLKVVHQTERQLLIPPVL
                     EPSSKSKENVIYDIVAQNGEVQIMDGEKTETLGYNGDFLGPVIRLKKGQKVTINTNNN
                     LDASTSFHWHGLKVASDADGGPHQIIEAGQKKSVTFEVDQEASTLWFHPHPEGETASQ
                     VYKGLAGLMYIDDDNSKSLDLPSKYGVDDIPLIVQDKSFSSTNQINYENDFNSDGTKG
                     ETLLTNGTINPYIEIKNRWMRYRIVNGSNSRNFTFTLDKDESFYQIATDGGFLNTSVK
                     LSKLLLAPGERAEILVDTQNYKKGEVIHLLANNLVALTMRIENTIENKEFTPSDSLNT
                     ISTLDEKKLEDLARQSINLRGMSHMVNINNKQFDMERIDLYKKLGTQEIWEVNNISSM
                     MGGMIHPFHIHGVQFQILSRDGNQPALNEQGWKDTVLVNPDETVELLVKFDREGIFMY
                     HCHILEHEEYGMMGQMEIK"
     gene            1975..2655
                     /locus_tag="IWI97_002618"
     CDS             1975..2655
                     /locus_tag="IWI97_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2464085.1"
                     /translation="MKILIVDDEEKILEIIDAYLVANHYSVYKAISGAMALEKFEKNN
                     PDLVVLDLMLPDTDGLTVCRKIREVSNVPIIMLTAKSDEDDILTGLKLGADDYMIKPF
                     SPKELVARIQTVLRRTETLSNPNKLSSSNGELIVYPESRQVYLHQVELNLTTTEFDIL
                     HALISTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHIKNLRHKIENDAKSPMYILTVHG
                     TGYRFGGH"
     gene            2655..4049
                     /locus_tag="IWI97_002619"
     CDS             2655..4049
                     /locus_tag="IWI97_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2464086.1"
                     /translation="MRKRTIKTQLVLSFLAIATLIIGSISLVALSLTNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKALENNIYFSIEDEQGNMVWELKGK
                     NLKSAQDKLKKNALKVSEKKSVKLDETIEAKKKLINDGNEFGKVTFYYFGPFAYTEHD
                     ALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIPQ
                     ETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGVW
                     KITPERLQSCYDEIDRLTRLIGNIEVINKIEAKYDHLNKTEFNIYKLIESVIENFASK
                     IESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVLL
                     IVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVVEAHKGSITVKSK
                     LGLGTKFVIKLPFQ"
     gene            4064..4270
                     /locus_tag="IWI97_002620"
     CDS             4064..4270
                     /locus_tag="IWI97_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464087.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            4291..4887
                     /locus_tag="IWI97_002621"
     CDS             4291..4887
                     /locus_tag="IWI97_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HBC2464088.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSKSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1870 a          701 c         1007 g         1494 t
ORIGIN      
        1 ctctaaaaca aactctatac atggtttctc cataaatttt ccttatgata cttgtaagac
       61 aattcttaaa agattgatta gattagtaaa tccaatatag gcgacttttt taagaaagaa
      121 ggaattttat gatgaaatat ttgcaaatag tggttattga cggaatgaat tgctcaaggt
      181 gtgcatcaaa agtaagaaat gctttggaag cagagccaac agtcagtgta aaaatagttg
      241 atttggctag aggtacagtt actttcgatg ctgattcaaa attaacttta acattcttaa
      301 atcaactact aaaaaataca aagtatactg taactaggga tatatctata taagtataag
      361 tatatgtggg ggaatcaaca caaaatctcc acatattttt tttatggtaa ctacataata
      421 gatatgggag atgatgggtt gaagaataat aaatatattt ggttagcagc aacaatatca
      481 atgttgaccc taggcggtgt tttcatattg attcaacagc agaacaagga ggagcatgct
      541 gaagttgtct ttcaagaaga acaacgacaa atctcaagta gaatgtccaa tatgatgggg
      601 gataatcaag aagctgattt gaaggtagta caccaaacgg agagacaact attgattccc
      661 cctgttcttg aaccatcttc aaaaagcaaa gaaaatgtga tatatgatat agttgctcaa
      721 aacggagaag ttcaaataat ggatggagaa aaaactgaaa cattaggcta taacggggat
      781 tttttaggac cagttattag actgaaaaaa ggtcaaaaag ttactatcaa tacaaacaat
      841 aatcttgatg ctagcacatc ttttcattgg cacggtttga aagtagcttc tgatgcagat
      901 ggaggtccac atcagattat agaagcagga cagaaaaaaa gtgtaacatt cgaggttgat
      961 caagaagctt ctacactctg gtttcatccc catccggaag gagaaacagc atctcaagtt
     1021 tataagggtt tagcaggttt gatgtatatt gatgatgaca actcaaaatc attagattta
     1081 ccttccaaat atggtgtcga cgacattccc ttgattgtgc aagataaatc tttcagctcc
     1141 actaatcaaa ttaactatga aaatgatttt aactctgatg gaacaaaagg ggagacgttg
     1201 cttactaatg gcactatcaa tccatatatt gagataaaaa accgatggat gaggtataga
     1261 attgttaatg gatctaattc aagaaacttc acttttactt tggataaaga tgaatcattc
     1321 taccaaattg ctacagatgg tggttttctg aatacttctg ttaaacttag taaactactt
     1381 ttagcccccg gggaaagggc agaaatatta gtggatactc agaattataa aaaaggtgag
     1441 gtaattcatt tactagcaaa taatttggtt gccttgacaa tgagaattga gaatacgata
     1501 gaaaacaaag agtttactcc ctctgatagt ctaaatacta ttagtacttt agatgaaaag
     1561 aaattggaag atttagcaag acaaagtatt aatttaagag gaatgtctca catggtaaac
     1621 atcaacaata agcaatttga tatggaaaga atagatttat ataaaaaatt aggtactcaa
     1681 gagatttggg aagttaacaa tattagtagt atgatgggag gcatgattca tccgttccat
     1741 atccatggtg ttcagtttca aattttatct agagatggaa atcagcccgc actaaacgag
     1801 caaggatgga aagatactgt gctggtaaat ccggatgaaa cagtagaact tcttgtgaaa
     1861 ttcgaccgtg aaggtatttt catgtatcat tgtcatatat tagaacatga agagtatgga
     1921 atgatgggac aaatggaaat taaatagaaa ataatttaag aaggatgatt gtctatgaaa
     1981 attcttattg tggacgatga agaaaaaata ttagaaatta ttgatgccta tctagttgct
     2041 aatcattatt cagtttataa agcaataagt ggcgcaatgg cattggaaaa gttcgagaaa
     2101 aataatcctg atttagttgt gttagattta atgcttccgg atactgatgg attgactgtg
     2161 tgtagaaaaa tcagagaagt atcaaatgta ccaattataa tgttaacagc taagtctgat
     2221 gaggatgata ttcttactgg tctcaagtta ggtgccgatg actatatgat taaaccattt
     2281 agtcccaaag aattagttgc gagaatacaa actgtattgc gcagaactga gaccttatca
     2341 aatcctaata agctttcttc aagtaatggg gaattgatag tatatcctga aagtcgccaa
     2401 gtttatctac atcaagtgga attaaacctc acaacaactg aatttgatat tctacacgcc
     2461 ctaatatcca cacctaataa agtcttttct cgtagtgatt tgatagagaa agttaaggga
     2521 atagaatttg atggactaga taggagcatc gattctcata taaaaaactt aagacataaa
     2581 attgagaatg atgctaaatc acctatgtat attttgacag tccatggtac aggatataga
     2641 tttggtggcc actaatgaga aaaagaacga taaaaactca attagttctt tcctttttag
     2701 caatcgcaac attaataata ggatcgatta gtttagtcgc attaagtttg acgaacaatc
     2761 attttagtaa atacgtagaa gagcgacaag aagatttgtt gaatcagtat gtatatacaa
     2821 ttgatttatt gtggttaaat agtggagaaa catggaacag cgaggaactt gcagcacttt
     2881 ctgagaaagc attagaaaat aatatttatt tttctataga agacgaacaa ggcaacatgg
     2941 tttgggaact taaaggaaaa aatttgaagt cagcacagga caaactgaaa aaaaatgcct
     3001 tgaaagtttc tgagaaaaaa agtgtgaaac tagacgaaac aatagaagcg aaaaagaagt
     3061 taattaatga tggaaacgag tttggaaaag taacgtttta ttatttcgga ccttttgctt
     3121 atactgagca cgatgccttg tttatctcca gtatgaaaca aagtttaatg tatgttgcta
     3181 ttgcagctct tcttgtatca tttatacttg cttcgtggat ctctgctaga ttaggattac
     3241 cactaaagca tgtaagcgac tttacacata aattaacccg tggggaatat gctgataaaa
     3301 tcccacaaga aacttcaatt attgaaatta attccttgat tgattcccta aatgatttaa
     3361 gtaatcagtt ggagaaacag catggacttc gaaaaagatt aactactgat atttcacatg
     3421 aattaagaac tccattagct acccttaaag gcaatgtgga aggaatgatt gacggagtct
     3481 ggaaaataac tcccgagcgc ctacaatctt gttatgatga aatagatcgt ctaactcgtt
     3541 taattggaaa tatagaagta atcaacaaaa ttgaagcaaa atatgatcat ttaaataaaa
     3601 ctgagtttaa catatataag ttgattgaat ccgttattga aaatttcgcc agcaaaattg
     3661 agtcaaaaaa ccttcatgtt gaaatccaag gggataacat caatatatct gcagataaag
     3721 ataaaatgaa ccaagttgtt atcaatcttt tgaataatgc tattaaattt acgcaaaagg
     3781 agggaaccat taagttttct ataagtaaaa ataaagacca tgttctttta attgttgaag
     3841 acaacggcat cggtatagaa aaagatcagc aacttcatat ttttgataga ttttatatgg
     3901 ctgacccctc cagaagtaga gctttagggg ggcaaggtat cggattagct attgtaaaga
     3961 gcgttgttga agcgcataaa ggtagtataa ctgtaaaaag taaattaggt ctaggaacta
     4021 aatttgttat aaagttacca tttcaataaa ggaggagagt tatttgtttc aaaaacagat
     4081 aaaaattgct aaactagtat gtgaatgttg ggcaaaaaaa atattttttc aactgatttc
     4141 gatagatagt gtcgaaaaag ttcatttaga tatcttatca aattctgtag taatcttttc
     4201 taagaaggac ttatcaatag ctacaattaa aaaagaagtt tttggattac aaatttatga
     4261 gatgacctaa atgtgattaa ttaaaggagg atgaagacag tgaaaaagat gacaaaatta
     4321 gtatttgcaa gtcttggaat tattgtattt ttaggagcgt gtagcaatca aagtgaatcg
     4381 aataattcta aaagcacaaa cgaggaatcg acttctacag cgtctagtga aatgaatagt
     4441 atggagggaa tgaatcacga aggtatggtt ccttcttcaa tgaaagatgc tgcgaaccct
     4501 aaattcccgg tgggaagtaa tgttatactg ctaggcgatc atatggaggg gatgaaaggt
     4561 gcaaatgctc aagttgtagg ggcctttgat actacaatgt atgaagtaag ctatgagcca
     4621 aaaactgggg gaccaatggt taacaatcat cgatgggtag tacaagaaga gttaaaagat
     4681 actgagactg tagcaaatga gggcgatact gttatcttga atgccgatca catggatggg
     4741 atgatgggag ctgaagcaaa agtagataaa tctattacag gaactgttta cgttgttaat
     4801 tacactccaa ccgatggtca agaagaagtt aagaatcata tgtgggtaac tgaagatgag
     4861 atggaatatg acgaaaataa tgagtaattt tatatactaa accaataagc tggtacatgg
     4921 ccttcgtatt ctcaacatga ggaatatgag ctctttgtta ataaggactg atgagttgtg
     4981 caaaatcaaa tctgggtcag aacgaacctc attctggctc agatttttgg ctcttcgaca
     5041 tttagtgctg gtagaaagac aactgaaaat tt
//
LOCUS       DADXTH010000121         4791 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.123, whole genome shotgun sequence.
ACCESSION   DADXTH010000121 DADXTH010000000
VERSION     DADXTH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.123"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(75..1073)
                     /locus_tag="IWI97_002622"
     CDS             complement(75..1073)
                     /locus_tag="IWI97_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBC2464089.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="IWI97_002623"
     CDS             complement(1148..1444)
                     /locus_tag="IWI97_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBC2464090.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="IWI97_002624"
     CDS             complement(1437..1730)
                     /locus_tag="IWI97_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464091.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="IWI97_002625"
     CDS             complement(1876..2427)
                     /locus_tag="IWI97_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBC2464092.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="IWI97_002626"
     CDS             complement(2601..2900)
                     /locus_tag="IWI97_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBC2464093.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3193)
                     /locus_tag="IWI97_002627"
     CDS             complement(2927..3193)
                     /locus_tag="IWI97_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBC2464094.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            complement(3215..3373)
                     /locus_tag="IWI97_002628"
     CDS             complement(3215..3373)
                     /locus_tag="IWI97_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464095.1"
                     /translation="MKNFNNCVVLIIITELTLFTNIYYIHEAQSSLIEDWAIFMAKEG
                     KEMFQNKG"
     gene            3540..4223
                     /locus_tag="IWI97_002629"
     CDS             3540..4223
                     /locus_tag="IWI97_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464096.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4260..4757)
                     /locus_tag="IWI97_002630"
     CDS             complement(4260..4757)
                     /locus_tag="IWI97_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2464097.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1428 a          912 c          696 g         1755 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3241 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3421 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3481 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3541 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3601 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3661 aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3721 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3781 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3841 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3901 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3961 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     4021 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4081 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4141 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4201 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4261 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aaaatgtttg
     4321 atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg atttttttca
     4381 taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt cgcttcatcg
     4441 agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag ccgttgaatc
     4501 tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg atgatcctta
     4561 ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc agttgtcaat
     4621 tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa cgattcataa
     4681 aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa atacgctagt
     4741 gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa t
//
LOCUS       DADXTH010000122         4666 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.124, whole genome shotgun sequence.
ACCESSION   DADXTH010000122 DADXTH010000000
VERSION     DADXTH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.124"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..110
                     /locus_tag="IWI97_002631"
     CDS             <1..110
                     /locus_tag="IWI97_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HBC2464098.1"
                     /translation="RYFRRLNRQHRVVYKVDEDVHVVEIYSARTHYESQ"
     gene            187..516
                     /locus_tag="IWI97_002632"
     CDS             187..516
                     /locus_tag="IWI97_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBC2464099.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            506..793
                     /locus_tag="IWI97_002633"
     CDS             506..793
                     /locus_tag="IWI97_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBC2464100.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGGLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1079..1936
                     /locus_tag="IWI97_002634"
     CDS             1079..1936
                     /locus_tag="IWI97_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBC2464101.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1937..2485
                     /locus_tag="IWI97_002635"
     CDS             1937..2485
                     /locus_tag="IWI97_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464102.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2745..3473
                     /locus_tag="IWI97_002636"
     CDS             2745..3473
                     /locus_tag="IWI97_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBC2464103.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWSKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLRYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            3503..4630
                     /locus_tag="IWI97_002637"
     CDS             3503..4630
                     /locus_tag="IWI97_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBC2464104.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEEAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYSKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIEHVKKIMSFSKKNLDI"
BASE COUNT         1689 a          618 c          970 g         1389 t
ORIGIN      
        1 gtaggtattt tagaagattg aatagacaac accgggttgt ttataaggta gatgaagatg
       61 tacacgtggt tgaaatttat tcagcaagga cgcattacga gtcacagtaa ttacttgtaa
      121 atacattatc tcgtggtata atgtatttac taagtaatta catactgtta gaaatgaggg
      181 atttaaatga ttacaaaaac aagaaaacaa ggcaattcaa tcatgttgac agtacctaaa
      241 gattttaatg ttcctaatgg agtcgaagtt gaagcaaagt tggtagagaa tggtattctt
      301 tatgaatttg ttgaaccaca aaaagaattt tatgatttta gcgaagatat tttgtctgat
      361 ataattgcag aaggctacga taaagatgag attcttgtag aatttaagaa tagaaaaaac
      421 aaaatgcatt cttcctttag agatattgct gaagatacac ttactaattc taaagtaatg
      481 actaaagagg agttggcaac agaaattggc ttatgaaatc gttccttcaa aacatgtcat
      541 taagtattta aaaaagctaa aagagaagcc attgaaagaa aaatttttga atttaattta
      601 tgatgaaatt gcgattgatc catataaggg tgaacaaaaa actgggggtt tatcaggtat
      661 ttggtctagt gggttcaaat atgcgggcac aacatataga gtagcttacg agattataga
      721 aaacaaggtt attccagtat tgttatgtgg aacgcatgaa aacttttatg aacagttgaa
      781 aaaattaatc tagtgatttg ttgtcgttct taagaaggac ggcttctttt ttatcgaaaa
      841 tctgattcgt ctacatgact aaagtcgcga gtattctctg cttctcgata aattaaataa
      901 cgtcattact ttgaggaaag ctataaatca atatatttca gcttttcctt atttatgtac
      961 taccttttac ttattaatta taaggtagta catatatatc ttttttttgg tagtaactaa
     1021 aaataaatat gttataatag tatcaaatga atattgctaa aagaaaagag gtaggaacgt
     1081 ggtagcgaag aaatatgtaa tcggtaattt taaaggtggc gtaggtaaaa gcacgtgcgc
     1141 gcaaatgtta gggtttgaat cagcagttaa tgaaggaaaa agaaccttga taattgactt
     1201 ggatatgcaa ggaaatactt ctgatgtgat gaacttaacg catatgaact tcactaacga
     1261 agaaggaggc ggagaaggtg aaccgattgt ttacgaaaat actgtgactg acgtgttgat
     1321 aaacaatctt ccacctaaag aggccgtcta taaagtaatc aataacctct atatacttcc
     1381 agcagacatg tcctttgaaa tgtatgatga ttggatcaaa gaccaatata atgattcatt
     1441 atcccaattc aaatatatga aagaaaagct agaaccatta tttgatgagt ttgacgtcat
     1501 ctatttagat gttccacctt ctatttctgt atatagtaaa tccgcgatgt acattgcgga
     1561 ctgggcaatc gtcgtcttgc agacccaagt aaaatcgatg agaaatgcga tgcaatatct
     1621 tgagtatatg gacttttttg tgaaagaatt tgatacaaat ttatatgttg ctggtgttat
     1681 tcctttcatg ttggaaagcg gtgacgcagt agatcaggaa atgtataaac aagcccaaga
     1741 gatttatggg gaacatttga ttaaaaatgt tgtactcaaa aatgcgcgct taaaacgata
     1801 cgacggatca ggtattacga tggagaagac aaagaaaggt ctcttaaaac aatgggatcg
     1861 caaaagccac gaattattta tcagtatttt gaaagaattg gaagaacacg agaattggta
     1921 tgcggaggtg gagtaattgg ctgacatcaa aggacttgat atttttggga acaacaaagg
     1981 actcgccaaa ataaaaaaag aagcagaagc aaagaaaaag cagcaagcaa ttacaccatc
     2041 tgaaaataag caattagaag tgcaccaagt tcaagaaata aaaacttcct ccaatgttga
     2101 aagacagagt agaggaagaa caaaaaaagt tggcgcacca atcagaaagt ttgataaggt
     2161 ttattccacc aagcatcctt taaaactgtc gactttgtta aatgcgactt ccagagtttt
     2221 agtagaaaag tatgagacta atatgacaag agatgaattg cttagaaaag ctttagatga
     2281 gtatattaaa cagaatctta cgcaagaaga taagcaagat ttgtttaatg acgtggtgaa
     2341 agaactagac ttgttcagag aaaaatatcc aattgttcca gaattagatg atcgtggaga
     2401 aataataaga actaccagtc agataaaaca agataccgaa gactcactgc gcaaagggtg
     2461 gggaatgaat aataaagtta gataataatc aaagaagaac taagtaacta cctttaaacg
     2521 gtagttactt agttcttctt tgatttgcgt gaaatagagg ggatttattt ttctggttac
     2581 gtgattaaaa aatccaaaaa atgagcatag gaacggttat gattttaggg tgtgttttaa
     2641 tgagatattg tgatttcatt aacaagtgta tgataatctg ttgttgagtt agtaaataag
     2701 taatagagct aactctaaaa taaaactatt aggaggagat gtaggtgaaa aagtttaaaa
     2761 agaaaatata tttaggattg gttgttgggg tgagtatgtt tgggttcatg gctcatacta
     2821 aacaagtaac tgcagatact aaccaaaaag aaaggattat gagccaatta gatgactctc
     2881 aaatagagta tgtacccaat gagctaattg tcagtttaaa ggctacacaa agaatttcaa
     2941 cagagaaaaa taaaccgcaa aatttattgg gtgtaaaagt tatggtggaa aaagatttga
     3001 ctcttaggac aaatgaaagc gattataata agcgagatca agtattatta gtgaaattga
     3061 acgaagaaat tagtgtggaa gaggcagtaa gaacatttaa tgcttctgat atggtcaatt
     3121 atgcagagcc aaactatgtg gttgaactag agccgttgaa tgatgttgag gagccaaata
     3181 caactgattc tttaggaaat cgatcaatta ttcttaacga tcctaattgg agtaaacaag
     3241 ctaatttaag agagattagc atgccatccg cttgggaaga aaccaagggt tctaaggatg
     3301 tgaaggtagc ggtcattgat tcaggaattg attatcaaca tgaagatctt tctggaaatg
     3361 tagatgaaag tttgagatac gatttttttg acgatgatac agatcctatg gatactatgg
     3421 gacatggaca catattgccg ggattattgg tgctcaaaca aacaacgaat taggtgttgc
     3481 tggaataaat tgggatgtta cgatggtacc tatacgtgta tttggtagta gtggaggtac
     3541 cagtttggca atcttaactg aagctattaa ttatgcatcc caaaaagggt ttccaattat
     3601 caattttagt ataggtagtg ttgtttatag cagtacagaa gaagcaatta aaaactatcc
     3661 tggattattc gtagcagcag ctggaaataa tgggatagat atgactgata aaccttttta
     3721 cccagcttct ttggatatac ctaatattat tactgtagga aattctagtg gttatgaacg
     3781 aagttctgat tcaaattata gtaaaacagg tgttgattta ttcgctagag ggtttcgtgt
     3841 ttttagtaca tttctaggaa gtaaatacac gtatatgtca ggtacctcga tggcaacacc
     3901 tcatgtcact ggtagtgctg cattagcgtt gtcactagat cctgatctat ctactgatga
     3961 attaaaaaaa atcattttaa atagtgttga ttcattttat gaactatctg atttatgtct
     4021 tacaggggga agactaaata cgaataatgt tgtacgacaa gtaacgggta aacttactgc
     4081 atctaatatc acattaagat taaatagtga atggtcagat gaaattgcta aagagatgtg
     4141 tcacgttcac tggattgacc agtcaggaaa tgatttgacg gatcaagtgg ttgttctaaa
     4201 aaacgaagtg gatacatcta actttggcga ttacgaagta gtatttgcat cacctgattt
     4261 aacagatcaa atctcagtta cagtgaccgt acctcgatta cgtaaactta cgctaggaaa
     4321 agaaacagca accattgata ttggttccgc atgggatagt gtcatagcca ttgatacttt
     4381 tcaagtgaaa gctatagatg attttgggaa tgatgttaca aatcaagtac aggttactat
     4441 taatgctgat atagatacaa ttaattcaat tcaagcagtg attaagtttg aagttccaga
     4501 attaggctta gcccaaattg gtagactgaa tattactgca aaaaatagta caatcaatgg
     4561 attgaatgaa gttagcatcg agcacgtaaa aaagattatg tcgttttcga agaagaattt
     4621 ggacatttag agggtgatac gattgtcggc atccaccata aaagtg
//
LOCUS       DADXTH010000123         4317 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.125, whole genome shotgun sequence.
ACCESSION   DADXTH010000123 DADXTH010000000
VERSION     DADXTH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.125"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            165..1943
                     /gene="uvrC"
                     /locus_tag="IWI97_002638"
     CDS             165..1943
                     /gene="uvrC"
                     /locus_tag="IWI97_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBC2464105.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            2088..2987
                     /locus_tag="IWI97_002639"
     CDS             2088..2987
                     /locus_tag="IWI97_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2464106.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            2980..4242
                     /locus_tag="IWI97_002640"
     CDS             2980..4242
                     /locus_tag="IWI97_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2464107.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
BASE COUNT         1470 a          702 c          920 g         1225 t
ORIGIN      
        1 aagctgaggg tgggactgaa tccagttccg tcctcggttt ttttgcggtt gagttaaggt
       61 tattcaaatt aatatttttt gagtataatt acgttgtcag gctggattgt ttttttgaca
      121 gacatgctat aataacttta attgaatatg aaggggagta ccagatgaac gaacgaatca
      181 aaaacaaatt agctttactt cctgatcagc ccggctgtta cttgatgaaa gataaaaatg
      241 gaacgatcat ttatgtcggc aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta
      301 ctggcagcca taatacgaaa acggaacggc ttgttagtga aatcgaagac tttgaataca
      361 tcgttacaga atcgaatatc gaagcattgc tcttagagat caatctgata aagaaaaatg
      421 atcctaaata caacattatg ttaaaggatg ataaaaccta tcctttcctt aagataacga
      481 atgaaaaata tccccgtttg gtgattactc gtaaagtact aaaagacaaa gcactgtatt
      541 ttggaccata tcccgatgtc ggtgcggcga atgagacaaa gaagatcctc gatcgattat
      601 ttcctctgag aaaatgcaaa cctagtcaga cgaaggagcc ttgtctttac tatcatttgg
      661 gacaatgtct gtgtccttat tatttcgacg tagatccgaa agtctatacg aaaatcgttg
      721 atgaagtgaa aaagttttta aacggaggac acgaaaaaat cgagtcagag atccatcaga
      781 aaatggaaaa agctgctgaa aacatggagt ttgaaaaagc tgcagaatac cgagaccaga
      841 tccgtgccat tgagacaatc atgactcgcc aaaaaatgac aaatactgat ttactggatc
      901 gagatgtatt tggttatgcg attgacaaag gatggatgtg tgtccaagtg tttttcgtaa
      961 gacaaggaaa gctaattgaa cgagatgttt caatgtttcc tttctataac gaagcagaag
     1021 aagatttctt gacttacctc ggtcagtttt atcaggaaaa tgagcatttc attcctaaag
     1081 aagtcttgat tcctgatacg atcgacaagc ccagtgtaga agctttgtta gctacgaaag
     1141 tgctgcaacc aaaacgagga gaaaagaaaa aattagttga attggcgaat aagaatgcct
     1201 cagttgcttt gaatgaacgg tttgatctga ttgccagaaa acaggaacgg acgatcggag
     1261 cagttgaacg tttaggaaac gcgatgaata tcccgacccc aatacgtatc gagtcttttg
     1321 ataactctaa tatcatgggg acgaatcctg tggctgcgat ggtcgtattc attgatggaa
     1381 aaccggcaag aaaagaatat cggaaatata aaataaaaac agtcaacgga ccggatgatt
     1441 atgcatcaat gagagaagtc atctatcgca gatattcacg agtgattcgc gaagggttgc
     1501 ctttacctga tttgatcttg atcgatggag gaaaaggaca agtagatgtg gcaaaagatg
     1561 tcttagaaaa tcaattagga atcgatattc ctattgctgg catggcaaaa aacgataaac
     1621 ataaaacaaa tgagttgcta ttcggtatag atttggctgt agtaccgctt gagcgaaatt
     1681 caccagagtt tttcttactc cagcgaatcc aagatgaagt ccatcgattc gcaatcactt
     1741 tccatcggca gctacgcagt aaaaacagtt ttgcttcccg attagatgaa attgaagggt
     1801 taggaccaaa acgaaaaaaa atgctgctta aagagttcaa atcgttgaaa aatatcacag
     1861 cagccacgac agaagaatta caagaaatcg gactgccgaa aaatgtcgca caaaacgtat
     1921 ttgataaatt acatcaggaa tgaggaaagg gcactttaca aagaaagtaa agtgtcctat
     1981 cattttatgt acaacaaaaa gagcgtttac gttttgttgg taaataataa atcgcttacg
     2041 tctggtgtct aaggatatat gtgatttctt gaagaaggag tgaagaaatg ttagaagtca
     2101 aggatcttgt taaaactttt gggaactaca cagctgtcga tcatgtctct ttccaaatcc
     2161 ccgatggaaa aatcatgggg ctaattggcc aaaacggggc tgggaagaca acgacttttc
     2221 gtttgatctt ggattttttg acacaagacc aaggagaagt gttatggaat ggccatcatc
     2281 ttggggagaa agattatgat gtcatcggtt atcttccgga ggaacgggga ctttatccga
     2341 aagtaacgat tcaagaccaa ctgctttatt ttgcagaact tagagggaaa tcaagaaaag
     2401 agattgaacc aaaaatcgat ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg
     2461 ataaagtaaa atctttgtct aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa
     2521 tccatgaacc taaattgatc attttggatg agccattcag cgggttagat ccggtaaatg
     2581 ctgaattgct aaaagatgga atattggaat tgaaaaaaca tggttcatgt gtcatttttt
     2641 ctagccacaa tatggataat gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg
     2701 gaaaaatggt tttgaatgga aaagtacatg agattcgtga atcatttggc cgtacgaagt
     2761 tattcttgga atctggttta agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag
     2821 tgatccttca tgaagacaat acgttagaga ttaccttaga agatcctgaa gctgggaaag
     2881 aaatctttac tcgtgcgact cagttcggct acattcctat gtttaaccag caaccgccaa
     2941 ctttggaaga gattttcaaa ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt
     3001 accaaagatg tctatttaaa aaatgttaag tcgatttctt tcttgatcat gatattagtc
     3061 ccttttatca tgctgggtat catttatgta gcaggaaact ttgctcaaca aaatagtgat
     3121 gtcaataaaa tcggcgtatt ggctgataat gagcaaatca gtcagcaaat gagccaagtc
     3181 aaaacagatg attttgagtt caaagcagtt gattctgaag aaaaagcaca aaaagagctt
     3241 tcggaagaaa aaatcgatgc gtatttagta gtctcaactg agaatgataa gataaatggg
     3301 atgctttata gcgaaaattc attaggacaa agtacacaac tactgataca acaacaactg
     3361 actggcttgc aatcgatgct tcgagcaagt caattaggca tttcaccaga agaagtagct
     3421 agcttgagcc aacaagcaag tttgactcga caaaaagtca attttgattc aaaaggaaaa
     3481 atgacagttg gagaagataa ctcgacagtc caatatgtcg taagttatgt agcaacgatt
     3541 gtcttgttca ttattatttt gacgtatgcg cagatcattg cacaagagat cgcttctgaa
     3601 aaaggcacgc ggatcatgga agtgatcctg tccagtacga ctgcacaaaa gcatttttac
     3661 ggaaaactaa caggtgtttt acttgttgca ttaacgcaaa tggcattata cggtattatt
     3721 tttggtatag gatacagtca attcaaagat atggaaatgg tcaaaaatat attagacggt
     3781 atctcattgg ataacttgat tggaccattc ttgtggtatt ctcttctatt catgatcttt
     3841 ggcattttga ttttttctgt tttagcagct ttatgtggct ctttggtcaa caaagcagaa
     3901 gatacagcaa aagcaatttt gccagtgact tatctatctc taggcggata tatgcttgga
     3961 ttgatactag gagcttcaga tccaaacaat atcgttattc ggatcacttc gtatattcca
     4021 ttcttatctt catatattat gccggttcgc ttagcaaatg agacagtcgg tatgggtgga
     4081 gcattgattt ccttgttggt acttgtagcc gtaacattct tattgatgat cttgtctgca
     4141 aacatgtaca aatcgaatgt actcgtgtat agcgaaggcg gattatggag ttcattgaaa
     4201 caatcgattt caattatgag aaatgaacga aaaaaagcat aaaatataga aaaatataga
     4261 aggaagctgg acatttattg ttcccagctt cttttttgta tagacctatt tctattt
//
LOCUS       DADXTH010000124         4207 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.126, whole genome shotgun sequence.
ACCESSION   DADXTH010000124 DADXTH010000000
VERSION     DADXTH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.126"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            32..256
                     /locus_tag="IWI97_002641"
     CDS             32..256
                     /locus_tag="IWI97_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2464108.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            271..1140
                     /locus_tag="IWI97_002642"
     CDS             271..1140
                     /locus_tag="IWI97_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBC2464109.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1121..1855
                     /locus_tag="IWI97_002643"
     CDS             1121..1855
                     /locus_tag="IWI97_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2464110.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1888..2796
                     /locus_tag="IWI97_002644"
     CDS             1888..2796
                     /locus_tag="IWI97_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBC2464111.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2793..3335
                     /gene="sat4"
                     /locus_tag="IWI97_002645"
     CDS             2793..3335
                     /gene="sat4"
                     /locus_tag="IWI97_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBC2464112.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3428..>4207
                     /locus_tag="IWI97_002646"
                     /note="aph(3')-IIIa"
     CDS             3428..>4207
                     /locus_tag="IWI97_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBC2464113.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILL"
BASE COUNT         1442 a          627 c          967 g         1171 t
ORIGIN      
        1 aatattctta gcgtaaactt ggaggtgctg tatgactacg gcagaaatga ttaaagaact
       61 gtgtgagcaa atgaatataa gtgtttccga acttgctaga cgtattggcc agactccaca
      121 gaatttcaat aaaaaattac aacgagaaac agtaaccttg gatgagttga aggccattgc
      181 tgatgtgcta ggtgtcaagt ttgtgcaggc atttatttta ccagatggag aagaaataaa
      241 aatatctaac gaataaagga ggatgtttat atggtaggta atgttattca aatagtaaca
      301 gagaaattat catctttgcc ttttatagaa ggcatcgtat tagggggctc acgtgcaaga
      361 ggcacccata cagaaaattc tgatatagat atcggaatct attacaattc agactcattt
      421 gacctgacag cgattaatca aattgctaca gaattggatg ataaaaatag aaacaacctt
      481 gttgtgcctc ccggagcgtg gggtgattgg attaatggcg gcggatggtt atttataaat
      541 gggtatcatg ttgacttgat tttacgtgat ataaaacggg tagaacaaat aatcaaagat
      601 acggagcaag gaattgttac tgccaattat cagaccgggc atccccatgg ttatataagt
      661 gcaatgtatc gtggagaatt agcgattagt aaaatactat atgctaagaa cgaaagctta
      721 tgcgaattaa aaaagcaggc agaaatttac cctactgctc taaagaaaag cttgatgaac
      781 ttttttatat ttgaagctga attctcttta atgtttgtaa aagcaaatgc gggggtagag
      841 gataaatatt atattgcagg ccatgttttt cgtattattt catgcttaaa tcaagtacta
      901 tttgcatgta ataatgctta ttgcattaac gaaaagaaag ctataaaact gcttgaaact
      961 tttgaatata aacctgaaaa atatgccgaa agagttaatc atatttttga agtactcggt
     1021 ctttctcttt ttgaatgcta cgatatgacc gagaaacttt ataaagaagt gaatgaaatt
     1081 gtatcggaga taaataactt tttaaacgag gagagttcag atgaaagaaa acaaatatga
     1141 tgataatata ttttttcaaa aatacagtca aatgagtcgc tcgcagaaag gactggctgg
     1201 tgcgggagaa tgggagactt tgaaaaagat gctacctgat tttaagggta agcgtgtgct
     1261 tgatttagga tgcggctatg gatggcactg tatatatgcg atggaaaacg gtgcttcctc
     1321 tgtagtaggt gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt
     1381 tccacagatt gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt
     1441 tgatgtaata ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa
     1501 aaagatatat aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt
     1561 ttttactgct catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt
     1621 cccggtggac aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt
     1681 tacaaaatat catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat
     1741 aataaatcag attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc
     1801 ggatgaaatg cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata
     1861 tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta
     1921 ctttctttag cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca
     1981 aatattaata tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat
     2041 atagaaccgt ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg
     2101 caaaagccgg aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg
     2161 ctatttgatg attacaataa aattgatctt accttattgc ccttggaaga gttagataat
     2221 tacctaaagg gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg
     2281 gacatagttc cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat
     2341 gattgctgca atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag
     2401 gaaattttat ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg
     2461 atatcatgga aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag
     2521 tttattgaaa ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg
     2581 gattcctatg aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta
     2641 tccggtgagg tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca
     2701 aaatatacca gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca
     2761 tatgccgctg atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc
     2821 tgaaagatat cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
     2881 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
     2941 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
     3001 ctgataagat aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc
     3061 gaaaaaattg gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg
     3121 ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact
     3181 tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata
     3241 attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
     3301 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
     3361 tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa
     3421 ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
     3481 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
     3541 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
     3601 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
     3661 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
     3721 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
     3781 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
     3841 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
     3901 ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
     3961 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
     4021 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
     4081 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt
     4141 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
     4201 tttactg
//
LOCUS       DADXTH010000125         4174 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.127, whole genome shotgun sequence.
ACCESSION   DADXTH010000125 DADXTH010000000
VERSION     DADXTH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.127"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            124..558
                     /locus_tag="IWI97_002647"
     CDS             124..558
                     /locus_tag="IWI97_002647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464114.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            1848..2771
                     /locus_tag="IWI97_002648"
     CDS             1848..2771
                     /locus_tag="IWI97_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBC2464115.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            2787..3326
                     /locus_tag="IWI97_002649"
     CDS             2787..3326
                     /locus_tag="IWI97_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBC2464116.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1548 a          622 c          707 g         1297 t
ORIGIN      
        1 actaaccttt aaaataacac gttttataaa ataaaccctc ttgacaatat ttaattttct
       61 gattattcta aatatataca gaaagcgctt tcctaggatt ttatatctaa ggaggaagta
      121 acaatgaaaa ataaaattat taaaattatt acttgtctat ttttgttcgg tatgattgtt
      181 ggagcaagta ctccgttaaa tctgtttcct tttagtgcta gaggtataaa agtggaagct
      241 gcaagttcac gatataatca taatcatcgt ggatttactt gctataaaat gaactattat
      301 gttactaata aaatgtgtag agatttgaaa aaaaattata ataagctaaa gactcctgct
      361 caaattgcat cttttattcc tattggtgga gttggaacat gggttattac aaatgctttt
      421 agtagtgctg ttgatgcaat gaatgtcttt gtaagagctg caaatcaagg aaaaggtgta
      481 caattaacat ataatgctca ctttagcaat acaacatcat atcaatacaa tgattacgct
      541 cgttatgtaa taaaataatc agaaagtgaa actatctcat gaataaaaag attttagctg
      601 taattatttc aataatagtt ttaatcacta tgttcttcat atttaggcta attttcaata
      661 taagcttaca aaaaagtatt ctttatctaa ttcctatagc atttatctta gcaattttta
      721 gaagtattta cggaaataaa aactctaaat agctaaaaat aaatcatact aaattttaca
      781 aaaagtggct agcattttct agccactttt tgtattgtgg agcgtgggtt ttgaggggga
      841 ttcaaggggg agcttgcgcc cctttgacaa gcggttattt gcaccaaata cggaggtatt
      901 tggctagcaa atatagagct tgccaaaccc ttttagtgag ttttgtagaa agtaccttta
      961 aaggtacttt cacattacga acgatacgtc cgtaggacgt actgaaaggg tgggacttat
     1021 gagcaaatta atgcaaaatt tggctagcaa aaaagctaaa aaatatatgc taataatcga
     1081 gcgaaataaa cactttttca agtgagcgaa tccgccatga caaagctgaa gcagtttatt
     1141 tcagctttgt catggcgtgt gccgactttt ttaaaagtct aggcacacga gcaaatatct
     1201 ttatcaagct ctaatttgcg ttttaagacg tttgaaacgc ctaagtatat aaatatataa
     1261 gaataggttt aagagtgctt aaatcgaatt ttagagggca tttagacaat tttagggttg
     1321 ttagggcttg ccctgaccgc ctgtaaggcg ttttaagaac aagataaggg atttatacta
     1381 ggactgtctt aaaatgtctt aaacgaacgc tcagagcttc tcaaagggtt ccttatgaac
     1441 ggcttttagc aaaggagccg gaggcgactg cgcagtataa tgtgagagca gagcgatcac
     1501 acctttttag cgaaccgtag ggagcaaggt ttggaattgg cagttttgat aaattcgcga
     1561 acctgtttag agaaaaaagg agtgattttt taggcgggaa aattagcctc ccctcttttt
     1621 atatctcttt ttatatctct ttttatatct cttttatgtc ggctgtgtcc ttactctccc
     1681 aagcaattca gagttttttc aagtaggttt tctacgcttt tcaagtaggt tttctacgct
     1741 tttcaagtag gttttctacg cttttcaagt aggttttccg acattattga gtatttttat
     1801 aaagtatgtt acttttattt catactttat aaaagtagga gaagcttatg gcaaatgaaa
     1861 ttgtaaaata tgaaaataga ttaaatcata ttcctctgag aaaatttaat agtcgtgaaa
     1921 tgaacttatt tttttcaatt gcttcacgtg tacgtgaaaa aggtgtagat gagattattt
     1981 tttcatttca cgatttgaaa aaactaagcg gttatacaga acgtggggaa caatttataa
     2041 aagatcttag tagcacgtat gacaaattat taagtattaa tgcttggaat gatgatggaa
     2101 atactttaac taaatttgtt gctttcacaa aatatagcat tatacgagat aaacaacttg
     2161 ttataatttc agttaatcca gaatttaaag gactttttaa tcaattaaag aactggacaa
     2221 gatttaatct agagcaattt actaatttgg atagcacata ttcaaaaact atgtttcgat
     2281 tattaaaaca atatcgaact caaggctgga ctgaattttc caaagaaaaa ttttttgatt
     2341 tactagatat tcctaaaagt tattggaata gtccgtcaaa tattgacaaa tttatcttaa
     2401 ggccaattaa agaagaactc actcccttat ttaaaggatt aactattcgt aaaaaatatg
     2461 gtaaaggtcg aggtaaacct gttatcggtt atcgttttac ttggaaagct gaaattaacc
     2521 atgctgacga tttttccaaa gggaaacaag aagatttacg tataaaatta tttaatattg
     2581 atcacaatgg agaattaaca cgagaagaaa aatggcgcgc taaagatcga atactaaatc
     2641 ttcccttagg cactcatgaa gctgatttta aacaacaaca acaaactgaa gaaaaacagg
     2701 cagaaaaacg agaactttca aacaaactca aacaagattt actaggaaat ttacaaaact
     2761 tattcgatta agcttaagga gtatttatgg cagataaaac aatcaaagaa cttgcggaag
     2821 aattaggagt tagtaaaaca gcaattaaca aaaaagtttc tgacaaaaat cgaaaacttt
     2881 ggtttgcaaa aaacggaaac aagtttgtga ttaacgaaac cggtcaaaaa gctataaaat
     2941 caatgttttt agtcgataac gcaaacttaa atcgaaaact agttggcgaa aaatcaaaaa
     3001 ccaaaaacca taaaaacacc aaccaagttt ccgaattaaa actcctttct ttcctagaaa
     3061 aacagttaaa tcgaaaagat atacagctac aagaaaaaga taatcaaata aaacaaatgc
     3121 aaaaattact cgaccagcaa caacaactca ctctacaagc gaaccaacaa attgagaacc
     3181 tacaagaaca gctccattta acttatacag aagaaactcc aacaaccgaa tcaactgcct
     3241 tttctgaaaa aaaagcagac aatacacaac cacttattga aaaaaagtgg tggcaaattt
     3301 ggaaatctaa tagaaccaat ctctaaaatt ttctagaagt tagttcggat agtagaggaa
     3361 aaagttgtac tacctgatgg ggagatcact caaattttta tgagaaagaa aaaatagttt
     3421 acaactgtaa acaatcaatg tttatagttg taaactatca aactatatgg tgacttattg
     3481 cttaaaatag gttgaaatct cactcaattt ttggtggaaa aacaaaataa attctaatcg
     3541 attttgttga aaaagtcata aaaaaacaaa tgtgatttta acaaaatttc aaaaaaatcg
     3601 tttgaaattt cgtttatttt ttttgagaat tttgatcatc gtagggtcat tgtagaatca
     3661 ttatgagatc atcgtgggat catcgaataa agtatgggaa aggcttgcgg tgcttggtat
     3721 taagggcatt ctccgcctcc caggttcttg accattttct ctcaacgtgg taaaaatccc
     3781 acttataaga gaaaatggtc aagaacctaa acggtcggcg aagaaaacat accaagcacc
     3841 acaagcaaaa aaagttagag taacttccaa aaaagaaaaa ttttttagaa caacttttga
     3901 aaatttaatt ttgatttata tccgttcaat ttaaacattt gataaactga aggctagtat
     3961 ttaaaatgtg tatacaagtg gtagcgttaa aaaaagtgga ggtataacct atgttaaaaa
     4021 aaactgtgtt ggtactgggt tctatttctc taggaatatt aggttttagc cccatgctat
     4081 tatccgtaat tggaaaaaac aattccaacg gagaacttaa attaaattca aattattaca
     4141 acagtcctaa cgaagaattg tttgtaaaag agac
//
LOCUS       DADXTH010000126         4156 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.128, whole genome shotgun sequence.
ACCESSION   DADXTH010000126 DADXTH010000000
VERSION     DADXTH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.128"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            178..774
                     /locus_tag="IWI97_002650"
     CDS             178..774
                     /locus_tag="IWI97_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBC2464117.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IWI97_002651"
     CDS             898..2697
                     /locus_tag="IWI97_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBC2464118.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGICL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="IWI97_002652"
     CDS             complement(2975..3187)
                     /locus_tag="IWI97_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464119.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="IWI97_002653"
     CDS             3649..3798
                     /locus_tag="IWI97_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464120.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4051..>4156)
                     /locus_tag="IWI97_002654"
     CDS             complement(4051..>4156)
                     /locus_tag="IWI97_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2464121.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1229 a          706 c          728 g         1493 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctataatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc atctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata atcttaattg tcaaattaag ttagacagat catcaatact
     4081 tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaatatt
     4141 atttcgttta gatgcg
//
LOCUS       DADXTH010000127         3871 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.129, whole genome shotgun sequence.
ACCESSION   DADXTH010000127 DADXTH010000000
VERSION     DADXTH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.129"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            13..426
                     /locus_tag="IWI97_002655"
     CDS             13..426
                     /locus_tag="IWI97_002655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464122.1"
                     /translation="MEDSNHEFTDGAVTGALYTLTERVENIYKIKNNHGTYFYYSEDY
                     KDNLEITRESITTSEEYKDLTDMIKNSEVKVREILRNASQGRYTETTNLDIEHLRKLL
                     NLTDQLSNLLKTYEAEKSFEVMGNYRYSQFDNLPF"
     gene            1384..2358
                     /locus_tag="IWI97_002656"
     CDS             1384..2358
                     /locus_tag="IWI97_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBC2464123.1"
                     /translation="MANEIVKYHYELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVDDLKTTYEKILSLRFGRRSESGLSYEMFVMFTKFKIN
                     GDTPEPYVDIQIFEDALPLLNGLDSWVRYSLQQFNELQSSYSKTMFRLLKQFRTKGYA
                     YFSKEDFHELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFSFKPEIKQADDFNKHAPTKEEQNRALAQADKQKEYEQQPTTIDEKILEAKRKVELL
                     EQLKELEKKEKQDTKNQTPTEQKKSKSDSQLSEKDKQDLLNGLSNFGK"
     gene            complement(2846..3316)
                     /locus_tag="IWI97_002657"
     CDS             complement(2846..3316)
                     /locus_tag="IWI97_002657"
                     /inference="COORDINATES: protein motif:HMM:NF025324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="HBC2464124.1"
                     /translation="MADLDFYEIDPKYLKYLKKFDSSVPDYSYDKHDKFFCGVVTKMN
                     GFQYFAPISSFSRQQATNLLIKNSDGRNISSIRFCFMVPADDSVLKVKDFSLEKDQKY
                     VGLLSTELTFCRQNERRIQETAKRVYHYGIDSKHPQYRNCCKFTNLEQAATQYK"
BASE COUNT         1327 a          631 c          654 g         1259 t
ORIGIN      
        1 aaaaaaaaaa taatggaaga ttctaatcac gagtttactg atggggctgt tacaggtgct
       61 ctatatacac ttactgagag agtagaaaat atttataaga tcaaaaacaa tcatggtacg
      121 tatttttatt attctgagga ttataaagac aatttagaaa ttacccgaga gagtataact
      181 acctctgaag aatataaaga tttaaccgat atgataaaaa attctgaagt taaagtaaga
      241 gagattttac gaaatgctag tcaaggtcgg tatactgaaa cgactaattt agatatagaa
      301 catttacgta aattacttaa tttaacagat caattaagca atttattgaa aacctacgaa
      361 gcagaaaaat cttttgaggt aatgggaaat tatagatata gccaatttga taacttacct
      421 ttttagactt ttgtttgaat ttattttatt aatatctggt tttataaaaa gccctaataa
      481 tcctctctaa gagattatta gggcttttcg cataattagt agcctttatc ttcaacaggc
      541 tgttaaaacg tgaaaataag ctgaataaac ctattttaat cttaattctt tcttttatta
      601 ggttttgggt atgtagggct tgccctgcac gagcgatagc gagccttaca caaactttgt
      661 cctttcagca gacaaagtaa acgtgcgaat aaaaagcaaa tcatttttaa agctcataaa
      721 atgcgttcta aggtgtttta acgatcatgc cgacaactaa actaaataaa cactaaaacc
      781 tcttaaaacg caaaatatga cgtttaaatg gtattttagt ttgtattgct atctaaaatc
      841 tgcttttggg ttgttagggc ttgccctgac cgcctgaaag gcgcctgatg aacaagaatt
      901 acctgaaaac actaaaaccg tcttagagag ccttatatgg cgttctaaga ctaaactaat
      961 tcgcctttga acaacggttt tgattttgcc cttagcgagt gaacgaagtg aacgctgcaa
     1021 gtaaaatgtg agagcgtagc gatcactcct tttttgtctt ttggtttttt atttgcagtt
     1081 ttgcaaaatt cgcgaacctg tttatctaaa aaaatgagct tttttaggcg ggaaattttg
     1141 cccccctctt ttataatctc tttttaaacc tcttttaaac ctctttaccg tcggctgtat
     1201 cccacgcccc tcaaggggtt cagggcttta tgaaggtcat tttgtctgtt tatgaaggtc
     1261 attttgtctg tttatgaagg tcattttgtc tgtttatgaa ggtcattttg tctgtttgag
     1321 tgtcttttta taaatgactt gacttttaaa ttatgtcatt tataatcatt ttgaggtgat
     1381 tacatggcaa atgaaattgt gaagtaccat tatgaattaa atacgattcc cttacggaaa
     1441 ttcacaccag ttgaaatgaa tctgttcttc tctattgttt ctagaatgcg tgaaaaaggc
     1501 gataaaaccg ttagatttac gtttgaccaa ctgaaagatt taagtaacta taaagcgact
     1561 gctaacgttc ggtttgtaga tgatttaaaa acaacctacg agaaaatctt atctttgcgt
     1621 tttggtcgta gaagtgaatc tggactttcc tacgaaatgt ttgtaatgtt tactaaattc
     1681 aaaatcaatg gcgatactcc tgaaccctac gtagatattc aaatttttga agatgctttg
     1741 cctttgctaa atggtttaga ttcttgggta cgttattctc tccaacaatt caacgagctg
     1801 caaagcagct actctaaaac gatgttccgc ttgttaaaac aattccgaac caaaggatat
     1861 gcctattttt ccaaagaaga ttttcatgag ttgctagata ttcctaaaag ctataagcaa
     1921 cctgatatag acaagagagt tattaagcct atccgtcaag aactaaccgc aatcttcaaa
     1981 ggtttaacaa ttaaaaagaa atatggaaaa ggtcgtggta agcccgtcat tggctatcaa
     2041 ttttcgttta aaccggaaat caagcaagca gacgacttta acaaacatgc accaaccaaa
     2101 gaagaacaaa atagagcgtt agcacaagcg gataaacaaa aagaatacga gcaacaacca
     2161 acaacgattg acgagaaaat tttagaagca aaaagaaaag ttgagctact tgaacaactc
     2221 aaagagttag aaaaaaaaga aaaacaagac acaaaaaatc agacacctac agaacaaaaa
     2281 aaatcaaaaa gtgattcgca actttccgaa aaagacaaac aagatttact aaatggactt
     2341 tcaaattttg gaaaataatt tcactgattt tttcaaaaat ggggattggg gcaacgcccc
     2401 aaaaataaaa agaatcgtct gaaacgagga acaaattaaa atgcaaattt tagttgtggc
     2461 cgagtggaag atgattactt tttaccgtaa gtgtgtacac acttactaag ctcgctacaa
     2521 agaaatatct atctgcatag aaacatgagc gtagcgaatt ttaacaaaca cgcttgacgg
     2581 aaaaagagta cagtttcacg tgagtgatct gtactctttt ttagcacgat agccaccaaa
     2641 atattttggt gggtgtaagt gcgcacttag cacaacgtag aaaaaattcg gtagattttt
     2701 ttactgcgta taagtgcgcc cttgctctta ttctttgagc actggaacga cattttcaaa
     2761 tggcgttcca gtgctcattt tttcgtttcg tctcatcttt tcaaaaaaat tattgctgtt
     2821 cttgtacttt ataagctaat tcactttatt tatactgagt tgcagcttgt tctaaatttg
     2881 taaacttaca acaatttcta tactgagggt gtttagaatc aatgccataa tggtaaaccc
     2941 tttttgctgt ttcttgaatg cgacgttcat tttgtcgaca aaaggtcagt tctgtagata
     3001 ataaaccgac gtatttttga tctttttcaa gagaaaagtc tttgactttt aaaacagagt
     3061 catctgcagg aaccatgaag caaaatctaa tgctagaaat attacgtcca tctgaatttt
     3121 taattaatag attagttgct tgttgtctac taaaagacga gataggagca aaatattgga
     3181 agccattcat ttttgtaact accccacaaa aaaacttatc atgtttatca tatgaataat
     3241 ccggtacaga agaatcaaat ttttttaagt atttaaggta tttaggatct atctcataaa
     3301 aatctaaatc tgccaataat atccctccaa ttgctgtttt ttttaataac taccctatat
     3361 tttaacataa aaacaagcgc ccaagtatat acttgggcgc ttgtttcagt tttttcgaat
     3421 taggctggtt ttgatgagtt ccataaactc gaattaggct ggttttgatg agttccataa
     3481 actcgaatta ggctggtttt gatgagttcc ataaactcga attaggctgg ttttgatgag
     3541 ttccataaac tcgagagaat taataatctc tacagtaatt ttaacaaacc tttcctaata
     3601 atccaacaca agcgttgaca aaacacttat attttctaat taatcccaaa ttaattttaa
     3661 agctttataa ttaatttgat ttttattgta atagtttaaa tttaattgaa attggtatat
     3721 acttaattta tatatatttt gaagaatgga gaatgattga atgtccacgc aagcagaaaa
     3781 aatccgatta ttagctgttg atttattaaa aaacagatcc cttaacgaac ctatagataa
     3841 cgatttgtat ggtattagat acagtcagct t
//
LOCUS       DADXTH010000128         3820 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.130, whole genome shotgun sequence.
ACCESSION   DADXTH010000128 DADXTH010000000
VERSION     DADXTH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.130"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..1049
                     /locus_tag="IWI97_002658"
     CDS             33..1049
                     /locus_tag="IWI97_002658"
                     /inference="COORDINATES: protein motif:HMM:NF013539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2464125.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            1157..2026
                     /locus_tag="IWI97_002659"
     CDS             1157..2026
                     /locus_tag="IWI97_002659"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBC2464126.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            2041..2745
                     /locus_tag="IWI97_002660"
     CDS             2041..2745
                     /locus_tag="IWI97_002660"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2464127.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
BASE COUNT         1282 a          669 c          662 g         1207 t
ORIGIN      
        1 tattaccatt ggtaatcaaa ggagagattt ttatgtttaa ttttgatcaa gaaagatttt
       61 taaagactca agctggtgcc gttgcaacaa atgaacaaat ttccaatatt ttagaatcca
      121 tcgcatctga cttagaagaa atttacttta ttgggtctgg aggagttggt attctttttg
      181 attcaacaat cgaattcgcg agaactcgta gcactttccc ttttcatcgt gttatcgctg
      241 ccgaatttat gattcatccc aataaaacat tttctaacaa atctttggtt gttatcccat
      301 ctttatctgg tacaacaaaa gaaacacttg agttagtcga atactgtaat gaaatgaacg
      361 tcaaaacatt agctttggta ggaagtccta aaacgcccct agaaaaagct gcaacatatt
      421 ctatctataa cgacgtttta gatgatacat cttcagaaaa ttattacctt caaggactgc
      481 tgattgctct cttcttaatg aaacaaagaa atgaaatcga tatcaatcaa tacaatacct
      541 atattgagga tttaaaaaaa atacctgaag ccttagtgca cgctaaagag caagcggaac
      601 caatggcaaa atcttttgca aaaaaacaca aagatacttc atatcatatt ttaaccggtg
      661 ctggtgatag ctttgcggaa gcatattact atggcatgtg tattttagaa gagatgcaat
      721 ggattaagac tcgtcctgtt catgcagcag acttcttcca tggaactctt gaattaattg
      781 aagcgggtgt aagtgtcatc attacagaag gagaagatga ttctcgacct ctcacagaac
      841 gcgtgaagaa tttcgttcct aagtttacag atgttcacgt aacggttgat acaaaggact
      901 atgacttacc tggcatttcg gaagacacgc gaaaaattat ttccccagtc gtcttagcta
      961 ctgtctttga acgaattagt gcttttctag aagaagaacg taaccaccct ttaactactc
     1021 gccgttatta taagcaaatg gaatactaat cataaaaata tgaaaaaggt gaccgcgact
     1081 ccattgccca tttgattgct tcattttctc taatgggaga atagaatcgc agtcaccttt
     1141 tttaggagga tctaaaatga aaatcgcagt tatctgtagt ggtggcgact ctcccggaat
     1201 gaattcagct ttattagcaa taaaaaaaac aattgatagc gaaccagatt cggaagtagt
     1261 cttttttaag aatggttatg ccggaatatt taaggaatgt attgcctcag tttctctaaa
     1321 agaagttgag gtattcaaag ctggaacact tattggaaca gccagatttg agaaaatgag
     1381 agatctcgaa agtcggaaaa ttattattga aaacctaaat aagatggaga tcgacaactt
     1441 aattgttatt ggaggtaacg gttccttgaa aggagctgaa ctattgaaaa atgaatcaga
     1501 attacaagta tctgtcatcc cagggactat tgataacgat ttccccaata ccgattatac
     1561 acttggcttc gatagcgcct taaacactat aatccgatca atcgatgatt taacttctac
     1621 tgctaatgct catgatcgta ttattataat ggaagtcatg ggccgaggct gctttgctct
     1681 tccagagtac gcttccattg caaataatac ttattgggtt gagagagacg acaaggaata
     1741 cttacactta atatgtaaaa aatttgagtc ggagttctac tcgcctatat tgcttttacc
     1801 agaaggtata tctaacaaac aaaaaatcat tgaatttatt accaaaaatt taccaaaaga
     1861 aaccaaatac ttaaatcttg cgtatcttca gcgtggatca tctccaactt tttatgaccg
     1921 atatattggc tatttgtggg gtcaattagc ggcgaaggaa ttaattgaag gaaaatctgg
     1981 gatattaagt caaaaaaatg gtaatataat gttaatacct tggtaaaagg atggattact
     2041 atgatttttg acaaagtaaa agaacaaatt cgaaaagtgt atatcaccac tgatcggaaa
     2101 tctgaattat tgccttcgga aagagatttg gcccaggaat tttcggtgag tcgtccaaca
     2161 attcggaaag ctttagcttc tttagaagac gaaggaatta tttctaaaaa tgaaggtcgc
     2221 gggtatctag tgaaaccagc ttcagaaaaa tatgttgacc acgaactcga ctcttttatc
     2281 ggattcttcc aagatgcggt tcagcaagga aagaaaatca gttctaagat tatccaacaa
     2341 accatcatac ctgctgattc aacaattagt gaatgtttgg agattgagca gaatacttta
     2401 gtgcttgtct tggaaagaat tcgatacatc gatgactatc caacttgtat tgcaaaaagt
     2461 tgcattcccc tatctgtttt acctgaaatt gttgaacatg attttgccaa agaatccctg
     2521 ttctcagtat tagaaagtag gggaattaga ttagcttatg ccaaacgctc aattgaagtt
     2581 acaccgccaa cagatttaga aaacctttat cttgatttag aaaaaaatga gccagtcttt
     2641 gtctttacaa gtattggatt tgctaaaaat cagaaaccgt ttgaatatga aatatctaag
     2701 taccctgctt ataagacaaa gtttgaaagt actgtttcca aataattttt aaatatgctt
     2761 aaatgtataa agagcctcgc ggataacaga taaagtgtct ttccgcaagg ctctttttct
     2821 acttcctctt tatcttaatc cgctaagtag cctacagtcc aaacattatc atctaattaa
     2881 ggtgtgtgga ggtactcctc taccctaagt caaggatagt cgacatagtt gttaataagt
     2941 cttcaaagca agcctgaacc tttttcatta ggtgtgctta cttcacatat gacgctatca
     3001 aaaatgatag cacccgttga atcaaccaat tttctctaat tcatatcccc attcaaacaa
     3061 ctatatttta aaaacttttt taatctttga ttatgtgtag caaatttgtc taatttttat
     3121 ttctttgcaa aatttttcca aatttaccag ataaaagtaa ttcatattca tgattgagtc
     3181 ctttaccaat aaaacctatt tcgcaaaaat tatttgtaag cattaaggat tttctgctga
     3241 ctttcactgg aaggataacc caagaattct gaccaaagtg aaagattaag aaccatcttt
     3301 gccagtctat ctatgtgcta ttgtaactgt caacatcatg cgggcagtta ttattattta
     3361 tttgaataat atttttatta taaatcagtt ttttagattg tgcataatca ctaattgctg
     3421 tgattaatta cagaatatgg ctctctggga aaatgtgcgc gtcatgtatt caattttaaa
     3481 ttctaatttg tacaaaatga atcgtcacga ctcgatctga cgcaactcca aatgggggcg
     3541 ccgacctgta ccaagtacag tggcatgttc tttaaacatt tgaagcaaaa tctgtccatc
     3601 aaaaacctgt attttcaaag tgaacaaggt gcgctaaatc aggaaatact tacactgatc
     3661 atttcttaat ttacctactt aaccaaaatt taaatcaata tgaaagcaac acttttcaaa
     3721 ctaaagaggt ctttttaccg cttgatgttt gcttcgataa aaaagttgat gtcggattag
     3781 ttttcatttt aataaaacgt actattcctg aaaaaaattt
//
LOCUS       DADXTH010000129         3726 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.131, whole genome shotgun sequence.
ACCESSION   DADXTH010000129 DADXTH010000000
VERSION     DADXTH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.131"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1087
                     /locus_tag="IWI97_002661"
     CDS             <1..1087
                     /locus_tag="IWI97_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2464128.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IWI97_002662"
     CDS             complement(1305..2447)
                     /locus_tag="IWI97_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2464129.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IWI97_002663"
     CDS             complement(2452..2637)
                     /locus_tag="IWI97_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBC2464130.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IWI97_002664"
     CDS             complement(2991..3236)
                     /locus_tag="IWI97_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2464131.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IWI97_002665"
     CDS             complement(3230..3631)
                     /locus_tag="IWI97_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBC2464132.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1228 a          674 c          566 g         1258 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttat
//
LOCUS       DADXTH010000130         3638 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.133, whole genome shotgun sequence.
ACCESSION   DADXTH010000130 DADXTH010000000
VERSION     DADXTH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.133"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..330
                     /locus_tag="IWI97_002666"
     CDS             <1..330
                     /locus_tag="IWI97_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2464133.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..1011)
                     /locus_tag="IWI97_002667"
     CDS             complement(844..1011)
                     /locus_tag="IWI97_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBC2464134.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(1099..3024)
                     /gene="tet(S)"
                     /locus_tag="IWI97_002668"
     CDS             complement(1099..3024)
                     /gene="tet(S)"
                     /locus_tag="IWI97_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063856411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HBC2464135.1"
                     /translation="MKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSGTTKTD
                     TMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAILLISAK
                     DGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIKQTVNL
                     NLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSLYPVYH
                     GSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLYSGTLH
                     LRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGDKKRLP
                     HREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIVLSFLG
                     EVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASIGLSVT
                     PLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKYCANIL
                     NTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAFQPRRP
                     NNRIDKVRHMFNKINLH"
     gene            complement(3040..3093)
                     /locus_tag="IWI97_002669"
                     /pseudo
     CDS             complement(3040..3093)
                     /locus_tag="IWI97_002669"
                     /inference="COORDINATES: protein motif:HMM:NF019687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1169 a          606 c          563 g         1300 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcattttta aacgggtctt attgccacat atagggcaca gtatccacct
     1021 agtatttaca atatctatat cctcctgttc taattaacaa ctcaatattc attttaaaaa
     1081 tttctcttta gttcactatc aatgtaagtt gattttatta aacatatgcc ttactttgtc
     1141 tattctatta tttggacgac gtggttggaa agcagactta atgttagtaa cctgataacc
     1201 ttttaactct gttaaacaga cactgcgtcc atttgtaaag aaagttaaac tgtttcgata
     1261 ctcttgaata caacgggctg gaatttcacc aatgagaatg acctcgttac cttttaactt
     1321 agtatttaaa atatttgcac aatatttgga agcatcatta tatgctctcg aaagatattc
     1381 ttgtggtaca taaatttcga agctaagata tggctctaat aactctgtac cactctttct
     1441 aaaagcctgc tctagtacaa taggcgcaag cattcggaaa tctgctggcg tactgacagg
     1501 gctgtaatat agaccatact taaaacagat cttacagtct gttaatttcc aaccgtacaa
     1561 tccttgttca cacccatagc gtataccttc cataactgca ttttgaaatg attgatttaa
     1621 ataacctaga gaaaccaggc tctcatactg aatgccacta cccaaaggaa gtggtgttac
     1681 agataaacca atagaagccc agaaaggatt tggaggtact tcgatatcaa tggtaaattc
     1741 agattttttt aatggtcttt ccatataaat gacagttggt tttcttgttt ctatctcaat
     1801 atgatatttt tcttgaatca gagtacaagt tacctccatt tggacctcac ctaaaaaaga
     1861 tagcacaatt tcgtgagtta ctgtatctac ataatattgt agaaggggat cactatcgga
     1921 tatttcaaaa agtgcatcta gtaacttttc tctttgtact gatttacatg gttcaattgt
     1981 tgtttgtaac ataggaagag gattctcaag aatttctcta tgtggtaatc tttttttatc
     2041 tccaagtacg ttatttagtt ttaaaagctc attttttaaa ataataatct caccaggctc
     2101 tgccttatct atctggcgta attctccatt tattgaagta tacatttctg taacttttat
     2161 tttttccttt tctgatatat tgactgagtc tcgcaaatgt agcgttccac tataaagacg
     2221 tacatagaca agccgttgac catcatctga atattctact ttaaaaacat ttccacaaag
     2281 tttatctgaa ttgagttgtg tgggtgaaaa taatttgcta gtaattacct ctataagttg
     2341 tttaattcca atattattct ttgcacttcc gtgataaaca ggatataagg agcaactttg
     2401 aattctttcg ttttcctcct tttcaagttc tgcaatattc aatgttttcc caatggtata
     2461 tttttctaat aaataatcat ttcccacaat tactgtctcc cattgttctg gttcagtata
     2521 atctattaca taaggtttca aatttagatt cacagtctgc ttaattatga tgtcgtcaga
     2581 aagtttgtcc ttaatatctt gataaacatc tggcaaattt attccatttt gatcaatttt
     2641 gttaataaaa aatattatag gtatgttcat ctttctaagt gcatggaata gtatacgagt
     2701 ttgtgactgt actccatctt ttgcagagat tagcaaaata gctccatcca aaacagataa
     2761 tgaacggtat acatctgcca aaaaatccat gtgtccagga gtatctacaa tatttacttt
     2821 aacattttcc cgttgaaaag atgttattgc ggtctgaata gtaatacctc tctggcgttc
     2881 caaaaacata gtatccgttt tcgttgtacc gctatctaca cttcctaact ctttaattgc
     2941 tccgctactg tatagtaagc tttctgtcaa agtagttttt cctgcatcaa catgtgctaa
     3001 gataccgata ttaataattt tcaattttat ttcctccatt caaaagctca aaaagcagaa
     3061 aaaatcccag tgataaatac tcttatcact gggattttaa tacaaaaata gctgtatact
     3121 acacactgat agccacagat gctttatgat aaaatgataa aagtattctt aaactgggca
     3181 caaaaaacca gtccttattt taaataagga tgcttaatta attattcccg ctatctaatt
     3241 gacagtttat ttaagaatac cttgccgcat ttttattatc tccttttgaa tgttatctta
     3301 attctatcat atgatagaat gcttgtaaag ccaaccatat tttttgaacg aaagggaaat
     3361 taatgagaaa ttaatggaag ttagtgtagt agttgaagat aactcttcag aatggctatc
     3421 aaaattcagc gatttaataa cgatttacaa gttcataagg gattctttat tcatccagct
     3481 attatattac ttttcaggtc aataggacaa ctgtccacaa cctttctaca aatatattac
     3541 gtataatttt tgaaatatat actgcttaac aaaatatttt gcagtggttc caatagtttt
     3601 gaattgttaa tattaagttt gacttggaaa atatcatt
//
LOCUS       DADXTH010000131         3560 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.134, whole genome shotgun sequence.
ACCESSION   DADXTH010000131 DADXTH010000000
VERSION     DADXTH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.134"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            383..937
                     /locus_tag="IWI97_002670"
     CDS             383..937
                     /locus_tag="IWI97_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2464136.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQS
                     ITKRYIGITEDEIGASLKGFKLGI"
     gene            complement(1000..2085)
                     /gene="xerS"
                     /locus_tag="IWI97_002671"
     CDS             complement(1000..2085)
                     /gene="xerS"
                     /locus_tag="IWI97_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBC2464137.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRHLTAYDLNLYKSHLKRRAKENVKKDTTKAKLEDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDDNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKNTLSKRALIYYHRDVERDLAINALILGSGLRLSE
                     VVNINLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNSL"
     gene            2407..2640
                     /locus_tag="IWI97_002672"
     CDS             2407..2640
                     /locus_tag="IWI97_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBC2464138.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQTLSDEIDVAISQALTEYDTTFRELVDK"
     gene            2637..3044
                     /locus_tag="IWI97_002673"
     CDS             2637..3044
                     /locus_tag="IWI97_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBC2464139.1"
                     /translation="MSVHYLEAKDVIKMNVIQIKKYSPNEPIGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLVVYIATYDKDFDQLKYEVINTIKTHTKPI"
BASE COUNT         1229 a          488 c          598 g         1245 t
ORIGIN      
        1 taaaaatatt ttattaatga aaagcttggt agcacggtta ttcatatgct aatttatttc
       61 actttatagt aaggggagtt cttagtatat tgttcaaatt taaatttcat ataatttaat
      121 tatgtaaatt agctattcta agaacttcta cggataactg cccgtctaaa atttaaataa
      181 gagcttagaa tcttcatata agtaaggtta atttttttgt acaaaaaagt cagaatattc
      241 tgagttactg aaatgatata aaatcataaa ctaaacccct tgattttctt cacttttaac
      301 agagtatata tataagttca tgattatggt aaaatatata tataatcatg aactcagaat
      361 atttttaaga agggaaacaa tagtgagcta taatgtacaa ccgttaagga caaatgaaga
      421 gatacaagat tttcagtttt ggctaagaag aacaagtaat ccagaaagag atagtttttt
      481 atttttgttt ggaatcaata atgggttacg tatgtcagat attataggat taaaagttgg
      541 agacatacga ggtaaaagta agcctattat tgttgaacat aaaacaggta agcgaaaacc
      601 gatttttata gataatttga gagaagagat tttattatat actgaaggta aagaagaaaa
      661 tgattggtta tttccaagtc ggcaacaagg tcgacatata acaagggatc gtgtctatca
      721 gatatatgca gatatagcag aaaaattagg aagagatgat ataggaacac atacattaag
      781 aaaaactttt gggtatcact actacaagaa aactagagac attgctacct taatgtttat
      841 ttttaatcat agttctcaat cgattactaa aagatatatt ggaattacag aagatgaaat
      901 cggtgcttcc ttaaaaggat ttaaattagg tatttaatct ttgcatgatt agagataaaa
      961 aaaaccaact acgaaatcta tttgtagttg gacttttttt tataatgaat tcaatgcatc
     1021 aattgtagcc gcattgtcta tatgggtata taaagtcgtt gtattggtac ttgaatgtcc
     1081 caattgttgg gcagtcaata ccaaagaatt tgtctgttgg tagagtctag tagccaatgt
     1141 atgacgcagt ttatgcggac tgacttggac tctgaatcct ttcgaatatt ttgctaccat
     1201 ccgttcgatt gcaataccgg atatacgttt ggcttctcct tggtaaattg ctagaaataa
     1261 tgctttttcg ttttctgtca ctttatatct ttctttccga atagctagat aattagctaa
     1321 atattccatt gcgaacgctg ctatattgac cgcatctctc ttatttcctt ttctagttac
     1381 taccacatta ttcttatcca aagatagatc atctaagtta atatttacaa cctcggagag
     1441 tctcaatcca gatcctaaaa tcagtgcatt gatcgccagg tctctttcta catctcgatg
     1501 gtaatagatc agagctcgtt tgctcaaagt atttttatat tcattggcaa tataatctag
     1561 ataattttgt gtatcttcgt ctagaaataa ttttttctca atggcatttg cacgagctgc
     1621 taaagttgtt ttatctgtga tagttgctac ctggtccata ggattatcgt ctaagtatgg
     1681 ttttcctaga ggattattgg aacttttcga aagatattta aatagaactt tcaatgctgt
     1741 tatatttcta ttgatagtag aagtggatag gcctaagtta ttattatctt cgagcttagc
     1801 ttttgttgta tcttttttta cattttcttt tgcgcgacgt ttcaaatgag atttgtacaa
     1861 gtttaggtca taggcagtta aatgtcttat cgaggtgatt gaagtatcag gaacgataga
     1921 ctcactcaga agccattcat aaaagagctt ataatttttt agatattcta aagtggttcg
     1981 aggtgattga tctttatctt cacgatcttg aacaaaatct tttatataat cgggcatatg
     2041 aagtatgatt gtatctattt tttgatatat ttctgtattt ttcatgtcat tcatcctttc
     2101 aataagtagg agagaggagt acccctacaa ttggactata ccaattatag ggacgtttgt
     2161 caattggtat gctatatttt ttttggaaaa cacaaattat ataaggttct atataaccat
     2221 ataaaataaa tggtatatat aaataggcga ggggatagca gtttgtattc taaaaatttt
     2281 tttggttttt cttaattcta tagaaagaac aatctttcta tagagttaga catgagaggg
     2341 actacgattg atttaaaatc aataagcgta tatactgtat atacaaacaa aaaaggggag
     2401 aagactatgg caatcaaaga aagaaaattc agagaagtag ggaattcagt agtattaact
     2461 atttcaaagg agtttcttaa agaaaatggg ttaaatcctg gagatacggt ttttttagat
     2521 gaagaaaaac ttcgagcagc aattacaaaa aaacaaacct tatctgatga gatcgatgta
     2581 gcaatatctc aagcattaac tgaatatgat actactttta gagagcttgt cgataaatga
     2641 gtgttcatta cctagaagcc aaagatgtta taaaaatgaa tgtgatccaa ataaaaaaat
     2701 actctcctaa tgaacccatt ggtgtaaagg acgcgaatgc tttagaaatg tgtgtaggac
     2761 aacttcgagc ctcagctttt ggtgaagagg tttatccttc gatttatgaa aaagcagcta
     2821 tcctacttat acaattgata aagaaacatc cttttcataa tgcaaataaa cgtactgctt
     2881 tcttagctac ttttgtcttt ttgaaaatca atggacagct tttagctatt gatcaaaaag
     2941 atgctgtaaa tttagtggta tatattgcta catatgataa ggattttgac caactaaaat
     3001 atgaagtgat caacactata aagacacata cgaaacccat ttaaggagtt gctgtatgtg
     3061 tctttatagt gttattagct gctatagcga aataattgaa gtaaattaca aatagctata
     3121 attcaaatcc aagtatatag aaaacgtaaa ttttctatat acttggatat gctggaatgt
     3181 ttaagagagt aattacattt ctagaaaata caataaaaat tacaggataa ttagccatat
     3241 cctacctcct ctagcagttg ctcaaaagtt agcactaagt aacttctttt ttgttgctgg
     3301 taacaattta tcaatactaa aatagctttt aagtatctaa taaatactaa ttttaaagct
     3361 cttgattatt atgaaacacg atataacaat attgctatat cgtgctattg taatattata
     3421 catttgaaga cagcgtattc ttgttctaat atcaggaatt ttttataaat atcagtcata
     3481 ttaagactca aaatttattg gtatgttcct ctgtatagag tttgaccgtc taccattata
     3541 tagcaattat tatcattgtt
//
LOCUS       DADXTH010000132         3553 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.135, whole genome shotgun sequence.
ACCESSION   DADXTH010000132 DADXTH010000000
VERSION     DADXTH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.135"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..387)
                     /locus_tag="IWI97_002674"
     CDS             complement(37..387)
                     /locus_tag="IWI97_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464140.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTNHNLAKEQMTTE
                     DIVKVLNAAVIKNRSVCIQKEERNAEGYYPYDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(380..1705)
                     /locus_tag="IWI97_002675"
     CDS             complement(380..1705)
                     /locus_tag="IWI97_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBC2464141.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVTDEDLLIYSIDESILKVTPSLNLFTTEGTRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLFYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(1856..3046)
                     /locus_tag="IWI97_002676"
                     /pseudo
     CDS             complement(1856..3046)
                     /locus_tag="IWI97_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1033 a          682 c          570 g         1268 t
ORIGIN      
        1 atgttatata ctttagctca tattgctgga taatgttcat tctagtcgac tccacttatt
       61 atcctggtaa aattcaatgt gccgtatttc gtcgtagtga attttgtctt ttccaacgta
      121 gattcccagc tcatcactac ctaagatctt tccaacaata tcgtatggat agtatccttc
      181 agcgtttcgt tcttcttttt ggatacatac tgatctattt tttatgactg cagcgttcaa
      241 tactttcaca atatcttcag tcgtcatttg ttcttttgcc aaattatgat tggtacgttg
      301 ctcattgtcc gcatcgatct cttcagtatg atcagataaa taaaaccctg cccactttaa
      361 acgaccacga tcattgtaat caatcatttt taattccccc atttccgccc gcatgaccac
      421 caacaagtgt gcttctagta atgcttctag cgccttctaa cattgaagaa gcatgtacaa
      481 tcgacgtaaa accatatttt tgccgaatct tgtcaataat tttatctaaa ttttcttcgt
      541 caatttgctt ttgtggatca ctaaataaat ccagctgaag cgaatcagta taaaatagct
      601 tgctgtaagt gattccgacg tggcggactt cttgtccttc ataatactta tcaaacaaga
      661 aaagacaata attctgcaat tcctttgtat tgtctgtgat agggattttc atctggtgtg
      721 agaaccctgg tctcgtttct aagatcgatg taccaatgtt taaatggaca catcctgttt
      781 tacaattatg ctgtctgata cgaatggcta cttgttccgc catttctttt acaaccagct
      841 cgatttcatt tcttctagta taatcacgtg gcaacacttg tgaattacca taggactttt
      901 cttttgtcgg ttctggtggg attgctatat cagttcgatc aataccattt gcatgaaagt
      961 atagctgtaa gccaatcaca cctaaacggc tcttaatggt gtatggattc cagttagcaa
     1021 gatctctaat gctcataatt cccatctgat tgagtctttt cttcattcga gagccaatgc
     1081 cccaaaagtc cgtcatttca ggaatattcc atactttttc aggaacgttt tcgtacgtcc
     1141 attccgcaat aaatccttca ttatgctttg cttcattgtc taaggctagt ttagccaaaa
     1201 ggggattatc gccgacgcct accgtagcaa tcaaacctaa ctcttcttta atacgttctt
     1261 ggatcatttg agcgagcttc ttccttcgct gacttcgtgt tccttcagtc gtaaaaagat
     1321 tcagtgatgg ggtcactttt aagattgatt catcgatcga gtaaatcagt agatcttcat
     1381 cagtcacata tcttctgaaa atattattta cctgcatatt tcgcttgata tataggttca
     1441 tacgtggtgg aacaacgtgt aatgttttag gaaatggttg tggtaagtca cgtggtctac
     1501 tcacatttgt aataccatac cgcttttttg cttcaggaga agaagctaat atcaatcctg
     1561 aaccagtatt atcagatcga ctcataacaa caagttctgt tgttaatgga tctaaatttc
     1621 tttctataca ttcgacactt gcgtaaaagc ttttcatatc gattaaaaag taatcattta
     1681 ctggctcttt tgaataatcc atcataaata acactccatt ctttcacatt atacaaacat
     1741 atgttcgata ttgtaaagaa gaaatagcta aatacataaa aaaagaagca taggtatgct
     1801 atccccctgg tagcgtcaag aattatgtgt aaaagtatac ccttggtaaa gcagattagc
     1861 tcttgaacca atcttctctg gttgtttgtc caaaacctct atgtacgtga ttcatgtttt
     1921 tctcgttgta acgattgaac tgaatcacaa ggtatttctc ttcagatttt tccgttggaa
     1981 attgttattt ctttttgaaa cttcgtttta attgtttatt gtttccttca atcagatttg
     2041 ttgaataaat cgtatgccta acactttctg gataatcata gaatgtaaac agatagtcat
     2101 tgttgctgag acttttcacc attgatggat atttgtgttt ccacctatcg atgaatttct
     2161 ttagagaagt taaagcttct tcttttgatt tggaccgata tacctcttta aattcattgg
     2221 ccacttcttt tctgtcactg actcttactt ttgcacatat atttctttga atatggacca
     2281 ggcatctttg aattttagag cccgggaaaa tgtcctgtat tacattttcc atcccagaca
     2341 atccgtctgt acaaaataaa gaaatacgtt caacccctct tgatttaagg tctttcagaa
     2401 gttcttccca tatctcagcg gactcgtttg gtgcaatcat atagccaaga atttcttttg
     2461 taccttctac tgtgattcct agtgctatat ggaccatttc cctggataca gtatcacggc
     2521 gtaaaggcat ggatgttcca tcaagatata caacggcata ctctttgttc aatattctgt
     2581 ttttgaagga atctacacat tccagagctt tgtccgtaat attagatatg gtctgtttag
     2641 aatagcgagc tccacataat ttttggatca tggaacaaat ttctgaatgg ctcattcctt
     2701 cttcaaataa atccaaaatc gttgtttcgg taaatagatc tcgtcttctg tatttaggca
     2761 gaagagaagt gaaaaattct cccattctat ctcttggaat attgagtgta atagttccat
     2821 attttgtatc aaactttctt tgataggaac catttctaga attttcgtta ttggttctag
     2881 cgtatggttc ataatcaaga aaagccgtca actcatatgc gagtatttca ttgagagatt
     2941 tctccaactc tgaacgaaac agttcggtta cagcctgttc aaaagctgga agattgttgt
     3001 cattagaaaa taggtttttc attaaagatg tagtaaaata gttcatgggt atattccttt
     3061 cgtaaattgt ttttggtcga actaatttta ctaaggatat accttttttt ctacatttct
     3121 agaaaaacga atttacacaa gatattttac actctctatt tttctatcaa aaaatccaga
     3181 aataactaaa tataggtatt tctggattat agtataatat ttacgccaca gcccccaatg
     3241 attctttttg gaatcatgat caaggacgga catacacaaa aatattttgc ccattcgatc
     3301 cgaatagatt aacgactaat ctagtaagta atatagattg gtacgtagtc aagctgcaca
     3361 taatttagat caattttagt tcttttttat atttctatga tctgctcatt tacttttata
     3421 tgatggctta cagtaacata aataaatatg ataatcgctg gtagtttatc caagagtact
     3481 caataaaata caaaatggat tgttcaagcg cctatcataa tgataaaatg aaacaaataa
     3541 acgatacaag gag
//
LOCUS       DADXTH010000133         3517 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.136, whole genome shotgun sequence.
ACCESSION   DADXTH010000133 DADXTH010000000
VERSION     DADXTH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.136"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            128..340
                     /locus_tag="IWI97_002677"
     CDS             128..340
                     /locus_tag="IWI97_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464142.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            complement(564..1523)
                     /locus_tag="IWI97_002678"
     CDS             complement(564..1523)
                     /locus_tag="IWI97_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HBC2464143.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            1617..2087
                     /locus_tag="IWI97_002679"
                     /pseudo
     CDS             1617..2087
                     /locus_tag="IWI97_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            2094..2606
                     /locus_tag="IWI97_002680"
     CDS             2094..2606
                     /locus_tag="IWI97_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464144.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            2877..3500
                     /locus_tag="IWI97_002681"
     CDS             2877..3500
                     /locus_tag="IWI97_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2464145.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT         1203 a          522 c          626 g         1166 t
ORIGIN      
        1 cactatatat tccgaaaagt gagtatctcc attcataaaa taatcaacat atttaaaaaa
       61 tctaattagc ttttttagtc aatctaaagt tctttattct atgatataat gttactgtag
      121 aaagtttgtg gcggtttggc aaatccaatt gaaggagcgt ggtgcctatg ggcttattag
      181 cttatcggac gtactctgta gaaaggaaat gcctgatttg ttctcattgg atcaaatcat
      241 tgctattatc ggagcagcag cagtagttat tactgccgta gcgaaagcta gcgattcgat
      301 tttagcttgg ttaaagttca aaaaagaaca aaaaaaatag agccgtctca acttttggcg
      361 aggtagacga gctctaaagc tttatttaca tgccatccgc tttgaacgga ctacactggg
      421 catgtgacag catgcccttt catttatatt atatctcatt cttttatata atgaaagtaa
      481 ataaatcatg gaggataaaa tatggataaa tttaatagat taacattaat aaatcagttt
      541 gaaattttaa aagctttgat ttgtcaaatt aagctagaca aaatatcttc attcatgtaa
      601 ctcataaata cttccagggg tgtttgataa tccaaggact ttcgtggaat attgtttctc
      661 ttattcgcca ctgaagagat aaaagcttgg tcgacttgat taaaatccat gttttttggc
      721 agaccatctt ttcgcagcaa gccattggaa ttttcattca gtgctcgttg cgaaggagtc
      781 cctggatcag cgaaataaat agcgatgtca tgttggttac ttaaagcctt ccaattagag
      841 aattctttcc cacaatcaaa ggtaatggat ttaaataaat gtctgggaat cgatccaaac
      901 cagtggttca gagtattctc aatatcgatc gccttgcgcc cctcgggctt caaggtgatg
      961 atgattttcg atagacgttc taccaaagtt atgacagcac tcttatgatg aatgcctaca
     1021 atggtatcgc cttcgatatg gccgaactct tctttaaatg tcgggtaatc agtttctctt
     1081 tcaacgatat tccgtttaaa agcctgtttt cctcggcgtt cttgatgtcc atttggtttc
     1141 cgcttaccct tcatgggcaa agtcgattca tcgaacacct tttccttgaa ctggcgataa
     1201 agggttctta cggaacagct gattggtttc tctttacgcc caatgataac gtctggcgtc
     1261 cagccttggg caattttttc tttaatgtag gactgttgtt tctttggcaa gacgatgttt
     1321 cgtctgccac accgtttctt attttcttta tactgttggt agtattcgag ggccgtatgg
     1381 ccttctttca agaaattaat gacgttataa atcggtgttc gagatcgttt caggtactca
     1441 gcaattttca caattttgat gttttggtgg tagtaagact ctatcatcac cagttcatcc
     1501 gtggtaagat gggtgtaggt cattcgtgat cactcctatg ttttctttag tcggaattac
     1561 attttgagtg tagcacgaat ggcttattta tttgtctagc ttaattttac aatcggcgag
     1621 ctttaaatcc taacgaagaa aaatactatg ctgaaaaaat agaaatacta acagaaggtt
     1681 acgaatatca ctatagtgaa atatttgaaa atatttctga tccattgcca gaagaagatt
     1741 cgcggtttgt tctagacata ttaaatatgt atagagatat tacttttagt aaaaataaat
     1801 taaaagatat aaattcaatt gataattatt atatacaatt taaaggtttt gactttaata
     1861 gctctaccga atttaagtta gcactttatg cacaattttt tattgaaaaa ttaaataggt
     1921 ttcaagaaat agttgaagat agtgatttta acacttttaa tagccataaa ccgatgagag
     1981 gtacttatat aaaatttctc gaaaattata atgatttaaa atctaccaat aattatcaat
     2041 ttggaaactt gtcttatgag atgataagta aagttctaag tttgtgatga taaatgaaat
     2101 taaatagtat aaatgattta tttgctatta taggtgttat tgctaacata cttttttcgt
     2161 gtttgaattt tagatttact caaaagaaca aaaagaacaa actcagtata gaacaaatga
     2221 aaaaagacat tgaaactgat ttaaaaaatg accataaaga aagaagaaaa attgaagaat
     2281 atacttcttt aatggaaaag tgtaaagcaa ctgatgcaat ttttagcttt ttaagaaaaa
     2341 gtgatgagat aatcacacgt gaaaatatag atttagaaaa tgagattagt aaacttgaat
     2401 ctatcttttc cgatataaat tctcttaagt atttaatcgt cgaagaaaat tcggatagct
     2461 ttttagaatt tgaaaaatat ctaacagatc ttatagataa tagaaataat gaattaatga
     2521 gacaaaacat tgcaagattc cttactaaat tagtttctga atggaaaagt attatttctc
     2581 aaaatatgga taaattaaga tattaaggat gttagtttta aaataaacta atgtccttat
     2641 ttttgtgttc acatttctgt tacctaagaa atacttaaag gaggtgatgt agccagcaac
     2701 gactacaact tgaacccgct atctacagtt tatttgttgt ttcttttggg tatactctaa
     2761 tggtacaaat ccatatttgc ttaaagcgtt gatttttcag cggtttttct ttattgcaaa
     2821 gtgtacccta agacctgtat actaatagta aatagattgg ataggaatga tggcaaatgt
     2881 atttagctta tttaagagtt tcttccgacg atcaaagttt agctcggcaa cataagctaa
     2941 ttgaaaattg gtcagtcaaa aataatattg ctgaagacga tttaaaaatt ttcgaggaaa
     3001 aagtctctgg aaaaaatatt gaaaacagac cagagctgca agagcttatt tctttcatac
     3061 gtgaacgaga tacgattatt attcctagtc ttgatcgatt agcaagaaat agtaaagata
     3121 caaaagactt acttcaattg tttcgtagta aaggtgcttc tattgaaata ttagacctac
     3181 cgtcgtttaa tggggtaact gaccctgcat taagagactt gctaacaaat ttagtcatag
     3241 aagtgtttag ctatgttgct gaaaacgaac ggaaaaaaat caaagagcgt caaaaacaag
     3301 gcattgaaat tgcaaagaaa aatggtcgtt atactggtaa aaagattcaa taccatgcca
     3361 atgcaacagg aaaaaacaaa ttgatttacg atgaaattgt tgatggtctg aagaaaaaag
     3421 aaactatttc taaaatagca aagaacgcag gagttacaag aaaaaccgtt tatcgagtaa
     3481 aaagagattt aggaatgtaa tatgttccag ttagaaa
//
LOCUS       DADXTH010000134         3499 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.137, whole genome shotgun sequence.
ACCESSION   DADXTH010000134 DADXTH010000000
VERSION     DADXTH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.137"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IWI97_002682"
     CDS             <1..379
                     /locus_tag="IWI97_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBC2464146.1"
                     /translation="KELKITSFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGT
                     ILVIAMLITPAATAYLLTNHLLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLAC
                     TAFFILAFLFSPTKGILFQKGSR"
     gene            380..1330
                     /locus_tag="IWI97_002683"
     CDS             380..1330
                     /locus_tag="IWI97_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBC2464147.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            1471..2223
                     /locus_tag="IWI97_002684"
     CDS             1471..2223
                     /locus_tag="IWI97_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464148.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            2242..3393
                     /gene="queG"
                     /locus_tag="IWI97_002685"
     CDS             2242..3393
                     /gene="queG"
                     /locus_tag="IWI97_002685"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBC2464149.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         1231 a          574 c          753 g          941 t
ORIGIN      
        1 taaagaattg aagatcacat cattcgatcc gacaatggca aaagcttatg ggctgaacac
       61 atcgctgatc cattatcttt tgatgttctt tttgacatta gtggctgtag tcagtttaca
      121 gacagtagga acaatcttgg tgattgccat gttgatcaca ccagccgcca cggcttactt
      181 gctaacgaac catttactga aaatgatcat tacagctgca ggaatcggta tgctaagtgc
      241 agttgtcggt gtgtttttca gttatagtta caattggcca tcaggagcta cgatcgtgtt
      301 agcatgtacc gcatttttta tccttgcttt tttattttct ccaacaaaag gaattttatt
      361 tcagaaagga agcagataga tgaaaacaaa aaaatcactc tttttaatct tggcagttag
      421 tttcttggta ttagccggtt gcggaaagca ggcttctgac caagcggatg aaggaagtaa
      481 agaaaagttg tcagtcgtgg ctaccaattc gatcttggcg gacatggcaa aagaggtcgg
      541 aacagatcaa atagatatcc acagtatcgt accagtcgga acagatccgc atgaatatga
      601 agtactacca gaagacatca aaaaggcaag cgatgcagat gttattttat acaacggttt
      661 gaatcttgaa acaggtaaca gctggttcga taacttgatg gaaacggcta aaaaagaaga
      721 aggaaaagat tattttgcag ttagcaaaaa tgtagaacct ctttatttaa ctagcggtga
      781 agaacataca aaagcagatc cccatgcttg gctagaccta tctaacggaa taaaatatgt
      841 ggaggaaatc gcacgtatat tctctgaaaa agatgcagaa aatgcgacac tctataaaaa
      901 aaatgcagaa gcatatgtgg aaaaactaaa agaattagat acacaagcga aggaaagttt
      961 tgcttctatc gaagagaaca aaaaattatt agtaacaagt gaaggggcat tcaagtattt
     1021 ttcacgagca tatgatttgc cagcagctta tatatgggag atcaatacag aaagtcaagg
     1081 tacgcctgat caaatgaaag cgatcattga tcagataaga gcaaaagaag taccggtttt
     1141 attcgtggaa accagtgtag actcaagaag catggaacgg gtagcaaaag aaacgggttt
     1201 gaaaatctat gataaactgt tcactgattc catagcaaaa gaaggagaac aaggagattc
     1261 ttactatcag atgatgaaat ggaatattga aacgatccat gaaggtttgt cacaaacaaa
     1321 agaaagctga aaagtgcaag gtctagcttg aggagcagac cttgcatttt ttataagaga
     1381 tatgaaaaaa aattatgctg tttttcgtcg tacagatacc ttttttgaag aaattccgtt
     1441 atagttagag taaggaagga aggtgtggct atgaatttta cacgtaaaga taaatatacg
     1501 attaaagaag aagtctttcg attgatcagc cagcaggaaa ataccgacaa ggatcaagac
     1561 cttcaatttc ttgaagcaga attagctaaa ttattcatag caaatcaaaa tgagacaaca
     1621 gaaccagatg aagagctgta cataggcaaa tggttgtttg atgaatttca gcctttagat
     1681 gaaaataccg gtatcggtta ttttgtgttc ctttctccag tcggcttcac acctggcgta
     1741 ttagttaaac aaaacgacga aataaccgtc aaagcatggc ctgcattatt ttcagtcatc
     1801 aaagcagaag gagtaatcaa aaagatccag tcggcctatt ataaaagtca atttattgcc
     1861 aacagcaaat taatgaatat gttttataat cgtctagtag aaattgctag gcctgggaaa
     1921 atcccgcaaa cattcacgta cacacagcaa aaaaatctct atgaagaagt attgtattcc
     1981 cgcgatctgt ctgagttttc gccatgggaa gaggtaaggc tccatgaact gttaagtaca
     2041 aaaccagttt cattgaattt ttttaatgca ttagataaca aaagaaaatt aatttcaaca
     2101 aattattggg aagtatttgc tgcaagaaaa aaatggatgg aagaaaagaa gattgccgat
     2161 cagcagatcc agtttgtcca gctgaaaaaa gaaaaactat attatgggtt atacagccgt
     2221 taacagcaag cgaggaagac catgacatta aaagaagaaa tcatacaaga aagcaaacga
     2281 ttaggaattg acaaaatcgg gttcaccacg gcggaacctt ttgattcatt aaaagaatcg
     2341 ttggaagaac aaaaagcagc tggccatact tctggctttg aacatccaaa tatcgatgag
     2401 cgtctttatc cagaaatgac atttgatcag ccgaaatcga tcatttccat tgcacttgcg
     2461 tatcctacta ggatccatga agaagtgcct agggatgaaa aacgcgggca gtttgctcgt
     2521 gcttcttggg ggatcgatta ccatgatata ctcagagacc gtttgaaccg attgatcgaa
     2581 ttcatccaaa caagagcaga aaaatggcaa aaagaagagg aatggcgtct agctccgcaa
     2641 gtagatactg gagaactgat cgatgtcgct gtagcacagc gagctggttt aggatttatt
     2701 ggacgaaacg gattgttgat cactgaagag ttcggttcat ttgtctatct tggtgagatc
     2761 gttactaata ttgtatttga gcctgataaa ccaggagaat ttggttgcgg tgactgtatg
     2821 cgctgcgtga ctgcttgtcc gacaaaagca ttgcttggtg acggaagaat gaatgcccag
     2881 cgatgtttgt cttatcaaac tcagacaaaa gggatgatgc cggaagaata tcgtaaaaaa
     2941 atgcgaaatg tcatttatgg atgtgatatc tgccagttag tctgccctta caatcaagga
     3001 aaagattttc attttcatga agagatggaa ccaaaagtag acgaaatata tccaaaatta
     3061 aagccaatgc tttcgatgtc taataaagac ttcaaacagc aatttgggca tttagctggt
     3121 tcatggcgtg ggaagaaacc tttgcagaga aacgcattga ttgctttagc taatctaggt
     3181 gatcgaacgg cattgcctga gatttcagaa tgtttaacag atgtccgccc tgtcatcaga
     3241 ggaacagcag cttgggctat tggacgtcta ggaacaaaag aaccggaaaa atggcttgat
     3301 gtattagatg aaatattagc taaagaaaca gaagaagtcg ttataaacga attggaacat
     3361 gcccagaaat tattgcggag aaagttaaaa taaaaaaata gttaacatta ttcaaagttt
     3421 ttgtccagta attgttgtaa caattactgg tttttttgta ttttttacat ttttttaaga
     3481 gaatttgata aaaatcaaa
//
LOCUS       DADXTH010000135         3062 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.138, whole genome shotgun sequence.
ACCESSION   DADXTH010000135 DADXTH010000000
VERSION     DADXTH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.138"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(416..1165)
                     /locus_tag="IWI97_002686"
     CDS             complement(416..1165)
                     /locus_tag="IWI97_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBC2464150.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1351..2055
                     /locus_tag="IWI97_002687"
     CDS             1351..2055
                     /locus_tag="IWI97_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBC2464151.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2095..2652
                     /locus_tag="IWI97_002688"
     CDS             2095..2652
                     /locus_tag="IWI97_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2464152.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2660..>3062
                     /locus_tag="IWI97_002689"
     CDS             2660..>3062
                     /locus_tag="IWI97_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBC2464153.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEI"
BASE COUNT         1021 a          448 c          612 g          981 t
ORIGIN      
        1 aaaaaatatc accataattg gtttaggctt aaccgtatat ttatttacag aatttgttta
       61 tagacatgtg aatttatttg cagaaccttt atttgtatta tacgaatcaa attcctcaat
      121 cgtcagtgaa cttgctaatt taatgatagc attgctgttt actcccgcgt tgagagtcat
      181 aactattttg gtctgtttag tgctattgtt ttttgttaat aaaaagaaat aacgtagtat
      241 ttcaaaagac aaagaagtgg gatgatgatt gctatagtat gttatacgtg gtgctaggta
      301 agtgagaata aacaaaaaag gaaacacgca ataaagttta tttcaaaact ttattgcgtg
      361 tttctttttt agatttttta tatctgttgc tttagtagtt accaatagtt ttcgcttaat
      421 caatcaaaac gattttttgt ttattgtatt ctttacgaac cttttcagga atttcttctg
      481 aaataagata ggtaaggtct tcaattggac cgacgatatg attactgatg gtgccgaatt
      541 tatcattagt tatcatccca agtacttcag tagaaatacg tatcatctca cgtttaatgt
      601 cggattcttg catagttggc acgctcgttc caacatctag atctatgtta taaataccca
      661 taatataaag gtcggctcga atgttttgta gttgcttata agcttgtacc ccaatattga
      721 tcatagattg tttataaaaa gtaccgccaa tattgataat ttccaaatta ggatgctgtg
      781 ctaatgctat ggcaatcggc gggctattgg taatgattgt tgcacgaaaa tcttgtggga
      841 tttcttttgc taaagccaag ttagttgtac ttccatcaat gatgatgaca gattcttctt
      901 ttaaaaaagg gagggcttta tgagctaatg cagttttttg ttcgctagct acagtttctc
      961 ggtagttaaa attggtaatt ggcggaccaa cacgtaaagc gccgctgtgt actcttttta
     1021 agagaccttg gctatccaaa tctttcaaat ctcgtcgaat cgtgtcttcg gatacattaa
     1081 aacgttgact taaatcacta gcgataactt tttgttctgc gtttaataaa tctagaattt
     1141 tttgatgacg ttctaatttc aacatgttgc gtgctccttc ttatttattc ttgtttatca
     1201 tgtcatttat gcgtttttat gtcaataatt tccgttttta atacactaaa aaggcataaa
     1261 caagcaaaaa caaaatattt ttatgcagtt ttatgttgac tagtatttgt taagcggtta
     1321 caatgagggg gtaattaaag gagggaatgt atggagaaaa aaattatttt gtcgccttca
     1381 cttatgtgtg gggatctagc aaatttggaa ggaagcatcc aagaactaga aagtattggg
     1441 atggattcct tgcatattga cgtgattgac ggttctttta gtcccagtat gcctttaggg
     1501 atcgaaacaa tcaaaagaat tagagaaatc acttcaatga attttgatgt acacatcatg
     1561 gctaaggata acgataacga attttttatt ggagaaatgt tgaaaattgg cgtagagagc
     1621 attacttttc attatgaaac aagcctccat attgatcgat atatccagct catcaagaat
     1681 tctggaacaa aagtagggat agctttgaat ccagcaactc cgttaaatgt attacaatat
     1741 attttacccg agattgatag gatttgtttg atgctgatta atccaggttt tgcaacaaac
     1801 aagtcagaaa aacaagtttc ttacgcgcta aaaaaaatta cagatctttg ccagttagta
     1861 aatgagaaag agttagcagt agatatccaa gtggatggac gggtatcgtt agaaacgatc
     1921 ccgggattat tagatgcagg cgcaacaaat ctggtattag gaagtacaag tttgtttctt
     1981 aaaggaaata cattagcaga aaatcaaaaa ctactttatc aagcgatgaa gcaatcagtt
     2041 aggaaaaatc aatagatgta gtggtcctga ataatagaaa tggaggggaa atagttgagt
     2101 tataaggcgg cgaaacaaaa tattgtaaga gaaattactg aaacattaga ccgtatttct
     2161 tcaaaagatg tggaacaatt gataaagaaa attgaaaaag cggaacgagt ttttttcgtg
     2221 ggggttggca gagtactgtt agcgctagaa ggaatggcaa aacgcttagg gcatctagga
     2281 attcagacag tagtcgtagg acaaatcact gaaccggcaa ttacagataa agatttattg
     2341 attgttggtt ctggtagtgg ggaaagtgct ttcccactaa ctattgcaaa aaaagcaaag
     2401 gaatttgggt cccaagttgc ccatatagga tcaaacccag acagcacaat gaaagaatat
     2461 tcagatcttt ttattcgtat acccgtacaa acaaaattag cgttaccaga ggaaatttct
     2521 tctatacagc caatgactag cctgtttgaa caaagtgttc ttttgttagg agatagtatt
     2581 gctttagtga tgatagaaga aaaagcaatt gatatgtcaa agttgtggca ataccatgct
     2641 aacttagagt aggtgaaaaa tgaaagcggt acattttgga gcagggaaca tcggcagagg
     2701 gcttattgga caaattctac atcagaatgg attcgatcta tgttttattg atacgaatcc
     2761 tgaactgatc catcaattaa atcaagataa gggttatact atcgaaatta ttgatgaaat
     2821 gaacaggagt gatttcattg atcatgttgt ggcactgaat agtttatccg agctagaaga
     2881 ggtgattaca gcaacaaaaa atgcagatat cttaacaaca tctgttggcg caataaattt
     2941 agtaaaaatt gcaccgatca ttgcgcaagc cttaagtaag cgatttgaac aaaagcatag
     3001 catcaatatc gtagctaatg aaaatgtgat aaatgcaagt gatgtgttga aaaaagaaat
     3061 tt
//
LOCUS       DADXTH010000136         3028 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.140, whole genome shotgun sequence.
ACCESSION   DADXTH010000136 DADXTH010000000
VERSION     DADXTH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.140"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            23..694
                     /locus_tag="IWI97_002690"
     CDS             23..694
                     /locus_tag="IWI97_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2464154.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="IWI97_002691"
     CDS             753..1193
                     /locus_tag="IWI97_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464155.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="IWI97_002692"
     CDS             1205..1873
                     /locus_tag="IWI97_002692"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBC2464156.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="IWI97_002693"
     CDS             1937..2560
                     /locus_tag="IWI97_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBC2464157.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="IWI97_002694"
     CDS             2616..3002
                     /locus_tag="IWI97_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBC2464158.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
BASE COUNT         1085 a          491 c          631 g          821 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagat
//
LOCUS       DADXTH010000137         3007 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.141, whole genome shotgun sequence.
ACCESSION   DADXTH010000137 DADXTH010000000
VERSION     DADXTH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.141"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(105..1610)
                     /locus_tag="IWI97_002695"
     CDS             complement(105..1610)
                     /locus_tag="IWI97_002695"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBC2464159.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1702..2292)
                     /locus_tag="IWI97_002696"
     CDS             complement(1702..2292)
                     /locus_tag="IWI97_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2464160.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2471..>3007
                     /locus_tag="IWI97_002697"
     CDS             2471..>3007
                     /locus_tag="IWI97_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBC2464161.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVAR"
BASE COUNT          916 a          572 c          580 g          939 t
ORIGIN      
        1 gctagtgaaa agttaaaatg atttctaaga aaaaaggaac tgtgacaaaa aatcacagtc
       61 cctttttttc ttagaaatgg gaaaccacta gtatataaat accattattc ttcaatcaac
      121 cgcttcacga ttgcataatc cccttcataa ggttcatcta caccattgat tttttgatag
      181 ggatccacgc cttttcccgc aataacgact gaatcttgcg gagcgctttc tgctaaagcg
      241 cgtcgaatcg cttcaggacg atccatttca taatgaacga tcaaatctgg attagtaatt
      301 gcttcattga tttcttccgc gatttttttc ggatcttcaa atgctgggtc atctgccgtc
      361 aaaaaggcga tgtcagctgt ttctgatagc acttttccaa aatcatgtcg acgggagatt
      421 gctttgtttc ctgggctgcc taatacaaca attacacggc catctggatg ttcatttttg
      481 gcaaatttca gcaatgtttt caagcttaga taattatgtg catagtccac atagacatgc
      541 gctccattgg attggatcaa ttgatccatt cggcctggta cacgagcttc ttttaatccg
      601 tcttgtgcat cttgactgga ggcacctgtt agtgctgcgg caatgatcgc actggctgca
      661 ttttcttggt tgaatcctcc agccagcaaa atatcataag cagtatcgca gatagctaag
      721 cgatcttcat gagaagataa agtaaaaccg tgagtacctt tatctgaacg gatcacttga
      781 taattgcttt cttccgaacg accataagtg atcgttggaa tctggaatag atcggctgtt
      841 tccctaagta aatcaaaata gtcactttca tgatttaaaa caactgtttt tgaattcaag
      901 atcaattgtc gcttgcaata gaaatagtca tcaaatgtcg gatgttcgat cggactgata
      961 tggtcaggcg agatattcaa gaagattccg acatctaaag tcaagccgta tacgcgttgt
     1021 gttttgtaag cttgtgaaga aacttccatc actagatgag tcataccatt ttcaactgct
     1081 tctgacatca tacgatataa atctaatgac tcaggcgttg tcaaatggga tttgaaatat
     1141 gttttgccat ctaacgttgt attcatagta gaaagcaatg ctgttttttt atttgttgtg
     1201 tgatccaaga ttgcttttgt aaagtacgca gcagttgttt taccttttgt tccagtgaat
     1261 ccgatcactt tcaatttatt ttgcggatag tcgtaaaaag ccatagcaag aagcgccatt
     1321 gctttacgga tatccgttac gatgattcct actgctgaat tttcgtatgg ttgttctgat
     1381 acataatatt gaataccctg ttcgattgct gaatccaaat attcttcttt gaaattcatt
     1441 cctttacaga aaaacaaagt agaagcatca gcttttcttg aatcataact taactgaaga
     1501 aattcagctt tttctggtac atcaagatgc cagcctcttg ttgaaacaaa ttcttttaat
     1561 agattttctt ttaatagtaa atgacggatg tcttctaaag agaagctcat tgattgatcc
     1621 ctcgctttat tcaatttctt ggtttattat agcacactct ttttaatata tcgtttgttt
     1681 ccgctatgac aaaatccagc tttattcaaa ttttcttgac acaggggaca agcggatgag
     1741 tattttcata aacaatgaac tgactgtaat agataggaga acgaatagtg tctccaacaa
     1801 tccaaaaaca taataattca aaaagacgat aagactattg gtaagtaaaa gtccttgcgg
     1861 cattggaagc cgaaaaaatt tgtggaaaat aatcggcggt atcgttatac cgccatttga
     1921 ggccccgact gtgtacaatg tgtaaacccc aagactgaac aaaagacttc ctgaaatcag
     1981 tgaaaagaaa tgtgaggaga tcatttccac ttttggaaca atctctaaaa caacaggaaa
     2041 aagcagactt cccgctacag tcttaaaaaa tactgatttt cctaatagga cagcggctag
     2101 aagcagcatc ataagattga tgctccaaac agtaactgag cgttggatag aaaaggccgt
     2161 ttcaactaaa tgtccgatgg cgcccactcc ggcggaagct atatcgtgag ggagcaagaa
     2221 ccaattcaat ccaactgtga taaagaaaag acctaaaaat ataacgggta gtttttttat
     2281 ttcttgtgtc attgatatct cctataaatg ttagccataa aaggctgtat atttctttac
     2341 tttaccaaaa aaacgaaacc gaattcaaaa aaacaattgt tcttataaaa gttatataaa
     2401 atcgattgcc agtttctgtt ttaccaatta aaatgatagt aatgttttgt agaaagttgg
     2461 aatctgaccg atggataatc aaagaaaacc agcattagat caattaacac cgcaagaaaa
     2521 aatggcacga ggctctgctt ggatgaccgc cagtaatatc atttcaagat tgctaggcgc
     2581 tgtctatatc attccctggt atgcttggat gggcgaaaat gctaaggctg ccaatggatt
     2641 attcaatatg ggatacaata tatatgcatt gtttctactg atctctacag caggaatacc
     2701 tgctgcgatt gcaaaacaaa ctgcacgcta taattcattg aatgaatatg gcacttcacg
     2761 acgattattt atccgtgcat tacagatgat gggcgggtta ggcctgttgt ttgctttgtt
     2821 catgtacatc gcctctcctt ggctagctca tgcttctggt ggcggagaag aactgattcc
     2881 aacgatgcgt tctttgagta tcgctgtatt ggtttttcct tgtatgagtg tgatacgagg
     2941 gtattttcaa ggcaatcaag aaatgatgcc ttatgcgctg tcgcagattg tagaacaggt
     3001 ggcacgt
//
LOCUS       DADXTH010000138         2993 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.142, whole genome shotgun sequence.
ACCESSION   DADXTH010000138 DADXTH010000000
VERSION     DADXTH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.142"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            208..525
                     /locus_tag="IWI97_002698"
     CDS             208..525
                     /locus_tag="IWI97_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBC2464162.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(616..981)
                     /locus_tag="IWI97_002699"
     CDS             complement(616..981)
                     /locus_tag="IWI97_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464163.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1188..1670
                     /locus_tag="IWI97_002700"
     CDS             1188..1670
                     /locus_tag="IWI97_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464164.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1867..2757
                     /locus_tag="IWI97_002701"
     CDS             1867..2757
                     /locus_tag="IWI97_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBC2464165.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1028 a          464 c          566 g          935 t
ORIGIN      
        1 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
       61 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
      121 cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat tttttcggta
      181 tttaatattt aattaggagg taatattttg cttttttata gtaattttat cttaatagta
      241 gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaattc aagtataggt
      301 tttagaacta aacgaagttt gtcttctaaa aaaaattggg tatactcaca aactattttt
      361 tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt gaatattata
      421 gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc aattattaca
      481 caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa ctagtgatag
      541 ttttggtgat ataagaaaaa catatatgta taaaaagaag aaagtagctt ctctttgtac
      601 atatatggtt tgtttttatg gatgccgagc ttttttataa gataaggcta ccgcccataa
      661 cgacccaagg atttgtggaa ctaagtaaat acctgcaaga agcaagatca gctgggtcgg
      721 ttttaaagga tgatagaagt aacgccattc agtaaaattg gcgcaaaata aataggaagt
      781 gacagtagat ataatattgg caatgaataa agatataata cgcctgttcc tttgaaaata
      841 atatccagca aaaagagata aaaatatcaa tgtgatgaac ccgcttacgg aatgataacg
      901 aaaatcaagc agtaaggcga aatgcacata aggaacaaaa gataaaagca acggaaggtt
      961 tttccagcaa gtttttatca tctttcatca ccacctaaca agtgttatta ttttgttagt
     1021 tttattgtaa attgaatcct gcaataatca attacttttt cattatttct agattaaatg
     1081 aataaactct catgccatta tctctaaaaa aaagtttttc cccctaattt ttttagatag
     1141 aaaattgtat agtatgatga gagaaaaagt agtttggggg aacgttaatg agaagaaaag
     1201 cagtaatcga tcggctttat ttattttttt ggttttttcc tatcgttttg ctacctttta
     1261 catgggtaaa tgctgttgta caaggtgcgg agatattagg cctgatccaa ggaagcggat
     1321 ttgtggtttc taggaatttt ccagctttga ttgttttcta tttagtgata agtgtaggtc
     1381 tcttattacc aaaatctctg atcaatcact acaattggca gctgctttac ttttcttgct
     1441 tattagtatt tacatctatg ttttctttgc tgcctcgctt gattatcggc cctaatggtt
     1501 tgagcgagag tggattgaag gatttagctt attcctccat gtatttcttg acgatcaaac
     1561 catggttcta tttagtaatt gcctcattta ttcttgcaac atttttttac ttctggacag
     1621 aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa taactattga tggtggatca
     1681 tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat
     1741 tatgtaaaga atgcgaaaaa cgtttacatg atttttacat aacttaacta tatttttaca
     1801 gcaaccatac gagggattta cagtcgactg ataaacttta gttgtaaata agaaaggagc
     1861 aataaaatga aaaaagtatt gtttactcta ggtatgatgg gaattctttt agccggatgc
     1921 ggctcaggat caggaaatac agatggctct gccacacaaa ataattcaag tgactcaaat
     1981 gaacaagtaa aaatcgttgc ggtaggttcg acagcattac agccgttagt cgatgcagca
     2041 caagaaagct ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg aggaggaagc
     2101 ggaactggtt tgagccaagt cgaagctgga gcagttacta tcggaaactc tgatgtattt
     2161 gcagaagaaa gagacggagt tgatgcttca aaattagtgg atcataaagt agcagtggtc
     2221 gggatggcac caatcgtcaa taaagataca gatgtcaaag atatcaccaa acaggaattg
     2281 attgatatct ttacaggaaa aatcactaac tggaaggaag ttggcgggaa agatcaaaaa
     2341 atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga aaaatgggga
     2401 ctggatggtg ctacccctgt gcagtcccaa gaacaagatt catcaggaac agttcggcag
     2461 ctggtaagtc aaacaccagg agcaatcagc tatttagctt tctcatatct cgatgattcc
     2521 acacaagcac taagtatcga cggtgtagaa ccaaaagaag agaatgtagc agataacagc
     2581 tggggaatct ggtcatatga acatatgtac acgaacggaa aaccttcccc tgaagtccaa
     2641 aaattcttag actacatgat gacagaagaa atccaagaag gacctgtcaa agaattgggc
     2701 tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat caaataacta
     2761 gcagataaac atgccacggt agtcgttact attaaggaaa aatcaactat gtttccccac
     2821 atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt tttttgagct
     2881 aataccgagg gtcagctgat tgaacaccgt ttattcgaaa aaaagagcta cgacatagaa
     2941 ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg tgaagacaaa ttt
//
LOCUS       DADXTH010000139         2959 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.143, whole genome shotgun sequence.
ACCESSION   DADXTH010000139 DADXTH010000000
VERSION     DADXTH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.143"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..1461
                     /locus_tag="IWI97_002702"
                     /note="aph(2'')-Ia"
     CDS             22..1461
                     /locus_tag="IWI97_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBC2464166.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1549..2721)
                     /locus_tag="IWI97_002703"
     CDS             complement(1549..2721)
                     /locus_tag="IWI97_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HBC2464167.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT         1070 a          389 c          526 g          974 t
ORIGIN      
        1 tatgaaaaag gtgataaata aatgaatata gttgaaaatg aaatatgtat aagaacttta
       61 atagatgatg attttccttt gatgttaaaa tggttaactg atgaaagagt attagaattt
      121 tatggtggta gagataaaaa atatacatta gaatcattaa aaaaacatta tacagagcct
      181 tgggaagatg aagtttttag agtaattatt gaatataaca atgttcctat tggatatgga
      241 caaatatata aaatgtatga tgagttatat actgattatc attatccaaa aactgatgag
      301 atagtctatg gtatggatca atttatagga gagccaaatt attggagtaa aggaattggt
      361 acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt
      421 attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt
      481 agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat
      541 ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa atatttaatt
      601 gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag tggttatgat
      661 agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag tactaataag
      721 aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa tttagaaact
      781 aatgtaaaaa ttcctaatat tgaatattcg tatattagtg atgaattatc tatactaggt
      841 tataaagaaa ttaaaggaac ttttttaaca ccagaaattt attctactat gtcagaagaa
      901 gaacaaaatt tgttaaaacg agatattgcc agttttttaa gacaaatgca cggtttagat
      961 tatacagata ttagtgaatg tactattgat aataaacaaa atgtattaga agagtatata
     1021 ttgttgcgtg aaactattta taatgattta actgatatag aaaaagatta tatagaaagt
     1081 tttatggaaa gactaaatgc aacaacagtt tttgagggta aaaagtgttt atgccataat
     1141 gattttagtt gtaatcatct attgttagat ggcaataata gattaactgg aataattgat
     1201 tttggagatt ctggaattat agatgaatat tgtgatttta tatacttact tgaagatagt
     1261 gaagaagaaa taggaacaaa ttttggagaa gatatattaa gaatgtatgg aaatatagat
     1321 attgagaaag caaaagaata tcaagatata gttgaagaat attatcctat tgaaactatt
     1381 gtttatggaa ttaaaaatat taaacaggaa tttatcgaaa atggtagaaa agaaatttat
     1441 aaaaggactt ataaagattg attatataat atatgaaaag ctattataaa agacattagt
     1501 caagtccata atcctgtgta aaatactata caatgtttta ccggtctctc attgatcaaa
     1561 cttaatatat tttcttgtag aactgagcca ttcgtcatga aggtccataa ggacagctcc
     1621 aattaatcga ttggcagacg ttcggttggg aaaaatccga ataatctttt ctcttctgcg
     1681 gacttcctgg ttcagtcgtt caagaaggtt ggtgcttttt agccgattat gactatttcc
     1741 gataaccgtg tattgaaagg catcttcgaa gccattatcc aatatttcgc aggcttttgt
     1801 atatttaggc tggtcgatat attcaccgac taaggcattc ttagctgttc gagcgagttc
     1861 aatatccgta aacttaaaga tcgcttttac tgcttctcta aacggttttg aattcttttt
     1921 tggaatggaa ctgaagatgt ttcttaaaaa atggacctgg catctctgcc aacttgcgtt
     1981 ggtaaatgac ttacgaatcg cagacactag gcctttatgg gcatcagaaa tgatgagttc
     2041 tgtccccttt aggccgcgtt cttttaagta ttcaaagaag atggaccatg tgtcatcact
     2101 ttcttcattt tgaatcatga agccaatgat ttcacggtcg ccaccttctg ttatcccgat
     2161 cgcaatatgg cagcttttag aaagcactcg atggtcctca cggactttta tgtagagaac
     2221 atcagtcatc agataaggat aattcgtacc tgagagtgaa cggttctgcc attcgttgac
     2281 catcgggtct aactgctcag tcaggctaga aacaaaagat ttcgatacag attttccaca
     2341 tagctcttca acaatctttg aaactttacg tgtcgaaacg cccgaaacat acatctcaag
     2401 cattgaagcg agcagtgcct tttcatttcg ctgataacgc tcaaacaccg tcggtgaaaa
     2461 ttcaccatca cgtgttctag gcacttttaa ttcgagtgta cccacacgag tcgtaaagtc
     2521 tcgctcataa tagccatttc tttgactctg acgactttct gaacgttcat agtcatcggc
     2581 ttgaatatat tctgttcgtt gattttccat caattggttg aaaacagtgg ttaaaatatt
     2641 tttagaaaca tcatctttta ccgaatgttc aataatactt tgaacctctt cgttgttcag
     2701 tgtaaaatgt acttgggtca tgtaacgtcc tcctgggtat gtttttgtgg ttaaaaacat
     2761 tgtaccgtaa aagggctgtt acatggcctt ttatctttta cacaattata tggactttat
     2821 caagacatta gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tggaaagatg
     2881 gatttagaaa aaacaaataa taattcaatg attattaaat atcaaaatac agataatgta
     2941 ttagaagata tatgttcta
//
LOCUS       DADXTH010000140         2738 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.144, whole genome shotgun sequence.
ACCESSION   DADXTH010000140 DADXTH010000000
VERSION     DADXTH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.144"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(46..492)
                     /locus_tag="IWI97_002704"
     CDS             complement(46..492)
                     /locus_tag="IWI97_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBC2464168.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(728..1654)
                     /gene="cysK"
                     /locus_tag="IWI97_002705"
     CDS             complement(728..1654)
                     /gene="cysK"
                     /locus_tag="IWI97_002705"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBC2464169.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1794..2681)
                     /locus_tag="IWI97_002706"
     CDS             complement(1794..2681)
                     /locus_tag="IWI97_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBC2464170.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          775 a          605 c          447 g          911 t
ORIGIN      
        1 tacacagcta gcttttttta ggtataacac ttgtagaaac tgtttttaca tttgcgcctt
       61 ttttgctgac tctttacaat ccggacagag tccatacaat tccaaccgat gttcattaat
      121 ctcaaaatca gtcagtctgc ttgcagccat ttctatatct tcaagccctg ggtaatgaaa
      181 atcaacaatc ttaccgcagt tttcacaaat tacatgataa tgacgctttg aactgaaatc
      241 aaatcggcta gaagcatcgc cgtacttcat ttcttgaaca aatccaatcg aagtaaataa
      301 gcgaaggtta ttgtaaacag tcgcaacact catatttgga aaccgatgtt caagtgcttt
      361 ataaatctca tcagccgttg gatgggaatg atgttcaatc aaatattcta aaaccgcgta
      421 ccgttgaggt gtgattcgaa tgttctcttt ttttaacttt tcgataacct cttcgactaa
      481 cttattttcc atgtaaacac cccacgttgt tattctcgtc ttatcatact atttttacat
      541 tttaaaatcc acttaacatg aaaagaaaat tcaattttat tgtattttaa tgaaaaaaga
      601 cgtaaaacag tcgcgttcac tattctacgt ctctattcaa ataatttttc tcatcataaa
      661 tttttgtgtg ttcttttcat ctttaaaagt tttttacata aacctatctg gagaaaaacc
      721 aagtgcctta tatctcttgg taaagggctg tagataaata acgttcacca ttgtcgggaa
      781 ccacagctaa taccttcttg ccttttccta attcttttgc gacttttagc gcggcagcaa
      841 ttgcagcgcc agatgagatg cctactaaaa tcccttcttt tcgcccaact tctctagctg
      901 tttccatcgc ttcgtctcca ctaatagaaa ggactttatc atacacagaa gtatccagtg
      961 ttttaggaac aaaacctgta ccgattcctt ggattttgtg aggtcccgct tctttccctt
     1021 ctaaaatagc agattctgct ggttctaccc cgattaattc gattttcgga tacacacgtt
     1081 ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc gatacctgca acaaacgcgt
     1141 ccaagccatt tactccaaat gcttgatgta tctcaggacc agtcgtcttc tcatgcacaa
     1201 gaggatttgc ttcattttca aattgtaatg gtaaaaagta gccgttttct tttgccaaac
     1261 gttctgcttc tctgattgat ccagaaatac catccgcacc aggagtcaaa atcaactctg
     1321 cgccgtaccc tttcatcaat aaacgtcttt cgatactcat cgtctcaggc atcacgatga
     1381 ttactttata tccttttgca actcctacca tcgacaatcc gattcctgta tttccagaag
     1441 taggttcaat gatagtatca cctggtttca gtagaccatc gtgttctgct ttttctatca
     1501 tacttaaagc aatacgatct tttacacttc ctcctggatt aaaaaactca agctttacaa
     1561 aaacatctgc tgaatcttct ggtacgattt tatttaattt gacgattggc gttttgccaa
     1621 tcaattcggt gactgagtta tagatagctg tcatcttcaa ttcctccttg tctttgctct
     1681 tcttacttat cataatacaa tctgttcatt tatggcgagt gattcgtctt tgactagcaa
     1741 aaaaaagaca aagatatacc atatatggta atttctttgc ctttttatct aaatcaaacg
     1801 tttagttctt gaccaactaa taaagaataa ctggacatag acatgccatt tttcgccatt
     1861 agatcatcaa cagacatgcc aaatttttgt gcaatatccc atagcgtatc acctgacacg
     1921 actgtatatg tctgaccatt atttgtattt acatctgttg ttgaagatga tgacgttgaa
     1981 gtcgtcgtag tagtcgttgt tgttgtactt gtggttgttg tagccgaacc tgttgatggc
     2041 gtatcaaaac gggttaaatt ataagtatta atcaatgagt tcaatttggc cccataattt
     2101 ggatcggtag cataacgacc tgtcaagtac gctgttgcat cataaaaact tgttgtattg
     2161 cttttccaca cgccagcata atgataatcg cccgtggaaa aactggttga acgaagaaca
     2221 ttcgcatggt cttggaagga ttcccaataa gaagaatatt ttcggaatgg ctcattcatc
     2281 gttacccatt gtccgtttac atattctttg gttgccatat aaacgacctg tccgttatag
     2341 cttcctttca caccgaatag attataattt ggcgcactag ctaatccgga agtgccatat
     2401 gcactttcca acaacgcttg agcgatcatg actgaagcat acaagtcatt acttgcagca
     2461 acttctgacg cagaccagcc gatttgatcg ataaacgctt gaggatcagc gactgtttga
     2521 gatccttgtt catctgcatg aacatgttgc ggtaccgctg taaccatcat cggcccaact
     2581 aaaagactcg ttccaattaa aggagcaatc gcttttttat tgatcaccat ttttctttta
     2641 ggtctatggt gccgggcact gcgggtcttc agctcttgca ttcaaattcc tcctcaaaat
     2701 aaaaaaactt aataaaaagt tctcttctta aaacaaac
//
LOCUS       DADXTH010000141         2723 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.145, whole genome shotgun sequence.
ACCESSION   DADXTH010000141 DADXTH010000000
VERSION     DADXTH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.145"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(488..805)
                     /locus_tag="IWI97_002707"
     CDS             complement(488..805)
                     /locus_tag="IWI97_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBC2464171.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(806..1060)
                     /locus_tag="IWI97_002708"
     CDS             complement(806..1060)
                     /locus_tag="IWI97_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBC2464172.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1320..1538)
                     /locus_tag="IWI97_002709"
                     /pseudo
     CDS             complement(1320..1538)
                     /locus_tag="IWI97_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1804..1998)
                     /locus_tag="IWI97_002710"
     CDS             complement(1804..1998)
                     /locus_tag="IWI97_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464173.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(2098..2184)
                     /locus_tag="IWI97_002711"
                     /pseudo
     CDS             complement(2098..2184)
                     /locus_tag="IWI97_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(2590..>2723)
                     /locus_tag="IWI97_002712"
     CDS             complement(2590..>2723)
                     /locus_tag="IWI97_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2464174.1"
                     /translation="VNQTFISSVSNQRNHIPRESLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          885 a          487 c          406 g          945 t
ORIGIN      
        1 aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta ttcccaaaaa
       61 tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa ttgacgtaac
      121 ttttaataaa tactacctaa ataaggtagt atttattttt gtgttgtgaa ctactcacca
      181 cttagctaaa cctgacggtt cttaacgctt gaagtgggag cttcttggga atagagcata
      241 cttgatagcg tatttcttta ctaacagcgg tgtctcttat ttaccaagct atccccgtag
      301 ttcctacggt tcttaatttt tctctagact aatgattgta tctttagacc ttcggccata
      361 acatggagca aaataaaaag gtactaagtc ttatatatca atcttcgagt aaccgacggt
      421 taacaaccat atcgagcggc attaatcacc cacctgtaga ggatgagcga cttttgccta
      481 attattgtta aaagcctagt ttcatttgtt tagattggaa aatacgtcct acaagtatct
      541 cttttttttc atggtcgatc cgataaaata aggcatagct tttgccgatc acgatccgat
      601 atgttgggat atccattcca tagatttcgg aaacttcctc atgcatagtt ggaaaatttt
      661 tggttagttc taaagccttc tggatcgcac ctacaaatct ttttatgctg gctggagaaa
      721 gagcaagctc attttcccat tcttctatta tcgtttcaag gctggtctgg aaacttttag
      781 aataaactag cgtgtactct ttcatctatt cactccagcc gtattttccg aaagctttta
      841 ccatttcaat ttctgaaact gttaaacctt tctcaaagtc ttggaaactt tcttcaatca
      901 atttgcgatc gtgttctttt gctatttgag tagtatctaa tatgaataag ttgccttctt
      961 gtttggcttg gatagtgtct ccgcttttta atccaagaga gcttggaagg atcactccca
     1021 cgctattgcc gatctttcta acctttagtt cttttcccat gttatcacac tccttttagt
     1081 ttttgatgaa taaattataa catgttataa caacaattgc caacggtgtt tcttaaggga
     1141 ttccactcca tattattatt ggacaaaata agccacttag aacgctgaaa acatatacaa
     1201 acgatatcaa aatacgagtg aactactcac catacacttc cgttaacatc catttaagta
     1261 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
     1321 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
     1381 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
     1441 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
     1501 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
     1561 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
     1621 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgcatcta aaaatatata
     1681 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
     1741 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
     1801 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
     1861 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
     1921 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
     1981 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
     2041 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
     2101 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
     2161 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
     2221 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     2281 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     2341 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     2401 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     2461 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     2521 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     2581 tatgatttgt caaattaagc tagaataaaa tgcttcttgt acatagctca aaaatatttc
     2641 gattggtgtt ctgtaattca atgattctct tggaatatga ttacgttgat tgctgacact
     2701 ggaaataaat gtctgattca ctt
//
LOCUS       DADXTH010000142         2708 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.146, whole genome shotgun sequence.
ACCESSION   DADXTH010000142 DADXTH010000000
VERSION     DADXTH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.146"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(75..1169)
                     /locus_tag="IWI97_002713"
     CDS             complement(75..1169)
                     /locus_tag="IWI97_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBC2464175.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="IWI97_002714"
     CDS             1358..2119
                     /locus_tag="IWI97_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBC2464176.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="IWI97_002715"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="IWI97_002715"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBC2464177.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          856 a          542 c          463 g          847 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccatttt
//
LOCUS       DADXTH010000143         2625 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.147, whole genome shotgun sequence.
ACCESSION   DADXTH010000143 DADXTH010000000
VERSION     DADXTH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.147"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..937)
                     /locus_tag="IWI97_002716"
     CDS             complement(62..937)
                     /locus_tag="IWI97_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2464178.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1192..1276)
                     /locus_tag="IWI97_002717"
     tRNA            complement(1192..1276)
                     /locus_tag="IWI97_002717"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1240..1242),aa:Leu,seq:caa)
     gene            complement(1294..1365)
                     /locus_tag="IWI97_002718"
     tRNA            complement(1294..1365)
                     /locus_tag="IWI97_002718"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1331..1333),aa:Gln,seq:ttg)
     gene            complement(1375..1447)
                     /locus_tag="IWI97_002719"
     tRNA            complement(1375..1447)
                     /locus_tag="IWI97_002719"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1412..1414),aa:His,seq:gtg)
     gene            complement(1509..1593)
                     /locus_tag="IWI97_002720"
     tRNA            complement(1509..1593)
                     /locus_tag="IWI97_002720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1557..1559),aa:Leu,seq:caa)
     gene            complement(1614..1684)
                     /locus_tag="IWI97_002721"
     tRNA            complement(1614..1684)
                     /locus_tag="IWI97_002721"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1650..1652),aa:Cys,seq:gca)
     gene            complement(1730..1801)
                     /locus_tag="IWI97_002722"
     tRNA            complement(1730..1801)
                     /locus_tag="IWI97_002722"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1767..1769),aa:Gln,seq:ttg)
     gene            complement(1813..1885)
                     /locus_tag="IWI97_002723"
     tRNA            complement(1813..1885)
                     /locus_tag="IWI97_002723"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1850..1852),aa:His,seq:gtg)
     gene            complement(1888..1961)
                     /locus_tag="IWI97_002724"
     tRNA            complement(1888..1961)
                     /locus_tag="IWI97_002724"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1927..1929),aa:Trp,seq:cca)
     gene            complement(1968..2048)
                     /locus_tag="IWI97_002725"
     tRNA            complement(1968..2048)
                     /locus_tag="IWI97_002725"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2012..2014),aa:Tyr,seq:gta)
     gene            complement(2050..2125)
                     /locus_tag="IWI97_002726"
     tRNA            complement(2050..2125)
                     /locus_tag="IWI97_002726"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2090..2092),aa:Phe,seq:gaa)
     gene            complement(2161..2233)
                     /locus_tag="IWI97_002727"
     tRNA            complement(2161..2233)
                     /locus_tag="IWI97_002727"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2198..2200),aa:Asp,seq:gtc)
     gene            complement(2243..2315)
                     /locus_tag="IWI97_002728"
     tRNA            complement(2243..2315)
                     /locus_tag="IWI97_002728"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2280..2282),aa:Val,seq:tac)
     gene            complement(2331..2402)
                     /locus_tag="IWI97_002729"
     tRNA            complement(2331..2402)
                     /locus_tag="IWI97_002729"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2367..2369),aa:Glu,seq:ttc)
     gene            complement(2417..2505)
                     /locus_tag="IWI97_002730"
     tRNA            complement(2417..2505)
                     /locus_tag="IWI97_002730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2469..2471),aa:Ser,seq:gga)
     gene            complement(2545..2618)
                     /locus_tag="IWI97_002731"
     tRNA            complement(2545..2618)
                     /locus_tag="IWI97_002731"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2582..2584),aa:Asn,seq:gtt)
BASE COUNT          785 a          604 c          496 g          740 t
ORIGIN      
        1 taatcatagt ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat
       61 tttaatagat tcgtgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa
      121 gttgaaattg ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat
      181 gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca
      241 agcgtttaac caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat
      301 cataaacgtt tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta
      361 tttttaagct ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga
      421 tgaatcctac ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca
      481 cttctattgc tatatcatat tcatttggat aaaggaatct catttctggc aacataattg
      541 ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg
      601 taatatagat atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt
      661 cttccgcaaa atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga
      721 tcttttcttt agaatctaat acaaataatt tgtcagcatc ctcaacatca aatggcttac
      781 tcactttagc agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat
      841 cgatcaatac agcattatga ttcagctttt tcatcacata caccgttaat tcacctccac
      901 tatatgataa aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat
      961 ttttaaagac aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata
     1021 gtagcaccct acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc
     1081 ttttttactc gttttatgat aatacttgta tcgaattgat ttggaggtaa ttacataaaa
     1141 acaaaaaaaa tatagcaact ctctataaga aagttgctat attgataatt ataccggcgg
     1201 ccggggtcga accggcacgt cctcaacgga cactggattt tgagtccagc gcgtctgcca
     1261 attccgccac gccggcaaat ttaaaaataa taactggggt agctggattc gaaccaacgc
     1321 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaagggcgaa
     1381 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
     1441 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
     1501 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
     1561 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
     1621 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
     1681 cgccattata ctattatcaa tctacaaaat aaagaataaa ttatagaaac tggggtagct
     1741 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
     1801 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
     1861 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
     1921 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
     1981 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
     2041 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
     2101 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
     2161 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
     2221 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
     2281 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
     2341 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
     2401 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tgcaccatct
     2461 acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct
     2521 caatactctt tctcaaaatg agaactccgg cagtaggact cgaacctacg acatcatgat
     2581 taacagtcat gcgctactac caactgagct atgccggaat aacat
//
LOCUS       DADXTH010000144         2483 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.149, whole genome shotgun sequence.
ACCESSION   DADXTH010000144 DADXTH010000000
VERSION     DADXTH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.149"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            281..973
                     /locus_tag="IWI97_002732"
     CDS             281..973
                     /locus_tag="IWI97_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBC2464179.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1180..1470
                     /locus_tag="IWI97_002733"
     CDS             1180..1470
                     /locus_tag="IWI97_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464180.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          943 a          349 c          458 g          733 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt tagtacatgt ccttcatctt cgcaggaagt gccaatatga
     1081 ttttaagaaa ggtaatagac aaaaagtcaa aaagataaaa cagagtttct tctttacaaa
     1141 gggaatgtat gttcgtataa tggtattgag gtgaaggtta tggagaaaaa atatggaaca
     1201 gtagataaga tccgtacttt gcaagttagt gccagaccgt tgatccgttt cagtttaaat
     1261 ggggtcaatt gtttgattgc ttcccatagt ttgaacttcc tagctgaagt agacgaaggg
     1321 atgaagttag tcgttacagg ttattataat aatagaaagc aatttgttgt gagatcctat
     1381 catgtgttag ggaaacccaa aattgtggta gaatatgaga aaagtctata cccaagaaaa
     1441 aaggtccgac aagatgaaag aataaactag aaaacaagcg gctgagacaa aaatgtccta
     1501 gccgcttgtt ttggaaagat ggatgcagac gatgggattt gaacccacac gagcatacgc
     1561 tcacaagaac ctcaatctat atataactac atgtatttaa gtatttgtga tgaatcacta
     1621 aactttccaa actcatttga gggatattgc ttaaagattg aacagatcaa ttcgaagccc
     1681 tattagtatt cgctcaatgg ggggatcaga ggccataaaa agagactagt cattactagt
     1741 ctcaggtagt ggaattgtag gattttaaac gttcctggag agtaccggta tgtaactcga
     1801 agaggtgatt atcatagtcg taaaagtaaa ttgaagaagc ctcgcctttc acacgagaac
     1861 gtcctggttc gacagttaaa cctaacattt ggatttttga gacaaacgaa tctatatctt
     1921 gttcgtcaat tttaaaagca atatgattgt atgtctttgg cagtttattc gacgaattct
     1981 gcattactgc aatccatagg tccttaacta agaaaaattt ttctggatat aatgaaaatt
     2041 tttttgttga ctatcgtaga tttcttttgc atcaaataag gtatttaaca attaagtatt
     2101 taaaagataa cttttaagtt atctttaagt aatgcttaaa gctatgttat actctattag
     2161 gaaatataag aaggagtata aaaatgaaca aacataatag aaaaataacg aataacaaag
     2221 aactgatctt gttactaata gggatttggg gtatcttttt actctttaat atgtatatgc
     2281 cgactatgag agcggatgac gtagtttatg caagtagatt agatgaactt ggctatttgg
     2341 gtgcttcgat agaacattat aagacatgga gttcaagaat tattatagaa ctattcttaa
     2401 tgttcttttc taaacacttt atgctatgga aattattaaa ttctacgatt atgttaggaa
     2461 ttgtagtatt actttgcaag tat
//
LOCUS       DADXTH010000145         2215 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.152, whole genome shotgun sequence.
ACCESSION   DADXTH010000145 DADXTH010000000
VERSION     DADXTH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.152"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IWI97_002734"
     CDS             complement(<1..142)
                     /locus_tag="IWI97_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2464181.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(653..1195)
                     /locus_tag="IWI97_002735"
     CDS             complement(653..1195)
                     /locus_tag="IWI97_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464182.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(1508..2059)
                     /locus_tag="IWI97_002736"
     CDS             complement(1508..2059)
                     /locus_tag="IWI97_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2464183.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
BASE COUNT          652 a          423 c          400 g          740 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccaact taagattaat gacactaaat
      241 taacagacta tacgtagtat ctatttgcta tgaccatatc ttatatccat aagtcaactc
      301 agccggacta aaagggctaa tcttactaga acacaagctg cacttgatat gcaaattaac
      361 cctgaatcgg aaaggtgctt aaattggata ttaacggagg gataaagtaa aatttatctc
      421 tagggaatgt ttagaaagga tccaagttcc ttaacaagtg acaattagta atgtagtaac
      481 aacgatagat tgtatgccga acatttcaat ttgatacgta taaaagacga tttcaagctc
      541 atccgtgata aataacccac atgaatgatt tgaaatcgtc ttaaaatgct ttattttata
      601 acccaaaata gaataattac ttcaaaatga cgtcgcatat cactattaat gattaatcga
      661 tgacttccat aatcatttta agttccccgg atgttaaatc ctcgctaaaa atgccagtaa
      721 ctttttgtcc aggtccaatt tcctttgatg ataggtttaa caaagagata gggatataga
      781 ttaatccgtt ttgtttcaaa gacagagcca atattttttt ccttgttcga atagctgcat
      841 catagcgaag agaagagtaa acacctctgc caacaataga aatatgcttc cccaataata
      901 aatcaaggct acctattact tgtaatttct gaaaggtcgt cccatccgtt ttgatttgaa
      961 tatcctgtaa atttattttc ctgtctaagg catcttgaaa aaacaagtgt gtgcctctgc
     1021 gataatcaat gccacagagg tgcataaaat tagttgtcga aaaaataatc gctaccgttg
     1081 agacttctcc gacaaaggat acttttttat ctacaaaatg ttcttgtaag agatgagcag
     1141 ccttataaat cgcgtttaac tgtctggtaa tttgggttgt ctcttttata gacatgcgtt
     1201 tgtattcttt ttttgccgcg tgcgaagaat gctccttttt cccaataaaa aaagctgctc
     1261 aaaagagcag ccttttgtac aggtttagtt ttggtgcttg ccacccgccg gacccttacg
     1321 ttccggattt gtatcgagtt tagttttggt gctcgccacc cgccggaccc ttatgttccg
     1381 gatttatatc aagttgacta tgagcctgct ctcatttgta tatataatat accaaaaaaa
     1441 ggttgatttg tccaatactg tgggtgatta taaccgtctt actgtgctta tctattatat
     1501 aatacaatca aaaacctaac cgaaaattta ataacgtttc actgatttca tcttcattaa
     1561 ttccgatgta gcgtttggta atcttctcgc tgctgtgacc aaagatttcc attaaggtgg
     1621 ccacgtcttt ggttttcttg taatagtggt aaccaaaggt ttttcgtagg gtgtgggtgc
     1681 cgatatcgtc ccttcctaac agcttcgcga ccttttgaaa catctggtag accgtattga
     1741 cttctaaatg gccgccctta gtactgggaa acaagtaagc ctccggatct aagggtgcgg
     1801 tatagccgtg aatcaagtcc tgtaagctgc ttaagtataa aatgcgtgtt tttcctgtct
     1861 tttgctccac gatgcgcggg ttttttgagg taatcacgtc ttttttcttg agtttaacga
     1921 tgtcggacat gcgcagaccg ctgttaattc caattagaaa caagaaaacg tcacgttccg
     1981 catttttatt ccgtcgcagg caaaataaaa aatcattaat ctcctgttgc gaccgtagtg
     2041 gctgaacatt atagcccatg acaatccctc ctttttagtc tgttcgaata catgattata
     2101 gttattacaa cacgaaatcg tgtgtttagg gggagtaaaa aaaaagaacc ctataaggat
     2161 aggattcttg atacatgatt ttatgtaatt tcatgtattt aggtccgata ttatt
//
LOCUS       DADXTH010000146         2137 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.153, whole genome shotgun sequence.
ACCESSION   DADXTH010000146 DADXTH010000000
VERSION     DADXTH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.153"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            68..484
                     /locus_tag="IWI97_002737"
     CDS             68..484
                     /locus_tag="IWI97_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HBC2464184.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            504..2048
                     /locus_tag="IWI97_002738"
     CDS             504..2048
                     /locus_tag="IWI97_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2464185.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
BASE COUNT          757 a          371 c          490 g          519 t
ORIGIN      
        1 ttccagggat gagctgacat tcaatttgaa atgcggtctt tccctgaaag aaaaggtggt
       61 gagataaatg gcatatattc catatggata caaaattcaa gatggagtgg ttactgtcga
      121 tgaaaaggca gcaggtcaag taaaggtatt ctttgagaaa tacatatcag gactatccct
      181 tacagtggct ggcgaacagg caggtattga aaagacacat tctgtgatgg ggcgcatttt
      241 gaaaaacgtc aactaccttg gaaatgatac gtatccagca attattgata aagagatatt
      301 tgataaagct gaagaagtta gagataaacg tgcaaaggat ttaggacgag tggtagagct
      361 tgccgctttc acctctcccc ctcccaaaga acgattcaaa atgaaaaagg cagataataa
      421 gatgccagtg gatccttttg aacgagcaga atacttatac agtctgatag aaagcgagga
      481 ataaagtgac agagaaaaat ataatggtta ttcctgctcg taaaagagta ggaagtacag
      541 ccgcaaaaga aaagataaag aaacttcgtg ttgctgccta ttgccgtgtt tctacagaaa
      601 cagaagaaca aaattctagc tatgaggttc aggtctcaca ctatacagag tttataaaga
      661 aaaataatga atgggagttt gctggcatct ttgcagatga tggtatctct ggtacgaaca
      721 ctaaaaagcg tgacgaattt aatcgcatga ttgcagagtg tatggacggt aacatcgaca
      781 tggttattac aaaatccatc agccgatttg cgcgtaacac cctagactgc cttcaataca
      841 ttagacagct caaggataag aacatatcag tttattttga gaaagagaac atcaacacca
      901 cggatgccaa aggtgaggtt ttgctgacta ttatggcatc tttggcacag caggaaagcc
      961 agagcctttc acaaaacgtt aagcttgggc tacagtaccg ataccaacaa ggaaaggtgc
     1021 aggtcaacca taagcgattt atgggctaca cgaaagatga agatggaaat ttaactattg
     1081 tccccgaaga agctgagatt atcaaacgca tctaccgaga ataccttgag ggtcagagtc
     1141 tagtgggcat tggtcgagcc cttgaaaagg atggtatttt aacagcagcg ggaaaaccaa
     1201 gatggcgacc agaatcagtt aagaagatac ttcaaaacga aaaatacatc ggagatgccc
     1261 ttctgcaaaa gactgtcaca gtagattttc tgaccaagaa acgagtgaaa aatgaagggc
     1321 atgttcccca atattatgtt gaaaatagcc atgaagcgat tattcctaaa gagttatttt
     1381 tgcaggcgca agaagaaatt catcggagaa gcaatatcta cacaggagaa ggcaagaaca
     1441 aaagaattta cagtagcaag tacgctttaa gtgccatcac cttctgtgga gattgtggcg
     1501 atatttatag acgggtctat tggaatatcc acggaagaaa agaatttgtc tggcgatgcg
     1561 tgactagaat cgagcaaggt cctgaagttt gtaagaaccg aaccgtaaaa gaagatgaac
     1621 tttatggtgc agtaatgact gcgattaata agctacttgc aggtgggaac aatatgataa
     1681 agacactgga agagaatatt catgctgtga ttggcgaaac cacagaatac caaatttcag
     1741 agattaacaa ctcactagag gaaaaacaaa aagaactaat caagctggcg aacaagggtc
     1801 aagactatga ccacttagca gatgagattg atgagctgag agacaagcga cagatccttt
     1861 tagtagaaga tgcctccctc agtggcgaga acgagcgaat caatgagctg attgaattta
     1921 tccgcaagaa caaattccgc accttagaat atgatgataa gcttgtaagg aagataatcc
     1981 agagcgttac agtctatgaa ggccacttcg tcatagcctt caaatctggc atcgaaatgg
     2041 aaatatgaaa accagaagta aatagcccat gactctacag tagagttgtg ggttttcttt
     2101 tgcttgctga ttataataat attttaataa ctattgt
//
LOCUS       DADXTH010000147         2011 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.154, whole genome shotgun sequence.
ACCESSION   DADXTH010000147 DADXTH010000000
VERSION     DADXTH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.154"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWI97_002739"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWI97_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBC2464186.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWI97_002740"
     CDS             852..1040
                     /locus_tag="IWI97_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBC2464187.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWI97_002741"
     CDS             1053..1610
                     /locus_tag="IWI97_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBC2464188.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWI97_002742"
     CDS             1642..1884
                     /locus_tag="IWI97_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBC2464189.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          734 a          309 c          407 g          561 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta a
//
LOCUS       DADXTH010000148         1992 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.155, whole genome shotgun sequence.
ACCESSION   DADXTH010000148 DADXTH010000000
VERSION     DADXTH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.155"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..225
                     /locus_tag="IWI97_002743"
     CDS             <1..225
                     /locus_tag="IWI97_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBC2464190.1"
                     /translation="MDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLES
                     RFESILLAYSVLLSRARRRFEGTLRYSLGY"
     gene            complement(639..899)
                     /locus_tag="IWI97_002744"
                     /pseudo
     CDS             complement(639..899)
                     /locus_tag="IWI97_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
     gene            complement(910..>1992)
                     /gene="tndX"
                     /locus_tag="IWI97_002745"
     CDS             complement(910..>1992)
                     /gene="tndX"
                     /locus_tag="IWI97_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific resolvase TndX"
                     /protein_id="HBC2464191.1"
                     /translation="PENKKKLIVDPETAPIVKRIFELSQQGTSTHQIARILCKDGILV
                     PRAYRAKKKGILEQSKGFTFPTDWVGKNVKMILENQVYLGHMVSHKSQTKSFKNKKIV
                     PVPKEDWIVVENTHEAIIDKETFEIVQKFISVKKQPNKTGKPNIFVGLVKCPDCGRNL
                     AYSNPNGNEPRFRCRTYARNTELCTTHAISYKALYEIVQNDIKNHVNSMEELGDHFIK
                     EMRELSETSGSQKIHQFNQELVSLEKRIDEIDSIFTKLIEQNALGKITDERFSKMSMN
                     YETEQAEVVQSYRELKSKIKSEEEKVQGTDRFLETISKYKDVTELNRSMLCELIDSIY
                     VYQAEGIGKERTQKVEINYRFLAVSH"
BASE COUNT          594 a          368 c          280 g          750 t
ORIGIN      
        1 atggataaat cagaaaaact agaccacagc ttacttggaa aacaaagaaa aacagttgag
       61 actgtttttt cttccttaga aaaattaggt tgtcaaaatt tcaactcacg ttctgttaaa
      121 gggctagaga gtagatttga aagcatatta ctggcttaca gtgttctatt aagtcgagca
      181 cgacgacgtt ttgaaggaac tttgagatat tctttaggat attaaaatta actagcacca
      241 cgggtgttta tcttttaaat ttaacgggaa aacggggaat ctttacttaa tatggacaca
      301 tagaaagtat gacaaatttt ctatgtatcc tgtattaatt ccgttttata tactttagtg
      361 aaataaataa tcctttacta agtaccaaag tacctaaagt gacaattttt tatttcactt
      421 tacttttctc actcattttt tatcaagtat attttggtca ctttaggtac tacatatttt
      481 tatcataaaa caaaaaaaga attcatatgt tatttatata cctattttga aaaaacaaag
      541 actcatagaa agtccttcat actttctatg agtctctatg ttagctcttt tcttattgta
      601 agaataattg tgtagaaata cattaaacct aaacagattt atagatcatc aatcgtatac
      661 tctgaatacc cctcataaaa ataacttaca tcaattcctt tcataaatag ttgacgatca
      721 tggatttgtt ctgttagtgc ttgagaaagt aaatgactaa tttctaaatc gttgattgga
      781 ctacgttcca tagcagataa atagtcttcc ttatctacta aattccaatc aattaccttt
      841 tgtagttctt tctttaacat aagatccaac caaatccgca tacttcgccc atttccttca
      901 gagaatgtat caatgagaca ctgctagaaa cctataattg atttctactt tttgagtacg
      961 ttctttaccg attccctctg cttgatacac gtaaattgaa tcaatcagct cacacagcat
     1021 agaacggttt aactctgtaa catctttgta tttactaata gtttcaagga atctatccgt
     1081 accctgtact ttctcctctt cggattttat ttttgacttt aattctctat agctttgcac
     1141 tacctctgct tgttcagttt catagttcat agacatttta gagaatcttt catcagttat
     1201 tttccctaaa gcattttgct ctataagttt ggtaaaaata ctgtctattt catcaatccg
     1261 tttctctaag gaaactaatt cttggttgaa ttgatgaatc ttctgacttc cgctcgtttc
     1321 acttagctct cgcatttctt tgataaaatg gtctcctagt tcttccatac tatttacatg
     1381 atttttaata tcattttgaa caatttcata taaagctttg tatgaaattg cgtgagtagt
     1441 gcaaagttcg gtatttcttg cgtatgttct gcaacggaat cttggttcat ttccgtttgg
     1501 attggaatac gctaaatttc ttccacaatc tggacactta accaatccca caaagatatt
     1561 gggtttccct gttttgttag gctgtttctt tacactaata aatttttgta ctatctcaaa
     1621 agtttcctta tcgataatag cttcatgagt attctcaacc actatccaat cttcttttgg
     1681 cacaggaacg attttcttgt tcttgaatga tttggtttgt gatttatgag ataccatgtg
     1741 tcctagataa acttgatttt ccaaaatcat cttaacattc tttccaaccc aatcagtagg
     1801 aaacgtaaat cctttgcttt gctccaaaat cccttttttc tttgctcgat aggctctagg
     1861 aactaaaatt ccatctttac aaagtatcct tgcgatttga tgtgtacttg taccttgttg
     1921 tgataattca aaaatccgct tcacaatcgg cgctgtttca gggtcaacaa tcaatttctt
     1981 tttgttctct gg
//
LOCUS       DADXTH010000149         1978 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.156, whole genome shotgun sequence.
ACCESSION   DADXTH010000149 DADXTH010000000
VERSION     DADXTH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.156"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..424)
                     /locus_tag="IWI97_002746"
     CDS             complement(<1..424)
                     /locus_tag="IWI97_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBC2464192.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="IWI97_002747"
     CDS             complement(533..1564)
                     /locus_tag="IWI97_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2464193.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          610 a          326 c          340 g          702 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttatt
//
LOCUS       DADXTH010000150         1967 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.157, whole genome shotgun sequence.
ACCESSION   DADXTH010000150 DADXTH010000000
VERSION     DADXTH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.157"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(388..1779)
                     /locus_tag="IWI97_002748"
     CDS             complement(388..1779)
                     /locus_tag="IWI97_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBC2464194.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT          624 a          434 c          339 g          570 t
ORIGIN      
        1 aagtcacaac ctctggtaaa cggtgttctc ctgacaactc ctcggtcaca agacacaata
       61 ttcaacaaac atgagaagct attttttgaa attgctcttt gtgactcaga gtgaaacggc
      121 aggctcacga cctctttctt aaacggtgtt caaaagcttg catctgcggt aataagctca
      181 accaaacgta aaatatgagg aactattttt gtttggctgt tcttattact tgatgcaggg
      241 cagctttttc acaacctctt attcacggtg tttcagaagt agcttctcgg aaataagccg
      301 aaatttcaca aaaatttgag aaactatttt taggaaactt cttcttattt cttaaagctg
      361 accacttcta tcacaacctc tttttgacta tgcattcaca tcgatcactt tagaatcatc
      421 attgatgaca tcgcgatcta aatctttgat gcgtgcactt gtcacgatac cagctaccat
      481 gctaccgctg acgtttactg ctgtacgtgc catatcgatt aatggttcaa cagagataac
      541 taacccaacg attgctactg gcaagttcat tgcccctaaa acgatcagtg aagcaaatgt
      601 tgcgccccca ccgactccag ctacaccaaa agaactgatt gttacaattg ccacaagcat
      661 caaaataaat tccaaactaa agacattcac tccagctgct ggtgcgacga tcgttgccaa
      721 catagctgga taaacgccag cacagccgtt ttgtccaata gacaaaccaa aacttgctga
      781 gaaattagct gttgcatcat ctactcctaa agcttttgtc tgagtctcga tattcaatgg
      841 caaagctcca gcacttgaac gtgaagtaaa cgcaaagctc aataccggaa aggctttttt
      901 gaaatagttg attggattga cttttgcacc aatcaaaatc agtgagtgga caatcaaaac
      961 agcaagcaat gctacgtaag aagcaacgac aaatttacct aaattgacga tagctgcaaa
     1021 atcacttgtt gccaaaacgt ttgtcatcaa agcaaatact ccataaggtg tcaaacgcaa
     1081 aacaagcgtc acgatccgca taatgatttt atacagactg tcgactaaat ctgaaaagaa
     1141 tgctgcttct tttggcgctt ttcttttcac gcctagataa gcgattccga caaatgccgc
     1201 gaaaataacg actccaatcg tacttgttgc acgactacct gctaaatcag caaaaacgtt
     1261 tttagggata aaggataaaa tctgctgtgg aatcgataag ttttcaactg tttcctgacg
     1321 tgttgccagt tcagagattc gtgctgtttc tgcagctcct tgtgtgaatt tagctccttg
     1381 gagtccgaac agcattgttg aaccaattcc gatcaatgct gcaatagctg ttgttccaag
     1441 taaagaagtc aatacagtcg cactaatttt ccctagcttt tcagaaactt tcatttttgt
     1501 gaatgcacca atgatcgaaa taaacactaa aggcataact agcatttgca agaatgatac
     1561 atatccttga cctacaatcc cgatccattc cattgattga gtaacgactt tgtcgtctac
     1621 accaaagatc aattgaagca gaccgccaaa gacgatacca actcctaatg ctgtaaagac
     1681 gcgtgttgaa aattttgtat ggcgcttttg catacgatag aacacatata ataatgccgc
     1741 aaaaactaga accactaaaa ctgtgatgag tgttgtcatt tttctcctcc tcatattatt
     1801 aatgacaagt ccatcgaagg aagtaaaggt tcccgagaat gattcttcaa ccatcctcct
     1861 gaaaaaattt ttttcagtga cttttatatt ttagacgatt tttatcgaaa tgacattctt
     1921 tttgcttaca attagtaata aaaaaacata cctgtgatag ctttaat
//
LOCUS       DADXTH010000151         1762 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.159, whole genome shotgun sequence.
ACCESSION   DADXTH010000151 DADXTH010000000
VERSION     DADXTH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.159"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(429..1001)
                     /locus_tag="IWI97_002749"
     CDS             complement(429..1001)
                     /locus_tag="IWI97_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2464195.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1017..1622)
                     /locus_tag="IWI97_002750"
     CDS             complement(1017..1622)
                     /locus_tag="IWI97_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBC2464196.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          516 a          361 c          259 g          626 t
ORIGIN      
        1 aatatcacaa taggaatgct gtttattata ttagcagcag tgctgatttt tatgatagtt
       61 atttggttct taaatgaaag atctaaagac aatcaacacc gtagggatat ggagataaaa
      121 tcaatagagt caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg
      181 tgacttacgc tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat
      241 atgatggtat cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac
      301 agttaccata cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca
      361 acatgctaat agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga
      421 cttctttttt aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa
      481 ccggttatag cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc
      541 ctcttcttcg gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt
      601 cttgcaaaca tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct
      661 acactcaaca acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga
      721 ttcaaaacct tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct
      781 ctcgtgttac gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga
      841 ccagccaaca gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa
      901 aaaatattct tcttctctac gccgttctca agcaactgtt gaatttgttc ttttgtactt
      961 tgagaagcgg ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac
     1021 aaatcatcta tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc
     1081 atatataact cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt
     1141 tccaaatcgt ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag
     1201 ttccaatcta caactttttg caattcttcc tttaaaatga gatccagcca aattctagta
     1261 cttcggccat ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata
     1321 atctcttcaa aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac
     1381 ataactggtg caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca
     1441 aaatcgtaaa tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca
     1501 acttcgattt catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt
     1561 ttttcttcaa ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc
     1621 atgcgaaatc tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat
     1681 cgcgattttg tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag
     1741 tgtctctatt ttgcacagtc ta
//
LOCUS       DADXTH010000152         1751 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.161, whole genome shotgun sequence.
ACCESSION   DADXTH010000152 DADXTH010000000
VERSION     DADXTH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.161"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            504..1634
                     /locus_tag="IWI97_002751"
     CDS             504..1634
                     /locus_tag="IWI97_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBC2464197.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          512 a          284 c          360 g          595 t
ORIGIN      
        1 cacataaaac taaaaaattt tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg atcggtcttt
      541 tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat acaaaaagtc
      601 tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc ttagctcgtt
      661 cagtcaaact acactattta cttcacccta aaatagcagg atacggttta gccttatcga
      721 ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac ggcgcacaac
      781 gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat ctttttttga
      841 ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat ctaaaaaaac
      901 cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag attgctggag
      961 cattgatgat cctttcgatt gcgtgggtca tattttgggc agcggcggtt ccatttaaaa
     1021 aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca ggcggggtat
     1081 tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat gaaaggatcg
     1141 cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg acccattcgt
     1201 tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt atcacaaaaa
     1261 aagggtatct tccagagaca gaaacagatt ttattttttc gattattaca gaagagcttg
     1321 ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg agaatctttt
     1381 gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga ttcggaactt
     1441 tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg ccaatgactg
     1501 gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt tctttaggaa
     1561 tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttggcg ctctatcgac
     1621 ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata aagtacaatc
     1681 ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa agtatctcaa
     1741 aaaatcatat t
//
LOCUS       DADXTH010000153         1665 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.162, whole genome shotgun sequence.
ACCESSION   DADXTH010000153 DADXTH010000000
VERSION     DADXTH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.162"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..506
                     /locus_tag="IWI97_002752"
     CDS             132..506
                     /locus_tag="IWI97_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBC2464198.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="IWI97_002753"
     CDS             complement(620..1249)
                     /locus_tag="IWI97_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBC2464199.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="IWI97_002754"
     tRNA            complement(1383..1455)
                     /locus_tag="IWI97_002754"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          558 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tactt
//
LOCUS       DADXTH010000154         1642 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.163, whole genome shotgun sequence.
ACCESSION   DADXTH010000154 DADXTH010000000
VERSION     DADXTH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.163"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            127..1593
                     /locus_tag="IWI97_002755"
     CDS             127..1593
                     /locus_tag="IWI97_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBC2464200.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          459 a          296 c          337 g          550 t
ORIGIN      
        1 ttgccattaa aaattacttg cttttataga aaattagttt atatttctta aataagtttt
       61 ttagttaaat ttttcggtaa agatgtataa tattttctga aaatttaaag aattggggta
      121 tagattatgg aaaaacagca agacaagggc ttcttcgggc agccgaaggg attgtcaact
      181 ttgttcttca ctgagatgtg ggaacgattt agttattacg gtatgcgtgc attacttatc
      241 tattatatgt acgatacagt tgccaacggg ggacttggat taccacgagc aacagcatta
      301 gctatcatgt cgatctacgg atctttgatc tacatgtcta gtatcgtcgg cggatgggta
      361 tctgaccgag tgttaggagc aaaacgcact gtattcttcg gtggagtttt cattatgctt
      421 ggccacatcg tcttggcttt accatttggt gtaagtacat tgtttgtttc gatggcattg
      481 attattatcg gaaccgcctt tttgaaacca aatgtttctg gcatggtcgg acatctttat
      541 tcaaagactg acttgcgaag agatgcagga ttttcgattt tttacatggg tgtcaactta
      601 ggggcattat tagcaccact aatcgttggt acaatcggtc aagaatataa ctatcattta
      661 gggttctctt tagctgctat tgggatgttc tttggattat tacaatacat catccaagga
      721 cgcaagacat tagacggagt gggtacaaca ccgccgaatc ctttgacaac tgaagaacgt
      781 aaaggattca tccgtaactt gatcatcgca cttgttgtta tcgctgcagt attcggcggg
      841 gcacaactaa ctggtcatct gacaattgac ttttttgtca atacaatcag tgtattgggg
      901 attatcttac cgttgtatta ctttatcaaa atgctgactt caaaagatgt aactgctgaa
      961 gaaaaaccaa gagtatgggc ttatatccca ttattccttg cagcaatcat tttctggtca
     1021 ttacaagaac aaggttcatc tatcttagct ttatttgcta gtgaacgtac acaatctacg
     1081 ctatttggtg taacgatcca agcaagctgg tatcaatcat tgaatccaat tttcatcttt
     1141 attctgacac cagtattcgt aacgatgtgg acgaaacttg gtgaacgtca accatctaca
     1201 gtagtgaaat tctctcttgg acttttattt gcaggattat cgtacttgct attgatgttg
     1261 ccgggtatgc tttatggtac aagtggaaaa gtaagtccat tgtggttgtt gggaagtttc
     1321 ttcatcgtgg ttattgctga attgtgtctt tcaccagttg gtttgtctgt gacaacaaaa
     1381 ttagcaccac gagcatttga atcacaaaca atcgctattt ggttcttagc tgatgcttct
     1441 tcacaagcaa tcaatgcaca aattgcacgc ttctacacac cgggaacaga atctgcttac
     1501 ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct tgctatttac tgtgaaagaa
     1561 ccaatcaaaa aactaatggg ctctattcat taatcaatga acataaaaac aggaattgct
     1621 tatttgcagc agttcctgtt tt
//
LOCUS       DADXTH010000155         1514 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.164, whole genome shotgun sequence.
ACCESSION   DADXTH010000155 DADXTH010000000
VERSION     DADXTH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.164"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1217)
                     /locus_tag="IWI97_002756"
     CDS             complement(<1..1217)
                     /locus_tag="IWI97_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2464201.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDY
                     YDLFLTNVKPIKI"
BASE COUNT          551 a          230 c          172 g          561 t
ORIGIN      
        1 aaaatcttaa tcggtttaac atttgttaaa aataaatcat aataatctaa cacataataa
       61 taatccaaaa ataattgtgc ccagttttct aatttctttg atacatccaa atccgcattt
      121 gcttgtaatt cttttttgag tttttccatt atgtgataag ttcgaggttc ttcttctttc
      181 atttcttcga tatacgaacg attaaaaaaa agatctggat taccataaaa tagataactt
      241 ctttcatgaa atgcaataaa attgaatatg agttctggat cctttatatc taaagaaaaa
      301 cggaacatgc tttgtaaaac tttctccaaa ttctcaactg ctcgataaga acaagtattt
      361 tgtcgctgat gtgcatatgc atgactattt tcatatattg catttccatc aattactcca
      421 aaagaataaa ttattcgata aaggaaacac agttcatcgc tgttaaaaga aagattgatt
      481 tgttttgacc acttcttcaa aagattaaaa tgcggatcat ctgagataac tgatttatat
      541 tcattatcta ctcgtacttt tctaaatgaa cttctttcta tattaatggc taaccaaaaa
      601 gaaaatacac gtttttgaaa aatagttaac gtaatatcat aaatcccctc aaaagcaact
      661 atatacttct ctatttcctt ttgttcgata tgaaatggcc acttatcact actttcacgt
      721 gtagaattcc aatagaaata ataaaaaaag ctacgaatgt ttgcctctga tgcattcaat
      781 tctaactgaa atggatgaaa tgttatatga taacgcctaa aatatggaat taagagcttc
      841 gcatatctga aaacagtaga tacactagta aattgctctt tagcaaaatc ttttagaaca
      901 cttctaggat atttatacat aaaatcaatc aaatgataat tataagaagc tttataaaaa
      961 tgaagataaa tattataaat aggaaaatct ttctcttttt ttatgaaaaa tcgtttatta
     1021 tcttcatttt tcagaaggat attaccatta tattgcttaa ataaatcctc tagttcttct
     1081 atatagtgta agactgactt ctccgtaaca ttaagtaaat ttctaagttc ttccattgca
     1141 caccaattgt ctttttctaa caaataatct aataaaacta cttttttgaa agatgtttta
     1201 gttaaaaaag tttgcatcat ttcacctcaa taagttactt ttgtttattt taatcaaaga
     1261 ataaacttct ctcgttcgac tataactgat cgaacaaaag aagcctttta ataaatataa
     1321 tctcacttac atacataggc tagttatttg cttctagctt atgataagta tataaacgga
     1381 tatcgatttc ataattgttt tatcttaaac atcatagctg taattgtttt tatttttaca
     1441 tgaagaatta ttataatcta gtttaatttt tttctgaaat atagaataaa aataaaaaca
     1501 tcttttcatt ttga
//
LOCUS       DADXTH010000156         1480 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.165, whole genome shotgun sequence.
ACCESSION   DADXTH010000156 DADXTH010000000
VERSION     DADXTH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.165"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(41..1381)
                     /locus_tag="IWI97_002757"
     CDS             complement(41..1381)
                     /locus_tag="IWI97_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBC2464202.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          403 a          286 c          326 g          465 t
ORIGIN      
        1 ggtgaagaaa ttcaatagaa taatcgagat actcacacgg ctagacagct atcttgtcta
       61 gccgtgttgt tgttctggct ttagtcagtt gggtagtgtt cttagttttc ttgaaatatt
      121 ttcctcgata taaaaagatt cgttctcggt agttttgaaa gtttctaaag ccataggcaa
      181 ttcgttttag tactttgata tggttgttca aacattctat agggccgttg gaatagggaa
      241 ctcgcaacgc tcgtttgatt tctgttcgat atttcttgaa ggtcgtgaac actggttgat
      301 agtgctctgg caactccttg tagtcttctt ttaggagcgc gacgaattca ggaacatctt
      361 gtcttctgat agcggaaaga aaaacctggt agacttcgta gcctcgcttt aaggagtcat
      421 cataatataa tagccgatcg actatctccg cttctgtcag atgatctcga aatgacggac
      481 gccagtagcg atgctgacca ttcaattccc acgcattctt ttgaagtagt ctccagtatt
      541 tctttaagtg cttcgaccga cgatcccctt ttttaaaggt cttcattact tgaattcggt
      601 ggttggtaaa ggcccgaccg atgtgttggc taatatgaaa gcgatcggcg acgattcggg
      661 cattcggaaa acactctttc accaatgtgc gatagggtgt atagaaatct gaaaccacta
      721 gacgtacttg ttcacggact tttctagggt agcgcaagaa atagtcgcgt aaaggattga
      781 gttgccgatt ttccacaata tcaatcagtt gatgggtctt gccgtccatc atgacaaagc
      841 tcatggctcc cgagacgttt ttcaccgatt tgaattcatc gaaacacacc acttctggta
      901 acggctggtt caaagaaacg gttttaggcc gaaggctgcg aagtattcga atgacagtgg
      961 tcggagaaat gtgcttcata cgtgcaatca aagacataga aataggctca cttaatagct
     1021 caagaatggc ttgtttgact ctgcgggcaa tggagcagcg acgatcgcta actgaatctt
     1081 ctgctaaaaa agtctttcca cagtgtttac atttgaagcg ctgtttgtta atccgcacaa
     1141 tggtcttgta gttactgaca tcattcaata agatggatac ttttttccag ccccatttaa
     1201 tgatcgtttg cttattttga tagtggcaat ggaagcattc ctctggttga taggtcaatg
     1261 tgccatcaaa aacaaaacat gtttcgtcat taatgcgctc tttgcgaaag gcatcttcat
     1321 gaaaggtaag atttaggtct agaatatcta gtaactcttt ggtaaactct gaataggaca
     1381 tcttgaaaca ctcctttgaa tggttttcgt cgactttatt ctaaaggatt tcaaggtgtc
     1441 tgtttgcgtt tattagaaaa aagaaaaagt attggcaaca
//
LOCUS       DADXTH010000157         1358 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.166, whole genome shotgun sequence.
ACCESSION   DADXTH010000157 DADXTH010000000
VERSION     DADXTH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.166"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            78..>1358
                     /locus_tag="IWI97_002758"
     CDS             78..>1358
                     /locus_tag="IWI97_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBC2464203.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          463 a          262 c          257 g          376 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat caccacaaaa taaacaactg
       61 agaggacgat acgctatatg aatgattcta tcaaaaaaat gctgagaata acagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cccttcagaa gaaaaagacg ttggtcgtcg
      181 atgctgttct ctcgcctact cctcgtgctt gtagaagttg tggttctact gtggtagatg
      241 gaaacgggaa agcaattata gtgaaaaatg gaaaaaagga aacgattgtc cgttttgaac
      301 aatacaatca tatgcctttg gttatgcgcc taaaaaagca acgctatacc tgtaaaaact
      361 gtcgcaccca ttggacggct caaagttatt ttgtccaacc cagacattca atcgcaaatc
      421 atgttagata taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga
      481 aaagctgtca ggtatctttg accaccgtta ttcgtacatt gaaagagttt aaaagctatt
      541 taccaaagca atctaagaag atcctcccca gagtattgat ggttgacgaa tttcgttcgc
      601 atgcttccat agaagataag atgagcttta tttgcgcgga tggcgaaaca ggaaaattaa
      661 tagatgtttt gcctacgcgt aaattacctc gattaacaag ctatttctta ggttgtacca
      721 atccagaaga agtagaattc ttggtgacag acatgaacgc cgcctacttc cagctcacca
      781 aacgtgttct gccaaatgcg aaagtcgtga ttgatcggtt tcatattgtc aaacacatga
      841 atcaagcgtt caatgagttg cgtatccgtg aaatgaatga acttcgtaaa gccggacaga
      901 aaagccaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca
      961 tcaaccatta tgaatacaaa acatggaaaa gtttccgagc accaaaatac ccatttctta
     1021 ctgaagcaat gatgattgat cgattgcttg agttttctcc gcccctaaag gaggcgtatc
     1081 ccttttttca tgaattagtt gaagcctttc gagacaaaga ccctgactta tttttctcct
     1141 tattggcaga acttcccgaa acgttagatg acagctttcg ggaaaagctt caaaaccttc
     1201 tgacctatga agaaggcatc accaacgcaa tgatctatcc ttattccaat ggaaaaatag
     1261 aagcgaagaa tacccacata aagacaatga aacgagtatc ctacggattt aaatcatttg
     1321 agaacatgag aattagaatc tttttgatca atcaatta
//
LOCUS       DADXTH010000158         1290 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.167, whole genome shotgun sequence.
ACCESSION   DADXTH010000158 DADXTH010000000
VERSION     DADXTH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.167"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            283..522
                     /locus_tag="IWI97_002759"
     CDS             283..522
                     /locus_tag="IWI97_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464204.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="IWI97_002760"
     CDS             complement(884..1144)
                     /locus_tag="IWI97_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464205.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          427 a          236 c          175 g          452 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta
//
LOCUS       DADXTH010000159         1286 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.168, whole genome shotgun sequence.
ACCESSION   DADXTH010000159 DADXTH010000000
VERSION     DADXTH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.168"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(15..1154)
                     /locus_tag="IWI97_002761"
     CDS             complement(15..1154)
                     /locus_tag="IWI97_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBC2464206.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          335 a          281 c          239 g          431 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tatttt
//
LOCUS       DADXTH010000160         1276 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.169, whole genome shotgun sequence.
ACCESSION   DADXTH010000160 DADXTH010000000
VERSION     DADXTH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.169"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..303
                     /locus_tag="IWI97_002762"
     CDS             <1..303
                     /locus_tag="IWI97_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2464207.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMLSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGIIGGLALARKAIRNK"
BASE COUNT          436 a          184 c          242 g          414 t
ORIGIN      
        1 ttgggtgtaa aaggccagaa tttattggct gacgattcgt tttggcagat tgaagccttt
       61 taccttgccc aatgtgcata caatacaaca ctgatgcttt cgccagatag aattattttt
      121 ggcggtggtg taatgaaaca agaacacatg aagaaaaaag ttcaggataa atttgttgag
      181 cttataaatg gatatgttga aataccacca attgctagct acattgttac gcccgaatta
      241 ggtgatagtg ctggaataat cggcggattg gcattggcta gaaaggcgat aagaaataag
      301 taattttaat gaataaatgt ttcattggta ctcatgtcaa aagtatttaa ctgagtaatc
      361 aaatgatgtt tgataaagcg aaaatgaatt caaataaaaa aggatcgagc aaactggaac
      421 atttgtccga tcctttttca cattctattt atttcgttta ttcaaccaat cggtagagac
      481 atccattgcg ctcttgtcgc gtggctttga tccatataat tgaccattac gaaatcatgg
      541 atcatcgctt tgaatgcgaa cttgtgtcac atctactttt ttgaccagat tttgaccagt
      601 ctattttaca ttctaatgta cttatatgag ttaatttttt gagtaaatgg taagtttttc
      661 cttgtttgtt ttatcttaaa ctagtgtgta atttgaaaat ctatatggaa gggtctgtgg
      721 ggtaatactt aaataaaggc taggaacctg ataacatcaa ggttcctagc ctttttataa
      781 taaaatgaag cgaaaataat ttcttttaaa gagccgagat tatcagctat ataagagtga
      841 atttgatgca aaaaacagag ccgaaacagt cttggtgttc gtagctctgc ttatatgttt
      901 cgaatttaag acaaagagtt ggcaaaagat gttaataagg aagataagat aactgtgtta
      961 tataaactag gtaactaact gtaacaagct aattttaaga gattcttatt agaagacgtt
     1021 taggaatata actaagaaaa tgattataca ggtaattgtc atttcaataa tgttttctaa
     1081 ttttcgcgtc tttgttttaa gaaaaaggcc agaatcagtt gaaaaaagtt cgtacgatag
     1141 tgaagagggg caaaatttcc tactaatatt cccactaaca agagataaaa cggccagttt
     1201 ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa tcccatagat taacaactga
     1261 ataattttta caatga
//
LOCUS       DADXTH010000161         1195 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.170, whole genome shotgun sequence.
ACCESSION   DADXTH010000161 DADXTH010000000
VERSION     DADXTH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.170"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWI97_002763"
     CDS             <1..1076
                     /locus_tag="IWI97_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBC2464208.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DADXTH010000162         1174 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.171, whole genome shotgun sequence.
ACCESSION   DADXTH010000162 DADXTH010000000
VERSION     DADXTH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.171"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..831)
                     /locus_tag="IWI97_002764"
     CDS             complement(<1..831)
                     /locus_tag="IWI97_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464209.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     K"
BASE COUNT          441 a          167 c          155 g          411 t
ORIGIN      
        1 tttagaaaaa aatgtgtatg aatacctgat tgttacttta tttattccac tttcactttt
       61 tactgtaaca aaaggacctc cttgttttag tactgtagca ttttttgaat taacagtaac
      121 cgttaatcct ttataccgga atacaggtaa aactattgtt ttgccttttg aagatttaaa
      181 cgtaaatgta atttcatctt tatttggaga aaagctacta taaataactt gaccatcttc
      241 tgtaacttga tggtttttca aaagcaattg atctttttta tagcctatag gtacataatc
      301 aaaattctca aattcttcaa gtttattttc catttgttca ttagaataag ttccttgaaa
      361 attcatttct ttcaccatgt taactactga tgaataattt aaaattgaaa tagctaatat
      421 taataatagt ataaaatttc ttctccttgt ataagaaaat ttttccaact gtggagaaat
      481 aaatagagat actgaaaaag atatgaacaa tgttgaaaat atcataaaac gccaaggagc
      541 ttgaataata tttaaaggag tattttgtaa ggcaaaccat ggaaatattt ttgtacaaag
      601 tagaatagat attataccca ttatataagt gattttagat aagattccta attttttaaa
      661 gttaaatgtt agtataatta aacaaataag caaagttaag ccaataccaa atgatagtaa
      721 ttctgaatct atacttccaa tcaaaaactc tttacccaga taaattgctc ttttttgtaa
      781 tatatccaca taaggtctat tcagatcttg atacaatgtc tgctcaatca taggacctat
      841 aaacgctagt gaaagaagta aagtaactat agcagctttt attaatttaa taattaagaa
      901 gatttctatt ttctttttcc gataaaattg aataattaaa gcaattataa tcattaccat
      961 agtcatagct acagaaagaa catgagtgta aattaataaa gtcatgctaa tcgccaacat
     1021 aacccaagat tctttttttg agtcgtaaag ctttataaac ccgagtaaaa taagaggaag
     1081 tattgacatt gcgagtaatt cccccattgc ccctcgaaga aatatttcta cactcctaaa
     1141 agaagatgtt gtatatataa tagcgaaaaa ggag
//
LOCUS       DADXTH010000163         1128 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.172, whole genome shotgun sequence.
ACCESSION   DADXTH010000163 DADXTH010000000
VERSION     DADXTH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.172"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..939
                     /locus_tag="IWI97_002765"
     rRNA            <1..939
                     /locus_tag="IWI97_002765"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1034..>1128
                     /gene="rrf"
                     /locus_tag="IWI97_002766"
     rRNA            1034..>1128
                     /gene="rrf"
                     /locus_tag="IWI97_002766"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          304 a          239 c          335 g          250 t
ORIGIN      
        1 ccgcacgaaa ggcgtaacga tttgggcact gtctcaacga gagactcggt gaaattttag
       61 tacctgtgaa gatgcaggtt acccgcgaca ggacggaaag accccatgga gctttactgt
      121 agtttgatat tgagtgtctg taccgcatgt acaggatagg taggagccgt agaaatcgga
      181 acgctagttt cgatggaggc gctggtggga tactacccct gcgttatggc cactctaacc
      241 cgcaccacta atcgtggtgg gagacagtgt cagatgggca gtttgactgg ggcggtcgcc
      301 tcctaaaagg taacggaggc gcccaaaggt tccctcagaa tggttggaaa tcattcgaag
      361 agtgtaaagg cagaagggag cttgactgcg agaccaacaa gtcgagcagg gacgaaagtc
      421 gggcttagtg atccggtggt tccgcatgga agggccatcg ctcaacggat aaaagctacc
      481 ctggggataa caggcttatc tcccccaaga gtccacatcg acggggaggt ttggcacctc
      541 gatgtcggct cgtcgcatcc tggggctgta gtcggtccca agggttgggc tgttcgccca
      601 ttaaagcggc acgcgagctg ggttcagaac gtcgtgagac agttcggtcc ctatccgtcg
      661 cgggcgttgg aaatttgaga ggagctgtcc ttagtacgag aggaccggga tggacttacc
      721 gctggtgtac cagttgttct gccaagggca ttgctgggta gctatgtagg gaagggataa
      781 acgctgaaag catctaagtg tgaagcccac ctcaagatga gatttcccat ttctttaaga
      841 aagtaagatc cctgagagat gatcaggtag ataggtcagg agtggaagta cagtgatgta
      901 tggagcggac tgatactaat cgatcgagga cttaaccaat aggttaaaag aaaagaactc
      961 ggaaaaaaac ttatgtgact tcaaatccag ttttgagtga acaaaattta ctcaataaaa
     1021 gataacacca cagtgtggtg gcgatagcga gaaggataca cctgtaacca tgccgaacac
     1081 agaagttaag cttcttagcg ccgattgtag tgaggggttg ccccttgt
//
LOCUS       DADXTH010000164         1091 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.173, whole genome shotgun sequence.
ACCESSION   DADXTH010000164 DADXTH010000000
VERSION     DADXTH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.173"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="IWI97_002767"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="IWI97_002767"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBC2464210.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DADXTH010000165         1075 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.174, whole genome shotgun sequence.
ACCESSION   DADXTH010000165 DADXTH010000000
VERSION     DADXTH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.174"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            78..>1075
                     /locus_tag="IWI97_002768"
     CDS             78..>1075
                     /locus_tag="IWI97_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBC2464211.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEA"
BASE COUNT          362 a          197 c          211 g          305 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat cacgacaaaa taaacaactg
       61 agaggacgat atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg
      181 atgctgtttt ctcacctgct ccccatacct gtagaaattg tggttctact gtggtagatg
      241 gaaacgggaa agtaattgta gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac
      301 aatacaatca catgcctttg gttatgcgct taaaaaagca gcgctatacc tgtaaaaact
      361 gtcgaaccca ttggacggct caaagttatt ttgttcaacc cagacattca atcgcgaatc
      421 atgtcagata taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga
      481 aaaattgtca ggtatctttg accaccgtta ttcgtacgtt gaaagagttt aaaagctact
      541 taccaaagca atctaagagg attctcccca gagtattgat ggttgatgaa tttcgttcgc
      601 acgcttccat agaagataaa atgagcttta tttgcgcaga tggcgaaaca ggacaattaa
      661 ttgatgtctt gcctacgcgt aaattgcctc gattaacaag ctatttctta ggctgcgcta
      721 atcctgaaga agtaaaattt ttagtcacag acatgaacgc cgcctacttc cagcttacca
      781 aacgtattct gccaaatgcg aaagtagtga ttgatcggtt tcatattatc aaacacatga
      841 atcaagcctt taatgagttg cgtatccgtg aaatgaatga acttcgtaaa gtaggacaga
      901 aaagtcaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca
      961 tcaaccatta tgaatacaaa acatggaaaa gttttcgagc accaaaatac ccatttctta
     1021 cggaagcgat gatgattgat cgattgcttg gcttttctac gtccctgaag gaggc
//
LOCUS       DADXTH010000166         1073 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.175, whole genome shotgun sequence.
ACCESSION   DADXTH010000166 DADXTH010000000
VERSION     DADXTH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.175"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1073
                     /locus_tag="IWI97_002769"
     rRNA            <1..>1073
                     /locus_tag="IWI97_002769"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DADXTH010000167         1070 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.176, whole genome shotgun sequence.
ACCESSION   DADXTH010000167 DADXTH010000000
VERSION     DADXTH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.176"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..93
                     /locus_tag="IWI97_002770"
     CDS             <1..93
                     /locus_tag="IWI97_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBC2464212.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(966..>1070)
                     /locus_tag="IWI97_002771"
     CDS             complement(966..>1070)
                     /locus_tag="IWI97_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBC2464213.1"
                     /translation="MEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          361 a          223 c          182 g          304 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggattttatt
      121 ctttatttaa aactttgcaa cagaacccac aatttcattt gccataaaat cctccttcaa
      181 gattaactac ttaacaaaat tataaagagc tttctaatag gggtcaagcc cctaattaaa
      241 gacccctaaa aacagacaaa aagaccccca aaaacagaca aaaagacccc taaaaacaga
      301 caaaaagacc cccaaaagat tctgcacacc ttgggagagt aagcatacag ccgacggtaa
      361 agaggtttaa aaagaggttt aaaaagagat tataaaagag gaggcaaaat ttcccgccta
      421 aaaaactcct gcttttttcc tcaaacaagt tcgcgaattt ttctaaactg ggaaactcgc
      481 tccctacggt cactaaaaat gtgtgatcgc tacgctctca cattatactg cgcagtcgcc
      541 taaaggctcc tttgcttaaa aacagttcat aaagaactcc tttgaaaagt tctgagcgtt
      601 tgtttaagcc gttttaagac agtcttagta taaaaccctt atcttgttct caaagcgcct
      661 tacaggcggt cagggcaagc cctaacaacc ctaaaatgtt ctataagacc tctaaaatta
      721 gatctaaggc gttttaaagt tgttttggta taattatata ccagaacgtt taaaacgcct
      781 taaaacgcaa attatagcca tatgtatttt tacggaagcc ggcacactca tttgcaaagc
      841 ttcagctttg caaattcgga ttcgctcact cgaaaattga tttgttttga ctctgatcga
      901 taggtgtatt tggttctgtt gcaaagtttt aaataaagaa taaaatccct tacggtatct
      961 atgatttaag ctgggattcc caataatacc ttgatttcag tacagaccga aaacccgaag
     1021 agagtgcctt cttttcgggt tttcttatat aatcctcgga tggcttccat
//
LOCUS       DADXTH010000168         1027 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.178, whole genome shotgun sequence.
ACCESSION   DADXTH010000168 DADXTH010000000
VERSION     DADXTH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.178"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            159..920
                     /locus_tag="IWI97_002772"
     CDS             159..920
                     /locus_tag="IWI97_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBC2464214.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
BASE COUNT          292 a          161 c          195 g          379 t
ORIGIN      
        1 tttgtttctt ttttagaatc aaatgtgatt aaatagaaaa ttttacaata atatgcacat
       61 ataaataata agatgatata atcaacaaag aaaatgtgat ttttttcact atattttatc
      121 acatgaaaat tcattaagaa agtaggaatc gaagtattat gcacacacca gaagaaatta
      181 tggaactcag tattcatctt ggaacaaaaa aaatacataa acctatctta gctaaactga
      241 ttttaggttt tatcggagga gccatgattt ctctaggtta tttggcatac gtacgagtct
      301 ctgcttctat cccagaatct atggctagct tgactagttt agttggtgcc tcggtttttc
      361 ctatcggcct gctggtcatc cttcttggcg gtggtgaatt gatcaccgga aatatgatgg
      421 ctgtggcgat tgcctttatg gataaaaaag tcacattctc acagctggtt aagaattggt
      481 cagtgattac ttttgcaaat attattggtg caattttcgt tgcctatttc tttggacact
      541 ttgttggttt aactcattca ggtgtctatc tcgaccaagt gattcatatt gctgatcata
      601 aaatcgctgt ttcttggtgg caagcattag gctccggaat aggttgtaat tggtttgtcg
      661 gtttagcgtt atggttatca tatggagcaa aagatgcggc cggcaaagta ctaacgattt
      721 ggtttccagt catgattttt gtggcaatcg gtttccagca tagtgtggcc aactgttttg
      781 tgatacctgc tgccatattt gaaggtcaag cgacttggtt agaatttctc tcgaatttta
      841 tccccgttta tattggaaat atcatcggcg gagctatttt tgtttcaggt ttttattacg
      901 ttagttataa gcatcattaa gattgaaggt aatgttcatt cgagcattac cttcaatctc
      961 tttatatact atttatattt taagaacaat agccttttat tatatatatt attcaaatta
     1021 gttatat
//
LOCUS       DADXTH010000169         1004 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.179, whole genome shotgun sequence.
ACCESSION   DADXTH010000169 DADXTH010000000
VERSION     DADXTH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.179"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(228..776)
                     /gene="aac(6')"
                     /locus_tag="IWI97_002773"
     CDS             complement(228..776)
                     /gene="aac(6')"
                     /locus_tag="IWI97_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBC2464215.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          292 a          210 c          149 g          353 t
ORIGIN      
        1 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
       61 ttcttactac tcgaaactaa gcgacttttt cacaacctct ggtacacggt gttccaaaag
      121 tagcttcttc ggaaataagt tggaatttca caaaaattca agaagtaatt ttcgtaaatt
      181 ccttcttatt tcttgaagct gattacttct gtcacaacct ctttttatta ttgagaattt
      241 ggtcgaggaa taatcgtttt tgccatccaa atatccggtt tgtcccagcc atttgcattt
      301 ggtaagacac ctacgatttt ataacctaat ttttcataga attcatacgg atgttcacga
      361 aggttctgga tggaagccac tttatcaaat gtatgctcat acaggtccgt ttgacttaac
      421 gttgttccat ggtctaaatc atccgtacct aaataaatcg tgattcctcc tctggaagct
      481 acttcttttt ctaagtaatt gactaatcga gtacctattt ggttctttcg tcgggagctt
      541 tctacaacta atggatgcaa ttcccaacct gtgataccgt attgagggat tgcaccaata
      601 aatcctacta actcatcttg gtctaccgct gctaccgcga ttcgttctgg attcatcatt
      661 tcttctactt cttctgccga gctgtctcca tattcttccg gccaagtcag tctcagtaaa
      721 tcagaaagct gatctttcaa tactggatta ttacggtcaa attcactgat tatcatacgt
      781 cttccccctt gttcttgctt attctaacag tccttttgaa aaaacgaaat gttctcgctc
      841 aaaaatcttt agctaccaat cagtatctct attctttcat tatttatctt acttataaaa
      901 taaaaaaatt atacagaaac actttattca aaataggaaa gatcaggaaa gccatcaatt
      961 ggaggaaaaa actctgtttg ttcatcatga tatagattat cttt
//
LOCUS       DADXTH010000170         1001 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.180, whole genome shotgun sequence.
ACCESSION   DADXTH010000170 DADXTH010000000
VERSION     DADXTH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.180"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1001)
                     /locus_tag="IWI97_002774"
     rRNA            complement(<1..>1001)
                     /locus_tag="IWI97_002774"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          210 a          311 c          227 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc g
//
LOCUS       DADXTH010000171         1000 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.181, whole genome shotgun sequence.
ACCESSION   DADXTH010000171 DADXTH010000000
VERSION     DADXTH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.181"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            369..1000
                     /locus_tag="IWI97_002775"
                     /pseudo
     CDS             369..1000
                     /locus_tag="IWI97_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          343 a          181 c          182 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccactact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DADXTH010000172          982 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.182, whole genome shotgun sequence.
ACCESSION   DADXTH010000172 DADXTH010000000
VERSION     DADXTH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.182"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..312
                     /locus_tag="IWI97_002776"
                     /pseudo
     CDS             1..312
                     /locus_tag="IWI97_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          364 a          125 c          166 g          327 t
ORIGIN      
        1 gaacaaataa gattattttt aaagttttta aaagttaaag ttcaaaataa ctacttcaca
       61 tgttatagtt atgcacaccc tgttttaaat agtcttcctt ttaattcttt agtgcaagga
      121 ttgattacaa atattgtttt attagtctgt acaccaaatg aaatatttgt aaaaaagata
      181 ggcagtggta tttcttcagt aagattaaaa gagaaagaat atactcaaaa gattattcat
      241 attcctaagc ctgaaattaa gcgttttgag ataaaagact attctgcttt agggctgttc
      301 tcaattggtt actggtgata aaaaataaga atactaaaac tatacgtaga atataagtaa
      361 tcggcatcaa tcctaattat gtgattgaat gccgattttt gttacgcgct atttttaatt
      421 atctgtaaga agaataattt tattttactc atattcgttt tcactttgaa gaagtaaatt
      481 gcaatttctt ataagttaag cagctatgca aaaggctatt tattctattg tgaaatataa
      541 gatgtagttg aaacaggata tatagaatca aaatacgaat cttattttga tttttctagt
      601 tttttgaaaa aagcaccctt tgatcgttgt attagtggaa aagagaaaaa ggagcatact
      661 tatcatgtca caaaactaca actaaatatc taaacaacac caatttgatg cctattgaaa
      721 aaaagtattg cgaaatgaag ccaagtctat tagaaaatga aataatagaa taaaagaaaa
      781 tgggaaaccg ctgaatgatg taaatgaagg taagtatcca cgaactaatt ttgatgaaca
      841 agatatctat tttttatttg tattggagat actagtttaa cagaattagg cagaagtcgc
      901 ccatcctcta taggtgggtg atgaatgccg ttcggtatga gggttaccgt tggtaactcg
      961 aagaccgaca actaaggcgt at
//
LOCUS       DADXTH010000173          890 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.183, whole genome shotgun sequence.
ACCESSION   DADXTH010000173 DADXTH010000000
VERSION     DADXTH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.183"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWI97_002777"
     CDS             <1..888
                     /locus_tag="IWI97_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBC2464216.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DADXTH010000174          875 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.184, whole genome shotgun sequence.
ACCESSION   DADXTH010000174 DADXTH010000000
VERSION     DADXTH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.184"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(122..520)
                     /locus_tag="IWI97_002778"
     CDS             complement(122..520)
                     /locus_tag="IWI97_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBC2464217.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(517..753)
                     /locus_tag="IWI97_002779"
     CDS             complement(517..753)
                     /locus_tag="IWI97_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBC2464218.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          282 a          148 c          125 g          320 t
ORIGIN      
        1 aaattaatcc atattttcca tatttttatg aaaaaactat tttgtatgat taaacataaa
       61 aaaatcagca tattccttaa tgacatgctg atctaattga gaaataaagt ttttaattcg
      121 ttcattttat tgcggtttgt cgtaaataac tggttatctt ggactttagt gaatcgaaat
      181 ccaatgaact agtggtaatc attatgataa aatcaacagc ttcttgacga gaaaaagcaa
      241 ctggacaatt attcaattga agaaacaaca acatggcaac aagagcagtt cgtttattgc
      301 cattatgaaa aggatgtttt ttagctaaat taattgctaa aatcgccgct ttttcataga
      361 tttcaggata gagttcttga ttgaatactg cttgtgaagg ctgtttgacg gccatatcta
      421 atgcatttgc atcttttaca ccgatcattt cgcctggcga gtgacgtaaa ataactttcg
      481 cgttgatttt gataatttct ttagtcgaaa gatacctcat aaatcaacca aatcttttaa
      541 cgcgtcttca tattgattca tgatcgaatc aactaatgaa tcaatatcag gttcttcttg
      601 tgctttgatc attcgtacag aaccatcaga ttgaaaaaca taactgattg cgtcacctgt
      661 ctgcacaccc atgaattctt ttacttctgg tgggatagtt gttacatcgc tattgccaga
      721 ttttctaatt ttatactttt tcattttttt catatttcgc caccctctat accattctca
      781 ttctatagta agtacattat atccaagtat atacttttaa tcaatgtata tacttttatt
      841 ttgctttttc ttacgaaaaa atgacaaaga aaaaa
//
LOCUS       DADXTH010000175          858 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.185, whole genome shotgun sequence.
ACCESSION   DADXTH010000175 DADXTH010000000
VERSION     DADXTH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.185"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..309
                     /locus_tag="IWI97_002780"
     CDS             <1..309
                     /locus_tag="IWI97_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2464219.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMFSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGIIGGLALARKAVRNKQP
                     "
     gene            455..652
                     /locus_tag="IWI97_002781"
     CDS             455..652
                     /locus_tag="IWI97_002781"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBC2464220.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT          287 a          117 c          154 g          300 t
ORIGIN      
        1 ttgggtgtaa aaggccagaa tttattggct gacgattcgt tttggcagat tgaagccttt
       61 taccttgccc aatgtgcata caatacaaca ctgatgtttt cgccagatag aattattttt
      121 ggcggtggtg taatgaaaca agaacacatg aagaaaaaag ttcaggataa atttgttgag
      181 cttataaatg gatatgttga aataccacca attgatagct acattattac gcccgaatta
      241 ggtgataatg ccggaataat cggcggatta gcattggcca gaaaagcggt aagaaataag
      301 caaccataat taatgaatgt ttcattgata ctcatgtcaa aagtatttaa ccgagtaatc
      361 aaatgatcaa atgaagttgg ataaagcgaa aatgaattca aataaaaagg ctctaagtca
      421 aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt ctgcgtttcc ttttttcttt
      481 ttgttgtgcg ctgtatacct tgaggatatt cactggtggc gtacacttaa atagtaatat
      541 attgtgttac tttatgtctg tggtaatagt tatagttgct taccaatgta tgttcatttt
      601 tcgaaatgtt gacttcataa tccttggctt aatgtttgaa tttttttggt gatagatttt
      661 ttcagccgta taaaataaat cgtaattcta ttatagagag ttgtgattat aaaaagatag
      721 caagaaaaaa aaagaaagtt tctatacatt ttaaatattt tattcctatt tctattgcta
      781 aaaaagtaga aattctatat ttacctttaa ctctcgcttc tcttgcaaat gtagttagca
      841 ttttaatcga taataaac
//
LOCUS       DADXTH010000176          798 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.187, whole genome shotgun sequence.
ACCESSION   DADXTH010000176 DADXTH010000000
VERSION     DADXTH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.187"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            140..736
                     /locus_tag="IWI97_002782"
     CDS             140..736
                     /locus_tag="IWI97_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBC2464221.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          277 a          149 c          135 g          237 t
ORIGIN      
        1 ttttcttatt aaaattgttt tttgaacttc gcttattttt tgtgagcata tctgtttact
       61 tactttttgt aaggtgctat acttcatttc gtaatcaatt aaaaaaatgc aaaacgcaaa
      121 tcaaaggaga aattttatta tgacaacttt tactgacact ttgaaaaacc gccgttcaat
      181 ctatcatcta ggacgtaatg tttcactttc taatgaagaa ttaacaactt tgatcaaaga
      241 agcaattaaa gaaagcccaa ctgctttcaa tgctcaatca acacgcgcag ttatcctttt
      301 cggagatgca catgaaaaac tttgggaaat cactgaagaa gcacttcgcc cattgactcc
      361 agcagaagct ttcccaaata cacaaaacaa attagctggt ttcaaaaatg gttatggtac
      421 agtattattc ttcaaagata ctgacgtggt aaaaggtcta caagaacaat ttgaattata
      481 tgcagacaac ttcccagatt ggtcagaaca atcaaacgga atcgcaacag ctaatacttg
      541 ggtagcatta gttgataaag gattgggcgc aaacttgcaa cattacaacc cagttattga
      601 cgaagcagtt gcaaaagaat ggaatatccc ttcaaactgg aaactacgtt cgcaattagt
      661 attcggttca ccagaaacac cggctgagga aaaagaatac atgaacgatg ccgatcgctt
      721 ccgcgtattt ggataatttt atttgaaata aaaaacagag gttgtgacaa acatcttgtc
      781 gcaacctctg tttttatt
//
LOCUS       DADXTH010000177          791 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.188, whole genome shotgun sequence.
ACCESSION   DADXTH010000177 DADXTH010000000
VERSION     DADXTH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.188"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            257..>791
                     /locus_tag="IWI97_002783"
     CDS             257..>791
                     /locus_tag="IWI97_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBC2464222.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPT
                     SGGQEPESRMIEPISLGF"
BASE COUNT          274 a          125 c          169 g          223 t
ORIGIN      
        1 aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac atccgcaact
       61 gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt tctatattag
      121 agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc agggtgcaat
      181 gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta tggaagtcac
      241 agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta ttcagctgca
      301 atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa gcttaaggac
      361 agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat ctgaggccgg
      421 aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta cagcggcgga
      481 agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg aatcatttat
      541 aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg gtgaaaaaaa
      601 ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac agaaaaatac
      661 attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa gggtaatatc
      721 aattcaatat cctacatcag gagggcaaga accggaaagc agaatgatag agcctatatc
      781 actggggttt t
//
LOCUS       DADXTH010000178          787 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.189, whole genome shotgun sequence.
ACCESSION   DADXTH010000178 DADXTH010000000
VERSION     DADXTH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.189"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            24..716
                     /locus_tag="IWI97_002784"
     CDS             24..716
                     /locus_tag="IWI97_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBC2464223.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttat
//
LOCUS       DADXTH010000179          750 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.190, whole genome shotgun sequence.
ACCESSION   DADXTH010000179 DADXTH010000000
VERSION     DADXTH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.190"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..717
                     /locus_tag="IWI97_002785"
     CDS             <1..717
                     /locus_tag="IWI97_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBC2464224.1"
                     /translation="LDIVENRQLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKK
                     LFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKD
                     ERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFE
                     ELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFR
                     SFYNFKLRIMIRHGNALIFN"
BASE COUNT          249 a          154 c          134 g          213 t
ORIGIN      
        1 ttggacattg tcgagaaccg acaactgcct tttttaaaac gttattttag tcatttttca
       61 cgagaaattc gtgaagcggt cgaatggatc gtgattgata tgtacgctcc ttatgtatcg
      121 ctcgttaaaa agctgtttcc aaaagcacag ctgattattg accggtttca catcgtccaa
      181 catattggtc gtacctttcg caatcatcga atcaaagaaa cgaaccaact attaaaaagc
      241 aaagaacaaa aacactatca attaggtaag caattaaaaa gatactggaa acttcttcaa
      301 aaagatgaac gcaagcttga ttatactcga cgcctttggc gacctggctt caaagctcat
      361 ttaacagaga ccgatattgt ggatcgctta ctgaaaggga gccctgcttt gcgtgtaggc
      421 tatcagttat atcaagactt tctttacgct gtaaaggaac gagactacgt atcgtttgaa
      481 gaactactta ccaataatat catgcttccc gaaggctatc agacgacctt gagaacgttc
      541 cagaaatttc tgccacaaat caaaaatgct ttacaacaaa gttattcaaa tggcccactt
      601 gaatgtctga acaaccatat caaagtgtta aaacgaaatg catatggctt tcgtagcttt
      661 tataatttca aattgcgcat catgattcgt catggcaacg cattgatttt caactaaaaa
      721 agatcaagag gaactgatcc acttgatctc
//
LOCUS       DADXTH010000180          741 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.191, whole genome shotgun sequence.
ACCESSION   DADXTH010000180 DADXTH010000000
VERSION     DADXTH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.191"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..703
                     /locus_tag="IWI97_002786"
     CDS             <1..703
                     /locus_tag="IWI97_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBC2464225.1"
                     /translation="RRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQ
                     LIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNY
                     EKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRD
                     ISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNF
                     KLRIMIRHGKTFLTK"
BASE COUNT          235 a          151 c          138 g          217 t
ORIGIN      
        1 tcgacgcttg ccttatttag aacgctattt ttctcggttt tcattagcga ctcgtgaagc
       61 cgttcaattg attgttattg atatgtatgc gccttatgtt tcgttggtga aaaagctttt
      121 cccgaatgct cagttgatca tcgatcgttt ccacatcgtc caacatattg gcagaacttt
      181 cttgaatcat cgagtgaaag aaaccacggt acgacttaaa ggaaattccc actctcaacg
      241 agggttaggg aaaaaattga agcgctattg gaaactccta caaaaagaag aagcaaagtt
      301 aaactatgaa aaacgggttt ggcgtgccag ttttaaagat tatttgactg aaagtgagat
      361 tgccgatcga ctgttagctg cttgtcccaa cttacgccaa ggctatcagt tataccaaga
      421 cattctttat gcggtaaaga aaagagatca gtcactattt gaggattgtt taactagaga
      481 cattagcggt ctgccagaaa cctatacaac cactttacga acgtttaaaa agtttctgcc
      541 acagatccaa aatgcgcttc actacagcta ttcaaacgga ccattggaat gcttaaacaa
      601 ccacatcaaa gtattaaaac gcaatgcgta cggctttcgc aacttttaca atttcaaact
      661 acgaattatg attcgtcatg gcaaaacatt tttaaccaaa taaacagaac agaaagaggt
      721 tttcttcttt ctgttctgtc c
//
LOCUS       DADXTH010000181          722 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.192, whole genome shotgun sequence.
ACCESSION   DADXTH010000181 DADXTH010000000
VERSION     DADXTH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.192"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            238..>722
                     /locus_tag="IWI97_002787"
     CDS             238..>722
                     /locus_tag="IWI97_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBC2464226.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     F"
BASE COUNT          266 a          123 c          113 g          220 t
ORIGIN      
        1 cttaatttta caattcgcgt tttataactt tacgcaagat cataattttg tctgtgtaga
       61 tatttcgtta tatcactaaa gataataaga tttaatgacc tcattaatat tgtttgctag
      121 gtccaaactc gagagtgtaa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
      181 gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga cttccctatg
      241 aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt aaatgaatta
      301 ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt aacggctttt
      361 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
      421 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
      481 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
      541 accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc
      601 gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac taaagttctg
      661 actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt cgctattttt
      721 at
//
LOCUS       DADXTH010000182          696 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.193, whole genome shotgun sequence.
ACCESSION   DADXTH010000182 DADXTH010000000
VERSION     DADXTH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.193"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(53..628)
                     /locus_tag="IWI97_002788"
     CDS             complement(53..628)
                     /locus_tag="IWI97_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2464227.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          125 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaacg
//
LOCUS       DADXTH010000183          682 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.194, whole genome shotgun sequence.
ACCESSION   DADXTH010000183 DADXTH010000000
VERSION     DADXTH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.194"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /locus_tag="IWI97_002789"
     CDS             <1..672
                     /locus_tag="IWI97_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2464228.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          254 a           98 c          124 g          206 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc at
//
LOCUS       DADXTH010000184          676 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.195, whole genome shotgun sequence.
ACCESSION   DADXTH010000184 DADXTH010000000
VERSION     DADXTH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.195"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>676
                     /locus_tag="IWI97_002790"
     CDS             <1..>676
                     /locus_tag="IWI97_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2464229.1"
                     /translation="KSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDV
                     IVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHERE
                     KDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQW
                     FESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRD
                     GLLKSMD"
BASE COUNT          229 a          115 c          136 g          196 t
ORIGIN      
        1 aaatcagcct tagaatatta tcaacagtat aagaaaaaca aatcaaactg tggtagacgt
       61 ccgcttgttt tacccgagga acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg
      121 acacccgatg tgattgttgg tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt
      181 tatcgtatgt tcaaaaaggg gctatttgat tcttctgact taccgatgaa aggcaagcgt
      241 aaaccgaatg gacatcaaga aagacgtgga aaacaaactt tccgccgctc tattcatgaa
      301 cgtgaaaagg attatagcca attctcaaat gagtttggtc accttgaagg tgacactatc
      361 gtaggtctga aacataaaag tgctgtaatt accttagttg aacgattatc aaaagttatc
      421 atcacattga aaccgtgtgg tagacaagcg attgatattg aaaaaaaatt aaatcaatgg
      481 tttgaatctg taccgaaaaa cctattcaaa tccatcactt ttgattgtgg aaaggaattt
      541 tcaaattgga aacagatcag taatgtcaat gatattgcca tttatttcgc tgatccagga
      601 acgccgtctc aaagaggcct aaacgagaat tctaacggat tgttacgtag agatggttta
      661 ttgaaatcta tggatt
//
LOCUS       DADXTH010000185          665 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.196, whole genome shotgun sequence.
ACCESSION   DADXTH010000185 DADXTH010000000
VERSION     DADXTH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.196"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>665)
                     /locus_tag="IWI97_002791"
     rRNA            complement(<1..>665)
                     /locus_tag="IWI97_002791"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          132 a          203 c          137 g          193 t
ORIGIN      
        1 tatagttacg gccgccgttt actggggctt caattcgtac cttcgcttgc gctaagcact
       61 cctcttaacc ttccagcacc gggcaggcgt cagcccctat acttcatctt acgattttgc
      121 agagacctgt gtttttgata aacagtcgct tgggcctatt cactgcggct gatcgtatga
      181 tcagcacccc ttctcccgaa gttacggggt cattttgccg agttccttaa cgagagttct
      241 ctcgctcacc ttaggattct ctcctcgact acctgtgtcg gtttgcggta cgggccgctg
      301 ttttctcact agaagctttt ctcggcagtg tgacatcagg aacttcggta ctattatttc
      361 cctccccatc acagcttgtc cttaagaaaa gaagcatttg actcctcttc agacttactg
      421 cttggacaga catttccgat cgtctgcatt ccttagcctc ctgcgtccct ccattgctca
      481 aacaaaaaca acgggtacag gaatatcaac ctgttatcca tcgcctacgc ctgtcggcct
      541 cggcttaggt cccgactaac cctgggcgga cgagccttcc ccaggaaacc ttagtcattc
      601 ggtggacagg attctcacct gtctttcgct actcataccg gcattctcac ttctaagcgc
      661 tccag
//
LOCUS       DADXTH010000186          659 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.197, whole genome shotgun sequence.
ACCESSION   DADXTH010000186 DADXTH010000000
VERSION     DADXTH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.197"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..429)
                     /locus_tag="IWI97_002792"
     CDS             complement(<1..429)
                     /locus_tag="IWI97_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBC2464230.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          228 a           85 c           81 g          265 t
ORIGIN      
        1 gttattaact tgattaaaat cagagataat atcatttaat tcagtttgaa gggttgtaac
       61 gattaaatgg aaattttctt catctgtttt attatatggg gatgttttcg ttatatataa
      121 caacttttct ttttcaattt tcattaataa ctctgtcgaa gtctgtactt tatcttgata
      181 attaaatgca gacataccac tttgtataat tgtaattatt gctgaagcaa ttgaaacaat
      241 caatagtaaa gacctgtgtt cggaagcctg atcaattaat attgggatac tagctgataa
      301 gactattttt ataatatttc cgataccaat tactctatta taaaatcgaa tttctttttt
      361 taatctatcg attgtagaat caatagattc gataaacgct ttttgatcca tcattttctc
      421 tccaatcatt ttattaaaat cattaacaaa aatattataa taggatttaa gtaagttcta
      481 actaatcaca tataaaaata aaaaaacatt tgtattttat caaagtttta ttatacttgt
      541 caatttgtgt acatcctaaa acataagcta tttgtttact tctgtagaat atatactttg
      601 ttgttagata agttgcacat gtttatagaa tctaaataat acccgtggtg ctagttaat
//
LOCUS       DADXTH010000187          647 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.198, whole genome shotgun sequence.
ACCESSION   DADXTH010000187 DADXTH010000000
VERSION     DADXTH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.198"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            94..>647
                     /locus_tag="IWI97_002793"
     CDS             94..>647
                     /locus_tag="IWI97_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HBC2464231.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIM"
BASE COUNT          204 a          131 c          117 g          195 t
ORIGIN      
        1 cagcatcttt tttattttcc aaaacatata gaaaagacac cttgaaatcc tttagaatta
       61 atttggcgaa ccaacccaaa aggaggaatc aagatgtctt acactcacct tatcaaagaa
      121 acattagata ttctagactt atcagttact tttaacgaaa attgtctcac aaaagaaaaa
      181 tacaaagggc aaatctgtca tatttatcgt ggaaacttga tttatacagc ccaagaatgc
      241 atccactgta aacaccagat tgcttcagat attgtgcgtt ggggtacaac aaccgtccgg
      301 ttgttaatga acgatgtttc tgaataccgt acctacctcg aattgaaaaa gcaacgcttc
      361 aaatgcaaag cttgtcagcg gacctttgtc gcagatactt ctgttgcgaa aaaacattgt
      421 ttcatcagtg agaaagtccg ctggtctgtc gtgactcgtc tgaaaaaaaa tacctcaatg
      481 acagagatcg ccgctcagaa aaatctatcg gtctcttctg tttaccgtat tatgaagcga
      541 ttttatcgtc cgttgaatcc ttttcggaca ccgttaccta aggtactttg ttttgatgaa
      601 ttcaaatcag ttcggggtgt ttcaggtgct atgagtttca tcatgat
//
LOCUS       DADXTH010000188          646 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.199, whole genome shotgun sequence.
ACCESSION   DADXTH010000188 DADXTH010000000
VERSION     DADXTH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.199"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            90..>646
                     /locus_tag="IWI97_002794"
     CDS             90..>646
                     /locus_tag="IWI97_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBC2464232.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMM"
BASE COUNT          201 a          124 c          121 g          200 t
ORIGIN      
        1 caacatcttt tttgtataaa aaaatataga aaagacaccc tgaaatcctt tagaattaat
       61 ttggctacca attcatagga ggattcaaga tgtctcatac tcagcttatc aaagataccc
      121 taaatattct agacctaaat atctatttcg aggaaaattg tttaactaaa gaaaaataca
      181 aagggcaaat ttgcttgatt tacaaaggaa ctcttctgta caagccagaa gagtgttccc
      241 attgtctttg tgtcgtcccc tctaggatca ttcgttgggg gacgacaacc gttcggttgt
      301 tactgaatga tgtgtctgag tatcgcactt atctggagct gaagaaacaa cgatttaaat
      361 gtaagtcttg ccaaagaaca ttcgtagcgg atacctctgt tgctgaaaaa cactgtttca
      421 ttagtcaaaa agttcgttgg tctgtcattg ctcgcttaaa agagaatact tccatgacgg
      481 aaatcgctcg tcagaaaaat atttccactt cttcggtcta tcgtgtcatg aagcgattct
      541 accgcccatt gaatcctttc aaacagactt tgccaaaggt cttatgcttt gatgaattca
      601 aatcggttcg aagtgttgcc agtgcaatga gttttatcat gatgga
//
LOCUS       DADXTH010000189          641 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.200, whole genome shotgun sequence.
ACCESSION   DADXTH010000189 DADXTH010000000
VERSION     DADXTH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.200"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..283
                     /locus_tag="IWI97_002795"
     CDS             <1..283
                     /locus_tag="IWI97_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2464233.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          219 a           89 c          119 g          214 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt a
//
LOCUS       DADXTH010000190          617 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.201, whole genome shotgun sequence.
ACCESSION   DADXTH010000190 DADXTH010000000
VERSION     DADXTH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.201"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          218 a          116 c           93 g          190 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatatagga tagatacaga ttgatttgtt tagtgtatga ttgcatatca tggacatata
      181 tcgctaagta tcgcgcccaa aacataggtt tcgagtttca tgaatatcct aacaggtaga
      241 agtgctatga tgatattcga taagcatcct aatatgaaat ataagtttag aactgctatt
      301 ttggatagtt cttcctccta ttcaaataaa taattgttct acataagaat ttcacaatcc
      361 tctctaccct cctctactac caagtgaaaa tccatttcaa ctaataccaa aaacctacat
      421 tgggattcca aaggattttt gagttcaaaa aaacatgaaa tgcctctcag aaatccgtcc
      481 attggattca acatatactt atgcacaaaa agcagtgaat aaatttaaaa aactggtttt
      541 tctatatatc tcaatttgaa aaaagtagaa tatagaggaa ctttcttacc ataagaattc
      601 acatatttaa tgtgatg
//
LOCUS       DADXTH010000191          605 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.202, whole genome shotgun sequence.
ACCESSION   DADXTH010000191 DADXTH010000000
VERSION     DADXTH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.202"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          201 a          110 c           63 g          231 t
ORIGIN      
        1 cctttaaacg aggtacttgc ttaaatactt ctggttgtac ttctaaaaat aaattatcgc
       61 tcattttcac gaccaccatc gacttccaca gttgaaattg acattttatt taacacacct
      121 attccccctt aactataact caattctata taaaaaatat tctattctca aataattgaa
      181 cttttcaaat cgccaattta cttcggcttt atcagagctc attcatatca agagcgatta
      241 acgctgtaaa aattaaaccg aagatctgat aaattttctc tattttctac aatatttaaa
      301 acgcttcctt agtctattat atatctattt ctcttaggag tatgtttaga tgtttgaata
      361 atagttgccc aactttaaag gttattctta taatatttta cttcattttc caatagaaaa
      421 ttcaaaggat tactggtttt tctaaaatag tttagtttaa atacataagt atcctccgac
      481 actgtattag ttcataatca tctaagtcaa gtccagactc ctgtgtaaaa tgctatacaa
      541 tgtttttaca atttctactt atcaaaattg atgtattttc ttgaagaata aatccattca
      601 tcatg
//
LOCUS       DADXTH010000192          544 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.203, whole genome shotgun sequence.
ACCESSION   DADXTH010000192 DADXTH010000000
VERSION     DADXTH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.203"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            73..189
                     /locus_tag="IWI97_002796"
                     /pseudo
     CDS             73..189
                     /locus_tag="IWI97_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            202..>544
                     /locus_tag="IWI97_002797"
     CDS             202..>544
                     /locus_tag="IWI97_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2464234.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKND"
BASE COUNT          208 a           53 c           97 g          186 t
ORIGIN      
        1 tcaaattatc ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaataaacg
       61 aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa
      121 caggatgaac gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat taatatacct
      181 aaaaaaaatg atttagagtt tatggaagaa gttttaattg atgataatat ggtttttgat
      241 attgataatt taaaaggatt tcttaatgat accagttcat ttgggtttat agctaaagaa
      301 aataataaaa ttataggatt tgcatattgc tatacacttt taagacctga tggaaaaaca
      361 atgttttatt tacactcaat aggaatgtta cctaactatc aagacaaagg ttatggttca
      421 aaattattat cttttattaa ggaatattct aaagagattg gttgttctga aatgttttta
      481 ataactgata aaggtaatcc tagagcttgc catgtatatg aaaaattagg tggtaaaaat
      541 gatt
//
LOCUS       DADXTH010000193          541 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.204, whole genome shotgun sequence.
ACCESSION   DADXTH010000193 DADXTH010000000
VERSION     DADXTH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.204"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            145..402
                     /locus_tag="IWI97_002798"
     CDS             145..402
                     /locus_tag="IWI97_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464235.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          231 a           66 c           75 g          169 t
ORIGIN      
        1 atttttctgt ccacgttcat tttactccaa agttttcctc atttcaaaag aaaatcacag
       61 aatacacgaa gaagcgtttc tatttgtata aactatatca aagttctata cttaaattat
      121 caaataaaaa ggacgtgaag ataaatgtca aatttagaaa acaaagaaga aaaagttgta
      181 aacaaaattg tttctgttgt caataaactg gataaagagt tagacgaact tgatacgtta
      241 tctgaaaatc cagaaaaaaa acataaccta aaaaaatggt tagttgaacg aaaggctatt
      301 catgaaatta aaaaagttct tcatgaagct gataaatatg aaaaatatga tgaaaaagaa
      361 cttgataaag aatttaaaga aatcaatgac ttattactat aattgattta tacgactcat
      421 ttttgagtcg tcagactgca aacaacactc taaatttagg agtttgtcta cagtctgaat
      481 aaattttaaa tttattgtcc ataatgttat tatgaattta tagcattaag aaaagagata
      541 a
//
LOCUS       DADXTH010000194          539 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.205, whole genome shotgun sequence.
ACCESSION   DADXTH010000194 DADXTH010000000
VERSION     DADXTH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.205"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>539
                     /locus_tag="IWI97_002799"
     CDS             <1..>539
                     /locus_tag="IWI97_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBC2464236.1"
                     /translation="GHFSPTVFERYQRNEKALMASMLEMYVSGVSTRKVSKIVEELCG
                     KSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVREENRVLSKSCHIA
                     IGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKS
                     FTNVSWQRCQVHFLRNIFT"
BASE COUNT          183 a          104 c          104 g          148 t
ORIGIN      
        1 tggccatttt tcacccacag tgtttgaacg ttatcaacga aacgaaaaag ccctcatggc
       61 ttcaatgttg gaaatgtatg tatcaggcgt ttcaactcgt aaagtatcaa aaattgtgga
      121 agaactttgt ggtaaatccg tctctaagtc cttcgtttct agcttaacag aacagctaga
      181 acctatggtt aacgagtggc agaatcgttt attatcagaa aaaaattatc cttacttaat
      241 gaccgatgta ctctatataa aagtacgaga agaaaatcga gtactctcaa aaagctgtca
      301 tatagcgatt ggaataacca aagatggcga ccgtgaaatt atcggcttca tgattcaaag
      361 tggcgaaagc gaagagacct ggacaacatt ttttgaatac ctaaaagaac gcggtttaca
      421 aggtacggaa ctcgttattt ctgatgcgca caaaggatta gtctctgcca ttagaaaatc
      481 cttcaccaac gtaagttggc aaagatgcca agttcacttc ctaagaaata tctttacca
//
LOCUS       DADXTH010000195          528 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.206, whole genome shotgun sequence.
ACCESSION   DADXTH010000195 DADXTH010000000
VERSION     DADXTH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.206"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWI97_002800"
     tRNA            complement(5..90)
                     /locus_tag="IWI97_002800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWI97_002801"
     tRNA            complement(107..178)
                     /locus_tag="IWI97_002801"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWI97_002802"
     tRNA            complement(190..262)
                     /locus_tag="IWI97_002802"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWI97_002803"
     tRNA            complement(280..361)
                     /locus_tag="IWI97_002803"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWI97_002804"
     tRNA            complement(369..441)
                     /locus_tag="IWI97_002804"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWI97_002805"
     tRNA            complement(445..517)
                     /locus_tag="IWI97_002805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DADXTH010000196          520 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.207, whole genome shotgun sequence.
ACCESSION   DADXTH010000196 DADXTH010000000
VERSION     DADXTH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.207"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          176 a           98 c           59 g          187 t
ORIGIN      
        1 tataacacag ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc
       61 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
      121 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
      181 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
      241 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
      301 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
      361 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
      421 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
      481 atacactact attcagccca ttcaacgacc aatgaatttt
//
LOCUS       DADXTH010000197          510 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.208, whole genome shotgun sequence.
ACCESSION   DADXTH010000197 DADXTH010000000
VERSION     DADXTH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.208"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..434)
                     /locus_tag="IWI97_002806"
     CDS             complement(<1..434)
                     /locus_tag="IWI97_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2464237.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT          146 a           85 c           80 g          199 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc
//
LOCUS       DADXTH010000198          500 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.209, whole genome shotgun sequence.
ACCESSION   DADXTH010000198 DADXTH010000000
VERSION     DADXTH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.209"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            63..338
                     /locus_tag="IWI97_002807"
     CDS             63..338
                     /locus_tag="IWI97_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464238.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            complement(367..>500)
                     /locus_tag="IWI97_002808"
     CDS             complement(367..>500)
                     /locus_tag="IWI97_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2464239.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          202 a           71 c           76 g          151 t
ORIGIN      
        1 gttttcatat aaccaaaggc gctatgattt ccatatgaaa aaaataaagt caggtgaaaa
       61 aaatgaaact tgaggataaa aaagtcattt tgaagaaaat caaacgacta ccagaccaaa
      121 tagatggcta tacgatctgt tctttcaaaa aagacaggag tataaaagct tatacgcaaa
      181 acacagaaaa aggaataatc tatttcctac atgaagaagg ttacgatacc caaaccttta
      241 tgtttactga aaaaaaagaa tttcataaac agatgaaaaa actgattgaa aaagaatttc
      301 caagaagtca taaactctat gtgaatcagc aattttaact tctaactgtt ctttatttat
      361 gatttgtcaa attaagctag aataaaatgc ttcttgtaca tagctcaaaa atatttcgat
      421 tggtgttctg taattcaatg attttcttgg aatatgatta cgttgattgc tgacactgga
      481 aataaatgtc tgattcactt
//
LOCUS       DADXTH010000199          488 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.210, whole genome shotgun sequence.
ACCESSION   DADXTH010000199 DADXTH010000000
VERSION     DADXTH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.210"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          187 a           65 c           66 g          170 t
ORIGIN      
        1 aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta gagaaataca
       61 aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa atccccaata
      121 cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt acactctttt
      181 tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag catgttcctt
      241 caaataagtt ttttaattat aaactctaga gaaattaatt catcctaaca taaaataatt
      301 gttcactcaa aagtttttct agaacaatta aaacagcagg cttcacattc gctatacatt
      361 tgtaagaagt aataattcct gtactaaaag gcataattgc tttaattatt ttttgacttc
      421 tcttcaaaca caagaagtag ttgtgtaata attgcagaaa taaggattcc aataatagat
      481 ataaaatt
//
LOCUS       DADXTH010000200          479 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.211, whole genome shotgun sequence.
ACCESSION   DADXTH010000200 DADXTH010000000
VERSION     DADXTH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.211"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>479
                     /locus_tag="IWI97_002809"
     CDS             <1..>479
                     /locus_tag="IWI97_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464240.1"
                     /translation="IFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKW
                     PEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDASEGIKNKEDL
                     PKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG
                     "
BASE COUNT          192 a           74 c          113 g          100 t
ORIGIN      
        1 atcttcaccg caggggaagg aacaagtatt gaaagtggaa caacggtctt tgcagtgaaa
       61 gacggcgtaa gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca
      121 gatgcgaaat ggccagaaga agcaactcaa ccaattacag cagatgatac agaatttgta
      181 tcaagtgcaa caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa
      241 aaagtgacta cagaattgaa taaggaacca gacgcatctg agggaattaa gaacaaagaa
      301 gatttaccaa aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga
      361 aataaaaaag ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta
      421 gatgttacag tgacagacaa tcgttctgac gccgataaat atgagccaac agtagaagg
//
LOCUS       DADXTH010000201          466 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.212, whole genome shotgun sequence.
ACCESSION   DADXTH010000201 DADXTH010000000
VERSION     DADXTH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.212"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          164 a           52 c           52 g          198 t
ORIGIN      
        1 tgaaacacta cttttgtaat ataaaaaata ttttattaat aataaaatta atcacatttt
       61 atatagtgac taatctagtg ttttaaaaaa ataaatgata ctatatgtaa tataaaaaaa
      121 catgaggtgg tatatgaatt tgaaaaaaat tttttctgta aaaatattta tagtttatgc
      181 atttgttagt ttattatatt tatttccagc atttaaaaac atggaaatat ttggctttta
      241 tgatacggca tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat
      301 taattttgaa acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac
      361 tctatatcct ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt
      421 tctttatatt gttacgctca ttactctatt tatatgtcat tatgta
//
LOCUS       DADXTH010000202          460 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.213, whole genome shotgun sequence.
ACCESSION   DADXTH010000202 DADXTH010000000
VERSION     DADXTH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.213"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IWI97_002810"
     tRNA            complement(20..91)
                     /locus_tag="IWI97_002810"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IWI97_002811"
     tRNA            complement(113..200)
                     /locus_tag="IWI97_002811"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IWI97_002812"
     tRNA            complement(225..298)
                     /locus_tag="IWI97_002812"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IWI97_002813"
     tRNA            complement(312..382)
                     /locus_tag="IWI97_002813"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IWI97_002814"
     tRNA            complement(384..459)
                     /locus_tag="IWI97_002814"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DADXTH010000203          459 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.214, whole genome shotgun sequence.
ACCESSION   DADXTH010000203 DADXTH010000000
VERSION     DADXTH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.214"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          205 a           58 c           38 g          158 t
ORIGIN      
        1 actaccatta ctagtaatat atactacctt atttcagtag tatatattat attattctta
       61 taagacaagg ctttaggcta gaaaacaata gattatatac tactaaaaaa tacaataagt
      121 actaccaatg tcagatatat ataataatta tgtactacca ttaaaattaa cttatttaat
      181 aagatactac tgtgttaatc tgaaaaaaat tactttaaag tagttacata ctacctgtta
      241 atttagatac taccattaat acttaaatta gatactacct atatttatat taagtactac
      301 cgttctaaga ataagataat aaaaaaatac taccaaattg caattaaata ctacttaaaa
      361 tgtaaaaaat aagaactacc caaattaggt agttcttaat aagagaatat cagataaatt
      421 tgtacaagtc ataaaaaaat taatcttaaa tttttaaat
//
LOCUS       DADXTH010000204          440 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.215, whole genome shotgun sequence.
ACCESSION   DADXTH010000204 DADXTH010000000
VERSION     DADXTH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.215"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..>440
                     /locus_tag="IWI97_002815"
     CDS             11..>440
                     /locus_tag="IWI97_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBC2464241.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DADXTH010000205          415 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.216, whole genome shotgun sequence.
ACCESSION   DADXTH010000205 DADXTH010000000
VERSION     DADXTH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.216"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          143 a           71 c           65 g          136 t
ORIGIN      
        1 gaaagacatt tgggttaaat tgtgagataa taataaagaa gagtttaaag cgcaccccaa
       61 accacttccc cataagtgtg ttacgcttta aactctttta tatttgaagc cattaaaaag
      121 cataccatat ttttgaaaaa aagtgagaaa aaaggcttac aattggagtg gtagttaatt
      181 agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc atcatattac
      241 acaatcttaa tactaactta aaaaatatct cctttcacaa gtatggtgat aaaattcgtt
      301 ccgggctacc tttttaggta gcctacttta atctttatac ctttctggat caacgaaagt
      361 atactttata tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtc
//
LOCUS       DADXTH010000206          414 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.217, whole genome shotgun sequence.
ACCESSION   DADXTH010000206 DADXTH010000000
VERSION     DADXTH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.217"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          151 a           58 c           77 g          128 t
ORIGIN      
        1 aattatgtat atttttgaaa caaaaatttt tcattgcaat aattgcaaca aagaaatttc
       61 atcgggggaa gaggtttgga caaaaatgga aatttccgcc aaaagtaact gctcctcaac
      121 tgaaatcaat taaagaacta gaatttgaga atgcactggt aatttgtatg acatgtactg
      181 atgaagtatt tcgaaaacat ttttaaaggt tgagatgtat atagatttaa ccaatcattt
      241 atctctctcg gaaaaggaat attaactaac cagcgggtta tcaaaaatag gtaggaaaac
      301 tgtacttaat ggcttatttc gcgctgatta tgagaatatc agtctgtttg attaattttt
      361 agggttcaaa atacagagac aaactccagg tcgggtttcg ccacaagcga atta
//
LOCUS       DADXTH010000207          392 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.218, whole genome shotgun sequence.
ACCESSION   DADXTH010000207 DADXTH010000000
VERSION     DADXTH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.218"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IWI97_002816"
     CDS             <1..382
                     /locus_tag="IWI97_002816"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBC2464242.1"
                     /translation="KRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWL
                     KTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNR
                     NRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          155 a           75 c           68 g           94 t
ORIGIN      
        1 aaaacgaatg actgtggatt gtgtcattca aacattgaat aaagcaaaaa tacattatca
       61 cattccagaa ggaatgattc tacacactga cttgggcagc caatacacag caagagaagt
      121 ggaacaatgg cttaaaacca acaaaataag gcattcttat agtagaaaag gaacacctta
      181 tgataatgct ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag tctacacgac
      241 tagctactca gattttgaag aagcaaatcg agcgctattt agctacattg aaggatttta
      301 taaccgaaat cgaatccata gctcgattca ctatctaacc ccacaggaat ttgaagaatt
      361 agcaaaagaa aaaatggctt aaccgtctct ac
//
LOCUS       DADXTH010000208          381 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.219, whole genome shotgun sequence.
ACCESSION   DADXTH010000208 DADXTH010000000
VERSION     DADXTH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.219"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..94)
                     /locus_tag="IWI97_002817"
     tRNA            complement(21..94)
                     /locus_tag="IWI97_002817"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IWI97_002818"
     tRNA            complement(108..181)
                     /locus_tag="IWI97_002818"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IWI97_002819"
     tRNA            complement(209..281)
                     /locus_tag="IWI97_002819"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IWI97_002820"
     tRNA            complement(301..374)
                     /locus_tag="IWI97_002820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DADXTH010000209          375 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.220, whole genome shotgun sequence.
ACCESSION   DADXTH010000209 DADXTH010000000
VERSION     DADXTH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.220"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..375
                     /locus_tag="IWI97_002821"
     CDS             <1..375
                     /locus_tag="IWI97_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2464243.1"
                     /translation="ICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQD
                     LLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQK
                     FLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          143 a           69 c           57 g          106 t
ORIGIN      
        1 atctgtgatg actttaaggc tgtttatcaa gctaactcaa aagaagaagc gaataccttc
       61 ttatccggca tgattgagaa atggaagaaa aactatccta aagtgacgca gtcactcata
      121 gaaaaccaag acttattaac tttttatgat tttccaccta gcattcgtag aaccatttac
      181 tcaaccaatc taatcgagtc tttcaataag caaattaaaa gatacagccg tagaaaagag
      241 cagtttcaaa atgaagaatc actagaacgc tttctagtca gcatttttga tacatacaat
      301 caaaaatttc taaacagaag ccataaaggt tttcaacagg taaccgatac attagtttca
      361 atgtttactg agtaa
//
LOCUS       DADXTH010000210          375 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.221, whole genome shotgun sequence.
ACCESSION   DADXTH010000210 DADXTH010000000
VERSION     DADXTH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.221"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWI97_002822"
     CDS             complement(<1..132)
                     /locus_tag="IWI97_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBC2464244.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DADXTH010000211          365 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.222, whole genome shotgun sequence.
ACCESSION   DADXTH010000211 DADXTH010000000
VERSION     DADXTH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.222"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>365
                     /locus_tag="IWI97_002823"
     CDS             <1..>365
                     /locus_tag="IWI97_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2464245.1"
                     /translation="KTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV
                     IIGRKERPVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRP
                     DNYPDFNSEFGHLEGDTIVG"
BASE COUNT          127 a           63 c           77 g           98 t
ORIGIN      
        1 aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa
       61 gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac tcttggttgg
      121 acgcctgacg tcattatcgg gcgaaaagaa aggcctgttt catgcggtat gagaacactt
      181 tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgaa aggtaaaaga
      241 aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc aatccatgat
      301 cgacctgata attatcctga tttcaattct gagtttggtc accttgaagg tgatacgatc
      361 gttgg
//
LOCUS       DADXTH010000212          344 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.223, whole genome shotgun sequence.
ACCESSION   DADXTH010000212 DADXTH010000000
VERSION     DADXTH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.223"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          138 a           44 c           44 g          118 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttt
//
LOCUS       DADXTH010000213          323 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.224, whole genome shotgun sequence.
ACCESSION   DADXTH010000213 DADXTH010000000
VERSION     DADXTH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.224"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="IWI97_002824"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="IWI97_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBC2464246.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DADXTH010000214          295 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.225, whole genome shotgun sequence.
ACCESSION   DADXTH010000214 DADXTH010000000
VERSION     DADXTH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.225"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(72..145)
                     /locus_tag="IWI97_002825"
     tRNA            complement(72..145)
                     /locus_tag="IWI97_002825"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Ile,seq:cat)
BASE COUNT           96 a           56 c           62 g           81 t
ORIGIN      
        1 aagaaaagct ttagcttgta taatactctt caaaaaattc taacacagaa cgattcaatt
       61 ggctacatta atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg
      121 ctctgaccaa ctgagctaag ggtagtggtt gttgccacat aaagccataa acaatcaacc
      181 agtagaatgc gtggcaacaa acctgttatc gcatatcttg gagtgtgact atttatgagt
      241 gatagtgaag atatgcgtct ccatatatgt atggttgaga cagataaaca tgtat
//
LOCUS       DADXTH010000215          285 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.226, whole genome shotgun sequence.
ACCESSION   DADXTH010000215 DADXTH010000000
VERSION     DADXTH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.226"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>285
                     /locus_tag="IWI97_002826"
     CDS             <1..>285
                     /locus_tag="IWI97_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2464247.1"
                     /translation="PLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGF
                     PITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGIT"
BASE COUNT          104 a           53 c           47 g           81 t
ORIGIN      
        1 tccattatgt aaaccagtta gaaatagaca agcaaaacta ttgaatcaaa tagccaaagt
       61 tggctataac tcaacaaaaa aatcttattt ttatggtctt aaaattcata tgatcgttac
      121 taagactggc tttccgatta cttactcaat cacgaatcca ggtgtccatg acgtgaaagt
      181 gttagagact ttatcggaag aagcgaatct tcccaatatc ctaggggaca aaggatatat
      241 tagccataaa atccatgaaa agcttgccct taagggcatt actat
//
LOCUS       DADXTH010000216          279 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.227, whole genome shotgun sequence.
ACCESSION   DADXTH010000216 DADXTH010000000
VERSION     DADXTH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.227"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          104 a           38 c           48 g           89 t
ORIGIN      
        1 aaaaatatgg tatgcttctt agtaaattca aatatatggt atgcttctta gtgaattcaa
       61 atataaaaga gtttaaagcg taatacactt atggggaagt ggtttggggt gcgctttaaa
      121 ctcttcttta ttattatctc acagcatgct taaaaggtct atggattaag ttatgtaaaa
      181 aagtgcctct ttaaacgtcc taaaatccaa acaaggatag cataatgtac actatcgttg
      241 atccagaaaa caggtaaata aattttgcaa aagcaataa
//
LOCUS       DADXTH010000217          276 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.228, whole genome shotgun sequence.
ACCESSION   DADXTH010000217 DADXTH010000000
VERSION     DADXTH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.228"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(22..267)
                     /locus_tag="IWI97_002827"
     CDS             complement(22..267)
                     /locus_tag="IWI97_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBC2464248.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT           78 a           59 c           51 g           88 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaaca ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcac
//
LOCUS       DADXTH010000218          275 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.229, whole genome shotgun sequence.
ACCESSION   DADXTH010000218 DADXTH010000000
VERSION     DADXTH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.229"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          113 a           30 c           51 g           81 t
ORIGIN      
        1 attcctttaa ggtgtcacta aaaattataa aaaatagggt tgacgcactt ttgatgtcat
       61 gttataataa cgaagtcgtt tggatgaggt agctaataaa gcttgatttt atcgaaaaaa
      121 acttcgaaaa agttgttgac aaataacaat aaagatgata tgataatcaa gtcgctgcta
      181 atgagtgaca aagacgttta gaaaaaataa acaaagaagt tgttgacatt ctcttctgaa
      241 tctgataaga ttaaagagtt gttgaaacaa aaaga
//
LOCUS       DADXTH010000219          270 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.230, whole genome shotgun sequence.
ACCESSION   DADXTH010000219 DADXTH010000000
VERSION     DADXTH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.230"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          107 a           41 c           28 g           94 t
ORIGIN      
        1 caacagtgat gattcctaat aaagaaaaga ctacaataat atttttctaa cttatcgcct
       61 tcaaagaaat agtttcttat ttttcactaa aataaaaaat ttattgcaaa aaaaatagaa
      121 gtgacaaaat tatcaatcat ttcaaggtaa tataactacc tgagtttata gtcaagtcca
      181 gactcctgtg taaaatgcta tacaatgttt ttaccatttc tacttatcaa aattgatgta
      241 ttttcttgaa gaataaatcc attcatcatg
//
LOCUS       DADXTH010000220          266 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.231, whole genome shotgun sequence.
ACCESSION   DADXTH010000220 DADXTH010000000
VERSION     DADXTH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.231"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(36..>266)
                     /locus_tag="IWI97_002828"
     CDS             complement(36..>266)
                     /locus_tag="IWI97_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2464249.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           79 a           38 c           47 g          102 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaataagg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaa
//
LOCUS       DADXTH010000221          262 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.232, whole genome shotgun sequence.
ACCESSION   DADXTH010000221 DADXTH010000000
VERSION     DADXTH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.232"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            81..>262
                     /locus_tag="IWI97_002829"
     CDS             81..>262
                     /locus_tag="IWI97_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2464250.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           83 a           53 c           43 g           83 t
ORIGIN      
        1 tatgaaatat tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta
       61 acgaataaag gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg
      121 ctgccggcgc tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc
      181 aatcaggtga tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca
      241 atgccattgc agaatcaaat tc
//
LOCUS       DADXTH010000222          260 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.denovo.233, whole genome shotgun sequence.
ACCESSION   DADXTH010000222 DADXTH010000000
VERSION     DADXTH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.denovo.233"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            50..>260
                     /locus_tag="IWI97_002830"
     CDS             50..>260
                     /locus_tag="IWI97_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBC2464251.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFT"
BASE COUNT          110 a           43 c           43 g           64 t
ORIGIN      
        1 tacggtacaa tgtttttaac gacaaaaaca tacccaggag gacttttaca tgacccaagt
       61 acattttaca ctgaaaagcg aagagattca aagcattatt gaatattctg taaaggatga
      121 cgtttctaaa aatattttaa caacggtatt taatcaacta atggaaaatc aacgaacaga
      181 atatattcaa gcaaaagaat atgaacgaac agaaaaccga caaagtcaac gaaatggcta
      241 ttatgagcgc agctttacga
//
LOCUS       DADXTH010000223         8504 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.097, whole genome shotgun sequence.
ACCESSION   DADXTH010000223 DADXTH010000000
VERSION     DADXTH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.097"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            167..418
                     /locus_tag="IWI97_002831"
     CDS             167..418
                     /locus_tag="IWI97_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464252.1"
                     /translation="MEYADDRKIQPDRITYRLIQEYIEEKYGFKVHTANIAEVKRNLG
                     LPMYDAPNAVEELKRPYKPAPEYKVEAIKDALRHFKVID"
     gene            complement(562..2109)
                     /locus_tag="IWI97_002832"
     CDS             complement(562..2109)
                     /locus_tag="IWI97_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBC2464253.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(2211..2564)
                     /gene="tnpB"
                     /locus_tag="IWI97_002833"
     CDS             complement(2211..2564)
                     /gene="tnpB"
                     /locus_tag="IWI97_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBC2464254.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2554..2748)
                     /locus_tag="IWI97_002834"
     CDS             complement(2554..2748)
                     /locus_tag="IWI97_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464255.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2816..3280)
                     /locus_tag="IWI97_002835"
     CDS             complement(2816..3280)
                     /locus_tag="IWI97_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464256.1"
                     /translation="MMTMCPRCLELYSEIWSKPCCKCADKTIPVDIELINVVQMLLTR
                     GFDVSYATCYPDKEQGEIEAMEIEIHFRELYPQALFDGLPPDWIVIDEYPVLGGKVLD
                     EPVDILTCAIEYRFEESIHIQKDIAISNLETWLEEKDPQSCRAILTLAVSAP"
     gene            3354..3914
                     /locus_tag="IWI97_002836"
                     /pseudo
     CDS             3354..3914
                     /locus_tag="IWI97_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            4357..5841
                     /gene="lsa(E)"
                     /locus_tag="IWI97_002837"
                     /gene_synonym="lsaE"
     CDS             4357..5841
                     /gene="lsa(E)"
                     /locus_tag="IWI97_002837"
                     /gene_synonym="lsaE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Lsa(E)"
                     /protein_id="HBC2464257.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTECMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            5895..6698
                     /gene="lnu(B)"
                     /locus_tag="IWI97_002838"
     CDS             5895..6698
                     /gene="lnu(B)"
                     /locus_tag="IWI97_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HBC2464258.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            complement(7007..8289)
                     /locus_tag="IWI97_002839"
                     /pseudo
     CDS             complement(7007..8289)
                     /locus_tag="IWI97_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         2781 a         1448 c         1552 g         2723 t
ORIGIN      
        1 cccccatata tcagtttctt aaaaaataat caaaataccg ttgatacgtt cccgcgaacg
       61 aaagggttaa aaatcagctt aagacatcaa tgccatcaac tcgagccacg ttgagacgtg
      121 tgttttattg tcccacaaaa gtgcagacga ttttatcagt gtaaaaatgg agtacgcaga
      181 cgaccgaaaa atccagccag atcgtatcac ctacaggctt attcaggagt atatcgaaga
      241 aaagtacggc ttcaaagttc acactgcaaa cattgcagag gtcaaaagaa acttaggcct
      301 gccgatgtac gacgcaccaa acgcagttga agaactaaaa cgcccatata aaccagcacc
      361 agaatataaa gtagaagcta ttaaagacgc attaaggcac ttcaaggtca tagattaaag
      421 taaagtaccc tttcagcaga tcattactgt tgagagggtc ttactttttc agaagccggc
      481 caatggtaag cgccccatag aacagtacct gtacgtcaaa taacccgacc tatagaatag
      541 aatcgggaat gtttggcgtt tttagttacg agaaaaatgt tgttgtacat ccttgttcca
      601 aggcaacagt tgatctaaag tttctgctga aagtacggga agatttggta tcttatcaaa
      661 tagaaactct agatacttta caggattcaa tccgttaagt tctgctgttt tatatacact
      721 cagaatgatt cctgaagttc gtgctccttc aagacttgtg gaatgcaacc aatttttccg
      781 tcctatcacc agctctttga tatttcgctc cgcacgatta tttgtgagtt ctaaacgacc
      841 atcttcaaaa ataggttcaa aaccacttcg tagtttacac gcatattgaa cggctgcatc
      901 caacttggat tttgcaacag gatctatcgt tgccatccac tcataaaaag caataaataa
      961 tggacgcagt tcttgttgcc gacagcttaa tcgcttctca ggagaaaagt ctttccattt
     1021 cttttctagg acaaaccatt tatccaattg tttgactgct tgagctgcgg gaatatccgt
     1081 gtttttcttt ccttttggaa ttgcttcgaa aaatttccgc cgaacatgcg cgaaacaata
     1141 gacaagtcgt acactttcca cggcattata cccgctatag ccgtcacagt gtaaataccc
     1201 tgtaaactcc ttgagaaaag ttttcggaac atctccagca cgactttcgt cgtggtgata
     1261 ataaacgatg ggatgctgac tgtcttttcc tgtacaaaac tgccagtaat aggtatctgt
     1321 tttggcactt tcaatcacac gataacttgt ttcatccccg tgtagaatct cttcttgatt
     1381 aagatagact cttaatcgtt ctcctaaagg gtctaacgcg ttttgcactg ctttaatatg
     1441 ccaattggtg atattatctc tcgaaatatc caacccatgt gtttgtttcc aatagttctc
     1501 ttgccgatag gcaggaactt tctgaccaaa tttcatccgg atcgtttccg ccactaaact
     1561 attggaacct aagctattag aaatcaaagg tttaggaacg atcgcttttt taaaagaaga
     1621 aagtccttct ttttcgcagc ttttacaaca ataggtatgt tgataatggg tgagacaatc
     1681 caacatagcc tgatggaaac gaatttcttc tcgtactttt gtccgtccga tatctttcat
     1741 ctgtgaaccg caatgttcac aaaattgttc ttccaaagga aggagatgtt ctttctctgt
     1801 tataggtaag tgtttgattt tgtctgcttt cgttcctaca actcgttgat gtccttttac
     1861 tggaataatg gtggcagctg cttctggaac acttacagaa atttcttcat cactaaaaag
     1921 attcaattgc ccattcgttt cctcaaagag agtctcttta gagcgtccaa ataatttgtt
     1981 ggttaaataa ttgacctttt cattcaatag ttggatttca gttgtaagat tctgaatggt
     2041 ttgttgttgt gcttgattgg ttttatgggc ttcttgaagc aactgaagca acgtatctgt
     2101 agtttccaaa atttttcacc accttgaatc gaactgattt tgttacagaa taccacaaga
     2161 aacgcacaag aaataggggt tcaacacatt ttttgtgtta accccccagc ttaaaaaact
     2221 cctttttttc ctggcaaaat agctcgaggt tgatcgatac ttaatccttc taacaaccat
     2281 cgaacctgtt ggttcgttaa ggttcgaact tcgtctttcg tccgaggcca ttttaacttg
     2341 ccgttttcta tccgcttata acagagaaga aaaccatcac cgtcccaata gagaagtttg
     2401 aaacggtctt gacggttccc gcaaaataaa aaaacggcat cttcgtaaag ctcgagttga
     2461 tactcttgtt gaataagact ggcaagacca tcaattcctt ttcgcaaatc tgtttttccg
     2521 cacacaatat aaatatgttt ggtttgaagt aaatcataaa gcattggaca acagttcttt
     2581 caaaatagct tgaagaatat aattgttaat accgttatag atcaaaactt cagaaccatt
     2641 ctctcttttg atgcgcatcg ctaacttggc agccgcgggt ttaccttttt ctggtgcact
     2701 cttttcgttt cctaattgga caggaacaat ctcatttaat ttatacatag aaaacctcca
     2761 gacttcttga tagctcaagt atatcttgag gtgcatttgt tagtaaggta ctgttctatg
     2821 gggcgcttac ggccaatgta aggatagccc tacaagattg cgggtctttc tcttcgagcc
     2881 atgtttccag attgcttata gcgatatcct tttgaatatg gatgctctct tcaaatcgat
     2941 attcgattgc acatgtaagg atatcaacag gttcatcaag aaccttaccg ccaagaactg
     3001 ggtattcgtc aattacaatc cagtcaggtg ggagaccatc aaagagagcc tgcggataca
     3061 attccctaaa gtgaatctca atttccatag cttcgatctc accttgttct ttatctgggt
     3121 aacaagtggc ataagataca tcgaagcctc tagtgagcag catctgaacg acattgatga
     3181 gttcaatatc aacaggaatg gtcttatcgg cgcatttaca acaaggcttc gaccaaattt
     3241 cactgtatag ctctaaacat cgtggacaca tcgtcatcat acaatcactt ccttcaccat
     3301 agttctttta agattaagtg cctgcatagt tgcagttgca gtttgctcgc cagtgcggtg
     3361 atatttaccg ccgggtacac tggaataacc gaggttacaa gtctattgtt tggagatgcg
     3421 tcagccgttt ggaggaaaaa ggatctgaat gtactgcccc taccataaac gaggaaacat
     3481 tgcagacagc agtggttaag gctattaacg aacttttggc taacaaagaa cccttcctcc
     3541 aggcgttgca gaaaaacata gctactatac ttaatgaaga aaatgataat gccaccaatg
     3601 atattgatag caaattggaa gaattacaac aacagcttct tatccaagca aaatccaaga
     3661 atgactatga agatgtggct gatgaaatct accaccttcg agaattgaag caaaatgcac
     3721 ttgttgaaaa tgcagagcgt gaaggaaaaa ggcaacgaat cgctgaaatg actgatttct
     3781 ttaatgaaca atcctgtgag ttagaggaat atgatgagca gttagtaagg aggcttatag
     3841 aaaaagttac ggtatttcat gataagttcg ccgttgaatt caaatcagga gtcgagattg
     3901 atgtagaagg gtaatcttta gaaagagaac agaagaatta tattggattt ttaaagataa
     3961 tatgatataa taaaacgact tggaggtgga agaatgtata acttttcttg ctaattatat
     4021 gatgcataat aagatattta aagaaacggc tatagaaccg tttgttttgt tatgcaaatt
     4081 gcaagaaagt taagtattct acactcaata atacattaca gtaaccttat gttatgtata
     4141 acaaaaggta ataatagtat ttgtttttga gccgtaagaa tttactttct tgcggctttt
     4201 aatatttgta aaagaaagga agataaacgt gatagacaat gatactatgt cattcatgac
     4261 aaaatccttg cagagttgga taacattata gagtaattaa tgccgaaaac cgactttgct
     4321 tataaaattt ttataatgta aaggaggaag aaatatatgt ccttaataaa tgtttcaaat
     4381 ctaacttttt catatgaagg aagttatgac aatatttttg aaaatgtaag ttttcagata
     4441 gatacagatt ggaaactcgg ttttattgga agaaacggac gcggtaaaac tactttctta
     4501 aatttactgc ttggcaaata tgcgtattcc ggcaatataa gttctacagt taagtttgag
     4561 tattttcctt atgatgtgga agataagagt ctatatacaa ttgaagtaat gaagagtatt
     4621 tgtacggaat gtatggattg ggagattttt cgtgaaatat cattgcttga tgttcaagaa
     4681 gatgctttat atcgtccgtt taatacattg tcaaatggtg agcaaacaaa ggtccttctt
     4741 gcagctttat tccttacagc gagttgtttc ctgcttattg atgaacctac aaaccatctt
     4801 gacatcgatg cacgtaatgt agtgcaaaac tatttgaaac gcaagaaggg gtttattttg
     4861 gtatctcatg atagaagctt acttgatcaa tgtgttgacc atatactatc tatcaataaa
     4921 acgaatatcg aaatccaaaa gggaaatttt acttcttggt gggagaacaa aacgttacaa
     4981 gataattttg aactggcaga aaacaagaaa ctccttaaag aaataggaag gttgtcttat
     5041 gcagcaaaac gtagttcaaa ctggtcaaat aaagtagaaa aaagtaaata tggaacaaca
     5101 aattctggtt caaaactgga taagggttat gttggacata aggctgcaaa agcgatgaaa
     5161 cgtgccaaaa atattgagtc aagacatcag gaagccgttt tacaaaaatc agaactgctc
     5221 cacaacattg aacaatatga tgacttaaaa atttcaccac ttgaatttca caaagagtgc
     5281 ttaatagaag cgaatgattt atcattgtct tatggagata aagaagtatg cagtaatctt
     5341 aatttcagag tcaatattgg tgatagagtt gccattatcg gaaaaaatgg gagtggtaag
     5401 tctagtatcc taaaattgat taatggagat gatattaaat ttaccggaaa ttttatgcta
     5461 gcaagtggac taaaaatttc ttatatttcg caagatactt catatttaaa aggtaatcta
     5521 tctgaatttg cctataataa taagatcgat gaaactctat ttaaaacgat tcttcgtaaa
     5581 ctggatttta atagagagca gtttgataag aacatggtgg attttagtgc tggtcagaaa
     5641 aagaaagtac taattgctaa aagcctttgt gaaagtgcac atttgtatat atgggatgag
     5701 ccattgaact atattgatat tttttcacgt atccaaattg aaaaaatgat tttggaatat
     5761 tgtcctacac tattgtttgt ggagcatgat gatgcttttt gcaataacat ttgtacgaaa
     5821 aatattaatt taggtttgta gagattttga gttgccacaa taactaaaag atatttacat
     5881 gaaagggtga agaaatgtta aaacaaaaag aattaattgc aaacgttaag aatcttactg
     5941 agtcagatga acgaattaca gcttgtatga tgtatggatc gtttaccaaa ggagaaggtg
     6001 accaatactc tgatatagag ttctatatat ttttgaaaga tagtataacc tcgaactttg
     6061 attcatccaa ctggttgttt gacgtagctc cgtacttgat gctttataaa aatgagtacg
     6121 gaacagaggt agttattttt gataatctta tacgtgggga atttcatttc ctttctgaaa
     6181 aagatatgaa cataatcccc tcgtttaaag attcaggtta tattcctgat acgaaggcta
     6241 tgcttattta cgatgaaaca gggcaattag aaaattattt atcagagata agtggtgcaa
     6301 gaccaaatag acttactgaa gaaaatgcta attttttgtt gtgtaatttc tctaatctat
     6361 ggttgatggg aatcaacgtt ctaaaaagag gagaatatgc tcgttcatta gaactcttat
     6421 cacaacttca aaaaaataca ctacaactta tacgtatggc agaaaaaaat gctgataatt
     6481 ggctaaacat gagtaaaaac cttgaaaaag aaattagcct tgaaaattat aaaaaatttg
     6541 caaagaccac tgctcgatta gataaggtag aattatttga agcctataaa aattctttgc
     6601 tattagttat ggatttgcaa agtcacctta ttgaacaata caacttaaaa gttacacatg
     6661 acattttaga aagattgttg aattacatta gtgaatagga gagtaacgtt attcacagta
     6721 ttgggtactg aaaattaaca gcatacctca gtttttcaaa aaacagcgaa gcctatggat
     6781 atgttcccgt ctaccgatag tgcaaaaagt gtcgtggata tgtttccgag aacggacata
     6841 cacaaatgac attcattctg tcctctcaat ccatattgaa tgtgtgactg ttttaagtaa
     6901 gtaaggaaaa cataaaaaaa cgggctcttt gtcaaatcgg actgatgaga caaatattga
     6961 agatgatgaa tttgagaatt ttctcaaatt catcatcttt ttttagttat tcaattgtga
     7021 caaggtcgtt tatcataaaa attcttatct tcatattact aaaagatttg aagccataag
     7081 atactctttt taatatctta atatgagtat ttttagcttc tatttttccg ttagaatact
     7141 tatacgttaa gcattacgaa tcccttcttc ataattaatc agattttcaa tcttctgttt
     7201 aaatagtgga tctaaggtag tcggtaagtt ctttaataaa tcaaaaaatg agtctggatc
     7261 tttatttcta aagtgatagg ttaataattg aaacacgcca taggcttctt ttaagtctgt
     7321 agaaaatgat aaaagacgat caatcatcat tgattctgtt agagggggaa acttaggtga
     7381 aggaaagctt ctccaatttt tatattctga actagaaaca gtcatttgat tctttaatag
     7441 gtatttccaa tttactttta gcttattggc ttcagaacgc tggtgattct ttattaatgc
     7501 tttcatttcc ctgactctaa agtcattaaa agcttggttt aagtgtttaa cgatatgaaa
     7561 cctatcaatg actaaagctg cacaaggaaa gattttttta gttaactgaa aataagccgc
     7621 attcatatct gtcactagaa attttacctg ttctcgtttc tccttgggag aacgattgaa
     7681 gtaattcatt agtttatcaa gtctacgact aggaagaaca tcaaccagtt caccggtttc
     7741 accattggca caaataaaac tcattttatc ttcataggtg gcatgagatc tgaattcatc
     7801 aaccattaga acttttggaa aagagcgtgg tttaagcgtt tcaggtaaat gtttttcaac
     7861 agatttaagg acccttataa cggttgtgag tgatacatga catatcttag cgataacggt
     7921 cattgatatt ttttgagtta ataattctag tattctaaat gtcatgaatc gactaataaa
     7981 acaattttct tcaacgatag aacattgagt tgtccaatgt tgatggcagt ttttacaaaa
     8041 atatcgttgt ttttttagct ttaaaatagt tggaatattt tgatatgatt ccaaacgaat
     8101 tgtcacttct tttttcccat tttttacaat ggttttcttt tcatctgcat cgttcgcatg
     8161 agactgacaa tacttacagg ccgtaagctt aggtgagtag gtaccagtga taattaatgt
     8221 gttcacacct ttgtattttc cttcttcaac acaagttaaa tttaaatctt catctttaat
     8281 tcgcaacatt ttttgatatg attagtcatg acatgtcgtc ctttctattt aagattttgg
     8341 ttggtacttt aattttacta gatagacgac atgtttttct gcttaaataa actaaaatag
     8401 ggctgatgaa tcaaaaaatg attcatcagt cctataaatt atagagcctg aatttatccg
     8461 caaaaacaaa ttccgcacct tagaatatga tgataagctt gtaa
//
LOCUS       DADXTH010000224         8471 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.098, whole genome shotgun sequence.
ACCESSION   DADXTH010000224 DADXTH010000000
VERSION     DADXTH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.098"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..366)
                     /locus_tag="IWI97_002840"
     CDS             complement(<1..366)
                     /locus_tag="IWI97_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBC2464259.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKN"
     gene            492..1613
                     /locus_tag="IWI97_002841"
     CDS             492..1613
                     /locus_tag="IWI97_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBC2464260.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            1871..3373
                     /gene="eat(A)"
                     /locus_tag="IWI97_002842"
     CDS             1871..3373
                     /gene="eat(A)"
                     /locus_tag="IWI97_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBC2464261.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            3401..3967
                     /locus_tag="IWI97_002843"
     CDS             3401..3967
                     /locus_tag="IWI97_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBC2464262.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
     gene            4179..5240
                     /locus_tag="IWI97_002844"
     CDS             4179..5240
                     /locus_tag="IWI97_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBC2464263.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5243..6139
                     /gene="murQ"
                     /locus_tag="IWI97_002845"
     CDS             5243..6139
                     /gene="murQ"
                     /locus_tag="IWI97_002845"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBC2464264.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            6153..8135
                     /locus_tag="IWI97_002846"
     CDS             6153..8135
                     /locus_tag="IWI97_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2464265.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGARQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(8205..>8471)
                     /locus_tag="IWI97_002847"
     CDS             complement(8205..>8471)
                     /locus_tag="IWI97_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBC2464266.1"
                     /translation="YLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNKNF
                     NNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
BASE COUNT         2781 a         1512 c         1901 g         2277 t
ORIGIN      
        1 atttttcttt ggtacatatt tttccagtac atcttcatga cctaatccat tcagtaaaca
       61 caaaacataa gtattctcag taataatgga ttggatcgaa cgaaacatat catctaattg
      121 attcgctttt gtcaaggcta cgatcaaatc cacgtgtttg ttgctttcga tgatttcttc
      181 tggtgaatag attggcagtt ttgcaactac ttctttgcca ttaaagtcag caatcaatcc
      241 atttgtacga atggcttcaa tgtgagcagg ccagcgatcg atcaagacga cctcatttcc
      301 gctttgatgc aacattaatc cgattcggct gcccattgct ccggctccag ctattgcgat
      361 tttcatgttt tttctctcct ttattcaatg agttcccttt tagtatatag aaaaacagaa
      421 gaaaatagca ttgttttgtt tgaaaaaagt gaaaaaaagt gtcttactta agaagaaagt
      481 gagggatata gatggatatt ttggcggcaa tcgattcatt taaaggaagt gcttcttcat
      541 ttgaattaaa tcaggcagtt ctaaatggtt gggaagaccg taaaggaaga aaaatcaatg
      601 tgccgattgc agatggcgga gaaaatactg cagaagcaat ctattacgcg ctcgggggaa
      661 cctggcgtac gattgatggg atggatctct tatttcgtca gcgaaaaatc agttatttat
      721 tgacgaactg gcaaggaaaa aataccgcag tcattgaatc tgcacaagta ttaggattag
      781 atttacttga taaaccaacg gatcaaacca tccgacttgc ttctagttat gccttgggag
      841 agcttatcaa agatacactg cttcaagaag tggaacaaat cattgtcact ttaggaggaa
      901 gcggatgtag cgatggtggt ctaggattac tccaatcttt gggtgcttct ttagaagggt
      961 acacagaagg aaatcctttg ttatctacta gaaaacttca tttggatcga aggaacgttc
     1021 ttgtttctaa agtaaaagtg atcatcgctg cagacgtgac caacccatat acaggtcaac
     1081 aaggagctgc ttggcaattt ggaagacaaa aaggaggaac ggatacgacg ttagctttct
     1141 tggataaaca agctgaaaag attgctcagc agataaaaga tgtttgggag gtggatttag
     1201 atgacatccc aggaagcgga gcagcaggcg gtattggcgg agcccttttt ttgttaggag
     1261 cgaagatggt ctctggtttt gagttggtca gccaaatcac tggattagaa aaacagatcg
     1321 aacaagcgga tgtcatcatt acaggagaag ggcggattga tcagcaaaca atccacggaa
     1381 aagttccata tggcgtagca gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt
     1441 gcggaagtag agaccgagag ctggaaggac tctttgattt tctccccatt gtcttaagca
     1501 ttcaattagg accaatcgat ttagaggaag caatggaaca caggcaaaca ttagaaaaaa
     1561 cagaaatctt aggacagaca ctcagcagat ttttcaacat tctcccgaag tagttgacaa
     1621 aaacaatttc tttatgctat cgtaatttca aatataggac aaagtgaggg atccgtgcat
     1681 tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg agacaaggga
     1741 gcagtgtgca gattttcggg attggcttgg ttctttcgat gtatgggtct ttttgtgtgc
     1801 cccaaccctt gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg
     1861 tgatggaata atgtctaaaa tcgaaataaa aaatctgaca ttcggctatg acagccaagg
     1921 cacattatta tttgaacaag ccaatctaaa ctttgacaca caatggaaac taggacttat
     1981 cggacgaaac ggtcgaggaa agacaacttt actgaatatc ctgcaaaaca aactccctta
     2041 tcaagggcaa gtgatccatc agcaagcgtt tgtctatttc ccgcagcaaa caaaagataa
     2101 agagcgtttg acctattatg tgttaaatga gattatggat tttgagatat gggaaattga
     2161 aagagagctc caactgatgc aaacagatcc tgaaatctta tggagagaat tcagcacact
     2221 atcaggagga gagaagacaa aagtcctact ggcactctta tttgtggatg acactcattt
     2281 cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggcagaaaac aagtagcggc
     2341 ttacttgaaa aagaaaaaac aaggattcat cgtggtgagt catgatcggg gatttatcga
     2401 tgaagtagtg gaccatgttt tagcaatcga aaagagccaa ctggaacttt atcaagggaa
     2461 tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatag ctaaaaatga
     2521 aaaattgaaa aaagaagtca gcaggctaaa gaaaacagca gctgaaaaag cagaatggtc
     2581 tcgttcccga gaaggggaca aaacaaagaa aaaagtcgga ttcatcgata cggaatctag
     2641 gcgagtgaat aaaggagcag taggcgctga tgctgcacgg acgatgaaac gatctaaagc
     2701 aatcgtgaat cggatggaaa cccagatcag tgagaaagaa aaattattaa aagatatcga
     2761 gtatatcgat gctttgacga tgaatagcca agtgtctcat cataaacggc tgttaagcgt
     2821 agaaaatctt caattagggt atgaaaatct gttattcgag ccagttcatt ttacgatcga
     2881 acctcatcag cggatggcaa tttcaggtcc taacggcgcg ggaaagtcat ccattatcca
     2941 ttatcttctg gggacattca acggcaaggt tgtaggagaa aaatcccagc ccaaacattt
     3001 gaccattagt tacgtacgcc aaaattatga agacaatcga gggacattgg cggaattcgc
     3061 gaagaaaaac cgagtagact atcaagcatt tttgaataat ctccgaaagc ttgggatgga
     3121 aagagatgtt tttcataata aaatcgaaca gatgagtatg ggacaacgga aaaaagtgga
     3181 attggctaaa tctttatccc aaccagctga gctctatata tgggatgaac cattgaatta
     3241 tttggatgtc ttcaatcaag aacaattaga acaactgatt ttgaacgtga aacctgctat
     3301 gttactagtg gaacatgatc aaacctttct agataaagta tctactgaga ttatttctct
     3361 tgagaaaatc taaaccttgt aatctcaaag acaacaaggt atggttaagg agaactttgt
     3421 caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa cttggaacgc
     3481 acaaggatta gtttgcgggc gagcagatgt gccgctgact gaaaaaggac agatgcaggc
     3541 acaggcacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa tcatccattc
     3601 ccctcttcaa cgagcaagag atacagcaca agcagtagtt gatcgtctca atttgccgat
     3661 gaccgctgat gagcgattgg tagaaatgga ttttggggat tatgacggac tgccaagcaa
     3721 agatgaaaac tttcaaaaag cgcgtttagc gtttgctgta cgtttcccaa atggagagtc
     3781 ggtcttagat gtgtacgcac ggatcgttcc tttattgaaa gaatgcatgg aagacgaaga
     3841 aaatgtctac ttgttagtct gtcataatgc cttgataaga gtgatcaatg cttattttca
     3901 tccaatgcct aatgaagggt tctttacatt catggtagac aatacagaat taatttctta
     3961 tgagtaatta gacttttgtg ttatttaaat aaaatgtttc aaggcactgc ttttgcttag
     4021 cagtgccttt tttgtggctg tgtcaattct tttggcatta taaatccaat ttttagaaat
     4081 aaaatttaat tattttaaca aaagtattga aattttgttt ctttagatat atactctagt
     4141 catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt tcaatattta
     4201 tgaatgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca tccaatggct
     4261 ttgtcggtat tttcacttcc atgcatatac cggaagatga tgtttcggct tatcaaaatc
     4321 gattgatcga actaggaagc gaagcgaaaa agaataaact agagttgatg gtcgatattt
     4381 caggagaggc attgaaacga gctggctttt cattagaaga tctaacaccc ttgaaagcaa
     4441 ttggtgtaac agggttaaga atggattatc atatttctaa tcagcagatc gccgactggt
     4501 cacatcaatt gaaaatcagt ttgaatgcca gcacgatcac gcctaatgat atcgatgagt
     4561 tgaaagaagc agcggctgat ttctcacaga ttgaagcttg gcataattat tatccacgtc
     4621 ttgaaacagg gttggacaaa gaatggtatc aggaaaaaaa tcaatggctc aaaactcaag
     4681 gattccttgt acaaggcttt gtaccaggag atacagagtt gagaggtcca ctgtatcaag
     4741 gattaccaac tttggaagaa catcgaggga tccatcctct ggcaggtgct cttgatctat
     4801 tagcaagtaa cacggatatt gtttatatcg gagatgcagg tctgtcagaa cacgttcagg
     4861 agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa cctgtagatg
     4921 aagtttttta cgaatatata ttaggaaaac atacgaatcg gcaagatgat gcaagagatg
     4981 tcatacgaag cgcagatgcg cgttttcgag agattccgcc aatcccagta agaaatacag
     5041 ctaagcgtat gaaaggatct attactttag ataacgaaaa gtatttgcgc tatatgggtg
     5101 agatccaatt aactaaatat gatttgccag cggatgaaaa agtgaatgta gtagccaaag
     5161 tcatcaaaga agaactaccg ctgatcaacc aaatcaaagc tggtatgaac tatcaattta
     5221 taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa caacggaacg
     5281 cagaaaccaa gaaacttttg gattagatga aatgagtatt tctcaagcag tccaactcat
     5341 gaacaaagaa gatcagaaag tggccgctgc tgttgaaaaa gaactgccaa gtatcgagcc
     5401 agtgatcgcc aacacgattg aatcctttaa aaaaggtggt cgcttgattt atatgggagc
     5461 tggaacgagt ggtagacttg gtgtattaga tgctgctgaa tgtgtaccga cattcggtgt
     5521 agaaccggaa atggtcatag gcctaattgc tggtggagaa gaagcaatga ctgtagccgt
     5581 cgaaggagcc gaagattctt atgaattagg gcaagaagat ctaatcaagc tgaatttaac
     5641 aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg tgattggtgg
     5701 attagattac gcaaaatcga ttggtgctgt tacaggaacg atttcttgca ataaacaagc
     5761 agaaatcagc gagcatgcgg atttcccaat cgaagtcgat gcaggaccag aatttttgac
     5821 aggctctact cgattgaagt caggaacagc acaaaaactg attttgaata tgatttctac
     5881 tatcagtatg atcggtatcg gtaaagtatt caataacttg atggtcgacg tcaaaccaac
     5941 aaatgaaaaa ctaattgaac gctcaaaacg aatcatcatg caagcgacag aagctgacta
     6001 cgaaacagct gaaaagtact ttgaggaagc agatcaaaat gtcaaactgg ctatcgtcat
     6061 gattttgaca aatagtgata aagaggcagc tcaagcaaaa ttagctgaag caaacggttt
     6121 tgtaaaagga accttatgag ggggaaaaga ccatggcaaa aaacgaattg actttggaag
     6181 aactggcaaa acaaatttat caaggagcag gcggtatggg caatgttgaa agcattgtcc
     6241 actgcatgac tcgtgtacgt atgaaggtca tcaatgatga tcttgtaaat gtctcggaat
     6301 taaaagcgat tccaggagta cttggcgtag tagaggatga acagctccaa gtcatcatcg
     6361 gacctgggaa agtgaacaaa gtagcgcagg aaatggttga cttagcaggc gtgaaattag
     6421 gagaaaaact tccttctgct agtgcaacta cagcaagttc ttctgcatct ggaaaagacc
     6481 gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa aaaccctcta
     6541 aaatcaaatc ggtattaaaa gacatctcta atatctttgt accaatgatc cctgcatttg
     6601 tcggcactgg gatcgtggca gggattgccg cagttttagc aaacttagtc acagctggaa
     6661 cactagacgc aggaacatgg caacaatata tcgatgtgat gaacatcttg aaaaacggga
     6721 tgtttagcta tctagttatc tatacaggta tcaatgctgc acaagttttt ggtgcaacgc
     6781 caacgcttgg tggagttatc ggggctgttg tgatgctgac agggatgaat ccagaagcac
     6841 cactgaaaaa cttgtttacc ggtgaagctc ttgcaactgg acaaggcggg atcttaggtg
     6901 ttatcttcgc agtttggtta ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg
     6961 atgcggtaga tatcatcgtt acgccaactc tttccctgat tgccatcggt ctgatcgaaa
     7021 ttttcttcat catgccttta gcagggttca tttctgacgg catggtcggt gggatcaact
     7081 gggtacttgg tgttggagga gcatttgccg gcttcgttct aggtacatta ttcttgccaa
     7141 tggtcatgtt cggactacat cagatcttga cgccaatcca tgtacaaatg attgatgaaa
     7201 ctggtcgtac cttattgctt ccaatccttg cgatggccgg agcaagacaa gtgggtgcag
     7261 cattggcatt atggatccgc tgcaaaaaag acaaagaatt aacagaaatg atcaaagggg
     7321 ctttaccagt cggaatctta ggaatcggcg aaccattgat ttacggagta acattacctc
     7381 taggacgtcc atttattact gcttgtatcg gtggaggtat cggtggggca gtgatcggaa
     7441 tgatcggaaa tgtcggagca atcgctattg gaccttcagg agccgctttg attccattga
     7501 tctctgatgg taaatggtta ggctatatcc ttggtttatt ggctgcttac gctggtggct
     7561 tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa gaactagcga
     7621 ccgaaacagc cacagtaaat gaagtacctg aaaaagcagt acctcaagct tcagctgaaa
     7681 gtgtgactat cttatcatca gttgctgacg gaacagttgt ttctttagat aaagcaagtg
     7741 acccagtttt tgcacaaaaa atgatgggtg aaggttactt tgtagaacca gaaaacggac
     7801 atatctattc cccggttact ggagtaataa gttctgtttt cccaacaaaa catgctatcg
     7861 gcattacgac accaaatggc ttagaaatct tgcttcacat gggcatcaac acagttgact
     7921 taggcggtac gccattcgat gtgaaagtaa cagaaggaca gcaagtgaca cctgatacgc
     7981 ttgtagcaga agctgactta gaagcaatca aagcagcaga caaagaaacc tccatgatcg
     8041 tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcga
     8101 aagcaaaagc acctgtgatg gatgtcaaag cttaataaga atagaaacaa tagagaacat
     8161 aaaaagaaga agccgaaatt tatttttcag cttcttcttt tgtgttattt atttttttct
     8221 gccaaaattt gataaacgcg tgtcacatca gatggtgttc cccgtaattc ataatctgcc
     8281 aagaaaggaa agccgaatct ttgtgcgtat tgtttggctg tcagacaata ttgattgttg
     8341 aagtttttat taccgctacc gactacacca aggcaaagtt tatgattgtc ttcgtattcc
     8401 agatattcgc gcatcgtttc ggtcaagatt tctgtgtcac cgttgtctaa cccattcccg
     8461 ccgtctaaat a
//
LOCUS       DADXTH010000225         3720 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.132, whole genome shotgun sequence.
ACCESSION   DADXTH010000225 DADXTH010000000
VERSION     DADXTH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.132"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(531..656)
                     /locus_tag="IWI97_002848"
     CDS             complement(531..656)
                     /locus_tag="IWI97_002848"
                     /inference="COORDINATES: protein motif:HMM:NF019687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2464267.1"
                     /translation="MLFPPFKSSKSRKNPSDKYSYHWDFNTKIAVYYTLIATDAL"
     gene            656..2581
                     /gene="tet(S)"
                     /locus_tag="IWI97_002849"
     CDS             656..2581
                     /gene="tet(S)"
                     /locus_tag="IWI97_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063856411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HBC2464268.1"
                     /translation="MKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSGTTKTD
                     TMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAILLISAK
                     DGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIKQTVNL
                     NLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSLYPVYH
                     GSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLYSGTLH
                     LRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGDKKRLP
                     HREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIVLSFLG
                     EVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASIGLSVT
                     PLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKYCANIL
                     NTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAFQPRRP
                     NNRIDKVRHMFNKINLH"
     gene            2669..2836
                     /locus_tag="IWI97_002850"
     CDS             2669..2836
                     /locus_tag="IWI97_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBC2464269.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(3350..3706)
                     /locus_tag="IWI97_002851"
     CDS             complement(3350..3706)
                     /locus_tag="IWI97_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2464270.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
BASE COUNT         1327 a          577 c          618 g         1198 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaacccttt taatgatatt ttccaagtca
       61 aacttaatat taacaattca aaactattgg aaccactgca aaatattttg ttaagcagta
      121 tatatttcaa aaattatacg taatatattt gtagaaaggt tgtggacagt tgtcctattg
      181 acctgaaaag taatataata gctggatgaa taaagaatcc cttatgaact tgtaaatcgt
      241 tattaaatcg ctgaattttg atagccattc tgaagagtta tcttcaacta ctacactaac
      301 ttccattaat ttctcattaa tttccctttc gttcaaaaaa tatggttggc tttacaagca
      361 ttctatcata tgatagaatt aagataacat tcaaaaggag ataataaaaa tgcggcaagg
      421 tattcttaaa taaactgtca attagatagc gggaataatt aattaagcat ccttatttaa
      481 aataaggact ggttttttgt gcccagttta agaatacttt tatcatttta tcataaagca
      541 tctgtggcta tcagtgtgta gtatacagct atttttgtat taaaatccca gtgataagag
      601 tatttatcac tgggattttt tctgcttttt gagcttttga atggaggaaa taaaattgaa
      661 aattattaat atcggtatct tagcacatgt tgatgcagga aaaactactt tgacagaaag
      721 cttactatac agtagcggag caattaaaga gttaggaagt gtagatagcg gtacaacgaa
      781 aacggatact atgtttttgg aacgccagag aggtattact attcagaccg caataacatc
      841 ttttcaacgg gaaaatgtta aagtaaatat tgtagatact cctggacaca tggatttttt
      901 ggcagatgta taccgttcat tatctgtttt ggatggagct attttgctaa tctctgcaaa
      961 agatggagta cagtcacaaa ctcgtatact attccatgca cttagaaaga tgaacatacc
     1021 tataatattt tttattaaca aaattgatca aaatggaata aatttgccag atgtttatca
     1081 agatattaag gacaaacttt ctgacgacat cataattaag cagactgtga atctaaattt
     1141 gaaaccttat gtaatagatt atactgaacc agaacaatgg gagacagtaa ttgtgggaaa
     1201 tgattattta ttagaaaaat ataccattgg gaaaacattg aatattgcag aacttgaaaa
     1261 ggaggaaaac gaaagaattc aaagttgctc cttatatcct gtttatcacg gaagtgcaaa
     1321 gaataatatt ggaattaaac aacttataga ggtaattact agcaaattat tttcacccac
     1381 acaactcaat tcagataaac tttgtggaaa tgtttttaaa gtagaatatt cagatgatgg
     1441 tcaacggctt gtctatgtac gtctttatag tggaacgcta catttgcgag actcagtcaa
     1501 tatatcagaa aaggaaaaaa taaaagttac agaaatgtat acttcaataa atggagaatt
     1561 acgccagata gataaggcag agcctggtga gattattatt ttaaaaaatg agcttttaaa
     1621 actaaataac gtacttggag ataaaaaaag attaccacat agagaaattc ttgagaatcc
     1681 tcttcctatg ttacaaacaa caattgaacc atgtaaatca gtacaaagag aaaagttact
     1741 agatgcactt tttgaaatat ccgatagtga tccccttcta caatattatg tagatacagt
     1801 aactcacgaa attgtgctat cttttttagg tgaggtccaa atggaggtaa cttgtactct
     1861 gattcaagaa aaatatcata ttgagataga aacaagaaaa ccaactgtca tttatatgga
     1921 aagaccatta aaaaaatctg aatttaccat tgatatcgaa gtacctccaa atcctttctg
     1981 ggcttctatt ggtttatctg taacaccact tcctttgggt agtggcattc agtatgagag
     2041 cctggtttct ctaggttatt taaatcaatc atttcaaaat gcagttatgg aaggtatacg
     2101 ctatgggtgt gaacaaggat tgtacggttg gaaattaaca gactgtaaga tctgttttaa
     2161 gtatggtcta tattacagcc ctgtcagtac gccagcagat ttccgaatgc ttgcgcctat
     2221 tgtactagag caggctttta gaaagagtgg tacagagtta ttagagccat atcttagctt
     2281 cgaaatttat gtaccacaag aatatctttc gagagcatat aatgatgctt ccaaatattg
     2341 tgcaaatatt ttaaatacta agttaaaagg taacgaggtc attctcattg gtgaaattcc
     2401 agcccgttgt attcaagagt atcgaaacag tttaactttc tttacaaatg gacgcagtgt
     2461 ctgtttaaca gagttaaaag gttatcaggt tactaacatt aagtctgctt tccaaccacg
     2521 tcgtccaaat aatagaatag acaaagtaag gcatatgttt aataaaatca acttacattg
     2581 atagtgaact aaagagaaat ttttaaaatg aatattgagt tgttaattag aacaggagga
     2641 tatagatatt gtaaatacta ggtggatact gtgccctata tgtggcaata agacccgttt
     2701 aaaaatgagg gaagatactg aattaaaaaa tttccctcta ttttgtccta aatgcagaca
     2761 agaaatttta attgaaataa cgaagtttag aataactgta attacagagc cagacgcaaa
     2821 gacgcagagc cgataattcg agatatcaaa atctcatttt atcggctttt tcttttctat
     2881 gcataccatt gagcttagtt cttgatatcc tttataagaa aataagtgta ctgatttttt
     2941 ttgcagtagc tgtattacag tagacatccc tcaagcataa aaagtataca attaaaattc
     3001 aatacttgat tattgatgaa gtacagagtt actcgtcatt tcaaaactag tatatcagtc
     3061 aagtttttcc taaggaacaa aaacgaactt cgctatggga atcaaaaatt tttagtcgga
     3121 tgaagtgata tgcaataaat ccagaaaatt ttcccaacaa caaaaaatga actgtacttt
     3181 gcagcgataa gagtaaccaa agaactattt atcattcgct ctagttccta agtgtaacaa
     3241 agataaattc attttctatt tatgattaac aaaaatcaga aaaaattcat tacatctttg
     3301 taatcaatct ttcctgatag tattaattaa tccattgtgt attattgaac tagttaaatt
     3361 tacatttttc tccgtaaatt gcttgaatca ctttcccaat cgtcactaat tcatcttcat
     3421 ccagacaatc caactgtgct tctacttgaa gacgtttgga gcttttatga ctgttttctt
     3481 cccctaaaat aaatccatcc aaagaaatat ttagcaaagt cactaaatca tatacaacat
     3541 gaaaacttgg ctgttgtcct ttattttcaa tgttcgtcaa atagcgtggc gcaatctgga
     3601 tcattgatcc tacctgctca cgagtcaatc ctttactaag acgggcttct ttaatcgcaa
     3661 gacccaatgg tttgaaatca aaatttgata gagtagtttg ccatattctt gcacccaacg
//
LOCUS       DADXTH010000226         3030 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.139, whole genome shotgun sequence.
ACCESSION   DADXTH010000226 DADXTH010000000
VERSION     DADXTH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.139"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            81..545
                     /locus_tag="IWI97_002852"
     CDS             81..545
                     /locus_tag="IWI97_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2464271.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            529..1158
                     /locus_tag="IWI97_002853"
     CDS             529..1158
                     /locus_tag="IWI97_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBC2464272.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1512..2990
                     /gene="msr(C)"
                     /locus_tag="IWI97_002854"
     CDS             1512..2990
                     /gene="msr(C)"
                     /locus_tag="IWI97_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBC2464273.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT         1065 a          499 c          635 g          831 t
ORIGIN      
        1 attctttgac ttttttcttt ttttattcta ttattgacaa gtcaatcgtt gacgagtcaa
       61 ttattagaaa ggaggaaatt atgtctttag aagagattag tcacctttta tatcaaatca
      121 aattagccga tcaaaaaatc gtccagatct ttgaagaaaa tatcggtata agtcttacta
      181 gatacgagat catgatgaga ctgaaagaag aaaatccatt gctacaaagc agtttgcaag
      241 aaaagttgca gattgatcaa ggagcagtta ctcgtcatct gaaggtattg gaagcaaatg
      301 gctatgtttc gcgtcagaga aatcctcaga acaaccggga agtgtatgta gcgctaacag
      361 aaaaagccca gaagaaaatc gagcattgtg aaatggacca taaagataca ggttccgtgc
      421 tatccccggc tttcagcgat gtagaaatga aacaatttct aacactttta gataaattga
      481 atacccaact ttgcaatata acaacagaaa cgaaagaggt taataataat ggaacaaaaa
      541 aatgattttt caacaattat gactggtaga agatccgttc gcgtatatga tgaaacatac
      601 aaaatcccac acgaagaaat gctggaaatg atcaaagaag cgacgactgc accttcttct
      661 gtcaatatgc aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg
      721 cgtcctttga tccgtttcaa tacgagacaa aatgatactt cttctgcaat ggtcatgatt
      781 tttggcgata tgcaatgtta cgaatatggc gaagaaatct ataaccaagc ttacgaatca
      841 gggaagatgc caaaagaagt cagagatcaa caattagctg cgatcattcc ttattacaag
      901 agtttttcaa gagaagaaat gaacgatgta gtcaaagtcg attctagctt agcggctatg
      961 caattcatgt tagttgcgcg tgatcatggc tatgaaacaa atccaattgg tggattcgaa
     1021 gcagatcagt tggcagaggc atttggtttg gataaagacc gttatgttcc agttattatt
     1081 ttatcaatag gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc accagaaaag
     1141 atcactacgt tcgaataaaa ggttttcagt tactattcag tagctattgc tttttttgaa
     1201 tcgtgtgtgg taacctaata aaaaaggagg tcggaaaaat gactgcatcg atgaaattac
     1261 gtttcgaact tttgaataac aactaaataa attcaaaaga catgtataaa caacaatttc
     1321 atggatggag tatgtccatt ttttcgaacg acagtagaat aggtgaaagc tatgacagat
     1381 ttggtgattt agtcatagtt atttcgttca gagtaggaaa agatgtgatg cagttttttt
     1441 gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca tgaaatagga
     1501 gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc agctttggca
     1561 accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt atagggatca
     1621 taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt gaactttttc
     1681 ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa ttaacctatc
     1741 tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta agactggaag
     1801 aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg atacgtcttc
     1861 tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta gatagaaaaa
     1921 gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt tttgttagtc
     1981 atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa
     2041 tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg gagaaaaaga
     2101 ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa aaatcgatcc
     2161 aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac
     2221 aaatcagacc ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc
     2281 aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca accaagccgc
     2341 agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac agccgctatc
     2401 ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg gtaaatggtg
     2461 attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga
     2521 caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa
     2581 gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc atcattcgtt
     2641 ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg aatcacttag
     2701 ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga gaagcgaccc
     2761 gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta gatgaaccga
     2821 ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa atatatccgg
     2881 gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct gatgaagttt
     2941 atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa atgtgctacg
     3001 atgattttcg tgacaaattt aaaggagtgg
//
LOCUS       DADXTH010000227         2624 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.148, whole genome shotgun sequence.
ACCESSION   DADXTH010000227 DADXTH010000000
VERSION     DADXTH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.148"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..179
                     /locus_tag="IWI97_002855"
                     /pseudo
     CDS             1..179
                     /locus_tag="IWI97_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            210..1085
                     /locus_tag="IWI97_002856"
                     /note="aph(2'')-Ia"
     CDS             210..1085
                     /locus_tag="IWI97_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBC2464274.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1173..2345)
                     /locus_tag="IWI97_002857"
     CDS             complement(1173..2345)
                     /locus_tag="IWI97_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HBC2464275.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          927 a          367 c          463 g          867 t
ORIGIN      
        1 tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta gaccctcata
       61 aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt attgaagatt
      121 tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg gaatatagat
      181 atgatgataa tgccacaaat gttaaggcaa tgaaatattt aattgagcat tactttgata
      241 atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg gcatatttag
      301 ttaataatga atacattttt aaaacaaaat ttagtactaa taagaaaaaa ggttatgcaa
      361 aagaaaaagc aatatataat tttttaaata caaatttaga aactaatgta aaaattccta
      421 atattgaata ttcgtatatt agtgatgaat tatctatact aggttataaa gaaattaaag
      481 gaactttttt aacaccagaa atttattcta ctatgtcaga agaagaacaa aatttgttaa
      541 aacgagatat tgccagtttt ttaagacaaa tgcacggttt agattataca gatattagtg
      601 aatgtactat tgataataaa caaaatgtat tagaagagta tatattgttg cgtgaaacta
      661 tttataatga tttaactgat atagaaaaag attatataga aagttttatg gaaagactaa
      721 atgcaacaac agtttttgag ggtaaaaagt gtttatgcca taatgatttt agttgtaatc
      781 atctattgtt agatggcaat aatagattaa ctggaataat tgattttgga gattctggaa
      841 ttatagatga atattgtgat tttatatact tacttgaaga tagtgaagaa gaaataggaa
      901 caaattttgg agaagatata ttaagaatgt atggaaatat agatattgag aaagcaaaag
      961 aatatcaaga tatagttgaa gaatattatc ctattgaaac tattgtttat ggaattaaaa
     1021 atattaaaca ggaatttatc gaaaatggta gaaaagaaat ttataaaagg acttataaag
     1081 attgattata taatatatga aaagctatta taaaagacat tagtcaagtc cataatcctg
     1141 tgtaaaatac tatacaatgt tttaccggtc tctcattgat caaacttaat atattttctt
     1201 gtagaactga gccattcgtc atgaaggtcc ataaggacag ctccaattaa tcgattggca
     1261 gacgttcggt tgggaaaaat ccgaataatc ttttctcttc tgcggacttc ctggttcagt
     1321 cgttcaagaa ggttggtgct ttttagccga ttatgactat ttccgataac cgtgtattga
     1381 aaggcatctt cgaagccatt atccaatatt tcgcaggctt ttgtatattt aggctggtcg
     1441 atatattcac cgactaaggc attcttagct gttcgagcga gttcaatatc cgtaaactta
     1501 aagatcgctt ttactgcttc tctaaacggt tttgaattct tttttggaat ggaactgaag
     1561 atgtttctta aaaaatggac ctggcatctc tgccaacttg cgttggtaaa tgacttacga
     1621 atcgcagaca ctaggccttt atgggcatca gaaatgatga gttctgtccc ctttaggccg
     1681 cgttctttta agtattcaaa gaagatggac catgtgtcat cactttcttc attttgaatc
     1741 atgaagccaa tgatttcacg gtcgccacct tctgttatcc cgatcgcaat atggcagctt
     1801 ttagaaagca ctcgatggtc ctcacggact tttatgtaga gaacatcagt catcagataa
     1861 ggataattcg tacctgagag tgaacggttc tgccattcgt tgaccatcgg gtctaactgc
     1921 tcagtcaggc tagaaacaaa agatttcgat acagattttc cacatagctc ttcaacaatc
     1981 tttgaaactt tacgtgtcga aacgcccgaa acatacatct caagcattga agcgagcagt
     2041 gccttttcat ttcgctgata acgctcaaac accgtcggtg aaaattcacc atcacgtgtt
     2101 ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa agtctcgctc ataatagcca
     2161 tttctttgac tctgacgact ttctgaacgt tcatagtcat cggcttgaat atattctgtt
     2221 cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa tatttttaga aacatcatct
     2281 tttaccgaat gttcaataat actttgaacc tcttcgttgt tcagtgtaaa atgtacttgg
     2341 gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa acattgtacc gtaaaagggc
     2401 tgttacatgg ccttttatct tttacacaat tatatggact ttatcaagac attagtatta
     2461 aatagtttaa aaaaatgaaa aataataaag gaagtggaaa gatggattta gaaaaaacaa
     2521 ataataattc aatgattatt aaatatcaaa atacagataa tgtattagaa gatatatgtt
     2581 ctataagatg aatttcagga ttatgatatt acatattttg taag
//
LOCUS       DADXTH010000228         2452 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.150, whole genome shotgun sequence.
ACCESSION   DADXTH010000228 DADXTH010000000
VERSION     DADXTH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.150"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..890
                     /locus_tag="IWI97_002858"
     CDS             27..890
                     /locus_tag="IWI97_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBC2464276.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYINKYID
                     EDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITRYTED
                     MYKKYVENDYF"
     gene            934..1461
                     /locus_tag="IWI97_002859"
     CDS             934..1461
                     /locus_tag="IWI97_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBC2464277.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            1593..2402
                     /locus_tag="IWI97_002860"
     CDS             1593..2402
                     /locus_tag="IWI97_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(9) family aminoglycoside
                     nucleotidyltransferase Spw"
                     /protein_id="HBC2464278.1"
                     /translation="MSIDLSNKKIPKEAIQALKTIAELLDNMLIGVYLYGSAVMGGLR
                     MNSDVDILVITNQSLSEKTRRNLTNRLMLISGKIGNIKDMRPLEVTVINQKDIVPWHF
                     PPKYEFMYGEWLREQFEKGEIPESTYDPDLAILLAQLRKNSINLLGPKATEVIEPVPM
                     TDIRKAIKESLPGLIASINGDERNVILTLARMWLTASTGEIRSKDLAAEWAIPQLPDE
                     HATLLNKAREAYLGECVDKWEGMESEVAELVNHMKKSIESSLNIQLPFRIV"
BASE COUNT          883 a          317 c          518 g          734 t
ORIGIN      
        1 agaaaaaata aacgaggagt atgtaaatga gatcagaaaa agaaatgatg gatttagtac
       61 tttctttagc agaacaggat gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa
      121 atattaatat acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata
      181 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
      241 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
      301 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
      361 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
      421 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
      481 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgcaaag
      541 agatactgtt tgcaatcgat catctgaacc agattctacg gtttgaacta cttaggatga
      601 tgtcgtggaa ggttgggata aagacagaat tttcattaag tgttgggaaa aattataagt
      661 atattaacaa atacattgat gaagatctat ggaatagatt attatctaca tatcgcatgg
      721 attcctatga aaatatttgg aagtcattat ttatatgcca ccaattgttc agggaagtgt
      781 ccaaagaggt agcagaacta ctggggtttg attatccaga gtatggtaag aacataacaa
      841 gatataccga ggacatgtat aaaaaatatg ttgaaaatga ctatttttaa agattagcaa
      901 agattattaa ccataagcaa ggggtgtaaa tgtatggaaa gtactgatgt tataaatgaa
      961 attagaggta tattacctcg taactcaatt ggaaaatatg atttactaac tatttttaca
     1021 cataaaaccg tttttgataa aattgtaaaa gtactatcat tggattttca aaataaagta
     1081 gattatgtgg ctgccccaga agcaattgga tggatactag gcactgccat agccaaagaa
     1141 cttggagtgg ggtttattgg agtaagaaaa ggtgataagc tgccatacgc aaaagaagag
     1201 attatttcga cacattttac cgactattca ggtcaagata aaagctttga aatatctaag
     1261 agttctattg tgaggaacaa aagagttttg attgtagatg attggataga gacaggttct
     1321 caaatgaagg ctttaatagc tttgttagag aaattggatt gcagcattat tgggttggct
     1381 actattggga ttgatataaa tgaggttact caaaaatgga tagacagcag ttttgtagct
     1441 tatattggct caaacatata ataaaccgaa agaaaatctt gaatcaaaat tttggatggc
     1501 gttaatttac aatacaacga aagatgttta ctcttttttt ataaatttta agaggcgtta
     1561 aaaaacagtt ttcagacgaa aggagatcga agttgagtat agatttaagt aacaaaaaaa
     1621 ttccgaaaga agcaattcaa gcattaaaaa ctatagcgga actacttgat aatatgttaa
     1681 ttggggtgta tttgtatggt tcggcagtaa tgggtggttt acgtatgaat agcgatgtag
     1741 atattttggt aataacaaat caaagtttat ctgaaaaaac tcgaaggaat cttacaaata
     1801 ggttaatgct tatatctggg aaaataggaa acataaaaga tatgaggcct cttgaagtta
     1861 cggtcataaa tcaaaaggat attgtccctt ggcatttccc ccccaaatat gaatttatgt
     1921 atggcgagtg gctaagagag cagtttgaaa agggagaaat tcctgagtcg acttatgatc
     1981 cggatttagc aatactttta gcacaactaa gaaaaaatag tattaacctt ttgggaccaa
     2041 aggcaacaga agtaattgag cctgtgccaa tgacagatat tcgaaaagca attaaagaat
     2101 cgttgcccgg gttgatagct agcattaacg gtgacgaacg caatgtgatt ttaactttag
     2161 ccagaatgtg gctgacagca tctactggtg aaattcgctc aaaagatctg gcagctgaat
     2221 gggcgatacc tcaattaccc gatgagcatg ctactttact caacaaagcg agagaggctt
     2281 atttaggaga gtgtgttgac aagtgggaag gaatggaatc tgaggtggct gaactcgtta
     2341 atcatatgaa aaagtctata gagtcttccc ttaatatcca attacctttt cgaatagttt
     2401 aaatataaca aagactagtt gttaatgtgc attattgatt gcttcgatat gt
//
LOCUS       DADXTH010000229         2379 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.151, whole genome shotgun sequence.
ACCESSION   DADXTH010000229 DADXTH010000000
VERSION     DADXTH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.151"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..935
                     /locus_tag="IWI97_002861"
     CDS             27..935
                     /locus_tag="IWI97_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBC2464279.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            932..1474
                     /gene="sat4"
                     /locus_tag="IWI97_002862"
     CDS             932..1474
                     /gene="sat4"
                     /locus_tag="IWI97_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBC2464280.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1567..2361
                     /locus_tag="IWI97_002863"
                     /note="aph(3')-IIIa"
     CDS             1567..2361
                     /locus_tag="IWI97_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBC2464281.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          805 a          366 c          566 g          642 t
ORIGIN      
        1 agaaaaaata aacgaggagt atgtaaatga gatcagaaaa agaaatgatg gatttagtac
       61 tttctttagc agaacaggat gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa
      121 atattaatat acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata
      181 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
      241 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
      301 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
      361 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
      421 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
      481 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg
      541 aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga
      601 tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt
      661 ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg
      721 attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat
      781 ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa
      841 aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat
      901 atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct
      961 gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
     1021 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
     1081 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
     1141 tgataagata atatatcttt actaccaaga cgataaatgc gtcggaaaag ttaaactgcg
     1201 aaaaaattgg aaccggtacg cttatataga agatatcgcc gtatgtaagg atttcagggg
     1261 gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt
     1321 gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa
     1381 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     1441 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     1501 cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa
     1561 taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc
     1621 tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat
     1681 gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa
     1741 cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac
     1801 tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc
     1861 tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc
     1921 atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc
     1981 cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac
     2041 tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa
     2101 aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg
     2161 aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg
     2221 tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc
     2281 gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt
     2341 ttactggatg aattgtttta gtacctagat ttagatgtc
//
LOCUS       DADXTH010000230         1929 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.158, whole genome shotgun sequence.
ACCESSION   DADXTH010000230 DADXTH010000000
VERSION     DADXTH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.158"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..192
                     /locus_tag="IWI97_002864"
     CDS             <1..192
                     /locus_tag="IWI97_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2464282.1"
                     /translation="EWETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETE
                     AQLEEKMARWEYLSEWVED"
     gene            204..1151
                     /locus_tag="IWI97_002865"
     CDS             204..1151
                     /locus_tag="IWI97_002865"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBC2464283.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1171..1698
                     /locus_tag="IWI97_002866"
     CDS             1171..1698
                     /locus_tag="IWI97_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBC2464284.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1761..>1929)
                     /locus_tag="IWI97_002867"
     CDS             complement(1761..>1929)
                     /locus_tag="IWI97_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBC2464285.1"
                     /translation="SLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGA
                     GFMYFSVLFFT"
BASE COUNT          660 a          338 c          428 g          503 t
ORIGIN      
        1 gaatgggaaa cgattgaaga tgagattgca gaacttgaag aaaaaatctc attgctccaa
       61 gaagagatga atcatcaagg agataatttc actcgattac aagaattgca aaacgacgta
      121 tctgaaacag aagcacaact tgaagaaaaa atggcacggt gggaatactt gagtgaatgg
      181 gtagaagatt aggaggaaaa aggatggaac aagcgtatct tgacttagga agaaaattgt
      241 tggaagaagg gcatcgtaaa gaagacagaa caggcacagg cacattaagc ttattcggat
      301 accaaatgcg gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa cgagtacctt
      361 ttggattgat caaaagcgaa ttgctttggt ttttaaaagg agatacgaat atccgctatc
      421 tattacaaaa taaaaaccat atttgggatg aatgggcatt tgaacgctac atcaaaagtc
      481 cagactataa tggaccagat atgactgatt ttggccatag atgtttgaca gatcctgaat
      541 tcaatgaaga atataaaaag cagagcaagc ttttttgcga gaagatccta accgatgatt
      601 cctttgctga aacatacggc gatcttggac acatctacgg ctatcaatgg cgtcactggg
      661 aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca atcaaaaaga
      721 ctcctgattc tagaagattg atcgtttctg catggaatcc ggaagatgtt cctagcatgg
      781 ctttgcctcc ttgtcatacg atgtttcaat tttatgttca agagggacgg ctaagctgcc
      841 aattgtatca acgcagtggg gatgtgtttc taggcgttcc gttcaacatt gcaagttatg
      901 cgttgctgac tcacctaatt gcacatgaaa caggtttagc ggttggcgaa tttgttcaca
      961 cattaggaga tgctcatctt tatatcaatc atatcgaaca aatgaaagaa caattgagcc
     1021 gagaaatacg cgcatttccg actttagttt tgaatcagga aaagcagtca gtatttgatt
     1081 ttgagatgga agatatccaa ttagaaggat atgacccaca tccggcgatc aaagctccta
     1141 tcgctgttta gaatcagaaa ggatgagaaa atgttagctg caatttgggc acaggatgaa
     1201 aacggattga ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga tctgaaattc
     1261 ttcaaacaaa tgacagaagc aaatacacta gtgatgggac gaaaaacatt cgaagggatg
     1321 ggtagccgtc cgttgccgaa tcgtcagacg attgttttga cgagagattc ttcctataaa
     1381 gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga aaaagaaaca
     1441 gacggtatct ttttcatcgc tggagggtca gctgtttatc aagaatttct gccattttgt
     1501 acgatattat accgtacagt catccatcat gtattcgatg gagatgctta ttttccacct
     1561 gttgactggt ctgattggtc actgatcaat actagtcaag gagaaatgga tgaaaaaaat
     1621 cgttatccgc atcaatttga aacgtatcag cgtaatgaga atcctgtaaa aagcgggaca
     1681 ggcgaattga cgatataaaa aagctatgga tagactagct gttatttaga gctgtcccat
     1741 ccatagcttt atttttttta ttaagtaaag aataaaacag agaagtacat aaaacctgca
     1801 cctaacataa caaacaaatg ccaaacaaca tgcatatagc ggatagaatt caagctataa
     1861 aagaatgcgc ctatcgtgta agaaagcccg cctgctacca tcaaaataac accagtcatt
     1921 ccaagagag
//
LOCUS       DADXTH010000231         1761 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.160, whole genome shotgun sequence.
ACCESSION   DADXTH010000231 DADXTH010000000
VERSION     DADXTH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.160"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..184
                     /locus_tag="IWI97_002868"
     CDS             <1..184
                     /locus_tag="IWI97_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBC2464286.1"
                     /translation="CKFTLAEHFRKKKLTKTNVKELLEGKETLVKGIKNKEKKPYNAV
                     VKIGEKGYIDFISFSK"
     gene            318..557
                     /locus_tag="IWI97_002869"
     CDS             318..557
                     /locus_tag="IWI97_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBC2464287.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            606..689
                     /locus_tag="IWI97_002870"
     CDS             606..689
                     /locus_tag="IWI97_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBC2464288.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYTDK"
     gene            814..1551
                     /gene="erm(B)"
                     /locus_tag="IWI97_002871"
     CDS             814..1551
                     /gene="erm(B)"
                     /locus_tag="IWI97_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBC2464289.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          688 a          268 c          309 g          496 t
ORIGIN      
        1 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
       61 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
      121 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatat ctttctcaaa
      181 ataaacataa aagcccttta aagagggctt ttatatatta atcacaaatc acttatcaca
      241 aatcacttat cacaaatcac aagtgatttg tgattgttga tgataaaata agaataagaa
      301 gaaatagaaa gaagtgagtg attgtgggaa atttaggcgc acaaaaagaa aaacgaaatg
      361 atacaccaat cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg
      421 acttgcacca tatcataaaa atcgaaacag caaagaatgg cggaaacgta aaagaagtta
      481 tggaaataag acttagaagc aaacttaaga gtgtgttgat agtgcattat cttaaaattt
      541 tgtataatag gaattgaagt taaattagat gctaaaaatt tgtaattaag aaggagggat
      601 tcgtcatgtt ggtattccaa atgcgtaatg tagataaaac atctactgtt ttgaaacaga
      661 ctaaaaacag tgattacaca gataaataaa tacgttagat taattcctac cagtgactaa
      721 tcttatgact ttttaaacag ataactaaaa ttacaaacaa atcgtttaac ttctgtattt
      781 gtttatagat gtaatcactt caggagagat tacatgaaca aaaatataaa atattctcaa
      841 aactttttaa cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa
      901 accgataccg tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa
      961 ataagtaaac aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa
     1021 aaattaaaac tgaatactcg tgtcacttta attcaccaag atattctaca gtttcaattc
     1081 cctaacaaac agaggtataa aattgttggg aatattcctt accatttaag cacacaaatt
     1141 attaaaaaag tggtttttga aagccgtgcg tctgacatct atctgattgt tgaagaagga
     1201 ttctacaagc gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc
     1261 tcgattcagc aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac
     1321 agtgtcttaa taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta
     1381 tatacgtact ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat
     1441 cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag
     1501 caagtattgt ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag
     1561 tcgcttttgt aaatttggaa agttacacgt tactaaaggg aatggagata aattattagg
     1621 tatactactg acagcttcca aggagctaaa gaggtcccta gcgcctacgg ggaatttgta
     1681 tcgataaggg gtacaaattc ccactaagcg ctcgggaccc cttgtaggaa aatgtcctaa
     1741 gtgtggcaac aatattgtat t
//
LOCUS       DADXTH010000232         1062 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.177, whole genome shotgun sequence.
ACCESSION   DADXTH010000232 DADXTH010000000
VERSION     DADXTH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.177"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            63..>1062
                     /gene="aac(6')-Ie"
                     /locus_tag="IWI97_002872"
     CDS             63..>1062
                     /gene="aac(6')-Ie"
                     /locus_tag="IWI97_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HBC2464290.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYI"
BASE COUNT          454 a           81 c          178 g          349 t
ORIGIN      
        1 gtaaaaatga ttataaagat gaaatagtat atgtatatga ttatgaaaaa ggtgataaat
       61 aaatgaatat agttgaaaat gaaatatgta taagaacttt aatagatgat gattttcctt
      121 tgatgttaaa atggttaact gatgaaagag tattagaatt ttatggtggt agagataaaa
      181 aatatacatt agaatcatta aaaaaacatt atacagagcc ttgggaagat gaagttttta
      241 gagtaattat tgaatataac aatgttccta ttggatatgg acaaatatat aaaatgtatg
      301 atgagttata tactgattat cattatccaa aaactgatga gatagtctat ggtatggatc
      361 aatttatagg agagccaaat tattggagta aaggaattgg tacaagatat attaaattga
      421 tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt tattttagac cctcataaaa
      481 ataatccaag agcaataagg gcataccaaa aatctggttt tagaattatt gaagatttgc
      541 cagaacatga attacacgag ggcaaaaaag aagattgtta tttaatggaa tatagatatg
      601 atgataatgc cacaaatgtt aaggcaatga aatatttaat tgagcattac tttgataatt
      661 tcaaagtaga tagtattgaa ataatcggta gtggttatga tagtgtggca tatttagtta
      721 ataatgaata catttttaaa acaaaattta gtactaataa gaaaaaaggt tatgcaaaag
      781 aaaaagcaat atataatttt ttaaatacaa atttagaaac taatgtaaaa attcctaata
      841 ttgaatattc gtatattagt gatgaattat ctatactagg ttataaagaa attaaaggaa
      901 cttttttaac accagaaatt tattctacta tgtcagaaga agaacaaaat ttgttaaaac
      961 gagatattgc cagtttttta agacaaatgc acggtttaga ttatacagat attagtgaat
     1021 gtactattga taataaacaa aatgtattag aagagtatat at
//
LOCUS       DADXTH010000233          857 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51523_2015
            SAMN12639482-rid9883783.guided.186, whole genome shotgun sequence.
ACCESSION   DADXTH010000233 DADXTH010000000
VERSION     DADXTH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639482
            Sequence Read Archive: SRR10030852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742157.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 14:26:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,873
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 10 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639482-rid9883783.guided.186"
                     /isolate="E51523_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            244..792
                     /gene="aac(6')"
                     /locus_tag="IWI97_002873"
     CDS             244..792
                     /gene="aac(6')"
                     /locus_tag="IWI97_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBC2464291.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          306 a          126 c          181 g          244 t
ORIGIN      
        1 gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca
       61 attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat
      121 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
      181 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
      241 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
      301 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
      361 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
      421 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
      481 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
      541 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
      601 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
      661 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
      721 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
      781 aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga
      841 atttacgaaa attactt
//
